SMED30029516

Overview
NameSMED30029516
Smed IDSMED30029516
Length (bp)517
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30029516 (SMED30029516) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30029516 (SMED30029516) in cells (dots) of each of the 12 neoblast clusters.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30029516

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
parenchymal cellSMED30029516SMESG000019002.1 dd_Smed_v4_11134_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30029516SMESG000019002.1 dd_Smed_v4_11134_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of SMED30029516 vs. Ensembl Human
Match: ZBED9 (zinc finger BED-type containing 9 [Source:HGNC Symbol;Acc:HGNC:13851])

HSP 1 Score: 58.9214 bits (141), Expect = 4.484e-10
Identity = 28/67 (41.79%), Postives = 44/67 (65.67%), Query Frame = -1
Query:  290 NSLNSRIFGLLSDAINSDFKTLLHHTEVRLLFKGKVLERVIHLKTKIISFLEVEDITLNFDFRDSDW 490
            NSLNSR+F LL D + +D K LL H E+R L +GKVL R+  ++ +++ FL+ +    +  F+D +W
Sbjct: 1032 NSLNSRLFSLLCDNMEADHKQLLLHAEIRWLSRGKVLSRMFEIRNELLVFLQGKKPMWSQLFKDVNW 1098          
BLAST of SMED30029516 vs. Ensembl Human
Match: ZBED9 (zinc finger BED-type containing 9 [Source:HGNC Symbol;Acc:HGNC:13851])

HSP 1 Score: 57.3806 bits (137), Expect = 1.623e-9
Identity = 28/67 (41.79%), Postives = 44/67 (65.67%), Query Frame = -1
Query:  290 NSLNSRIFGLLSDAINSDFKTLLHHTEVRLLFKGKVLERVIHLKTKIISFLEVEDITLNFDFRDSDW 490
            NSLNSR+F LL D + +D K LL H E+R L +GKVL R+  ++ +++ FL+ +    +  F+D +W
Sbjct:  881 NSLNSRLFSLLCDNMEADHKQLLLHAEIRWLSRGKVLSRMFEIRNELLVFLQGKKPMWSQLFKDVNW 947          
BLAST of SMED30029516 vs. Ensembl Human
Match: ZMYM6 (zinc finger MYM-type containing 6 [Source:HGNC Symbol;Acc:HGNC:13050])

HSP 1 Score: 46.595 bits (109), Expect = 8.244e-6
Identity = 23/67 (34.33%), Postives = 40/67 (59.70%), Query Frame = -1
Query:  290 NSLNSRIFGLLSDAINSDFKTLLHHTEVRLLFKGKVLERVIHLKTKIISFLEVEDITLNFDFRDSDW 490
            N+LNSR+  +L + + S+  +L  H EVR + +G++L+R+  L+ +I  FL  +   L   F D +W
Sbjct: 1043 NALNSRMLTILCEEMGSEHVSLPLHAEVRWISRGRMLKRLFELRHEIEIFLSQKHSDLAKYFHDEEW 1109          
BLAST of SMED30029516 vs. UniProt/SwissProt
Match: sp|Q6R2W3|SCND3_HUMAN (SCAN domain-containing protein 3 OS=Homo sapiens OX=9606 GN=ZBED9 PE=2 SV=1)

HSP 1 Score: 58.9214 bits (141), Expect = 2.153e-9
Identity = 28/67 (41.79%), Postives = 44/67 (65.67%), Query Frame = -1
Query:  290 NSLNSRIFGLLSDAINSDFKTLLHHTEVRLLFKGKVLERVIHLKTKIISFLEVEDITLNFDFRDSDW 490
            NSLNSR+F LL D + +D K LL H E+R L +GKVL R+  ++ +++ FL+ +    +  F+D +W
Sbjct: 1032 NSLNSRLFSLLCDNMEADHKQLLLHAEIRWLSRGKVLSRMFEIRNELLVFLQGKKPMWSQLFKDVNW 1098          
BLAST of SMED30029516 vs. TrEMBL
Match: A0A1R1Y9U3 (SCAN domain-containing protein 3 OS=Smittium culicis OX=133412 GN=AYI70_g2078 PE=4 SV=1)

HSP 1 Score: 77.7962 bits (190), Expect = 3.142e-15
Identity = 38/68 (55.88%), Postives = 51/68 (75.00%), Query Frame = -1
Query:  284 SLNSRIFGLLSDAINSDFKTLLHHTEVRLLFKGKVLERVIHLKTKIISFLEVEDITLNFDFRDSDWWL 487
             LNSRIF  L DA++SD+K LL++T+VR L KGK L+R++HLK ++ISFL+VE+  L F   D  WWL
Sbjct:   58 PLNSRIFAQLCDAMDSDYKFLLYNTKVRWLSKGKFLKRLVHLKVEVISFLKVEEADLAFYDHDELWWL 125          
BLAST of SMED30029516 vs. TrEMBL
Match: A0A0L8HJ94 (Uncharacterized protein (Fragment) OS=Octopus bimaculoides OX=37653 GN=OCBIM_22013539mg PE=4 SV=1)

HSP 1 Score: 61.6178 bits (148), Expect = 2.210e-9
Identity = 35/75 (46.67%), Postives = 45/75 (60.00%), Query Frame = -1
Query:  266 NSLNSRIFGLLSDAINSDFKTLLHHTEVRLLFKGKVLERVIHLKTKIISFLEVEDITLNFDFRDSDWWLNGSAEN 490
            +SL  R F  L + ++SDFK LL HTEVR L KGKV  R I L+T+I  F +VE+    F   D +WWL  +  N
Sbjct:   12 HSLQCRRFVSLGEIMDSDFKCLLLHTEVRWLTKGKVFTRFISLRTEIKCFFDVENSGFEF-LNDDNWWLEAAFLN 85          
BLAST of SMED30029516 vs. TrEMBL
Match: A0A2H1CX04 (Uncharacterized protein OS=Fasciola hepatica OX=6192 GN=D915_01027 PE=4 SV=1)

HSP 1 Score: 60.8474 bits (146), Expect = 1.631e-8
Identity = 33/73 (45.21%), Postives = 48/73 (65.75%), Query Frame = -1
Query:  275 NSLNSRIFGLLSDAINSDFKTLLHHTEVRLLFKGKVLERVIHLKTKIISFLEVEDITLNFDFRDSD-WWLNGS 490
            N L +RI   L +A+ SD+K  L HT+ R L +GK L+R+I L+ ++IS+ E ED T +F+F  +D WWL  S
Sbjct:   33 NPLRTRISASLCEAMESDYKGSLLHTDTRFLSEGKALDRIILLRIEVISYFETED-TNDFEFLHNDVWWLPVS 104          
BLAST of SMED30029516 vs. TrEMBL
Match: A0A2S2PLL0 (Zinc finger BED domain-containing protein 5 (Fragment) OS=Schizaphis graminum OX=13262 GN=ZBED5_0 PE=4 SV=1)

HSP 1 Score: 63.5438 bits (153), Expect = 1.645e-8
Identity = 42/124 (33.87%), Postives = 64/124 (51.61%), Query Frame = -1
Query:  185 QLNSLNSRIFGLLSDAINSDFKTLLHHTEVRLLFKGKVLERVIHLKTKIISFLEVEDITLNFDFRDSDWWL--------------------NGSAENIMTATSKLLSFKVKLVHQKNQGKKLRF 496
            ++  L +R+F LL +A+ S F  L+ HTEVR L +GKVL RV  LK ++I F  +E++    +   ++ WL                     G  ENI+T+ SKL + K KL   K + +K +F
Sbjct:  235 KMRPLKTRLFSLLCEAMESQFTKLILHTEVRWLSRGKVLSRVHELKNELIVFFTLENVPEFCELLTNEKWLEKLSYLADIFSHLNQINSSMQGPNENILTSCSKLFTLKDKLKIWKKRVQKNQF 358          
BLAST of SMED30029516 vs. TrEMBL
Match: A0A2S2NJ19 (Zinc finger BED domain-containing protein 5 (Fragment) OS=Schizaphis graminum OX=13262 GN=ZBED5_11 PE=4 SV=1)

HSP 1 Score: 63.1586 bits (152), Expect = 2.015e-8
Identity = 43/126 (34.13%), Postives = 61/126 (48.41%), Query Frame = -1
Query:  176 NSLNSRIFGLLSDAINSDFKTLLHHTEVRLLFKGKVLERVIHLKTKIISFLEVEDITLNFDFRDSDWW-------------------LNGSAENIMTATSKLLSFKVKLVHQKNQ--GKKLRFSPH 490
            +SL SR+F  + D ++SD   LL H+ +R L +G+VL R   L  +II FL +E+    F   D  WW                   + G  ENI+T+T K+++F  KL   K Q   KKL   P 
Sbjct:  324 SSLKSRLFEKICDGMDSDNSKLLFHSAIRWLSRGRVLSRFYDLSEEIIVFLTMEESKYEF-LGDEKWWTKVSFLTDIFEHLNKLNTSMQGRNENILTSTDKMMAFNEKLTLWKTQVDQKKLTMFPR 448          
BLAST of SMED30029516 vs. Ensembl Medaka
Match: ENSORLT00000032459.1 (pep primary_assembly:ASM223467v1:8:14240678:14247355:1 gene:ENSORLG00000024991.1 transcript:ENSORLT00000032459.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 50.0618 bits (118), Expect = 2.851e-7
Identity = 23/51 (45.10%), Postives = 34/51 (66.67%), Query Frame = -1
Query:  338 NSLNSRIFGLLSDAINSDFKTLLHHTEVRLLFKGKVLERVIHLKTKIISFL 490
            N++NSR F  L + + +D   LL+H+EVR L +G VL R+  L+ +I SFL
Sbjct:  308 NAVNSRCFAQLCEGMEADHVQLLYHSEVRWLSRGLVLTRIFELRNEIFSFL 358          
BLAST of SMED30029516 vs. Ensembl Medaka
Match: ENSORLT00000030244.1 (pep primary_assembly:ASM223467v1:13:28104458:28106771:-1 gene:ENSORLG00000022768.1 transcript:ENSORLT00000030244.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 48.9062 bits (115), Expect = 5.586e-7
Identity = 22/51 (43.14%), Postives = 34/51 (66.67%), Query Frame = -1
Query:  338 NSLNSRIFGLLSDAINSDFKTLLHHTEVRLLFKGKVLERVIHLKTKIISFL 490
            N++NSR F  L + + +D   LL+H+EVR L +G VL R+  L+ ++ SFL
Sbjct:  179 NAVNSRCFAQLCEGMEADHVKLLYHSEVRWLSRGLVLTRIFELRKEVFSFL 229          
BLAST of SMED30029516 vs. Ensembl Medaka
Match: ENSORLT00000028620.1 (pep primary_assembly:ASM223467v1:3:35179252:35181570:1 gene:ENSORLG00000028714.1 transcript:ENSORLT00000028620.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 47.7506 bits (112), Expect = 1.690e-6
Identity = 21/51 (41.18%), Postives = 33/51 (64.71%), Query Frame = -1
Query:  338 NSLNSRIFGLLSDAINSDFKTLLHHTEVRLLFKGKVLERVIHLKTKIISFL 490
            N++NSR F  L + + +D   LL+H+EVR L +G V  R+  L+ ++ SFL
Sbjct:  355 NAVNSRCFAQLCEGMEADHVKLLYHSEVRWLSRGLVFTRIFELRKEVFSFL 405          
BLAST of SMED30029516 vs. Planmine SMEST
Match: SMESG000036914.1 (SMESG000036914.1)

HSP 1 Score: 60.077 bits (144), Expect = 5.310e-11
Identity = 39/91 (42.86%), Postives = 56/91 (61.54%), Query Frame = -1
Query:  221 NSLNSRIFGLLSDAINSDFKTLLHHTEVRLLFKGKVLERVIHLKTKIISFLEVEDITLNFD-FRDSDWWLNGSAENIMTATSKLLSFKVKL 490
            N+L SRIF  L +A++SD+KTLL+HT V+   KGK+L R ++ K   I +L V  +   F+ F D +  L G+  N +  TSKL  F+ KL
Sbjct:  153 NALQSRIFAALCEAMDSDYKTLLYHTVVQWHSKGKILNRFVYEKD--ICWLPVSFLNDFFEKFNDLNLKLQGAQGNFINITSKLKGFEEKL 241          
BLAST of SMED30029516 vs. Planmine SMEST
Match: SMESG000028317.1 (SMESG000028317.1)

HSP 1 Score: 46.9802 bits (110), Expect = 2.093e-6
Identity = 34/111 (30.63%), Postives = 56/111 (50.45%), Query Frame = -1
Query:  209 RIFGLLSDAINSDFKTLLHHTEVRLLFKGKVLERVIHLKTKIISFLEVEDITLNFDFRDSDWW---------------LNGS-----AENIMTATSKLLSF--KVKLVHQK 475
            R+F  + +  NS F+ L++H E+R L KGK L R+  L  ++ +FL  E+ ++   F+++ W                LN S      + I+ A  K+LSF  K+ L+ QK
Sbjct:  934 RLFARVYENSNSQFEILINHAEMRWLSKGKSLRRLFDLSDEVRTFLMAENPSMTDFFQNAQWLCTFAYLADIFEKLNDLNKSLQGKGCDKILFAYDKILSFVKKLALLQQK 1044          
BLAST of SMED30029516 vs. Planmine SMEST
Match: SMESG000037770.1 (SMESG000037770.1)

HSP 1 Score: 46.595 bits (109), Expect = 3.491e-6
Identity = 26/55 (47.27%), Postives = 38/55 (69.09%), Query Frame = -1
Query:  290 INSDFKTLLHHTEVRLLFKGKVLERVIHLKTKIISFLEVEDITLNF--DFRDSDW 448
            + SD   +L+HT VR L  GKVL+RV +LK +I+ FLE++DIT+ F    + S+W
Sbjct:  832 LESDHSDVLYHTNVRWLSLGKVLKRVWNLKDEIVLFLEMKDITVEFTTQMKTSEW 886          
BLAST of SMED30029516 vs. Planmine SMEST
Match: SMESG000024836.1 (SMESG000024836.1)

HSP 1 Score: 44.669 bits (104), Expect = 6.289e-6
Identity = 24/54 (44.44%), Postives = 34/54 (62.96%), Query Frame = -1
Query:  326 SLNSRIFGLLSDAINSDFKTLLHHTEVRLLFKGKVLERVIHLKTKIISFLEVED 487
             LN R F  L + + SD++ L ++TE+R L   KVL R + L  KI+ FLEV+D
Sbjct:   57 GLNHREFTTLLNDVESDYEDLPYYTEIRWLPSHKVLRRFLQLFDKIVLFLEVKD 110          
The following BLAST results are available for this feature:
BLAST of SMED30029516 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 3
Match NameE-valueIdentityDescription
ZBED94.484e-1041.79zinc finger BED-type containing 9 [Source:HGNC Sym... [more]
ZBED91.623e-941.79zinc finger BED-type containing 9 [Source:HGNC Sym... [more]
ZMYM68.244e-634.33zinc finger MYM-type containing 6 [Source:HGNC Sym... [more]
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BLAST of SMED30029516 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30029516 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30029516 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30029516 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30029516 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30029516 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 1
Match NameE-valueIdentityDescription
sp|Q6R2W3|SCND3_HUMAN2.153e-941.79SCAN domain-containing protein 3 OS=Homo sapiens O... [more]
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BLAST of SMED30029516 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1R1Y9U33.142e-1555.88SCAN domain-containing protein 3 OS=Smittium culic... [more]
A0A0L8HJ942.210e-946.67Uncharacterized protein (Fragment) OS=Octopus bima... [more]
A0A2H1CX041.631e-845.21Uncharacterized protein OS=Fasciola hepatica OX=61... [more]
A0A2S2PLL01.645e-833.87Zinc finger BED domain-containing protein 5 (Fragm... [more]
A0A2S2NJ192.015e-834.13Zinc finger BED domain-containing protein 5 (Fragm... [more]
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BLAST of SMED30029516 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30029516 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30029516 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30029516 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30029516 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 3
Match NameE-valueIdentityDescription
ENSORLT00000032459.12.851e-745.10pep primary_assembly:ASM223467v1:8:14240678:142473... [more]
ENSORLT00000030244.15.586e-743.14pep primary_assembly:ASM223467v1:13:28104458:28106... [more]
ENSORLT00000028620.11.690e-641.18pep primary_assembly:ASM223467v1:3:35179252:351815... [more]
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BLAST of SMED30029516 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 4
Match NameE-valueIdentityDescription
SMESG000036914.15.310e-1142.86SMESG000036914.1[more]
SMESG000028317.12.093e-630.63SMESG000028317.1[more]
SMESG000037770.13.491e-647.27SMESG000037770.1[more]
SMESG000024836.16.289e-644.44SMESG000024836.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30029516 ID=SMED30029516|Name=SMED30029516|organism=Schmidtea mediterranea sexual|type=transcript|length=517bp
TTCTGCTTCTTTCGTGGAAAATTTAAGACATTCGTGTGTTCCAGATGTAT
TCAAAATTCACTGATAAAAGTTGACGTTTAAGGAGAGAAAATAATTGTCG
AGGGCTTTTAAAAGCATTTACATGGTTTGGAAAGCAAGATCAGAAATTTC
ATCTTTATCAGTGAATTTGCCAACGATGGGGAGAAAATCGAAGCTTTTTT
CCTTGATTTTTCTGATGAACAAGTTTTACTTTGAATGAAAGAAGCTTTGA
AGTTGCCGTCATAATATTTTCAGCTGATCCATTCAACCACCAGTCACTGT
CTCGAAAATCAAAATTGAGTGTGATATCTTCAACTTCAAGAAATGAAATT
ATTTTAGTTTTGAGGTGTATAACACGCTCAAGAACTTTTCCTTTAAACAG
CAATCGAACTTCTGTGTGGTGGAGCAGAGTTTTGAAATCAGAATTTATCG
CATCGCTAAGAAGGCCAAAAATTCTGGAGTTCAAAGAATTTAACTGATTT
ATTCAACAAATATTTAT
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000101muscle cell
PLANA:0003116parenchymal cell