SMED30029830
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30029830 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 1
Homology
BLAST of SMED30029830 vs. Planmine SMEST
Match: SMESG000021486.1 (SMESG000021486.1) HSP 1 Score: 178.333 bits (451), Expect = 1.367e-52 Identity = 113/313 (36.10%), Postives = 162/313 (51.76%), Query Frame = -3 Query: 31 KV*QVQSLCEISNKINNWNPDSSNVDEIKAKRLIYFAIRDKKKFISSKKQSYRIPQTCRKICTSKMSGFIFQIIDSLVLFI---QEKPDGNHVEQFTTLNLQTSTEYTAPVVQTSLCFINKSKNSIFETPIVNIDNKVTEITNNLPTDIDEEPLHILTIKNLDVKKMTTRKDAKLLKRISK*RYVLASVSTHAALSDDTDADCSFE-----------NFDFELLFTDDDIDYYFESYIRNQKFAFLKCFVVSILCSNNSKDIFPIPSNIYEARVILCPININNCHWILFVYCKASCNSYFIDPIFKNKRRFEN 927 K Q+++L +SN IN +S + KAKR ++F IRDKKKF+ K SYRIP CRKICT+ M+ IF II SL+L + + + + T +N S E S+ I S+ ET + + L DIDE I T ++S A +S++TDAD + + + D + FTDDDID Y +++I + +FA + CF+V IL +N +D+FPIP +++A++ILCP+NI+ HWILFVYCK S SYFIDPI + + RF N Sbjct: 4541 KATQLKALQNLSNNINAIKSESDDETIAKAKRFLFFVIRDKKKFLLGSKYSYRIPLHCRKICTTNMTTHIFSIIQSLLLTLTVSDSEQIADRSDSITLVNTTQSVEINPGTQSGSIVDI-----SVVETG----STEHARVKRRLEVDIDEVESIIHT-----------------------------TISKSALISEETDADSTIKIIEINKPLNESDRDPTIWFTDDDIDLYLKNHISSLEFASINCFIVEILRTNPGEDLFPIPEKVFKAQIILCPLNIDKTHWILFVYCKNSLTSYFIDPILQYRHRFVN 4815 HSP 2 Score: 47.7506 bits (112), Expect = 1.367e-52 Identity = 21/40 (52.50%), Postives = 28/40 (70.00%), Query Frame = -1 Query: 915 QWKLSGEIDIIHNIISSPTINSVNSFFDLLDKTFAEKSNR 1034 QWKLS EID +HNIIS TI +NSF +DK + +K+ + Sbjct: 4505 QWKLSSEIDRLHNIISPETIKHINSFLSEIDKVYPQKATQ 4544 HSP 3 Score: 177.563 bits (449), Expect = 1.952e-52 Identity = 114/313 (36.42%), Postives = 162/313 (51.76%), Query Frame = -3 Query: 31 KV*QVQSLCEISNKINNWNPDSSNVDEIKAKRLIYFAIRDKKKFISSKKQSYRIPQTCRKICTSKMSGFIFQIIDSLVLFI---QEKPDGNHVEQFTTLNLQTSTEYTAPVVQTSLCFINKSKNSIFETPIVNIDNKVTEITNNLPTDIDEEPLHILTIKNLDVKKMTTRKDAKLLKRISK*RYVLASVSTHAALSDDTDADCSFE-----------NFDFELLFTDDDIDYYFESYIRNQKFAFLKCFVVSILCSNNSKDIFPIPSNIYEARVILCPININNCHWILFVYCKASCNSYFIDPIFKNKRRFEN 927 K Q+++L +SN IN +S + KAKR ++F IRDKKKF+ K SYRIP CRKICT+ M+ IF II SL+L + + + + T +N S E S+ I S+ ET + + L DIDE V+ + ISK A +S +TDAD + + + D + FTDDDID Y +++I + +FA + CF+V IL +N +D+FPIP +++A++ILCP+NI+ HWILFVYCK S SYFIDPI + + RF N Sbjct: 2192 KATQLKALQNLSNNINAIKSESDDETIAKAKRFLFFVIRDKKKFLLGSKYSYRIPLHCRKICTTNMTTHIFSIIQSLLLTLTVSDSEQIADRSDSITLVNTTQSVEINPGTQSGSIVDI-----SVVETG----STEHARVKRRLEVDIDE------------VESIIHTTSGTQQNHISK----------SALISKETDADSTIKIIEINKPLNESDRDPTIWFTDDDIDLYLKNHISSLEFASINCFIVEILRTNPGEDLFPIPEKVFKAQIILCPLNIDKTHWILFVYCKNSLTSYFIDPILQYRHRFVN 2473 HSP 4 Score: 47.7506 bits (112), Expect = 1.952e-52 Identity = 21/40 (52.50%), Postives = 28/40 (70.00%), Query Frame = -1 Query: 915 QWKLSGEIDIIHNIISSPTINSVNSFFDLLDKTFAEKSNR 1034 QWKLS EID +HNIIS TI +NSF +DK + +K+ + Sbjct: 2156 QWKLSSEIDRLHNIISPETIKHINSFLSEIDKVYPQKATQ 2195 HSP 5 Score: 141.739 bits (356), Expect = 6.936e-37 Identity = 98/289 (33.91%), Postives = 155/289 (53.63%), Query Frame = -3 Query: 7 EIKAKRLIYFAIRDKKKFISSKKQSYRIPQTCRKICTSKMSGFIFQIIDSLVLFIQEKPDGNHVEQFTTLNLQTST-EYTAPVVQTSL-CFINKSKNSIFETPIVNIDNKVTEITNNLPTDIDEEPLHILTIKNLDVKKMTTRKDAKLLKRI----SK*RYVLASVSTHAALSDDT--DADCSFENFDFELLFTDDDIDYYFESYIRNQKFAFLKCFVVSILCSNNSKDIFPIPSNIYEARVILCPININNCHWILFVYCKASCNSYFIDPIFKNKRRFENNERTHIIN 849 ++K+KR IYF +RD KK++ + Q++R+ +TC K CTS+MS I II L + D + +EQ L+ + T P+V + C NSI E I+ + I N D + ++ + + + + D+ + + I +K +V + T + + +N D E+ F+D+DID+Y E++I N ++KCF+V+ILCSN +++ IP +I A ++ CP+NINN HWILFVYCK S +SYFIDPI KN+ N + IN Sbjct: 15 DVKSKRFIYFLLRDSKKYLI-RNQTFRLFETCNKYCTSEMSQQISLIITKLKPIVINVKDVDLIEQDCDLDKPIENIDITKPIVIEEIHCM---KCNSILEKCILVREGMNVNICENCLLD----AISVVEPDSNESNQTNSNHDSPITENIINPPTKRTGQRNTVKKQPSKPKSTICNKYLTIDNRDPEIWFSDNDIDWYLETHIDNPMHGYIKCFIVNILCSNIQEEVVAIPRSISNAEMVFCPLNINNSHWILFVYCKKSHSSYFIDPILKNRNNLINKQMALKIN 295
BLAST of SMED30029830 vs. Planmine SMEST
Match: SMESG000013454.1 (SMESG000013454.1) HSP 1 Score: 177.948 bits (450), Expect = 1.885e-49 Identity = 115/315 (36.51%), Postives = 161/315 (51.11%), Query Frame = -3 Query: 31 FL*FVRQNLC*KV*QVQSLCEISNKINNWNPDSSNVDEIKAKRLIYFAIRDKKKFISSKKQSYRIPQTCRKICTSKMSGFIFQIIDSLVLFIQEKPDGNHVEQFTTLNLQTSTEYTAPVVQTSLCFINKSKNSIFETPIVNIDNKVTEITNNLPTDIDEEPLHILTIKNLDVKKMTTRKDAKLLKRISK*RYVLASVSTHAALSDDTDADCSFENFDF-----ELLFTDDDIDYYFESYIRNQKFAFLKCFVVSILCSNNSKDIFPIPSNIYEARVILCPININNCHWILFVYCKASCNSYFIDPIFKNKRRFEN 960 FL + Q+ K Q++ L +S KINN D+ D +KAKR++YF IRDKK+F++ +YRIP CRKICT KMS FI ++I S N +FE +DN + N + EP+++LT + V ASVS +S++TDAD + N+ F ++ FTDDDIDYY ES+I N FA +KCF+V IL SN +IFP+P N+++A++ILCP+NIN HWI+FVYCK + SYF+DPI + + N Sbjct: 307 FLGVIDQSYTTKSDQLKFLTSLSEKINNIEIDNDVDDLVKAKRMLYFIIRDKKRFLNKDNFNYRIPFNCRKICTLKMSDFITKLIQS--------------------------------------------NELFEEREAVVDNNIDVSPNENLNILIVEPVNLLT------------------------QEVPASVSISVPISEETDADSTIINYFFPERDPKIWFTDDDIDYYLESHIGNPNFAPVKCFIVEILSSNCKDEIFPMPDNLFKAQIILCPLNINESHWIVFVYCKLTKISYFVDPILRKRHNLIN 553
BLAST of SMED30029830 vs. Planmine SMEST
Match: SMESG000021486.1 (SMESG000021486.1) HSP 1 Score: 165.236 bits (417), Expect = 9.153e-49 Identity = 106/302 (35.10%), Postives = 152/302 (50.33%), Query Frame = -3 Query: 31 KV*QVQSLCEISNKINNWNPDSSNVDEIKAKRLIYFAIRDKKKFISSKKQSYRIPQTCRKICTSKMSGFIFQIIDSLVLFI---QEKPDGNHVEQFTTLNLQTSTEYTAPVVQTSLCFINKSKNSIFETPIVNIDNKVTEITNNLPTDIDEEPLHILTIKNLDVKKMTTRKDAKLLKRISK*RYVLASVSTHAALSDDTDADCSFENFDFELLFTDDDIDYYFESYIRNQKFAFLKCFVVSILCSNNSKDIFPIPSNIYEARVILCPININNCHWILFVYCKASCNSYFIDPIFKNKRRFEN 927 K Q+++L +SN IN +S + KAKR ++F IRDKKKF+ K SYRIP CRKICT+ M+ IF II SL+L + + + + T +N S E S+ I+ + T + ++ E TD D TIK +++ K D D + FTDDDID Y +++I + +FA + CF+V IL +N +D+FPIP +++A++ILCP+NI+ HWILFVYCK S SYFIDPI + + RF N Sbjct: 2192 KATQLKALQNLSNNINAIKSESDDETIAKAKRFLFFVIRDKKKFLLGSKYSYRIPLHCRKICTTNMTTHIFSIIQSLLLTLTVSDSEQIADRSDSITLVNTTQSVEINPGTQSGSIVDISVVETG--STEHARVKRRLEE------TDADS------TIKIIEINKPLNESDR-----------------------------------DPTIWFTDDDIDLYLKNHISSLEFASINCFIVEILRTNPGEDLFPIPEKVFKAQIILCPLNIDKTHWILFVYCKNSLTSYFIDPILQYRHRFVN 2444 HSP 2 Score: 47.7506 bits (112), Expect = 9.153e-49 Identity = 21/40 (52.50%), Postives = 28/40 (70.00%), Query Frame = -1 Query: 915 QWKLSGEIDIIHNIISSPTINSVNSFFDLLDKTFAEKSNR 1034 QWKLS EID +HNIIS TI +NSF +DK + +K+ + Sbjct: 2156 QWKLSSEIDRLHNIISPETIKHINSFLSEIDKVYPQKATQ 2195 HSP 3 Score: 165.236 bits (417), Expect = 9.540e-49 Identity = 107/302 (35.43%), Postives = 148/302 (49.01%), Query Frame = -3 Query: 31 KV*QVQSLCEISNKINNWNPDSSNVDEIKAKRLIYFAIRDKKKFISSKKQSYRIPQTCRKICTSKMSGFIFQIIDSLVLFI---QEKPDGNHVEQFTTLNLQTSTEYTAPVVQTSLCFINKSKNSIFETPIVNIDNKVTEITNNLPTDIDEEPLHILTIKNLDVKKMTTRKDAKLLKRISK*RYVLASVSTHAALSDDTDADCSFENFDFELLFTDDDIDYYFESYIRNQKFAFLKCFVVSILCSNNSKDIFPIPSNIYEARVILCPININNCHWILFVYCKASCNSYFIDPIFKNKRRFEN 927 K Q+++L +SN IN +S + KAKR ++F IRDKKKF+ K SYRIP CRKICT+ M+ IF II SL+L + + + + T +N S E S+ I S+ ET + + L DIDE I T N D + FTDDDID Y +++I + +FA + CF+V IL +N +D+FPIP +++A++ILCP+NI+ HWILFVYCK S SYFIDPI + + RF N Sbjct: 4512 KATQLKALQNLSNNINAIKSESDDETIAKAKRFLFFVIRDKKKFLLGSKYSYRIPLHCRKICTTNMTTHIFSIIQSLLLTLTVSDSEQIADRSDSITLVNTTQSVEINPGTQSGSIVDI-----SVVETG----STEHARVKRRLEVDIDEVESIIHTT-----------------------------------------------NRDPTIWFTDDDIDLYLKNHISSLEFASINCFIVEILRTNPGEDLFPIPEKVFKAQIILCPLNIDKTHWILFVYCKNSLTSYFIDPILQYRHRFVN 4757 HSP 4 Score: 47.7506 bits (112), Expect = 9.540e-49 Identity = 21/40 (52.50%), Postives = 28/40 (70.00%), Query Frame = -1 Query: 915 QWKLSGEIDIIHNIISSPTINSVNSFFDLLDKTFAEKSNR 1034 QWKLS EID +HNIIS TI +NSF +DK + +K+ + Sbjct: 4476 QWKLSSEIDRLHNIISPETIKHINSFLSEIDKVYPQKATQ 4515 HSP 5 Score: 142.895 bits (359), Expect = 3.008e-37 Identity = 98/289 (33.91%), Postives = 155/289 (53.63%), Query Frame = -3 Query: 7 EIKAKRLIYFAIRDKKKFISSKKQSYRIPQTCRKICTSKMSGFIFQIIDSLVLFIQEKPDGNHVEQFTTLNLQTST-EYTAPVVQTSL-CFINKSKNSIFETPIVNIDNKVTEITNNLPTDIDEEPLHILTIKNLDVKKMTTRKDAKLLKRI----SK*RYVLASVSTHAALSDDT--DADCSFENFDFELLFTDDDIDYYFESYIRNQKFAFLKCFVVSILCSNNSKDIFPIPSNIYEARVILCPININNCHWILFVYCKASCNSYFIDPIFKNKRRFENNERTHIIN 849 ++K+KR IYF +RD KK++ + Q++R+ +TC K CTS+MS I II L + D + +EQ L+ + T P+V + C NSI E I+ + I N D + ++ + + + + D+ + + I +K +V + T + + +N D E+ F+D+DID+Y E++I N ++KCF+V+ILCSN +++ IP +I A ++ CP+NINN HWILFVYCK S +SYFIDPI KN+ N + IN Sbjct: 15 DVKSKRFIYFLLRDSKKYLI-RNQTFRLFETCNKYCTSEMSQQISLIITKLKPIVINVKDVDLIEQDCDLDKPIENIDITKPIVIEEIHCM---KCNSILEKCILVREGMNVNICENCLLD----AISVVEPDSNESNQTNSNHDSPITENIINPPTKRTGQRNTVKKQPSKPKSTICNKYLTIDNRDPEIWFSDNDIDWYLETHIDNPMHGYIKCFIVNILCSNIQEEVVAIPRSISNAEMVFCPLNINNSHWILFVYCKKSHSSYFIDPILKNRNNLINKQMALKIN 295
BLAST of SMED30029830 vs. Planmine SMEST
Match: SMESG000059599.1 (SMESG000059599.1) HSP 1 Score: 133.265 bits (334), Expect = 9.378e-36 Identity = 66/76 (86.84%), Postives = 70/76 (92.11%), Query Frame = -3 Query: 646 NPDSSNVDEIKAKRLIYFAIRDKKKFISSKKQSYRIPQTCRKICTSKMSGFIFQIIDSLVLFIQEKPDGNHVEQFT 873 NPDSS+VDEIKAK LIYFAIRDK+KFISSKKQSYRIPQTCRKICTSKMSGFIFQIIDSLVLFI+EK D N V + T Sbjct: 59 NPDSSSVDEIKAKCLIYFAIRDKEKFISSKKQSYRIPQTCRKICTSKMSGFIFQIIDSLVLFIEEKSDVNQVAKPT 134
BLAST of SMED30029830 vs. Planmine SMEST
Match: SMESG000078596.1 (SMESG000078596.1) HSP 1 Score: 126.331 bits (316), Expect = 1.040e-31 Identity = 60/116 (51.72%), Postives = 82/116 (70.69%), Query Frame = -3 Query: 52 VLASVSTHAALSDDTDADCS-----FENFDFELLFTDDDIDYYFESYIRNQKFAFLKCFVVSILCSNNSKDIFPIPSNIYEARVILCPININNCHWILFVYCKASCNSYFIDPIFK 384 + S ++S++TDAD + F D ++ FTDDDIDYY ES+I N FA +KCF+V IL SN +IFP+P N+++A++ILCP+NIN HWILFVYCK + SYF+DPIF+ Sbjct: 64 IFPKYSISVSISEETDADSTINYYFFPERDPKIWFTDDDIDYYLESHIGNPNFAPVKCFIVEILSSNCKDEIFPMPDNLFKAQIILCPLNINESHWILFVYCKLTKISYFVDPIFR 179 The following BLAST results are available for this feature:
BLAST of SMED30029830 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30029830 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30029830 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30029830 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30029830 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30029830 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30029830 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30029830 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30029830 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30029830 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30029830 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30029830 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30029830 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30029830 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30029830 ID=SMED30029830|Name=SMED30029830|organism=Schmidtea mediterranea sexual|type=transcript|length=1034bpback to top protein sequence of SMED30029830-orf-1 >SMED30029830-orf-1 ID=SMED30029830-orf-1|Name=SMED30029830-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=114bp MSGFIFQIIDSLVLFIQEKPDGNHVEQFTTLNLQTSTEYTAPVVQTSLCFback to top Annotated Terms
The following terms have been associated with this transcript:
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