SMED30029559

Overview
NameSMED30029559
Smed IDSMED30029559
Length (bp)3503
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30029559 (SMED30029559) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30029559 (SMED30029559) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30029559 (SMED30029559) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30029559 (SMED30029559) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30029559

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 1

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30029559SMESG000072318.1 SmedASXL_000047SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of SMED30029559 vs. Planmine SMEST
Match: SMESG000072318.1 (SMESG000072318.1)

HSP 1 Score: 1472.6 bits (3811), Expect = 0.000e+0
Identity = 744/826 (90.07%), Postives = 750/826 (90.80%), Query Frame = 1
Query:  319 MTQQIYRVLVLLVLMTLKINVELEFSIYDMLFSCHAIINQQKYTARKFVLAKDLIVGPVTIFNDSAGSCGYAPQIIDVQVECIFHGQSFVIGQKTKTYGLNPTELYYFENIKHNRSEITFSHVFLNFTIKCLCSLTCFLNPFSLETYLKFQYPRKNASFLKIDPFPILLKTEITCSLEIQIPFESITWNCGFCQSFSNNYLSEICKSIKYNGNKIVINMDNIFAPNLSCLCVCIADEIYPPYMNLPMFIPSENLVIELYPLPINPYSNLTCNASLPFPFLMWECIFNNQFFELGKNIIFSNNNDKEMYYSLIVYQNKLLIYYFENNQSIKCRCIVGRENYQMEVFRKYFDLPIQVMPKISLIPPIPAIIQDLQCYYSDYFSLLVYNWGCYLSKGNKVDRTFILSLRQNENTLLLDYGSLNSIITCFCEIEFRFQKIIQIGPRAYFILPETGFLLYIFSYSN*IKNLDKLKISPSNPTIFETLTCISRSKKLYKFNWYCNLDNFLVSINYPVNINYLSDYSKYLVRNIKIDSMNRMIFKLDFQLKNITCTCEVFYTSIYFSNQLLNFPLPISDYPDLFIIPSVFAYGNIITCQTNKSRFFEYFWKCIYQNVTVYLGTKPPPRIGEKIKYFYSVFIVNKNSLLAHPIWTEVDIYCECYFRFISLLSFSKKTAKFILPKSGLPLFRLMQLNCSNPCDYNAYTHYAVQQSGELNYVSIAPYSKFIIFSKELFFYQEFTSFKFNRFFPLIHIKNITSLKCEKLEWILLWKTTNETIARGFWLLKIFKMSRITNISTNLPYHNIAYTFDFKFFCDLINIYNKILIVAFTINN 2796
            MTQQIYRVLVLLVLMTLKINVELEFSIYDMLFSCHAIINQQKYTARKFVLAKDLIVGPVTIFNDSAGSCGYAPQIIDVQVECIFHGQSFVIGQKTKTYGLNPTELYYFENIKHNRSEITFSHVFLNFTIKCLCSLTCFLNPFSLETYLKFQYPRKNASFLKIDPFPILLKTEITCSLEIQIPFESITWNCGFCQSFSNNYLSEICKSIKYNGNKIVINMDNIFAPNLSCLCVCIADEIYPPYMNLPMFIPSENLVIELYPLPINPYSNLTCNASLPFPFLMWECIFNNQFFEL                   VYQNKLLIYYFENNQSIKCRC VGRENYQMEVFRKYFDLPIQVMPKISLIPPIPAIIQDLQCYYSDYFSLLVYNWGCYLSKGNKVDRTFILSLRQNENTLLLDYGSLNSIITCFCEIEFRFQKIIQIGPRAYFILPET                DKLKISPSNPTIFETLTCISRSKKLYKFNWYCNLDNFLVSINYPVNINYLSDYSKYLVRNIKIDSMNRMIFKLDFQLKNITCTCEVFYTSIYFS QLLNFPLPISDYPDLFIIPSVFAYGNIITCQTNKSRFFE+  +   QN             GE                  + I+  +DIYCECYFRFISLLSFSKK+AKFILPKSGLPLFRLMQLNCSNPCDYNAYTHYAVQQSGELNYVSIAPYSKFIIFSKELFFYQEFTSFKFNRFFPLIHIKNITSLKCEKLEWILLWKTTNETIARGFWLLKIFKMSRITNISTNLPYHNIAYTFDFKFFCDLINIYNKILIVAFTINN
Sbjct:    1 MTQQIYRVLVLLVLMTLKINVELEFSIYDMLFSCHAIINQQKYTARKFVLAKDLIVGPVTIFNDSAGSCGYAPQIIDVQVECIFHGQSFVIGQKTKTYGLNPTELYYFENIKHNRSEITFSHVFLNFTIKCLCSLTCFLNPFSLETYLKFQYPRKNASFLKIDPFPILLKTEITCSLEIQIPFESITWNCGFCQSFSNNYLSEICKSIKYNGNKIVINMDNIFAPNLSCLCVCIADEIYPPYMNLPMFIPSENLVIELYPLPINPYSNLTCNASLPFPFLMWECIFNNQFFEL-------------------VYQNKLLIYYFENNQSIKCRCTVGRENYQMEVFRKYFDLPIQVMPKISLIPPIPAIIQDLQCYYSDYFSLLVYNWGCYLSKGNKVDRTFILSLRQNENTLLLDYGSLNSIITCFCEIEFRFQKIIQIGPRAYFILPET----------------DKLKISPSNPTIFETLTCISRSKKLYKFNWYCNLDNFLVSINYPVNINYLSDYSKYLVRNIKIDSMNRMIFKLDFQLKNITCTCEVFYTSIYFSTQLLNFPLPISDYPDLFIIPSVFAYGNIITCQTNKSRFFEH--QATSQN------------WGE------------------NKIFLFIDIYCECYFRFISLLSFSKKSAKFILPKSGLPLFRLMQLNCSNPCDYNAYTHYAVQQSGELNYVSIAPYSKFIIFSKELFFYQEFTSFKFNRFFPLIHIKNITSLKCEKLEWILLWKTTNETIARGFWLLKIFKMSRITNISTNLPYHNIAYTFDFKFFCDLINIYNKILIVAFTINN 759          
BLAST of SMED30029559 vs. Planmine SMEST
Match: SMESG000072318.1 (SMESG000072318.1)

HSP 1 Score: 973.77 bits (2516), Expect = 0.000e+0
Identity = 513/654 (78.44%), Postives = 541/654 (82.72%), Query Frame = 1
Query: 1060 FIPSENLVIELYPLPINPYSNLTCNASLPFPFLMWECIFNNQFFELGKNIIFSNNNDKEMYYSLIVYQNKLLIYYFEN--NQSIKC---------RCIVGRENYQMEVFRKYFDLPIQVMPKISLIPPIPAIIQDLQCYYSDYFSLLVYNWGCYLSKGNKVDRTFILSLRQNENTLLLDYGSLNSIITCFCEIEFRFQKIIQIGPRAYFILPETGFLLYIFSYSN*IKNLDKLKISPSNPTIFETLTCISRSKKLYKFNWYCNLDNFLVSINYPVNINYLSDYSKYLVRNIKIDSMNRMIFKLDFQLKNITCTCEVFYTSIYFSNQLLNFPLPISDYPDLFIIPSVFAYGNIITCQTNKSRFFEYFWKCIYQNVTVYLGTKPPPRIGEKIKYFYSVFIVNKNSLLAHPIWTEVDIYCECYFRFISLLSFSKKTAKFILPKSGLPLFRLMQLNCSNPCDYNAYTHYAVQQSGELNYVSIAPYSKFIIFSKELFFYQEFTSFKFNRFFPLIHIKNITSLKCEKLEWILLWKTTNETIARGFWLLKIFKMSRITNISTNLPYHNIAYTFDFKFFCDLINIYNKILIVAFTINNGMLMDFFYVINNTIMINTLIPKKDSILITCHIYIYDQKFPVLCRMYRISNLSKILVFQSYKNIN 2988
            F+ +++L++   P+ I   S  +C  +     +  ECIF+ Q F +G+       N  E+YY   +  N+  I +     N +IKC         +C VGRENYQMEVFRKYFDLPIQVMPKISLIPPIPAIIQDLQCYYSDYFSLLVYNWGCYLSKGNKVDRTFILSLRQNENTLLLDYGSLNSIITCFCEIEFRFQKIIQIGPRAYFILPET                DKLKISPSNPTIFETLTCISRSKKLYKFNWYCNLDNFLVSINYPVNINYLSDYSKYLVRNIKIDSMNRMIFKLDFQLKNITCTCEVFYTSIYFS QLLNFPLPISDYPDLFIIPSVFAYGNIITCQTNKSRFFE+  +   QN             GE                  + I+  +DIYCECYFRFISLLSFSKK+AKFILPKSGLPLFRLMQLNCSNPCDYNAYTHYAVQQSGELNYVSIAPYSKFIIFSKELFFYQEFTSFKFNRFFPLIHIKNITSLKCEKLEWILLWKTTNETIARGFWLLKIFKMSRITNISTNLPYHNIAYTFDFKFFCDLINIYNKILIVAFTINNGMLMD+FYVINNTIMINTLIPKKDSILITCHIYIYDQKFPVLCRMYRISNLSKILVFQ +K I+
Sbjct:   57 FVLAKDLIVG--PVTIFNDSAGSCGYAPQIIDVQVECIFHGQSFVIGQKTKTYGLNPTELYYFENIKHNRSEITFSHVFLNFTIKCLCSLTLSKKKCTVGRENYQMEVFRKYFDLPIQVMPKISLIPPIPAIIQDLQCYYSDYFSLLVYNWGCYLSKGNKVDRTFILSLRQNENTLLLDYGSLNSIITCFCEIEFRFQKIIQIGPRAYFILPET----------------DKLKISPSNPTIFETLTCISRSKKLYKFNWYCNLDNFLVSINYPVNINYLSDYSKYLVRNIKIDSMNRMIFKLDFQLKNITCTCEVFYTSIYFSTQLLNFPLPISDYPDLFIIPSVFAYGNIITCQTNKSRFFEH--QATSQNW------------GE------------------NKIFLFIDIYCECYFRFISLLSFSKKSAKFILPKSGLPLFRLMQLNCSNPCDYNAYTHYAVQQSGELNYVSIAPYSKFIIFSKELFFYQEFTSFKFNRFFPLIHIKNITSLKCEKLEWILLWKTTNETIARGFWLLKIFKMSRITNISTNLPYHNIAYTFDFKFFCDLINIYNKILIVAFTINNGMLMDYFYVINNTIMINTLIPKKDSILITCHIYIYDQKFPVLCRMYRISNLSKILVFQFHKVIS 660          

HSP 2 Score: 291.197 bits (744), Expect = 1.671e-84
Identity = 214/488 (43.85%), Postives = 272/488 (55.74%), Query Frame = 1
Query:  292 MLNFEYKWIMTQQIYRVLVLLVLMTLKINVELEFSIYDMLFSCHAIINQQKYTARKFVLAKDLIVGPVTIFNDSAGSCGYAPQIIDVQVECIFHGQSFVIGQKTKTYGLNPTELYYFENIKHNRSEITFSHVFLNFTIKCLCSLT-----CFLNP--FSLETYLK-FQYPRKNASFLK-IDPFPILLKTEITCSLEIQIPFESITWNCGFCQSFSNNYLSEICKSIKYNGNKIVINMDNIFAPNLSCLCVCIADEIYPPYMNLPMFIPSENLVI-ELYPLPINP-----YSNLTC-NASLPFPFLMWECIFNNQFFELG--KNIIFSNNNDKEMYYSLIVYQNKLLIYYFENN-QSIKCRCIVGRENYQMEVFRKYFDLPIQVMPKISLIPPIPAIIQDLQCYY--SDYFS--LLVYNWGCYLSKGNKVDRTFILSLRQNENTLLLDYGSLNSIITCFCEIEFRFQKIIQIGPR-AYFILPETGFLLY 1683
            MLNFEYKWIMTQQIYRVLVLLVLMTLKINVELEFSIYDMLFSCHAIINQQKYTARKFVLAKDLIVGPVTIFNDSAGSCGYAPQIIDVQVECIFHGQSFVIGQKTKTYGLNPTELYYFENIKHNRSEITFSHVFLNFTIKCLCSLT     C +    + +E + K F  P +    +  I P P +++ ++ C            W C   +   N        S++ N N ++++  ++ +  ++C C     EI   +  +    P    ++ E   L I+P     +  LTC + S       W C  +N    +    NI + ++  K +  ++ +     +I+  +   ++I C C V   +         F LPI   P + +IP + A    + C    S +F       NWG                    EN + L        I  +CE  FRF  ++    + A FILP++G  L+
Sbjct:    1 MLNFEYKWIMTQQIYRVLVLLVLMTLKINVELEFSIYDMLFSCHAIINQQKYTARKFVLAKDLIVGPVTIFNDSAGSCGYAPQIIDVQVECIFHGQSFVIGQKTKTYGLNPTELYYFENIKHNRSEITFSHVFLNFTIKCLCSLTLSKKKCTVGRENYQMEVFRKYFDLPIQVMPKISLIPPIPAIIQ-DLQCYYSDYFSLLVYNWGCYLSK--GNKVDRTFILSLRQNENTLLLDYGSLNSI-ITCFC-----EIEFRFQKIIQIGPRAYFILPETDKLKISPSNPTIFETLTCISRSKKLYKFNWYCNLDNFLVSINYPVNINYLSDYSKYLVRNIKIDSMNRMIFKLDFQLKNITCTCEVFYTSIYFSTQLLNFPLPISDYPDLFIIPSVFAYGNIITCQTNKSRFFEHQATSQNWG--------------------ENKIFL-------FIDIYCECYFRFISLLSFSKKSAKFILPKSGLPLF 452          
BLAST of SMED30029559 vs. Planmine SMEST
Match: SMESG000072318.1 (SMESG000072318.1)

HSP 1 Score: 967.992 bits (2501), Expect = 0.000e+0
Identity = 506/643 (78.69%), Postives = 530/643 (82.43%), Query Frame = 1
Query: 1060 FIPSENLVIELYPLPINPYSNLTCNASLPFPFLMWECIFNNQFFELGKNIIFSNNNDKEMYYSLIVYQNKLLIYYFENNQSIKCRCIVGRENYQMEVFRKYFDLPIQVMPKISLIPPIPAIIQDLQCYYSDYFSLLVYNWGCYLSKGNKVDRTFILSLRQNENTLLLDYGSLNSIITCFCEIEFRFQKIIQIGPRAYFILPETGFLLYIFSYSN*IKNLDKLKISPSNPTIFETLTCISRSKKLYKFNWYCNLDNFLVSINYPVNINYLSDYSKYLVRNIKIDSMNRMIFKLDFQLKNITCTCEVFYTSIYFSNQLLNFPLPISDYPDLFIIPSVFAYGNIITCQTNKSRFFEYFWKCIYQNVTVYLGTKPPPRIGEKIKYFYSVFIVNKNSLLAHPIWTEVDIYCECYFRFISLLSFSKKTAKFILPKSGLPLFRLMQLNCSNPCDYNAYTHYAVQQSGELNYVSIAPYSKFIIFSKELFFYQEFTSFKFNRFFPLIHIKNITSLKCEKLEWILLWKTTNETIARGFWLLKIFKMSRITNISTNLPYHNIAYTFDFKFFCDLINIYNKILIVAFTINNGMLMDFFYVINNTIMINTLIPKKDSILITCHIYIYDQKFPVLCRMYRISNLSKILVFQSYKNIN 2988
            F+ +++L++   P+ I   S  +C  +     +  ECIF+ Q F +G+          + Y S                   K +C VGRENYQMEVFRKYFDLPIQVMPKISLIPPIPAIIQDLQCYYSDYFSLLVYNWGCYLSKGNKVDRTFILSLRQNENTLLLDYGSLNSIITCFCEIEFRFQKIIQIGPRAYFILPET                DKLKISPSNPTIFETLTCISRSKKLYKFNWYCNLDNFLVSINYPVNINYLSDYSKYLVRNIKIDSMNRMIFKLDFQLKNITCTCEVFYTSIYFS QLLNFPLPISDYPDLFIIPSVFAYGNIITCQTNKSRFFE+  +   QN             GE                  + I+  +DIYCECYFRFISLLSFSKK+AKFILPKSGLPLFRLMQLNCSNPCDYNAYTHYAVQQSGELNYVSIAPYSKFIIFSKELFFYQEFTSFKFNRFFPLIHIKNITSLKCEKLEWILLWKTTNETIARGFWLLKIFKMSRITNISTNLPYHNIAYTFDFKFFCDLINIYNKILIVAFTINNGMLMD+FYVINNTIMINTLIPKKDSILITCHIYIYDQKFPVLCRMYRISNLSKILVFQ +K I+
Sbjct:   48 FVLAKDLIVG--PVTIFNDSAGSCGYAPQIIDVQVECIFHGQSFVIGQKT--------KTYVS-------------------KKKCTVGRENYQMEVFRKYFDLPIQVMPKISLIPPIPAIIQDLQCYYSDYFSLLVYNWGCYLSKGNKVDRTFILSLRQNENTLLLDYGSLNSIITCFCEIEFRFQKIIQIGPRAYFILPET----------------DKLKISPSNPTIFETLTCISRSKKLYKFNWYCNLDNFLVSINYPVNINYLSDYSKYLVRNIKIDSMNRMIFKLDFQLKNITCTCEVFYTSIYFSTQLLNFPLPISDYPDLFIIPSVFAYGNIITCQTNKSRFFEH--QATSQN------------WGE------------------NKIFLFIDIYCECYFRFISLLSFSKKSAKFILPKSGLPLFRLMQLNCSNPCDYNAYTHYAVQQSGELNYVSIAPYSKFIIFSKELFFYQEFTSFKFNRFFPLIHIKNITSLKCEKLEWILLWKTTNETIARGFWLLKIFKMSRITNISTNLPYHNIAYTFDFKFFCDLINIYNKILIVAFTINNGMLMDYFYVINNTIMINTLIPKKDSILITCHIYIYDQKFPVLCRMYRISNLSKILVFQFHKVIS 613          

HSP 2 Score: 202.216 bits (513), Expect = 8.613e-53
Identity = 98/98 (100.00%), Postives = 98/98 (100.00%), Query Frame = 1
Query:  319 MTQQIYRVLVLLVLMTLKINVELEFSIYDMLFSCHAIINQQKYTARKFVLAKDLIVGPVTIFNDSAGSCGYAPQIIDVQVECIFHGQSFVIGQKTKTY 612
            MTQQIYRVLVLLVLMTLKINVELEFSIYDMLFSCHAIINQQKYTARKFVLAKDLIVGPVTIFNDSAGSCGYAPQIIDVQVECIFHGQSFVIGQKTKTY
Sbjct:    1 MTQQIYRVLVLLVLMTLKINVELEFSIYDMLFSCHAIINQQKYTARKFVLAKDLIVGPVTIFNDSAGSCGYAPQIIDVQVECIFHGQSFVIGQKTKTY 98          
BLAST of SMED30029559 vs. Planmine SMEST
Match: SMESG000072318.1 (SMESG000072318.1)

HSP 1 Score: 846.655 bits (2186), Expect = 0.000e+0
Identity = 447/579 (77.20%), Postives = 468/579 (80.83%), Query Frame = 1
Query: 1060 FIPSENLVIELYPLPINPYSNLTCNASLPFPFLMWECIFNNQFFELGKNIIFSNNNDKEMYYSLIVYQNKLLIYYFENNQSIKCRCIVGRENYQMEVFRKYFDLPIQVMPKISLIPPIPAIIQDLQCYYSDYFSLLVYNWGCYLSKGNKVDRTFILSLRQNENTLLLDYGSLNSIITCFCEIEFRFQKIIQIGPRAYFILPETGFLLYIFSYSN*IKNLDKLKISPSNPTIFETLTCISRSKKLYKFNWYCNLDNFLVSINYPVNINYLSDYSKYLVRNIKIDSMNRMIFKLDFQLKNITCTCEVFYTSIYFSNQLLNFPLPISDYPDLFIIPSVFAYGNIITCQTNKSRFFEYFWKCIYQNVTVYLGTKPPPRIGEKIKYFYSVFIVNKNSLLAHPIWTEVDIYCECYFRFISLLSFSKKTAKFILPKSGLPLFRLMQLNCSNPCDYNAYTHYAVQQSGELNYVSIAPYSKFIIFSKELFFYQEFTSFKFNRFFPLIHIKNITSLKCEKLEWILLWKTTNETIARGFWLLKIFKMSRITNISTNLPYHNIAYTFDFKFFCDLINIYNKILIVAFTINN 2796
            F+ +++L++   P+ I   S  +C  +     +  ECIF+ Q F +G+          + Y S                   K +C VGRENYQMEVFRKYFDLPIQVMPKISLIPPIPAIIQDLQCYYSDYFSLLVYNWGCYLSKGNKVDRTFILSLRQNENTLLLDYGSLNSIITCFCEIEFRFQKIIQIGPRAYFILPET                DKLKISPSNPTIFETLTCISRSKKLYKFNWYCNLDNFLVSINYPVNINYLSDYSKYLVRNIKIDSMNRMIFKLDFQLKNITCTCEVFYTSIYFS QLLNFPLPISDYPDLFIIPSVFAYGNIITCQTNKSRFFE+  +   QN             GE                  + I+  +DIYCECYFRFISLLSFSKK+AKFILPKSGLPLFRLMQLNCSNPCDYNAYTHYAVQQSGELNYVSIAPYSKFIIFSKELFFYQEFTSFKFNRFFPLIHIKNITSLKCEKLEWILLWKTTNETIARGFWLLKIFKMSRITNISTNLPYHNIAYTFDFKFFCDLINIYNKILIVAFTINN
Sbjct:   57 FVLAKDLIVG--PVTIFNDSAGSCGYAPQIIDVQVECIFHGQSFVIGQK--------TKTYVS-------------------KKKCTVGRENYQMEVFRKYFDLPIQVMPKISLIPPIPAIIQDLQCYYSDYFSLLVYNWGCYLSKGNKVDRTFILSLRQNENTLLLDYGSLNSIITCFCEIEFRFQKIIQIGPRAYFILPET----------------DKLKISPSNPTIFETLTCISRSKKLYKFNWYCNLDNFLVSINYPVNINYLSDYSKYLVRNIKIDSMNRMIFKLDFQLKNITCTCEVFYTSIYFSTQLLNFPLPISDYPDLFIIPSVFAYGNIITCQTNKSRFFEH--QATSQN------------WGE------------------NKIFLFIDIYCECYFRFISLLSFSKKSAKFILPKSGLPLFRLMQLNCSNPCDYNAYTHYAVQQSGELNYVSIAPYSKFIIFSKELFFYQEFTSFKFNRFFPLIHIKNITSLKCEKLEWILLWKTTNETIARGFWLLKIFKMSRITNISTNLPYHNIAYTFDFKFFCDLINIYNKILIVAFTINN 558          

HSP 2 Score: 220.32 bits (560), Expect = 1.216e-60
Identity = 107/107 (100.00%), Postives = 107/107 (100.00%), Query Frame = 1
Query:  292 MLNFEYKWIMTQQIYRVLVLLVLMTLKINVELEFSIYDMLFSCHAIINQQKYTARKFVLAKDLIVGPVTIFNDSAGSCGYAPQIIDVQVECIFHGQSFVIGQKTKTY 612
            MLNFEYKWIMTQQIYRVLVLLVLMTLKINVELEFSIYDMLFSCHAIINQQKYTARKFVLAKDLIVGPVTIFNDSAGSCGYAPQIIDVQVECIFHGQSFVIGQKTKTY
Sbjct:    1 MLNFEYKWIMTQQIYRVLVLLVLMTLKINVELEFSIYDMLFSCHAIINQQKYTARKFVLAKDLIVGPVTIFNDSAGSCGYAPQIIDVQVECIFHGQSFVIGQKTKTY 107          
BLAST of SMED30029559 vs. Planmine SMEST
Match: SMESG000072317.1 (SMESG000072317.1)

HSP 1 Score: 476.863 bits (1226), Expect = 8.002e-148
Identity = 274/771 (35.54%), Postives = 409/771 (53.05%), Query Frame = 1
Query:  373 INVELEFSIYDMLFSCHAIINQQKYTARKFVLAKDLIVGPV--TIFNDSAGSCGYAPQIIDVQVECIFHGQSFVIGQKTKTYGLNPTELYYFENIKHNRSEITFSHVFLNFTIKCLCSLTCFLNPFSLETYLKFQYPRKNASFLKIDPFPILLKTEITCSLEIQIPFESITWNCGFCQSFSNNYLSEICKSIKYNGNKIVINMDNIFAPNLSCLCVCIADEIYPPYMNLPMFIPSENLVIELYPLPINPYSNLTCNASLP--FPFLMWECIFNNQFFELGKNIIFS-NNNDKEMYYSLIVYQNKLLIYYFENNQSIKCRCIVGRENYQMEVFRKYFDLPIQVMPKISLIPPIPAIIQDLQCYYSDYFSLLVYNWGCYLSKGNKVDRTFILSLRQNENTLLLDYGSLNSIITCFCEIEFRFQKIIQIGPRAYFILPETGFLLYIFSYSN*IKNLDKLKISPSNPTIFETLTCISRSKKLYKFNWYCNLDNFLVSINYPVNINYLSDYSKYLVRNIKIDSMNRMIFKLDFQLKNITCTCEVFYTSIYFSNQLLNFPLPISDYPDLFIIPSVFAYGNIITCQTNKSRFFEYFWKCIYQNVTVYLGTKPPPRIGEKIKYFYSVFIVNKNSLLAHPIWTEVDIYCECYFRFISLLSFSKKTAKFILPKSGLPLFRLMQLNCSNPCDYNAYTHYAVQQSGELNYVSIAPYSKFIIFSKELFFYQEFTSFKFNRFFPLIHIKNITSLKCEKLEWILLWKTTNETIARGFWLLKIFKMS 2670
            +++EL FS   ++  C  ++  + +T    + +K +   P   T   ++ G C   P+ I+V+  C F      I +      L+P  +Y F +I+  R  ++F + FLNFT+ C C    FL PFS      F  P K  +FL++ P P+ L   I C       ++S  W C FC   +  Y   IC   K     I  ++D IF P+L C C+ + D  YP Y+++P+ +PS NL I +YPLP+  +  +TC A +P   PF  WEC FNN F  LGK+   S    D+ +  SL V   KL I    N + IKCRC VG + Y  + FRKYFD+P+ V    +L P  P I  +L C Y +      Y+WGC+LSK NK +R+FI S+R ++N L L+YG L     CFCEI F      Q GPR YF LP++                +++ I PS P + + + C  ++       W+C LD   V I      N LS YSKY+  ++ +++ ++ I  L  Q+  I C C+    S +F      F LPISDYPD +I P    +     C +  S+FF+Y +KC Y+N T+YL   P   I E +KYF     ++ N    + +W +++I CECY++F        KT  F LP S  PLF+ + +NCS PCDYN+ T YA++Q+ +LNY  IA + +F+I   +L+F+Q+F    FN FFPL +  ++ S KC KL+W++ W  T++ I++GFW LK++ + 
Sbjct:   76 LDIELVFSPQQLIIYCKVLVGSKYFTGSTILRSKKM---PFIYTELTNTTGQCSTVPKKIEVEFYCFFPAGLIAISKPNNR--LSPDMMYIFNSIQSTREFLSFKYEFLNFTLSCFCR--AFLEPFSASG--SFMLPFKLVNFLRVYPLPVSLNQWINCEYLYTKKYKSFEWVCDFCVGATEKYYIYICNYFKRKQKSINFDIDYIFLPSLDCKCLLLYDNFYPLYVDIPVRLPSSNLSIYVYPLPVKLFQLMTCYA-IPQVMPFFTWECKFNNTFVRLGKSSYPSLPEEDENVCRSLFVTGQKLYISLIYNIEIIKCRCAVGHDYYNFDEFRKYFDIPVIVSGVFTLTPAKPQINSNLLCTYDEMSDKYRYDWGCFLSKSNKTERSFIRSMRISDNELFLEYGELKWEYICFCEINFNIILTYQTGPRKYFNLPQS----------------ERIYIEPSKPMLKDVVNCRDQNSIYQTAKWFCILDGITVLIKDSSRYNDLSLYSKYIFNSLNVNNRSQFILNLSLQIMEIVCYCQTSSRSKFFPITNFYFKLPISDYPDPYIYPIPVKFNVGTKCTSFFSQFFKYLFKCQYKNYTLYLNQNPLFTIEEDLKYFLKSIYISNNVFYIYEMWLKINITCECYYKFKWGNDIINKTVSFKLPISTNPLFQSISINCSEPCDYNSKTRYALEQTSKLNYPLIASHYRFVIMCHDLYFFQQFKKSDFNVFFPLENDTDVISFKCVKLQWMMFWYNTDDVISQGFWSLKVYNVD 820          
The following BLAST results are available for this feature:
BLAST of SMED30029559 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30029559 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30029559 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30029559 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30029559 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30029559 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30029559 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30029559 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30029559 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30029559 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30029559 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30029559 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30029559 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30029559 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000072318.10.000e+090.07SMESG000072318.1[more]
SMESG000072318.11.671e-8478.44SMESG000072318.1[more]
SMESG000072318.18.613e-5378.69SMESG000072318.1[more]
SMESG000072318.11.216e-6077.20SMESG000072318.1[more]
SMESG000072317.18.002e-14835.54SMESG000072317.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30029559 ID=SMED30029559|Name=SMED30029559|organism=Schmidtea mediterranea sexual|type=transcript|length=3503bp
ATTTACATATTGTAAATATTTTGACGAACATTAAGCTTAATTTTCATTTA
AAAATCTCTTTTTCTTATAAAACCAATAATGTTATTTCAATGACAATAGC
TTTTTCAATTTTGCGGAAAGAATTTTATTTCGATTTTCCAGGTAAAAAAT
TGATTCAAACAGCCTCATCTATTGTTTACATTTTTTTATTGACAATAAAT
TTGACAAATTCAGCAATTAAATTGACTTTAGAAAATGTAGATTATCAAAG
TAGAGGTGAATGTGTTGTTCATCAATATAAGGGTAAAGTTGATGTTAAAT
TTCGAATACAAATGGATAATGACGCAACAGATTTACCGAGTTCTGGTGTT
ATTGGTTTTGATGACTTTAAAAATAAATGTTGAACTTGAATTTTCTATTT
ATGATATGCTATTTTCCTGCCATGCTATTATCAATCAACAAAAGTATACA
GCAAGAAAATTTGTATTAGCAAAAGACTTGATAGTTGGTCCAGTGACCAT
ATTTAATGATAGTGCTGGTTCATGTGGTTATGCTCCGCAAATCATTGACG
TTCAAGTTGAATGCATATTTCATGGTCAATCATTTGTAATTGGTCAAAAG
ACAAAGACATATGGTTTAAATCCAACCGAACTATATTACTTTGAAAATAT
AAAACATAATAGATCTGAAATCACATTTTCTCATGTTTTCCTCAATTTCA
CTATTAAATGCTTGTGCTCACTCACATGTTTTCTTAATCCATTCTCACTT
GAAACATATTTGAAATTCCAGTATCCAAGAAAAAATGCCTCATTTTTAAA
AATTGATCCTTTTCCAATTCTATTAAAAACCGAAATAACTTGTTCATTAG
AAATTCAAATACCATTTGAATCAATTACATGGAATTGTGGATTCTGCCAG
AGCTTTTCTAACAATTACTTATCTGAAATATGCAAATCAATAAAATATAA
CGGCAATAAAATTGTTATAAATATGGATAACATTTTCGCACCCAATCTTT
CATGTTTATGCGTTTGTATTGCCGATGAAATTTATCCTCCTTACATGAAT
TTACCCATGTTTATTCCATCAGAAAATCTTGTCATTGAATTATATCCATT
GCCGATAAATCCGTATTCCAATCTCACTTGTAATGCATCACTTCCTTTTC
CATTTTTAATGTGGGAATGCATCTTCAATAACCAATTTTTTGAACTTGGT
AAAAATATCATATTTTCAAATAATAATGACAAAGAAATGTATTATTCTTT
GATAGTGTATCAAAATAAGCTTTTAATTTATTATTTTGAAAATAATCAGT
CGATTAAATGCAGATGTATAGTTGGACGGGAAAATTATCAAATGGAAGTT
TTCAGAAAATATTTTGATTTGCCAATTCAAGTGATGCCGAAAATTAGTTT
AATTCCACCAATACCAGCAATAATTCAAGATTTACAATGCTATTATTCTG
ATTACTTTTCTTTATTGGTATATAATTGGGGTTGTTACTTATCTAAAGGT
AATAAAGTTGACAGAACTTTCATTTTAAGCTTACGTCAAAATGAGAATAC
GTTACTTTTGGATTATGGAAGTTTGAATTCTATAATAACTTGTTTTTGTG
AAATTGAATTTCGATTTCAAAAAATAATTCAAATCGGACCAAGAGCATAT
TTTATATTACCCGAAACCGGTTTTCTTTTGTATATTTTTTCATATTCAAA
TTAAATCAAAAATTTAGACAAACTGAAAATATCTCCCAGTAATCCCACTA
TTTTCGAAACTTTAACTTGCATATCCAGATCAAAAAAACTATATAAATTC
AACTGGTATTGCAATCTTGACAATTTCTTGGTTTCAATTAATTATCCAGT
TAATATAAATTATTTATCAGATTACTCGAAATATTTAGTAAGAAATATAA
AGATTGACTCTATGAACAGAATGATTTTCAAACTGGATTTCCAATTGAAA
AACATTACTTGCACATGTGAAGTATTTTATACATCTATTTACTTTTCAAA
TCAACTACTGAATTTTCCTTTGCCAATTTCAGATTATCCAGATTTGTTCA
TTATTCCTAGTGTATTCGCTTATGGCAATATCATTACATGCCAAACAAAT
AAATCGCGTTTTTTCGAGTATTTCTGGAAATGTATTTATCAGAACGTAAC
GGTTTATTTAGGCACCAAGCCACCTCCCAGAATTGGGGAGAAAATAAAAT
ATTTCTATTCAGTTTTTATAGTCAATAAAAACTCACTACTTGCTCATCCA
ATTTGGACTGAAGTTGATATTTATTGTGAATGCTATTTCAGATTTATTAG
TTTATTATCGTTTTCCAAAAAAACTGCAAAATTCATTTTACCTAAATCAG
GTTTACCATTATTTCGTCTTATGCAACTCAATTGTTCCAACCCATGTGAT
TATAATGCTTATACACATTATGCCGTTCAACAATCCGGAGAATTAAATTA
TGTATCTATTGCTCCTTATTCCAAATTCATTATATTCTCCAAGGAATTAT
TCTTTTACCAAGAATTCACTAGCTTTAAATTTAATAGGTTTTTCCCACTT
ATCCATATTAAGAACATTACGTCTTTGAAATGTGAAAAATTGGAATGGAT
TTTATTGTGGAAAACAACTAACGAAACTATTGCTAGAGGATTTTGGTTGT
TGAAAATATTTAAAATGTCTCGCATTACTAATATATCAACCAATCTCCCA
TATCACAATATAGCTTATACATTTGACTTTAAATTTTTCTGTGATTTGAT
TAACATTTACAATAAAATACTTATAGTAGCTTTCACTATTAATAATGGTA
TGCTAATGGACTTTTTTTATGTTATTAATAATACAATAATGATTAATACT
CTCATTCCAAAAAAAGATTCCATTTTAATTACTTGCCATATATATATTTA
TGATCAGAAGTTTCCTGTTTTATGCCGAATGTATAGAATTTCTAATTTAT
CTAAAATATTAGTCTTTCAATCTTATAAAAATATCAATGTATCCATTTAC
AAATTACATGTTTATAATTATCCATTTGCTGTAGTAATTGAAATGTGTTG
GCCTATAACTGTAGAAATCATTCCTATGTTGCCTGATGCTAATCCTAAAC
TTTCTTTATGGGCTCACTACGAAGTTGACGAACGAATTCCGTCTAAAACT
TTTTTTTACCACATTGTTATTTCTGAAATCGATGGATTTTATCAAATTTA
TTATACATATATATCAAGTGATTCGATTTTCAGAAAGAATGAAATATTAA
ATTATGGAATGCGCAGTAATACGTTTTACATCATAACAAAGATTTCACTC
GAAATTACAAAATTAGCCCAAATATTAATTGTTTTATAACCAATCTATTT
GTAAAATAGTTAATTGATTGCTAGTCTAACTTAAACTTAATCATAATCTG
TGGGACGGGCAAACATACAACTCCTATTATAAAATGAAAACTTGAATAAC
TTTATATTTTATAGACATACATGCTTACCTGTGTGGTAATGTAATTTGTT
CGA
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protein sequence of SMED30029559-orf-1

>SMED30029559-orf-1 ID=SMED30029559-orf-1|Name=SMED30029559-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=127bp
LHIVNILTNIKLNFHLKISFSYKTNNVISMTIAFSILRKEFYFDFPGKKL
IQTASSIVYIFLLTINLTNSAIKLTLENVDYQSRGECVVHQYKGKVDVKF
RIQMDNDATDLPSSGVIGFDDFKNKC*
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protein sequence of SMED30029559-orf-2

>SMED30029559-orf-2 ID=SMED30029559-orf-2|Name=SMED30029559-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=471bp
MLNFEYKWIMTQQIYRVLVLLVLMTLKINVELEFSIYDMLFSCHAIINQQ
KYTARKFVLAKDLIVGPVTIFNDSAGSCGYAPQIIDVQVECIFHGQSFVI
GQKTKTYGLNPTELYYFENIKHNRSEITFSHVFLNFTIKCLCSLTCFLNP
FSLETYLKFQYPRKNASFLKIDPFPILLKTEITCSLEIQIPFESITWNCG
FCQSFSNNYLSEICKSIKYNGNKIVINMDNIFAPNLSCLCVCIADEIYPP
YMNLPMFIPSENLVIELYPLPINPYSNLTCNASLPFPFLMWECIFNNQFF
ELGKNIIFSNNNDKEMYYSLIVYQNKLLIYYFENNQSIKCRCIVGRENYQ
MEVFRKYFDLPIQVMPKISLIPPIPAIIQDLQCYYSDYFSLLVYNWGCYL
SKGNKVDRTFILSLRQNENTLLLDYGSLNSIITCFCEIEFRFQKIIQIGP
RAYFILPETGFLLYIFSYSN*
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protein sequence of SMED30029559-orf-3

>SMED30029559-orf-3 ID=SMED30029559-orf-3|Name=SMED30029559-orf-3|organism=Schmidtea mediterranea sexual|type=polypeptide|length=476bp
MNRMIFKLDFQLKNITCTCEVFYTSIYFSNQLLNFPLPISDYPDLFIIPS
VFAYGNIITCQTNKSRFFEYFWKCIYQNVTVYLGTKPPPRIGEKIKYFYS
VFIVNKNSLLAHPIWTEVDIYCECYFRFISLLSFSKKTAKFILPKSGLPL
FRLMQLNCSNPCDYNAYTHYAVQQSGELNYVSIAPYSKFIIFSKELFFYQ
EFTSFKFNRFFPLIHIKNITSLKCEKLEWILLWKTTNETIARGFWLLKIF
KMSRITNISTNLPYHNIAYTFDFKFFCDLINIYNKILIVAFTINNGMLMD
FFYVINNTIMINTLIPKKDSILITCHIYIYDQKFPVLCRMYRISNLSKIL
VFQSYKNINVSIYKLHVYNYPFAVVIEMCWPITVEIIPMLPDANPKLSLW
AHYEVDERIPSKTFFYHIVISEIDGFYQIYYTYISSDSIFRKNEILNYGM
RSNTFYIITKISLEITKLAQILIVL*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000418head