SMED30029781
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30029781 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 8
Alignments
SMED30029781 aligns in the following genomic locations:
Homology
BLAST of SMED30029781 vs. Ensembl Human
Match: CALML5 (calmodulin like 5 [Source:HGNC Symbol;Acc:HGNC:18180]) HSP 1 Score: 50.447 bits (119), Expect = 1.867e-8 Identity = 24/60 (40.00%), Postives = 39/60 (65.00%), Query Frame = 3 Query: 9 FHKMFVQIDTNKNGTISSKELDAALKKSG---DESERESIMKSLDLDGDSNITYAEYCTA 179 + K F +DT+ NGTI+++EL AALK +G E++ ++ +D DGD I++ E+ TA Sbjct: 13 YKKAFSAVDTDGNGTINAQELGAALKATGKNLSEAQLRKLISEVDSDGDGEISFQEFLTA 72
BLAST of SMED30029781 vs. Ensembl Celegans
Match: cal-1 (Calmodulin-like protein [Source:UniProtKB/Swiss-Prot;Acc:P04630]) HSP 1 Score: 46.2098 bits (108), Expect = 3.182e-7 Identity = 21/63 (33.33%), Postives = 37/63 (58.73%), Query Frame = 3 Query: 6 DFHKMFVQIDTNKNGTISSKELDAALKKSGD---ESERESIMKSLDLDGDSNITYAEYCTALQ 185 +F + F+ D + NGTIS+KEL A++ G E E ++ +D+DG+ I + E+C ++ Sbjct: 25 EFREAFMMFDKDGNGTISTKELGIAMRSLGQNPTEQEILEMINEVDIDGNGQIEFPEFCVMMK 87
BLAST of SMED30029781 vs. Ensembl Celegans
Match: cal-1 (Calmodulin-like protein [Source:UniProtKB/Swiss-Prot;Acc:P04630]) HSP 1 Score: 46.2098 bits (108), Expect = 3.663e-7 Identity = 21/63 (33.33%), Postives = 37/63 (58.73%), Query Frame = 3 Query: 6 DFHKMFVQIDTNKNGTISSKELDAALKKSGD---ESERESIMKSLDLDGDSNITYAEYCTALQ 185 +F + F+ D + NGTIS+KEL A++ G E E ++ +D+DG+ I + E+C ++ Sbjct: 44 EFREAFMMFDKDGNGTISTKELGIAMRSLGQNPTEQEILEMINEVDIDGNGQIEFPEFCVMMK 106
BLAST of SMED30029781 vs. Ensembl Celegans
Match: cal-3 (CALmodulin related genes [Source:UniProtKB/TrEMBL;Acc:U4PQW9]) HSP 1 Score: 45.0542 bits (105), Expect = 8.425e-7 Identity = 21/63 (33.33%), Postives = 36/63 (57.14%), Query Frame = 3 Query: 6 DFHKMFVQIDTNKNGTISSKELDAALKKSGD---ESERESIMKSLDLDGDSNITYAEYCTALQ 185 +F + F+ D + NGTIS KEL A++ G E + I+ +DLDG+ + + E+C ++ Sbjct: 30 EFKEAFLLFDKDGNGTISIKELGVAMRALGQNPTEQQMMEIIHDVDLDGNGQVEFPEFCVMMK 92
BLAST of SMED30029781 vs. Ensembl Celegans
Match: cal-3 (CALmodulin related genes [Source:UniProtKB/TrEMBL;Acc:U4PQW9]) HSP 1 Score: 45.0542 bits (105), Expect = 1.311e-6 Identity = 21/63 (33.33%), Postives = 36/63 (57.14%), Query Frame = 3 Query: 6 DFHKMFVQIDTNKNGTISSKELDAALKKSGD---ESERESIMKSLDLDGDSNITYAEYCTALQ 185 +F + F+ D + NGTIS KEL A++ G E + I+ +DLDG+ + + E+C ++ Sbjct: 100 EFKEAFLLFDKDGNGTISIKELGVAMRALGQNPTEQQMMEIIHDVDLDGNGQVEFPEFCVMMK 162
BLAST of SMED30029781 vs. UniProt/SwissProt
Match: sp|Q9NZT1|CALL5_HUMAN (Calmodulin-like protein 5 OS=Homo sapiens OX=9606 GN=CALML5 PE=1 SV=2) HSP 1 Score: 50.447 bits (119), Expect = 8.966e-8 Identity = 24/60 (40.00%), Postives = 39/60 (65.00%), Query Frame = 3 Query: 9 FHKMFVQIDTNKNGTISSKELDAALKKSG---DESERESIMKSLDLDGDSNITYAEYCTA 179 + K F +DT+ NGTI+++EL AALK +G E++ ++ +D DGD I++ E+ TA Sbjct: 13 YKKAFSAVDTDGNGTINAQELGAALKATGKNLSEAQLRKLISEVDSDGDGEISFQEFLTA 72
BLAST of SMED30029781 vs. UniProt/SwissProt
Match: sp|P49101|CDPK2_MAIZE (Calcium-dependent protein kinase 2 OS=Zea mays OX=4577 GN=CPK2 PE=2 SV=1) HSP 1 Score: 48.9062 bits (115), Expect = 1.045e-6 Identity = 24/58 (41.38%), Postives = 40/58 (68.97%), Query Frame = 3 Query: 15 KMFVQIDTNKNGTISSKELDAALKKSG---DESERESIMKSLDLDGDSNITYAEYCTA 179 +MF+ +DT+ +GTI+ +EL A L K G E+E + +M++ D+DG+ +I Y E+ TA Sbjct: 373 QMFMNMDTDNSGTITYEELKAGLAKLGSKLSEAEVKQLMEAADVDGNGSIDYVEFITA 430
BLAST of SMED30029781 vs. UniProt/SwissProt
Match: sp|P53683|CDPKJ_ORYSJ (Calcium-dependent protein kinase 19 OS=Oryza sativa subsp. japonica OX=39947 GN=CPK19 PE=2 SV=2) HSP 1 Score: 48.521 bits (114), Expect = 1.594e-6 Identity = 24/58 (41.38%), Postives = 39/58 (67.24%), Query Frame = 3 Query: 15 KMFVQIDTNKNGTISSKELDAALKKSG---DESERESIMKSLDLDGDSNITYAEYCTA 179 +MF +DT+ +GTI+ +EL A L K G E+E + +M++ D+DG+ +I Y E+ TA Sbjct: 392 QMFTNMDTDNSGTITYEELKAGLAKLGSKLSEAEVKQLMEAADVDGNGSIDYVEFITA 449
BLAST of SMED30029781 vs. UniProt/SwissProt
Match: sp|P04630|CALL_CAEEL (Calmodulin-like protein OS=Caenorhabditis elegans OX=6239 GN=cal-1 PE=3 SV=1) HSP 1 Score: 46.2098 bits (108), Expect = 4.048e-6 Identity = 21/63 (33.33%), Postives = 37/63 (58.73%), Query Frame = 3 Query: 6 DFHKMFVQIDTNKNGTISSKELDAALKKSGD---ESERESIMKSLDLDGDSNITYAEYCTALQ 185 +F + F+ D + NGTIS+KEL A++ G E E ++ +D+DG+ I + E+C ++ Sbjct: 25 EFREAFMMFDKDGNGTISTKELGIAMRSLGQNPTEQEILEMINEVDIDGNGQIEFPEFCVMMK 87
BLAST of SMED30029781 vs. UniProt/SwissProt
Match: sp|Q9ZSA2|CDPKL_ARATH (Calcium-dependent protein kinase 21 OS=Arabidopsis thaliana OX=3702 GN=CPK21 PE=1 SV=1) HSP 1 Score: 46.595 bits (109), Expect = 5.638e-6 Identity = 23/57 (40.35%), Postives = 37/57 (64.91%), Query Frame = 3 Query: 18 MFVQIDTNKNGTISSKELDAALKKSG---DESERESIMKSLDLDGDSNITYAEYCTA 179 MF IDT+K+GTI+ +EL L + G E+E + +M++ D+DG+ I Y E+ +A Sbjct: 388 MFANIDTDKSGTITYEELKTGLTRLGSRLSETEVKQLMEAADVDGNGTIDYYEFISA 444
BLAST of SMED30029781 vs. TrEMBL
Match: A0A183W589 (Uncharacterized protein OS=Trichobilharzia regenti OX=157069 GN=TRE_LOCUS7545 PE=4 SV=1) HSP 1 Score: 51.6026 bits (122), Expect = 2.407e-6 Identity = 26/67 (38.81%), Postives = 41/67 (61.19%), Query Frame = 3 Query: 6 DFHKMFVQIDTNKNGTISSKELDAALKKSGDESER-ESIMKSLDLDGDSNITYAEYCTALQLKPDPA 203 + ++F +DTN +G +S +EL L +G + R + +MK LD++GD IT EY +AL L +PA Sbjct: 9 ELMELFKSLDTNGDGVVSRQELTTRLANAGVSARRVQDLMKHLDINGDGFITVEEYKSALGLTKEPA 75
BLAST of SMED30029781 vs. TrEMBL
Match: G7YG42 (16 kDa calcium-binding protein OS=Clonorchis sinensis OX=79923 GN=CLF_107067 PE=4 SV=1) HSP 1 Score: 51.6026 bits (122), Expect = 8.753e-6 Identity = 29/66 (43.94%), Postives = 38/66 (57.58%), Query Frame = 3 Query: 9 FHKMFVQIDTNKNGTISSKELDAALKKSG-DESERESIMKSLDLDGDSNITYAEYCTALQLKPDPA 203 FH M DT++NG +S KE+ A ++ SG S + M DLDGD +T EY TAL L P P+ Sbjct: 15 FHAM----DTDRNGVLSLKEVRACVENSGLPPSAVDEFMSLFDLDGDRKVTLNEYITALGLNPPPS 76
BLAST of SMED30029781 vs. TrEMBL
Match: A0A0N5C147 (Uncharacterized protein OS=Strongyloides papillosus OX=174720 PE=4 SV=1) HSP 1 Score: 52.373 bits (124), Expect = 8.833e-6 Identity = 22/63 (34.92%), Postives = 39/63 (61.90%), Query Frame = 3 Query: 6 DFHKMFVQIDTNKNGTISSKELDAALKKSGD---ESERESIMKSLDLDGDSNITYAEYCTALQ 185 +FH+ F+ D ++NGTIS+KEL A++ G E E ++ +D+DG+ I + E+C ++ Sbjct: 50 EFHEAFIMFDKDRNGTISTKELGVAMRSLGQNPTEQEILEMINEVDIDGNGQIEFPEFCVMMK 112
BLAST of SMED30029781 vs. Ensembl Cavefish
Match: cabp7b (calcium-binding protein 7 [Source:NCBI gene;Acc:103046176]) HSP 1 Score: 44.2838 bits (103), Expect = 3.538e-6 Identity = 23/57 (40.35%), Postives = 33/57 (57.89%), Query Frame = 3 Query: 21 FVQIDTNKNGTISSKELDAALKKSG---DESERESIMKSLDLDGDSNITYAEYCTAL 182 F D + NG IS +EL A++ G +E E E I+K LD+DGD + + E+ T L Sbjct: 42 FKVFDRDGNGFISKQELGVAMRSLGYMPNEVELEVIIKRLDMDGDGQVGFEEFVTLL 98
BLAST of SMED30029781 vs. Ensembl Yeast
Match: CMD1 (Calmodulin; Ca2+ binding protein that regulates Ca2+ independent processes (mitosis, bud growth, actin organization, endocytosis, etc.) and Ca2+ dependent processes (stress-activated pathways), targets include Nuf1p, Myo2p and calcineurin; binds to the Hog1p MAPK in response to hyperosmotic stress; potentiates membrane tubulation and constriction mediated by the Rvs161p-Rvs167p complex; human CALM1 or CALM2 functionally complement repression induced inviability [Source:SGD;Acc:S000000313]) HSP 1 Score: 40.817 bits (94), Expect = 5.830e-6 Identity = 22/69 (31.88%), Postives = 38/69 (55.07%), Query Frame = 3 Query: 6 DFHKMFVQIDTNKNGTISSKELDAALKKSG---DESERESIMKSLDLDGDSNITYAEYCTAL--QLKPD 197 +F + F D + NG+ISS EL ++ G E+E +M +D+DG+ I ++E+ + QLK + Sbjct: 12 EFKEAFALFDKDNNGSISSSELATVMRSLGLSPSEAEVNDLMNEIDVDGNHQIEFSEFLALMSRQLKSN 80
BLAST of SMED30029781 vs. Planmine SMEST
Match: SMESG000018602.1 (SMESG000018602.1) HSP 1 Score: 68.9366 bits (167), Expect = 8.656e-17 Identity = 33/70 (47.14%), Postives = 47/70 (67.14%), Query Frame = 3 Query: 3 MDFHKMFVQIDTNKNGTISSKELDAALKKSGDESERESIMKSLDLDGDSNITYAEYCTALQLKPDPAIVK 212 MD H++FV+ID +KNG I+ KE D A K G E E + K D +G S+ITYA+YC+A+ + P+ +I K Sbjct: 1 MDRHQIFVKIDHDKNGLITKKEFDEAQKSFGKEETIEILKKCFDSNGGSSITYADYCSAVGISPNASIQK 70
BLAST of SMED30029781 vs. Planmine SMEST
Match: SMESG000018597.1 (SMESG000018597.1) HSP 1 Score: 68.1662 bits (165), Expect = 1.554e-16 Identity = 31/61 (50.82%), Postives = 44/61 (72.13%), Query Frame = 3 Query: 30 IDTNKNGTISSKELDAALKKSGDESERESIMKSLDLDGDSNITYAEYCTALQLKPDPAIVK 212 ID + +G+IS EL +ALK+SG+E + I+ LD++GD ITY EYC+AL L PDP +V+ Sbjct: 2 IDQDSSGSISINELKSALKQSGEEFLMDEILNGLDINGDGEITYMEYCSALDLIPDPGLVR 62
BLAST of SMED30029781 vs. Planmine SMEST
Match: SMESG000018598.1 (SMESG000018598.1) HSP 1 Score: 60.4622 bits (145), Expect = 2.254e-13 Identity = 30/70 (42.86%), Postives = 45/70 (64.29%), Query Frame = 3 Query: 3 MDFHKMFVQIDTNKNGTISSKELDAALKKSGDESERESIMKSLDLDGDSNITYAEYCTALQLKPDPAIVK 212 MD H F++ID+NKNG I+ KE + A KKS +++ E+I K + + +NITYAE+C + PD +K Sbjct: 1 MDHHATFLKIDSNKNGIITLKEFEDA-KKSIGKADAEAIRKQFESNSGNNITYAEFCNLFGIAPDDGTMK 69
BLAST of SMED30029781 vs. Planmine SMEST
Match: SMESG000002907.1 (SMESG000002907.1) HSP 1 Score: 49.6766 bits (117), Expect = 3.173e-9 Identity = 26/59 (44.07%), Postives = 37/59 (62.71%), Query Frame = 3 Query: 15 KMFVQIDTNKNGTISSKELDAALKKSGDESER-ESIMKSLDLDGDSNITYAEYCTALQL 188 KMF++IDTNKNG IS EL K+ + E ++ +K+ D D D N+TY E+ AL + Sbjct: 8 KMFLKIDTNKNGVISIDELKQHCSKTANVDEAIKTFLKNFDKDKDKNVTYEEFAKALGI 66
BLAST of SMED30029781 vs. Planmine SMEST
Match: SMESG000048475.1 (SMESG000048475.1) HSP 1 Score: 43.5134 bits (101), Expect = 8.690e-7 Identity = 24/57 (42.11%), Postives = 32/57 (56.14%), Query Frame = 3 Query: 15 KMFVQIDTNKNGTISSKELDAALKKSGDESER-ESIMKSLDLDGDSNITYAEYCTAL 182 KMF+ +D NKNG IS +EL K + E E +K+ D + D ITY E+ AL Sbjct: 8 KMFLAVDINKNGIISVEELRKHCSKCSNVDEAVEKFLKNFDTNRDREITYKEFADAL 64 The following BLAST results are available for this feature:
BLAST of SMED30029781 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 1
BLAST of SMED30029781 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 4
BLAST of SMED30029781 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30029781 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30029781 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30029781 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30029781 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of SMED30029781 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 3
BLAST of SMED30029781 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 1
BLAST of SMED30029781 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30029781 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 1
BLAST of SMED30029781 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30029781 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30029781 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30029781 ID=SMED30029781|Name=SMED30029781|organism=Schmidtea mediterranea sexual|type=transcript|length=323bpback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
|