SMED30029771
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30029771 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 29Note: Hover over icons to view figure legend
Alignments
SMED30029771 aligns in the following genomic locations:
Homology
BLAST of SMED30029771 vs. Planmine SMEST
Match: SMESG000075916.1 (SMESG000075916.1) HSP 1 Score: 497.664 bits (1280), Expect = 3.503e-179 Identity = 289/293 (98.63%), Postives = 291/293 (99.32%), Query Frame = 2 Query: 2 MNIEQRFSCIKILVIIGFAFVSFSFTLAIATYSIPYWLTLSGSSNISSDWRSYSRSIGIFQECLITQITSTISIPAPYIIKNNCTDNDIGQYGVTSSKSYYLTIIRMRQSQIGLHGAXXXXXXXXXXXXXXXXMKWCCGFMDSLFYIKWRNXXXXXXXXXXXXXXXXXXIGTAFYLMYGDTTYQVNELSLISFQQGSVTVGFMFYLAWTGCLTSLLASICFFISSHYGKSLEMSKYKPHVISVDNPAFDSDDYKIAEPIFLQPSIGRNDFSKLSLTANVNNPGPLPRYNINVN 880 MNIEQRFSCIKILVIIGFAFVSFSFTLAIATYSIPYWLTLS SSNISSDWRSYSRSIGIFQECLITQITSTISIPAPYIIKNNCTDNDIGQYGVTSS SYYLTIIRMRQSQIGLHGATILLLLFVLLTGIGIFMKWCCGFMDSLFYIKWRNALGCLSLLSFLCFSASSSIGTAFYLMYG+TTYQV+ELSLISFQQGSVTVGFMFYLAWTGCLTSLLASICFFISSHYGKSLEMSKYKPHVISVDNPAFDSDDYKIAEPIFLQPSIGRNDFSKLSLTANVNNPGPLPRYNINVN Sbjct: 1 MNIEQRFSCIKILVIIGFAFVSFSFTLAIATYSIPYWLTLSSSSNISSDWRSYSRSIGIFQECLITQITSTISIPAPYIIKNNCTDNDIGQYGVTSSNSYYLTIIRMRQSQIGLHGATILLLLFVLLTGIGIFMKWCCGFMDSLFYIKWRNALGCLSLLSFLCFSASSSIGTAFYLMYGNTTYQVSELSLISFQQGSVTVGFMFYLAWTGCLTSLLASICFFISSHYGKSLEMSKYKPHVISVDNPAFDSDDYKIAEPIFLQPSIGRNDFSKLSLTANVNNPGPLPRYNINVN 293
BLAST of SMED30029771 vs. Planmine SMEST
Match: SMESG000064443.1 (SMESG000064443.1) HSP 1 Score: 149.058 bits (375), Expect = 2.230e-42 Identity = 112/299 (37.46%), Postives = 168/299 (56.19%), Query Frame = 2 Query: 2 MNIEQRFSCIKILVIIGFAFVSFSFTLAIATYSIPYWLT-LSGSSNISSDWRSYSRSIGIFQECLITQITSTISIPAPYII-KNNCTDNDIGQYGVTSSKSYYLTIIRMRQSQIGLHGAXXXXXXXXXXXXXXXXMKWCCGFMDSLFYIKWRNXXXXXXXXXXXXXXXXXXIGTA-FYLMYGDTTYQVNELSLISFQQGSVT-VGFMFYLAWTGCLTSLLASICFFISSHYGKSLEMSKYKPHVISVDNPAFDSDDYKIAEPIFLQ--PSIGRNDFSKLSLTANVNNPGPLPRYNINVN 880 MN E++ C+K+L+I GF S LA+ +Y IP W+ ++ SS++ S W Y+R+ GIFQEC+ + + ++ PY++ K C ND+GQY S Y IIRMRQS I LH +ILLLL +L+ GIG+F+KWCC D+++Y+KWR L S++ LC SAS SI T +Y + + Y+ N L F ++ G ++Y+AW + L+SI F +SS GK +E+ K+ P N AF +D++ + + Q I RN S P L RY+++ N Sbjct: 1 MNREKKLFCVKLLLIFGFTLAFLSCVLAVISYIIPQWMVYVAYSSDVPSKWIEYNRTRGIFQECMESTLKQDPAVQLPYVMNKQQCITNDVGQYNSDSGDGNYEIIIRMRQSHIALHSVSILLLLIMLIFGIGVFIKWCCMTDDTIYYLKWRITLSIFSIIICLCLSASCSIATVLYYEVEVNMKYKTNLLPFSQFNLNNIKRFGPIYYIAWISAGLAFLSSIFFTVSSCLGKEMEIEKFYPENWKKTNDAFLNDEFPKSNQFYEQFPHPIKRNLPSA---------PESLQRYDLSNN 290
BLAST of SMED30029771 vs. Planmine SMEST
Match: SMESG000064443.1 (SMESG000064443.1) HSP 1 Score: 118.242 bits (295), Expect = 3.440e-31 Identity = 96/256 (37.50%), Postives = 142/256 (55.47%), Query Frame = 2 Query: 128 SSNISSDWRSYSRSIGIFQECLITQITSTISIPAPYII-KNNCTDNDIGQYGVTSSKSYYLTIIRMRQSQIGLHGAXXXXXXXXXXXXXXXXMKWCCGFMDSLFYIKWRNXXXXXXXXXXXXXXXXXXIGTA-FYLMYGDTTYQVNELSLISFQQGSVT-VGFMFYLAWTGCLTSLLASICFFISSHYGKSLEMSKYKPHVISVDNPAFDSDDYKIAEPIFLQ--PSIGRNDFSKLSLTANVNNPGPLPRYNINVN 880 SS++ S W Y+R+ GIFQEC+ + + ++ PY++ K C ND+GQY S Y IIRMRQS I LH +ILLLL +L+ GIG+F+KWCC D+++Y+KWR L S++ LC SAS SI T +Y + + Y+ N L F ++ G ++Y+AW + L+SI F +SS GK +E+ K+ P N AF +D++ + + Q I RN S P L RY+++ N Sbjct: 7 SSDVPSKWIEYNRTRGIFQECMESTLKQDPAVQLPYVMNKQQCITNDVGQYNSDSGDGNYEIIIRMRQSHIALHSVSILLLLIMLIFGIGVFIKWCCMTDDTIYYLKWRITLSIFSIIICLCLSASCSIATVLYYEVEVNMKYKTNLLPFSQFNLNNIKRFGPIYYIAWISAGLAFLSSIFFTVSSCLGKEMEIEKFYPENWKKTNDAFLNDEFPKSNQFYEQFPHPIKRNLPSA---------PESLQRYDLSNN 253 The following BLAST results are available for this feature:
BLAST of SMED30029771 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30029771 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30029771 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30029771 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30029771 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30029771 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30029771 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30029771 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30029771 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30029771 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30029771 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30029771 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30029771 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30029771 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 3
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30029771 ID=SMED30029771|Name=SMED30029771|organism=Schmidtea mediterranea sexual|type=transcript|length=990bpback to top protein sequence of SMED30029771-orf-1 >SMED30029771-orf-1 ID=SMED30029771-orf-1|Name=SMED30029771-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=294bp MNIEQRFSCIKILVIIGFAFVSFSFTLAIATYSIPYWLTLSGSSNISSDWback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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