SMED30029741

Overview
NameSMED30029741
Smed IDSMED30029741
Length (bp)1439
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30029741 (SMED30029741) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30029741 (SMED30029741) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30029741 (SMED30029741) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30029741 (SMED30029741) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30029741

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 21

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30029741h1SMcG0006354 SmedASXL_010846SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30029741h1SMcG0006354 SmedASXL_010846SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
pharynxSMED30029741h1SMcG0006354 dd_Smed_v4_2250_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30029741h1SMcG0006354 dd_Smed_v4_2250_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30029741h1SMcG0006354 dd_Smed_v4_2250_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30029741h1SMcG0006354 dd_Smed_v4_2250_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30029741h1SMcG0006354 dd_Smed_v4_2250_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30029741h1SMcG0006354 dd_Smed_v4_2250_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30029741h1SMcG0006354 dd_Smed_v4_2250_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymaSMED30029741h1SMcG0006354 dd_Smed_v4_2250_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30029741h1SMcG0006354 dd_Smed_v4_2250_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30029741h1SMcG0006354 Contig48015newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30029741h1SMcG0006354 Contig48015uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30029741h1SMcG0006354 Contig44158newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30029741h1SMcG0006354 Contig44158uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30029741h1SMcG0006354 Contig10030newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30029741h1SMcG0006354 Contig10030uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
neoblastSMED30029741h1SMcG0006354 dd_Smed_v6_2250_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
zeta neoblastSMED30029741h1SMcG0006354 dd_Smed_v4_2250_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
neoblastSMED30029741h1SMcG0006354 SMED_04478_V2_1GPL14150PMID:22385657
Wagner et al., 2012		whole organism asexual adult cDNA to DNA expression microarray evidence
neoblastSMED30029741h1SMcG0006354 SMED_04478_V2_1GPL14150_gene_modelsPMID:22385657
Wagner et al., 2012		whole organism asexual adult cDNA to DNA expression microarray evidence
Note: Hover over icons to view figure legend
Alignments
SMED30029741 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.002837:58744..62774 -6990
Homology
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BLAST of SMED30029741 vs. Planmine SMEST
Match: SMESG000056346.1 (SMESG000056346.1)

HSP 1 Score: 691.419 bits (1783), Expect = 0.000e+0
Identity = 424/426 (99.53%), Postives = 425/426 (99.77%), Query Frame = 1 
Query:   49 METTNVDLENKTNTTSATEDSLNSAKSDKEEMATPKSQAENQLQPSNEVNSHSNEPTDNGDTIVEQPEINSKHIQIILEKSVKAVHDIKTIISEFEKSISKLTKLVGVNCKHSIQEIEMKDFLKNFDNLRKFQANGSHNELKQRINSQKPARYESSRKNEDTPNTRKSKPVKNLDKRQITYRSESPTESDEEEQSNESIYDPSEQSTKYRGDTSRNRSSTRRAVXXXXXXXXXXXXQQNNDESSNSPVETRERKGRGSPRKYVPEDELSSASNTEVVPKKSRGRPKKAVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXIVEDQSSTSIKDPKXXXXXXXXXXXXXXXXXXXXXXXXXQEAKVVSEKSKENVEKRHKGRPRKIVNDEKTPQGKSKRDSNNTNVENSIVRSRGRPKKIKNDAPVEST 1326
            METTNVDLENKTNTTSATEDSLNSAKSDKEEMATPKSQAENQLQPSNEVNSHSNEPTDNGDTIVEQPEINSKHIQIILEKSVKAVHDIKTIISEFEKSISKLTKLVGVNCKHSIQEIEMKDFLKNFDNLRKFQANGSHNELKQRINSQKPARYESSRKNEDTPNTRKSKPVKNLDKRQITYRSESPTESDEEEQSNESIYDPSEQSTKYRGDTSRNRSSTRRAVKSGRKSRGRPRKQQNNDESSNSPVETRERKGRGSPRKYVPEDELSSASNTEVVPKKSRGRPKKAVNNNEESSESEPPAKKSRGRPKKSRGRPNK +VEDQSSTSIKDPKDNHSNNQQESESEESKDKESTNSNEQEAKVVSEKSKENVEKRHKGRPRKIVNDEKTPQGKSKRDSNNTNVENSIVRSRGRPKKIKNDAPVEST
Sbjct:    1 METTNVDLENKTNTTSATEDSLNSAKSDKEEMATPKSQAENQLQPSNEVNSHSNEPTDNGDTIVEQPEINSKHIQIILEKSVKAVHDIKTIISEFEKSISKLTKLVGVNCKHSIQEIEMKDFLKNFDNLRKFQANGSHNELKQRINSQKPARYESSRKNEDTPNTRKSKPVKNLDKRQITYRSESPTESDEEEQSNESIYDPSEQSTKYRGDTSRNRSSTRRAVKSGRKSRGRPRKQQNNDESSNSPVETRERKGRGSPRKYVPEDELSSASNTEVVPKKSRGRPKKAVNNNEESSESEPPAKKSRGRPKKSRGRPNKTVVEDQSSTSIKDPKDNHSNNQQESESEESKDKESTNSNEQEAKVVSEKSKENVEKRHKGRPRKIVNDEKTPQGKSKRDSNNTNVENSIVRSRGRPKKIKNDAPVEST 426
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BLAST of SMED30029741 vs. Planmine SMEST
Match: SMESG000056346.1 (SMESG000056346.1)

HSP 1 Score: 687.182 bits (1772), Expect = 0.000e+0
Identity = 424/427 (99.30%), Postives = 425/427 (99.53%), Query Frame = 1 
Query:   49 METTNVDLENKTNTTSATEDSLNS-AKSDKEEMATPKSQAENQLQPSNEVNSHSNEPTDNGDTIVEQPEINSKHIQIILEKSVKAVHDIKTIISEFEKSISKLTKLVGVNCKHSIQEIEMKDFLKNFDNLRKFQANGSHNELKQRINSQKPARYESSRKNEDTPNTRKSKPVKNLDKRQITYRSESPTESDEEEQSNESIYDPSEQSTKYRGDTSRNRSSTRRAVXXXXXXXXXXXXQQNNDESSNSPVETRERKGRGSPRKYVPEDELSSASNTEVVPKKSRGRPKKAVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXIVEDQSSTSIKDPKXXXXXXXXXXXXXXXXXXXXXXXXXQEAKVVSEKSKENVEKRHKGRPRKIVNDEKTPQGKSKRDSNNTNVENSIVRSRGRPKKIKNDAPVEST 1326
            METTNVDLENKTNTTSATEDSLNS AKSDKEEMATPKSQAENQLQPSNEVNSHSNEPTDNGDTIVEQPEINSKHIQIILEKSVKAVHDIKTIISEFEKSISKLTKLVGVNCKHSIQEIEMKDFLKNFDNLRKFQANGSHNELKQRINSQKPARYESSRKNEDTPNTRKSKPVKNLDKRQITYRSESPTESDEEEQSNESIYDPSEQSTKYRGDTSRNRSSTRRAVKSGRKSRGRPRKQQNNDESSNSPVETRERKGRGSPRKYVPEDELSSASNTEVVPKKSRGRPKKAVNNNEESSESEPPAKKSRGRPKKSRGRPNK +VEDQSSTSIKDPKDNHSNNQQESESEESKDKESTNSNEQEAKVVSEKSKENVEKRHKGRPRKIVNDEKTPQGKSKRDSNNTNVENSIVRSRGRPKKIKNDAPVEST
Sbjct:    1 METTNVDLENKTNTTSATEDSLNSSAKSDKEEMATPKSQAENQLQPSNEVNSHSNEPTDNGDTIVEQPEINSKHIQIILEKSVKAVHDIKTIISEFEKSISKLTKLVGVNCKHSIQEIEMKDFLKNFDNLRKFQANGSHNELKQRINSQKPARYESSRKNEDTPNTRKSKPVKNLDKRQITYRSESPTESDEEEQSNESIYDPSEQSTKYRGDTSRNRSSTRRAVKSGRKSRGRPRKQQNNDESSNSPVETRERKGRGSPRKYVPEDELSSASNTEVVPKKSRGRPKKAVNNNEESSESEPPAKKSRGRPKKSRGRPNKTVVEDQSSTSIKDPKDNHSNNQQESESEESKDKESTNSNEQEAKVVSEKSKENVEKRHKGRPRKIVNDEKTPQGKSKRDSNNTNVENSIVRSRGRPKKIKNDAPVEST 427
[Close]
BLAST of SMED30029741 vs. Planmine SMEST
Match: SMESG000056346.1 (SMESG000056346.1)

HSP 1 Score: 462.225 bits (1188), Expect = 7.942e-162
Identity = 338/427 (79.16%), Postives = 348/427 (81.50%), Query Frame = 1 
Query:   49 METTNVDLENKTNTTSATEDSLNS-AKSDKEEMATPKSQAENQLQPSNEVNSHSNEPTDNGDTIVEQPEINSKHIQIILEKSVKAVHDIKTIISEFEKSISKLTKLVGVNCKHSIQEIEMKDFLKNFDNLRKFQANGSHNELKQRINSQKPARYESSRKNEDTPNTRKSKPVKNLDKRQITYRSESPTESDEEEQSNESIYDPSEQSTKYRGDTSRNRSSTRRAVXXXXXXXXXXXXQQNNDESSNSPVETRERKGRGSPRKYVPEDELSSASNTEVVPKKSRGRPKKAVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXIVEDQSSTSIKDPKXXXXXXXXXXXXXXXXXXXXXXXXXQEAKVVSEKSKENVEKRHKGRPRKIVNDEKTPQGKSKRDSNNTNVENSIVRSRGRPKKIKNDAPVEST 1326
            METTNVDLENKTNTTSATEDSLNS AKSDKEEMATPKSQAENQLQPSNEVNSHSNEPTDNGDT             I+ +  + + H I+ I+   EKS+  +         H I                                          +KNEDTPNTRKSKPVKNLDKRQITYRSESPTESDEEEQSNESIYDPSEQSTKYRGDTSRNRSSTRRAVKSGRKSRGRPRKQQNNDESSNSPVETRERKGRGSPRKYVPEDELSSASNTEVVPKKSRGRPKKAVNNNEESSESEPPAKKSRGRPKKSRGRPNK +VEDQSSTSIKDPKDNHSNNQQESESEESKDKESTNSNEQEAKVVSEKSKENVEKRHKGRPRKIVNDEKTPQGKSKRDSNNTNVENSIVRSRGRPKKIKNDAPVEST
Sbjct:    1 METTNVDLENKTNTTSATEDSLNSSAKSDKEEMATPKSQAENQLQPSNEVNSHSNEPTDNGDT-------------IVEQPEINSKH-IQIIL---EKSVKAV---------HDI------------------------------------------KKNEDTPNTRKSKPVKNLDKRQITYRSESPTESDEEEQSNESIYDPSEQSTKYRGDTSRNRSSTRRAVKSGRKSRGRPRKQQNNDESSNSPVETRERKGRGSPRKYVPEDELSSASNTEVVPKKSRGRPKKAVNNNEESSESEPPAKKSRGRPKKSRGRPNKTVVEDQSSTSIKDPKDNHSNNQQESESEESKDKESTNSNEQEAKVVSEKSKENVEKRHKGRPRKIVNDEKTPQGKSKRDSNNTNVENSIVRSRGRPKKIKNDAPVEST 359
The following BLAST results are available for this feature:
BLAST of SMED30029741 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30029741 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30029741 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30029741 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30029741 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30029741 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30029741 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30029741 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30029741 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30029741 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30029741 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30029741 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30029741 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30029741 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3
Match NameE-valueIdentityDescription
SMESG000056346.10.000e+099.53SMESG000056346.1[more]
SMESG000056346.10.000e+099.30SMESG000056346.1[more]
SMESG000056346.17.942e-16279.16SMESG000056346.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30029741 ID=SMED30029741|Name=SMED30029741|organism=Schmidtea mediterranea sexual|type=transcript|length=1439bp
ATCTTTTATTTGTTTAAATAAACATACAATACATTTGAATATTTTTAAAT
GGAGACTACCAATGTCGATCTTGAAAATAAAACAAACACAACATCAGCAA
CTGAAGATTCTTTAAATTCTGCGAAGTCAGACAAGGAGGAAATGGCAACA
CCAAAGAGTCAAGCTGAAAATCAATTACAACCTTCTAATGAAGTCAATTC
TCATTCAAACGAACCAACTGATAATGGCGATACGATAGTAGAACAGCCAG
AAATTAATAGCAAACACATTCAAATAATATTAGAAAAAAGCGTCAAAGCA
GTTCATGATATTAAAACCATTATCTCAGAATTCGAAAAGAGTATTTCGAA
GCTTACGAAATTAGTTGGTGTAAATTGTAAACATAGTATACAAGAAATAG
AAATGAAGGATTTTCTAAAAAATTTTGATAATTTAAGAAAATTTCAAGCA
AATGGTTCACACAATGAGCTAAAACAAAGAATTAATTCTCAAAAACCTGC
ACGATATGAAAGTTCCAGAAAAAATGAGGATACCCCAAACACAAGAAAAT
CCAAGCCAGTTAAAAATTTAGATAAACGACAAATTACGTATCGGAGTGAG
TCTCCAACGGAAAGCGATGAAGAAGAACAATCCAATGAATCTATCTATGA
TCCTTCAGAACAAAGCACAAAATATAGAGGCGATACAAGTCGCAATCGCA
GTTCCACAAGAAGAGCAGTTAAAAGCGGAAGAAAGTCACGAGGACGTCCA
CGTAAACAGCAAAACAATGATGAAAGTTCCAATTCTCCTGTTGAAACAAG
AGAGAGAAAAGGTCGTGGCTCGCCACGCAAATATGTACCAGAAGATGAAC
TAAGTTCTGCTTCAAATACTGAAGTTGTTCCAAAAAAATCCCGAGGAAGA
CCTAAAAAAGCAGTGAATAATAACGAAGAGTCATCAGAATCGGAACCACC
AGCTAAAAAATCACGTGGTAGACCGAAAAAGTCACGAGGAAGACCCAATA
AGCCTATTGTTGAAGATCAATCATCAACTTCTATAAAAGATCCCAAGGAC
AATCATTCAAATAATCAACAAGAATCGGAATCAGAGGAATCTAAAGATAA
AGAATCGACGAATAGCAATGAACAGGAAGCAAAGGTTGTATCAGAAAAAT
CAAAAGAGAACGTGGAAAAAAGACATAAAGGTCGTCCTAGGAAAATTGTA
AATGATGAAAAAACTCCCCAAGGAAAATCTAAAAGGGATTCAAATAATAC
TAATGTAGAAAATTCAATTGTTAGGTCTAGGGGAAGACCGAAAAAAATTA
AAAATGATGCTCCTGTCGAATCAACTTAAAAATACGACTCATGTGAATTG
TGTTCAGTTATAACATTTTAAAACGATTTTATTAGAAACTGTTGCATCCA
ATTTTCAATGTATTTATGTGGTCATTTATTATAATAAAT
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protein sequence of SMED30029741-orf-1

>SMED30029741-orf-1 ID=SMED30029741-orf-1|Name=SMED30029741-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=427bp
METTNVDLENKTNTTSATEDSLNSAKSDKEEMATPKSQAENQLQPSNEVN
SHSNEPTDNGDTIVEQPEINSKHIQIILEKSVKAVHDIKTIISEFEKSIS
KLTKLVGVNCKHSIQEIEMKDFLKNFDNLRKFQANGSHNELKQRINSQKP
ARYESSRKNEDTPNTRKSKPVKNLDKRQITYRSESPTESDEEEQSNESIY
DPSEQSTKYRGDTSRNRSSTRRAVKSGRKSRGRPRKQQNNDESSNSPVET
RERKGRGSPRKYVPEDELSSASNTEVVPKKSRGRPKKAVNNNEESSESEP
PAKKSRGRPKKSRGRPNKPIVEDQSSTSIKDPKDNHSNNQQESESEESKD
KESTNSNEQEAKVVSEKSKENVEKRHKGRPRKIVNDEKTPQGKSKRDSNN
TNVENSIVRSRGRPKKIKNDAPVEST*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000014zeta neoblast
PLANA:0000429neoblast
PLANA:0002109X1 cell
PLANA:0002111X2 cell
Vocabulary: INTERPRO
TermDefinition
IPR017956AT_hook_DNA-bd_motif
Vocabulary: molecular function
TermDefinition
GO:0003677DNA binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR017956AT hook, DNA-binding motifPRINTSPR00929ATHOOKcoord: 228..238
score: 55.3
coord: 277..288
score: 54.86
coord: 301..311
score: 60.23
IPR017956AT hook, DNA-binding motifSMARTSM00384AT_hook_2coord: 303..315
e-value: 31.0
score: 6.9
coord: 374..386
e-value: 21.0
score: 7.9
coord: 228..240
e-value: 8.0
score: 10.2
coord: 279..291
e-value: 2.1
score: 13.4
coord: 253..265
e-value: 120.0
score: 3.5
coord: 408..420
e-value: 1.1
score: 15.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 327..394
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..22
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 246..263
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 409..426
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 36..57
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 135..426
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..57
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 145..189
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 279..305