Reverse transcriptase

Overview
NameReverse transcriptase
Smed IDSMED30029865
Length (bp)2317
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Reverse transcriptase (SMED30029865) t-SNE clustered cells

Violin plots show distribution of expression levels for Reverse transcriptase (SMED30029865) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Reverse transcriptase (SMED30029865) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Reverse transcriptase (SMED30029865) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30029865

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
pharynxSMED30029865SMESG000079542.1 dd_Smed_v4_78314_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
reproductive organSMED30029865SMESG000079542.1 SMESG000071441.1 SMESG000059599.1 SMESG000047025.1 SMESG000047024.1 SMESG000046893.1 SMESG000041246.1 SMESG000029087.1 SMESG000013749.1 SMESG000010825.1 SMESG000008086.1 SMESG000077611.1 SMESG000073729.1 SMESG000055578.1 SMESG000055415.1 SMESG000052110.1 SMESG000050492.1 SMESG000039167.1 SMESG000036922.1 SMESG000033763.1 SMESG000027381.1 SMESG000070487.1 SMESG000018675.1 SMESG000018320.1 SMESG000011196.1 SMESG000008519.1 Contig44875newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30029865SMESG000079542.1 SMESG000071441.1 SMESG000059599.1 SMESG000047025.1 SMESG000047024.1 SMESG000046893.1 SMESG000041246.1 SMESG000029087.1 SMESG000013749.1 SMESG000010825.1 SMESG000008086.1 SMESG000077611.1 SMESG000073729.1 SMESG000055578.1 SMESG000055415.1 SMESG000052110.1 SMESG000050492.1 SMESG000039167.1 SMESG000036922.1 SMESG000033763.1 SMESG000027381.1 SMESG000070487.1 SMESG000018675.1 SMESG000018320.1 SMESG000011196.1 SMESG000008519.1 Contig44875uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Reverse transcriptase vs. Ensembl Zebrafish
Match: si:dkeyp-88h4.2 (si:dkeyp-88h4.2 [Source:ZFIN;Acc:ZDB-GENE-131121-105])

HSP 1 Score: 68.5514 bits (166), Expect = 8.841e-18
Identity = 50/139 (35.97%), Postives = 70/139 (50.36%), Query Frame = 1 
Query: 1303 LQQNHYENTMPDVWKYFKTLMIPKPDKDDKYNLISSWRPIALLSVVYKIFASILARRLTSWVNRNNLLHI--GQKWGSVHDGCVEHNSVLSSCLENSKYSKDTPTIIAFLDIKDAFDNVPHDYMWKILRYIGVDEKFTN 1713
            LQ+     TM    K     +IPKPDKD    +I +WRPI LL+  YK+ ASI A+RL     + +L +I    + G +    +  N+ L   L + K   D+  II FLD   AFD V H+++   L+  G   KF N
Sbjct:   35 LQECLENETMTPTMKQGVISLIPKPDKDP--TIIDNWRPITLLNFDYKLLASIFAKRL-----KRHLHYIINETQTGFIKGRHISCNTRLVLDLIDYKNEIDSDAIILFLDFYKAFDTVKHEFLLDSLKAFGFPLKFLN 166

HSP 2 Score: 42.3578 bits (98), Expect = 8.841e-18
Identity = 35/135 (25.93%), Postives = 61/135 (45.19%), Query Frame = 2 
Query: 1697 MKNSQTLKLLYTETSSYYTCGPIVTPNIPIKQGVKQGCPLSMILFAIAINTV-LQAVTLSKVKPFKIGESSIQVLAYADDIGLIANNTHDLQNILNIAFDVACEIGFEYRPEKCAYI*LPNVDTIGEISINNIQI 2098
            +K    +++LY + +S        +P  P+ +G++QGCPLS  LF   + T+ +  +  + +    I    I++   ADD  L   +   +  +L I    +   G +    KC  I L   DT+ +  I NI I
Sbjct:  162 LKFLNVVQMLYKDINSCVILNSCTSPRFPVLRGIRQGCPLSPFLFLFVVETLSIDILHNNNLVGINIFNREIRISQLADDTTLFLKDKDQVSPVLTIINAFSDASGLKLNLLKCEIICL--YDTL-DAKIENIPI 293
[Close]
BLAST of Reverse transcriptase vs. Ensembl Zebrafish
Match: CU024870.2 (pep chromosome:GRCz11:25:23489979:23495448:1 gene:ENSDARG00000103841.2 transcript:ENSDART00000158200.2 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:CU024870.2)

HSP 1 Score: 68.5514 bits (166), Expect = 1.215e-17
Identity = 50/139 (35.97%), Postives = 70/139 (50.36%), Query Frame = 1 
Query: 1303 LQQNHYENTMPDVWKYFKTLMIPKPDKDDKYNLISSWRPIALLSVVYKIFASILARRLTSWVNRNNLLHI--GQKWGSVHDGCVEHNSVLSSCLENSKYSKDTPTIIAFLDIKDAFDNVPHDYMWKILRYIGVDEKFTN 1713
            LQ+     TM    K     +IPKPDKD    +I +WRPI LL+  YK+ ASI A+RL     + +L +I    + G +    +  N+ L   L + K   D+  II FLD   AFD V H+++   L+  G   KF N
Sbjct:  500 LQECLENETMTPTMKQGVISLIPKPDKDP--TIIDNWRPITLLNFDYKLLASIFAKRL-----KRHLHYIINETQTGFIKGRHISCNTRLVLDLIDYKNEIDSDAIILFLDFYKAFDTVKHEFLLDSLKAFGFPLKFLN 631

HSP 2 Score: 41.5874 bits (96), Expect = 1.215e-17
Identity = 34/129 (26.36%), Postives = 59/129 (45.74%), Query Frame = 2 
Query: 1715 LKLLYTETSSYYTCGPIVTPNIPIKQGVKQGCPLSMILFAIAINTV-LQAVTLSKVKPFKIGESSIQVLAYADDIGLIANNTHDLQNILNIAFDVACEIGFEYRPEKCAYI*LPNVDTIGEISINNIQI 2098
            +++LY + +S        +P  P+ +G++QGCPLS  LF   + T+ +  +  + +    I    I++   ADD  L   +   +  +L I    +   G +    KC  I L   DT+ +  I NI I
Sbjct:  633 VQMLYKDINSCVILNSCTSPRFPVLRGIRQGCPLSPFLFLFVVETLSIDILHNNNLVGINIFNREIRISQLADDTTLFLKDKDQVSPVLTIINAFSDASGLKLNLLKCEIICL--YDTL-DAKIENIPI 758
[Close]
BLAST of Reverse transcriptase vs. Ensembl Zebrafish
Match: BX544877.2 (pep chromosome:GRCz11:25:25310063:25315481:1 gene:ENSDARG00000103976.2 transcript:ENSDART00000164291.2 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:BX544877.2)

HSP 1 Score: 68.5514 bits (166), Expect = 1.374e-17
Identity = 50/139 (35.97%), Postives = 70/139 (50.36%), Query Frame = 1 
Query: 1303 LQQNHYENTMPDVWKYFKTLMIPKPDKDDKYNLISSWRPIALLSVVYKIFASILARRLTSWVNRNNLLHI--GQKWGSVHDGCVEHNSVLSSCLENSKYSKDTPTIIAFLDIKDAFDNVPHDYMWKILRYIGVDEKFTN 1713
            LQ+     TM    K     +IPKPDKD    +I +WRPI LL+  YK+ ASI A+RL     + +L +I    + G +    +  N+ L   L + K   D+  II FLD   AFD V H+++   L+  G   KF N
Sbjct:  500 LQECLENETMTPTMKQGVISLIPKPDKDP--TIIDNWRPITLLNFDYKLLASIFAKRL-----KRHLHYIINETQTGFIKGRHISCNTRLVLDLIDYKNEIDSDAIILFLDFYKAFDTVKHEFLLDSLKAFGFPLKFLN 631

HSP 2 Score: 41.5874 bits (96), Expect = 1.374e-17
Identity = 34/129 (26.36%), Postives = 59/129 (45.74%), Query Frame = 2 
Query: 1715 LKLLYTETSSYYTCGPIVTPNIPIKQGVKQGCPLSMILFAIAINTV-LQAVTLSKVKPFKIGESSIQVLAYADDIGLIANNTHDLQNILNIAFDVACEIGFEYRPEKCAYI*LPNVDTIGEISINNIQI 2098
            +++LY + +S        +P  P+ +G++QGCPLS  LF   + T+ +  +  + +    I    I++   ADD  L   +   +  +L I    +   G +    KC  I L   DT+ +  I NI I
Sbjct:  633 VQMLYKDINSCVILNSCTSPRFPVLRGIRQGCPLSPFLFLFVVETLSIDILHNNNLVGINIFNREIRISQLADDTTLFLKDKDQVSPVLTIINAFSDASGLKLNLLKCEIICL--YDTL-DAKIENIPI 758
[Close]
BLAST of Reverse transcriptase vs. Ensembl Zebrafish
Match: CU462878.3 (pep chromosome:GRCz11:17:8542203:8545189:1 gene:ENSDARG00000103562.2 transcript:ENSDART00000158873.2 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:CU462878.3)

HSP 1 Score: 62.3882 bits (150), Expect = 7.576e-16
Identity = 40/126 (31.75%), Postives = 72/126 (57.14%), Query Frame = 1 
Query: 1330 MPDVWKYFKTLMIPKPDKDDKYNLISSWRPIALLSVVYKIFASILARRLTSWVNRNNLLHIGQKWGSVHDGCVEHNSVLSSCLENSKYSKDTPTIIAFLDIKDAFDNVPHDYMWKILRYIGVDEKF 1707
            +P  W+     +IPK  KD   N  SS+RPI++L+V YK+FASIL++RL   ++   L+ + Q  G + +   + N   +  + +   +++   ++  LD + AFD+V  DY++++L   G + KF
Sbjct:  319 IPQSWQEAVISIIPKQGKDK--NECSSYRPISVLNVDYKLFASILSKRLEVVISE--LVDLDQT-GFIQNRQTQDNLRRTLQIMSHITTENISAMLLSLDAEKAFDSVGWDYLYQVLARFGFNNKF 439

HSP 2 Score: 41.9726 bits (97), Expect = 7.576e-16
Identity = 22/64 (34.38%), Postives = 37/64 (57.81%), Query Frame = 2 
Query: 1766 VTPNIPIKQGVKQGCPLSMILFAIAINTVLQAVTLS-KVKPFKIGESSIQVLAYADDIGLIANN 1954
            ++  I +++G +QGCPLS  LFA+ I  + QA+    ++K   I +   ++  YADD+ L   N
Sbjct:  460 LSKTIYLERGTRQGCPLSPTLFALFIEPLAQAIREEPEIKGIVIKKEEHKICMYADDVLLFLAN 523
[Close]
BLAST of Reverse transcriptase vs. Ensembl Zebrafish
Match: BX927081.1 (pep chromosome:GRCz11:6:24296039:24301324:-1 gene:ENSDARG00000105300.2 transcript:ENSDART00000171401.2 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:BX927081.1)

HSP 1 Score: 58.5362 bits (140), Expect = 2.158e-15
Identity = 38/134 (28.36%), Postives = 66/134 (49.25%), Query Frame = 1 
Query: 1330 MPDVWKYFKTLMIPKPDKDDKYNLISSWRPIALLSVVYKIFASILARRLTSWVNRNNLLHIGQKWGSVHDGCVEHNSVLSSCLENSKYSKDTPTIIAFLDIKDAFDNVPHDYMWKILRYIGVDEKFTNFETSIH 1731
            +P   K     ++PKP+KD    ++ +WRPI LL+V YK+ + I A+RL   +N    +    + G +    +  N  L   L +     ++  ++ FLD   AFD + H +M+  L+Y G  ++F +     H
Sbjct:  509 LPTSLKQGLITLLPKPNKD--LMILDNWRPITLLNVDYKLLSLIYAKRLKEGLNE---IISEYQTGFMAGRHISWNIRLILDLLDYSNLIESEALLLFLDFHKAFDTIEHQFMFMALKYFGFGDRFISIMELFH 637

HSP 2 Score: 44.2838 bits (103), Expect = 2.158e-15
Identity = 29/115 (25.22%), Postives = 51/115 (44.35%), Query Frame = 2 
Query: 1715 LKLLYTETSSYYTCGPIVTPNIPIKQGVKQGCPLSMILFAIAINTV-LQAVTLSKVKPFKIGESSIQVLAYADDIGLIANNTHDLQNILNIAFDVACEIGFEYRPEKCAYI*LPN 2056
            ++L + + +S     P  +   PI +GV+QGCP++  LF + +  + +  +  S ++   I    I++   ADD  L   +   L   L +  D +   G      KC  I L N
Sbjct:  633 MELFHRDINSSINLYPNTSKRFPICRGVRQGCPIAPFLFLLVVEFLSIYVLKSSAIQGITIFNKEIRISQLADDTVLFLKDKDQLPAALQLVNDFSIASGLTLNVTKCEIISLYN 747
[Close]
BLAST of Reverse transcriptase vs. Ensembl Xenopus
Match: ENSXETT00000040456.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:8:87387561:87390644:-1 gene:ENSXETG00000021299.1 transcript:ENSXETT00000040456.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 59.3066 bits (142), Expect = 2.162e-16
Identity = 40/123 (32.52%), Postives = 65/123 (52.85%), Query Frame = 1 
Query: 1360 LMIPKPDKDDKYNLISSWRPIALLSVVYKIFASILARRL----TSWVNRNNLLHIGQKWGSVHDGCVEHNSVLSSCLENSKYSKDTPTIIAFLDIKDAFDNVPHDYMWKILRYIGVDEKFTNF 1716
            +++PK  KD   +L  S+RPI+LL+   KIFA +LARRL    T  ++ + +  I  K  +++   +  N   +S  + +K        IA LDI  AFD V   Y+W++L + G  +K+   
Sbjct:  141 VLLPKQGKDP--HLCESFRPISLLTADVKIFAKVLARRLSRVITKIIHPDQIGFIPAKTAALNTRRLYLNLAHASAGQGAK-------AIAALDITKAFDTVEWPYLWQVLTHFGFGKKYIQM 254

HSP 2 Score: 47.7506 bits (112), Expect = 2.162e-16
Identity = 28/76 (36.84%), Postives = 41/76 (53.95%), Query Frame = 2 
Query: 1709 QTLKLLYTETSSYYTCGPIVTPNIPIKQGVKQGCPLSMILFAIAINTVLQAVTLS-KVKPFKIGESSIQVLAYADD 1933
            Q ++LLY    +      + TP   + +G +QGCPLS +LFAIAI    QAV    ++  F+    + +V  YADD
Sbjct:  253 QMVQLLYKYPVASIRINSLTTPAFALSRGTRQGCPLSPLLFAIAIEPFAQAVRAHPQITGFEYPGFNEKVQLYADD 328
[Close]
BLAST of Reverse transcriptase vs. Ensembl Xenopus
Match: slc7a1 (solute carrier family 7 (cationic amino acid transporter, y+ system), member 1 [Source:Xenbase;Acc:XB-GENE-984439])

HSP 1 Score: 54.299 bits (129), Expect = 2.209e-16
Identity = 30/100 (30.00%), Postives = 56/100 (56.00%), Query Frame = 2 
Query: 1709 QTLKLLYTETSSYYTCGPIVTPNIPIKQGVKQGCPLSMILFAIAINTVLQAVTLSKVKPFKIGES-SIQVLAYADDIGLIANNTHDLQNILNIAFDVACE 2005
            + L +LY E  S+         N  ++ GV+QGCPLS +L+  AI+  L+AV    ++ F+      ++++AYADD+ ++ ++  ++ N+L  A +   E
Sbjct:  337 EWLTVLYREGESFPLVNGWRGENFGVEAGVRQGCPLSPLLYVFAIDPFLRAVQALDLEGFRAPRCLPLKLVAYADDVTVVVSHPEEV-NLLTDAIESYSE 435

HSP 2 Score: 52.373 bits (124), Expect = 2.209e-16
Identity = 33/114 (28.95%), Postives = 54/114 (47.37%), Query Frame = 1 
Query: 1402 ISSWRPIALLSVVYKIFASILARRL----TSWVNRNNLLHIGQKWGSVHDGCVEHNSVLSSCLENSKYSKDTPTIIAFLDIKDAFDNVPHDYMWKILRYIGVDEKFTNFETSIH 1731
            + +WRPIALL+   K+ A +L  RL     + ++R     +  K  S+    +    VL +C   S     +   +  LD   AFD V H Y+W  LR  G+ ++F  + T ++
Sbjct:  237 VKNWRPIALLNTDRKLLAKVLFSRLILFSGTLLSREQFCTV--KGRSIFGALLSIREVLETCKAQS-----SRRYVVGLDQAKAFDRVDHQYLWATLRKYGIPDQFVEWLTVLY 343
[Close]
BLAST of Reverse transcriptase vs. Ensembl Xenopus
Match: ENSXETT00000032317.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:1:130520927:130524992:-1 gene:ENSXETG00000008594.1 transcript:ENSXETT00000032317.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 58.9214 bits (141), Expect = 2.612e-16
Identity = 40/123 (32.52%), Postives = 65/123 (52.85%), Query Frame = 1 
Query: 1360 LMIPKPDKDDKYNLISSWRPIALLSVVYKIFASILARRL----TSWVNRNNLLHIGQKWGSVHDGCVEHNSVLSSCLENSKYSKDTPTIIAFLDIKDAFDNVPHDYMWKILRYIGVDEKFTNF 1716
            +++PK  KD   +L  S+RPI+LL+   KIFA +LARRL    T  ++ + +  I  K  +++   +  N   +S  + +K        IA LDI  AFD V   Y+W++L + G  +K+   
Sbjct:  511 VLLPKQGKDP--HLCESFRPISLLTADVKIFAKVLARRLSRVITKIIHPDQIGFIPAKTAALNTRRLYLNLAHASAGQGAK-------AIAALDITKAFDTVEWPYLWQVLTHFGFGKKYIQM 624

HSP 2 Score: 47.7506 bits (112), Expect = 2.612e-16
Identity = 29/79 (36.71%), Postives = 42/79 (53.16%), Query Frame = 2 
Query: 1700 KNSQTLKLLYTETSSYYTCGPIVTPNIPIKQGVKQGCPLSMILFAIAINTVLQAVTL-SKVKPFKIGESSIQVLAYADD 1933
            K  Q ++LLY    +      + TP   + +G +QGCPLS +LFAIAI    QAV    ++  F+    + +V  YADD
Sbjct:  620 KYIQMVQLLYKYPVASIRINSLTTPAFALSRGTRQGCPLSPLLFAIAIEPFAQAVRAHPQITGFEYPGFNEKVQLYADD 698
[Close]
BLAST of Reverse transcriptase vs. Ensembl Xenopus
Match: ENSXETT00000002428.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:3:15907829:15913358:1 gene:ENSXETG00000006437.1 transcript:ENSXETT00000002428.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 61.2326 bits (147), Expect = 3.624e-16
Identity = 43/133 (32.33%), Postives = 67/133 (50.38%), Query Frame = 1 
Query: 1330 MPDVWKYFKTLMIPKPDKDDKYNLISSWRPIALLSVVYKIFASILARRLT----SWVNRNNLLHIGQKWGSVHDGCVEHNSVLSSCLENSKYSKDTPTIIAFLDIKDAFDNVPHDYMWKILRYIGVDEKFTNF 1716
            +P+       +++PKP KD +  L  S+RPI+LL+   KI+A ILARRL     + VN + +  I  K  +++   +  N  L+     ++        IA LDI  AFD V   Y+W +L+ +G    F N 
Sbjct:  501 LPESMYEAAIILLPKPGKDPQ--LCESFRPISLLTTDVKIYAKILARRLARVIKTLVNPDQIGFIPTKTTALNTRRLYLNLSLTPSNTGNR-------AIAALDIAKAFDTVEWSYLWCVLKQLGFGPTFINM 624

HSP 2 Score: 45.0542 bits (105), Expect = 3.624e-16
Identity = 24/74 (32.43%), Postives = 41/74 (55.41%), Query Frame = 2 
Query: 1715 LKLLYTETSSYYTCGPIVTPNIPIKQGVKQGCPLSMILFAIAINTVLQAVTLSK-VKPFKIGESSIQVLAYADD 1933
            ++LLY    +      + T N  + +G +QGCPLS +LFA+AI    QAV     ++ +++  +  ++  YADD
Sbjct:  625 VQLLYKSPRATLRINSLCTSNFTLSRGTRQGCPLSPLLFALAIEPFAQAVRKHPLIQGYELKGTIEKIQLYADD 698
[Close]
BLAST of Reverse transcriptase vs. Ensembl Xenopus
Match: ENSXETT00000046003.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:KV460539.1:49812:51785:-1 gene:ENSXETG00000019210.1 transcript:ENSXETT00000046003.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 58.5362 bits (140), Expect = 1.065e-15
Identity = 42/139 (30.22%), Postives = 72/139 (51.80%), Query Frame = 1 
Query: 1297 SSLQQNHYENTMPDVWKYFKTLMIPKPDKDDKYNLISSWRPIALLSVVYKIFASILARRLTSWVNRNNLLHIGQKWGSVHDGCVEHNSVLSSCLENSK--YSKDTPTIIAFLDIKDAFDNVPHDYMWKILRYIGVDEKF 1707
            ++LQ      ++P  +     ++IPK  +D    L SS+RPI+L++   KI A +LA RLT  V   +L+H  Q  G +     + N  L     N +  ++      +A LD + AFD++  +Y+W++LR  G+  +F
Sbjct:  326 TTLQAAWDSQSLPPSFAEALIVVIPKAGRDP--TLCSSYRPISLINTDAKILAKVLATRLTRAVQ--DLIHPDQS-GFMPGRATDFN--LRRLFTNLQITHTNSGARAVASLDSEKAFDSIEWEYLWEVLRRFGLGARF 457

HSP 2 Score: 46.2098 bits (108), Expect = 1.065e-15
Identity = 27/79 (34.18%), Postives = 41/79 (51.90%), Query Frame = 2 
Query: 1709 QTLKLLYTETSSYYTCGPIVTPNIPIKQGVKQGCPLSMILFAIAINTVLQAVTLS---KVKPFKIGESSIQVLAYADDI 1936
            Q LK+LY    +       V+P   + +G +QGCPLS  LFA+AI  +  A+T+     +K       + +V  +ADDI
Sbjct:  459 QWLKMLYKHPIARVRVNNTVSPTFSLHRGTRQGCPLSPTLFALAIEPL--AITIRNNPNIKGLNFANVTEKVSLFADDI 535
[Close]
BLAST of Reverse transcriptase vs. UniProt/SwissProt
Match: sp|Q03278|PO21_NASVI (Retrovirus-related Pol polyprotein from type-1 retrotransposable element R2 (Fragment) OS=Nasonia vitripennis OX=7425 PE=4 SV=2)

HSP 1 Score: 55.0694 bits (131), Expect = 1.315e-14
Identity = 41/134 (30.60%), Postives = 67/134 (50.00%), Query Frame = 1 
Query: 1318 YENTMPDVWKYFKTLMIPK-PDKDDKYNLISSWRPIALLSVVYKIFASILARRLTSWVNRNNLLHIGQKWGSVHDGCVEHNSVLSSCLENSKYSKDTPTIIAFLDIKDAFDNVPHDYMWKILRYIGVDEKFTNF 1716
            Y    P  +   +T++IPK P   D     + +RP+++ SV  + F  ILA R    +  + LL   Q+   V DG  E+ S+LS+ ++ ++  K     IA LD+K AFD+V H  +   LR   +  +  N+
Sbjct:  361 YHGQCPRRYLDSRTVLIPKEPGTMDP----ACFRPLSIASVALRHFHRILANR----IGEHGLLDTRQRAFIVADGVAENTSLLSAMIKEARM-KIKGLYIAILDVKKAFDSVEHRSILDALRRKKLPLEMRNY 485

HSP 2 Score: 47.7506 bits (112), Expect = 1.315e-14
Identity = 30/82 (36.59%), Postives = 42/82 (51.22%), Query Frame = 2 
Query: 1790 QGVKQGCPLSMILFAIAINTVLQAVTLSKVKPFKIGESSIQVLAYADDIGLIANNTHDLQNILNIAFDVACEIGFEYRPEKC 2035
            +GV+QG PLS +LF   ++ VL+   L +   F +G   I  L +ADD+ L+A     LQ  L+       E G E  P KC
Sbjct:  511 RGVRQGDPLSPLLFNCVMDAVLR--RLPENTGFLMGAEKIGALVFADDLVLLAETREGLQASLSRIEAGLQEQGLEMMPRKC 590
[Close]
BLAST of Reverse transcriptase vs. UniProt/SwissProt
Match: sp|Q03274|PO22_POPJA (Retrovirus-related Pol polyprotein from type-1 retrotransposable element R2 (Fragment) OS=Popillia japonica OX=7064 PE=4 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 6.424e-14
Identity = 41/141 (29.08%), Postives = 75/141 (53.19%), Query Frame = 1 
Query: 1303 LQQNHYENTMPDVWKYFKTLMIPKPDKDDKYNLISSWRPIALLSVVYKIFASILARRLTSWVNRNNLLHIGQKWGSVHDGCVEHNSVLSSCLENSKYSKDTPTIIAFLDIKDAFDNVPHDYMWKILRYIGVDEKFTNFETS 1725
            +Q +     +P  W   +T +IPK   D      S+WRPI + S + ++   ILA+RL + V     LH  QK  +  DG + ++ +L + + + +  + T  +++ LD++ AFD V H  + + L+ +G+DE  +N+ T 
Sbjct:   43 VQLHLLRGHVPTPWTAMRTTLIPK---DGDLENPSNWRPITIASALQRLLHRILAKRLEAAVE----LHPAQKGYARIDGTLVNSLLLDTYISSRREQRKTYNVVS-LDVRKAFDTVSHSSICRALQRLGIDEGTSNYITG 175

HSP 2 Score: 30.4166 bits (67), Expect = 6.424e-14
Identity = 19/49 (38.78%), Postives = 27/49 (55.10%), Query Frame = 2 
Query: 1769 TPNIPIKQGVKQGCPLSMILFAIAINTVLQAVTLSKVKPFKIGESSIQV 1915
            T  I I++GVKQG PLS  LF   ++ +L ++  +      IGE  I V
Sbjct:  192 TRKICIRRGVKQGDPLSPFLFNAVLDELLCSLQSTPGIGGTIGEEKIPV 240
[Close]
BLAST of Reverse transcriptase vs. UniProt/SwissProt
Match: sp|P11369|LORF2_MOUSE (LINE-1 retrotransposable element ORF2 protein OS=Mus musculus OX=10090 GN=Pol PE=1 SV=2)

HSP 1 Score: 50.447 bits (119), Expect = 8.499e-14
Identity = 27/73 (36.99%), Postives = 43/73 (58.90%), Query Frame = 2 
Query: 1778 IPIKQGVKQGCPLSMILFAIAINTVLQAVTLSK-VKPFKIGESSIQVLAYADD----IGLIANNTHDLQNILN 1981
            IP+K G +QGCPLS  LF I +  + +A+   K +K  +IG+  +++   ADD    I    N+T +L N++N
Sbjct:  658 IPLKSGTRQGCPLSPYLFNIVLEVLARAIRQQKEIKGIQIGKEEVKISLLADDMIVYISDPKNSTRELLNLIN 730

HSP 2 Score: 49.6766 bits (117), Expect = 8.499e-14
Identity = 37/143 (25.87%), Postives = 65/143 (45.45%), Query Frame = 1 
Query: 1321 ENTMPDVWKYFKTLMIPKPDKDDKYNLISSWRPIALLSVVYKIFASILARRLTSWV------NRNNLLHIGQKWGSVHDGCVEHNSVLSSCLENSKYSKDTPTIIAFLDIKDAFDNVPHDYMWKILRYIGVDEKFTNFETSIH 1731
            E T+P+ +      +IPKP KD     I ++RPI+L+++  KI   ILA R+   +      ++   +   Q W ++            + +      KD   +I  LD + AFD + H +M K+L   G+   + N   +I+
Sbjct:  509 EGTLPNSFYEATITLIPKPQKDP--TKIENFRPISLMNIDAKILNKILANRIQEHIKAIIHPDQVGFIPGMQGWFNIRKSI--------NVIHYINKLKDKNHMIISLDAEKAFDKIQHPFMIKVLERSGIQGPYLNMIKAIY 641
[Close]
BLAST of Reverse transcriptase vs. UniProt/SwissProt
Match: sp|P08548|LIN1_NYCCO (LINE-1 reverse transcriptase homolog OS=Nycticebus coucang OX=9470 PE=4 SV=1)

HSP 1 Score: 49.6766 bits (117), Expect = 5.559e-13
Identity = 38/153 (24.84%), Postives = 71/153 (46.41%), Query Frame = 1 
Query: 1303 LQQNHYENTMPDVWKYFKTLMIPKPDKDDKYNLISSWRPIALLSVVYKIFASILARRLTSWVNRNNLLH---IG-----QKWGSVHDG--CVEHNSVLSSCLENSKYSKDTPTIIAFLDIKDAFDNVPHDYMWKILRYIGVDEKFTNFETSIH 1731
             Q    E  +P+ +      +IPKP KD       ++RPI+L+++  KI   IL  R+   + +  ++H   +G     Q W ++      ++H + L          K+   +I  +D + AFDN+ H +M + L+ IG++  F     +I+
Sbjct:  495 FQNIEKEGILPNTFYEANITLIPKPGKDPTRK--ENYRPISLMNIDAKILNKILTNRIQQHIKK--IIHHDQVGFIPGSQGWFNIRKSINVIQHINKL----------KNKDHMILSIDAEKAFDNIQHPFMIRTLKKIGIEGTFLKLIEAIY 633

HSP 2 Score: 47.7506 bits (112), Expect = 5.559e-13
Identity = 24/86 (27.91%), Postives = 47/86 (54.65%), Query Frame = 2 
Query: 1709 QTLKLLYTETSSYYTCGPIVTPNIPIKQGVKQGCPLSMILFAIAINTVLQAVTLSK-VKPFKIGESSIQVLAYADDIGLIANNTHD 1963
            + ++ +Y++ ++      +   + P++ G +QGCPLS +LF I +  +  A+   K +K   IG   I++  +ADD+ +   NT D
Sbjct:  627 KLIEAIYSKPTANIILNGVKLKSFPLRSGTRQGCPLSPLLFNIVMEVLAIAIREEKAIKGIHIGSEEIKLSLFADDMIVYLENTRD 712
[Close]
BLAST of Reverse transcriptase vs. UniProt/SwissProt
Match: sp|P16423|POLR_DROME (Retrovirus-related Pol polyprotein from type-2 retrotransposable element R2DM OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 48.9062 bits (115), Expect = 9.724e-13
Identity = 29/82 (35.37%), Postives = 44/82 (53.66%), Query Frame = 2 
Query: 1790 QGVKQGCPLSMILFAIAINTVLQAVTLSKVKPFKIGESSIQVLAYADDIGLIANNTHDLQNILNIAFDVACEIGFEYRPEKC 2035
            +GVKQG PLS ILF + ++ +L+  TL      K+G +     A+ADD+ L A     LQ +L+   D    +G +   +KC
Sbjct:  537 RGVKQGDPLSPILFNLVMDRLLR--TLPSEIGAKVGNAITNAAAFADDLVLFAETRMGLQVLLDKTLDFLSIVGLKLNADKC 616

HSP 2 Score: 47.7506 bits (112), Expect = 9.724e-13
Identity = 35/131 (26.72%), Postives = 64/131 (48.85%), Query Frame = 1 
Query: 1324 NTMPDVWKYFKTLMIPKPDKDDKYNLISSWRPIALLSVVYKIFASILARRLTSWVNRNNLLHIGQKWGSVHDGCVEHNSVLSSCLENSKYSKDTPTIIAFLDIKDAFDNVPHDYMWKILRYIGVDEKFTNF 1716
              +P   +  +T+ IPK     +      +RPI++ SV+ +   +ILA RL S +N +      Q+     DGC ++ +++   L +S +       IA LD+  AFD++ H  ++  LR  G  + F ++
Sbjct:  389 GNLPHSIRLARTVFIPKTVTAKRPQ---DFRPISVPSVLVRQLNAILATRLNSSINWDPR----QRGFLPTDGCADNATIVDLVLRHS-HKHFRSCYIANLDVSKAFDSLSHASIYDTLRAYGAPKGFVDY 511
[Close]
BLAST of Reverse transcriptase vs. TrEMBL
Match: A0A159X4Q6 (ORF2-like protein (Fragment) OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 125.946 bits (315), Expect = 6.542e-35
Identity = 60/97 (61.86%), Postives = 72/97 (74.23%), Query Frame = 1 
Query: 1330 MPDVWKYFKTLMIPKPDKDDKYNLISSWRPIALLSVVYKIFASILARRLTSWVNRNNLLHIGQKWGSVHDGCVEHNSVLSSCLENSKYSKDTPTIIA 1620
            +P+ W  FKT++IPKP K   Y+  SSWRPIALLS  YKIF SIL+RRLT W+  N LLH GQK GS  +GCVEHN+VL++ LE+SKYSK  P  IA
Sbjct:  223 IPNDWLSFKTILIPKPGKSGDYDDTSSWRPIALLSTSYKIFTSILSRRLTKWIIENKLLHPGQKGGSRFEGCVEHNAVLTAVLEHSKYSKKAPLAIA 319

HSP 2 Score: 53.1434 bits (126), Expect = 6.542e-35
Identity = 29/56 (51.79%), Postives = 38/56 (67.86%), Query Frame = 2 
Query: 1154 KISKLFYLDQL*I-EVIFELKRADNTSPGIDGIKYYDIAIFDPEGKLLTLLYNKII 1318
            K  ++  LDQ+   EVIFE K+A+ +SPG D I Y D+ + DPEGKLL  L+N II
Sbjct:  163 KSKEIIELDQITAKEVIFEYKQANASSPGDDNITYRDLRLLDPEGKLLARLFNIII 218
[Close]
BLAST of Reverse transcriptase vs. TrEMBL
Match: A0A3L8D8E5 (Reverse transcriptase domain-containing protein OS=Ooceraea biroi OX=2015173 GN=DMN91_010823 PE=4 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 5.116e-33
Identity = 47/131 (35.88%), Postives = 72/131 (54.96%), Query Frame = 1 
Query: 1324 NTMPDVWKYFKTLMIPKPDKDDKYNLISSWRPIALLSVVYKIFASILARRLTSWVNRNNLLHIGQKWGSVHDGCVEHNSVLSSCLENSKYSKDTPTIIAFLDIKDAFDNVPHDYMWKILRYIGVDEKFTNF 1716
              +P  WK   T+++ K  K D+ +L  SWRP+AL   + K+FA++ A RLT WV  N+ L   QK     +GC EHN VL   L +++ ++    ++A+LD+ +AF +VPH  +   L   GV     N 
Sbjct:  694 EAVPPSWKTSNTVLVYK--KGDRDSL-ESWRPLALGDTMPKLFAAVAADRLTDWVIANDKLCRAQKGFLRDEGCYEHNFVLQEILTDARRTRRH-AVVAWLDLSNAFGSVPHAAIRSALVRAGVPSGLINI 820

HSP 2 Score: 68.9366 bits (167), Expect = 5.116e-33
Identity = 36/93 (38.71%), Postives = 55/93 (59.14%), Query Frame = 2 
Query: 1769 TPNIPIKQGVKQGCPLSMILFAIAINTVLQAVT-LSKVKPFKIGESSIQVLAYADDIGLIANNTHDLQNILNIAFDVACEIGFEYRPEKCAYI 2044
            T  IPI+ GV+QGCPLS I+F + I++V++A   L+ V  + I   +  +LAYADD+ LIA     ++ +L      A  +G  + P KCA +
Sbjct:  839 TAPIPIRSGVRQGCPLSPIIFDLVIDSVVRAAAELADVG-YDILGQTFNILAYADDLALIARTPEGMRQLLTAVELEAGRVGLHFNPAKCASL 930

HSP 3 Score: 44.669 bits (104), Expect = 5.116e-33
Identity = 19/39 (48.72%), Postives = 25/39 (64.10%), Query Frame = 2 
Query: 1193 EVIFELKRADNTSPGIDGIKYYDIAIFDPEGKLLTLLYN 1309
            EV   L+R  N++PG DGI Y D+   DP  +LLT L+N
Sbjct:  650 EVDRRLRRMTNSAPGPDGIAYNDLRAADPGARLLTALFN 688
[Close]
BLAST of Reverse transcriptase vs. TrEMBL
Match: A0A3L8D9A5 (Reverse transcriptase domain-containing protein OS=Ooceraea biroi OX=2015173 GN=DMN91_011136 PE=4 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 5.287e-33
Identity = 47/131 (35.88%), Postives = 72/131 (54.96%), Query Frame = 1 
Query: 1324 NTMPDVWKYFKTLMIPKPDKDDKYNLISSWRPIALLSVVYKIFASILARRLTSWVNRNNLLHIGQKWGSVHDGCVEHNSVLSSCLENSKYSKDTPTIIAFLDIKDAFDNVPHDYMWKILRYIGVDEKFTNF 1716
              +P  WK   T+++ K  K D+ +L  SWRP+AL   + K+FA++ A RLT WV  N+ L   QK     +GC EHN VL   L +++ ++    ++A+LD+ +AF +VPH  +   L   GV     N 
Sbjct:  807 EAVPPSWKTSNTVLVYK--KGDRDSL-ESWRPLALGDTMPKLFAAVAADRLTDWVIANDKLCRAQKGFLRDEGCYEHNFVLQEILTDARRTRRH-AVVAWLDLSNAFGSVPHAAIRSALVRAGVPSGLINI 933

HSP 2 Score: 68.5514 bits (166), Expect = 5.287e-33
Identity = 36/93 (38.71%), Postives = 55/93 (59.14%), Query Frame = 2 
Query: 1769 TPNIPIKQGVKQGCPLSMILFAIAINTVLQAVT-LSKVKPFKIGESSIQVLAYADDIGLIANNTHDLQNILNIAFDVACEIGFEYRPEKCAYI 2044
            T  IPI+ GV+QGCPLS I+F + I++V++A   L+ V  + I   +  +LAYADD+ LIA     ++ +L      A  +G  + P KCA +
Sbjct:  952 TAPIPIRSGVRQGCPLSPIIFDLVIDSVVRAAAELADVG-YDILGQTFNILAYADDLALIARTPEGMRQLLTAVELEAGRVGLHFNPAKCASL 1043

HSP 3 Score: 44.669 bits (104), Expect = 5.287e-33
Identity = 19/39 (48.72%), Postives = 25/39 (64.10%), Query Frame = 2 
Query: 1193 EVIFELKRADNTSPGIDGIKYYDIAIFDPEGKLLTLLYN 1309
            EV   L+R  N++PG DGI Y D+   DP  +LLT L+N
Sbjct:  763 EVDRRLRRMTNSAPGPDGIAYSDLRAADPGARLLTALFN 801
[Close]
BLAST of Reverse transcriptase vs. TrEMBL
Match: A0A3L8DAI7 (Reverse transcriptase domain-containing protein OS=Ooceraea biroi OX=2015173 GN=DMN91_010948 PE=4 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 5.861e-33
Identity = 47/135 (34.81%), Postives = 74/135 (54.81%), Query Frame = 1 
Query: 1330 MPDVWKYFKTLMI-PKPDKDDKYNLISSWRPIALLSVVYKIFASILARRLTSWVNRNNLLHIGQKWGSVHDGCVEHNSVLSSCLENSKYSKDTPTIIAFLDIKDAFDNVPHDYMWKILRYIGVDEKFTNFETSIH 1731
            +P  WK   T+++  K D+D     + SWRP+AL   + K+FA++ A RLT WV  N+ L   QK     +GC EHN VL   L +++ ++    ++A+LD+ +AF +VPH  +   L   GV     N   S++
Sbjct:  921 VPPSWKTSNTVLVYKKGDRDS----LESWRPLALGDTMPKLFAAVAADRLTDWVIANDKLCRAQKGFLRDEGCYEHNFVLQEILTDARRTR-RHAVVAWLDLSNAFGSVPHAAIRSALVRAGVPSGLINIWGSMY 1050

HSP 2 Score: 68.5514 bits (166), Expect = 5.861e-33
Identity = 36/93 (38.71%), Postives = 55/93 (59.14%), Query Frame = 2 
Query: 1769 TPNIPIKQGVKQGCPLSMILFAIAINTVLQAVT-LSKVKPFKIGESSIQVLAYADDIGLIANNTHDLQNILNIAFDVACEIGFEYRPEKCAYI 2044
            T  IPI+ GV+QGCPLS I+F + I++V++A   L+ V  + I   +  +LAYADD+ LIA     ++ +L      A  +G  + P KCA +
Sbjct: 1064 TAPIPIRSGVRQGCPLSPIIFDLVIDSVVRAAAELTDVG-YDILGQTFNILAYADDLALIARTPEGMRQLLTAVELEAGRVGLHFNPAKCASL 1155

HSP 3 Score: 44.669 bits (104), Expect = 5.861e-33
Identity = 19/39 (48.72%), Postives = 25/39 (64.10%), Query Frame = 2 
Query: 1193 EVIFELKRADNTSPGIDGIKYYDIAIFDPEGKLLTLLYN 1309
            EV   L+R  N++PG DGI Y D+   DP  +LLT L+N
Sbjct:  875 EVDRRLRRMTNSAPGPDGIAYNDLRAADPGARLLTALFN 913
[Close]
BLAST of Reverse transcriptase vs. TrEMBL
Match: U4USZ6 (Reverse transcriptase domain-containing protein (Fragment) OS=Dendroctonus ponderosae OX=77166 GN=D910_01513 PE=4 SV=1)

HSP 1 Score: 80.8777 bits (198), Expect = 8.150e-33
Identity = 47/112 (41.96%), Postives = 66/112 (58.93%), Query Frame = 2 
Query: 1715 LKLLYTE--TSSYYTCGPIVTPNIPIKQGVKQGCPLSMILFAIAINTVLQAVTLSKVKPFKIGESSIQVLAYADDIGLIANNTHDLQNILNIAFDVACEIGFEYRPEKCAYI 2044
            ++LLY    TS   + GP  TP I IK GVKQGCPLS I+F +AI  +L A+T      +++    I  LAYADD+ +IA N  DLQ +L+   ++A   G  +  +KCA +
Sbjct:  582 IRLLYENNTTSIRSSTGP--TPAIHIKSGVKQGCPLSPIIFNLAIEPILHAITACGGG-YRLMGKKINSLAYADDMAIIAENPSDLQRLLDTTTEMANWAGLSFNTKKCATL 690

HSP 2 Score: 80.4925 bits (197), Expect = 8.150e-33
Identity = 41/123 (33.33%), Postives = 71/123 (57.72%), Query Frame = 1 
Query: 1333 PDVWKYFKTLMIPKPDKDDKYNLISSWRPIALLSVVYKIFASILARRLTSWVNRNNLLHIGQKWGSVHDGCVEHNSVLSSCLENSKYSKDTPTIIAFLDIKDAFDNVPHDYMWKILRYIGVDE 1701
            P  W    T++I    K      +++WRPI+L + + KI  S+LA RL +W  RN+ L   QK     DGC EHN VL S + +++ ++     +A+LD+ +AF ++PH+ +++ L + G+ +
Sbjct:  458 PKAWTTSLTILI---HKKGSREDVTNWRPISLSNTIAKIHTSVLAERLGAWATRNSRLGESQKGFLQMDGCAEHNFVLQSIIADARRNRRQ-CCVAWLDLANAFGSIPHETIFQSLGWSGLSD 576

HSP 3 Score: 31.5722 bits (70), Expect = 8.150e-33
Identity = 13/39 (33.33%), Postives = 22/39 (56.41%), Query Frame = 2 
Query: 1193 EVIFELKRADNTSPGIDGIKYYDIAIFDPEGKLLTLLYN 1309
            EV   + R  NT+PG DG++Y +   FD   +++  + N
Sbjct:  411 EVWKRIIRCSNTAPGPDGVRYSNWRKFDVGAQIIAAVLN 449
[Close]
BLAST of Reverse transcriptase vs. Ensembl Cavefish
Match: ENSAMXT00000034560.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000712.1:195631:202616:-1 gene:ENSAMXG00000042811.1 transcript:ENSAMXT00000034560.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 60.8474 bits (146), Expect = 2.919e-21
Identity = 32/126 (25.40%), Postives = 63/126 (50.00%), Query Frame = 1 
Query: 1324 NTMPDVWKYFKTLMIPKPDKDDKYNLISSWRPIALLSVVYKIFASILARRLTSWVNRNNLLHIGQKWGSVHDGCVEHNSVLSSCLENSKYSKDTPTIIAFLDIKDAFDNVPHDYMWKILRYIGVDE 1701
              +P  +K  +T +IPK    +    + +WRPI + S++ ++F+ I+A RL     R   ++  Q+      GC E+  VL   + + K  +    ++ F+D   AFD++ H+++   L    +D+
Sbjct:  475 GVVPKAFKECRTTLIPKSTSKEALEEVGNWRPITIGSLILRLFSKIMAERLA----RACPINPRQRGFISAPGCAENLKVLQGLIGHCKKERSQLAVV-FVDFARAFDSISHEHILSALGQRQLDQ 595

HSP 2 Score: 52.373 bits (124), Expect = 2.919e-21
Identity = 34/91 (37.36%), Postives = 58/91 (63.74%), Query Frame = 2 
Query: 1769 TPNIPIKQGVKQGCPLSMILFAIAINTVLQAVTLSKVKPFKIGESSIQVLAYADDIGLIANNTHDLQNILNIA-FDVACE-IGFEYRPEKC 2035
            +P+I +K GVKQG  +S ILF +A++ ++Q++  +    +KIG SS+  LA+ADD+ L++++   +++  NIA  D  CE  G   +  KC
Sbjct:  619 SPDILMKVGVKQGDSMSPILFNLAMDPLIQSLE-NLGSGYKIGGSSVTTLAFADDLVLLSSSWDGMRS--NIALLDEFCERTGLRVQSRKC 706

HSP 3 Score: 28.8758 bits (63), Expect = 2.919e-21
Identity = 15/38 (39.47%), Postives = 23/38 (60.53%), Query Frame = 2 
Query: 1193 EVIFELKRADNTSPGIDGIKYYDIAIFDPEGKLLTLLY 1306
            E + ++KR  NT+PG D I    ++ +DP G+ L  LY
Sbjct:  433 ENLGQIKR--NTAPGPDRITRQMLSDWDPSGEKLERLY 468
[Close]
BLAST of Reverse transcriptase vs. Ensembl Cavefish
Match: ENSAMXT00000034831.1 (pep primary_assembly:Astyanax_mexicanus-2.0:3:16211297:16215948:1 gene:ENSAMXG00000034228.1 transcript:ENSAMXT00000034831.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 83.9593 bits (206), Expect = 4.391e-16
Identity = 48/134 (35.82%), Postives = 79/134 (58.96%), Query Frame = 1 
Query: 1321 ENTMPDVWKYFKTLMIPKPDKDDKYNLISSWRPIALLSVVYKIFASILARRLTSWVNRNNLLHIG-QKWG-SVHDGCVEHNSVLSSCLENSKYSKDTPTIIAFLDIKDAFDNVPHDYMWKILRYIGVDEKFTNF 1716
            +  +P  W     ++IPK +KD     I  +RPI+LL+V  KIF S++ARRL +++ RNNL+    QK G +   GC+EHNS++   ++ ++  K    ++ FLD+ +AF +VPH  +WK   +  V E+ +  
Sbjct:  420 KGIIPKEWHRAGGVLIPK-EKDSVA--IDQFRPISLLNVEGKIFFSVVARRLATYLKRNNLIDTSVQKAGIAGFSGCIEHNSMIWHQIQTARAEKKDLHVV-FLDLANAFGSVPHALLWKAFNFFRVPEEISRL 549
[Close]
BLAST of Reverse transcriptase vs. Ensembl Cavefish
Match: ENSAMXT00000032297.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000099.1:149628:151976:-1 gene:ENSAMXG00000039954.1 transcript:ENSAMXT00000032297.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 53.1434 bits (126), Expect = 4.839e-16
Identity = 36/113 (31.86%), Postives = 59/113 (52.21%), Query Frame = 1 
Query: 1360 LMIPKPDKDDKYNLISSWRPIALLSVVYKIFASILARRLTSWVN-RNNLLHIGQKWGSVHDGCVEHNSVLSSCLENSKYSKDTPTIIAFLDIKDAFDNVPHDYMWKILRYIGV 1695
            +++P   K D+  + S++R I LLS+  K+++ +L RR+   V  R      G + G    G ++    LS  LE S +    P  + F+D++ AFD VP   +W +LR  GV
Sbjct:  339 VVVPLFKKGDR-RVCSNYRGITLLSLPGKVYSGVLERRVRRIVEPRIQEEQCGFRPGR---GTLDQLYTLSRVLEGS-WEFAQPVYMCFVDLEKAFDRVPRGVLWGVLREYGV 446

HSP 2 Score: 51.6026 bits (122), Expect = 4.839e-16
Identity = 30/100 (30.00%), Postives = 51/100 (51.00%), Query Frame = 2 
Query: 1709 QTLKLLYTETSSYYTCGPIVTPNIPIKQGVKQGCPLSMILFAIAINTVLQAVTLSKVKPFKIGESSIQVLAYADDIGLIANNTHDLQNILNIAFDVACEI 2008
            + ++ LY    S        + + P++ G++QGCPLS ILF   ++ + +      V+  + G   I  L +ADD+ L+A++  DLQ  L   F   CE+
Sbjct:  452 RAVRSLYDRCQSLVRIAGSKSDSFPVRVGLRQGCPLSPILFITFMDRISRRS--QGVEGIRFGGLGIASLLFADDVVLLASSARDLQLALE-QFAAECEM 548
[Close]
BLAST of Reverse transcriptase vs. Ensembl Cavefish
Match: ENSAMXT00000056440.1 (pep primary_assembly:Astyanax_mexicanus-2.0:19:13299579:13303381:-1 gene:ENSAMXG00000041658.1 transcript:ENSAMXT00000056440.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 62.7734 bits (151), Expect = 1.009e-15
Identity = 43/132 (32.58%), Postives = 65/132 (49.24%), Query Frame = 1 
Query: 1321 ENTMPDVWKYFKTLMIPKPDKDDKYNLISSWRPIALLSVVYKIFASILARRLTSWVNRNNLLHIGQKWGSVHDGCVEHNSVLSSCLENSKYSKDTPTIIAFLDIKDAFDNVPHDYMWKILRYIGVDEKFTNF 1716
            +  +P  W   K L++  P K +  N   +WR I LLSV  K+F  IL  R+ S +++   L   Q       GC++    L + +E S    +TP  I F+D + AFD++  D +WKI+R  GV  K    
Sbjct:  553 KEVIPQDWD--KGLIVKLPKKGNPQN-CDNWRGITLLSVPSKVFCRILLARIESAIDQE--LRQEQAGFRRGRGCIDQIFALRNIIEQS-VEWNTPLHINFIDFRKAFDSLHRDTLWKIVRAYGVPPKLVTL 678

HSP 2 Score: 40.817 bits (94), Expect = 1.009e-15
Identity = 28/97 (28.87%), Postives = 49/97 (50.52%), Query Frame = 2 
Query: 1745 YYTCGPIVTPN----IPIKQGVKQGCPLSMILFAIAINTVLQAVTLSKVKPFKIGE---SSIQVLAYADDIGLIANNTHDLQNILNIAFDVACEIGF 2014
            ++ C  IV+        ++ GV+QGC +S ILF I I+ V++  T    +P  I     S ++ L +ADD+ ++A+    LQ   +     A ++G 
Sbjct:  685 HFECSVIVSGEPSTWFTVESGVRQGCTISPILFLIVIDWVMRNTT---DRPRGIQWTLFSQLEDLDFADDLAVLASKHIHLQEKTDRLNKFAQQVGL 778
[Close]
BLAST of Reverse transcriptase vs. Ensembl Cavefish
Match: ENSAMXT00000048276.1 (pep primary_assembly:Astyanax_mexicanus-2.0:17:7857318:7859867:1 gene:ENSAMXG00000040121.1 transcript:ENSAMXT00000048276.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 55.8398 bits (133), Expect = 4.925e-15
Identity = 29/74 (39.19%), Postives = 45/74 (60.81%), Query Frame = 2 
Query: 1724 LYTETSSYYTCGPIVTPNIPIKQGVKQGCPLSMILFAIAINTVLQAVTLSK-VKPFKIGESSIQVLAYADDIGL 1942
            LYT  S+  + G +++    +++G +QGCPLS  LFA+A+  + QAV  +  +KP  I +S   +  YADDI L
Sbjct:  633 LYTNPSACVSTGHMISAKFLLERGTRQGCPLSPFLFALAVEPLAQAVRQNALIKPISIKDSLHHISLYADDILL 706

HSP 2 Score: 45.8246 bits (107), Expect = 4.925e-15
Identity = 36/116 (31.03%), Postives = 59/116 (50.86%), Query Frame = 1 
Query: 1363 MIPKPDKDDKYNLISSWRPIALLSVVYKIFASILARRLTSWVNRNNLLHIGQKWGSVHDGCVEHN-SVLSSCLENSKYSKDTPTIIAFLDIKDAFDNVPHDYMWKILRYIGVDEKF 1707
            ++ KP KD      SS+RP++LL+   K+FA +LA+RL S +   +L+H  Q  G +       N   L   LE +  S+   + +  LD + AFD +   ++W +L+  G    F
Sbjct:  517 LLLKPGKDSTE--CSSYRPLSLLNTDVKVFAKVLAKRLDSVI--PDLIHSDQT-GFIRSRLASDNLRRLFHILEFAP-SQMLGSAVLSLDAEKAFDRLEWPFLWAVLKRFGFGSAF 626
[Close]
BLAST of Reverse transcriptase vs. Ensembl Medaka
Match: ENSORLT00000040357.1 (pep primary_assembly:ASM223467v1:14:28942701:28945517:1 gene:ENSORLG00000024817.1 transcript:ENSORLT00000040357.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 57.3806 bits (137), Expect = 6.034e-16
Identity = 40/119 (33.61%), Postives = 65/119 (54.62%), Query Frame = 1 
Query: 1363 MIPKPDKDDKYNLISSWRPIALLSVVYKIFASILARRLTSWVNRNNLLHIGQKWGSVHDGCVEHNSVLSSCLENSKYSKDTP--TIIAFLDIKDAFDNVPHDYMWKILRYIGVDEKFTN 1713
            ++PKP KD    LI +WRPI LL   YKI A I A RL S +++  ++   Q    +    + H++ L   L+   Y++  P  ++I +LD   AFD++ H +++  L+  G + KF N
Sbjct:  527 LLPKPGKD--VLLIDNWRPITLLCCDYKILAHIYANRLKSGISQ--IIDESQS-AFIKGRHIHHHTRL--ILDILDYNQIIPQDSLILYLDFFKAFDSLEHAFLFNALKTFGFESKFCN 638

HSP 2 Score: 47.3654 bits (111), Expect = 6.034e-16
Identity = 33/112 (29.46%), Postives = 55/112 (49.11%), Query Frame = 2 
Query: 1715 LKLLYTETSSYYTCGPIVTPNIPIKQGVKQGCPLSMILFAIAINTVLQAVTLSKVKPFK---IGESSIQVLAYADDIGLIANNTHDLQNILN--IAFDVACEIGFEYRPEKC 2035
            +K+ Y + SS        + N P+ +GV+QGCP+S +LF I+    L A+ L     F+   I +   ++  +ADD  L   N + +  ++N  + F  AC  G     +KC
Sbjct:  640 IKMFYKDISSNIAINRGFSNNFPVNRGVRQGCPISPLLFIIS--AELLAIFLKYSPDFEGINIFDREFKISQFADDTALFLKNKNMVPVVINKILLFSRAC--GLTLNLKKC 747
[Close]
BLAST of Reverse transcriptase vs. Ensembl Medaka
Match: ENSORLT00000043450.1 (pep primary_assembly:ASM223467v1:12:7314868:7318593:-1 gene:ENSORLG00000025257.1 transcript:ENSORLT00000043450.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 51.2174 bits (121), Expect = 2.699e-15
Identity = 39/126 (30.95%), Postives = 68/126 (53.97%), Query Frame = 1 
Query: 1363 MIPKPDKDDKYNLISSWRPIALLSVVYKIFASILARRLTSWVNRNNLLHIGQKWGSVHDGCVEHN-SVLSSCLENSKYSKDTPTIIAFLDIKDAFDNVPHDYMWKILRYIGVDEKFTNFETSIH*N 1737
            +IPKP KD     + +WRPI LL+  YKI A ILA R+   +  + ++   Q  G + +  + +N  ++   L+ S+  +D  ++I FLD   AFD++ H+++   L   G  + F N   +++ N
Sbjct:  512 LIPKPRKDPLS--LDNWRPITLLNCDYKILALILANRIKPVL--DTIIDENQS-GFMQNRHISNNIRLILDVLDYSELVQDD-SLILFLDFYKAFDSLEHNFIIAALNKFGFGQFFCNAVQTLYVN 631

HSP 2 Score: 51.2174 bits (121), Expect = 2.699e-15
Identity = 30/114 (26.32%), Postives = 53/114 (46.49%), Query Frame = 2 
Query: 1715 LKLLYTETSSYYTCGPIVTPNIPIKQGVKQGCPLSMILFAIAINTVLQAVTLSKVKPFKIGESSIQVLAYADDIGLIANNTHDLQNILNIAFDVACEIGFEYRPEKCAYI*LPN 2056
            ++ LY  ++S        +P   +K+GV+QGCPLS+ LF +++  +   + LS +K   + E  I +   ADD  L   +   +   +NI    +   G      KC  + + N
Sbjct:  625 VQTLYVNSNSSIKLASGTSPRFFLKRGVRQGCPLSVYLFLLSVQLLNLHIKLSPLKGLIVAEREIFISQLADDTALFLRDASQVAVAINIIQSFSKASGLTLNLNKCEILPVKN 738
[Close]
BLAST of Reverse transcriptase vs. Ensembl Medaka
Match: ENSORLT00000037544.1 (pep primary_assembly:ASM223467v1:22:28708275:28712000:-1 gene:ENSORLG00000028184.1 transcript:ENSORLT00000037544.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 51.2174 bits (121), Expect = 2.835e-15
Identity = 39/126 (30.95%), Postives = 68/126 (53.97%), Query Frame = 1 
Query: 1363 MIPKPDKDDKYNLISSWRPIALLSVVYKIFASILARRLTSWVNRNNLLHIGQKWGSVHDGCVEHN-SVLSSCLENSKYSKDTPTIIAFLDIKDAFDNVPHDYMWKILRYIGVDEKFTNFETSIH*N 1737
            +IPKP KD     + +WRPI LL+  YKI A ILA R+   +  + ++   Q  G + +  + +N  ++   L+ S+  +D  ++I FLD   AFD++ H+++   L   G  + F N   +++ N
Sbjct:  512 LIPKPRKDPLS--LDNWRPITLLNCDYKILALILANRIKPVL--DTIIDENQS-GFMQNRHISNNIRLILDILDYSELVQDD-SLILFLDFYKAFDSLEHNFIIAALNKFGFGQFFCNAVQTLYVN 631

HSP 2 Score: 51.2174 bits (121), Expect = 2.835e-15
Identity = 30/114 (26.32%), Postives = 53/114 (46.49%), Query Frame = 2 
Query: 1715 LKLLYTETSSYYTCGPIVTPNIPIKQGVKQGCPLSMILFAIAINTVLQAVTLSKVKPFKIGESSIQVLAYADDIGLIANNTHDLQNILNIAFDVACEIGFEYRPEKCAYI*LPN 2056
            ++ LY  ++S        +P   +K+GV+QGCPLS+ LF +++  +   + LS +K   + E  I +   ADD  L   +   +   +NI    +   G      KC  + + N
Sbjct:  625 VQTLYVNSNSSIKLASGTSPRFFLKRGVRQGCPLSVYLFLLSVQLLNLHIKLSPLKGLIVAEREIFISQLADDTALFLRDASQVAVAINIIQSFSKASGLTLNLNKCEILPVKN 738
[Close]
BLAST of Reverse transcriptase vs. Ensembl Medaka
Match: ENSORLT00000028663.1 (pep primary_assembly:ASM223467v1:7:26712747:26716472:-1 gene:ENSORLG00000028539.1 transcript:ENSORLT00000028663.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 51.2174 bits (121), Expect = 3.051e-15
Identity = 30/114 (26.32%), Postives = 53/114 (46.49%), Query Frame = 2 
Query: 1715 LKLLYTETSSYYTCGPIVTPNIPIKQGVKQGCPLSMILFAIAINTVLQAVTLSKVKPFKIGESSIQVLAYADDIGLIANNTHDLQNILNIAFDVACEIGFEYRPEKCAYI*LPN 2056
            ++ LY  ++S        +P   +K+GV+QGCPLS+ LF +++  +   + LS +K   + E  I +   ADD  L   +   +   +NI    +   G      KC  + + N
Sbjct:  625 VQTLYVNSNSSIKLASGTSPRFFLKRGVRQGCPLSVYLFLLSVQLLNLHIKLSPLKGLIVAEREIFISQLADDTALFLRDASQVAVAINIIQSFSKASGLTLNLNKCEILPVKN 738

HSP 2 Score: 50.8322 bits (120), Expect = 3.051e-15
Identity = 39/126 (30.95%), Postives = 68/126 (53.97%), Query Frame = 1 
Query: 1363 MIPKPDKDDKYNLISSWRPIALLSVVYKIFASILARRLTSWVNRNNLLHIGQKWGSVHDGCVEHN-SVLSSCLENSKYSKDTPTIIAFLDIKDAFDNVPHDYMWKILRYIGVDEKFTNFETSIH*N 1737
            +IPKP KD     + +WRPI LL+  YKI A ILA R+   +  + ++   Q  G + +  + +N  ++   L+ S+  +D  ++I FLD   AFD++ H+++   L   G  + F N   +++ N
Sbjct:  512 LIPKPRKDPLS--LDNWRPITLLNCDYKILALILANRIKPVL--DTIIDENQS-GFMQNRHISNNIRLILDILDYSELVQDD-SLILFLDFYKAFDSLEHNFIIAALNKFGFGQFFCNAVQTLYVN 631
[Close]
BLAST of Reverse transcriptase vs. Ensembl Medaka
Match: ENSORLT00000043337.1 (pep primary_assembly:ASM223467v1:9:26106542:26110291:1 gene:ENSORLG00000021884.1 transcript:ENSORLT00000043337.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 58.9214 bits (141), Expect = 3.505e-15
Identity = 41/119 (34.45%), Postives = 65/119 (54.62%), Query Frame = 1 
Query: 1363 MIPKPDKDDKYNLISSWRPIALLSVVYKIFASILARRLTSWVNRNNLLHIGQKWGSVHDGCVEHNSVLSSCLENSKYSKDTP--TIIAFLDIKDAFDNVPHDYMWKILRYIGVDEKFTN 1713
            ++PKP KD    LI +WRPI LL   YKI A I A RL S +++  ++   Q    +    + H++ L   L+   Y++  P  ++I FLD   AFD++ H +++  L+  G + KF N
Sbjct:  526 LLPKPGKD--VLLIDNWRPITLLCCDYKILAHIYANRLKSGISQ--IIDESQS-AFIKGRHIHHHTRL--ILDILDYNQIIPQDSLILFLDFFKAFDSLEHAFLFNALKTFGFESKFCN 637

HSP 2 Score: 43.1282 bits (100), Expect = 3.505e-15
Identity = 29/110 (26.36%), Postives = 52/110 (47.27%), Query Frame = 2 
Query: 1715 LKLLYTETSSYYTCGPIVTPNIPIKQGVKQGCPLSMILFAIAINTVLQAVTLSKVKPFK---IGESSIQVLAYADDIGLIANNTHDLQNILNIAFDVACEIGFEYRPEKC 2035
            +K+ Y + SS        + + P+ +GV+QGCP+S +LF I+    L A+ L     F+   I +   ++  +ADD  L   N + +  ++N     +   G     +KC
Sbjct:  639 IKMFYKDISSNIALNRGFSNSFPVNRGVRQGCPISPLLFIIS--AELLAIFLKYSPDFEGINIFDREFKISQFADDTALFLKNKNIVPVVINKILLFSRASGLTLNLKKC 746
[Close]
BLAST of Reverse transcriptase vs. Planmine SMEST
Match: SMESG000079542.1 (SMESG000079542.1)

HSP 1 Score: 402.905 bits (1034), Expect = 0.000e+0
Identity = 242/366 (66.12%), Postives = 271/366 (74.04%), Query Frame = 3 
Query:  102 MFSDDPIHHATTFILFLEEFISGNVVNRDKVIEKLVNMQSKMCNEVVVNLNLINCKITEXXXXXXXXXXXXSKNFIFFKQYNDHSNTG*LHSMLK*IFKY*KFNESKNCVYHYCSSLSSANFDGHRLQL**TLST*SFILL------PNFPYLCTANSLLTK---IKETPRLCKILGNCPHDIRGSGFVSKILICAFIKHNALNLSLENVNLRDIGDLIKSLLSTVQDIIKNSIKNLPDNKTNVKQNLDQRILLINENLLTLRNASVNEISNLIINEIRIRKETNVTKISRPYLGNKISIVPPPKTNFKSSFNQNMKTTVYNILVNNEIKERPTIKDHISNFSHQDQVIKSYDNVLNFGLKSQNSF 1172
            MFSDDPIHHA+TFILFLEEFISGNVVNRDKVIEKLVNMQSKMC+EVVVNLNL NCKITE +LTNFFINLNLS+NFIF    N+   T  L+   +      +F   +N +         A     ++ +    ++  + LL      PNFPYLCTANSLLTK   IKETP LCK+LGNCPHDIRGSG VS+ILICAFIKH AL+LSLEN+NLRDIG++IKSLL TVQDIIK+SIKNL DNKTNVK  LDQRILLINENLLTL+NA+VNE                        + NKIS VP PKT FKSSFNQNMKTTVY ILVN+EI ERPTIKDHI NFSHQD +IKSYDNVLNFGLKSQN+ 
Sbjct:    1 MFSDDPIHHASTFILFLEEFISGNVVNRDKVIEKLVNMQSKMCDEVVVNLNLKNCKITEKLLTNFFINLNLSENFIFL---NNTMTTAILNDCTQ---YLNEFLNIENLMKAKIVFAIIAPPKARQILMIIDYTSDEYYLLNPSSLLPNFPYLCTANSLLTKINEIKETPELCKLLGNCPHDIRGSGLVSRILICAFIKHYALDLSLENINLRDIGNVIKSLLPTVQDIIKDSIKNLSDNKTNVKLKLDQRILLINENLLTLQNANVNE------------------------MRNKISKVPHPKTKFKSSFNQNMKTTVYKILVNDEINERPTIKDHIGNFSHQDPLIKSYDNVLNFGLKSQNTL 336

HSP 2 Score: 253.832 bits (647), Expect = 0.000e+0
Identity = 120/131 (91.60%), Postives = 124/131 (94.66%), Query Frame = 1 
Query: 1321 ENTMPDVWKYFKTLMIPKPDKDDKYNLISSWRPIALLSVVYKIFASILARRLTSWVNRNNLLHIGQKWGSVHDGCVEHNSVLSSCLENSKYSKDTPTIIAFLDIKDAFDNVPHDYMWKILRYIGVDEKFTN 1713
            ENTMPDVWK FKTLM PKPDK DKYNLISSWRPIALLSVVYKIFASIL+RRLTSWVNRNNLLHIGQK GSVHDGCVEHNSVLSSCLE+SKYSKDTP IIAFLDIKDAF +VPHDYMWKILR+IGV EKFTN
Sbjct:  388 ENTMPDVWKSFKTLMTPKPDKGDKYNLISSWRPIALLSVVYKIFASILSRRLTSWVNRNNLLHIGQKGGSVHDGCVEHNSVLSSCLEHSKYSKDTPIIIAFLDIKDAFGSVPHDYMWKILRHIGVGEKFTN 518

HSP 3 Score: 233.417 bits (594), Expect = 0.000e+0
Identity = 114/125 (91.20%), Postives = 118/125 (94.40%), Query Frame = 2 
Query: 1700 KNSQTLKLLYTETSSYYTCGPIVTPNIPIKQGVKQGCPLSMILFAIAINTVLQAVTLSKVKPFKIGESSIQVLAYADDIGLIANNTHDLQNILNIAFDVACEIGFEYRPEKCAYI*LPNVDTIGE 2074
            K + TLKLLYTETSSYYTCGPIVTPNIPIKQGVKQGCPLSMILFAIAIN VLQAVTLSKVKPFK+GESSIQVLAYADDI LIANNTHDLQNILNIAFDVA EIGFEYRP+KCAYI LPNV+TI E
Sbjct:  515 KFTNTLKLLYTETSSYYTCGPIVTPNIPIKQGVKQGCPLSMILFAIAINPVLQAVTLSKVKPFKVGESSIQVLAYADDIALIANNTHDLQNILNIAFDVAREIGFEYRPKKCAYIQLPNVETISE 639

HSP 4 Score: 99.7525 bits (247), Expect = 0.000e+0
Identity = 47/57 (82.46%), Postives = 52/57 (91.23%), Query Frame = 1 
Query: 2146 RLVNAAKKLMNSDLCGWQKLKPYKTFLHSRLTFPFRTIEIKISALSANVCNNTTKLR 2316
            ++V+ AKKL +SDLCGWQKLK YKTFLHSRLTFPFRT + KISALSANVCNNTTKLR
Sbjct:  651 KVVSDAKKLADSDLCGWQKLKAYKTFLHSRLTFPFRTRDFKISALSANVCNNTTKLR 707

HSP 5 Score: 82.4185 bits (202), Expect = 0.000e+0
Identity = 40/44 (90.91%), Postives = 41/44 (93.18%), Query Frame = 2 
Query: 1193 EVIFELKRADNTSPGIDGIKYYDIAIFDPEGKLLTLLYNKIITK 1324
            EVIFELKRADNTSPGIDGIKY DIAI D EGKLLTLLYNKIIT+
Sbjct:  345 EVIFELKRADNTSPGIDGIKYNDIAIHDAEGKLLTLLYNKIITE 388

HSP 6 Score: 110.153 bits (274), Expect = 2.012e-24
Identity = 53/62 (85.48%), Postives = 57/62 (91.94%), Query Frame = 1 
Query:  331 FLNNTMTTAILDDCTQCLNEFLNIENLMKAKIVFTIIAPP*AQQILMVTDYNSDEHYLLNPS 516
            FLNNTMTTAIL+DCTQ LNEFLNIENLMKAKIVF IIAPP A+QILM+ DY SDE+YLLNPS
Sbjct:   77 FLNNTMTTAILNDCTQYLNEFLNIENLMKAKIVFAIIAPPKARQILMIIDYTSDEYYLLNPS 138
[Close]
BLAST of Reverse transcriptase vs. Planmine SMEST
Match: SMESG000047025.1 (SMESG000047025.1)

HSP 1 Score: 339.347 bits (869), Expect = 0.000e+0
Identity = 182/213 (85.45%), Postives = 197/213 (92.49%), Query Frame = 3 
Query:  519 LLPNFPYLCTANSLLTKI---KETPRLCKILGNCPHDIRGSGFVSKILICAFIKHNALNLSLENVNLRDIGDLIKSLLSTVQDIIKNSIKNLPDNKTNVKQNLDQRILLINENLLTLRNASVNEISNLIINEIRIRKETNVTKISRPYLGNKISIVPPPKTNFKSSFNQNMKTTVYNILVNNEIKERPTIKDHISNFSHQDQVIKSYDNVLNF 1148
            LLPNFPYLCTANSLLTKI   KETP LCK+LGNCP+DIRGSG VS+ILICAFIKH AL+LSLEN+NLRDIG++IKSLL TVQDIIK+SIKNLPDNKTNVK  LDQ ILLINENLLTL+NASVNEISN IINEIRIRK+T+VTKIS PYLGNKIS VPPPKTN+KSSFNQN+KTTVYNILVN EI ERPTIKDHISNFSHQD +IKSYDNV+NF
Sbjct:   18 LLPNFPYLCTANSLLTKINEIKETPELCKLLGNCPYDIRGSGLVSRILICAFIKHYALDLSLENINLRDIGNVIKSLLPTVQDIIKDSIKNLPDNKTNVKLKLDQLILLINENLLTLQNASVNEISNFIINEIRIRKKTSVTKISGPYLGNKISKVPPPKTNYKSSFNQNVKTTVYNILVNVEINERPTIKDHISNFSHQDPLIKSYDNVINF 230

HSP 2 Score: 187.578 bits (475), Expect = 0.000e+0
Identity = 94/103 (91.26%), Postives = 95/103 (92.23%), Query Frame = 2 
Query: 1790 QGVKQGCPLSMILFAIAINTVLQAVTLSKVKPFKIGESSIQVLAYADDIGLIANNTHDLQNILNIAFDVACEIGFEYRPEKCAYI*LPNVDTIGEISINNIQI 2098
            Q VKQGCPLSMILFAIAIN VLQAVTLSKVKPFKIGESSIQVLAYADDI LIANNTHDLQNILNIAFDV  EIGFEYR EKCAYI LPNVDTI EISINNI+I
Sbjct:  378 QDVKQGCPLSMILFAIAINPVLQAVTLSKVKPFKIGESSIQVLAYADDIALIANNTHDLQNILNIAFDVTREIGFEYRHEKCAYIQLPNVDTISEISINNIKI 480

HSP 3 Score: 172.555 bits (436), Expect = 0.000e+0
Identity = 86/125 (68.80%), Postives = 90/125 (72.00%), Query Frame = 1 
Query: 1321 ENTMPDVWKYFKTLMIPKPDKDDKYNLISSWRPIALLSVVYKIFASILARRLTSWVNRNNLLHIGQKWGSVHDGCVEHNSVLSSCLENSKYSKDTPTIIAFLDIKDAFDNVPHDYMWKILRYIGV 1695
            ENTMP VWK FKTLMIPKPDK                           ARRLTSWVNRNNLLH GQK GSVHDGCVEHN VLSSCLE+SKYSKDTP IIAFLDIKDAF +VPHDYM KIL++IGV
Sbjct:  276 ENTMPGVWKSFKTLMIPKPDK---------------------------ARRLTSWVNRNNLLHFGQKGGSVHDGCVEHNPVLSSCLEHSKYSKDTPIIIAFLDIKDAFGSVPHDYMRKILQHIGV 373

HSP 4 Score: 86.2705 bits (212), Expect = 0.000e+0
Identity = 41/44 (93.18%), Postives = 42/44 (95.45%), Query Frame = 2 
Query: 1193 EVIFELKRADNTSPGIDGIKYYDIAIFDPEGKLLTLLYNKIITK 1324
            EVIFELKRADNTSPGIDGIKY DIAI DPEGKLLTLLYNKIIT+
Sbjct:  233 EVIFELKRADNTSPGIDGIKYNDIAILDPEGKLLTLLYNKIITE 276

HSP 5 Score: 35.8094 bits (81), Expect = 0.000e+0
Identity = 14/16 (87.50%), Postives = 15/16 (93.75%), Query Frame = 1 
Query:  469 MVTDYNSDEHYLLNPS 516
            M+ DYNSDEHYLLNPS
Sbjct:    1 MIIDYNSDEHYLLNPS 16
[Close]
BLAST of Reverse transcriptase vs. Planmine SMEST
Match: SMESG000021486.1 (SMESG000021486.1)

HSP 1 Score: 201.445 bits (511), Expect = 1.102e-156
Identity = 92/127 (72.44%), Postives = 108/127 (85.04%), Query Frame = 1 
Query: 1330 MPDVWKYFKTLMIPKPDKDDKYNLISSWRPIALLSVVYKIFASILARRLTSWVNRNNLLHIGQKWGSVHDGCVEHNSVLSSCLENSKYSKDTPTIIAFLDIKDAFDNVPHDYMWKILRYIGVDEKFT 1710
            +PD WK FKTL+ PKP+KD KY+ +SSWRPIALLSV+YK+FAS LA RLT W+N NNLLHIGQK GS HDGCVEHNS+LS+ LE+SKYSK+ P  IA+LDIKDAF +VPHDYMW +LR+IGV E FT
Sbjct: 3106 IPDSWKSFKTLLTPKPNKDGKYDDVSSWRPIALLSVIYKVFASCLASRLTYWINTNNLLHIGQKGGSRHDGCVEHNSILSAALEHSKYSKNCPLAIAWLDIKDAFGSVPHDYMWSLLRFIGVGEDFT 3232

HSP 2 Score: 181.03 bits (458), Expect = 1.102e-156
Identity = 85/128 (66.41%), Postives = 103/128 (80.47%), Query Frame = 2 
Query: 1715 LKLLYTETSSYYTCGPIVTPNIPIKQGVKQGCPLSMILFAIAINTVLQAVTLSKVKPFKIGESSIQVLAYADDIGLIANNTHDLQNILNIAFDVACEIGFEYRPEKCAYI*LPNVDTIGEISINNIQI 2098
            L+LLY++TS++Y+CGPI+TPNIPIKQGVKQGCP+SMILFA+AIN VL+AV+ S  +PF IGES +++LAYADDI LIA +  DLQ I NIA   A EIGFEYRPEKC Y+ LP V    EI INN +I
Sbjct: 3235 LQLLYSDTSTFYSCGPILTPNIPIKQGVKQGCPISMILFALAINPVLEAVSRSDCEPFMIGESPVKILAYADDIALIAKSVEDLQKITNIAVRTAAEIGFEYRPEKCGYLQLPKVHIDSEILINNTKI 3362

HSP 3 Score: 117.087 bits (292), Expect = 1.102e-156
Identity = 135/413 (32.69%), Postives = 202/413 (48.91%), Query Frame = 3 
Query:    3 AVG-NRREYNRLLE*IYS*KTIIFKKSHTLFRFVMFSDDPIHHATTFILFLEEFISGNVVNRDKVIEKLVNMQSKMCNEVVVNLNLINCKITEXXXXXXXXXXXXSKNFIFFKQYNDHSNTG*LHSMLK*IFKY*KFNE--SKNCVYHYCSSLSSANFDGHR--LQL**TLST*SFILLPNFPYLCTANS-------LLTKIKE---TPRLCKILGNCPHDIRGSGFVSKILICAFIKHNALNLSLENVNLRDIGDLIKSLLSTVQDIIKNSIKNLPDNKTNVKQ------NLDQRILLINENLLTLRNASVNEISNLIINEIRIRKETNVTKISRPYLGNKISIVPPPKTN---FKSSFNQNMKTTVYNILVNNEIKERPTIKDHISNFSHQDQVIKSYDNVLNFGLKSQNS 1169
            A+G N  EY  L + I S + +IF   H +    + SDD +  A+ FI+ +E  I    V+  +  E L +++ K  NE+ +NLN INCKI E I   +F NL L+ NF+     N+   T  L    + + K+  + +      ++   +         HR  L +   ++   +I+ P    LC   S          KI E   TP     +G+ P+DIRGSG VSK+LICAFIK+ A +L L N+NLR+IG  I SLL     II  S K L  + T + +      NL+QR L I+E L  + + +VNEI ++I+ +   R    V    +PYLGNK     P K N       F  +MK TV  +  N  +  RPT +  + NF  +     +++N LNF  +S+ +
Sbjct: 2662 AIGENMTEY--LNKFIISNRKVIFISEHNIEHASITSDDSLLFASAFIILIESLIFSKQVSHTRTSEFLKSVKIKQANEICLNLNSINCKINEEITAKYFSNLKLNSNFMLL---NETMCTAILDDCTRHLTKFLNYEDLIKAQVIFAIIAP------PKHREILLIIDYITDEYYIINPT--TLCGTESSQSVCRIFRNKINEIKETPGRVINMGDIPNDIRGSGLVSKLLICAFIKNYANDLPLINLNLREIGTNISSLLP----IISESKKILMSDLTKITKIKKSNLNLEQRKLKIDELLHDVSHLTVNEIIDIIVRQFPDR----VKSKHKPYLGNKTR---PQKINKGILVRKFMTDMKWTVDKVFNNTNVSIRPTFQKIVLNFLIKSPFQGNWNNPLNFCKRSKQA 3050

HSP 4 Score: 73.559 bits (179), Expect = 1.102e-156
Identity = 31/43 (72.09%), Postives = 38/43 (88.37%), Query Frame = 2 
Query: 1190 IEVIFELKRADNTSPGIDGIKYYDIAIFDPEGKLLTLLYNKII 1318
             E++F+LKRADNTSPGIDGI+Y D+ + DPEGKLLT L+NKII
Sbjct: 3059 FEIMFQLKRADNTSPGIDGIQYRDLLLLDPEGKLLTFLFNKII 3101

HSP 5 Score: 66.6254 bits (161), Expect = 1.102e-156
Identity = 32/52 (61.54%), Postives = 38/52 (73.08%), Query Frame = 1 
Query: 2146 RLVNAAKKLMNSDLCGWQKLKPYKTFLHSRLTFPFRTIEIKISALSANVCNN 2301
            + V+  +KL NS+L GWQKLK YK F+HSRL FPFRT EIK  ALS +  NN
Sbjct: 3392 KAVSDTRKLANSELLGWQKLKAYKIFVHSRLIFPFRTREIKTGALSKSNGNN 3443

HSP 6 Score: 201.445 bits (511), Expect = 1.196e-156
Identity = 92/127 (72.44%), Postives = 108/127 (85.04%), Query Frame = 1 
Query: 1330 MPDVWKYFKTLMIPKPDKDDKYNLISSWRPIALLSVVYKIFASILARRLTSWVNRNNLLHIGQKWGSVHDGCVEHNSVLSSCLENSKYSKDTPTIIAFLDIKDAFDNVPHDYMWKILRYIGVDEKFT 1710
            +PD WK FKTL+ PKP+KD KY+ +SSWRPIALLSV+YK+FAS LA RLT W+N NNLLHIGQK GS HDGCVEHNS+LS+ LE+SKYSK+ P  IA+LDIKDAF +VPHDYMW +LR+IGV E FT
Sbjct: 5462 IPDSWKSFKTLLTPKPNKDGKYDDVSSWRPIALLSVIYKVFASCLASRLTYWINTNNLLHIGQKGGSRHDGCVEHNSILSAALEHSKYSKNCPLAIAWLDIKDAFGSVPHDYMWSLLRFIGVGEDFT 5588

HSP 7 Score: 181.03 bits (458), Expect = 1.196e-156
Identity = 85/128 (66.41%), Postives = 103/128 (80.47%), Query Frame = 2 
Query: 1715 LKLLYTETSSYYTCGPIVTPNIPIKQGVKQGCPLSMILFAIAINTVLQAVTLSKVKPFKIGESSIQVLAYADDIGLIANNTHDLQNILNIAFDVACEIGFEYRPEKCAYI*LPNVDTIGEISINNIQI 2098
            L+LLY++TS++Y+CGPI+TPNIPIKQGVKQGCP+SMILFA+AIN VL+AV+ S  +PF IGES +++LAYADDI LIA +  DLQ I NIA   A EIGFEYRPEKC Y+ LP V    EI INN +I
Sbjct: 5591 LQLLYSDTSTFYSCGPILTPNIPIKQGVKQGCPISMILFALAINPVLEAVSRSDCEPFMIGESPVKILAYADDIALIAKSVEDLQKITNIAVRTAAEIGFEYRPEKCGYLQLPKVHIDSEILINNTKI 5718

HSP 8 Score: 117.087 bits (292), Expect = 1.196e-156
Identity = 135/413 (32.69%), Postives = 202/413 (48.91%), Query Frame = 3 
Query:    3 AVG-NRREYNRLLE*IYS*KTIIFKKSHTLFRFVMFSDDPIHHATTFILFLEEFISGNVVNRDKVIEKLVNMQSKMCNEVVVNLNLINCKITEXXXXXXXXXXXXSKNFIFFKQYNDHSNTG*LHSMLK*IFKY*KFNE--SKNCVYHYCSSLSSANFDGHR--LQL**TLST*SFILLPNFPYLCTANS-------LLTKIKE---TPRLCKILGNCPHDIRGSGFVSKILICAFIKHNALNLSLENVNLRDIGDLIKSLLSTVQDIIKNSIKNLPDNKTNVKQ------NLDQRILLINENLLTLRNASVNEISNLIINEIRIRKETNVTKISRPYLGNKISIVPPPKTN---FKSSFNQNMKTTVYNILVNNEIKERPTIKDHISNFSHQDQVIKSYDNVLNFGLKSQNS 1169
            A+G N  EY  L + I S + +IF   H +    + SDD +  A+ FI+ +E  I    V+  +  E L +++ K  NE+ +NLN INCKI E I   +F NL L+ NF+     N+   T  L    + + K+  + +      ++   +         HR  L +   ++   +I+ P    LC   S          KI E   TP     +G+ P+DIRGSG VSK+LICAFIK+ A +L L N+NLR+IG  I SLL     II  S K L  + T + +      NL+QR L I+E L  + + +VNEI ++I+ +   R    V    +PYLGNK     P K N       F  +MK TV  +  N  +  RPT +  + NF  +     +++N LNF  +S+ +
Sbjct: 5018 AIGENMTEY--LNKFIISNRKVIFISEHNIEHASITSDDSLLFASAFIILIESLIFSKQVSHTRTSEFLKSVKIKQANEICLNLNSINCKINEEITAKYFSNLKLNSNFMLL---NETMCTAILDDCTRHLTKFLNYEDLIKAQVIFAIIAP------PKHREILLIIDYITDEYYIINPT--TLCGTESSQSVCRIFRNKINEIKETPGRVINMGDIPNDIRGSGLVSKLLICAFIKNYANDLPLINLNLREIGTNISSLLP----IISESKKILMSDLTKITKIKKSNLNLEQRKLKIDELLHDVSHLTVNEIIDIIVRQFPDR----VKSKHKPYLGNKTR---PQKINKGILVRKFMTDMKWTVDKVFNNTNVSIRPTFQKIVLNFLIKSPFHGNWNNPLNFCKRSKQA 5406

HSP 9 Score: 73.559 bits (179), Expect = 1.196e-156
Identity = 31/43 (72.09%), Postives = 38/43 (88.37%), Query Frame = 2 
Query: 1190 IEVIFELKRADNTSPGIDGIKYYDIAIFDPEGKLLTLLYNKII 1318
             E++F+LKRADNTSPGIDGI+Y D+ + DPEGKLLT L+NKII
Sbjct: 5415 FEIMFQLKRADNTSPGIDGIQYRDLLLLDPEGKLLTFLFNKII 5457

HSP 10 Score: 66.6254 bits (161), Expect = 1.196e-156
Identity = 32/52 (61.54%), Postives = 38/52 (73.08%), Query Frame = 1 
Query: 2146 RLVNAAKKLMNSDLCGWQKLKPYKTFLHSRLTFPFRTIEIKISALSANVCNN 2301
            + V+  +KL NS+L GWQKLK YK F+HSRL FPFRT EIK  ALS +  NN
Sbjct: 5748 KAVSDTRKLANSELLGWQKLKAYKIFVHSRLIFPFRTREIKTGALSKSNGNN 5799

HSP 11 Score: 176.022 bits (445), Expect = 5.516e-57
Identity = 83/128 (64.84%), Postives = 103/128 (80.47%), Query Frame = 2 
Query: 1715 LKLLYTETSSYYTCGPIVTPNIPIKQGVKQGCPLSMILFAIAINTVLQAVTLSKVKPFKIGESSIQVLAYADDIGLIANNTHDLQNILNIAFDVACEIGFEYRPEKCAYI*LPNVDTIGEISINNIQI 2098
            L+LLYT+TSS+Y C  + TP+IPIK+GVKQGCP+SMILFAIAIN VL+A++LS   PF IG SS+QVLAYADDI LIA N+ DLQ + ++A + A  IGFEYRPEKCAYI  P V+T+ EI +NN+ I
Sbjct:  916 LQLLYTDTSSFYQCNQMQTPDIPIKKGVKQGCPISMILFAIAINPVLEAISLSNTTPFMIGTSSVQVLAYADDIALIATNSSDLQTLFDLAVNTANMIGFEYRPEKCAYIQYPKVETVSEIIVNNVLI 1043

HSP 12 Score: 64.3142 bits (155), Expect = 5.516e-57
Identity = 35/64 (54.69%), Postives = 45/64 (70.31%), Query Frame = 1 
Query: 2146 RLVNAAKKLMNSDLCGWQKLKPYKTFLHSRLTFPFRTIEIKISALS-------ANVCNNTTKLR 2316
            +++   KK+ +S+L GWQKLK YK FLHSRL FPFRT EIK S+L+        NV NNT++LR
Sbjct: 1073 KVIEDTKKIAHSNLYGWQKLKAYKIFLHSRLIFPFRTREIKTSSLADPSRNSRINV-NNTSQLR 1135

HSP 13 Score: 22.3274 bits (46), Expect = 5.516e-57
Identity = 8/11 (72.73%), Postives = 11/11 (100.00%), Query Frame = 3 
Query: 2118 NQSPYEILNKV 2150
            +QSPYEI++KV
Sbjct: 1064 DQSPYEIVSKV 1074

HSP 14 Score: 122.479 bits (306), Expect = 1.932e-51
Identity = 77/185 (41.62%), Postives = 108/185 (58.38%), Query Frame = 3 
Query:  603 GNCPHDIRGSGFVSKILICAFIKHNALNLSLENVNLRDIGDLIKSLLSTV--QDIIKNSIKNLPDNKTNVKQNLDQRILLINENLLTLRNASVNEISNLIINEIRIRKETNVTKISRPYLGNKISIVPPPKTNFKSSFNQNMKTTVYNILVNNEIKERPTIKDHISNFSHQDQVIKSYDNVLNFG 1151
            G+C HDIRGSG +SKILICAFI++ AL+ SLEN+NL +IG ++ SL+  V   D +K   +        +K NL QR   IN+ L+ L ++ V+EI   I+  I   +     +   PYLG   +   P K N  + F  NMKTTVY IL +  +K  P  ++ I+NF+H D V + +DNVL F 
Sbjct:  695 GHCRHDIRGSGLLSKILICAFIRNYALDYSLENINLHEIGKIVNSLVPIVDGSDNVKYEKREKLKAANTLKLNLKQRKEKINQLLIKLSDSDVDEIICTILTNIPRLENLQKKEFREPYLGKNHN--KPKKFNNPAEFLTNMKTTVYRILNDQSVKIEPPFQEIINNFTHPDVVPECWDNVLKFS 877

HSP 15 Score: 75.485 bits (184), Expect = 1.932e-51
Identity = 32/61 (52.46%), Postives = 46/61 (75.41%), Query Frame = 1 
Query:  331 FLNNTMTTAILDDCTQCLNEFLNIENLMKAKIVFTIIAPP*AQQILMVTDYNSDEHYLLNP 513
             LN T+  AI DDCTQ + +FLNIE +MKA ++F II+PP   ++L+V DY+++EHY L+P
Sbjct:  601 LLNKTLCVAISDDCTQYMKDFLNIECMMKANVIFAIISPPKVHELLLVIDYSTEEHYYLSP 661

HSP 16 Score: 38.891 bits (89), Expect = 1.932e-51
Identity = 22/74 (29.73%), Postives = 40/74 (54.05%), Query Frame = 3 
Query:   57 KTIIFKKSHTLFRFVMFSDDPIHHATTFILFLEEFISGNVVNRDKVIEKLVNMQSKMCNEVVVNLNLINCKITE 278
            + + F  +H +      S D  + A+ FI+  E+ +    ++ DKV + L  ++++ C E+   LNLIN KIT+
Sbjct:  510 RNVKFLANHHISHSTFLSYDTQYFASHFIVLFEKLVYEIHLDCDKVYDSLSKIKTETCIEISPGLNLINGKITD 583

HSP 17 Score: 27.335 bits (59), Expect = 1.932e-51
Identity = 11/22 (50.00%), Postives = 16/22 (72.73%), Query Frame = 1 
Query:    1 KLLGIGENITDYLNKFILEKQL 66
            +L+ IG  ITDYLN+F L + +
Sbjct:  491 RLIEIGVKITDYLNQFCLNRNV 512

HSP 18 Score: 80.8777 bits (198), Expect = 3.663e-15
Identity = 35/63 (55.56%), Postives = 51/63 (80.95%), Query Frame = 1 
Query:  328 FFLNNTMTTAILDDCTQCLNEFLNIENLMKAKIVFTIIAPP*AQQILMVTDYNSDEHYLLNPS 516
              LN TM TAILDDCT+ L +FLN E+L+KA+++F IIAPP  ++IL++ DY +DE+Y++NP+
Sbjct: 2769 MLLNETMCTAILDDCTRHLTKFLNYEDLIKAQVIFAIIAPPKHREILLIIDYITDEYYIINPT 2831

HSP 19 Score: 80.8777 bits (198), Expect = 3.663e-15
Identity = 35/63 (55.56%), Postives = 51/63 (80.95%), Query Frame = 1 
Query:  328 FFLNNTMTTAILDDCTQCLNEFLNIENLMKAKIVFTIIAPP*AQQILMVTDYNSDEHYLLNPS 516
              LN TM TAILDDCT+ L +FLN E+L+KA+++F IIAPP  ++IL++ DY +DE+Y++NP+
Sbjct: 5125 MLLNETMCTAILDDCTRHLTKFLNYEDLIKAQVIFAIIAPPKHREILLIIDYITDEYYIINPT 5187
[Close]
BLAST of Reverse transcriptase vs. Planmine SMEST
Match: SMESG000021486.1 (SMESG000021486.1)

HSP 1 Score: 201.445 bits (511), Expect = 1.388e-156
Identity = 92/127 (72.44%), Postives = 108/127 (85.04%), Query Frame = 1 
Query: 1330 MPDVWKYFKTLMIPKPDKDDKYNLISSWRPIALLSVVYKIFASILARRLTSWVNRNNLLHIGQKWGSVHDGCVEHNSVLSSCLENSKYSKDTPTIIAFLDIKDAFDNVPHDYMWKILRYIGVDEKFT 1710
            +PD WK FKTL+ PKP+KD KY+ +SSWRPIALLSV+YK+FAS LA RLT W+N NNLLHIGQK GS HDGCVEHNS+LS+ LE+SKYSK+ P  IA+LDIKDAF +VPHDYMW +LR+IGV E FT
Sbjct: 3077 IPDSWKSFKTLLTPKPNKDGKYDDVSSWRPIALLSVIYKVFASCLASRLTYWINTNNLLHIGQKGGSRHDGCVEHNSILSAALEHSKYSKNCPLAIAWLDIKDAFGSVPHDYMWSLLRFIGVGEDFT 3203

HSP 2 Score: 181.03 bits (458), Expect = 1.388e-156
Identity = 85/128 (66.41%), Postives = 103/128 (80.47%), Query Frame = 2 
Query: 1715 LKLLYTETSSYYTCGPIVTPNIPIKQGVKQGCPLSMILFAIAINTVLQAVTLSKVKPFKIGESSIQVLAYADDIGLIANNTHDLQNILNIAFDVACEIGFEYRPEKCAYI*LPNVDTIGEISINNIQI 2098
            L+LLY++TS++Y+CGPI+TPNIPIKQGVKQGCP+SMILFA+AIN VL+AV+ S  +PF IGES +++LAYADDI LIA +  DLQ I NIA   A EIGFEYRPEKC Y+ LP V    EI INN +I
Sbjct: 3206 LQLLYSDTSTFYSCGPILTPNIPIKQGVKQGCPISMILFALAINPVLEAVSRSDCEPFMIGESPVKILAYADDIALIAKSVEDLQKITNIAVRTAAEIGFEYRPEKCGYLQLPKVHIDSEILINNTKI 3333

HSP 3 Score: 117.087 bits (292), Expect = 1.388e-156
Identity = 135/413 (32.69%), Postives = 202/413 (48.91%), Query Frame = 3 
Query:    3 AVG-NRREYNRLLE*IYS*KTIIFKKSHTLFRFVMFSDDPIHHATTFILFLEEFISGNVVNRDKVIEKLVNMQSKMCNEVVVNLNLINCKITEXXXXXXXXXXXXSKNFIFFKQYNDHSNTG*LHSMLK*IFKY*KFNE--SKNCVYHYCSSLSSANFDGHR--LQL**TLST*SFILLPNFPYLCTANS-------LLTKIKE---TPRLCKILGNCPHDIRGSGFVSKILICAFIKHNALNLSLENVNLRDIGDLIKSLLSTVQDIIKNSIKNLPDNKTNVKQ------NLDQRILLINENLLTLRNASVNEISNLIINEIRIRKETNVTKISRPYLGNKISIVPPPKTN---FKSSFNQNMKTTVYNILVNNEIKERPTIKDHISNFSHQDQVIKSYDNVLNFGLKSQNS 1169
            A+G N  EY  L + I S + +IF   H +    + SDD +  A+ FI+ +E  I    V+  +  E L +++ K  NE+ +NLN INCKI E I   +F NL L+ NF+     N+   T  L    + + K+  + +      ++   +         HR  L +   ++   +I+ P    LC   S          KI E   TP     +G+ P+DIRGSG VSK+LICAFIK+ A +L L N+NLR+IG  I SLL     II  S K L  + T + +      NL+QR L I+E L  + + +VNEI ++I+ +   R    V    +PYLGNK     P K N       F  +MK TV  +  N  +  RPT +  + NF  +     +++N LNF  +S+ +
Sbjct: 2633 AIGENMTEY--LNKFIISNRKVIFISEHNIEHASITSDDSLLFASAFIILIESLIFSKQVSHTRTSEFLKSVKIKQANEICLNLNSINCKINEEITAKYFSNLKLNSNFMLL---NETMCTAILDDCTRHLTKFLNYEDLIKAQVIFAIIAP------PKHREILLIIDYITDEYYIINPT--TLCGTESSQSVCRIFRNKINEIKETPGRVINMGDIPNDIRGSGLVSKLLICAFIKNYANDLPLINLNLREIGTNISSLLP----IISESKKILMSDLTKITKIKKSNLNLEQRKLKIDELLHDVSHLTVNEIIDIIVRQFPDR----VKSKHKPYLGNKTR---PQKINKGILVRKFMTDMKWTVDKVFNNTNVSIRPTFQKIVLNFLIKSPFQGNWNNPLNFCKRSKQA 3021

HSP 4 Score: 73.559 bits (179), Expect = 1.388e-156
Identity = 31/43 (72.09%), Postives = 38/43 (88.37%), Query Frame = 2 
Query: 1190 IEVIFELKRADNTSPGIDGIKYYDIAIFDPEGKLLTLLYNKII 1318
             E++F+LKRADNTSPGIDGI+Y D+ + DPEGKLLT L+NKII
Sbjct: 3030 FEIMFQLKRADNTSPGIDGIQYRDLLLLDPEGKLLTFLFNKII 3072

HSP 5 Score: 66.6254 bits (161), Expect = 1.388e-156
Identity = 32/52 (61.54%), Postives = 38/52 (73.08%), Query Frame = 1 
Query: 2146 RLVNAAKKLMNSDLCGWQKLKPYKTFLHSRLTFPFRTIEIKISALSANVCNN 2301
            + V+  +KL NS+L GWQKLK YK F+HSRL FPFRT EIK  ALS +  NN
Sbjct: 3363 KAVSDTRKLANSELLGWQKLKAYKIFVHSRLIFPFRTREIKTGALSKSNGNN 3414

HSP 6 Score: 201.445 bits (511), Expect = 1.495e-156
Identity = 92/127 (72.44%), Postives = 108/127 (85.04%), Query Frame = 1 
Query: 1330 MPDVWKYFKTLMIPKPDKDDKYNLISSWRPIALLSVVYKIFASILARRLTSWVNRNNLLHIGQKWGSVHDGCVEHNSVLSSCLENSKYSKDTPTIIAFLDIKDAFDNVPHDYMWKILRYIGVDEKFT 1710
            +PD WK FKTL+ PKP+KD KY+ +SSWRPIALLSV+YK+FAS LA RLT W+N NNLLHIGQK GS HDGCVEHNS+LS+ LE+SKYSK+ P  IA+LDIKDAF +VPHDYMW +LR+IGV E FT
Sbjct: 5404 IPDSWKSFKTLLTPKPNKDGKYDDVSSWRPIALLSVIYKVFASCLASRLTYWINTNNLLHIGQKGGSRHDGCVEHNSILSAALEHSKYSKNCPLAIAWLDIKDAFGSVPHDYMWSLLRFIGVGEDFT 5530

HSP 7 Score: 181.03 bits (458), Expect = 1.495e-156
Identity = 85/128 (66.41%), Postives = 103/128 (80.47%), Query Frame = 2 
Query: 1715 LKLLYTETSSYYTCGPIVTPNIPIKQGVKQGCPLSMILFAIAINTVLQAVTLSKVKPFKIGESSIQVLAYADDIGLIANNTHDLQNILNIAFDVACEIGFEYRPEKCAYI*LPNVDTIGEISINNIQI 2098
            L+LLY++TS++Y+CGPI+TPNIPIKQGVKQGCP+SMILFA+AIN VL+AV+ S  +PF IGES +++LAYADDI LIA +  DLQ I NIA   A EIGFEYRPEKC Y+ LP V    EI INN +I
Sbjct: 5533 LQLLYSDTSTFYSCGPILTPNIPIKQGVKQGCPISMILFALAINPVLEAVSRSDCEPFMIGESPVKILAYADDIALIAKSVEDLQKITNIAVRTAAEIGFEYRPEKCGYLQLPKVHIDSEILINNTKI 5660

HSP 8 Score: 116.701 bits (291), Expect = 1.495e-156
Identity = 135/413 (32.69%), Postives = 202/413 (48.91%), Query Frame = 3 
Query:    3 AVG-NRREYNRLLE*IYS*KTIIFKKSHTLFRFVMFSDDPIHHATTFILFLEEFISGNVVNRDKVIEKLVNMQSKMCNEVVVNLNLINCKITEXXXXXXXXXXXXSKNFIFFKQYNDHSNTG*LHSMLK*IFKY*KFNE--SKNCVYHYCSSLSSANFDGHR--LQL**TLST*SFILLPNFPYLCTANS-------LLTKIKE---TPRLCKILGNCPHDIRGSGFVSKILICAFIKHNALNLSLENVNLRDIGDLIKSLLSTVQDIIKNSIKNLPDNKTNVKQ------NLDQRILLINENLLTLRNASVNEISNLIINEIRIRKETNVTKISRPYLGNKISIVPPPKTN---FKSSFNQNMKTTVYNILVNNEIKERPTIKDHISNFSHQDQVIKSYDNVLNFGLKSQNS 1169
            A+G N  EY  L + I S + +IF   H +    + SDD +  A+ FI+ +E  I    V+  +  E L +++ K  NE+ +NLN INCKI E I   +F NL L+ NF+     N+   T  L    + + K+  + +      ++   +         HR  L +   ++   +I+ P    LC   S          KI E   TP     +G+ P+DIRGSG VSK+LICAFIK+ A +L L N+NLR+IG  I SLL     II  S K L  + T + +      NL+QR L I+E L  + + +VNEI ++I+ +   R    V    +PYLGNK     P K N       F  +MK TV  +  N  +  RPT +  + NF  +     +++N LNF  +S+ +
Sbjct: 4960 AIGENMTEY--LNKFIISNRKVIFISEHNIEHASITSDDSLLFASAFIILIESLIFSKQVSHTRTSEFLKSVKIKQANEICLNLNSINCKINEEITAKYFSNLKLNSNFMLL---NETMCTAILDDCTRHLTKFLNYEDLIKAQVIFAIIAP------PKHREILLIIDYITDEYYIINPT--TLCGTESSQSVCRIFRNKINEIKETPGRVINMGDIPNDIRGSGLVSKLLICAFIKNYANDLPLINLNLREIGTNISSLLP----IISESKKILMSDLTKITKIKKSNLNLEQRKLKIDELLHDVSHLTVNEIIDIIVRQFPDR----VKSKHKPYLGNKTR---PQKINKGILVRKFMTDMKWTVDKVFNNTNVSIRPTFQKIVLNFLIKSPFHGNWNNPLNFCKRSKQA 5348

HSP 9 Score: 73.559 bits (179), Expect = 1.495e-156
Identity = 31/43 (72.09%), Postives = 38/43 (88.37%), Query Frame = 2 
Query: 1190 IEVIFELKRADNTSPGIDGIKYYDIAIFDPEGKLLTLLYNKII 1318
             E++F+LKRADNTSPGIDGI+Y D+ + DPEGKLLT L+NKII
Sbjct: 5357 FEIMFQLKRADNTSPGIDGIQYRDLLLLDPEGKLLTFLFNKII 5399

HSP 10 Score: 66.6254 bits (161), Expect = 1.495e-156
Identity = 32/52 (61.54%), Postives = 38/52 (73.08%), Query Frame = 1 
Query: 2146 RLVNAAKKLMNSDLCGWQKLKPYKTFLHSRLTFPFRTIEIKISALSANVCNN 2301
            + V+  +KL NS+L GWQKLK YK F+HSRL FPFRT EIK  ALS +  NN
Sbjct: 5690 KAVSDTRKLANSELLGWQKLKAYKIFVHSRLIFPFRTREIKTGALSKSNGNN 5741

HSP 11 Score: 176.022 bits (445), Expect = 6.293e-57
Identity = 83/128 (64.84%), Postives = 103/128 (80.47%), Query Frame = 2 
Query: 1715 LKLLYTETSSYYTCGPIVTPNIPIKQGVKQGCPLSMILFAIAINTVLQAVTLSKVKPFKIGESSIQVLAYADDIGLIANNTHDLQNILNIAFDVACEIGFEYRPEKCAYI*LPNVDTIGEISINNIQI 2098
            L+LLYT+TSS+Y C  + TP+IPIK+GVKQGCP+SMILFAIAIN VL+A++LS   PF IG SS+QVLAYADDI LIA N+ DLQ + ++A + A  IGFEYRPEKCAYI  P V+T+ EI +NN+ I
Sbjct:  916 LQLLYTDTSSFYQCNQMQTPDIPIKKGVKQGCPISMILFAIAINPVLEAISLSNTTPFMIGTSSVQVLAYADDIALIATNSSDLQTLFDLAVNTANMIGFEYRPEKCAYIQYPKVETVSEIIVNNVLI 1043

HSP 12 Score: 64.3142 bits (155), Expect = 6.293e-57
Identity = 35/64 (54.69%), Postives = 45/64 (70.31%), Query Frame = 1 
Query: 2146 RLVNAAKKLMNSDLCGWQKLKPYKTFLHSRLTFPFRTIEIKISALS-------ANVCNNTTKLR 2316
            +++   KK+ +S+L GWQKLK YK FLHSRL FPFRT EIK S+L+        NV NNT++LR
Sbjct: 1073 KVIEDTKKIAHSNLYGWQKLKAYKIFLHSRLIFPFRTREIKTSSLADPSRNSRINV-NNTSQLR 1135

HSP 13 Score: 21.9422 bits (45), Expect = 6.293e-57
Identity = 8/11 (72.73%), Postives = 11/11 (100.00%), Query Frame = 3 
Query: 2118 NQSPYEILNKV 2150
            +QSPYEI++KV
Sbjct: 1064 DQSPYEIVSKV 1074

HSP 14 Score: 122.479 bits (306), Expect = 2.114e-51
Identity = 77/185 (41.62%), Postives = 108/185 (58.38%), Query Frame = 3 
Query:  603 GNCPHDIRGSGFVSKILICAFIKHNALNLSLENVNLRDIGDLIKSLLSTV--QDIIKNSIKNLPDNKTNVKQNLDQRILLINENLLTLRNASVNEISNLIINEIRIRKETNVTKISRPYLGNKISIVPPPKTNFKSSFNQNMKTTVYNILVNNEIKERPTIKDHISNFSHQDQVIKSYDNVLNFG 1151
            G+C HDIRGSG +SKILICAFI++ AL+ SLEN+NL +IG ++ SL+  V   D +K   +        +K NL QR   IN+ L+ L ++ V+EI   I+  I   +     +   PYLG   +   P K N  + F  NMKTTVY IL +  +K  P  ++ I+NF+H D V + +DNVL F 
Sbjct:  695 GHCRHDIRGSGLLSKILICAFIRNYALDYSLENINLHEIGKIVNSLVPIVDGSDNVKYEKREKLKAANTLKLNLKQRKEKINQLLIKLSDSDVDEIICTILTNIPRLENLQKKEFREPYLGKNHN--KPKKFNNPAEFLTNMKTTVYRILNDQSVKIEPPFQEIINNFTHPDVVPECWDNVLKFS 877

HSP 15 Score: 75.485 bits (184), Expect = 2.114e-51
Identity = 32/61 (52.46%), Postives = 46/61 (75.41%), Query Frame = 1 
Query:  331 FLNNTMTTAILDDCTQCLNEFLNIENLMKAKIVFTIIAPP*AQQILMVTDYNSDEHYLLNP 513
             LN T+  AI DDCTQ + +FLNIE +MKA ++F II+PP   ++L+V DY+++EHY L+P
Sbjct:  601 LLNKTLCVAISDDCTQYMKDFLNIECMMKANVIFAIISPPKVHELLLVIDYSTEEHYYLSP 661

HSP 16 Score: 38.891 bits (89), Expect = 2.114e-51
Identity = 22/74 (29.73%), Postives = 40/74 (54.05%), Query Frame = 3 
Query:   57 KTIIFKKSHTLFRFVMFSDDPIHHATTFILFLEEFISGNVVNRDKVIEKLVNMQSKMCNEVVVNLNLINCKITE 278
            + + F  +H +      S D  + A+ FI+  E+ +    ++ DKV + L  ++++ C E+   LNLIN KIT+
Sbjct:  510 RNVKFLANHHISHSTFLSYDTQYFASHFIVLFEKLVYEIHLDCDKVYDSLSKIKTETCIEISPGLNLINGKITD 583

HSP 17 Score: 27.335 bits (59), Expect = 2.114e-51
Identity = 11/22 (50.00%), Postives = 16/22 (72.73%), Query Frame = 1 
Query:    1 KLLGIGENITDYLNKFILEKQL 66
            +L+ IG  ITDYLN+F L + +
Sbjct:  491 RLIEIGVKITDYLNQFCLNRNV 512

HSP 18 Score: 80.8777 bits (198), Expect = 3.820e-15
Identity = 35/63 (55.56%), Postives = 51/63 (80.95%), Query Frame = 1 
Query:  328 FFLNNTMTTAILDDCTQCLNEFLNIENLMKAKIVFTIIAPP*AQQILMVTDYNSDEHYLLNPS 516
              LN TM TAILDDCT+ L +FLN E+L+KA+++F IIAPP  ++IL++ DY +DE+Y++NP+
Sbjct: 2740 MLLNETMCTAILDDCTRHLTKFLNYEDLIKAQVIFAIIAPPKHREILLIIDYITDEYYIINPT 2802

HSP 19 Score: 80.8777 bits (198), Expect = 3.820e-15
Identity = 35/63 (55.56%), Postives = 51/63 (80.95%), Query Frame = 1 
Query:  328 FFLNNTMTTAILDDCTQCLNEFLNIENLMKAKIVFTIIAPP*AQQILMVTDYNSDEHYLLNPS 516
              LN TM TAILDDCT+ L +FLN E+L+KA+++F IIAPP  ++IL++ DY +DE+Y++NP+
Sbjct: 5067 MLLNETMCTAILDDCTRHLTKFLNYEDLIKAQVIFAIIAPPKHREILLIIDYITDEYYIINPT 5129
[Close]
BLAST of Reverse transcriptase vs. Planmine SMEST
Match: SMESG000071441.1 (SMESG000071441.1)

HSP 1 Score: 245.358 bits (625), Expect = 4.379e-155
Identity = 116/125 (92.80%), Postives = 119/125 (95.20%), Query Frame = 1 
Query: 1321 ENTMPDVWKYFKTLMIPKPDKDDKYNLISSWRPIALLSVVYKIFASILARRLTSWVNRNNLLHIGQKWGSVHDGCVEHNSVLSSCLENSKYSKDTPTIIAFLDIKDAFDNVPHDYMWKILRYIGV 1695
            ENTMPDVWKYFKTLMIPKPDK DKYNLISSWRPI LLSVVYKIFASILARRLTSWVNRNNLLHIGQK GSVHDGCVEHNSVL SCLE+SKYSKDTP IIAFLDIKDAF +VPHDYMWKILR+IGV
Sbjct:  148 ENTMPDVWKYFKTLMIPKPDKGDKYNLISSWRPITLLSVVYKIFASILARRLTSWVNRNNLLHIGQKGGSVHDGCVEHNSVLLSCLEHSKYSKDTPIIIAFLDIKDAFGSVPHDYMWKILRHIGV 272

HSP 2 Score: 173.711 bits (439), Expect = 4.379e-155
Identity = 84/91 (92.31%), Postives = 87/91 (95.60%), Query Frame = 2 
Query: 1793 GVKQGCPLSMILFAIAINTVLQAVTLSKVKPFKIGESSIQVLAYADDIGLIANNTHDLQNILNIAFDVACEIGFEYRPEKCAYI*LPNVDT 2065
            G ++GCPLSMILFAIAIN+VLQAVTLSKVKPFKIGESSIQVLAYADDI LIANNTHDLQNILNIAFDVA EIGFEYRPEKCAYI LPNVDT
Sbjct:  274 GCQKGCPLSMILFAIAINSVLQAVTLSKVKPFKIGESSIQVLAYADDIALIANNTHDLQNILNIAFDVAREIGFEYRPEKCAYIQLPNVDT 364

HSP 3 Score: 106.301 bits (264), Expect = 4.379e-155
Identity = 49/57 (85.96%), Postives = 53/57 (92.98%), Query Frame = 1 
Query: 2146 RLVNAAKKLMNSDLCGWQKLKPYKTFLHSRLTFPFRTIEIKISALSANVCNNTTKLR 2316
            ++V+ AKKL +SDLCGWQKLK YKTFLHSRLTFPFRT EIKISALSANVCNNTTKLR
Sbjct:  405 KVVSDAKKLADSDLCGWQKLKAYKTFLHSRLTFPFRTREIKISALSANVCNNTTKLR 461

HSP 4 Score: 82.8037 bits (203), Expect = 4.379e-155
Identity = 40/44 (90.91%), Postives = 41/44 (93.18%), Query Frame = 2 
Query: 1193 EVIFELKRADNTSPGIDGIKYYDIAIFDPEGKLLTLLYNKIITK 1324
            EVIFELKRADNTSPGIDGI Y DIAI DPEGKLLTLLYNKIIT+
Sbjct:  105 EVIFELKRADNTSPGIDGIIYNDIAILDPEGKLLTLLYNKIITE 148

HSP 5 Score: 26.5646 bits (57), Expect = 4.379e-155
Identity = 11/11 (100.00%), Postives = 11/11 (100.00%), Query Frame = 3 
Query: 2118 NQSPYEILNKV 2150
            NQSPYEILNKV
Sbjct:  396 NQSPYEILNKV 406

HSP 6 Score: 105.145 bits (261), Expect = 4.154e-39
Identity = 50/58 (86.21%), Postives = 52/58 (89.66%), Query Frame = 1 
Query:  346 MTTAILDDCTQCLNEFLNIENLMKAKIVFTIIAPP*AQQILMVTDYNSDEHYLLNPSF 519
            M TAILDDCTQ LNEFLNIENLMKAK VF IIAPP A+QILM+ DYNSDEHYLLNPSF
Sbjct:    1 MITAILDDCTQYLNEFLNIENLMKAKTVFAIIAPPKARQILMIIDYNSDEHYLLNPSF 58

HSP 7 Score: 77.0258 bits (188), Expect = 4.154e-39
Identity = 40/74 (54.05%), Postives = 50/74 (67.57%), Query Frame = 3 
Query:  516 ILLPNFPYLCTANSLLTKI---KETPRLCKILGNCPHDIRGSGFVSKILICAFIKHNALNLSLENVNLRDIGDL 728
             LLP+F YLCTANSLLTKI   KETP LCK+LGNC HDIRGSG V+K +I    + +  +  ++ +   DI  L
Sbjct:   58 FLLPHFLYLCTANSLLTKINEIKETPELCKLLGNCSHDIRGSGLVNKEVIFELKRADNTSPGIDGIIYNDIAIL 131
The following BLAST results are available for this feature:
BLAST of Reverse transcriptase vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Reverse transcriptase vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Reverse transcriptase vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Reverse transcriptase vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
si:dkeyp-88h4.28.841e-1835.97si:dkeyp-88h4.2 [Source:ZFIN;Acc:ZDB-GENE-131121-1... [more]
CU024870.21.215e-1735.97pep chromosome:GRCz11:25:23489979:23495448:1 gene:... [more]
BX544877.21.374e-1735.97pep chromosome:GRCz11:25:25310063:25315481:1 gene:... [more]
CU462878.37.576e-1631.75pep chromosome:GRCz11:17:8542203:8545189:1 gene:EN... [more]
BX927081.12.158e-1528.36pep chromosome:GRCz11:6:24296039:24301324:-1 gene:... [more]
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BLAST of Reverse transcriptase vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSXETT00000040456.12.162e-1632.52pep primary_assembly:Xenopus_tropicalis_v9.1:8:873... [more]
slc7a12.209e-1630.00solute carrier family 7 (cationic amino acid trans... [more]
ENSXETT00000032317.12.612e-1632.52pep primary_assembly:Xenopus_tropicalis_v9.1:1:130... [more]
ENSXETT00000002428.13.624e-1632.33pep primary_assembly:Xenopus_tropicalis_v9.1:3:159... [more]
ENSXETT00000046003.11.065e-1530.22pep primary_assembly:Xenopus_tropicalis_v9.1:KV460... [more]
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BLAST of Reverse transcriptase vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Reverse transcriptase vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q03278|PO21_NASVI1.315e-1430.60Retrovirus-related Pol polyprotein from type-1 ret... [more]
sp|Q03274|PO22_POPJA6.424e-1429.08Retrovirus-related Pol polyprotein from type-1 ret... [more]
sp|P11369|LORF2_MOUSE8.499e-1436.99LINE-1 retrotransposable element ORF2 protein OS=M... [more]
sp|P08548|LIN1_NYCCO5.559e-1324.84LINE-1 reverse transcriptase homolog OS=Nycticebus... [more]
sp|P16423|POLR_DROME9.724e-1335.37Retrovirus-related Pol polyprotein from type-2 ret... [more]
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BLAST of Reverse transcriptase vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A159X4Q66.542e-3561.86ORF2-like protein (Fragment) OS=Schmidtea mediterr... [more]
A0A3L8D8E55.116e-3335.88Reverse transcriptase domain-containing protein OS... [more]
A0A3L8D9A55.287e-3335.88Reverse transcriptase domain-containing protein OS... [more]
A0A3L8DAI75.861e-3334.81Reverse transcriptase domain-containing protein OS... [more]
U4USZ68.150e-3341.96Reverse transcriptase domain-containing protein (F... [more]
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BLAST of Reverse transcriptase vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSAMXT00000034560.12.919e-2125.40pep primary_assembly:Astyanax_mexicanus-2.0:APWO02... [more]
ENSAMXT00000034831.14.391e-1635.82pep primary_assembly:Astyanax_mexicanus-2.0:3:1621... [more]
ENSAMXT00000032297.14.839e-1631.86pep primary_assembly:Astyanax_mexicanus-2.0:APWO02... [more]
ENSAMXT00000056440.11.009e-1532.58pep primary_assembly:Astyanax_mexicanus-2.0:19:132... [more]
ENSAMXT00000048276.14.925e-1539.19pep primary_assembly:Astyanax_mexicanus-2.0:17:785... [more]
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BLAST of Reverse transcriptase vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Reverse transcriptase vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Reverse transcriptase vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Reverse transcriptase vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSORLT00000040357.16.034e-1633.61pep primary_assembly:ASM223467v1:14:28942701:28945... [more]
ENSORLT00000043450.12.699e-1530.95pep primary_assembly:ASM223467v1:12:7314868:731859... [more]
ENSORLT00000037544.12.835e-1530.95pep primary_assembly:ASM223467v1:22:28708275:28712... [more]
ENSORLT00000028663.13.051e-1526.32pep primary_assembly:ASM223467v1:7:26712747:267164... [more]
ENSORLT00000043337.13.505e-1534.45pep primary_assembly:ASM223467v1:9:26106542:261102... [more]
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BLAST of Reverse transcriptase vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000079542.12.012e-2466.12SMESG000079542.1[more]
SMESG000047025.10.000e+085.45SMESG000047025.1[more]
SMESG000021486.11.102e-15672.44SMESG000021486.1[more]
SMESG000021486.11.388e-15672.44SMESG000021486.1[more]
SMESG000071441.14.379e-15592.80SMESG000071441.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30029865 ID=SMED30029865|Name=Reverse transcriptase|organism=Schmidtea mediterranea sexual|type=transcript|length=2317bp
AAGCTGTTGGGAATAGGAGAGAATATAACAGATTACTTGAATAAATTTAT
TCTTGAAAAACAATTATATTTAAGAAATCTCATACTCTCTTTCGCTTTGT
AATGTTCTCTGATGACCCTATACATCACGCTACTACTTTTATACTATTTT
TAGAAGAATTTATTTCTGGAAATGTTGTCAATAGAGATAAAGTAATTGAA
AAGCTTGTAAATATGCAATCCAAGATGTGCAATGAAGTTGTAGTAAATTT
GAATTTAATAAATTGTAAGATTACAGAAAACATTTTAACAAACTTTTTCA
TCAATTTGAATTTATCCAAAAATTTCATTTTTTTTAAACAATACAATGAC
CACAGCAATACTGGATGATTGCACTCAATGCTTAAATGAATTTTTAAATA
TTGAAAATTTAATGAAAGCAAAAATTGTGTTTACCATTATTGCTCCTCCT
TAAGCTCAGCAAATTTTGATGGTCACCGACTACAACTCTGATGAACATTA
TCTACTTAATCCTTCATTTTACTTCCAAACTTCCCTTATCTTTGTACAGC
AAATTCTTTATTGACTAAAATCAAAGAAACTCCTAGACTTTGTAAAATAC
TTGGTAATTGTCCTCATGATATTCGTGGCTCTGGTTTTGTTAGTAAGATT
CTCATATGTGCATTTATCAAACACAATGCCTTAAATTTGTCTTTGGAAAA
TGTTAATCTCCGTGATATTGGAGATCTCATAAAGTCTTTATTATCGACTG
TACAAGACATTATAAAGAATTCGATAAAGAATTTACCTGATAATAAAACA
AATGTAAAGCAAAACTTAGATCAACGCATACTTTTGATAAATGAGAATTT
GTTGACTTTACGAAACGCAAGTGTAAACGAAATCAGTAACTTGATTATTA
ATGAAATACGTATTCGTAAAGAAACAAATGTAACTAAAATCAGTAGACCA
TATTTAGGAAATAAAATCAGTATAGTTCCACCACCAAAAACTAACTTCAA
ATCATCATTTAATCAAAATATGAAAACGACGGTATACAACATTTTAGTTA
ACAATGAAATAAAAGAGAGGCCAACAATTAAAGATCACATTAGTAATTTT
AGTCATCAAGATCAGGTAATTAAAAGTTATGATAATGTTTTAAACTTTGG
TCTAAAATCTCAAAACTCTTTTACTTGGACCAATTATAGATAGAGGTTAT
TTTTGAACTGAAAAGAGCTGATAATACCTCTCCTGGAATTGATGGAATTA
AATATTACGATATTGCTATTTTTGACCCAGAAGGTAAATTGTTGACTCTT
CTTTACAACAAAATCATTACGAAAATACTATGCCGGATGTTTGGAAATAT
TTTAAAACTCTAATGATTCCTAAACCTGATAAAGATGATAAATATAATCT
GATCTCTTCTTGGCGACCAATTGCTTTGTTATCCGTTGTTTATAAAATAT
TTGCGTCAATATTAGCTAGACGCTTAACATCGTGGGTAAATCGAAACAAT
CTACTTCATATCGGGCAAAAATGGGGTTCTGTACATGATGGTTGTGTAGA
ACACAATTCTGTACTTTCATCTTGTCTTGAGAACTCGAAGTATAGTAAAG
ATACACCTACCATAATTGCATTTCTTGACATCAAAGACGCTTTTGACAAT
GTACCCCATGATTACATGTGGAAGATTTTACGATACATTGGAGTTGATGA
AAAATTCACAAACTTTGAAACTTCTATACACTGAAACTAGCTCATACTAT
ACATGTGGTCCAATTGTTACTCCGAATATTCCTATAAAGCAGGGCGTCAA
ACAAGGATGCCCTCTTTCAATGATTTTGTTTGCCATTGCAATAAATACTG
TCCTTCAAGCTGTTACTCTTTCTAAAGTAAAACCTTTCAAAATTGGTGAA
TCTTCAATCCAAGTTCTTGCTTATGCTGATGATATTGGTCTTATTGCTAA
TAATACTCATGATTTGCAAAATATCTTAAACATTGCTTTTGATGTAGCTT
GTGAAATTGGTTTTGAATATCGACCTGAAAAGTGTGCATATATTTAACTG
CCAAATGTCGACACGATTGGTGAAATCTCAATTAATAATATACAAATAAA
GAAATTATTGAGTTAAGAACCAAAGTCCTTATGAAATTTTGAATAAGGTT
GGTCAATGCTGCGAAAAAGCTAATGAATTCCGATTTGTGCGGTTGGCAGA
AGTTGAAGCCTTATAAGACCTTCCTTCACTCTCGTCTAACATTCCCATTT
AGAACGATAGAAATTAAAATTAGTGCTCTTTCTGCAAACGTTTGTAATAA
TACTACAAAACTTCGTA
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protein sequence of SMED30029865-orf-1

>SMED30029865-orf-1 ID=SMED30029865-orf-1|Name=SMED30029865-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=133bp
MYPMITCGRFYDTLELMKNSQTLKLLYTETSSYYTCGPIVTPNIPIKQGV
KQGCPLSMILFAIAINTVLQAVTLSKVKPFKIGESSIQVLAYADDIGLIA
NNTHDLQNILNIAFDVACEIGFEYRPEKCAYI*
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protein sequence of SMED30029865-orf-2

>SMED30029865-orf-2 ID=SMED30029865-orf-2|Name=SMED30029865-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=135bp
MPDVWKYFKTLMIPKPDKDDKYNLISSWRPIALLSVVYKIFASILARRLT
SWVNRNNLLHIGQKWGSVHDGCVEHNSVLSSCLENSKYSKDTPTIIAFLD
IKDAFDNVPHDYMWKILRYIGVDEKFTNFETSIH*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0002089reproductive organ
Vocabulary: INTERPRO
TermDefinition
IPR000477RT_dom
Vocabulary: molecular function
TermDefinition
GO:0003964RNA-directed DNA polymerase activity
GO:0004518nuclease activity
GO:0004519endonuclease activity
GO:0016740transferase activity
GO:0016779nucleotidyltransferase activity
GO:0016787hydrolase activity
Vocabulary: biological process
TermDefinition
GO:0006278RNA-dependent DNA biosynthetic process
GO:0090305nucleic acid phosphodiester bond hydrolysis