SMED30029516
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30029516 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 2
Homology
BLAST of SMED30029516 vs. Ensembl Human
Match: ZBED9 (zinc finger BED-type containing 9 [Source:HGNC Symbol;Acc:HGNC:13851]) HSP 1 Score: 58.9214 bits (141), Expect = 4.484e-10 Identity = 28/67 (41.79%), Postives = 44/67 (65.67%), Query Frame = -1 Query: 290 NSLNSRIFGLLSDAINSDFKTLLHHTEVRLLFKGKVLERVIHLKTKIISFLEVEDITLNFDFRDSDW 490 NSLNSR+F LL D + +D K LL H E+R L +GKVL R+ ++ +++ FL+ + + F+D +W Sbjct: 1032 NSLNSRLFSLLCDNMEADHKQLLLHAEIRWLSRGKVLSRMFEIRNELLVFLQGKKPMWSQLFKDVNW 1098
BLAST of SMED30029516 vs. Ensembl Human
Match: ZBED9 (zinc finger BED-type containing 9 [Source:HGNC Symbol;Acc:HGNC:13851]) HSP 1 Score: 57.3806 bits (137), Expect = 1.623e-9 Identity = 28/67 (41.79%), Postives = 44/67 (65.67%), Query Frame = -1 Query: 290 NSLNSRIFGLLSDAINSDFKTLLHHTEVRLLFKGKVLERVIHLKTKIISFLEVEDITLNFDFRDSDW 490 NSLNSR+F LL D + +D K LL H E+R L +GKVL R+ ++ +++ FL+ + + F+D +W Sbjct: 881 NSLNSRLFSLLCDNMEADHKQLLLHAEIRWLSRGKVLSRMFEIRNELLVFLQGKKPMWSQLFKDVNW 947
BLAST of SMED30029516 vs. Ensembl Human
Match: ZMYM6 (zinc finger MYM-type containing 6 [Source:HGNC Symbol;Acc:HGNC:13050]) HSP 1 Score: 46.595 bits (109), Expect = 8.244e-6 Identity = 23/67 (34.33%), Postives = 40/67 (59.70%), Query Frame = -1 Query: 290 NSLNSRIFGLLSDAINSDFKTLLHHTEVRLLFKGKVLERVIHLKTKIISFLEVEDITLNFDFRDSDW 490 N+LNSR+ +L + + S+ +L H EVR + +G++L+R+ L+ +I FL + L F D +W Sbjct: 1043 NALNSRMLTILCEEMGSEHVSLPLHAEVRWISRGRMLKRLFELRHEIEIFLSQKHSDLAKYFHDEEW 1109
BLAST of SMED30029516 vs. UniProt/SwissProt
Match: sp|Q6R2W3|SCND3_HUMAN (SCAN domain-containing protein 3 OS=Homo sapiens OX=9606 GN=ZBED9 PE=2 SV=1) HSP 1 Score: 58.9214 bits (141), Expect = 2.153e-9 Identity = 28/67 (41.79%), Postives = 44/67 (65.67%), Query Frame = -1 Query: 290 NSLNSRIFGLLSDAINSDFKTLLHHTEVRLLFKGKVLERVIHLKTKIISFLEVEDITLNFDFRDSDW 490 NSLNSR+F LL D + +D K LL H E+R L +GKVL R+ ++ +++ FL+ + + F+D +W Sbjct: 1032 NSLNSRLFSLLCDNMEADHKQLLLHAEIRWLSRGKVLSRMFEIRNELLVFLQGKKPMWSQLFKDVNW 1098
BLAST of SMED30029516 vs. TrEMBL
Match: A0A1R1Y9U3 (SCAN domain-containing protein 3 OS=Smittium culicis OX=133412 GN=AYI70_g2078 PE=4 SV=1) HSP 1 Score: 77.7962 bits (190), Expect = 3.142e-15 Identity = 38/68 (55.88%), Postives = 51/68 (75.00%), Query Frame = -1 Query: 284 SLNSRIFGLLSDAINSDFKTLLHHTEVRLLFKGKVLERVIHLKTKIISFLEVEDITLNFDFRDSDWWL 487 LNSRIF L DA++SD+K LL++T+VR L KGK L+R++HLK ++ISFL+VE+ L F D WWL Sbjct: 58 PLNSRIFAQLCDAMDSDYKFLLYNTKVRWLSKGKFLKRLVHLKVEVISFLKVEEADLAFYDHDELWWL 125
BLAST of SMED30029516 vs. TrEMBL
Match: A0A0L8HJ94 (Uncharacterized protein (Fragment) OS=Octopus bimaculoides OX=37653 GN=OCBIM_22013539mg PE=4 SV=1) HSP 1 Score: 61.6178 bits (148), Expect = 2.210e-9 Identity = 35/75 (46.67%), Postives = 45/75 (60.00%), Query Frame = -1 Query: 266 NSLNSRIFGLLSDAINSDFKTLLHHTEVRLLFKGKVLERVIHLKTKIISFLEVEDITLNFDFRDSDWWLNGSAEN 490 +SL R F L + ++SDFK LL HTEVR L KGKV R I L+T+I F +VE+ F D +WWL + N Sbjct: 12 HSLQCRRFVSLGEIMDSDFKCLLLHTEVRWLTKGKVFTRFISLRTEIKCFFDVENSGFEF-LNDDNWWLEAAFLN 85
BLAST of SMED30029516 vs. TrEMBL
Match: A0A2H1CX04 (Uncharacterized protein OS=Fasciola hepatica OX=6192 GN=D915_01027 PE=4 SV=1) HSP 1 Score: 60.8474 bits (146), Expect = 1.631e-8 Identity = 33/73 (45.21%), Postives = 48/73 (65.75%), Query Frame = -1 Query: 275 NSLNSRIFGLLSDAINSDFKTLLHHTEVRLLFKGKVLERVIHLKTKIISFLEVEDITLNFDFRDSD-WWLNGS 490 N L +RI L +A+ SD+K L HT+ R L +GK L+R+I L+ ++IS+ E ED T +F+F +D WWL S Sbjct: 33 NPLRTRISASLCEAMESDYKGSLLHTDTRFLSEGKALDRIILLRIEVISYFETED-TNDFEFLHNDVWWLPVS 104
BLAST of SMED30029516 vs. TrEMBL
Match: A0A2S2PLL0 (Zinc finger BED domain-containing protein 5 (Fragment) OS=Schizaphis graminum OX=13262 GN=ZBED5_0 PE=4 SV=1) HSP 1 Score: 63.5438 bits (153), Expect = 1.645e-8 Identity = 42/124 (33.87%), Postives = 64/124 (51.61%), Query Frame = -1 Query: 185 QLNSLNSRIFGLLSDAINSDFKTLLHHTEVRLLFKGKVLERVIHLKTKIISFLEVEDITLNFDFRDSDWWL--------------------NGSAENIMTATSKLLSFKVKLVHQKNQGKKLRF 496 ++ L +R+F LL +A+ S F L+ HTEVR L +GKVL RV LK ++I F +E++ + ++ WL G ENI+T+ SKL + K KL K + +K +F Sbjct: 235 KMRPLKTRLFSLLCEAMESQFTKLILHTEVRWLSRGKVLSRVHELKNELIVFFTLENVPEFCELLTNEKWLEKLSYLADIFSHLNQINSSMQGPNENILTSCSKLFTLKDKLKIWKKRVQKNQF 358
BLAST of SMED30029516 vs. TrEMBL
Match: A0A2S2NJ19 (Zinc finger BED domain-containing protein 5 (Fragment) OS=Schizaphis graminum OX=13262 GN=ZBED5_11 PE=4 SV=1) HSP 1 Score: 63.1586 bits (152), Expect = 2.015e-8 Identity = 43/126 (34.13%), Postives = 61/126 (48.41%), Query Frame = -1 Query: 176 NSLNSRIFGLLSDAINSDFKTLLHHTEVRLLFKGKVLERVIHLKTKIISFLEVEDITLNFDFRDSDWW-------------------LNGSAENIMTATSKLLSFKVKLVHQKNQ--GKKLRFSPH 490 +SL SR+F + D ++SD LL H+ +R L +G+VL R L +II FL +E+ F D WW + G ENI+T+T K+++F KL K Q KKL P Sbjct: 324 SSLKSRLFEKICDGMDSDNSKLLFHSAIRWLSRGRVLSRFYDLSEEIIVFLTMEESKYEF-LGDEKWWTKVSFLTDIFEHLNKLNTSMQGRNENILTSTDKMMAFNEKLTLWKTQVDQKKLTMFPR 448
BLAST of SMED30029516 vs. Ensembl Medaka
Match: ENSORLT00000032459.1 (pep primary_assembly:ASM223467v1:8:14240678:14247355:1 gene:ENSORLG00000024991.1 transcript:ENSORLT00000032459.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 50.0618 bits (118), Expect = 2.851e-7 Identity = 23/51 (45.10%), Postives = 34/51 (66.67%), Query Frame = -1 Query: 338 NSLNSRIFGLLSDAINSDFKTLLHHTEVRLLFKGKVLERVIHLKTKIISFL 490 N++NSR F L + + +D LL+H+EVR L +G VL R+ L+ +I SFL Sbjct: 308 NAVNSRCFAQLCEGMEADHVQLLYHSEVRWLSRGLVLTRIFELRNEIFSFL 358
BLAST of SMED30029516 vs. Ensembl Medaka
Match: ENSORLT00000030244.1 (pep primary_assembly:ASM223467v1:13:28104458:28106771:-1 gene:ENSORLG00000022768.1 transcript:ENSORLT00000030244.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 48.9062 bits (115), Expect = 5.586e-7 Identity = 22/51 (43.14%), Postives = 34/51 (66.67%), Query Frame = -1 Query: 338 NSLNSRIFGLLSDAINSDFKTLLHHTEVRLLFKGKVLERVIHLKTKIISFL 490 N++NSR F L + + +D LL+H+EVR L +G VL R+ L+ ++ SFL Sbjct: 179 NAVNSRCFAQLCEGMEADHVKLLYHSEVRWLSRGLVLTRIFELRKEVFSFL 229
BLAST of SMED30029516 vs. Ensembl Medaka
Match: ENSORLT00000028620.1 (pep primary_assembly:ASM223467v1:3:35179252:35181570:1 gene:ENSORLG00000028714.1 transcript:ENSORLT00000028620.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 47.7506 bits (112), Expect = 1.690e-6 Identity = 21/51 (41.18%), Postives = 33/51 (64.71%), Query Frame = -1 Query: 338 NSLNSRIFGLLSDAINSDFKTLLHHTEVRLLFKGKVLERVIHLKTKIISFL 490 N++NSR F L + + +D LL+H+EVR L +G V R+ L+ ++ SFL Sbjct: 355 NAVNSRCFAQLCEGMEADHVKLLYHSEVRWLSRGLVFTRIFELRKEVFSFL 405
BLAST of SMED30029516 vs. Planmine SMEST
Match: SMESG000036914.1 (SMESG000036914.1) HSP 1 Score: 60.077 bits (144), Expect = 5.310e-11 Identity = 39/91 (42.86%), Postives = 56/91 (61.54%), Query Frame = -1 Query: 221 NSLNSRIFGLLSDAINSDFKTLLHHTEVRLLFKGKVLERVIHLKTKIISFLEVEDITLNFD-FRDSDWWLNGSAENIMTATSKLLSFKVKL 490 N+L SRIF L +A++SD+KTLL+HT V+ KGK+L R ++ K I +L V + F+ F D + L G+ N + TSKL F+ KL Sbjct: 153 NALQSRIFAALCEAMDSDYKTLLYHTVVQWHSKGKILNRFVYEKD--ICWLPVSFLNDFFEKFNDLNLKLQGAQGNFINITSKLKGFEEKL 241
BLAST of SMED30029516 vs. Planmine SMEST
Match: SMESG000028317.1 (SMESG000028317.1) HSP 1 Score: 46.9802 bits (110), Expect = 2.093e-6 Identity = 34/111 (30.63%), Postives = 56/111 (50.45%), Query Frame = -1 Query: 209 RIFGLLSDAINSDFKTLLHHTEVRLLFKGKVLERVIHLKTKIISFLEVEDITLNFDFRDSDWW---------------LNGS-----AENIMTATSKLLSF--KVKLVHQK 475 R+F + + NS F+ L++H E+R L KGK L R+ L ++ +FL E+ ++ F+++ W LN S + I+ A K+LSF K+ L+ QK Sbjct: 934 RLFARVYENSNSQFEILINHAEMRWLSKGKSLRRLFDLSDEVRTFLMAENPSMTDFFQNAQWLCTFAYLADIFEKLNDLNKSLQGKGCDKILFAYDKILSFVKKLALLQQK 1044
BLAST of SMED30029516 vs. Planmine SMEST
Match: SMESG000037770.1 (SMESG000037770.1) HSP 1 Score: 46.595 bits (109), Expect = 3.491e-6 Identity = 26/55 (47.27%), Postives = 38/55 (69.09%), Query Frame = -1 Query: 290 INSDFKTLLHHTEVRLLFKGKVLERVIHLKTKIISFLEVEDITLNF--DFRDSDW 448 + SD +L+HT VR L GKVL+RV +LK +I+ FLE++DIT+ F + S+W Sbjct: 832 LESDHSDVLYHTNVRWLSLGKVLKRVWNLKDEIVLFLEMKDITVEFTTQMKTSEW 886
BLAST of SMED30029516 vs. Planmine SMEST
Match: SMESG000024836.1 (SMESG000024836.1) HSP 1 Score: 44.669 bits (104), Expect = 6.289e-6 Identity = 24/54 (44.44%), Postives = 34/54 (62.96%), Query Frame = -1 Query: 326 SLNSRIFGLLSDAINSDFKTLLHHTEVRLLFKGKVLERVIHLKTKIISFLEVED 487 LN R F L + + SD++ L ++TE+R L KVL R + L KI+ FLEV+D Sbjct: 57 GLNHREFTTLLNDVESDYEDLPYYTEIRWLPSHKVLRRFLQLFDKIVLFLEVKD 110 The following BLAST results are available for this feature:
BLAST of SMED30029516 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 3
BLAST of SMED30029516 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30029516 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30029516 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30029516 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30029516 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30029516 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 1
BLAST of SMED30029516 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of SMED30029516 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30029516 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30029516 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30029516 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30029516 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 3
BLAST of SMED30029516 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 4
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30029516 ID=SMED30029516|Name=SMED30029516|organism=Schmidtea mediterranea sexual|type=transcript|length=517bpback to top Annotated Terms
The following terms have been associated with this transcript:
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