SMED30029781

Overview
NameSMED30029781
Smed IDSMED30029781
Length (bp)323
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30029781 (SMED30029781) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30029781 (SMED30029781) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30029781 (SMED30029781) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30029781 (SMED30029781) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30029781

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 8

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
parapharyngeal regionSMED30029781 SmedASXL_018789SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
pharynxSMED30029781 dd_Smed_v4_1213_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
protonephridiaSMED30029781 dd_Smed_v4_1213_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30029781 dd_Smed_v4_1213_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30029781 dd_Smed_v4_1213_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynxSMED30029781 dd_Smed_v6_1213_0dd_Smed_v6PMID:29674432
Plass et al., 2018		whole organism asexual adult colorimetric in situ hybridization evidence
pharynxSMED30029781 dd_Smed_v6_1213_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynx progenitor cellSMED30029781 dd_Smed_v6_1213_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30029781 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.004587:63596..63917 +1598
v31.024060:7170..7491 +1589
Homology
[Close]
BLAST of SMED30029781 vs. Ensembl Human
Match: CALML5 (calmodulin like 5 [Source:HGNC Symbol;Acc:HGNC:18180])

HSP 1 Score: 50.447 bits (119), Expect = 1.867e-8
Identity = 24/60 (40.00%), Postives = 39/60 (65.00%), Query Frame = 3 
Query:    9 FHKMFVQIDTNKNGTISSKELDAALKKSG---DESERESIMKSLDLDGDSNITYAEYCTA 179
            + K F  +DT+ NGTI+++EL AALK +G    E++   ++  +D DGD  I++ E+ TA
Sbjct:   13 YKKAFSAVDTDGNGTINAQELGAALKATGKNLSEAQLRKLISEVDSDGDGEISFQEFLTA 72
[Close]
BLAST of SMED30029781 vs. Ensembl Celegans
Match: cal-1 (Calmodulin-like protein [Source:UniProtKB/Swiss-Prot;Acc:P04630])

HSP 1 Score: 46.2098 bits (108), Expect = 3.182e-7
Identity = 21/63 (33.33%), Postives = 37/63 (58.73%), Query Frame = 3 
Query:    6 DFHKMFVQIDTNKNGTISSKELDAALKKSGD---ESERESIMKSLDLDGDSNITYAEYCTALQ 185
            +F + F+  D + NGTIS+KEL  A++  G    E E   ++  +D+DG+  I + E+C  ++
Sbjct:   25 EFREAFMMFDKDGNGTISTKELGIAMRSLGQNPTEQEILEMINEVDIDGNGQIEFPEFCVMMK 87
[Close]
BLAST of SMED30029781 vs. Ensembl Celegans
Match: cal-1 (Calmodulin-like protein [Source:UniProtKB/Swiss-Prot;Acc:P04630])

HSP 1 Score: 46.2098 bits (108), Expect = 3.663e-7
Identity = 21/63 (33.33%), Postives = 37/63 (58.73%), Query Frame = 3 
Query:    6 DFHKMFVQIDTNKNGTISSKELDAALKKSGD---ESERESIMKSLDLDGDSNITYAEYCTALQ 185
            +F + F+  D + NGTIS+KEL  A++  G    E E   ++  +D+DG+  I + E+C  ++
Sbjct:   44 EFREAFMMFDKDGNGTISTKELGIAMRSLGQNPTEQEILEMINEVDIDGNGQIEFPEFCVMMK 106
[Close]
BLAST of SMED30029781 vs. Ensembl Celegans
Match: cal-3 (CALmodulin related genes [Source:UniProtKB/TrEMBL;Acc:U4PQW9])

HSP 1 Score: 45.0542 bits (105), Expect = 8.425e-7
Identity = 21/63 (33.33%), Postives = 36/63 (57.14%), Query Frame = 3 
Query:    6 DFHKMFVQIDTNKNGTISSKELDAALKKSGD---ESERESIMKSLDLDGDSNITYAEYCTALQ 185
            +F + F+  D + NGTIS KEL  A++  G    E +   I+  +DLDG+  + + E+C  ++
Sbjct:   30 EFKEAFLLFDKDGNGTISIKELGVAMRALGQNPTEQQMMEIIHDVDLDGNGQVEFPEFCVMMK 92
[Close]
BLAST of SMED30029781 vs. Ensembl Celegans
Match: cal-3 (CALmodulin related genes [Source:UniProtKB/TrEMBL;Acc:U4PQW9])

HSP 1 Score: 45.0542 bits (105), Expect = 1.311e-6
Identity = 21/63 (33.33%), Postives = 36/63 (57.14%), Query Frame = 3 
Query:    6 DFHKMFVQIDTNKNGTISSKELDAALKKSGD---ESERESIMKSLDLDGDSNITYAEYCTALQ 185
            +F + F+  D + NGTIS KEL  A++  G    E +   I+  +DLDG+  + + E+C  ++
Sbjct:  100 EFKEAFLLFDKDGNGTISIKELGVAMRALGQNPTEQQMMEIIHDVDLDGNGQVEFPEFCVMMK 162
[Close]
BLAST of SMED30029781 vs. UniProt/SwissProt
Match: sp|Q9NZT1|CALL5_HUMAN (Calmodulin-like protein 5 OS=Homo sapiens OX=9606 GN=CALML5 PE=1 SV=2)

HSP 1 Score: 50.447 bits (119), Expect = 8.966e-8
Identity = 24/60 (40.00%), Postives = 39/60 (65.00%), Query Frame = 3 
Query:    9 FHKMFVQIDTNKNGTISSKELDAALKKSG---DESERESIMKSLDLDGDSNITYAEYCTA 179
            + K F  +DT+ NGTI+++EL AALK +G    E++   ++  +D DGD  I++ E+ TA
Sbjct:   13 YKKAFSAVDTDGNGTINAQELGAALKATGKNLSEAQLRKLISEVDSDGDGEISFQEFLTA 72
[Close]
BLAST of SMED30029781 vs. UniProt/SwissProt
Match: sp|P49101|CDPK2_MAIZE (Calcium-dependent protein kinase 2 OS=Zea mays OX=4577 GN=CPK2 PE=2 SV=1)

HSP 1 Score: 48.9062 bits (115), Expect = 1.045e-6
Identity = 24/58 (41.38%), Postives = 40/58 (68.97%), Query Frame = 3 
Query:   15 KMFVQIDTNKNGTISSKELDAALKKSG---DESERESIMKSLDLDGDSNITYAEYCTA 179
            +MF+ +DT+ +GTI+ +EL A L K G    E+E + +M++ D+DG+ +I Y E+ TA
Sbjct:  373 QMFMNMDTDNSGTITYEELKAGLAKLGSKLSEAEVKQLMEAADVDGNGSIDYVEFITA 430
[Close]
BLAST of SMED30029781 vs. UniProt/SwissProt
Match: sp|P53683|CDPKJ_ORYSJ (Calcium-dependent protein kinase 19 OS=Oryza sativa subsp. japonica OX=39947 GN=CPK19 PE=2 SV=2)

HSP 1 Score: 48.521 bits (114), Expect = 1.594e-6
Identity = 24/58 (41.38%), Postives = 39/58 (67.24%), Query Frame = 3 
Query:   15 KMFVQIDTNKNGTISSKELDAALKKSG---DESERESIMKSLDLDGDSNITYAEYCTA 179
            +MF  +DT+ +GTI+ +EL A L K G    E+E + +M++ D+DG+ +I Y E+ TA
Sbjct:  392 QMFTNMDTDNSGTITYEELKAGLAKLGSKLSEAEVKQLMEAADVDGNGSIDYVEFITA 449
[Close]
BLAST of SMED30029781 vs. UniProt/SwissProt
Match: sp|P04630|CALL_CAEEL (Calmodulin-like protein OS=Caenorhabditis elegans OX=6239 GN=cal-1 PE=3 SV=1)

HSP 1 Score: 46.2098 bits (108), Expect = 4.048e-6
Identity = 21/63 (33.33%), Postives = 37/63 (58.73%), Query Frame = 3 
Query:    6 DFHKMFVQIDTNKNGTISSKELDAALKKSGD---ESERESIMKSLDLDGDSNITYAEYCTALQ 185
            +F + F+  D + NGTIS+KEL  A++  G    E E   ++  +D+DG+  I + E+C  ++
Sbjct:   25 EFREAFMMFDKDGNGTISTKELGIAMRSLGQNPTEQEILEMINEVDIDGNGQIEFPEFCVMMK 87
[Close]
BLAST of SMED30029781 vs. UniProt/SwissProt
Match: sp|Q9ZSA2|CDPKL_ARATH (Calcium-dependent protein kinase 21 OS=Arabidopsis thaliana OX=3702 GN=CPK21 PE=1 SV=1)

HSP 1 Score: 46.595 bits (109), Expect = 5.638e-6
Identity = 23/57 (40.35%), Postives = 37/57 (64.91%), Query Frame = 3 
Query:   18 MFVQIDTNKNGTISSKELDAALKKSG---DESERESIMKSLDLDGDSNITYAEYCTA 179
            MF  IDT+K+GTI+ +EL   L + G    E+E + +M++ D+DG+  I Y E+ +A
Sbjct:  388 MFANIDTDKSGTITYEELKTGLTRLGSRLSETEVKQLMEAADVDGNGTIDYYEFISA 444
[Close]
BLAST of SMED30029781 vs. TrEMBL
Match: A0A183W589 (Uncharacterized protein OS=Trichobilharzia regenti OX=157069 GN=TRE_LOCUS7545 PE=4 SV=1)

HSP 1 Score: 51.6026 bits (122), Expect = 2.407e-6
Identity = 26/67 (38.81%), Postives = 41/67 (61.19%), Query Frame = 3 
Query:    6 DFHKMFVQIDTNKNGTISSKELDAALKKSGDESER-ESIMKSLDLDGDSNITYAEYCTALQLKPDPA 203
            +  ++F  +DTN +G +S +EL   L  +G  + R + +MK LD++GD  IT  EY +AL L  +PA
Sbjct:    9 ELMELFKSLDTNGDGVVSRQELTTRLANAGVSARRVQDLMKHLDINGDGFITVEEYKSALGLTKEPA 75
[Close]
BLAST of SMED30029781 vs. TrEMBL
Match: G7YG42 (16 kDa calcium-binding protein OS=Clonorchis sinensis OX=79923 GN=CLF_107067 PE=4 SV=1)

HSP 1 Score: 51.6026 bits (122), Expect = 8.753e-6
Identity = 29/66 (43.94%), Postives = 38/66 (57.58%), Query Frame = 3 
Query:    9 FHKMFVQIDTNKNGTISSKELDAALKKSG-DESERESIMKSLDLDGDSNITYAEYCTALQLKPDPA 203
            FH M    DT++NG +S KE+ A ++ SG   S  +  M   DLDGD  +T  EY TAL L P P+
Sbjct:   15 FHAM----DTDRNGVLSLKEVRACVENSGLPPSAVDEFMSLFDLDGDRKVTLNEYITALGLNPPPS 76
[Close]
BLAST of SMED30029781 vs. TrEMBL
Match: A0A0N5C147 (Uncharacterized protein OS=Strongyloides papillosus OX=174720 PE=4 SV=1)

HSP 1 Score: 52.373 bits (124), Expect = 8.833e-6
Identity = 22/63 (34.92%), Postives = 39/63 (61.90%), Query Frame = 3 
Query:    6 DFHKMFVQIDTNKNGTISSKELDAALKKSGD---ESERESIMKSLDLDGDSNITYAEYCTALQ 185
            +FH+ F+  D ++NGTIS+KEL  A++  G    E E   ++  +D+DG+  I + E+C  ++
Sbjct:   50 EFHEAFIMFDKDRNGTISTKELGVAMRSLGQNPTEQEILEMINEVDIDGNGQIEFPEFCVMMK 112
[Close]
BLAST of SMED30029781 vs. Ensembl Cavefish
Match: cabp7b (calcium-binding protein 7 [Source:NCBI gene;Acc:103046176])

HSP 1 Score: 44.2838 bits (103), Expect = 3.538e-6
Identity = 23/57 (40.35%), Postives = 33/57 (57.89%), Query Frame = 3 
Query:   21 FVQIDTNKNGTISSKELDAALKKSG---DESERESIMKSLDLDGDSNITYAEYCTAL 182
            F   D + NG IS +EL  A++  G   +E E E I+K LD+DGD  + + E+ T L
Sbjct:   42 FKVFDRDGNGFISKQELGVAMRSLGYMPNEVELEVIIKRLDMDGDGQVGFEEFVTLL 98
[Close]
BLAST of SMED30029781 vs. Ensembl Yeast
Match: CMD1 (Calmodulin; Ca2+ binding protein that regulates Ca2+ independent processes (mitosis, bud growth, actin organization, endocytosis, etc.) and Ca2+ dependent processes (stress-activated pathways), targets include Nuf1p, Myo2p and calcineurin; binds to the Hog1p MAPK in response to hyperosmotic stress; potentiates membrane tubulation and constriction mediated by the Rvs161p-Rvs167p complex; human CALM1 or CALM2 functionally complement repression induced inviability [Source:SGD;Acc:S000000313])

HSP 1 Score: 40.817 bits (94), Expect = 5.830e-6
Identity = 22/69 (31.88%), Postives = 38/69 (55.07%), Query Frame = 3 
Query:    6 DFHKMFVQIDTNKNGTISSKELDAALKKSG---DESERESIMKSLDLDGDSNITYAEYCTAL--QLKPD 197
            +F + F   D + NG+ISS EL   ++  G    E+E   +M  +D+DG+  I ++E+   +  QLK +
Sbjct:   12 EFKEAFALFDKDNNGSISSSELATVMRSLGLSPSEAEVNDLMNEIDVDGNHQIEFSEFLALMSRQLKSN 80
[Close]
BLAST of SMED30029781 vs. Planmine SMEST
Match: SMESG000018602.1 (SMESG000018602.1)

HSP 1 Score: 68.9366 bits (167), Expect = 8.656e-17
Identity = 33/70 (47.14%), Postives = 47/70 (67.14%), Query Frame = 3 
Query:    3 MDFHKMFVQIDTNKNGTISSKELDAALKKSGDESERESIMKSLDLDGDSNITYAEYCTALQLKPDPAIVK 212
            MD H++FV+ID +KNG I+ KE D A K  G E   E + K  D +G S+ITYA+YC+A+ + P+ +I K
Sbjct:    1 MDRHQIFVKIDHDKNGLITKKEFDEAQKSFGKEETIEILKKCFDSNGGSSITYADYCSAVGISPNASIQK 70
[Close]
BLAST of SMED30029781 vs. Planmine SMEST
Match: SMESG000018597.1 (SMESG000018597.1)

HSP 1 Score: 68.1662 bits (165), Expect = 1.554e-16
Identity = 31/61 (50.82%), Postives = 44/61 (72.13%), Query Frame = 3 
Query:   30 IDTNKNGTISSKELDAALKKSGDESERESIMKSLDLDGDSNITYAEYCTALQLKPDPAIVK 212
            ID + +G+IS  EL +ALK+SG+E   + I+  LD++GD  ITY EYC+AL L PDP +V+
Sbjct:    2 IDQDSSGSISINELKSALKQSGEEFLMDEILNGLDINGDGEITYMEYCSALDLIPDPGLVR 62
[Close]
BLAST of SMED30029781 vs. Planmine SMEST
Match: SMESG000018598.1 (SMESG000018598.1)

HSP 1 Score: 60.4622 bits (145), Expect = 2.254e-13
Identity = 30/70 (42.86%), Postives = 45/70 (64.29%), Query Frame = 3 
Query:    3 MDFHKMFVQIDTNKNGTISSKELDAALKKSGDESERESIMKSLDLDGDSNITYAEYCTALQLKPDPAIVK 212
            MD H  F++ID+NKNG I+ KE + A KKS  +++ E+I K  + +  +NITYAE+C    + PD   +K
Sbjct:    1 MDHHATFLKIDSNKNGIITLKEFEDA-KKSIGKADAEAIRKQFESNSGNNITYAEFCNLFGIAPDDGTMK 69
[Close]
BLAST of SMED30029781 vs. Planmine SMEST
Match: SMESG000002907.1 (SMESG000002907.1)

HSP 1 Score: 49.6766 bits (117), Expect = 3.173e-9
Identity = 26/59 (44.07%), Postives = 37/59 (62.71%), Query Frame = 3 
Query:   15 KMFVQIDTNKNGTISSKELDAALKKSGDESER-ESIMKSLDLDGDSNITYAEYCTALQL 188
            KMF++IDTNKNG IS  EL     K+ +  E  ++ +K+ D D D N+TY E+  AL +
Sbjct:    8 KMFLKIDTNKNGVISIDELKQHCSKTANVDEAIKTFLKNFDKDKDKNVTYEEFAKALGI 66
[Close]
BLAST of SMED30029781 vs. Planmine SMEST
Match: SMESG000048475.1 (SMESG000048475.1)

HSP 1 Score: 43.5134 bits (101), Expect = 8.690e-7
Identity = 24/57 (42.11%), Postives = 32/57 (56.14%), Query Frame = 3 
Query:   15 KMFVQIDTNKNGTISSKELDAALKKSGDESER-ESIMKSLDLDGDSNITYAEYCTAL 182
            KMF+ +D NKNG IS +EL     K  +  E  E  +K+ D + D  ITY E+  AL
Sbjct:    8 KMFLAVDINKNGIISVEELRKHCSKCSNVDEAVEKFLKNFDTNRDREITYKEFADAL 64
The following BLAST results are available for this feature:
BLAST of SMED30029781 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 1
Match NameE-valueIdentityDescription
CALML51.867e-840.00calmodulin like 5 [Source:HGNC Symbol;Acc:HGNC:181... [more]
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BLAST of SMED30029781 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 4
Match NameE-valueIdentityDescription
cal-13.182e-733.33Calmodulin-like protein [Source:UniProtKB/Swiss-P... [more]
cal-13.663e-733.33Calmodulin-like protein [Source:UniProtKB/Swiss-P... [more]
cal-38.425e-733.33CALmodulin related genes [Source:UniProtKB/TrEMBL... [more]
cal-31.311e-633.33CALmodulin related genes [Source:UniProtKB/TrEMBL... [more]
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BLAST of SMED30029781 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30029781 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30029781 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30029781 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30029781 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q9NZT1|CALL5_HUMAN8.966e-840.00Calmodulin-like protein 5 OS=Homo sapiens OX=9606 ... [more]
sp|P49101|CDPK2_MAIZE1.045e-641.38Calcium-dependent protein kinase 2 OS=Zea mays OX=... [more]
sp|P53683|CDPKJ_ORYSJ1.594e-641.38Calcium-dependent protein kinase 19 OS=Oryza sativ... [more]
sp|P04630|CALL_CAEEL4.048e-633.33Calmodulin-like protein OS=Caenorhabditis elegans ... [more]
sp|Q9ZSA2|CDPKL_ARATH5.638e-640.35Calcium-dependent protein kinase 21 OS=Arabidopsis... [more]
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BLAST of SMED30029781 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 3
Match NameE-valueIdentityDescription
A0A183W5892.407e-638.81Uncharacterized protein OS=Trichobilharzia regenti... [more]
G7YG428.753e-643.9416 kDa calcium-binding protein OS=Clonorchis sinen... [more]
A0A0N5C1478.833e-634.92Uncharacterized protein OS=Strongyloides papillosu... [more]
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BLAST of SMED30029781 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
cabp7b3.538e-640.35calcium-binding protein 7 [Source:NCBI gene;Acc:10... [more]
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BLAST of SMED30029781 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30029781 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1
Match NameE-valueIdentityDescription
CMD15.830e-631.88Calmodulin; Ca2+ binding protein that regulates Ca... [more]
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BLAST of SMED30029781 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30029781 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30029781 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000018602.18.656e-1747.14SMESG000018602.1[more]
SMESG000018597.11.554e-1650.82SMESG000018597.1[more]
SMESG000018598.12.254e-1342.86SMESG000018598.1[more]
SMESG000002907.13.173e-944.07SMESG000002907.1[more]
SMESG000048475.18.690e-742.11SMESG000048475.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30029781 ID=SMED30029781|Name=SMED30029781|organism=Schmidtea mediterranea sexual|type=transcript|length=323bp
AAATGGACTTTCACAAAATGTTTGTACAAATTGACACCAATAAAAATGGT
ACAATTTCATCAAAAGAATTAGATGCGGCTTTGAAGAAAAGTGGCGATGA
AAGCGAACGAGAATCAATTATGAAATCTCTAGACCTTGATGGGGACAGCA
ATATCACCTATGCCGAATATTGTACTGCTCTTCAATTGAAACCAGATCCA
GCAATTGTGAAAGGTTTTAAGAAGTAAATGAACAATTTTTAGTATTTTTT
TTTCGTATAAGATGATGATAATTTAGGTAGAATTTGGTTTTTGTAGAAAA
CGTTTTATTTAATAGAATAAAAG
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000020protonephridia
PLANA:0000110pharynx progenitor cell
PLANA:0000420parapharyngeal region
Vocabulary: INTERPRO
TermDefinition
IPR002048EF_hand_dom
IPR011992EF-hand-dom_pair
IPR018247EF_Hand_1_Ca_BS
Vocabulary: molecular function
TermDefinition
GO:0005509calcium ion binding
GO:0000166nucleotide binding
GO:0004672protein kinase activity
GO:0004674protein serine/threonine kinase activity
GO:0005524ATP binding
GO:0016301kinase activity
GO:0016740transferase activity
GO:0046872metal ion binding
Vocabulary: biological process
TermDefinition
GO:0006468protein phosphorylation
GO:0016310phosphorylation
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainSMARTSM00054efh_1coord: 35..63
e-value: 2.1
score: 15.2
coord: 2..30
e-value: 0.0011
score: 28.2
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 3..60
e-value: 1.6E-10
score: 41.2
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 37..66
score: 8.823
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 1..33
score: 12.338
IPR002048EF-hand domainCDDcd00051EFhcoord: 2..61
e-value: 8.25708E-11
score: 49.8537
NoneNo IPR availableGENE3DG3DSA:1.10.238.10coord: 1..67
e-value: 4.1E-14
score: 54.6
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 11..23
IPR011992EF-hand domain pairSUPERFAMILYSSF47473EF-handcoord: 2..63