Polyubiquitin
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30029792 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 5
Homology
BLAST of Polyubiquitin vs. Ensembl Human
Match: UBB (ubiquitin B [Source:HGNC Symbol;Acc:HGNC:12463]) HSP 1 Score: 89.7373 bits (221), Expect = 3.438e-21 Identity = 52/152 (34.21%), Postives = 89/152 (58.55%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVN 512
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+
Sbjct: 75 GGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLR 224
BLAST of Polyubiquitin vs. Ensembl Human
Match: UBB (ubiquitin B [Source:HGNC Symbol;Acc:HGNC:12463]) HSP 1 Score: 89.7373 bits (221), Expect = 3.438e-21 Identity = 52/152 (34.21%), Postives = 89/152 (58.55%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVN 512
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+
Sbjct: 75 GGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLR 224
BLAST of Polyubiquitin vs. Ensembl Human
Match: UBB (ubiquitin B [Source:HGNC Symbol;Acc:HGNC:12463]) HSP 1 Score: 89.7373 bits (221), Expect = 3.438e-21 Identity = 52/152 (34.21%), Postives = 89/152 (58.55%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVN 512
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+
Sbjct: 75 GGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLR 224
BLAST of Polyubiquitin vs. Ensembl Human
Match: UBB (ubiquitin B [Source:HGNC Symbol;Acc:HGNC:12463]) HSP 1 Score: 89.7373 bits (221), Expect = 3.438e-21 Identity = 52/152 (34.21%), Postives = 89/152 (58.55%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVN 512
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+
Sbjct: 75 GGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLR 224
BLAST of Polyubiquitin vs. Ensembl Human
Match: UBC (ubiquitin C [Source:HGNC Symbol;Acc:HGNC:12468]) HSP 1 Score: 89.3521 bits (220), Expect = 4.148e-21 Identity = 52/152 (34.21%), Postives = 89/152 (58.55%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVN 512
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+
Sbjct: 75 GGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLR 224
BLAST of Polyubiquitin vs. Ensembl Celegans
Match: ubq-1 (Polyubiquitin-A Ubiquitin Ubiquitin-related [Source:UniProtKB/Swiss-Prot;Acc:P0CG71]) HSP 1 Score: 92.0485 bits (227), Expect = 3.158e-21 Identity = 53/161 (32.92%), Postives = 94/161 (58.39%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVNPSKNVFLYI 539
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V LIG+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+ + +++
Sbjct: 303 GGMQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLIGKTITLEVEAS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFV 461 HSP 2 Score: 90.1225 bits (222), Expect = 1.270e-20 Identity = 52/161 (32.30%), Postives = 93/161 (57.76%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVNPSKNVFLYI 539
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+ + +++
Sbjct: 75 GGMQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEAS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFV 233 HSP 3 Score: 90.1225 bits (222), Expect = 1.270e-20 Identity = 52/161 (32.30%), Postives = 93/161 (57.76%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVNPSKNVFLYI 539
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+ + +++
Sbjct: 227 GGMQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEAS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFV 385 HSP 4 Score: 72.4034 bits (176), Expect = 1.407e-14 Identity = 44/128 (34.38%), Postives = 74/128 (57.81%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAV 440
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ V
Sbjct: 379 GGMQIFVKTLIGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEAS--DTIENVKAKIQDKEGIPPDQQRLIFAGV 504
BLAST of Polyubiquitin vs. Ensembl Celegans
Match: ubq-1 (Polyubiquitin-A Ubiquitin Ubiquitin-related [Source:UniProtKB/Swiss-Prot;Acc:P0CG71]) HSP 1 Score: 92.0485 bits (227), Expect = 3.158e-21 Identity = 53/161 (32.92%), Postives = 94/161 (58.39%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVNPSKNVFLYI 539
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V LIG+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+ + +++
Sbjct: 303 GGMQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLIGKTITLEVEAS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFV 461 HSP 2 Score: 90.1225 bits (222), Expect = 1.270e-20 Identity = 52/161 (32.30%), Postives = 93/161 (57.76%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVNPSKNVFLYI 539
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+ + +++
Sbjct: 75 GGMQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEAS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFV 233 HSP 3 Score: 90.1225 bits (222), Expect = 1.270e-20 Identity = 52/161 (32.30%), Postives = 93/161 (57.76%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVNPSKNVFLYI 539
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+ + +++
Sbjct: 227 GGMQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEAS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFV 385 HSP 4 Score: 72.4034 bits (176), Expect = 1.407e-14 Identity = 44/128 (34.38%), Postives = 74/128 (57.81%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAV 440
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ V
Sbjct: 379 GGMQIFVKTLIGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEAS--DTIENVKAKIQDKEGIPPDQQRLIFAGV 504
BLAST of Polyubiquitin vs. Ensembl Celegans
Match: ubq-1 (Polyubiquitin-A Ubiquitin Ubiquitin-related [Source:UniProtKB/Swiss-Prot;Acc:P0CG71]) HSP 1 Score: 90.1225 bits (222), Expect = 1.551e-20 Identity = 52/161 (32.30%), Postives = 93/161 (57.76%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVNPSKNVFLYI 539
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+ + +++
Sbjct: 75 GGMQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEAS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFV 233 HSP 2 Score: 90.1225 bits (222), Expect = 1.551e-20 Identity = 52/161 (32.30%), Postives = 93/161 (57.76%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVNPSKNVFLYI 539
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+ + +++
Sbjct: 227 GGMQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEAS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFV 385 HSP 3 Score: 90.1225 bits (222), Expect = 1.551e-20 Identity = 52/161 (32.30%), Postives = 93/161 (57.76%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVNPSKNVFLYI 539
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+ + +++
Sbjct: 455 GGMQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEAS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFV 613 HSP 4 Score: 90.1225 bits (222), Expect = 1.551e-20 Identity = 52/161 (32.30%), Postives = 93/161 (57.76%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVNPSKNVFLYI 539
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+ + +++
Sbjct: 607 GGMQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEAS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFV 765 HSP 5 Score: 89.7373 bits (221), Expect = 2.124e-20 Identity = 52/152 (34.21%), Postives = 89/152 (58.55%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVN 512
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+
Sbjct: 683 GGMQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEAS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLR 832 HSP 6 Score: 88.5817 bits (218), Expect = 4.508e-20 Identity = 52/161 (32.30%), Postives = 93/161 (57.76%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVNPSKNVFLYI 539
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+ + +++
Sbjct: 379 GGMQIFVKTLIGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEAS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFV 537
BLAST of Polyubiquitin vs. Ensembl Celegans
Match: ubq-1 (Polyubiquitin-A Ubiquitin Ubiquitin-related [Source:UniProtKB/Swiss-Prot;Acc:P0CG71]) HSP 1 Score: 90.1225 bits (222), Expect = 1.551e-20 Identity = 52/161 (32.30%), Postives = 93/161 (57.76%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVNPSKNVFLYI 539
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+ + +++
Sbjct: 75 GGMQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEAS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFV 233 HSP 2 Score: 90.1225 bits (222), Expect = 1.551e-20 Identity = 52/161 (32.30%), Postives = 93/161 (57.76%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVNPSKNVFLYI 539
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+ + +++
Sbjct: 227 GGMQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEAS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFV 385 HSP 3 Score: 90.1225 bits (222), Expect = 1.551e-20 Identity = 52/161 (32.30%), Postives = 93/161 (57.76%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVNPSKNVFLYI 539
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+ + +++
Sbjct: 455 GGMQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEAS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFV 613 HSP 4 Score: 90.1225 bits (222), Expect = 1.551e-20 Identity = 52/161 (32.30%), Postives = 93/161 (57.76%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVNPSKNVFLYI 539
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+ + +++
Sbjct: 607 GGMQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEAS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFV 765 HSP 5 Score: 89.7373 bits (221), Expect = 2.124e-20 Identity = 52/152 (34.21%), Postives = 89/152 (58.55%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVN 512
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+
Sbjct: 683 GGMQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEAS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLR 832 HSP 6 Score: 88.5817 bits (218), Expect = 4.508e-20 Identity = 52/161 (32.30%), Postives = 93/161 (57.76%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVNPSKNVFLYI 539
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+ + +++
Sbjct: 379 GGMQIFVKTLIGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEAS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFV 537
BLAST of Polyubiquitin vs. Ensembl Fly
Match: Ubi-p5E (gene:FBgn0086558 transcript:FBtr0345892) HSP 1 Score: 89.3521 bits (220), Expect = 2.636e-20 Identity = 52/161 (32.30%), Postives = 93/161 (57.76%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVNPSKNVFLYI 539
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+ + +++
Sbjct: 75 GGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFV 233 HSP 2 Score: 89.3521 bits (220), Expect = 2.636e-20 Identity = 52/161 (32.30%), Postives = 93/161 (57.76%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVNPSKNVFLYI 539
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+ + +++
Sbjct: 227 GGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFV 385 HSP 3 Score: 89.3521 bits (220), Expect = 2.819e-20 Identity = 52/152 (34.21%), Postives = 89/152 (58.55%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVN 512
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+
Sbjct: 379 GGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLR 528
BLAST of Polyubiquitin vs. Ensembl Fly
Match: Ubi-p5E (gene:FBgn0086558 transcript:FBtr0070933) HSP 1 Score: 89.3521 bits (220), Expect = 2.636e-20 Identity = 52/161 (32.30%), Postives = 93/161 (57.76%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVNPSKNVFLYI 539
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+ + +++
Sbjct: 75 GGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFV 233 HSP 2 Score: 89.3521 bits (220), Expect = 2.636e-20 Identity = 52/161 (32.30%), Postives = 93/161 (57.76%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVNPSKNVFLYI 539
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+ + +++
Sbjct: 227 GGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFV 385 HSP 3 Score: 89.3521 bits (220), Expect = 2.819e-20 Identity = 52/152 (34.21%), Postives = 89/152 (58.55%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVN 512
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+
Sbjct: 379 GGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLR 528
BLAST of Polyubiquitin vs. Ensembl Fly
Match: Ubi-p63E (gene:FBgn0003943 transcript:FBtr0333142) HSP 1 Score: 89.3521 bits (220), Expect = 3.505e-20 Identity = 52/161 (32.30%), Postives = 93/161 (57.76%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVNPSKNVFLYI 539
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+ + +++
Sbjct: 75 GGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFV 233 HSP 2 Score: 89.3521 bits (220), Expect = 3.505e-20 Identity = 52/161 (32.30%), Postives = 93/161 (57.76%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVNPSKNVFLYI 539
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+ + +++
Sbjct: 227 GGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFV 385 HSP 3 Score: 89.3521 bits (220), Expect = 3.505e-20 Identity = 52/161 (32.30%), Postives = 93/161 (57.76%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVNPSKNVFLYI 539
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+ + +++
Sbjct: 379 GGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFV 537 HSP 4 Score: 89.3521 bits (220), Expect = 3.505e-20 Identity = 52/161 (32.30%), Postives = 93/161 (57.76%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVNPSKNVFLYI 539
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+ + +++
Sbjct: 531 GGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFV 689 HSP 5 Score: 89.3521 bits (220), Expect = 3.783e-20 Identity = 52/152 (34.21%), Postives = 89/152 (58.55%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVN 512
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+
Sbjct: 607 GGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLR 756
BLAST of Polyubiquitin vs. Ensembl Fly
Match: Ubi-p63E (gene:FBgn0003943 transcript:FBtr0073178) HSP 1 Score: 89.3521 bits (220), Expect = 3.505e-20 Identity = 52/161 (32.30%), Postives = 93/161 (57.76%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVNPSKNVFLYI 539
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+ + +++
Sbjct: 75 GGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFV 233 HSP 2 Score: 89.3521 bits (220), Expect = 3.505e-20 Identity = 52/161 (32.30%), Postives = 93/161 (57.76%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVNPSKNVFLYI 539
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+ + +++
Sbjct: 227 GGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFV 385 HSP 3 Score: 89.3521 bits (220), Expect = 3.505e-20 Identity = 52/161 (32.30%), Postives = 93/161 (57.76%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVNPSKNVFLYI 539
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+ + +++
Sbjct: 379 GGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFV 537 HSP 4 Score: 89.3521 bits (220), Expect = 3.505e-20 Identity = 52/161 (32.30%), Postives = 93/161 (57.76%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVNPSKNVFLYI 539
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+ + +++
Sbjct: 531 GGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFV 689 HSP 5 Score: 89.3521 bits (220), Expect = 3.783e-20 Identity = 52/152 (34.21%), Postives = 89/152 (58.55%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVN 512
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+
Sbjct: 607 GGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLR 756
BLAST of Polyubiquitin vs. Ensembl Fly
Match: Ubi-p63E (gene:FBgn0003943 transcript:FBtr0073177) HSP 1 Score: 89.3521 bits (220), Expect = 3.505e-20 Identity = 52/161 (32.30%), Postives = 93/161 (57.76%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVNPSKNVFLYI 539
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+ + +++
Sbjct: 75 GGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFV 233 HSP 2 Score: 89.3521 bits (220), Expect = 3.505e-20 Identity = 52/161 (32.30%), Postives = 93/161 (57.76%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVNPSKNVFLYI 539
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+ + +++
Sbjct: 227 GGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFV 385 HSP 3 Score: 89.3521 bits (220), Expect = 3.505e-20 Identity = 52/161 (32.30%), Postives = 93/161 (57.76%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVNPSKNVFLYI 539
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+ + +++
Sbjct: 379 GGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFV 537 HSP 4 Score: 89.3521 bits (220), Expect = 3.505e-20 Identity = 52/161 (32.30%), Postives = 93/161 (57.76%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVNPSKNVFLYI 539
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+ + +++
Sbjct: 531 GGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFV 689 HSP 5 Score: 89.3521 bits (220), Expect = 3.783e-20 Identity = 52/152 (34.21%), Postives = 89/152 (58.55%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVN 512
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+
Sbjct: 607 GGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLR 756
BLAST of Polyubiquitin vs. Ensembl Zebrafish
Match: ubb (ubiquitin B [Source:ZFIN;Acc:ZDB-GENE-050411-10]) HSP 1 Score: 92.0485 bits (227), Expect = 2.851e-22 Identity = 57/166 (34.34%), Postives = 94/166 (56.63%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVNPSKNVFLYIFGAIF 554
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV L + GAIF
Sbjct: 75 GGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV--------LRLRGAIF 230
BLAST of Polyubiquitin vs. Ensembl Zebrafish
Match: CR848813.2 (pep chromosome:GRCz11:CHR_ALT_CTG5_1_42:42047434:42048357:1 gene:ENSDARG00000116968.1 transcript:ENSDART00000187237.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:CR848813.2) HSP 1 Score: 89.3521 bits (220), Expect = 8.974e-21 Identity = 52/161 (32.30%), Postives = 93/161 (57.76%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVNPSKNVFLYI 539
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+ + +++
Sbjct: 75 GGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFV 233 HSP 2 Score: 89.3521 bits (220), Expect = 9.735e-21 Identity = 52/152 (34.21%), Postives = 89/152 (58.55%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVN 512
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+
Sbjct: 151 GGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLR 300
BLAST of Polyubiquitin vs. Ensembl Zebrafish
Match: UBB (si:ch211-202a12.4 [Source:ZFIN;Acc:ZDB-GENE-030131-8486]) HSP 1 Score: 89.3521 bits (220), Expect = 3.069e-20 Identity = 52/161 (32.30%), Postives = 93/161 (57.76%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVNPSKNVFLYI 539
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+ + +++
Sbjct: 75 GGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFV 233 HSP 2 Score: 89.3521 bits (220), Expect = 3.069e-20 Identity = 52/161 (32.30%), Postives = 93/161 (57.76%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVNPSKNVFLYI 539
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+ + +++
Sbjct: 227 GGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFV 385 HSP 3 Score: 89.3521 bits (220), Expect = 3.285e-20 Identity = 52/152 (34.21%), Postives = 89/152 (58.55%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVN 512
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+
Sbjct: 303 GGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLR 452
BLAST of Polyubiquitin vs. Ensembl Zebrafish
Match: ubc (ubiquitin C [Source:ZFIN;Acc:ZDB-GENE-061110-88]) HSP 1 Score: 89.3521 bits (220), Expect = 3.597e-20 Identity = 52/161 (32.30%), Postives = 93/161 (57.76%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVNPSKNVFLYI 539
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+ + +++
Sbjct: 75 GGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFV 233 HSP 2 Score: 89.3521 bits (220), Expect = 3.597e-20 Identity = 52/161 (32.30%), Postives = 93/161 (57.76%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVNPSKNVFLYI 539
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+ + +++
Sbjct: 227 GGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFV 385 HSP 3 Score: 89.3521 bits (220), Expect = 3.846e-20 Identity = 52/152 (34.21%), Postives = 89/152 (58.55%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVN 512
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+
Sbjct: 379 GGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLR 528
BLAST of Polyubiquitin vs. Ensembl Zebrafish
Match: ubb (ubiquitin B [Source:ZFIN;Acc:ZDB-GENE-050411-10]) HSP 1 Score: 89.3521 bits (220), Expect = 4.373e-20 Identity = 52/161 (32.30%), Postives = 93/161 (57.76%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVNPSKNVFLYI 539
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+ + +++
Sbjct: 75 GGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFV 233 HSP 2 Score: 89.3521 bits (220), Expect = 4.373e-20 Identity = 52/161 (32.30%), Postives = 93/161 (57.76%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVNPSKNVFLYI 539
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+ + +++
Sbjct: 227 GGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFV 385 HSP 3 Score: 89.3521 bits (220), Expect = 4.373e-20 Identity = 52/161 (32.30%), Postives = 93/161 (57.76%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVNPSKNVFLYI 539
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+ + +++
Sbjct: 379 GGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFV 537 HSP 4 Score: 89.3521 bits (220), Expect = 4.675e-20 Identity = 52/152 (34.21%), Postives = 89/152 (58.55%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVN 512
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+
Sbjct: 455 GGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLR 604
BLAST of Polyubiquitin vs. Ensembl Xenopus
Match: ENSXETT00000033679.1 (ubiquitin C [Source:NCBI gene;Acc:448120]) HSP 1 Score: 89.3521 bits (220), Expect = 3.760e-21 Identity = 52/152 (34.21%), Postives = 89/152 (58.55%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVN 512
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+
Sbjct: 75 GGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLR 224
BLAST of Polyubiquitin vs. Ensembl Xenopus
Match: pdcd11 (programmed cell death 11 [Source:Xenbase;Acc:XB-GENE-967218]) HSP 1 Score: 89.3521 bits (220), Expect = 5.831e-20 Identity = 52/152 (34.21%), Postives = 89/152 (58.55%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVN 512
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+
Sbjct: 75 GGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLR 224 HSP 2 Score: 89.3521 bits (220), Expect = 5.831e-20 Identity = 52/152 (34.21%), Postives = 89/152 (58.55%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVN 512
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+
Sbjct: 227 GGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLR 376 HSP 3 Score: 88.1965 bits (217), Expect = 1.794e-19 Identity = 52/152 (34.21%), Postives = 89/152 (58.55%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVN 512
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K+KI + + I ++Q L+ LE+ RTL YN++ ++LV+
Sbjct: 499 GGMQIFVKTLTGKTITLEVEPSDTIENVKGKIQDKEGIPPDQQRLIFAGKQLEDGRTLCDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPS--DTIENVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLCDYNIQKESTLHLVLR 648 HSP 4 Score: 65.855 bits (159), Expect = 6.675e-12 Identity = 36/98 (36.73%), Postives = 58/98 (59.18%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIA 350
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE +
Sbjct: 575 GGMQIFVKTLTGKTITLEVEPSDTIENVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLCDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPS 672
BLAST of Polyubiquitin vs. Ensembl Xenopus
Match: pdcd11 (programmed cell death 11 [Source:Xenbase;Acc:XB-GENE-967218]) HSP 1 Score: 89.3521 bits (220), Expect = 6.154e-20 Identity = 52/152 (34.21%), Postives = 89/152 (58.55%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVN 512
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+
Sbjct: 86 GGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLR 235 HSP 2 Score: 89.3521 bits (220), Expect = 6.154e-20 Identity = 52/152 (34.21%), Postives = 89/152 (58.55%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVN 512
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+
Sbjct: 238 GGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLR 387 HSP 3 Score: 88.5817 bits (218), Expect = 1.294e-19 Identity = 52/152 (34.21%), Postives = 89/152 (58.55%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVN 512
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K+KI + + I ++Q L+ LE+ RTL YN++ ++LV+
Sbjct: 586 GGMQIFVKTLTGKTITLEVEPSDTIENVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLCDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPS--DTIENVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLCDYNIQKESTLHLVLR 735 HSP 4 Score: 87.8113 bits (216), Expect = 1.893e-19 Identity = 52/152 (34.21%), Postives = 89/152 (58.55%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVN 512
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K+KI + + I ++Q L+ LE+ RTL YN++ ++LV+
Sbjct: 510 GGMQIFVKTLTGKTITLEVEPSDTIENVKGKIQDKEGIPPDQQRLIFAGKQLEDGRTLCDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPS--DTIENVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLCDYNIQKESTLHLVLR 659
BLAST of Polyubiquitin vs. Ensembl Mouse
Match: Ubb (ubiquitin B [Source:MGI Symbol;Acc:MGI:98888]) HSP 1 Score: 89.3521 bits (220), Expect = 3.435e-21 Identity = 52/161 (32.30%), Postives = 93/161 (57.76%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVNPSKNVFLYI 539
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+ + +++
Sbjct: 75 GGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFV 233
BLAST of Polyubiquitin vs. Ensembl Mouse
Match: Ubb (ubiquitin B [Source:MGI Symbol;Acc:MGI:98888]) HSP 1 Score: 89.3521 bits (220), Expect = 8.959e-21 Identity = 52/161 (32.30%), Postives = 93/161 (57.76%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVNPSKNVFLYI 539
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+ + +++
Sbjct: 75 GGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFV 233 HSP 2 Score: 89.3521 bits (220), Expect = 9.144e-21 Identity = 52/152 (34.21%), Postives = 89/152 (58.55%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVN 512
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+
Sbjct: 151 GGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLR 300
BLAST of Polyubiquitin vs. Ensembl Mouse
Match: Ubc (ubiquitin C [Source:MGI Symbol;Acc:MGI:98889]) HSP 1 Score: 87.0409 bits (214), Expect = 1.321e-20 Identity = 51/150 (34.00%), Postives = 88/150 (58.67%), Query Frame = 3
Query: 72 MKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVN 512
M+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+
Sbjct: 1 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLR 148 HSP 2 Score: 72.4034 bits (176), Expect = 3.675e-15 Identity = 43/127 (33.86%), Postives = 73/127 (57.48%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNA 437
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+
Sbjct: 75 GGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPS--DTIENVKAKIQDKEGIPPDQQRLIFAG 199
BLAST of Polyubiquitin vs. Ensembl Mouse
Match: Ubc (ubiquitin C [Source:MGI Symbol;Acc:MGI:98889]) HSP 1 Score: 89.7373 bits (221), Expect = 4.070e-20 Identity = 52/152 (34.21%), Postives = 89/152 (58.55%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVN 512
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+
Sbjct: 75 GGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLR 224 HSP 2 Score: 89.7373 bits (221), Expect = 4.070e-20 Identity = 52/152 (34.21%), Postives = 89/152 (58.55%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVN 512
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+
Sbjct: 227 GGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLR 376 HSP 3 Score: 89.7373 bits (221), Expect = 4.070e-20 Identity = 52/152 (34.21%), Postives = 89/152 (58.55%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVN 512
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+
Sbjct: 379 GGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLR 528 HSP 4 Score: 89.7373 bits (221), Expect = 4.070e-20 Identity = 52/152 (34.21%), Postives = 89/152 (58.55%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVN 512
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+
Sbjct: 531 GGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLR 680
BLAST of Polyubiquitin vs. Ensembl Mouse
Match: Ubc (ubiquitin C [Source:MGI Symbol;Acc:MGI:98889]) HSP 1 Score: 89.7373 bits (221), Expect = 4.070e-20 Identity = 52/152 (34.21%), Postives = 89/152 (58.55%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVN 512
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+
Sbjct: 75 GGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLR 224 HSP 2 Score: 89.7373 bits (221), Expect = 4.070e-20 Identity = 52/152 (34.21%), Postives = 89/152 (58.55%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVN 512
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+
Sbjct: 227 GGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLR 376 HSP 3 Score: 89.7373 bits (221), Expect = 4.070e-20 Identity = 52/152 (34.21%), Postives = 89/152 (58.55%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVN 512
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+
Sbjct: 379 GGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLR 528 HSP 4 Score: 89.7373 bits (221), Expect = 4.070e-20 Identity = 52/152 (34.21%), Postives = 89/152 (58.55%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVN 512
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+
Sbjct: 531 GGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLR 680
BLAST of Polyubiquitin vs. UniProt/SwissProt
Match: sp|P62972|UBIQP_XENLA (Polyubiquitin (Fragment) OS=Xenopus laevis OX=8355 PE=1 SV=2) HSP 1 Score: 89.3521 bits (220), Expect = 5.747e-21 Identity = 52/152 (34.21%), Postives = 89/152 (58.55%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVN 512
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+
Sbjct: 14 GGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLR 163
BLAST of Polyubiquitin vs. UniProt/SwissProt
Match: sp|P0CG60|UBB_PONPY (Polyubiquitin-B OS=Pongo pygmaeus OX=9600 GN=UBB PE=3 SV=1) HSP 1 Score: 89.7373 bits (221), Expect = 1.651e-20 Identity = 52/152 (34.21%), Postives = 89/152 (58.55%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVN 512
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+
Sbjct: 75 GGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLR 224
BLAST of Polyubiquitin vs. UniProt/SwissProt
Match: sp|P0CG65|UBB_PANTR (Polyubiquitin-B OS=Pan troglodytes OX=9598 GN=UBB PE=3 SV=1) HSP 1 Score: 89.7373 bits (221), Expect = 1.651e-20 Identity = 52/152 (34.21%), Postives = 89/152 (58.55%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVN 512
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+
Sbjct: 75 GGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLR 224
BLAST of Polyubiquitin vs. UniProt/SwissProt
Match: sp|P0CG47|UBB_HUMAN (Polyubiquitin-B OS=Homo sapiens OX=9606 GN=UBB PE=1 SV=1) HSP 1 Score: 89.7373 bits (221), Expect = 1.651e-20 Identity = 52/152 (34.21%), Postives = 89/152 (58.55%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVN 512
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+
Sbjct: 75 GGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLR 224
BLAST of Polyubiquitin vs. UniProt/SwissProt
Match: sp|P0CG67|UBB_GORGO (Polyubiquitin-B OS=Gorilla gorilla gorilla OX=9595 GN=UBB PE=3 SV=1) HSP 1 Score: 89.7373 bits (221), Expect = 1.651e-20 Identity = 52/152 (34.21%), Postives = 89/152 (58.55%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVN 512
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+
Sbjct: 75 GGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLR 224
BLAST of Polyubiquitin vs. TrEMBL
Match: A0A5J5WVR6 (Uncharacterized protein OS=Gossypium barbadense OX=3634 GN=ES319_A01G076100v1 PE=4 SV=1) HSP 1 Score: 95.9005 bits (237), Expect = 6.506e-21 Identity = 55/175 (31.43%), Postives = 95/175 (54.29%), Query Frame = 3
Query: 33 YY*LTVFILTDSGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVL-IHDDIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVNPSKNVFLYIFGAIF 554
+ ++V + GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL + + + V ++ +E+ D + ++K KI + + I ++Q L+ LEN RTL YN+K N PS F+++ G F
Sbjct: 18 WCFVSVVVCKCGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLCGGMQIFVKTLTRKTITLEVESSDTIDNVKAKIQDKESIPPDQQRLIFAGKQLENGRTLADYNIKRN-------RPSIWFFVFVVGCKF 185
BLAST of Polyubiquitin vs. TrEMBL
Match: A0A5D2RQH5 (Uncharacterized protein OS=Gossypium tomentosum OX=34277 GN=ES332_A01G089600v1 PE=4 SV=1) HSP 1 Score: 95.1301 bits (235), Expect = 1.322e-20 Identity = 55/175 (31.43%), Postives = 95/175 (54.29%), Query Frame = 3
Query: 33 YY*LTVFILTDSGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVL-IHDDIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVNPSKNVFLYIFGAIF 554
+ ++V + GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL + + + V ++ +E+ D + ++K KI + + I ++Q L+ LEN RTL YN+K N PS F+++ G F
Sbjct: 18 WCFVSVVVCKCGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLCGGMQIFVKTLTRKTITLEVESSDTIDNVKAKIQDKEGIPQDQQRLIFAGKQLENGRTLADYNIKRN-------RPSIWFFVFVVGCKF 185
BLAST of Polyubiquitin vs. TrEMBL
Match: A0A5D3ACT7 (Uncharacterized protein OS=Gossypium mustelinum OX=34275 GN=E1A91_A01G078100v1 PE=4 SV=1) HSP 1 Score: 95.1301 bits (235), Expect = 1.351e-20 Identity = 55/175 (31.43%), Postives = 95/175 (54.29%), Query Frame = 3
Query: 33 YY*LTVFILTDSGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVL-IHDDIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVNPSKNVFLYIFGAIF 554
+ + V + GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL + + + V ++ +E+ D + ++K KI + + I ++Q L+ + LEN RTL YN+K N PS F+++ G F
Sbjct: 18 WCFVPVVVCKCGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLCGGMQIFVKTLTRKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFASKQLENGRTLADYNIKRN-------RPSIWFFVFVVGCKF 185
BLAST of Polyubiquitin vs. TrEMBL
Match: A0A5D2HJS0 (Uncharacterized protein OS=Gossypium darwinii OX=34276 GN=ES288_A01G083600v1 PE=4 SV=1) HSP 1 Score: 94.7449 bits (234), Expect = 2.268e-20 Identity = 54/175 (30.86%), Postives = 95/175 (54.29%), Query Frame = 3
Query: 33 YY*LTVFILTDSGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVL-IHDDIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVNPSKNVFLYIFGAIF 554
+ +++ + GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL + + + V ++ +E+ D + ++K KI + + I ++Q L+ LEN RTL YN+K N PS F+++ G F
Sbjct: 18 WCFVSLVVCKCGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLCGGMQIFVKTLTRKTITLEVESSDTIDNVKAKIQDKESIPPDQQRLIFAGKQLENGRTLADYNIKRN-------RPSIWFFVFVVGCKF 185
BLAST of Polyubiquitin vs. TrEMBL
Match: A0A067QVQ6 (Ubiquitin OS=Zootermopsis nevadensis OX=136037 GN=L798_01850 PE=4 SV=1) HSP 1 Score: 93.2041 bits (230), Expect = 1.901e-19 Identity = 54/152 (35.53%), Postives = 90/152 (59.21%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLI---HDDIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVN 512
GM+IFVK L GK +T E +T+ ++K+K+ +K G P++Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE A D + +LK K+ + + I E+Q L+ LE+ RTL+ YN++ ++LV+
Sbjct: 75 GGMQIFVKTLTGKTITLEVEPADTIENLKKKLQDKEGIPPEQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPA--DTIENLKKKLQDKEGIPPEQQRLMFAGKQLEDGRTLSDYNIQKGSTLHLVLR 224
BLAST of Polyubiquitin vs. Ensembl Cavefish
Match: si:ch211-202a12.4 (ubiquitin B [Source:HGNC Symbol;Acc:HGNC:12463]) HSP 1 Score: 89.7373 bits (221), Expect = 1.324e-20 Identity = 52/152 (34.21%), Postives = 89/152 (58.55%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVN 512
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+
Sbjct: 82 GGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLR 231
BLAST of Polyubiquitin vs. Ensembl Sea Lamprey
Match: UBB (ubiquitin B [Source:HGNC Symbol;Acc:HGNC:12463]) HSP 1 Score: 89.3521 bits (220), Expect = 5.313e-21 Identity = 52/161 (32.30%), Postives = 93/161 (57.76%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVNPSKNVFLYI 539
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+ + +++
Sbjct: 88 GGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFV 246 HSP 2 Score: 89.3521 bits (220), Expect = 5.313e-21 Identity = 52/161 (32.30%), Postives = 93/161 (57.76%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVNPSKNVFLYI 539
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+ + +++
Sbjct: 240 GGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFV 398
BLAST of Polyubiquitin vs. Ensembl Sea Lamprey
Match: ENSPMAT00000006254.1 (pep scaffold:Pmarinus_7.0:GL477433:3744:20801:-1 gene:ENSPMAG00000005642.1 transcript:ENSPMAT00000006254.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 83.1889 bits (204), Expect = 6.217e-19 Identity = 48/148 (32.43%), Postives = 82/148 (55.41%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHDDIAVHVLIG--ENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINL 503
G ++ VK+LEGK L L+ETVL++K ++ K P +Q+L + G+ L D L G+ + ST+N L+H D AV V + N ++ +E+ + V+ LK K+++ I V +Q L + LE+ + L SY ++ + I +
Sbjct: 226 GGYQVVVKDLEGKSLRLMVKLSETVLELKARIQSKWKVSPAQQRLAYGGTALQDGTTLAKCGLKQNSTVN--LLHVDNAVDVFVKTLANKTLTIEVKLSNSVLSLKQKVHQKAGIAVNQQILTFGSHTLEDDQKLESYGIQQHSTITM 371 HSP 2 Score: 65.4698 bits (158), Expect = 9.331e-13 Identity = 48/161 (29.81%), Postives = 85/161 (52.80%), Query Frame = 3
Query: 63 DSGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNI---VLIHDDIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLV-VNPSKNVFL 533
D+ + +FVK L K LT E L+ +VL +K+KVH+K G +Q L F L D L+ GI + ST+ + + + V L G+++ + V+++ + V++LK +I + +Q L L++ TL +K N +NL+ V+ + +VF+
Sbjct: 149 DNAVDVFVKTLANKTLTIEVKLSNSVLSLKQKVHQKAGIAVNQQILTFGSHTLEDDQKLESYGIQQHSTITMQGRLRGGYQVVVKDLEGKSLRLMVKLS--ETVLELKARIQSKWKVSPAQQRLAYGGTALQDGTTLAKCGLKQNSTVNLLHVDNAVDVFV 307 HSP 3 Score: 60.8474 bits (146), Expect = 2.766e-11 Identity = 43/158 (27.22%), Postives = 81/158 (51.27%), Query Frame = 3
Query: 72 MKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNI---VLIHDDIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLV-VNPSKNVFL 533
++IFV N +G T++ +L++ V +K K+H + G P++Q L F L L+ I QST+ + + I V L G ++ + V+++ + V++LK +I + +Q L L++ TL +K N +NL+ V+ + +VF+
Sbjct: 1 VQIFVVNFQGSTRTYDVSLSDRVQQLKVKIHREEGVPPREQILTFESQQLEGERTLRSYNIRAQSTIQLQARLRGGQSIVVKDLEGNSLRLMVKLS--ESVLELKARIQSKWKVSPAQQRLAYGGTALQDGTTLAKCGLKQNSTVNLLHVDNAVDVFV 156 HSP 4 Score: 50.8322 bits (120), Expect = 6.325e-8 Identity = 28/72 (38.89%), Postives = 41/72 (56.94%), Query Frame = 3
Query: 63 DSGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNI 278
D+ + +FVK L K LT E L+ +VL +K+KVH+K G +Q L F L D L+ GI + ST+ +
Sbjct: 300 DNAVDVFVKTLANKTLTIEVKLSNSVLSLKQKVHQKAGIAVNQQILTFGSHTLEDDQKLESYGIQQHSTITM 371
BLAST of Polyubiquitin vs. Ensembl Sea Lamprey
Match: ENSPMAT00000006261.1 (pep scaffold:Pmarinus_7.0:GL477433:28222:29567:1 gene:ENSPMAG00000005664.1 transcript:ENSPMAT00000006261.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 51.2174 bits (121), Expect = 1.092e-8 Identity = 28/72 (38.89%), Postives = 41/72 (56.94%), Query Frame = 3
Query: 63 DSGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNI 278
D+ + +FVK L K LT E L+ +VL +K+KVH+K G +Q L F L D L+ GI + ST+ +
Sbjct: 73 DNAVDVFVKTLANKTLTIEVKLSNSVLSLKQKVHQKAGIAVNQQILTFGSHTLEDDQKLESYGIQQHSTITM 144
BLAST of Polyubiquitin vs. Ensembl Yeast
Match: UBI4 (Ubiquitin; becomes conjugated to proteins, marking them for selective degradation via the ubiquitin-26S proteasome system; essential for the cellular stress response; encoded as a polyubiquitin precursor comprised of 5 head-to-tail repeats; protein abundance increases in response to DNA replication stress [Source:SGD;Acc:S000003962]) HSP 1 Score: 88.9669 bits (219), Expect = 2.819e-21 Identity = 52/161 (32.30%), Postives = 94/161 (58.39%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVNPSKNVFLYI 539
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K+KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+ + +++
Sbjct: 75 GGMQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESS--DTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFV 233 HSP 2 Score: 88.5817 bits (218), Expect = 3.650e-21 Identity = 52/152 (34.21%), Postives = 90/152 (59.21%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVN 512
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K+KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+
Sbjct: 227 GGMQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESS--DTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLR 376
BLAST of Polyubiquitin vs. Ensembl Nematostella
Match: EDO32403 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SV54]) HSP 1 Score: 88.1965 bits (217), Expect = 2.173e-21 Identity = 51/152 (33.55%), Postives = 88/152 (57.89%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVN 512
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q + LE+ RTL+ YN++ ++LV+
Sbjct: 75 GGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPS--DTIENVKAKIQDKEGIPPDQQRFIFAGKQLEDGRTLSDYNIQKESTLHLVLR 224
BLAST of Polyubiquitin vs. Ensembl Nematostella
Match: EDO32547 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SUP6]) HSP 1 Score: 89.3521 bits (220), Expect = 1.168e-20 Identity = 52/161 (32.30%), Postives = 93/161 (57.76%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVNPSKNVFLYI 539
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+ + +++
Sbjct: 75 GGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFV 233 HSP 2 Score: 89.3521 bits (220), Expect = 1.168e-20 Identity = 52/161 (32.30%), Postives = 93/161 (57.76%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVNPSKNVFLYI 539
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+ + +++
Sbjct: 227 GGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFV 385 HSP 3 Score: 89.3521 bits (220), Expect = 1.249e-20 Identity = 52/152 (34.21%), Postives = 89/152 (58.55%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVN 512
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+
Sbjct: 379 GGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLR 528
BLAST of Polyubiquitin vs. Ensembl Medaka
Match: ubb (ubiquitin C [Source:NCBI gene;Acc:101172100]) HSP 1 Score: 89.3521 bits (220), Expect = 3.798e-20 Identity = 52/161 (32.30%), Postives = 93/161 (57.76%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVNPSKNVFLYI 539
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+ + +++
Sbjct: 75 GGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFV 233 HSP 2 Score: 89.3521 bits (220), Expect = 3.798e-20 Identity = 52/161 (32.30%), Postives = 93/161 (57.76%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVNPSKNVFLYI 539
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+ + +++
Sbjct: 227 GGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFV 385 HSP 3 Score: 89.3521 bits (220), Expect = 3.908e-20 Identity = 52/152 (34.21%), Postives = 89/152 (58.55%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVN 512
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+
Sbjct: 379 GGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLR 528 HSP 4 Score: 89.3521 bits (220), Expect = 3.908e-20 Identity = 52/152 (34.21%), Postives = 89/152 (58.55%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHD---DIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVN 512
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+
Sbjct: 628 GGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLR 777 HSP 5 Score: 84.3445 bits (207), Expect = 2.126e-18 Identity = 54/182 (29.67%), Postives = 96/182 (52.75%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVL-------IHDD-----------------IAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVNPSKNVFLYI 539
GM+IFVK L GK +T E ++T+ ++K K+ +K G P +Q+L+F G L D L D I ++ST+++VL I D+ I V L G+ +++ VE + D + ++K KI + + I ++Q L+ LE+ RTL+ YN++ ++LV+ + +++
Sbjct: 455 GGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGVVYIRDNKYQILKAKSFFTGVNMQIFVKTLTGKTITLEVEPS--DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFV 634
BLAST of Polyubiquitin vs. Planmine SMEST
Match: SMESG000079959.1 (SMESG000079959.1) HSP 1 Score: 340.887 bits (873), Expect = 9.650e-121 Identity = 175/175 (100.00%), Postives = 175/175 (100.00%), Query Frame = 3
Query: 72 MKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHDDIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVNPSKNVFLYIFGAIFAVILALEVAKFFYN 596
MKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHDDIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVNPSKNVFLYIFGAIFAVILALEVAKFFYN
Sbjct: 1 MKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHDDIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVNPSKNVFLYIFGAIFAVILALEVAKFFYN 175
BLAST of Polyubiquitin vs. Planmine SMEST
Match: SMESG000048258.1 (SMESG000048258.1) HSP 1 Score: 102.449 bits (254), Expect = 9.566e-25 Identity = 50/157 (31.85%), Postives = 88/157 (56.05%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNI-VLIHDDIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVNPSKNVFL 533
GM+IF K ++GK + FE E + D+K K+ EK G P +Q+LV+ G +L D ++D GI E+S +N+ + + + + +V +E+ K +++ D+K KI E + +EQ L+ ILE++RT+ Y + + +IN+V P +
Sbjct: 834 GGMQIFAKTMDGKTVAFEVRKAEKIRDVKLKIQEKIGLAPGEQRLVYQGGILEDERTVEDYGIAEKSVINVPARLRGGMQIFAKTMDGKTVAIEVQKTEKIRDIKLKIQEKIGLAPDEQILVYQGGILEDERTVEEYQIAEKSVINVVARPRSQLIW 990 HSP 2 Score: 98.5969 bits (244), Expect = 2.330e-23 Identity = 56/174 (32.18%), Postives = 95/174 (54.60%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHDDIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVIL-ENKRTLNSYNVKDNDIINLVVNPSKNVFLYIFGAIFAVILALEVAK 584
GM+IF K ++GK + FE E + D+K K+ EK G P +Q+LV+ G +L D ++D GI E+S + + + I + G+ V+ E+ K D + D+K KI E + +EQ L+ IL E++RT+ Y + + +IN+V P + ++ + I+ +EV K
Sbjct: 463 GGMQIFAKTMDGKTVAFEVRKAEKIRDVKLKIQEKIGLAPGEQRLVYQGGILEDERTVEDYGIAEKSVITVAIGGMVILAKTMDGKTVA--FEVQKTDTIRDVKLKIQEKVGVAPDEQILVYQGGILYEDERTVEEYQIAEKSVINVVARPRGGMQIFAI-TVDCKIVPIEVQK 633 HSP 3 Score: 85.8853 bits (211), Expect = 5.134e-19 Identity = 46/148 (31.08%), Postives = 81/148 (54.73%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHDDIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVV 509
GM+IF ++ K++ E +T+ D+K K+ EK G P +Q LV+ G +L D +D GI ++S +++V I D+ + G+ V +E+ K D + D+K +I + +E L+ ILE++RT Y + D +I++V
Sbjct: 238 GGMQIFAITVDCKIVPIEVQKTDTIRDVKLKIQEKVGVAPDEQILVYQGGILEDERTAEDYGIADESMIDVVAIR-DMQIFPKTGDGKIVPIEVQKTDTIRDVKLRIQNQIGLAPDEPKLVYQGGILEDERTAEDYGIADESMIDVVA 384 HSP 4 Score: 84.7297 bits (208), Expect = 1.196e-18 Identity = 50/164 (30.49%), Postives = 87/164 (53.05%), Query Frame = 3
Query: 45 TVFILTDSGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLH-DHDVLKDVGIVEQSTMNIVL-IHDDIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVNPSKNVF 530
+V + GM I K ++GK + FE +T+ D+K K+ EK G P +Q LV+ G +L+ D +++ I E+S +N+V + + + + V +E+ K D + D+K KI E + +EQ L+ ILE++RT Y + D +I++V +F
Sbjct: 154 SVITVAIGGMVILAKTMDGKTVAFEVQKTDTIRDVKLKIQEKVGVAPDEQILVYQGGILYEDERTVEEYQIAEKSVINVVARPRGGMQIFAITVDCKIVPIEVQKTDTIRDVKLKIQEKVGVAPDEQILVYQGGILEDERTAEDYGIADESMIDVVAIRDMQIF 317 HSP 5 Score: 83.5741 bits (205), Expect = 2.951e-18 Identity = 46/156 (29.49%), Postives = 86/156 (55.13%), Query Frame = 3
Query: 72 MKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHDDIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVIL-ENKRTLNSYNVKDNDIINLVVNPSKNVFLY 536
M+IF K +GK++ E +T+ D+K ++ + G P + KLV+ G +L D +D GI ++S +++V I + + + + +V E+ K D + D+K KI E + +EQ L+ IL E++RT+ Y + + +IN+V P + ++
Sbjct: 314 MQIFPKTGDGKIVPIEVQKTDTIRDVKLRIQNQIGLAPDEPKLVYQGGILEDERTAEDYGIADESMIDVVAIGGMVILAKTM-DGKTVAFEVQKTDTIRDVKLKIQEKVGVAPDEQILVYQGGILYEDERTVEEYQIAEKSVINVVARPRGGMQIF 468 HSP 6 Score: 82.0333 bits (201), Expect = 1.053e-17 Identity = 48/154 (31.17%), Postives = 83/154 (53.90%), Query Frame = 3
Query: 45 TVFILTDSGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLH-DHDVLKDVGIVEQSTMNIVL-IHDDIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIIN 500
+V + GM I K ++GK + FE +T+ D+K K+ EK G P +Q LV+ G +L+ D +++ I E+S +N+V + + + + V +E+ K D + D+K KI E + +EQ L+ ILE++RT Y + D +I+
Sbjct: 529 SVITVAIGGMVILAKTMDGKTVAFEVQKTDTIRDVKLKIQEKVGVAPDEQILVYQGGILYEDERTVEEYQIAEKSVINVVARPRGGMQIFAITVDCKIVPIEVQKTDTIRDVKLKIQEKVGVAPDEQILVYQGGILEDERTAEDYGIADESMID 682 HSP 7 Score: 81.2629 bits (199), Expect = 2.085e-17 Identity = 47/154 (30.52%), Postives = 84/154 (54.55%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLH-DHDVLKDVGIVEQSTMNIVL-IHDDIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVNPSK 521
GM I K ++GK + FE +T+ D+K K+ EK G P +Q LV+ G +L+ D +++ I E+S +N+V + + + +V E+ K +++ D+K KI E + EQ L+ ILE++RT+ Y + + +IN+ P++
Sbjct: 757 GGMVILAKTMDGKTVAFEVQKTDTIRDVKLKIQEKVGVAPDEQILVYQGGILYEDERTVEEYQIAEKSVINVVARPRGGMQIFAKTMDGKTVAFEVRKAEKIRDVKLKIQEKIGLAPGEQRLVYQGGILEDERTVEDYGIAEKSVINV---PAR 907 HSP 8 Score: 80.4925 bits (197), Expect = 3.900e-17 Identity = 45/151 (29.80%), Postives = 81/151 (53.64%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLH-DHDVLKDVGIVEQSTMNIVL-IHDDIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVN 512
GM I K ++GK + FE +T+ D+K K+ EK G P +Q LV+ G +L+ D +++ I E+S +N+V + + + +V E+ K +++ D+K KI E + EQ L+ ILE++RT+ Y + + +I + +
Sbjct: 386 GGMVILAKTMDGKTVAFEVQKTDTIRDVKLKIQEKVGVAPDEQILVYQGGILYEDERTVEEYQIAEKSVINVVARPRGGMQIFAKTMDGKTVAFEVRKAEKIRDVKLKIQEKIGLAPGEQRLVYQGGILEDERTVEDYGIAEKSVITVAIG 536 HSP 9 Score: 78.9518 bits (193), Expect = 1.205e-16 Identity = 45/161 (27.95%), Postives = 86/161 (53.42%), Query Frame = 3
Query: 57 LTDSGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHDDIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVIL-ENKRTLNSYNVKDNDIINLVVNPSKNVFLY 536
+ D G +N +GK++ E +T+ D+K ++ + G P + KLV+ G +L D +D GI ++S +++V I + + + + +V E+ K D + D+K KI E + +EQ L+ IL E++RT+ Y + + +IN+V P + ++
Sbjct: 680 MIDKGHANISQNGDGKIVPIEVQKTDTIRDVKLRIQNQIGLAPDEPKLVYQGGILEDERTAEDYGIADESMIDVVAIGGMVILAKTM-DGKTVAFEVQKTDTIRDVKLKIQEKVGVAPDEQILVYQGGILYEDERTVEEYQIAEKSVINVVARPRGGMQIF 839 HSP 10 Score: 77.411 bits (189), Expect = 3.862e-16 Identity = 47/173 (27.17%), Postives = 90/173 (52.02%), Query Frame = 3
Query: 69 GMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHDDIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVIL-ENKRTLNSYNVKDNDIINLVVNPSKNVFLYIFGAIFAVILALEVAK 584
GM+++++ + G + + D+K ++ + G P +QKLV+ G +L D ++D GI E+S + + + I + G+ V+ E+ K D + D+K KI E + +EQ L+ IL E++RT+ Y + + +IN+V P + ++ + I+ +EV K
Sbjct: 89 GMQMYLRRVGGTTELVTVGKADKIRDVKLRIQNQIGLAPDEQKLVYQGGILEDERTVEDYGIAEKSVITVAIGGMVILAKTMDGKTVA--FEVQKTDTIRDVKLKIQEKVGVAPDEQILVYQGGILYEDERTVEEYQIAEKSVINVVARPRGGMQIFAI-TVDCKIVPIEVQK 258 HSP 11 Score: 76.6406 bits (187), Expect = 7.780e-16 Identity = 45/148 (30.41%), Postives = 80/148 (54.05%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHDDIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVV 509
GM+IF ++ K++ E +T+ D+K K+ EK G P +Q LV+ G +L D +D GI ++S ++ H +I+ + G+ V +E+ K D + D+K +I + +E L+ ILE++RT Y + D +I++V
Sbjct: 613 GGMQIFAITVDCKIVPIEVQKTDTIRDVKLKIQEKVGVAPDEQILVYQGGILEDERTAEDYGIADESMID--KGHANISQN---GDGKIVPIEVQKTDTIRDVKLRIQNQIGLAPDEPKLVYQGGILEDERTAEDYGIADESMIDVVA 755 HSP 12 Score: 73.1738 bits (178), Expect = 1.076e-14 Identity = 45/158 (28.48%), Postives = 81/158 (51.27%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHDDIAVHV---LIGENV----SVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVNPS 518
GM+IF K ++GK + E E + DIK K+ EK G P +Q LV+ G +L D +++ I E+S +N+V + + L+G+N+ + ++ D++ +K K E+ I L IL++ R++ Y +++ DI+ +PS
Sbjct: 910 GGMQIFAKTMDGKTVAIEVQKTEKIRDIKLKIQEKIGLAPDEQILVYQGGILEDERTVEEYQIAEKSVINVVARPRSQLIWLQMKLVGKNIHGLNMMTLDAKAEDKITHIKKKFKEITGIASVIYQLRCRDQILKDGRSVGDYRLRNGDILTADFSPS 1067 HSP 13 Score: 57.7658 bits (138), Expect = 1.641e-9 Identity = 38/149 (25.50%), Postives = 75/149 (50.34%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHDDIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVN 512
GM+IFVK + +F + ++ + +K K+ + G + Q L +L D L+ GIV++S +NI L + +++ + V + K D++ D+K +I + +EQ L+ ILE++RT+ Y + + +I + +
Sbjct: 16 GGMRIFVKAWNENIFSFPAESDDKIYHLKSKIEKLLGIKMGNQILSNENEILEDEKTLEQYGIVDKSLINIEL---GMQMYLRRVGGTTELVTVGKADKIRDVKLRIQNQIGLAPDEQKLVYQGGILEDERTVEDYGIAEKSVITVAIG 161
BLAST of Polyubiquitin vs. Planmine SMEST
Match: SMESG000048258.1 (SMESG000048258.1) HSP 1 Score: 102.064 bits (253), Expect = 1.123e-24 Identity = 50/157 (31.85%), Postives = 88/157 (56.05%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNI-VLIHDDIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVNPSKNVFL 533
GM+IF K ++GK + FE E + D+K K+ EK G P +Q+LV+ G +L D ++D GI E+S +N+ + + + + +V +E+ K +++ D+K KI E + +EQ L+ ILE++RT+ Y + + +IN+V P +
Sbjct: 817 GGMQIFAKTMDGKTVAFEVRKAEKIRDVKLKIQEKIGLAPGEQRLVYQGGILEDERTVEDYGIAEKSVINVPARLRGGMQIFAKTMDGKTVAIEVQKTEKIRDIKLKIQEKIGLAPDEQILVYQGGILEDERTVEEYQIAEKSVINVVARPRSQLIW 973 HSP 2 Score: 98.2117 bits (243), Expect = 2.787e-23 Identity = 56/174 (32.18%), Postives = 95/174 (54.60%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHDDIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVIL-ENKRTLNSYNVKDNDIINLVVNPSKNVFLYIFGAIFAVILALEVAK 584
GM+IF K ++GK + FE E + D+K K+ EK G P +Q+LV+ G +L D ++D GI E+S + + + I + G+ V+ E+ K D + D+K KI E + +EQ L+ IL E++RT+ Y + + +IN+V P + ++ + I+ +EV K
Sbjct: 446 GGMQIFAKTMDGKTVAFEVRKAEKIRDVKLKIQEKIGLAPGEQRLVYQGGILEDERTVEDYGIAEKSVITVAIGGMVILAKTMDGKTVA--FEVQKTDTIRDVKLKIQEKVGVAPDEQILVYQGGILYEDERTVEEYQIAEKSVINVVARPRGGMQIFAI-TVDCKIVPIEVQK 616 HSP 3 Score: 85.5001 bits (210), Expect = 5.963e-19 Identity = 46/148 (31.08%), Postives = 81/148 (54.73%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHDDIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVV 509
GM+IF ++ K++ E +T+ D+K K+ EK G P +Q LV+ G +L D +D GI ++S +++V I D+ + G+ V +E+ K D + D+K +I + +E L+ ILE++RT Y + D +I++V
Sbjct: 221 GGMQIFAITVDCKIVPIEVQKTDTIRDVKLKIQEKVGVAPDEQILVYQGGILEDERTAEDYGIADESMIDVVAIR-DMQIFPKTGDGKIVPIEVQKTDTIRDVKLRIQNQIGLAPDEPKLVYQGGILEDERTAEDYGIADESMIDVVA 367 HSP 4 Score: 84.3445 bits (207), Expect = 1.457e-18 Identity = 50/164 (30.49%), Postives = 87/164 (53.05%), Query Frame = 3
Query: 45 TVFILTDSGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLH-DHDVLKDVGIVEQSTMNIVL-IHDDIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVNPSKNVF 530
+V + GM I K ++GK + FE +T+ D+K K+ EK G P +Q LV+ G +L+ D +++ I E+S +N+V + + + + V +E+ K D + D+K KI E + +EQ L+ ILE++RT Y + D +I++V +F
Sbjct: 137 SVITVAIGGMVILAKTMDGKTVAFEVQKTDTIRDVKLKIQEKVGVAPDEQILVYQGGILYEDERTVEEYQIAEKSVINVVARPRGGMQIFAITVDCKIVPIEVQKTDTIRDVKLKIQEKVGVAPDEQILVYQGGILEDERTAEDYGIADESMIDVVAIRDMQIF 300 HSP 5 Score: 83.1889 bits (204), Expect = 3.493e-18 Identity = 46/156 (29.49%), Postives = 86/156 (55.13%), Query Frame = 3
Query: 72 MKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHDDIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVIL-ENKRTLNSYNVKDNDIINLVVNPSKNVFLY 536
M+IF K +GK++ E +T+ D+K ++ + G P + KLV+ G +L D +D GI ++S +++V I + + + + +V E+ K D + D+K KI E + +EQ L+ IL E++RT+ Y + + +IN+V P + ++
Sbjct: 297 MQIFPKTGDGKIVPIEVQKTDTIRDVKLRIQNQIGLAPDEPKLVYQGGILEDERTAEDYGIADESMIDVVAIGGMVILAKTM-DGKTVAFEVQKTDTIRDVKLKIQEKVGVAPDEQILVYQGGILYEDERTVEEYQIAEKSVINVVARPRGGMQIF 451 HSP 6 Score: 81.6481 bits (200), Expect = 1.258e-17 Identity = 48/154 (31.17%), Postives = 83/154 (53.90%), Query Frame = 3
Query: 45 TVFILTDSGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLH-DHDVLKDVGIVEQSTMNIVL-IHDDIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIIN 500
+V + GM I K ++GK + FE +T+ D+K K+ EK G P +Q LV+ G +L+ D +++ I E+S +N+V + + + + V +E+ K D + D+K KI E + +EQ L+ ILE++RT Y + D +I+
Sbjct: 512 SVITVAIGGMVILAKTMDGKTVAFEVQKTDTIRDVKLKIQEKVGVAPDEQILVYQGGILYEDERTVEEYQIAEKSVINVVARPRGGMQIFAITVDCKIVPIEVQKTDTIRDVKLKIQEKVGVAPDEQILVYQGGILEDERTAEDYGIADESMID 665 HSP 7 Score: 80.8777 bits (198), Expect = 2.613e-17 Identity = 47/154 (30.52%), Postives = 84/154 (54.55%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLH-DHDVLKDVGIVEQSTMNIVL-IHDDIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVNPSK 521
GM I K ++GK + FE +T+ D+K K+ EK G P +Q LV+ G +L+ D +++ I E+S +N+V + + + +V E+ K +++ D+K KI E + EQ L+ ILE++RT+ Y + + +IN+ P++
Sbjct: 740 GGMVILAKTMDGKTVAFEVQKTDTIRDVKLKIQEKVGVAPDEQILVYQGGILYEDERTVEEYQIAEKSVINVVARPRGGMQIFAKTMDGKTVAFEVRKAEKIRDVKLKIQEKIGLAPGEQRLVYQGGILEDERTVEDYGIAEKSVINV---PAR 890 HSP 8 Score: 80.1073 bits (196), Expect = 4.885e-17 Identity = 45/151 (29.80%), Postives = 81/151 (53.64%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLH-DHDVLKDVGIVEQSTMNIVL-IHDDIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVN 512
GM I K ++GK + FE +T+ D+K K+ EK G P +Q LV+ G +L+ D +++ I E+S +N+V + + + +V E+ K +++ D+K KI E + EQ L+ ILE++RT+ Y + + +I + +
Sbjct: 369 GGMVILAKTMDGKTVAFEVQKTDTIRDVKLKIQEKVGVAPDEQILVYQGGILYEDERTVEEYQIAEKSVINVVARPRGGMQIFAKTMDGKTVAFEVRKAEKIRDVKLKIQEKIGLAPGEQRLVYQGGILEDERTVEDYGIAEKSVITVAIG 519 HSP 9 Score: 78.5666 bits (192), Expect = 1.439e-16 Identity = 45/161 (27.95%), Postives = 86/161 (53.42%), Query Frame = 3
Query: 57 LTDSGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHDDIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVIL-ENKRTLNSYNVKDNDIINLVVNPSKNVFLY 536
+ D G +N +GK++ E +T+ D+K ++ + G P + KLV+ G +L D +D GI ++S +++V I + + + + +V E+ K D + D+K KI E + +EQ L+ IL E++RT+ Y + + +IN+V P + ++
Sbjct: 663 MIDKGHANISQNGDGKIVPIEVQKTDTIRDVKLRIQNQIGLAPDEPKLVYQGGILEDERTAEDYGIADESMIDVVAIGGMVILAKTM-DGKTVAFEVQKTDTIRDVKLKIQEKVGVAPDEQILVYQGGILYEDERTVEEYQIAEKSVINVVARPRGGMQIF 822 HSP 10 Score: 77.0258 bits (188), Expect = 4.836e-16 Identity = 47/173 (27.17%), Postives = 90/173 (52.02%), Query Frame = 3
Query: 69 GMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHDDIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVIL-ENKRTLNSYNVKDNDIINLVVNPSKNVFLYIFGAIFAVILALEVAK 584
GM+++++ + G + + D+K ++ + G P +QKLV+ G +L D ++D GI E+S + + + I + G+ V+ E+ K D + D+K KI E + +EQ L+ IL E++RT+ Y + + +IN+V P + ++ + I+ +EV K
Sbjct: 72 GMQMYLRRVGGTTELVTVGKADKIRDVKLRIQNQIGLAPDEQKLVYQGGILEDERTVEDYGIAEKSVITVAIGGMVILAKTMDGKTVA--FEVQKTDTIRDVKLKIQEKVGVAPDEQILVYQGGILYEDERTVEEYQIAEKSVINVVARPRGGMQIFAI-TVDCKIVPIEVQK 241 HSP 11 Score: 76.2554 bits (186), Expect = 9.379e-16 Identity = 45/148 (30.41%), Postives = 80/148 (54.05%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHDDIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVV 509
GM+IF ++ K++ E +T+ D+K K+ EK G P +Q LV+ G +L D +D GI ++S ++ H +I+ + G+ V +E+ K D + D+K +I + +E L+ ILE++RT Y + D +I++V
Sbjct: 596 GGMQIFAITVDCKIVPIEVQKTDTIRDVKLKIQEKVGVAPDEQILVYQGGILEDERTAEDYGIADESMID--KGHANISQN---GDGKIVPIEVQKTDTIRDVKLRIQNQIGLAPDEPKLVYQGGILEDERTAEDYGIADESMIDVVA 738 HSP 12 Score: 72.7886 bits (177), Expect = 1.322e-14 Identity = 45/158 (28.48%), Postives = 81/158 (51.27%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHDDIAVHV---LIGENV----SVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVNPS 518
GM+IF K ++GK + E E + DIK K+ EK G P +Q LV+ G +L D +++ I E+S +N+V + + L+G+N+ + ++ D++ +K K E+ I L IL++ R++ Y +++ DI+ +PS
Sbjct: 893 GGMQIFAKTMDGKTVAIEVQKTEKIRDIKLKIQEKIGLAPDEQILVYQGGILEDERTVEEYQIAEKSVINVVARPRSQLIWLQMKLVGKNIHGLNMMTLDAKAEDKITHIKKKFKEITGIASVIYQLRCRDQILKDGRSVGDYRLRNGDILTADFSPS 1050
BLAST of Polyubiquitin vs. Planmine SMEST
Match: SMESG000048258.1 (SMESG000048258.1) HSP 1 Score: 102.064 bits (253), Expect = 1.248e-24 Identity = 50/157 (31.85%), Postives = 88/157 (56.05%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNI-VLIHDDIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVNPSKNVFL 533
GM+IF K ++GK + FE E + D+K K+ EK G P +Q+LV+ G +L D ++D GI E+S +N+ + + + + +V +E+ K +++ D+K KI E + +EQ L+ ILE++RT+ Y + + +IN+V P +
Sbjct: 834 GGMQIFAKTMDGKTVAFEVRKAEKIRDVKLKIQEKIGLAPGEQRLVYQGGILEDERTVEDYGIAEKSVINVPARLRGGMQIFAKTMDGKTVAIEVQKTEKIRDIKLKIQEKIGLAPDEQILVYQGGILEDERTVEEYQIAEKSVINVVARPRSQLIW 990 HSP 2 Score: 98.2117 bits (243), Expect = 2.535e-23 Identity = 56/174 (32.18%), Postives = 95/174 (54.60%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHDDIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVIL-ENKRTLNSYNVKDNDIINLVVNPSKNVFLYIFGAIFAVILALEVAK 584
GM+IF K ++GK + FE E + D+K K+ EK G P +Q+LV+ G +L D ++D GI E+S + + + I + G+ V+ E+ K D + D+K KI E + +EQ L+ IL E++RT+ Y + + +IN+V P + ++ + I+ +EV K
Sbjct: 463 GGMQIFAKTMDGKTVAFEVRKAEKIRDVKLKIQEKIGLAPGEQRLVYQGGILEDERTVEDYGIAEKSVITVAIGGMVILAKTMDGKTVA--FEVQKTDTIRDVKLKIQEKVGVAPDEQILVYQGGILYEDERTVEEYQIAEKSVINVVARPRGGMQIFAI-TVDCKIVPIEVQK 633 HSP 3 Score: 85.5001 bits (210), Expect = 6.440e-19 Identity = 46/148 (31.08%), Postives = 81/148 (54.73%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHDDIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVV 509
GM+IF ++ K++ E +T+ D+K K+ EK G P +Q LV+ G +L D +D GI ++S +++V I D+ + G+ V +E+ K D + D+K +I + +E L+ ILE++RT Y + D +I++V
Sbjct: 238 GGMQIFAITVDCKIVPIEVQKTDTIRDVKLKIQEKVGVAPDEQILVYQGGILEDERTAEDYGIADESMIDVVAIR-DMQIFPKTGDGKIVPIEVQKTDTIRDVKLRIQNQIGLAPDEPKLVYQGGILEDERTAEDYGIADESMIDVVA 384 HSP 4 Score: 84.3445 bits (207), Expect = 1.515e-18 Identity = 50/164 (30.49%), Postives = 87/164 (53.05%), Query Frame = 3
Query: 45 TVFILTDSGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLH-DHDVLKDVGIVEQSTMNIVL-IHDDIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVNPSKNVF 530
+V + GM I K ++GK + FE +T+ D+K K+ EK G P +Q LV+ G +L+ D +++ I E+S +N+V + + + + V +E+ K D + D+K KI E + +EQ L+ ILE++RT Y + D +I++V +F
Sbjct: 154 SVITVAIGGMVILAKTMDGKTVAFEVQKTDTIRDVKLKIQEKVGVAPDEQILVYQGGILYEDERTVEEYQIAEKSVINVVARPRGGMQIFAITVDCKIVPIEVQKTDTIRDVKLKIQEKVGVAPDEQILVYQGGILEDERTAEDYGIADESMIDVVAIRDMQIF 317 HSP 5 Score: 83.1889 bits (204), Expect = 3.496e-18 Identity = 46/156 (29.49%), Postives = 86/156 (55.13%), Query Frame = 3
Query: 72 MKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHDDIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVIL-ENKRTLNSYNVKDNDIINLVVNPSKNVFLY 536
M+IF K +GK++ E +T+ D+K ++ + G P + KLV+ G +L D +D GI ++S +++V I + + + + +V E+ K D + D+K KI E + +EQ L+ IL E++RT+ Y + + +IN+V P + ++
Sbjct: 314 MQIFPKTGDGKIVPIEVQKTDTIRDVKLRIQNQIGLAPDEPKLVYQGGILEDERTAEDYGIADESMIDVVAIGGMVILAKTM-DGKTVAFEVQKTDTIRDVKLKIQEKVGVAPDEQILVYQGGILYEDERTVEEYQIAEKSVINVVARPRGGMQIF 468 HSP 6 Score: 81.6481 bits (200), Expect = 1.271e-17 Identity = 48/154 (31.17%), Postives = 83/154 (53.90%), Query Frame = 3
Query: 45 TVFILTDSGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLH-DHDVLKDVGIVEQSTMNIVL-IHDDIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIIN 500
+V + GM I K ++GK + FE +T+ D+K K+ EK G P +Q LV+ G +L+ D +++ I E+S +N+V + + + + V +E+ K D + D+K KI E + +EQ L+ ILE++RT Y + D +I+
Sbjct: 529 SVITVAIGGMVILAKTMDGKTVAFEVQKTDTIRDVKLKIQEKVGVAPDEQILVYQGGILYEDERTVEEYQIAEKSVINVVARPRGGMQIFAITVDCKIVPIEVQKTDTIRDVKLKIQEKVGVAPDEQILVYQGGILEDERTAEDYGIADESMID 682 HSP 7 Score: 80.8777 bits (198), Expect = 2.768e-17 Identity = 46/148 (31.08%), Postives = 81/148 (54.73%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLH-DHDVLKDVGIVEQSTMNIVL-IHDDIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINL 503
GM I K ++GK + FE +T+ D+K K+ EK G P +Q LV+ G +L+ D +++ I E+S +N+V + + + +V E+ K +++ D+K KI E + EQ L+ ILE++RT+ Y + + +IN+
Sbjct: 757 GGMVILAKTMDGKTVAFEVQKTDTIRDVKLKIQEKVGVAPDEQILVYQGGILYEDERTVEEYQIAEKSVINVVARPRGGMQIFAKTMDGKTVAFEVRKAEKIRDVKLKIQEKIGLAPGEQRLVYQGGILEDERTVEDYGIAEKSVINV 904 HSP 8 Score: 80.1073 bits (196), Expect = 5.127e-17 Identity = 45/151 (29.80%), Postives = 81/151 (53.64%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLH-DHDVLKDVGIVEQSTMNIVL-IHDDIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVN 512
GM I K ++GK + FE +T+ D+K K+ EK G P +Q LV+ G +L+ D +++ I E+S +N+V + + + +V E+ K +++ D+K KI E + EQ L+ ILE++RT+ Y + + +I + +
Sbjct: 386 GGMVILAKTMDGKTVAFEVQKTDTIRDVKLKIQEKVGVAPDEQILVYQGGILYEDERTVEEYQIAEKSVINVVARPRGGMQIFAKTMDGKTVAFEVRKAEKIRDVKLKIQEKIGLAPGEQRLVYQGGILEDERTVEDYGIAEKSVITVAIG 536 HSP 9 Score: 78.5666 bits (192), Expect = 1.569e-16 Identity = 45/161 (27.95%), Postives = 86/161 (53.42%), Query Frame = 3
Query: 57 LTDSGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHDDIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVIL-ENKRTLNSYNVKDNDIINLVVNPSKNVFLY 536
+ D G +N +GK++ E +T+ D+K ++ + G P + KLV+ G +L D +D GI ++S +++V I + + + + +V E+ K D + D+K KI E + +EQ L+ IL E++RT+ Y + + +IN+V P + ++
Sbjct: 680 MIDKGHANISQNGDGKIVPIEVQKTDTIRDVKLRIQNQIGLAPDEPKLVYQGGILEDERTAEDYGIADESMIDVVAIGGMVILAKTM-DGKTVAFEVQKTDTIRDVKLKIQEKVGVAPDEQILVYQGGILYEDERTVEEYQIAEKSVINVVARPRGGMQIF 839 HSP 10 Score: 77.0258 bits (188), Expect = 4.404e-16 Identity = 47/173 (27.17%), Postives = 90/173 (52.02%), Query Frame = 3
Query: 69 GMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHDDIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVIL-ENKRTLNSYNVKDNDIINLVVNPSKNVFLYIFGAIFAVILALEVAK 584
GM+++++ + G + + D+K ++ + G P +QKLV+ G +L D ++D GI E+S + + + I + G+ V+ E+ K D + D+K KI E + +EQ L+ IL E++RT+ Y + + +IN+V P + ++ + I+ +EV K
Sbjct: 89 GMQMYLRRVGGTTELVTVGKADKIRDVKLRIQNQIGLAPDEQKLVYQGGILEDERTVEDYGIAEKSVITVAIGGMVILAKTMDGKTVA--FEVQKTDTIRDVKLKIQEKVGVAPDEQILVYQGGILYEDERTVEEYQIAEKSVINVVARPRGGMQIFAI-TVDCKIVPIEVQK 258 HSP 11 Score: 76.2554 bits (186), Expect = 1.003e-15 Identity = 45/148 (30.41%), Postives = 80/148 (54.05%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHDDIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVV 509
GM+IF ++ K++ E +T+ D+K K+ EK G P +Q LV+ G +L D +D GI ++S ++ H +I+ + G+ V +E+ K D + D+K +I + +E L+ ILE++RT Y + D +I++V
Sbjct: 613 GGMQIFAITVDCKIVPIEVQKTDTIRDVKLKIQEKVGVAPDEQILVYQGGILEDERTAEDYGIADESMID--KGHANISQN---GDGKIVPIEVQKTDTIRDVKLRIQNQIGLAPDEPKLVYQGGILEDERTAEDYGIADESMIDVVA 755 HSP 12 Score: 56.9954 bits (136), Expect = 2.685e-9 Identity = 38/149 (25.50%), Postives = 75/149 (50.34%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHDDIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVN 512
GM+IFVK + +F + ++ + +K K+ + G + Q L +L D L+ GIV++S +NI L + +++ + V + K D++ D+K +I + +EQ L+ ILE++RT+ Y + + +I + +
Sbjct: 16 GGMRIFVKAWNENIFSFPAESDDKIYHLKSKIEKLLGIKMGNQILSNENEILEDEKTLEQYGIVDKSLINIEL---GMQMYLRRVGGTTELVTVGKADKIRDVKLRIQNQIGLAPDEQKLVYQGGILEDERTVEDYGIAEKSVITVAIG 161
BLAST of Polyubiquitin vs. Planmine SMEST
Match: SMESG000063621.1 (SMESG000063621.1) HSP 1 Score: 97.0561 bits (240), Expect = 7.778e-23 Identity = 49/158 (31.01%), Postives = 91/158 (57.59%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVL-IHDDIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVNPSKNVFLY 536
M+IF K ++G + FE ++T+ D+K+K+ EK G P +QKLV+ G +L D +++ I E+S +NIV + + + + +V E+ K +++ D+K KI E + +EQ L+ ILE++RT++ Y + + +IN+V P + ++
Sbjct: 1069 GSMQIFAKTMDGITVAFEVQKSDTIRDVKKKIQEKIGLAPDEQKLVYQGEILEDERTVEEYQIAEKSVINIVARLRWGMQIFAKTMDGKTVAFEVQKTEKISDIKKKIQEKIGLAPDEQKLVYQGGILEDERTVDEYQIAEKSVINIVARPRTAMQMF 1226 HSP 2 Score: 97.0561 bits (240), Expect = 7.778e-23 Identity = 49/158 (31.01%), Postives = 91/158 (57.59%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVL-IHDDIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVNPSKNVFLY 536
M+IF K ++G + FE ++T+ D+K+K+ EK G P +QKLV+ G +L D +++ I E+S +NIV + + + + +V E+ K +++ D+K KI E + +EQ L+ ILE++RT++ Y + + +IN+V P + ++
Sbjct: 1490 GSMQIFAKTMDGITVAFEVQKSDTIRDVKKKIQEKIGLAPDEQKLVYQGEILEDERTVEEYQIAEKSVINIVARLRWGMQIFAKTMDGKTVAFEVQKTEKISDIKKKIQEKIGLAPDEQKLVYQGGILEDERTVDEYQIAEKSVINIVARPRTAMQMF 1647 HSP 3 Score: 96.6709 bits (239), Expect = 9.875e-23 Identity = 49/157 (31.21%), Postives = 91/157 (57.96%), Query Frame = 3
Query: 69 GMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVL-IHDDIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVNPSKNVFLY 536
GM++F K + K + FE ++T+ D+K+K+ EK G P +QKLV+ G +L D +++ I E+S +NIV + + + + +V E+ K +++ D+K KI E + +EQ L+ ILE++RT++ Y + + +IN+V P + ++
Sbjct: 649 GMQMFAKTVNDKTVAFEVQKSDTIRDVKKKIQEKIGLAPDEQKLVYQGEILEDERTVEEYQIAEKSVINIVARLRWGMQIFAKTMDGKTVAFEVQKTEKISDIKKKIQEKIGLAPDEQKLVYQGGILEDERTVDEYQIAEKSVINIVARPRTAMQMF 805 HSP 4 Score: 96.2857 bits (238), Expect = 1.366e-22 Identity = 49/158 (31.01%), Postives = 89/158 (56.33%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVL-IHDDIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVNPSKNVFLY 536
G+KIF K ++GK + F+ + +T+ D+K+K+H K G P +Q LV+ G +L D + + I E+S +NI+ + + + ++V E+ K D + D+K KI E + +EQ L+ ILE++RT+ Y + + +IN+V P + ++
Sbjct: 80 GGVKIFGKTMDGKTIGFKVQMADTIRDVKKKIHGKIGLAPDEQILVYQGGILQDERTVDEYRIAEKSVINILARRRGSMQIFAKTMDGITVAFEVQKSDTIRDVKKKIQEKIGLAPDEQKLVYQGGILEDERTVEEYQIAEKSVINIVARPRTAMQMF 237 HSP 5 Score: 92.0485 bits (227), Expect = 4.001e-21 Identity = 49/150 (32.67%), Postives = 85/150 (56.67%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVL-IHDDIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVN 512
M+IF K ++G + FE ++T+ D+K+K+ EK G P +QKLV+ G +L D +++ I E+S +NIV + + + + +V E+ K D + D+K KI E + +EQ L+ ILE++RT+ Y + + +IN+V
Sbjct: 496 GSMQIFAKTMDGITVAFEVQKSDTIRDVKKKIQEKIGLAPDEQKLVYQGEILEDERTVEEYQIAEKSVINIVARLRTAMQMFAKTVNDKTVAFEVQKSDTIRDVKKKIQEKIGLAPDEQKLVYQGEILEDERTVEEYQIAEKSVINIVAR 645 HSP 6 Score: 86.6557 bits (213), Expect = 2.542e-19 Identity = 56/174 (32.18%), Postives = 90/174 (51.72%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHDDIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVNPSKNVFLYIFGAIF-AVILALEVAK 584
M IF K ++GK + FE +T+ D+K K+ E+ G +QKLV+ G L D ++D GI + S +N+V I + + + ++V E+ K D + D+K KI E + +EQ L+ ILE++RT+ Y + + II +V + IF +ALEV K
Sbjct: 1741 GSMLIFAKTMDGKTVAFEVQKTDTIRDVKEKIQEEIGLASDEQKLVYQGQDLEDEISVEDYGIADGSVINVVDIG-SMQIFAKTMDGITVAFEVQKSDTIRDVKKKIQEKIGLAPDEQKLVYQGEILEDERTVEEYQIAEKSIIYIVARLRWG--MQIFAKTMDGKTVALEVQK 1911 HSP 7 Score: 86.2705 bits (212), Expect = 3.580e-19 Identity = 46/137 (33.58%), Postives = 79/137 (57.66%), Query Frame = 3
Query: 69 GMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHDDIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNV 479
GM+IF K ++GK + E +E + DIK+K+ EK G P +QKLV+ G L D ++D GI ++S +N+ ++ + ++V + SV + + DQ+ +LK KI E + E QTL+ +L++ +T Y +
Sbjct: 1892 GMQIFAKTMDGKTVALEVQKSEKISDIKKKIQEKIGLAPDEQKLVYQGQDLEDEISVEDYGIADKSVINVDILRKLMEIYVKKCDGKSVTIPVHNTDQIGNLKVKIEEKFGLAPESQTLVYQGDVLKDDKTYQEYQI 2028 HSP 8 Score: 80.8777 bits (198), Expect = 2.316e-17 Identity = 44/147 (29.93%), Postives = 83/147 (56.46%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVL-IHDDIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINL 503
M+IF K ++G + FE ++T+ D+K+K+ EK G P +QKLV+ G +L D +++ I E+S + IV + + + + +V +E+ K +++ D+K KI E + +EQ L+ LE++ ++ Y + D +IN+
Sbjct: 1815 GSMQIFAKTMDGITVAFEVQKSDTIRDVKKKIQEKIGLAPDEQKLVYQGEILEDERTVEEYQIAEKSIIYIVARLRWGMQIFAKTMDGKTVALEVQKSEKISDIKKKIQEKIGLAPDEQKLVYQGQDLEDEISVEDYGIADKSVINV 1961 HSP 9 Score: 75.8702 bits (185), Expect = 1.334e-15 Identity = 46/139 (33.09%), Postives = 74/139 (53.24%), Query Frame = 3
Query: 69 GMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHDDIAVHVLIGE--NVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNV 479
GM+IF K ++GK + FE E + DIK+K+ EK G P +QKLV+ G +L D + + I E+S +NIV A+ + + +V E+ K D + D+K KI + +EQ L LE++ ++ +V
Sbjct: 725 GMQIFAKTMDGKTVAFEVQKTEKISDIKKKIQEKIGLAPDEQKLVYQGGILEDERTVDEYQIAEKSVINIVA-RPRTAMQMFAKTVNDKTVAFEVQKADTIRDVKKKIQRKFWLPPDEQKLDYEGQDLEDELSIAERSV 862 HSP 10 Score: 75.8702 bits (185), Expect = 1.334e-15 Identity = 46/139 (33.09%), Postives = 74/139 (53.24%), Query Frame = 3
Query: 69 GMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHDDIAVHVLIGE--NVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNV 479
GM+IF K ++GK + FE E + DIK+K+ EK G P +QKLV+ G +L D + + I E+S +NIV A+ + + +V E+ K D + D+K KI + +EQ L LE++ ++ +V
Sbjct: 1146 GMQIFAKTMDGKTVAFEVQKTEKISDIKKKIQEKIGLAPDEQKLVYQGGILEDERTVDEYQIAEKSVINIVA-RPRTAMQMFAKTVNDKTVAFEVQKADTIRDVKKKIQRKFWLPPDEQKLDYEGQDLEDELSIAERSV 1283 HSP 11 Score: 75.8702 bits (185), Expect = 1.334e-15 Identity = 46/139 (33.09%), Postives = 74/139 (53.24%), Query Frame = 3
Query: 69 GMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHDDIAVHVLIGE--NVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNV 479
GM+IF K ++GK + FE E + DIK+K+ EK G P +QKLV+ G +L D + + I E+S +NIV A+ + + +V E+ K D + D+K KI + +EQ L LE++ ++ +V
Sbjct: 1567 GMQIFAKTMDGKTVAFEVQKTEKISDIKKKIQEKIGLAPDEQKLVYQGGILEDERTVDEYQIAEKSVINIVA-RPRTAMQMFAKTVNDKTVAFEVQKADTIRDVKKKIQRKFWLPPDEQKLDYEGQDLEDELSIAERSV 1704 HSP 12 Score: 75.8702 bits (185), Expect = 1.411e-15 Identity = 47/177 (26.55%), Postives = 86/177 (48.59%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVL--------------------KDVGIVEQSTMNIVLIHDDIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVNPSKNVFLY 536
+ M++F K + K + FE +T+ D+K+K+ K P +QKL + G L D + +D GI + S +N+V I + + + ++V E+ K D + D+K KI E + +EQ L+ ILE++RT++ Y + + +IN+V P + ++
Sbjct: 232 TAMQMFAKTVNDKTVAFEVQKADTIRDVKKKIQRKFWLPPDEQKLDYEGQDLEDELSIAERSVDEISVDEISVDEISVEDYGIADGSVINVVDIG-SMQIFAKTMDGITVAFEVQKSDTIRDVKKKIQEKIGLAPDEQKLVYQGGILEDERTVDEYQIAEKSVINIVARPRTAMQMF 407 HSP 13 Score: 73.559 bits (179), Expect = 8.416e-15 Identity = 54/194 (27.84%), Postives = 92/194 (47.42%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVL-IHDDIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILEN-----KRTLNS---------------YNVKDNDIINLVVNPSKNVFLYIFGAIFAVILALEVAK 584
M+IF K ++G + FE ++T+ D+K+K+ EK G P +QKLV+ G +L D +++ I E+S +NIV + + + + +V E+ K D + D+K KI + +EQ L LE+ +R+++ Y + D +IN+V S +F I +A EV K
Sbjct: 899 GSMQIFAKTMDGITVAFEVQKSDTIRDVKKKIQEKIGLAPDEQKLVYQGEILEDERTVEEYQIAEKSVINIVARLRTAMQMFAKTVNDKTVAFEVQKADTIRDVKKKIQRKFWLPPDEQKLDYEGQDLEDELSIAERSVDEISVDEISVDEISVEDYGIADGSVINVVDIGSMQIFAKTMDGI---TVAFEVQK 1089 HSP 14 Score: 73.559 bits (179), Expect = 8.416e-15 Identity = 54/194 (27.84%), Postives = 92/194 (47.42%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVL-IHDDIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILEN-----KRTLNS---------------YNVKDNDIINLVVNPSKNVFLYIFGAIFAVILALEVAK 584
M+IF K ++G + FE ++T+ D+K+K+ EK G P +QKLV+ G +L D +++ I E+S +NIV + + + + +V E+ K D + D+K KI + +EQ L LE+ +R+++ Y + D +IN+V S +F I +A EV K
Sbjct: 1320 GSMQIFAKTMDGITVAFEVQKSDTIRDVKKKIQEKIGLAPDEQKLVYQGEILEDERTVEEYQIAEKSVINIVARLRTAMQMFAKTVNDKTVAFEVQKADTIRDVKKKIQRKFWLPPDEQKLDYEGQDLEDELSIAERSVDEISVDEISVDEISVEDYGIADGSVINVVDIGSMQIFAKTMDGI---TVAFEVQK 1510 HSP 15 Score: 73.1738 bits (178), Expect = 1.046e-14 Identity = 55/195 (28.21%), Postives = 92/195 (47.18%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHDDIAVHVLIGE--NVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILEN-----KRTLNS---------------YNVKDNDIINLVVNPSKNVFLYIFGAIFAVILALEVAK 584
M+IF K ++G + FE ++T+ D+K+K+ EK G P +QKLV+ G +L D +++ I E+S +NIV A+ + + +V E+ K D + D+K KI + +EQ L LE+ +R+++ Y + D +IN+V S +F I +A EV K
Sbjct: 156 GSMQIFAKTMDGITVAFEVQKSDTIRDVKKKIQEKIGLAPDEQKLVYQGGILEDERTVEEYQIAEKSVINIVA-RPRTAMQMFAKTVNDKTVAFEVQKADTIRDVKKKIQRKFWLPPDEQKLDYEGQDLEDELSIAERSVDEISVDEISVDEISVEDYGIADGSVINVVDIGSMQIFAKTMDGI---TVAFEVQK 346 HSP 16 Score: 72.4034 bits (176), Expect = 1.824e-14 Identity = 55/195 (28.21%), Postives = 91/195 (46.67%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVLKDVGIVEQSTMNIVLIHDDIAVHVLIGE--NVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILEN-----KRTLNS---------------YNVKDNDIINLVVNPSKNVFLYIFGAIFAVILALEVAK 584
M+IF K ++G + FE ++T+ D+K+K+ EK G P +QKLV+ G +L D + + I E+S +NIV A+ + + +V E+ K D + D+K KI + +EQ L LE+ +R+++ Y + D +IN+V S +F I +A EV K
Sbjct: 326 GSMQIFAKTMDGITVAFEVQKSDTIRDVKKKIQEKIGLAPDEQKLVYQGGILEDERTVDEYQIAEKSVINIVA-RPRTAMQMFAKTVNDKTVAFEVQKADTIRDVKKKIQRKFWLPPDEQKLDYEGQDLEDELSIAERSVDEISVDEISVDEISVEDYGIADGSVINVVDIGSMQIFAKTMDGI---TVAFEVQK 516 HSP 17 Score: 68.9366 bits (167), Expect = 3.257e-13 Identity = 46/174 (26.44%), Postives = 81/174 (46.55%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVL-------------------------KDVGIVEQSTMNIVLIHDDIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVN 512
+ M++F K + K + FE +T+ D+K+K+ K P +QKL + G L D + +D GI + S +N+V I + + + ++V E+ K D + D+K KI E + +EQ L+ ILE++RT+ Y + + +IN+V
Sbjct: 800 TAMQMFAKTVNDKTVAFEVQKADTIRDVKKKIQRKFWLPPDEQKLDYEGQDLEDELSIAERSVDEISVDEISVDEISVDEISVEDYGIADGSVINVVDIG-SMQIFAKTMDGITVAFEVQKSDTIRDVKKKIQEKIGLAPDEQKLVYQGEILEDERTVEEYQIAEKSVINIVAR 972 HSP 18 Score: 68.9366 bits (167), Expect = 3.257e-13 Identity = 46/174 (26.44%), Postives = 81/174 (46.55%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVL-------------------------KDVGIVEQSTMNIVLIHDDIAVHVLIGENVSVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVN 512
+ M++F K + K + FE +T+ D+K+K+ K P +QKL + G L D + +D GI + S +N+V I + + + ++V E+ K D + D+K KI E + +EQ L+ ILE++RT+ Y + + +IN+V
Sbjct: 1221 TAMQMFAKTVNDKTVAFEVQKADTIRDVKKKIQRKFWLPPDEQKLDYEGQDLEDELSIAERSVDEISVDEISVDEISVDEISVEDYGIADGSVINVVDIG-SMQIFAKTMDGITVAFEVQKSDTIRDVKKKIQEKIGLAPDEQKLVYQGEILEDERTVEEYQIAEKSVINIVAR 1393 HSP 19 Score: 62.3882 bits (150), Expect = 4.913e-11 Identity = 52/201 (25.87%), Postives = 89/201 (44.28%), Query Frame = 3
Query: 66 SGMKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLHDHDVL-------------------------KDVGIVEQSTMNIVLIHDDIAVHVLIGENV---SVNVEIAKGDQVIDLKTKIYELKHIKVEEQTLLLNAVILENKRTLNSYNVKDNDIINLVVNPSKNVFLYIFGAIFAVILALEVAK 584
+ M++F K + K + FE +T+ D+K+K+ K P +QKL + G L D + +D GI + S +N+V DI ++ + + +V E+ K D + D+K KI E + +EQ L+ LE++ ++ Y + D +IN+V S +F I +A EV K
Sbjct: 1642 TAMQMFAKTVNDKTVAFEVQKADTIRDVKKKIQRKFWLPPDEQKLDYEGQDLEDELSIAERSVDEISVDEISVDEISVDEISVEDYGIADGSVINVV----DIGSMLIFAKTMDGKTVAFEVQKTDTIRDVKEKIQEEIGLASDEQKLVYQGQDLEDEISVEDYGIADGSVINVVDIGSMQIFAKTMDGI---TVAFEVQK 1835 The following BLAST results are available for this feature:
BLAST of Polyubiquitin vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Polyubiquitin vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 4
BLAST of Polyubiquitin vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of Polyubiquitin vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of Polyubiquitin vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 3
BLAST of Polyubiquitin vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of Polyubiquitin vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Polyubiquitin vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Polyubiquitin vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 1
BLAST of Polyubiquitin vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 3
BLAST of Polyubiquitin vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 1
BLAST of Polyubiquitin vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 2
BLAST of Polyubiquitin vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 1
BLAST of Polyubiquitin vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30029792 ID=SMED30029792|Name=Polyubiquitin|organism=Schmidtea mediterranea sexual|type=transcript|length=697bpback to top protein sequence of SMED30029792-orf-1 >SMED30029792-orf-1 ID=SMED30029792-orf-1|Name=SMED30029792-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=176bp MKIFVKNLEGKVLTFESNLNETVLDIKRKVHEKTGDEPKKQKLVFHGSVLback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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