Baculoviral IAP repeat-containing protein 7
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30029426 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 21
Homology
BLAST of Baculoviral IAP repeat-containing protein 7 vs. Ensembl Zebrafish
Match: birc2 (baculoviral IAP repeat containing 2 [Source:ZFIN;Acc:ZDB-GENE-030825-6]) HSP 1 Score: 50.447 bits (119), Expect = 2.424e-6 Identity = 22/75 (29.33%), Postives = 46/75 (61.33%), Query Frame = 1 Query: 559 SRLFKEWIRKSD------FIEDKLTEKLNDVKKRYLTCSICKENQVSVLYLPCGHVIVCSKCQDKNNRKCQACSA 765 + +F+ WI+K+D ++ + E+L +++ TC +C + +V+++++PCGH++VC +C + RKC C Sbjct: 550 AEVFRNWIKKNDVYLLRELMDLPMEEQLRRLQEER-TCKVCMDKEVNIVFIPCGHLVVCKECA-PSLRKCPICRG 622
BLAST of Baculoviral IAP repeat-containing protein 7 vs. Ensembl Xenopus
Match: arsa.1 (arylsulfatase A, gene 1 [Source:Xenbase;Acc:XB-GENE-5959463]) HSP 1 Score: 48.521 bits (114), Expect = 9.754e-6 Identity = 21/52 (40.38%), Postives = 35/52 (67.31%), Query Frame = 1 Query: 616 EKLNDVKKRYLTCSICKENQVSVLYLPCGHVIVCSKCQDKNNRKCQACSAPI 771 E+L +K+ + C +C +N VS++++PCGH++VC++C N R C C A I Sbjct: 309 EQLRQLKEERM-CKVCMDNDVSMVFVPCGHLVVCTECA-PNLRHCPICRAAI 358
BLAST of Baculoviral IAP repeat-containing protein 7 vs. Ensembl Mouse
Match: Birc2 (baculoviral IAP repeat-containing 2 [Source:MGI Symbol;Acc:MGI:1197009]) HSP 1 Score: 48.9062 bits (115), Expect = 9.138e-6 Identity = 26/76 (34.21%), Postives = 42/76 (55.26%), Query Frame = 1 Query: 556 YSRLFKE----WIRKSDFIEDKLTEKLNDVKKRYLTCSICKENQVSVLYLPCGHVIVCSKCQDKNNRKCQACSAPI 771 Y LF E +I D L E+L +++ TC +C + +VS++++PCGH++VC +C + RKC C I Sbjct: 530 YENLFVEKNMKYIPTEDVSGLSLEEQLRRLQEER-TCKVCMDREVSIVFIPCGHLVVCQECA-PSLRKCPICRGTI 603
BLAST of Baculoviral IAP repeat-containing protein 7 vs. Ensembl Mouse
Match: Birc2 (baculoviral IAP repeat-containing 2 [Source:MGI Symbol;Acc:MGI:1197009]) HSP 1 Score: 48.9062 bits (115), Expect = 9.138e-6 Identity = 26/76 (34.21%), Postives = 42/76 (55.26%), Query Frame = 1 Query: 556 YSRLFKE----WIRKSDFIEDKLTEKLNDVKKRYLTCSICKENQVSVLYLPCGHVIVCSKCQDKNNRKCQACSAPI 771 Y LF E +I D L E+L +++ TC +C + +VS++++PCGH++VC +C + RKC C I Sbjct: 530 YENLFVEKNMKYIPTEDVSGLSLEEQLRRLQEER-TCKVCMDREVSIVFIPCGHLVVCQECA-PSLRKCPICRGTI 603
BLAST of Baculoviral IAP repeat-containing protein 7 vs. UniProt/SwissProt
Match: sp|P40629|413R_IIV6 (Putative RING finger protein 413R OS=Invertebrate iridescent virus 6 OX=176652 GN=EF2 PE=4 SV=2) HSP 1 Score: 50.8322 bits (120), Expect = 4.459e-6 Identity = 31/90 (34.44%), Postives = 43/90 (47.78%), Query Frame = 1 Query: 538 LRLNPEYSRLFKEWIRKSDFIEDKLTEKLNDVKKRYLTCSICKENQVSVLYLPCGHVIVCSKCQ------------DKNNRKCQACSAPI 771 + + PE L + K + +EDKLT + LTC IC NQV +L +PCGH+I+C+ C + N KC C PI Sbjct: 115 IDITPEIEILNQNEKEKFNQVEDKLT-----TLSKNLTCIICLTNQVQILTIPCGHLIMCNPCSLNLNNSVCTRGVNSNYEKCPKCRTPI 199
BLAST of Baculoviral IAP repeat-containing protein 7 vs. TrEMBL
Match: I1ZIH9 (IAP domain containing-1 (Fragment) OS=Schmidtea mediterranea OX=79327 PE=2 SV=1) HSP 1 Score: 62.7734 bits (151), Expect = 3.773e-9 Identity = 26/50 (52.00%), Postives = 37/50 (74.00%), Query Frame = 1 Query: 646 LTCSICKENQVSVLYLPCGHVIVCSKCQDKNNRKCQACSAPINEYHAIYV 795 LTC+ CK+NQVS+LY PC HV++C C + + CQ C+ PI++YH IY+ Sbjct: 7 LTCNFCKKNQVSILYEPCYHVVICRTCFVSSMKICQVCNVPIHQYHLIYI 56
BLAST of Baculoviral IAP repeat-containing protein 7 vs. Ensembl Cavefish
Match: birc2 (baculoviral IAP repeat containing 2 [Source:NCBI gene;Acc:103025481]) HSP 1 Score: 50.0618 bits (118), Expect = 2.670e-6 Identity = 22/75 (29.33%), Postives = 46/75 (61.33%), Query Frame = 1 Query: 559 SRLFKEWIRKSD------FIEDKLTEKLNDVKKRYLTCSICKENQVSVLYLPCGHVIVCSKCQDKNNRKCQACSA 765 + +F+ WI+K+D ++ + E+L +++ TC +C + +V+++++PCGH++VC +C + RKC C Sbjct: 534 AEVFRNWIQKNDVYLLRELMDLPMEEQLRRLQEER-TCKVCMDKEVNIVFIPCGHLVVCKECA-PSLRKCPICRG 606
BLAST of Baculoviral IAP repeat-containing protein 7 vs. Ensembl Sea Lamprey
Match: birc2 (baculoviral IAP repeat containing 2 [Source:ZFIN;Acc:ZDB-GENE-030825-6]) HSP 1 Score: 46.9802 bits (110), Expect = 5.161e-6 Identity = 20/50 (40.00%), Postives = 35/50 (70.00%), Query Frame = 1 Query: 616 EKLNDVKKRYLTCSICKENQVSVLYLPCGHVIVCSKCQDKNNRKCQACSA 765 E+L +++++ TC +C + +VSV+++PCGH++VC C + RKC C A Sbjct: 518 EELKRLQEQH-TCKVCMDKEVSVVFIPCGHLVVCRDCAS-SLRKCPICRA 565
BLAST of Baculoviral IAP repeat-containing protein 7 vs. Planmine SMEST
Match: SMESG000067789.1 (SMESG000067789.1) HSP 1 Score: 425.246 bits (1092), Expect = 3.400e-151 Identity = 209/211 (99.05%), Postives = 209/211 (99.05%), Query Frame = 1 Query: 163 MNFSGRIVQYDCGTADRQLKNLSKKSENENSVKTKNKTIISEQKPSMEFIELKSTVEDFINTYDTFTDKEIINILKIFKNPSIEYLIDIFNYDKCILKLSSYLMYLDKTGMDNDSLNEYFWDCMELRLNPEYSRLFKEWIRKSDFIEDKLTEKLNDVKKRYLTCSICKENQVSVLYLPCGHVIVCSKCQDKNNRKCQACSAPINEYHAIYV 795 MN SGRIVQYDCGTADRQLKNLSKKSENENSVKTKNKTIISEQKPSMEFIE KSTVEDFINTYDTFTDKEIINILKIFKNPSIEYLIDIFNYDKCILKLSSYLMYLDKTGMDNDSLNEYFWDCMELRLNPEYSRLFKEWIRKSDFIEDKLTEKLNDVKKRYLTCSICKENQVSVLYLPCGHVIVCSKCQDKNNRKCQACSAPINEYHAIYV Sbjct: 1 MNCSGRIVQYDCGTADRQLKNLSKKSENENSVKTKNKTIISEQKPSMEFIEFKSTVEDFINTYDTFTDKEIINILKIFKNPSIEYLIDIFNYDKCILKLSSYLMYLDKTGMDNDSLNEYFWDCMELRLNPEYSRLFKEWIRKSDFIEDKLTEKLNDVKKRYLTCSICKENQVSVLYLPCGHVIVCSKCQDKNNRKCQACSAPINEYHAIYV 211
BLAST of Baculoviral IAP repeat-containing protein 7 vs. Planmine SMEST
Match: SMESG000067789.1 (SMESG000067789.1) HSP 1 Score: 316.62 bits (810), Expect = 5.319e-109 Identity = 158/161 (98.14%), Postives = 159/161 (98.76%), Query Frame = 1 Query: 163 MNFSGRIVQYDCGTADRQLKNLSKKSENENSVKTKNKTIISEQKPSMEFIELKSTVEDFINTYDTFTDKEIINILKIFKNPSIEYLIDIFNYDKCILKLSSYLMYLDKTGMDNDSLNEYFWDCMELRLNPEYSRLFKEWIRKSDFIEDKLTEKLNDVKKRY 645 MN SGRIVQYDCGTADRQLKNLSKKSENENSVKTKNKTIISEQKPSMEFIE KSTVEDFINTYDTFTDKEIINILKIFKNPSIEYLIDIFNYDKCILKLSSYLMYLDKTGMDNDSLNEYFWDCMELRLNPEYSRLFKEWIRKSDFIEDKLTEKLNDVKKR+ Sbjct: 1 MNCSGRIVQYDCGTADRQLKNLSKKSENENSVKTKNKTIISEQKPSMEFIEFKSTVEDFINTYDTFTDKEIINILKIFKNPSIEYLIDIFNYDKCILKLSSYLMYLDKTGMDNDSLNEYFWDCMELRLNPEYSRLFKEWIRKSDFIEDKLTEKLNDVKKRF 161
BLAST of Baculoviral IAP repeat-containing protein 7 vs. Planmine SMEST
Match: SMESG000031949.1 (SMESG000031949.1) HSP 1 Score: 129.413 bits (324), Expect = 1.143e-35 Identity = 69/182 (37.91%), Postives = 104/182 (57.14%), Query Frame = 1 Query: 262 TKNKTIISEQKP-SMEFIELKSTVEDFINTYDTFTDKEII---NILKIFKNPSIEYLIDIFNYDKCILKLSSYLMYLDKTGMDNDSLNEYFWDCMELRLNPEYSRLFKEWIRKSDFIEDKLTEKLNDVKKRYLTCSICKENQVSVLYLPCGHVIVCSKCQDKNNRKCQACSAPINEYHAIYV 795 TKN + P ++ +K ++ N D+F + + I +LK F P I+Y D F YDK +LKL S+++ + ++ D++N FW CMELRL+ EY +WI + ++ K EK ++ YL C +CK QVS+LYLPCGH+I+C+KC +N + CQ C I E H IY+ Sbjct: 12 TKNIYVSDSDGPRAIHLNNVKERIKTNANISDSFENLDFIFLGKLLKSFNEPLIQYFHDTFGYDKPLLKLVSFILNSYEIKVNKDNMNFLFWTCMELRLDFEYRSEINKWISNFEQLKLKAQEKFQNIASFYLKCFLCKIQQVSILYLPCGHLILCTKCFQRNTKYCQICQILIEEAHTIYL 193
BLAST of Baculoviral IAP repeat-containing protein 7 vs. Planmine SMEST
Match: SMESG000067788.1 (SMESG000067788.1) HSP 1 Score: 123.25 bits (308), Expect = 3.532e-33 Identity = 62/156 (39.74%), Postives = 97/156 (62.18%), Query Frame = 1 Query: 328 VEDFINTYDTFTDKEIINILKIFKNPSIEYLIDIFNYDKCILKLSSYLMYLDKTGMDNDSLNEYFWDCMELRLNPEYSRLFKEWIRKSDFIEDKLTEKLNDVKKRYLTCSICKENQVSVLYLPCGHVIVCSKCQDKNNRKCQACSAPINEYHAIYV 795 +++ Y K ++ ILK+FKN SI++ + F Y+K ILKL S+LM +K +++++LN+YFW M L S+L D IE ++ E + + K+ LTC C++N VS+LYLPCGHV++C+KC + + CQAC + I E+H IY+ Sbjct: 69 IDELFRNYAKLNSKILLEILKLFKNSSIKFCHETFGYNKKILKLCSFLMERNKVNINHENLNDYFWSFMNL------SQL--------DGIEKEMCEIVKERNKQLLTCMGCQKNSVSILYLPCGHVVMCNKCYQISTKHCQACGSSIEEFHVIYI 210
BLAST of Baculoviral IAP repeat-containing protein 7 vs. Planmine SMEST
Match: SMESG000032006.1 (SMESG000032006.1) HSP 1 Score: 120.939 bits (302), Expect = 1.431e-32 Identity = 58/159 (36.48%), Postives = 95/159 (59.75%), Query Frame = 1 Query: 316 LKSTVEDFINTYDTFTDKEIINILKIFKNPSIEYLIDIFNYDKCILKLSSYLMYLDKTGMDNDSLNEYFWDCMELRLNPEYSRLFKEWIRKSDFIEDKLTEKLNDVKKRYLTCSICKENQVSVLYLPCGHVIVCSKCQDKNNRKCQACSAPINEYHAIY 792 +++ + DF+ Y+ K + I+K F N +E L + F Y++ +LKL+S ++ +++ + +NE FWDCM LRL+ ++ K W+ D I L + N + K YLTC C+ N VS+LYLPCG +++CS+C + + KCQ C ++E H IY Sbjct: 25 IENEIFDFLENYEILPLKTVQRIVKSFANEYVEILHEKFGYEQSVLKLASCVISINQIDESLERMNEIFWDCMNLRLDKSCGKIIKSWMLNIDRIVLDLKKISNKLSKEYLTCCACQLNPVSILYLPCGDIVICSECYNDSVVKCQGCQRHVDEMHKIY 183 The following BLAST results are available for this feature:
BLAST of Baculoviral IAP repeat-containing protein 7 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of Baculoviral IAP repeat-containing protein 7 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of Baculoviral IAP repeat-containing protein 7 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of Baculoviral IAP repeat-containing protein 7 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 1
BLAST of Baculoviral IAP repeat-containing protein 7 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 1
BLAST of Baculoviral IAP repeat-containing protein 7 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 2
BLAST of Baculoviral IAP repeat-containing protein 7 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 1
BLAST of Baculoviral IAP repeat-containing protein 7 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 1
BLAST of Baculoviral IAP repeat-containing protein 7 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 1
BLAST of Baculoviral IAP repeat-containing protein 7 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 1
BLAST of Baculoviral IAP repeat-containing protein 7 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of Baculoviral IAP repeat-containing protein 7 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of Baculoviral IAP repeat-containing protein 7 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of Baculoviral IAP repeat-containing protein 7 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30029426 ID=SMED30029426|Name=Baculoviral IAP repeat-containing protein 7|organism=Schmidtea mediterranea sexual|type=transcript|length=1116bpback to top protein sequence of SMED30029426-orf-1 >SMED30029426-orf-1 ID=SMED30029426-orf-1|Name=SMED30029426-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=212bp MNFSGRIVQYDCGTADRQLKNLSKKSENENSVKTKNKTIISEQKPSMEFIback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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