General transcription factor II-I repeat domain-containing protein 2

Overview
NameGeneral transcription factor II-I repeat domain-containing protein 2
Smed IDSMED30029568
Length (bp)386
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of General transcription factor II-I repeat domain-containing protein 2 (SMED30029568) t-SNE clustered cells

Violin plots show distribution of expression levels for General transcription factor II-I repeat domain-containing protein 2 (SMED30029568) in cells (dots) of each of the 12 neoblast clusters.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30029568

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30029568h1SMcG0000827 dd_Smed_v4_10733_1_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30029568h1SMcG0000827 dd_Smed_v4_10733_1_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30029568h1SMcG0000827 dd_Smed_v4_10733_1_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of General transcription factor II-I repeat domain-containing protein 2 vs. Ensembl Human
Match: GTF2IRD2B (GTF2I repeat domain containing 2B [Source:HGNC Symbol;Acc:HGNC:33125])

HSP 1 Score: 45.8246 bits (107), Expect = 5.374e-6
Identity = 35/84 (41.67%), Postives = 48/84 (57.14%), Query Frame = 3 
Query:    3 EIINLQCNNELEQVHKTTSKFEFYNTYITPERYPNLRLFAQKIVSAFGSTYVCEAXXXXXXXXXXXXRASLTDENLESQLRCAT 254
            E+I+LQCN  L+  +      EFY  Y+    YP  +    KI+S FGSTY+CE  FS MK +K+K+ + L D   +S L  AT
Sbjct:  868 EVIDLQCNTVLKTKYDKVGIPEFYK-YLW-GSYPKYKHHCAKILSMFGSTYICEQLFSIMKLSKTKYCSQLKDSQWDSVLHIAT 949
[Close]
BLAST of General transcription factor II-I repeat domain-containing protein 2 vs. Ensembl Human
Match: GTF2IRD2 (GTF2I repeat domain containing 2 [Source:HGNC Symbol;Acc:HGNC:30775])

HSP 1 Score: 45.8246 bits (107), Expect = 5.426e-6
Identity = 35/84 (41.67%), Postives = 48/84 (57.14%), Query Frame = 3 
Query:    3 EIINLQCNNELEQVHKTTSKFEFYNTYITPERYPNLRLFAQKIVSAFGSTYVCEAXXXXXXXXXXXXRASLTDENLESQLRCAT 254
            E+I+LQCN  L+  +      EFY  Y+    YP  +    KI+S FGSTY+CE  FS MK +K+K+ + L D   +S L  AT
Sbjct:  868 EVIDLQCNTVLKTKYDKVGIPEFYK-YLW-GSYPKYKHHCAKILSMFGSTYICEQLFSIMKLSKTKYCSQLKDSQWDSVLHIAT 949
[Close]
BLAST of General transcription factor II-I repeat domain-containing protein 2 vs. Ensembl Human
Match: GTF2IRD2B (GTF2I repeat domain containing 2B [Source:HGNC Symbol;Acc:HGNC:33125])

HSP 1 Score: 45.4394 bits (106), Expect = 6.221e-6
Identity = 35/84 (41.67%), Postives = 48/84 (57.14%), Query Frame = 3 
Query:    3 EIINLQCNNELEQVHKTTSKFEFYNTYITPERYPNLRLFAQKIVSAFGSTYVCEAXXXXXXXXXXXXRASLTDENLESQLRCAT 254
            E+I+LQCN  L+  +      EFY  Y+    YP  +    KI+S FGSTY+CE  FS MK +K+K+ + L D   +S L  AT
Sbjct: 1035 EVIDLQCNTVLKTKYDKVGIPEFYK-YLW-GSYPKYKHHCAKILSMFGSTYICEQLFSIMKLSKTKYCSQLKDSQWDSVLHIAT 1116
[Close]
BLAST of General transcription factor II-I repeat domain-containing protein 2 vs. Ensembl Zebrafish
Match: AL928808.1 (pep chromosome:GRCz11:20:17257101:17259573:-1 gene:ENSDARG00000101333.2 transcript:ENSDART00000166397.2 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:AL928808.1)

HSP 1 Score: 68.5514 bits (166), Expect = 4.181e-14
Identity = 48/105 (45.71%), Postives = 69/105 (65.71%), Query Frame = 3 
Query:    3 EIINLQCNNELEQVHKTTSKFEFYNTYITP-ERYPNLRLFAQKIVSAFGSTYVCEAXXXXXXXXXXXXRASLTDENLESQLRCATTKIDVDLKTLSDRKEKQISH 314
            E+I L  ++ L+ V +  +  EF+   I P ERYPN++  A K++S FGSTYVCE  FS +K  KSK R+ LTD +++  LR ATT+ + DLK + + KE Q+SH
Sbjct:  515 EMIELSEDDRLKSVLREGT-VEFWK--IVPVERYPNVKQAALKLLSMFGSTYVCELLFSTLKLVKSKHRSVLTDTHVKELLRVATTEYEPDLKKIVETKECQVSH 616
[Close]
BLAST of General transcription factor II-I repeat domain-containing protein 2 vs. Ensembl Zebrafish
Match: CR392001.3 (pep chromosome:GRCz11:8:38963323:38965260:-1 gene:ENSDARG00000117159.1 transcript:ENSDART00000181495.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:CR392001.3)

HSP 1 Score: 47.7506 bits (112), Expect = 6.026e-7
Identity = 34/82 (41.46%), Postives = 51/82 (62.20%), Query Frame = 3 
Query:   72 YNTY-ITPERYPNLRLFAQKIVSAFGSTYVCEAXXXXXXXXXXXXRASLTDENLESQLRCATTKIDVDLKTLSDRKEKQISH 314
            Y+T+   P+ Y N++ +A  ++S FGSTY+CE  FS M + KSK+R  LT E+L+S ++   T    D++ LS    KQ SH
Sbjct:  558 YDTWNALPDCYKNMKTYAFGVLSIFGSTYLCEQIFSNMNYIKSKYRTRLTHESLQSCVKIKVTSYMPDVEKLSSDVRKQKSH 639
[Close]
BLAST of General transcription factor II-I repeat domain-containing protein 2 vs. Ensembl Xenopus
Match: spag1 (sperm associated antigen 1 [Source:Xenbase;Acc:XB-GENE-853609])

HSP 1 Score: 52.7582 bits (125), Expect = 1.618e-8
Identity = 37/102 (36.27%), Postives = 57/102 (55.88%), Query Frame = 3 
Query:   12 NLQCNNELEQVHKTTSKFEFYNTYITPERYPNLRLFAQKIVSAFGSTYVCEAXXXXXXXXXXXXRASLTDENLESQLRCATTKIDVDLKTL-SDRKEKQISH 314
            + + N   + +H  + K   +   ++ E+YP LR F  ++ S FGSTY+CE  FS M F KSK+R S+ D  L+  +R +TT I  D+  L + +   Q SH
Sbjct:  484 DFEGNGGNQFLHSCSEKGVAFFKLLSQEQYPILRDFGMRMASMFGSTYICEKAFSDMGFIKSKYRNSICDSTLQQIIRISTTSISADIDELVAQQLHPQTSH 585
[Close]
BLAST of General transcription factor II-I repeat domain-containing protein 2 vs. Ensembl Xenopus
Match: ENSXETT00000016563.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:4:37379994:37381838:-1 gene:ENSXETG00000009412.1 transcript:ENSXETT00000016563.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 52.373 bits (124), Expect = 2.014e-8
Identity = 36/92 (39.13%), Postives = 53/92 (57.61%), Query Frame = 3 
Query:   42 VHKTTSKFEFYNTYITPERYPNLRLFAQKIVSAFGSTYVCEAXXXXXXXXXXXXRASLTDENLESQLRCATTKIDVDLKTL-SDRKEKQISH 314
            +H  + K   +   ++ E+YP LR F  ++ S FGSTY+CE  FS M F KSK+R S+ D  L+  +R +TT I  D+  L + +   Q SH
Sbjct:  523 LHSCSEKGVAFFKLLSQEQYPILRDFGMRMASMFGSTYICEKAFSDMGFIKSKYRNSICDSTLQQIIRISTTSISADIDELVAQQLHPQTSH 614
[Close]
BLAST of General transcription factor II-I repeat domain-containing protein 2 vs. Ensembl Mouse
Match: Epm2aip1 (EPM2A (laforin) interacting protein 1 [Source:MGI Symbol;Acc:MGI:1925031])

HSP 1 Score: 45.8246 bits (107), Expect = 3.463e-6
Identity = 28/88 (31.82%), Postives = 48/88 (54.55%), Query Frame = 3 
Query:    3 EIINLQCNNELEQVHKTTSKFEFYNTYITPERYPNLRLFAQKIVSAFGSTYVCEAXXXXXXXXXXXXRASLTDENLESQLRCATTKID 266
            E+  LQ N +L   ++     +FY   ++ E YP ++  A K+ S F S  +C+  F+ +  N+      LTDE+L++  R ATT++D
Sbjct:  507 ELTKLQANTDLWNEYRVKDLGQFYAG-LSGEAYPIIKGVAYKVASLFDSNQICDKAFAYLTRNQHTLSQPLTDEHLQALFRVATTEMD 593
[Close]
BLAST of General transcription factor II-I repeat domain-containing protein 2 vs. Ensembl Mouse
Match: Epm2aip1 (EPM2A (laforin) interacting protein 1 [Source:MGI Symbol;Acc:MGI:1925031])

HSP 1 Score: 45.8246 bits (107), Expect = 3.463e-6
Identity = 28/88 (31.82%), Postives = 48/88 (54.55%), Query Frame = 3 
Query:    3 EIINLQCNNELEQVHKTTSKFEFYNTYITPERYPNLRLFAQKIVSAFGSTYVCEAXXXXXXXXXXXXRASLTDENLESQLRCATTKID 266
            E+  LQ N +L   ++     +FY   ++ E YP ++  A K+ S F S  +C+  F+ +  N+      LTDE+L++  R ATT++D
Sbjct:  507 ELTKLQANTDLWNEYRVKDLGQFYAG-LSGEAYPIIKGVAYKVASLFDSNQICDKAFAYLTRNQHTLSQPLTDEHLQALFRVATTEMD 593
[Close]
BLAST of General transcription factor II-I repeat domain-containing protein 2 vs. TrEMBL
Match: A0A067RI84 (Uncharacterized protein (Fragment) OS=Zootermopsis nevadensis OX=136037 GN=L798_03511 PE=4 SV=1)

HSP 1 Score: 118.627 bits (296), Expect = 1.408e-30
Identity = 64/105 (60.95%), Postives = 86/105 (81.90%), Query Frame = 3 
Query:    3 EIINLQCNNELEQVHKTT-SKFEFYNTYITPERYPNLRLFAQKIVSAFGSTYVCEAXXXXXXXXXXXXRASLTDENLESQLRCATTKIDVDLKTLSDRKEKQISH 314
            E+++LQCN EL+ + +T+ SK +FYNTY+T E++ NLR  AQK+VSAFGSTY CE+FFSKMK  K K R+++TD NL+ QLRCA T+I++DL+ LS+R EKQISH
Sbjct:  122 ELVDLQCNIELKHIFETSESKIDFYNTYVTKEKFSNLRNLAQKVVSAFGSTYTCESFFSKMKLTKHKTRSNITDANLQHQLRCANTQIEIDLQKLSERVEKQISH 226
[Close]
BLAST of General transcription factor II-I repeat domain-containing protein 2 vs. TrEMBL
Match: A0A067R753 (Uncharacterized protein (Fragment) OS=Zootermopsis nevadensis OX=136037 GN=L798_11998 PE=4 SV=1)

HSP 1 Score: 92.0485 bits (227), Expect = 3.228e-20
Identity = 53/95 (55.79%), Postives = 74/95 (77.89%), Query Frame = 3 
Query:    3 EIINLQCNNELEQVHKTT-SKFEFYNTYITPERYPNLRLFAQKIVSAFGSTYVCEAXXXXXXXXXXXXRASLTDENLESQLRCATTKIDVDLKTL 284
            E+++LQCN EL+ + +T+ SK +FYNTY+T E++ NL   AQK+VSAFGSTY C +FFSKMK  K K R+++ D NL+ QLRCA T+I++DL+ L
Sbjct:  153 ELVDLQCNIELKHIFETSESKIDFYNTYVTKEKFSNL---AQKVVSAFGSTYTCVSFFSKMKLTKHKTRSNIMDANLQHQLRCANTQIEIDLQKL 244
[Close]
BLAST of General transcription factor II-I repeat domain-containing protein 2 vs. TrEMBL
Match: T1IBG8 (Dimer_Tnp_hAT domain-containing protein (Fragment) OS=Rhodnius prolixus OX=13249 PE=4 SV=1)

HSP 1 Score: 90.5077 bits (223), Expect = 1.413e-19
Identity = 50/104 (48.08%), Postives = 78/104 (75.00%), Query Frame = 3 
Query:    3 EIINLQCNNELEQVHKTTSKFEFYNTYITPERYPNLRLFAQKIVSAFGSTYVCEAXXXXXXXXXXXXRASLTDENLESQLRCATTKIDVDLKTLSDRKEKQISH 314
            E+I LQ + EL+ +++  +K E+Y  YI  +++PNL+  A  I+SAFG+TY CE+FFSK+   K+K+R+ L DEN+ +QLRCA+TK+ VD+K +S + +KQ+SH
Sbjct:  149 ELIELQSSIELKSLYEG-NKIEYYQKYILEDKFPNLKRLAMCIISAFGTTYRCESFFSKLNLVKTKYRSRLLDENMTNQLRCASTKLSVDIKKISSKIQKQVSH 251
[Close]
BLAST of General transcription factor II-I repeat domain-containing protein 2 vs. TrEMBL
Match: H3APJ3 (Dimer_Tnp_hAT domain-containing protein OS=Latimeria chalumnae OX=7897 PE=4 SV=1)

HSP 1 Score: 89.7373 bits (221), Expect = 1.929e-19
Identity = 51/104 (49.04%), Postives = 69/104 (66.35%), Query Frame = 3 
Query:    3 EIINLQCNNELEQVHKTTSKFEFYNTYITPERYPNLRLFAQKIVSAFGSTYVCEAXXXXXXXXXXXXRASLTDENLESQLRCATTKIDVDLKTLSDRKEKQISH 314
            E+I LQ N+EL   +   S  EFY  Y+  +++PNLR  A KIVS FG+TY CE FFSK+   K+  RA LTD++LE+QLR AT+ + VD+  L+  K+ Q SH
Sbjct:  123 EVIELQSNSELMAKYNNLSLLEFYRLYVDADKFPNLRRHALKIVSLFGTTYCCEQFFSKLSITKNHLRAKLTDDSLENQLRIATSSVPVDITRLTKEKQSQPSH 226
[Close]
BLAST of General transcription factor II-I repeat domain-containing protein 2 vs. TrEMBL
Match: H3A6C6 (Dimer_Tnp_hAT domain-containing protein OS=Latimeria chalumnae OX=7897 PE=4 SV=1)

HSP 1 Score: 88.5817 bits (218), Expect = 4.648e-19
Identity = 51/103 (49.51%), Postives = 68/103 (66.02%), Query Frame = 3 
Query:    3 EIINLQCNNELEQVHKTTSKFEFYNTYITPERYPNLRLFAQKIVSAFGSTYVCEAXXXXXXXXXXXXRASLTDENLESQLRCATTKIDVDLKTLSDRKEKQIS 311
            E+I LQ N+EL   +   S  EFY  Y+  +++PNLR  A KIVS FG+TY CE FFSK+   K+  RA LTD+NLE+QLR AT+ + VD+  L+  K+ Q S
Sbjct:  108 EVIELQSNSELMAKYNNLSLLEFYRLYVDADKFPNLRRHALKIVSLFGTTYCCEQFFSKLSIMKNHLRAKLTDDNLENQLRIATSSVPVDITRLTKEKQSQPS 210
[Close]
BLAST of General transcription factor II-I repeat domain-containing protein 2 vs. Ensembl Cavefish
Match: ENSAMXT00000037725.1 (pep primary_assembly:Astyanax_mexicanus-2.0:14:29162452:29166863:1 gene:ENSAMXG00000037303.1 transcript:ENSAMXT00000037725.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 76.6406 bits (187), Expect = 5.298e-17
Identity = 48/104 (46.15%), Postives = 64/104 (61.54%), Query Frame = 3 
Query:    3 EIINLQCNNELEQVHKTTSKFEFYNTYITPERYPNLRLFAQKIVSAFGSTYVCEAXXXXXXXXXXXXRASLTDENLESQLRCATTKIDVDLKTLSDRKEKQISH 314
            EII L+C+  +   H+     EFY + +  E++PNL   AQK +S FGSTY+CE  FS MK NKS  R  LTDENL++ LR ATT ++ D+  L   +   ISH
Sbjct:  685 EIIELKCSTAMRTKHREMPLLEFYQS-LDREQFPNLFANAQKWISMFGSTYICEQMFSLMKLNKSPLRTRLTDENLQAVLRLATTSLEPDINQLVSERRCNISH 787
[Close]
BLAST of General transcription factor II-I repeat domain-containing protein 2 vs. Ensembl Cavefish
Match: ENSAMXT00000030684.1 (pep primary_assembly:Astyanax_mexicanus-2.0:7:19673305:19679188:1 gene:ENSAMXG00000037520.1 transcript:ENSAMXT00000030684.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 75.8702 bits (185), Expect = 7.920e-17
Identity = 48/104 (46.15%), Postives = 63/104 (60.58%), Query Frame = 3 
Query:    3 EIINLQCNNELEQVHKTTSKFEFYNTYITPERYPNLRLFAQKIVSAFGSTYVCEAXXXXXXXXXXXXRASLTDENLESQLRCATTKIDVDLKTLSDRKEKQISH 314
            EII L C+  +   H+     EFY + +  E++PNL   AQK +S FGSTY+CE  FS MK NKS  R  LTDENL++ LR ATT ++ D+  L   +   ISH
Sbjct:  405 EIIELNCSTAMRTKHREMPLLEFYQS-LDREQFPNLFANAQKWISMFGSTYICEQMFSLMKLNKSPLRTRLTDENLQAVLRLATTSLEPDINQLVSERRCNISH 507
[Close]
BLAST of General transcription factor II-I repeat domain-containing protein 2 vs. Ensembl Cavefish
Match: ENSAMXT00000050833.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000549.1:45261:46892:1 gene:ENSAMXG00000036460.1 transcript:ENSAMXT00000050833.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 73.559 bits (179), Expect = 8.217e-17
Identity = 47/103 (45.63%), Postives = 63/103 (61.17%), Query Frame = 3 
Query:    3 EIINLQCNNELEQVHKTTSKFEFYNTYITPERYPNLRLFAQKIVSAFGSTYVCEAXXXXXXXXXXXXRASLTDENLESQLRCATTKIDVDLKTLSDRKEKQIS 311
            EII L+C+  +   H+     EFY + +  E++PNL   AQK +S FGSTY+CE  FS MK NKS  R  LTDENL++ LR ATT ++ D+  L   +   IS
Sbjct:  108 EIIELKCSTAMRTKHREMPLLEFYQS-LDREQFPNLFANAQKWISMFGSTYICEQMFSLMKLNKSPLRTRLTDENLQAVLRLATTSLEPDINQLVSERRFNIS 209
[Close]
BLAST of General transcription factor II-I repeat domain-containing protein 2 vs. Ensembl Cavefish
Match: ENSAMXT00000051559.1 (pep primary_assembly:Astyanax_mexicanus-2.0:7:19676892:19681451:1 gene:ENSAMXG00000037520.1 transcript:ENSAMXT00000051559.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 71.633 bits (174), Expect = 2.356e-15
Identity = 46/100 (46.00%), Postives = 61/100 (61.00%), Query Frame = 3 
Query:    3 EIINLQCNNELEQVHKTTSKFEFYNTYITPERYPNLRLFAQKIVSAFGSTYVCEAXXXXXXXXXXXXRASLTDENLESQLRCATTKIDVDLKTLSDRKEK 302
            EII L C+  +   H+     EFY + +  E++PNL   AQK +S FGSTY+CE  FS MK NKS  R  LTDENL++ LR ATT ++ D+  L   + K
Sbjct:  443 EIIELNCSTAMRTKHREMPLLEFYQS-LDREQFPNLFANAQKWISMFGSTYICEQMFSLMKLNKSPLRTRLTDENLQAVLRLATTSLEPDINQLVSERLK 541
[Close]
BLAST of General transcription factor II-I repeat domain-containing protein 2 vs. Ensembl Cavefish
Match: ENSAMXT00000036928.1 (pep primary_assembly:Astyanax_mexicanus-2.0:7:19677316:19680173:1 gene:ENSAMXG00000037520.1 transcript:ENSAMXT00000036928.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 71.633 bits (174), Expect = 2.497e-15
Identity = 45/94 (47.87%), Postives = 59/94 (62.77%), Query Frame = 3 
Query:    3 EIINLQCNNELEQVHKTTSKFEFYNTYITPERYPNLRLFAQKIVSAFGSTYVCEAXXXXXXXXXXXXRASLTDENLESQLRCATTKIDVDLKTL 284
            EII L C+  +   H+     EFY + +  E++PNL   AQK +S FGSTY+CE  FS MK NKS  R  LTDENL++ LR ATT ++ D+  L
Sbjct:  411 EIIELNCSTAMRTKHREMPLLEFYQS-LDREQFPNLFANAQKWISMFGSTYICEQMFSLMKLNKSPLRTRLTDENLQAVLRLATTSLEPDINQL 503
[Close]
BLAST of General transcription factor II-I repeat domain-containing protein 2 vs. Ensembl Medaka
Match: ENSORLT00000032235.1 (general transcription factor II-I repeat domain-containing protein 2-like [Source:NCBI gene;Acc:111947776])

HSP 1 Score: 62.003 bits (149), Expect = 2.017e-12
Identity = 39/105 (37.14%), Postives = 65/105 (61.90%), Query Frame = 3 
Query:    3 EIINLQCNNELEQVHKTTSKFEFYNTYITPERYPNLRLFAQKIVSAFGSTYVCEAXXXXXXXXXXXXRASLTDENLESQLRCATTKIDVDLKTLSDRKEK-QISH 314
            E+I  QC+ EL +   +    +FY  +++ +RYP +R  AQ ++S FGSTY+CE  FS M  NK K R +LTD +L+  L  + +K+  ++++L   K++  +SH
Sbjct:  113 ELIEFQCDTELRRKFVSLPLRDFY-PHVSKQRYPQMRKNAQVMLSLFGSTYICEQTFSLMNLNKIKLRGTLTDSHLQDILTLSVSKLQPNIQSLIKSKDQLHVSH 216
[Close]
BLAST of General transcription factor II-I repeat domain-containing protein 2 vs. Ensembl Medaka
Match: ENSORLT00000029843.1 (pep primary_assembly:ASM223467v1:2:5236691:5237599:1 gene:ENSORLG00000028650.1 transcript:ENSORLT00000029843.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 61.6178 bits (148), Expect = 2.630e-12
Identity = 39/105 (37.14%), Postives = 64/105 (60.95%), Query Frame = 3 
Query:    3 EIINLQCNNELEQVHKTTSKFEFYNTYITPERYPNLRLFAQKIVSAFGSTYVCEAXXXXXXXXXXXXRASLTDENLESQLRCATTKIDVDLKTLSDRKEK-QISH 314
            E+I  QC+ EL     +    +FY  +++ +RYP +R  AQ ++S FGSTY+CE  FS M  NK K R +LTD +L+  L  + +K+  ++++L   K++  +SH
Sbjct:  100 ELIEFQCDTELRPKFVSLPLRDFY-PHVSKQRYPQMRKNAQVMLSLFGSTYICEQTFSLMNLNKIKLRGTLTDSHLQDILTLSVSKLQPNIQSLIKSKDQLHVSH 203
[Close]
BLAST of General transcription factor II-I repeat domain-containing protein 2 vs. Ensembl Medaka
Match: ENSORLT00000027093.1 (general transcription factor II-I repeat domain-containing protein 2B-like [Source:NCBI gene;Acc:111947639])

HSP 1 Score: 62.003 bits (149), Expect = 5.547e-12
Identity = 43/91 (47.25%), Postives = 61/91 (67.03%), Query Frame = 3 
Query:    3 EIINLQCNNELEQVHKTTSKFEFYNTYITPERYPNLRLFAQKIVSAFGSTYVCEAXXXXXXXXXXXXRASLTDENLESQLRCATTKIDVDL 275
            E+I LQ +  L +  ++ S  +FY++ +  E +P+LR  AQKI+  FGSTY CE  FS MKFNKSK R+S+TD++L + LR AT+ I  D 
Sbjct:  480 ELIELQSDMLLAERFRSVSLLDFYSS-LKKENFPHLRRHAQKILVLFGSTYHCEQAFSVMKFNKSKHRSSVTDDHLSAVLRIATSDIQPDF 569
[Close]
BLAST of General transcription factor II-I repeat domain-containing protein 2 vs. Ensembl Medaka
Match: ENSORLT00000044825.1 (general transcription factor II-I repeat domain-containing protein 2-like [Source:NCBI gene;Acc:111949247])

HSP 1 Score: 61.6178 bits (148), Expect = 7.309e-12
Identity = 39/105 (37.14%), Postives = 65/105 (61.90%), Query Frame = 3 
Query:    3 EIINLQCNNELEQVHKTTSKFEFYNTYITPERYPNLRLFAQKIVSAFGSTYVCEAXXXXXXXXXXXXRASLTDENLESQLRCATTKIDVDLKTLSDRKEK-QISH 314
            E+I  QC+ EL +   +    +FY  +++ +RYP +R  AQ ++S FGSTY+CE  FS M  NK K R +LTD +L+  L  + +K+  ++++L   K++  +SH
Sbjct:  459 ELIEFQCDTELRRKFVSLPLRDFY-PHVSKQRYPQMRKNAQVMLSLFGSTYICEQTFSLMNLNKIKLRGTLTDSHLQDILTLSVSKLQPNIQSLIKSKDQLHVSH 562
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BLAST of General transcription factor II-I repeat domain-containing protein 2 vs. Ensembl Medaka
Match: ENSORLT00000038650.1 (pep primary_assembly:ASM223467v1:1:16225402:16226668:1 gene:ENSORLG00000028538.1 transcript:ENSORLT00000038650.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 59.6918 bits (143), Expect = 1.505e-11
Identity = 38/105 (36.19%), Postives = 64/105 (60.95%), Query Frame = 3 
Query:    3 EIINLQCNNELEQVHKTTSKFEFYNTYITPERYPNLRLFAQKIVSAFGSTYVCEAXXXXXXXXXXXXRASLTDENLESQLRCATTKIDVDLKTLSDRKEK-QISH 314
            E+I  QC+ EL +   +    +FY  +++ +RYP +R  AQ ++S FGSTY+CE  FS M  NK K R +LTD + +  L  + +K+  ++++L   K++  +SH
Sbjct:  112 ELIEFQCDTELRRKFVSLPLRDFY-PHVSVQRYPQMRKNAQVMLSLFGSTYMCEQTFSLMNLNKIKLRGTLTDSHWQDILTLSVSKLQPNIQSLMKSKDQLHVSH 215
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BLAST of General transcription factor II-I repeat domain-containing protein 2 vs. Planmine SMEST
Match: SMESG000015269.1 (SMESG000015269.1)

HSP 1 Score: 66.6254 bits (161), Expect = 1.170e-13
Identity = 43/104 (41.35%), Postives = 68/104 (65.38%), Query Frame = 3 
Query:    3 EIINLQCNNELEQVHKTTSKFEFYNTYITPERYPNLRLFAQKIVSAFGSTYVCEAXXXXXXXXXXXXRASLTDENLESQLRCATTKIDVDLKTLSDRKEKQISH 314
            EII+LQ N +++  +K  +  +FY  Y+  + +PNL+ FA+K +S F +TYVCE  F +MK+ KSK+RA+L+D++L+S L    T  D + K +  +K KQ  H
Sbjct:  475 EIIDLQANEQIKDKYKEGNLIDFYK-YLDAKEFPNLKKFARKYISMFETTYVCEQTFPRMKYLKSKYRANLSDDHLQSLLMIGVTNFDPNYKDILQQK-KQFHH 576
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BLAST of General transcription factor II-I repeat domain-containing protein 2 vs. Planmine SMEST
Match: SMESG000042994.1 (SMESG000042994.1)

HSP 1 Score: 65.0846 bits (157), Expect = 1.303e-13
Identity = 41/97 (42.27%), Postives = 65/97 (67.01%), Query Frame = 3 
Query:    6 IINLQCNNELEQVHKTTSKFEFYNTYITPERYPNLRLFAQKIVSAFGSTYVCEAXXXXXXXXXXXXRASLTDENLESQLRCATTKIDVDLKTLSDRK 296
            II+LQ N +++  +K  +  +FY  Y+  + +PNL+ FA K +S FG+TYVCE  FS+MK+ KSK+RA+L+D++L+S L    T  D + K +  +K
Sbjct:  118 IIDLQANEQIKYKYKEGNLIDFYK-YLDAKEFPNLKKFACKFISMFGTTYVCEQTFSRMKYLKSKYRANLSDDHLQSLLMIGVTNFDPNYKDILQQK 213
[Close]
BLAST of General transcription factor II-I repeat domain-containing protein 2 vs. Planmine SMEST
Match: SMESG000022722.1 (SMESG000022722.1)

HSP 1 Score: 55.0694 bits (131), Expect = 1.653e-10
Identity = 44/104 (42.31%), Postives = 65/104 (62.50%), Query Frame = 3 
Query:    3 EIINLQCNNELEQVHKTTSKFEFYNTYITPERYPNLRLFAQKIVSAFGSTYVCEAXXXXXXXXXXXXRASLTDENLESQLRCATTKIDVDLKTLSDRKEKQISH 314
            E+I++Q + EL     + ++ EF+ +++TP + P LR  A KI   F STY+CE+ FS MKF K+K+R  LT+ENL + L+ A T    D+K L D K+   SH
Sbjct:   30 ELISIQNDIEL-SCEFSKNQLEFW-SHVTPNQNPFLRDVALKISCLFVSTYLCESVFSNMKFIKNKYRRKLTNENLGNGLKLAITDYSPDMKKLLDEKQFYSSH 131
[Close]
BLAST of General transcription factor II-I repeat domain-containing protein 2 vs. Planmine SMEST
Match: SMESG000045419.1 (SMESG000045419.1)

HSP 1 Score: 56.6102 bits (135), Expect = 3.800e-10
Identity = 41/103 (39.81%), Postives = 60/103 (58.25%), Query Frame = 3 
Query:    3 EIINLQCNNELEQVHKTTSKFEFYNTYITPERYPNLRLFAQKIVSAFGSTYVCEAXXXXXXXXXXXXRASLTDENLESQLRCATTKIDVDLKTLSDRKEKQIS 311
            EI+ LQ N  L+      +   FY     P++Y  L  FA+K++ AFGSTY+CE  FS M F K+KF + LTDE+L + +R  T+ +  D+  L+  K+ Q S
Sbjct:  440 EILQLQHNEILKNAFLLENIQHFYRCL--PKQYEGLISFAKKMIVAFGSTYICEQEFSAMSFRKNKFSSQLTDEHLNASIRICTSGLKADIDNLAKDKQPQKS 540
[Close]
BLAST of General transcription factor II-I repeat domain-containing protein 2 vs. Planmine SMEST
Match: SMESG000042366.1 (SMESG000042366.1)

HSP 1 Score: 52.7582 bits (125), Expect = 2.746e-9
Identity = 38/94 (40.43%), Postives = 58/94 (61.70%), Query Frame = 3 
Query:    3 EIINLQCNNELEQVHKTTSKFEFYNTYITPERYPNLRLFAQKIVSAFGSTYVCEAXXXXXXXXXXXXRASLTDENLESQLRCATTKIDVDLKTL 284
            E+I+LQ +N L++  K      FY + +  + +PN++ FA K++  F STY+CE  FS M  NKSK+R+ LTD NL+S +R +T+    D   L
Sbjct:   87 ELIDLQNDNILKENFKEMELTNFYAS-LKNDNFPNIQQFAMKMLVLFASTYICEQTFSCMNINKSKYRSQLTDTNLDSIIRISTSTFTPDYGKL 179
The following BLAST results are available for this feature:
BLAST of General transcription factor II-I repeat domain-containing protein 2 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 3
Match NameE-valueIdentityDescription
GTF2IRD2B5.374e-641.67GTF2I repeat domain containing 2B [Source:HGNC Sym... [more]
GTF2IRD25.426e-641.67GTF2I repeat domain containing 2 [Source:HGNC Symb... [more]
GTF2IRD2B6.221e-641.67GTF2I repeat domain containing 2B [Source:HGNC Sym... [more]
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BLAST of General transcription factor II-I repeat domain-containing protein 2 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of General transcription factor II-I repeat domain-containing protein 2 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of General transcription factor II-I repeat domain-containing protein 2 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
AL928808.14.181e-1445.71pep chromosome:GRCz11:20:17257101:17259573:-1 gene... [more]
CR392001.36.026e-741.46pep chromosome:GRCz11:8:38963323:38965260:-1 gene:... [more]
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BLAST of General transcription factor II-I repeat domain-containing protein 2 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2
Match NameE-valueIdentityDescription
spag11.618e-836.27sperm associated antigen 1 [Source:Xenbase;Acc:XB-... [more]
ENSXETT00000016563.12.014e-839.13pep primary_assembly:Xenopus_tropicalis_v9.1:4:373... [more]
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BLAST of General transcription factor II-I repeat domain-containing protein 2 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Epm2aip13.463e-631.82EPM2A (laforin) interacting protein 1 [Source:MGI ... [more]
Epm2aip13.463e-631.82EPM2A (laforin) interacting protein 1 [Source:MGI ... [more]
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BLAST of General transcription factor II-I repeat domain-containing protein 2 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of General transcription factor II-I repeat domain-containing protein 2 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A067RI841.408e-3060.95Uncharacterized protein (Fragment) OS=Zootermopsis... [more]
A0A067R7533.228e-2055.79Uncharacterized protein (Fragment) OS=Zootermopsis... [more]
T1IBG81.413e-1948.08Dimer_Tnp_hAT domain-containing protein (Fragment)... [more]
H3APJ31.929e-1949.04Dimer_Tnp_hAT domain-containing protein OS=Latimer... [more]
H3A6C64.648e-1949.51Dimer_Tnp_hAT domain-containing protein OS=Latimer... [more]
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BLAST of General transcription factor II-I repeat domain-containing protein 2 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSAMXT00000037725.15.298e-1746.15pep primary_assembly:Astyanax_mexicanus-2.0:14:291... [more]
ENSAMXT00000030684.17.920e-1746.15pep primary_assembly:Astyanax_mexicanus-2.0:7:1967... [more]
ENSAMXT00000050833.18.217e-1745.63pep primary_assembly:Astyanax_mexicanus-2.0:APWO02... [more]
ENSAMXT00000051559.12.356e-1546.00pep primary_assembly:Astyanax_mexicanus-2.0:7:1967... [more]
ENSAMXT00000036928.12.497e-1547.87pep primary_assembly:Astyanax_mexicanus-2.0:7:1967... [more]
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BLAST of General transcription factor II-I repeat domain-containing protein 2 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of General transcription factor II-I repeat domain-containing protein 2 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of General transcription factor II-I repeat domain-containing protein 2 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of General transcription factor II-I repeat domain-containing protein 2 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSORLT00000032235.12.017e-1237.14general transcription factor II-I repeat domain-co... [more]
ENSORLT00000029843.12.630e-1237.14pep primary_assembly:ASM223467v1:2:5236691:5237599... [more]
ENSORLT00000027093.15.547e-1247.25general transcription factor II-I repeat domain-co... [more]
ENSORLT00000044825.17.309e-1237.14general transcription factor II-I repeat domain-co... [more]
ENSORLT00000038650.11.505e-1136.19pep primary_assembly:ASM223467v1:1:16225402:162266... [more]
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BLAST of General transcription factor II-I repeat domain-containing protein 2 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000015269.11.170e-1341.35SMESG000015269.1[more]
SMESG000042994.11.303e-1342.27SMESG000042994.1[more]
SMESG000022722.11.653e-1042.31SMESG000022722.1[more]
SMESG000045419.13.800e-1039.81SMESG000045419.1[more]
SMESG000042366.12.746e-940.43SMESG000042366.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30029568 ID=SMED30029568|Name=General transcription factor II-I repeat domain-containing protein 2|organism=Schmidtea mediterranea sexual|type=transcript|length=386bp
TGGAAATAATCAATTTGCAGTGTAACAATGAGTTGGAGCAGGTTCATAAA
ACGACATCAAAATTTGAGTTCTATAACACGTATATAACACCAGAAAGGTA
TCCTAACTTAAGATTATTTGCTCAAAAAATTGTAAGCGCCTTCGGATCTA
CATATGTTTGTGAAGCCTTTTTTTCAAAAATGAAATTTAATAAAAGTAAA
TTTCGCGCTTCATTAACAGACGAAAACCTTGAAAGCCAATTACGATGTGC
AACTACCAAAATTGATGTAGATTTAAAAACATTGAGCGATCGCAAAGAAA
AGCAAATATCCCATTAATAAATGTTATACTTAAGTGTTCTGAATTAATAA
AAATAAATGGTTCAATAAAAATGTTCTTGAAACAAT
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protein sequence of SMED30029568-orf-1

>SMED30029568-orf-1 ID=SMED30029568-orf-1|Name=SMED30029568-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=105bp
EIINLQCNNELEQVHKTTSKFEFYNTYITPERYPNLRLFAQKIVSAFGST
YVCEAFFSKMKFNKSKFRASLTDENLESQLRCATTKIDVDLKTLSDRKEK
QISH*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: INTERPRO
TermDefinition
IPR008906HATC_C_dom
IPR012337RNaseH-like_sf
Vocabulary: molecular function
TermDefinition
GO:0046983protein dimerization activity
GO:0003676nucleic acid binding