transmembrane emp24 domain-containing protein 2-like

Overview
Nametransmembrane emp24 domain-containing protein 2-like
Smed IDSMED30029791
Length (bp)653
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of transmembrane emp24 domain-containing protein 2-like (SMED30029791) t-SNE clustered cells

Violin plots show distribution of expression levels for transmembrane emp24 domain-containing protein 2-like (SMED30029791) in cells (dots) of each of the 12 neoblast clusters.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Homology
BLAST of transmembrane emp24 domain-containing protein 2-like vs. Ensembl Human
Match: TMED2 (transmembrane p24 trafficking protein 2 [Source:HGNC Symbol;Acc:HGNC:16996])

HSP 1 Score: 121.324 bits (303), Expect = 7.613e-34
Identity = 65/185 (35.14%), Postives = 100/185 (54.05%), Query Frame = 1
Query:   70 SSFEINLNPNSEECLFDYFNKSDKVEFSYEVINGGFLDIESKLLSPMKVEFMRRDKGSYGVYDLVIEQSGLYYFCLINKEGSQVEKTVRFNLFSEPHYRWERNITSRESKNSDLEEIVHDLSFLMHSVKAESEYSLTREQIHDQANRSANRHFLLWGLFEMVLMVAMSLGQIAYLKHLFEVRRFV 624
            S + ++++ ++EEC F+      K+   +EV  GGFLDI+ ++  P      + D+ S G Y       G Y FC  N+  +   K V F +      + +   T  E+  + LEE++++L+  M +VK E EY   RE+IH   N + N   +LW  FE +++VAM+LGQI YLK  FEVRR V
Sbjct:   19 SGYFVSIDAHAEECFFERVTSGTKMGLIFEVAEGGFLDIDVEITGPDNKGIYKGDRESSGKYTFAAHMDGTYKFCFSNRMSTMTPKIVMFTIDIGEAPKGQDMET--EAHQNKLEEMINELAVAMTAVKHEQEYMEVRERIHRAINDNTNSRVVLWSFFEALVLVAMTLGQIYYLKRFFEVRRVV 201          
BLAST of transmembrane emp24 domain-containing protein 2-like vs. Ensembl Human
Match: TMED2 (transmembrane p24 trafficking protein 2 [Source:HGNC Symbol;Acc:HGNC:16996])

HSP 1 Score: 83.9593 bits (206), Expect = 2.578e-20
Identity = 46/116 (39.66%), Postives = 66/116 (56.90%), Query Frame = 1
Query:  277 GLYYFCLINKEGSQVEKTVRFNLFSEPHYRWERNITSRESKNSDLEEIVHDLSFLMHSVKAESEYSLTREQIHDQANRSANRHFLLWGLFEMVLMVAMSLGQIAYLKHLFEVRRFV 624
            G Y FC  N+  +   K V F +      + +   T  E+  + LEE++++L+  M +VK E EY   RE+IH   N + N   +LW  FE +++VAM+LGQI YLK  FEVRR V
Sbjct:    3 GTYKFCFSNRMSTMTPKIVMFTIDIGEAPKGQDMET--EAHQNKLEEMINELAVAMTAVKHEQEYMEVRERIHRAINDNTNSRVVLWSFFEALVLVAMTLGQIYYLKRFFEVRRVV 116          
BLAST of transmembrane emp24 domain-containing protein 2-like vs. Ensembl Human
Match: TMED2 (transmembrane p24 trafficking protein 2 [Source:HGNC Symbol;Acc:HGNC:16996])

HSP 1 Score: 75.8702 bits (185), Expect = 1.016e-16
Identity = 43/147 (29.25%), Postives = 74/147 (50.34%), Query Frame = 1
Query:   70 SSFEINLNPNSEECLFDYFNKSDKVEFSYEVINGGFLDIESKLLSPMKVEFMRRDKGSYGVYDLVIEQSGLYYFCLINKEGSQVEKTVRF--NLFSEPHYR---WERNITSRESKNSDLEEIVHDLSFLMHSVKAESEYSLTREQIH 495
            S + ++++ ++EEC F+      K+   +EV  GGFLDI+ ++  P      + D+ S G Y       G Y FC  N+  +   K V F  ++   P  +    E    + ++  + LEE++++L+  M +VK E EY   RE+IH
Sbjct:   19 SGYFVSIDAHAEECFFERVTSGTKMGLIFEVAEGGFLDIDVEITGPDNKGIYKGDRESSGKYTFAAHMDGTYKFCFSNRMSTMTPKIVMFTIDIGEAPKGQDMETEGGGDTWDAHQNKLEEMINELAVAMTAVKHEQEYMEVRERIH 165          
BLAST of transmembrane emp24 domain-containing protein 2-like vs. Ensembl Human
Match: TMED7 (transmembrane p24 trafficking protein 7 [Source:HGNC Symbol;Acc:HGNC:24253])

HSP 1 Score: 58.151 bits (139), Expect = 9.025e-10
Identity = 42/189 (22.22%), Postives = 79/189 (41.80%), Query Frame = 1
Query:   61 NNLSSFEINLNPNSEECLFDYFNKSDKVEFSYEVINGGFLDIESKLLSPMKVEFMRRDKGSYGVYDLVIEQSGLYYFCLINKEGSQVEKTVRFNLF---SEPHYRWERNITSRESKNSDLEEIVHDLSFLMHSVKAESEYSLTREQIHDQANRSANRHFLLWGLFEMVLMVAMSLGQIAYLKHLFEVRR 618
               S     L  N+++C ++   +  K    ++VI GG  D++ +L  P      +  K  Y  +     ++G Y FC  N+  +   KTV F+       P +  E  +++     S    I H+   L   +  ++ + L   Q   +A    N     W + E ++++ +S+GQ+  LK  F  +R
Sbjct:   32 GGASEITFELPDNAKQCFYEDIAQGTKCTLEFQVITGGHYDVDCRLEDPDGKVLYKEMKKQYDSFTFTASKNGTYKFCFSNEFSTFTHKTVYFDFQVGEDPPLFPSENRVSALTQMESACVSI-HEA--LKSVIDYQTHFRLREAQGRSRAE-DLNTRVAYWSVGEALILLVVSIGQVFLLKSFFSDKR 216          
BLAST of transmembrane emp24 domain-containing protein 2-like vs. Ensembl Human
Match: TMED3 (transmembrane p24 trafficking protein 3 [Source:HGNC Symbol;Acc:HGNC:28889])

HSP 1 Score: 52.373 bits (124), Expect = 7.813e-8
Identity = 41/180 (22.78%), Postives = 74/180 (41.11%), Query Frame = 1
Query:   88 LNPNSEECLFDYFNKSDKVEFSYEVINGGFLDIESKLLSPMKVEFMRRDKGSYGVYDLVIEQSGLYYFCLINKEGSQVEKTVRFNLFSEPHYRWERNITSRESKNSDLEE---IVHDLSFLMHSVKAESEYSLTREQIHDQANRSANRHFLLWGLFEMVLMVAMSLGQIAYLKHLFEVRR 618
            L  N+++C  +   +  K    Y+VI GG  D++  +  P      R  K  Y  +    E  G+Y FC  N+  +   KTV F+           ++ +R +  + +E     +H+   L   + +++ Y L   Q   +A    N     W + E + +  +S  Q+  LK  F  +R
Sbjct:   33 LPDNAKQCFHEEVEQGVKFSLDYQVITGGHYDVDCYVEDPQGNTIYRETKKQYDSFTYRAEVKGVYQFCFSNEFSTFSHKTVYFDFQVGDEPPILPDMGNRVTALTQMESACVTIHEA--LKTVIDSQTHYRLREAQDRARAE-DLNSRVSYWSVGETIALFVVSFSQVLLLKSFFTEKR 209          
BLAST of transmembrane emp24 domain-containing protein 2-like vs. Ensembl Celegans
Match: sel-9 (Suppressor/enhancer of lin-12 protein 9 [Source:UniProtKB/Swiss-Prot;Acc:O17528])

HSP 1 Score: 125.561 bits (314), Expect = 8.542e-36
Identity = 71/205 (34.63%), Postives = 108/205 (52.68%), Query Frame = 1
Query:   16 MNKLHFALFVHIIIINNLSSFEINLNPNSEECLFDYFNKSDKVEFSYEVINGGFLDIESKLLSPMKVEFMRRDKGSYGVYDLVIEQSGLYYFCLINKEGSQVEKTVRFNL-FSEPHYRWE-RNITSRESKNSDLEEIVHDLSFLMHSVKAESEYSLTREQIHDQANRSANRHFLLWGLFEMVLMVAMSLGQIAYLKHLFEVRRFV 624
            MN L + L V  + +   +S+ I+++ N E+C FD      K+   +EV  GGFLDI+ K+  P   E  + ++ S G +       G+Y +C  NK  +   K V F +  +EPH +          + N+ LEE+V +LS  + SVK E EY   RE++H   N + N   ++W  FE  ++V M++GQI YLK  FEVR  V
Sbjct:    1 MNSLTWILAV--LFVTPAASYFIHVDANEEQCFFDRLTSGTKMGLMFEVAEGGFLDIDVKITGPDNKEIYKGERESSGKFTFAAHMDGVYTYCFGNKMSTMTPKAVMFTVEITEPHQQAPGAAANQDAADNAKLEEMVRELSSALMSVKHEQEYMEVRERVHRNINENTNSRVVMWAAFEAFVLVGMTVGQIFYLKRFFEVRTMV 203          
BLAST of transmembrane emp24 domain-containing protein 2-like vs. Ensembl Celegans
Match: F57B10.5 (pep chromosome:WBcel235:I:6562699:6563660:1 gene:WBGene00019003.1 transcript:F57B10.5.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:F57B10.5)

HSP 1 Score: 47.7506 bits (112), Expect = 1.151e-6
Identity = 41/201 (20.40%), Postives = 77/201 (38.31%), Query Frame = 1
Query:   25 LHFALFVHIIIINNLS---SFEINLNPNSEECLFDYFNKSDKVEFSYEVINGGFLDIESKLLSPMKVEFMRRDKGSYGVYDLVIEQSGLYYFCLINKEGSQVEKTVRFNLFSEPHYRWERNITSRESKNSDLEEIVHDLSFLMHSVKAESEYSLTREQIHDQANRSANRHFLLWGLFEMVLMVAMSLGQIAYLKHLFEVRR 618
            L F +   I++   LS   +FE  L  N+ +C ++   K     F ++V+ GG  D++  +  P      +  K  Y   +   E  G Y  C  N+  +   K V  +            +T      + LE     +   + ++     +   RE    +     N   ++W L +  ++V + +GQ+  LK  F  +R
Sbjct:    2 LKFVIVSSILVALGLSIELTFE--LPDNANQCFYEDLKKDVDTVFEFQVVTGGHYDVDLIIEDPNGKVLYKDTKKQYDSINFKAEVEGTYKACFSNEFSTFSHKIVYMDWQFGDQNALHAAVTQGAHAMTQLENYAVAIGDKLRTIDDYQTHHRLREATGRKRAEELNERVMIWSLGQSAVVVFIGIGQVFLLKSFFNDKR 200          
BLAST of transmembrane emp24 domain-containing protein 2-like vs. Ensembl Fly
Match: CHOp24 (gene:FBgn0029709 transcript:FBtr0343566)

HSP 1 Score: 115.931 bits (289), Expect = 6.706e-32
Identity = 62/185 (33.51%), Postives = 99/185 (53.51%), Query Frame = 1
Query:   73 SFEINLNPNSEECLFDYFNKSDKVEFSYEVINGGFLDIESKLLSPMKVEFMRRDKGSYGVYDLVIEQSGLYYFCLINKEGSQVEKTVRFNL-FSEPHYRWERNITSRESKNSDLEEIVHDLSFLMHSVKAESEYSLTREQIHDQANRSANRHFLLWGLFEMVLMVAMSLGQIAYLKHLFEVRRFV 624
            +F ++++ ++EEC F+      K   ++EVI+GGFLD++ K+  P        +K S G Y  V    G Y  C  N+  S   K V F++   +   R        E  ++ LE+++ +LS  + SVK E EY   R++IH   N + N   +LW  FE +++V M++GQ+ YLK  FEV+R V
Sbjct:   24 AFIVSVDAHNEECFFENVEGGTKFGVTFEVIDGGFLDVDIKISGPDNHVMHESEKESSGKYTFVAPAKGTYTVCFNNERSSMTPKLVMFSIDVGDAPQRAPGAPGEEEVGHTKLEDMIRELSGTLTSVKHEQEYMHVRDKIHRSVNENTNSRVVLWSTFEALVLVLMTVGQVYYLKRFFEVKRVV 208          
BLAST of transmembrane emp24 domain-containing protein 2-like vs. Ensembl Fly
Match: CHOp24 (gene:FBgn0029709 transcript:FBtr0070675)

HSP 1 Score: 115.931 bits (289), Expect = 6.706e-32
Identity = 62/185 (33.51%), Postives = 99/185 (53.51%), Query Frame = 1
Query:   73 SFEINLNPNSEECLFDYFNKSDKVEFSYEVINGGFLDIESKLLSPMKVEFMRRDKGSYGVYDLVIEQSGLYYFCLINKEGSQVEKTVRFNL-FSEPHYRWERNITSRESKNSDLEEIVHDLSFLMHSVKAESEYSLTREQIHDQANRSANRHFLLWGLFEMVLMVAMSLGQIAYLKHLFEVRRFV 624
            +F ++++ ++EEC F+      K   ++EVI+GGFLD++ K+  P        +K S G Y  V    G Y  C  N+  S   K V F++   +   R        E  ++ LE+++ +LS  + SVK E EY   R++IH   N + N   +LW  FE +++V M++GQ+ YLK  FEV+R V
Sbjct:   24 AFIVSVDAHNEECFFENVEGGTKFGVTFEVIDGGFLDVDIKISGPDNHVMHESEKESSGKYTFVAPAKGTYTVCFNNERSSMTPKLVMFSIDVGDAPQRAPGAPGEEEVGHTKLEDMIRELSGTLTSVKHEQEYMHVRDKIHRSVNENTNSRVVLWSTFEALVLVLMTVGQVYYLKRFFEVKRVV 208          
BLAST of transmembrane emp24 domain-containing protein 2-like vs. Ensembl Fly
Match: CG9308 (gene:FBgn0034681 transcript:FBtr0071749)

HSP 1 Score: 83.9593 bits (206), Expect = 8.516e-20
Identity = 48/200 (24.00%), Postives = 94/200 (47.00%), Query Frame = 1
Query:   34 ALFVHIIIINNLSSFEINLNPNSEECLFDYFNKSDKVEFSYEVINGGFLDIESKLLSPMKVEFMRRDKGSYGVYDLVIEQSGLYYFCLINKEGSQVEKTVRFNLFSEPHYRWERNITSRES---KNSDLEEIVHDLSFLMHSVKAESEYSLTREQIHDQANRSANRHFLLWGLFEMVLMVAMSLGQIAYLKHLFEVRRFV 624
            A+ + +++      F + L+ +   C +D+ N SDKV  S+EV+ GGF D+  ++  P         + + G +     + G Y  C  NK  +   K + F         +  + + R     + + ++ +++ LS  + +VK E EY   R + H + +       L W +F  ++++  ++ ++ YLKH FEV+R V
Sbjct:    4 AIVLVLVLFQAACGFIVTLDAHETMCFYDHANVSDKVTVSFEVMEGGFKDVGVEIAGPDDDRLHHSKQDTMGSFTFTAMKEGRYQLCFDNKMSTMTPKILMFQFHVARAIEFYMDSSKRVDDVIEQATVQSMINQLSAKLGAVKMEQEYMHFRYRGHLEVSDMVELRVLAWSIFGPMMLIITAVLEVYYLKHFFEVKRVV 203          
BLAST of transmembrane emp24 domain-containing protein 2-like vs. Ensembl Fly
Match: p24-1 (gene:FBgn0030341 transcript:FBtr0073604)

HSP 1 Score: 56.225 bits (134), Expect = 1.459e-9
Identity = 45/201 (22.39%), Postives = 88/201 (43.78%), Query Frame = 1
Query:   34 ALFVHIIIINNLSSFEINLNPNSEECLFDYFNKSDKVEFSYEVINGGFLDIESKLLSPMKVEFMRRDKGSYGVYDLVIEQSGLYYFCLINKEGSQVEKT--VRFNLFSEPHYRW----ERNITSRESKNSDLEEIVHDLSFLMHSVKAESEYSLTREQIHDQANRSANRHFLLWGLFEMVLMVAMSLGQIAYLKHLFEVRR 618
            AL +H I   +   F  +L  N+ +C ++   K+    F ++V  GG LD++  L  P        +K ++  +  V E +G+Y  C  N+  +   K   V F +  EP           +T  E+ +  + + ++D+      + A++ + L   Q   +A    N+  ++W   E   ++ + L QI  L++ F  R+
Sbjct:   11 ALMMHCI---SAVEFTFDLADNAVDCFYEEIKKNSSAYFEFQVSAGGQLDVDVTLKDPQGKVIYSLEKATFDSHQFVAETTGVYTACFGNQFSAFSHKIVYVDFQVGEEPALPGVDEHATVLTQMETSSQAIHKGLNDI------LDAQTHHRLREAQGRKRA-EDLNQRVMVWSSLETAAVIVIGLVQIMVLRNFFTDRK 201          
BLAST of transmembrane emp24 domain-containing protein 2-like vs. Ensembl Fly
Match: p24-1 (gene:FBgn0030341 transcript:FBtr0334455)

HSP 1 Score: 56.225 bits (134), Expect = 1.459e-9
Identity = 45/201 (22.39%), Postives = 88/201 (43.78%), Query Frame = 1
Query:   34 ALFVHIIIINNLSSFEINLNPNSEECLFDYFNKSDKVEFSYEVINGGFLDIESKLLSPMKVEFMRRDKGSYGVYDLVIEQSGLYYFCLINKEGSQVEKT--VRFNLFSEPHYRW----ERNITSRESKNSDLEEIVHDLSFLMHSVKAESEYSLTREQIHDQANRSANRHFLLWGLFEMVLMVAMSLGQIAYLKHLFEVRR 618
            AL +H I   +   F  +L  N+ +C ++   K+    F ++V  GG LD++  L  P        +K ++  +  V E +G+Y  C  N+  +   K   V F +  EP           +T  E+ +  + + ++D+      + A++ + L   Q   +A    N+  ++W   E   ++ + L QI  L++ F  R+
Sbjct:   11 ALMMHCI---SAVEFTFDLADNAVDCFYEEIKKNSSAYFEFQVSAGGQLDVDVTLKDPQGKVIYSLEKATFDSHQFVAETTGVYTACFGNQFSAFSHKIVYVDFQVGEEPALPGVDEHATVLTQMETSSQAIHKGLNDI------LDAQTHHRLREAQGRKRA-EDLNQRVMVWSSLETAAVIVIGLVQIMVLRNFFTDRK 201          
BLAST of transmembrane emp24 domain-containing protein 2-like vs. Ensembl Zebrafish
Match: tmed2 (transmembrane p24 trafficking protein 2 [Source:ZFIN;Acc:ZDB-GENE-030131-269])

HSP 1 Score: 125.561 bits (314), Expect = 1.076e-35
Identity = 66/185 (35.68%), Postives = 103/185 (55.68%), Query Frame = 1
Query:   70 SSFEINLNPNSEECLFDYFNKSDKVEFSYEVINGGFLDIESKLLSPMKVEFMRRDKGSYGVYDLVIEQSGLYYFCLINKEGSQVEKTVRFNLFSEPHYRWERNITSRESKNSDLEEIVHDLSFLMHSVKAESEYSLTREQIHDQANRSANRHFLLWGLFEMVLMVAMSLGQIAYLKHLFEVRRFV 624
            S + ++++ ++EEC ++  N   K+   +EV  GGFLDI+ K+  P   +  + D+ S G Y +     G Y FC  N+  +   K V F +      + +   T  E+  + LEE++++L+  M +VK E EY   RE+IH   N + N   +LW  FE +++VAM+LGQI YLK  FEVRR V
Sbjct:   19 SGYFVSIDAHAEECFYERVNSGTKMSLMFEVAEGGFLDIDVKITGPDGKQIYKGDRESSGKYSVAAHMDGTYKFCFSNEMSTMTPKIVMFTIDIGEAPKGQDMET--EAHQNKLEEMINELAVAMTAVKHEQEYMEVRERIHRAINDNTNSRVVLWSFFEALVLVAMTLGQIYYLKRFFEVRRVV 201          
BLAST of transmembrane emp24 domain-containing protein 2-like vs. Ensembl Zebrafish
Match: tmed2 (transmembrane p24 trafficking protein 2 [Source:ZFIN;Acc:ZDB-GENE-030131-269])

HSP 1 Score: 124.79 bits (312), Expect = 2.586e-35
Identity = 67/190 (35.26%), Postives = 105/190 (55.26%), Query Frame = 1
Query:   70 SSFEINLNPNSEECLFDYFNKSDKVEFSYEVINGGFLDIESKLLSPMKVEFMRRDKGSYGVYDLVIEQSGLYYFCLINKEGSQVEKTVRF--NLFSEPHYR---WERNITSRESKNSDLEEIVHDLSFLMHSVKAESEYSLTREQIHDQANRSANRHFLLWGLFEMVLMVAMSLGQIAYLKHLFEVRRFV 624
            S + ++++ ++EEC ++  N   K+   +EV  GGFLDI+ K+  P   +  + D+ S G Y +     G Y FC  N+  +   K V F  ++   P  +    E    S ++  + LEE++++L+  M +VK E EY   RE+IH   N + N   +LW  FE +++VAM+LGQI YLK  FEVRR V
Sbjct:   19 SGYFVSIDAHAEECFYERVNSGTKMSLMFEVAEGGFLDIDVKITGPDGKQIYKGDRESSGKYSVAAHMDGTYKFCFSNEMSTMTPKIVMFTIDIGEAPKGQDMETEGGGDSWDAHQNKLEEMINELAVAMTAVKHEQEYMEVRERIHRAINDNTNSRVVLWSFFEALVLVAMTLGQIYYLKRFFEVRRVV 208          
BLAST of transmembrane emp24 domain-containing protein 2-like vs. Ensembl Zebrafish
Match: tmed7 (transmembrane p24 trafficking protein 7 [Source:ZFIN;Acc:ZDB-GENE-040426-2570])

HSP 1 Score: 59.3066 bits (142), Expect = 1.616e-10
Identity = 43/202 (21.29%), Postives = 85/202 (42.08%), Query Frame = 1
Query:   34 ALFVHIII----INNLSSFEINLNPNSEECLFDYFNKSDKVEFSYEVINGGFLDIESKLLSPMKVEFMRRDKGSYGVYDLVIEQSGLYYFCLINKEGSQVEKTVRFNLF---SEPHYRWERNITSRESKNSDLEEIVHDLSFLMHSVKAESEYSLTREQIHDQANRSANRHFLLWGLFEMVLMVAMSLGQIAYLKHLFEVRR 618
             LFV +++    +++ S     L  N+++C ++      K    ++V+ GG  D++ +L  P      +  K  Y  +     ++G Y FC  N+  +   KTV F+       P +  E  +T+     S    I   L  +M     ++ + L   Q   +A    N     W + E ++++ +S+ Q+  L+  F  R+
Sbjct:   11 PLFVQVVMMKVGLSSASELTFELPDNAKQCFYEDITIGTKCTLEFQVVTGGHYDVDCRLEDPEGTVLYKEMKKQYDSFTFSAARNGTYKFCFSNEFSTFTHKTVYFDFQVGDDPPLFPNENRVTALTQMESACVSIHEALKSVM---DYQTHFRLREAQGRSRAE-DLNSRVAFWSVGEALILLVVSVSQVLLLRSFFSDRK 208          
BLAST of transmembrane emp24 domain-containing protein 2-like vs. Ensembl Zebrafish
Match: tmed7 (transmembrane p24 trafficking protein 7 [Source:ZFIN;Acc:ZDB-GENE-040426-2570])

HSP 1 Score: 59.3066 bits (142), Expect = 1.616e-10
Identity = 43/202 (21.29%), Postives = 85/202 (42.08%), Query Frame = 1
Query:   34 ALFVHIII----INNLSSFEINLNPNSEECLFDYFNKSDKVEFSYEVINGGFLDIESKLLSPMKVEFMRRDKGSYGVYDLVIEQSGLYYFCLINKEGSQVEKTVRFNLF---SEPHYRWERNITSRESKNSDLEEIVHDLSFLMHSVKAESEYSLTREQIHDQANRSANRHFLLWGLFEMVLMVAMSLGQIAYLKHLFEVRR 618
             LFV +++    +++ S     L  N+++C ++      K    ++V+ GG  D++ +L  P      +  K  Y  +     ++G Y FC  N+  +   KTV F+       P +  E  +T+     S    I   L  +M     ++ + L   Q   +A    N     W + E ++++ +S+ Q+  L+  F  R+
Sbjct:   11 PLFVQVVMMKVGLSSASELTFELPDNAKQCFYEDITIGTKCTLEFQVVTGGHYDVDCRLEDPEGTVLYKEMKKQYDSFTFSAARNGTYKFCFSNEFSTFTHKTVYFDFQVGDDPPLFPNENRVTALTQMESACVSIHEALKSVM---DYQTHFRLREAQGRSRAE-DLNSRVAFWSVGEALILLVVSVSQVLLLRSFFSDRK 208          
BLAST of transmembrane emp24 domain-containing protein 2-like vs. Ensembl Zebrafish
Match: tmed7 (transmembrane p24 trafficking protein 7 [Source:ZFIN;Acc:ZDB-GENE-040426-2570])

HSP 1 Score: 59.3066 bits (142), Expect = 1.616e-10
Identity = 43/202 (21.29%), Postives = 85/202 (42.08%), Query Frame = 1
Query:   34 ALFVHIII----INNLSSFEINLNPNSEECLFDYFNKSDKVEFSYEVINGGFLDIESKLLSPMKVEFMRRDKGSYGVYDLVIEQSGLYYFCLINKEGSQVEKTVRFNLF---SEPHYRWERNITSRESKNSDLEEIVHDLSFLMHSVKAESEYSLTREQIHDQANRSANRHFLLWGLFEMVLMVAMSLGQIAYLKHLFEVRR 618
             LFV +++    +++ S     L  N+++C ++      K    ++V+ GG  D++ +L  P      +  K  Y  +     ++G Y FC  N+  +   KTV F+       P +  E  +T+     S    I   L  +M     ++ + L   Q   +A    N     W + E ++++ +S+ Q+  L+  F  R+
Sbjct:   11 PLFVQVVMMKVGLSSASELTFELPDNAKQCFYEDITIGTKCTLEFQVVTGGHYDVDCRLEDPEGTVLYKEMKKQYDSFTFSAARNGTYKFCFSNEFSTFTHKTVYFDFQVGDDPPLFPNENRVTALTQMESACVSIHEALKSVM---DYQTHFRLREAQGRSRAE-DLNSRVAFWSVGEALILLVVSVSQVLLLRSFFSDRK 208          
BLAST of transmembrane emp24 domain-containing protein 2-like vs. Ensembl Xenopus
Match: tmed2 (transmembrane p24 trafficking protein 2 [Source:NCBI gene;Acc:734103])

HSP 1 Score: 121.324 bits (303), Expect = 6.611e-34
Identity = 65/185 (35.14%), Postives = 100/185 (54.05%), Query Frame = 1
Query:   70 SSFEINLNPNSEECLFDYFNKSDKVEFSYEVINGGFLDIESKLLSPMKVEFMRRDKGSYGVYDLVIEQSGLYYFCLINKEGSQVEKTVRFNLFSEPHYRWERNITSRESKNSDLEEIVHDLSFLMHSVKAESEYSLTREQIHDQANRSANRHFLLWGLFEMVLMVAMSLGQIAYLKHLFEVRRFV 624
            S + ++++ ++EEC F+      K+   +EV  GGFLDI+ ++  P      + D+ S G Y       G Y FC  N+  +   K V F +      + +   T  E+  + LEE++++L+  M +VK E EY   RE+IH   N + N   +LW  FE +++VAM+LGQI YLK  FEVRR V
Sbjct:   19 SGYFVSIDAHAEECFFEQVTSGTKMGLIFEVAEGGFLDIDVEITGPDNKGIHKGDRESSGKYTFAAHMDGTYKFCFSNRMSTMTPKIVMFTIDIGEAPKGQDMET--EAHQNKLEEMINELAVAMTAVKHEQEYMEVRERIHRAINDNTNSRVVLWSFFEALVLVAMTLGQIYYLKRFFEVRRVV 201          
BLAST of transmembrane emp24 domain-containing protein 2-like vs. Ensembl Xenopus
Match: tmed2 (transmembrane p24 trafficking protein 2 [Source:NCBI gene;Acc:734103])

HSP 1 Score: 119.398 bits (298), Expect = 5.610e-33
Identity = 65/190 (34.21%), Postives = 101/190 (53.16%), Query Frame = 1
Query:   70 SSFEINLNPNSEECLFDYFNKSDKVEFSYEVINGGFLDIESKLLSPMKVEFMRRDKGSYGVYDLVIEQSGLYYFCLINKEGSQVEKTVRFNLFSEPHYRWERNIT-----SRESKNSDLEEIVHDLSFLMHSVKAESEYSLTREQIHDQANRSANRHFLLWGLFEMVLMVAMSLGQIAYLKHLFEVRRFV 624
            S + ++++ ++EEC F+      K+   +EV  GGFLDI+ ++  P      + D+ S G Y       G Y FC  N+  +   K V F +      + +   T     S ++  + LEE++++L+  M +VK E EY   RE+IH   N + N   +LW  FE +++VAM+LGQI YLK  FEVRR V
Sbjct:   19 SGYFVSIDAHAEECFFEQVTSGTKMGLIFEVAEGGFLDIDVEITGPDNKGIHKGDRESSGKYTFAAHMDGTYKFCFSNRMSTMTPKIVMFTIDIGEAPKGQDMETEGIGESWDAHQNKLEEMINELAVAMTAVKHEQEYMEVRERIHRAINDNTNSRVVLWSFFEALVLVAMTLGQIYYLKRFFEVRRVV 208          
BLAST of transmembrane emp24 domain-containing protein 2-like vs. Ensembl Xenopus
Match: serinc2 (serine incorporator 2 [Source:Xenbase;Acc:XB-GENE-968901])

HSP 1 Score: 62.7734 bits (151), Expect = 1.669e-11
Identity = 48/206 (23.30%), Postives = 89/206 (43.20%), Query Frame = 1
Query:   25 LHFALFVHIIIINNLSSFEINLNPNSEECLFDYFNKSDKVEFSYEVINGGFLDIESKLLSPMKVEFMRRDKGSYGVYDLVIEQSGLYYFCLINKEGSQVEKTVRFN--------LFSEPHYRWERNITSRESKNSDLEEIVHDLSFLMHSVKAESEYSLTREQIHDQANRSANRHFLLWGLFEMVLMVAMSLGQIAYLKHLFEVRR 618
            L F LF    +  +  +FE  L  N+++C ++   +  K    ++VI GG  D++ +L  P  +   +  K  Y  +     ++G Y FC  N+  +   KTV F+        LF  P+      +T  ES    + E +  +      +  ++ + L   Q   +A    N     W + E ++++ +S+GQ+  LK  F  +R
Sbjct:   17 LSFVLFSLGCVRASELTFE--LPDNAKQCFYEDITQGTKCTLEFQVITGGHYDVDCRLEDPDGIVLYKEMKKQYDSFTFTATRNGTYKFCFSNEFSTFTHKTVYFDFQVGDDPPLF--PNENRATALTQMESSCVSIHEALKSV------IDYQTHFRLREAQGRSRAE-DLNSRVAYWSIGEAIILLVVSIGQVFLLKSFFSDKR 211          
BLAST of transmembrane emp24 domain-containing protein 2-like vs. Ensembl Xenopus
Match: serinc2 (serine incorporator 2 [Source:Xenbase;Acc:XB-GENE-968901])

HSP 1 Score: 62.3882 bits (150), Expect = 2.008e-11
Identity = 48/206 (23.30%), Postives = 89/206 (43.20%), Query Frame = 1
Query:   25 LHFALFVHIIIINNLSSFEINLNPNSEECLFDYFNKSDKVEFSYEVINGGFLDIESKLLSPMKVEFMRRDKGSYGVYDLVIEQSGLYYFCLINKEGSQVEKTVRFN--------LFSEPHYRWERNITSRESKNSDLEEIVHDLSFLMHSVKAESEYSLTREQIHDQANRSANRHFLLWGLFEMVLMVAMSLGQIAYLKHLFEVRR 618
            L F LF    +  +  +FE  L  N+++C ++   +  K    ++VI GG  D++ +L  P  +   +  K  Y  +     ++G Y FC  N+  +   KTV F+        LF  P+      +T  ES    + E +  +      +  ++ + L   Q   +A    N     W + E ++++ +S+GQ+  LK  F  +R
Sbjct:   23 LSFVLFSLGCVRASELTFE--LPDNAKQCFYEDITQGTKCTLEFQVITGGHYDVDCRLEDPDGIVLYKEMKKQYDSFTFTATRNGTYKFCFSNEFSTFTHKTVYFDFQVGDDPPLF--PNENRATALTQMESSCVSIHEALKSV------IDYQTHFRLREAQGRSRAE-DLNSRVAYWSIGEAIILLVVSIGQVFLLKSFFSDKR 217          
BLAST of transmembrane emp24 domain-containing protein 2-like vs. Ensembl Xenopus
Match: tmed5 (transmembrane p24 trafficking protein 5 [Source:NCBI gene;Acc:549314])

HSP 1 Score: 58.5362 bits (140), Expect = 4.704e-10
Identity = 52/198 (26.26%), Postives = 87/198 (43.94%), Query Frame = 1
Query:   70 SSFEINLNPNSEECLFDYFNKSDKVEFSYEVINGGFLDIESKLLSPMKVEFMRRDKGSYGVYDLVIEQSGLYYFCLINKEGSQVEKTVRFNLFSEPHYR--------WERNITSRESKNSDLEEIVHDLSFLMHSVK------AESEYSLTREQIHDQANRSAN-RHFLLWGLFEMVLMVAMSLGQIAYLKHLFEVRR 618
            S +   L    +EC F    +   +E  Y+V++G  LD++  L SP     +  ++ S GV+  V    G Y FC  N      EK + F L  + H          W+  IT  +  +  LE+I+  +    +SVK      A+ +  L   +  D+  + +N      W +F + +MV +S  Q+  L+ LFE +R
Sbjct:   28 SDYTFTLPAGQKECFFQPMKRDATLEIEYQVLDGAGLDVDFSLTSPNGELLLSEERQSDGVHS-VETIDGDYQFCFDNSFSRMSEKVIFFELILD-HLNDEGNELEDWKSYITGTDLLDMKLEDILETI----NSVKGRLTKSAQIQTLLKAFEARDRNLQESNFERVTFWSVFNLTVMVVVSALQVYMLRSLFEDKR 219          
BLAST of transmembrane emp24 domain-containing protein 2-like vs. Ensembl Mouse
Match: Tmed2 (transmembrane p24 trafficking protein 2 [Source:MGI Symbol;Acc:MGI:1929269])

HSP 1 Score: 121.324 bits (303), Expect = 4.953e-34
Identity = 65/185 (35.14%), Postives = 100/185 (54.05%), Query Frame = 1
Query:   70 SSFEINLNPNSEECLFDYFNKSDKVEFSYEVINGGFLDIESKLLSPMKVEFMRRDKGSYGVYDLVIEQSGLYYFCLINKEGSQVEKTVRFNLFSEPHYRWERNITSRESKNSDLEEIVHDLSFLMHSVKAESEYSLTREQIHDQANRSANRHFLLWGLFEMVLMVAMSLGQIAYLKHLFEVRRFV 624
            S + ++++ ++EEC F+      K+   +EV  GGFLDI+ ++  P      + D+ S G Y       G Y FC  N+  +   K V F +      + +   T  E+  + LEE++++L+  M +VK E EY   RE+IH   N + N   +LW  FE +++VAM+LGQI YLK  FEVRR V
Sbjct:   19 SGYFVSIDAHAEECFFERVTSGTKMGLIFEVAEGGFLDIDVEITGPDNKGIYKGDRESSGKYTFAAHMDGTYKFCFSNRMSTMTPKIVMFTIDIGEAPKGQDMET--EAHQNKLEEMINELAVAMTAVKHEQEYMEVRERIHRAINDNTNSRVVLWSFFEALVLVAMTLGQIYYLKRFFEVRRVV 201          
BLAST of transmembrane emp24 domain-containing protein 2-like vs. Ensembl Mouse
Match: Tmed2 (transmembrane p24 trafficking protein 2 [Source:MGI Symbol;Acc:MGI:1929269])

HSP 1 Score: 90.1225 bits (222), Expect = 2.766e-22
Identity = 51/144 (35.42%), Postives = 75/144 (52.08%), Query Frame = 1
Query:  193 KLLSPMKVEFMRRDKGSYGVYDLVIEQSGLYYFCLINKEGSQVEKTVRFNLFSEPHYRWERNITSRESKNSDLEEIVHDLSFLMHSVKAESEYSLTREQIHDQANRSANRHFLLWGLFEMVLMVAMSLGQIAYLKHLFEVRRFV 624
            ++  P      + D+ S G Y       G Y FC  N+  +   K V F +      + +   T  E+  + LEE++++L+  M +VK E EY   RE+IH   N + N   +LW  FE +++VAM+LGQI YLK  FEVRR V
Sbjct:   35 RITGPDNKGIYKGDRESSGKYTFAAHMDGTYKFCFSNRMSTMTPKIVMFTIDIGEAPKGQDMET--EAHQNKLEEMINELAVAMTAVKHEQEYMEVRERIHRAINDNTNSRVVLWSFFEALVLVAMTLGQIYYLKRFFEVRRVV 176          
BLAST of transmembrane emp24 domain-containing protein 2-like vs. Ensembl Mouse
Match: Tmed2 (transmembrane p24 trafficking protein 2 [Source:MGI Symbol;Acc:MGI:1929269])

HSP 1 Score: 75.8702 bits (185), Expect = 1.867e-17
Identity = 37/80 (46.25%), Postives = 53/80 (66.25%), Query Frame = 1
Query:  385 SRESKNSDLEEIVHDLSFLMHSVKAESEYSLTREQIHDQANRSANRHFLLWGLFEMVLMVAMSLGQIAYLKHLFEVRRFV 624
            S ++  + LEE++++L+  M +VK E EY   RE+IH   N + N   +LW  FE +++VAM+LGQI YLK  FEVRR V
Sbjct:   33 SWDAHQNKLEEMINELAVAMTAVKHEQEYMEVRERIHRAINDNTNSRVVLWSFFEALVLVAMTLGQIYYLKRFFEVRRVV 112          
BLAST of transmembrane emp24 domain-containing protein 2-like vs. Ensembl Mouse
Match: Tmed7 (transmembrane p24 trafficking protein 7 [Source:MGI Symbol;Acc:MGI:1913926])

HSP 1 Score: 57.7658 bits (138), Expect = 6.837e-10
Identity = 42/186 (22.58%), Postives = 79/186 (42.47%), Query Frame = 1
Query:   70 SSFEINLNPNSEECLFDYFNKSDKVEFSYEVINGGFLDIESKLLSPMKVEFMRRDKGSYGVYDLVIEQSGLYYFCLINKEGSQVEKTVRFNLF---SEPHYRWERNITSRESKNSDLEEIVHDLSFLMHSVKAESEYSLTREQIHDQANRSANRHFLLWGLFEMVLMVAMSLGQIAYLKHLFEVRR 618
            S     L  N+++C ++   +  K    ++VI GG  D++ +L  P      +  K  Y  +     ++G Y FC  N+  +   KTV F+       P +  E  +++     S    I H+   L   +  ++ + L   Q   +A    N     W + E ++++ +S+GQ+  LK  F  +R
Sbjct:   35 SEITFELPDNAKQCFYEDITQGTKCTLEFQVITGGHYDVDCRLEDPDGKVLYKEMKKQYDSFTFTASRNGTYKFCFSNEFSTFTHKTVYFDFQVGEDPPLFPSENRVSALTQMESACVSI-HEA--LKSVIDYQTHFRLREAQGRSRAE-DLNTRVAYWSVGEALILLVVSVGQVFLLKSFFSDKR 216          
BLAST of transmembrane emp24 domain-containing protein 2-like vs. Ensembl Mouse
Match: Tmed3 (transmembrane p24 trafficking protein 3 [Source:MGI Symbol;Acc:MGI:1913361])

HSP 1 Score: 52.7582 bits (125), Expect = 4.449e-8
Identity = 42/182 (23.08%), Postives = 75/182 (41.21%), Query Frame = 1
Query:   88 LNPNSEECLFDYFNKSDKVEFSYEVINGGFLDIESKLLSPMKVEFMRRDKGSYGVYDLVIEQSGLYYFCLINKEGSQVEKTVRFNLFSEPHYRWERNITSRESKNSDLEE---IVHDLSFLMHSVKAESEYSLTREQIHDQANRSANRHFLLWGLFEMVLMVAMSLGQIAYLKHLFEVRRFV 624
            L  N+++C  +   +  K    Y+VI GG  D++  +  P      R  K  Y  +    E  G+Y FC  N+  +   KTV F+           ++ +R +  + +E     +H+   L   + +++ Y L   Q   +A    N     W + E + +  +S  Q+  LK  F  +R V
Sbjct:   37 LPDNAKQCFHEEVEQGVKFSLDYQVITGGHYDVDCYVEDPRGNVIYRETKKQYDSFTYKTEAKGVYRFCFSNEFSTFSHKTVYFDFQVGDEPPILPDMGNRVTALTQMESACVTIHEA--LKTVIDSQTHYRLREAQDRARAE-DLNSRVSYWSVGETIALFVVSFSQVLLLKSFFTEKRPV 215          
BLAST of transmembrane emp24 domain-containing protein 2-like vs. UniProt/SwissProt
Match: sp|O17528|TMED2_CAEEL (Suppressor/enhancer of lin-12 protein 9 OS=Caenorhabditis elegans OX=6239 GN=sel-9 PE=1 SV=1)

HSP 1 Score: 125.561 bits (314), Expect = 1.087e-34
Identity = 71/205 (34.63%), Postives = 108/205 (52.68%), Query Frame = 1
Query:   16 MNKLHFALFVHIIIINNLSSFEINLNPNSEECLFDYFNKSDKVEFSYEVINGGFLDIESKLLSPMKVEFMRRDKGSYGVYDLVIEQSGLYYFCLINKEGSQVEKTVRFNL-FSEPHYRWE-RNITSRESKNSDLEEIVHDLSFLMHSVKAESEYSLTREQIHDQANRSANRHFLLWGLFEMVLMVAMSLGQIAYLKHLFEVRRFV 624
            MN L + L V  + +   +S+ I+++ N E+C FD      K+   +EV  GGFLDI+ K+  P   E  + ++ S G +       G+Y +C  NK  +   K V F +  +EPH +          + N+ LEE+V +LS  + SVK E EY   RE++H   N + N   ++W  FE  ++V M++GQI YLK  FEVR  V
Sbjct:    1 MNSLTWILAV--LFVTPAASYFIHVDANEEQCFFDRLTSGTKMGLMFEVAEGGFLDIDVKITGPDNKEIYKGERESSGKFTFAAHMDGVYTYCFGNKMSTMTPKAVMFTVEITEPHQQAPGAAANQDAADNAKLEEMVRELSSALMSVKHEQEYMEVRERVHRNINENTNSRVVMWAAFEAFVLVGMTVGQIFYLKRFFEVRTMV 203          
BLAST of transmembrane emp24 domain-containing protein 2-like vs. UniProt/SwissProt
Match: sp|P49020|TMED2_CRIGR (Transmembrane emp24 domain-containing protein 2 (Fragment) OS=Cricetulus griseus OX=10029 GN=TMED2 PE=1 SV=1)

HSP 1 Score: 121.324 bits (303), Expect = 3.130e-33
Identity = 65/185 (35.14%), Postives = 100/185 (54.05%), Query Frame = 1
Query:   70 SSFEINLNPNSEECLFDYFNKSDKVEFSYEVINGGFLDIESKLLSPMKVEFMRRDKGSYGVYDLVIEQSGLYYFCLINKEGSQVEKTVRFNLFSEPHYRWERNITSRESKNSDLEEIVHDLSFLMHSVKAESEYSLTREQIHDQANRSANRHFLLWGLFEMVLMVAMSLGQIAYLKHLFEVRRFV 624
            S + ++++ ++EEC F+      K+   +EV  GGFLDI+ ++  P      + D+ S G Y       G Y FC  N+  +   K V F +      + +   T  E+  + LEE++++L+  M +VK E EY   RE+IH   N + N   +LW  FE +++VAM+LGQI YLK  FEVRR V
Sbjct:   14 SGYFVSIDAHAEECFFERVTSGTKMGLIFEVAEGGFLDIDVEITGPDNKGIYKGDRESSGKYTFAAHMDGTYKFCFSNRMSTMTPKIVMFTIDIGEAPKGQDMET--EAHQNKLEEMINELAVAMTAVKHEQEYMEVRERIHRAINDNTNSRVVLWSFFEALVLVAMTLGQIYYLKRFFEVRRVV 196          
BLAST of transmembrane emp24 domain-containing protein 2-like vs. UniProt/SwissProt
Match: sp|Q9R0Q3|TMED2_MOUSE (Transmembrane emp24 domain-containing protein 2 OS=Mus musculus OX=10090 GN=Tmed2 PE=1 SV=1)

HSP 1 Score: 121.324 bits (303), Expect = 3.465e-33
Identity = 65/185 (35.14%), Postives = 100/185 (54.05%), Query Frame = 1
Query:   70 SSFEINLNPNSEECLFDYFNKSDKVEFSYEVINGGFLDIESKLLSPMKVEFMRRDKGSYGVYDLVIEQSGLYYFCLINKEGSQVEKTVRFNLFSEPHYRWERNITSRESKNSDLEEIVHDLSFLMHSVKAESEYSLTREQIHDQANRSANRHFLLWGLFEMVLMVAMSLGQIAYLKHLFEVRRFV 624
            S + ++++ ++EEC F+      K+   +EV  GGFLDI+ ++  P      + D+ S G Y       G Y FC  N+  +   K V F +      + +   T  E+  + LEE++++L+  M +VK E EY   RE+IH   N + N   +LW  FE +++VAM+LGQI YLK  FEVRR V
Sbjct:   19 SGYFVSIDAHAEECFFERVTSGTKMGLIFEVAEGGFLDIDVEITGPDNKGIYKGDRESSGKYTFAAHMDGTYKFCFSNRMSTMTPKIVMFTIDIGEAPKGQDMET--EAHQNKLEEMINELAVAMTAVKHEQEYMEVRERIHRAINDNTNSRVVLWSFFEALVLVAMTLGQIYYLKRFFEVRRVV 201          
BLAST of transmembrane emp24 domain-containing protein 2-like vs. UniProt/SwissProt
Match: sp|Q63524|TMED2_RAT (Transmembrane emp24 domain-containing protein 2 OS=Rattus norvegicus OX=10116 GN=Tmed2 PE=1 SV=1)

HSP 1 Score: 121.324 bits (303), Expect = 3.656e-33
Identity = 65/185 (35.14%), Postives = 100/185 (54.05%), Query Frame = 1
Query:   70 SSFEINLNPNSEECLFDYFNKSDKVEFSYEVINGGFLDIESKLLSPMKVEFMRRDKGSYGVYDLVIEQSGLYYFCLINKEGSQVEKTVRFNLFSEPHYRWERNITSRESKNSDLEEIVHDLSFLMHSVKAESEYSLTREQIHDQANRSANRHFLLWGLFEMVLMVAMSLGQIAYLKHLFEVRRFV 624
            S + ++++ ++EEC F+      K+   +EV  GGFLDI+ ++  P      + D+ S G Y       G Y FC  N+  +   K V F +      + +   T  E+  + LEE++++L+  M +VK E EY   RE+IH   N + N   +LW  FE +++VAM+LGQI YLK  FEVRR V
Sbjct:   19 SGYFVSIDAHAEECFFERVTSGTKMGLIFEVAEGGFLDIDVEITGPDNKGIYKGDRESSGKYTFAAHMDGTYKFCFSNRMSTMTPKIVMFTIDIGEAPKGQDMET--EAHQNKLEEMINELAVAMTAVKHEQEYMEVRERIHRAINDNTNSRVVLWSFFEALVLVAMTLGQIYYLKRFFEVRRVV 201          
BLAST of transmembrane emp24 domain-containing protein 2-like vs. UniProt/SwissProt
Match: sp|Q15363|TMED2_HUMAN (Transmembrane emp24 domain-containing protein 2 OS=Homo sapiens OX=9606 GN=TMED2 PE=1 SV=1)

HSP 1 Score: 121.324 bits (303), Expect = 3.656e-33
Identity = 65/185 (35.14%), Postives = 100/185 (54.05%), Query Frame = 1
Query:   70 SSFEINLNPNSEECLFDYFNKSDKVEFSYEVINGGFLDIESKLLSPMKVEFMRRDKGSYGVYDLVIEQSGLYYFCLINKEGSQVEKTVRFNLFSEPHYRWERNITSRESKNSDLEEIVHDLSFLMHSVKAESEYSLTREQIHDQANRSANRHFLLWGLFEMVLMVAMSLGQIAYLKHLFEVRRFV 624
            S + ++++ ++EEC F+      K+   +EV  GGFLDI+ ++  P      + D+ S G Y       G Y FC  N+  +   K V F +      + +   T  E+  + LEE++++L+  M +VK E EY   RE+IH   N + N   +LW  FE +++VAM+LGQI YLK  FEVRR V
Sbjct:   19 SGYFVSIDAHAEECFFERVTSGTKMGLIFEVAEGGFLDIDVEITGPDNKGIYKGDRESSGKYTFAAHMDGTYKFCFSNRMSTMTPKIVMFTIDIGEAPKGQDMET--EAHQNKLEEMINELAVAMTAVKHEQEYMEVRERIHRAINDNTNSRVVLWSFFEALVLVAMTLGQIYYLKRFFEVRRVV 201          
BLAST of transmembrane emp24 domain-containing protein 2-like vs. TrEMBL
Match: A0A1W4XF97 (transmembrane emp24 domain-containing protein 2-like OS=Agrilus planipennis OX=224129 GN=LOC108743600 PE=3 SV=1)

HSP 1 Score: 138.658 bits (348), Expect = 2.336e-37
Identity = 71/200 (35.50%), Postives = 110/200 (55.00%), Query Frame = 1
Query:   25 LHFALFVHIIIINNLSSFEINLNPNSEECLFDYFNKSDKVEFSYEVINGGFLDIESKLLSPMKVEFMRRDKGSYGVYDLVIEQSGLYYFCLINKEGSQVEKTVRFNLFSEPHYRWERNITSRESKNSDLEEIVHDLSFLMHSVKAESEYSLTREQIHDQANRSANRHFLLWGLFEMVLMVAMSLGQIAYLKHLFEVRRFV 624
            L   LF +  IIN++S + I ++ ++EEC FD      K+  ++E+  GGFLDI+ +++ P      + ++ S G Y      +G+Y +C  NK  +   K V FN+      + E N    E  ++ LE+++ +LS  +  VK E EY   R++IH   N S N   ++W  FE V++VAM+ GQI YLK  FEVRR V
Sbjct:    5 LLIVLFAYSSIINSVSCYYITVDADAEECFFDKVESGTKMSLTFEIAEGGFLDIDVRIVGPDGKVIYQGERESSGKYTFAAHANGIYTYCFSNKMSTMTPKVVMFNMQIGESAKHEEN--QGEESSTKLEDMIRELSGALTGVKQEQEYMQLRDRIHRSINESTNSRVVMWSFFEAVILVAMTFGQIYYLKRFFEVRRVV 202          
BLAST of transmembrane emp24 domain-containing protein 2-like vs. TrEMBL
Match: A8XDC7 (Protein CBR-SEL-9 OS=Caenorhabditis briggsae OX=6238 GN=sel-9 PE=3 SV=1)

HSP 1 Score: 136.732 bits (343), Expect = 1.443e-36
Identity = 71/205 (34.63%), Postives = 113/205 (55.12%), Query Frame = 1
Query:   16 MNKLHFALFVHIIIINNLSSFEINLNPNSEECLFDYFNKSDKVEFSYEVINGGFLDIESKLLSPMKVEFMRRDKGSYGVYDLVIEQSGLYYFCLINKEGSQVEKTVRFNL-FSEPHYRWERNITSRESK-NSDLEEIVHDLSFLMHSVKAESEYSLTREQIHDQANRSANRHFLLWGLFEMVLMVAMSLGQIAYLKHLFEVRRFV 624
            MN + +AL V  +++   +S+ I+++ N E+C FD      K+   +EV  GGFLDI+ K+  P   E  + ++ S G +       G+Y +C  NK  +   K V F +  +EPH +      S+++  N+ LEE+V +LS  + SVK E EY   RE++H   N + N   ++W  FE  ++V M++GQ+ YLK  FEVR  V
Sbjct:    1 MNSITWALAV--LLVTPAASYFIHVDANEEQCFFDRLTSGTKMGLMFEVAEGGFLDIDVKITGPDNKEIYKGERESSGKFTFAAHMDGIYTYCFGNKMSTMTPKAVMFTVEITEPHQQAPGAAASQDAADNAKLEEMVRELSSALMSVKHEQEYMEVRERVHRNINENTNSRVVMWAAFEAFVLVGMTIGQVFYLKRFFEVRTMV 203          
BLAST of transmembrane emp24 domain-containing protein 2-like vs. TrEMBL
Match: A0A2H2IU33 (GOLD domain-containing protein OS=Caenorhabditis japonica OX=281687 PE=3 SV=1)

HSP 1 Score: 136.732 bits (343), Expect = 1.491e-36
Identity = 71/200 (35.50%), Postives = 109/200 (54.50%), Query Frame = 1
Query:   31 FALFVHIIIINNLSSFEINLNPNSEECLFDYFNKSDKVEFSYEVINGGFLDIESKLLSPMKVEFMRRDKGSYGVYDLVIEQSGLYYFCLINKEGSQVEKTVRFNL-FSEPHYRWERNITSRESK-NSDLEEIVHDLSFLMHSVKAESEYSLTREQIHDQANRSANRHFLLWGLFEMVLMVAMSLGQIAYLKHLFEVRRFV 624
            F   + I+++   +S+ I+++ N E+C FD      K+   +EV  GGFLDI+ K+  P   E  + ++ S G +       G+Y +C  NK  +   K V F +  +EPH       TS+++  N+ LEE+V +LS  + SVK E EY   RE++H   N + N   +LW  FE  ++V M++GQI YLK  FEVR  V
Sbjct:    4 FTWILSILLVTPAASYFIHVDANEEQCFFDRLTSGTKMGLMFEVAEGGFLDIDVKITGPDNKEIYKGERESSGKFTFSAHMDGIYTYCFGNKMSTMTPKAVMFTVEVTEPHQSAPGAATSQDAADNAKLEEMVRELSSALMSVKHEQEYMEVRERVHRNINENTNSRVVLWAAFEAFVLVGMTVGQIFYLKRFFEVRTMV 203          
BLAST of transmembrane emp24 domain-containing protein 2-like vs. TrEMBL
Match: G0NHK8 (CBN-SEL-9 protein OS=Caenorhabditis brenneri OX=135651 GN=Cbn-sel-9 PE=3 SV=1)

HSP 1 Score: 134.035 bits (336), Expect = 1.544e-35
Identity = 72/205 (35.12%), Postives = 112/205 (54.63%), Query Frame = 1
Query:   16 MNKLHFALFVHIIIINNLSSFEINLNPNSEECLFDYFNKSDKVEFSYEVINGGFLDIESKLLSPMKVEFMRRDKGSYGVYDLVIEQSGLYYFCLINKEGSQVEKTVRFNL-FSEPHYRWERNITSRESK-NSDLEEIVHDLSFLMHSVKAESEYSLTREQIHDQANRSANRHFLLWGLFEMVLMVAMSLGQIAYLKHLFEVRRFV 624
            MN L + L + ++ I   +S+ I+++ N E+C FD      K+   +EV  GGFLDI+ K+  P   E  + ++ S G +       G+Y +C  NK  +   K V F +  +EPH +      S+++  N+ LEE+V +LS  + SVK E EY   RE++H   N + N   ++W  FE  ++V M++GQI YLK  FEVR  V
Sbjct:    1 MNSLTWILAI-LLSITPAASYFIHVDANEEQCFFDRLTSGTKMGLMFEVAEGGFLDIDVKITGPDNKEIYKGERESSGKFTFAAHMDGVYTYCFGNKMSTMTPKAVMFTVEITEPHAQAPGAPVSQDAADNAKLEEMVRELSSALMSVKHEQEYMEVRERVHRNINENTNSRVVMWAAFEAFVLVGMTVGQIFYLKRFFEVRTMV 204          
BLAST of transmembrane emp24 domain-containing protein 2-like vs. TrEMBL
Match: A0A2G5T5U9 (GOLD domain-containing protein OS=Caenorhabditis nigoni OX=1611254 GN=Cni-sel-9 PE=3 SV=1)

HSP 1 Score: 134.035 bits (336), Expect = 1.690e-35
Identity = 66/194 (34.02%), Postives = 107/194 (55.15%), Query Frame = 1
Query:   49 IIIINNLSSFEINLNPNSEECLFDYFNKSDKVEFSYEVINGGFLDIESKLLSPMKVEFMRRDKGSYGVYDLVIEQSGLYYFCLINKEGSQVEKTVRFNL-FSEPHYRWERNITSRESK-NSDLEEIVHDLSFLMHSVKAESEYSLTREQIHDQANRSANRHFLLWGLFEMVLMVAMSLGQIAYLKHLFEVRRFV 624
            ++++   +S+ I+++ N E+C FD      K+   +EV  GGFLDI+ K+  P   E  + ++ S G +       G+Y +C  NK  +   K V F +  +EPH +      S+++  N+ LEE+V +LS  + SVK E EY   RE++H   N + N   ++W  FE  ++V M++GQ+ YLK  FEVR  V
Sbjct:   10 VLLVTPAASYFIHVDANEEQCFFDRLTSGTKMGLMFEVAEGGFLDIDVKITGPDNKEIYKGERESSGKFTFAAHMDGIYTYCFGNKMSTMTPKAVMFTVEITEPHQQAPGAAASQDAADNAKLEEMVRELSSALMSVKHEQEYMEVRERVHRNINENTNSRVVMWAAFEAFVLVGMTIGQVFYLKRFFEVRTMV 203          
BLAST of transmembrane emp24 domain-containing protein 2-like vs. Ensembl Cavefish
Match: tmed2 (transmembrane emp24 domain-containing protein 2 [Source:NCBI gene;Acc:111194998])

HSP 1 Score: 124.02 bits (310), Expect = 4.225e-35
Identity = 65/181 (35.91%), Postives = 101/181 (55.80%), Query Frame = 1
Query:   82 INLNPNSEECLFDYFNKSDKVEFSYEVINGGFLDIESKLLSPMKVEFMRRDKGSYGVYDLVIEQSGLYYFCLINKEGSQVEKTVRFNLFSEPHYRWERNITSRESKNSDLEEIVHDLSFLMHSVKAESEYSLTREQIHDQANRSANRHFLLWGLFEMVLMVAMSLGQIAYLKHLFEVRRFV 624
            ++++ ++EEC ++  N   K+   +EV  GGFLDI+ ++  P   +  + D+ S G Y +     G Y FC  NK  +   K V F +      + +   T  E+  + LEE++++L+  M +VK E EY   RE+IH   N + N   +LW  FE +++VAM+LGQI YLK  FEVRR V
Sbjct:   24 VSVDAHAEECFYERVNSGTKMGLMFEVAEGGFLDIDVEITGPDGKQIYKGDRESSGKYSVAAHMDGTYKFCFSNKMSTMTPKIVMFTIDIGEAPKGQDMET--EAHQNKLEEMINELAVAMTAVKHEQEYMEVRERIHRAINDNTNSRVVLWSFFEALVLVAMTLGQIYYLKRFFEVRRVV 202          
BLAST of transmembrane emp24 domain-containing protein 2-like vs. Ensembl Cavefish
Match: TMED2 (transmembrane emp24 domain-containing protein 2 [Source:NCBI gene;Acc:103022411])

HSP 1 Score: 123.635 bits (309), Expect = 7.873e-35
Identity = 66/190 (34.74%), Postives = 104/190 (54.74%), Query Frame = 1
Query:   70 SSFEINLNPNSEECLFDYFNKSDKVEFSYEVINGGFLDIESKLLSPMKVEFMRRDKGSYGVYDLVIEQSGLYYFCLINKEGSQVEKTVRF--NLFSEPH---YRWERNITSRESKNSDLEEIVHDLSFLMHSVKAESEYSLTREQIHDQANRSANRHFLLWGLFEMVLMVAMSLGQIAYLKHLFEVRRFV 624
            S + ++++ ++EEC ++  N   K+   +EV  GGFLDI+ ++  P   +  + D+ S G Y +     G Y FC  NK  +   K V F  ++   P       E    + ++  + LEE++++L+  M +VK E EY   RE+IH   N + N   +LW  FE +++VAM+LGQI YLK  FEVRR V
Sbjct:   19 SGYFVSVDAHAEECFYERVNSGTKMGLMFEVAEGGFLDIDVEITGPDGKQIYKGDRESSGKYSVAAHMDGTYKFCFSNKMSTMTPKIVMFTIDIGEAPKGDGMETEVGGGTWDAHQNKLEEMINELAVAMTAVKHEQEYMEVRERIHRAINDNTNSRVVLWSFFEALVLVAMTLGQIYYLKRFFEVRRVV 208          
BLAST of transmembrane emp24 domain-containing protein 2-like vs. Ensembl Cavefish
Match: TMED2 (transmembrane emp24 domain-containing protein 2 [Source:NCBI gene;Acc:103022411])

HSP 1 Score: 123.635 bits (309), Expect = 7.873e-35
Identity = 66/190 (34.74%), Postives = 104/190 (54.74%), Query Frame = 1
Query:   70 SSFEINLNPNSEECLFDYFNKSDKVEFSYEVINGGFLDIESKLLSPMKVEFMRRDKGSYGVYDLVIEQSGLYYFCLINKEGSQVEKTVRF--NLFSEPH---YRWERNITSRESKNSDLEEIVHDLSFLMHSVKAESEYSLTREQIHDQANRSANRHFLLWGLFEMVLMVAMSLGQIAYLKHLFEVRRFV 624
            S + ++++ ++EEC ++  N   K+   +EV  GGFLDI+ ++  P   +  + D+ S G Y +     G Y FC  NK  +   K V F  ++   P       E    + ++  + LEE++++L+  M +VK E EY   RE+IH   N + N   +LW  FE +++VAM+LGQI YLK  FEVRR V
Sbjct:   19 SGYFVSVDAHAEECFYERVNSGTKMGLMFEVAEGGFLDIDVEITGPDGKQIYKGDRESSGKYSVAAHMDGTYKFCFSNKMSTMTPKIVMFTIDIGEAPKGDGMETEVGGGTWDAHQNKLEEMINELAVAMTAVKHEQEYMEVRERIHRAINDNTNSRVVLWSFFEALVLVAMTLGQIYYLKRFFEVRRVV 208          
BLAST of transmembrane emp24 domain-containing protein 2-like vs. Ensembl Cavefish
Match: TMED2 (transmembrane emp24 domain-containing protein 2 [Source:NCBI gene;Acc:103022411])

HSP 1 Score: 122.479 bits (306), Expect = 2.303e-34
Identity = 66/195 (33.85%), Postives = 105/195 (53.85%), Query Frame = 1
Query:   70 SSFEINLNPNSEECLFDYFNKSDKVEFSYEVINGGFLDIESKLLSPMKVEFMRRDKGSYGVYDLVIEQSGLYYFCLINKEGSQVEKTVRFNL----------FSEPHYRWERNITSRESKNSDLEEIVHDLSFLMHSVKAESEYSLTREQIHDQANRSANRHFLLWGLFEMVLMVAMSLGQIAYLKHLFEVRRFV 624
            S + ++++ ++EEC ++  N   K+   +EV  GGFLDI+ ++  P   +  + D+ S G Y +     G Y FC  NK  +   K V F +                  + ++T+ ++K   LEE++++L+  M +VK E EY   RE+IH   N + N   +LW  FE +++VAM+LGQI YLK  FEVRR V
Sbjct:   19 SGYFVSVDAHAEECFYERVNSGTKMGLMFEVAEGGFLDIDVEITGPDGKQIYKGDRESSGKYSVAAHMDGTYKFCFSNKMSTMTPKIVMFTIDIGEAPKGDGMETEGMIGDCSVTAHQNK---LEEMINELAVAMTAVKHEQEYMEVRERIHRAINDNTNSRVVLWSFFEALVLVAMTLGQIYYLKRFFEVRRVV 210          
BLAST of transmembrane emp24 domain-containing protein 2-like vs. Ensembl Cavefish
Match: tmed5 (transmembrane emp24 domain-containing protein 5 [Source:NCBI gene;Acc:103032944])

HSP 1 Score: 61.6178 bits (148), Expect = 2.730e-11
Identity = 54/197 (27.41%), Postives = 90/197 (45.69%), Query Frame = 1
Query:   70 SSFEINLNPNSEECLFDYFNKSDKVEFSYEVINGGFLDIESKLLSPMKVEFMRRDKGSYGVYDLVIEQSGLYYFCLINKEGSQVEKTVRFNLF-------SEPHYRWERNITSRESKNSDLEEIVHDLSFLMHSVKAESEYSLTREQI------HDQANRSANRHFL-LWGLFEMVLMVAMSLGQIAYLKHLFEVRR 618
            S F   L P  +EC F    K   +E  Y+V++G  LD++  L SP     +   + S GV+ +  E+ G Y FC  N   +  EK + F L         +P   W+  +   +  +  LE+I+      ++SVKA    SL  + +       D+  + +N   + +W    +VLMV +S  Q+  L+ LF+ +R
Sbjct:   31 SDFTFTLPPGHKECFFQTMRKDASLEIEYQVLDGAGLDVDFYLSSPSGHVLVSDFRKSDGVHTVETEE-GDYMFCFDNTFSTVSEKIIFFELILDNMDDGQDPE-DWKEFVQGADLLDMKLEDIMD----TINSVKARLGKSLQIQTVLRAFEARDRNLQESNYERVNVWSCTNVVLMVVVSCIQVYLLRSLFDDKR 221          
BLAST of transmembrane emp24 domain-containing protein 2-like vs. Ensembl Sea Lamprey
Match: tmed2 (transmembrane p24 trafficking protein 2 [Source:ZFIN;Acc:ZDB-GENE-030131-269])

HSP 1 Score: 95.9005 bits (237), Expect = 1.263e-25
Identity = 54/144 (37.50%), Postives = 77/144 (53.47%), Query Frame = 1
Query:  193 KLLSPMKVEFMRRDKGSYGVYDLVIEQSGLYYFCLINKEGSQVEKTVRFNLFSEPHYRWERNITSRESKNSDLEEIVHDLSFLMHSVKAESEYSLTREQIHDQANRSANRHFLLWGLFEMVLMVAMSLGQIAYLKHLFEVRRFV 624
            ++  P   E  R D+ S G Y       G+Y FC  NK  +   K V F +      + +   T  E+  + LEE++++L+  M +VK E EY   RE+IH   N + N   +LW  FE +++VAM+LGQI YLK  FEVRR V
Sbjct:    2 QITGPDGREIYRGDRESNGKYTFAAHMDGVYKFCFGNKMSTMTPKMVMFTIDMGETPKGQDMET--EAHQNKLEEMINELATAMTAVKHEQEYMEVRERIHRSVNDNTNSRVVLWAFFEALVLVAMTLGQIYYLKRFFEVRRVV 143          
BLAST of transmembrane emp24 domain-containing protein 2-like vs. Ensembl Sea Lamprey
Match: tmed3 (transmembrane p24 trafficking protein 3 [Source:ZFIN;Acc:ZDB-GENE-030131-8335])

HSP 1 Score: 62.7734 bits (151), Expect = 2.247e-12
Identity = 42/178 (23.60%), Postives = 80/178 (44.94%), Query Frame = 1
Query:   88 LNPNSEECLFDYFNKSDKVEFSYEVINGGFLDIESKLLSPMKVEFMRRDKGSYGVYDLVIEQSGLYYFCLINKEGSQVEKTVRFNLFSEPHYRWERNITSRESKNSDLEEIVHDLSFLMHSVKA-ESEYSLTREQIHDQANRSANRHFLLWGLFEMVLMVAMSLGQIAYLKHLFEVRR 618
            L  N+++C ++  ++  KV   ++VI GG  D++ ++  P        ++  Y  Y    EQ G Y  C  N+  +   KTV F+L         R+  +R    + +E     +   + SV   ++++ L   Q   QA    N    +W + E +++V + +GQ+  LK  F  ++
Sbjct:   49 LKDNAKQCFYEDIDQGVKVTLDFQVITGGNYDVDCRVKGPHDSVLYNENRKQYDSYAWTTEQRGTYEICFSNEFSTFTHKTVYFDLQVGEEAPLFRDEGTRVVALTQMESACVTIHEGLKSVDNYQTQHRLQETQDRHQAE-VLNSRVNMWSIGETIILVVVGVGQVMLLKSFFSDKK 225          
BLAST of transmembrane emp24 domain-containing protein 2-like vs. Ensembl Sea Lamprey
Match: tmed5 (transmembrane p24 trafficking protein 5 [Source:ZFIN;Acc:ZDB-GENE-040426-1302])

HSP 1 Score: 53.1434 bits (126), Expect = 5.873e-9
Identity = 48/198 (24.24%), Postives = 83/198 (41.92%), Query Frame = 1
Query:   70 SSFEINLNPNSEECLFDYFNKSDKVEFSYEVINGGFLDIESKLLSPMKVEFMRRDKGSYGVYDLVIEQSGLYYFCLINKEGSQVEKTVRFNLF------SEPHYRWERNITSRESKNSDLEEIVHDLSFLMHSVKAESEYS------LTREQIHDQANRSANRHFL-LWGLFEMVLMVAMSLGQIAYLKHLFEVRRFV 624
            S    +L P   EC +        VE  Y+VI+GG LD++  + +P     +   +   GV+ + +++ G Y  C  N      EK + F +        E    W   I++       LE    DL   ++SVKA    S      L   +  D+  + +N   + LW    ++ ++ +S  Q+  ++ LFE +R V
Sbjct:   37 SDLTFSLAPGHRECFYQPVKPGTVVEIEYQVIDGGELDVDFTVTAPSGTVLLAEHRQGDGVHSVDVKEEGDYEICFDNSFSHISEKLIFFEILLDHMQPEEERPEWMDAISADHLIGMKLE----DLQESLNSVKARLSRSHQIQTVLRAFEARDRNLQESNLARVNLWSAASVLALMGVSALQVYLVRSLFEDKRRV 230          
BLAST of transmembrane emp24 domain-containing protein 2-like vs. Ensembl Sea Lamprey
Match: tmed5 (transmembrane p24 trafficking protein 5 [Source:ZFIN;Acc:ZDB-GENE-040426-1302])

HSP 1 Score: 49.6766 bits (117), Expect = 9.362e-8
Identity = 45/194 (23.20%), Postives = 82/194 (42.27%), Query Frame = 1
Query:   76 FEINLNPNSEECLFDYFNKSDKVEFSYEVINGGFLDIESKLLSPMKVEFMRRDKGSYGVYDLVIEQSGLYYFCLINKEGSQVEKTVRFNLFSEPHYRWERNITSRESKNSDLEEIV----HDLSFLMHSVKAESEYSLTREQI------HDQANRSANRHFL-LWGLFEMVLMVAMSLGQIAYLKHLFEVRRFV 624
            F  +L     EC +        +E  Y+VI+G  +D++  + SP         + S G+  +  +Q G Y FC  N      EK V F +  + +   E++         +++EI+     DL   M ++K     S+  + +       D+  +  N   + LW    MV++V ++  Q+  L+ LF  +R V
Sbjct:   30 FTFSLPAGRTECFYQALTAGASMEVEYQVIDGAGMDVDFSISSPTGQVLFAEYRKSEGLQSVEAKQDGDYMFCFDNSFSHVSEKVVFFEVILD-NMEVEKDDKPAWKDFVEVDEIIDMKLEDLQETMDNIKTRLGKSIQMQSVLRVYEARDRKLQETNFEMVNLWSGISMVVLVMVAGLQVYTLRSLFNDQRRV 222          
BLAST of transmembrane emp24 domain-containing protein 2-like vs. Ensembl Sea Lamprey
Match: tmed7 (transmembrane p24 trafficking protein 7 [Source:ZFIN;Acc:ZDB-GENE-040426-2570])

HSP 1 Score: 43.8986 bits (102), Expect = 8.322e-6
Identity = 39/182 (21.43%), Postives = 73/182 (40.11%), Query Frame = 1
Query:   82 INLNPNSEECLFDYFNKSDKVEFSYEVINGGFLDIESKLLSPMKVEFMRRDKGSYGVYDLVIEQSGLYYFCLINKEGSQVEKTVRFNLF---SEPHYRWERN----ITSRESKNSDLEEIVHDLSFLMHSVKAESEYSLTREQIHDQANRSANRHFLLWGLFEMVLMVAMSLGQIAYLKHLF 606
            + L      CLF            ++VI GG  D++ ++  P      +  +  Y  +    E+ G+Y  C  N+  +   KTV F+      +P  +   N    +T  ES    + E +  +      +  ++ + L   Q   QA    N    LW + E ++++++ +GQ+  LK  F
Sbjct:   32 LGLPAQQCRCLFMPIKVGRTCSKEWQVITGGNYDVDCRVEGPSGEILYKEMRKQYDSHVWTAEKKGIYAICFSNEFSTFSHKTVYFDWQIGDEKPVGKASTNGAVSLTQMESACVTIHEALKSV------ISYQTHHRLEEAQDRYQAEH-LNSRVTLWSVGETLMLLSVVVGQVMLLKSFF 206          
BLAST of transmembrane emp24 domain-containing protein 2-like vs. Ensembl Yeast
Match: EMP24 (Component of the p24 complex; role in misfolded protein quality control; binds to GPI anchor proteins and mediates their efficient transport from the ER to the Golgi; integral membrane protein that associates with endoplasmic reticulum-derived COPII-coated vesicles [Source:SGD;Acc:S000003168])

HSP 1 Score: 62.7734 bits (151), Expect = 9.632e-13
Identity = 55/219 (25.11%), Postives = 92/219 (42.01%), Query Frame = 1
Query:    4 IASIMNKLHFALFVHIIIINNLSSFEINLNPNSEECLFDYFNKSDKVEFSYEV----------INGGFLDIESKLLSPMKVEFMR--RDKGSYGVYDLVIEQSGLYYFCLINKEGSQVEKTVRFNLFSEPHYRWERNITSRESKNSDLEEIVHDLSFLMHSVKAESEYSLTREQIHDQANRSANRHFLLWGLFEMVLMVAMSLGQIAYLKHLFEVRRFV 624
            +AS   K   A F+      + S+  + L      C F+  +K D++  S++           + G F+     +  P + E ++  RD  S+G   L     G + +C +N+      K V FN+    +   +      +   + L+  V  LS L   VK E  Y + RE+ H     S N     W +F++ +++A SL QI YL+  FEV   V
Sbjct:    1 MASFATKFVIACFLFF----SASAHNVLLPAYGRRCFFEDLSKGDELSISFQFGDRNPQSSSQLTGDFI-----IYGPERHEVLKTVRDT-SHGEITLSAPYKGHFQYCFLNENTGIETKDVTFNIHGVVYVDLD------DPNTNTLDSAVRKLSKLTREVKDEQSYIVIRERTHRNTAESTNDRVKWWSIFQLGVVIANSLFQIYYLRRFFEVTSLV 203          
BLAST of transmembrane emp24 domain-containing protein 2-like vs. Ensembl Yeast
Match: ERP4 (Member of the p24 family involved in ER to Golgi transport; similar to Emp24p and Erv25p; ERP4 has a paralog, ERP2, that arose from the whole genome duplication [Source:SGD;Acc:S000005542])

HSP 1 Score: 43.1282 bits (100), Expect = 7.574e-6
Identity = 50/193 (25.91%), Postives = 86/193 (44.56%), Query Frame = 1
Query:   61 NNLSSFEINLNPNSEECLF-DYFNKSDKVEFSYEVINGGFLDIESKLLSPMKVEFMRRDKGSYGVYDLVIEQSGLYYFCLINKEGSQVEKTVRFNLFSEPHYRWERNITSRESK-----NSDLEEIVHDLSFLMHSVKAESEYSLTREQIHDQANRSANRHFLLW-GLFEMVLMVAMSLGQIAYLKHLFEVRR 618
            +N +   I+L   ++ECL+ D  +  D +  SY+V+ GG  +I+  + +P     +   +  +  + L     G Y FCL N  G+  +K V   L  E        ++S ESK     N+ +EEI  +L    + +    +Y   RE   +    S+    L W  L  M +MV +S+ Q   ++  F  R+
Sbjct:   22 SNYAPVGISLPAFTKECLYYDLSSDKDVLVVSYQVLTGGNFEIDFDITAPDGSVIVTERQKKHSDFLLKSFGIGKYTFCLSNNYGTSPKK-VEITLEKEKEI-----VSSHESKEDIIANNAIEEIDRNL----NKITKTMDYLRAREW-RNMYTVSSTESRLTWLSLLIMGVMVGISIVQALIIQFFFTSRQ 203          
BLAST of transmembrane emp24 domain-containing protein 2-like vs. Ensembl Nematostella
Match: EDO31547 (Transmembrane emp24 domain-containing protein [Source:UniProtKB/Swiss-Prot;Acc:A7SXK3])

HSP 1 Score: 110.538 bits (275), Expect = 2.632e-30
Identity = 62/183 (33.88%), Postives = 94/183 (51.37%), Query Frame = 1
Query:   82 INLNPNSEECLFDYFNKSDKVEFSYEVINGGFLDIESKLLSPMKVEFMRRDKGSYGVYDLVIEQSGLYYFCLINKEGSQVEKTVRFNL-FSEPHYRWERNITSRESKNSD-LEEIVHDLSFLMHSVKAESEYSLTREQIHDQANRSANRHFLLWGLFEMVLMVAMSLGQIAYLKHLFEVRRFV 624
            I ++ + EEC  D      K+   +EV  GGFLDI+ K++ P +      ++ + G Y       G Y +C  NK  +   K ++F++   E      ++ +  ++   D L E+V  LS  M  VK E EY   RE+IH   N + N   + W  FE +++VAM+LGQ+ YLK  FEVRR V
Sbjct:   25 ITIDAHGEECFHDKVTSGTKMGLIFEVAEGGFLDIDVKIIGPDQKVIYSGERETSGKYTFAAHMDGTYNYCFSNKMSTMTPKVLKFSMDIGEA----PKDTSKEDNAGHDKLSEMVSQLSEAMTGVKHEQEYMEVRERIHRSINDNTNSRVVWWSFFESLVLVAMTLGQVYYLKRFFEVRRVV 203          
BLAST of transmembrane emp24 domain-containing protein 2-like vs. Ensembl Nematostella
Match: EDO42051 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S2I7])

HSP 1 Score: 63.929 bits (154), Expect = 9.765e-13
Identity = 46/205 (22.44%), Postives = 87/205 (42.44%), Query Frame = 1
Query:    7 ASIMNKLHFALFVHIIIINNLSSFEINLNPNSEECLFDYFNKSDKVEFSYEVINGGFLDIESKLLSPMKVEFMRRDKGSYGVYDLVIEQSGLYYFCLINKEGSQVEKTVRFNLFSEPHYRWERNITSRESKNSDLEEIVHDL-SFLMHSVKAESEYSLTREQIHDQANRSANRHFLLWGLFEMVLMVAMSLGQIAYLKHLFEVRR 618
            + I + + F +     I+ + +     L  N+ +C ++   K  K    ++VI GG  D++  L    + E     K  Y  +   +EQ G+Y FC  N+  +   KTV F+  S    +  + + +  +  + +E     +   L  ++  ++ + L   Q  D A    N     W + E VL+V + + Q+  L+  F  RR
Sbjct:    3 SCIQSFVTFTVLALSCILVSCTELTFELEDNARQCFYEELKKDAKATLEFQVITGGNYDVDVILYDINEKELYSVQKKQYDSHTFTVEQPGIYKFCFSNEFSTISHKTVYFDFQSGEETQLTKTMGNHHTALTQMETACVTIHENLKIAIDYQTHHRLRESQGRDMAEY-LNERVQWWSVGESVLIVIVGICQVFILRQFFAERR 206          
BLAST of transmembrane emp24 domain-containing protein 2-like vs. Ensembl Nematostella
Match: EDO35763 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SKI6])

HSP 1 Score: 58.9214 bits (141), Expect = 8.550e-11
Identity = 46/191 (24.08%), Postives = 87/191 (45.55%), Query Frame = 1
Query:   70 SSFEINLNPNSEECLFDYFNKSDKVEFSYEVINGGFLDIESKLLSPMKVEFMRRDKGSYGVYDLVIEQSGLYYFCLINKEGSQVEKTVRFNLFSEPHYRWERNITSRES-KNSDLEEIVHD-LSFLMHSVKAESEYSLTREQIHDQANRSANRHFLL----------WGLFEMVLMVAMSLGQIAYLKHLF 606
            S F   ++P   +C ++   ++  ++  Y+VI GG LDI   L +P     +     +   + LV E+ G Y FC  N   +   K +    F++    +E +I      K+ +LEE +HD     + +V+ + + +   E I +  + +A R F L          W L + V ++ +++ Q+  +KH F
Sbjct:   33 SGFTFRVDPGKRDCFYETIKRNVTLDVEYQVIEGGDLDIGFTLQAPSGKIAIEDGHRTDEAHSLVTEEEGEYEFCFDNTFSTVASKAI----FADLGVDFEDDIQKMIGIKDENLEEDLHDRFVRTLENVRVDLDKA---EHIQNFISSAAARDFRLAKSKDSRVFWWSLIQSVALIGVAIVQVNVVKHFF 216          
BLAST of transmembrane emp24 domain-containing protein 2-like vs. Ensembl Medaka
Match: tmed2 (transmembrane emp24 domain-containing protein 2 [Source:NCBI gene;Acc:101161906])

HSP 1 Score: 128.257 bits (321), Expect = 1.030e-36
Identity = 66/191 (34.55%), Postives = 106/191 (55.50%), Query Frame = 1
Query:   52 IIINNLSSFEINLNPNSEECLFDYFNKSDKVEFSYEVINGGFLDIESKLLSPMKVEFMRRDKGSYGVYDLVIEQSGLYYFCLINKEGSQVEKTVRFNLFSEPHYRWERNITSRESKNSDLEEIVHDLSFLMHSVKAESEYSLTREQIHDQANRSANRHFLLWGLFEMVLMVAMSLGQIAYLKHLFEVRRFV 624
            ++    S + ++++ ++EEC ++  N   K+   +EV  GGFLDI+ +++ P   +  + D+ S G Y +     G Y FC  NK  +   K V F +      + +   T  E+  + LEE++++L+  M +VK E EY   RE+IH   N + N   +LW  FE +++VAM+LGQI YLK  FEVRR V
Sbjct:   13 VLTATASGYFVSIDAHAEECFYERVNSGTKMGLMFEVAEGGFLDIDVEIMGPDGKQIYKGDRESSGKYSVAAHMDGTYKFCFSNKMSTMTPKIVMFTIDIGEAPKGQNMET--EAHQNKLEEMINELAVAMTAVKHEQEYMEVRERIHRAINDNTNSRVVLWSFFEALVLVAMTLGQIYYLKRFFEVRRVV 201          
BLAST of transmembrane emp24 domain-containing protein 2-like vs. Ensembl Medaka
Match: tmed2 (transmembrane emp24 domain-containing protein 2 [Source:NCBI gene;Acc:101161906])

HSP 1 Score: 126.331 bits (316), Expect = 5.127e-36
Identity = 67/202 (33.17%), Postives = 107/202 (52.97%), Query Frame = 1
Query:   52 IIINNLSSFEINLNPNSEECLFDYFNKSDKVEFSYEVINGGFLDIESKLLSPMKVEFMRRDKGSYGVYDLVIEQSGLYYFCLINKEGSQVEKTVRF-----------NLFSEPHYRWERNITSRESKNSDLEEIVHDLSFLMHSVKAESEYSLTREQIHDQANRSANRHFLLWGLFEMVLMVAMSLGQIAYLKHLFEVRRFV 624
            ++    S + ++++ ++EEC ++  N   K+   +EV  GGFLDI+ +++ P   +  + D+ S G Y +     G Y FC  NK  +   K V F           N+ +E    W+       +  + LEE++++L+  M +VK E EY   RE+IH   N + N   +LW  FE +++VAM+LGQI YLK  FEVRR V
Sbjct:   13 VLTATASGYFVSIDAHAEECFYERVNSGTKMGLMFEVAEGGFLDIDVEIMGPDGKQIYKGDRESSGKYSVAAHMDGTYKFCFSNKMSTMTPKIVMFTIDIGEAPKGQNMETEGANSWD-------AHQNKLEEMINELAVAMTAVKHEQEYMEVRERIHRAINDNTNSRVVLWSFFEALVLVAMTLGQIYYLKRFFEVRRVV 207          
BLAST of transmembrane emp24 domain-containing protein 2-like vs. Ensembl Medaka
Match: tmed2 (transmembrane emp24 domain-containing protein 2 [Source:NCBI gene;Acc:101161906])

HSP 1 Score: 84.3445 bits (207), Expect = 4.743e-20
Identity = 44/148 (29.73%), Postives = 78/148 (52.70%), Query Frame = 1
Query:   52 IIINNLSSFEINLNPNSEECLFDYFNKSDKVEFSYEVINGGFLDIESKLLSPMKVEFMRRDKGSYGVYDLVIEQSGLYYFCLINKEGSQVEKTVRFNLFSEPHYRWERNITSRESKNSDLEEIVHDLSFLMHSVKAESEYSLTREQIH 495
            ++    S + ++++ ++EEC ++  N   K+   +EV  GGFLDI+ +++ P   +  + D+ S G Y +     G Y FC  NK  +   K V F +      + +   T  E+  + LEE++++L+  M +VK E EY   RE+IH
Sbjct:   13 VLTATASGYFVSIDAHAEECFYERVNSGTKMGLMFEVAEGGFLDIDVEIMGPDGKQIYKGDRESSGKYSVAAHMDGTYKFCFSNKMSTMTPKIVMFTIDIGEAPKGQNMET--EAHQNKLEEMINELAVAMTAVKHEQEYMEVRERIH 158          
BLAST of transmembrane emp24 domain-containing protein 2-like vs. Ensembl Medaka
Match: tmed2 (transmembrane emp24 domain-containing protein 2 [Source:NCBI gene;Acc:101161906])

HSP 1 Score: 84.3445 bits (207), Expect = 4.743e-20
Identity = 44/148 (29.73%), Postives = 78/148 (52.70%), Query Frame = 1
Query:   52 IIINNLSSFEINLNPNSEECLFDYFNKSDKVEFSYEVINGGFLDIESKLLSPMKVEFMRRDKGSYGVYDLVIEQSGLYYFCLINKEGSQVEKTVRFNLFSEPHYRWERNITSRESKNSDLEEIVHDLSFLMHSVKAESEYSLTREQIH 495
            ++    S + ++++ ++EEC ++  N   K+   +EV  GGFLDI+ +++ P   +  + D+ S G Y +     G Y FC  NK  +   K V F +      + +   T  E+  + LEE++++L+  M +VK E EY   RE+IH
Sbjct:   13 VLTATASGYFVSIDAHAEECFYERVNSGTKMGLMFEVAEGGFLDIDVEIMGPDGKQIYKGDRESSGKYSVAAHMDGTYKFCFSNKMSTMTPKIVMFTIDIGEAPKGQNMET--EAHQNKLEEMINELAVAMTAVKHEQEYMEVRERIH 158          
BLAST of transmembrane emp24 domain-containing protein 2-like vs. Ensembl Medaka
Match: tmed2 (transmembrane emp24 domain-containing protein 2 [Source:NCBI gene;Acc:101161906])

HSP 1 Score: 84.3445 bits (207), Expect = 4.743e-20
Identity = 44/148 (29.73%), Postives = 78/148 (52.70%), Query Frame = 1
Query:   52 IIINNLSSFEINLNPNSEECLFDYFNKSDKVEFSYEVINGGFLDIESKLLSPMKVEFMRRDKGSYGVYDLVIEQSGLYYFCLINKEGSQVEKTVRFNLFSEPHYRWERNITSRESKNSDLEEIVHDLSFLMHSVKAESEYSLTREQIH 495
            ++    S + ++++ ++EEC ++  N   K+   +EV  GGFLDI+ +++ P   +  + D+ S G Y +     G Y FC  NK  +   K V F +      + +   T  E+  + LEE++++L+  M +VK E EY   RE+IH
Sbjct:   13 VLTATASGYFVSIDAHAEECFYERVNSGTKMGLMFEVAEGGFLDIDVEIMGPDGKQIYKGDRESSGKYSVAAHMDGTYKFCFSNKMSTMTPKIVMFTIDIGEAPKGQNMET--EAHQNKLEEMINELAVAMTAVKHEQEYMEVRERIH 158          
BLAST of transmembrane emp24 domain-containing protein 2-like vs. Planmine SMEST
Match: SMESG000078201.1 (SMESG000078201.1)

HSP 1 Score: 415.231 bits (1066), Expect = 6.487e-150
Identity = 202/203 (99.51%), Postives = 203/203 (100.00%), Query Frame = 1
Query:   16 MNKLHFALFVHIIIINNLSSFEINLNPNSEECLFDYFNKSDKVEFSYEVINGGFLDIESKLLSPMKVEFMRRDKGSYGVYDLVIEQSGLYYFCLINKEGSQVEKTVRFNLFSEPHYRWERNITSRESKNSDLEEIVHDLSFLMHSVKAESEYSLTREQIHDQANRSANRHFLLWGLFEMVLMVAMSLGQIAYLKHLFEVRRFV 624
            MNKLHFALFVHIIIINNLSSFEINLNPNSEECLFDYFNKSDKVEFSYEVINGGFLDIESKLLSPMKVEFMRRDKGSYGVYDLVIEQSGLYYFCLINKEGSQVEKTVRFNLFSEPHYRWERNITSRESKNSDLEEIVHDLSFLMHSVKAESEYSLTREQIHDQANRSANRHFLLWGLFEMVLMVAMS+GQIAYLKHLFEVRRFV
Sbjct:    1 MNKLHFALFVHIIIINNLSSFEINLNPNSEECLFDYFNKSDKVEFSYEVINGGFLDIESKLLSPMKVEFMRRDKGSYGVYDLVIEQSGLYYFCLINKEGSQVEKTVRFNLFSEPHYRWERNITSRESKNSDLEEIVHDLSFLMHSVKAESEYSLTREQIHDQANRSANRHFLLWGLFEMVLMVAMSIGQIAYLKHLFEVRRFV 203          
BLAST of transmembrane emp24 domain-containing protein 2-like vs. Planmine SMEST
Match: SMESG000069470.1 (SMESG000069470.1)

HSP 1 Score: 122.094 bits (305), Expect = 1.780e-34
Identity = 67/200 (33.50%), Postives = 108/200 (54.00%), Query Frame = 1
Query:   31 FALFVHIIIINNLSSFEINLNPNSEECLFDYFNKSDKVEFSYEVINGGFLDIESKLLSPMKVEFMRRDKGSYGVYDLVIEQSGLYYFCLINKEGSQVEKTVRFNL--FSEPHYRWERNITSRESKNSDLEEIVHDLSFLMHSVKAESEYSLTREQIHDQANRSANRHFLLWGLFEMVLMVAMSLGQIAYLKHLFEVRRFV 624
            F +  H+I    +  + I ++ NS+EC FD      K+   +EV+ GGFLDI+ K+  P        D+ S G Y       G+Y +C  NK  +   K + F++    +P    E N  + ++K   LE++V++LS  + +VK + EY   RE++H   N + N   +LW  FE VL+++M++GQ+ YL+  FEVRR V
Sbjct:    7 FLILCHLIF--KIKGYYITVDANSDECFFDRLVSGAKMMLMFEVVEGGFLDIDVKVFGPDGKAIYTGDRESNGKYAFSAHVDGIYKYCFSNKMSTMTPKIIMFSMDVSQKPRENKEMNTDAHQNK---LEDMVYELSNSLLNVKHQQEYLEVRERVHRSINENTNSRVVLWSAFEAVLLLSMTIGQVYYLRRFFEVRRVV 201          
BLAST of transmembrane emp24 domain-containing protein 2-like vs. Planmine SMEST
Match: SMESG000026239.1 (SMESG000026239.1)

HSP 1 Score: 117.857 bits (294), Expect = 5.788e-33
Identity = 72/203 (35.47%), Postives = 106/203 (52.22%), Query Frame = 1
Query:   16 MNKLHFALFVHIIIINNLSSFEINLNPNSEECLFDYFNKSDKVEFSYEVINGGFLDIESKLLSPMKVEFMRRDKGSYGVYDLVIEQSGLYYFCLINKEGSQVEKTVRFNLFSEPHYRWERNITSRESKNSDLEEIVHDLSFLMHSVKAESEYSLTREQIHDQANRSANRHFLLWGLFEMVLMVAMSLGQIAYLKHLFEVRRFV 624
            M K+   LF   I++++  SF I+++PN EEC FD       +   YEV  GG LDI++K+L P         K S   +    +  G+Y +C  N   S   K + F+L +    + +         +S LEE+V +L   + +VK E EY  TRE+IH + N + N   LLW +FE  +++ MS GQ+ YLK  FEVRR V
Sbjct:    1 MYKIFCVLF---ILLHSALSFFISVSPNVEECFFDRIKPDTDMLLLYEVAEGGSLDIDAKVLDPSGSILYSAVKKSDDKFTFRTKVDGIYKYCFSNTMSSVTNKILMFSLDTISPAKKDS-----PDSHSKLEEMVSELDKALTTVKHEQEYMETREKIHRKINENTNSRVLLWAIFEACVLIIMSAGQVYYLKRFFEVRRVV 195          
BLAST of transmembrane emp24 domain-containing protein 2-like vs. Planmine SMEST
Match: SMESG000025949.1 (SMESG000025949.1)

HSP 1 Score: 106.301 bits (264), Expect = 1.976e-28
Identity = 57/180 (31.67%), Postives = 93/180 (51.67%), Query Frame = 1
Query:   82 INLNPNSEECLFDYFNKSDKVEFSYEVINGGFLDIESKLLSPMKVEFMRRDKGSYGVYDLVIEQSGLYYFCLINKEGSQVEKTVRFNL-FSEPHYRWERNITSRESKNSDLEEIVHDLSFLMHSVKAESEYSLTREQIHDQANRSANRHFLLWGLFEMVLMVAMSLGQIAYLKHLFEVRR 618
            I + P   EC FD  N +D++   +EVI GG++DI  +L  P K    + D+ S G Y     Q G Y +C  N    +  K V FN+    P       +  +E ++S +  ++ ++S  +  VK E ++   R Q+H + N   N++ LLW + E+  ++ +SLGQ+ YL+  FE RR
Sbjct:   23 IIIEPKETECFFDKANTNDRIVLMFEVIEGGYMDINIQLFGPDKKVIYQGDRESNGKYTFATNQPGKYRYCFSNSMNDKDRKIVLFNMDIITP------KVDQKEPQDS-MINLISEMSVRIFDVKHEVQFLKARHQVHYEINEKTNKYILLWVIIEISCLICLSLGQVLYLRRYFEARR 195          
BLAST of transmembrane emp24 domain-containing protein 2-like vs. Planmine SMEST
Match: SMESG000069470.1 (SMESG000069470.1)

HSP 1 Score: 103.605 bits (257), Expect = 8.813e-28
Identity = 56/159 (35.22%), Postives = 89/159 (55.97%), Query Frame = 1
Query:  154 YEVINGGFLDIESKLLSPMKVEFMRRDKGSYGVYDLVIEQSGLYYFCLINKEGSQVEKTVRFNL--FSEPHYRWERNITSRESKNSDLEEIVHDLSFLMHSVKAESEYSLTREQIHDQANRSANRHFLLWGLFEMVLMVAMSLGQIAYLKHLFEVRRFV 624
            +EV+ GGFLDI+ K+  P        D+ S G Y       G+Y +C  NK  +   K + F++    +P    E N  + ++K   LE++V++LS  + +VK + EY   RE++H   N + N   +LW  FE VL+++M++GQ+ YL+  FEVRR V
Sbjct:    2 FEVVEGGFLDIDVKVFGPDGKAIYTGDRESNGKYAFSAHVDGIYKYCFSNKMSTMTPKIIMFSMDVSQKPRENKEMNTDAHQNK---LEDMVYELSNSLLNVKHQQEYLEVRERVHRSINENTNSRVVLWSAFEAVLLLSMTIGQVYYLRRFFEVRRVV 157          
The following BLAST results are available for this feature:
BLAST of transmembrane emp24 domain-containing protein 2-like vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
TMED27.613e-3435.14transmembrane p24 trafficking protein 2 [Source:HG... [more]
TMED22.578e-2039.66transmembrane p24 trafficking protein 2 [Source:HG... [more]
TMED21.016e-1629.25transmembrane p24 trafficking protein 2 [Source:HG... [more]
TMED79.025e-1022.22transmembrane p24 trafficking protein 7 [Source:HG... [more]
TMED37.813e-822.78transmembrane p24 trafficking protein 3 [Source:HG... [more]
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BLAST of transmembrane emp24 domain-containing protein 2-like vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 2
Match NameE-valueIdentityDescription
sel-98.542e-3634.63Suppressor/enhancer of lin-12 protein 9 [Source:U... [more]
F57B10.51.151e-620.40pep chromosome:WBcel235:I:6562699:6563660:1 gene:W... [more]
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BLAST of transmembrane emp24 domain-containing protein 2-like vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CHOp246.706e-3233.51gene:FBgn0029709 transcript:FBtr0343566[more]
CHOp246.706e-3233.51gene:FBgn0029709 transcript:FBtr0070675[more]
CG93088.516e-2024.00gene:FBgn0034681 transcript:FBtr0071749[more]
p24-11.459e-922.39gene:FBgn0030341 transcript:FBtr0073604[more]
p24-11.459e-922.39gene:FBgn0030341 transcript:FBtr0334455[more]
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BLAST of transmembrane emp24 domain-containing protein 2-like vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
tmed21.076e-3535.68transmembrane p24 trafficking protein 2 [Source:ZF... [more]
tmed22.586e-3535.26transmembrane p24 trafficking protein 2 [Source:ZF... [more]
tmed71.616e-1021.29transmembrane p24 trafficking protein 7 [Source:ZF... [more]
tmed71.616e-1021.29transmembrane p24 trafficking protein 7 [Source:ZF... [more]
tmed71.616e-1021.29transmembrane p24 trafficking protein 7 [Source:ZF... [more]
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BLAST of transmembrane emp24 domain-containing protein 2-like vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
tmed26.611e-3435.14transmembrane p24 trafficking protein 2 [Source:NC... [more]
tmed25.610e-3334.21transmembrane p24 trafficking protein 2 [Source:NC... [more]
serinc21.669e-1123.30serine incorporator 2 [Source:Xenbase;Acc:XB-GENE-... [more]
serinc22.008e-1123.30serine incorporator 2 [Source:Xenbase;Acc:XB-GENE-... [more]
tmed54.704e-1026.26transmembrane p24 trafficking protein 5 [Source:NC... [more]
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BLAST of transmembrane emp24 domain-containing protein 2-like vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Tmed24.953e-3435.14transmembrane p24 trafficking protein 2 [Source:MG... [more]
Tmed22.766e-2235.42transmembrane p24 trafficking protein 2 [Source:MG... [more]
Tmed21.867e-1746.25transmembrane p24 trafficking protein 2 [Source:MG... [more]
Tmed76.837e-1022.58transmembrane p24 trafficking protein 7 [Source:MG... [more]
Tmed34.449e-823.08transmembrane p24 trafficking protein 3 [Source:MG... [more]
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BLAST of transmembrane emp24 domain-containing protein 2-like vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|O17528|TMED2_CAEEL1.087e-3434.63Suppressor/enhancer of lin-12 protein 9 OS=Caenorh... [more]
sp|P49020|TMED2_CRIGR3.130e-3335.14Transmembrane emp24 domain-containing protein 2 (F... [more]
sp|Q9R0Q3|TMED2_MOUSE3.465e-3335.14Transmembrane emp24 domain-containing protein 2 OS... [more]
sp|Q63524|TMED2_RAT3.656e-3335.14Transmembrane emp24 domain-containing protein 2 OS... [more]
sp|Q15363|TMED2_HUMAN3.656e-3335.14Transmembrane emp24 domain-containing protein 2 OS... [more]
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BLAST of transmembrane emp24 domain-containing protein 2-like vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1W4XF972.336e-3735.50transmembrane emp24 domain-containing protein 2-li... [more]
A8XDC71.443e-3634.63Protein CBR-SEL-9 OS=Caenorhabditis briggsae OX=62... [more]
A0A2H2IU331.491e-3635.50GOLD domain-containing protein OS=Caenorhabditis j... [more]
G0NHK81.544e-3535.12CBN-SEL-9 protein OS=Caenorhabditis brenneri OX=13... [more]
A0A2G5T5U91.690e-3534.02GOLD domain-containing protein OS=Caenorhabditis n... [more]
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BLAST of transmembrane emp24 domain-containing protein 2-like vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
tmed24.225e-3535.91transmembrane emp24 domain-containing protein 2 [S... [more]
TMED27.873e-3534.74transmembrane emp24 domain-containing protein 2 [S... [more]
TMED27.873e-3534.74transmembrane emp24 domain-containing protein 2 [S... [more]
TMED22.303e-3433.85transmembrane emp24 domain-containing protein 2 [S... [more]
tmed52.730e-1127.41transmembrane emp24 domain-containing protein 5 [S... [more]
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BLAST of transmembrane emp24 domain-containing protein 2-like vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
tmed21.263e-2537.50transmembrane p24 trafficking protein 2 [Source:ZF... [more]
tmed32.247e-1223.60transmembrane p24 trafficking protein 3 [Source:ZF... [more]
tmed55.873e-924.24transmembrane p24 trafficking protein 5 [Source:ZF... [more]
tmed59.362e-823.20transmembrane p24 trafficking protein 5 [Source:ZF... [more]
tmed78.322e-621.43transmembrane p24 trafficking protein 7 [Source:ZF... [more]
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BLAST of transmembrane emp24 domain-containing protein 2-like vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 2
Match NameE-valueIdentityDescription
EMP249.632e-1325.11Component of the p24 complex; role in misfolded pr... [more]
ERP47.574e-625.91Member of the p24 family involved in ER to Golgi t... [more]
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BLAST of transmembrane emp24 domain-containing protein 2-like vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 3
Match NameE-valueIdentityDescription
EDO315472.632e-3033.88Transmembrane emp24 domain-containing protein [So... [more]
EDO420519.765e-1322.44Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO357638.550e-1124.08Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of transmembrane emp24 domain-containing protein 2-like vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
tmed21.030e-3634.55transmembrane emp24 domain-containing protein 2 [S... [more]
tmed25.127e-3633.17transmembrane emp24 domain-containing protein 2 [S... [more]
tmed24.743e-2029.73transmembrane emp24 domain-containing protein 2 [S... [more]
tmed24.743e-2029.73transmembrane emp24 domain-containing protein 2 [S... [more]
tmed24.743e-2029.73transmembrane emp24 domain-containing protein 2 [S... [more]
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BLAST of transmembrane emp24 domain-containing protein 2-like vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000078201.16.487e-15099.51SMESG000078201.1[more]
SMESG000069470.11.780e-3433.50SMESG000069470.1[more]
SMESG000026239.15.788e-3335.47SMESG000026239.1[more]
SMESG000025949.11.976e-2831.67SMESG000025949.1[more]
SMESG000069470.18.813e-2835.22SMESG000069470.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30029791 ID=SMED30029791|Name=transmembrane emp24 domain-containing protein 2-like|organism=Schmidtea mediterranea sexual|type=transcript|length=653bp
TAAATTGCTTCGATAATGAACAAACTTCATTTTGCACTTTTTGTACATAT
TATAATAATAAATAATCTGAGTTCCTTTGAAATTAATCTGAATCCAAATA
GTGAGGAATGCTTATTTGACTATTTTAATAAATCAGACAAAGTTGAATTT
TCGTATGAAGTTATCAATGGCGGTTTTCTTGATATTGAATCAAAATTATT
GTCACCGATGAAAGTGGAATTTATGCGGCGGGATAAAGGTTCTTACGGCG
TCTATGACTTGGTGATTGAACAATCAGGGTTGTATTATTTTTGCCTAATT
AACAAAGAAGGATCTCAAGTAGAGAAAACAGTTAGATTTAATTTATTTTC
TGAACCCCATTATCGTTGGGAACGAAATATAACGTCTAGAGAAAGTAAAA
ATTCGGATCTTGAAGAAATAGTACATGATTTGAGCTTTCTCATGCATTCT
GTCAAAGCTGAATCAGAATATTCGTTAACTAGAGAACAGATTCATGACCA
AGCAAATAGATCTGCAAATCGTCATTTTCTACTTTGGGGTTTGTTCGAAA
TGGTTTTGATGGTCGCTATGAGTCTCGGACAAATTGCATATTTGAAGCAT
TTATTTGAAGTACGGAGATTTGTATGATGTATATAAAATATGATTTGAAT
GGA
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protein sequence of SMED30029791-orf-1

>SMED30029791-orf-1 ID=SMED30029791-orf-1|Name=SMED30029791-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=204bp
MNKLHFALFVHIIIINNLSSFEINLNPNSEECLFDYFNKSDKVEFSYEVI
NGGFLDIESKLLSPMKVEFMRRDKGSYGVYDLVIEQSGLYYFCLINKEGS
QVEKTVRFNLFSEPHYRWERNITSRESKNSDLEEIVHDLSFLMHSVKAES
EYSLTREQIHDQANRSANRHFLLWGLFEMVLMVAMSLGQIAYLKHLFEVR
RFV*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000074oocyte
PLANA:0000231vitelline gland
PLANA:0000418head
Vocabulary: INTERPRO
TermDefinition
IPR015720TMP21-related
IPR036598GOLD_dom_sf
IPR009038GOLD_dom
Vocabulary: biological process
TermDefinition
GO:0006810transport
GO:0001701in utero embryonic development
GO:0001756somitogenesis
GO:0001843neural tube closure
GO:0001892embryonic placenta development
GO:0001893maternal placenta development
GO:0001947heart looping
GO:0006886intracellular protein transport
GO:0006888ER to Golgi vesicle-mediated transport
GO:0006890retrograde vesicle-mediated transport, Golgi to ER
GO:0007030Golgi organization
GO:0010628positive regulation of gene expression
GO:0015031protein transport
GO:0016192vesicle-mediated transport
GO:0032525somite rostral/caudal axis specification
GO:0034260negative regulation of GTPase activity
GO:0035264multicellular organism growth
GO:0035459cargo loading into vesicle
GO:0036342post-anal tail morphogenesis
GO:0048205COPI coating of Golgi vesicle
GO:0048208COPII vesicle coating
GO:0048598embryonic morphogenesis
GO:0060716labyrinthine layer blood vessel development
GO:0072661protein targeting to plasma membrane
Vocabulary: cellular component
TermDefinition
GO:0016021integral component of membrane
GO:0000139Golgi membrane
GO:0005783endoplasmic reticulum
GO:0005789endoplasmic reticulum membrane
GO:0005793endoplasmic reticulum-Golgi intermediate compartment
GO:0005794Golgi apparatus
GO:0012507ER to Golgi transport vesicle membrane
GO:0016020membrane
GO:0030133transport vesicle
GO:0030137COPI-coated vesicle
GO:0030659cytoplasmic vesicle membrane
GO:0030663COPI-coated vesicle membrane
GO:0031410cytoplasmic vesicle
GO:0032580Golgi cisterna membrane
GO:0033116endoplasmic reticulum-Golgi intermediate compartment membrane
GO:0042589zymogen granule membrane
GO:0043231intracellular membrane-bounded organelle
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR009038GOLD domainSMARTSM01190EMP24_GP25L_2coord: 20..198
e-value: 4.2E-32
score: 122.6
IPR009038GOLD domainPFAMPF01105EMP24_GP25Lcoord: 21..198
e-value: 8.6E-28
score: 97.5
IPR009038GOLD domainPROSITEPS50866GOLDcoord: 30..112
score: 12.345
NoneNo IPR availablePANTHERPTHR22811:SF31TRANSMEMBRANE EMP24 DOMAIN-CONTAINING PROTEIN 2coord: 7..198
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..20
score: 0.619
NoneNo IPR availableTMHMMTMhelixcoord: 171..193
IPR015720TMP21-relatedPANTHERPTHR22811TRANSMEMBRANE EMP24 DOMAIN-CONTAINING PROTEINcoord: 7..198
IPR036598GOLD domain superfamilySUPERFAMILYSSF101576Supernatant protein factor (SPF), C-terminal domaincoord: 29..120