Neuronal acetylcholine receptor subunit alpha-4

Overview


Name	Neuronal acetylcholine receptor subunit alpha-4
Smed ID	SMED30029822
Length (bp)	2260

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of Neuronal acetylcholine receptor subunit alpha-4 (SMED30029822) t-SNE clustered cells

Violin plots show distribution of expression levels for Neuronal acetylcholine receptor subunit alpha-4 (SMED30029822) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Neuronal acetylcholine receptor subunit alpha-4 (SMED30029822) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30029822

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 12

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
head region	SMED30029822	SMESG000021568.1	SmedASXL_011119	SmedAsxl_ww_GCZZ01	PMID:27034770 Currie et al., 2016
Smed sexual biotype	SMED30029822	SMESG000021568.1	Contig36407	uc_Smed_v2	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30029822	SMESG000021568.1	Contig36407	newmark_ests	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30029822	SMESG000080004.1 SMESG000079430.1 SMESG000076652.1 SMESG000076091.1 SMESG000074857.1 SMESG000069936.1 SMESG000065288.1 SMESG000061056.1 SMESG000058180.1 SMESG000053372.1 SMESG000050587.1 SMESG000050353.1 SMESG000046659.1 SMESG000043782.1 SMESG000042816.1 SMESG000036922.1 SMESG000034701.1 SMESG000033592.1 SMESG000031049.1 SMESG000029226.1 SMESG000023611.1 SMESG000022485.1 SMESG000022236.1 SMESG000021568.1 SMESG000017989.1 SMESG000013698.1 SMESG000009566.1 SMESG000006220.1 SMESG000005221.1 SMESG000001234.1	Contig45720	uc_Smed_v2	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30029822	SMESG000080004.1 SMESG000079430.1 SMESG000076652.1 SMESG000076091.1 SMESG000074857.1 SMESG000069936.1 SMESG000065288.1 SMESG000061056.1 SMESG000058180.1 SMESG000053372.1 SMESG000050587.1 SMESG000050353.1 SMESG000046659.1 SMESG000043782.1 SMESG000042816.1 SMESG000036922.1 SMESG000034701.1 SMESG000033592.1 SMESG000031049.1 SMESG000029226.1 SMESG000023611.1 SMESG000022485.1 SMESG000022236.1 SMESG000021568.1 SMESG000017989.1 SMESG000013698.1 SMESG000009566.1 SMESG000006220.1 SMESG000005221.1 SMESG000001234.1	Contig45720	newmark_ests	PMID:29674431 Fincher et al., 2018
nervous system	SMED30029822	SMESG000021568.1	dd_Smed_v4_9503_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
cephalic ganglia	SMED30029822	SMESG000021568.1	dd_Smed_v4_9503_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
neuron	SMED30029822	SMESG000021568.1	dd_Smed_v4_9503_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
anterior side	SMED30029822	SMESG000021568.1	dd_Smed_v4_9503_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
muscle cell	SMED30029822	SMESG000021568.1	dd_Smed_v4_9503_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
cilated neuron	SMED30029822	SMESG000021568.1	dd_Smed_v4_9503_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
head region	SMED30029822	SMESG000021568.1	dd_Smed_v6_9503_0_1	dd_Smed_v6	PMID:28171748 Stückemann et al., 2017

Note: Hover over icons to view figure legend

Homology

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BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Human
Match: CHRNA4 (cholinergic receptor nicotinic alpha 4 subunit [Source:HGNC Symbol;Acc:HGNC:1958])

HSP 1 Score: 198.364 bits (503), Expect = 6.337e-54
Identity = 121/358 (33.80%), Postives = 198/358 (55.31%), Query Frame = 3

Query:  288 VEKNLTVSILNNYQRYGIFGRPVSHSKELMKVQYGLQLIQLLDLDENKQILRTNCWSMYRWHDKLLEWNPAEHGGITEIRVFPDQIWTPDIKLYNFXXXXXXXXXXXXXXXKHTGKTLWVPQSIFKSTCAVEITYFPFDTQICMLEFGSWAYDIHQLDIIWWVPDGTNVSMPYIDFNDYLQSNEWRTDGEVDFQLKLEGDNKTQIKSVKKYRTRVYVSSNGQPINKHYPXXXXXXXXXXNSSFYVLILVIPCILLSSLTLVVFWLPPESPAKMMLGMNIXXXXXXXXXXXXESTPSAVKKFPLIGAYFCLNMIMITLSTFLAALVINYYFRGDFAGPLPILVRRIIVEGLGRLLLVRQ 1361
             E+ L   + + Y ++    RPV++  +++ V++GL + QL+D+DE  Q++ TN W    WHD  L W+PA++  +T IR+  + IW PDI LYN AD       + +  + H G+  W P +I+KS+C++++T+FPFD Q C ++FGSW YD  ++D++       N+    +D  D+ +S EW                   + +V  Y TR Y     +   + YP + Y   +RR   FY + L+IPC+L+S LT++VF+LP E   K+ L +++ ++  V LLL+ E  PS     PLIG Y    MI +TLS  +   V+N + R      +P  VRR+ ++ + RLLL+++
Sbjct:   36 AEERLLKKLFSGYNKWS---RPVANISDVVLVRFGLSIAQLIDVDEKNQMMTTNVWVKQEWHDYKLRWDPADYENVTSIRIPSELIWRPDIVLYNNADGDFAVTHLTKAHLFHDGRVQWTPPAIYKSSCSIDVTFFPFDQQNCTMKFGSWTYDKAKIDLV-------NMHS-RVDQLDFWESGEW-----------------VIVDAVGTYNTRKY-----ECCAEIYPDITYAFVIRRLPLFYTINLIIPCLLISCLTVLVFYLPSECGEKITLCISVLLSLTVFLLLITEIIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHHRSPRTHTMPTWVRRVFLDIVPRLLLMKR 360

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BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Human
Match: CHRNA5 (cholinergic receptor nicotinic alpha 5 subunit [Source:HGNC Symbol;Acc:HGNC:1959])

HSP 1 Score: 194.512 bits (493), Expect = 7.330e-54
Identity = 129/382 (33.77%), Postives = 192/382 (50.26%), Query Frame = 3

Query:  291 EKNLTVSILNNYQRYGIFGRPVSHSKELMKVQYGLQLIQLLDLDENKQILRTNCWSMYRWHDKLLEWNPAEHGGITEIRVFPDQIWTPDIKLYNFXXXXXXXXXXXXXXXKHTGKTLWVPQSIFKSTCAVEITYFPFDTQICMLEFGSWAYDIHQLDIIWWVPDGTNVSMPYIDFNDYLQSNEWRTDGEVDFQLKLEGDNKTQIKSVKKYRTRVYVSSNGQPINKH-----YPXXXXXXXXXXNSSFYVLILVIPCILLSSLTLVVFWLPPESPAKMMLGMNIXXXXXXXXXXXXESTPSAVKKFPLIGAYFCLNMIMITLSTFLAALVINYYFRGDFA-GPLPILVRRIIVEGLGRLLLVRQHIPLPDETNLMDRVLTRAE 1418
            E +L   +  +Y+R+    RPV H  + +K+++GL + QL+D+DE  Q++ TN W    W D  L WNP ++GGI  IRV  D +WTPDI L++       E    + V+++ G   W P + +KS+C +++T+FPFD Q C ++FGSW YD  Q+DII    D        +D  D+  + EW                               VS+ G   N+      YP + Y   ++R   FY L L+IPCI LS LT++VF+LP     K+ L  ++ V+  V LL++ E  PS+ K  PLIG Y    MI +TLS  +    IN + R       +  LVR+I +  L +LL +R H+         DR  T+ E
Sbjct:   47 EDSLLKDLFQDYERWV---RPVEHLNDKIKIKFGLAISQLVDVDEKNQLMTTNVWLKQEWIDVKLRWNPDDYGGIKVIRVPSDSVWTPDIVLFD-NADGRFEGTSTKTVIRYNGTVTWTPPANYKSSCTIDVTFFPFDLQNCSMKFGSWTYDGSQVDIILEDQD--------VDKRDFFDNGEWEI-----------------------------VSATGSKGNRTDSCCWYPYVTYSFVIKRLPLFYTLFLIIPCIGLSFLTVLVFYLPSNEGEKICLCTSVLVSLTVFLLVIEEIIPSSSKVIPLIGEYLVFTMIFVTLSIMVTVFAINIHHRSSSTHNAMAPLVRKIFLHTLPKLLCMRSHV---------DRYFTQKE 378

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BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Human
Match: CHRNB3 (cholinergic receptor nicotinic beta 3 subunit [Source:HGNC Symbol;Acc:HGNC:1963])

HSP 1 Score: 191.43 bits (485), Expect = 8.124e-53
Identity = 126/382 (32.98%), Postives = 206/382 (53.93%), Query Frame = 3

Query:  225 MIFLIFGLFLCRGTDTVNWPSVEKNLTVSILNNYQRYGIFGRPVSHSKELMKVQYGLQLIQLLDLDENKQILRTNCWSMYRWHDKLLEWNPAEHGGITEIRVFPDQIWTPDIKLYNFXXXXXXXXXXXXXXXKHTGKTLWVPQSIFKSTCAVEITYFPFDTQICMLEFGSWAYDIHQLDIIWWVPDGTNVSMPYIDFNDYLQSNEWRTDGEVDFQLKLEGDNKTQIKSVKKYRTRVYVSSNGQPINKHYPXXXXXXXXXXNSSFYVLILVIPCILLSSLTLVVFWLPPESPAKMMLGMNIXXXXXXXXXXXXESTPSAVKKFPLIGAYFCLNMIMITLSTFLAALVINYYFRGDFA-GPLPILVRRIIVEGLGRLLLVRQHI 1367
            ++ ++ G+     + T  + S+ +N    + + +Q Y  + RPV HS + +KV +GL++ QL+D+DE  Q++ TN W    W D  L WNP ++GGI  I+V  + +W PDI L+  AD R   + + +V+VK  G  +W P + +KS+C +++T+FPFD Q C ++FGSW YD   +D+I        +    +D  D+  + EW    E+     ++G+ +  + S                    YP + Y   LRR   FY L L+IPC+ LS LT++VF+LP +   K+ L  ++ V+  V LL++ E  PS+ K  PLIG Y    MI +TLS  +   VIN + R      P+   V+R+ ++ L +LL ++ H+
Sbjct:    7 LVLIVLGI---PSSATTGFNSIAENEDALLRHLFQGYQKWVRPVLHSNDTIKVYFGLKISQLVDVDEKNQLMTTNVWLKQEWTDHKLRWNPDDYGGIHSIKVPSESLWLPDIVLFENADGRFEGSLMTKVIVKSNGTVVWTPPASYKSSCTMDVTFFPFDRQNCSMKFGSWTYDGTMVDLI--------LINENVDRKDFFDNGEW----EILNAKGMKGNRRDGVYS--------------------YPFITYSFVLRRLPLFYTLFLIIPCLGLSFLTVLVFYLPSDEGEKLSLSTSVLVSLTVFLLVIEEIIPSSSKVIPLIGEYLLFIMIFVTLSIIVTVFVINVHHRSSSTYHPMAPWVKRLFLQKLPKLLCMKDHV 353

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BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Human
Match: CHRNA2 (cholinergic receptor nicotinic alpha 2 subunit [Source:HGNC Symbol;Acc:HGNC:1956])

HSP 1 Score: 189.119 bits (479), Expect = 1.771e-51
Identity = 144/527 (27.32%), Postives = 236/527 (44.78%), Query Frame = 3

Query:  324 YQRYGIFGRPVSHSKELMKVQYGLQLIQLLDLDENKQILRTNCWSMYRWHDKLLEWNPAEHGGITEIRVFPDQIWTPDIKLYNFXXXXXXXXXXXXXXXKHTGKTLWVPQSIFKSTCAVEITYFPFDTQICMLEFGSWAYDIHQLDIIWWVPDGTNVSMPYIDFNDYLQSNEWRTDGEVDFQLKLEGDNKTQIKSVKKYRTRVYVSSNGQPINKHYPXXXXXXXXXXNSSFYVLILVIPCILLSSLTLVVFWLPPESPAKMMLGMNIXXXXXXXXXXXXESTPSAVKKFPLIGAYFCLNMIMITLSTFLAALVINYYFRGDFAGPLPILVRRIIVEGLGRLLLVRQHIPLPDETNLMDRVLTRAENGCIRKCRLKIPNLDSNKFLTSPVQTAEPPLQQDLSP------RNTNPFINNDELAESNVWQTNVRPLRSYSSVYRNTPQASMTARPNPITDTEDSPLLMRCNTTLENDVRELKRYIRMFVNRQKENNRKNLISMEWRTLALVLDRLFFVLYISSILIAVIA 1886
            ++ Y  + RPV ++ +++ V++GL + QL+D+DE  Q++ TN W    W D  L WNP + G IT +RV  + IW PDI LYN AD       + +  +  TG   WVP +I+KS+C++++T+FPFD Q C ++FGSW YD  ++D+              +D  DY +S EW               N T   + KKY     +          YP + Y   +RR   FY + L+IPC+L+S LT++VF+LP +   K+ L +++ ++  V LLL+ E  PS     PLIG Y    MI +TLS  +   V+N + R      +P  VR  ++  + R LL+ +  P                                      PV+    PL+  LSP       N +       + E + W        S  ++  +    S  + P      +   LL      L   +++    +    +  +  +  + +  +W+ +A+V+DR+F  L+I    +  I 
Sbjct:   45 FRGYNRWARPVPNTSDVVIVRFGLSIAQLIDVDEKNQMMTTNVWLKQEWSDYKLRWNPTDFGNITSLRVPSEMIWIPDIVLYNNADGEFAVTHMTKAHLFSTGTVHWVPPAIYKSSCSIDVTFFPFDQQNCKMKFGSWTYDKAKIDL--------EQMEQTVDLKDYWESGEWAI------------VNATGTYNSKKYDCCAEI----------YPDVTYAFVIRRLPLFYTINLIIPCLLISCLTVLVFYLPSDCGEKITLCISVLLSLTVFLLLITEIIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHHRSPSTHTMPHWVRGALLGCVPRWLLMNRPPP--------------------------------------PVELCH-PLRLKLSPSYHWLESNVDAEEREVVVEEEDRWACAGHVAPSVGTLCSHGHLHSGASGP------KAEALLQEGELLLSPHMQKALEGVHYIADHLRSEDADSSVKEDWKYVAMVIDRIFLWLFIIVCFLGTIG 496

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BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Human
Match: CHRNA2 (cholinergic receptor nicotinic alpha 2 subunit [Source:HGNC Symbol;Acc:HGNC:1956])

HSP 1 Score: 188.348 bits (477), Expect = 4.261e-51
Identity = 144/527 (27.32%), Postives = 236/527 (44.78%), Query Frame = 3

Query:  324 YQRYGIFGRPVSHSKELMKVQYGLQLIQLLDLDENKQILRTNCWSMYRWHDKLLEWNPAEHGGITEIRVFPDQIWTPDIKLYNFXXXXXXXXXXXXXXXKHTGKTLWVPQSIFKSTCAVEITYFPFDTQICMLEFGSWAYDIHQLDIIWWVPDGTNVSMPYIDFNDYLQSNEWRTDGEVDFQLKLEGDNKTQIKSVKKYRTRVYVSSNGQPINKHYPXXXXXXXXXXNSSFYVLILVIPCILLSSLTLVVFWLPPESPAKMMLGMNIXXXXXXXXXXXXESTPSAVKKFPLIGAYFCLNMIMITLSTFLAALVINYYFRGDFAGPLPILVRRIIVEGLGRLLLVRQHIPLPDETNLMDRVLTRAENGCIRKCRLKIPNLDSNKFLTSPVQTAEPPLQQDLSP------RNTNPFINNDELAESNVWQTNVRPLRSYSSVYRNTPQASMTARPNPITDTEDSPLLMRCNTTLENDVRELKRYIRMFVNRQKENNRKNLISMEWRTLALVLDRLFFVLYISSILIAVIA 1886
            ++ Y  + RPV ++ +++ V++GL + QL+D+DE  Q++ TN W    W D  L WNP + G IT +RV  + IW PDI LYN AD       + +  +  TG   WVP +I+KS+C++++T+FPFD Q C ++FGSW YD  ++D+              +D  DY +S EW               N T   + KKY     +          YP + Y   +RR   FY + L+IPC+L+S LT++VF+LP +   K+ L +++ ++  V LLL+ E  PS     PLIG Y    MI +TLS  +   V+N + R      +P  VR  ++  + R LL+ +  P                                      PV+    PL+  LSP       N +       + E + W        S  ++  +    S  + P      +   LL      L   +++    +    +  +  +  + +  +W+ +A+V+DR+F  L+I    +  I 
Sbjct:   67 FRGYNRWARPVPNTSDVVIVRFGLSIAQLIDVDEKNQMMTTNVWLKQEWSDYKLRWNPTDFGNITSLRVPSEMIWIPDIVLYNNADGEFAVTHMTKAHLFSTGTVHWVPPAIYKSSCSIDVTFFPFDQQNCKMKFGSWTYDKAKIDL--------EQMEQTVDLKDYWESGEWAI------------VNATGTYNSKKYDCCAEI----------YPDVTYAFVIRRLPLFYTINLIIPCLLISCLTVLVFYLPSDCGEKITLCISVLLSLTVFLLLITEIIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHHRSPSTHTMPHWVRGALLGCVPRWLLMNRPPP--------------------------------------PVELCH-PLRLKLSPSYHWLESNVDAEEREVVVEEEDRWACAGHVAPSVGTLCSHGHLHSGASGP------KAEALLQEGELLLSPHMQKALEGVHYIADHLRSEDADSSVKEDWKYVAMVIDRIFLWLFIIVCFLGTIG 518

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BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Celegans
Match: acr-11 (AcetylCholine Receptor [Source:UniProtKB/TrEMBL;Acc:P91197])

HSP 1 Score: 180.259 bits (456), Expect = 3.010e-49
Identity = 118/373 (31.64%), Postives = 192/373 (51.47%), Query Frame = 3

Query:  252 LCRGTDTVNWPSVEKNLTVSILNNYQRYGIFGRPVSHSKELMKVQYGLQLIQLLDLDENKQILRTNCWSMYRWHDKLLEWNPAEHGGITEIRVFP---DQIWTPDIKLYNFXXXXXXXXXXXXXXXKHTGKTLWVPQSIFKSTCAVEITYFPFDTQICMLEFGSWAYDIHQLDIIWWVPDGTNVSMPYIDFNDYLQSNEWRTDGEVDFQLKLEGDNKTQIKSVKKYRTRVYVSSNGQPINKHYPXXXXXXXXXXNSSFYVLILVIPCILLSSLTLVVFWLPPESPAKMMLGMNIXXXXXXXXXXXXESTPSAVKKFPLIGAYFCLNMIMITLSTFLAALVINYYFRGDFAGPLPILVRRIIVEGLGRLLLVRQ 1361
            LC G+        E  L   +L  Y       RPV +S + ++V+  L L Q+LD+DE  QI+  N W  Y W D  L+W P ++GGI +IR FP   D IW PD+ LYN A         + ++  HTG  +W+P  + K  C +++T+FPFD Q+C ++FGSW +  + +D+   + D TN +   +D + YL + EW                  Q+ S    R   Y     +P    YP + Y + +RR + +Y   L+IP +L+S + ++ F  PP++  K+ L + I +A    L +++E TP   +  PLIG +F   M++++ S     +V+N +FR   +  +  LVRR+++E L  LL + +
Sbjct:   19 LCDGS------VAETKLFTDLLKGYNP---LERPVQNSSQPLEVKIKLFLQQILDVDEKNQIVSVNAWLSYTWFDHKLQWEPKKYGGIQDIR-FPGSSDHIWKPDVLLYNSAAEDFDSTFKSNLLTYHTGTVVWIPPGVLKFVCQLDVTWFPFDDQVCEMKFGSWTFHGYAIDL--QIDDDTNGTQS-MDLSTYLVNGEW------------------QVISTNAKRVVSYYKCCPEP----YPTVNYYLHIRRRTLYYGFNLIIPSLLISLMAILGFMFPPDAGEKITLEVTILLAIVFFLSMVSEMTPPTSEAVPLIGVFFSCCMLVVSASVVFTIVVLNLHFRSADSHEMNPLVRRVLLEFLPWLLFMSR 356

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BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Celegans
Match: unc-29 (Acetylcholine receptor subunit beta-type unc-29 [Source:UniProtKB/Swiss-Prot;Acc:P48181])

HSP 1 Score: 177.948 bits (450), Expect = 3.759e-48
Identity = 149/546 (27.29%), Postives = 259/546 (47.44%), Query Frame = 3

Query:  222 WMIFLIFGLFLCRGTDTVNWPSVEKNLTVSILNNYQRYGIFGRPVSHSKEL-MKVQYGLQLIQLLDLDENKQILRTNCWSMYRWHDKLLEWNPAEHGGITEIRVFPDQIWTPDIKLYNFXXXXXXXXXXXXXXXKHTGKTLWVPQSIFKSTCAVEITYFPFDTQICMLEFGSWAYDIHQLDIIWWVPDGTNVSMPYIDFNDYLQSNEWRTDGEVDFQLKLEGDNKTQIKSVKKYRTRVYVSSNGQPINKHYPXXXXXXXXXXNSSFYVLILVIPCILLSSLTLVVFWLPPESPAKMMLGMNIXXXXXXXXXXXXESTPSAVKKFPLIGAYFCLNMIMITLSTFLAALVINYYFRGDFAGPLPILVRRIIVEGLGRLLLVRQHIPLPDETNLMDRVLTRAENGCIRKCRLKIPNLDSNKFLTSPVQTAEPPLQQDLSPRNTNPFINNDELAESNVWQTNVRPLRSYSSVYRNTPQASMTARPNPITDTEDSPLLMRCNTTLENDVRELKRYIRMFVNRQKENNRKNLISMEWRTLALVLDRLFFVLY 1856
            W++ L   +FL    +T+N    E+ L V +   ++ Y    +PV +S EL + V+  LQL+ L+++DE  Q++ TN W   +WHD  ++WNP  +G I +IRV PD++W PDI L+N AD     + +  VV+ H G  LWVP +I+KS+C +++ +FPFD Q+C L FGSW Y+ +++ + +       V    +D ++Y  S+ W     +D    L   NK   +S  +++ R+                      RR + FY ++L+IP +L++ L++ VF+LP +S  K+ L +++ ++  V LLL+++  P      PL+  Y  L  ++  ++  +  ++IN YFRG     +P  VR + ++ L +L+              M R  + +E   +R    ++P +   +F  SP  +A  PL      R T                             RNT     +A     TD       +R    +E     LKR      + Q ++ R      +W+ +A+++DRL   ++
Sbjct:    8 WILVLSVVIFLV-IINTINASDDEERLMVDV---FRGYNSLIQPVRNSSELPLIVKMALQLVLLINVDEKDQVMHTNVWLTLQWHDFQMKWNPVNYGEIKQIRVSPDKVWLPDIVLFNNADGNYEVSFMCNVVINHKGDMLWVPPAIYKSSCIIDVEFFPFDEQVCTLVFGSWTYNENEIKLEF-------VQAELVDVSEYSASSIWDV---IDVPASLV--NK---RSRIEFQVRI----------------------RRKTLFYTVVLIIPTVLMAFLSMAVFFLPTDSGEKITLTISVLLSIVVFLLLVSKILPPTSSTIPLMAKYLLLTFVLNVITILVTVIIINVYFRGPRTHRMPQWVRVVFLQFLPKLV-------------CMKRPKSASERSAVRSGMAQLPGV--GQFTLSP--SAHHPLCPSADDRTTT---------------------------IRNTASNETSAYYPLSTDA------LRAIDAIEYITEHLKR------DEQHKSFRD-----DWKYVAMIIDRLLLYVF 451

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Query:  222 WMIFLIFGLFLCRGTDTVNWPSVEKNLTVSILNNYQRYGIFGRPVSHSKEL-MKVQYGLQLIQLLDLDENKQILRTNCWSMYRWHDKLLEWNPAEHGGITEIRVFPDQIWTPDIKLYNFXXXXXXXXXXXXXXXKHTGKTLWVPQSIFKSTCAVEITYFPFDTQICMLEFGSWAYDIHQLDIIWWVPDGTNVSMPYIDFNDYLQSNEWRTDGEVDFQLKLEGDNKTQIKSVKKYRTRVYVSSNGQPINKHYPXXXXXXXXXXNSSFYVLILVIPCILLSSLTLVVFWLPPESPAKMMLGMNIXXXXXXXXXXXXESTPSAVKKFPLIGAYFCLNMIMITLSTFLAALVINYYFRGDFAGPLPILVRRIIVEGLGRLLLVRQHIPLPDETNLMDRVLTRAENGCIRKCRLKIPNLDSNKFLTSPVQTAEPPLQQDLSPRNTNPFINNDELAESNVWQTNVRPLRSYSSVYRNTPQASMTARPNPITDTEDSPLLMRCNTTLENDVRELKRYIRMFVNRQKENNRKNLISMEWRTLALVLDRLFFVLY 1856
            W++ L   +FL    +T+N    E+ L V +   ++ Y    +PV +S EL + V+  LQL+ L+++DE  Q++ TN W   +WHD  ++WNP  +G I +IRV PD++W PDI L+N AD     + +  VV+ H G  LWVP +I+KS+C +++ +FPFD Q+C L FGSW Y+ +++ + +       V    +D ++Y  S+ W     +D    L   NK   +S  +++ R+                      RR + FY ++L+IP +L++ L++ VF+LP +S  K+ L +++ ++  V LLL+++  P      PL+  Y  L  ++  ++  +  ++IN YFRG     +P  VR + ++ L +L+              M R  + +E   +R    ++P +   +F  SP  +A  PL      R T                             RNT     +A     TD       +R    +E     LKR      + Q ++ R      +W+ +A+++DRL   ++
Sbjct:    8 WILVLSVVIFLV-IINTINASDDEERLMVDV---FRGYNSLIQPVRNSSELPLIVKMALQLVLLINVDEKDQVMHTNVWLTLQWHDFQMKWNPVNYGEIKQIRVSPDKVWLPDIVLFNNADGNYEVSFMCNVVINHKGDMLWVPPAIYKSSCIIDVEFFPFDEQVCTLVFGSWTYNENEIKLEF-------VQAELVDVSEYSASSIWDV---IDVPASLV--NK---RSRIEFQVRI----------------------RRKTLFYTVVLIIPTVLMAFLSMAVFFLPTDSGEKITLTISVLLSIVVFLLLVSKILPPTSSTIPLMAKYLLLTFVLNVITILVTVIIINVYFRGPRTHRMPQWVRVVFLQFLPKLV-------------CMKRPKSASERSAVRSGMAQLPGV--GQFTLSP--SAHHPLCPSADDRTTT---------------------------IRNTASNETSAYYPLSTDA------LRAIDAIEYITEHLKR------DEQHKSFRD-----DWKYVAMIIDRLLLYVF 451

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BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Celegans
Match: acr-3 (Acetylcholine receptor subunit beta-type acr-3 [Source:UniProtKB/Swiss-Prot;Acc:Q93149])

HSP 1 Score: 177.563 bits (449), Expect = 5.468e-48
Identity = 126/426 (29.58%), Postives = 213/426 (50.00%), Query Frame = 3

Query:  228 IFLIFGLFLCRGTDTVNWP-SVEKNLTVSILNNYQRYGIFGRPV-SHSKELMKVQYGLQLIQLLDLDENKQILRTNCWSMYRWHDKLLEWNPAEHGGITEIRVFPDQIWTPDIKLYNFXXXXXXXXXXXXXXXKHTGKTLWVPQSIFKSTCAVEITYFPFDTQICMLEFGSWAYDIHQLDIIWWVPDGTNVSMPYIDFNDYLQSNEWRTDGEVDFQLKLEGDNKTQIKSVKKYRTRVYVSSNGQPINKHYPXXXXXXXXXXNSSFYVLILVIPCILLSSLTLVVFWLPPESPAKMMLGMNIXXXXXXXXXXXXESTPSAVKKFPLIGAYFCLNMIMITLSTFLAALVINYYFRGDFAGPLPILVRRIIVEGLGRLLLVRQHIPLPDETNLMDRVLTRAENGCIRKCRLKIPNLDSNKFLTSPVQTA 1499
            +F I  +FL   T+   +P S E+ L   I + Y       RPV + S   ++V + L  + L+++DE  QI++TN W   +W+D  ++W+P E  GI  IRV PD++W PDI L+N AD     +  + VVV+HTG  LWVP +++KS+C +++ +FPFD Q+C L FGSW +   +L + +    G      +++ NDYL S  W         +    D +++I    K R +                            FY +IL++P +L++ L+++VF+LP ES  K+ L ++I +A  V LL++++  P      PL+  Y  +  IM  ++  ++ ++IN YFRG     +P  V+ + ++ L  L ++R+  P   E  L      + E   ++         +  K    P QT+
Sbjct:    6 LFSIITIFLI--TELQCYPNSAEERLLSYIFDGYNS---LIRPVLNASSPPIEVFFSLAFVLLINVDEKNQIMQTNVWPTMKWNDYQMQWDPREFDGIKTIRVPPDKVWLPDIVLFNNADGNYLVSFYSNVVVEHTGDMLWVPPAVYKSSCLIDVEFFPFDEQVCSLTFGSWTFRKDELQLSYL--SGKR----HVELNDYLPSGVWDLIDAPGLLI----DERSKISYQIKIRRKAL--------------------------FYTVILIMPTVLMAFLSMMVFYLPAESSEKITLAISILLALVVFLLVVSKILPPTSSTIPLMAKYLLMTFIMNMITIMVSVIIINVYFRGPATHIMPNWVKTVFLKFLPVLFVMRR--PESTEKELAKMKREKRERRSMKSALKTFFKRNDAKISEQPKQTS 388

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BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Celegans
Match: lev-1 (Acetylcholine receptor subunit beta-type lev-1 [Source:UniProtKB/Swiss-Prot;Acc:Q27218])

HSP 1 Score: 174.866 bits (442), Expect = 6.484e-47
Identity = 119/394 (30.20%), Postives = 206/394 (52.28%), Query Frame = 3

Query:  222 WMIFLIFGLFLCRGTDTVNWPSVEKNLTVSILNNYQRYGIFGRPVSHSKEL-MKVQYGLQLIQLLDLDENKQILRTNCWSMYRWHDKLLEWNPAEHGGITEIRVFPDQIWTPDIKLYNFXXXXXXXXXXXXXXXKHTGKTLWVPQSIFKSTCAVEITYFPFDTQICMLEFGSWAYDIHQLDIIWWVPDGTNVSMPYIDFNDYLQSNEWRTDGEVDFQLKLEGDNKTQIKSVKKYRTRVYVSSNGQPINKHYPXXXXXXXXXXNSSFYVLILVIPCILLSSLTLVVFWLPPESPAKMMLGMNIXXXXXXXXXXXXESTPSAVKKFPLIGAYFCLNMIMITLSTFLAALVINYYFRGDFAGPLPILVRRIIVEGLGRLLLVRQHIPLPDETNLMDR 1400
            W+ FL+F     R   T      E  L V +   ++ Y    +PV +  EL M V+ G+QL+ L+++DE +Q++ TN W   +W D  L+W+P ++  IT+IRV P+++W PDI L+N AD     + +  V++  TG  LWVP +I+KS+C +++ +FPFD Q+C L FGSW Y+  ++ + +   D        +DF++Y  S+ W     +D    L  D     +S  +++ R+                      RR + FY ++L++P +L++ L + VF+LP  S  KM L MN+ ++  V LLL+++  P      PL+  Y  L  ++  ++  +  ++ N YFR      LP  VR++ ++ L  L+ +++    P   N++ R
Sbjct:   15 WLGFLVFLAVSLRNHSTCEDIDAEDRLMVDL---FRGYNSLVQPVRNRSELPMIVKIGMQLVLLINVDEKEQVMHTNVWLTMKWDDFQLKWDPRDYANITQIRVAPEKVWLPDIVLFNNADGNYEVSFMCNVLILSTGTVLWVPPAIYKSSCIIDVEFFPFDDQLCSLTFGSWTYNRDEIKLDFLTSD-------RVDFSEYSTSSIWDM---MDGPAVLTSD-----RSRIEFQIRI----------------------RRKTLFYTVVLILPTVLMAFLNVTVFYLPTASGEKMGLTMNVLLSIVVFLLLVSKILPPTSSSIPLVAKYLLLTFVLNIITIMVTTIICNIYFRSPITHRLPPWVRKVFLDILPLLMCMQR----PHRKNVIQR 364

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BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Fly
Match: nAChRalpha2 (gene:FBgn0000039 transcript:FBtr0084639)

HSP 1 Score: 202.601 bits (514), Expect = 4.579e-56
Identity = 161/566 (28.45%), Postives = 270/566 (47.70%), Query Frame = 3

Query:  267 DTVNWPSVEKNLTVSILNNYQRYGIFGRPVSHSKELMKVQYGLQLIQLLDLDENKQILRTNCWSMYRWHDKLLEWNPAEHGGITEIRVFPDQIWTPDIKLYNFXXXXXXXXXXXXXXXKHTGKTLWVPQSIFKSTCAVEITYFPFDTQICMLEFGSWAYDIHQLDI--IWWVPDGTNVSMPYIDFNDYLQSNEWRTDGEVDFQLKLEGDNKTQIKSVKKYRTRVYVSSNGQPINKHYPXXXXXXXXXXNSSFYVLILVIPCILLSSLTLVVFWLPPESPAKMMLGMNIXXXXXXXXXXXXESTPSAVKKFPLIGAYFCLNMIMITLSTFLAALVINYYFRGDFAGPLPILVRRIIVEGLGRLLLVRQHIPLPDETNLMDRVLTRAENGCIRKCRLKIPNLDSNKFLTSPVQT----AEPPLQQDLSPRNTNPFINNDELAESNVWQTNVRPLRSYSSVYRNTPQASMTARPNPITDTEDS----PLLMRCNTTLENDVRELKRYIRMFVNRQKENNRKNLISMEWRTLALVLDRLFFVLYISSILIAVIACIPSSTEPVIPAQYRD 1934
            +TV      K L   +L+NY R     RPVS++ + + V+ GL+L QL+DL+   QIL TN W  + W D   +W+P+E+GG+TE+ V  + IW PDI LYN AD       + + ++ +TGK +W P +IFKS+C +++ YFPFD Q C ++FGSW YD  Q+D+  I    D  N     ID  +Y  S EW                   I  V   R   Y     +P    YP + + + LRR + FY + L+IPC+ +S L+++VF+LP +S  K+ L ++I ++  +  LL++E  PS     PL+G Y    M+++ LS  +  +++N ++R      +   +R   ++ L +LLL+R    +P +  L D    +   G      LK       + L   +Q     + P   + +  R      N   +  +    TN       +     +  +     P+ ++  +DS        +    LE  +  +  +I+  + RQ E N ++    +W  +A+V+DRLF  L++ + L+     +  +     P+ Y D
Sbjct:   37 ETVQANPDAKRLYDDLLSNYNR---LIRPVSNNTDTVLVKLGLRLSQLIDLNLKDQILTTNVWLEHEWQDHKFKWDPSEYGGVTELYVPSEHIWLPDIVLYNNADGEYVVTTMTKAILHYTGKVVWTPPAIFKSSCEIDVRYFPFDQQTCFMKFGSWTYDGDQIDLKHISQKNDKDNKVEIGIDLREYYPSVEW------------------DILGVPAERHEKYYPCCAEP----YPDIFFNITLRRKTLFYTVNLIIPCVGISYLSVLVFYLPADSGEKIALCISILLSQTMFFLLISEIIPSTSLALPLLGKYLLFTMLLVGLSVVITIIILNIHYRKPSTHKMRPWIRSFFIKRLPKLLLMR----VPKDL-LRDLAANKINYG------LKFSKTKFGQALMDEMQMNSGGSSPDSLRRMQGRVGAGGCNGMHVTTA----TNRFSGLVGALGGGLSTLSGYNGLPSVLSGLDDSLSDVAARKKYPFELEKAIHNV-MFIQHHMQRQDEFNAED---QDWGFVAMVMDRLFLWLFMIASLVGTFVILGEA-----PSLYDD 553

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BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Fly
Match: nAChRalpha2 (gene:FBgn0000039 transcript:FBtr0084640)

HSP 1 Score: 202.601 bits (514), Expect = 4.579e-56
Identity = 161/566 (28.45%), Postives = 270/566 (47.70%), Query Frame = 3

Query:  267 DTVNWPSVEKNLTVSILNNYQRYGIFGRPVSHSKELMKVQYGLQLIQLLDLDENKQILRTNCWSMYRWHDKLLEWNPAEHGGITEIRVFPDQIWTPDIKLYNFXXXXXXXXXXXXXXXKHTGKTLWVPQSIFKSTCAVEITYFPFDTQICMLEFGSWAYDIHQLDI--IWWVPDGTNVSMPYIDFNDYLQSNEWRTDGEVDFQLKLEGDNKTQIKSVKKYRTRVYVSSNGQPINKHYPXXXXXXXXXXNSSFYVLILVIPCILLSSLTLVVFWLPPESPAKMMLGMNIXXXXXXXXXXXXESTPSAVKKFPLIGAYFCLNMIMITLSTFLAALVINYYFRGDFAGPLPILVRRIIVEGLGRLLLVRQHIPLPDETNLMDRVLTRAENGCIRKCRLKIPNLDSNKFLTSPVQT----AEPPLQQDLSPRNTNPFINNDELAESNVWQTNVRPLRSYSSVYRNTPQASMTARPNPITDTEDS----PLLMRCNTTLENDVRELKRYIRMFVNRQKENNRKNLISMEWRTLALVLDRLFFVLYISSILIAVIACIPSSTEPVIPAQYRD 1934
            +TV      K L   +L+NY R     RPVS++ + + V+ GL+L QL+DL+   QIL TN W  + W D   +W+P+E+GG+TE+ V  + IW PDI LYN AD       + + ++ +TGK +W P +IFKS+C +++ YFPFD Q C ++FGSW YD  Q+D+  I    D  N     ID  +Y  S EW                   I  V   R   Y     +P    YP + + + LRR + FY + L+IPC+ +S L+++VF+LP +S  K+ L ++I ++  +  LL++E  PS     PL+G Y    M+++ LS  +  +++N ++R      +   +R   ++ L +LLL+R    +P +  L D    +   G      LK       + L   +Q     + P   + +  R      N   +  +    TN       +     +  +     P+ ++  +DS        +    LE  +  +  +I+  + RQ E N ++    +W  +A+V+DRLF  L++ + L+     +  +     P+ Y D
Sbjct:   37 ETVQANPDAKRLYDDLLSNYNR---LIRPVSNNTDTVLVKLGLRLSQLIDLNLKDQILTTNVWLEHEWQDHKFKWDPSEYGGVTELYVPSEHIWLPDIVLYNNADGEYVVTTMTKAILHYTGKVVWTPPAIFKSSCEIDVRYFPFDQQTCFMKFGSWTYDGDQIDLKHISQKNDKDNKVEIGIDLREYYPSVEW------------------DILGVPAERHEKYYPCCAEP----YPDIFFNITLRRKTLFYTVNLIIPCVGISYLSVLVFYLPADSGEKIALCISILLSQTMFFLLISEIIPSTSLALPLLGKYLLFTMLLVGLSVVITIIILNIHYRKPSTHKMRPWIRSFFIKRLPKLLLMR----VPKDL-LRDLAANKINYG------LKFSKTKFGQALMDEMQMNSGGSSPDSLRRMQGRVGAGGCNGMHVTTA----TNRFSGLVGALGGGLSTLSGYNGLPSVLSGLDDSLSDVAARKKYPFELEKAIHNV-MFIQHHMQRQDEFNAED---QDWGFVAMVMDRLFLWLFMIASLVGTFVILGEA-----PSLYDD 553

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BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Fly
Match: nAChRalpha6 (gene:FBgn0032151 transcript:FBtr0331284)

HSP 1 Score: 197.593 bits (501), Expect = 5.122e-55
Identity = 156/542 (28.78%), Postives = 264/542 (48.71%), Query Frame = 3

Query:  291 EKNLTVSILNNYQRYGIFGRPVSHSKELMKVQYGLQLIQLLDLDENKQILRTNCWSMYRWHDKLLEWNPAEHGGITEIRVFPDQIWTPDIKLYNFXXXXXXXXXXXXXXXKHTGKTLWVPQSIFKSTCAVEITYFPFDTQICMLEFGSWAYDIHQLDIIWWVPDGTNVSMPYIDFNDYLQSNEWRTDGEVDFQLKLEGDNKTQIKSVKKYRTRVYVSSNGQPINKHYPXXXXXXXXXXNSSFYVLILVIPCILLSSLTLVVFWLPPESPAKMMLGMNIXXXXXXXXXXXXESTPSAVKKFPLIGAYFCLNMIMITLSTFLAALVINYYFRGDFAGPLPILVRRIIVEGLGRLLLVRQHIPLPDETNLMDRVLTRAENGCIRKCRLKIPNLDSNKFLTSPVQTAEPPLQQDLSPRNTNPFINNDELAESNVWQTNVRPLRSYSSVYRNTPQASMTARPNPITDTEDSPLLMRCNTTLENDVRELKRYIRMFVNRQKENNRKNLISMEWRTLALVLDRLFFVLYISSILIAVIACIPSSTEPVI 1916
            EK L   +L+ Y       RPV++  E ++V++GL L Q++D+DE  QIL TN W    W+D  L WN  E+GG+ ++R+ P+++W PD+ +YN AD          +VVKH G  L+VP  IFKSTC ++IT+FPFD Q C ++FGSW YD +QLD++    DG        D +D++ + EW       + L + G          K  T VY     +P    Y  + + +++RR + +Y   L++PC+L+SS+ L+ F LPP+S  K+ LG+ I ++  V L L+AES P+     PLIG YF   M M+  S  L  +V+NY+ R      +P  ++ + ++ L  +L  R   P         R +TR     +   R+K                     + +L  R++   +       +NV   +     + S        ++   RP   T  E+    + CN     D+  + + ++    R ++ + +  +  +W+  A+V+DR   +++    +IA +  + S+   ++
Sbjct:   30 EKRLLNHLLSTYNT---LERPVANESEPLEVKFGLTLQQIIDVDEKNQILTTNAWLNLEWNDYNLRWNETEYGGVKDLRITPNKLWKPDVLMYNSADEGFDGTYHTNIVVKHNGSCLYVPPGIFKSTCKIDITWFPFDDQHCEMKFGSWTYDGNQLDLVLNSEDGG-------DLSDFITNGEW-------YLLAMPG----------KKNTIVYACC-PEP----YVDITFTIQIRRRTLYYFFNLIVPCVLISSMALLGFTLPPDSGEKLTLGVTILLSLTVFLNLVAESMPTTSDAVPLIGTYFNCIMFMVASSVVLTVVVLNYHHRTADIHEMPPWIKSVFLQWLPWIL--RMGRP--------GRKITRKT--ILLSNRMK---------------------ELELKERSSKSLL-------ANVLDIDDDFRHTISGSQTAIGSSASFGRP---TTVEEHHTAIGCN---HKDLHLILKELQFITARMRKADDEAELIGDWKFAAMVVDRFCLIVFTLFTIIATVTVLLSAPHIIV 493

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BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Fly
Match: nAChRalpha6 (gene:FBgn0032151 transcript:FBtr0303898)

HSP 1 Score: 195.667 bits (496), Expect = 2.711e-54
Identity = 155/542 (28.60%), Postives = 264/542 (48.71%), Query Frame = 3

Query:  291 EKNLTVSILNNYQRYGIFGRPVSHSKELMKVQYGLQLIQLLDLDENKQILRTNCWSMYRWHDKLLEWNPAEHGGITEIRVFPDQIWTPDIKLYNFXXXXXXXXXXXXXXXKHTGKTLWVPQSIFKSTCAVEITYFPFDTQICMLEFGSWAYDIHQLDIIWWVPDGTNVSMPYIDFNDYLQSNEWRTDGEVDFQLKLEGDNKTQIKSVKKYRTRVYVSSNGQPINKHYPXXXXXXXXXXNSSFYVLILVIPCILLSSLTLVVFWLPPESPAKMMLGMNIXXXXXXXXXXXXESTPSAVKKFPLIGAYFCLNMIMITLSTFLAALVINYYFRGDFAGPLPILVRRIIVEGLGRLLLVRQHIPLPDETNLMDRVLTRAENGCIRKCRLKIPNLDSNKFLTSPVQTAEPPLQQDLSPRNTNPFINNDELAESNVWQTNVRPLRSYSSVYRNTPQASMTARPNPITDTEDSPLLMRCNTTLENDVRELKRYIRMFVNRQKENNRKNLISMEWRTLALVLDRLFFVLYISSILIAVIACIPSSTEPVI 1916
            EK L   +L+ Y       RPV++  E ++V++GL L Q++D+DE  Q+L TN W    W+D  L WN  E+GG+ ++R+ P+++W PD+ +YN AD          +VVKH G  L+VP  IFKSTC ++IT+FPFD Q C ++FGSW YD +QLD++    DG        D +D++ + EW       + L + G          K  T VY     +P    Y  + + +++RR + +Y   L++PC+L+SS+ L+ F LPP+S  K+ LG+ I ++  V L L+AES P+     PLIG YF   M M+  S  L  +V+NY+ R      +P  ++ + ++ L  +L  R   P         R +TR     +   R+K                     + +L  R++   +       +NV   +     + S        ++   RP   T  E+    + CN     D+  + + ++    R ++ + +  +  +W+  A+V+DR   +++    +IA +  + S+   ++
Sbjct:   30 EKRLLNHLLSTYNT---LERPVANESEPLEVKFGLTLQQIIDVDEKNQLLITNLWLSLEWNDYNLRWNETEYGGVKDLRITPNKLWKPDVLMYNSADEGFDGTYHTNIVVKHNGSCLYVPPGIFKSTCKIDITWFPFDDQHCEMKFGSWTYDGNQLDLVLNSEDGG-------DLSDFITNGEW-------YLLAMPG----------KKNTIVYACC-PEP----YVDITFTIQIRRRTLYYFFNLIVPCVLISSMALLGFTLPPDSGEKLTLGVTILLSLTVFLNLVAESMPTTSDAVPLIGTYFNCIMFMVASSVVLTVVVLNYHHRTADIHEMPPWIKSVFLQWLPWIL--RMGRP--------GRKITRKT--ILLSNRMK---------------------ELELKERSSKSLL-------ANVLDIDDDFRHTISGSQTAIGSSASFGRP---TTVEEHHTAIGCN---HKDLHLILKELQFITARMRKADDEAELIGDWKFAAMVVDRFCLIVFTLFTIIATVTVLLSAPHIIV 493

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BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Fly
Match: nAChRalpha6 (gene:FBgn0032151 transcript:FBtr0079916)

HSP 1 Score: 195.667 bits (496), Expect = 2.711e-54
Identity = 155/542 (28.60%), Postives = 264/542 (48.71%), Query Frame = 3

Query:  291 EKNLTVSILNNYQRYGIFGRPVSHSKELMKVQYGLQLIQLLDLDENKQILRTNCWSMYRWHDKLLEWNPAEHGGITEIRVFPDQIWTPDIKLYNFXXXXXXXXXXXXXXXKHTGKTLWVPQSIFKSTCAVEITYFPFDTQICMLEFGSWAYDIHQLDIIWWVPDGTNVSMPYIDFNDYLQSNEWRTDGEVDFQLKLEGDNKTQIKSVKKYRTRVYVSSNGQPINKHYPXXXXXXXXXXNSSFYVLILVIPCILLSSLTLVVFWLPPESPAKMMLGMNIXXXXXXXXXXXXESTPSAVKKFPLIGAYFCLNMIMITLSTFLAALVINYYFRGDFAGPLPILVRRIIVEGLGRLLLVRQHIPLPDETNLMDRVLTRAENGCIRKCRLKIPNLDSNKFLTSPVQTAEPPLQQDLSPRNTNPFINNDELAESNVWQTNVRPLRSYSSVYRNTPQASMTARPNPITDTEDSPLLMRCNTTLENDVRELKRYIRMFVNRQKENNRKNLISMEWRTLALVLDRLFFVLYISSILIAVIACIPSSTEPVI 1916
            EK L   +L+ Y       RPV++  E ++V++GL L Q++D+DE  Q+L TN W    W+D  L WN  E+GG+ ++R+ P+++W PD+ +YN AD          +VVKH G  L+VP  IFKSTC ++IT+FPFD Q C ++FGSW YD +QLD++    DG        D +D++ + EW       + L + G          K  T VY     +P    Y  + + +++RR + +Y   L++PC+L+SS+ L+ F LPP+S  K+ LG+ I ++  V L L+AES P+     PLIG YF   M M+  S  L  +V+NY+ R      +P  ++ + ++ L  +L  R   P         R +TR     +   R+K                     + +L  R++   +       +NV   +     + S        ++   RP   T  E+    + CN     D+  + + ++    R ++ + +  +  +W+  A+V+DR   +++    +IA +  + S+   ++
Sbjct:   30 EKRLLNHLLSTYNT---LERPVANESEPLEVKFGLTLQQIIDVDEKNQLLITNLWLSLEWNDYNLRWNETEYGGVKDLRITPNKLWKPDVLMYNSADEGFDGTYHTNIVVKHNGSCLYVPPGIFKSTCKIDITWFPFDDQHCEMKFGSWTYDGNQLDLVLNSEDGG-------DLSDFITNGEW-------YLLAMPG----------KKNTIVYACC-PEP----YVDITFTIQIRRRTLYYFFNLIVPCVLISSMALLGFTLPPDSGEKLTLGVTILLSLTVFLNLVAESMPTTSDAVPLIGTYFNCIMFMVASSVVLTVVVLNYHHRTADIHEMPPWIKSVFLQWLPWIL--RMGRP--------GRKITRKT--ILLSNRMK---------------------ELELKERSSKSLL-------ANVLDIDDDFRHTISGSQTAIGSSASFGRP---TTVEEHHTAIGCN---HKDLHLILKELQFITARMRKADDEAELIGDWKFAAMVVDRFCLIVFTLFTIIATVTVLLSAPHIIV 493

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BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Zebrafish
Match: chrna2a (cholinergic receptor, nicotinic, alpha 2a (neuronal) [Source:ZFIN;Acc:ZDB-GENE-040108-2])

HSP 1 Score: 204.142 bits (518), Expect = 5.527e-57
Identity = 155/524 (29.58%), Postives = 251/524 (47.90%), Query Frame = 3

Query:  333 YGIFGRPVSHSKELMKVQYGLQLIQLLDLDENKQILRTNCWSMYRWHDKLLEWNPAEHGGITEIRVFPDQIWTPDIKLYNFXXXXXXXXXXXXXXXKHTGKTLWVPQSIFKSTCAVEITYFPFDTQICMLEFGSWAYDIHQLDIIWWVPDGTNVSMPYIDFNDYLQSNEWRTDGEVDFQLKLEGDNKTQIKSVKKYRTRVYVSSNGQPINKHYPXXXXXXXXXXNSSFYVLILVIPCILLSSLTLVVFWLPPESPAKMMLGMNIXXXXXXXXXXXXESTPSAVKKFPLIGAYFCLNMIMITLSTFLAALVINYYFRGDFAGPLPILVRRIIVEGLGRLLLVRQHIPLPDETNLMDRVLTRAENGCIRKCRLKIPNLDSNKFLTSPVQTAEPPLQQD------LSPRNTNPFINNDELAESNVWQTNVRPLRSYSSVYRNTPQASMTARPNPITDTEDSPLLMRCNTTLENDVRELKRYIRMFVNRQKENNRKNLISMEWRTLALVLDRLFFVLYISSILIAVIA 1886
            Y  + RPV +  +++ V++GL + QL+D+DE  Q++ TN W    W+D  L WNP+E   +T IRV  + IW PDI LYN AD       + +  + HTGK  WVP +I+KS+C++++T+FPFD Q C ++FGSW YD  ++D+     +GT      +D  DY +S EW                   I +V  Y T+ Y        ++ YP + Y   +RR   FY + L+IPC+L+S LT++VF+LP +   K+ L +++ ++  V LLL+ E  PS     PLIG Y    MI +TLS  +   V+N + R      +P  V  + ++ + R L +R+  P P+                  K   K  NL ++K         +  + QD      LS   ++P   +  L++S        PL     +       ++   P  + +    P L+     L     E  RYI   +  + E+     +  +W+ +A+V+DR+F  ++I   L+  I 
Sbjct:   48 YNKWSRPVRNISDVVIVKFGLSIAQLIDVDEKNQMMTTNVWLKQEWNDYKLRWNPSEFDNVTSIRVPSEMIWVPDIVLYNNADGEFAVTHMTKAHLFHTGKVSWVPPAIYKSSCSIDVTFFPFDQQNCKMKFGSWTYDKAKIDLE--QIEGT------VDLKDYWESGEWAI-----------------INAVGTYNTKKY-----DCCHEIYPDITYFFIIRRLPLFYTINLIIPCLLISCLTVLVFYLPSDCGEKITLCISVLLSLTVFLLLITEIIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHHRSPSTHSMPRWVHSVFLDHIPRWLFMRR--PEPE------------------KKPKKTANLSTSKSWFQQETGVDGLVCQDMEFGIGLSTSISSPSATSLHLSDS-------EPLLQKYELQHLGHVVNLCQHPAKLGNLVSEPGLLFSPCVLR--ALEGVRYIADHLRAEDEDFS---VKEDWKYVAMVIDRIFLWMFIIVCLLGTIG 509

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BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Zebrafish
Match: chrna2b (cholinergic receptor, nicotinic, alpha 2b (neuronal) [Source:ZFIN;Acc:ZDB-GENE-041001-99])

HSP 1 Score: 196.438 bits (498), Expect = 5.538e-54
Identity = 120/353 (33.99%), Postives = 190/353 (53.82%), Query Frame = 3

Query:  324 YQRYGIFGRPVSHSKELMKVQYGLQLIQLLDLDENKQILRTNCWSMYRWHDKLLEWNPAEHGGITEIRVFPDQIWTPDIKLYNFXXXXXXXXXXXXXXXKHTGKTLWVPQSIFKSTCAVEITYFPFDTQICMLEFGSWAYDIHQLDIIWWVPDGTNVSMPYIDFNDYLQSNEWRTDGEVDFQLKLEGDNKTQIKSVKKYRTRVYVSSNGQPINKHYPXXXXXXXXXXNSSFYVLILVIPCILLSSLTLVVFWLPPESPAKMMLGMNIXXXXXXXXXXXXESTPSAVKKFPLIGAYFCLNMIMITLSTFLAALVINYYFRGDFAGPLPILVRRIIVEGLGRLLLVRQHIPLPDE 1382
            +  Y  + RPV ++ +++ V++GL + QL+D+DE  Q++ TN W    W+D  L W P+++  +T IRV  + IW PDI LYN AD       + +  + HTGK  WVP +I+KS+C++++T+FPFD Q C ++FGSW YD  ++D+              +D  DY +S EW                   I +V  Y T+ Y        ++ YP + Y   +RR   FY + L+IPC+L+S LT++VF+LP +   K+ L +++ ++  V LLL+ E  PS     PLIG Y    MI +TLS  +   V+N + R      +P  V  + ++ + R L +R+  P PD 
Sbjct:   43 FDGYNKWSRPVPNTSDVVIVKFGLSIAQLIDVDEKNQMMTTNVWLKQEWNDYKLRWKPSDYDNVTSIRVPSELIWVPDIVLYNNADGEFAVTHMTKAHLFHTGKVRWVPPAIYKSSCSIDVTFFPFDQQNCKMKFGSWTYDKAKIDL--------ERIENTVDLKDYWESGEWAI-----------------INAVGTYNTKKY-----DCCHEIYPDITYFFIIRRLPLFYTINLIIPCLLISCLTVLVFYLPSDCGEKITLCISVLLSLTVFLLLITEIIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHHRSPSTHKMPCWVHSVFLDLIPRWLFMRR--PAPDS 363

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BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Zebrafish
Match: chrna3 (cholinergic receptor, nicotinic, alpha 3 [Source:ZFIN;Acc:ZDB-GENE-070822-1])

HSP 1 Score: 194.897 bits (494), Expect = 1.960e-53
Identity = 137/536 (25.56%), Postives = 254/536 (47.39%), Query Frame = 3

Query:  252 LCRGTDTVNWPSVEKNLTVSILNNYQRYGIFGRPVSHSKELMKVQYGLQLIQLLDLDENKQILRTNCWSMYRWHDKLLEWNPAEHGGITEIRVFPDQIWTPDIKLYNFXXXXXXXXXXXXXXXKHTGKTLWVPQSIFKSTCAVEITYFPFDTQICMLEFGSWAYDIHQLDIIWWVPDGTNVSMPYIDFNDYLQSNEWRTDGEVDFQLKLEGDNKTQIKSVKKYRTRVYVSSNGQPINKHYPXXXXXXXXXXNSSFYVLILVIPCILLSSLTLVVFWLPPESPAKMMLGMNIXXXXXXXXXXXXESTPSAVKKFPLIGAYFCLNMIMITLSTFLAALVINYYFRGDFAGPLPILVRRIIVEGLGRLLLVRQHIPLPDETNLMDRVLTRAENGCIRKCRLKIPNLDSNKFLTSPVQTAEPPLQQDLSPRNTNPFINNDELAESNVWQTNVRPLRSYSSVYRNTPQASMTARPNPITDTEDSPLLMRCNTTLENDVRELKRYIRMFVNRQKENNRKNLISMEWRTLALVLDRLFFVLYI 1859
            LC     V     E  L   I ++Y +Y    RPV +  + + VQ+ + + QL+ +DE  QI+ TN W  + W+D  L W+P + GG+  IRV  ++IW PDI LYN A    +     + +++  G   W+P +IFKS+C +++TYFPFD Q C ++FGSW YD  ++D++        +    I+  D+ +S EW       ++  ++ +   +I +   Y   +          +  P+            FY + ++IPC+L+S LT++VF+LP +   K+ L +++ ++  V LL++ E+ PS     PLIG Y    MI +TLS  +   V+N ++R      +P  VRR+ +  L R++ + +    P++   +            ++C +  P       +    Q    P + + + R    F+      E + W+ ++  + S  +    +P +S        +++ D  L   C + +   VRE    ++      +  N    +  +W+ +A+V+DR+F  +++
Sbjct:   51 LCFLLSGVCCSEAEHRLFSVIFSSYNQYI---RPVENVSDPVVVQFEVSMSQLVKVDEVNQIMETNLWLRHIWNDYKLRWDPKDFGGVEFIRVPSNKIWKPDIVLYNNAVGDFQVDDKTKALLRFNGDVTWIPPAIFKSSCKIDVTYFPFDYQNCTMKFGSWTYDKAKIDLV--------LIGSTINLKDFWESGEWTIIDAPGYKHDIKYNCCEEIYTDITYSLYI----------RRLPL------------FYTINMIIPCLLISFLTVLVFYLPSDCGEKVTLCISVLLSLTVFLLVITETIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHYRTPKTHTMPCWVRRVFLSLLPRVMFMTR----PEKDQEVPA----------KQCSVHPPVSSKQTSVCRGPQLLLCPTELNEASRTA--FLCR----EHHCWKKHISNMTSEGAEDGGSPCSS--------SESLDGFL---CMSAVSPQVREAIESVKYIAENMRLQNEAKEVQDDWKYVAMVIDRIFLWVFV 522

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BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Zebrafish
Match: chrnb3a (cholinergic receptor, nicotinic, beta polypeptide 3a [Source:ZFIN;Acc:ZDB-GENE-040108-1])

HSP 1 Score: 191.815 bits (486), Expect = 5.157e-53
Identity = 123/365 (33.70%), Postives = 189/365 (51.78%), Query Frame = 3

Query:  273 VNWPSVEKNLTVSILNNYQRYGIFGRPVSHSKELMKVQYGLQLIQLLDLDENKQILRTNCWSMYRWHDKLLEWNPAEHGGITEIRVFPDQIWTPDIKLYNFXXXXXXXXXXXXXXXKHTGKTLWVPQSIFKSTCAVEITYFPFDTQICMLEFGSWAYDIHQLDIIWWVPDGTNVSMPYIDFNDYLQSNEWRTDGEVDFQLKLEGDNKTQIKSVKKYRTRVYVSSNGQPINKHYPXXXXXXXXXXNSSFYVLILVIPCILLSSLTLVVFWLPPESPAKMMLGMNIXXXXXXXXXXXXESTPSAVKKFPLIGAYFCLNMIMITLSTFLAALVINYYFRGDFA-GPLPILVRRIIVEGLGRLLLVRQH 1364
            V+   +E  L  ++   YQ++    RPV H+ + + V++GL++ QL+D+DE  Q++ TN W    W D  L WNP ++GGIT IRV  + IW PDI LY  AD R   + + + +V+  G  +W P + +KS C +++T+FPFD Q C ++FGSW YD   +D+         +   Y+D  D+  + EW         L   G   ++   +  Y    Y       I K  P+            FY L L+IPC+ LS LT++VF+LP +   K+ L  ++ V+  V LL++ E  PS+ K  PLIG Y    MI +T S  +   VIN + R      P+   V+ + ++ L RLL +R H
Sbjct:   26 VSLAEMEDTLLRNLFRGYQKWV---RPVLHANDTITVRFGLKISQLVDVDEKNQLMTTNVWLWEEWTDYKLRWNPEDYGGITSIRVPSETIWLPDIVLYENADGRFEGSLMTKAIVRFNGTIMWTPPASYKSACTMDVTFFPFDRQNCSMKFGSWTYDGTMVDL--------TLVDAYVDRKDFFDNGEWEI-------LNATGQRGSRRDGIYSYPYLTY-----SFILKRLPL------------FYTLFLIIPCLGLSFLTVLVFYLPSDEGEKLSLSTSVLVSLTVFLLVIEEIIPSSSKVIPLIGEYLLFIMIFVTFSIIVTVFVINVHHRSSATYHPMAPWVKSLFLQRLPRLLCMRGH 355

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BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Zebrafish
Match: chrnb3a (cholinergic receptor, nicotinic, beta polypeptide 3a [Source:ZFIN;Acc:ZDB-GENE-040108-1])

HSP 1 Score: 191.43 bits (485), Expect = 5.722e-53
Identity = 123/365 (33.70%), Postives = 189/365 (51.78%), Query Frame = 3

Query:  273 VNWPSVEKNLTVSILNNYQRYGIFGRPVSHSKELMKVQYGLQLIQLLDLDENKQILRTNCWSMYRWHDKLLEWNPAEHGGITEIRVFPDQIWTPDIKLYNFXXXXXXXXXXXXXXXKHTGKTLWVPQSIFKSTCAVEITYFPFDTQICMLEFGSWAYDIHQLDIIWWVPDGTNVSMPYIDFNDYLQSNEWRTDGEVDFQLKLEGDNKTQIKSVKKYRTRVYVSSNGQPINKHYPXXXXXXXXXXNSSFYVLILVIPCILLSSLTLVVFWLPPESPAKMMLGMNIXXXXXXXXXXXXESTPSAVKKFPLIGAYFCLNMIMITLSTFLAALVINYYFRGDFA-GPLPILVRRIIVEGLGRLLLVRQH 1364
            V+   +E  L  ++   YQ++    RPV H+ + + V++GL++ QL+D+DE  Q++ TN W    W D  L WNP ++GGIT IRV  + IW PDI LY  AD R   + + + +V+  G  +W P + +KS C +++T+FPFD Q C ++FGSW YD   +D+         +   Y+D  D+  + EW         L   G   ++   +  Y    Y       I K  P+            FY L L+IPC+ LS LT++VF+LP +   K+ L  ++ V+  V LL++ E  PS+ K  PLIG Y    MI +T S  +   VIN + R      P+   V+ + ++ L RLL +R H
Sbjct:   18 VSLAEMEDTLLRNLFRGYQKWV---RPVLHANDTITVRFGLKISQLVDVDEKNQLMTTNVWLWEEWTDYKLRWNPEDYGGITSIRVPSETIWLPDIVLYENADGRFEGSLMTKAIVRFNGTIMWTPPASYKSACTMDVTFFPFDRQNCSMKFGSWTYDGTMVDL--------TLVDAYVDRKDFFDNGEWEI-------LNATGQRGSRRDGIYSYPYLTY-----SFILKRLPL------------FYTLFLIIPCLGLSFLTVLVFYLPSDEGEKLSLSTSVLVSLTVFLLVIEEIIPSSSKVIPLIGEYLLFIMIFVTFSIIVTVFVINVHHRSSATYHPMAPWVKSLFLQRLPRLLCMRGH 347

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BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Xenopus
Match: CHRNA2 (cholinergic receptor, nicotinic alpha 2 [Source:NCBI gene;Acc:100495873])

HSP 1 Score: 204.912 bits (520), Expect = 2.947e-57
Identity = 152/541 (28.10%), Postives = 261/541 (48.24%), Query Frame = 3

Query:  288 VEKNLTVSILNNYQRYGIFGRPVSHSKELMKVQYGLQLIQLLDLDENKQILRTNCWSMYRWHDKLLEWNPAEHGGITEIRVFPDQIWTPDIKLYNFXXXXXXXXXXXXXXXKHTGKTLWVPQSIFKSTCAVEITYFPFDTQICMLEFGSWAYDIHQLDIIWWVPDGTNVSMPY-IDFNDYLQSNEWRTDGEVDFQLKLEGDNKTQIKSVKKYRTRVYVSSNGQPINKHYPXXXXXXXXXXNSSFYVLILVIPCILLSSLTLVVFWLPPESPAKMMLGMNIXXXXXXXXXXXXESTPSAVKKFPLIGAYFCLNMIMITLSTFLAALVINYYFRGDFAGPLPILVRRIIVEGLGRLLLVRQHIPLPDETNLMDRVLTRAENGCIRKCRLKIPNLDSNKFLTSPVQTAEPPLQQDLSPRNTNPFINNDELAESNVWQTNVRPLRS------YSSVYRNTPQASMTARP-NPITDTEDSPLLMRCNTTLENDVRELKRYIRMFVNRQKENNRKNLISMEWRTLALVLDRLFFVLYISSILIAVIA 1886
             E+ L   +   Y R+    RPV ++ +++ V++GL + QL+D+DE  Q++ TN W    W+D  L WNPA++  +T IRV  + IW PDI LYN AD       + +  + H GK  WVP +I+KS+C++++T+FPFD Q C ++FGSW YD   LD+          SM   +D  DY +S EW                   + +V KY ++ Y         + YP + Y   +RR   FY + L+IPC+L+S LT++VF+LP +   K+ L +++ ++  V LLL+ E  PS     PLIG Y    MI +TLS  +   V+N + R      +P+ VR + ++ + R L +++  P P     ++ V+++ +           P L         + T++  ++ D+  +   P        E+ +   +VR + +      Y   Y +      +  P +  T+T +S  L      L   V +    +    N  +  +    +  +W+ +A+V+DR+F  ++I   L+  + 
Sbjct:   31 AEERLFKRLFTGYNRWS---RPVPNTSDVVIVKFGLSIAQLIDVDEKNQMMTTNVWLKQEWNDYKLRWNPADYDNVTSIRVPSEMIWIPDIVLYNNADGEFAITHMTKAHLFHNGKVKWVPPAIYKSSCSIDVTFFPFDQQSCKMKFGSWTYDKAWLDL---------ESMERNVDLKDYWESGEWAI-----------------VNAVGKYNSKKY-----DCCTEIYPDITYYFIIRRLPLFYTINLIIPCLLISCLTVLVFYLPSDCGEKITLCISVLLSLTVFLLLITEIIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHHRSPSTHKMPLWVRSVFLDHIPRWLFMKRP-PAP-----VEEVVSQYDA----------PELK--------LSTSKYWMETDVDDKWAEP--------EAELQTCHVRHMTTHNQSLQYRYDYNHHVSGGQSQTPRHSSTETAESSFL------LSPSVLKALEGVHYIANHLRAEDADFSVKEDWKYVAMVIDRIFLWMFIIVCLLGTVG 499

[Close]

BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Xenopus
Match: CHRNA2 (cholinergic receptor, nicotinic alpha 2 [Source:NCBI gene;Acc:100495873])

HSP 1 Score: 199.904 bits (507), Expect = 1.126e-55
Identity = 126/366 (34.43%), Postives = 197/366 (53.83%), Query Frame = 3

Query:  288 VEKNLTVSILNNYQRYGIFGRPVSHSKELMKVQYGLQLIQLLDLDENKQILRTNCWSMYRWHDKLLEWNPAEHGGITEIRVFPDQIWTPDIKLYNFXXXXXXXXXXXXXXXKHTGKTLWVPQSIFKSTCAVEITYFPFDTQICMLEFGSWAYDIHQLDIIWWVPDGTNVSMPY-IDFNDYLQSNEWRTDGEVDFQLKLEGDNKTQIKSVKKYRTRVYVSSNGQPINKHYPXXXXXXXXXXNSSFYVLILVIPCILLSSLTLVVFWLPPESPAKMMLGMNIXXXXXXXXXXXXESTPSAVKKFPLIGAYFCLNMIMITLSTFLAALVINYYFRGDFAGPLPILVRRIIVEGLGRLLLVRQHIPLPDE 1382
             E+ L   +   Y R+    RPV ++ +++ V++GL + QL+D+DE  Q++ TN W    W+D  L WNPA++  +T IRV  + IW PDI LYN AD       + +  + H GK  WVP +I+KS+C++++T+FPFD Q C ++FGSW YD   LD+          SM   +D  DY +S EW                   + +V KY ++ Y         + YP + Y   +RR   FY + L+IPC+L+S LT++VF+LP +   K+ L +++ ++  V LLL+ E  PS     PLIG Y    MI +TLS  +   V+N + R      +P+ VR + ++ + R L +++  P P E
Sbjct:   31 AEERLFKRLFTGYNRWS---RPVPNTSDVVIVKFGLSIAQLIDVDEKNQMMTTNVWLKQEWNDYKLRWNPADYDNVTSIRVPSEMIWIPDIVLYNNADGEFAITHMTKAHLFHNGKVKWVPPAIYKSSCSIDVTFFPFDQQSCKMKFGSWTYDKAWLDL---------ESMERNVDLKDYWESGEWAI-----------------VNAVGKYNSKKY-----DCCTEIYPDITYYFIIRRLPLFYTINLIIPCLLISCLTVLVFYLPSDCGEKITLCISVLLSLTVFLLLITEIIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHHRSPSTHKMPLWVRSVFLDHIPRWLFMKRP-PAPVE 361

[Close]

BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Xenopus
Match: rpap1 (RNA polymerase II associated protein 1 [Source:Xenbase;Acc:XB-GENE-976775])

HSP 1 Score: 191.43 bits (485), Expect = 5.602e-53
Identity = 136/398 (34.17%), Postives = 195/398 (48.99%), Query Frame = 3

Query:  246 LFLCRGTDTVNWPSVEKNLTVSILNNYQRYGIFGRPVSHSKELMKVQYGLQLIQLLDLDENKQILRTNCWSMYRWHDKLLEWNPAEHGGITEIRVFPDQIWTPDIKLYNFXXXXXXXXXXXXXXXKHTGKTLWVPQSIFKSTCAVEITYFPFDTQICMLEFGSWAYDIHQLDIIWWVPDGTNVSMPYIDFNDYLQSNEWRTDGEVDFQLKLEGDNKTQIKSVKKYRTRVYVSSNGQPINKHYPXXXXXXXXXXNSSFYVLILVIPCILLSSLTLVVFWLPPESPAKMMLGMNIXXXXXXXXXXXXESTPSAVKKFPLIGAYFCLNMIMITLSTFLAALVINYYFRGDFA-GPLPILVRRIIVEGLGRLLLVRQHIPLPDETNLMDRVLTRA-ENGCIR 1433
            +F+  G    ++ +  ++     L   Q Y  + RPV H  + +KV++GL + QL+D+DE  Q++ TN W    W D  L W+P E  GIT IRV  D IW PDI LY+       E  V + VV++ G   W P + +KS+C +++T+FPFD Q C ++FGSW YD  Q+DII        +   ++D  D+  + EW            E  N T  K  +      Y       I K  P+            FY L L+IPCI LS LT++VF+LP     K+ L  ++ V+  V LL++ E  PS+ K  PLIG Y    MI +TLS  L    IN + R       +   VR+I +  L +LL +R H+         DR  TR  E G +R
Sbjct:    1 MFMEHGISEPSYIAKSEDRLFKYLFQQQDYQRWVRPVEHLNDTVKVKFGLAITQLVDVDEKNQLMTTNVWLKQEWVDVKLRWDPNEFAGITSIRVPSDSIWIPDIVLYD-NADGRFEGSVTKAVVRYDGTINWTPPANYKSSCTIDVTFFPFDLQNCSMKFGSWTYDGSQVDII--------LEDYHVDKRDFFDNGEW------------EIVNATGNKGNRTDGCCFYPYITYSFIIKRLPL------------FYTLFLIIPCIGLSFLTILVFYLPSNEGEKISLCTSVLVSLTVFLLVIEEIIPSSSKVIPLIGEYLVFTMIFVTLSIVLTVFAINIHNRSSATHNAMAPWVRKIFLHKLPKLLCMRSHV---------DRYFTRKDETGKVR 356

[Close]

BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Xenopus
Match: rpap1 (RNA polymerase II associated protein 1 [Source:Xenbase;Acc:XB-GENE-976775])

HSP 1 Score: 190.66 bits (483), Expect = 7.715e-53
Identity = 134/374 (35.83%), Postives = 187/374 (50.00%), Query Frame = 3

Query:  318 NNYQRYGIFGRPVSHSKELMKVQYGLQLIQLLDLDENKQILRTNCWSMYRWHDKLLEWNPAEHGGITEIRVFPDQIWTPDIKLYNFXXXXXXXXXXXXXXXKHTGKTLWVPQSIFKSTCAVEITYFPFDTQICMLEFGSWAYDIHQLDIIWWVPDGTNVSMPYIDFNDYLQSNEWRTDGEVDFQLKLEGDNKTQIKSVKKYRTRVYVSSNGQPINKHYPXXXXXXXXXXNSSFYVLILVIPCILLSSLTLVVFWLPPESPAKMMLGMNIXXXXXXXXXXXXESTPSAVKKFPLIGAYFCLNMIMITLSTFLAALVINYYFRGDFA-GPLPILVRRIIVEGLGRLLLVRQHIPLPDETNLMDRVLTRA-ENGCIR 1433
             +YQR+    RPV H  + +KV++GL + QL+D+DE  Q++ TN W    W D  L W+P E  GIT IRV  D IW PDI LY+       E  V + VV++ G   W P + +KS+C +++T+FPFD Q C ++FGSW YD  Q+DII        +   ++D  D+  + EW            E  N T  K  +      Y       I K  P+            FY L L+IPCI LS LT++VF+LP     K+ L  ++ V+  V LL++ E  PS+ K  PLIG Y    MI +TLS  L    IN + R       +   VR+I +  L +LL +R H+         DR  TR  E G +R
Sbjct:   29 QDYQRWV---RPVEHLNDTVKVKFGLAITQLVDVDEKNQLMTTNVWLKQEWVDVKLRWDPNEFAGITSIRVPSDSIWIPDIVLYD-NADGRFEGSVTKAVVRYDGTINWTPPANYKSSCTIDVTFFPFDLQNCSMKFGSWTYDGSQVDII--------LEDYHVDKRDFFDNGEW------------EIVNATGNKGNRTDGCCFYPYITYSFIIKRLPL------------FYTLFLIIPCIGLSFLTILVFYLPSNEGEKISLCTSVLVSLTVFLLVIEEIIPSSSKVIPLIGEYLVFTMIFVTLSIVLTVFAINIHNRSSATHNAMAPWVRKIFLHKLPKLLCMRSHV---------DRYFTRKDETGKVR 357

[Close]

BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Xenopus
Match: rpap1 (RNA polymerase II associated protein 1 [Source:Xenbase;Acc:XB-GENE-976775])

HSP 1 Score: 190.274 bits (482), Expect = 8.237e-53
Identity = 134/373 (35.92%), Postives = 187/373 (50.13%), Query Frame = 3

Query:  321 NYQRYGIFGRPVSHSKELMKVQYGLQLIQLLDLDENKQILRTNCWSMYRWHDKLLEWNPAEHGGITEIRVFPDQIWTPDIKLYNFXXXXXXXXXXXXXXXKHTGKTLWVPQSIFKSTCAVEITYFPFDTQICMLEFGSWAYDIHQLDIIWWVPDGTNVSMPYIDFNDYLQSNEWRTDGEVDFQLKLEGDNKTQIKSVKKYRTRVYVSSNGQPINKHYPXXXXXXXXXXNSSFYVLILVIPCILLSSLTLVVFWLPPESPAKMMLGMNIXXXXXXXXXXXXESTPSAVKKFPLIGAYFCLNMIMITLSTFLAALVINYYFRGDFA-GPLPILVRRIIVEGLGRLLLVRQHIPLPDETNLMDRVLTRA-ENGCIR 1433
            +YQR+    RPV H  + +KV++GL + QL+D+DE  Q++ TN W    W D  L W+P E  GIT IRV  D IW PDI LY+       E  V + VV++ G   W P + +KS+C +++T+FPFD Q C ++FGSW YD  Q+DII        +   ++D  D+  + EW            E  N T  K  +      Y       I K  P+            FY L L+IPCI LS LT++VF+LP     K+ L  ++ V+  V LL++ E  PS+ K  PLIG Y    MI +TLS  L    IN + R       +   VR+I +  L +LL +R H+         DR  TR  E G +R
Sbjct:    8 DYQRWV---RPVEHLNDTVKVKFGLAITQLVDVDEKNQLMTTNVWLKQEWVDVKLRWDPNEFAGITSIRVPSDSIWIPDIVLYD-NADGRFEGSVTKAVVRYDGTINWTPPANYKSSCTIDVTFFPFDLQNCSMKFGSWTYDGSQVDII--------LEDYHVDKRDFFDNGEW------------EIVNATGNKGNRTDGCCFYPYITYSFIIKRLPL------------FYTLFLIIPCIGLSFLTILVFYLPSNEGEKISLCTSVLVSLTVFLLVIEEIIPSSSKVIPLIGEYLVFTMIFVTLSIVLTVFAINIHNRSSATHNAMAPWVRKIFLHKLPKLLCMRSHV---------DRYFTRKDETGKVR 335

[Close]

BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Mouse
Match: Chrna2 (cholinergic receptor, nicotinic, alpha polypeptide 2 (neuronal) [Source:MGI Symbol;Acc:MGI:87886])

HSP 1 Score: 192.586 bits (488), Expect = 6.487e-53
Identity = 127/383 (33.16%), Postives = 198/383 (51.70%), Query Frame = 3

Query:  222 WMIFLIFGLFLCRGTDTVNWPSVEKNLTVSILNNYQRYGIFGRPVSHSKELMKVQYGLQLIQLLDLDENKQILRTNCWSMYRWHDKLLEWNPAEHGGITEIRVFPDQIWTPDIKLYNFXXXXXXXXXXXXXXXKHTGKTLWVPQSIFKSTCAVEITYFPFDTQICMLEFGSWAYDIHQLDIIWWVPDGTNVSMPYIDFNDYLQSNEWRTDGEVDFQLKLEGDNKTQIKSVKKYRTRVYVSSNGQPINKHYPXXXXXXXXXXNSSFYVLILVIPCILLSSLTLVVFWLPPESPAKMMLGMNIXXXXXXXXXXXXESTPSAVKKFPLIGAYFCLNMIMITLSTFLAALVINYYFRGDFAGPLPILVRRIIVEGLGRLLLVRQHIP 1370
            W + L+  +   +G+ T      E  L   +   Y R+    RPV ++ +++ V++GL + QL+D+DE  Q++ TN W    W+D  L W+PAE G IT +RV  + IW PDI LYN AD       + +  +  TG   WVP +I+KS+C++++T+FPFD Q C ++FGSW YD  ++D+              +D  DY +S EW               N T   + KKY     +          YP + Y   +RR   FY + L+IPC+L+S LT++VF+LP E   K+ L +++ ++  V LLL+ E  PS     PLIG Y    MI +TLS  +   V+N + R      +P  VR  ++  + R L++ + +P
Sbjct:   17 WCLLLMPAVLAQQGSHT----HAEDRLFKHLFGGYNRWA---RPVPNTSDVVIVRFGLSIAQLIDVDEKNQMMTTNVWLKQEWNDYKLRWDPAEFGNITSLRVPSEMIWIPDIVLYNNADGEFAVTHMTKAHLFFTGTVHWVPPAIYKSSCSIDVTFFPFDQQNCKMKFGSWTYDKAKIDL--------EQMERTVDLKDYWESGEWAI------------INATGTYNSKKYDCCAEI----------YPDVTYYFVIRRLPLFYTINLIIPCLLISCLTVLVFYLPSECGEKITLCISVLLSLTVFLLLITEIIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHHRSPSTHNMPNWVRVALLGRVPRWLMMNRPLP 362

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Query:  222 WMIFLIFGLFLCRGTDTVNWPSVEKNLTVSILNNYQRYGIFGRPVSHSKELMKVQYGLQLIQLLDLDENKQILRTNCWSMYRWHDKLLEWNPAEHGGITEIRVFPDQIWTPDIKLYNFXXXXXXXXXXXXXXXKHTGKTLWVPQSIFKSTCAVEITYFPFDTQICMLEFGSWAYDIHQLDIIWWVPDGTNVSMPYIDFNDYLQSNEWRTDGEVDFQLKLEGDNKTQIKSVKKYRTRVYVSSNGQPINKHYPXXXXXXXXXXNSSFYVLILVIPCILLSSLTLVVFWLPPESPAKMMLGMNIXXXXXXXXXXXXESTPSAVKKFPLIGAYFCLNMIMITLSTFLAALVINYYFRGDFAGPLPILVRRIIVEGLGRLLLVRQHIP 1370
            W + L+  +   +G+ T      E  L   +   Y R+    RPV ++ +++ V++GL + QL+D+DE  Q++ TN W    W+D  L W+PAE G IT +RV  + IW PDI LYN AD       + +  +  TG   WVP +I+KS+C++++T+FPFD Q C ++FGSW YD  ++D+              +D  DY +S EW               N T   + KKY     +          YP + Y   +RR   FY + L+IPC+L+S LT++VF+LP E   K+ L +++ ++  V LLL+ E  PS     PLIG Y    MI +TLS  +   V+N + R      +P  VR  ++  + R L++ + +P
Sbjct:   17 WCLLLMPAVLAQQGSHT----HAEDRLFKHLFGGYNRWA---RPVPNTSDVVIVRFGLSIAQLIDVDEKNQMMTTNVWLKQEWNDYKLRWDPAEFGNITSLRVPSEMIWIPDIVLYNNADGEFAVTHMTKAHLFFTGTVHWVPPAIYKSSCSIDVTFFPFDQQNCKMKFGSWTYDKAKIDL--------EQMERTVDLKDYWESGEWAI------------INATGTYNSKKYDCCAEI----------YPDVTYYFVIRRLPLFYTINLIIPCLLISCLTVLVFYLPSECGEKITLCISVLLSLTVFLLLITEIIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHHRSPSTHNMPNWVRVALLGRVPRWLMMNRPLP 362

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BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Mouse
Match: Chrna6 (cholinergic receptor, nicotinic, alpha polypeptide 6 [Source:MGI Symbol;Acc:MGI:106213])

HSP 1 Score: 191.815 bits (486), Expect = 7.926e-53
Identity = 134/521 (25.72%), Postives = 241/521 (46.26%), Query Frame = 3

Query:  324 YQRYGIFGRPVSHSKELMKVQYGLQLIQLLDLDENKQILRTNCWSMYRWHDKLLEWNPAEHGGITEIRVFPDQIWTPDIKLYNFXXXXXXXXXXXXXXXKHTGKTLWVPQSIFKSTCAVEITYFPFDTQICMLEFGSWAYDIHQLDIIWWVPDGTNVSMPYIDFNDYLQSNEWRTDGEVDFQLKLEGDNKTQIKSVKKYRTRVYVSSNGQPINKHYPXXXXXXXXXXNSSFYVLILVIPCILLSSLTLVVFWLPPESPAKMMLGMNIXXXXXXXXXXXXESTPSAVKKFPLIGAYFCLNMIMITLSTFLAALVINYYFRGDFAGPLPILVRRIIVEGLGRLLLVRQHIPLPDETNLMDRVLTRAENGCIRKCRLKIPNLDSNKFLTSPVQTAEPPLQQDLSPRNTNPFINNDELAESNVWQTNVRPLRSYSSVYRNTPQASMTARPNPITDTEDSPLLMRCNTTLENDVRELKRYIRMFVNRQKENNRKNLISMEWRTLALVLDRLFFVLYISSILIAVIA 1886
            +  Y  F RPV +  + + V + L + QL ++DE  QI+ TN W  + W D  L W+P E+ GI  +RV  D IW PDI LYN A    +     + ++K+ G   W P +IFKS+C ++IT+FPFD Q C L+FGSW YD  ++D++        +    +D ND+ +++EW       ++  ++ +   +I +   Y   +          +  P+            FY + L+IPC+ +S LT++VF+LP +   K+ L +++ ++  V LL++ E+ PS     PL+G Y    MI +TLS  +   V+N ++R      +P  V+ I ++    +L++R+  PL D+T          E G ++                 P    + P +   + R  +  +      + +   +++ P +  SS       A   A                 N+   +DV ++   ++      K +N  N +  +W+ +A+V+DR+F  ++I   +   + 
Sbjct:   42 FAHYNRFIRPVENVSDPVTVHFELAITQLANVDEVNQIMETNLWLRHIWKDYRLRWDPTEYDGIETLRVPADNIWKPDIVLYNNAVGDFQVEGKTKALLKYDGVITWTPPAIFKSSCPMDITFFPFDHQNCSLKFGSWTYDKAEIDLL--------IIGSKVDMNDFWENSEWEIVDASGYKHDIKYNCCEEIYTDITYSFYI----------RRLPM------------FYTINLIIPCLFISFLTVLVFYLPSDCGEKVTLCISVLLSLTVFLLVITETIPSTSLVIPLVGEYLLFTMIFVTLSIVVTVFVLNIHYRTPATHTMPKWVKTIFLQAFPSILMMRK--PL-DKTK---------EAGGVK----------------DPKSHTKRPAKVKFTHRGESKLLKECHHCQKS---SDIAPGKRRSS----QQPARWVAE----------------NSEHSSDVEDVIESVQFIAENMKSHNETNEVEDDWKYMAMVVDRVFLWVFIIVCVFGTVG 481

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BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Mouse
Match: Chrna4 (cholinergic receptor, nicotinic, alpha polypeptide 4 [Source:MGI Symbol;Acc:MGI:87888])

HSP 1 Score: 194.512 bits (493), Expect = 1.071e-52
Identity = 118/347 (34.01%), Postives = 191/347 (55.04%), Query Frame = 3

Query:  324 YQRYGIFGRPVSHSKELMKVQYGLQLIQLLDLDENKQILRTNCWSMYRWHDKLLEWNPAEHGGITEIRVFPDQIWTPDIKLYNFXXXXXXXXXXXXXXXKHTGKTLWVPQSIFKSTCAVEITYFPFDTQICMLEFGSWAYDIHQLDIIWWVPDGTNVSM-PYIDFNDYLQSNEWRTDGEVDFQLKLEGDNKTQIKSVKKYRTRVYVSSNGQPINKHYPXXXXXXXXXXNSSFYVLILVIPCILLSSLTLVVFWLPPESPAKMMLGMNIXXXXXXXXXXXXESTPSAVKKFPLIGAYFCLNMIMITLSTFLAALVINYYFRGDFAGPLPILVRRIIVEGLGRLLLVRQ 1361
            +  Y  + RPV++  +++ V++GL + QL+D+DE  Q++ TN W    WHD  L W+P ++  +T IR+  + IW PDI LYN AD       + +  + + G+  W P +I+KS+C++++T+FPFD Q C ++FGSW YD  ++D+         VSM   +D  D+ +S EW                   + +V  Y TR Y     +   + YP + Y   +RR   FY + L+IPC+L+S LT++VF+LP E   K+ L +++ ++  V LLL+ E  PS     PLIG Y    MI +TLS  +   V+N + R      +P  VRR+ ++ + RLL +++
Sbjct:   47 FSGYNKWSRPVANISDVVLVRFGLSIAQLIDVDEKNQMMTTNVWVKQEWHDYKLRWDPGDYENVTSIRIPSELIWRPDIVLYNNADGDFAVTHLTKAHLFYDGRVQWTPPAIYKSSCSIDVTFFPFDQQNCTMKFGSWTYDKAKIDL---------VSMHSRVDQLDFWESGEW-----------------VIVDAVGTYNTRKY-----ECCAEIYPDITYAFIIRRLPLFYTINLIIPCLLISCLTVLVFYLPSECGEKVTLCISVLLSLTVFLLLITEIIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHHRSPRTHTMPAWVRRVFLDIVPRLLFMKR 362

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BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Mouse
Match: Chrna4 (cholinergic receptor, nicotinic, alpha polypeptide 4 [Source:MGI Symbol;Acc:MGI:87888])

HSP 1 Score: 194.512 bits (493), Expect = 1.472e-52
Identity = 118/347 (34.01%), Postives = 191/347 (55.04%), Query Frame = 3

Query:  324 YQRYGIFGRPVSHSKELMKVQYGLQLIQLLDLDENKQILRTNCWSMYRWHDKLLEWNPAEHGGITEIRVFPDQIWTPDIKLYNFXXXXXXXXXXXXXXXKHTGKTLWVPQSIFKSTCAVEITYFPFDTQICMLEFGSWAYDIHQLDIIWWVPDGTNVSM-PYIDFNDYLQSNEWRTDGEVDFQLKLEGDNKTQIKSVKKYRTRVYVSSNGQPINKHYPXXXXXXXXXXNSSFYVLILVIPCILLSSLTLVVFWLPPESPAKMMLGMNIXXXXXXXXXXXXESTPSAVKKFPLIGAYFCLNMIMITLSTFLAALVINYYFRGDFAGPLPILVRRIIVEGLGRLLLVRQ 1361
            +  Y  + RPV++  +++ V++GL + QL+D+DE  Q++ TN W    WHD  L W+P ++  +T IR+  + IW PDI LYN AD       + +  + + G+  W P +I+KS+C++++T+FPFD Q C ++FGSW YD  ++D+         VSM   +D  D+ +S EW                   + +V  Y TR Y     +   + YP + Y   +RR   FY + L+IPC+L+S LT++VF+LP E   K+ L +++ ++  V LLL+ E  PS     PLIG Y    MI +TLS  +   V+N + R      +P  VRR+ ++ + RLL +++
Sbjct:   47 FSGYNKWSRPVANISDVVLVRFGLSIAQLIDVDEKNQMMTTNVWVKQEWHDYKLRWDPGDYENVTSIRIPSELIWRPDIVLYNNADGDFAVTHLTKAHLFYDGRVQWTPPAIYKSSCSIDVTFFPFDQQNCTMKFGSWTYDKAKIDL---------VSMHSRVDQLDFWESGEW-----------------VIVDAVGTYNTRKY-----ECCAEIYPDITYAFIIRRLPLFYTINLIIPCLLISCLTVLVFYLPSECGEKVTLCISVLLSLTVFLLLITEIIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHHRSPRTHTMPAWVRRVFLDIVPRLLFMKR 362

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BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. UniProt/SwissProt
Match: sp|P23414|ACH1_SCHGR (Acetylcholine receptor subunit alpha-L1 OS=Schistocerca gregaria OX=7010 PE=2 SV=1)

HSP 1 Score: 205.682 bits (522), Expect = 2.333e-56
Identity = 128/357 (35.85%), Postives = 197/357 (55.18%), Query Frame = 3

Query:  294 KNLTVSILNNYQRYGIFGRPVSHSKELMKVQYGLQLIQLLDLDENKQILRTNCWSMYRWHDKLLEWNPAEHGGITEIRVFPDQIWTPDIKLYNFXXXXXXXXXXXXXXXKHTGKTLWVPQSIFKSTCAVEITYFPFDTQICMLEFGSWAYDIHQLDI--IWWVPDGTNVSMPYIDFNDYLQSNEWRTDGEVDFQLKLEGDNKTQIKSVKKYRTRVYVSSNGQPINKHYPXXXXXXXXXXNSSFYVLILVIPCILLSSLTLVVFWLPPESPAKMMLGMNIXXXXXXXXXXXXESTPSAVKKFPLIGAYFCLNMIMITLSTFLAALVINYYFRGDFAGPLPILVRRIIVEGLGRLLLVR 1358
            K L   +L+NY R     RPVS++ + + V+ GL+L QL+DL+   QIL TN W  + W D    W+PAE+GG+TE+ V  + IW PDI LYN AD       + + V+ HTGK +W P +IFKS+C +++ YFPFD Q C ++FGSW YD  Q+D+  I    D   V +  ID  +Y  S EW                   I  V   R   Y     +P    YP + + + LRR + FY + L++PC+ +S L+++VF+LP +S  K+ L ++I ++  +  LL++E  PS     PL+G Y    M+++ LS  +  +V+N ++R      +   VR++ +  L +LLL+R
Sbjct:   28 KRLYDDLLSNYNR---LIRPVSNNTDTVLVKLGLRLSQLIDLNLKDQILTTNVWLEHEWQDHKFRWDPAEYGGVTELYVPSEHIWLPDIVLYNNADGEYVVTTMTKAVLHHTGKVVWTPPAIFKSSCEIDVRYFPFDQQTCFMKFGSWTYDGDQIDLKHINQKYDDNKVKVG-IDLREYYPSVEW------------------DILGVPAERHEKYYPCCAEP----YPDIFFNITLRRKTLFYTVNLIVPCVGISYLSVLVFYLPADSGEKIALCISILLSQTMFFLLISEIIPSTSLALPLLGKYLLFTMVLVGLSVVITIMVLNVHYRKPSTHKMAPWVRKVFIRRLPKLLLMR 358

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BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. UniProt/SwissProt
Match: sp|P17644|ACH2_DROME (Acetylcholine receptor subunit alpha-like 2 OS=Drosophila melanogaster OX=7227 GN=nAChRalpha2 PE=2 SV=1)

HSP 1 Score: 202.601 bits (514), Expect = 4.590e-55
Identity = 161/566 (28.45%), Postives = 270/566 (47.70%), Query Frame = 3

Query:  267 DTVNWPSVEKNLTVSILNNYQRYGIFGRPVSHSKELMKVQYGLQLIQLLDLDENKQILRTNCWSMYRWHDKLLEWNPAEHGGITEIRVFPDQIWTPDIKLYNFXXXXXXXXXXXXXXXKHTGKTLWVPQSIFKSTCAVEITYFPFDTQICMLEFGSWAYDIHQLDI--IWWVPDGTNVSMPYIDFNDYLQSNEWRTDGEVDFQLKLEGDNKTQIKSVKKYRTRVYVSSNGQPINKHYPXXXXXXXXXXNSSFYVLILVIPCILLSSLTLVVFWLPPESPAKMMLGMNIXXXXXXXXXXXXESTPSAVKKFPLIGAYFCLNMIMITLSTFLAALVINYYFRGDFAGPLPILVRRIIVEGLGRLLLVRQHIPLPDETNLMDRVLTRAENGCIRKCRLKIPNLDSNKFLTSPVQT----AEPPLQQDLSPRNTNPFINNDELAESNVWQTNVRPLRSYSSVYRNTPQASMTARPNPITDTEDS----PLLMRCNTTLENDVRELKRYIRMFVNRQKENNRKNLISMEWRTLALVLDRLFFVLYISSILIAVIACIPSSTEPVIPAQYRD 1934
            +TV      K L   +L+NY R     RPVS++ + + V+ GL+L QL+DL+   QIL TN W  + W D   +W+P+E+GG+TE+ V  + IW PDI LYN AD       + + ++ +TGK +W P +IFKS+C +++ YFPFD Q C ++FGSW YD  Q+D+  I    D  N     ID  +Y  S EW                   I  V   R   Y     +P    YP + + + LRR + FY + L+IPC+ +S L+++VF+LP +S  K+ L ++I ++  +  LL++E  PS     PL+G Y    M+++ LS  +  +++N ++R      +   +R   ++ L +LLL+R    +P +  L D    +   G      LK       + L   +Q     + P   + +  R      N   +  +    TN       +     +  +     P+ ++  +DS        +    LE  +  +  +I+  + RQ E N ++    +W  +A+V+DRLF  L++ + L+     +  +     P+ Y D
Sbjct:   37 ETVQANPDAKRLYDDLLSNYNR---LIRPVSNNTDTVLVKLGLRLSQLIDLNLKDQILTTNVWLEHEWQDHKFKWDPSEYGGVTELYVPSEHIWLPDIVLYNNADGEYVVTTMTKAILHYTGKVVWTPPAIFKSSCEIDVRYFPFDQQTCFMKFGSWTYDGDQIDLKHISQKNDKDNKVEIGIDLREYYPSVEW------------------DILGVPAERHEKYYPCCAEP----YPDIFFNITLRRKTLFYTVNLIIPCVGISYLSVLVFYLPADSGEKIALCISILLSQTMFFLLISEIIPSTSLALPLLGKYLLFTMLLVGLSVVITIIILNIHYRKPSTHKMRPWIRSFFIKRLPKLLLMR----VPKDL-LRDLAANKINYG------LKFSKTKFGQALMDEMQMNSGGSSPDSLRRMQGRVGAGGCNGMHVTTA----TNRFSGLVGALGGGLSTLSGYNGLPSVLSGLDDSLSDVAARKKYPFELEKAIHNV-MFIQHHMQRQDEFNAED---QDWGFVAMVMDRLFLWLFMIASLVGTFVILGEA-----PSLYDD 553

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BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. UniProt/SwissProt
Match: sp|P43679|ACHB3_CHICK (Neuronal acetylcholine receptor subunit beta-3 OS=Gallus gallus OX=9031 GN=CHRNB3 PE=2 SV=2)

HSP 1 Score: 197.978 bits (502), Expect = 1.469e-54
Identity = 132/382 (34.55%), Postives = 205/382 (53.66%), Query Frame = 3

Query:  225 MIFLIFGLFLCRGTDTVNWPSVEKNLTVSILNNYQRYGIFGRPVSHSKELMKVQYGLQLIQLLDLDENKQILRTNCWSMYRWHDKLLEWNPAEHGGITEIRVFPDQIWTPDIKLYNFXXXXXXXXXXXXXXXKHTGKTLWVPQSIFKSTCAVEITYFPFDTQICMLEFGSWAYDIHQLDIIWWVPDGTNVSMPYIDFNDYLQSNEWRTDGEVDFQLKLEGDNKTQIKSVKKYRTRVYVSSNGQPINKHYPXXXXXXXXXXNSSFYVLILVIPCILLSSLTLVVFWLPPESPAKMMLGMNIXXXXXXXXXXXXESTPSAVKKFPLIGAYFCLNMIMITLSTFLAALVINYYFRGDFA-GPLPILVRRIIVEGLGRLLLVRQHI 1367
            M+ L+  +     +D     SV +N    + + +Q Y  + RPV +S + +KV +GL++ QL+D+DE  Q++ TN W    W D  L WNP E+GGIT IRV  + +W PDI L+  AD R   + + + +VK+ G   W+P + +KS+C +E+T+FPFD Q C ++FGSW YD   +D+I        +    +D  D+  + EW    E+     ++G+ K  + S                    YP + Y   LRR   FY L L+IPC+ LS LT++VF+LP +   K+ L  ++ V+  V LL++ E  PS+ K  PLIG Y    MI +TLS  +   VIN + R      P+   V+R+ ++ L RLL ++ H+
Sbjct:    1 MLCLMLCVLCWSRSDVAALGSVVENEDALLRHLFQGYQKWVRPVENSNDTIKVLFGLKISQLVDVDEKNQLMTTNVWLKQEWMDHKLSWNPEEYGGITAIRVPSESLWLPDIVLFENADGRFEGSLMTKAIVKYNGVVQWMPPASYKSSCTMELTFFPFDRQNCSMKFGSWTYDGSMVDLI--------LVDENVDTKDFFDNGEW----EILNAKGMKGNRKDGLYS--------------------YPFVTYSFVLRRLPLFYTLFLIIPCLGLSFLTVLVFYLPSDEGEKLSLSTSVLVSLTVFLLVIEEIIPSSSKVIPLIGEYLLFIMIFVTLSIIVTVFVINVHHRSSATYHPMAPWVKRLFLQKLPRLLCMKGHV 350

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BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. UniProt/SwissProt
Match: sp|P09481|ACHA3_CHICK (Neuronal acetylcholine receptor subunit alpha-3 OS=Gallus gallus OX=9031 GN=CHRNA3 PE=1 SV=2)

HSP 1 Score: 198.749 bits (504), Expect = 2.409e-54
Identity = 143/524 (27.29%), Postives = 252/524 (48.09%), Query Frame = 3

Query:  291 EKNLTVSILNNYQRYGIFGRPVSHSKELMKVQYGLQLIQLLDLDENKQILRTNCWSMYRWHDKLLEWNPAEHGGITEIRVFPDQIWTPDIKLYNFXXXXXXXXXXXXXXXKHTGKTLWVPQSIFKSTCAVEITYFPFDTQICMLEFGSWAYDIHQLDIIWWVPDGTNVSMPYIDFNDYLQSNEWRTDGEVDFQLKLEGDNKTQIKSVKKYRTRVYVSSNGQPINKHYPXXXXXXXXXXNSSFYVLILVIPCILLSSLTLVVFWLPPESPAKMMLGMNIXXXXXXXXXXXXESTPSAVKKFPLIGAYFCLNMIMITLSTFLAALVINYYFRGDFAGPLPILVRRIIVEGLGRLLLVRQHIPLPDETNLMD-RVLTRAENGCIRKCRLKIPNLDSNKFLTSPVQTAEPPLQQDLSPRNTNPFINNDELAESNVWQTNVRPLRSYSSVYRNTPQASMTARPNPITDTEDSPLLMRCNTTLENDVRELKRYIRMFVNRQKENNRKNLISMEWRTLALVLDRLFFVLYI 1859
            E  L  ++  NY +   F RPV ++ + + +Q+ + + QL+ +DE  QI+ TN W  + W+D  L WNP ++GG   IRV   QIW PDI LYN A    +     + ++K+TG   W+P +IFKS+C +++TYFPFD Q C ++FGSW+YD  ++D+   V  G+ +++      DY +S EW       ++  ++ +   +I +   Y   +          +  P+            FY + ++IPC+L+S LT++VF+LP +   K+ L +++ ++  V LL++ E+ PS     PLIG Y    MI +TLS  +   V+N ++R      +P+ VR I +  L R++ + +  P  DE N    +    +E            NL  N F +S ++  +            + F+  D       +Q        +S    N  ++S +   +P+             + L  ++R+    ++      K  N    I  +W+ +A+V+DR+F  ++I
Sbjct:   26 EHRLYAALFKNYNQ---FVRPVKNASDPVIIQFEVSMSQLVKVDEVNQIMETNLWLKHIWNDYKLRWNPVDYGGAEFIRVPSGQIWKPDIVLYNNAVGDFQVDDKTKALLKYTGDVTWIPPAIFKSSCKIDVTYFPFDYQNCTMKFGSWSYDKAKIDL---VLIGSTMNL-----KDYWESGEWAIIKAPGYKHDIKYNCCEEIYTDITYSLYI----------RRLPL------------FYTINMIIPCLLISFLTVLVFYLPSDCGEKVTLCISVLLSLTVFLLVITETIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHYRTPKTHTMPVWVRTIFLNLLPRIMFMTR--PTSDEENNQKPKPFYTSE----------FSNL--NCFNSSEIKCCK------------DGFVCQDMACSCCQYQR-----MKFSDFSGNLTRSSSSESVDPLFSF----------SVLSPEMRDAIESVKYIAENMKMQNEAKEIQDDWKYVAMVIDRIFLWVFI 475

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BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. UniProt/SwissProt
Match: sp|Q5IS77|ACHA4_PANTR (Neuronal acetylcholine receptor subunit alpha-4 OS=Pan troglodytes OX=9598 GN=CHRNA4 PE=2 SV=1)

HSP 1 Score: 199.134 bits (505), Expect = 1.976e-53
Identity = 121/358 (33.80%), Postives = 198/358 (55.31%), Query Frame = 3

Query:  288 VEKNLTVSILNNYQRYGIFGRPVSHSKELMKVQYGLQLIQLLDLDENKQILRTNCWSMYRWHDKLLEWNPAEHGGITEIRVFPDQIWTPDIKLYNFXXXXXXXXXXXXXXXKHTGKTLWVPQSIFKSTCAVEITYFPFDTQICMLEFGSWAYDIHQLDIIWWVPDGTNVSMPYIDFNDYLQSNEWRTDGEVDFQLKLEGDNKTQIKSVKKYRTRVYVSSNGQPINKHYPXXXXXXXXXXNSSFYVLILVIPCILLSSLTLVVFWLPPESPAKMMLGMNIXXXXXXXXXXXXESTPSAVKKFPLIGAYFCLNMIMITLSTFLAALVINYYFRGDFAGPLPILVRRIIVEGLGRLLLVRQ 1361
             E+ L   + + Y ++    RPV++  +++ V++GL + QL+D+DE  Q++ TN W    WHD  L W+PA++  +T IR+  + IW PDI LYN AD       + +  + H G+  W P +I+KS+C++++T+FPFD Q C ++FGSW YD  ++D++       N+    +D  D+ +S EW                   + +V  Y TR Y     +   + YP + Y   +RR   FY + L+IPC+L+S LT++VF+LP E   K+ L +++ ++  V LLL+ E  PS     PLIG Y    MI +TLS  +   V+N + R      +P  VRR+ ++ + RLLL+++
Sbjct:   36 AEERLLKKLFSGYNKWS---RPVANISDVVLVRFGLSIAQLIDVDEKNQMMTTNVWVKQEWHDYKLRWDPADYENVTSIRIPSELIWRPDIVLYNNADGDFAVTHLTKAHLFHDGRVQWTPPAIYKSSCSIDVTFFPFDQQNCTMKFGSWTYDKAKIDLV-------NMHS-RVDQLDFWESGEW-----------------VIVDAVGTYNTRKY-----ECCAEIYPDITYAFVIRRLPLFYTINLIIPCLLISCLTVLVFYLPSECGEKITLCISVLLSLTVFLLLITEIIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHHRSPRTHTMPTWVRRVFLDIVPRLLLMKR 360

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BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. TrEMBL
Match: A0A4Z2D9U5 (Neuronal acetylcholine receptor subunit alpha-2 OS=Schistosoma japonicum OX=6182 GN=EWB00_003131 PE=3 SV=1)

HSP 1 Score: 558.525 bits (1438), Expect = 0.000e+0
Identity = 327/577 (56.67%), Postives = 404/577 (70.02%), Query Frame = 3

Query:  285 SVEKNLTVSILNNYQRYGIFGRPVSHSKELMKVQYGLQLIQLLDLDENKQILRTNCWSMYRWHDKLLEWNPAEHGGITEIRVFPDQIWTPDIKLYNFXXXXXXXXXXXXXXXKHTGKTLWVPQSIFKSTCAVEITYFPFDTQICMLEFGSWAYDIHQLDIIWWVPDGTNVS-MPYIDFNDYLQSNEWRTDGEVDFQLKLEGDNKTQIKSVKKYRTRVYVSSNGQPINKHYPXXXXXXXXXXNSSFYVLILVIPCILLSSLTLVVFWLPPESPAKMMLGMNIXXXXXXXXXXXXESTPSAVKKFPLIGAYFCLNMIMITLSTFLAALVINYYFRGDFAGPLPILVRRIIVEGLGRLLLVRQHIPLPDET-----------------NLMDRVLTRAENGCIRKCRLKIPNLDSNKFLTSPVQTAEPPL-QQDLSPRNTNPFINNDELA--------ESNVWQTNVRPLRSYSSVYRNTPQASMTARPNPITDTEDSPLLMRCNTTLENDVRELKRYIRMFVNRQKENNRKNLISMEWRTLALVLDRLFFVLYISSILIAVIACIPSSTEPVIPAQYRD 1934
            SVEKNL   +LN Y+++G+ GRPV+ SK  + V+YGLQL Q+LDLDENKQILRTNCWSMY+W D LL WN +++G I  +R+FP QIWTPDIKLYNFAD RLRE R ARVVV   G+ LWVPQ++FKSTC VEITYFPFDTQICMLEFGSW YD  Q+DI+WW+PD   +  MPY+DF+DY+ SNEWRTDGE + ++    +   QI+SVKK+R R     N + I + YPVLRYLVRLRRN SFYV +LVIPC+LLSSLTLVVFWLPPESPAKMMLGMNIFVAFFVLLLLLAESTPSAVK FPLIG YFCLNMIMITLSTFLA LVI+ YFRGD  G +P ++RRII+EG+GRL LVRQ IPLPD                   N+    LT+ E  C +  R++  +     F    +  + P   ++ L    +N F  ND  +        +S    T +  +           + S    P+   +  +SP     +TTLE+DVRELK+Y++M VNRQKE  RK+L++MEWRTLA+VLDRLFF +YI++I+IAV+  +P STEP +P  +RD
Sbjct:   36 SVEKNLIRMLLNRYEQFGVIGRPVNDSKIKVDVRYGLQLFQILDLDENKQILRTNCWSMYKWTDSLLRWNESDYGNIRTVRIFPSQIWTPDIKLYNFADERLREHRDARVVVSSNGEMLWVPQALFKSTCEVEITYFPFDTQICMLEFGSWTYDKTQMDILWWIPDSPPIEEMPYLDFSDYVPSNEWRTDGEKEREVH-HVNRTIQIRSVKKHRRRSQTVGN-EVITREYPVLRYLVRLRRNPSFYVFMLVIPCVLLSSLTLVVFWLPPESPAKMMLGMNIFVAFFVLLLLLAESTPSAVKNFPLIGVYFCLNMIMITLSTFLATLVIHLYFRGDRNGSVPYILRRIIIEGIGRLTLVRQRIPLPDMKKPIRPAVIKPPKRIQIPNMSPDKLTKNEETCCQNERVR--HFAGGTFQQCNMVGSRPLYGEEHLGGAASNWFAPNDRRSVSRYPSQFDSYAIATPINDILPIPGYLGQAARKSSVNGPDNTVNQNESPSATSPSTTLEHDVRELKKYVKMLVNRQKETARKSLVAMEWRTLAIVLDRLFFFIYITTIMIAVVVSVPRSTEPEVPPAFRD 608

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BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. TrEMBL
Match: A0A5K3FFX3 (Uncharacterized protein OS=Mesocestoides corti OX=53468 PE=4 SV=1)

HSP 1 Score: 553.518 bits (1425), Expect = 0.000e+0
Identity = 316/569 (55.54%), Postives = 402/569 (70.65%), Query Frame = 3

Query:  285 SVEKNLTVSILNNYQRYGIFGRPVSHSKELMKVQYGLQLIQLLDLDENKQILRTNCWSMYRWHDKLLEWNPAEHGGITEIRVFPDQIWTPDIKLYNFXXXXXXXXXXXXXXXKHTGKTLWVPQSIFKSTCAVEITYFPFDTQICMLEFGSWAYDIHQLDIIWWVPDGTNVSMPYIDFNDYLQSNEWRTDGEVDFQLKLEGDNKTQIKSVKKYRTRVYVSSNGQPIN-KHYPXXXXXXXXXXNSSFYVLILVIPCILLSSLTLVVFWLPPESPAKMMLGMNIXXXXXXXXXXXXESTPSAVKKFPLIGAYFCLNMIMITLSTFLAALVINYYFRGDFAGPLPILVRRIIVEGLGRLLLVRQHIPLPDETNLMDRVLTRAENGCIRKC---RLKIPNLDSNKFLT------SPVQTAEPPLQQDLSP----RNTNPFINNDELAESNVWQT----------NVRPLRSYSSV------YRNTPQASMTARPNPITDTEDSPLLMRCNTTLENDVRELKRYIRMFVNRQKENNRKNLISMEWRTLALVLDRLFFVLYISSILIAVIACIPSS 1901
            SVEK L   ++  Y+R+G+ GRPVS +K ++KV YGLQLIQ+LDLDENKQIL TNCWSMYRW+D LL+WNPAEHG +TE+R+FP QIWTPDI+L+NFAD R+RE R+ARVVV   G  LWVPQ++FKS C VEITYFPFDTQIC LEFGSW YD  Q+++ WWV   + +  PY+DF DY+ SNEWRTDGE +  + +  +   QIK+ K+Y+ R    ++G  +  K Y VL Y VRL RN SFY+ ILV+PCILLSSLT+VVFWLPPESPAKMMLGMNIFV FF+LLL+LA  TP+AVK+FPLIGAYFCLNMIMITLSTFLA LVI++++RG   GPLP ++ RII+EG+GRL+LVRQ IPLP+E   +  V+TR   G   +    +L++P L S+KFL       SP +TA   L   +      RN  P   +      +   T          +V  L   S        + ++   ++ A P    D+EDSP+++  +T+LE DVRELKRY+RMFVNRQKE +RKN I+MEWRT+ALVLDRLFF +YI++I I V+  +P S
Sbjct:   36 SVEKALIRMLIERYRRFGVIGRPVSDNKIVLKVNYGLQLIQILDLDENKQILHTNCWSMYRWNDTLLQWNPAEHGNVTEVRIFPSQIWTPDIQLFNFADERIREHRIARVVVHSDGSILWVPQALFKSACQVEITYFPFDTQICNLEFGSWTYDRTQMELDWWVTTSSPIPNPYVDFVDYVPSNEWRTDGETERDI-VHTNRTKQIKAYKRYQERN--QTDGDVVTTKQYTVLCYRVRLHRNPSFYIFILVVPCILLSSLTIVVFWLPPESPAKMMLGMNIFVTFFLLLLVLAGQTPNAVKEFPLIGAYFCLNMIMITLSTFLATLVIHFHYRGARNGPLPAIIHRIIIEGMGRLMLVRQSIPLPEEKKTLSHVVTRTAAGLGGRHARRKLRVPELHSSKFLGDDDLDDSPQRTATGALPNCIDGGSGWRNHVPMSGSRSGFGPSCAATTCPGCMGLADSVGDLSRRSGGVPGTLDWHSSTVDAVRAPPAGNADSEDSPIMLSSSTSLERDVRELKRYVRMFVNRQKEGSRKNAIAMEWRTMALVLDRLFFFVYIATIGITVVTSLPRS 601

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BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. TrEMBL
Match: A0A5K3FBT6 (Uncharacterized protein OS=Mesocestoides corti OX=53468 PE=4 SV=1)

HSP 1 Score: 553.132 bits (1424), Expect = 0.000e+0
Identity = 316/569 (55.54%), Postives = 402/569 (70.65%), Query Frame = 3

Query:  285 SVEKNLTVSILNNYQRYGIFGRPVSHSKELMKVQYGLQLIQLLDLDENKQILRTNCWSMYRWHDKLLEWNPAEHGGITEIRVFPDQIWTPDIKLYNFXXXXXXXXXXXXXXXKHTGKTLWVPQSIFKSTCAVEITYFPFDTQICMLEFGSWAYDIHQLDIIWWVPDGTNVSMPYIDFNDYLQSNEWRTDGEVDFQLKLEGDNKTQIKSVKKYRTRVYVSSNGQPIN-KHYPXXXXXXXXXXNSSFYVLILVIPCILLSSLTLVVFWLPPESPAKMMLGMNIXXXXXXXXXXXXESTPSAVKKFPLIGAYFCLNMIMITLSTFLAALVINYYFRGDFAGPLPILVRRIIVEGLGRLLLVRQHIPLPDETNLMDRVLTRAENGCIRKC---RLKIPNLDSNKFLT------SPVQTAEPPLQQDLSP----RNTNPFINNDELAESNVWQT----------NVRPLRSYSSV------YRNTPQASMTARPNPITDTEDSPLLMRCNTTLENDVRELKRYIRMFVNRQKENNRKNLISMEWRTLALVLDRLFFVLYISSILIAVIACIPSS 1901
            SVEK L   ++  Y+R+G+ GRPVS +K ++KV YGLQLIQ+LDLDENKQIL TNCWSMYRW+D LL+WNPAEHG +TE+R+FP QIWTPDI+L+NFAD R+RE R+ARVVV   G  LWVPQ++FKS C VEITYFPFDTQIC LEFGSW YD  Q+++ WWV   + +  PY+DF DY+ SNEWRTDGE +  + +  +   QIK+ K+Y+ R    ++G  +  K Y VL Y VRL RN SFY+ ILV+PCILLSSLT+VVFWLPPESPAKMMLGMNIFV FF+LLL+LA  TP+AVK+FPLIGAYFCLNMIMITLSTFLA LVI++++RG   GPLP ++ RII+EG+GRL+LVRQ IPLP+E   +  V+TR   G   +    +L++P L S+KFL       SP +TA   L   +      RN  P   +      +   T          +V  L   S        + ++   ++ A P    D+EDSP+++  +T+LE DVRELKRY+RMFVNRQKE +RKN I+MEWRT+ALVLDRLFF +YI++I I V+  +P S
Sbjct:   11 SVEKALIRMLIERYRRFGVIGRPVSDNKIVLKVNYGLQLIQILDLDENKQILHTNCWSMYRWNDTLLQWNPAEHGNVTEVRIFPSQIWTPDIQLFNFADERIREHRIARVVVHSDGSILWVPQALFKSACQVEITYFPFDTQICNLEFGSWTYDRTQMELDWWVTTSSPIPNPYVDFVDYVPSNEWRTDGETERDI-VHTNRTKQIKAYKRYQERN--QTDGDVVTTKQYTVLCYRVRLHRNPSFYIFILVVPCILLSSLTIVVFWLPPESPAKMMLGMNIFVTFFLLLLVLAGQTPNAVKEFPLIGAYFCLNMIMITLSTFLATLVIHFHYRGARNGPLPAIIHRIIIEGMGRLMLVRQSIPLPEEKKTLSHVVTRTAAGLGGRHARRKLRVPELHSSKFLGDDDLDDSPQRTATGALPNCIDGGSGWRNHVPMSGSRSGFGPSCAATTCPGCMGLADSVGDLSRRSGGVPGTLDWHSSTVDAVRAPPAGNADSEDSPIMLSSSTSLERDVRELKRYVRMFVNRQKEGSRKNAIAMEWRTMALVLDRLFFFVYIATIGITVVTSLPRS 576

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BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. TrEMBL
Match: A0A3Q0KEK5 (Putative nachr subunit OS=Schistosoma mansoni OX=6183 PE=3 SV=1)

HSP 1 Score: 552.747 bits (1423), Expect = 0.000e+0
Identity = 325/577 (56.33%), Postives = 406/577 (70.36%), Query Frame = 3

Query:  285 SVEKNLTVSILNNYQRYGIFGRPVSHSKELMKVQYGLQLIQLLDLDENKQILRTNCWSMYRWHDKLLEWNPAEHGGITEIRVFPDQIWTPDIKLYNFXXXXXXXXXXXXXXXKHTGKTLWVPQSIFKSTCAVEITYFPFDTQICMLEFGSWAYDIHQLDIIWWVPDGTNVS-MPYIDFNDYLQSNEWRTDGEVDFQLKLEGDNKTQIKSVKKYRTRVYVSSNGQPINKHYPXXXXXXXXXXNSSFYVLILVIPCILLSSLTLVVFWLPPESPAKMMLGMNIXXXXXXXXXXXXESTPSAVKKFPLIGAYFCLNMIMITLSTFLAALVINYYFRGDFAGPLPILVRRIIVEGLGRLLLVRQHIPLPDETNLMDRVLTRAENGCIRKCRLKIPNLDSNKFLTSPVQTAE--------PPLQQDLSPRNTNPFINNDEL--AESNVWQTNV-RPLRSYSSVYRNTPQA---------------SMTARPNPITDTEDSPLLMRCNTTLENDVRELKRYIRMFVNRQKENNRKNLISMEWRTLALVLDRLFFVLYISSILIAVIACIPSSTEPVIPAQYRD 1934
            SVEKNL   +LN Y+++G+ GRPV+ SK  + V+YGLQL Q+LDLDENKQILRTNCWSMY+W D LL WN +++G I  +R+FP QIWTPDIKLYNFAD RLRE R ARVVV   G+ LWVPQ++FKSTC VEITYFPFDTQICMLEFGSW YD  Q+DI+WW+PD   +  MPY+DF+DY+ SNEWRTDGE + ++    +   QI+SVKK+R R     N + I + YPVLRYL+RLRRN SFYV +LVIPC+LLSSLTLVVFWLPPESPAKMMLGMNIFVAFFVLLLLLAESTPSAVK FPLIG YFCLNMIMITLSTFLA LVI+ YFRGD  G +P ++RRII+EG+GRL LVRQ IPLP+    +   + +         +++IP +  NK   +     E            Q  +   ++     ++L  A SN +  N  R +  Y + + + P A               S    P+   +  +SP ++   TTLE DVRELK+Y++M VNRQKE  RK+L++MEWRTLA+VLDRLFF +YI++I+IAV+  +P STEP +P  +RD
Sbjct:   36 SVEKNLIRMLLNRYEQFGVIGRPVNDSKIKVDVRYGLQLFQILDLDENKQILRTNCWSMYKWTDSLLRWNESDYGDIKTVRIFPSQIWTPDIKLYNFADERLREHRDARVVVSSNGEMLWVPQALFKSTCEVEITYFPFDTQICMLEFGSWTYDKTQMDILWWIPDSPPIEEMPYLDFSDYVPSNEWRTDGEKEREVH-HVNRTIQIRSVKKHRRRSQTVGN-EVITREYPVLRYLIRLRRNPSFYVFMLVIPCVLLSSLTLVVFWLPPESPAKMMLGMNIFVAFFVLLLLLAESTPSAVKNFPLIGVYFCLNMIMITLSTFLATLVIHLYFRGDRNGSVPYVLRRIIIEGIGRLTLVRQRIPLPEMKKPIRPSIIKPHK------KIQIPGISPNKIPANEETYCENERIRHFGGGAFQQCNMVGSHFLYGEEQLGGAASNWFAPNEHRSVPRYPNQFDSYPTATQINNISQIPGSAGKSSENEPDNTANHNESPSVLNSTTTLERDVRELKKYVKMLVNRQKETARKSLVAMEWRTLAIVLDRLFFFIYITTIVIAVVVSVPRSTEPEVPPAFRD 604

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BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. TrEMBL
Match: A0A068Y539 (Neuronal acetylcholine receptor subunit alpha 4 OS=Echinococcus multilocularis OX=6211 GN=EmuJ_000462600 PE=3 SV=1)

HSP 1 Score: 549.666 bits (1415), Expect = 0.000e+0
Identity = 318/586 (54.27%), Postives = 402/586 (68.60%), Query Frame = 3

Query:  285 SVEKNLTVSILNNYQRYGIFGRPVSHSKELMKVQYGLQLIQLLDLDENKQILRTNCWSMYRWHDKLLEWNPAEHGGITEIRVFPDQIWTPDIKLYNFXXXXXXXXXXXXXXXKHTGKTLWVPQSIFKSTCAVEITYFPFDTQICMLEFGSWAYDIHQLDIIWWVPDGTNVSMPYIDFNDYLQSNEWRTDGEVDFQLKLEGDNKTQIKSVKKYRTRVYVSSNGQPIN-KHYPXXXXXXXXXXNSSFYVLILVIPCILLSSLTLVVFWLPPESPAKMMLGMNIXXXXXXXXXXXXESTPSAVKKFPLIGAYFCLNMIMITLSTFLAALVINYYFRGDFAGPLPILVRRIIVEGLGRLLLVRQHIPLPDETNLMDRVLTRAENGCI-----RKCRLKIPNLDSNKFL------------TSPVQTAEPPLQQDLSPRNTNPFINNDELAESNVWQTNVRPLRSYSSVYRNTP-------------------------QASMT----ARPNPITDTEDSPLLMRCNTTLENDVRELKRYIRMFVNRQKENNRKNLISMEWRTLALVLDRLFFVLYISSILIAVIACIPSS 1901
            SVEK L   ++  Y+RYG+ GRPVS SK ++KV+YGLQLIQ+LDLDENKQIL TNCWSMY+W+D LL+WNPAEHG +TE+R+FP Q+WTPDI+L+NFAD R++E R+ARVVV   G  LWVPQ++FKSTC VEITYFPFDTQIC LEFGSW YD  QL++ WWV   + + M Y+DF DY+ SNEWRTDGE +  + +  +   QIK+ K+Y+ R    ++G  +  K Y VL Y VRL RN SFY+ ILV+PCILLSSLT+VVFWLPPESPAKMMLGMNIFV FF+LLL+LA  TP+AVK+FPLIGAYFCLNMIMITLSTFLA LVI++++RG   GPLP ++ RII+EG+GRL++VRQ IPLP+E   +  V+TR   G +     R+ ++++P L SNKFL            T+     E P      P  T   I+     ++ +     R   S S  +   P                         QAS T      P    D+EDSP+++  +T+LE DVRELKRY+RMFVNRQKE  RKN I+MEWRT+ALVLDRLFF +YI++I I V+  +P S
Sbjct:   36 SVEKALIRMLIERYRRYGVIGRPVSDSKTVLKVRYGLQLIQILDLDENKQILHTNCWSMYKWNDSLLQWNPAEHGNVTEVRIFPSQVWTPDIQLFNFADERIQEHRIARVVVHSDGSILWVPQALFKSTCQVEITYFPFDTQICTLEFGSWTYDRTQLELDWWVSSSSPIPMAYVDFVDYVPSNEWRTDGEEERDI-VHTNRTKQIKAYKRYQERN--QTDGDVVTTKQYTVLCYRVRLHRNPSFYIFILVVPCILLSSLTIVVFWLPPESPAKMMLGMNIFVTFFLLLLVLAGQTPNAVKEFPLIGAYFCLNMIMITLSTFLATLVIHFHYRGARNGPLPAILHRIIIEGMGRLMMVRQSIPLPEEKKTLSHVVTRTGAGGLGGRSSRRRKVRVPELPSNKFLEDDDDDDDHINNTNTTTNNESPRCPPFGP--TTNCIDGGGGWKNQMSGAGNRAAYSVSGAHATCPGCIGLGDSVGDLSRRSGGTSGPLDWQASATEPLRGAPAANLDSEDSPIMLSSSTSLERDVRELKRYVRMFVNRQKEGARKNAIAMEWRTMALVLDRLFFFVYIATIGITVVTSLPRS 616

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BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Cavefish
Match: chrna2a (cholinergic receptor, nicotinic, alpha 2a (neuronal) [Source:ZFIN;Acc:ZDB-GENE-040108-2])

HSP 1 Score: 207.223 bits (526), Expect = 3.576e-58
Identity = 147/534 (27.53%), Postives = 261/534 (48.88%), Query Frame = 3

Query:  324 YQRYGIFGRPVSHSKELMKVQYGLQLIQLLDLDENKQILRTNCWSMYRWHDKLLEWNPAEHGGITEIRVFPDQIWTPDIKLYNFXXXXXXXXXXXXXXXKHTGKTLWVPQSIFKSTCAVEITYFPFDTQICMLEFGSWAYDIHQLDIIWWVPDGTNVSMPYIDFNDYLQSNEWRTDGEVDFQLKLEGDNKTQIKSVKKYRTRVYVSSNGQPINKHYPXXXXXXXXXXNSSFYVLILVIPCILLSSLTLVVFWLPPESPAKMMLGMNIXXXXXXXXXXXXESTPSAVKKFPLIGAYFCLNMIMITLSTFLAALVINYYFRGDFAGPLPILVRRIIVEGLGRLLLVRQHIPLPDETNLMDRVLTRAENGCIRKCRLKIPNLDSNKFLTSPVQTAEPPLQQDLSPRNTNPFINNDELAESNVWQTNVRPLRSYSSVYRNTP-------------QASMTARPNPITDTEDSPLLMRCNTTLENDVRELKRYIRMFVNRQKENNRKNLISMEWRTLALVLDRLFFVLYISSILIAVIA 1886
            +  Y  + RPV +  +++ V++GL + QL+D+DE  Q++ TN W    W+D  L W P+++  +T IRV  + IW PDI LYN AD       + +  + HTGK LWVP +I+KS+C++++T+FPFD Q C ++FGSW YD  ++D+              +D  DY +S EW                   + +V  Y T+ Y        ++ YP + Y   +RR   FY + L+IPC+L+S LT++VF+LP +   K+ L +++ ++  V LLL+ E  PS     PLIG Y    MI +TLS  +   V+N + R      +P  V+ I +  + R L +++  P PD+T    R+L  A+       + ++PN  SN      + T+     Q  +  +   + +++  A   +  +   P  +   +  + P             + ++ +RP  +++ E  P L        + V +    ++   +  +  +    +  +W+ +A+V+DR+F  ++I   L+  + 
Sbjct:   31 FSGYNKWSRPVRNITDVVIVKFGLSIAQLIDVDEKNQMMTTNVWLKQEWNDYKLRWKPSDYDNVTSIRVPSELIWVPDIVLYNNADGEFAVTHMTKAHLFHTGKVLWVPPAIYKSSCSIDVTFFPFDQQNCKMKFGSWTYDKAKIDL--------ERIENTVDLKDYWESGEWAI-----------------VNAVGTYNTKKY-----DCCHEIYPDITYFFIIRRLPLFYTINLIIPCLLISCLTVLVFYLPSDCGEKITLCISVLLSLTVFLLLITEIIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHHRSPSTHKMPGWVQSIFLGFIPRWLFMKR--PAPDQTR--RRLLLNAQE------KKQVPNQTSN------LSTSRSSWLQQETDVDIGRYQDSNGRAGMGIPASFSFPTATSLHLSESEPLLQSLYDLQQLGHRINLNSRPVNLSNLEPEPRL-----PFSHSVMQALEGVQYIADHLRAEDADFSVKEDWKYVAMVIDRIFLWMFIIVCLLGTVG 513

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BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Cavefish
Match: chrna4b (neuronal acetylcholine receptor subunit alpha-2 [Source:NCBI gene;Acc:103025036])

HSP 1 Score: 198.364 bits (503), Expect = 1.379e-54
Identity = 119/346 (34.39%), Postives = 188/346 (54.34%), Query Frame = 3

Query:  324 YQRYGIFGRPVSHSKELMKVQYGLQLIQLLDLDENKQILRTNCWSMYRWHDKLLEWNPAEHGGITEIRVFPDQIWTPDIKLYNFXXXXXXXXXXXXXXXKHTGKTLWVPQSIFKSTCAVEITYFPFDTQICMLEFGSWAYDIHQLDIIWWVPDGTNVSMPYIDFNDYLQSNEWRTDGEVDFQLKLEGDNKTQIKSVKKYRTRVYVSSNGQPINKHYPXXXXXXXXXXNSSFYVLILVIPCILLSSLTLVVFWLPPESPAKMMLGMNIXXXXXXXXXXXXESTPSAVKKFPLIGAYFCLNMIMITLSTFLAALVINYYFRGDFAGPLPILVRRIIVEGLGRLLLVRQ 1361
            + RY    RPV++  +++ V +GL + QL+D+DE  Q++ TN W    W D  L WNP E+  +T IR+  + IW PDI LYN AD       + +  + H G+  W P +I+KS+C++++T+FPFD Q C ++FGSW YD  ++D++    D        +D  DY +S EW                   I +V KY T+ Y     +   + YP + Y   +RR   FY + L+IPC+L+S LT++VF+LP E   K+ L +++ ++  V LLL+ E  PS     PLIG Y    MI +TLS  +   V+N + R      +P  VR++ ++ + R L +++
Sbjct:   36 FSRYNKLSRPVANISDVVLVHFGLSIAQLIDVDEKNQMMTTNVWVKQEWSDYKLRWNPEEYENVTSIRIPSELIWRPDIVLYNNADGDFAVTHLTKAQLFHDGRIKWKPPAIYKSSCSIDVTFFPFDQQNCKMKFGSWTYDRAKIDLVSMDDD--------VDQMDYWESGEW-----------------VIINAVGKYNTKKY-----ECCTEIYPDITYSFIIRRLPLFYTINLIIPCLLISCLTVLVFYLPSECAEKITLCISVLLSLTVFLLLITEIIPSTSLVIPLIGEYLLFTMIFVTLSIIITVFVLNVHHRSSRTHRMPHWVRQLFLQLVPRYLFMKR 351

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BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Cavefish
Match: chrna4b (neuronal acetylcholine receptor subunit alpha-2 [Source:NCBI gene;Acc:103025036])

HSP 1 Score: 197.978 bits (502), Expect = 4.640e-54
Identity = 119/346 (34.39%), Postives = 188/346 (54.34%), Query Frame = 3

Query:  324 YQRYGIFGRPVSHSKELMKVQYGLQLIQLLDLDENKQILRTNCWSMYRWHDKLLEWNPAEHGGITEIRVFPDQIWTPDIKLYNFXXXXXXXXXXXXXXXKHTGKTLWVPQSIFKSTCAVEITYFPFDTQICMLEFGSWAYDIHQLDIIWWVPDGTNVSMPYIDFNDYLQSNEWRTDGEVDFQLKLEGDNKTQIKSVKKYRTRVYVSSNGQPINKHYPXXXXXXXXXXNSSFYVLILVIPCILLSSLTLVVFWLPPESPAKMMLGMNIXXXXXXXXXXXXESTPSAVKKFPLIGAYFCLNMIMITLSTFLAALVINYYFRGDFAGPLPILVRRIIVEGLGRLLLVRQ 1361
            + RY    RPV++  +++ V +GL + QL+D+DE  Q++ TN W    W D  L WNP E+  +T IR+  + IW PDI LYN AD       + +  + H G+  W P +I+KS+C++++T+FPFD Q C ++FGSW YD  ++D++    D        +D  DY +S EW                   I +V KY T+ Y     +   + YP + Y   +RR   FY + L+IPC+L+S LT++VF+LP E   K+ L +++ ++  V LLL+ E  PS     PLIG Y    MI +TLS  +   V+N + R      +P  VR++ ++ + R L +++
Sbjct:   39 FSRYNKLSRPVANISDVVLVHFGLSIAQLIDVDEKNQMMTTNVWVKQEWSDYKLRWNPEEYENVTSIRIPSELIWRPDIVLYNNADGDFAVTHLTKAQLFHDGRIKWKPPAIYKSSCSIDVTFFPFDQQNCKMKFGSWTYDRAKIDLVSMDDD--------VDQMDYWESGEW-----------------VIINAVGKYNTKKY-----ECCTEIYPDITYSFIIRRLPLFYTINLIIPCLLISCLTVLVFYLPSECAEKITLCISVLLSLTVFLLLITEIIPSTSLVIPLIGEYLLFTMIFVTLSIIITVFVLNVHHRSSRTHRMPHWVRQLFLQLVPRYLFMKR 354

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BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Cavefish
Match: chrna2b (neuronal acetylcholine receptor subunit alpha-2 [Source:NCBI gene;Acc:103031133])

HSP 1 Score: 193.356 bits (490), Expect = 1.634e-53
Identity = 122/370 (32.97%), Postives = 195/370 (52.70%), Query Frame = 3

Query:  333 YGIFGRPVSHSKELMKVQYGLQLIQLLDLDENKQILRTNCWSMYRWHDKLLEWNPAEHGGITEIRVFPDQIWTPDIKLYNFXXXXXXXXXXXXXXXKHTGKTLWVPQSIFKSTCAVEITYFPFDTQICMLEFGSWAYDIHQLDIIWWVPDGTNVSMPYIDFNDYLQSNEWRTDGEVDFQLKLEGDNKTQIKSVKKYRTRVYVSSNGQPINKHYPXXXXXXXXXXNSSFYVLILVIPCILLSSLTLVVFWLPPESPAKMMLGMNIXXXXXXXXXXXXESTPSAVKKFPLIGAYFCLNMIMITLSTFLAALVINYYFRGDFAGPLPILVRRIIVEGLGRLLLVRQHIPLPDETNLMDRVLTRAENGCIRKCR 1442
            Y  + RPV ++ +++ V++GL + QL+D+DE  Q++ TN W    W+D  L W P+++  +T IRV  + IW PDI LYN AD       + +  + +TGK  WVP +I+KS+C++++T+FPFD Q C ++FGSW YD  ++D+              +D  DY +S EW                   I +V  Y T+ Y        ++ YP + Y   +RR   FY + L+IPC+L+S LT++VF+LP +   K+ L +++ ++  V LLL+ E  PS     PLIG Y    MI +TLS  +   V+N + R      +P  V  + ++ + R L +R+  P PD       +L +    C+   R
Sbjct:   64 YNKWSRPVPNTSDVVIVKFGLSIAQLIDVDEKNQMMTTNVWLKQEWNDYKLRWKPSDYDNVTSIRVPSELIWVPDIVLYNNADGEFAVTHMTKAHLFYTGKVRWVPPAIYKSSCSIDVTFFPFDQQNCKMKFGSWTYDKAKIDL--------ERLETMVDLKDYWESGEWAI-----------------INAVGTYNTKKY-----DCCHEIYPDITYFFIIRRLPLFYTINLIIPCLLISCLTVLVFYLPSDCGEKITLCISVLLSLTVFLLLITEIIPSTSLVIPLIGEYLLFTMIFVTLSIIITVFVLNVHHRSPGTHEMPHWVHAVFLDLIPRWLFMRR--PAPDSRRRQRLLLLQQGMACLPNSR 401

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BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Cavefish
Match: chrnb3b (neuronal acetylcholine receptor subunit non-alpha-2 [Source:NCBI gene;Acc:103031723])

HSP 1 Score: 192.586 bits (488), Expect = 1.806e-53
Identity = 129/379 (34.04%), Postives = 197/379 (51.98%), Query Frame = 3

Query:  234 LIFGLFLCRGTDTVNWPSVEKNLTVSILNNYQRYGIFGRPVSHSKELMKVQYGLQLIQLLDLDENKQILRTNCWSMYRWHDKLLEWNPAEHGGITEIRVFPDQIWTPDIKLYNFXXXXXXXXXXXXXXXKHTGKTLWVPQSIFKSTCAVEITYFPFDTQICMLEFGSWAYDIHQLDIIWWVPDGTNVSMPY-IDFNDYLQSNEWRTDGEVDFQLKLEGDNKTQIKSVKKYRTRVYVSSNGQPINKHYPXXXXXXXXXXNSSFYVLILVIPCILLSSLTLVVFWLPPESPAKMMLGMNIXXXXXXXXXXXXESTPSAVKKFPLIGAYFCLNMIMITLSTFLAALVINYYFRGDFA-GPLPILVRRIIVEGLGRLLLVRQH 1364
            L++  +LC  T    + S+ +     + + +Q Y  + RP  HS   ++V++GL++ QL+D+DE  Q++ TN W    W D  L WNP ++GGIT IRV  + IW PDI LY  AD R   + + + ++K+ G   W P + +KS C +++T+FPFD Q C ++FGSW YD   +D+         V M Y +D  D+  + EW            E  + T +K  ++  T  Y       I K  P+            FY L L+IPC+ LS LT++VF+LP +   K+ L  ++ V+  V LL++ E  PS+ K  PLIG Y    MI +TLS  +   VIN + R      P+   VR + ++ L  LL +R H
Sbjct:    5 LVWLYWLCSSTSGGEFVSLAEREDALLRDLFQGYQRWVRPTQHSNNTVRVRFGLKISQLVDVDEKNQLMTTNVWLWQEWIDYKLRWNPEDYGGITFIRVPSESIWLPDIVLYENADGRFEGSLMTKAIIKYNGAVTWTPPASYKSACTMDVTFFPFDRQNCSMKFGSWTYDGTMVDM---------VLMNYQVDRRDFFDNGEW------------EILSATGMKGNRRDGTLSYPFVTYSFILKRLPL------------FYTLFLIIPCLGLSFLTVLVFYLPSDEGEKVSLSTSVLVSLTVFLLVIEEIIPSSSKVIPLIGEYLLFIMIFVTLSIIVTVFVINVHHRSSATYHPMSPWVRSLFLQRLPHLLCMRGH 350

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BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Sea Lamprey
Match: chrnb3b (cholinergic receptor, nicotinic, beta 3b (neuronal) [Source:ZFIN;Acc:ZDB-GENE-030102-4])

HSP 1 Score: 195.282 bits (495), Expect = 3.777e-55
Identity = 130/371 (35.04%), Postives = 194/371 (52.29%), Query Frame = 3

Query:  288 VEKNLTVSILNNYQRYGIFGRPVSHSKELMKVQYGLQLIQLLDLDENKQILRTNCWSMYRWHDKLLEWNPAEHGGITEIRVFPDQIWTPDIKLYNFXXXXXXXXXXXXXXXKHTGKTLWVPQSIFKSTCAVEITYFPFDTQICMLEFGSWAYDIHQLDIIWWVPDGTNVSMPYIDFNDYLQSNEWRTDGEVDFQLKLEGDNKTQIKSVKKYRTRVYVSSNGQPINKHYPXXXXXXXXXXNSSFYVLILVIPCILLSSLTLVVFWLPPESPAKMMLGMNIXXXXXXXXXXXXESTPSAVKKFPLIGAYFCLNMIMITLSTFLAALVINYYFRGDFA-GPLPILVRRIIVEGLGRLLLVRQHI---PLPDETN 1388
            +E  L   +L +YQ++    RPV    + ++V++GL + QL+D+DE  Q++ TN W    W D  L WNP ++GGI  IRV    IW PDI LY+ AD R   + V + VVKH G+  W P +I+KS+C +++T+FPFD Q C ++FGSW YD   +D+I    D        +D  +Y  + EW            E  N T  K V+    R Y       + +  P+            FY L L++PC+ LS LT+ VF+LP +   K+ L  ++ V+  V LL++ E  PS+ K  PLIG Y    MI +TLS  +   VIN + R      P+   VR++ +  L ++L +R H+     PDE +
Sbjct:   34 LEDRLLKRMLKDYQKWV---RPVDKLSDTVEVKFGLGIAQLVDVDEKNQLMTTNVWLKQEWVDINLRWNPDDYGGINSIRVPASSIWLPDIVLYDNADGRFEGSIVTKAVVKHDGRIKWTPPAIYKSSCTIDVTFFPFDQQNCSMKFGSWTYDGTLVDLIMVNSD--------VDRGEYFDNGEW------------EFLNATGNKGVRLDGHRFYPYITYSFVIRRLPL------------FYTLFLIVPCLGLSFLTVFVFYLPSDEGEKISLCTSVLVSLTVFLLVIEEIIPSSSKVIPLIGEYLLFIMIFVTLSIIITVFVINVHHRSSSTHHPMAPWVRQLFLHKLPKILCMRSHVDRYTAPDEAD 369

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BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Sea Lamprey
Match: chrna3 (cholinergic receptor, nicotinic, alpha 3 [Source:ZFIN;Acc:ZDB-GENE-070822-1])

HSP 1 Score: 187.578 bits (475), Expect = 3.897e-52
Identity = 119/345 (34.49%), Postives = 192/345 (55.65%), Query Frame = 3

Query:  324 YQRYGIFGRPVSHSKELMKVQYGLQLIQLLDLDENKQILRTNCWSMYRWHDKLLEWNPAEHGGITEIRVFPDQIWTPDIKLYNFXXXXXXXXXXXXXXXKHTGKTLWVPQSIFKSTCAVEITYFPFDTQICMLEFGSWAYDIHQLDIIWWVPDGTNVSMPYIDFNDYLQSNEWRTDGEVDFQLKLEGDNKTQIKSVKKYRTRVYVSSNGQPINKHYPXXXXXXXXXXNSSFYVLILVIPCILLSSLTLVVFWLPPESPAKMMLGMNIXXXXXXXXXXXXESTPSAVKKFPLIGAYFCLNMIMITLSTFLAALVINYYFRGDFAGPLPILVRRIIVEGLGRLLLVR 1358
            +  Y  + RPV +  + + V++ + + QL+ LDE  QI+ TN W  + W+D   +WNP +  G+  IRV  D+IW PDI LYN AD   +     + ++K+ G  +W+P +IFKS+C +++TYFPFD Q C ++FGSW YD  ++D+   V  GT      I+  D+ +S EW                   I  V  Y+  +  +   Q     YP + Y V +RR   FY + L+IPC+L+S LT++VF+LP +   K+ L +++ ++  V LL++ E+ PS     PLIG Y    MI +TLS  +   V+N ++R   A  +P  VRR+ ++ L +++L++
Sbjct:   16 FTSYNPYIRPVFNVSDPVIVRFEVSMSQLVKLDEVNQIMATNLWLKHTWNDYKFQWNPGQFEGMEYIRVPSDKIWKPDIVLYNNADGDFQVEGKTKALLKYDGTVMWMPPAIFKSSCKIDVTYFPFDYQNCTMKFGSWTYDKAKIDL---VLVGTK-----INLKDFWESGEW------------------VIIDVPGYKHDIKYNCCEQ----IYPDITYSVYVRRLPLFYTINLIIPCLLISFLTVLVFYLPSDCGEKVTLSISVLLSLTVFLLVITETIPSTSLVIPLIGEYLLFTMIFVTLSIVITIFVLNVHYRTPEAHTMPNWVRRVFLDTLPKIMLLQ 330

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BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Sea Lamprey
Match: chrna2a (cholinergic receptor, nicotinic, alpha 2a (neuronal) [Source:ZFIN;Acc:ZDB-GENE-040108-2])

HSP 1 Score: 182.956 bits (463), Expect = 7.566e-50
Identity = 118/345 (34.20%), Postives = 181/345 (52.46%), Query Frame = 3

Query:  381 VQYGLQLIQLLDLDENKQILRTNCWSMYRWHDKLLEWNPAEHGGITEIRVFPDQIWTPDIKLYNFXXXXXXXXXXXXXXXKHTGKTLWVPQSIFKSTCAVEITYFPFDTQICMLEFGSWAYDIHQLDIIWWVPDGTNVSMPYIDFNDYLQSNEWRTDGEVDFQLKLEGDNKTQIKSVKKYRTRVYVSSNGQPINKHYPXXXXXXXXXXNSSFYVLILVIPCILLSSLTLVVFWLPPESPAKMMLGMNIXXXXXXXXXXXXESTPSAVKKFPLIGAYFCLNMIMITLSTFLAALVINYYFRGDFAGPLPILVRRIIVEGLGRLLLVRQHIPLPDETNLMDRVLTRA 1415
            V+Y      L D DE  Q++ TN W    W D  L+W+PAE+  +T IRV  + IW PDI LYN AD       + +  + HTG+  W P +I+KS+C++++T+FPFD Q C ++FGSW YD  ++D++    D        +D  DY +S EW                   + +V  Y T+ Y         + YP + Y   +RR   FY + L+IPC+L+S LT++VF+LP +   K+ L +++ ++  V LLL+ E  PS     PLIG Y    MI +TLS  +   V+N + R      +P  VRR+ ++ + R L++R+  P    + L+   L RA
Sbjct:    1 VRYAEFRTHLCDCDEKNQMMTTNVWLKQEWDDYKLQWDPAEYENVTSIRVPSELIWVPDIVLYNNADGDFAVTHMTKAHLFHTGRVRWKPPAIYKSSCSIDVTFFPFDQQNCTMKFGSWTYDKAKIDLVSMARD--------VDLKDYWESGEWAI-----------------VHAVGTYNTKKYDCCP-----QVYPDITYSFIIRRLPLFYTINLIIPCLLISCLTVLVFYLPSDCGEKITLCISVLLSLTVFLLLITEIIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHHRSPATHAMPRWVRRVFLDRVPRWLVMRRPPPQQRRSELL---LARA 312

[Close]

BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Sea Lamprey
Match: chrnb1l (cholinergic receptor, nicotinic, beta 1 (muscle) like [Source:ZFIN;Acc:ZDB-GENE-060811-1])

HSP 1 Score: 139.043 bits (349), Expect = 2.976e-35
Identity = 108/401 (26.93%), Postives = 187/401 (46.63%), Query Frame = 3

Query:  327 QRYGIFGRPVSHSKELMKVQYGLQLIQLLDLDENKQILRTNCWSMYRWHDKLLEWNPAEHGGITEIRVFPDQIWTPDIKLYNFXXXXXXXXXXXXXXXKHTGKTLWVPQSIFKSTCAVEITYFPFDTQICMLEFGSWAYDIHQLDIIWWVPDG-TNVSMPYIDFNDYLQSNEWRTDGEVDFQLKLEGDNKTQIKSVKKYRTRVYVSSNGQPINKHYPXXXXXXXXXXNSSFYVLILVIPCILLSSLTLVVFWLPPESPAKMMLGMNIXXXXXXXXXXXXESTPSAVKKFPLIGAYFCLNMIMITLSTFLAALVINYYFRGDFAGPLPILVRRIIVEGLGRLLLVRQHIPLPDETNLMDRVLTRAENGCIRKCRLKIPNLDSNKFLTSPVQTAEPPLQQDLS 1526
            Q+Y    RP   +   + V+ G+ L QL+ L   +  +  +      W D  L W+P  +  +  IR+ P ++W PDI L N  D          V+V +TG   WVP SI++S+C +++ YFPFD Q C + F S+ YD  ++++ + + +    +    ID + +  S EW        Q+K +   K                 N  P +  Y  + + + + R   FY++ +V+PCIL++   +  F+LPP++  KM L +++ +A  V LLLLA+  P      P+I  Y    M+++T    L  +V+N +FR      +P  VRRI +  L + L + +  P  +E      VLT+ +  C  +  ++ P   +   L +       P   +L+
Sbjct:   35 QQYKTLVRPARTATGRVTVRLGMTLSQLISLPACQPDMTLSRLISLSWEDPRLSWDPRNYDNLRSIRLSPKRVWLPDIALSNNNDGNFDFGLYVHVLVDYTGAVRWVPPSIYRSSCGMQVKYFPFDWQNCSMVFQSFTYDTSEINLEYALDEHRQEIRHIIIDESTFPVSGEW--------QIKHKPSRK-----------------NTNPRDPLYQDITFYLIMERKPLFYIINIVVPCILITICAVFTFYLPPDAGEKMGLCISVLLALTVFLLLLADKVPETSLGVPVIVNYLMFTMVIVTACVILTVIVLNLHFRSPATHIMPYWVRRIFIHSLPKYLWIER--PKKEELAPKLPVLTQHQQHCHDEYFIRTPPSQALLLLPTSPSFGMTPFSSELA 408

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BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008550.1 (pep scaffold:Pmarinus_7.0:GL476601:34:38373:1 gene:ENSPMAG00000007717.1 transcript:ENSPMAT00000008550.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 137.502 bits (345), Expect = 3.889e-35
Identity = 126/486 (25.93%), Postives = 210/486 (43.21%), Query Frame = 3

Query:  501 EHGGITEIRVFPDQIWTPDIKLYNFXXXXXXXXXXXXXXXKHTGKTLWVPQSIFKSTCAVEITYFPFDTQICMLEFGSWAYDIHQLDIIWWVPDGTNVSMPYIDFNDYLQSNEWRTDGEVDFQLKLEGDNKTQIKSVKKYRTRVYVSSNGQPINKHYPXXXXXXXXXXNSSFYVLILVIPCILLSSLTLVVFWLPPESPAKMMLGMNIXXXXXXXXXXXXESTPSAVKKFPLIGAYFCLNMIMITLSTFLAALVINYYFRGDFAGPLPILVRRIIVEGLGRLLLVRQHIPLPDETNLMDRVLTRAENGCIRKCRLKIPNLDSNKF------------LTSPVQTAEPPLQQDLSPRNTNPFINNDELAESNVWQTNVRPLRSYSSVYRNTPQASMTARPNPITDTEDSPLLMRCNTTLENDVRELKRYIRMFVNRQKENNRKNLISMEWRTLALVLDR---LFFVLYISSILIAVIACIPSSTEPV 1913
            EH G+  +R  P QIWTPDI LYN AD R        V+V +TG    +P  + KSTC +++ +FPFD Q C L+FGSW +D        W+ D   + M   D + Y+ + EW                   +  +   R  +Y     +P    YP + Y + +RR + +Y L L++PC+L+S+L+L+VF LP +S  K+ LG+ + ++  V +LL+AE  P+     PLIG YF   MI++  S  +  +V+ Y+        +P  VR I+++     L ++Q  P  +   L  R+     N  +    L +  L ++               T+P    +PP              ++  +        +  P     +  R  P       P+P                + +++ ++   +R    R +  +    I+ EW+  A V+DR   L F L+     I+++   P  TE V
Sbjct:    2 EHPGVKNLRFQPHQIWTPDILLYNSADERFDATFHTNVLVNYTGNCQHLPPGMLKSTCYIDVRWFPFDVQKCDLKFGSWTHD-------GWLID---LQMLEADTSGYIANGEW------------------DLVEIPGKRNELYYDCCQEP----YPDVTYTLTIRRRTLYYGLNLLVPCVLISALSLLVFLLPADSGEKISLGITVLLSLTVFMLLVAEIMPATSDSVPLIGQYFASTMIIVAFSVVVTVIVLQYHHHNPDGKKMPQWVRVILLDWCAWFLRMKQ--PGRERHELARRLSILHRNSMV---SLDLALLSASGIHKKTNGHGVFIGFTNPDAPQQPPTS------------DSGVMCGRETCFNSTGPEEEPGNAQRGMP-------PDP-------------TAGMSSEITKILIELRYIAGRFRTQDENEAIASEWKFAAAVVDRLCLLAFTLFTIISTISILMSAPHFTEAV 418

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BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Nematostella
Match: EDO40862 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S622])

HSP 1 Score: 157.147 bits (396), Expect = 5.757e-43
Identity = 104/330 (31.52%), Postives = 176/330 (53.33%), Query Frame = 3

Query:  291 EKNLTVSILNNYQRYGIFGRPVSHSKELMKVQYGLQLIQLLDLDENKQILRTNCWSMYRWHDKLLEWNPAEHGGITEIRVFPDQIWTPDIKLYNFXXXX---XXXXXXXXXXXKHTGKTLWVPQSIFKSTCAVEITYFPFDTQICMLEFGSWAYDIHQLDIIWWVPDGTNVSMPYIDFNDYLQSNEWRTDGEVDFQLKLEGDNKTQIKSVKKYRTRVYVSSNGQPINKHYPXXXXXXXXXXNSSFYVLILVIPCILLSSLTLVVFWLPPESPAKMMLGMNIXXXXXXXXXXXXESTPSAVKKFPLIGAYFCLNMIMITLSTFLAALVINY 1271
            E+ L   +  NY +Y    RP   S     V +  +L Q++DL +N+Q+L+   ++  +W + +L W+P ++GG+ EI V P+++W PDI +YN A+     L       VV++  G  +W+  ++F +TC +++ YFPFD Q C+L+FGSW +D  +LD+     +G+N  +       Y +  EW                  Q+  V++ R   Y     +P    Y  + Y V +RR S FY L L++P +L++S+ LV F LPPES  +M LG+ + +A  V L  + ++ PS     PL+G ++ + M +I+LS     +++NY
Sbjct:    1 EQRLLRDLFQNYSKYA---RPSIESDAPAIVTFRAKLKQIVDLKDNEQLLKIILYNYMQWENPILTWDPRDYGGVQEINVAPEEVWVPDIVIYNSAEDVLGGLTHKYKTDVVIQANGTNIWLAPAMFLTTCQLDVRYFPFDEQKCLLKFGSWTHDSSRLDMRTQT-NGSNKDI-------YTEHGEW------------------QLVGVEQKRNVKYYDCCPKP----YVDISYTVHIRRKSLFYSLYLILPALLITSMVLVGFHLPPESGERMQLGITVLLAMIVFLQFVYQNLPSTSDSAPLLGKFYIVMMFLISLSLGCTCIILNY 297

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BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Nematostella
Match: EDO42054 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S2J7])

HSP 1 Score: 161.384 bits (407), Expect = 7.018e-43
Identity = 111/358 (31.01%), Postives = 179/358 (50.00%), Query Frame = 3

Query:  285 SVEKNLTVSILNNYQRYGIFGRPVSHSKELMKVQYGLQLIQLLDLDENKQILRTNCWSMYRWHDKLLEWNPAEHGGITEIRVFPDQIWTPDIKLYNFXXXXXX---XXXXXXXXXKHTGKTLWVPQSIFKSTCAVEITYFPFDTQICMLEFGSWAYDIHQLDIIWWVPDGTNVSMPYIDFNDYLQSNEWRTDGEVDFQLKLEGDNKTQIKSVKK--YRTRVYVSSNGQPINKHYPXXXXXXXXXXNSSFYVLILVIPCILLSSLTLVVFWLPPESPAKMMLGMNIXXXXXXXXXXXXESTPSAVKKFPLIGAYFCLNMIMITLSTFLAALVINYYFRGDFAGPLPILVRRIIVEGLGR 1343
            + EK L   +  NY       RPV +  + + V++ L LIQ++ ++   Q++  + W   +W + +L WN +++GGI  + + P ++W PDI LYN AD       E     V+    G   W   + F STC + + YFPFD+Q C L+FGSW Y  ++L +       T+ S    D + Y+ + EW     +    KL   N+      K   Y   +Y+    +P+                  +YV  L+IPC L+++LTL+ F+LPPES  K+ LG+ + +A  V LLL+AE+ PS     PL+G YF   M +  +S     L++N++ R     P+P  V+ II++ + R
Sbjct:   36 TAEKRLLADLFRNYDSEV---RPVFNDSDKVNVEFSLSLIQIIAVNSKSQLVTVSVWIRQKWTNPMLAWNTSKYGGIESVNLNPAKVWIPDIILYNNADETYAGGLEKYKTSVIASSDGTQEWNSPASFTSTCNINVRYFPFDSQNCSLKFGSWTYVGNRLTM-------TSTS-DSADLSKYVNNGEWHL---LAMPAKL---NRVFYNCCKYPFYDVTLYLEIQRKPL------------------YYVFNLIIPCALIATLTLIKFFLPPESGEKVGLGITVLLAMTVFLLLVAETLPSTSDHIPLLGQYFVATMFVTAISLVATCLILNFFHRNPSTSPMPHWVKVIILDYMAR 358

[Close]

BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Nematostella
Match: EDO42072 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S2P1])

HSP 1 Score: 157.918 bits (398), Expect = 8.252e-43
Identity = 113/362 (31.22%), Postives = 182/362 (50.28%), Query Frame = 3

Query:  291 EKNLTVSILNNYQRYGIFGRPVSHSKELMKVQYGLQLIQLLDLDENKQILRTNCWSMYRWHDKLLEWNPAEHGGITEIRVFPDQIWTPDIKLYNFXXX---XXXXXXXXXXXXKHTGKTLWVPQSIFKSTCAVEITYFPFDTQICMLEFGSWAYDIHQLDIIWWVPDGTNVSMPYIDFNDYLQSNEWRTDGEVDFQLKLEGDNKTQIKSVKKYRTRVYVSSNGQPINKHYPXXXXXXXXXXNSSFYVLILVIPCILLSSLTLVVFWLPPESPAKMMLGMNIXXXXXXXXXXXXESTPSAVKKFPLIGAYFCLNMIMITLSTFLAALVINY---YFRGDFAGPLPILVRRIIVEGLGRLLLVR 1358
            E  L + +  NY ++    RPV +    + V+ GL L Q+  L E  Q L+T+ W    W ++ L W+P E GGI+ I V  D++W PDI LYN AD            ++ +   G  +W+   +  S+C +++TYFPFD Q C L+FGSW YD  +LD++   P G        D + Y+ S +W                   + S    R  ++     Q     YP + + + +RR + FY + L++PC L++ LTL+ F+LPPES  ++ L +   +A  V +LL+A+  P   +  PLI  Y+ L M+ + L+  L A  ++Y   YF       +P LVR ++++ L +L  V+
Sbjct:    1 EARLHMFLFENYNKHL---RPVVNKSRALTVRLGLTLHQIASLVEKSQFLQTSLWVRQMWRNEYLTWDPKEFGGISTINVNVDKVWVPDIYLYNNADADGDGALNKFGTQIKLLFNGINIWLAPVLLTSSCKIDVTYFPFDEQHCSLKFGSWTYDGERLDLVSESPSG--------DVSKYVPSGQW------------------DLVSFTAKRNEIFYVCCPQ----SYPDVTFTLNIRRKTIFYYMNLIMPCFLITGLTLLSFFLPPESGERITLVITNLLAMTVFMLLVADWLPPTSEVVPLISMYYTLTMMEVGLA--LVATCLDYLKCYFMNPVTTEMPFLVRLLVLKWLAKLTRVK 327

[Close]

BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Nematostella
Match: EDO43642 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RY59])

HSP 1 Score: 155.221 bits (391), Expect = 2.718e-42
Identity = 106/312 (33.97%), Postives = 166/312 (53.21%), Query Frame = 3

Query:  324 YQRYGIFGRPVSHSKELMKVQYGLQLIQLLDLDENKQILRTNCWSMYRWHDKLLEWNPAEHGGITEIRVFPDQIWTPDIKLYNFX---XXXXXXXXXXXXXXKHTGKTLWVPQSIFKSTCAVEITYFPFDTQICMLEFGSWAYDIHQLDIIWWVPD-GTNVSMPYIDFNDYLQSNEWRTDGEVDFQLKLEGDNKTQIKSVKKYRTRVYVSSNGQPINKHYPXXXXXXXXXXNSSFYVLILVIPCILLSSLTLVVFWLPPESPAKMMLGMNIXXXXXXXXXXXXESTPSAVKKFPLIGAYFCLNMIMITLSTF 1247
            +  Y    RPV  +   + VQ G+ L Q++DLDE  QIL +N W   +W++ LL W+P   G +T I V P ++W PD+ LYN A        +    +V +K  G +LW+   IFKSTC  ++ YFPFD Q C+L+FGSW YD  +L+I   VP+ GT++ +     + ++ + EW   G                  V   R  ++     +P    YP + + +++RR + FY + ++IPC L++ LTL+ + LPPES  ++ L +   +A  V LLL++E  P   +  PLI  Y+   M M+T+  F
Sbjct:   10 FSNYNPSVRPVLKATSSVTVQLGVSLHQIIDLDERNQILISNLWVRQKWNNPLLTWDPTHFGNLTSINVDPLRVWRPDLILYNNADRDTDGALDRFYTKVQLKFDGSSLWLAPIIFKSTCKFDVAYFPFDEQSCILKFGSWTYDGLRLNI---VPENGTDIGI-----DKFVSNGEWELTG------------------VSANRNVMFYDCCVEP----YPDVTFTLKIRRLTLFYYMNMLIPCFLITGLTLLSYILPPESGERITLVVTSLLAQAVYLLLVSEIMPPVAEVVPLISIYYTTAMFMVTVFKF 291

[Close]

BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Nematostella
Match: EDO47969 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RKU6])

HSP 1 Score: 155.606 bits (392), Expect = 5.457e-42
Identity = 107/363 (29.48%), Postives = 180/363 (49.59%), Query Frame = 3

Query:  291 EKNLTVSILNNYQRYGIFGRPVSHSKELMKVQYGLQLIQLLDLDENKQILRTNCWSMYRWHDKLLEWNPAEHGGITEIRVFPDQIWTPDIKLYNFXXXXXX---XXXXXXXXXKHTGKTLWVPQSIFKSTCAVEITYFPFDTQICMLEFGSWAYDIHQLDIIWWVPDGTNVSMPYIDFNDYLQSNEWRTDGEVDFQLKLEGDNKTQIKSVKKYRTRVYVSSNGQPINKHYPXXXXXXXXXXNSSFYVLILVIPCILLSSLTLVVFWLPPESPAKMMLGMNIXXXXXXXXXXXXESTPSAVKKFPLIGAYFCLNMIMITLSTFLAALVINYYFRGD---FAGPLPILVRRIIVEGLGRLLLVRQ 1361
            E+ L   +L  Y R     RPV +S   +KV +G++++Q+++++   QI++TN W   RW ++LL W+P+ + G+  +R+    +W PDI LY+ AD       E     V++   G + W   + F+STC +++TYFPFD Q C ++FGSW Y I  +DI          S   +  + YL+S EW                   + S    R     +   +PI+     +   + +RR   FY   L+ PC+++ ++ L+ F+LPPES  ++ L + + +A  V L L+AE+ P   +  PL+G ++   M  I +S      V+N + R         +P  V  I++  L  +L VRQ
Sbjct:    1 EQRLISDLLQGYSRNA---RPVLNSNHTLKVFFGMEIVQIVNVENRNQIIQTNVWIRQRWDNELLTWHPSNYEGVQTVRLDAKSVWIPDIVLYDSADSVFSGGHEKYKTHVIISKDGSSAWFSPASFRSTCKIDVTYFPFDEQTCYMKFGSWTYTIADVDI--------QASPAPVKSSKYLKSAEW------------------DLMSATTSRKSQIYACCPEPISD----VTIAMTIRRKPLFYAFNLITPCMIMLTMILLGFFLPPESGERITLSITVLLAMAVFLQLVAETLPRNSESIPLLGRFYITIMTEIAVSLMATCWVLNIHHRNSGTTLVVEMPKWVDVIVLRWLAVVLCVRQ 330

[Close]

BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Medaka
Match: si:ch211-39a7.1 (neuronal acetylcholine receptor subunit alpha-7-like [Source:NCBI gene;Acc:101160921])

HSP 1 Score: 196.823 bits (499), Expect = 5.319e-54
Identity = 159/579 (27.46%), Postives = 266/579 (45.94%), Query Frame = 3

Query:  291 EKNLTVSILNNYQRYGIFGRPVSHSKELMKVQYGLQLIQLLDLDENKQILRTNCWSMYRWHDKLLEWNPAEHGGITEIRVFPDQIWTPDIKLYNFXXXXXXXXXXXXXXXKHTGKTLWVPQSIFKSTCAVEITYFPFDTQICMLEFGSWAYDIHQLDIIWWVPDGTNVSMPYIDFNDYLQSNEWRTDGEVDFQLKLEGDNKTQIKSVKKYRTRVYVSSNGQPINKHYPXXXXXXXXXXNSSFYVLILVIPCILLSSLTLVVFWLPPESPAKMMLGMNIXXXXXXXXXXXXESTPSAVKKFPLIGAYFCLNMIMITLSTFLAALVINYYFRGDFAGPLPILVRRIIVEGLGRLLLVRQHIPLPDETNLMDRVLTRAENGCIRKCRLKIPNLDSNKFLTSPVQTAEPPLQQDLSPRNTNP---------------------FINNDELAESNVWQTNVR------PLRSYSSVYRNTPQASMTARPNPIT--DTEDSP-----------LLMRCNTTLEND--VRELKRYIRMFVNRQKENNRKNLISMEWRTLALVLDRLFFVLYISSILIAVIACIPSS 1901
            +++L  S+L +Y R     RPV++    + V + L LIQ++D+DE  Q+L +N W    W+D  L+WN +E+ G+  +R   DQ+WTPDI LYN AD          V+V  +G   ++P  IF STC V++ +FPFD Q C L+FGSW YD   LD+           M   D + Y+ + EW   G                  V   R  ++     +P    Y  + +++ +RR + +Y L L+IPC+LLSS+TL+VF LP +S  K+ LG+ + ++  V +LL+AE  P+     PLIG YF   MI++ +S     +V+ Y+      G +P  V+ ++++ +   L +++    P E++  +R         +R+C        S      P  T  P   Q L+P  T P                     ++    + +S+V    V+      P R   S   + P    ++ P P +  DT   P            +  C+T    D  ++ +   +R   +R +E +    ++ +W+    V+DRL  V +    +I  I+ + S+
Sbjct:   25 QRSLLKSLLKDYNR---MERPVANDSHTLTVHFTLSLIQIMDVDEKNQVLTSNMWLQMTWYDHYLQWNQSEYPGVKNLRFTSDQVWTPDILLYNSADDDFDSTFKTNVLVNSSGYAKYLPPGIFMSTCNVDVRWFPFDIQKCNLKFGSWTYDGWLLDL----------QMIEADISSYMPNGEWDLIG------------------VPGSRNEIFYDCCKEP----YTDVTFVITIRRRTLYYALNLLIPCVLLSSMTLLVFVLPADSGEKISLGITVLLSLTVFMLLVAEIMPATSDSVPLIGQYFASIMIIVGMSVIATTVVLQYHHHDPNGGNMPKWVQLVLLQWVAWFLRMKR----PGESDDPERPPCAPH---LRRCS---SGSQSGSIPNPPDHTLHPLHPQSLAPLQTGPLHVGHPHLHTQSTTNNNGNLLYMGFQSIEDSSVLSEPVQRNVSAGPPRVAGSPPPHLPSQFCSSPPPPASNMDTVACPSTVSSGGGFGGGVGGCSTAGIGDPQLQAILEEVRYMADRFREQDEAESVANQWKFAGAVIDRLCLVAFSVFNIICTISILMSA 558

[Close]

BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Medaka
Match: si:ch211-39a7.1 (neuronal acetylcholine receptor subunit alpha-7-like [Source:NCBI gene;Acc:101160921])

HSP 1 Score: 196.823 bits (499), Expect = 9.418e-54
Identity = 165/598 (27.59%), Postives = 274/598 (45.82%), Query Frame = 3

Query:  234 LIFGLFLCRGTDTVNWPSVEKNLTVSILNNYQRYGIFGRPVSHSKELMKVQYGLQLIQLLDLDENKQILRTNCWSMYRWHDKLLEWNPAEHGGITEIRVFPDQIWTPDIKLYNFXXXXXXXXXXXXXXXKHTGKTLWVPQSIFKSTCAVEITYFPFDTQICMLEFGSWAYDIHQLDIIWWVPDGTNVSMPYIDFNDYLQSNEWRTDGEVDFQLKLEGDNKTQIKSVKKYRTRVYVSSNGQPINKHYPXXXXXXXXXXNSSFYVLILVIPCILLSSLTLVVFWLPPESPAKMMLGMNIXXXXXXXXXXXXESTPSAVKKFPLIGAYFCLNMIMITLSTFLAALVINYYFRGDFAGPLPILVRRIIVEGLGRLLLVRQHIPLPDETNLMDRVLTRAENGCIRKCRLKIPNLDSNKFLTSPVQTAEPPLQQDLSPRNTNPF------------INND---------ELAESNVWQTNVR------PLRSYSSVYRNTPQASMTARPNPIT--DTEDSP-----------LLMRCNTTLEND--VRELKRYIRMFVNRQKENNRKNLISMEWRTLALVLDRLFFVLYISSILIAVIACIPSS 1901
            L  GL     + ++  P  +++L  S+L +Y R     RPV++    + V + L LIQ++D+DE  Q+L +N W    W+D  L+WN +E+ G+  +R   DQ+WTPDI LYN AD          V+V  +G   ++P  IF STC V++ +FPFD Q C L+FGSW YD   LD+           M   D + Y+ + EW   G                  V   R  ++     +P    Y  + +++ +RR + +Y L L+IPC+LLSS+TL+VF LP +S  K+ LG+ + ++  V +LL+AE  P+     PLIG YF   MI++ +S     +V+ Y+      G +P  V+ ++++ +   L +++    P E++  +R         +R+C        S      P  T  P   Q L+P  T P              NN+          + +S+V    V+      P R   S   + P    ++ P P +  DT   P            +  C+T    D  ++ +   +R   +R +E +    ++ +W+    V+DRL  V +    +I  I+ + S+
Sbjct:   53 LFCGLTFSPSSVSLQGPH-QRSLLKSLLKDYNR---MERPVANDSHTLTVHFTLSLIQIMDVDEKNQVLTSNMWLQMTWYDHYLQWNQSEYPGVKNLRFTSDQVWTPDILLYNSADDDFDSTFKTNVLVNSSGYAKYLPPGIFMSTCNVDVRWFPFDIQKCNLKFGSWTYDGWLLDL----------QMIEADISSYMPNGEWDLIG------------------VPGSRNEIFYDCCKEP----YTDVTFVITIRRRTLYYALNLLIPCVLLSSMTLLVFVLPADSGEKISLGITVLLSLTVFMLLVAEIMPATSDSVPLIGQYFASIMIIVGMSVIATTVVLQYHHHDPNGGNMPKWVQLVLLQWVAWFLRMKR----PGESDDPERPPCAPH---LRRCS---SGSQSGSIPNPPDHTLHPLHPQSLAPLQTGPLHVGHPHLHTQSTTNNNGNLLYMGFQSIEDSSVLSEPVQRNVSAGPPRVAGSPPPHLPSQFCSSPPPPASNMDTVACPSTVSSGGGFGGGVGGCSTAGIGDPQLQAILEEVRYMADRFREQDEAESVANQWKFAGAVIDRLCLVAFSVFNIICTISILMSA 604

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BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Medaka
Match: ENSORLT00000017941.2 (neuronal acetylcholine receptor subunit alpha-3-like [Source:NCBI gene;Acc:101168601])

HSP 1 Score: 193.741 bits (491), Expect = 1.136e-53
Identity = 142/521 (27.26%), Postives = 245/521 (47.02%), Query Frame = 3

Query:  324 YQRYGIFGRPVSHSKELMKVQYGLQLIQLLDLDENKQILRTNCWSMYRWHDKLLEWNPAEHGGITEIRVFPDQIWTPDIKLYNFXXXXXXXXXXXXXXXKHTGKTLWVPQSIFKSTCAVEITYFPFDTQICMLEFGSWAYDIHQLDIIWWVPDGTNVSMPYIDFNDYLQSNEWRTDGEVDFQLKLEGDNKTQIKSVKKYRTRVYVSSNGQPINKHYPXXXXXXXXXXNSSFYVLILVIPCILLSSLTLVVFWLPPESPAKMMLGMNIXXXXXXXXXXXXESTPSAVKKFPLIGAYFCLNMIMITLSTFLAALVINYYFRGDFAGPLPILVRRIIVEGLGRLLLVRQHIPLPDETNLMDRVLTRAENGCIRKCRLKIPNLDSNKFLTSPVQTAEPPLQQDLSPRNTNPFINNDELAESNVWQTNVRPLRSYSSVYRNTPQASMTARPNPITDTEDSPLLMRCNTTLENDVRELKRYIRMFVNRQKENNRKNLISMEWRTLALVLDRLFFVLYISSILIAVIA 1886
            ++RY  F RPV +  + + V++ + + QL+ +DE  QI+ TN W  + W+D  L W PAE  GI  IRV  ++IW PDI LYN A          + ++K  G   WVP +IFKS+C ++ITYFPFD Q C ++FGSW YD  ++D+   V  G+ V++      D+ +S EW       ++  ++ +   +I                      YP + Y   +RR   FY + L+IPC+L+S LT++VF+LP +   K+ L +++ ++  V LL++ E+ PS     PLIG Y    MI +TLS  +   V+N ++R      +P  V  + ++ L R++L+R+  P  ++  +    L   E G    C+L   +    K    P Q      +   S R  +      +L++ ++                NT  A     P                     +V++    ++      +  N+   +  +W+ +A+V+DR+F  ++++  L+  + 
Sbjct:   37 FRRYNQFIRPVENVSDPVTVEFEVSISQLVKVDEVNQIMETNLWLRHIWNDYKLRWTPAEFDGIEFIRVPSNKIWRPDIVLYNNAVGDFLVEDKTKALLKFDGTITWVPPAIFKSSCPMDITYFPFDYQNCSMKFGSWTYDKAKIDL---VLIGSKVNL-----KDFWESGEWEIIDAPGYKHDIKYNCCEEI----------------------YPDITYSFYIRRLPLFYTINLIIPCLLISFLTVLVFYLPSDCGEKVTLCISVLLSLTVFLLVITETIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHYRTPMTHTMPGWVHLVFLKVLPRIMLMRR--PTDEDGKVGGGSLNCLEYG---DCKLSSMDGCGAKKCPCPCQHGRETPETAASVRMKH------QLSQQSI----------------NTVVAFSVVSP---------------------EVKQAIESVKYIAENMRSRNKAKEVEDDWKYVAMVIDRIFLWVFVTVCLLGTMG 479

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BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Medaka
Match: chrna2b (neuronal acetylcholine receptor subunit alpha-2 [Source:NCBI gene;Acc:101155076])

HSP 1 Score: 192.971 bits (489), Expect = 5.243e-53
Identity = 125/392 (31.89%), Postives = 206/392 (52.55%), Query Frame = 3

Query:  288 VEKNLTVSILNNYQRYGIFGRPVSHSKELMKVQYGLQLIQLLDLDENKQILRTNCWSMYRWHDKLLEWNPAEHGGITEIRVFPDQIWTPDIKLYNFXXXXXXXXXXXXXXXKHTGKTLWVPQSIFKSTCAVEITYFPFDTQICMLEFGSWAYDIHQLDIIWWVPDGTNVSMPYIDFNDYLQSNEWRTDGEVDFQLKLEGDNKTQIKSVKKYRTRVYVSSNGQPINKHYPXXXXXXXXXXNSSFYVLILVIPCILLSSLTLVVFWLPPESPAKMMLGMNIXXXXXXXXXXXXESTPSAVKKFPLIGAYFCLNMIMITLSTFLAALVINYYFRGDFAGPLPILVRRIIVEGLGRLLLVRQHIPLPDETNLMDRVLTRAENGCIRKCRLKIPNLD 1463
             E+ L  ++   Y ++    RPV +  +++ V++GL + QL+D+DE  Q++ TN W    W+D  L W P+++  +T IRV  + IW PDI LYN AD       + +  + HTGK  WVP +I+KS+C++++T+FPFD Q C ++FGSW YD  ++D+              +D N+Y +S EW                   I +V  Y T+ Y        ++ YP + Y   +RR   FY + L+IPC+L+S LT++VF+LP +   K+ L +++ ++  V LLL+ E  PS     PLIG Y    MI +TLS  +   V+N + R      +P  V  + ++ + R L +R+    P       R+L   ++G + + + ++  LD
Sbjct:   43 AEEELFQTLFTGYNKWS---RPVPNISDVVIVKFGLSIAQLIDVDEKNQMMTTNVWLKQEWNDYKLRWKPSDYDNVTSIRVPSELIWVPDIVLYNNADGEFAVTHMTKAHLFHTGKVRWVPPAIYKSSCSIDVTFFPFDQQNCKMKFGSWTYDKAKIDL--------ERIENTVDLNNYWESGEWAI-----------------INAVGTYNTKKY-----DCCHEIYPDITYFFIIRRLPLFYTINLIIPCLLISCLTVLVFYLPSDCGEKITLCISVLLSLTVFLLLITEIIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHHRSPSTHKMPRWVHTVFLDLIPRWLFMRR----PAPNGRRRRLLLLQQDGALGRLQPRVSALD 397

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Query:  288 VEKNLTVSILNNYQRYGIFGRPVSHSKELMKVQYGLQLIQLLDLDENKQILRTNCWSMYRWHDKLLEWNPAEHGGITEIRVFPDQIWTPDIKLYNFXXXXXXXXXXXXXXXKHTGKTLWVPQSIFKSTCAVEITYFPFDTQICMLEFGSWAYDIHQLDIIWWVPDGTNVSMPYIDFNDYLQSNEWRTDGEVDFQLKLEGDNKTQIKSVKKYRTRVYVSSNGQPINKHYPXXXXXXXXXXNSSFYVLILVIPCILLSSLTLVVFWLPPESPAKMMLGMNIXXXXXXXXXXXXESTPSAVKKFPLIGAYFCLNMIMITLSTFLAALVINYYFRGDFAGPLPILVRRIIVEGLGRLLLVRQHIPLPDETNLMDRVLTRAENGCIRKCRLKIPNLD 1463
             E+ L  ++   Y ++    RPV +  +++ V++GL + QL+D+DE  Q++ TN W    W+D  L W P+++  +T IRV  + IW PDI LYN AD       + +  + HTGK  WVP +I+KS+C++++T+FPFD Q C ++FGSW YD  ++D+              +D N+Y +S EW                   I +V  Y T+ Y        ++ YP + Y   +RR   FY + L+IPC+L+S LT++VF+LP +   K+ L +++ ++  V LLL+ E  PS     PLIG Y    MI +TLS  +   V+N + R      +P  V  + ++ + R L +R+    P       R+L   ++G + + + ++  LD
Sbjct:   43 AEEELFQTLFTGYNKWS---RPVPNISDVVIVKFGLSIAQLIDVDEKNQMMTTNVWLKQEWNDYKLRWKPSDYDNVTSIRVPSELIWVPDIVLYNNADGEFAVTHMTKAHLFHTGKVRWVPPAIYKSSCSIDVTFFPFDQQNCKMKFGSWTYDKAKIDL--------ERIENTVDLNNYWESGEWAI-----------------INAVGTYNTKKY-----DCCHEIYPDITYFFIIRRLPLFYTINLIIPCLLISCLTVLVFYLPSDCGEKITLCISVLLSLTVFLLLITEIIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHHRSPSTHKMPRWVHTVFLDLIPRWLFMRR----PAPNGRRRRLLLLQQDGALGRLQPRVSALD 397

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BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Planmine SMEST
Match: SMESG000021568.1 (SMESG000021568.1)

HSP 1 Score: 1065.06 bits (2753), Expect = 0.000e+0
Identity = 570/570 (100.00%), Postives = 570/570 (100.00%), Query Frame = 3

Query:  225 MIFLIFGLFLCRGTDTVNWPSVEKNLTVSILNNYQRYGIFGRPVSHSKELMKVQYGLQLIQLLDLDENKQILRTNCWSMYRWHDKLLEWNPAEHGGITEIRVFPDQIWTPDIKLYNFXXXXXXXXXXXXXXXKHTGKTLWVPQSIFKSTCAVEITYFPFDTQICMLEFGSWAYDIHQLDIIWWVPDGTNVSMPYIDFNDYLQSNEWRTDGEVDFQLKLEGDNKTQIKSVKKYRTRVYVSSNGQPINKHYPXXXXXXXXXXNSSFYVLILVIPCILLSSLTLVVFWLPPESPAKMMLGMNIXXXXXXXXXXXXESTPSAVKKFPLIGAYFCLNMIMITLSTFLAALVINYYFRGDFAGPLPILVRRIIVEGLGRLLLVRQHIPLPDETNLMDRVLTRAENGCIRKCRLKIPNLDSNKFLTSPVQTAEPPLQQDLSPRNTNPFINNDELAESNVWQTNVRPLRSYSSVYRNTPQASMTARPNPITDTEDSPLLMRCNTTLENDVRELKRYIRMFVNRQKENNRKNLISMEWRTLALVLDRLFFVLYISSILIAVIACIPSSTEPVIPAQYRD 1934
            MIFLIFGLFLCRGTDTVNWPSVEKNLTVSILNNYQRYGIFGRPVSHSKELMKVQYGLQLIQLLDLDENKQILRTNCWSMYRWHDKLLEWNPAEHGGITEIRVFPDQIWTPDIKLYNFADVRLREARVARVVVKHTGKTLWVPQSIFKSTCAVEITYFPFDTQICMLEFGSWAYDIHQLDIIWWVPDGTNVSMPYIDFNDYLQSNEWRTDGEVDFQLKLEGDNKTQIKSVKKYRTRVYVSSNGQPINKHYPVLRYLVRLRRNSSFYVLILVIPCILLSSLTLVVFWLPPESPAKMMLGMNIFVAFFVLLLLLAESTPSAVKKFPLIGAYFCLNMIMITLSTFLAALVINYYFRGDFAGPLPILVRRIIVEGLGRLLLVRQHIPLPDETNLMDRVLTRAENGCIRKCRLKIPNLDSNKFLTSPVQTAEPPLQQDLSPRNTNPFINNDELAESNVWQTNVRPLRSYSSVYRNTPQASMTARPNPITDTEDSPLLMRCNTTLENDVRELKRYIRMFVNRQKENNRKNLISMEWRTLALVLDRLFFVLYISSILIAVIACIPSSTEPVIPAQYRD
Sbjct:    1 MIFLIFGLFLCRGTDTVNWPSVEKNLTVSILNNYQRYGIFGRPVSHSKELMKVQYGLQLIQLLDLDENKQILRTNCWSMYRWHDKLLEWNPAEHGGITEIRVFPDQIWTPDIKLYNFADVRLREARVARVVVKHTGKTLWVPQSIFKSTCAVEITYFPFDTQICMLEFGSWAYDIHQLDIIWWVPDGTNVSMPYIDFNDYLQSNEWRTDGEVDFQLKLEGDNKTQIKSVKKYRTRVYVSSNGQPINKHYPVLRYLVRLRRNSSFYVLILVIPCILLSSLTLVVFWLPPESPAKMMLGMNIFVAFFVLLLLLAESTPSAVKKFPLIGAYFCLNMIMITLSTFLAALVINYYFRGDFAGPLPILVRRIIVEGLGRLLLVRQHIPLPDETNLMDRVLTRAENGCIRKCRLKIPNLDSNKFLTSPVQTAEPPLQQDLSPRNTNPFINNDELAESNVWQTNVRPLRSYSSVYRNTPQASMTARPNPITDTEDSPLLMRCNTTLENDVRELKRYIRMFVNRQKENNRKNLISMEWRTLALVLDRLFFVLYISSILIAVIACIPSSTEPVIPAQYRD 570

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BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Planmine SMEST
Match: SMESG000073518.1 (SMESG000073518.1)

HSP 1 Score: 458.759 bits (1179), Expect = 3.178e-153
Identity = 273/568 (48.06%), Postives = 385/568 (67.78%), Query Frame = 3

Query:  225 MIFLIFGLFLCRGTDTVNWPSVEKNLTVSILNNYQRYGIFGRPVSHSKELMKVQYGLQLIQLLDLDENKQILRTNCWSMYRWHDKLLEWNPAEHGGITEIRVFPDQIWTPDIKLYNFXXXXXXXXXXXXXXXKHTGKTLWVPQSIFKSTCAVEITYFPFDTQICMLEFGSWAYDIHQLDIIWWVPDGTNVSMPYIDFNDYLQSNEWRTDGEVDFQLKLEGDNKTQIKSVKKYRTRVYVSSNGQPINKHYPXXXXXXXXXXNSSFYVLILVIPCILLSSLTLVVFWLPPESPAKMMLGMNIXXXXXXXXXXXXESTPSAVKKFPLIGAYFCLNMIMITLSTFLAALVINYYFRGDFAGPLPILVRRIIVEGLGRLLLVRQHI--PLPDETNLMDRVLTRAENGCIRKCRLKIPNLDSNKFLTSPVQTAE-PPLQQDLSPRN--TNPFINNDELAESNVWQTNVRPLRSYSSVYRNTPQASMTARPNP-ITDTEDSPLLMRCNT-TLENDVRELKRYIRMFVNRQKENNRKNLISMEWRTLALVLDRLFFVLYISSILIAVIACIPSSTE 1907
             +F+   +F+    + ++  SVEKNL   ++ +Y+ YG+ GRPV+ S+  ++V YGL L+Q+L LD N QILRTNCW  Y WHD+LL W+P ++ GI +IR+  D +W PDI+L+NFAD R++  R ARVVV + G  LWVPQ++FKS+C+VEI+YFPFD QIC ++FGSW YD  QL++ W+     N S+PY+DF  Y+ +NEW TDGE +  +      K QIKS++  R +  ++   + I K + VLRY +RLRRN SFYV IL+IPCILLS L  +VFWLP ESPAK++LGMNIFVAFF+LLLLL  S P+AVK FPLIG++FCLNMIMITLSTFL  +VIN+Y RGD  GP+P  +R+I+++ +GRLL V Q I  P P   N  +  +T++  G I+  +L +P L  NKF+ + +   E     QDLS RN    P+  N   A+ N+ Q   R   +    +   P + +   PNP ++++ DSP+++   T ++E DV E+ RY+R ++ +QKE  RKN ++MEWRTL LVLDRLF + Y+++I+IAVI  IP++ +
Sbjct:    8 FVFIYAFVFINADDEFLSKKSVEKNLLQKLMKSYEFYGVNGRPVNDSRNKIRVNYGLALVQILGLDGNTQILRTNCWGRYTWHDELLNWDPKDYDGIDQIRLEKDYMWMPDIQLFNFADERMKVHREARVVVSNDGSILWVPQALFKSSCSVEISYFPFDVQICRMDFGSWTYDTSQLELHWY--GFPNDSLPYVDFKAYIHNNEWHTDGEKEKNISHLHRTK-QIKSLRFLRYQ-NITDGDEIIQKTFHVLRYRIRLRRNPSFYVFILIIPCILLSLLVNIVFWLPTESPAKLLLGMNIFVAFFILLLLLKSSIPTAVKAFPLIGSFFCLNMIMITLSTFLVTIVINFYVRGDRKGPMPNYIRKIMIDNIGRLLFVSQFIPLPPPQPPNNCNVYITQSPAGKIQH-KLYVPELTCNKFMATDIPNIEQTSTSQDLSNRNNINKPYSKNFISADKNLNQKYSR--NNGHRSHNQKPNSEINNIPNPGVSNSHDSPIMICGGTSSIEKDVIEITRYVRKYIGKQKEAQRKNQLTMEWRTLGLVLDRLFCIFYMATIVIAVIIGIPTNAD 568

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BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Planmine SMEST
Match: SMESG000023360.1 (SMESG000023360.1)

HSP 1 Score: 453.751 bits (1166), Expect = 6.524e-152
Identity = 276/544 (50.74%), Postives = 366/544 (67.28%), Query Frame = 3

Query:  279 WPSVEKNLTVSILNNYQRYGIFGRPVSHSKELMKVQYGLQLIQLLDLDENKQILRTNCWSMYRWHDKLLEWNPAEHGGITEIRVFPDQIWTPDIKLYNFXXXXXXXXXXXXXXXKHTGKTLWVPQSIFKSTCAVEITYFPFDTQICMLEFGSWAYDIHQLDIIWWV----PDGTNVSMPYIDFNDYLQSNEWRTDGEVDFQLKLEGDNKTQIKSVKKYRTRVYVSSNGQPINKHYPXXXXXXXXXXNSSFYVLILVIPCILLSSLTLVVFWLPPESPAKMMLGMNIXXXXXXXXXXXXESTPSAVKKFPLIGAYFCLNMIMITLSTFLAALVINYYFRGDFAGPLPILVRRIIVEGLGRLLLVRQHIPLPDETNLMDRVLTRAENGCIRKCRLKIPNLDSNKFLTSPVQTAEPPLQQDLSPRNTNPF-INNDELAESNVWQTNVRPLRSYSSVYRNTPQASMTARPNPITDTEDSPLLMRCNTTLENDVRELKRYIRMFVNRQKENNRKNLISMEWRTLALVLDRLFFVLYISSILIAVIACIP 1895
            + SVEK L  ++L  Y+R+G+ GRPV+ S  L+ V+YGL LIQ+L L+ENKQILRTNCWS Y W D LL+W+PA + GI +IR+FP +IWTPDIKL+NFAD RL E R ARVV+ + G  LW+PQ++FKSTC V ITYFPFDTQ+C LEFGSW YD  QL++ WW        T   +PY+D+ DY+ SNEW TDG+V+ ++K   D K QIKS KK R     +  GQ I + Y +L Y +R++RNSSFYV ILVIPC+LLS LTLV+FWLPPESPAKMMLGMNIFVAFF+LLLLLAESTPS+VK FPLIGAYFCLNMI+ITLSTFLAALV+N YFRGD  G +P ++RR+ ++GLG+L++  Q      E + + + +    N  I K + KI + + N  +      ++   Q +   R  N + I+ DE  E N                          + + I++             LE D+ ++K +I+ FVNRQK++  K+++S EWR+LAL+LDR+FF LY+++ILIA++  IP
Sbjct:   26 YKSVEKTLIRNLLTRYKRFGVIGRPVNQSDNLIVVKYGLALIQILGLEENKQILRTNCWSRYVWKDILLQWDPANYSGIKQIRIFPQEIWTPDIKLHNFADERLSEFREARVVISNDGNVLWIPQALFKSTCPVTITYFPFDTQVCKLEFGSWTYDTTQLNLDWWHGYNDETKTYYPIPYVDYTDYVDSNEWITDGQVEKKIK-SYDRKLQIKSEKKIRMITERNHLGQNITRRYHILEYGIRMKRNSSFYVFILVIPCLLLSCLTLVLFWLPPESPAKMMLGMNIFVAFFILLLLLAESTPSSVKNFPLIGAYFCLNMILITLSTFLAALVLNLYFRGDSKGRVPGIIRRLFIDGLGKLIMAGQQ-----EVSTIHQGILFTMN--IGKGKSKIKSTEKNFQINHAAFRSKMRKQLNTGNRGENEYEIDGDEKPELN--------------------------KKDGISN--------HSFNILEKDLSDIKSFIQEFVNRQKQHTMKSIVSQEWRSLALILDRMFFCLYVTAILIALVVLIP 527

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BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Planmine SMEST
Match: SMESG000005358.1 (SMESG000005358.1)

HSP 1 Score: 437.573 bits (1124), Expect = 1.004e-145
Identity = 265/553 (47.92%), Postives = 349/553 (63.11%), Query Frame = 3

Query:  231 FLIFGLFLCRGTDTVNWPSVEKNLTVSILNNYQRYGIFGRPVSHSKELMKVQYGLQLIQLLDLDENKQILRTNCWSMYRWHDKLLEWNPAEHGGITEIRVFPDQIWTPDIKLYNFXXXXXXXXXXXXXXXKHTGKTLWVPQSIFKSTCAVEITYFPFDTQICMLEFGSWAYDIHQLDIIWWVPDGTNVSMPYIDFNDYLQSNEWRTDGEVDFQLKLEGDNKTQIKSVKKYRTRVYVSSNGQPINKHYPXXXXXXXXXXNSSFYVLILVIPCILLSSLTLVVFWLPPESPAKMMLGMNIXXXXXXXXXXXXESTPSAVKKFPLIGAYFCLNMIMITLSTFLAALVINYYFRGDFAGPLPILVRRIIVEGLGRLLLVRQHIPLPDETNLMDRVLTRAENGCIRKCRLKIPNLDSNKFLTSPVQTAEPPLQQDLSPRNTNPFINNDELAESNVWQTNVRPLRSYSSVYRNTPQASMTARPNPITDTEDSPLLMRCNTTLENDVRELKRYIRMFVNRQKENNRKNLISMEWRTLALVLDRLFFVLYISSILIAVIAC 1889
             +IFGLF      T    S+EK+L  +++N Y+ +GIFGRPV++S E + +++ LQLIQ+LDLDEN+QIL TNCWS  RW D LL W+P ++G IT +RV+PD IWTPDI LYNFAD R  + R  RV +   G  LWVPQS++KS+C+VEITYFPFDTQ C LEFGS AYD  +LDIIWW+ +  N+S  YID+ DY++SNEW   G       ++     +I S K YR         + +   YP+LRY++RL RN SFY+ IL++PC LLS LTLV+FWLPPESPAK+MLG++IFVAFFVLLLLLA+ TP  VK FPLIGAYFCLNM+MIT STFLA L+IN +F+G   GP+P  +RRII+EGLGRL+L+RQ+I LP                         P  D N+                L  +NT+       +  S +++             RN     +     P  +       ++ +   E D+RE+ RYIR+ VNR+K    +NLI+ME+RTLAL+LDRLFF+LYIS++  +VI C
Sbjct:    7 LMIFGLFPVLNALT-EVKSIEKDLIRTLINKYEHHGIFGRPVNNSMETIVIEHKLQLIQILDLDENRQILVTNCWSNLRWKDSLLIWDPQKYGNITTVRVYPDHIWTPDIMLYNFADERSEDRRECRVKIYANGMVLWVPQSLYKSSCSVEITYFPFDTQNCKLEFGSLAYDSTKLDIIWWITE--NISENYIDYADYMKSNEWEIYGS-----SIDSSITEKIFSKKIYRFSQISEKESKLL---YPILRYILRLNRNPSFYITILIVPCTLLSLLTLVIFWLPPESPAKLMLGISIFVAFFVLLLLLADFTPREVKNFPLIGAYFCLNMVMITSSTFLATLIINIHFKGAKTGPIPKTIRRIILEGLGRLVLIRQNINLP-------------------------PYNDGNENCIKKSSLKVKSKNSQLWIKNTDKRFRKRSINSSVIYKP------------RNDHSKKLIPIAEPCLNA-----FIQGHLEFERDIREISRYIRIIVNREKTEISRNLIAMEYRTLALILDRLFFILYISTMATSVIVC 506

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BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Planmine SMEST
Match: SMESG000069967.1 (SMESG000069967.1)

HSP 1 Score: 300.827 bits (769), Expect = 1.369e-92
Identity = 225/584 (38.53%), Postives = 331/584 (56.68%), Query Frame = 3

Query:  225 MIFLIFGLFLCRGTDTVNWPSVEKNLTVSILNNYQRYGIFGRPVSHSKELMKVQYGLQLIQLLDLDENKQILRTNCWSMYRWHDKLLEWNPAEHGGITEIRVFPDQIWTPDIKLYNFXXXXXXXXXXXXXXXKHTGKTLWVPQSIFKSTCAVEITYFPFDTQICMLEFGSWAYDIHQLDIIWWVPDGTNVSMPYIDFNDYLQSNEWRT--DGEVDFQLKLEGDNKTQIKSVKKYRTRVYVSSNGQPINKHYPXXXXXXXXXXNSSFYVLILVIPCILLSSLTLVVFWLPPESPAKMMLGMNIXXXXXXXXXXXXESTPSAVKKFPLIGAYFCLNMIMITLSTFLAALVINYYFRGDFAGPLPILVRRIIVEGLGRLLLVRQHI--PLPDETNLMDRVLTRAENGCIRKCRLKIPNLDSNKFLTSPV------QTAEPPLQ-QDLSPRNTN-------PFINND-----ELAESNVWQTNVRPLRSYSSVYRNTPQASMTARPNPITDTEDSPLLMRCNTT---LENDVRELKRYIRMFVNRQKENNRKNLISMEWRTLALVLDRLFF-VLYISSILIAVIACIP 1895
            M  LI  + L  G D  +  S EK L   +L +Y++ G  GRPV + ++ + V+YGL LIQ++ LDE  QIL  N W+ Y W D++L WNP     + E+R+ PD+IWTPDI LYN+AD RL E R   V V + G+ +W+P +IFKSTC ++I+ FPFD Q C L+FGSW Y   +LDI  +     N S   ID  DY +SNEW         F LK   DN +Q                        P L + + L+RNS+++  +LV+PC+LLS LTLV+FWLPPESPAKM+LGMNIFVAF +LL LLAE+TPSA    P +G Y+CLNMI+ITLSTFL+ +VIN YFR D    LP  +R+  V    ++L ++Q I     ++ +L+   +  + N   R+   +    +S + + +P+      ++++  ++ Q+LS  ++N       P  N D       A S++ + +  P  SY   Y N P  +  A P P + T+      + N +   LE ++ E+++ ++  + +  E +R N I+ EWR +A+VLDRLFF V ++  IL  +I  +P
Sbjct:    1 MDILIVFMILLLGVDYASM-SGEKKLITKMLKDYKKKGKIGRPVRNVQDTVVVKYGLALIQIMSLDEKNQILTVNVWAKYTWVDEMLIWNPKNFSNVEEVRMPPDKIWTPDIVLYNYADERLIEKRDVMVSVNYIGRIIWMPPAIFKSTCGIDISNFPFDFQSCYLKFGSWTYKGDKLDIQLY----ENFSE--IDIIDYTESNEWLILHKPARRFVLKNMKDNTSQ------------------------PDLTFFLILKRNSAYHAYLLVVPCVLLSLLTLVIFWLPPESPAKMILGMNIFVAFSLLLRLLAETTPSASTSVPFLGYYYCLNMILITLSTFLSIIVINLYFRCDKRRNLPDWLRK-GVNATSKILGMKQVISSASSEKLHLIKSEIKNSRNHIKRQQEKE--TTESRETIPTPISPRARHKSSKMAMEMQNLSGSSSNYQTRFQYPRQNTDVTDIPYTAHSDIPRNSFNPGYSYEYRYSNAPPPAAPASPLPSSTTKKQTQQKKSNQSAILLEKNLSEIRKALKNLMTKIAEKDRGNRIAKEWRMVAMVLDRLFFWVYFVIVILSGLILLLP 550

The following BLAST results are available for this feature:

BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Human
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Celegans
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Fly
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Zebrafish
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Xenopus
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Mouse
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. UniProt/SwissProt
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. TrEMBL
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Cavefish
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Sea Lamprey
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Yeast
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Nematostella
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Medaka
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Planmine SMEST

BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
CHRNA4	6.337e-54	33.80	cholinergic receptor nicotinic alpha 4 subunit [So...	[more]
CHRNA5	7.330e-54	33.77	cholinergic receptor nicotinic alpha 5 subunit [So...	[more]
CHRNB3	8.124e-53	32.98	cholinergic receptor nicotinic beta 3 subunit [Sou...	[more]
CHRNA2	1.771e-51	27.32	cholinergic receptor nicotinic alpha 2 subunit [So...	[more]
CHRNA2	4.261e-51	27.32	cholinergic receptor nicotinic alpha 2 subunit [So...	[more]

BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5


Match Name	E-value	Identity	Description
acr-11	3.010e-49	31.64	AcetylCholine Receptor [Source:UniProtKB/TrEMBL;A...	[more]
unc-29	3.759e-48	27.29	Acetylcholine receptor subunit beta-type unc-29 [...	[more]
unc-29	3.759e-48	27.29	Acetylcholine receptor subunit beta-type unc-29 [...	[more]
acr-3	5.468e-48	29.58	Acetylcholine receptor subunit beta-type acr-3 [S...	[more]
lev-1	6.484e-47	30.20	Acetylcholine receptor subunit beta-type lev-1 [S...	[more]

BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5


Match Name	E-value	Identity	Description
nAChRalpha2	4.579e-56	28.45	gene:FBgn0000039 transcript:FBtr0084639	[more]
nAChRalpha2	4.579e-56	28.45	gene:FBgn0000039 transcript:FBtr0084640	[more]
nAChRalpha6	5.122e-55	28.78	gene:FBgn0032151 transcript:FBtr0331284	[more]
nAChRalpha6	2.711e-54	28.60	gene:FBgn0032151 transcript:FBtr0303898	[more]
nAChRalpha6	2.711e-54	28.60	gene:FBgn0032151 transcript:FBtr0079916	[more]

BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
chrna2a	5.527e-57	29.58	cholinergic receptor, nicotinic, alpha 2a (neurona...	[more]
chrna2b	5.538e-54	33.99	cholinergic receptor, nicotinic, alpha 2b (neurona...	[more]
chrna3	1.960e-53	25.56	cholinergic receptor, nicotinic, alpha 3 [Source:Z...	[more]
chrnb3a	5.157e-53	33.70	cholinergic receptor, nicotinic, beta polypeptide ...	[more]
chrnb3a	5.722e-53	33.70	cholinergic receptor, nicotinic, beta polypeptide ...	[more]

BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5


Match Name	E-value	Identity	Description
CHRNA2	2.947e-57	28.10	cholinergic receptor, nicotinic alpha 2 [Source:NC...	[more]
CHRNA2	1.126e-55	34.43	cholinergic receptor, nicotinic alpha 2 [Source:NC...	[more]
rpap1	5.602e-53	34.17	RNA polymerase II associated protein 1 [Source:Xen...	[more]
rpap1	7.715e-53	35.83	RNA polymerase II associated protein 1 [Source:Xen...	[more]
rpap1	8.237e-53	35.92	RNA polymerase II associated protein 1 [Source:Xen...	[more]

BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Chrna2	6.487e-53	33.16	cholinergic receptor, nicotinic, alpha polypeptide...	[more]
Chrna2	6.487e-53	33.16	cholinergic receptor, nicotinic, alpha polypeptide...	[more]
Chrna6	7.926e-53	25.72	cholinergic receptor, nicotinic, alpha polypeptide...	[more]
Chrna4	1.071e-52	34.01	cholinergic receptor, nicotinic, alpha polypeptide...	[more]
Chrna4	1.472e-52	34.01	cholinergic receptor, nicotinic, alpha polypeptide...	[more]

BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|P23414\|ACH1_SCHGR	2.333e-56	35.85	Acetylcholine receptor subunit alpha-L1 OS=Schisto...	[more]
sp\|P17644\|ACH2_DROME	4.590e-55	28.45	Acetylcholine receptor subunit alpha-like 2 OS=Dro...	[more]
sp\|P43679\|ACHB3_CHICK	1.469e-54	34.55	Neuronal acetylcholine receptor subunit beta-3 OS=...	[more]
sp\|P09481\|ACHA3_CHICK	2.409e-54	27.29	Neuronal acetylcholine receptor subunit alpha-3 OS...	[more]
sp\|Q5IS77\|ACHA4_PANTR	1.976e-53	33.80	Neuronal acetylcholine receptor subunit alpha-4 OS...	[more]

BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A4Z2D9U5	0.000e+0	56.67	Neuronal acetylcholine receptor subunit alpha-2 OS...	[more]
A0A5K3FFX3	0.000e+0	55.54	Uncharacterized protein OS=Mesocestoides corti OX=...	[more]
A0A5K3FBT6	0.000e+0	55.54	Uncharacterized protein OS=Mesocestoides corti OX=...	[more]
A0A3Q0KEK5	0.000e+0	56.33	Putative nachr subunit OS=Schistosoma mansoni OX=6...	[more]
A0A068Y539	0.000e+0	54.27	Neuronal acetylcholine receptor subunit alpha 4 OS...	[more]

BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
chrna2a	3.576e-58	27.53	cholinergic receptor, nicotinic, alpha 2a (neurona...	[more]
chrna4b	1.379e-54	34.39	neuronal acetylcholine receptor subunit alpha-2 [S...	[more]
chrna4b	4.640e-54	34.39	neuronal acetylcholine receptor subunit alpha-2 [S...	[more]
chrna2b	1.634e-53	32.97	neuronal acetylcholine receptor subunit alpha-2 [S...	[more]
chrnb3b	1.806e-53	34.04	neuronal acetylcholine receptor subunit non-alpha-...	[more]

BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5


Match Name	E-value	Identity	Description
chrnb3b	3.777e-55	35.04	cholinergic receptor, nicotinic, beta 3b (neuronal...	[more]
chrna3	3.897e-52	34.49	cholinergic receptor, nicotinic, alpha 3 [Source:Z...	[more]
chrna2a	7.566e-50	34.20	cholinergic receptor, nicotinic, alpha 2a (neurona...	[more]
chrnb1l	2.976e-35	26.93	cholinergic receptor, nicotinic, beta 1 (muscle) l...	[more]
ENSPMAT00000008550.1	3.889e-35	25.93	pep scaffold:Pmarinus_7.0:GL476601:34:38373:1 gene...	[more]

BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5


Match Name	E-value	Identity	Description
EDO40862	5.757e-43	31.52	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO42054	7.018e-43	31.01	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO42072	8.252e-43	31.22	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO43642	2.718e-42	33.97	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO47969	5.457e-42	29.48	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5


Match Name	E-value	Identity	Description
si:ch211-39a7.1	5.319e-54	27.46	neuronal acetylcholine receptor subunit alpha-7-li...	[more]
si:ch211-39a7.1	9.418e-54	27.59	neuronal acetylcholine receptor subunit alpha-7-li...	[more]
ENSORLT00000017941.2	1.136e-53	27.26	neuronal acetylcholine receptor subunit alpha-3-li...	[more]
chrna2b	5.243e-53	31.89	neuronal acetylcholine receptor subunit alpha-2 [S...	[more]
chrna2b	5.243e-53	31.89	neuronal acetylcholine receptor subunit alpha-2 [S...	[more]

BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5


Match Name	E-value	Identity	Description
SMESG000021568.1	0.000e+0	100.00	SMESG000021568.1	[more]
SMESG000073518.1	3.178e-153	48.06	SMESG000073518.1	[more]
SMESG000023360.1	6.524e-152	50.74	SMESG000023360.1	[more]
SMESG000005358.1	1.004e-145	47.92	SMESG000005358.1	[more]
SMESG000069967.1	1.369e-92	38.53	SMESG000069967.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30029822 ID=SMED30029822|Name=Neuronal acetylcholine receptor subunit alpha-4|organism=Schmidtea mediterranea sexual|type=transcript|length=2260bp
ATAAACTTGTAAAACTAGACACCAGATTAGAATTTAATTTAATAAACATA
TTGATCAATGTTTTGCTGCCGCATACAGACTGATGCCATGCAAAATTCCT
TCCATGAAATTGTCTATAACTGACTGACATAGATCAATAATTTTCTTTCC
TCATCGGCCAATCAAAATTCATCAGGAAAATTATTATTACTATTCTCAGC
TCAATTCAACTGCGTTAAATCTGGATGATTTTTTTGATTTTTGGATTGTT
TCTCTGTCGAGGAACCGATACTGTAAACTGGCCGTCCGTTGAAAAGAACT
TGACAGTTTCGATTTTGAACAACTATCAACGCTATGGGATTTTTGGTCGG
CCAGTTAGTCACAGCAAGGAACTGATGAAAGTTCAATATGGGTTACAATT
GATACAGCTTCTCGATCTAGATGAGAACAAGCAAATATTAAGGACGAATT
GTTGGTCAATGTATAGATGGCATGACAAGCTGCTTGAATGGAATCCTGCC
GAACATGGTGGAATTACGGAGATCAGAGTTTTTCCAGATCAGATATGGAC
CCCAGATATCAAGCTTTATAACTTTGCCGATGTTCGATTGAGAGAGGCCC
GGGTCGCTCGAGTGGTCGTCAAGCATACTGGTAAAACGTTATGGGTTCCG
CAATCCATTTTCAAGAGCACTTGCGCTGTTGAGATTACTTATTTTCCATT
TGACACGCAAATCTGCATGCTTGAGTTTGGCTCCTGGGCATATGACATCC
ACCAGTTAGATATCATCTGGTGGGTTCCCGATGGCACCAATGTCTCGATG
CCTTATATTGATTTCAATGACTATTTACAAAGCAATGAATGGCGGACTGA
TGGAGAGGTGGATTTTCAGCTGAAGCTGGAAGGCGATAATAAAACACAGA
TTAAGTCCGTAAAGAAATACCGGACCCGGGTTTACGTAAGCAGTAATGGA
CAGCCTATAAATAAACACTATCCTGTTTTACGCTACCTGGTTCGGCTAAG
AAGAAATTCAAGTTTTTATGTACTGATATTGGTTATCCCATGCATTTTAT
TGTCTAGTCTCACTCTTGTAGTGTTTTGGCTACCCCCAGAATCACCGGCC
AAAATGATGTTAGGAATGAACATTTTCGTTGCATTTTTTGTGCTCCTCTT
GCTTTTAGCTGAATCAACGCCGAGTGCAGTGAAAAAATTTCCACTGATCG
GTGCCTATTTCTGTCTCAATATGATTATGATTACTTTGTCAACATTCCTA
GCAGCTCTGGTCATCAACTACTATTTCCGGGGTGACTTCGCAGGTCCGCT
GCCTATTCTAGTGAGGCGGATTATCGTGGAAGGTCTAGGCCGTTTATTGC
TCGTACGGCAGCACATACCTCTGCCAGACGAAACCAATTTAATGGACCGA
GTATTAACTCGTGCAGAAAATGGATGCATTCGTAAATGCAGATTAAAAAT
CCCCAATTTGGATTCCAATAAATTTCTCACGTCACCCGTGCAAACTGCTG
AACCGCCTTTACAACAAGATTTATCCCCACGAAATACGAATCCTTTCATC
AATAATGATGAATTAGCTGAAAGCAATGTCTGGCAGACAAATGTGAGACC
GTTACGGTCATACTCTTCAGTGTACAGAAATACTCCTCAAGCATCTATGA
CCGCCCGACCGAATCCCATTACCGATACCGAGGATTCGCCTCTGCTTATG
AGGTGTAACACTACCCTAGAAAATGACGTGCGTGAATTGAAACGTTACAT
ACGTATGTTTGTCAACAGGCAAAAAGAAAATAATAGAAAGAATTTGATTT
CAATGGAATGGAGAACCTTAGCTTTAGTACTTGATAGATTGTTCTTTGTT
TTATACATAAGCTCTATTCTCATCGCTGTAATTGCCTGCATTCCGAGTTC
CACGGAGCCCGTAATTCCGGCTCAGTACCGAGATTGAAACCTTTCATAAA
CAAAGTTTTCTACTTAGTGTAGAATTTAGAATTTGCACCTGAAAAACTAA
ATGAGTGATCTCTGTTCTGGGGTGCAGTTAATTGGCTTTTCCAGTAGTTT
GTTGTTCATAATATGTAGATATTATTGTAAATAAACATTGAGCAATCTTT
CGGAGAACTGATTTAGAAAATTAAAACAAATCAAACATTCTCCTAAATTC
AGTTACAATATTACATCAAAGATCAAAAATTTCAATTTTATTGTATATTA
CATAATCCAATACAATTTGTCGATGATTATGCAAACTGTATTAATTAAAA
AGTCCTACAA

protein sequence of SMED30029822-orf-1

>SMED30029822-orf-1 ID=SMED30029822-orf-1|Name=SMED30029822-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=571bp
MIFLIFGLFLCRGTDTVNWPSVEKNLTVSILNNYQRYGIFGRPVSHSKEL
MKVQYGLQLIQLLDLDENKQILRTNCWSMYRWHDKLLEWNPAEHGGITEI
RVFPDQIWTPDIKLYNFADVRLREARVARVVVKHTGKTLWVPQSIFKSTC
AVEITYFPFDTQICMLEFGSWAYDIHQLDIIWWVPDGTNVSMPYIDFNDY
LQSNEWRTDGEVDFQLKLEGDNKTQIKSVKKYRTRVYVSSNGQPINKHYP
VLRYLVRLRRNSSFYVLILVIPCILLSSLTLVVFWLPPESPAKMMLGMNI
FVAFFVLLLLLAESTPSAVKKFPLIGAYFCLNMIMITLSTFLAALVINYY
FRGDFAGPLPILVRRIIVEGLGRLLLVRQHIPLPDETNLMDRVLTRAENG
CIRKCRLKIPNLDSNKFLTSPVQTAEPPLQQDLSPRNTNPFINNDELAES
NVWQTNVRPLRSYSSVYRNTPQASMTARPNPITDTEDSPLLMRCNTTLEN
DVRELKRYIRMFVNRQKENNRKNLISMEWRTLALVLDRLFFVLYISSILI
AVIACIPSSTEPVIPAQYRD*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **molecular function**
Term	Definition
GO:0004888	transmembrane signaling receptor activity
GO:0005216	ion channel activity
GO:0004889	acetylcholine-activated cation-selective channel activity
GO:0005230	extracellular ligand-gated ion channel activity
GO:0022848	acetylcholine-gated cation-selective channel activity

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000099	neuron
PLANA:0000101	muscle cell
PLANA:0000140	anterior region of the whole animal
PLANA:0000418	head

Vocabulary: **INTERPRO**
Term	Definition
IPR036734	Neur_chan_lig-bd_sf
IPR036719	Neuro-gated_channel_TM_sf
IPR002394	Nicotinic_acetylcholine_rcpt
IPR006201	Neur_channel
IPR006029	Neurotrans-gated_channel_TM
IPR006202	Neur_chan_lig-bd
IPR027361	Acetylcholine_rcpt_TM
IPR018000	Neurotransmitter_ion_chnl_CS

Vocabulary: **biological process**
Term	Definition
GO:0006811	ion transport
GO:0006810	transport
GO:0034220	ion transmembrane transport
GO:0060078	regulation of postsynaptic membrane potential
GO:0060079	excitatory postsynaptic potential

Vocabulary: **cellular component**
Term	Definition
GO:0016021	integral component of membrane
GO:0045211	postsynaptic membrane
GO:0016020	membrane
GO:0005886	plasma membrane
GO:0030054	cell junction
GO:0045202	synapse

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002394	Nicotinic acetylcholine receptor	PRINTS	PR00254	NICOTINICR	coord: 130..148 score: 42.22 coord: 94..108 score: 37.48 coord: 60..76 score: 41.76
IPR006201	Neurotransmitter-gated ion-channel	PRINTS	PR00252	NRIONCHANNEL	coord: 106..117 score: 49.1 coord: 150..164 score: 47.09 coord: 256..268 score: 29.86 coord: 73..89 score: 29.7
IPR006201	Neurotransmitter-gated ion-channel	PANTHER	PTHR18945	NEUROTRANSMITTER GATED ION CHANNEL	coord: 33..560
IPR036734	Neurotransmitter-gated ion-channel ligand-binding domain superfamily	GENE3D	G3DSA:2.70.170.10		coord: 22..229 e-value: 1.8E-52 score: 179.8
IPR036734	Neurotransmitter-gated ion-channel ligand-binding domain superfamily	SUPERFAMILY	SSF63712	Nicotinic receptor ligand binding domain-like	coord: 27..208
None	No IPR available	GENE3D	G3DSA:1.20.58.390		coord: 230..353 e-value: 2.4E-32 score: 113.6
None	No IPR available	PANTHER	PTHR18945:SF778	ACETYLCHOLINE RECEPTOR SUBUNIT BETA-LIKE 2	coord: 33..560
None	No IPR available	CDD	cd19051	LGIC_TM_cation	coord: 263..355 e-value: 1.13897E-23 score: 93.9664
None	No IPR available	CDD	cd19033	LGIC_ECD_nAChR_proto-like	coord: 67..261 e-value: 1.08345E-81 score: 251.822
None	No IPR available	SIGNALP_EUK	SignalP-noTM	SignalP-noTM	coord: 1..16 score: 0.743
None	No IPR available	TMHMM	TMhelix		coord: 264..286
None	No IPR available	TMHMM	TMhelix		coord: 325..347
None	No IPR available	TMHMM	TMhelix		coord: 295..312
None	No IPR available	TMHMM	TMhelix		coord: 539..561
IPR006202	Neurotransmitter-gated ion-channel ligand-binding domain	PFAM	PF02931	Neur_chan_LBD	coord: 25..213 e-value: 1.4E-48 score: 165.2
IPR006029	Neurotransmitter-gated ion-channel transmembrane domain	PFAM	PF02932	Neur_chan_memb	coord: 269..550 e-value: 5.9E-31 score: 108.3
IPR018000	Neurotransmitter-gated ion-channel, conserved site	PROSITE	PS00236	NEUROTR_ION_CHANNEL	coord: 150..164
IPR036719	Neurotransmitter-gated ion-channel transmembrane domain superfamily	SUPERFAMILY	SSF90112	Neurotransmitter-gated ion-channel transmembrane pore	coord: 263..553

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Neuronal acetylcholine receptor subunit alpha-4

Zeng et. al., 2018

▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 12

Owlery

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