U6 snRNA-associated Sm-like protein LSm4

Overview
NameU6 snRNA-associated Sm-like protein LSm4
Smed IDSMED30029609
Length (bp)508
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of U6 snRNA-associated Sm-like protein LSm4 (SMED30029609) t-SNE clustered cells

Violin plots show distribution of expression levels for U6 snRNA-associated Sm-like protein LSm4 (SMED30029609) in cells (dots) of each of the 12 neoblast clusters.

 

back to top

 

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of U6 snRNA-associated Sm-like protein LSm4 (SMED30029609) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for U6 snRNA-associated Sm-like protein LSm4 (SMED30029609) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

back to top

 

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

back to top
Anatomical Expression

PAGE et. al., 2020



SMED30029609

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30029609SMESG000053212.1 SmedASXL_005221SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30029609SMESG000053212.1 SmedASXL_005221SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of U6 snRNA-associated Sm-like protein LSm4 vs. Ensembl Human
Match: LSM4 (LSM4 homolog, U6 small nuclear RNA and mRNA degradation associated [Source:NCBI gene;Acc:25804])

HSP 1 Score: 122.865 bits (307), Expect = 7.452e-36
Identity = 55/87 (63.22%), Postives = 69/87 (79.31%), Query Frame = 2 
Query:   41 MLPLSLIKACINKPILVELKNNETYNGHLAIVDSFMNIQLKKAICTSKDGDKFWSIPEVYIKGNTIKFIRIPPDVIDNVNEEVKLKA 301
            MLPLSL+K   N P+LVELKN ETYNGHL   D++MNI L++ ICTS+DGDKFW +PE YI+G+TIK++RIP ++ID V EEV  K 
Sbjct:    1 MLPLSLLKTAQNHPMLVELKNGETYNGHLVSCDNWMNINLREVICTSRDGDKFWRMPECYIRGSTIKYLRIPDEIIDMVKEEVVAKG 87
[Close]
BLAST of U6 snRNA-associated Sm-like protein LSm4 vs. Ensembl Human
Match: LSM4 (LSM4 homolog, U6 small nuclear RNA and mRNA degradation associated [Source:NCBI gene;Acc:25804])

HSP 1 Score: 125.176 bits (313), Expect = 1.605e-35
Identity = 55/87 (63.22%), Postives = 69/87 (79.31%), Query Frame = 2 
Query:   41 MLPLSLIKACINKPILVELKNNETYNGHLAIVDSFMNIQLKKAICTSKDGDKFWSIPEVYIKGNTIKFIRIPPDVIDNVNEEVKLKA 301
            MLPLSL+K   N P+LVELKN ETYNGHL   D++MNI L++ ICTS+DGDKFW +PE YI+G+TIK++RIP ++ID V EEV  K 
Sbjct:    1 MLPLSLLKTAQNHPMLVELKNGETYNGHLVSCDNWMNINLREVICTSRDGDKFWRMPECYIRGSTIKYLRIPDEIIDMVKEEVVAKG 87
[Close]
BLAST of U6 snRNA-associated Sm-like protein LSm4 vs. Ensembl Human
Match: LSM4 (LSM4 homolog, U6 small nuclear RNA and mRNA degradation associated [Source:NCBI gene;Acc:25804])

HSP 1 Score: 105.916 bits (263), Expect = 2.116e-29
Identity = 46/73 (63.01%), Postives = 59/73 (80.82%), Query Frame = 2 
Query:   83 ILVELKNNETYNGHLAIVDSFMNIQLKKAICTSKDGDKFWSIPEVYIKGNTIKFIRIPPDVIDNVNEEVKLKA 301
            +LVELKN ETYNGHL   D++MNI L++ ICTS+DGDKFW +PE YI+G+TIK++RIP ++ID V EEV  K 
Sbjct:    1 MLVELKNGETYNGHLVSCDNWMNINLREVICTSRDGDKFWRMPECYIRGSTIKYLRIPDEIIDMVKEEVVAKG 73
[Close]
BLAST of U6 snRNA-associated Sm-like protein LSm4 vs. Ensembl Human
Match: LSM4 (LSM4 homolog, U6 small nuclear RNA and mRNA degradation associated [Source:NCBI gene;Acc:25804])

HSP 1 Score: 69.3218 bits (168), Expect = 9.883e-16
Identity = 33/63 (52.38%), Postives = 43/63 (68.25%), Query Frame = 2 
Query:   41 MLPLSLIKACINKPILVELKNNETYNGHLAIVDSFMNIQLKKAICTSKDGDKFWSIPEVYIKG 229
            MLPLSL+K   N P+LVELKN ETYNGHL   D++MNI L++ ICTS+  +     P   ++G
Sbjct:    1 MLPLSLLKTAQNHPMLVELKNGETYNGHLVSCDNWMNINLREVICTSRVKNPNQREPHPRLEG 63
[Close]
BLAST of U6 snRNA-associated Sm-like protein LSm4 vs. Ensembl Human
Match: LSM2 (LSM2 homolog, U6 small nuclear RNA and mRNA degradation associated [Source:HGNC Symbol;Acc:HGNC:13940])

HSP 1 Score: 49.6766 bits (117), Expect = 4.856e-8
Identity = 26/81 (32.10%), Postives = 48/81 (59.26%), Query Frame = 2 
Query:   41 MLPLSLIKACINKPILVELKNNETYNGHLAIVDSFMNIQLKKAICTSKDGDKF---WSIPEVYIKGNTIKFIRIPPDVIDN 274
            ML  S  K+ + K ++VELKN+ +  G L  VD ++NI+L     T  D +K+    S+   +I+G+ ++++++P D +D 
Sbjct:    1 MLFYSFFKSLVGKDVVVELKNDLSICGTLHSVDQYLNIKLTDISVT--DPEKYPHMLSVKNCFIRGSVVRYVQLPADEVDT 79
[Close]
BLAST of U6 snRNA-associated Sm-like protein LSm4 vs. Ensembl Celegans
Match: lsm-4 (Probable U6 snRNA-associated Sm-like protein LSm4 [Source:UniProtKB/Swiss-Prot;Acc:Q19952])

HSP 1 Score: 106.686 bits (265), Expect = 4.731e-30
Identity = 50/83 (60.24%), Postives = 62/83 (74.70%), Query Frame = 2 
Query:   41 MLPLSLIKACINKPILVELKNNETYNGHLAIVDSFMNIQLKKAICTSKDGDKFWSIPEVYIKGNTIKFIRIPPDVIDNVNEEV 289
            +LPLSL+K   N P+LVELKN ETYNGHL   DS+MNI L   I TSKDGDKF+ + E Y++G+TIK++RIP  V+D V  EV
Sbjct:    2 VLPLSLLKTAQNHPMLVELKNGETYNGHLKACDSWMNIHLVDVIFTSKDGDKFFKMSEAYVRGSTIKYLRIPETVVDLVKTEV 84
[Close]
BLAST of U6 snRNA-associated Sm-like protein LSm4 vs. Ensembl Celegans
Match: lsm-4 (Probable U6 snRNA-associated Sm-like protein LSm4 [Source:UniProtKB/Swiss-Prot;Acc:Q19952])

HSP 1 Score: 106.686 bits (265), Expect = 4.731e-30
Identity = 50/83 (60.24%), Postives = 62/83 (74.70%), Query Frame = 2 
Query:   41 MLPLSLIKACINKPILVELKNNETYNGHLAIVDSFMNIQLKKAICTSKDGDKFWSIPEVYIKGNTIKFIRIPPDVIDNVNEEV 289
            +LPLSL+K   N P+LVELKN ETYNGHL   DS+MNI L   I TSKDGDKF+ + E Y++G+TIK++RIP  V+D V  EV
Sbjct:    2 VLPLSLLKTAQNHPMLVELKNGETYNGHLKACDSWMNIHLVDVIFTSKDGDKFFKMSEAYVRGSTIKYLRIPETVVDLVKTEV 84
[Close]
BLAST of U6 snRNA-associated Sm-like protein LSm4 vs. Ensembl Celegans
Match: gut-2 (U6 snRNA-associated Sm-like protein LSm2 [Source:UniProtKB/TrEMBL;Acc:E1B6V1])

HSP 1 Score: 50.0618 bits (118), Expect = 1.555e-8
Identity = 25/81 (30.86%), Postives = 48/81 (59.26%), Query Frame = 2 
Query:   41 MLPLSLIKACINKPILVELKNNETYNGHLAIVDSFMNIQLKKAICTSKDGDKF---WSIPEVYIKGNTIKFIRIPPDVIDN 274
            ML  S  K+ + K ++VELKN+ +  G L  VD ++N++L     T  D ++F    S+   +I+G+ ++++++P D +D 
Sbjct:    1 MLFFSFFKSLVGKDVVVELKNDLSICGTLHSVDQYLNMKLTDI--TVSDPERFPHMVSVKNCFIRGSVVRYVQLPSDQVDT 79
[Close]
BLAST of U6 snRNA-associated Sm-like protein LSm4 vs. Ensembl Celegans
Match: snr-1 (Small nuclear ribonucleoprotein Sm D3 [Source:UniProtKB/Swiss-Prot;Acc:Q17348])

HSP 1 Score: 45.4394 bits (106), Expect = 1.629e-6
Identity = 23/78 (29.49%), Postives = 42/78 (53.85%), Query Frame = 2 
Query:   44 LPLSLIKACINKPILVELKNNETYNGHLAIVDSFMNIQLKKAICTSKDGDKFWSIPEVYIKGNTIKFIRIPPDVIDNV 277
            +P+ ++       + +E    E Y G L+  +  MN QL + + T +DG +   +  V+I+GN I+F+ I PD++ N 
Sbjct:    6 VPIKILHEAEGHMVTLETVTGEVYRGKLSEAEDNMNCQLAETVVTFRDG-RSHQLDNVFIRGNKIRFM-ILPDMLKNA 81
[Close]
BLAST of U6 snRNA-associated Sm-like protein LSm4 vs. Ensembl Fly
Match: CG17768 (gene:FBgn0032240 transcript:FBtr0100253)

HSP 1 Score: 125.946 bits (315), Expect = 3.037e-37
Identity = 56/91 (61.54%), Postives = 71/91 (78.02%), Query Frame = 2 
Query:   41 MLPLSLIKACINKPILVELKNNETYNGHLAIVDSFMNIQLKKAICTSKDGDKFWSIPEVYIKGNTIKFIRIPPDVIDNVNEEVKLKAPPRT 313
            MLPLSL+K   + P+LVELKN ETYNGHL   DS+MNI L+  ICTSKDGD+FW +PE YI+G+TIK++RIP +VID V E+ + K+  RT
Sbjct:    1 MLPLSLLKTAQSHPMLVELKNGETYNGHLVSCDSWMNINLRDVICTSKDGDRFWRMPECYIRGSTIKYLRIPDEVIDMVKEDAQAKSRNRT 91
[Close]
BLAST of U6 snRNA-associated Sm-like protein LSm4 vs. Ensembl Fly
Match: CG17768 (gene:FBgn0032240 transcript:FBtr0304111)

HSP 1 Score: 125.946 bits (315), Expect = 3.037e-37
Identity = 56/91 (61.54%), Postives = 71/91 (78.02%), Query Frame = 2 
Query:   41 MLPLSLIKACINKPILVELKNNETYNGHLAIVDSFMNIQLKKAICTSKDGDKFWSIPEVYIKGNTIKFIRIPPDVIDNVNEEVKLKAPPRT 313
            MLPLSL+K   + P+LVELKN ETYNGHL   DS+MNI L+  ICTSKDGD+FW +PE YI+G+TIK++RIP +VID V E+ + K+  RT
Sbjct:    1 MLPLSLLKTAQSHPMLVELKNGETYNGHLVSCDSWMNINLRDVICTSKDGDRFWRMPECYIRGSTIKYLRIPDEVIDMVKEDAQAKSRNRT 91
[Close]
BLAST of U6 snRNA-associated Sm-like protein LSm4 vs. Ensembl Fly
Match: CG10418 (gene:FBgn0036277 transcript:FBtr0075954)

HSP 1 Score: 51.2174 bits (121), Expect = 6.438e-9
Identity = 27/81 (33.33%), Postives = 48/81 (59.26%), Query Frame = 2 
Query:   41 MLPLSLIKACINKPILVELKNNETYNGHLAIVDSFMNIQLKKAICTSKDGDKF---WSIPEVYIKGNTIKFIRIPPDVIDN 274
            ML  S  K+ + K ++VELKN+ +  G L  VD ++NI+L     T  D DK+    S+   +I+G+ ++++++P D +D 
Sbjct:    1 MLFYSFFKSLVGKEVVVELKNDLSICGTLHSVDQYLNIKLTDISVT--DPDKYPHMLSVKNCFIRGSVVRYVQLPGDEVDT 79
[Close]
BLAST of U6 snRNA-associated Sm-like protein LSm4 vs. Ensembl Zebrafish
Match: lsm4 (LSM4 homolog, U6 small nuclear RNA and mRNA degradation associated [Source:ZFIN;Acc:ZDB-GENE-040426-1652])

HSP 1 Score: 124.79 bits (312), Expect = 9.665e-37
Identity = 56/90 (62.22%), Postives = 70/90 (77.78%), Query Frame = 2 
Query:   41 MLPLSLIKACINKPILVELKNNETYNGHLAIVDSFMNIQLKKAICTSKDGDKFWSIPEVYIKGNTIKFIRIPPDVIDNVNEEVKLKAPPR 310
            MLPLSL+K   N P+LVELKN ETYNGHL   D++MNI L++ ICTS+DGDKFW +PE YI+G+TIK++RIP ++ID V EEV  K   R
Sbjct:    1 MLPLSLLKTAQNHPMLVELKNGETYNGHLVSCDNWMNINLREVICTSRDGDKFWRMPECYIRGSTIKYLRIPDEIIDMVKEEVVSKGRGR 90
[Close]
BLAST of U6 snRNA-associated Sm-like protein LSm4 vs. Ensembl Zebrafish
Match: lsm4 (LSM4 homolog, U6 small nuclear RNA and mRNA degradation associated [Source:ZFIN;Acc:ZDB-GENE-040426-1652])

HSP 1 Score: 107.842 bits (268), Expect = 2.806e-30
Identity = 47/76 (61.84%), Postives = 60/76 (78.95%), Query Frame = 2 
Query:   83 ILVELKNNETYNGHLAIVDSFMNIQLKKAICTSKDGDKFWSIPEVYIKGNTIKFIRIPPDVIDNVNEEVKLKAPPR 310
            +LVELKN ETYNGHL   D++MNI L++ ICTS+DGDKFW +PE YI+G+TIK++RIP ++ID V EEV  K   R
Sbjct:    1 MLVELKNGETYNGHLVSCDNWMNINLREVICTSRDGDKFWRMPECYIRGSTIKYLRIPDEIIDMVKEEVVSKGRGR 76
[Close]
BLAST of U6 snRNA-associated Sm-like protein LSm4 vs. Ensembl Zebrafish
Match: smx5 (smx5 [Source:ZFIN;Acc:ZDB-GENE-000616-10])

HSP 1 Score: 50.0618 bits (118), Expect = 2.754e-8
Identity = 26/81 (32.10%), Postives = 48/81 (59.26%), Query Frame = 2 
Query:   41 MLPLSLIKACINKPILVELKNNETYNGHLAIVDSFMNIQLKKAICTSKDGDKF---WSIPEVYIKGNTIKFIRIPPDVIDN 274
            ML  S  K+ + K ++VELKN+ +  G L  VD ++NI+L     T  D +K+    S+   +I+G+ ++++++P D +D 
Sbjct:    1 MLFYSFFKSLVGKDVVVELKNDLSICGTLHSVDQYLNIKLTDISVT--DPEKYPHMLSVKNCFIRGSVVRYVQLPADEVDT 79
[Close]
BLAST of U6 snRNA-associated Sm-like protein LSm4 vs. Ensembl Zebrafish
Match: LSM2 (si:ch73-14h10.2 [Source:ZFIN;Acc:ZDB-GENE-141222-67])

HSP 1 Score: 50.0618 bits (118), Expect = 2.754e-8
Identity = 26/81 (32.10%), Postives = 48/81 (59.26%), Query Frame = 2 
Query:   41 MLPLSLIKACINKPILVELKNNETYNGHLAIVDSFMNIQLKKAICTSKDGDKF---WSIPEVYIKGNTIKFIRIPPDVIDN 274
            ML  S  K+ + K ++VELKN+ +  G L  VD ++NI+L     T  D +K+    S+   +I+G+ ++++++P D +D 
Sbjct:    1 MLFYSFFKSLVGKDVVVELKNDLSICGTLHSVDQYLNIKLTDISVT--DPEKYPHMLSVKNCFIRGSVVRYVQLPADEVDT 79
[Close]
BLAST of U6 snRNA-associated Sm-like protein LSm4 vs. Ensembl Zebrafish
Match: smx5 (smx5 [Source:ZFIN;Acc:ZDB-GENE-000616-10])

HSP 1 Score: 47.7506 bits (112), Expect = 2.100e-7
Identity = 25/80 (31.25%), Postives = 47/80 (58.75%), Query Frame = 2 
Query:   44 LPLSLIKACINKPILVELKNNETYNGHLAIVDSFMNIQLKKAICTSKDGDKF---WSIPEVYIKGNTIKFIRIPPDVIDN 274
            L  S  K+ + K ++VELKN+ +  G L  VD ++NI+L     T  D +K+    S+   +I+G+ ++++++P D +D 
Sbjct:    7 LFYSFFKSLVGKDVVVELKNDLSICGTLHSVDQYLNIKLTDISVT--DPEKYPHMLSVKNCFIRGSVVRYVQLPADEVDT 84
[Close]
BLAST of U6 snRNA-associated Sm-like protein LSm4 vs. Ensembl Xenopus
Match: nenf (Xenopus tropicalis LSM4 homolog, U6 small nuclear RNA and mRNA degradation associated (lsm4), mRNA. [Source:RefSeq mRNA;Acc:NM_204040])

HSP 1 Score: 125.176 bits (313), Expect = 5.943e-35
Identity = 56/90 (62.22%), Postives = 70/90 (77.78%), Query Frame = 2 
Query:   41 MLPLSLIKACINKPILVELKNNETYNGHLAIVDSFMNIQLKKAICTSKDGDKFWSIPEVYIKGNTIKFIRIPPDVIDNVNEEVKLKAPPR 310
            MLPLSL+K   N P+LVELKN ETYNGHL   D++MNI L++ ICTS+DGDKFW +PE YI+G+TIK++RIP ++ID V EEV  K   R
Sbjct:    1 MLPLSLLKTAQNHPMLVELKNGETYNGHLVSCDNWMNINLREVICTSRDGDKFWRMPECYIRGSTIKYLRIPDEIIDMVKEEVMSKGRGR 90
[Close]
BLAST of U6 snRNA-associated Sm-like protein LSm4 vs. Ensembl Xenopus
Match: nenf (Xenopus tropicalis LSM4 homolog, U6 small nuclear RNA and mRNA degradation associated (lsm4), mRNA. [Source:RefSeq mRNA;Acc:NM_204040])

HSP 1 Score: 108.227 bits (269), Expect = 3.049e-30
Identity = 47/75 (62.67%), Postives = 59/75 (78.67%), Query Frame = 2 
Query:   86 LVELKNNETYNGHLAIVDSFMNIQLKKAICTSKDGDKFWSIPEVYIKGNTIKFIRIPPDVIDNVNEEVKLKAPPR 310
            LVELKN ETYNGHL   D++MNI L++ ICTS+DGDKFW +PE YI+G+TIK++RIP ++ID V EEV  K   R
Sbjct:    7 LVELKNGETYNGHLVSCDNWMNINLREVICTSRDGDKFWRMPECYIRGSTIKYLRIPDEIIDMVKEEVMSKGRGR 81
[Close]
BLAST of U6 snRNA-associated Sm-like protein LSm4 vs. Ensembl Xenopus
Match: nipa2 (NIPA magnesium transporter 2 [Source:Xenbase;Acc:XB-GENE-973321])

HSP 1 Score: 50.0618 bits (118), Expect = 4.178e-8
Identity = 26/81 (32.10%), Postives = 48/81 (59.26%), Query Frame = 2 
Query:   41 MLPLSLIKACINKPILVELKNNETYNGHLAIVDSFMNIQLKKAICTSKDGDKF---WSIPEVYIKGNTIKFIRIPPDVIDN 274
            ML  S  K+ + K ++VELKN+ +  G L  VD ++NI+L     T  D +K+    S+   +I+G+ ++++++P D +D 
Sbjct:    1 MLFYSFFKSLVGKDVVVELKNDLSICGTLHSVDQYLNIKLTDISVT--DPEKYPHMLSVKNCFIRGSVVRYVQLPADEVDT 79
[Close]
BLAST of U6 snRNA-associated Sm-like protein LSm4 vs. Ensembl Mouse
Match: Lsm4 (LSM4 homolog, U6 small nuclear RNA and mRNA degradation associated [Source:MGI Symbol;Acc:MGI:1354692])

HSP 1 Score: 121.709 bits (304), Expect = 1.540e-35
Identity = 53/83 (63.86%), Postives = 67/83 (80.72%), Query Frame = 2 
Query:   41 MLPLSLIKACINKPILVELKNNETYNGHLAIVDSFMNIQLKKAICTSKDGDKFWSIPEVYIKGNTIKFIRIPPDVIDNVNEEV 289
            MLPLSL+K   N P+LVELKN ETYNGHL   D++MNI L++ ICTS+DGDKFW +PE YI+G+TIK++RIP ++ID V EE 
Sbjct:    1 MLPLSLLKTAQNHPMLVELKNGETYNGHLVSCDNWMNINLREVICTSRDGDKFWRMPECYIRGSTIKYLRIPDEIIDMVREEA 83
[Close]
BLAST of U6 snRNA-associated Sm-like protein LSm4 vs. Ensembl Mouse
Match: Lsm4 (LSM4 homolog, U6 small nuclear RNA and mRNA degradation associated [Source:MGI Symbol;Acc:MGI:1354692])

HSP 1 Score: 121.709 bits (304), Expect = 1.540e-35
Identity = 53/83 (63.86%), Postives = 67/83 (80.72%), Query Frame = 2 
Query:   41 MLPLSLIKACINKPILVELKNNETYNGHLAIVDSFMNIQLKKAICTSKDGDKFWSIPEVYIKGNTIKFIRIPPDVIDNVNEEV 289
            MLPLSL+K   N P+LVELKN ETYNGHL   D++MNI L++ ICTS+DGDKFW +PE YI+G+TIK++RIP ++ID V EE 
Sbjct:    1 MLPLSLLKTAQNHPMLVELKNGETYNGHLVSCDNWMNINLREVICTSRDGDKFWRMPECYIRGSTIKYLRIPDEIIDMVREEA 83
[Close]
BLAST of U6 snRNA-associated Sm-like protein LSm4 vs. Ensembl Mouse
Match: Lsm4 (LSM4 homolog, U6 small nuclear RNA and mRNA degradation associated [Source:MGI Symbol;Acc:MGI:1354692])

HSP 1 Score: 104.76 bits (260), Expect = 1.700e-29
Identity = 49/83 (59.04%), Postives = 62/83 (74.70%), Query Frame = 2 
Query:   41 MLPLSLIKACINKPILVELKNNETYNGHLAIVDSFMNIQLKKAICTSKDGDKFWSIPEVYIKGNTIKFIRIPPDVIDNVNEEV 289
            MLPLSL+K   N P+LVELKN ETYNGHL   D++MNI L+      +DGDKFW +PE YI+G+TIK++RIP ++ID V EE 
Sbjct:    1 MLPLSLLKTAQNHPMLVELKNGETYNGHLVSCDNWMNINLR------EDGDKFWRMPECYIRGSTIKYLRIPDEIIDMVREEA 77
[Close]
BLAST of U6 snRNA-associated Sm-like protein LSm4 vs. Ensembl Mouse
Match: Lsm4 (LSM4 homolog, U6 small nuclear RNA and mRNA degradation associated [Source:MGI Symbol;Acc:MGI:1354692])

HSP 1 Score: 104.76 bits (260), Expect = 1.700e-29
Identity = 49/83 (59.04%), Postives = 62/83 (74.70%), Query Frame = 2 
Query:   41 MLPLSLIKACINKPILVELKNNETYNGHLAIVDSFMNIQLKKAICTSKDGDKFWSIPEVYIKGNTIKFIRIPPDVIDNVNEEV 289
            MLPLSL+K   N P+LVELKN ETYNGHL   D++MNI L+      +DGDKFW +PE YI+G+TIK++RIP ++ID V EE 
Sbjct:    1 MLPLSLLKTAQNHPMLVELKNGETYNGHLVSCDNWMNINLR------EDGDKFWRMPECYIRGSTIKYLRIPDEIIDMVREEA 77
[Close]
BLAST of U6 snRNA-associated Sm-like protein LSm4 vs. Ensembl Mouse
Match: Lsm4 (LSM4 homolog, U6 small nuclear RNA and mRNA degradation associated [Source:MGI Symbol;Acc:MGI:1354692])

HSP 1 Score: 74.7146 bits (182), Expect = 1.279e-17
Identity = 30/48 (62.50%), Postives = 40/48 (83.33%), Query Frame = 2 
Query:  146 MNIQLKKAICTSKDGDKFWSIPEVYIKGNTIKFIRIPPDVIDNVNEEV 289
            MNI L++ ICTS+DGDKFW +PE YI+G+TIK++RIP ++ID V EE 
Sbjct:    1 MNINLREVICTSRDGDKFWRMPECYIRGSTIKYLRIPDEIIDMVREEA 48
[Close]
BLAST of U6 snRNA-associated Sm-like protein LSm4 vs. UniProt/SwissProt
Match: sp|Q43582|LSM4_TOBAC (Probable U6 snRNA-associated Sm-like protein LSm4 OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 125.561 bits (314), Expect = 4.334e-36
Identity = 53/86 (61.63%), Postives = 68/86 (79.07%), Query Frame = 2 
Query:   41 MLPLSLIKACINKPILVELKNNETYNGHLAIVDSFMNIQLKKAICTSKDGDKFWSIPEVYIKGNTIKFIRIPPDVIDNVNEEVKLK 298
            MLPLSL+K     P+LVELKN ETYNGHL   D++MNI L++ ICTSKDGD+FW +PE Y++GNTIK++R+P +VID V EE K +
Sbjct:    1 MLPLSLLKTAQGHPMLVELKNGETYNGHLVNCDTWMNIHLREVICTSKDGDRFWRMPECYVRGNTIKYLRVPDEVIDKVQEEAKSR 86
[Close]
BLAST of U6 snRNA-associated Sm-like protein LSm4 vs. UniProt/SwissProt
Match: sp|Q9ZRU9|LSM4_FAGSY (Probable U6 snRNA-associated Sm-like protein LSm4 OS=Fagus sylvatica OX=28930 GN=LSM4 PE=2 SV=1)

HSP 1 Score: 124.79 bits (312), Expect = 7.829e-36
Identity = 55/92 (59.78%), Postives = 71/92 (77.17%), Query Frame = 2 
Query:   41 MLPLSLIKACINKPILVELKNNETYNGHLAIVDSFMNIQLKKAICTSKDGDKFWSIPEVYIKGNTIKFIRIPPDVIDNVNEEVKLKA---PP 307
            MLPLSL+K     P+LVELK+ ETYNGHL   D++MNI L++ ICTSKDGD+FW +P+ YI+GNTIK++R+P +VID V EE K +A   PP
Sbjct:    1 MLPLSLLKTAQGHPMLVELKSGETYNGHLVNCDTWMNIHLREVICTSKDGDRFWRMPDCYIRGNTIKYLRVPDEVIDKVQEETKSRADRKPP 92
[Close]
BLAST of U6 snRNA-associated Sm-like protein LSm4 vs. UniProt/SwissProt
Match: sp|Q9QXA5|LSM4_MOUSE (U6 snRNA-associated Sm-like protein LSm4 OS=Mus musculus OX=10090 GN=Lsm4 PE=1 SV=1)

HSP 1 Score: 123.635 bits (309), Expect = 2.068e-35
Identity = 57/97 (58.76%), Postives = 71/97 (73.20%), Query Frame = 2 
Query:   41 MLPLSLIKACINKPILVELKNNETYNGHLAIVDSFMNIQLKKAICTSKDGDKFWSIPEVYIKGNTIKFIRIPPDVIDNVNEEVKLKAPPRTTSGSSQ 331
            MLPLSL+K   N P+LVELKN ETYNGHL   D++MNI L++ ICTS+DGDKFW +PE YI+G+TIK++RIP ++ID V EE    A  R   G  Q
Sbjct:    1 MLPLSLLKTAQNHPMLVELKNGETYNGHLVSCDNWMNINLREVICTSRDGDKFWRMPECYIRGSTIKYLRIPDEIIDMVREEA---AKGRGRGGPQQ 94
[Close]
BLAST of U6 snRNA-associated Sm-like protein LSm4 vs. UniProt/SwissProt
Match: sp|Q9LGE6|LSM4_ORYSJ (Probable U6 snRNA-associated Sm-like protein LSm4 OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0256900 PE=2 SV=1)

HSP 1 Score: 123.635 bits (309), Expect = 2.090e-35
Identity = 54/83 (65.06%), Postives = 66/83 (79.52%), Query Frame = 2 
Query:   41 MLPLSLIKACINKPILVELKNNETYNGHLAIVDSFMNIQLKKAICTSKDGDKFWSIPEVYIKGNTIKFIRIPPDVIDNVNEEV 289
            MLPLSL+K     P+LVELKN ETYNGHL   D++MNI L++ ICTSKDGDKFW +PE YI+GNTIK++R+P +VID V EE 
Sbjct:    1 MLPLSLLKTAQGHPMLVELKNGETYNGHLVNCDTWMNIHLREVICTSKDGDKFWRMPECYIRGNTIKYLRVPDEVIDKVQEET 83
[Close]
BLAST of U6 snRNA-associated Sm-like protein LSm4 vs. UniProt/SwissProt
Match: sp|F4K4E3|LSM4_ARATH (Sm-like protein LSM4 OS=Arabidopsis thaliana OX=3702 GN=LSM4 PE=1 SV=1)

HSP 1 Score: 122.865 bits (307), Expect = 3.277e-35
Identity = 53/82 (64.63%), Postives = 66/82 (80.49%), Query Frame = 2 
Query:   41 MLPLSLIKACINKPILVELKNNETYNGHLAIVDSFMNIQLKKAICTSKDGDKFWSIPEVYIKGNTIKFIRIPPDVIDNVNEE 286
            MLPLSL+K     P+LVELKN ETYNGHL   D++MNI L++ ICTSKDGD+FW +PE YI+GNTIK++R+P +VID V EE
Sbjct:    1 MLPLSLLKTAQGHPMLVELKNGETYNGHLVNCDTWMNIHLREVICTSKDGDRFWRMPECYIRGNTIKYLRVPDEVIDKVQEE 82
[Close]
BLAST of U6 snRNA-associated Sm-like protein LSm4 vs. TrEMBL
Match: A0A3Q2VHI0 (U6 snRNA-associated Sm-like protein LSm4 OS=Haplochromis burtoni OX=8153 GN=LSM4 PE=3 SV=1)

HSP 1 Score: 130.954 bits (328), Expect = 2.613e-35
Identity = 61/103 (59.22%), Postives = 77/103 (74.76%), Query Frame = 2 
Query:   23 NFKFIKMLPLSLIKACINKPILVELKNNETYNGHLAIVDSFMNIQLKKAICTSKDGDKFWSIPEVYIKGNTIKFIRIPPDVIDNVNEEVKLKAPPRTTSGSSQ 331
            N + IKMLPLSL+K   N P+LVELKN ETYNGHL   D++MNI L++ ICTS+DGDKFW +PE YI+G+TIK++RIP ++ID V EEV  K   R   G+ Q
Sbjct:   32 NQETIKMLPLSLLKTAQNHPMLVELKNGETYNGHLVSCDNWMNINLREVICTSRDGDKFWRMPECYIRGSTIKYLRIPDEIIDMVKEEVVSKG--RGRGGAQQ 132
[Close]
BLAST of U6 snRNA-associated Sm-like protein LSm4 vs. TrEMBL
Match: A0A159YQW1 (U6 snRNA-associated Sm-like protein LSm4 OS=Antheraea yamamai OX=7121 GN=LSM4 PE=2 SV=1)

HSP 1 Score: 129.028 bits (323), Expect = 4.014e-35
Identity = 56/93 (60.22%), Postives = 74/93 (79.57%), Query Frame = 2 
Query:   41 MLPLSLIKACINKPILVELKNNETYNGHLAIVDSFMNIQLKKAICTSKDGDKFWSIPEVYIKGNTIKFIRIPPDVIDNVNEEVKLKAPPRTTS 319
            MLPLSL++   N P+LVELKN ETYNGHL   D++MNI L++ ICTS+DGDKFW +PE YI+G+TIK++RIP +VID V EE ++KA  R+ +
Sbjct:    1 MLPLSLLRTAQNHPMLVELKNGETYNGHLVSCDNWMNINLREVICTSRDGDKFWRMPECYIRGSTIKYLRIPDEVIDMVKEETQVKARGRSEA 93
[Close]
BLAST of U6 snRNA-associated Sm-like protein LSm4 vs. TrEMBL
Match: A0A4S2KRI4 (U6 snRNA-associated Sm-like protein LSm4 OS=Opisthorchis felineus OX=147828 GN=LSM4 PE=3 SV=1)

HSP 1 Score: 129.413 bits (324), Expect = 4.051e-35
Identity = 58/97 (59.79%), Postives = 76/97 (78.35%), Query Frame = 2 
Query:   41 MLPLSLIKACINKPILVELKNNETYNGHLAIVDSFMNIQLKKAICTSKDGDKFWSIPEVYIKGNTIKFIRIPPDVIDNVNEEVKL-KAPPRTTSGSS 328
            MLP++L+KA IN P+LVELK+ ETYNGHLA  D +MNI LK+ ICTS+DGD+FW +PE YI+G+ IK++RIP DVID V E+++L KA  R+ S   
Sbjct:    1 MLPITLLKAAINLPMLVELKSGETYNGHLAACDDWMNIHLKEVICTSRDGDRFWRMPECYIRGSIIKYLRIPDDVIDKVKEDIQLAKARSRSQSADG 97
[Close]
BLAST of U6 snRNA-associated Sm-like protein LSm4 vs. TrEMBL
Match: A0A0D2PV30 (U6 snRNA-associated Sm-like protein LSm4 OS=Gossypium raimondii OX=29730 GN=LSM4 PE=3 SV=1)

HSP 1 Score: 127.487 bits (319), Expect = 4.838e-35
Identity = 58/100 (58.00%), Postives = 73/100 (73.00%), Query Frame = 2 
Query:   41 MLPLSLIKACINKPILVELKNNETYNGHLAIVDSFMNIQLKKAICTSKDGDKFWSIPEVYIKGNTIKFIRIPPDVIDNVNEEVKLKA---PPRTTSGSSQ 331
            MLPLSL+K     P+LVELKN ETYNGHL   D++MNI L++ ICTSKDGD+FW +PE YI+GNTIK++R+P +VID V EE K +A   PP    G  +
Sbjct:    1 MLPLSLLKTAQGHPMLVELKNGETYNGHLVNCDTWMNIHLREVICTSKDGDRFWRMPECYIRGNTIKYLRVPDEVIDKVQEETKSRADRKPPVLGGGRGR 100
[Close]
BLAST of U6 snRNA-associated Sm-like protein LSm4 vs. TrEMBL
Match: A0A2A3E6W1 (U6 snRNA-associated Sm-like protein LSm4 OS=Apis cerana cerana OX=94128 GN=LSM4 PE=3 SV=1)

HSP 1 Score: 128.642 bits (322), Expect = 5.197e-35
Identity = 56/90 (62.22%), Postives = 73/90 (81.11%), Query Frame = 2 
Query:   41 MLPLSLIKACINKPILVELKNNETYNGHLAIVDSFMNIQLKKAICTSKDGDKFWSIPEVYIKGNTIKFIRIPPDVIDNVNEEVKLKAPPR 310
            MLPLSL++   N P+LVELKN ETYNGHL   DS+MNI L++ ICTS+DGDKFW +PE YI+G+TIK++RIP +VID V E+V++K+  R
Sbjct:    1 MLPLSLLRTAQNHPMLVELKNGETYNGHLVSCDSWMNINLREVICTSRDGDKFWRMPECYIRGSTIKYLRIPDEVIDMVKEDVQMKSRGR 90
[Close]
BLAST of U6 snRNA-associated Sm-like protein LSm4 vs. Ensembl Cavefish
Match: lsm4 (LSM4 homolog, U6 small nuclear RNA and mRNA degradation associated [Source:NCBI gene;Acc:103043545])

HSP 1 Score: 124.405 bits (311), Expect = 1.055e-36
Identity = 56/90 (62.22%), Postives = 70/90 (77.78%), Query Frame = 2 
Query:   41 MLPLSLIKACINKPILVELKNNETYNGHLAIVDSFMNIQLKKAICTSKDGDKFWSIPEVYIKGNTIKFIRIPPDVIDNVNEEVKLKAPPR 310
            MLPLSL+K   N P+LVELKN ETYNGHL   D++MNI L++ ICTS+DGDKFW +PE YI+G+TIK++RIP ++ID V EEV  K   R
Sbjct:    1 MLPLSLLKTAQNHPMLVELKNGETYNGHLVSCDNWMNINLREVICTSRDGDKFWRMPECYIRGSTIKYLRIPDEIIDMVKEEVLSKGRGR 90
[Close]
BLAST of U6 snRNA-associated Sm-like protein LSm4 vs. Ensembl Cavefish
Match: lsm4 (LSM4 homolog, U6 small nuclear RNA and mRNA degradation associated [Source:NCBI gene;Acc:103043545])

HSP 1 Score: 60.4622 bits (145), Expect = 4.869e-12
Identity = 30/58 (51.72%), Postives = 37/58 (63.79%), Query Frame = 2 
Query:  158 LKKAICTSKDGDKFWSIPEVYIKGNTIKFIRIPPDVIDNVNEEVKLKAPPRTTSGSSQ 331
            LK A     DGDKFW +PE YI+G+TIK++RIP ++ID V EEV  K   R   G  Q
Sbjct:   22 LKTAQNHPMDGDKFWRMPECYIRGSTIKYLRIPDEIIDMVKEEVLSKG--RGRGGMQQ 77
[Close]
BLAST of U6 snRNA-associated Sm-like protein LSm4 vs. Ensembl Cavefish
Match: si:ch73-14h10.2 (LSM2 homolog, U6 small nuclear RNA and mRNA degradation associated [Source:NCBI gene;Acc:103032596])

HSP 1 Score: 49.6766 bits (117), Expect = 2.673e-8
Identity = 26/81 (32.10%), Postives = 48/81 (59.26%), Query Frame = 2 
Query:   41 MLPLSLIKACINKPILVELKNNETYNGHLAIVDSFMNIQLKKAICTSKDGDKF---WSIPEVYIKGNTIKFIRIPPDVIDN 274
            ML  S  K+ + K ++VELKN+ +  G L  VD ++NI+L     T  D +K+    S+   +I+G+ ++++++P D +D 
Sbjct:    1 MLFYSFFKSLVGKDVVVELKNDLSICGTLHSVDQYLNIKLTDISVT--DPEKYPHMLSVKNCFIRGSVVRYVQLPADEVDT 79
[Close]
BLAST of U6 snRNA-associated Sm-like protein LSm4 vs. Ensembl Yeast
Match: LSM2 (Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress [Source:SGD;Acc:S000000122])

HSP 1 Score: 51.6026 bits (122), Expect = 7.861e-10
Identity = 24/78 (30.77%), Postives = 46/78 (58.97%), Query Frame = 2 
Query:   41 MLPLSLIKACINKPILVELKNNETYNGHLAIVDSFMNIQLKKAICT-SKDGDKFWSIPEVYIKGNTIKFIRIPPDVID 271
            ML  S  K  +++ ++VELKN+    G L  VD F+N++L    CT  K      S+  ++I+G+T++++ +  +++D
Sbjct:    1 MLFFSFFKTLVDQEVVVELKNDIEIKGTLQSVDQFLNLKLDNISCTDEKKYPHLGSVRNIFIRGSTVRYVYLNKNMVD 78
[Close]
BLAST of U6 snRNA-associated Sm-like protein LSm4 vs. Ensembl Yeast
Match: LSM4 (Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; forms cytoplasmic foci upon DNA replication stress [Source:SGD;Acc:S000000914])

HSP 1 Score: 51.9878 bits (123), Expect = 2.567e-9
Identity = 31/93 (33.33%), Postives = 52/93 (55.91%), Query Frame = 2 
Query:   41 MLPLSLIKACINKPILVELKNNETYNGHLAIVDSFMNIQL-------KKAICTSKD---GDKFWSIPEVYIKGNTIKFIRIPPDVIDNVNEEV 289
            MLPL L+     + + +ELKN E   G L  VD++MN+ L       +++   S+D     K   + E+YI+G  IKFI++  ++ID V +++
Sbjct:    1 MLPLYLLTNAKGQQMQIELKNGEIIQGILTNVDNWMNLTLSNVTEYSEESAINSEDNAESSKAVKLNEIYIRGTFIKFIKLQDNIIDKVKQQI 93
[Close]
BLAST of U6 snRNA-associated Sm-like protein LSm4 vs. Ensembl Yeast
Match: SMD3 (Core Sm protein Sm D3; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D3 [Source:SGD;Acc:S000004137])

HSP 1 Score: 44.669 bits (104), Expect = 3.307e-7
Identity = 23/77 (29.87%), Postives = 41/77 (53.25%), Query Frame = 2 
Query:   44 LPLSLIKACINKPILVELKNNETYNGHLAIVDSFMNIQLKKAICTSKDGDKFWSIPEVYIKGNTIKFIRIPPDVIDN 274
            +P+ L+       + +EL    TY G L   +  MN+QL+  I T   G     + +++++G+ IKFI + PD++ N
Sbjct:    6 IPVKLLNEAQGHIVSLELTTGATYRGKLVESEDSMNVQLRDVIATEPQG-AVTHMDQIFVRGSQIKFIVV-PDLLKN 80
[Close]
BLAST of U6 snRNA-associated Sm-like protein LSm4 vs. Ensembl Nematostella
Match: EDO45138 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RTS4])

HSP 1 Score: 120.553 bits (301), Expect = 1.314e-35
Identity = 53/84 (63.10%), Postives = 68/84 (80.95%), Query Frame = 2 
Query:   41 MLPLSLIKACINKPILVELKNNETYNGHLAIVDSFMNIQLKKAICTSKDGDKFWSIPEVYIKGNTIKFIRIPPDVIDNVNEEVK 292
            +LPLSL++   N P+LVELKN ETYNGHL   D++MNI L++ ICTS+DGDKFW IPE Y++G+ IK++RIP +VID V EEVK
Sbjct:    2 VLPLSLLRTAQNHPMLVELKNGETYNGHLVSCDTWMNINLREVICTSRDGDKFWKIPECYVRGSNIKYLRIPDEVIDMVKEEVK 85
[Close]
BLAST of U6 snRNA-associated Sm-like protein LSm4 vs. Ensembl Nematostella
Match: EDO34663 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SNR1])

HSP 1 Score: 51.9878 bits (123), Expect = 1.618e-9
Identity = 26/80 (32.50%), Postives = 49/80 (61.25%), Query Frame = 2 
Query:   41 MLPLSLIKACINKPILVELKNNETYNGHLAIVDSFMNIQLKKAICTSKDGDKF---WSIPEVYIKGNTIKFIRIPPDVID 271
            ML  S  K+ + K ++VELKN+ + +G L  VD F+N++L     T  D DK+    S+   +I+G+ ++++++P + +D
Sbjct:    1 MLFYSFFKSLVGKEVIVELKNDLSVHGTLDSVDQFLNLKLTDIDVT--DPDKYPHMLSVKNCFIRGSVVRYVQLPSEEVD 78
[Close]
BLAST of U6 snRNA-associated Sm-like protein LSm4 vs. Ensembl Nematostella
Match: EDO47464 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RM92])

HSP 1 Score: 44.2838 bits (103), Expect = 1.539e-6
Identity = 22/78 (28.21%), Postives = 42/78 (53.85%), Query Frame = 2 
Query:   44 LPLSLIKACINKPILVELKNNETYNGHLAIVDSFMNIQLKKAICTSKDGDKFWSIPEVYIKGNTIKFIRIPPDVIDNV 277
            +P+ ++       I +E  N E Y G L   +  MN Q+     T++DG +   + +V+++G+ I+F+ I PD++ N 
Sbjct:    5 VPIKILHEAEGHVITLETLNGEVYRGKLIEAEDNMNCQMSNITVTARDG-RVSQLEQVFVRGSKIRFL-ILPDMLKNA 80
[Close]
BLAST of U6 snRNA-associated Sm-like protein LSm4 vs. Ensembl Nematostella
Match: EDO38707 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SC68])

HSP 1 Score: 42.743 bits (99), Expect = 6.102e-6
Identity = 22/70 (31.43%), Postives = 37/70 (52.86%), Query Frame = 2 
Query:   56 LIKACINKPILVELKNNETYNGHLAIVDSFMNIQLKKAICTSKDGDKFWSIPEVYIKGNTIKFIRIPPDV 265
            LIKA       VEL+N     G +  VD FMNI++K        G+   ++P +++ G  I+++ IP ++
Sbjct:   16 LIKAVQGYNTTVELRNESYLEGFIEHVDGFMNIKMKDVKFVKASGE-VDNLPAMFVVGTQIRYVHIPDEI 84
[Close]
BLAST of U6 snRNA-associated Sm-like protein LSm4 vs. Ensembl Medaka
Match: lsm4 (LSM4 homolog, U6 small nuclear RNA and mRNA degradation associated [Source:NCBI gene;Acc:101168448])

HSP 1 Score: 124.405 bits (311), Expect = 1.162e-36
Identity = 57/97 (58.76%), Postives = 73/97 (75.26%), Query Frame = 2 
Query:   41 MLPLSLIKACINKPILVELKNNETYNGHLAIVDSFMNIQLKKAICTSKDGDKFWSIPEVYIKGNTIKFIRIPPDVIDNVNEEVKLKAPPRTTSGSSQ 331
            MLPLSL+K   N P+LVELKN ETYNGHL   D++MNI L++ ICTS+DGDKFW +PE YI+G+TIK++RIP ++ID V +EV  K   R   G+ Q
Sbjct:    1 MLPLSLLKTAQNHPMLVELKNGETYNGHLVSCDNWMNINLREVICTSRDGDKFWRMPECYIRGSTIKYLRIPDEIIDMVKDEVVSKG--RGRGGAQQ 95
[Close]
BLAST of U6 snRNA-associated Sm-like protein LSm4 vs. Ensembl Medaka
Match: smx5 (LSM2 homolog, U6 small nuclear RNA and mRNA degradation associated [Source:NCBI gene;Acc:101162034])

HSP 1 Score: 49.6766 bits (117), Expect = 2.656e-8
Identity = 26/81 (32.10%), Postives = 48/81 (59.26%), Query Frame = 2 
Query:   41 MLPLSLIKACINKPILVELKNNETYNGHLAIVDSFMNIQLKKAICTSKDGDKF---WSIPEVYIKGNTIKFIRIPPDVIDN 274
            ML  S  K+ + K ++VELKN+ +  G L  VD ++NI+L     T  D +K+    S+   +I+G+ ++++++P D +D 
Sbjct:    1 MLFYSFFKSLVGKDVVVELKNDLSICGTLHSVDQYLNIKLTDISVT--DPEKYPHMLSVKNCFIRGSVVRYVQLPADEVDT 79
[Close]
BLAST of U6 snRNA-associated Sm-like protein LSm4 vs. Planmine SMEST
Match: SMESG000053212.1 (SMESG000053212.1)

HSP 1 Score: 134.035 bits (336), Expect = 4.579e-41
Identity = 63/63 (100.00%), Postives = 63/63 (100.00%), Query Frame = 2 
Query:  146 MNIQLKKAICTSKDGDKFWSIPEVYIKGNTIKFIRIPPDVIDNVNEEVKLKAPPRTTSGSSQF 334
            MNIQLKKAICTSKDGDKFWSIPEVYIKGNTIKFIRIPPDVIDNVNEEVKLKAPPRTTSGSSQF
Sbjct:    1 MNIQLKKAICTSKDGDKFWSIPEVYIKGNTIKFIRIPPDVIDNVNEEVKLKAPPRTTSGSSQF 63
The following BLAST results are available for this feature:
BLAST of U6 snRNA-associated Sm-like protein LSm4 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
LSM47.452e-3663.22LSM4 homolog, U6 small nuclear RNA and mRNA degrad... [more]
LSM41.605e-3563.22LSM4 homolog, U6 small nuclear RNA and mRNA degrad... [more]
LSM42.116e-2963.01LSM4 homolog, U6 small nuclear RNA and mRNA degrad... [more]
LSM49.883e-1652.38LSM4 homolog, U6 small nuclear RNA and mRNA degrad... [more]
LSM24.856e-832.10LSM2 homolog, U6 small nuclear RNA and mRNA degrad... [more]
back to top
BLAST of U6 snRNA-associated Sm-like protein LSm4 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 4
Match NameE-valueIdentityDescription
lsm-44.731e-3060.24Probable U6 snRNA-associated Sm-like protein LSm4 ... [more]
lsm-44.731e-3060.24Probable U6 snRNA-associated Sm-like protein LSm4 ... [more]
gut-21.555e-830.86U6 snRNA-associated Sm-like protein LSm2 [Source:... [more]
snr-11.629e-629.49Small nuclear ribonucleoprotein Sm D3 [Source:Uni... [more]
back to top
BLAST of U6 snRNA-associated Sm-like protein LSm4 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 3
Match NameE-valueIdentityDescription
CG177683.037e-3761.54gene:FBgn0032240 transcript:FBtr0100253[more]
CG177683.037e-3761.54gene:FBgn0032240 transcript:FBtr0304111[more]
CG104186.438e-933.33gene:FBgn0036277 transcript:FBtr0075954[more]
back to top
BLAST of U6 snRNA-associated Sm-like protein LSm4 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
lsm49.665e-3762.22LSM4 homolog, U6 small nuclear RNA and mRNA degrad... [more]
lsm42.806e-3061.84LSM4 homolog, U6 small nuclear RNA and mRNA degrad... [more]
smx52.754e-832.10smx5 [Source:ZFIN;Acc:ZDB-GENE-000616-10][more]
LSM22.754e-832.10si:ch73-14h10.2 [Source:ZFIN;Acc:ZDB-GENE-141222-6... [more]
smx52.100e-731.25smx5 [Source:ZFIN;Acc:ZDB-GENE-000616-10][more]
back to top
BLAST of U6 snRNA-associated Sm-like protein LSm4 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 3
Match NameE-valueIdentityDescription
nenf5.943e-3562.22Xenopus tropicalis LSM4 homolog, U6 small nuclear ... [more]
nenf3.049e-3062.67Xenopus tropicalis LSM4 homolog, U6 small nuclear ... [more]
nipa24.178e-832.10NIPA magnesium transporter 2 [Source:Xenbase;Acc:X... [more]
back to top
BLAST of U6 snRNA-associated Sm-like protein LSm4 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Lsm41.540e-3563.86LSM4 homolog, U6 small nuclear RNA and mRNA degrad... [more]
Lsm41.540e-3563.86LSM4 homolog, U6 small nuclear RNA and mRNA degrad... [more]
Lsm41.700e-2959.04LSM4 homolog, U6 small nuclear RNA and mRNA degrad... [more]
Lsm41.700e-2959.04LSM4 homolog, U6 small nuclear RNA and mRNA degrad... [more]
Lsm41.279e-1762.50LSM4 homolog, U6 small nuclear RNA and mRNA degrad... [more]
back to top
BLAST of U6 snRNA-associated Sm-like protein LSm4 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q43582|LSM4_TOBAC4.334e-3661.63Probable U6 snRNA-associated Sm-like protein LSm4 ... [more]
sp|Q9ZRU9|LSM4_FAGSY7.829e-3659.78Probable U6 snRNA-associated Sm-like protein LSm4 ... [more]
sp|Q9QXA5|LSM4_MOUSE2.068e-3558.76U6 snRNA-associated Sm-like protein LSm4 OS=Mus mu... [more]
sp|Q9LGE6|LSM4_ORYSJ2.090e-3565.06Probable U6 snRNA-associated Sm-like protein LSm4 ... [more]
sp|F4K4E3|LSM4_ARATH3.277e-3564.63Sm-like protein LSM4 OS=Arabidopsis thaliana OX=37... [more]
back to top
BLAST of U6 snRNA-associated Sm-like protein LSm4 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A3Q2VHI02.613e-3559.22U6 snRNA-associated Sm-like protein LSm4 OS=Haploc... [more]
A0A159YQW14.014e-3560.22U6 snRNA-associated Sm-like protein LSm4 OS=Anther... [more]
A0A4S2KRI44.051e-3559.79U6 snRNA-associated Sm-like protein LSm4 OS=Opisth... [more]
A0A0D2PV304.838e-3558.00U6 snRNA-associated Sm-like protein LSm4 OS=Gossyp... [more]
A0A2A3E6W15.197e-3562.22U6 snRNA-associated Sm-like protein LSm4 OS=Apis c... [more]
back to top
BLAST of U6 snRNA-associated Sm-like protein LSm4 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 3
Match NameE-valueIdentityDescription
lsm41.055e-3662.22LSM4 homolog, U6 small nuclear RNA and mRNA degrad... [more]
lsm44.869e-1251.72LSM4 homolog, U6 small nuclear RNA and mRNA degrad... [more]
si:ch73-14h10.22.673e-832.10LSM2 homolog, U6 small nuclear RNA and mRNA degrad... [more]
back to top
BLAST of U6 snRNA-associated Sm-like protein LSm4 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of U6 snRNA-associated Sm-like protein LSm4 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 3
Match NameE-valueIdentityDescription
LSM27.861e-1030.77Lsm (Like Sm) protein; part of heteroheptameric co... [more]
LSM42.567e-933.33Lsm (Like Sm) protein; part of heteroheptameric co... [more]
SMD33.307e-729.87Core Sm protein Sm D3; part of heteroheptameric co... [more]
back to top
BLAST of U6 snRNA-associated Sm-like protein LSm4 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 4
Match NameE-valueIdentityDescription
EDO451381.314e-3563.10Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO346631.618e-932.50Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO474641.539e-628.21Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO387076.102e-631.43Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
back to top
BLAST of U6 snRNA-associated Sm-like protein LSm4 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2
Match NameE-valueIdentityDescription
lsm41.162e-3658.76LSM4 homolog, U6 small nuclear RNA and mRNA degrad... [more]
smx52.656e-832.10LSM2 homolog, U6 small nuclear RNA and mRNA degrad... [more]
back to top
BLAST of U6 snRNA-associated Sm-like protein LSm4 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000053212.14.579e-41100.00SMESG000053212.1[more]
back to top
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30029609 ID=SMED30029609|Name=U6 snRNA-associated Sm-like protein LSm4|organism=Schmidtea mediterranea sexual|type=transcript|length=508bp
CATATATAGTATTTAATTTAGAAATTTTAAATTTATTAAAATGTTACCTC
TCAGTTTAATTAAAGCATGTATCAATAAACCAATTTTAGTTGAATTGAAA
AATAATGAAACGTATAATGGTCATTTGGCTATTGTAGATAGCTTTATGAA
TATTCAGTTAAAAAAGGCAATTTGCACGTCTAAGGACGGTGACAAATTTT
GGAGCATACCTGAAGTTTATATTAAAGGCAATACAATCAAATTCATCCGA
ATACCACCAGATGTTATTGACAACGTTAACGAAGAAGTGAAACTTAAAGC
CCCACCCCGCACAACATCTGGCTCATCTCAATTCAGAGGGGCAGGTAGAG
GGGGATATGCAGGGAATCAACAAGGTAGAGGAGGTCCTCGTGGTCGCGGA
TTTACTAATCGTGGTGCCCGTGGAGGTAGAGGTGGCAGGGGCATTTGAAT
AAATGTTTTTGTTTTGAATAATTTACACTTTACTAATTTATTTAATTTAA
GATTTTGG
back to top

protein sequence of SMED30029609-orf-1

>SMED30029609-orf-1 ID=SMED30029609-orf-1|Name=SMED30029609-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=136bp
MLPLSLIKACINKPILVELKNNETYNGHLAIVDSFMNIQLKKAICTSKDG
DKFWSIPEVYIKGNTIKFIRIPPDVIDNVNEEVKLKAPPRTTSGSSQFRG
AGRGGYAGNQQGRGGPRGRGFTNRGARGGRGGRGI*
back to top
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002109X1 cell
PLANA:0002111X2 cell
Vocabulary: INTERPRO
TermDefinition
IPR027141LSm4/Sm_D1/D3
IPR034101Lsm4
IPR001163LSM_dom_euk/arc
IPR010920LSM_dom_sf
Vocabulary: biological process
TermDefinition
GO:0000245spliceosomal complex assembly
GO:0000387spliceosomal snRNP assembly
GO:0000398mRNA splicing, via spliceosome
GO:0000956nuclear-transcribed mRNA catabolic process
GO:0006396RNA processing
GO:0006397mRNA processing
GO:0008380RNA splicing
GO:0033962cytoplasmic mRNA processing body assembly
GO:0043928exonucleolytic nuclear-transcribed mRNA catabolic process involved in deadenylation-dependent decay
Vocabulary: cellular component
TermDefinition
GO:0000932P-body
GO:0005634nucleus
GO:0005654nucleoplasm
GO:0005681spliceosomal complex
GO:0005688U6 snRNP
GO:0005829cytosol
GO:0030529intracellular ribonucleoprotein complex
GO:0043005neuron projection
GO:0097526spliceosomal tri-snRNP complex
Vocabulary: molecular function
TermDefinition
GO:0003723RNA binding
GO:0005515protein binding
GO:0017070U6 snRNA binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001163LSM domain, eukaryotic/archaea-typeSMARTSM00651Sm3coord: 5..71
e-value: 1.1E-17
score: 74.8
IPR001163LSM domain, eukaryotic/archaea-typePFAMPF01423LSMcoord: 6..70
e-value: 1.8E-16
score: 59.6
NoneNo IPR availableGENE3DG3DSA:2.30.30.100coord: 1..83
e-value: 6.9E-30
score: 104.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 86..135
NoneNo IPR availablePANTHERPTHR23338:SF34coord: 1..134
IPR027141Like-Sm (LSM) domain containing protein, LSm4/SmD1/SmD3PANTHERPTHR23338SMALL NUCLEAR RIBONUCLEOPROTEIN SMcoord: 1..134
IPR034101Sm-like protein Lsm4CDDcd01723LSm4coord: 2..77
e-value: 7.50799E-43
score: 133.855
IPR010920LSM domain superfamilySUPERFAMILYSSF50182Sm-like ribonucleoproteinscoord: 3..127