Dimer_Tnp_hAT domain-containing protein

Overview
NameDimer_Tnp_hAT domain-containing protein
Smed IDSMED30029589
Length (bp)1266
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Dimer_Tnp_hAT domain-containing protein (SMED30029589) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Dimer_Tnp_hAT domain-containing protein (SMED30029589) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30029589

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 10

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
protonephridiaSMED30029589h1SMcG0004893 dd_Smed_v4_11115_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30029589h1SMcG0004893 dd_Smed_v4_11115_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30029589h1SMcG0004893 dd_Smed_v4_11115_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30029589h1SMcG0004893 dd_Smed_v4_11115_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30029589h1SMcG0004893 dd_Smed_v4_11115_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30029589h1SMcG0004893 dd_Smed_v4_11115_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30029589h1SMcG0004893 dd_Smed_v4_11115_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30029589h1SMcG0004893 dd_Smed_v4_11115_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
enteric muscle cellSMED30029589h1SMcG0004892 dd_Smed_v4_19720_0_1dd_Smed_v4PMID:30471994
Scimone et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
enteric muscle cellSMED30029589h1SMcG0004893 dd_Smed_v4_19720_0_1dd_Smed_v4PMID:30471994
Scimone et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
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BLAST of Dimer_Tnp_hAT domain-containing protein vs. TrEMBL
Match: T1I3J9 (Dimer_Tnp_hAT domain-containing protein OS=Rhodnius prolixus OX=13249 PE=4 SV=1)

HSP 1 Score: 63.5438 bits (153), Expect = 1.019e-7
Identity = 32/78 (41.03%), Postives = 45/78 (57.69%), Query Frame = 2 
Query:  974 DVLKHLLQKAIDHKLTFQPQKDYQNAPSLIIKVH*ELIQFESDVVRDGEIERMYNFLLTISPTSVESERAYMYKKYMC 1207
            + LK  LQ AID  L   P   ++  PSL  K+  E+  FE    +   ++ +Y+FL+TI PTSVESERA+    Y+C
Sbjct:  127 NALKRKLQAAIDETLNTSPVPTFKKNPSLEQKIKDEITFFEKTGTKGTYLQNVYDFLMTIVPTSVESERAFSAAGYIC 204
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BLAST of Dimer_Tnp_hAT domain-containing protein vs. TrEMBL
Match: T1IEH5 (Dimer_Tnp_hAT domain-containing protein OS=Rhodnius prolixus OX=13249 PE=4 SV=1)

HSP 1 Score: 61.6178 bits (148), Expect = 1.803e-7
Identity = 32/78 (41.03%), Postives = 45/78 (57.69%), Query Frame = 2 
Query:  974 DVLKHLLQKAIDHKLTFQPQKDYQNAPSLIIKVH*ELIQFESDVVRDGEIERMYNFLLTISPTSVESERAYMYKKYMC 1207
            + LK  LQ AID  L   P   ++  PSL  K+  E+  FE    +   ++ +Y+FL+TI PTSVESERA+    Y+C
Sbjct:   29 NALKRKLQAAIDETLNTSPVPAFKKIPSLEQKIKDEITFFEKTETKGTYLQNVYDFLMTIVPTSVESERAFSAAGYIC 106
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BLAST of Dimer_Tnp_hAT domain-containing protein vs. TrEMBL
Match: T1HWC4 (Dimer_Tnp_hAT domain-containing protein OS=Rhodnius prolixus OX=13249 PE=4 SV=1)

HSP 1 Score: 63.1586 bits (152), Expect = 1.845e-7
Identity = 32/78 (41.03%), Postives = 45/78 (57.69%), Query Frame = 2 
Query:  974 DVLKHLLQKAIDHKLTFQPQKDYQNAPSLIIKVH*ELIQFESDVVRDGEIERMYNFLLTISPTSVESERAYMYKKYMC 1207
            + LK  LQ AID  L   P   ++  PSL  K+  E+  FE    +   ++ +Y+FL+TI PTSVESERA+    Y+C
Sbjct:  157 NALKRKLQAAIDETLNTSPVPAFKKIPSLEQKIKDEITFFEKTGTKGTYLQNVYDFLMTIVPTSVESERAFSAAGYIC 234
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BLAST of Dimer_Tnp_hAT domain-containing protein vs. TrEMBL
Match: T1HS18 (Dimer_Tnp_hAT domain-containing protein OS=Rhodnius prolixus OX=13249 PE=4 SV=1)

HSP 1 Score: 62.7734 bits (151), Expect = 3.558e-7
Identity = 32/78 (41.03%), Postives = 45/78 (57.69%), Query Frame = 2 
Query:  974 DVLKHLLQKAIDHKLTFQPQKDYQNAPSLIIKVH*ELIQFESDVVRDGEIERMYNFLLTISPTSVESERAYMYKKYMC 1207
            + LK  LQ AID  L   P   ++  PSL  K+  E+  FE    +   ++ +Y+FL+TI PTSVESERA+    Y+C
Sbjct:  190 NALKRKLQAAIDETLNTSPVPAFKKIPSLEQKIKDEITFFEKTGTKGTYLQNVYDFLMTIVPTSVESERAFSAAGYIC 267
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BLAST of Dimer_Tnp_hAT domain-containing protein vs. TrEMBL
Match: T1HY73 (Dimer_Tnp_hAT domain-containing protein OS=Rhodnius prolixus OX=13249 PE=4 SV=1)

HSP 1 Score: 60.4622 bits (145), Expect = 1.047e-6
Identity = 31/78 (39.74%), Postives = 45/78 (57.69%), Query Frame = 2 
Query:  974 DVLKHLLQKAIDHKLTFQPQKDYQNAPSLIIKVH*ELIQFESDVVRDGEIERMYNFLLTISPTSVESERAYMYKKYMC 1207
            + LK  LQ AID  L   P   ++  PSL  K+  E+  FE    +   ++ +Y+FL++I PTSVESERA+    Y+C
Sbjct:  120 NALKRKLQAAIDVTLNTSPVPAFKKIPSLEQKIKDEITFFEKTGTKGTYLQNVYDFLMSIVPTSVESERAFSAAGYIC 197
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BLAST of Dimer_Tnp_hAT domain-containing protein vs. Planmine SMEST
Match: SMESG000060444.1 (SMESG000060444.1)

HSP 1 Score: 350.903 bits (899), Expect = 1.308e-121
Identity = 169/175 (96.57%), Postives = 170/175 (97.14%), Query Frame = 1 
Query:  322 MNYKFKEYKKGIENNKSLWEYFFLERNGQVVKCKTCSKLINCTDESTSAFHSDFRTAHKITAVKRGFEDYGGISEISTNLYTPKNKTVESRCRQNASLLIADATFNFIPSHLHKGSGQLALDNKIELLYLIKEDETIYLAYFSLSITNTRWWVPKKIQMLIFSGNFQSINFYTYC 846
            MNYK KEYKKGIENNKSLWEYFFLERNGQVVKCKTCSKLINCTDESTSAFHSDFRTAHKITAVKRGFEDYGGISEISTNLYTPKNKTVES CRQNASLLIAD+TFNFIPSHLHK  GQLALDNKIELLY IKEDETIYLAYFSLSITNTRWWVPKKIQMLIFSGNFQSINFYTYC
Sbjct:    1 MNYKSKEYKKGIENNKSLWEYFFLERNGQVVKCKTCSKLINCTDESTSAFHSDFRTAHKITAVKRGFEDYGGISEISTNLYTPKNKTVESLCRQNASLLIADSTFNFIPSHLHKACGQLALDNKIELLYRIKEDETIYLAYFSLSITNTRWWVPKKIQMLIFSGNFQSINFYTYC 175
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BLAST of Dimer_Tnp_hAT domain-containing protein vs. Planmine SMEST
Match: SMESG000060886.1 (SMESG000060886.1)

HSP 1 Score: 317.39 bits (812), Expect = 1.176e-108
Identity = 153/158 (96.84%), Postives = 153/158 (96.84%), Query Frame = 1 
Query:  322 MNYKFKEYKKGIENNKSLWEYFFLERNGQVVKCKTCSKLINCTDESTSAFHSDFRTAHKITAVKRGFEDYGGISEISTNLYTPKNKTVESRCRQNASLLIADATFNFIPSHLHKGSGQLALDNKIELLYLIKEDETIYLAYFSLSITNTRWWVPKKIQ 795
            MNYKFKEYKKGIENNKSLWEYFFLERNGQVVKCKTCSKLINCTDESTSAFHSDFRTAHKITAVKRGFEDYGGISEISTNLYTPKNKTVES CRQNASLLIADATFNFIPSHLHK  GQLALDNKIELLY IKEDETIYL YFSLSITNTRWWVPKKIQ
Sbjct:    1 MNYKFKEYKKGIENNKSLWEYFFLERNGQVVKCKTCSKLINCTDESTSAFHSDFRTAHKITAVKRGFEDYGGISEISTNLYTPKNKTVESLCRQNASLLIADATFNFIPSHLHKACGQLALDNKIELLYRIKEDETIYLPYFSLSITNTRWWVPKKIQ 158
[Close]
BLAST of Dimer_Tnp_hAT domain-containing protein vs. Planmine SMEST
Match: SMESG000049212.1 (SMESG000049212.1)

HSP 1 Score: 41.9726 bits (97), Expect = 2.250e-6
Identity = 26/85 (30.59%), Postives = 42/85 (49.41%), Query Frame = 2 
Query:  953 IHEPSTADVLKHLLQKAIDHKLTFQPQKDYQNAPSLIIKVH*ELIQFESDVVRDGEIERMYNFLLTISPTSVESERAYMYKKYMC 1207
            ++E S ++ L   ++K        +P    +N  S   K+  E+  F+S   +   +++ YNFLL I PT VESER +     MC
Sbjct:   14 LNEKSISEQLNQAIEKGFIS--ASKPSTRVENTLS---KLQKEMPLFKSGGTKGDYLQKFYNFLLAIRPTIVESERVFSAAGLMC 93

HSP 2 Score: 28.4906 bits (62), Expect = 2.250e-6
Identity = 12/22 (54.55%), Postives = 16/22 (72.73%), Query Frame = 3 
Query: 1200 ICAKILSRQVDVTIDILCLMTS 1265
            +C+KI SR  D+TID LC + S
Sbjct:   92 MCSKIRSRLNDITIDALCFLRS 113
The following BLAST results are available for this feature:
BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Dimer_Tnp_hAT domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Dimer_Tnp_hAT domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
T1I3J91.019e-741.03Dimer_Tnp_hAT domain-containing protein OS=Rhodniu... [more]
T1IEH51.803e-741.03Dimer_Tnp_hAT domain-containing protein OS=Rhodniu... [more]
T1HWC41.845e-741.03Dimer_Tnp_hAT domain-containing protein OS=Rhodniu... [more]
T1HS183.558e-741.03Dimer_Tnp_hAT domain-containing protein OS=Rhodniu... [more]
T1HY731.047e-639.74Dimer_Tnp_hAT domain-containing protein OS=Rhodniu... [more]
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BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Dimer_Tnp_hAT domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3
Match NameE-valueIdentityDescription
SMESG000060444.11.308e-12196.57SMESG000060444.1[more]
SMESG000060886.11.176e-10896.84SMESG000060886.1[more]
SMESG000049212.12.250e-630.59SMESG000049212.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30029589 ID=SMED30029589|Name=Dimer_Tnp_hAT domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1266bp
TACCCACCGAACTCGTTGCCGATCTCCTGGGCTTTGTGGTGGCTTTTTAT
CAAGTAAATAGTTTGATTTATTACTTTTAACGGGATATTTATTTTCATAT
TCATGCTCTTTCGATTTATATTTCAAACAAATATCATCCATCAAACTATT
GTCGGACGAATTTCCGTCTGATGAACATGTTAAAAATCCCGAACTCGAAA
AATTCTTCTTTGTTTTCAGAAATTTTCCTGATTCACTAACAAAGCTACGA
TCCATGTTCTTCAAAACTGAGAAAATTGGAAGCCTAAGAAAACCCAATTA
AAGAGACAGACCATAATAGATATGAATTACAAATTCAAAGAATATAAGAA
AGGTATTGAAAACAATAAATCACTATGGGAATACTTTTTTCTGGAGAGAA
ATGGTCAAGTAGTGAAATGCAAAACATGTTCGAAACTTATTAACTGCACG
GATGAATCAACTAGTGCATTTCATTCGGATTTTCGAACGGCACACAAAAT
TACTGCTGTGAAACGAGGATTTGAAGATTATGGTGGAATCAGTGAAATAT
CCACAAATTTGTATACGCCCAAGAATAAAACTGTCGAATCACGTTGCCGA
CAAAATGCAAGCCTTCTGATAGCCGATGCGACTTTCAACTTTATACCCTC
ACATTTGCACAAAGGTAGTGGCCAGCTGGCACTTGATAATAAAATTGAGC
TATTATACCTAATTAAAGAAGATGAAACGATTTATTTAGCATACTTCAGT
CTCTCCATAACAAATACCAGGTGGTGGGTGCCAAAAAAAATTCAAATGCT
GATATTTTCTGGAAACTTCCAAAGCATCAATTTTTATACTTATTGTTAAA
ATTTTAGAAAAAATGTCTAACAAATCAATGAATGAAGAAATTTTATCAAG
AGATGACAATATTATCGAAGTATAAGATGAATTATCTTAGTACGTGGCAG
AAATACATGAACCATCAACAGCGGATGTGCTTAAGCATTTATTACAAAAA
GCTATTGACCATAAATTAACATTTCAACCACAGAAAGATTATCAAAATGC
ACCTAGTTTAATCATTAAAGTTCACTGAGAGTTGATCCAGTTTGAATCAG
ATGTGGTCAGAGACGGGGAAATCGAGAGAATGTACAATTTTCTTTTGACG
ATTTCACCGACAAGCGTAGAATCAGAAAGAGCTTATATGTACAAAAAATA
TATGTGCAAAAATTCTATCACGTCAAGTTGATGTCACAATAGACATATTA
TGTTTAATGACATCGC
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protein sequence of SMED30029589-orf-1

>SMED30029589-orf-1 ID=SMED30029589-orf-1|Name=SMED30029589-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=176bp
MNYKFKEYKKGIENNKSLWEYFFLERNGQVVKCKTCSKLINCTDESTSAF
HSDFRTAHKITAVKRGFEDYGGISEISTNLYTPKNKTVESRCRQNASLLI
ADATFNFIPSHLHKGSGQLALDNKIELLYLIKEDETIYLAYFSLSITNTR
WWVPKKIQMLIFSGNFQSINFYTYC*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:0046983protein dimerization activity
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0003104enteric muscle cell