IRS-type PTB domain-containing protein

Overview


Name	IRS-type PTB domain-containing protein
Smed ID	SMED30029829
Length (bp)	1308

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of IRS-type PTB domain-containing protein (SMED30029829) t-SNE clustered cells

Violin plots show distribution of expression levels for IRS-type PTB domain-containing protein (SMED30029829) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of IRS-type PTB domain-containing protein (SMED30029829) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for IRS-type PTB domain-containing protein (SMED30029829) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30029829

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 4

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
oocyte	SMED30029829	SMESG000039406.1	Contig12032	uc_Smed_v2	PMID:28434803 Rouhana et al., 2017
vitelline gland	SMED30029829	SMESG000039406.1	Contig12032	uc_Smed_v2	PMID:28434803 Rouhana et al., 2017
oocyte	SMED30029829	SMESG000039406.1	Contig12032	newmark_ests	PMID:28434803 Rouhana et al., 2017
vitelline gland	SMED30029829	SMESG000039406.1	Contig12032	newmark_ests	PMID:28434803 Rouhana et al., 2017

Note: Hover over icons to view figure legend

Homology

[Close]

BLAST of IRS-type PTB domain-containing protein vs. Ensembl Human
Match: DOK6 (docking protein 6 [Source:HGNC Symbol;Acc:HGNC:28301])

HSP 1 Score: 48.1358 bits (113), Expect = 3.641e-6
Identity = 17/43 (39.53%), Postives = 30/43 (69.77%), Query Frame = 2

Query:  566 RLRTFVWPIETVRHFGFKENFFSFESGRYSSTGEGRFTFKIKK 694
            +++  +WP+ ++R +G    +F+FESGR   TGEG FTF+ ++
Sbjct:   77 KVKLVMWPLSSLRRYGRDSTWFTFESGRMCDTGEGLFTFQTRE 119

[Close]

BLAST of IRS-type PTB domain-containing protein vs. Ensembl Human
Match: FRS3 (fibroblast growth factor receptor substrate 3 [Source:HGNC Symbol;Acc:HGNC:16970])

HSP 1 Score: 47.7506 bits (112), Expect = 5.078e-6
Identity = 21/48 (43.75%), Postives = 26/48 (54.17%), Query Frame = 2

Query:  551 VLPRDRLRTFVWPIETVRHFGFKENFFSFESGRYSSTGEGRFTFKIKK 694
            VL   R     WP   +R +G+  N FSFESGR   TG+G F FK  +
Sbjct:   70 VLHLHRREAVRWPYLCLRRYGYDSNLFSFESGRRCQTGQGIFAFKCSR 117

[Close]

BLAST of IRS-type PTB domain-containing protein vs. Ensembl Zebrafish
Match: dok6 (docking protein 6 [Source:ZFIN;Acc:ZDB-GENE-070410-50])

HSP 1 Score: 49.6766 bits (117), Expect = 3.419e-6
Identity = 20/58 (34.48%), Postives = 34/58 (58.62%), Query Frame = 2

Query:  566 RLRTFVWPIETVRHFGFKENFFSFESGRYSSTGEGRFTFKIKKLVLFLSNFQSVKESL 739
            R++  +WP+ ++R +G    +F+FESGR   TGEG FTF+ ++  +      S   S+
Sbjct:  152 RVKLVMWPLNSLRRYGRDSTWFTFESGRMCDTGEGLFTFQTREGEMIYQRVHSATLSI 209

[Close]

BLAST of IRS-type PTB domain-containing protein vs. Ensembl Zebrafish
Match: dok6 (docking protein 6 [Source:ZFIN;Acc:ZDB-GENE-070410-50])

HSP 1 Score: 49.6766 bits (117), Expect = 3.838e-6
Identity = 20/58 (34.48%), Postives = 34/58 (58.62%), Query Frame = 2

Query:  566 RLRTFVWPIETVRHFGFKENFFSFESGRYSSTGEGRFTFKIKKLVLFLSNFQSVKESL 739
            R++  +WP+ ++R +G    +F+FESGR   TGEG FTF+ ++  +      S   S+
Sbjct:  173 RVKLVMWPLNSLRRYGRDSTWFTFESGRMCDTGEGLFTFQTREGEMIYQRVHSATLSI 230

[Close]

BLAST of IRS-type PTB domain-containing protein vs. Ensembl Cavefish
Match: dok6 (docking protein 6 [Source:NCBI gene;Acc:103027524])

HSP 1 Score: 49.2914 bits (116), Expect = 3.771e-6
Identity = 18/43 (41.86%), Postives = 30/43 (69.77%), Query Frame = 2

Query:  566 RLRTFVWPIETVRHFGFKENFFSFESGRYSSTGEGRFTFKIKK 694
            R++  +WP+ ++R +G    +F+FESGR   TGEG FTF+ ++
Sbjct:  173 RVKLVMWPLSSLRRYGRDSTWFTFESGRMCDTGEGLFTFQTRE 215

[Close]

BLAST of IRS-type PTB domain-containing protein vs. Ensembl Cavefish
Match: dok6 (docking protein 6 [Source:NCBI gene;Acc:103027524])

HSP 1 Score: 49.2914 bits (116), Expect = 3.910e-6
Identity = 18/43 (41.86%), Postives = 30/43 (69.77%), Query Frame = 2

Query:  566 RLRTFVWPIETVRHFGFKENFFSFESGRYSSTGEGRFTFKIKK 694
            R++  +WP+ ++R +G    +F+FESGR   TGEG FTF+ ++
Sbjct:  173 RVKLVMWPLSSLRRYGRDSTWFTFESGRMCDTGEGLFTFQTRE 215

[Close]

BLAST of IRS-type PTB domain-containing protein vs. Ensembl Sea Lamprey
Match: dok6 (docking protein 6 [Source:ZFIN;Acc:ZDB-GENE-070410-50])

HSP 1 Score: 48.1358 bits (113), Expect = 1.322e-6
Identity = 19/40 (47.50%), Postives = 26/40 (65.00%), Query Frame = 2

Query:  566 RLRTFVWPIETVRHFGFKENFFSFESGRYSSTGEGRFTFK 685
            RLR   WP+ ++R +G     F+FE+GR   TGEG FTF+
Sbjct:   79 RLRLLTWPLVSLRRYGRDATRFTFEAGRSCDTGEGLFTFQ 118

[Close]

BLAST of IRS-type PTB domain-containing protein vs. Ensembl Sea Lamprey
Match: dok6 (docking protein 6 [Source:ZFIN;Acc:ZDB-GENE-070410-50])

HSP 1 Score: 47.7506 bits (112), Expect = 2.278e-6
Identity = 19/43 (44.19%), Postives = 28/43 (65.12%), Query Frame = 2

Query:  566 RLRTFVWPIETVRHFGFKENFFSFESGRYSSTGEGRFTFKIKK 694
            R+R   WP+ ++R +G     F+FESGR   TGEG FTF+ ++
Sbjct:  151 RVRLVSWPLSSLRRYGRDPTRFTFESGRMCDTGEGLFTFQTRE 193

[Close]

BLAST of IRS-type PTB domain-containing protein vs. Ensembl Sea Lamprey
Match: frs2a (fibroblast growth factor receptor substrate 2a [Source:ZFIN;Acc:ZDB-GENE-060503-462])

HSP 1 Score: 47.3654 bits (111), Expect = 4.503e-6
Identity = 21/45 (46.67%), Postives = 27/45 (60.00%), Query Frame = 2

Query:  560 RDRLRTFVWPIETVRHFGFKENFFSFESGRYSSTGEGRFTFKIKK 694
            RD +R   WP   +R +G+  N FSFESGR   TG+G F FK  +
Sbjct:   51 RDAVR---WPYTCLRRYGYDSNLFSFESGRRCQTGQGIFAFKCAR 92

[Close]

BLAST of IRS-type PTB domain-containing protein vs. Ensembl Medaka
Match: dok6 (docking protein 6 [Source:NCBI gene;Acc:101168752])

HSP 1 Score: 50.8322 bits (120), Expect = 1.197e-6
Identity = 30/96 (31.25%), Postives = 50/96 (52.08%), Query Frame = 2

Query:  566 RLRTFVWPIETVRHFGFKENFFSFESGRYSSTGEGRFTFKIKKLVLFLSNFQSVKESLYHANAKPKVNGKYLESSEISPEYYNLTAPDSYQMSRSA 853
            RL+  +WP+ ++R +G    +F+FESGR   TGEG FTF+ ++  +      S   +L  A    ++  +  +SS+I   +   T   S  + RSA
Sbjct:  173 RLKLVMWPLSSLRRYGRDSTWFTFESGRMCDTGEGLFTFQTREGEMIYQRVHSA--TLAIAQQHERMMEEMEKSSQI---HSRETITKSISLPRSA 263

[Close]

BLAST of IRS-type PTB domain-containing protein vs. Ensembl Medaka
Match: dok1b (docking protein 1 [Source:NCBI gene;Acc:101173260])

HSP 1 Score: 50.447 bits (119), Expect = 3.040e-6
Identity = 23/55 (41.82%), Postives = 32/55 (58.18%), Query Frame = 2

Query:  542 ENDVL----PRDRLRTFVWPIETVRHFGFKENFFSFESGRYSSTGEGRFTFKIKK 694
            E+DVL    P+ + R  VWP + +R +G     FSFE+GR   +G G FTF  K+
Sbjct:  187 ESDVLILKEPKTKRRVLVWPYKLLRRYGRDRVMFSFEAGRRCESGPGNFTFDTKQ 241

[Close]

BLAST of IRS-type PTB domain-containing protein vs. Ensembl Medaka
Match: dok1b (docking protein 1 [Source:NCBI gene;Acc:101173260])

HSP 1 Score: 50.0618 bits (118), Expect = 3.123e-6
Identity = 23/55 (41.82%), Postives = 32/55 (58.18%), Query Frame = 2

Query:  542 ENDVL----PRDRLRTFVWPIETVRHFGFKENFFSFESGRYSSTGEGRFTFKIKK 694
            E+DVL    P+ + R  VWP + +R +G     FSFE+GR   +G G FTF  K+
Sbjct:  187 ESDVLILKEPKTKRRVLVWPYKLLRRYGRDRVMFSFEAGRRCESGPGNFTFDTKQ 241

[Close]

BLAST of IRS-type PTB domain-containing protein vs. Ensembl Medaka
Match: frs2b (fibroblast growth factor receptor substrate 2 [Source:NCBI gene;Acc:101158925])

HSP 1 Score: 49.2914 bits (116), Expect = 5.117e-6
Identity = 24/59 (40.68%), Postives = 31/59 (52.54%), Query Frame = 2

Query:  560 RDRLRTFVWPIETVRHFGFKENFFSFESGRYSSTGEGRFTFKIKKLVLFLSNFQSVKES 736
            RD +R   WP   +R +G+  N FSFESGR   TG+G F FK  +     +  Q V  S
Sbjct:   52 RDDVR---WPYLCLRRYGYDSNLFSFESGRRCQTGQGIFAFKCSRAEEIFNMLQEVMHS 107

[Close]

BLAST of IRS-type PTB domain-containing protein vs. Ensembl Medaka
Match: frs2b (fibroblast growth factor receptor substrate 2 [Source:NCBI gene;Acc:101158925])

HSP 1 Score: 49.2914 bits (116), Expect = 5.929e-6
Identity = 24/59 (40.68%), Postives = 31/59 (52.54%), Query Frame = 2

Query:  560 RDRLRTFVWPIETVRHFGFKENFFSFESGRYSSTGEGRFTFKIKKLVLFLSNFQSVKES 736
            RD +R   WP   +R +G+  N FSFESGR   TG+G F FK  +     +  Q V  S
Sbjct:   52 RDDVR---WPYLCLRRYGYDSNLFSFESGRRCQTGQGIFAFKCSRAEEIFNMLQEVMHS 107

[Close]

BLAST of IRS-type PTB domain-containing protein vs. Planmine SMEST
Match: SMESG000039406.1 (SMESG000039406.1)

HSP 1 Score: 676.396 bits (1744), Expect = 0.000e+0
Identity = 341/341 (100.00%), Postives = 341/341 (100.00%), Query Frame = 2

Query:  128 MGVFWSQGIKCDKKLIKTLIDNETWFDATLIEYSASSIFNKIDWNFTLPRSSKKGNSKSSVLSTGYILNKSRKCFGRITVQDDCIIFQGINCNELLKAIVAHQDKSPTSADSNFSEALDNSVPYXXXXXXXXXXXXXIENDVLPRDRLRTFVWPIETVRHFGFKENFFSFESGRYSSTGEGRFTFKIKKLVLFLSNFQSVKESLYHANAKPKVNGKYLESSEISPEYYNLTAPDSYQMSRSALNGGLDRTKSNSQSTEYFFNSYHSASSPATERSYRNLPSSINYCKIDFSESYLNAQANPNGRRRVKSNGTLSKTGESIHYLTICENQTKAVEKVLQRIG 1150
            MGVFWSQGIKCDKKLIKTLIDNETWFDATLIEYSASSIFNKIDWNFTLPRSSKKGNSKSSVLSTGYILNKSRKCFGRITVQDDCIIFQGINCNELLKAIVAHQDKSPTSADSNFSEALDNSVPYNNSASSASTTSGGIENDVLPRDRLRTFVWPIETVRHFGFKENFFSFESGRYSSTGEGRFTFKIKKLVLFLSNFQSVKESLYHANAKPKVNGKYLESSEISPEYYNLTAPDSYQMSRSALNGGLDRTKSNSQSTEYFFNSYHSASSPATERSYRNLPSSINYCKIDFSESYLNAQANPNGRRRVKSNGTLSKTGESIHYLTICENQTKAVEKVLQRIG
Sbjct:    1 MGVFWSQGIKCDKKLIKTLIDNETWFDATLIEYSASSIFNKIDWNFTLPRSSKKGNSKSSVLSTGYILNKSRKCFGRITVQDDCIIFQGINCNELLKAIVAHQDKSPTSADSNFSEALDNSVPYNNSASSASTTSGGIENDVLPRDRLRTFVWPIETVRHFGFKENFFSFESGRYSSTGEGRFTFKIKKLVLFLSNFQSVKESLYHANAKPKVNGKYLESSEISPEYYNLTAPDSYQMSRSALNGGLDRTKSNSQSTEYFFNSYHSASSPATERSYRNLPSSINYCKIDFSESYLNAQANPNGRRRVKSNGTLSKTGESIHYLTICENQTKAVEKVLQRIG 341

[Close]

BLAST of IRS-type PTB domain-containing protein vs. Planmine SMEST
Match: SMESG000059045.1 (SMESG000059045.1)

HSP 1 Score: 48.9062 bits (115), Expect = 3.580e-6
Identity = 33/101 (32.67%), Postives = 49/101 (48.51%), Query Frame = 2

Query:  578 FVWPIETVRHFGFKENFFSFESGRYSSTGEGRFTFKIKKLVLFLSNFQ-SVKESLYHANAKPKVNGKYL--------ESSEISPEYYNLT-APDSYQMSRS 850
            F WP+ T+R + +  N F F+ GR S TGEG F F   +  +    F+  + E+  H+N     +G +L        ++S I P    LT  P S Q +RS
Sbjct:  101 FSWPLTTLRWYAYTHNMFIFQCGRKSPTGEGTFIFSCNEYQILKETFEYLMNENQIHSNWD---DGTHLFPPSNFRRQNSAIEPRTEMLTLLPSSLQFNRS 198

The following BLAST results are available for this feature:

BLAST of IRS-type PTB domain-containing protein vs. Ensembl Human
BLAST of IRS-type PTB domain-containing protein vs. Ensembl Celegans
BLAST of IRS-type PTB domain-containing protein vs. Ensembl Fly
BLAST of IRS-type PTB domain-containing protein vs. Ensembl Zebrafish
BLAST of IRS-type PTB domain-containing protein vs. Ensembl Xenopus
BLAST of IRS-type PTB domain-containing protein vs. Ensembl Mouse
BLAST of IRS-type PTB domain-containing protein vs. UniProt/SwissProt
BLAST of IRS-type PTB domain-containing protein vs. TrEMBL
BLAST of IRS-type PTB domain-containing protein vs. Ensembl Cavefish
BLAST of IRS-type PTB domain-containing protein vs. Ensembl Sea Lamprey
BLAST of IRS-type PTB domain-containing protein vs. Ensembl Yeast
BLAST of IRS-type PTB domain-containing protein vs. Ensembl Nematostella
BLAST of IRS-type PTB domain-containing protein vs. Ensembl Medaka
BLAST of IRS-type PTB domain-containing protein vs. Planmine SMEST

BLAST of IRS-type PTB domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 2


Match Name	E-value	Identity	Description
DOK6	3.641e-6	39.53	docking protein 6 [Source:HGNC Symbol;Acc:HGNC:283...	[more]
FRS3	5.078e-6	43.75	fibroblast growth factor receptor substrate 3 [Sou...	[more]

BLAST of IRS-type PTB domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of IRS-type PTB domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of IRS-type PTB domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 2


Match Name	E-value	Identity	Description
dok6	3.419e-6	34.48	docking protein 6 [Source:ZFIN;Acc:ZDB-GENE-070410...	[more]
dok6	3.838e-6	34.48	docking protein 6 [Source:ZFIN;Acc:ZDB-GENE-070410...	[more]

BLAST of IRS-type PTB domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of IRS-type PTB domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of IRS-type PTB domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of IRS-type PTB domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of IRS-type PTB domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2


Match Name	E-value	Identity	Description
dok6	3.771e-6	41.86	docking protein 6 [Source:NCBI gene;Acc:103027524]	[more]
dok6	3.910e-6	41.86	docking protein 6 [Source:NCBI gene;Acc:103027524]	[more]

BLAST of IRS-type PTB domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 3


Match Name	E-value	Identity	Description
dok6	1.322e-6	47.50	docking protein 6 [Source:ZFIN;Acc:ZDB-GENE-070410...	[more]
dok6	2.278e-6	44.19	docking protein 6 [Source:ZFIN;Acc:ZDB-GENE-070410...	[more]
frs2a	4.503e-6	46.67	fibroblast growth factor receptor substrate 2a [So...	[more]

BLAST of IRS-type PTB domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of IRS-type PTB domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of IRS-type PTB domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5


Match Name	E-value	Identity	Description
dok6	1.197e-6	31.25	docking protein 6 [Source:NCBI gene;Acc:101168752]	[more]
dok1b	3.040e-6	41.82	docking protein 1 [Source:NCBI gene;Acc:101173260]	[more]
dok1b	3.123e-6	41.82	docking protein 1 [Source:NCBI gene;Acc:101173260]	[more]
frs2b	5.117e-6	40.68	fibroblast growth factor receptor substrate 2 [Sou...	[more]
frs2b	5.929e-6	40.68	fibroblast growth factor receptor substrate 2 [Sou...	[more]

BLAST of IRS-type PTB domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2


Match Name	E-value	Identity	Description
SMESG000039406.1	0.000e+0	100.00	SMESG000039406.1	[more]
SMESG000059045.1	3.580e-6	32.67	SMESG000059045.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30029829 ID=SMED30029829|Name=IRS-type PTB domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1308bp
ATAAATCATAAGTAGTTTCAATTTCAATTTATTCAATGTCTGACAGATAA
TATCTAAAAAAATTATAATTTAATTTATGAACTGAGTTAATTATTCCAAT
TTGTAAGTGAATTTTTTTTATTTTGAAATGGGTGTTTTTTGGTCGCAAGG
TATAAAATGTGATAAGAAGCTGATAAAAACATTAATTGACAATGAAACTT
GGTTCGATGCAACATTAATAGAATACAGCGCTTCTTCCATATTTAATAAA
ATCGATTGGAATTTTACTTTACCTAGGTCTTCGAAGAAAGGAAATAGCAA
ATCGAGTGTTTTAAGCACCGGTTATATTTTAAATAAATCTCGCAAATGTT
TCGGTCGAATTACTGTTCAAGACGATTGCATCATATTCCAGGGAATCAAT
TGCAACGAGCTATTGAAAGCCATTGTAGCCCATCAAGATAAATCCCCCAC
ATCTGCCGATTCCAATTTTTCTGAAGCACTTGACAACAGCGTTCCTTACA
ATAATTCTGCGTCGTCAGCTTCAACAACCAGTGGTGGAATTGAAAACGAC
GTATTGCCCAGGGATCGGCTGCGTACATTTGTCTGGCCCATTGAAACTGT
CAGGCATTTTGGATTCAAAGAAAATTTTTTTTCTTTTGAATCAGGCAGAT
ACTCATCAACTGGTGAAGGACGGTTCACATTTAAAATCAAAAAATTAGTT
TTATTTTTATCAAATTTCCAAAGTGTGAAGGAAAGTCTCTATCATGCTAA
TGCAAAACCAAAAGTCAATGGTAAATACCTGGAATCCAGTGAAATATCTC
CTGAATATTACAATCTCACAGCTCCTGATTCATATCAAATGTCCAGAAGT
GCCTTGAATGGTGGCCTAGACAGAACGAAATCTAATTCACAATCAACCGA
ATATTTCTTTAATTCCTACCATTCGGCGTCGTCGCCAGCCACTGAGAGAA
GTTATCGCAATTTGCCCTCCTCCATCAACTACTGTAAGATCGATTTCAGC
GAATCATATTTGAATGCACAAGCCAATCCCAACGGCCGTCGACGAGTGAA
ATCAAATGGGACGCTCTCAAAAACCGGAGAATCGATTCATTATTTGACTA
TTTGTGAAAATCAAACAAAAGCGGTTGAAAAAGTGTTACAAAGGATTGGT
TAAAATCGTTTTATGTGACTCTGTCTTTCCATTTGTGATTGATTGTGAGC
ATACATATGTATATATCATATGTTTATTTATTTATTTATATGTTCGTGTG
TGTGTGTGTGTTATTTGGCCCGTGATATTTTATTCGCTATGCACAATATC
AATATTTA

protein sequence of SMED30029829-orf-1

>SMED30029829-orf-1 ID=SMED30029829-orf-1|Name=SMED30029829-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=342bp
MGVFWSQGIKCDKKLIKTLIDNETWFDATLIEYSASSIFNKIDWNFTLPR
SSKKGNSKSSVLSTGYILNKSRKCFGRITVQDDCIIFQGINCNELLKAIV
AHQDKSPTSADSNFSEALDNSVPYNNSASSASTTSGGIENDVLPRDRLRT
FVWPIETVRHFGFKENFFSFESGRYSSTGEGRFTFKIKKLVLFLSNFQSV
KESLYHANAKPKVNGKYLESSEISPEYYNLTAPDSYQMSRSALNGGLDRT
KSNSQSTEYFFNSYHSASSPATERSYRNLPSSINYCKIDFSESYLNAQAN
PNGRRRVKSNGTLSKTGESIHYLTICENQTKAVEKVLQRIG*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000074	oocyte
PLANA:0000231	vitelline gland

Vocabulary: **INTERPRO**
Term	Definition
IPR002404	IRS_PTB
IPR011993	PH-like_dom_sf

Vocabulary: **molecular function**
Term	Definition
GO:0005158	insulin receptor binding

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SMART	SM01244	IRS_2	coord: 124..202 e-value: 0.0023 score: 15.4
None	No IPR available	GENE3D	G3DSA:2.30.29.250		coord: 91..237 e-value: 3.2E-13 score: 51.5
None	No IPR available	SUPERFAMILY	SSF50729	PH domain-like	coord: 150..205
IPR002404	IRS-type PTB domain	PFAM	PF02174	IRS	coord: 146..189 e-value: 1.0E-10 score: 41.3

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IRS-type PTB domain-containing protein

Zeng et. al., 2018

▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 4

Owlery

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