SMED30029432

Overview
NameSMED30029432
Smed IDSMED30029432
Length (bp)1064
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30029432 (SMED30029432) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30029432 (SMED30029432) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30029432 (SMED30029432) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30029432 (SMED30029432) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30029432

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 19

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
Smed sexual biotypeSMED30029432h1SMcG0017536 Contig48905uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30029432h1SMcG0017536 Contig48905newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
cephalic gangliaSMED30029432h1SMcG0017536 dd_Smed_v4_635_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30029432h1SMcG0017536 dd_Smed_v4_635_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30029432h1SMcG0017536 dd_Smed_v4_635_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30029432h1SMcG0017536 dd_Smed_v4_273_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30029432h1SMcG0017536 dd_Smed_v4_273_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30029432h1SMcG0017536 dd_Smed_v4_273_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30029432h1SMcG0017536 dd_Smed_v4_533_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30029432h1SMcG0017536 dd_Smed_v4_533_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30029432h1SMcG0017536 dd_Smed_v4_533_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30029432h1SMcG0017536 dd_Smed_v4_450_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30029432h1SMcG0017536 dd_Smed_v4_450_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30029432h1SMcG0017536 dd_Smed_v4_450_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynx musculatureSMED30029432h1SMcG0017536 dd_Smed_v6_273_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
musculature systemSMED30029432h1SMcG0017536 dd_Smed_v6_273_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynx musculatureSMED30029432h1SMcG0017536 dd_Smed_v6_450_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
musculature systemSMED30029432h1SMcG0017536 dd_Smed_v6_450_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parapharyngeal regionSMED30029432h1SMcG0017536 PL030005A20G01smed_ncbi_20240424PMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite colorimetric in situ hybridization evidence
Note: Hover over icons to view figure legend
Alignments
SMED30029432 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.001516:31138..32790 -5178
v31.001516:33313..36048 +2679
Homology
BLAST of SMED30029432 vs. Ensembl Human
Match: TAGLN (transgelin [Source:HGNC Symbol;Acc:HGNC:11553])

HSP 1 Score: 135.191 bits (339), Expect = 1.636e-37
Identity = 83/198 (41.92%), Postives = 116/198 (58.59%), Query Frame = -1
Query:  315 MSNRIGDMGMDVQINRKLNSKYDPESEKEVIDWLNKVSGEHVAL---GMQSVQQTLKNGQILIKLINKIYEGTDKKPAAALKIRCPLKFNTMSAPFKQMENIQLFLKASEAYGVPRASLFQTVDLYESRNMAQVLNTLLQLGT-ECQRNE--FKG-PTCGPKPTYENKREFTEEQLRSSQGIIGLQAGSNKGASQAGM 887
            M+N+    GM  ++  K+  KYD E E+ +++W+    G  V     G    Q  LKNG IL KL+N +Y      P  +  ++ P   N  S  FKQME +  FLKA+E YGV +  +FQTVDL+E ++MA V  TL+ LG+    +N+  ++G P    K   E+KREFTE QL+  + +IGLQ GSN+GASQAGM
Sbjct:    1 MANKGPSYGMSREVQSKIEKKYDEELEERLVEWIIVQCGPDVGRPDRGRLGFQVWLKNGVILSKLVNSLY------PDGSKPVKVPE--NPPSMVFKQMEQVAQFLKAAEDYGVIKTDMFQTVDLFEGKDMAAVQRTLMALGSLAVTKNDGHYRGDPNWFMKKAQEHKREFTESQLQEGKHVIGLQMGSNRGASQAGM 190          
BLAST of SMED30029432 vs. Ensembl Human
Match: TAGLN (transgelin [Source:HGNC Symbol;Acc:HGNC:11553])

HSP 1 Score: 135.191 bits (339), Expect = 1.636e-37
Identity = 83/198 (41.92%), Postives = 116/198 (58.59%), Query Frame = -1
Query:  315 MSNRIGDMGMDVQINRKLNSKYDPESEKEVIDWLNKVSGEHVAL---GMQSVQQTLKNGQILIKLINKIYEGTDKKPAAALKIRCPLKFNTMSAPFKQMENIQLFLKASEAYGVPRASLFQTVDLYESRNMAQVLNTLLQLGT-ECQRNE--FKG-PTCGPKPTYENKREFTEEQLRSSQGIIGLQAGSNKGASQAGM 887
            M+N+    GM  ++  K+  KYD E E+ +++W+    G  V     G    Q  LKNG IL KL+N +Y      P  +  ++ P   N  S  FKQME +  FLKA+E YGV +  +FQTVDL+E ++MA V  TL+ LG+    +N+  ++G P    K   E+KREFTE QL+  + +IGLQ GSN+GASQAGM
Sbjct:    1 MANKGPSYGMSREVQSKIEKKYDEELEERLVEWIIVQCGPDVGRPDRGRLGFQVWLKNGVILSKLVNSLY------PDGSKPVKVPE--NPPSMVFKQMEQVAQFLKAAEDYGVIKTDMFQTVDLFEGKDMAAVQRTLMALGSLAVTKNDGHYRGDPNWFMKKAQEHKREFTESQLQEGKHVIGLQMGSNRGASQAGM 190          
BLAST of SMED30029432 vs. Ensembl Human
Match: TAGLN (transgelin [Source:HGNC Symbol;Acc:HGNC:11553])

HSP 1 Score: 135.191 bits (339), Expect = 1.636e-37
Identity = 83/198 (41.92%), Postives = 116/198 (58.59%), Query Frame = -1
Query:  315 MSNRIGDMGMDVQINRKLNSKYDPESEKEVIDWLNKVSGEHVAL---GMQSVQQTLKNGQILIKLINKIYEGTDKKPAAALKIRCPLKFNTMSAPFKQMENIQLFLKASEAYGVPRASLFQTVDLYESRNMAQVLNTLLQLGT-ECQRNE--FKG-PTCGPKPTYENKREFTEEQLRSSQGIIGLQAGSNKGASQAGM 887
            M+N+    GM  ++  K+  KYD E E+ +++W+    G  V     G    Q  LKNG IL KL+N +Y      P  +  ++ P   N  S  FKQME +  FLKA+E YGV +  +FQTVDL+E ++MA V  TL+ LG+    +N+  ++G P    K   E+KREFTE QL+  + +IGLQ GSN+GASQAGM
Sbjct:    1 MANKGPSYGMSREVQSKIEKKYDEELEERLVEWIIVQCGPDVGRPDRGRLGFQVWLKNGVILSKLVNSLY------PDGSKPVKVPE--NPPSMVFKQMEQVAQFLKAAEDYGVIKTDMFQTVDLFEGKDMAAVQRTLMALGSLAVTKNDGHYRGDPNWFMKKAQEHKREFTESQLQEGKHVIGLQMGSNRGASQAGM 190          
BLAST of SMED30029432 vs. Ensembl Human
Match: TAGLN (transgelin [Source:HGNC Symbol;Acc:HGNC:11553])

HSP 1 Score: 135.191 bits (339), Expect = 1.636e-37
Identity = 83/198 (41.92%), Postives = 116/198 (58.59%), Query Frame = -1
Query:  315 MSNRIGDMGMDVQINRKLNSKYDPESEKEVIDWLNKVSGEHVAL---GMQSVQQTLKNGQILIKLINKIYEGTDKKPAAALKIRCPLKFNTMSAPFKQMENIQLFLKASEAYGVPRASLFQTVDLYESRNMAQVLNTLLQLGT-ECQRNE--FKG-PTCGPKPTYENKREFTEEQLRSSQGIIGLQAGSNKGASQAGM 887
            M+N+    GM  ++  K+  KYD E E+ +++W+    G  V     G    Q  LKNG IL KL+N +Y      P  +  ++ P   N  S  FKQME +  FLKA+E YGV +  +FQTVDL+E ++MA V  TL+ LG+    +N+  ++G P    K   E+KREFTE QL+  + +IGLQ GSN+GASQAGM
Sbjct:    1 MANKGPSYGMSREVQSKIEKKYDEELEERLVEWIIVQCGPDVGRPDRGRLGFQVWLKNGVILSKLVNSLY------PDGSKPVKVPE--NPPSMVFKQMEQVAQFLKAAEDYGVIKTDMFQTVDLFEGKDMAAVQRTLMALGSLAVTKNDGHYRGDPNWFMKKAQEHKREFTESQLQEGKHVIGLQMGSNRGASQAGM 190          
BLAST of SMED30029432 vs. Ensembl Human
Match: TAGLN (transgelin [Source:HGNC Symbol;Acc:HGNC:11553])

HSP 1 Score: 135.191 bits (339), Expect = 1.636e-37
Identity = 83/198 (41.92%), Postives = 116/198 (58.59%), Query Frame = -1
Query:  315 MSNRIGDMGMDVQINRKLNSKYDPESEKEVIDWLNKVSGEHVAL---GMQSVQQTLKNGQILIKLINKIYEGTDKKPAAALKIRCPLKFNTMSAPFKQMENIQLFLKASEAYGVPRASLFQTVDLYESRNMAQVLNTLLQLGT-ECQRNE--FKG-PTCGPKPTYENKREFTEEQLRSSQGIIGLQAGSNKGASQAGM 887
            M+N+    GM  ++  K+  KYD E E+ +++W+    G  V     G    Q  LKNG IL KL+N +Y      P  +  ++ P   N  S  FKQME +  FLKA+E YGV +  +FQTVDL+E ++MA V  TL+ LG+    +N+  ++G P    K   E+KREFTE QL+  + +IGLQ GSN+GASQAGM
Sbjct:    1 MANKGPSYGMSREVQSKIEKKYDEELEERLVEWIIVQCGPDVGRPDRGRLGFQVWLKNGVILSKLVNSLY------PDGSKPVKVPE--NPPSMVFKQMEQVAQFLKAAEDYGVIKTDMFQTVDLFEGKDMAAVQRTLMALGSLAVTKNDGHYRGDPNWFMKKAQEHKREFTESQLQEGKHVIGLQMGSNRGASQAGM 190          
BLAST of SMED30029432 vs. Ensembl Celegans
Match: cpn-1 (CPN-1; CalPoNin [Source:UniProtKB/TrEMBL;Acc:G5EFB5])

HSP 1 Score: 115.931 bits (289), Expect = 8.751e-31
Identity = 69/200 (34.50%), Postives = 110/200 (55.00%), Query Frame = -1
Query:  291 SNRIGDMGMDVQINRKLNSKYDPESEKEVIDWLNKVSGEHVAL--GMQSVQQTLKNGQILIKLINKIYEGTDKKPAAALKIRCPLKFNTMSAPFKQMENIQLFLKASEAYGVPRASLFQTVDLYESRNMAQVLNTLLQLGTECQRNEFKGPTCGPKPTYENKREFTEEQLRSSQGIIGLQAGSNKGASQAGMRIGTQRFI 884
            S+R    G+ ++  +K+  KYD     E++ W+  V+G+         ++ +  ++G +L  L N +  G+ KK             NT +  FK+MENI  FLK +E Y V ++ LFQTVDLYE ++   VL  L  L  + ++N F     GPK    ++RE+T+EQL++   +IGLQ GSNKGA+ +G+ +G  R +
Sbjct:    7 SDRAEKSGIALEAQQKIYEKYDKNLAGEILQWVQNVTGQSFDTQGDADNLVKVFQDGSLLCTLANSLKPGSVKK------------VNTSAMAFKKMENISFFLKFAEEY-VQKSELFQTVDLYEGQDPNAVLICLASLARKSEKN-FGRSGLGPKEAQGDRREWTDEQLKAGHNVIGLQMGSNKGATASGLNMGNTRHM 192          
BLAST of SMED30029432 vs. Ensembl Celegans
Match: cpn-1 (CPN-1; CalPoNin [Source:UniProtKB/TrEMBL;Acc:G5EFB5])

HSP 1 Score: 115.931 bits (289), Expect = 8.751e-31
Identity = 69/200 (34.50%), Postives = 110/200 (55.00%), Query Frame = -1
Query:  291 SNRIGDMGMDVQINRKLNSKYDPESEKEVIDWLNKVSGEHVAL--GMQSVQQTLKNGQILIKLINKIYEGTDKKPAAALKIRCPLKFNTMSAPFKQMENIQLFLKASEAYGVPRASLFQTVDLYESRNMAQVLNTLLQLGTECQRNEFKGPTCGPKPTYENKREFTEEQLRSSQGIIGLQAGSNKGASQAGMRIGTQRFI 884
            S+R    G+ ++  +K+  KYD     E++ W+  V+G+         ++ +  ++G +L  L N +  G+ KK             NT +  FK+MENI  FLK +E Y V ++ LFQTVDLYE ++   VL  L  L  + ++N F     GPK    ++RE+T+EQL++   +IGLQ GSNKGA+ +G+ +G  R +
Sbjct:    7 SDRAEKSGIALEAQQKIYEKYDKNLAGEILQWVQNVTGQSFDTQGDADNLVKVFQDGSLLCTLANSLKPGSVKK------------VNTSAMAFKKMENISFFLKFAEEY-VQKSELFQTVDLYEGQDPNAVLICLASLARKSEKN-FGRSGLGPKEAQGDRREWTDEQLKAGHNVIGLQMGSNKGATASGLNMGNTRHM 192          
BLAST of SMED30029432 vs. Ensembl Celegans
Match: cpn-1 (CPN-1; CalPoNin [Source:UniProtKB/TrEMBL;Acc:G5EFB5])

HSP 1 Score: 115.931 bits (289), Expect = 8.751e-31
Identity = 69/200 (34.50%), Postives = 110/200 (55.00%), Query Frame = -1
Query:  291 SNRIGDMGMDVQINRKLNSKYDPESEKEVIDWLNKVSGEHVAL--GMQSVQQTLKNGQILIKLINKIYEGTDKKPAAALKIRCPLKFNTMSAPFKQMENIQLFLKASEAYGVPRASLFQTVDLYESRNMAQVLNTLLQLGTECQRNEFKGPTCGPKPTYENKREFTEEQLRSSQGIIGLQAGSNKGASQAGMRIGTQRFI 884
            S+R    G+ ++  +K+  KYD     E++ W+  V+G+         ++ +  ++G +L  L N +  G+ KK             NT +  FK+MENI  FLK +E Y V ++ LFQTVDLYE ++   VL  L  L  + ++N F     GPK    ++RE+T+EQL++   +IGLQ GSNKGA+ +G+ +G  R +
Sbjct:    7 SDRAEKSGIALEAQQKIYEKYDKNLAGEILQWVQNVTGQSFDTQGDADNLVKVFQDGSLLCTLANSLKPGSVKK------------VNTSAMAFKKMENISFFLKFAEEY-VQKSELFQTVDLYEGQDPNAVLICLASLARKSEKN-FGRSGLGPKEAQGDRREWTDEQLKAGHNVIGLQMGSNKGATASGLNMGNTRHM 192          
BLAST of SMED30029432 vs. Ensembl Celegans
Match: cpn-2 (Transgelin [Source:UniProtKB/TrEMBL;Acc:A0A0K3AWU9])

HSP 1 Score: 91.6633 bits (226), Expect = 1.158e-21
Identity = 63/203 (31.03%), Postives = 99/203 (48.77%), Query Frame = -1
Query:  315 MSNRIGDMGMDVQINRKLNSKYDPESEKEVIDWLNKVSGEHVALGMQSVQQT------LKNGQILIKLINKIYEGT----DKKPAAALKIRCPLKFNTMSAPFKQMENIQLFLKASEAYGVPRASLFQTVDLYESRNMAQVLNTLLQLGTECQ--RNEFKGPTCGPKPTYENKREFTEEQLRSSQGIIGLQAGSNKGASQAGM 887
            M+N     G+  ++ +K ++++  E   EV+ W+  V+GE  +  + + + +      LK+G +L KLI K+        +KKP  A               F  MENI  FL A++ +GV   S FQTVDLYE++   +V+  L  L    Q   +  + P    K    + R+F E  +R  + +I LQ G+NK ASQ GM
Sbjct:    1 MANHGPSYGLSRELQKKNDARFVLEEAIEVLKWIENVTGERFSFDVTTCESSTDVSNLLKDGVMLCKLIEKLDPSCRVVYNKKPKMA---------------FPMMENISNFLAAAKQFGVMEISCFQTVDLYENKQCYKVIECLRLLAAVAQSRSSHLEHPAWVVKLAQSSPRQFPEAVMRRGEMVIPLQYGTNKCASQKGM 188          
BLAST of SMED30029432 vs. Ensembl Celegans
Match: cpn-2 (Transgelin [Source:UniProtKB/TrEMBL;Acc:A0A0K3AWU9])

HSP 1 Score: 91.6633 bits (226), Expect = 1.158e-21
Identity = 63/203 (31.03%), Postives = 99/203 (48.77%), Query Frame = -1
Query:  315 MSNRIGDMGMDVQINRKLNSKYDPESEKEVIDWLNKVSGEHVALGMQSVQQT------LKNGQILIKLINKIYEGT----DKKPAAALKIRCPLKFNTMSAPFKQMENIQLFLKASEAYGVPRASLFQTVDLYESRNMAQVLNTLLQLGTECQ--RNEFKGPTCGPKPTYENKREFTEEQLRSSQGIIGLQAGSNKGASQAGM 887
            M+N     G+  ++ +K ++++  E   EV+ W+  V+GE  +  + + + +      LK+G +L KLI K+        +KKP  A               F  MENI  FL A++ +GV   S FQTVDLYE++   +V+  L  L    Q   +  + P    K    + R+F E  +R  + +I LQ G+NK ASQ GM
Sbjct:    1 MANHGPSYGLSRELQKKNDARFVLEEAIEVLKWIENVTGERFSFDVTTCESSTDVSNLLKDGVMLCKLIEKLDPSCRVVYNKKPKMA---------------FPMMENISNFLAAAKQFGVMEISCFQTVDLYENKQCYKVIECLRLLAAVAQSRSSHLEHPAWVVKLAQSSPRQFPEAVMRRGEMVIPLQYGTNKCASQKGM 188          
BLAST of SMED30029432 vs. Ensembl Fly
Match: Mp20 (gene:FBgn0002789 transcript:FBtr0087745)

HSP 1 Score: 156.377 bits (394), Expect = 2.678e-46
Identity = 88/193 (45.60%), Postives = 118/193 (61.14%), Query Frame = -1
Query:  291 MGMDVQINRKLNSKYDPESEKEVIDWLNKVSGEHVALGMQSVQQTLKNGQILIKLINKIYEGTDKKPAAALKIRCPLKFNTMSAPFKQMENIQLFLKASEAYGVPRASLFQTVDLYESRNMAQVLNTLLQLGTECQRN-EFKGPTCGPKPTYENKREFTEEQLRSSQGIIGLQAGSNKGASQAGMRIGTQRFI 866
            M ++  +  K+ SK +PE +KE  +W+  +  E    G QS +  LK+GQ+L KLIN +       P A  K+      N+    FK MENI  F KA + YGVP   +FQTVDLYE +++A V NT+  LG    ++ +FKGP  GPKP  E KR+FTEEQL++ Q I+GLQAGSNKGA+QAG  +G  R I
Sbjct:    1 MSLERAVRAKIASKRNPEMDKEAQEWIEAIIAEKFPAG-QSYEDVLKDGQVLCKLINVL------SPNAVPKV------NSSGGQFKFMENINNFQKALKEYGVPDIDVFQTVDLYEKKDIANVTNTIFALGRATYKHADFKGPFLGPKPADECKRDFTEEQLKAGQTIVGLQAGSNKGATQAGQNLGAGRKI 180          
BLAST of SMED30029432 vs. Ensembl Fly
Match: Mp20 (gene:FBgn0002789 transcript:FBtr0345532)

HSP 1 Score: 156.377 bits (394), Expect = 3.215e-46
Identity = 88/193 (45.60%), Postives = 118/193 (61.14%), Query Frame = -1
Query:  291 MGMDVQINRKLNSKYDPESEKEVIDWLNKVSGEHVALGMQSVQQTLKNGQILIKLINKIYEGTDKKPAAALKIRCPLKFNTMSAPFKQMENIQLFLKASEAYGVPRASLFQTVDLYESRNMAQVLNTLLQLGTECQRN-EFKGPTCGPKPTYENKREFTEEQLRSSQGIIGLQAGSNKGASQAGMRIGTQRFI 866
            M ++  +  K+ SK +PE +KE  +W+  +  E    G QS +  LK+GQ+L KLIN +       P A  K+      N+    FK MENI  F KA + YGVP   +FQTVDLYE +++A V NT+  LG    ++ +FKGP  GPKP  E KR+FTEEQL++ Q I+GLQAGSNKGA+QAG  +G  R I
Sbjct:    1 MSLERAVRAKIASKRNPEMDKEAQEWIEAIIAEKFPAG-QSYEDVLKDGQVLCKLINVL------SPNAVPKV------NSSGGQFKFMENINNFQKALKEYGVPDIDVFQTVDLYEKKDIANVTNTIFALGRATYKHADFKGPFLGPKPADECKRDFTEEQLKAGQTIVGLQAGSNKGATQAGQNLGAGRKI 180          
BLAST of SMED30029432 vs. Ensembl Fly
Match: Chd64 (gene:FBgn0035499 transcript:FBtr0073270)

HSP 1 Score: 126.716 bits (317), Expect = 9.121e-35
Identity = 74/200 (37.00%), Postives = 107/200 (53.50%), Query Frame = -1
Query:  291 SNRIGDMGMDVQINRKLNSKYDPESEKEVIDWLNKVSGEHVALG--MQSVQQTLKNGQILIKLINKIYEGTDKKPAAALKIRCPLKFNTMSAPFKQMENIQLFLKASEAYGVPRASLFQTVDLYESRNMAQVLNTLLQLGTECQRNEFKGPTCGPKPTYENKREFTEEQLRSSQGIIGLQAGSNKGASQAGMRIGTQRFI 884
            SNR    G   +  RK+NSKY  E  +E ++W+  V+ E +       +  + LK+G IL KL N +  G+ KK             N     FK MENI  FL  ++ +GVP    FQ+VDL+E +N+  V+  L  LG +   + F  P+ GPK   +N R F++EQLR+   +I LQ GSNKGA+Q+G+  G  R +
Sbjct:    3 SNRAAKSGFAAEAQRKINSKYSEELAQESLEWIKAVTEEPINTSGDTDNFFEVLKDGVILCKLANALQPGSIKK------------INESKMAFKCMENISAFLACAKNFGVPTQETFQSVDLWERQNLNSVVICLQSLGRKA--SNFNKPSIGPKEADKNVRNFSDEQLRAGANVISLQYGSNKGATQSGINFGNTRHM 188          
BLAST of SMED30029432 vs. Ensembl Fly
Match: CG5023 (gene:FBgn0038774 transcript:FBtr0083866)

HSP 1 Score: 124.02 bits (310), Expect = 5.542e-34
Identity = 75/167 (44.91%), Postives = 97/167 (58.08%), Query Frame = -1
Query:  318 ESEKEVIDWLNKVSGEHVALGMQSVQQTLKNGQILIKLINKIYEGTDKKPAAALKIRCPLKFNTMSAPFKQMENIQLFLKASEAYGVPRASLFQTVDLYESRNMAQVLNTLLQLGTECQRN-EFKGPTCGPKPTYENKREFTEEQLRSSQGIIGLQAGSNKGASQAG 815
            E E+EV++W+  V GE V  G    +  LK+G  L KL NK+  G+ KK                   F+ MENIQ F  A + YGVP   +FQT DL+E RN+ QV  +L  LG   Q++ E+ GPT GPK   +N+R FTEEQLR+ +G + LQ G NKGASQAG
Sbjct:    7 EQEQEVLNWVFAVIGEKVPSG--QYEDILKDGIWLCKLANKLAPGSVKK------------IQERGTNFQLMENIQRFQAAVKKYGVPEEEIFQTADLFERRNIPQVTLSLYALGRITQKHPEYTGPTLGPKMADKNERSFTEEQLRAHEGELNLQMGFNKGASQAG 159          
BLAST of SMED30029432 vs. Ensembl Fly
Match: Chd64 (gene:FBgn0035499 transcript:FBtr0310531)

HSP 1 Score: 117.472 bits (293), Expect = 1.854e-31
Identity = 69/185 (37.30%), Postives = 100/185 (54.05%), Query Frame = -1
Query:  291 KLNSKYDPESEKEVIDWLNKVSGEHVALG--MQSVQQTLKNGQILIKLINKIYEGTDKKPAAALKIRCPLKFNTMSAPFKQMENIQLFLKASEAYGVPRASLFQTVDLYESRNMAQVLNTLLQLGTECQRNEFKGPTCGPKPTYENKREFTEEQLRSSQGIIGLQAGSNKGASQAGMRIGTQRFI 839
            K+NSKY  E  +E ++W+  V+ E +       +  + LK+G IL KL N +  G+ KK             N     FK MENI  FL  ++ +GVP    FQ+VDL+E +N+  V+  L  LG +     F  P+ GPK   +N R F++EQLR+   +I LQ GSNKGA+Q+G+  G  R +
Sbjct:    5 KINSKYSEELAQESLEWIKAVTEEPINTSGDTDNFFEVLKDGVILCKLANALQPGSIKK------------INESKMAFKCMENISAFLACAKNFGVPTQETFQSVDLWERQNLNSVVICLQSLGRKASN--FNKPSIGPKEADKNVRNFSDEQLRAGANVISLQYGSNKGATQSGINFGNTRHM 175          
BLAST of SMED30029432 vs. Ensembl Zebrafish
Match: tagln2 (transgelin 2 [Source:ZFIN;Acc:ZDB-GENE-020802-2])

HSP 1 Score: 150.214 bits (378), Expect = 2.227e-43
Identity = 84/204 (41.18%), Postives = 120/204 (58.82%), Query Frame = -1
Query:  315 VINMSNRIGDMGMDVQINRKLNSKYDPESEKEVIDWLNKVSGEHVAL---GMQSVQQTLKNGQILIKLINKIYEGTDKKPAAALKIRCPLKFNTMSAPFKQMENIQLFLKASEAYGVPRASLFQTVDLYESRNMAQVLNTLLQLGT-------ECQRNEFKGPTCGPKPTYENKREFTEEQLRSSQGIIGLQAGSNKGASQAGM 896
             + M+N+    G+  ++  K++ KYDPE E  ++ W+    GE +     G Q  QQ LK+G IL +LIN +++  D KP          K  + S  FKQME I  FL A+E YG+ ++ +FQTVDL+E +++A V  TLL LG+        C R +   P   PK + EN+REF+EEQ++  Q +IGLQ G+NKGASQAGM
Sbjct:   13 TVKMANKGPSYGLSREVQSKIDKKYDPELEGRLVQWIVSQCGEAIGKPQPGKQGFQQWLKDGCILCELINSLFK--DSKPVK--------KIQSSSMAFKQMEQISQFLTAAERYGITKSDIFQTVDLWEGKDLAAVQMTLLSLGSLAVTKDDGCYRGD---PAWFPKKSQENRREFSEEQMKEGQSVIGLQMGTNKGASQAGM 203          
BLAST of SMED30029432 vs. Ensembl Zebrafish
Match: tagln2 (transgelin 2 [Source:ZFIN;Acc:ZDB-GENE-020802-2])

HSP 1 Score: 149.058 bits (375), Expect = 5.072e-43
Identity = 84/201 (41.79%), Postives = 119/201 (59.20%), Query Frame = -1
Query:  315 MSNRIGDMGMDVQINRKLNSKYDPESEKEVIDWLNKVSGEHVAL---GMQSVQQTLKNGQILIKLINKIYEGTDKKPAAALKIRCPLKFNTMSAPFKQMENIQLFLKASEAYGVPRASLFQTVDLYESRNMAQVLNTLLQLGT-------ECQRNEFKGPTCGPKPTYENKREFTEEQLRSSQGIIGLQAGSNKGASQAGM 887
            M+N+    G+  ++  K++ KYDPE E  ++ W+    GE +     G Q  QQ LK+G IL +LIN +++  D KP          K  + S  FKQME I  FL A+E YG+ ++ +FQTVDL+E +++A V  TLL LG+        C R +   P   PK + EN+REF+EEQ++  Q +IGLQ G+NKGASQAGM
Sbjct:    1 MANKGPSYGLSREVQSKIDKKYDPELEGRLVQWIVSQCGEAIGKPQPGKQGFQQWLKDGCILCELINSLFK--DSKPVK--------KIQSSSMAFKQMEQISQFLTAAERYGITKSDIFQTVDLWEGKDLAAVQMTLLSLGSLAVTKDDGCYRGD---PAWFPKKSQENRREFSEEQMKEGQSVIGLQMGTNKGASQAGM 188          
BLAST of SMED30029432 vs. Ensembl Zebrafish
Match: tagln2 (transgelin 2 [Source:ZFIN;Acc:ZDB-GENE-020802-2])

HSP 1 Score: 149.058 bits (375), Expect = 5.072e-43
Identity = 84/201 (41.79%), Postives = 119/201 (59.20%), Query Frame = -1
Query:  315 MSNRIGDMGMDVQINRKLNSKYDPESEKEVIDWLNKVSGEHVAL---GMQSVQQTLKNGQILIKLINKIYEGTDKKPAAALKIRCPLKFNTMSAPFKQMENIQLFLKASEAYGVPRASLFQTVDLYESRNMAQVLNTLLQLGT-------ECQRNEFKGPTCGPKPTYENKREFTEEQLRSSQGIIGLQAGSNKGASQAGM 887
            M+N+    G+  ++  K++ KYDPE E  ++ W+    GE +     G Q  QQ LK+G IL +LIN +++  D KP          K  + S  FKQME I  FL A+E YG+ ++ +FQTVDL+E +++A V  TLL LG+        C R +   P   PK + EN+REF+EEQ++  Q +IGLQ G+NKGASQAGM
Sbjct:    1 MANKGPSYGLSREVQSKIDKKYDPELEGRLVQWIVSQCGEAIGKPQPGKQGFQQWLKDGCILCELINSLFK--DSKPVK--------KIQSSSMAFKQMEQISQFLTAAERYGITKSDIFQTVDLWEGKDLAAVQMTLLSLGSLAVTKDDGCYRGD---PAWFPKKSQENRREFSEEQMKEGQSVIGLQMGTNKGASQAGM 188          
BLAST of SMED30029432 vs. Ensembl Zebrafish
Match: tagln3a (transgelin 3a [Source:ZFIN;Acc:ZDB-GENE-060531-53])

HSP 1 Score: 133.65 bits (335), Expect = 4.448e-37
Identity = 79/198 (39.90%), Postives = 115/198 (58.08%), Query Frame = -1
Query:  315 MSNRIGDMGMDVQINRKLNSKYDPESEKEVIDWLNKVSGEHVAL---GMQSVQQTLKNGQILIKLINKIYEGTDKKPAAALKIRCPLKFNTMSAPFKQMENIQLFLKASEAYGVPRASLFQTVDLYESRNMAQVLNTLLQLGTEC---QRNEFKG-PTCGPKPTYENKREFTEEQLRSSQGIIGLQAGSNKGASQAGM 887
            M+NR    G+  ++  K++ KYD E E  ++DW+    G+ +     G Q+ Q+ L +G IL +LIN +Y      P+    I+   K       FKQME I  FL+ +E YGV R  +FQTVDL+E ++MA V  TL+ LG+E        ++G P    + T  +KREF+EEQLR  + +IG+Q GSN+GASQ+GM
Sbjct:    1 MANRGPSYGLSREVQEKIDQKYDAELESRLVDWILIQCGDDLQRPEPGKQNFQKWLMSGTILCRLINSLY------PSGEEPIK---KITESKMVFKQMEKISQFLQFAEEYGVNRGDIFQTVDLWEGKDMAAVQRTLMALGSEALTKDDGHYRGDPDWFHRKTKGHKREFSEEQLRQGRVVIGMQMGSNRGASQSGM 189          
BLAST of SMED30029432 vs. Ensembl Zebrafish
Match: tagln3b (transgelin 3b [Source:ZFIN;Acc:ZDB-GENE-030131-4199])

HSP 1 Score: 132.494 bits (332), Expect = 8.415e-37
Identity = 82/199 (41.21%), Postives = 112/199 (56.28%), Query Frame = -1
Query:  315 MSNRIGDMGMDVQINRKLNSKYDPESEKEVIDWLNKVSGEHVAL---GMQSVQQTLKNGQILIKLINKIYEGTDKKPAAALKIRCPLKFNTMSAPFKQMENIQLFLKASEAYGVPRASLFQTVDLYESRNMAQVLNTLLQLGTECQRNEFKGPTCGPKPTYE-----NKREFTEEQLRSSQGIIGLQAGSNKGASQAGM 887
            M+NR    G+  ++  K+  KYDPE E  + DW+    G +V     G Q+ Q  L +G IL +LIN +Y      P     I+   K +     FKQME I  FL+A+EAYGV    +FQTVDL+E ++MA V  TL+ LG+     +  G   G +  +      N+REF+EEQLR  Q +IGLQ GSN+GASQ+GM
Sbjct:    1 MANRGPSYGLSREVQEKIEQKYDPELESRLEDWIVAQCGGNVEKPQPGKQNFQNWLMDGTILCRLINSLY------PRGKEPIK---KISETQMAFKQMEKISQFLQAAEAYGVITTDIFQTVDLWEGKDMAAVQRTLMALGSVAITKD-DGQYRGNREWFHRKSQGNRREFSEEQLRQGQNLIGLQMGSNRGASQSGM 189          
BLAST of SMED30029432 vs. Ensembl Xenopus
Match: tagln2 (transgelin 2 [Source:NCBI gene;Acc:394981])

HSP 1 Score: 137.117 bits (344), Expect = 2.383e-38
Identity = 80/199 (40.20%), Postives = 113/199 (56.78%), Query Frame = -1
Query:  315 MSNRIGDMGMDVQINRKLNSKYDPESEKEVIDWLNKVSGEHVAL----GMQSVQQTLKNGQILIKLINKIYEGTDKKPAAALKIRCPLKFNTMSAPFKQMENIQLFLKASEAYGVPRASLFQTVDLYESRNMAQVLNTLLQLG---TECQRNEFKG-PTCGPKPTYENKREFTEEQLRSSQGIIGLQAGSNKGASQAGM 887
            M+N+    G+  ++ +K++ KYD + E  ++ W+    G H       G  +VQ  LK+G +L  LIN +  G+              K  T +  FKQME I  FLKA E YG+P + LFQTVDL+E ++MA V  TL+ LG          F+G P   PK + ENKREF++++L+  Q IIGLQ G+NKGASQ+GM
Sbjct:    1 MANKGPAYGLSREVQQKIDQKYDNDLETILVQWIKAQCGTHAGSFDGQGKSAVQVWLKDGTVLSHLINSLAPGS------------IAKVQTSAMAFKQMEQISQFLKACERYGIPASDLFQTVDLWEGKDMASVQRTLMNLGGIAVTKGDGYFRGDPNWFPKKSQENKREFSQDKLKEGQNIIGLQMGTNKGASQSGM 187          
BLAST of SMED30029432 vs. Ensembl Xenopus
Match: kcnb2 (potassium channel, voltage gated Shab related subfamily B, member 2 [Source:Xenbase;Acc:XB-GENE-989392])

HSP 1 Score: 133.265 bits (334), Expect = 6.655e-37
Identity = 79/198 (39.90%), Postives = 119/198 (60.10%), Query Frame = -1
Query:  315 MSNRIGDMGMDVQINRKLNSKYDPESEKEVIDWLNKVSGEHVAL---GMQSVQQTLKNGQILIKLINKIYEGTDKKPAAALKIRCPLKFNTMSAPFKQMENIQLFLKASEAYGVPRASLFQTVDLYESRNMAQVLNTLLQLGT-ECQRNE--FKG-PTCGPKPTYENKREFTEEQLRSSQGIIGLQAGSNKGASQAGM 887
            M+N+    GM  ++  ++  KYD E E+ ++ W+    G+ V     G    QQ LKNG +L KLIN +Y      P  +  ++ P    +M   FKQME +  FL+ASE YGV +  +FQTVDLYE ++MA V  T++ LG+    +N+  +KG P+   K   E+KR+F++E+L+  + IIGLQ GSN+GA+Q+GM
Sbjct:    1 MANKGPSYGMSREVQSRIEKKYDEELEQRLVQWICIQCGDDVGTPEQGRLGFQQWLKNGLVLSKLINSLY------PKGSQPVKIPDPPPSMV--FKQMEQVAQFLRASEDYGVVKTDMFQTVDLYEGKDMAAVQRTIVALGSIAVTKNDGQYKGDPSWFMKKAQEHKRDFSDEKLKEGKNIIGLQMGSNQGATQSGM 190          
BLAST of SMED30029432 vs. Ensembl Xenopus
Match: kcnb2 (potassium channel, voltage gated Shab related subfamily B, member 2 [Source:Xenbase;Acc:XB-GENE-989392])

HSP 1 Score: 133.265 bits (334), Expect = 7.224e-37
Identity = 79/198 (39.90%), Postives = 119/198 (60.10%), Query Frame = -1
Query:  315 MSNRIGDMGMDVQINRKLNSKYDPESEKEVIDWLNKVSGEHVAL---GMQSVQQTLKNGQILIKLINKIYEGTDKKPAAALKIRCPLKFNTMSAPFKQMENIQLFLKASEAYGVPRASLFQTVDLYESRNMAQVLNTLLQLGT-ECQRNE--FKG-PTCGPKPTYENKREFTEEQLRSSQGIIGLQAGSNKGASQAGM 887
            M+N+    GM  ++  ++  KYD E E+ ++ W+    G+ V     G    QQ LKNG +L KLIN +Y      P  +  ++ P    +M   FKQME +  FL+ASE YGV +  +FQTVDLYE ++MA V  T++ LG+    +N+  +KG P+   K   E+KR+F++E+L+  + IIGLQ GSN+GA+Q+GM
Sbjct:    7 MANKGPSYGMSREVQSRIEKKYDEELEQRLVQWICIQCGDDVGTPEQGRLGFQQWLKNGLVLSKLINSLY------PKGSQPVKIPDPPPSMV--FKQMEQVAQFLRASEDYGVVKTDMFQTVDLYEGKDMAAVQRTIVALGSIAVTKNDGQYKGDPSWFMKKAQEHKRDFSDEKLKEGKNIIGLQMGSNQGATQSGM 196          
BLAST of SMED30029432 vs. Ensembl Xenopus
Match: tfdp1 (transcription factor Dp-1 [Source:Xenbase;Acc:XB-GENE-1219073])

HSP 1 Score: 120.939 bits (302), Expect = 5.638e-31
Identity = 85/238 (35.71%), Postives = 132/238 (55.46%), Query Frame = -1
Query:  159 GMDVQINRKLNSKYDPESEKEVIDWLNKVSGEHVALGMQSVQQTLKNGQILIKLINKIYEGTDKKPAAALKIRCPLKFNTMSAPFKQMENIQLFLKASEAYGVPRASLFQTVDLYESRNMAQVLNTLLQL-GTECQRNEFKGPTCGPKPTYENKREFTEEQLRSSQGIIGLQAGSNKGASQAGM-RIGTQRFINDVTTGVEMSDEGKTTIGLQMGPDKSKVDSQSGM-SFGAQRHITD 863
            G+  ++  K+  KYDP+ E+E+  W+ +V+G  +    ++ QQ L++G IL  LINK+  G+ KK             N     + ++ENI  F+KA + YG+    +F+  DL+E+ NM QV  +L+ L G    R        G K   + KR+F +E++++ Q +IGLQ G+NK ASQAGM   GT+R + D     +   + +TTI LQMG +K    SQ+GM + G +R I D
Sbjct:   11 GLSAEVKNKIAQKYDPQVEEELRLWIEEVTGMIIG---ENFQQGLRDGIILCNLINKLQPGSIKK------------INEAKLNWHKLENIGNFIKAMQDYGMKPHDIFEANDLFENGNMTQVQTSLVSLAGLAKTRGFHTSVDIGVKYAEKQKRQFGDEKMKAGQSVIGLQMGTNKCASQAGMTAYGTRRHLYDPKMQTDKPFD-QTTISLQMGTNKGA--SQAGMPAPGTRRDIYD 230          
BLAST of SMED30029432 vs. Ensembl Xenopus
Match: cnn1 (calponin 1, basic, smooth muscle [Source:NCBI gene;Acc:407907])

HSP 1 Score: 113.62 bits (283), Expect = 1.998e-28
Identity = 82/239 (34.31%), Postives = 126/239 (52.72%), Query Frame = -1
Query:  156 GMDVQINRKLNSKYDPESEKEVIDWLNKVSGEHVALGMQSVQQTLKNGQILIKLINKIYEGTDKKPAAALKIRCPLKFNTMSAPFKQMENIQLFLKASEAYGVPRASLFQTVDLYESRNMAQVLNTLLQL-GTECQRNEFKGPTCGPKPTYENKREFTEEQLRSSQGIIGLQAGSNKGASQAGMR-IGTQRFINDVTTGVEMSDEGKTTIGLQMGPDKSKVDSQSGMSF-GAQRHITDS 863
            G+  ++  KL  KYDP+ E E+  W+ +V+G  +       Q+ LK+G IL +L+NK+      +P +  K+      N     + Q+EN+  F+KA   YG+    LF+  DL+E+ NM QV  +LL L G    R        G K + + +R F +   ++   +IGLQ G+NK ASQ+GM   GT+R + D    + +     +TI LQMG +K    SQ GM+  G +RHI D+
Sbjct:   13 GLSAEVRNKLAQKYDPQKETELKVWIEEVTGMSIG---PDFQKGLKDGVILCELMNKL------RPHSIPKV------NVSRQNWHQLENLSNFIKAMNLYGMKSVDLFEANDLFENGNMTQVQVSLLALAGLAKSRGMQSEVDIGVKYSEKQERNFDDNTKKAGHCVIGLQMGTNKCASQSGMTAYGTRRHLYDPKNTI-LPPMDHSTISLQMGSNKGA--SQVGMTAPGTRRHIYDT 233          
BLAST of SMED30029432 vs. Ensembl Mouse
Match: Tagln3 (transgelin 3 [Source:MGI Symbol;Acc:MGI:1926784])

HSP 1 Score: 135.191 bits (339), Expect = 9.858e-38
Identity = 82/202 (40.59%), Postives = 111/202 (54.95%), Query Frame = -1
Query:  315 MSNRIGDMGMDVQINRKLNSKYDPESEKEVIDWLNKVSGE---HVALGMQSVQQTLKNGQILIKLINKIYE-GTDKKPAAALKIRCPLKFNTMSAPFKQMENIQLFLKASEAYGVPRASLFQTVDLYESRNMAQVLNTLLQLGTE-------CQRNEFKGPTCGPKPTYENKREFTEEQLRSSQGIIGLQAGSNKGASQAGM 887
            M+NR    G+  ++  K+  KYD + E +++DW+     E   H   G    Q+ L +G +L KLIN +Y  G +  P          K +     FKQME I  FLKA+E YGV    +FQTVDL+E ++MA V  TL+ LG+        C R E   P+   +   +N+R F+EEQLR  Q +IGLQ GSNKGASQAGM
Sbjct:    1 MANRGPSYGLSREVQEKIEQKYDADLENKLVDWIILQCAEDIEHPPPGRAHFQKWLMDGTVLCKLINSLYPPGQEPIP----------KISESKMAFKQMEQISQFLKAAEVYGVRTTDIFQTVDLWEGKDMAAVQRTLMALGSVAVTKDDGCYRGE---PSWFHRKAQQNRRGFSEEQLRQGQNVIGLQMGSNKGASQAGM 189          
BLAST of SMED30029432 vs. Ensembl Mouse
Match: Tagln (transgelin [Source:MGI Symbol;Acc:MGI:106012])

HSP 1 Score: 134.42 bits (337), Expect = 1.988e-37
Identity = 82/198 (41.41%), Postives = 116/198 (58.59%), Query Frame = -1
Query:  315 MSNRIGDMGMDVQINRKLNSKYDPESEKEVIDWLNKVSGEHVAL---GMQSVQQTLKNGQILIKLINKIYEGTDKKPAAALKIRCPLKFNTMSAPFKQMENIQLFLKASEAYGVPRASLFQTVDLYESRNMAQVLNTLLQLGT-ECQRNE--FKG-PTCGPKPTYENKREFTEEQLRSSQGIIGLQAGSNKGASQAGM 887
            M+N+    GM  ++  K+  KYD E E+ +++W+    G  V     G    Q  LKNG IL KL+N +Y      P  +  ++ P   N  S  FKQME +  FLKA+E YGV +  +FQTVDLYE ++MA V  TL+ LG+    +N+  ++G P    K   E+KR+FT+ QL+  + +IGLQ GSN+GASQAGM
Sbjct:    1 MANKGPSYGMSREVQSKIEKKYDEELEERLVEWIVVQCGPDVGRPDRGRLGFQVWLKNGVILSKLVNSLY------PEGSKPVKVPE--NPPSMVFKQMEQVAQFLKAAEDYGVIKTDMFQTVDLYEGKDMAAVQRTLMALGSLAVTKNDGNYRGDPNWFMKKAQEHKRDFTDSQLQEGKHVIGLQMGSNRGASQAGM 190          
BLAST of SMED30029432 vs. Ensembl Mouse
Match: Tagln2 (transgelin 2 [Source:MGI Symbol;Acc:MGI:1312985])

HSP 1 Score: 129.028 bits (323), Expect = 1.801e-35
Identity = 78/198 (39.39%), Postives = 114/198 (57.58%), Query Frame = -1
Query:  315 MSNRIGDMGMDVQINRKLNSKYDPESEKEVIDWLNKVSGEHVAL---GMQSVQQTLKNGQILIKLINKIYEGTDKKPAAALKIRCPLKFNTMSAPFKQMENIQLFLKASEAYGVPRASLFQTVDLYESRNMAQVLNTLLQL-GTECQRNE--FKG-PTCGPKPTYENKREFTEEQLRSSQGIIGLQAGSNKGASQAGM 887
            M+NR    G+  ++ +K+  +YD + E+ +I W+     E V     G ++ Q+ LK+G +L KLIN +Y      P     ++   K    S  FKQME I  FL+A+E YG+    +FQTVDL+E +NMA V  TL+ L G    R++  F G P   PK + EN R F++ QL+  + +IGLQ G+N+GASQAGM
Sbjct:    1 MANRGPSYGLSREVQQKIEKQYDADLEQILIQWITTQCREDVGQPQPGRENFQKWLKDGTVLCKLINSLY------PEGQAPVK---KIQASSMAFKQMEQISQFLQAAERYGINTTDIFQTVDLWEGKNMACVQRTLMNLGGLAVARDDGLFSGDPNWFPKKSKENPRNFSDNQLQEGKNVIGLQMGTNRGASQAGM 189          
BLAST of SMED30029432 vs. Ensembl Mouse
Match: Tagln2 (transgelin 2 [Source:MGI Symbol;Acc:MGI:1312985])

HSP 1 Score: 129.028 bits (323), Expect = 1.801e-35
Identity = 78/198 (39.39%), Postives = 114/198 (57.58%), Query Frame = -1
Query:  315 MSNRIGDMGMDVQINRKLNSKYDPESEKEVIDWLNKVSGEHVAL---GMQSVQQTLKNGQILIKLINKIYEGTDKKPAAALKIRCPLKFNTMSAPFKQMENIQLFLKASEAYGVPRASLFQTVDLYESRNMAQVLNTLLQL-GTECQRNE--FKG-PTCGPKPTYENKREFTEEQLRSSQGIIGLQAGSNKGASQAGM 887
            M+NR    G+  ++ +K+  +YD + E+ +I W+     E V     G ++ Q+ LK+G +L KLIN +Y      P     ++   K    S  FKQME I  FL+A+E YG+    +FQTVDL+E +NMA V  TL+ L G    R++  F G P   PK + EN R F++ QL+  + +IGLQ G+N+GASQAGM
Sbjct:    1 MANRGPSYGLSREVQQKIEKQYDADLEQILIQWITTQCREDVGQPQPGRENFQKWLKDGTVLCKLINSLY------PEGQAPVK---KIQASSMAFKQMEQISQFLQAAERYGINTTDIFQTVDLWEGKNMACVQRTLMNLGGLAVARDDGLFSGDPNWFPKKSKENPRNFSDNQLQEGKNVIGLQMGTNRGASQAGM 189          
BLAST of SMED30029432 vs. Ensembl Mouse
Match: Cnn3 (calponin 3, acidic [Source:MGI Symbol;Acc:MGI:1919244])

HSP 1 Score: 121.324 bits (303), Expect = 4.147e-31
Identity = 89/244 (36.48%), Postives = 136/244 (55.74%), Query Frame = -1
Query:  159 NRIGDMGMDVQINRKLNSKYDPESEKEVIDWLNKVSGEHVALGMQSVQQTLKNGQILIKLINKIYEGTDKKPAAALKIRCPLKFNTMSAPFKQMENIQLFLKASEAYGVPRASLFQTVDLYESRNMAQVLNTLLQLGTECQRNEFKGPT-CGPKPTYENKREFTEEQLRSSQGIIGLQAGSNKGASQAGMR-IGTQRFINDVTTGVEMSDEGKTTIGLQMGPDKSKVDSQSGM-SFGAQRHITD 881
            N+    G+  ++  K+ SKYD ++E+++ +W+ +V+G  + +G  + Q  LK+G IL +LINK+  G+ KK             N  S  + Q+ENI  F+KA +AYG+    +F+  DL+E+ NM QV  TL+ L    +   F      G K   +  R F E +L++ Q +IGLQ G+NK ASQAGM   GT+R + D     +   + +TTI LQMG +K    SQ+GM + G +R I D
Sbjct:    5 NKGPSYGLSAEVKNKIASKYDQQAEEDLRNWIEEVTG--LGIGT-NFQLGLKDGIILCELINKLQPGSVKK------------VNESSLNWPQLENIGNFIKAIQAYGMKPHDIFEANDLFENGNMTQVQTTLVALAGLAKTKGFHTTIDIGVKYAEKQTRRFDEGKLKAGQSVIGLQMGTNKCASQAGMTAYGTRRHLYDPKMQTDKPFD-QTTISLQMGTNKGA--SQAGMLAPGTRRDIYD 230          
BLAST of SMED30029432 vs. UniProt/SwissProt
Match: sp|Q24799|MYPH_ECHGR (Myophilin OS=Echinococcus granulosus OX=6210 PE=2 SV=1)

HSP 1 Score: 164.851 bits (416), Expect = 1.711e-48
Identity = 92/202 (45.54%), Postives = 121/202 (59.90%), Query Frame = -1
Query:  285 MSNRIGDMGMDVQINRKLNSKYDPESEKEVIDWLNKVSGEHVALGMQSVQQTLKNGQILIKLINKIYEGTDKKPAAALKIRCPLKFNTMSAPFKQMENIQLFLKASEAYGVPRASLFQTVDLYESRNMAQVLNTLLQLGTECQRN-EFKGPTCGPKPTYENKREFTEEQLRSSQGIIGLQAGSNKGASQAGMRIGTQRFIND 887
            MSN     G+  Q+ +KL  K D + E E ++W+  ++G  +    +  +  LK+G +L KL+N I      KP    KI         + PFK MENI  FL+A + YGVP A LFQTVDL+E +++AQV  TL  LG  CQ + E+ GP  GPK   ENKREFTE+QLR  Q ++ LQ GSNKGASQAG+ +G QR I D
Sbjct:    1 MSNVPPPSGLSYQVKKKLEGKRDKDQENEALEWIEALTGLKLDRS-KLYEDILKDGTVLCKLMNSI------KPGCIKKIN-----ENATMPFKIMENISAFLEAMKGYGVPVADLFQTVDLFEKKDIAQVTRTLFALGRTCQTHPEYSGPVLGPKLATENKREFTEQQLREGQNVVSLQYGSNKGASQAGINMGKQRMIMD 190          
BLAST of SMED30029432 vs. UniProt/SwissProt
Match: sp|P14318|MP20_DROME (Muscle-specific protein 20 OS=Drosophila melanogaster OX=7227 GN=Mp20 PE=2 SV=2)

HSP 1 Score: 156.377 bits (394), Expect = 2.684e-45
Identity = 88/193 (45.60%), Postives = 118/193 (61.14%), Query Frame = -1
Query:  291 MGMDVQINRKLNSKYDPESEKEVIDWLNKVSGEHVALGMQSVQQTLKNGQILIKLINKIYEGTDKKPAAALKIRCPLKFNTMSAPFKQMENIQLFLKASEAYGVPRASLFQTVDLYESRNMAQVLNTLLQLGTECQRN-EFKGPTCGPKPTYENKREFTEEQLRSSQGIIGLQAGSNKGASQAGMRIGTQRFI 866
            M ++  +  K+ SK +PE +KE  +W+  +  E    G QS +  LK+GQ+L KLIN +       P A  K+      N+    FK MENI  F KA + YGVP   +FQTVDLYE +++A V NT+  LG    ++ +FKGP  GPKP  E KR+FTEEQL++ Q I+GLQAGSNKGA+QAG  +G  R I
Sbjct:    1 MSLERAVRAKIASKRNPEMDKEAQEWIEAIIAEKFPAG-QSYEDVLKDGQVLCKLINVL------SPNAVPKV------NSSGGQFKFMENINNFQKALKEYGVPDIDVFQTVDLYEKKDIANVTNTIFALGRATYKHADFKGPFLGPKPADECKRDFTEEQLKAGQTIVGLQAGSNKGATQAGQNLGAGRKI 180          
BLAST of SMED30029432 vs. UniProt/SwissProt
Match: sp|P19966|TAGL_CHICK (Transgelin OS=Gallus gallus OX=9031 GN=TAGLN PE=1 SV=3)

HSP 1 Score: 135.576 bits (340), Expect = 5.502e-37
Identity = 85/206 (41.26%), Postives = 117/206 (56.80%), Query Frame = -1
Query:  291 MSNRIGDMGMDVQINRKLNSKYDPESEKEVIDWLNKVSGEHVAL---GMQSVQQTLKNGQILIKLINKIYEGTDKKPAAALKIRCPLKFNTMSAPFKQMENIQLFLKASEAYGVPRASLFQTVDLYESRNMAQVLNTLLQLGT-ECQRNE--FKG-PTCGPKPTYENKREFTEEQLRSSQGIIGLQAGSNKGASQAGMRIGTQRFI 887
            M+N+    GM   +  K+  KYD E E  +++W+    G  V     G    Q  LKNG +L +L+N +Y      P  +  ++ P    TM   FKQME I  FLKA+E YGV +  +FQTVDL+E+++MA V  TL+ LG+    +N+  + G P    K   E+KREF+E QL+  + IIGLQ G+NKGASQAGM  G  R I
Sbjct:    1 MANKGPAYGMSRDVQSKIEKKYDDELEDRLVEWIVAQCGSSVGRPDRGRLGFQVWLKNGIVLSQLVNSLY------PDGSKPVKIPDSPPTMV--FKQMEQIAQFLKAAEDYGVVKTDMFQTVDLFEAKDMAAVQRTLVALGSLAVTKNDGHYHGDPNWFMKKAQEHKREFSESQLKEGKNIIGLQMGTNKGASQAGMSYGRPRQI 198          
BLAST of SMED30029432 vs. UniProt/SwissProt
Match: sp|Q9R1Q8|TAGL3_MOUSE (Transgelin-3 OS=Mus musculus OX=10090 GN=Tagln3 PE=1 SV=1)

HSP 1 Score: 135.191 bits (339), Expect = 6.896e-37
Identity = 82/202 (40.59%), Postives = 111/202 (54.95%), Query Frame = -1
Query:  315 MSNRIGDMGMDVQINRKLNSKYDPESEKEVIDWLNKVSGE---HVALGMQSVQQTLKNGQILIKLINKIYE-GTDKKPAAALKIRCPLKFNTMSAPFKQMENIQLFLKASEAYGVPRASLFQTVDLYESRNMAQVLNTLLQLGTE-------CQRNEFKGPTCGPKPTYENKREFTEEQLRSSQGIIGLQAGSNKGASQAGM 887
            M+NR    G+  ++  K+  KYD + E +++DW+     E   H   G    Q+ L +G +L KLIN +Y  G +  P          K +     FKQME I  FLKA+E YGV    +FQTVDL+E ++MA V  TL+ LG+        C R E   P+   +   +N+R F+EEQLR  Q +IGLQ GSNKGASQAGM
Sbjct:    1 MANRGPSYGLSREVQEKIEQKYDADLENKLVDWIILQCAEDIEHPPPGRAHFQKWLMDGTVLCKLINSLYPPGQEPIP----------KISESKMAFKQMEQISQFLKAAEVYGVRTTDIFQTVDLWEGKDMAAVQRTLMALGSVAVTKDDGCYRGE---PSWFHRKAQQNRRGFSEEQLRQGQNVIGLQMGSNKGASQAGM 189          
BLAST of SMED30029432 vs. UniProt/SwissProt
Match: sp|P37805|TAGL3_RAT (Transgelin-3 OS=Rattus norvegicus OX=10116 GN=Tagln3 PE=1 SV=2)

HSP 1 Score: 135.191 bits (339), Expect = 7.118e-37
Identity = 82/202 (40.59%), Postives = 111/202 (54.95%), Query Frame = -1
Query:  315 MSNRIGDMGMDVQINRKLNSKYDPESEKEVIDWLNKVSGE---HVALGMQSVQQTLKNGQILIKLINKIYE-GTDKKPAAALKIRCPLKFNTMSAPFKQMENIQLFLKASEAYGVPRASLFQTVDLYESRNMAQVLNTLLQLGTE-------CQRNEFKGPTCGPKPTYENKREFTEEQLRSSQGIIGLQAGSNKGASQAGM 887
            M+NR    G+  ++  K+  KYD + E +++DW+     E   H   G    Q+ L +G +L KLIN +Y  G +  P          K +     FKQME I  FLKA+E YGV    +FQTVDL+E ++MA V  TL+ LG+        C R E   P+   +   +N+R F+EEQLR  Q +IGLQ GSNKGASQAGM
Sbjct:    1 MANRGPSYGLSREVQEKIEQKYDADLENKLVDWIILQCAEDIEHPPPGRTHFQKWLMDGTVLCKLINSLYPPGQEPIP----------KISESKMAFKQMEQISQFLKAAEVYGVRTTDIFQTVDLWEGKDMAAVQRTLMALGSVAVTKDDGCYRGE---PSWFHRKAQQNRRGFSEEQLRQGQNVIGLQMGSNKGASQAGM 189          
BLAST of SMED30029432 vs. TrEMBL
Match: A0A0X3NLR9 (Transgelin OS=Schistocephalus solidus OX=70667 GN=MYPH PE=3 SV=1)

HSP 1 Score: 359.377 bits (921), Expect = 1.379e-119
Identity = 171/243 (70.37%), Postives = 195/243 (80.25%), Query Frame = -1
Query:  159 MSNRIGDMGMDVQINRKLNSKYDPESEKEVIDWLNKVSGEHVALGMQSVQQTLKNGQILIKLINKIYEGTDKKPAAALKIRCPLKFNTMSAPFKQMENIQLFLKASEAYGVPRASLFQTVDLYESRNMAQVLNTLLQLGTECQRNEFKGPTCGPKPTYENKREFTEEQLRSSQGIIGLQAGSNKGASQAGMRIGTQRFINDVTTGVEMSDEGKTTIGLQMGPDKSKVDSQSGMSFGAQRHITD 887
            M+NR+GDMGMDVQ+NR++NSKYDPE+EKEVIDW+NK++GE V LG ++V   LKNG ILIKLIN +Y GT + P AA + + P KFNTM+APFKQMENIQ+FL A+EAYGVPRASLFQTVDLYE RNM QVLN LLQLGTECQRN F GP CGPKPTYENKREFT EQL +S+ IIGLQAGSNKGASQ+GM +G  R I D+    EMS E K  IGLQ G +K    SQSGMS GA RHI D
Sbjct:    1 MANRMGDMGMDVQVNRRMNSKYDPETEKEVIDWMNKITGESVPLGRENVAAALKNGHILIKLINAVYAGTSQLPPAAQRKKKPFKFNTMTAPFKQMENIQIFLTAAEAYGVPRASLFQTVDLYELRNMTQVLNCLLQLGTECQRNNFNGPVCGPKPTYENKREFTAEQLNASKTIIGLQAGSNKGASQSGMSMGATRHIADIKVD-EMSAEAKGVIGLQSGSNKGA--SQSGMSMGATRHIAD 240          

HSP 2 Score: 69.3218 bits (168), Expect = 2.144e-9
Identity = 38/68 (55.88%), Postives = 47/68 (69.12%), Query Frame = -1
Query:  159 IGLQAGSNKGASQAGMRIGTQRFINDVTTGVEMSDEGKTTIGLQMGPDKSKVDSQSGMSFGAQRHITD 362
            IGL +G+NKGASQ+GM +G  R I D   G EMS EGK+TIGLQ G ++    +QSGM+ G  RHI D
Sbjct:  254 IGLLSGTNKGASQSGMSMGAVRHIADTRVG-EMSAEGKSTIGLQAGSNQGA--TQSGMTMGGVRHIAD 318          

HSP 3 Score: 69.3218 bits (168), Expect = 2.287e-9
Identity = 38/68 (55.88%), Postives = 48/68 (70.59%), Query Frame = -1
Query:  159 IGLQAGSNKGASQAGMRIGTQRFINDVTTGVEMSDEGKTTIGLQMGPDKSKVDSQSGMSFGAQRHITD 362
            IGLQAGSN+GA+Q+GM +G  R I D+    +MS EG + IGLQ G +     SQ+GM+FGAQRHI D
Sbjct:  293 IGLQAGSNQGATQSGMTMGGVRHIADIKV-EDMSKEGASVIGLQAGSNLGA--SQAGMNFGAQRHIGD 357          
BLAST of SMED30029432 vs. TrEMBL
Match: K4IR16 (Calponin (Fragment) OS=Solen grandis OX=165599 PE=2 SV=1)

HSP 1 Score: 342.043 bits (876), Expect = 1.707e-114
Identity = 164/243 (67.49%), Postives = 194/243 (79.84%), Query Frame = -1
Query:  159 MSNRIGDMGMDVQINRKLNSKYDPESEKEVIDWLNKVSGEHVALGMQSVQQTLKNGQILIKLINKIYEGTDKKPAAALKIRCPLKFNTMSAPFKQMENIQLFLKASEAYGVPRASLFQTVDLYESRNMAQVLNTLLQLGTECQRNEFKGPTCGPKPTYENKREFTEEQLRSSQGIIGLQAGSNKGASQAGMRIGTQRFINDVTTGVEMSDEGKTTIGLQMGPDKSKVDSQSGMSFGAQRHITD 887
            MSNR GDMGMDVQ+NR+LNS+YD ++EKEV+ W+N+++GE+V LG ++V   LKNGQILIKL+NK+YEGT+  P    K + P K NTMSAPFKQMENIQ+FL A+EAYGVPR SLFQTVDL+E RNMAQVL+ LLQLGTECQRN F GPTCGPKPTYE KREFTE+QL +S+ +IGLQAGSNKGASQ GM IG  R I D+    +MS EG+  IGLQ G +K    SQSGM+ GA RHI D
Sbjct:    1 MSNRAGDMGMDVQVNRRLNSRYDADTEKEVVQWINQLTGENVPLGRENVAAALKNGQILIKLVNKVYEGTNPLPPTVTKKKHPFKANTMSAPFKQMENIQIFLTAAEAYGVPRTSLFQTVDLFEMRNMAQVLSCLLQLGTECQRNNFNGPTCGPKPTYETKREFTEDQLAASKTVIGLQAGSNKGASQKGMSIGAVRHIADIKAD-DMSKEGQGIIGLQAGSNKGA--SQSGMAMGAVRHIAD 240          
BLAST of SMED30029432 vs. TrEMBL
Match: A0A068YJ88 (Transgelin OS=Echinococcus multilocularis OX=6211 GN=EmuJ_001004900 PE=3 SV=1)

HSP 1 Score: 349.747 bits (896), Expect = 3.401e-114
Identity = 167/243 (68.72%), Postives = 195/243 (80.25%), Query Frame = -1
Query:  159 MSNRIGDMGMDVQINRKLNSKYDPESEKEVIDWLNKVSGEHVALGMQSVQQTLKNGQILIKLINKIYEGTDKKPAAALKIRCPLKFNTMSAPFKQMENIQLFLKASEAYGVPRASLFQTVDLYESRNMAQVLNTLLQLGTECQRNEFKGPTCGPKPTYENKREFTEEQLRSSQGIIGLQAGSNKGASQAGMRIGTQRFINDVTTGVEMSDEGKTTIGLQMGPDKSKVDSQSGMSFGAQRHITD 887
            MSNR GDMGMDVQ+NR+LNS+YD E+EKEV+ W+N+++GE+V LG ++V   LKNGQILIKL+NK+YEGT+  P    K + P K NTMSAPFKQMENIQ+FL A+EAYGVPR SLFQTVDL+E RNMAQVL+ LLQLGTECQRN F GPTCGPKPTYENKREFTE+QL +S+ IIGLQAGSNKGASQ GM IG  R I D+    +MS EG+  IGLQ G +K    SQSGM+ GA RHI D
Sbjct:   27 MSNRAGDMGMDVQVNRRLNSRYDAETEKEVVQWINQLTGENVPLGRENVAAALKNGQILIKLVNKVYEGTNPLPPTVTKKKHPFKANTMSAPFKQMENIQIFLTAAEAYGVPRTSLFQTVDLFEMRNMAQVLSCLLQLGTECQRNNFNGPTCGPKPTYENKREFTEDQLAASKTIIGLQAGSNKGASQKGMSIGAVRHIADIKAD-DMSKEGQGIIGLQAGSNKGA--SQSGMAMGAVRHIAD 266          

HSP 2 Score: 75.8702 bits (185), Expect = 2.625e-11
Identity = 41/74 (55.41%), Postives = 51/74 (68.92%), Query Frame = -1
Query:  159 RSSQGIIGLQAGSNKGASQAGMRIGTQRFINDVTTGVEMSDEGKTTIGLQMGPDKSKVDSQSGMSFGAQRHITD 380
            +  Q +IGLQAGSN GASQ+GM +GT R I D+    EMS EG+  IGLQ G +  K  +QSGMS GA+RH+ D
Sbjct:  352 KEGQSVIGLQAGSNLGASQSGMSMGTMRHITDIKVD-EMSKEGQGVIGLQAGTN--KFANQSGMSMGARRHVAD 422          

HSP 3 Score: 75.485 bits (184), Expect = 2.876e-11
Identity = 39/79 (49.37%), Postives = 55/79 (69.62%), Query Frame = -1
Query:  159 TEEQLRSSQGIIGLQAGSNKGASQAGMRIGTQRFINDVTTGVEMSDEGKTTIGLQMGPDKSKVDSQSGMSFGAQRHITD 395
             +E  +  QG+IGLQAG+NK A+Q+GM +G +R + D+  G +MS +    +GLQ G ++    SQ+GMSFGAQRHITD
Sbjct:  386 VDEMSKEGQGVIGLQAGTNKFANQSGMSMGARRHVADIKVG-DMSKDSAGILGLQSGSNQG--SSQAGMSFGAQRHITD 461          
BLAST of SMED30029432 vs. TrEMBL
Match: A0A5J4NR98 (Calponin OS=Paragonimus westermani OX=34504 GN=DEA37_0008826 PE=3 SV=1)

HSP 1 Score: 344.354 bits (882), Expect = 8.819e-114
Identity = 168/243 (69.14%), Postives = 194/243 (79.84%), Query Frame = -1
Query:  159 MSNRIGDMGMDVQINRKLNSKYDPESEKEVIDWLNKVSGEHVALGMQSVQQTLKNGQILIKLINKIYEGTDKKPAAALKIRCPLKFNTMSAPFKQMENIQLFLKASEAYGVPRASLFQTVDLYESRNMAQVLNTLLQLGTECQRNEFKGPTCGPKPTYENKREFTEEQLRSSQGIIGLQAGSNKGASQAGMRIGTQRFINDVTTGVEMSDEGKTTIGLQMGPDKSKVDSQSGMSFGAQRHITD 887
            M+NR+ DMGMDVQINR+LNSKYDPESE E I WLNK++GE+V LG ++V  TLKNGQILIKLIN +Y+GT   P  A +++ P K NTM+APFKQMENIQ FL A+ AYGVPRASLFQTVDLYE+RNMAQVLNT+LQLGTECQRN F G TCGPKPT+ENKREFTEEQLR+S+GIIGLQAG+NK ASQAGM  G  R I D+       D  +  IGLQMG +  K+ SQ+GMS G  RHITD
Sbjct:    1 MANRMVDMGMDVQINRRLNSKYDPESESEAIGWLNKLTGENVPLGRENVCATLKNGQILIKLINAVYDGTASLPPEAARMKRPFKANTMTAPFKQMENIQTFLNAAGAYGVPRASLFQTVDLYEARNMAQVLNTILQLGTECQRNNFNGETCGPKPTFENKREFTEEQLRASEGIIGLQAGTNKLASQAGMSHGGPRHITDIKVDAASKDS-QGVIGLQMGTN--KLASQAGMSHGGPRHITD 240          

HSP 2 Score: 98.9821 bits (245), Expect = 1.326e-19
Identity = 67/151 (44.37%), Postives = 87/151 (57.62%), Query Frame = -1
Query:  153 QMENIQLFLKASEAYGVPRASLFQTVDLYESRNMAQVLNTLLQLGTECQRNEFKGPTCGPKPTYENKREFTEEQLRSSQGIIGLQAGSNKGASQAGMRIGTQRFINDVTTGVEMSDEGKTTIGLQMGPDKSKVDSQSGMSFGAQRHITDSY 605
            QM   +L  +A  ++G PR      VD   S+    V+   LQ+G+    ++      GP+   + K +   ++    QG+IGLQ GSNKGASQAGM  G QR I D+  G EMS EG   IG+QMG  K +V SQSGMSFGAQRHI DS+
Sbjct:  218 QMGTNKLASQAGMSHGGPRHITDIKVD-AASKESQSVIG--LQMGSNKGASQAGMSHGGPRHITDIKVDAASKE---GQGVIGLQMGSNKGASQAGMSHGGQRHIADIKVG-EMSKEGAGVIGMQMGLGKDQVASQSGMSFGAQRHILDSH 361          
BLAST of SMED30029432 vs. TrEMBL
Match: W6V0C9 (Transgelin OS=Echinococcus granulosus OX=6210 GN=EGR_00866 PE=3 SV=1)

HSP 1 Score: 347.436 bits (890), Expect = 4.089e-113
Identity = 165/243 (67.90%), Postives = 195/243 (80.25%), Query Frame = -1
Query:  159 MSNRIGDMGMDVQINRKLNSKYDPESEKEVIDWLNKVSGEHVALGMQSVQQTLKNGQILIKLINKIYEGTDKKPAAALKIRCPLKFNTMSAPFKQMENIQLFLKASEAYGVPRASLFQTVDLYESRNMAQVLNTLLQLGTECQRNEFKGPTCGPKPTYENKREFTEEQLRSSQGIIGLQAGSNKGASQAGMRIGTQRFINDVTTGVEMSDEGKTTIGLQMGPDKSKVDSQSGMSFGAQRHITD 887
            MSNR GDMGMDVQ+NR+LNS+YD ++EKEV+ W+N+++GE+V LG ++V   LKNGQILIKL+NK+YEGT+  P    K + P K NTMSAPFKQMENIQ+FL A+EAYGVPR SLFQTVDL+E RNMAQVL+ LLQLGTECQRN F GPTCGPKPTYENKREFTE+QL +S+ +IGLQAGSNKGASQ GM IG  R I D+    +MS EG+  IGLQ G +K    SQSGM+ GA RHI D
Sbjct:   53 MSNRAGDMGMDVQVNRRLNSRYDADTEKEVVQWINQLTGENVPLGRENVAAALKNGQILIKLVNKVYEGTNPLPPTVTKKKHPFKANTMSAPFKQMENIQIFLTAAEAYGVPRTSLFQTVDLFEMRNMAQVLSCLLQLGTECQRNNFNGPTCGPKPTYENKREFTEDQLAASKTVIGLQAGSNKGASQKGMSIGAVRHIADIKAD-DMSKEGQGIIGLQAGSNKGA--SQSGMAMGAVRHIAD 292          

HSP 2 Score: 75.0998 bits (183), Expect = 4.361e-11
Identity = 39/79 (49.37%), Postives = 55/79 (69.62%), Query Frame = -1
Query:  159 TEEQLRSSQGIIGLQAGSNKGASQAGMRIGTQRFINDVTTGVEMSDEGKTTIGLQMGPDKSKVDSQSGMSFGAQRHITD 395
             +E  +  QG+IGLQAG+N+ A+Q+GM +G +R + D+  G +MS +    IGLQ G ++    SQ+GMSFGAQRHITD
Sbjct:  412 VDEMSKEGQGVIGLQAGTNQFANQSGMSMGARRHVADIKVG-DMSKDSAGIIGLQSGSNQG--SSQAGMSFGAQRHITD 487          

HSP 3 Score: 72.4034 bits (176), Expect = 3.569e-10
Identity = 39/74 (52.70%), Postives = 50/74 (67.57%), Query Frame = -1
Query:  159 RSSQGIIGLQAGSNKGASQAGMRIGTQRFINDVTTGVEMSDEGKTTIGLQMGPDKSKVDSQSGMSFGAQRHITD 380
            +  Q +IGLQAGSN GASQ+GM +G  R I D+    EMS EG+  IGLQ G +  +  +QSGMS GA+RH+ D
Sbjct:  378 KEGQSVIGLQAGSNLGASQSGMSMGAMRHITDIKVD-EMSKEGQGVIGLQAGTN--QFANQSGMSMGARRHVAD 448          
BLAST of SMED30029432 vs. Ensembl Cavefish
Match: tagln (transgelin-like [Source:NCBI gene;Acc:103045968])

HSP 1 Score: 139.428 bits (350), Expect = 2.529e-39
Identity = 83/201 (41.29%), Postives = 122/201 (60.70%), Query Frame = -1
Query:  315 NMSNRIGDMGMDVQINRKLNSKYDPESEKEVIDWLNKVSGEHVAL---GMQSVQQTLKNGQILIKLINKIYEGTDKKPAAALKIRCPLKFNTMSAP--FKQMENIQLFLKASEAYGVPRASLFQTVDLYESRNMAQVLNTLLQLGTEC---QRNEFKG-PTCGPKPTYENKREFTEEQLRSSQGIIGLQAGSNKGASQAGM 890
            +M+N+    G+  Q+  K++ KYDPE E  +++W+    G  VA    G Q  Q  LK+G +L +LIN +++ +DK          P+K    S+P  FKQME +  FL A+EAYGV +  +FQTVDL+E +++A V  TL+ LG+         F+G P    K   EN+R+F+++QL+S + +IGLQ GSNKGASQAGM
Sbjct:    8 SMANKGPSYGLSRQVQDKIDRKYDPELEARLVEWIVAQCGSGVAKPAEGKQGFQDWLKDGCVLSELINSLHD-SDK----------PVK-KIQSSPMAFKQMEQVSQFLNAAEAYGVTKTDMFQTVDLWEGKDLAAVQRTLMALGSIAVTKDDGHFRGNPDWFFKKAQENRRDFSDDQLKSGKHVIGLQMGSNKGASQAGM 196          
BLAST of SMED30029432 vs. Ensembl Cavefish
Match: tagln3a (transgelin-3-like [Source:NCBI gene;Acc:103024278])

HSP 1 Score: 135.961 bits (341), Expect = 5.594e-38
Identity = 80/199 (40.20%), Postives = 114/199 (57.29%), Query Frame = -1
Query:  315 MSNRIGDMGMDVQINRKLNSKYDPESEKEVIDWLNKVSGEHVAL---GMQSVQQTLKNGQILIKLINKIYEGTDKKPAAALKIRCPLKFNTMSAPFKQMENIQLFLKASEAYGVPRASLFQTVDLYESRNMAQVLNTLLQLGTECQRNEFKGPTCGPKPTYENK-----REFTEEQLRSSQGIIGLQAGSNKGASQAGM 887
            M+NR    G+  ++  K+  KYDP+ E  ++DW+    G+ +     G Q+ Q  L +G IL +LIN +Y      P+ +  I+   K       FKQME I  FL+A+E YGV R  +FQTVDL+E +++A V  TL+ LG+E    +  G   G K  +  K     REF+EEQLR  + +IGLQ GSN+GASQ+GM
Sbjct:    1 MANRGPSYGLSREVQEKIEQKYDPDLESRLVDWIMLQCGDDLVRPEPGKQNFQTWLMDGTILCRLINSLY------PSGSEPIK---KIPETKMAFKQMEKISQFLEAAEVYGVVRGDIFQTVDLWEGKDLAAVQRTLMALGSEALTKD-DGHYHGNKDWFRRKAKGHRREFSEEQLRQGRALIGLQMGSNRGASQSGM 189          
BLAST of SMED30029432 vs. Ensembl Cavefish
Match: TAGLN3 (transgelin 3 [Source:NCBI gene;Acc:103030448])

HSP 1 Score: 128.257 bits (321), Expect = 2.806e-35
Identity = 81/199 (40.70%), Postives = 109/199 (54.77%), Query Frame = -1
Query:  315 MSNRIGDMGMDVQINRKLNSKYDPESEKEVIDWLNKVSG---EHVALGMQSVQQTLKNGQILIKLINKIYEGTDKKPAAALKIRCPLKFNTMSAPFKQMENIQLFLKASEAYGVPRASLFQTVDLYESRNMAQVLNTLLQLGTECQRNEFKGPTCGPKPTYE-----NKREFTEEQLRSSQGIIGLQAGSNKGASQAGM 887
            M+NR    G+  ++  K+  KYD + E  ++DW+    G   E    G Q+ Q  L +G IL +LIN +Y      P     I+   K       FKQME I  FL A+EAYGV    +FQTVDL+E ++MA V  TL+ LG+     +  G   G +  +      N+REF+EEQLR  Q +IGLQ GSN+GASQAGM
Sbjct:    1 MANRGPSYGLSREVLEKIEQKYDTDLEVRLVDWIIAQCGGNLERPLPGKQNFQNWLMDGTILCRLINSLY------PRGKEPIK---KIQETQMAFKQMEKISQFLVAAEAYGVITTDIFQTVDLWEGKDMAAVQRTLMALGSVALTKD-DGHYRGNRDWFHRTAQSNRREFSEEQLRQGQNLIGLQMGSNRGASQAGM 189          
BLAST of SMED30029432 vs. Ensembl Cavefish
Match: cnn1b (calponin 1 [Source:NCBI gene;Acc:103040806])

HSP 1 Score: 116.701 bits (291), Expect = 7.682e-30
Identity = 83/239 (34.73%), Postives = 128/239 (53.56%), Query Frame = -1
Query:  159 MGMDVQINRKLNSKYDPESEKEVIDWLNKVSGEHVALGMQSVQQTLKNGQILIKLINKIYEGTDKKPAAALKIRCPLKFNTMSAPFKQMENIQLFLKASEAYGVPRASLFQTVDLYESRNMAQVLNTLLQLGTECQRNEFKGP-TCGPKPTYENKREFTEEQLRSSQGIIGLQAGSNKGASQAGM-RIGTQRFINDVTTGVEMSDEGKTTIGLQMGPDKSKVDSQSGMSF-GAQRHITD 866
             G+  ++  KL  KYDP+ E+E+  W+N+V+G  V     +  + LK+G  L +LIN +  GT +K             N  S  + Q+ENI  F++  + YG+    +F+  DL+E+ N  QV +TL+ L    +    +     G K   + +R F  E+L+  + IIGLQ G+NK ASQ GM   GT+R + D   G+E   + ++TI LQMG +K    +QSGM+  G +R I D
Sbjct:   12 FGLSAEVKSKLAQKYDPQKEEELRLWINEVTGRKVP---DNFMEGLKDGVTLCELINALQPGTVRK------------INNSSQNWHQLENIGNFVRGIQEYGLRPHDIFEANDLFENVNHTQVQSTLIALAGMAKSKGLRTKYDIGVKYAEKQQRRFAPEKLKEGRNIIGLQMGTNKFASQKGMTSYGTRRHLYDPKVGMENPLD-QSTISLQMGTNKGA--NQSGMTAPGTKRQIFD 232          
BLAST of SMED30029432 vs. Ensembl Cavefish
Match: cnn3a (calponin 3 [Source:NCBI gene;Acc:103024651])

HSP 1 Score: 117.087 bits (292), Expect = 1.136e-29
Identity = 85/238 (35.71%), Postives = 131/238 (55.04%), Query Frame = -1
Query:  159 GMDVQINRKLNSKYDPESEKEVIDWLNKVSGEHVALGMQSVQQTLKNGQILIKLINKIYEGTDKKPAAALKIRCPLKFNTMSAPFKQMENIQLFLKASEAYGVPRASLFQTVDLYESRNMAQVLNTLLQLGTECQRNEFKGPT-CGPKPTYENKREFTEEQLRSSQGIIGLQAGSNKGASQAGMR-IGTQRFINDVTTGVEMSDEGKTTIGLQMGPDKSKVDSQSGMSF-GAQRHITD 863
            G+  ++  K+  KYD + E+E+  W+ +V+G  +     + Q+ LK+G IL +LINK+      +P A  KI      N     + ++EN+  F+KA   YG+    +F+  DL+E+ NM QV  TLL L +  +          G K   + +R F +E++++ Q +IGLQ G+NK ASQAGM   GT+R + D  T  +   + +TTI LQMG +K    SQ+GMS  G +R I D
Sbjct:   11 GLSAEVKSKIAQKYDLQKEEELRFWIEEVTGMPIG---DNFQKGLKDGVILCELINKL------QPGAIKKI------NHSQLNWHKLENLGNFIKAILTYGLKPNDIFEANDLFENGNMTQVQTTLLALASMAKTKGMDSKVDIGVKYADKQERHFDDEKMKAGQCVIGLQMGTNKCASQAGMTAYGTRRHLYDPKTQTDKPYD-QTTISLQMGTNKGA--SQAGMSAPGTRRDIFD 230          
BLAST of SMED30029432 vs. Ensembl Yeast
Match: SCP1 (Component of yeast cortical actin cytoskeleton; binds and cross links actin filaments; originally identified by its homology to calponin (contains a calponin-like repeat) but the Scp1p domain structure is more similar to transgelin [Source:SGD;Acc:S000005894])

HSP 1 Score: 77.7962 bits (190), Expect = 1.979e-17
Identity = 60/197 (30.46%), Postives = 97/197 (49.24%), Query Frame = -1
Query:  291 GMDVQINRKLNSKYDPESEKEVIDWLNKVSGEHVALGMQSVQQTLKNGQILIKLINKIYEGTDKKPAAALKIRCPLKFNTMSAPFKQMENIQLFLKASEAYGVPRASLFQTVDLYESRNMAQVLNTLLQLGTEC-QRNEFKGPTCGPKPTYENKREFTE---EQLRSSQGIIGLQAGSNKGASQA--GMRIGTQRFI 863
             +D  + +   SK+ PE+ + +  W+ K   + +A     + + LK+G +L KL N +YE  D   A  +       + +   PF QM+ I  FL  S  YGVP   LFQT+DL+E ++ A V  TL  L     +++  + P  GP+ + +  R   +   + L+   G    + G  KGASQA  G+ +G +R I
Sbjct:   11 SLDEDLRQLRESKFSPEAIQNIKIWVYKSVLKEIA-PPGDLLECLKDGTVLCKLANILYEA-DTGEANHIS------WKSSKMPFVQMDQISQFLSFSRKYGVPEDELFQTIDLFEKKDPAIVFQTLKSLSRYANKKHTDRFPVLGPQLSTKKPRPPVKSKPKHLQDGTGWSTFEYGYMKGASQATEGVVLGQRRDI 199          
BLAST of SMED30029432 vs. Ensembl Nematostella
Match: EDO30656 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T059])

HSP 1 Score: 144.436 bits (363), Expect = 3.692e-42
Identity = 79/180 (43.89%), Postives = 104/180 (57.78%), Query Frame = -1
Query:  297 LNSKYDPESEKEVIDWLNKVSGEHVALGMQSVQQTLKNGQILIKLINKIYEGTDKKPAAALKIRCPLKFNTMSAPFKQMENIQLFLKASEAYGVPRASLFQTVDLYESRNMAQVLNTLLQLGTECQRNEFKGPTCGPKPTYENKREFTEEQLRSSQGIIGLQAGSNKGASQAGMRIGTQR 836
            +++KYDP  + E +DW+ KV GE V  G    +  LK+GQIL +L N +  G D +           K N     FK MENI  FL     +GV ++ LFQTVDLYE +N+ QV+N++  L  +        P  GPK + +N REFTEEQ R+ +G+IGLQ GSNKGASQAG   G  R
Sbjct:    1 MSAKYDPVMDAEAVDWIEKVLGEEVCQGKSINEDVLKDGQILCRLANHL--GGDIQ-----------KINNSKMAFKMMENIAKFLDFCGTFGVAKSDLFQTVDLYEKQNIQQVINSIHALARKANSKGLSVPILGPKESQKNPREFTEEQKRAGEGVIGLQMGSNKGASQAGDTFGRPR 167          
BLAST of SMED30029432 vs. Ensembl Nematostella
Match: EDO46712 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RPC0])

HSP 1 Score: 140.198 bits (352), Expect = 2.731e-40
Identity = 82/203 (40.39%), Postives = 111/203 (54.68%), Query Frame = -1
Query:  297 MSNRIGDMGMDVQINRKLNSKYDPESEKEVIDWLNKVSGEHV---ALGMQSVQQTLKNGQILIKLINKIYEGTDKKPAAALKIRCPLKFNTMSAPFKQMENIQLFLKASEAYGVPRASLFQTVDLYESRNMAQVLNTLLQLGTECQRNEFKGPTC---GPKPTYENKREFTEEQLRSSQGIIGLQAGSNKGASQAGMRIGTQR 887
            M++R    GM  ++ RK ++KYD +   +   W+  + GE V     G   V + LK+GQ+L ++ NK+  G +            +K N+    FK MEN   FL+  +  GVP+  +FQTVDLYE +NM  V+N +  LG +       G TC   GPK    N REFTEEQ R+ QG+IGLQ GSNKGASQAG   G  R
Sbjct:    1 MASRPKGYGMTAELARKRDAKYDSDLAAQATAWIEGILGERVFGGKTGADDVHEVLKDGQVLCRVANKL--GGN------------IKINSSKMAFKMMENTGKFLEFCDTIGVPKTDMFQTVDLYEKQNMPGVINGIHALGRKAHST---GKTCLALGPKEASANPREFTEEQRRAGQGVIGLQMGSNKGASQAGDHFGRPR 186          
BLAST of SMED30029432 vs. Ensembl Nematostella
Match: EDO44075 (Myophilin-like protein; Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RWR1])

HSP 1 Score: 137.117 bits (344), Expect = 4.337e-39
Identity = 84/207 (40.58%), Postives = 113/207 (54.59%), Query Frame = -1
Query:  285 MSNRIGDMGMDVQINRKLNSKYDPESEKEVIDWLNKVSGEHV---ALGMQSVQQTLKNGQILIKLINKIYEGTDKKPAAALKIRCPLKFNTMSAPFKQMENIQLFLKASEAYGVPRASLFQTVDLYESRNMAQVLNTLLQLGTECQR-NEFKGPTCGPKPTYENKREFTEEQLRSSQGIIGLQAG--SNKGASQAGMRIGTQRFIND 887
            M++R    GM    NRK+  KYDP+ E+E   W+  V GE V   A G   VQ+ LK+G+IL +L              A+K+   +K N  + PFKQMENI  FL      GV     FQT DLY++ NM  V++ +  +G +CQ   +   PT GPK    N REFTEEQLR+ + I+G+QAG   ++ ASQ+G   G  R I D
Sbjct:    1 MASRPRGYGMSAAANRKILEKYDPQQEEEARIWIEAVLGEKVFGGAEGPDDVQKVLKDGKILARL--------------AIKLGAKIKVNEQNMPFKQMENIGNFLSHCGHLGVASGDQFQTADLYDNANMTSVISCIHAVGRKCQLIKDLDIPTLGPKEAEANVREFTEEQLRAGESILGMQAGPTQDQVASQSGDHYGRGRQITD 193          
BLAST of SMED30029432 vs. Ensembl Nematostella
Match: EDO46658 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RPB8])

HSP 1 Score: 122.865 bits (307), Expect = 5.778e-34
Identity = 74/185 (40.00%), Postives = 100/185 (54.05%), Query Frame = -1
Query:  345 MSNRIGDMGMDVQINRKLNSKYDPESEKEVIDWLNKVSGEHV---ALGMQSVQQTLKNGQILIKLINKIYEGTDKKPAAALKIRCPLKFNTMSAPFKQMENIQLFLKASEA-YGVPRASLFQTVDLYESRNMAQVLNTLLQLGTECQRNEFKGPTCGPKPTYENKREFTEEQLRSSQGIIGLQAG 887
            M+N+    G   +  +K++SKYDP  E+E   W++ V GE +     G+ +V QTLK+GQ+L +L N++  G D            LK N    PFKQMENI  FL   E   GV +  LFQTVDLYE  NM  V+  +  +G          P  GPK    N R+F+EEQLR+ +GI+  QAG
Sbjct:    1 MANKPRGYGFSAEAAKKIDSKYDPTKEEEARKWIDTVLGESIFGENGGVDTVHQTLKDGQVLCRLANRL--GGD------------LKINQQKMPFKQMENIGNFLSFIENNLGVAKNDLFQTVDLYEKSNMWNVICCIHAVGRRAAAKGLDVPVLGPKEADANPRQFSEEQLRAGEGILTKQAG 171          
BLAST of SMED30029432 vs. Ensembl Nematostella
Match: EDO30654 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T056])

HSP 1 Score: 107.842 bits (268), Expect = 6.118e-29
Identity = 54/105 (51.43%), Postives = 68/105 (64.76%), Query Frame = -1
Query:  297 FKQMENIQLFLKASEAYGVPRASLFQTVDLYESRNMAQVLNTLLQLGTECQRNEFKGPTCGPKPTYENKREFTEEQLRSSQGIIGLQAGSNKGASQAGMRIGTQR 611
            FK MENI  FL     +GV ++ LFQTVDLYE +N+ QV+N++  L  +        P  GPK + +N REFTEEQ R+ +G+IGLQ GSNKGASQAG   G  R
Sbjct:    3 FKMMENIAKFLDFCGTFGVAKSDLFQTVDLYEKQNIQQVINSIYALARKANSKGLSVPILGPKESQKNPREFTEEQKRAGEGVIGLQMGSNKGASQAGDTFGRPR 107          
BLAST of SMED30029432 vs. Ensembl Medaka
Match: tagln (transgelin [Source:NCBI gene;Acc:101169057])

HSP 1 Score: 136.732 bits (343), Expect = 1.928e-38
Identity = 86/208 (41.35%), Postives = 122/208 (58.65%), Query Frame = -1
Query:  291 MSNRIGDMGMDVQINRKLNSKYDPESEKEVIDWLNKVSGEHVAL---GMQSVQQTLKNGQILIKLINKIYEGTDKKPAAALKIRCPLKFNTMSAP--FKQMENIQLFLKASEAYGVPRASLFQTVDLYESRNMAQVLNTLLQLGT-ECQRNE--FKG-PTCGPKPTYENKREFTEEQLRSSQGIIGLQAGSNKGASQAGMRIGTQRFI 887
            M+N+    GM  Q+  K++SKYDPE E+ +++W+ +  G  V     G    Q  LK+G IL +LIN ++ G             P+K  T S+P  FKQME I  FL A+E YGV +  +FQTVDL+E +++A V  TL  LG+    ++E  + G P    K   ENKR+F+++QL++ + +IGLQ GSNKGASQ GM  G  R I
Sbjct:    8 MANKGPSYGMSRQVQEKIDSKYDPELEQILVEWICRQCGSGVGRPEPGKMGFQAWLKDGCILGELINSLFAGEK-----------PVK-KTQSSPMAFKQMEQISQFLNAAEKYGVTKTDMFQTVDLWEGKDLAAVQRTLSALGSLAVTKDEGTYNGDPNWFFKKAQENKRDFSDDQLKAGKNVIGLQMGSNKGASQEGMSYGRPRQI 203          
BLAST of SMED30029432 vs. Ensembl Medaka
Match: tagln (transgelin [Source:NCBI gene;Acc:101169057])

HSP 1 Score: 136.732 bits (343), Expect = 2.085e-38
Identity = 86/208 (41.35%), Postives = 122/208 (58.65%), Query Frame = -1
Query:  291 MSNRIGDMGMDVQINRKLNSKYDPESEKEVIDWLNKVSGEHVAL---GMQSVQQTLKNGQILIKLINKIYEGTDKKPAAALKIRCPLKFNTMSAP--FKQMENIQLFLKASEAYGVPRASLFQTVDLYESRNMAQVLNTLLQLGT-ECQRNE--FKG-PTCGPKPTYENKREFTEEQLRSSQGIIGLQAGSNKGASQAGMRIGTQRFI 887
            M+N+    GM  Q+  K++SKYDPE E+ +++W+ +  G  V     G    Q  LK+G IL +LIN ++ G             P+K  T S+P  FKQME I  FL A+E YGV +  +FQTVDL+E +++A V  TL  LG+    ++E  + G P    K   ENKR+F+++QL++ + +IGLQ GSNKGASQ GM  G  R I
Sbjct:    1 MANKGPSYGMSRQVQEKIDSKYDPELEQILVEWICRQCGSGVGRPEPGKMGFQAWLKDGCILGELINSLFAGEK-----------PVK-KTQSSPMAFKQMEQISQFLNAAEKYGVTKTDMFQTVDLWEGKDLAAVQRTLSALGSLAVTKDEGTYNGDPNWFFKKAQENKRDFSDDQLKAGKNVIGLQMGSNKGASQEGMSYGRPRQI 196          
BLAST of SMED30029432 vs. Ensembl Medaka
Match: ENSORLT00000011293.2 (transgelin [Source:NCBI gene;Acc:101159525])

HSP 1 Score: 135.576 bits (340), Expect = 7.193e-38
Identity = 82/198 (41.41%), Postives = 119/198 (60.10%), Query Frame = -1
Query:  315 MSNRIGDMGMDVQINRKLNSKYDPESEKEVIDWLNKVSGEHVAL---GMQSVQQTLKNGQILIKLINKIYEGTDKKPAAALKIRCPLKFNTMSAPFKQMENIQLFLKASEAYGVPRASLFQTVDLYESRNMAQVLNTLLQLGT-ECQRNE--FKG-PTCGPKPTYENKREFTEEQLRSSQGIIGLQAGSNKGASQAGM 887
            M+NR    G+  ++  K++ KYDPE E+ ++ W+    G  V          Q+ LK+G +L +LIN +Y     KP   +K       N+  A FKQME I LFLK +EAYGV +  +FQTVDL+ES+++A V  TL+ LG+    +N+  ++G P    +   EN+R+F+EEQL   + +IGLQ G+NKGASQAGM
Sbjct:    1 MANRGPAYGLSREVQSKIDKKYDPELEERLVQWIIAQCGSGVGQPEPDKTGFQKWLKDGCVLCELINSLYGAN--KPIKTIK-------NSTMA-FKQMEQISLFLKYAEAYGVIKTDMFQTVDLFESKDLAAVQRTLMALGSLAVTKNDGNYRGDPNWFHRKAQENRRDFSEEQLSGGKSVIGLQMGTNKGASQAGM 188          
BLAST of SMED30029432 vs. Ensembl Medaka
Match: ENSORLT00000028673.1 (transgelin [Source:NCBI gene;Acc:101159525])

HSP 1 Score: 135.191 bits (339), Expect = 8.369e-38
Identity = 82/198 (41.41%), Postives = 119/198 (60.10%), Query Frame = -1
Query:  315 MSNRIGDMGMDVQINRKLNSKYDPESEKEVIDWLNKVSGEHVAL---GMQSVQQTLKNGQILIKLINKIYEGTDKKPAAALKIRCPLKFNTMSAPFKQMENIQLFLKASEAYGVPRASLFQTVDLYESRNMAQVLNTLLQLGT-ECQRNE--FKG-PTCGPKPTYENKREFTEEQLRSSQGIIGLQAGSNKGASQAGM 887
            M+NR    G+  ++  K++ KYDPE E+ ++ W+    G  V          Q+ LK+G +L +LIN +Y     KP   +K       N+  A FKQME I LFLK +EAYGV +  +FQTVDL+ES+++A V  TL+ LG+    +N+  ++G P    +   EN+R+F+EEQL   + +IGLQ G+NKGASQAGM
Sbjct:    1 MANRGPAYGLSREVQSKIDKKYDPELEERLVQWIIAQCGSGVGQPEPDKTGFQKWLKDGCVLCELINSLYGAN--KPIKTIK-------NSTMA-FKQMEQISLFLKYAEAYGVIKTDMFQTVDLFESKDLAAVQRTLMALGSLAVTKNDGNYRGDPNWFHRKAQENRRDFSEEQLSGGKSVIGLQMGTNKGASQAGM 188          
BLAST of SMED30029432 vs. Ensembl Medaka
Match: tagln3a (transgelin 3 [Source:NCBI gene;Acc:101158980])

HSP 1 Score: 134.42 bits (337), Expect = 2.105e-37
Identity = 81/202 (40.10%), Postives = 114/202 (56.44%), Query Frame = -1
Query:  315 VINMSNRIGDMGMDVQINRKLNSKYDPESEKEVIDWLNKVSG---EHVALGMQSVQQTLKNGQILIKLINKIYEGTDKKPAAALKIRCPLKFNTMSAPFKQMENIQLFLKASEAYGVPRASLFQTVDLYESRNMAQVLNTLLQLGTECQRNEFKGPTCGPKPTYENK-----REFTEEQLRSSQGIIGLQAGSNKGASQAGM 896
            + NM+NR    G+  ++  K+  KYDP+ E+ ++DW+    G   E    G ++ Q+ L +G IL +LIN ++      P     I+   K       FKQME I  FL+A+EAYGV    +FQTVDL+E ++MA V  TL+ LG+     +  G   G +  +  K     REFTEEQLR  Q +IGLQ GSN+GASQ+GM
Sbjct:   11 IPNMANRGPSYGLSREVQEKIEQKYDPDLEQRLVDWIVSQCGGNLEKPQPGKENFQKWLMDGTILCRLINSLF------PRGKEPIK---KIPETQMAFKQMEKISQFLQAAEAYGVTTTDIFQTVDLWEGKDMAAVQRTLMALGSLAVTKD-DGHYKGDRDWFHRKAQGYRREFTEEQLRQGQSLIGLQMGSNRGASQSGM 202          
BLAST of SMED30029432 vs. Planmine SMEST
Match: SMESG000057599.1 (SMESG000057599.1)

HSP 1 Score: 472.626 bits (1215), Expect = 2.170e-168
Identity = 245/324 (75.62%), Postives = 245/324 (75.62%), Query Frame = -1
Query:  153 MSNRIGDMGMDVQINRKLNSKYDPESEKEVIDWLNKVSGEHVALGMQSVQQTLKNGQILIKLINKIYEGTDKKPAAALKIRCPLKFNTMSAPFKQMENIQLFLKASEAYGVPRASLFQTVDLYESRNMAQVLNTLLQLGTECQRNEFKGPTCGPKPTYENKREFTEEQLRSSQGIIGLQAG-------------------------------------------------------------------------------SNKGASQAGMRIGTQRFINDVTTGVEMSDEGKTTIGLQMGPDKSKVDSQSGMSFGAQRHITDSY 887
            MSNRIGDMGMDVQINRKLNSKYDPESEKEVIDWLNKVSGEHVALGMQSVQQTLKNGQILIKLINKIYEGTDKKPAAALKIRCPLKFNTMSAPFKQMENIQLFLKASEAYGVPRASLFQTVDLYESRNMAQVLNTLLQLGTECQRNEFKGPTCGPKPTYENKREFTEEQLRSSQGIIGLQAG                                                                               SNKGASQAGMRIGTQRFINDVTTGVEMSDEGKTTIGLQMGPDKSKVDSQSGMSFGAQRHITDSY
Sbjct:    1 MSNRIGDMGMDVQINRKLNSKYDPESEKEVIDWLNKVSGEHVALGMQSVQQTLKNGQILIKLINKIYEGTDKKPAAALKIRCPLKFNTMSAPFKQMENIQLFLKASEAYGVPRASLFQTVDLYESRNMAQVLNTLLQLGTECQRNEFKGPTCGPKPTYENKREFTEEQLRSSQGIIGLQAGSNKGATQAGMRIGGQRFINDVSSGLEMSDEGKTCIGLQAGSNKGASQTGMSMGAVRHIADIRCDEMSDEAKGVIGLQAGSNKGASQAGMRIGTQRFINDVTTGVEMSDEGKTTIGLQMGPDKSKVDSQSGMSFGAQRHITDSY 324          
BLAST of SMED30029432 vs. Planmine SMEST
Match: SMESG000057599.1 (SMESG000057599.1)

HSP 1 Score: 451.055 bits (1159), Expect = 2.356e-159
Identity = 227/282 (80.50%), Postives = 232/282 (82.27%), Query Frame = -1
Query:  159 MSNRIGDMGMDVQINRKLNSKYDPESEKEVIDWLNKVSGEHVALGMQSVQQTLKNGQILIKLINKIYEGTDKKPAAALKIRCPLKFNTMSAPFKQMENIQLFLKASEAYGVPRASLFQTVDLYESRNMAQVLNTLLQLGTECQRNEFKGPTCGPKPTYENKREFTEEQLRSSQGIIGLQAGSNKGAS---------------------------------------QAGMRIGTQRFINDVTTGVEMSDEGKTTIGLQMGPDKSKVDSQSGMSFGAQRHITD 887
            MSNRIGDMGMDVQINRKLNSKYDPESEKEVIDWLNKVSGEHVALGMQSVQQTLKNGQILIKLINKIYEGTDKKPAAALKIRCPLKFNTMSAPFKQMENIQLFLKASEAYGVPRASLFQTVDLYESRNMAQVLNTLLQLGTECQRNEFKGPTCGPKPTYENKREFTEEQLRSSQGIIGLQAGSNKGAS                                       QAGMRIG QRFINDV++G+EMSDEGKT IGLQ G +K    SQ+GMS GA RHI D
Sbjct:    1 MSNRIGDMGMDVQINRKLNSKYDPESEKEVIDWLNKVSGEHVALGMQSVQQTLKNGQILIKLINKIYEGTDKKPAAALKIRCPLKFNTMSAPFKQMENIQLFLKASEAYGVPRASLFQTVDLYESRNMAQVLNTLLQLGTECQRNEFKGPTCGPKPTYENKREFTEEQLRSSQGIIGLQAGSNKGASQSGMSMGAVRHIADIRCDEMSDEAKGVIGLQAGSNKGATQAGMRIGGQRFINDVSSGLEMSDEGKTCIGLQAGSNKGA--SQTGMSMGAVRHIAD 280          

HSP 2 Score: 149.058 bits (375), Expect = 1.546e-41
Identity = 72/80 (90.00%), Postives = 76/80 (95.00%), Query Frame = -1
Query:  153 EEQLRSSQGIIGLQAGSNKGASQAGMRIGTQRFINDVTTGVEMSDEGKTTIGLQMGPDKSKVDSQSGMSFGAQRHITDSY 392
            +E    ++G+IGLQAGSNKGASQAGMRIGTQRFINDVTTGVEMSDEGKTTIGLQMGPDKSKVDSQSGMSFGAQRHITDSY
Sbjct:  284 DEMSDEAKGVIGLQAGSNKGASQAGMRIGTQRFINDVTTGVEMSDEGKTTIGLQMGPDKSKVDSQSGMSFGAQRHITDSY 363          
BLAST of SMED30029432 vs. Planmine SMEST
Match: SMESG000057600.1 (SMESG000057600.1)

HSP 1 Score: 425.631 bits (1093), Expect = 2.160e-145
Identity = 200/243 (82.30%), Postives = 220/243 (90.53%), Query Frame = -1
Query:  159 MSNRIGDMGMDVQINRKLNSKYDPESEKEVIDWLNKVSGEHVALGMQSVQQTLKNGQILIKLINKIYEGTDKKPAAALKIRCPLKFNTMSAPFKQMENIQLFLKASEAYGVPRASLFQTVDLYESRNMAQVLNTLLQLGTECQRNEFKGPTCGPKPTYENKREFTEEQLRSSQGIIGLQAGSNKGASQAGMRIGTQRFINDVTTGVEMSDEGKTTIGLQMGPDKSKVDSQSGMSFGAQRHITD 887
            MSNRI DMG+DV++N +LNSKYDPESEKEVIDWLN+++GE+++ GMQ+VQQ LKNGQILIKL+N+IY GTDKKP AALKIR PLKFN M APFKQMENIQLFL ASEAYGVPR SLFQTVDLYESRNM QV+NTLLQLGTECQRNEFKGPTCGPKPTYENKREFTEEQLRS QGIIGLQAGSNKGASQ+GMRIGTQRFINDV+TGVE+SDEGK+ IGLQ G +  K  SQ+GMS GA RHI D
Sbjct:    1 MSNRIRDMGIDVKVNGRLNSKYDPESEKEVIDWLNQITGENISFGMQNVQQALKNGQILIKLVNEIYGGTDKKPEAALKIRSPLKFNEMRAPFKQMENIQLFLNASEAYGVPRTSLFQTVDLYESRNMVQVINTLLQLGTECQRNEFKGPTCGPKPTYENKREFTEEQLRSGQGIIGLQAGSNKGASQSGMRIGTQRFINDVSTGVEISDEGKSVIGLQAGSN--KFASQTGMSMGAVRHIAD 241          

HSP 2 Score: 132.109 bits (331), Expect = 9.821e-34
Identity = 61/71 (85.92%), Postives = 68/71 (95.77%), Query Frame = -1
Query:  153 IIGLQAGSNKGASQAGMRIGTQRFINDVTTGVEMSDEGKTTIGLQMGPDKSKVDSQSGMSFGAQRHITDSY 365
            +IGLQAGSNKGA+QAGMRIGTQRFIND+ TGVEMSD+GK  IGLQMGPD+S+VDSQSGMSFGAQRHITD+Y
Sbjct:  572 VIGLQAGSNKGATQAGMRIGTQRFINDIQTGVEMSDKGKNIIGLQMGPDQSQVDSQSGMSFGAQRHITDTY 642          

HSP 3 Score: 102.064 bits (253), Expect = 1.588e-23
Identity = 52/73 (71.23%), Postives = 59/73 (80.82%), Query Frame = -1
Query:  159 SSQGIIGLQAGSNKGASQAGMRIGTQRFINDVTTGVEMSDEGKTTIGLQMGPDKSKVDSQSGMSFGAQRHITD 377
            S + +IGLQAGSNKGA+QAGMRIGTQRFINDV+TGVE+SD GK  IGLQ G +  K  SQ+GMS GA RHI D
Sbjct:  409 SGKNVIGLQAGSNKGATQAGMRIGTQRFINDVSTGVEISDSGKNVIGLQAGSN--KFASQTGMSMGAVRHIAD 479          

HSP 4 Score: 102.064 bits (253), Expect = 1.914e-23
Identity = 51/78 (65.38%), Postives = 61/78 (78.21%), Query Frame = -1
Query:  159 EEQLRSSQGIIGLQAGSNKGASQAGMRIGTQRFINDVTTGVEMSDEGKTTIGLQMGPDKSKVDSQSGMSFGAQRHITD 392
            +E    ++G+IGLQAGSNKGA+QAGMRIGTQRFINDV+TGVE+SD GKT IGLQ G +K    +Q+GM  G QR I D
Sbjct:  483 DEMCDDAKGVIGLQAGSNKGATQAGMRIGTQRFINDVSTGVEISDSGKTVIGLQAGSNKGA--TQAGMRIGTQRFIND 558          

HSP 5 Score: 100.138 bits (248), Expect = 6.673e-23
Identity = 51/73 (69.86%), Postives = 58/73 (79.45%), Query Frame = -1
Query:  159 SSQGIIGLQAGSNKGASQAGMRIGTQRFINDVTTGVEMSDEGKTTIGLQMGPDKSKVDSQSGMSFGAQRHITD 377
            S + +IGLQAGSNKGA+QAGMRIGTQRFINDV+ GVE+SD GK  IGLQ G +  K  SQ+GMS GA RHI D
Sbjct:  290 SGKNVIGLQAGSNKGATQAGMRIGTQRFINDVSAGVEISDSGKNVIGLQAGSN--KFASQTGMSMGAVRHIAD 360          

HSP 6 Score: 99.7525 bits (247), Expect = 8.907e-23
Identity = 50/78 (64.10%), Postives = 60/78 (76.92%), Query Frame = -1
Query:  159 EEQLRSSQGIIGLQAGSNKGASQAGMRIGTQRFINDVTTGVEMSDEGKTTIGLQMGPDKSKVDSQSGMSFGAQRHITD 392
            +E    ++G+IGLQAGSNKGA+QAGMRIGTQRFINDV+TGVE+SD GK  IGLQ G +K    +Q+GM  G QR I D
Sbjct:  364 DEMCDDAKGVIGLQAGSNKGATQAGMRIGTQRFINDVSTGVEISDSGKNVIGLQAGSNKGA--TQAGMRIGTQRFIND 439          

HSP 7 Score: 97.0561 bits (240), Expect = 7.991e-22
Identity = 50/78 (64.10%), Postives = 59/78 (75.64%), Query Frame = -1
Query:  159 EEQLRSSQGIIGLQAGSNKGASQAGMRIGTQRFINDVTTGVEMSDEGKTTIGLQMGPDKSKVDSQSGMSFGAQRHITD 392
            +E   S++G+IGLQAGSNK ASQ GMRIGTQRFINDV+TGVE+SD GK  IGLQ G +K    +Q+GM  G QR I D
Sbjct:  245 DEMCDSAKGVIGLQAGSNKFASQTGMRIGTQRFINDVSTGVEISDSGKNVIGLQAGSNKGA--TQAGMRIGTQRFIND 320          
BLAST of SMED30029432 vs. Planmine SMEST
Match: SMESG000057600.1 (SMESG000057600.1)

HSP 1 Score: 415.231 bits (1066), Expect = 1.905e-141
Identity = 194/236 (82.20%), Postives = 214/236 (90.68%), Query Frame = -1
Query:  159 MGMDVQINRKLNSKYDPESEKEVIDWLNKVSGEHVALGMQSVQQTLKNGQILIKLINKIYEGTDKKPAAALKIRCPLKFNTMSAPFKQMENIQLFLKASEAYGVPRASLFQTVDLYESRNMAQVLNTLLQLGTECQRNEFKGPTCGPKPTYENKREFTEEQLRSSQGIIGLQAGSNKGASQAGMRIGTQRFINDVTTGVEMSDEGKTTIGLQMGPDKSKVDSQSGMSFGAQRHITD 866
            MG+DV++N +LNSKYDPESEKEVIDWLN+++GE+++ GMQ+VQQ LKNGQILIKL+N+IY GTDKKP AALKIR PLKFN M APFKQMENIQLFL ASEAYGVPR SLFQTVDLYESRNM QV+NTLLQLGTECQRNEFKGPTCGPKPTYENKREFTEEQLRS QGIIGLQAGSNKGASQ+GMRIGTQRFINDV+TGVE+SDEGK+ IGLQ G +  K  SQ+GMS GA RHI D
Sbjct:    1 MGIDVKVNGRLNSKYDPESEKEVIDWLNQITGENISFGMQNVQQALKNGQILIKLVNEIYGGTDKKPEAALKIRSPLKFNEMRAPFKQMENIQLFLNASEAYGVPRTSLFQTVDLYESRNMVQVINTLLQLGTECQRNEFKGPTCGPKPTYENKREFTEEQLRSGQGIIGLQAGSNKGASQSGMRIGTQRFINDVSTGVEISDEGKSVIGLQAGSN--KFASQTGMSMGAVRHIAD 234          

HSP 2 Score: 132.109 bits (331), Expect = 7.530e-34
Identity = 61/71 (85.92%), Postives = 68/71 (95.77%), Query Frame = -1
Query:  153 IIGLQAGSNKGASQAGMRIGTQRFINDVTTGVEMSDEGKTTIGLQMGPDKSKVDSQSGMSFGAQRHITDSY 365
            +IGLQAGSNKGA+QAGMRIGTQRFIND+ TGVEMSD+GK  IGLQMGPD+S+VDSQSGMSFGAQRHITD+Y
Sbjct:  565 VIGLQAGSNKGATQAGMRIGTQRFINDIQTGVEMSDKGKNIIGLQMGPDQSQVDSQSGMSFGAQRHITDTY 635          

HSP 3 Score: 102.449 bits (254), Expect = 1.305e-23
Identity = 52/73 (71.23%), Postives = 59/73 (80.82%), Query Frame = -1
Query:  159 SSQGIIGLQAGSNKGASQAGMRIGTQRFINDVTTGVEMSDEGKTTIGLQMGPDKSKVDSQSGMSFGAQRHITD 377
            S + +IGLQAGSNKGA+QAGMRIGTQRFINDV+TGVE+SD GK  IGLQ G +  K  SQ+GMS GA RHI D
Sbjct:  402 SGKNVIGLQAGSNKGATQAGMRIGTQRFINDVSTGVEISDSGKNVIGLQAGSN--KFASQTGMSMGAVRHIAD 472          

HSP 4 Score: 102.064 bits (253), Expect = 1.558e-23
Identity = 51/78 (65.38%), Postives = 61/78 (78.21%), Query Frame = -1
Query:  159 EEQLRSSQGIIGLQAGSNKGASQAGMRIGTQRFINDVTTGVEMSDEGKTTIGLQMGPDKSKVDSQSGMSFGAQRHITD 392
            +E    ++G+IGLQAGSNKGA+QAGMRIGTQRFINDV+TGVE+SD GKT IGLQ G +K    +Q+GM  G QR I D
Sbjct:  476 DEMCDDAKGVIGLQAGSNKGATQAGMRIGTQRFINDVSTGVEISDSGKTVIGLQAGSNKGA--TQAGMRIGTQRFIND 551          

HSP 5 Score: 100.523 bits (249), Expect = 5.437e-23
Identity = 51/73 (69.86%), Postives = 58/73 (79.45%), Query Frame = -1
Query:  159 SSQGIIGLQAGSNKGASQAGMRIGTQRFINDVTTGVEMSDEGKTTIGLQMGPDKSKVDSQSGMSFGAQRHITD 377
            S + +IGLQAGSNKGA+QAGMRIGTQRFINDV+ GVE+SD GK  IGLQ G +  K  SQ+GMS GA RHI D
Sbjct:  283 SGKNVIGLQAGSNKGATQAGMRIGTQRFINDVSAGVEISDSGKNVIGLQAGSN--KFASQTGMSMGAVRHIAD 353          

HSP 6 Score: 100.138 bits (248), Expect = 7.124e-23
Identity = 50/78 (64.10%), Postives = 60/78 (76.92%), Query Frame = -1
Query:  159 EEQLRSSQGIIGLQAGSNKGASQAGMRIGTQRFINDVTTGVEMSDEGKTTIGLQMGPDKSKVDSQSGMSFGAQRHITD 392
            +E    ++G+IGLQAGSNKGA+QAGMRIGTQRFINDV+TGVE+SD GK  IGLQ G +K    +Q+GM  G QR I D
Sbjct:  357 DEMCDDAKGVIGLQAGSNKGATQAGMRIGTQRFINDVSTGVEISDSGKNVIGLQAGSNKGA--TQAGMRIGTQRFIND 432          

HSP 7 Score: 97.4413 bits (241), Expect = 6.642e-22
Identity = 50/78 (64.10%), Postives = 59/78 (75.64%), Query Frame = -1
Query:  159 EEQLRSSQGIIGLQAGSNKGASQAGMRIGTQRFINDVTTGVEMSDEGKTTIGLQMGPDKSKVDSQSGMSFGAQRHITD 392
            +E   S++G+IGLQAGSNK ASQ GMRIGTQRFINDV+TGVE+SD GK  IGLQ G +K    +Q+GM  G QR I D
Sbjct:  238 DEMCDSAKGVIGLQAGSNKFASQTGMRIGTQRFINDVSTGVEISDSGKNVIGLQAGSNKGA--TQAGMRIGTQRFIND 313          
BLAST of SMED30029432 vs. Planmine SMEST
Match: SMESG000067773.1 (SMESG000067773.1)

HSP 1 Score: 311.227 bits (796), Expect = 2.895e-104
Identity = 149/243 (61.32%), Postives = 188/243 (77.37%), Query Frame = -1
Query:  159 MSNRIGDMGMDVQINRKLNSKYDPESEKEVIDWLNKVSGEHVALGMQSVQQTLKNGQILIKLINKIYEGTDKKPAAALKIRCPLKFNTMSAPFKQMENIQLFLKASEAYGVPRASLFQTVDLYESRNMAQVLNTLLQLGTECQRNEFKGPTCGPKPTYENKREFTEEQLRSSQGIIGLQAGSNKGASQAGMRIGTQRFINDVTTGVEMSDEGKTTIGLQMGPDKSKVDSQSGMSFGAQRHITD 887
            MSNR  D G+DV I+R+LNSKYDPESE+E I+W+N+++G  + LG ++VQ  LKNGQIL+KLIN IYE T   P +A + + P ++NTM+APFKQMENIQ+FL+A+E+YGVPR SLFQTVDL+ESRNMAQVLN++LQLGTECQR+ F GP CG KPT  N R+F++EQLR  +GII LQ+G+NK ASQ+GM  G  R I D+    +++D GK  IGLQMG +  K  SQ GMS GA RHI D
Sbjct:    1 MSNRTQDFGIDVAISRRLNSKYDPESEQEAIEWINQLTGSSIPLGRENVQHHLKNGQILVKLINVIYERTSNLPDSARREKYPFRYNTMTAPFKQMENIQIFLRAAESYGVPRQSLFQTVDLFESRNMAQVLNSILQLGTECQRSGFSGPVCGAKPTSTNYRQFSDEQLRMGEGIINLQSGTNKFASQSGMSFGGVRHIADIRCD-DLNDSGKGIIGLQMGTN--KYASQKGMSIGAVRHIAD 240          

HSP 2 Score: 88.5817 bits (218), Expect = 1.793e-19
Identity = 42/79 (53.16%), Postives = 55/79 (69.62%), Query Frame = -1
Query:  153 EQLRSSQGIIGLQAGSNKGASQAGMRIGTQRFINDVTTGVEMSDEGKTTIGLQMGPDKSKVDSQSGMSFGAQRHITDSY 389
            E  + +Q +I LQ G+N GA+Q GMRIGTQR I D++ G+E +      + LQMGP   +V +QSGMSFGAQRHI DS+
Sbjct:  284 ESNKEAQSVISLQYGTNLGANQTGMRIGTQRHITDISCGIEPNKMSSNMVSLQMGPSMKEVANQSGMSFGAQRHINDSH 362          
The following BLAST results are available for this feature:
BLAST of SMED30029432 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
TAGLN1.636e-3741.92transgelin [Source:HGNC Symbol;Acc:HGNC:11553][more]
TAGLN1.636e-3741.92transgelin [Source:HGNC Symbol;Acc:HGNC:11553][more]
TAGLN1.636e-3741.92transgelin [Source:HGNC Symbol;Acc:HGNC:11553][more]
TAGLN1.636e-3741.92transgelin [Source:HGNC Symbol;Acc:HGNC:11553][more]
TAGLN1.636e-3741.92transgelin [Source:HGNC Symbol;Acc:HGNC:11553][more]
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BLAST of SMED30029432 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
cpn-18.751e-3134.50CPN-1; CalPoNin [Source:UniProtKB/TrEMBL;Acc:G5EF... [more]
cpn-18.751e-3134.50CPN-1; CalPoNin [Source:UniProtKB/TrEMBL;Acc:G5EF... [more]
cpn-18.751e-3134.50CPN-1; CalPoNin [Source:UniProtKB/TrEMBL;Acc:G5EF... [more]
cpn-21.158e-2131.03Transgelin [Source:UniProtKB/TrEMBL;Acc:A0A0K3AWU... [more]
cpn-21.158e-2131.03Transgelin [Source:UniProtKB/TrEMBL;Acc:A0A0K3AWU... [more]
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BLAST of SMED30029432 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Mp202.678e-4645.60gene:FBgn0002789 transcript:FBtr0087745[more]
Mp203.215e-4645.60gene:FBgn0002789 transcript:FBtr0345532[more]
Chd649.121e-3537.00gene:FBgn0035499 transcript:FBtr0073270[more]
CG50235.542e-3444.91gene:FBgn0038774 transcript:FBtr0083866[more]
Chd641.854e-3137.30gene:FBgn0035499 transcript:FBtr0310531[more]
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BLAST of SMED30029432 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
tagln22.227e-4341.18transgelin 2 [Source:ZFIN;Acc:ZDB-GENE-020802-2][more]
tagln25.072e-4341.79transgelin 2 [Source:ZFIN;Acc:ZDB-GENE-020802-2][more]
tagln25.072e-4341.79transgelin 2 [Source:ZFIN;Acc:ZDB-GENE-020802-2][more]
tagln3a4.448e-3739.90transgelin 3a [Source:ZFIN;Acc:ZDB-GENE-060531-53][more]
tagln3b8.415e-3741.21transgelin 3b [Source:ZFIN;Acc:ZDB-GENE-030131-419... [more]
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BLAST of SMED30029432 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
tagln22.383e-3840.20transgelin 2 [Source:NCBI gene;Acc:394981][more]
kcnb26.655e-3739.90potassium channel, voltage gated Shab related subf... [more]
kcnb27.224e-3739.90potassium channel, voltage gated Shab related subf... [more]
tfdp15.638e-3135.71transcription factor Dp-1 [Source:Xenbase;Acc:XB-G... [more]
cnn11.998e-2834.31calponin 1, basic, smooth muscle [Source:NCBI gene... [more]
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BLAST of SMED30029432 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Tagln39.858e-3840.59transgelin 3 [Source:MGI Symbol;Acc:MGI:1926784][more]
Tagln1.988e-3741.41transgelin [Source:MGI Symbol;Acc:MGI:106012][more]
Tagln21.801e-3539.39transgelin 2 [Source:MGI Symbol;Acc:MGI:1312985][more]
Tagln21.801e-3539.39transgelin 2 [Source:MGI Symbol;Acc:MGI:1312985][more]
Cnn34.147e-3136.48calponin 3, acidic [Source:MGI Symbol;Acc:MGI:1919... [more]
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BLAST of SMED30029432 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q24799|MYPH_ECHGR1.711e-4845.54Myophilin OS=Echinococcus granulosus OX=6210 PE=2 ... [more]
sp|P14318|MP20_DROME2.684e-4545.60Muscle-specific protein 20 OS=Drosophila melanogas... [more]
sp|P19966|TAGL_CHICK5.502e-3741.26Transgelin OS=Gallus gallus OX=9031 GN=TAGLN PE=1 ... [more]
sp|Q9R1Q8|TAGL3_MOUSE6.896e-3740.59Transgelin-3 OS=Mus musculus OX=10090 GN=Tagln3 PE... [more]
sp|P37805|TAGL3_RAT7.118e-3740.59Transgelin-3 OS=Rattus norvegicus OX=10116 GN=Tagl... [more]
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BLAST of SMED30029432 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0X3NLR91.379e-11970.37Transgelin OS=Schistocephalus solidus OX=70667 GN=... [more]
K4IR161.707e-11467.49Calponin (Fragment) OS=Solen grandis OX=165599 PE=... [more]
A0A068YJ883.401e-11468.72Transgelin OS=Echinococcus multilocularis OX=6211 ... [more]
A0A5J4NR988.819e-11469.14Calponin OS=Paragonimus westermani OX=34504 GN=DEA... [more]
W6V0C94.089e-11367.90Transgelin OS=Echinococcus granulosus OX=6210 GN=E... [more]
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BLAST of SMED30029432 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
tagln2.529e-3941.29transgelin-like [Source:NCBI gene;Acc:103045968][more]
tagln3a5.594e-3840.20transgelin-3-like [Source:NCBI gene;Acc:103024278][more]
TAGLN32.806e-3540.70transgelin 3 [Source:NCBI gene;Acc:103030448][more]
cnn1b7.682e-3034.73calponin 1 [Source:NCBI gene;Acc:103040806][more]
cnn3a1.136e-2935.71calponin 3 [Source:NCBI gene;Acc:103024651][more]
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BLAST of SMED30029432 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30029432 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1
Match NameE-valueIdentityDescription
SCP11.979e-1730.46Component of yeast cortical actin cytoskeleton; bi... [more]
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BLAST of SMED30029432 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO306563.692e-4243.89Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO467122.731e-4040.39Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO440754.337e-3940.58Myophilin-like protein; Predicted protein [Source... [more]
EDO466585.778e-3440.00Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO306546.118e-2951.43Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of SMED30029432 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
tagln1.928e-3841.35transgelin [Source:NCBI gene;Acc:101169057][more]
tagln2.085e-3841.35transgelin [Source:NCBI gene;Acc:101169057][more]
ENSORLT00000011293.27.193e-3841.41transgelin [Source:NCBI gene;Acc:101159525][more]
ENSORLT00000028673.18.369e-3841.41transgelin [Source:NCBI gene;Acc:101159525][more]
tagln3a2.105e-3740.10transgelin 3 [Source:NCBI gene;Acc:101158980][more]
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BLAST of SMED30029432 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000057599.12.170e-16875.62SMESG000057599.1[more]
SMESG000057599.12.356e-15980.50SMESG000057599.1[more]
SMESG000057600.12.160e-14582.30SMESG000057600.1[more]
SMESG000057600.11.905e-14182.20SMESG000057600.1[more]
SMESG000067773.12.895e-10461.32SMESG000067773.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30029432 ID=SMED30029432|Name=SMED30029432|organism=Schmidtea mediterranea sexual|type=transcript|length=1064bp
CGGCCGCTTTGAAACTTGTAAAAGTCGTGAACTCAAAGTTATAAATAAAA
TAAAGTAACAGGGCGGATATATTTCTTTATTAGCTATTATTTCTTGAGAA
ATTCAGCCAATAAGTCAAAAGCATGCGATTTAGCATTTTTCTTTTAGAAT
TAGTATGAATCTGTGATGTGTCTTTGGGCACCAAAACTCATTCCCGATTG
TGAATCGACTTTGCTCTTGTCAGGACCCATTTGCAAACCAATTGTGGTTT
TGCCTTCATCGCTCATTTCAACACCAGTTGTGACATCATTGATGAATCTT
TGAGTACCAATTCTCATTCCGGCTTGTGAAGCGCCTTTATTTGAACCAGC
TTGTAAACCAATTATTCCTTGACTGGATCTTAGTTGTTCTTCAGTAAATT
CTCTCTTGTTTTCATATGTCGGTTTTGGGCCACATGTTGGACCTTTGAAT
TCATTTCTTTGACACTCTGTACCTAGCTGTAATAACGTATTCAATACTTG
AGCCATATTTCTACTTTCATATAAATCTACAGTTTGGAATAAACTTGCTC
TCGGAACACCATATGCTTCCGACGCTTTCAAGAACAATTGAATATTTTCC
ATTTGTTTAAATGGAGCACTCATTGTATTGAATTTCAATGGACAACGGAT
TTTTAAAGCTGCAGCAGGTTTCTTGTCAGTACCCTCGTAAATTTTGTTGA
TCAATTTGATAAGAATTTGTCCGTTTTTCAGTGTTTGCTGGACGCTTTGC
ATACCCAAGGCAACATGTTCCCCAGAAACTTTATTTAACCAATCAATGAC
TTCCTTTTCAGACTCAGGATCGTATTTACTGTTCAACTTTCGGTTTATTT
GAACGTCCATGCCCATATCTCCTATTCTATTCGACATGTTTATTACCTAC
TTTGATTATAATAATTATAATAAGGGAAACTAAAACTAATTTAATCTATT
TTTGGCTACGTTGGGATTTAAATATAAAATTTTCTTGCATACTTTCTTTA
TTAAAGCGAAGGAACCGGTCGATAACCGGGTTCAGTGTCCTGTGTGTGCA
AAGGTATTTATAAA
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protein sequence of SMED30029432-orf-1

>SMED30029432-orf-1 ID=SMED30029432-orf-1|Name=SMED30029432-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=246bp
MSNRIGDMGMDVQINRKLNSKYDPESEKEVIDWLNKVSGEHVALGMQSVQ
QTLKNGQILIKLINKIYEGTDKKPAAALKIRCPLKFNTMSAPFKQMENIQ
LFLKASEAYGVPRASLFQTVDLYESRNMAQVLNTLLQLGTECQRNEFKGP
TCGPKPTYENKREFTEEQLRSSQGIIGLQAGSNKGASQAGMRIGTQRFIN
DVTTGVEMSDEGKTTIGLQMGPDKSKVDSQSGMSFGAQRHITDSY*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000101muscle cell
PLANA:0000420parapharyngeal region
PLANA:0000428musculature system
PLANA:0000478pharynx musculature
Vocabulary: INTERPRO
TermDefinition
IPR036872CH_dom_sf
IPR003096SM22_calponin
IPR001715CH-domain
IPR000557Calponin_repeat
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003096Smooth muscle protein/calponinPRINTSPR00888SM22CALPONINcoord: 164..177
score: 43.7
coord: 94..110
score: 57.44
coord: 3..17
score: 23.92
coord: 110..125
score: 46.32
coord: 131..145
score: 31.37
coord: 177..192
score: 74.63
IPR001715Calponin homology domainSMARTSM00033ch_5coord: 26..138
e-value: 6.1E-22
score: 88.9
IPR001715Calponin homology domainPFAMPF00307CHcoord: 25..143
e-value: 2.5E-21
score: 75.9
IPR001715Calponin homology domainPROSITEPS50021CHcoord: 24..143
score: 17.847
IPR001715Calponin homology domainCDDcd00014CHcoord: 25..143
e-value: 2.5973E-26
score: 96.6111
IPR000557Calponin repeatPFAMPF00402Calponincoord: 176..199
e-value: 4.9E-11
score: 42.1
coord: 216..241
e-value: 1.9E-11
score: 43.4
IPR000557Calponin repeatPROSITEPS01052CALPONIN_1coord: 176..194
IPR000557Calponin repeatPROSITEPS51122CALPONIN_2coord: 176..200
score: 10.498
IPR000557Calponin repeatPROSITEPS51122CALPONIN_2coord: 216..242
score: 7.239
IPR036872CH domain superfamilyGENE3DG3DSA:1.10.418.10coord: 8..176
e-value: 3.8E-37
score: 129.1
IPR036872CH domain superfamilySUPERFAMILYSSF47576Calponin-homology domain, CH-domaincoord: 16..186
NoneNo IPR availablePANTHERPTHR47385FAMILY NOT NAMEDcoord: 4..201
NoneNo IPR availablePANTHERPTHR47385:SF3coord: 4..201