Propionyl-CoA synthetase

Overview
NamePropionyl-CoA synthetase
Smed IDSMED30029401
Length (bp)1810
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Propionyl-CoA synthetase (SMED30029401) t-SNE clustered cells

Violin plots show distribution of expression levels for Propionyl-CoA synthetase (SMED30029401) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Propionyl-CoA synthetase (SMED30029401) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Propionyl-CoA synthetase (SMED30029401) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30029401

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 12

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
parapharyngeal regionSMED30029401h1SMcG0020266 SmedASXL_048827SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
nervous systemSMED30029401h1SMcG0020266 dd_Smed_v4_12050_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30029401h1SMcG0020266 dd_Smed_v4_12050_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30029401h1SMcG0020266 dd_Smed_v4_12050_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30029401h1SMcG0020269 dd_Smed_v4_12050_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30029401h1SMcG0020269 dd_Smed_v4_12050_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30029401h1SMcG0020269 dd_Smed_v4_12050_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parapharyngeal regionSMED30029401h1SMcG0020266 dd_Smed_v6_793_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
reproductive organSMED30029401h1SMcG0020266 Contig42230newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30029401h1SMcG0020266 Contig42230uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30029401h1SMcG0020267 Contig42230newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30029401h1SMcG0020267 Contig42230uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Propionyl-CoA synthetase vs. Ensembl Human
Match: ACSS3 (acyl-CoA synthetase short chain family member 3 [Source:HGNC Symbol;Acc:HGNC:24723])

HSP 1 Score: 302.753 bits (774), Expect = 1.604e-98
Identity = 172/473 (36.36%), Postives = 263/473 (55.60%), Query Frame = 3 
Query:  249 KFIITTNFGYEPERTVNYKEIVDEAINKSQFCPDYCIVYNRPNTKCATMIDSRDLDWETEIGKATPYPPVALESGDPIFIIYTSGTLGEPKGLVMTVGGFVVSNKYFMKYVLGLNRRDCWWMFGDYGWISGQTTMTFGPLLIGCSTILYEGKELSSHHFTGIGRIIDQNNVTGFSAAPTYFRFLKNKDSEKDIKPSKAQLRF--VCIGGELSNTKLKEWVSNVFLCPVYDLWGQTELG--IIATGPGLGFKNISSNGLS----PMLSVHPKVVNSATGEQLPNETIGRMLLKIPLCPSAASSMYNDHDRFLKTYFSKXXXXXXXXXXXXIMNDGTLQILARADDVISVSAGRICGGALEEVCLMNEDLANAAFVALEDPINGQVPFAFLEKKKDRNINNEALIIESVKLVKKEIGSFTLFHQGVVVHDLPKTKTGKILRKTLYNMLNGQDWKTPATVENPKVFEEIYSILIEA 1643
            K ++T +FG EP R V Y  +V+EA+   Q  PD  ++YNRPN +   +   RDLDW+ E+ KA  +  V + S  P++I+YTSGT G PKG++   GG+ V   + M  + GL   + WW   D GW+ G + + +GPLL G +T+LYEGK + +       R++ ++ V     APT  R ++ +D    +    +  RF  + + GE  + +  EW  NVF  PV D W QTE G  I A+  GLG       G +    P  +V   ++     ++L    +G +++K+PL P A S ++ + + F   YF K+ GYYDTMD G +  +G L +++R DDVI+V+  RI  GA+EE  L +  +A+ A V  EDP+ G VP A    +KD N   E ++ E VK V++ IG    F   V V  LPKT++GKI R  L  ++NG+ +K  +T+E+P +F  +  +L +A
Sbjct:  216 KVVVTASFGIEPGRRVEYVPLVEEALKIGQHKPDKILIYNRPNMEAVPLAPGRDLDWDEEMAKAQSHDCVPVLSEHPLYILYTSGTTGLPKGVIRPTGGYAVMLHWSMSSIYGLQPGEVWWAASDLGWVVGHSYICYGPLLHGNTTVLYEGKPVGTPDAGAYFRVLAEHGVAALFTAPTAIRAIRQQDPGAALGKQYSLTRFKTLFVAGERCDVETLEWSKNVFRVPVLDHWWQTETGSPITASCVGLGNSKTPPPGQAGKSVPGYNV---MILDDNMQKLKARCLGNIVVKLPLPPGAFSGLWKNQEAFKHLYFEKFPGYYDTMDAGYMDEEGYLYVMSRVDDVINVAGHRISAGAIEESILSHGTVADCAVVGKEDPLKGHVPLALCVLRKDINATEEQVLEEIVKHVRQNIGPVAAFRNAVFVKQLPKTRSGKIPRSALSAIVNGKPYKITSTIEDPSIFGHVEEMLKQA 685

HSP 2 Score: 78.5666 bits (192), Expect = 1.604e-98
Identity = 37/84 (44.05%), Postives = 57/84 (67.86%), Query Frame = 2 
Query:    2 PVINATEKVSYKQLLTLVENLAGVMVNKCHVQKGDRVLLYVPVTVQGIAAILACTRIGAVYVVTFGGFAPDLIAARIKHTEPKI 253
            PV N     +YK++L  V  LAGV+V K  ++KGD V++Y+P+  Q +  +LAC RIGA++ + FGGFA   +++RI H +PK+
Sbjct:  135 PVTNTKATFTYKEVLEQVSKLAGVLV-KHGIKKGDTVVIYMPMIPQAMYTMLACARIGAIHSLIFGGFASKELSSRIDHVKPKV 217
[Close]
BLAST of Propionyl-CoA synthetase vs. Ensembl Human
Match: ACSS3 (acyl-CoA synthetase short chain family member 3 [Source:HGNC Symbol;Acc:HGNC:24723])

HSP 1 Score: 302.753 bits (774), Expect = 1.714e-98
Identity = 172/473 (36.36%), Postives = 263/473 (55.60%), Query Frame = 3 
Query:  249 KFIITTNFGYEPERTVNYKEIVDEAINKSQFCPDYCIVYNRPNTKCATMIDSRDLDWETEIGKATPYPPVALESGDPIFIIYTSGTLGEPKGLVMTVGGFVVSNKYFMKYVLGLNRRDCWWMFGDYGWISGQTTMTFGPLLIGCSTILYEGKELSSHHFTGIGRIIDQNNVTGFSAAPTYFRFLKNKDSEKDIKPSKAQLRF--VCIGGELSNTKLKEWVSNVFLCPVYDLWGQTELG--IIATGPGLGFKNISSNGLS----PMLSVHPKVVNSATGEQLPNETIGRMLLKIPLCPSAASSMYNDHDRFLKTYFSKXXXXXXXXXXXXIMNDGTLQILARADDVISVSAGRICGGALEEVCLMNEDLANAAFVALEDPINGQVPFAFLEKKKDRNINNEALIIESVKLVKKEIGSFTLFHQGVVVHDLPKTKTGKILRKTLYNMLNGQDWKTPATVENPKVFEEIYSILIEA 1643
            K ++T +FG EP R V Y  +V+EA+   Q  PD  ++YNRPN +   +   RDLDW+ E+ KA  +  V + S  P++I+YTSGT G PKG++   GG+ V   + M  + GL   + WW   D GW+ G + + +GPLL G +T+LYEGK + +       R++ ++ V     APT  R ++ +D    +    +  RF  + + GE  + +  EW  NVF  PV D W QTE G  I A+  GLG       G +    P  +V   ++     ++L    +G +++K+PL P A S ++ + + F   YF K+ GYYDTMD G +  +G L +++R DDVI+V+  RI  GA+EE  L +  +A+ A V  EDP+ G VP A    +KD N   E ++ E VK V++ IG    F   V V  LPKT++GKI R  L  ++NG+ +K  +T+E+P +F  +  +L +A
Sbjct:  217 KVVVTASFGIEPGRRVEYVPLVEEALKIGQHKPDKILIYNRPNMEAVPLAPGRDLDWDEEMAKAQSHDCVPVLSEHPLYILYTSGTTGLPKGVIRPTGGYAVMLHWSMSSIYGLQPGEVWWAASDLGWVVGHSYICYGPLLHGNTTVLYEGKPVGTPDAGAYFRVLAEHGVAALFTAPTAIRAIRQQDPGAALGKQYSLTRFKTLFVAGERCDVETLEWSKNVFRVPVLDHWWQTETGSPITASCVGLGNSKTPPPGQAGKSVPGYNV---MILDDNMQKLKARCLGNIVVKLPLPPGAFSGLWKNQEAFKHLYFEKFPGYYDTMDAGYMDEEGYLYVMSRVDDVINVAGHRISAGAIEESILSHGTVADCAVVGKEDPLKGHVPLALCVLRKDINATEEQVLEEIVKHVRQNIGPVAAFRNAVFVKQLPKTRSGKIPRSALSAIVNGKPYKITSTIEDPSIFGHVEEMLKQA 686

HSP 2 Score: 78.5666 bits (192), Expect = 1.714e-98
Identity = 37/84 (44.05%), Postives = 57/84 (67.86%), Query Frame = 2 
Query:    2 PVINATEKVSYKQLLTLVENLAGVMVNKCHVQKGDRVLLYVPVTVQGIAAILACTRIGAVYVVTFGGFAPDLIAARIKHTEPKI 253
            PV N     +YK++L  V  LAGV+V K  ++KGD V++Y+P+  Q +  +LAC RIGA++ + FGGFA   +++RI H +PK+
Sbjct:  136 PVTNTKATFTYKEVLEQVSKLAGVLV-KHGIKKGDTVVIYMPMIPQAMYTMLACARIGAIHSLIFGGFASKELSSRIDHVKPKV 218
[Close]
BLAST of Propionyl-CoA synthetase vs. Ensembl Human
Match: ACSS1 (acyl-CoA synthetase short chain family member 1 [Source:HGNC Symbol;Acc:HGNC:16091])

HSP 1 Score: 182.956 bits (463), Expect = 2.348e-58
Identity = 140/482 (29.05%), Postives = 228/482 (47.30%), Query Frame = 3 
Query:  228 ELNIQSQKFIITTNFGYEPERTVNYKEIVDEAINKSQFCP--DYCIVYNRPNTKCATMIDSRDLDWETEIGKATPY-PPVALESGDPIFIIYTSGTLGEPKGLVMTVGGFVVSNKYFMKYVLGLNRRDCWWMFGDYGWISGQTTMTFGPLLIGCSTILYEGKELSSHHFTGIGR---IIDQNNVTGFSAAPTYFRFLKNKDSEKDIKPSKAQLRFVCIGGELSNTKLKEWVSNVF---LCPVYDLWGQTELGIIATGPG---LGFKNISSNGLSPMLSVHPKVVNSATGEQLPNETIGRMLLKIPLCPSAASSMYNDHDRFLKTYFSKXXXXXXXXXXXXIMNDGTLQILARADDVISVSAGRICGGALEEVCLMNEDLANAAFVALEDPINGQVPFAFLEKKKDRNINNEALIIESVKLVKKEIGSFTLFHQGVVVHDLPKTKTGKILRKTLYNMLN--GQDWKTPATVENPKVFEEIYSI 1631
             +N    K +IT N G    R V  K+IVDEA+   + CP   + +V +R + K    +   D+  E E+ K  P   P ++ S D +F++YTSG+ G PKG+V T  G+++      K V      D +    D GWI+G + + +GPL  G +++L+E    S+  +   GR    +++  +  F  APT  R L         K  ++ LR +   GE  N +  EW+  V     C + D W QTE G I   P     G + + +  + P   + P V+    G  +    +   L      P  A ++Y DH RF+  YF  Y GYY T D       G  QI  R DDVI++S  R+    +E+    +  +  +A +     I G+  FAF+   KD   +++ ++ E   +V  +I  + +  + +VV  LPKT++GK++R+ L  ++    Q+     T+E+P +  EI S+
Sbjct:  207 RINDAKCKVVITFNQGLRGGRVVELKKIVDEAV---KHCPTVQHVLVAHRTDNK--VHMGDLDVPLEQEMAKEDPVCAPESMGSEDMLFMLYTSGSTGMPKGIVHTQAGYLLYAALTHKLVFDHQPGDIFGCVADIGWITGHSYVVYGPLCNGATSVLFE----STPVYPNAGRYWETVERLKINQFYGAPTAVRLLLKYGDAWVKKYDRSSLRTLGSVGEPINCEAWEWLHRVVGDSRCTLVDTWWQTETGGICIAPRPSEEGAEILPAMAMRPFFGIVP-VLMDEKGSVVEGSNVSGALCISQAWPGMARTIYGDHQRFVDAYFKAYPGYYFTGDGAYRTEGGYYQITGRMDDVINISGHRLGTAEIEDAIADHPAVPESAVIGYPHDIKGEAAFAFI-VVKDSAGDSDVVVQELKSMVATKIAKYAVPDEILVVKRLPKTRSGKVMRRLLRKIITSEAQELGDTTTLEDPSIIAEILSV 677

HSP 2 Score: 64.3142 bits (155), Expect = 2.348e-58
Identity = 31/77 (40.26%), Postives = 50/77 (64.94%), Query Frame = 2 
Query:   23 KVSYKQLLTLVENLAGVMVNKCHVQKGDRVLLYVPVTVQGIAAILACTRIGAVYVVTFGGFAPDLIAARIKHTEPKI 253
            +++Y++LL     LA  +  +  V +GDRV +Y+PV+   +AA+LAC RIGAV+ V F GF+ + +A RI   + K+
Sbjct:  140 RITYRELLETTCRLANTL-KRHGVHRGDRVAIYMPVSPLAVAAMLACARIGAVHTVIFAGFSAESLAGRINDAKCKV 215
[Close]
BLAST of Propionyl-CoA synthetase vs. Ensembl Human
Match: ACSS2 (acyl-CoA synthetase short chain family member 2 [Source:HGNC Symbol;Acc:HGNC:15814])

HSP 1 Score: 185.652 bits (470), Expect = 8.625e-58
Identity = 153/492 (31.10%), Postives = 229/492 (46.54%), Query Frame = 3 
Query:  243 SQKFIITTNFGYEPERTVNYKEIVDEAINKSQ---FCPDYCIVYNRPNTKCATMIDSR---------------------DLDWETEIGKA-TPYPPVALESGDPIFIIYTSGTLGEPKGLVMTVGGFVVSNKYFMKYVLGLNRRDCWWMFGDYGWISGQTTMTFGPLLIGCSTILYEGKELSSHHFTGIGRIIDQNNVTGFSAAPTYFRFLKNKDSEKDIKPSKAQLRFVCIGGELSNTKLKEWVSNVF---LCPVYDLWGQTELGIIATGPGLGFKNISSNGLS-PMLSVHPKVVNSATGEQLPNETIGRMLLKIPLCPSAASSMYNDHDRFLKTYFSKXXXXXXXXXXXXIMNDGTLQILARADDVISVSAGRICGGALEEVCLMNEDLANAAFVALEDPINGQVPFAFLEKKKDRNINNEALIIESVKLVKKEIGSFTLFHQGVVVHDLPKTKTGKILRKTLYNML-NGQDWKTPATVENPKVFEEIYS 1628
            S   +ITT+  Y  E+ VN KE+ DEA+ K Q   F    CIV          M DS                      DL W   + +A     P   ++ DP+FI+YTSG+ G+PKG+V TVGG+++      KYV   +  D +W   D GWI+G + +T+GPL  G +++L+EG   +      +  I+D+  VT F  APT  R L     E   K S+A L+ +   GE  N +   W   V     CP+ D + QTE G     P  G   +     + P   V P ++N +  E    E  G ++ K P  P    ++Y +H+RF  TYF K+ GYY T D      DG   I  R DD+++VS   +    +E   + +E +A AA V    P+ G+  + F+    D +  +  L  E  K ++++IG             LPKT++GKI+R+ L  +  N  D    +TV +P V   ++S
Sbjct:  207 SCSLLITTDAFYRGEKLVNLKELADEALQKCQEKGFPVRCCIVVKHLGRAELGMGDSTSQSPPIKRSCPDVQISWNQGIDLWWHELMQEAGDECEPEWCDAEDPLFILYTSGSTGKPKGVVHTVGGYMLYVATTFKYVFDFHAEDVFWCTADIGWITGHSYVTYGPLANGATSVLFEGIP-TYPDVNRLWSIVDKYKVTKFYTAPTAIRLLMKFGDEPVTKHSRASLQVLGTVGEPINPEAWLWYHRVVGAQRCPIVDTFWQTETGGHMLTPLPGATPMKPGSATFPFFGVAPAILNESGEELE-GEAEGYLVFKQPW-PGIMRTVYGNHERFETTYFKKFPGYYVTGDGCQRDQDGYYWITGRIDDMLNVSGHLLSTAEVESALVEHEAVAEAAVVGHPHPVKGECLYCFV-TLCDGHTFSPKLTEELKKQIREKIGPIATPDYIQNAPGLPKTRSGKIMRRVLRKIAQNDHDLGDMSTVADPSVISHLFS 694

HSP 2 Score: 59.6918 bits (143), Expect = 8.625e-58
Identity = 29/72 (40.28%), Postives = 47/72 (65.28%), Query Frame = 2 
Query:   17 TEKVSYKQLLTLVENLAGVMVNKCHVQKGDRVLLYVPVTVQGIAAILACTRIGAVYVVTFGGFAPDLIAARI 232
            T +++Y QLL  V   + V+  K  +QKGDRV +Y+P+  + + A+LAC RIGA++ + F GF+ + +  RI
Sbjct:  133 TTQITYHQLLVQVCQFSNVL-RKQGIQKGDRVAIYMPMIPELVVAMLACARIGALHSIVFAGFSSESLCERI 203
[Close]
BLAST of Propionyl-CoA synthetase vs. Ensembl Human
Match: ACSS1 (acyl-CoA synthetase short chain family member 1 [Source:HGNC Symbol;Acc:HGNC:16091])

HSP 1 Score: 182.956 bits (463), Expect = 7.091e-57
Identity = 140/482 (29.05%), Postives = 228/482 (47.30%), Query Frame = 3 
Query:  228 ELNIQSQKFIITTNFGYEPERTVNYKEIVDEAINKSQFCP--DYCIVYNRPNTKCATMIDSRDLDWETEIGKATPY-PPVALESGDPIFIIYTSGTLGEPKGLVMTVGGFVVSNKYFMKYVLGLNRRDCWWMFGDYGWISGQTTMTFGPLLIGCSTILYEGKELSSHHFTGIGR---IIDQNNVTGFSAAPTYFRFLKNKDSEKDIKPSKAQLRFVCIGGELSNTKLKEWVSNVF---LCPVYDLWGQTELGIIATGPG---LGFKNISSNGLSPMLSVHPKVVNSATGEQLPNETIGRMLLKIPLCPSAASSMYNDHDRFLKTYFSKXXXXXXXXXXXXIMNDGTLQILARADDVISVSAGRICGGALEEVCLMNEDLANAAFVALEDPINGQVPFAFLEKKKDRNINNEALIIESVKLVKKEIGSFTLFHQGVVVHDLPKTKTGKILRKTLYNMLN--GQDWKTPATVENPKVFEEIYSI 1631
             +N    K +IT N G    R V  K+IVDEA+   + CP   + +V +R + K    +   D+  E E+ K  P   P ++ S D +F++YTSG+ G PKG+V T  G+++      K V      D +    D GWI+G + + +GPL  G +++L+E    S+  +   GR    +++  +  F  APT  R L         K  ++ LR +   GE  N +  EW+  V     C + D W QTE G I   P     G + + +  + P   + P V+    G  +    +   L      P  A ++Y DH RF+  YF  Y GYY T D       G  QI  R DDVI++S  R+    +E+    +  +  +A +     I G+  FAF+   KD   +++ ++ E   +V  +I  + +  + +VV  LPKT++GK++R+ L  ++    Q+     T+E+P +  EI S+
Sbjct:   86 RINDAKCKVVITFNQGLRGGRVVELKKIVDEAV---KHCPTVQHVLVAHRTDNK--VHMGDLDVPLEQEMAKEDPVCAPESMGSEDMLFMLYTSGSTGMPKGIVHTQAGYLLYAALTHKLVFDHQPGDIFGCVADIGWITGHSYVVYGPLCNGATSVLFE----STPVYPNAGRYWETVERLKINQFYGAPTAVRLLLKYGDAWVKKYDRSSLRTLGSVGEPINCEAWEWLHRVVGDSRCTLVDTWWQTETGGICIAPRPSEEGAEILPAMAMRPFFGIVP-VLMDEKGSVVEGSNVSGALCISQAWPGMARTIYGDHQRFVDAYFKAYPGYYFTGDGAYRTEGGYYQITGRMDDVINISGHRLGTAEIEDAIADHPAVPESAVIGYPHDIKGEAAFAFI-VVKDSAGDSDVVVQELKSMVATKIAKYAVPDEILVVKRLPKTRSGKVMRRLLRKIITSEAQELGDTTTLEDPSIIAEILSV 556

HSP 2 Score: 59.3066 bits (142), Expect = 7.091e-57
Identity = 30/73 (41.10%), Postives = 46/73 (63.01%), Query Frame = 2 
Query:   35 KQLLTLVENLAGVMVNKCHVQKGDRVLLYVPVTVQGIAAILACTRIGAVYVVTFGGFAPDLIAARIKHTEPKI 253
            ++LL     LA  +  +  V +GDRV +Y+PV+   +AA+LAC RIGAV+ V F GF+ + +A RI   + K+
Sbjct:   23 RELLETTCRLANTL-KRHGVHRGDRVAIYMPVSPLAVAAMLACARIGAVHTVIFAGFSAESLAGRINDAKCKV 94
[Close]
BLAST of Propionyl-CoA synthetase vs. Ensembl Celegans
Match: acs-19 (Acetyl-coenzyme A synthetase [Source:UniProtKB/TrEMBL;Acc:Q65ZB8])

HSP 1 Score: 136.346 bits (342), Expect = 1.718e-33
Identity = 123/417 (29.50%), Postives = 190/417 (45.56%), Query Frame = 3 
Query:  348 DYCIVYNRPNTKCATMIDSRDLDWETEIGKATPYPPVALESGDPIFIIYTSGTLGEPKGLVMTVGGFVVSNKYFMKYVLGLNRRDCWWMFGDYGWISGQTTMTFGPLLIGCSTILYEGKELSSHHFTGIGRIIDQNNVTGFSAAPTYFRFLKNKDSEKDIKPSKAQLRFVCIGGELSNTKLKEW------VSNVFLCPVYDLWGQTELG--IIATGPGLGFKNISSNGLSPMLSVHPKVVNSATGEQLPNETIGRMLLKIPLCPSAASSMYNDHDRFLKTYFSKXXXXXXXXXXXXIMNDGTLQILARADDVISVSAGRICGGALEEVCLMNEDLANAAFVALEDPINGQVPFAFLEKKKDRNINNEALIIESVKLVKKEIGSFTLFHQGVVVHDLPKTKTGKILRKTLYNMLNGQD 1574
            D  ++Y+    KCA  +DS  ++W              ++S DP+FI+YTSG+ G+PKG+  T  G++       KY       D +W   D GWI+G + + +GPL+ G   I YEG        + +  + D+  VT    +PT  R L    ++     S+  L+ +   GE  N     W      +SNV    + D + QTE G  +I   PG       +  + P     P V+  A G  +     G +       P     +Y D  RF+KTY + ++GYY T D      DG L I  R DD+++VS   +    +E   + +E +A AA VA    I G  P+AF+       I NE L+ E  KLV+++IG+  +         LPKT++GK+ R+ L  +  G +
Sbjct:  252 DITVIYDSEMLKCAG-VDS-PVEW--------------MDSEDPLFILYTSGSTGKPKGIQHTTAGYMTYAYATTKYTFDAQEDDVYWCTADCGWITGHSYLLYGPLMNGLKGIWYEGVPTYPTP-SRMWDVTDKYGVTKLYTSPTAARALMALGNQWLESSSRKTLKVIGTVGEPINPAAWMWLYKQVGLSNV---SIVDTYWQTETGGHMITCLPGATPMKPGAAAM-PFFGASP-VLLDAEGRVIEGPGEGSLCFD-RAWPGMMRGIYGDEQRFVKTYLAPFNGYYFTGDGARRDEDGYLWITGRVDDLMNVSGHLLSTAEIESALVAHEKVAEAAVVAAPHDIKGSFPYAFVTLNVGERI-NEKLVAELKKLVREKIGALAVPDVIQEAPGLPKTRSGKVTRRILRKIAEGSE 644

HSP 2 Score: 50.8322 bits (120), Expect = 2.385e-6
Identity = 28/73 (38.36%), Postives = 45/73 (61.64%), Query Frame = 2 
Query:   17 TEKVSYKQLLTLVENLAGVMVNKCH-VQKGDRVLLYVPVTVQGIAAILACTRIGAVYVVTFGGFAPDLIAARI 232
            T   +Y +L   V   + V+  + H V++GD V LY+P+  +   A+LAC RIGA++ V F GF+ + +AAR+
Sbjct:  114 TSTWTYNELHAQVVQFSAVL--RSHGVKRGDVVALYLPMIPELAVAMLACARIGAMHSVVFAGFSAESLAARV 184
[Close]
BLAST of Propionyl-CoA synthetase vs. Ensembl Celegans
Match: acs-19 (Acetyl-coenzyme A synthetase [Source:UniProtKB/TrEMBL;Acc:Q65ZB8])

HSP 1 Score: 135.961 bits (341), Expect = 2.088e-33
Identity = 124/417 (29.74%), Postives = 190/417 (45.56%), Query Frame = 3 
Query:  348 DYCIVYNRPNTKCATMIDSRDLDWETEIGKATPYPPVALESGDPIFIIYTSGTLGEPKGLVMTVGGFVVSNKYFMKYVLGLNRRDCWWMFGDYGWISGQTTMTFGPLLIGCSTILYEGKELSSHHFTGIGRIIDQNNVTGFSAAPTYFRFLKNKDSEKDIKPSKAQLRFVCIGGELSNTKLKEW------VSNVFLCPVYDLWGQTELG--IIATGPGLGFKNISSNGLSPMLSVHPKVVNSATGEQLPNETIGRMLLKIPLCPSAASSMYNDHDRFLKTYFSKXXXXXXXXXXXXIMNDGTLQILARADDVISVSAGRICGGALEEVCLMNEDLANAAFVALEDPINGQVPFAFLEKKKDRNINNEALIIESVKLVKKEIGSFTLFHQGVVVHDLPKTKTGKILRKTLYNMLNGQD 1574
            D  ++Y+    KCA  +DS  ++W              ++S DP+FI+YTSG+ G+PKG+  T  G++       KY       D +W   D GWI+G + + +GPL+ G   I YEG        + +  + D+  VT    +PT  R L    ++     S+  L+ +   GE  N     W      +SNV +   Y  W QTE G  +I   PG       +  + P     P V+  A G  +     G +       P     +Y D  RF+KTY + ++GYY T D      DG L I  R DD+++VS   +    +E   + +E +A AA VA    I G  P+AF+       I NE L+ E  KLV+++IG+  +         LPKT++GK+ R+ L  +  G +
Sbjct:  262 DITVIYDSEMLKCAG-VDS-PVEW--------------MDSEDPLFILYTSGSTGKPKGIQHTTAGYMTYAYATTKYTFDAQEDDVYWCTADCGWITGHSYLLYGPLMNGLKGIWYEGVPTYPTP-SRMWDVTDKYGVTKLYTSPTAARALMALGNQWLESSSRKTLKVIGTVGEPINPAAWMWLYKQVGLSNVSIVDTY--W-QTETGGHMITCLPGATPMKPGAAAM-PFFGASP-VLLDAEGRVIEGPGEGSLCFD-RAWPGMMRGIYGDEQRFVKTYLAPFNGYYFTGDGARRDEDGYLWITGRVDDLMNVSGHLLSTAEIESALVAHEKVAEAAVVAAPHDIKGSFPYAFVTLNVGERI-NEKLVAELKKLVREKIGALAVPDVIQEAPGLPKTRSGKVTRRILRKIAEGSE 654

HSP 2 Score: 50.8322 bits (120), Expect = 2.462e-6
Identity = 28/73 (38.36%), Postives = 45/73 (61.64%), Query Frame = 2 
Query:   17 TEKVSYKQLLTLVENLAGVMVNKCH-VQKGDRVLLYVPVTVQGIAAILACTRIGAVYVVTFGGFAPDLIAARI 232
            T   +Y +L   V   + V+  + H V++GD V LY+P+  +   A+LAC RIGA++ V F GF+ + +AAR+
Sbjct:  124 TSTWTYNELHAQVVQFSAVL--RSHGVKRGDVVALYLPMIPELAVAMLACARIGAMHSVVFAGFSAESLAARV 194
[Close]
BLAST of Propionyl-CoA synthetase vs. Ensembl Celegans
Match: acs-19 (Acetyl-coenzyme A synthetase [Source:UniProtKB/TrEMBL;Acc:Q65ZB8])

HSP 1 Score: 135.961 bits (341), Expect = 2.088e-33
Identity = 124/417 (29.74%), Postives = 190/417 (45.56%), Query Frame = 3 
Query:  348 DYCIVYNRPNTKCATMIDSRDLDWETEIGKATPYPPVALESGDPIFIIYTSGTLGEPKGLVMTVGGFVVSNKYFMKYVLGLNRRDCWWMFGDYGWISGQTTMTFGPLLIGCSTILYEGKELSSHHFTGIGRIIDQNNVTGFSAAPTYFRFLKNKDSEKDIKPSKAQLRFVCIGGELSNTKLKEW------VSNVFLCPVYDLWGQTELG--IIATGPGLGFKNISSNGLSPMLSVHPKVVNSATGEQLPNETIGRMLLKIPLCPSAASSMYNDHDRFLKTYFSKXXXXXXXXXXXXIMNDGTLQILARADDVISVSAGRICGGALEEVCLMNEDLANAAFVALEDPINGQVPFAFLEKKKDRNINNEALIIESVKLVKKEIGSFTLFHQGVVVHDLPKTKTGKILRKTLYNMLNGQD 1574
            D  ++Y+    KCA  +DS  ++W              ++S DP+FI+YTSG+ G+PKG+  T  G++       KY       D +W   D GWI+G + + +GPL+ G   I YEG        + +  + D+  VT    +PT  R L    ++     S+  L+ +   GE  N     W      +SNV +   Y  W QTE G  +I   PG       +  + P     P V+  A G  +     G +       P     +Y D  RF+KTY + ++GYY T D      DG L I  R DD+++VS   +    +E   + +E +A AA VA    I G  P+AF+       I NE L+ E  KLV+++IG+  +         LPKT++GK+ R+ L  +  G +
Sbjct:  262 DITVIYDSEMLKCAG-VDS-PVEW--------------MDSEDPLFILYTSGSTGKPKGIQHTTAGYMTYAYATTKYTFDAQEDDVYWCTADCGWITGHSYLLYGPLMNGLKGIWYEGVPTYPTP-SRMWDVTDKYGVTKLYTSPTAARALMALGNQWLESSSRKTLKVIGTVGEPINPAAWMWLYKQVGLSNVSIVDTY--W-QTETGGHMITCLPGATPMKPGAAAM-PFFGASP-VLLDAEGRVIEGPGEGSLCFD-RAWPGMMRGIYGDEQRFVKTYLAPFNGYYFTGDGARRDEDGYLWITGRVDDLMNVSGHLLSTAEIESALVAHEKVAEAAVVAAPHDIKGSFPYAFVTLNVGERI-NEKLVAELKKLVREKIGALAVPDVIQEAPGLPKTRSGKVTRRILRKIAEGSE 654

HSP 2 Score: 50.8322 bits (120), Expect = 2.462e-6
Identity = 28/73 (38.36%), Postives = 45/73 (61.64%), Query Frame = 2 
Query:   17 TEKVSYKQLLTLVENLAGVMVNKCH-VQKGDRVLLYVPVTVQGIAAILACTRIGAVYVVTFGGFAPDLIAARI 232
            T   +Y +L   V   + V+  + H V++GD V LY+P+  +   A+LAC RIGA++ V F GF+ + +AAR+
Sbjct:  124 TSTWTYNELHAQVVQFSAVL--RSHGVKRGDVVALYLPMIPELAVAMLACARIGAMHSVVFAGFSAESLAARV 194
[Close]
BLAST of Propionyl-CoA synthetase vs. Ensembl Celegans
Match: mec-18 (pep chromosome:WBcel235:X:4271169:4274229:-1 gene:WBGene00003179.1 transcript:C52B9.9.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:mec-18)

HSP 1 Score: 58.151 bits (139), Expect = 1.149e-8
Identity = 88/381 (23.10%), Postives = 157/381 (41.21%), Query Frame = 3 
Query:  453 PVALESG-DPIFIIYTSGTLGEPKGLVMTVGGFVVSNKYFMKYVLG-LNRRDCWWMFGDYGWISGQTTMTFGPLLIGCSTILYEGKELSSHHFTGIGRIIDQNNVTGFSAAPTYFRFLKNKDSEKDIKPSKAQLRFV-CIGGELSNTKLKEWVSNVFLCPVYDLWGQTELGIIATGPGLGFKNISSNGLSPMLSVHPKVVNSATGEQLPNETIGRMLL---KIPLC----PSAASSMYNDHDRFLKT----YFSKXXXXXXXXXXXXIMNDGTLQILARADDVISVSAGRICGGALEEVCLMNEDLANAAFVALEDPINGQVPFAFLEKKKDRNINNEALIIESVKLVKKEIGSFTLFHQGV-VVHDLPKTKTGKILRKTL 1550
            P+   SG +P+ I++TSGT G  K   ++    +++ +     + G +  ++ + +      I G  +  +  L+ G S  LY   + S+  F      +  N +      P    ++       D K     LR V C G  + +       S + +      +G TELG I T      + I S G +P+  +  KVVN  T +       G++++   ++  C    P A S ++ D   F+KT    ++ +                G + +L R  D+I      IC   +E V   +  + + A V  +D + G+VP AF+ K     +   A   E  + V  +I +F     GV  + ++P++  GKILR+ L
Sbjct:  231 PIQQVSGQNPLLILFTSGTTGLAKAAELSHRSLIINIQQISLPLYGPVQTKERFLLPLSIAHIYGIVSAYYA-LINGAS--LYLISKQSNRLFM---ETLVNNQINVMHITPAIVHWMATDAIVDDYK--TPNLRSVLCAGAPIDSNSAAAMKSRLNIKDFRQSFGMTELGGICTMSPYLDEKIESVG-NPLPGMLFKVVNWETKQLCLPRQPGQIIVLGPQVSPCYYKNPKATSELF-DATGFVKTGDAGFYDEV---------------GRIYVLDRIKDIIKCKGTMICPSEVELVLRAHAGIDDCAVVGRQDHVTGEVPAAFVVKNAQHPLLASA---EVRQYVSGKIATFKELRGGVFFISEIPRSVCGKILRRNL 583
[Close]
BLAST of Propionyl-CoA synthetase vs. Ensembl Celegans
Match: acs-6 (Fatty Acid CoA Synthetase family [Source:UniProtKB/TrEMBL;Acc:Q23404])

HSP 1 Score: 50.8322 bits (120), Expect = 2.552e-6
Identity = 90/400 (22.50%), Postives = 157/400 (39.25%), Query Frame = 3 
Query:  441 TPYPPVALE-SGDPIFII-YTSGTLGEPKGLVMT---VGGFVVSNKYFMKYVLG---LNRRDCWW------MFGDYGWISGQTTMTFGPLLIGCSTILYEGKELSSHHFTGIGRIIDQNNVTGFSA-----APTYFRFLKNKDSEKDIKPSKAQLRFVCIGGELSNTKLKEWVS----NVFLCPVYDLWGQTELGIIATGPGLGFKNISSNGLSPMLSVHPKVVNSATGEQLPNETIGRMLLKIPLCPSA----ASSMYNDHDRFLKTYFSKXXXXXXXXXXXXIMNDGTLQILARADDVISVSAGRICGGALEEVCLMNEDLANAAFVALEDPINGQVPFAFLEKKKDRNINNEALIIESVKLVKKEIGSFTLFHQGVVVHDLPKTKTGKILRKTLYNM 1559
            TP  P+ +  S D I ++ Y+SGT G PKG  +T   +G  +   K  ++  +      +    W      +   +    G  TM F  +L+G + I+          F     I+  N +  +        P    FL  KD    I  +   L+ +   G  +  +L E VS    N +LC  Y   G TE+    T P     N      +   +   K+++    E     T+G++  + P           +   D D FL T            D G I + G + +  R  ++I V+  ++    +E+V L++  + + A + + D   G+ P A++  KKD  +    L     + V++++ S+        +  +PK  +GKI RK L  M
Sbjct:  171 TPDQPINVNVSMDSIALLPYSSGTTGRPKGCQLTHRNIGAMLDVAKAHLETDVAPAMFGKEKATWHKEHTVLLLPWYHAYGLNTM-FETILLGMTGIV----------FKKFDTIVMLNRIKFYKVKLAWLVPPMLIFLA-KDPMVPIFNTAPFLKVIMSAGATAGKQLCEEVSKRFPNAWLCQAY---GMTEMVQFTTIPRFEDGNCFETVGNLASTYELKILDKEKKEITTINTVGQLCFRGPTVMKGYLKREEADIIDKDGFLLT-----------GDLGSIDDKGRIHVTGRIKELIKVNGMQVPPVEIEDVLLLHPKVKDCAVIGVPDEHKGESPKAYI-VKKDHTLTEAEL----TEFVRQKLSSYKWIDTYEFIDSIPKLPSGKIQRKKLKKM 539
[Close]
BLAST of Propionyl-CoA synthetase vs. Ensembl Fly
Match: CG6432 (gene:FBgn0039184 transcript:FBtr0346110)

HSP 1 Score: 249.98 bits (637), Expect = 8.323e-80
Identity = 159/486 (32.72%), Postives = 258/486 (53.09%), Query Frame = 3 
Query:  249 KFIITTNFGYEPERTVNYKEIVDEAINKSQF-CPDYCIVYNRPNTKC-ATMIDS-RDLDWETEIGKATPYPPVA---LESGDPIFIIYTSGTLGEPKGLVMTVGGFVVSNKYFMKYVLGLNRRDCWWMFGDYGWISGQTTMTFGPLLIGCSTILYEGKELSSHHFTGIGRIIDQNNVTGFSAAPTYFRFLKNKDSEKDI--KPSKAQLRFVCIGGELSNTKLKEWVSNVFLCPVYDLWGQTELGIIATGPGLGF-KNIS----SNGLSPMLSVHPKVVNSATGEQLPNETIGRMLLKIPLCPSAASSMYNDHDRFLKTYFSKXXXXXXXXXXXXIMNDGTLQILARADDVISVSAGRICGGALEEVCLMNEDLANAAFVALEDPINGQVPFAFLEKKKDRNINNEALIIESVKLVKKEIGSFTLFHQGVVVHDLPKTKTGKILRKTLYNMLNGQDWKTPATVENPKVFEEIYSILIEAGYKASLPN 1667
            K +I +N G EP + V Y +I+  AI+ S++  P   I++ R N     T +D   D+ W   +  A    P+A   +E+ DP++I+YTSGT  +PKG++ T+GG +V+  Y ++ + G+N    WW   D GW+ G + + +GPL +G ++++YEGK   +       RIIDQ  V    + PT FR ++  D +     + S   LR + I GE  + + K W+   F  PV + W QTE G   T   LGF +N+S    S GL P++    K++  A G +     +GR+ LK+PL P   +++Y + + F   YF K+ GYYDTMD G     G + + AR DDVI+V+  R+   +LE+  L + D+ + A   + +   GQVP       ++    +  L  E +KL++  +G    F     V++LP+T++GK +RK + +    +    PAT+++  VF EI   L + GY  + P+
Sbjct:  183 KLVIASNVGVEPGKVVPYLDILHSAISMSRWRPPQRNIIFRRDNVSPDTTKLDPLTDVLWSDILKMAEGERPIACVPIEANDPLYILYTSGTTDKPKGVLRTIGGHLVALVYTLRTLYGINPGHTWWAASDMGWVVGHSYICYGPLCLGATSVMYEGKPDRTPDPGQYFRIIDQYQVRSIFSVPTSFRVIRRADPDISYGRQYSMKSLRAIFIAGEHCDYETKSWIEKTFKVPVLNHWWQTETGSAVTATCLGFQQNLSPPTYSTGL-PLMGYDVKILK-ADGSEAQTSELGRIALKLPLPPGNMATLYKNEELFRTLYFQKFPGYYDTMDAGYKDERGYIFVTARDDDVINVAGHRLSTSSLEDAVLRHPDVVDVAVFGVPEATKGQVPLCLYIPVENCKKTDAKLSTEIIKLIRDVVGPIAAFRLVTSVNNLPRTRSGKTMRKAMADFARNERVVLPATIDDASVFIEIRRALNQLGYAMTAPD 666

HSP 2 Score: 68.1662 bits (165), Expect = 8.323e-80
Identity = 35/84 (41.67%), Postives = 55/84 (65.48%), Query Frame = 2 
Query:    2 PVINATEKVSYKQLLTLVENLAGVMVNKCHVQKGDRVLLYVPVTVQGIAAILACTRIGAVYVVTFGGFAPDLIAARIKHTEPKI 253
            P+     +V+Y++L   +  LAG +  K  V KGDRV++Y+P+  + I A+LA  R+GA++ V FGGFA   + +RI+H EPK+
Sbjct:  102 PLTGTLRRVTYQELYDQIVLLAGGLA-KLGVVKGDRVVIYMPLIPETIIAMLAIVRLGAIHSVVFGGFAARELCSRIEHVEPKL 184
[Close]
BLAST of Propionyl-CoA synthetase vs. Ensembl Fly
Match: CG6432 (gene:FBgn0039184 transcript:FBtr0084606)

HSP 1 Score: 249.98 bits (637), Expect = 8.323e-80
Identity = 159/486 (32.72%), Postives = 258/486 (53.09%), Query Frame = 3 
Query:  249 KFIITTNFGYEPERTVNYKEIVDEAINKSQF-CPDYCIVYNRPNTKC-ATMIDS-RDLDWETEIGKATPYPPVA---LESGDPIFIIYTSGTLGEPKGLVMTVGGFVVSNKYFMKYVLGLNRRDCWWMFGDYGWISGQTTMTFGPLLIGCSTILYEGKELSSHHFTGIGRIIDQNNVTGFSAAPTYFRFLKNKDSEKDI--KPSKAQLRFVCIGGELSNTKLKEWVSNVFLCPVYDLWGQTELGIIATGPGLGF-KNIS----SNGLSPMLSVHPKVVNSATGEQLPNETIGRMLLKIPLCPSAASSMYNDHDRFLKTYFSKXXXXXXXXXXXXIMNDGTLQILARADDVISVSAGRICGGALEEVCLMNEDLANAAFVALEDPINGQVPFAFLEKKKDRNINNEALIIESVKLVKKEIGSFTLFHQGVVVHDLPKTKTGKILRKTLYNMLNGQDWKTPATVENPKVFEEIYSILIEAGYKASLPN 1667
            K +I +N G EP + V Y +I+  AI+ S++  P   I++ R N     T +D   D+ W   +  A    P+A   +E+ DP++I+YTSGT  +PKG++ T+GG +V+  Y ++ + G+N    WW   D GW+ G + + +GPL +G ++++YEGK   +       RIIDQ  V    + PT FR ++  D +     + S   LR + I GE  + + K W+   F  PV + W QTE G   T   LGF +N+S    S GL P++    K++  A G +     +GR+ LK+PL P   +++Y + + F   YF K+ GYYDTMD G     G + + AR DDVI+V+  R+   +LE+  L + D+ + A   + +   GQVP       ++    +  L  E +KL++  +G    F     V++LP+T++GK +RK + +    +    PAT+++  VF EI   L + GY  + P+
Sbjct:  183 KLVIASNVGVEPGKVVPYLDILHSAISMSRWRPPQRNIIFRRDNVSPDTTKLDPLTDVLWSDILKMAEGERPIACVPIEANDPLYILYTSGTTDKPKGVLRTIGGHLVALVYTLRTLYGINPGHTWWAASDMGWVVGHSYICYGPLCLGATSVMYEGKPDRTPDPGQYFRIIDQYQVRSIFSVPTSFRVIRRADPDISYGRQYSMKSLRAIFIAGEHCDYETKSWIEKTFKVPVLNHWWQTETGSAVTATCLGFQQNLSPPTYSTGL-PLMGYDVKILK-ADGSEAQTSELGRIALKLPLPPGNMATLYKNEELFRTLYFQKFPGYYDTMDAGYKDERGYIFVTARDDDVINVAGHRLSTSSLEDAVLRHPDVVDVAVFGVPEATKGQVPLCLYIPVENCKKTDAKLSTEIIKLIRDVVGPIAAFRLVTSVNNLPRTRSGKTMRKAMADFARNERVVLPATIDDASVFIEIRRALNQLGYAMTAPD 666

HSP 2 Score: 68.1662 bits (165), Expect = 8.323e-80
Identity = 35/84 (41.67%), Postives = 55/84 (65.48%), Query Frame = 2 
Query:    2 PVINATEKVSYKQLLTLVENLAGVMVNKCHVQKGDRVLLYVPVTVQGIAAILACTRIGAVYVVTFGGFAPDLIAARIKHTEPKI 253
            P+     +V+Y++L   +  LAG +  K  V KGDRV++Y+P+  + I A+LA  R+GA++ V FGGFA   + +RI+H EPK+
Sbjct:  102 PLTGTLRRVTYQELYDQIVLLAGGLA-KLGVVKGDRVVIYMPLIPETIIAMLAIVRLGAIHSVVFGGFAARELCSRIEHVEPKL 184
[Close]
BLAST of Propionyl-CoA synthetase vs. Ensembl Fly
Match: AcCoAS (gene:FBgn0012034 transcript:FBtr0333126)

HSP 1 Score: 176.792 bits (447), Expect = 5.531e-56
Identity = 137/482 (28.42%), Postives = 234/482 (48.55%), Query Frame = 3 
Query:  249 KFIITTNFGYEPERTVNYKEIVDEAINKSQ---FCPDYCIVYN--RPNTKCAT--------MIDSRDLDWETEIGKATPYP-PVALESGDPIFIIYTSGTLGEPKGLVMTVGGFVVSNKYFMKYVLGLNRRDCWWMFGDYGWISGQTTMTFGPLLIGCSTILYEGKELSSHHFTGIGR---IIDQNNVTGFSAAPTYFRFLKNKDSEKDIKPSKAQLRFVCIGGELSNTKLKEWVSNVF---LCPVYDLWGQTELGIIATGPGLGFKNISSNGLS-PMLSVHPKVVNSATGEQLPNETIGRMLLKIPLCPSAASSMYNDHDRFLKTYFSKXXXXXXXXXXXXIMNDGTLQILARADDVISVSAGRICGGALEEVCLMNEDLANAAFVALEDPINGQVPFAFLEKKKDRNINNEALIIESVKLVKKEIGSFTLFHQGVVVHDLPKTKTGKILRKTLYNM-LNGQDWKTPATVENPKVFEEIYS 1628
            K +IT +  +  E+ +  K + D A+ K +      + CIV +  +  T C            D RD  W  E+    P   P  +++ DP+F++YTSG+ G+PKG++ T  G+++      K V      D +W  GD GWI+G T + +GPL  G +++++EG       F G  R   +ID+  VT F  APT  R L        +K + + L+ +   GE  N +   W         C + D + QTE G     P  G   +     S P   V P +++   G ++  E  G ++   P  P    ++YN+H+RF  TYFSK+ GYY T D      DG L I  R DD+++VS   +    +E V   +  +A +A V+   P+ G+  + F+   ++  + ++ LI +  K+V++ IG F +         LPKT++GKI+R+ L  + +N ++    +T+ + ++ E++++
Sbjct:  189 KLLITADGAWRGEKPLYLKALCDTALEKVEEMGHSVEKCIVVSHLKRVTPCQPDHVEEEIPWTDDRDYWWHEEMEDKEPACYPEWMDAEDPLFMLYTSGSTGKPKGVLHTTAGYLLYAATTFKIVFDYKPGDIYWCTGDVGWITGHTYVVYGPLANGATSVIFEGTPF----FPGNDRYWSVIDKYKVTQFYTAPTAIRALMKFGEGPVLKHNLSGLKVLGSVGEPINPEAWLWYYKYIGKEQCSIVDTFWQTETGGHVITPLPGATPMKPGSASFPFFGVKPTLLDEC-GIEIKGEGEGYLVFSQPW-PGMMRTLYNNHERFEDTYFSKFPGYYCTGDGARRDADGYLWITGRVDDMLNVSGHLMSTAEVESVLTEHPRVAESAVVSRPHPVKGECLYCFITPNEN-EVFDQKLISDLKKMVRERIGPFAMPDVIQNAPGLPKTRSGKIMRRVLRKIAVNDRNVGDTSTLADEQIVEQLFA 663

HSP 2 Score: 62.003 bits (149), Expect = 5.531e-56
Identity = 31/75 (41.33%), Postives = 51/75 (68.00%), Query Frame = 2 
Query:   11 NATEKVSYKQLLTLVENLAGVMVNKCH-VQKGDRVLLYVPVTVQGIAAILACTRIGAVYVVTFGGFAPDLIAARI 232
            + +  ++Y++LL  V   A V+  K H ++KGDRV +Y+P+ ++   A+LAC RIGAV+ + F GF+PD +A R+
Sbjct:  111 DYSRGLTYRKLLEEVCRFANVL--KDHGIRKGDRVSIYMPMILELPIAMLACARIGAVHSIVFAGFSPDSLAERM 183
[Close]
BLAST of Propionyl-CoA synthetase vs. Ensembl Fly
Match: AcCoAS (gene:FBgn0012034 transcript:FBtr0078413)

HSP 1 Score: 176.792 bits (447), Expect = 5.531e-56
Identity = 137/482 (28.42%), Postives = 234/482 (48.55%), Query Frame = 3 
Query:  249 KFIITTNFGYEPERTVNYKEIVDEAINKSQ---FCPDYCIVYN--RPNTKCAT--------MIDSRDLDWETEIGKATPYP-PVALESGDPIFIIYTSGTLGEPKGLVMTVGGFVVSNKYFMKYVLGLNRRDCWWMFGDYGWISGQTTMTFGPLLIGCSTILYEGKELSSHHFTGIGR---IIDQNNVTGFSAAPTYFRFLKNKDSEKDIKPSKAQLRFVCIGGELSNTKLKEWVSNVF---LCPVYDLWGQTELGIIATGPGLGFKNISSNGLS-PMLSVHPKVVNSATGEQLPNETIGRMLLKIPLCPSAASSMYNDHDRFLKTYFSKXXXXXXXXXXXXIMNDGTLQILARADDVISVSAGRICGGALEEVCLMNEDLANAAFVALEDPINGQVPFAFLEKKKDRNINNEALIIESVKLVKKEIGSFTLFHQGVVVHDLPKTKTGKILRKTLYNM-LNGQDWKTPATVENPKVFEEIYS 1628
            K +IT +  +  E+ +  K + D A+ K +      + CIV +  +  T C            D RD  W  E+    P   P  +++ DP+F++YTSG+ G+PKG++ T  G+++      K V      D +W  GD GWI+G T + +GPL  G +++++EG       F G  R   +ID+  VT F  APT  R L        +K + + L+ +   GE  N +   W         C + D + QTE G     P  G   +     S P   V P +++   G ++  E  G ++   P  P    ++YN+H+RF  TYFSK+ GYY T D      DG L I  R DD+++VS   +    +E V   +  +A +A V+   P+ G+  + F+   ++  + ++ LI +  K+V++ IG F +         LPKT++GKI+R+ L  + +N ++    +T+ + ++ E++++
Sbjct:  189 KLLITADGAWRGEKPLYLKALCDTALEKVEEMGHSVEKCIVVSHLKRVTPCQPDHVEEEIPWTDDRDYWWHEEMEDKEPACYPEWMDAEDPLFMLYTSGSTGKPKGVLHTTAGYLLYAATTFKIVFDYKPGDIYWCTGDVGWITGHTYVVYGPLANGATSVIFEGTPF----FPGNDRYWSVIDKYKVTQFYTAPTAIRALMKFGEGPVLKHNLSGLKVLGSVGEPINPEAWLWYYKYIGKEQCSIVDTFWQTETGGHVITPLPGATPMKPGSASFPFFGVKPTLLDEC-GIEIKGEGEGYLVFSQPW-PGMMRTLYNNHERFEDTYFSKFPGYYCTGDGARRDADGYLWITGRVDDMLNVSGHLMSTAEVESVLTEHPRVAESAVVSRPHPVKGECLYCFITPNEN-EVFDQKLISDLKKMVRERIGPFAMPDVIQNAPGLPKTRSGKIMRRVLRKIAVNDRNVGDTSTLADEQIVEQLFA 663

HSP 2 Score: 62.003 bits (149), Expect = 5.531e-56
Identity = 31/75 (41.33%), Postives = 51/75 (68.00%), Query Frame = 2 
Query:   11 NATEKVSYKQLLTLVENLAGVMVNKCH-VQKGDRVLLYVPVTVQGIAAILACTRIGAVYVVTFGGFAPDLIAARI 232
            + +  ++Y++LL  V   A V+  K H ++KGDRV +Y+P+ ++   A+LAC RIGAV+ + F GF+PD +A R+
Sbjct:  111 DYSRGLTYRKLLEEVCRFANVL--KDHGIRKGDRVSIYMPMILELPIAMLACARIGAVHSIVFAGFSPDSLAERM 183
[Close]
BLAST of Propionyl-CoA synthetase vs. Ensembl Fly
Match: AcCoAS (gene:FBgn0012034 transcript:FBtr0078414)

HSP 1 Score: 176.792 bits (447), Expect = 5.021e-50
Identity = 137/482 (28.42%), Postives = 234/482 (48.55%), Query Frame = 3 
Query:  249 KFIITTNFGYEPERTVNYKEIVDEAINKSQ---FCPDYCIVYN--RPNTKCAT--------MIDSRDLDWETEIGKATPYP-PVALESGDPIFIIYTSGTLGEPKGLVMTVGGFVVSNKYFMKYVLGLNRRDCWWMFGDYGWISGQTTMTFGPLLIGCSTILYEGKELSSHHFTGIGR---IIDQNNVTGFSAAPTYFRFLKNKDSEKDIKPSKAQLRFVCIGGELSNTKLKEWVSNVF---LCPVYDLWGQTELGIIATGPGLGFKNISSNGLS-PMLSVHPKVVNSATGEQLPNETIGRMLLKIPLCPSAASSMYNDHDRFLKTYFSKXXXXXXXXXXXXIMNDGTLQILARADDVISVSAGRICGGALEEVCLMNEDLANAAFVALEDPINGQVPFAFLEKKKDRNINNEALIIESVKLVKKEIGSFTLFHQGVVVHDLPKTKTGKILRKTLYNM-LNGQDWKTPATVENPKVFEEIYS 1628
            K +IT +  +  E+ +  K + D A+ K +      + CIV +  +  T C            D RD  W  E+    P   P  +++ DP+F++YTSG+ G+PKG++ T  G+++      K V      D +W  GD GWI+G T + +GPL  G +++++EG       F G  R   +ID+  VT F  APT  R L        +K + + L+ +   GE  N +   W         C + D + QTE G     P  G   +     S P   V P +++   G ++  E  G ++   P  P    ++YN+H+RF  TYFSK+ GYY T D      DG L I  R DD+++VS   +    +E V   +  +A +A V+   P+ G+  + F+   ++  + ++ LI +  K+V++ IG F +         LPKT++GKI+R+ L  + +N ++    +T+ + ++ E++++
Sbjct:   43 KLLITADGAWRGEKPLYLKALCDTALEKVEEMGHSVEKCIVVSHLKRVTPCQPDHVEEEIPWTDDRDYWWHEEMEDKEPACYPEWMDAEDPLFMLYTSGSTGKPKGVLHTTAGYLLYAATTFKIVFDYKPGDIYWCTGDVGWITGHTYVVYGPLANGATSVIFEGTPF----FPGNDRYWSVIDKYKVTQFYTAPTAIRALMKFGEGPVLKHNLSGLKVLGSVGEPINPEAWLWYYKYIGKEQCSIVDTFWQTETGGHVITPLPGATPMKPGSASFPFFGVKPTLLDEC-GIEIKGEGEGYLVFSQPW-PGMMRTLYNNHERFEDTYFSKFPGYYCTGDGARRDADGYLWITGRVDDMLNVSGHLMSTAEVESVLTEHPRVAESAVVSRPHPVKGECLYCFITPNEN-EVFDQKLISDLKKMVRERIGPFAMPDVIQNAPGLPKTRSGKIMRRVLRKIAVNDRNVGDTSTLADEQIVEQLFA 517

HSP 2 Score: 41.5874 bits (96), Expect = 5.021e-50
Identity = 17/37 (45.95%), Postives = 27/37 (72.97%), Query Frame = 2 
Query:  122 VPVTVQGIAAILACTRIGAVYVVTFGGFAPDLIAARI 232
            +P+ ++   A+LAC RIGAV+ + F GF+PD +A R+
Sbjct:    1 MPMILELPIAMLACARIGAVHSIVFAGFSPDSLAERM 37
[Close]
BLAST of Propionyl-CoA synthetase vs. Ensembl Zebrafish
Match: acss3 (acyl-CoA synthetase short chain family member 3 [Source:ZFIN;Acc:ZDB-GENE-060503-816])

HSP 1 Score: 285.419 bits (729), Expect = 3.882e-91
Identity = 169/472 (35.81%), Postives = 268/472 (56.78%), Query Frame = 3 
Query:  249 KFIITTNFGYEPERTVNYKEIVDEAINKSQFCPDYCIVYNRPNTKCATMIDSRDLDWETEIGKATPYPPVALESGDPIFIIYTSGTLGEPKGLVMTVGGFVVSNKYFMKYVLGLNRRDCWWMFGDYGWISGQTTMTFGPLLIGCSTILYEGKELSSHHFTGIGRIIDQNNVTGFSAAPTYFRFLKNKDSEKD---IKPSKAQLRFVCIGGELSNTKLKEWVSNVFLCPVYDLWGQTELG--IIATGPGLGFKNISSNGLS--PMLSVHPKVVNSATGEQLPNETIGRMLLKIPLCPSAASSMYNDHDRFLKTYFSKXXXXXXXXXXXXIMNDGTLQILARADDVISVSAGRICGGALEEVCLMNEDLANAAFVALEDPINGQVPFAFLEKKKDRNINNEALIIESVKLVKKEIGSFTLFHQGVVVHDLPKTKTGKILRKTLYNMLNGQDWKTPATVENPKVFEEIYSILIEA 1643
            K ++T +FG EP R V Y  +V++A+  S   P   ++YNRP+ +  +      LDWE E+  A P+  V++ +  P++++YTSGT G PKG+V   GG+ V   + M  V GL   + WW   D GW+ G + + +GPLL G +T+LYEGK + +       R++ ++       APT  R ++ +D + +   + P   +LR + + GE  + +  EW    F  PV D W QTE G  I A+  GLG       G +  P+   +  V++    +Q+   T+G +++K+PL P AA S++ +H  F + YF+K+ GYYDTMD G +  +G L I+AR+DDVI+V+  R+  GALEE  L++  + + A V LED + G VP A    + D   + + L+   VKLV+  IG    F +   V  LPKT++GKI R +L N++N + +K   T+E+P+VF++I  +L E+
Sbjct:  157 KLLVTASFGIEPGRLVEYIPLVEKALELSTHKPHKVLIYNRPSMEKVSTRPDLTLDWEEEMSTARPHDCVSVSAHHPLYVLYTSGTTGSPKGVVRDSGGYAVMLNWTMSNVYGLAPGEVWWAASDLGWVVGHSYICYGPLLHGNTTVLYEGKPVGTPDPGAFFRVMSEHGTACMFTAPTAIRAIRQQDPQAEHGKLYPLN-RLRNLFVAGERCDIETLEWAKKSFGVPVLDHWWQTESGSSITASCVGLGCSLAPPPGTAGKPVPGYNVTVIDDDM-QQVKPRTLGNIVVKLPLPPGAALSLWQNHMLFKELYFTKFTGYYDTMDAGFVDEEGFLYIMARSDDVINVAGHRLSTGALEESVLLHPAVVDCAVVGLEDSLKGHVPLALCVLRNDCKESEDDLVKGLVKLVRDTIGPVAAFRKVFFVKALPKTRSGKIPRSSLANLVNQKPYKISPTIEDPEVFKDIEKMLRES 626

HSP 2 Score: 70.8626 bits (172), Expect = 3.882e-91
Identity = 34/84 (40.48%), Postives = 55/84 (65.48%), Query Frame = 2 
Query:    2 PVINATEKVSYKQLLTLVENLAGVMVNKCHVQKGDRVLLYVPVTVQGIAAILACTRIGAVYVVTFGGFAPDLIAARIKHTEPKI 253
            PV    + ++Y+++   V  LAGV+V K  V+ GD V++Y+P+  Q +  +LAC RIGA + + FGGFA   +++RI H +PK+
Sbjct:   76 PVTGTKQVITYREVQEQVSRLAGVLV-KHGVKMGDLVVIYMPMVPQAMFTMLACARIGATHSLIFGGFASKELSSRIDHAKPKL 158
[Close]
BLAST of Propionyl-CoA synthetase vs. Ensembl Zebrafish
Match: acss3 (acyl-CoA synthetase short chain family member 3 [Source:ZFIN;Acc:ZDB-GENE-060503-816])

HSP 1 Score: 258.07 bits (658), Expect = 5.928e-83
Identity = 160/472 (33.90%), Postives = 255/472 (54.03%), Query Frame = 3 
Query:  249 KFIITTNFGYEPERTVNYKEIVDEAINKSQFCPDYCIVYNRPNTKCATMIDSRDLDWETEIGKATPYPPVALESGDPIFIIYTSGTLGEPKGLVMTVGGFVVSNKYFMKYVLGLNRRDCWWMFGDYGWISGQTTMTFGPLLIGCSTILYEGKELSSHHFTGIGRIIDQNNVTGFSAAPTYFRFLKNKDSEKD---IKPSKAQLRFVCIGGELSNTKLKEWVSNVFLCPVYDLWGQTELG--IIATGPGLGFKNISSNGLS--PMLSVHPKVVNSATGEQLPNETIGRMLLKIPLCPSAASSMYNDHDRFLKTYFSKXXXXXXXXXXXXIMNDGTLQILARADDVISVSAGRICGGALEEVCLMNEDLANAAFVALEDPINGQVPFAFLEKKKDRNINNEALIIESVKLVKKEIGSFTLFHQGVVVHDLPKTKTGKILRKTLYNMLNGQDWKTPATVENPKVFEEIYSILIEA 1643
            K ++T +FG EP R V Y  +V++A+  S   P   ++YNRP+ +  +      LDWE E+  A P+  V++ +  P++++YTSGT G PK                            WW   D GW+ G + + +GPLL G +T+LYEGK + +       R++ ++       APT  R ++ +D + +   + P   +LR + + GE  + +  EW    F  PV D W QTE G  I A+  GLG       G +  P+   +  V++    +Q+   T+G +++K+PL P AA S++ +H  F + YF+K+ GYYDTMD G +  +G L I+AR+DDVI+V+  R+  GALEE  L++  + + A V LED + G VP A    + D   + + L+   VKLV+  IG    F +   V  LPKT++GKI R +L N++N + +K   T+E+P+VF++I  +L E+
Sbjct:  239 KLLVTASFGIEPGRLVEYIPLVEKALELSTHKPHKVLIYNRPSMEKVSTRPDLTLDWEEEMSTARPHDCVSVSAHHPLYVLYTSGTTGSPK---------------------------VWWAASDLGWVVGHSYICYGPLLHGNTTVLYEGKPVGTPDPGAFFRVMSEHGTACMFTAPTAIRAIRQQDPQAEHGKLYPLN-RLRNLFVAGERCDIETLEWAKKSFGVPVLDHWWQTESGSSITASCVGLGCSLAPPPGTAGKPVPGYNVTVIDDDM-QQVKPRTLGNIVVKLPLPPGAALSLWQNHMLFKELYFTKFTGYYDTMDAGFVDEEGFLYIMARSDDVINVAGHRLSTGALEESVLLHPAVVDCAVVGLEDSLKGHVPLALCVLRNDCKESEDDLVKGLVKLVRDTIGPVAAFRKVFFVKALPKTRSGKIPRSSLANLVNQKPYKISPTIEDPEVFKDIEKMLRES 681

HSP 2 Score: 70.4774 bits (171), Expect = 5.928e-83
Identity = 34/84 (40.48%), Postives = 55/84 (65.48%), Query Frame = 2 
Query:    2 PVINATEKVSYKQLLTLVENLAGVMVNKCHVQKGDRVLLYVPVTVQGIAAILACTRIGAVYVVTFGGFAPDLIAARIKHTEPKI 253
            PV    + ++Y+++   V  LAGV+V K  V+ GD V++Y+P+  Q +  +LAC RIGA + + FGGFA   +++RI H +PK+
Sbjct:  158 PVTGTKQVITYREVQEQVSRLAGVLV-KHGVKMGDLVVIYMPMVPQAMFTMLACARIGATHSLIFGGFASKELSSRIDHAKPKL 240
[Close]
BLAST of Propionyl-CoA synthetase vs. Ensembl Zebrafish
Match: acss1 (acyl-CoA synthetase short chain family member 1 [Source:ZFIN;Acc:ZDB-GENE-050320-139])

HSP 1 Score: 194.512 bits (493), Expect = 6.603e-64
Identity = 136/470 (28.94%), Postives = 227/470 (48.30%), Query Frame = 3 
Query:  249 KFIITTNFGYEPERTVNYKEIVDEAINKSQFCPDYCIVYNRPNTKCATMIDSRDLDWETEI-GKATPYPPVALESGDPIFIIYTSGTLGEPKGLVMTVGGFVVSNKYFMKYVLGLNRRDCWWMFGDYGWISGQTTMTFGPLLIGCSTILYEGKELSSHHFTGIGR---IIDQNNVTGFSAAPTYFRFLKNKDSEKDIKPSKAQLRFVCIGGELSNTKLKEWVSNVF---LCPVYDLWGQTELGIIATGP---GLGFKNISSNGLSPMLSVHPKVVNSATGEQLPNETIGRMLLKIPLCPSAASSMYNDHDRFLKTYFSKXXXXXXXXXXXXIMNDGTLQILARADDVISVSAGRICGGALEEVCLMNEDLANAAFVALEDPINGQVPFAFLEKKKDRNINNEALIIESVKLVKKEIGSFTLFHQGVVVHDLPKTKTGKILRKTLYNML--NGQDWKTPATVENPKVFEEI 1622
            KF+IT N G    R  + K  VD+A+     CP    V+    T  +  +   D+  E  + G+++   P  +ES + +F++YTSG+ G+PKG+V T  G+++      +YV      D +    D GWI+G + + +GPL  G +++L+E    S+  +   GR    + +  +  F  APT  R L   +     K  ++ L+ +   GE  N +  +W  NV     CP+ D W QTE G +   P     G +   +  + P   + P+++         N+  G + +  P  P  A +++ DH RF+  YF  Y G Y T D      DG  QI  R DDVI++S  R+    +E+    + D+   A + +   I G+VPFAF+  K+    N +A++ E   LV  +I  + +    +VV  LPKT++GKI+R+ L  +   +  +    +T+++P V  EI
Sbjct:  218 KFVITCNQGVRGGRVFDLKSTVDKAVKS---CPSVRHVFVAKRTNNSVPMGKLDIPLEEVMAGQSSECAPEPMESEEMLFMLYTSGSTGKPKGIVHTQAGYLLYASLTHQYVFDYTPGDVFGCVADIGWITGHSYVVYGPLCNGATSVLFE----STPVYPNPGRYWETVQRLRINQFYGAPTAIRLLLKYEESWVKKYDRSSLKTLASVGEPINHEAWDWFHNVVGDGRCPLVDTWWQTETGGVCIAPLPAEPGAEIRPAMAMRPFFGIKPELLGEKGQIITGNDVNGALCISQPW-PGMARTIFGDHQRFVDAYFKPYQGQYFTGDGAYRTEDGYYQITGRMDDVINISGHRLGTAEIEDALDEHPDVPETAVIGIPHEIKGEVPFAFVVLKESAAENQQAVVNELRHLVASKIAKYAVPDHFLVVKRLPKTRSGKIMRRILRKVAVQDTSNLGDVSTLDDPSVVSEI 679

HSP 2 Score: 70.8626 bits (172), Expect = 6.603e-64
Identity = 34/79 (43.04%), Postives = 54/79 (68.35%), Query Frame = 2 
Query:   20 EKVSYKQLLTLVENLAGVMVNKCH-VQKGDRVLLYVPVTVQGIAAILACTRIGAVYVVTFGGFAPDLIAARIKHTEPKI 253
            E+++Y++LL +   LA  +  K H VQ+G+RV +Y+PV+   +AA+LAC RIGAV+ V F GF+ + +A RI+  + K 
Sbjct:  143 ERITYRELLEMTCRLANTL--KSHGVQRGERVAIYMPVSPMAVAAMLACARIGAVHTVVFAGFSSEALAGRIQDAQCKF 219
[Close]
BLAST of Propionyl-CoA synthetase vs. Ensembl Zebrafish
Match: acss2 (acyl-CoA synthetase short chain family member 2 [Source:ZFIN;Acc:ZDB-GENE-040718-388])

HSP 1 Score: 186.037 bits (471), Expect = 1.555e-58
Identity = 149/498 (29.92%), Postives = 240/498 (48.19%), Query Frame = 3 
Query:  222 LLELNIQSQK-FIITTNFGYEPERTVNYKEIVDEAINKSQ---FCPDYCIVYNR--------------PNTKCATMID-----SRDLDWETEIGKATPY-PPVALESGDPIFIIYTSGTLGEPKGLVMTVGGFVVSNKYFMKYVLGLNRRDCWWMFGDYGWISGQTTMTFGPLLIGCSTILYEGKELSSH-HFTGIGRIIDQNNVTGFSAAPTYFRFLKNKDSEKDIKPSKAQLRFVCIGGELSNTKLKEWVSNVF---LCPVYDLWGQTELGIIATGPGLGFKNISSNGLS-PMLSVHPKVVNSATGEQLPNETIGRMLLKIPLCPSAASSMYNDHDRFLKTYFSKXXXXXXXXXXXXIMNDGTLQILARADDVISVSAGRICGGALEEVCLMNEDLANAAFVALEDPINGQVPFAFLEKKKDRNINNEALIIESVKLVKKEIGSFTLFHQGVVVHDLPKTKTGKILRKTLYNM-LNGQDWKTPATVENPKVFEEIY 1625
            L E  + SQ   +IT +  Y  ++ +N K I DEA+ K +   F  + CI+                 P  +    +        D+ W + IG A+    PV  +S DP+FI+YTSG+ G+PKG++ TV G+++      K V   +  D +W   D GWI+G + +T+GPL  G +++L+EG  L ++   + +  I+D+ +V+ F  APT  R L    S+   K  +  L+ +   GE  N +  +W  NV     CPV D + QTE G     P      +     + P   V P ++N  +GE+L   + G ++ K P  P    ++Y +H RF  TYF K+ GYY T D      DG   I  R DD+++VS   +    +E   + +E +A AA V    P+ G+  + F+      N  N+ L  E  K V+++IG+            LPKT++GKI+R+ L  +  N +D    +T+ +  V E ++
Sbjct:  182 LCERIMDSQCCLLITADGFYRGDKLINLKLIADEALKKCRDKSFPVEKCIMLKHLTKEDEASSGSLSPPAKRACPDLQVPWNPEVDMCWHSLIGGASEECEPVWCDSEDPLFILYTSGSTGKPKGVLHTVSGYMLYTASTFKMVFDYHSDDVYWCTADIGWITGHSYITYGPLANGATSVLFEG--LPTYPDVSRMWEIVDKYHVSKFYTAPTAIRLLMKYGSDPVHKYKRTSLKILGTVGEPINPEAWQWYYNVVGEKRCPVVDTFWQTETGGHVMTPLPAATPMKPGSATFPFFGVVPAILNE-SGEELEGPSEGYLVFKQPW-PGVMRTVYGNHLRFETTYFKKFPGYYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALVEHEAVAEAAVVGRPHPVKGESLYCFVTLNDGINY-NQKLEAELKKQVREKIGAIATPDYIQNAPGLPKTRSGKIMRRVLRKIACNERDLGDVSTLADSSVIEHLF 674

HSP 2 Score: 61.6178 bits (148), Expect = 1.555e-58
Identity = 30/73 (41.10%), Postives = 50/73 (68.49%), Query Frame = 2 
Query:   26 VSYKQLLTLVENLAGVMVNKCHVQKGDRVLLYVPVTVQGIAAILACTRIGAVYVVTFGGFAPDLIAARIKHTE 244
            V+Y++LL  V   A V+ ++  V+KGDRV +Y+P+ V+ + A+LAC RIGAV+ + F GF+ + +  RI  ++
Sbjct:  119 VTYRELLQQVCKFANVLKSQ-GVKKGDRVSIYMPMVVELVVAMLACARIGAVHSIVFAGFSSESLCERIMDSQ 190
[Close]
BLAST of Propionyl-CoA synthetase vs. Ensembl Zebrafish
Match: acss2 (acyl-CoA synthetase short chain family member 2 [Source:ZFIN;Acc:ZDB-GENE-040718-388])

HSP 1 Score: 180.259 bits (456), Expect = 3.515e-50
Identity = 129/409 (31.54%), Postives = 206/409 (50.37%), Query Frame = 3 
Query:  417 WETEIGKATPY-PPVALESGDPIFIIYTSGTLGEPKGLVMTVGGFVVSNKYFMKYVLGLNRRDCWWMFGDYGWISGQTTMTFGPLLIGCSTILYEGKELSSHHFTGIGRIIDQNNVTGFSAAPTYFRFLKNKDSEKDIKPSKAQLRFVCIGGELSNTKLKEWVSNVF---LCPVYDLWGQTELGIIATGPGLGFKNISSNGLS-PMLSVHPKVVNSATGEQLPNETIGRMLLKIPLCPSAASSMYNDHDRFLKTYFSKXXXXXXXXXXXXIMNDGTLQILARADDVISVSAGRICGGALEEVCLMNEDLANAAFVALEDPINGQVPFAFLEKKKDRNINNEALIIESVKLVKKEIGSFTLFHQGVVVHDLPKTKTGKILRKTLYNM-LNGQDWKTPATVENPKVFEEIY 1625
            W + IG A+    PV  +S DP+FI+YTSG+ G+PKG++ TV G+++      K V   +  D +W   D GWI+G + +T+GPL  G +++L+EG   +    + +  I+D+ +V+ F  APT  R L    S+   K  +  L+ +   GE  N +  +W  NV     CPV D + QTE G     P      +     + P   V P ++N  +GE+L   + G ++ K P  P    ++Y +H RF  TYF K+ GYY T D      DG   I  R DD+++VS   +    +E   + +E +A AA V    P+ G+  + F+      N  N+ L  E  K V+++IG+            LPKT++GKI+R+ L  +  N +D    +T+ +  V E ++
Sbjct:    3 WHSLIGGASEECEPVWCDSEDPLFILYTSGSTGKPKGVLHTVSGYMLYTASTFKMVFDYHSDDVYWCTADIGWITGHSYITYGPLANGATSVLFEGLP-TYPDVSRMWEIVDKYHVSKFYTAPTAIRLLMKYGSDPVHKYKRTSLKILGTVGEPINPEAWQWYYNVVGEKRCPVVDTFWQTETGGHVMTPLPAATPMKPGSATFPFFGVVPAILNE-SGEELEGPSEGYLVFKQPW-PGVMRTVYGNHLRFETTYFKKFPGYYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALVEHEAVAEAAVVGRPHPVKGESLYCFVTLNDGINY-NQKLEAELKKQVREKIGAIATPDYIQNAPGLPKTRSGKIMRRVLRKIACNERDLGDVSTLADSSVIEHLF 407
[Close]
BLAST of Propionyl-CoA synthetase vs. Ensembl Xenopus
Match: ACSS3 (acyl-CoA synthetase short chain family member 3 [Source:NCBI gene;Acc:100489184])

HSP 1 Score: 310.842 bits (795), Expect = 2.758e-101
Identity = 175/464 (37.72%), Postives = 272/464 (58.62%), Query Frame = 3 
Query:  249 KFIITTNFGYEPERTVNYKEIVDEAINKSQFCPDYCIVYNRPNTKCATMIDSRDLDWETEIGKATPYPPVALESGDPIFIIYTSGTLGEPKGLVMTVGGFVVSNKYFMKYVLGLNRRDCWWMFGDYGWISGQTTMTFGPLLIGCSTILYEGKELSSHHFTGIGRIIDQNNVTGFSAAPTYFRFLKNKDSEKDI-KP-SKAQLRFVCIGGELSNTKLKEWVSNVFLCPVYDLWGQTELG--IIATGPGLG--FKNISSNGLSPMLSVHPKVVNSATGEQLPNETIGRMLLKIPLCPSAASSMYNDHDRFLKTYFSKXXXXXXXXXXXXIMNDGTLQILARADDVISVSAGRICGGALEEVCLMNEDLANAAFVALEDPINGQVPFAFLEKKKDRNINNEALIIESVKLVKKEIGSFTLFHQGVVVHDLPKTKTGKILRKTLYNMLNGQDWKTPATVENPKVFEEI 1622
            K IIT +FG EP R V Y  +V+ A+  ++  P+  ++Y+RPN K   +   RDLDW  EI KA P+  V + S  P++++YTSGT G PKG+V +  G+ V   + M  + GLN  D WW   D GW+ G + + +GPLL G +++LYEGK + +   +   R++ ++ V     APT  R ++ +D E ++ KP S  + R + + GE  + +  EW   +F  PV D W QTE G  I AT  GLG   K  +     P+   + K++++   E  PN T+G +++K+PL P A ++++ +   F   YF+K+ GYYDTMD G    DG + +L+R DDVI+V+  R+  GA+EE  L+++ +A+ A V LEDP+ G VP A    +   +   E +  E V LV++ IG    F + V+V  LPKT++GKI R TL  ++NG+ +K  +T+E+P VF+ +
Sbjct:  242 KLIITASFGIEPGRKVEYVPLVERALEIAEHKPEKIVIYSRPNMKEVPLKAGRDLDWTEEISKAHPHDCVPVSSEHPLYVLYTSGTTGLPKGVVRSSAGYTVMLNWTMSSIYGLNPGDVWWAASDLGWVVGHSYICYGPLLHGNTSVLYEGKPVGTPDASAYFRVLSEHGVAAMFTAPTALRAIRQQDPEAELGKPFSLTKFRTLFVAGERCDVETLEWSRQIFKVPVLDHWWQTETGSPITATCIGLGNSLKPPAGQAGKPVPGYNVKILDNDMQEANPN-TLGNIVVKLPLPPGAFATLWKNDAMFQDLYFTKFPGYYDTMDAGYKDEDGYIYVLSRVDDVINVAGHRLSAGAIEESVLLHKAVADCAVVGLEDPLKGHVPLALCVLRNGVDKTTEKIENEIVALVREHIGPVAAFRKVVIVKQLPKTRSGKIPRSTLSALVNGKSYKIGSTIEDPDVFKYV 704

HSP 2 Score: 79.7221 bits (195), Expect = 2.758e-101
Identity = 38/84 (45.24%), Postives = 56/84 (66.67%), Query Frame = 2 
Query:    2 PVINATEKVSYKQLLTLVENLAGVMVNKCHVQKGDRVLLYVPVTVQGIAAILACTRIGAVYVVTFGGFAPDLIAARIKHTEPKI 253
            PV N  + ++YK++   V  LA V+  K  V+KGDRV++Y+P+  Q   A+LAC RIGA++ + FGGFA   +A RI H +PK+
Sbjct:  161 PVTNTKQAITYKEIYEQVSRLASVLC-KYGVKKGDRVVIYMPMIPQATYAMLACARIGAIHSLIFGGFASKELAVRIDHAKPKL 243
[Close]
BLAST of Propionyl-CoA synthetase vs. Ensembl Xenopus
Match: ACSS3 (acyl-CoA synthetase short chain family member 3 [Source:NCBI gene;Acc:100489184])

HSP 1 Score: 299.286 bits (765), Expect = 6.921e-98
Identity = 170/450 (37.78%), Postives = 262/450 (58.22%), Query Frame = 3 
Query:  249 KFIITTNFGYEPERTVNYKEIVDEAINKSQFCPDYCIVYNRPNTKCATMIDSRDLDWETEIGKATPYPPVALESGDPIFIIYTSGTLGEPKGLVMTVGGFVVSNKYFMKYVLGLNRRDCWWMFGDYGWISGQTTMTFGPLLIGCSTILYEGKELSSHHFTGIGRIIDQNNVTGFSAAPTYFRFLKNKDSEKDI-KP-SKAQLRFVCIGGELSNTKLKEWVSNVFLCPVYDLWGQTELG--IIATGPGLG--FKNISSNGLSPMLSVHPKVVNSATGEQLPNETIGRMLLKIPLCPSAASSMYNDHDRFLKTYFSKXXXXXXXXXXXXIMNDGTLQILARADDVISVSAGRICGGALEEVCLMNEDLANAAFVALEDPINGQVPFAFLEKKKDRNINNEALIIESVKLVKKEIGSFTLFHQGVVVHDLPKTKTGKILRKTLYNMLNGQDWK 1580
            K IIT +FG EP R V Y  +V+ A+  ++  P+  ++Y+RPN K   +   RDLDW  EI KA P+  V + S  P++++YTSGT G PKG+V +  G+ V   + M  + GLN  D WW   D GW+ G + + +GPLL G +++LYEGK + +   +   R++ ++ V     APT  R ++ +D E ++ KP S  + R + + GE  + +  EW   +F  PV D W QTE G  I AT  GLG   K  +     P+   + K++++   E  PN T+G +++K+PL P A ++++ +   F   YF+K+ GYYDTMD G    DG + +L+R DDVI+V+  R+  GA+EE  L+++ +A+ A V LEDP+ G VP A    +   +   E +  E V LV++ IG    F + V+V  LPKT++GKI R TL  ++NG+ +K
Sbjct:  242 KLIITASFGIEPGRKVEYVPLVERALEIAEHKPEKIVIYSRPNMKEVPLKAGRDLDWTEEISKAHPHDCVPVSSEHPLYVLYTSGTTGLPKGVVRSSAGYTVMLNWTMSSIYGLNPGDVWWAASDLGWVVGHSYICYGPLLHGNTSVLYEGKPVGTPDASAYFRVLSEHGVAAMFTAPTALRAIRQQDPEAELGKPFSLTKFRTLFVAGERCDVETLEWSRQIFKVPVLDHWWQTETGSPITATCIGLGNSLKPPAGQAGKPVPGYNVKILDNDMQEANPN-TLGNIVVKLPLPPGAFATLWKNDAMFQDLYFTKFPGYYDTMDAGYKDEDGYIYVLSRVDDVINVAGHRLSAGAIEESVLLHKAVADCAVVGLEDPLKGHVPLALCVLRNGVDKTTEKIENEIVALVREHIGPVAAFRKVVIVKQLPKTRSGKIPRSTLSALVNGKSYK 690

HSP 2 Score: 79.7221 bits (195), Expect = 6.921e-98
Identity = 38/84 (45.24%), Postives = 56/84 (66.67%), Query Frame = 2 
Query:    2 PVINATEKVSYKQLLTLVENLAGVMVNKCHVQKGDRVLLYVPVTVQGIAAILACTRIGAVYVVTFGGFAPDLIAARIKHTEPKI 253
            PV N  + ++YK++   V  LA V+  K  V+KGDRV++Y+P+  Q   A+LAC RIGA++ + FGGFA   +A RI H +PK+
Sbjct:  161 PVTNTKQAITYKEIYEQVSRLASVLC-KYGVKKGDRVVIYMPMIPQATYAMLACARIGAIHSLIFGGFASKELAVRIDHAKPKL 243
[Close]
BLAST of Propionyl-CoA synthetase vs. Ensembl Xenopus
Match: ACSS3 (acyl-CoA synthetase short chain family member 3 [Source:NCBI gene;Acc:100489184])

HSP 1 Score: 291.582 bits (745), Expect = 1.653e-95
Identity = 170/449 (37.86%), Postives = 254/449 (56.57%), Query Frame = 3 
Query:  249 KFIITTNFGYEPERTVNYKEIVDEAINKSQFCPDYCIVYNRPNTKCATMIDSRDLDWETEIGKATPYPPVALESGDPIFIIYTSGTLGEPKGLVMTVGGFVVSNKYFMKYVLGLNRRDCWWMFGDYGWISGQTTMTFGPLLIGCSTILYEGKELSSHHFTGIGRIIDQNNVTGFSAAPTYFRFLKNKDSEKDI-KP-SKAQLRFVCIGGELSNTKLKEWVSNVFLCPVYDLWGQTELG--IIATGPGLGFKNISSNGLSPMLSVHPKVVNSATGEQ-LPNETIGRMLLKIPLCPSAASSMYNDHDRFLKTYFSKXXXXXXXXXXXXIMNDGTLQILARADDVISVSAGRICGGALEEVCLMNEDLANAAFVALEDPINGQVPFAFLEKKKDRNINNEALIIESVKLVKKEIGSFTLFHQGVVVHDLPKTKTGKILRKTLYNMLNGQDWK 1580
            K IIT +FG EP R V Y  +V+ A+  ++  P+  ++Y+RPN K   +   RDLDW  EI KA P+  V + S  P++++YTSGT G PKG+V +  G+ V   + M  + GLN  D WW   D GW+ G + + +GPLL G +++LYEGK + +   +   R++ ++ V     APT  R ++ +D E ++ KP S  + R + + GE  + +  EW   +F  PV D W QTE G  I AT  GLG      N L P      K V    G+Q L    +    L++PL P A ++++ +   F   YF+K+ GYYDTMD G    DG + +L+R DDVI+V+  R+  GA+EE  L+++ +A+ A V LEDP+ G VP A    +   +   E +  E V LV++ IG    F + V+V  LPKT++GKI R TL  ++NG+ +K
Sbjct:  242 KLIITASFGIEPGRKVEYVPLVERALEIAEHKPEKIVIYSRPNMKEVPLKAGRDLDWTEEISKAHPHDCVPVSSEHPLYVLYTSGTTGLPKGVVRSSAGYTVMLNWTMSSIYGLNPGDVWWAASDLGWVVGHSYICYGPLLHGNTSVLYEGKPVGTPDASAYFRVLSEHGVAAMFTAPTALRAIRQQDPEAELGKPFSLTKFRTLFVAGERCDVETLEWSRQIFKVPVLDHWWQTETGSPITATCIGLG------NSLKPPAGQAGKPVPGYNGKQNLRKLLLATYPLRLPLPPGAFATLWKNDAMFQDLYFTKFPGYYDTMDAGYKDEDGYIYVLSRVDDVINVAGHRLSAGAIEESVLLHKAVADCAVVGLEDPLKGHVPLALCVLRNGVDKTTEKIENEIVALVREHIGPVAAFRKVVIVKQLPKTRSGKIPRSTLSALVNGKSYK 684

HSP 2 Score: 79.7221 bits (195), Expect = 1.653e-95
Identity = 38/84 (45.24%), Postives = 56/84 (66.67%), Query Frame = 2 
Query:    2 PVINATEKVSYKQLLTLVENLAGVMVNKCHVQKGDRVLLYVPVTVQGIAAILACTRIGAVYVVTFGGFAPDLIAARIKHTEPKI 253
            PV N  + ++YK++   V  LA V+  K  V+KGDRV++Y+P+  Q   A+LAC RIGA++ + FGGFA   +A RI H +PK+
Sbjct:  161 PVTNTKQAITYKEIYEQVSRLASVLC-KYGVKKGDRVVIYMPMIPQATYAMLACARIGAIHSLIFGGFASKELAVRIDHAKPKL 243
[Close]
BLAST of Propionyl-CoA synthetase vs. Ensembl Xenopus
Match: ACSS3 (acyl-CoA synthetase short chain family member 3 [Source:NCBI gene;Acc:100489184])

HSP 1 Score: 296.975 bits (759), Expect = 4.518e-91
Identity = 170/459 (37.04%), Postives = 264/459 (57.52%), Query Frame = 3 
Query:  225 LELNIQSQKFIITTNFGYEPERTVNYKEIVDEAINKSQFCPDYCIVYNRPNTKCATMIDSRDLDWETEIGKATPYPPVALESGDPIFIIYTSGTLGEPKGLVMTVGGFVVSNKYFMKYVLGLNRRDCWWMFGDYGWISGQTTMTFGPLLIGCSTILYEGKELSSHHFTGIGRIIDQNNVTGFSAAPTYFRFLKNKDSEKDI-KP-SKAQLRFVCIGGELSNTKLKEWVSNVFLCPVYDLWGQTELG--IIATGPGLG--FKNISSNGLSPMLSVHPKVVNSATGEQLPNETIGRMLLKIPLCPSAASSMYNDHDRFLKTYFSKXXXXXXXXXXXXIMNDGTLQILARADDVISVSAGRICGGALEEVCLMNEDLANAAFVALEDPINGQVPFAFLEKKKDRNINNEALIIESVKLVKKEIGSFTLFHQGVVVHDLPKTKTGKILRKTLYNMLNGQDWKT 1583
            + ++    K IIT +FG EP R V Y  +V+ A+  ++  P+  ++Y+RPN  C      RDLDW  EI KA P+  V + S  P++++YTSGT G PKG+V +  G+ V   + M  + GLN  D WW   D GW+ G + + +GPLL G +++LYEGK + +   +   R++ ++ V     APT  R ++ +D E ++ KP S  + R + + GE  + +  EW   +F  PV D W QTE G  I AT  GLG   K  +     P+   + K++++   E  PN T+G +++K+PL P A ++++ +   F   YF+K+ GYYDTMD G    DG + +L+R DDVI+V+  R+  GA+EE  L+++ +A+ A V LEDP+ G VP A    +   +   E +  E V LV++ IG    F + V+V  LPKT++GKI R TL  ++NG+ +K 
Sbjct:  230 VRIDHAKPKLIITASFGIEPGRKVEYVPLVERALEIAEHKPEKIVIYSRPNMVCNA---GRDLDWTEEISKAHPHDCVPVSSEHPLYVLYTSGTTGLPKGVVRSSAGYTVMLNWTMSSIYGLNPGDVWWAASDLGWVVGHSYICYGPLLHGNTSVLYEGKPVGTPDASAYFRVLSEHGVAAMFTAPTALRAIRQQDPEAELGKPFSLTKFRTLFVAGERCDVETLEWSRQIFKVPVLDHWWQTETGSPITATCIGLGNSLKPPAGQAGKPVPGYNVKILDNDMQEANPN-TLGNIVVKLPLPPGAFATLWKNDAMFQDLYFTKFPGYYDTMDAGYKDEDGYIYVLSRVDDVINVAGHRLSAGAIEESVLLHKAVADCAVVGLEDPLKGHVPLALCVLRNGVDKTTEKIENEIVALVREHIGPVAAFRKVVIVKQLPKTRSGKIPRSTLSALVNGKSYKA 684

HSP 2 Score: 79.7221 bits (195), Expect = 5.938e-15
Identity = 38/84 (45.24%), Postives = 56/84 (66.67%), Query Frame = 2 
Query:    2 PVINATEKVSYKQLLTLVENLAGVMVNKCHVQKGDRVLLYVPVTVQGIAAILACTRIGAVYVVTFGGFAPDLIAARIKHTEPKI 253
            PV N  + ++YK++   V  LA V+  K  V+KGDRV++Y+P+  Q   A+LAC RIGA++ + FGGFA   +A RI H +PK+
Sbjct:  157 PVTNTKQAITYKEIYEQVSRLASVLC-KYGVKKGDRVVIYMPMIPQATYAMLACARIGAIHSLIFGGFASKELAVRIDHAKPKL 239
[Close]
BLAST of Propionyl-CoA synthetase vs. Ensembl Xenopus
Match: ACSS3 (acyl-CoA synthetase short chain family member 3 [Source:NCBI gene;Acc:100489184])

HSP 1 Score: 269.24 bits (687), Expect = 7.746e-89
Identity = 161/450 (35.78%), Postives = 248/450 (55.11%), Query Frame = 3 
Query:  249 KFIITTNFGYEPERTVNYKEIVDEAINKSQFCPDYCIVYNRPNTKCATMIDSRDLDWETEIGKATPYPPVALESGDPIFIIYTSGTLGEPKGLVMTVGGFVVSNKYFMKYVLGLNRRDCWWMFGDYGWISGQTTMTFGPLLIGCSTILYEGKELSSHHFTGIGRIIDQNNVTGFSAAPTYFRFLKNKDSEKDI-KP-SKAQLRFVCIGGELSNTKLKEWVSNVFLCPVYDLWGQTELG--IIATGPGLG--FKNISSNGLSPMLSVHPKVVNSATGEQLPNETIGRMLLKIPLCPSAASSMYNDHDRFLKTYFSKXXXXXXXXXXXXIMNDGTLQILARADDVISVSAGRICGGALEEVCLMNEDLANAAFVALEDPINGQVPFAFLEKKKDRNINNEALIIESVKLVKKEIGSFTLFHQGVVVHDLPKTKTGKILRKTLYNMLNGQDWK 1580
            K IIT +FG EP R V Y  +V+ A+  ++  P+  ++Y+RPN K   +   RDLDW  EI KA P+  V + S  P++++YTSGT G PK                            WW   D GW+ G + + +GPLL G +++LYEGK + +   +   R++ ++ V     APT  R ++ +D E ++ KP S  + R + + GE  + +  EW   +F  PV D W QTE G  I AT  GLG   K  +     P+   + K++++   E  PN T+G +++K+PL P A ++++ +   F   YF+K+ GYYDTMD G    DG + +L+R DDVI+V+  R+  GA+EE  L+++ +A+ A V LEDP+ G VP A    +   +   E +  E V LV++ IG    F + V+V  LPKT++GKI R TL  ++NG+ +K
Sbjct:  242 KLIITASFGIEPGRKVEYVPLVERALEIAEHKPEKIVIYSRPNMKEVPLKAGRDLDWTEEISKAHPHDCVPVSSEHPLYVLYTSGTTGLPK---------------------------VWWAASDLGWVVGHSYICYGPLLHGNTSVLYEGKPVGTPDASAYFRVLSEHGVAAMFTAPTALRAIRQQDPEAELGKPFSLTKFRTLFVAGERCDVETLEWSRQIFKVPVLDHWWQTETGSPITATCIGLGNSLKPPAGQAGKPVPGYNVKILDNDMQEANPN-TLGNIVVKLPLPPGAFATLWKNDAMFQDLYFTKFPGYYDTMDAGYKDEDGYIYVLSRVDDVINVAGHRLSAGAIEESVLLHKAVADCAVVGLEDPLKGHVPLALCVLRNGVDKTTEKIENEIVALVREHIGPVAAFRKVVIVKQLPKTRSGKIPRSTLSALVNGKSYK 663

HSP 2 Score: 79.7221 bits (195), Expect = 7.746e-89
Identity = 38/84 (45.24%), Postives = 56/84 (66.67%), Query Frame = 2 
Query:    2 PVINATEKVSYKQLLTLVENLAGVMVNKCHVQKGDRVLLYVPVTVQGIAAILACTRIGAVYVVTFGGFAPDLIAARIKHTEPKI 253
            PV N  + ++YK++   V  LA V+  K  V+KGDRV++Y+P+  Q   A+LAC RIGA++ + FGGFA   +A RI H +PK+
Sbjct:  161 PVTNTKQAITYKEIYEQVSRLASVLC-KYGVKKGDRVVIYMPMIPQATYAMLACARIGAIHSLIFGGFASKELAVRIDHAKPKL 243
[Close]
BLAST of Propionyl-CoA synthetase vs. Ensembl Mouse
Match: Acss3 (acyl-CoA synthetase short-chain family member 3 [Source:MGI Symbol;Acc:MGI:2685720])

HSP 1 Score: 304.679 bits (779), Expect = 1.044e-99
Identity = 174/473 (36.79%), Postives = 265/473 (56.03%), Query Frame = 3 
Query:  249 KFIITTNFGYEPERTVNYKEIVDEAINKSQFCPDYCIVYNRPNTKCATMIDSRDLDWETEIGKATPYPPVALESGDPIFIIYTSGTLGEPKGLVMTVGGFVVSNKYFMKYVLGLNRRDCWWMFGDYGWISGQTTMTFGPLLIGCSTILYEGKELSSHHFTGIGRIIDQNNVTGFSAAPTYFRFLKNKDSEKDIKPSKAQLRF--VCIGGELSNTKLKEWVSNVFLCPVYDLWGQTELG--IIATGPGLGFKNISSNGLS----PMLSVHPKVVNSATGEQLPNETIGRMLLKIPLCPSAASSMYNDHDRFLKTYFSKXXXXXXXXXXXXIMNDGTLQILARADDVISVSAGRICGGALEEVCLMNEDLANAAFVALEDPINGQVPFAFLEKKKDRNINNEALIIESVKLVKKEIGSFTLFHQGVVVHDLPKTKTGKILRKTLYNMLNGQDWKTPATVENPKVFEEIYSILIEA 1643
            K ++T +FG EP R V Y  +++EA+   Q  PD  ++Y+RPN +   ++  RDLDWE E+ KA  +  V + S  P++I+YTSGT G PKG+V   GG+ V   + M  + GL   + WW   D GW+ G + + +GPLL G +T+LYEGK + +       R++ ++ V     APT  R ++ +D    +    +  RF  + + GE  + +  EW   VF  PV D W QTE G  I A+  GLG       G +    P  +V   ++     ++L   ++G +++K+PL P A S ++ + + F   YF K+ GYYDTMD G +  +G L +++R DDVI+V+  RI  GA+EE  L +  +A+ A V  EDP+ G VP A    KKD N + E ++ E VK V++ IG    F   V V  LPKT++GKI R TL  ++NG+ +K   T+E+P +F  I  +L +A
Sbjct:  212 KVVVTASFGIEPGRKVEYIPLLEEALRIGQHRPDRVLIYSRPNMEKVPLMSGRDLDWEEEMAKAQSHDCVPVLSEHPLYILYTSGTTGLPKGVVRPTGGYAVMLNWTMSSIYGLKPGEVWWAASDLGWVVGHSYICYGPLLHGNTTVLYEGKPVGTPDAGAYFRVLAEHGVAALFTAPTAIRAIRQQDPGAALGKQYSLTRFKTLFVAGERCDVETLEWSKKVFRVPVLDHWWQTETGSPITASCIGLGNSKTPPPGQAGKCVPGYNV---MILDDNMQKLKARSLGNIVVKLPLPPGAFSGLWKNQEAFKHLYFEKFPGYYDTMDAGYMDEEGYLYVMSRVDDVINVAGHRISAGAIEESVLSHGTVADCAVVGKEDPLKGHVPLALCVLKKDVNASEEQVLEEIVKHVRQSIGPVAAFRNAVFVKQLPKTRSGKIPRSTLSALVNGKPYKVTPTIEDPSIFGHIEEVLKQA 681

HSP 2 Score: 80.1073 bits (196), Expect = 1.044e-99
Identity = 39/84 (46.43%), Postives = 57/84 (67.86%), Query Frame = 2 
Query:    2 PVINATEKVSYKQLLTLVENLAGVMVNKCHVQKGDRVLLYVPVTVQGIAAILACTRIGAVYVVTFGGFAPDLIAARIKHTEPKI 253
            PV +    +SYK++L  V  LAGV+V K  V+KGD V++Y+P+  Q I  +LAC RIGA++ + FGGFA   ++ RI H +PK+
Sbjct:  131 PVTDTKATISYKEVLEQVSKLAGVLV-KQGVKKGDTVVIYMPMIPQAIYTMLACARIGAIHSLIFGGFASKELSTRIDHAKPKV 213
[Close]
BLAST of Propionyl-CoA synthetase vs. Ensembl Mouse
Match: Acss3 (acyl-CoA synthetase short-chain family member 3 [Source:MGI Symbol;Acc:MGI:2685720])

HSP 1 Score: 180.644 bits (457), Expect = 1.778e-62
Identity = 90/250 (36.00%), Postives = 136/250 (54.40%), Query Frame = 3 
Query:  249 KFIITTNFGYEPERTVNYKEIVDEAINKSQFCPDYCIVYNRPNTKCATMIDSRDLDWETEIGKATPYPPVALESGDPIFIIYTSGTLGEPKGLVMTVGGFVVSNKYFMKYVLGLNRRDCWWMFGDYGWISGQTTMTFGPLLIGCSTILYEGKELSSHHFTGIGRIIDQNNVTGFSAAPTYFRFLKNKDSEKDIKPSKAQLRF--VCIGGELSNTKLKEWVSNVFLCPVYDLWGQTELGIIATGPGLGFKN 992
            K ++T +FG EP R V Y  +++EA+   Q  PD  ++Y+RPN +   ++  RDLDWE E+ KA  +  V + S  P++I+YTSGT G PKG+V   GG+ V   + M  + GL   + WW   D GW+ G + + +GPLL G +T+LYEGK + +       R++ ++ V     APT  R ++ +D    +    +  RF  + + GE  + +  EW   VF  PV D W QTE G   T   +G  N
Sbjct:  212 KVVVTASFGIEPGRKVEYIPLLEEALRIGQHRPDRVLIYSRPNMEKVPLMSGRDLDWEEEMAKAQSHDCVPVLSEHPLYILYTSGTTGLPKGVVRPTGGYAVMLNWTMSSIYGLKPGEVWWAASDLGWVVGHSYICYGPLLHGNTTVLYEGKPVGTPDAGAYFRVLAEHGVAALFTAPTAIRAIRQQDPGAALGKQYSLTRFKTLFVAGERCDVETLEWSKKVFRVPVLDHWWQTETGSPITASCIGLGN 461

HSP 2 Score: 79.7221 bits (195), Expect = 1.778e-62
Identity = 39/84 (46.43%), Postives = 57/84 (67.86%), Query Frame = 2 
Query:    2 PVINATEKVSYKQLLTLVENLAGVMVNKCHVQKGDRVLLYVPVTVQGIAAILACTRIGAVYVVTFGGFAPDLIAARIKHTEPKI 253
            PV +    +SYK++L  V  LAGV+V K  V+KGD V++Y+P+  Q I  +LAC RIGA++ + FGGFA   ++ RI H +PK+
Sbjct:  131 PVTDTKATISYKEVLEQVSKLAGVLV-KQGVKKGDTVVIYMPMIPQAIYTMLACARIGAIHSLIFGGFASKELSTRIDHAKPKV 213
[Close]
BLAST of Propionyl-CoA synthetase vs. Ensembl Mouse
Match: Acss1 (acyl-CoA synthetase short-chain family member 1 [Source:MGI Symbol;Acc:MGI:1915988])

HSP 1 Score: 196.438 bits (498), Expect = 3.442e-62
Identity = 152/482 (31.54%), Postives = 229/482 (47.51%), Query Frame = 3 
Query:  228 ELNIQSQKFIITTNFGYEPERTVNYKEIVDEAINKSQFCP--DYCIVYNRPNTKCATMIDSRDLDWETEIGKATPY-PPVALESGDPIFIIYTSGTLGEPKGLVMTVGGFVVSNKYFMKYVLGLNRRDCWWMFGDYGWISGQTTMTFGPLLIGCSTILYEGKELSSHHFTGIGR---IIDQNNVTGFSAAPTYFRFLKNKDSEKDIKPSKAQLRFVCIGGELSNTKLKEWVSNVF---LCPVYDLWGQTELGIIATGPGL---GFKNISSNGLSPMLSVHPKVVNSATGEQLPNETIGRMLLKIPLCPSAASSMYNDHDRFLKTYFSKXXXXXXXXXXXXIMNDGTLQILARADDVISVSAGRICGGALEEVCLMNEDLANAAFVALEDPINGQVPFAFLEKKKDRNINNEALIIESVKL-VKKEIGSFTLFHQGVVVHDLPKTKTGKILRKTLYNML--NGQDWKTPATVENPKVFEEIYS 1628
             +N    K +IT N G    R V  K+IVDEA+     CP   + +V +R +TK    + S D+  E E+ K  P   P ++ S D +F++YTSG+ G PKGLV T  G+++      K V      D +    D GWI+G + + +GPL  G +T+L+E    S+  +   GR    + +  +  F  APT  R L         K  ++ LR +   GE  N +  EW+  V     C + D W QTE G I   P     G + +    + P   + P V+    G  L    +   L      P  A ++Y DH RF+  YF  Y GYY T D       G  QI  R DDVI++S  R+    +E+    +  +   A +     I G+  FAF+  K   NI++E +++  +KL V  +I  + +  Q +VV  LPKT++GK++R+ L  ++   GQD     T+E+P V  EI S
Sbjct:  200 RINDAKCKAVITFNQGLRGGRVVELKKIVDEAVKS---CPTVQHVLVAHRTDTKVP--MGSLDIPLEQEMAKEAPVCTPESMSSEDMLFMLYTSGSTGTPKGLVHTQAGYLLYAAMTHKLVFDYQPGDVFGCVADIGWITGHSYVVYGPLCNGATTVLFE----STPVYPDAGRYWETVQRLKINQFYGAPTAVRLLLKYGDAWVKKYDRSSLRTLGSVGEPINHEAWEWLHKVVGDGRCTLVDTWWQTETGGICIAPRPSEDGAEILPGMAMRPFFGIVP-VLMDEKGNVLEGGDVSGALCISQAWPGMARTIYGDHQRFVDAYFRAYPGYYFTGDGAHRTEGGYYQITGRMDDVINISGHRLGTAEIEDAMADHPAVPETAVIGYPHDIKGEAAFAFIVLKD--NISDENMVVNELKLSVATKIAKYAVPDQILVVKRLPKTRSGKVMRRLLRKIITSRGQDLGDTTTLEDPSVITEILS 669

HSP 2 Score: 63.1586 bits (152), Expect = 3.442e-62
Identity = 30/76 (39.47%), Postives = 49/76 (64.47%), Query Frame = 2 
Query:   23 KVSYKQLLTLVENLAGVMVNKCHVQKGDRVLLYVPVTVQGIAAILACTRIGAVYVVTFGGFAPDLIAARIKHTEPK 250
            +++Y++LL     LA  +  +  V +GDRV +Y+PV+   +AA+LAC RIGA++ V F GF+ + +A RI   + K
Sbjct:  133 RITYRELLETTCRLANTL-KRHGVHRGDRVAIYMPVSPLAVAAMLACARIGAIHTVVFAGFSAESLAGRINDAKCK 207
[Close]
BLAST of Propionyl-CoA synthetase vs. Ensembl Mouse
Match: Acss2 (acyl-CoA synthetase short-chain family member 2 [Source:MGI Symbol;Acc:MGI:1890410])

HSP 1 Score: 180.259 bits (456), Expect = 1.531e-56
Identity = 150/490 (30.61%), Postives = 230/490 (46.94%), Query Frame = 3 
Query:  252 FIITTNFGYEPERTVNYKEIVDEAINKSQ---FCPDYCIVYNRPNTKCATMIDSR---------------------DLDWETEIGKA-TPYPPVALESGDPIFIIYTSGTLGEPKGLVMTVGGFVVSNKYFMKYVLGLNRRDCWWMFGDYGWISGQTTMTFGPLLIGCSTILYEGKELSSHHFTG-IGRIIDQNNVTGFSAAPTYFRFLKNKDSEKDIKPSKAQLRFVCIGGELSNTKLKEWVSNVF---LCPVYDLWGQTELGIIATGPGLGFKNISSNGLS-PMLSVHPKVVNSATGEQLPNETIGRMLLKIPLCPSAASSMYNDHDRFLKTYFSKXXXXXXXXXXXXIMNDGTLQILARADDVISVSAGRICGGALEEVCLMNEDLANAAFVALEDPINGQVPFAFLEKKKDRNINNEALIIESVKLVKKEIGSFTLFHQGVVVHDLPKTKTGKILRKTLYNML-NGQDWKTPATVENPKVFEEIYS 1628
             +ITT+  Y  E+ VN KE+ DE++ K +   F    CIV          M DS                      DL W   + +A     P   ++ DP+FI+YTSG+ G+PKG+V T+GG+++      KYV   +  D +W   D GWI+G + +T+GPL  G +++L+EG  + ++   G +  I+D+  VT F  APT  R L     +   K S+A L+ +   GE  N +   W   V     CP+ D + QTE G     P  G   +     S P   V P ++N +  E    E  G ++ K P  P    ++Y +H RF  TYF K+ GYY T D      DG   I  R DD+++VS   +    +E   + +E +A AA V    P+ G+  + F+    D +  +  L  E  K ++++IG             LPKT++GKI+R+ L  +  N  D    +TV +P V   ++S
Sbjct:  210 LLITTDAFYRGEKLVNLKELADESLEKCREKGFPVRCCIVVKHLGRAELGMNDSPSQSPPVKRPCPDVQICWNEGVDLWWHELMQQAGDECEPEWCDAEDPLFILYTSGSTGKPKGVVHTIGGYMLYVATTFKYVFDFHPEDVFWCTADIGWITGHSYVTYGPLANGATSVLFEG--IPTYPDEGRLWSIVDKYKVTKFYTAPTAIRMLMKFGDDPVTKHSRASLQVLGTVGEPINPEAWLWYHRVVGSQRCPIVDTFWQTETGGHMLTPLPGATPMKPGSASFPFFGVAPAILNESGEELE-GEAEGYLVFKQPW-PGIMRTVYGNHTRFETTYFKKFPGYYVTGDGCRRDQDGYYWITGRIDDMLNVSGHLLSTAEVESALVEHEAVAEAAVVGHPHPVKGECLYCFV-TLCDGHTFSPTLTEELKKQIREKIGPIATPDYIQNAPGLPKTRSGKIMRRVLRKIAQNDHDLGDTSTVADPSVINHLFS 694

HSP 2 Score: 60.4622 bits (145), Expect = 1.531e-56
Identity = 28/72 (38.89%), Postives = 49/72 (68.06%), Query Frame = 2 
Query:   17 TEKVSYKQLLTLVENLAGVMVNKCHVQKGDRVLLYVPVTVQGIAAILACTRIGAVYVVTFGGFAPDLIAARI 232
            T K++Y++LL  V   + V+  K  +QKGDRV +Y+P+ ++ + A+LAC R+GA++ + F GF+ + +  RI
Sbjct:  133 TTKITYRELLVQVCQFSNVL-RKQGIQKGDRVAIYMPMILELVVAMLACARLGALHSIVFAGFSAESLCERI 203
[Close]
BLAST of Propionyl-CoA synthetase vs. Ensembl Mouse
Match: Acss2 (acyl-CoA synthetase short-chain family member 2 [Source:MGI Symbol;Acc:MGI:1890410])

HSP 1 Score: 176.792 bits (447), Expect = 1.823e-55
Identity = 150/503 (29.82%), Postives = 232/503 (46.12%), Query Frame = 3 
Query:  252 FIITTNFGYEPERTVNYKEIVDEAINKSQ---FCPDYCIVYN------------------------------RPNTKCA----TMIDSRDLDWETEIGKA-TPYPPVALESGDPIFIIYTSGTLGEPKGLVMTVGGFVVSNKYFMKYVLGLNRRDCWWMFGDYGWISGQTTMTFGPLLIGCSTILYEGKELSSHHFTG-IGRIIDQNNVTGFSAAPTYFRFLKNKDSEKDIKPSKAQLRFVCIGGELSNTKLKEWVSNVF---LCPVYDLWGQTELGIIATGPGLGFKNISSNGLS-PMLSVHPKVVNSATGEQLPNETIGRMLLKIPLCPSAASSMYNDHDRFLKTYFSKXXXXXXXXXXXXIMNDGTLQILARADDVISVSAGRICGGALEEVCLMNEDLANAAFVALEDPINGQVPFAFLEKKKDRNINNEALIIESVKLVKKEIGSFTLFHQGVVVHDLPKTKTGKILRKTLYNML-NGQDWKTPATVENPKVFEEIYS 1628
             +ITT+  Y  E+ VN KE+ DE++ K +   F    CIV                                R  +KC        +  DL W   + +A     P   ++ DP+FI+YTSG+ G+PKG+V T+GG+++      KYV   +  D +W   D GWI+G + +T+GPL  G +++L+EG  + ++   G +  I+D+  VT F  APT  R L     +   K S+A L+ +   GE  N +   W   V     CP+ D + QTE G     P  G   +     S P   V P ++N +  E    E  G ++ K P  P    ++Y +H RF  TYF K+ GYY T D      DG   I  R DD+++VS   +    +E   + +E +A AA V    P+ G+  + F+    D +  +  L  E  K ++++IG             LPKT++GKI+R+ L  +  N  D    +TV +P V   ++S
Sbjct:  210 LLITTDAFYRGEKLVNLKELADESLEKCREKGFPVRCCIVVKHLGRAELGMNDSPSQSPPVKRPCPDVQGKLREKSKCIRPQICWNEGVDLWWHELMQQAGDECEPEWCDAEDPLFILYTSGSTGKPKGVVHTIGGYMLYVATTFKYVFDFHPEDVFWCTADIGWITGHSYVTYGPLANGATSVLFEG--IPTYPDEGRLWSIVDKYKVTKFYTAPTAIRMLMKFGDDPVTKHSRASLQVLGTVGEPINPEAWLWYHRVVGSQRCPIVDTFWQTETGGHMLTPLPGATPMKPGSASFPFFGVAPAILNESGEELE-GEAEGYLVFKQPW-PGIMRTVYGNHTRFETTYFKKFPGYYVTGDGCRRDQDGYYWITGRIDDMLNVSGHLLSTAEVESALVEHEAVAEAAVVGHPHPVKGECLYCFV-TLCDGHTFSPTLTEELKKQIREKIGPIATPDYIQNAPGLPKTRSGKIMRRVLRKIAQNDHDLGDTSTVADPSVINHLFS 707

HSP 2 Score: 60.4622 bits (145), Expect = 1.823e-55
Identity = 28/72 (38.89%), Postives = 49/72 (68.06%), Query Frame = 2 
Query:   17 TEKVSYKQLLTLVENLAGVMVNKCHVQKGDRVLLYVPVTVQGIAAILACTRIGAVYVVTFGGFAPDLIAARI 232
            T K++Y++LL  V   + V+  K  +QKGDRV +Y+P+ ++ + A+LAC R+GA++ + F GF+ + +  RI
Sbjct:  133 TTKITYRELLVQVCQFSNVL-RKQGIQKGDRVAIYMPMILELVVAMLACARLGALHSIVFAGFSAESLCERI 203
[Close]
BLAST of Propionyl-CoA synthetase vs. UniProt/SwissProt
Match: sp|A0A0G2K047|ACSS3_RAT (Acyl-CoA synthetase short-chain family member 3, mitochondrial OS=Rattus norvegicus OX=10116 GN=Acss3 PE=1 SV=1)

HSP 1 Score: 305.064 bits (780), Expect = 2.254e-99
Identity = 174/473 (36.79%), Postives = 263/473 (55.60%), Query Frame = 3 
Query:  249 KFIITTNFGYEPERTVNYKEIVDEAINKSQFCPDYCIVYNRPNTKCATMIDSRDLDWETEIGKATPYPPVALESGDPIFIIYTSGTLGEPKGLVMTVGGFVVSNKYFMKYVLGLNRRDCWWMFGDYGWISGQTTMTFGPLLIGCSTILYEGKELSSHHFTGIGRIIDQNNVTGFSAAPTYFRFLKNKDSEKDIKPSKAQLRF--VCIGGELSNTKLKEWVSNVFLCPVYDLWGQTELG--IIATGPGLGFKNISSNGLS----PMLSVHPKVVNSATGEQLPNETIGRMLLKIPLCPSAASSMYNDHDRFLKTYFSKXXXXXXXXXXXXIMNDGTLQILARADDVISVSAGRICGGALEEVCLMNEDLANAAFVALEDPINGQVPFAFLEKKKDRNINNEALIIESVKLVKKEIGSFTLFHQGVVVHDLPKTKTGKILRKTLYNMLNGQDWKTPATVENPKVFEEIYSILIEA 1643
            K ++T +FG EP R V Y  +++EA+   Q  PD  ++YNRPN +   ++  RDLDWE E+ KA  +  V + S  P++I+YTSGT G PKG+V   GG+ V   + M  + GL   + WW   D GW+ G + + +GPLL G +T+LYEGK + +       R++ ++ V     APT  R ++ +D    +    +  RF  + + GE  + +  EW   VF  PV D W QTE G  I A+  GLG       G +    P  +V   ++     ++L   ++G +++K+PL P A S ++ + + F   YF K+ GYYDTMD G +  +G L +++R DDVI+V+  RI  GA+EE  L +  + + A V  EDP+ G VP A    KKD N   E ++ E VK V++ IG    F   V V  LPKT++GKI R TL  ++NG+ +K   T+E+P +F  I  +L +A
Sbjct:  213 KVVVTASFGIEPGRKVEYMPLLEEALRIGQHKPDRLLIYNRPNMEKVPLMSGRDLDWEEEMAKAQSHDCVPVLSEHPLYILYTSGTTGLPKGVVRPTGGYAVMLNWTMSSIYGLKPGEVWWAASDLGWVVGHSYICYGPLLHGNTTVLYEGKPVGTPDAGAYFRVLAEHGVAALFTAPTAIRAIRQQDPGAALGKQYSLTRFKTLFVAGERCDVETLEWSKKVFRVPVLDHWWQTETGSPITASCIGLGNSKTPPPGQAGKCVPGYNV---MILDDNMQKLKARSLGNIVVKLPLPPGAFSGLWKNQEAFKHLYFEKFPGYYDTMDAGYMDEEGYLYVMSRVDDVINVAGHRISAGAIEESVLSHGTVTDCAVVGKEDPLKGHVPLALCVLKKDVNATEEQVLEEIVKHVRQSIGPVAAFRNAVFVKQLPKTRSGKIPRSTLSALVNGKPYKVTPTIEDPSIFGHIEEVLKQA 682

HSP 2 Score: 81.2629 bits (199), Expect = 2.254e-99
Identity = 40/84 (47.62%), Postives = 58/84 (69.05%), Query Frame = 2 
Query:    2 PVINATEKVSYKQLLTLVENLAGVMVNKCHVQKGDRVLLYVPVTVQGIAAILACTRIGAVYVVTFGGFAPDLIAARIKHTEPKI 253
            PV +    +SYK++L  V  LAGV+V K  V+KGD V++Y+P+  Q I A+LAC RIGA++ + FGGFA   ++ RI H +PK+
Sbjct:  132 PVTDTKATISYKEVLEQVSKLAGVLV-KQGVKKGDTVVIYMPMIPQAIYAMLACARIGAIHSLIFGGFASKELSTRIDHVKPKV 214
[Close]
BLAST of Propionyl-CoA synthetase vs. UniProt/SwissProt
Match: sp|Q14DH7|ACSS3_MOUSE (Acyl-CoA synthetase short-chain family member 3, mitochondrial OS=Mus musculus OX=10090 GN=Acss3 PE=1 SV=2)

HSP 1 Score: 304.679 bits (779), Expect = 6.316e-99
Identity = 174/473 (36.79%), Postives = 265/473 (56.03%), Query Frame = 3 
Query:  249 KFIITTNFGYEPERTVNYKEIVDEAINKSQFCPDYCIVYNRPNTKCATMIDSRDLDWETEIGKATPYPPVALESGDPIFIIYTSGTLGEPKGLVMTVGGFVVSNKYFMKYVLGLNRRDCWWMFGDYGWISGQTTMTFGPLLIGCSTILYEGKELSSHHFTGIGRIIDQNNVTGFSAAPTYFRFLKNKDSEKDIKPSKAQLRF--VCIGGELSNTKLKEWVSNVFLCPVYDLWGQTELG--IIATGPGLGFKNISSNGLS----PMLSVHPKVVNSATGEQLPNETIGRMLLKIPLCPSAASSMYNDHDRFLKTYFSKXXXXXXXXXXXXIMNDGTLQILARADDVISVSAGRICGGALEEVCLMNEDLANAAFVALEDPINGQVPFAFLEKKKDRNINNEALIIESVKLVKKEIGSFTLFHQGVVVHDLPKTKTGKILRKTLYNMLNGQDWKTPATVENPKVFEEIYSILIEA 1643
            K ++T +FG EP R V Y  +++EA+   Q  PD  ++Y+RPN +   ++  RDLDWE E+ KA  +  V + S  P++I+YTSGT G PKG+V   GG+ V   + M  + GL   + WW   D GW+ G + + +GPLL G +T+LYEGK + +       R++ ++ V     APT  R ++ +D    +    +  RF  + + GE  + +  EW   VF  PV D W QTE G  I A+  GLG       G +    P  +V   ++     ++L   ++G +++K+PL P A S ++ + + F   YF K+ GYYDTMD G +  +G L +++R DDVI+V+  RI  GA+EE  L +  +A+ A V  EDP+ G VP A    KKD N + E ++ E VK V++ IG    F   V V  LPKT++GKI R TL  ++NG+ +K   T+E+P +F  I  +L +A
Sbjct:  212 KVVVTASFGIEPGRKVEYIPLLEEALRIGQHRPDRVLIYSRPNMEKVPLMSGRDLDWEEEMAKAQSHDCVPVLSEHPLYILYTSGTTGLPKGVVRPTGGYAVMLNWTMSSIYGLKPGEVWWAASDLGWVVGHSYICYGPLLHGNTTVLYEGKPVGTPDAGAYFRVLAEHGVAALFTAPTAIRAIRQQDPGAALGKQYSLTRFKTLFVAGERCDVETLEWSKKVFRVPVLDHWWQTETGSPITASCIGLGNSKTPPPGQAGKCVPGYNV---MILDDNMQKLKARSLGNIVVKLPLPPGAFSGLWKNQEAFKHLYFEKFPGYYDTMDAGYMDEEGYLYVMSRVDDVINVAGHRISAGAIEESVLSHGTVADCAVVGKEDPLKGHVPLALCVLKKDVNASEEQVLEEIVKHVRQSIGPVAAFRNAVFVKQLPKTRSGKIPRSTLSALVNGKPYKVTPTIEDPSIFGHIEEVLKQA 681

HSP 2 Score: 80.1073 bits (196), Expect = 6.316e-99
Identity = 39/84 (46.43%), Postives = 57/84 (67.86%), Query Frame = 2 
Query:    2 PVINATEKVSYKQLLTLVENLAGVMVNKCHVQKGDRVLLYVPVTVQGIAAILACTRIGAVYVVTFGGFAPDLIAARIKHTEPKI 253
            PV +    +SYK++L  V  LAGV+V K  V+KGD V++Y+P+  Q I  +LAC RIGA++ + FGGFA   ++ RI H +PK+
Sbjct:  131 PVTDTKATISYKEVLEQVSKLAGVLV-KQGVKKGDTVVIYMPMIPQAIYTMLACARIGAIHSLIFGGFASKELSTRIDHAKPKV 213
[Close]
BLAST of Propionyl-CoA synthetase vs. UniProt/SwissProt
Match: sp|Q9H6R3|ACSS3_HUMAN (Acyl-CoA synthetase short-chain family member 3, mitochondrial OS=Homo sapiens OX=9606 GN=ACSS3 PE=1 SV=1)

HSP 1 Score: 302.753 bits (774), Expect = 7.513e-98
Identity = 172/473 (36.36%), Postives = 263/473 (55.60%), Query Frame = 3 
Query:  249 KFIITTNFGYEPERTVNYKEIVDEAINKSQFCPDYCIVYNRPNTKCATMIDSRDLDWETEIGKATPYPPVALESGDPIFIIYTSGTLGEPKGLVMTVGGFVVSNKYFMKYVLGLNRRDCWWMFGDYGWISGQTTMTFGPLLIGCSTILYEGKELSSHHFTGIGRIIDQNNVTGFSAAPTYFRFLKNKDSEKDIKPSKAQLRF--VCIGGELSNTKLKEWVSNVFLCPVYDLWGQTELG--IIATGPGLGFKNISSNGLS----PMLSVHPKVVNSATGEQLPNETIGRMLLKIPLCPSAASSMYNDHDRFLKTYFSKXXXXXXXXXXXXIMNDGTLQILARADDVISVSAGRICGGALEEVCLMNEDLANAAFVALEDPINGQVPFAFLEKKKDRNINNEALIIESVKLVKKEIGSFTLFHQGVVVHDLPKTKTGKILRKTLYNMLNGQDWKTPATVENPKVFEEIYSILIEA 1643
            K ++T +FG EP R V Y  +V+EA+   Q  PD  ++YNRPN +   +   RDLDW+ E+ KA  +  V + S  P++I+YTSGT G PKG++   GG+ V   + M  + GL   + WW   D GW+ G + + +GPLL G +T+LYEGK + +       R++ ++ V     APT  R ++ +D    +    +  RF  + + GE  + +  EW  NVF  PV D W QTE G  I A+  GLG       G +    P  +V   ++     ++L    +G +++K+PL P A S ++ + + F   YF K+ GYYDTMD G +  +G L +++R DDVI+V+  RI  GA+EE  L +  +A+ A V  EDP+ G VP A    +KD N   E ++ E VK V++ IG    F   V V  LPKT++GKI R  L  ++NG+ +K  +T+E+P +F  +  +L +A
Sbjct:  217 KVVVTASFGIEPGRRVEYVPLVEEALKIGQHKPDKILIYNRPNMEAVPLAPGRDLDWDEEMAKAQSHDCVPVLSEHPLYILYTSGTTGLPKGVIRPTGGYAVMLHWSMSSIYGLQPGEVWWAASDLGWVVGHSYICYGPLLHGNTTVLYEGKPVGTPDAGAYFRVLAEHGVAALFTAPTAIRAIRQQDPGAALGKQYSLTRFKTLFVAGERCDVETLEWSKNVFRVPVLDHWWQTETGSPITASCVGLGNSKTPPPGQAGKSVPGYNV---MILDDNMQKLKARCLGNIVVKLPLPPGAFSGLWKNQEAFKHLYFEKFPGYYDTMDAGYMDEEGYLYVMSRVDDVINVAGHRISAGAIEESILSHGTVADCAVVGKEDPLKGHVPLALCVLRKDINATEEQVLEEIVKHVRQNIGPVAAFRNAVFVKQLPKTRSGKIPRSALSAIVNGKPYKITSTIEDPSIFGHVEEMLKQA 686

HSP 2 Score: 78.5666 bits (192), Expect = 7.513e-98
Identity = 37/84 (44.05%), Postives = 57/84 (67.86%), Query Frame = 2 
Query:    2 PVINATEKVSYKQLLTLVENLAGVMVNKCHVQKGDRVLLYVPVTVQGIAAILACTRIGAVYVVTFGGFAPDLIAARIKHTEPKI 253
            PV N     +YK++L  V  LAGV+V K  ++KGD V++Y+P+  Q +  +LAC RIGA++ + FGGFA   +++RI H +PK+
Sbjct:  136 PVTNTKATFTYKEVLEQVSKLAGVLV-KHGIKKGDTVVIYMPMIPQAMYTMLACARIGAIHSLIFGGFASKELSSRIDHVKPKV 218
[Close]
BLAST of Propionyl-CoA synthetase vs. UniProt/SwissProt
Match: sp|Q5REB8|ACSS3_PONAB (Acyl-CoA synthetase short-chain family member 3, mitochondrial OS=Pongo abelii OX=9601 GN=ACSS3 PE=2 SV=1)

HSP 1 Score: 301.212 bits (770), Expect = 1.859e-97
Identity = 171/473 (36.15%), Postives = 263/473 (55.60%), Query Frame = 3 
Query:  249 KFIITTNFGYEPERTVNYKEIVDEAINKSQFCPDYCIVYNRPNTKCATMIDSRDLDWETEIGKATPYPPVALESGDPIFIIYTSGTLGEPKGLVMTVGGFVVSNKYFMKYVLGLNRRDCWWMFGDYGWISGQTTMTFGPLLIGCSTILYEGKELSSHHFTGIGRIIDQNNVTGFSAAPTYFRFLKNKDSEKDIKPSKAQLRF--VCIGGELSNTKLKEWVSNVFLCPVYDLWGQTELG--IIATGPGLGFKNISSNGLS----PMLSVHPKVVNSATGEQLPNETIGRMLLKIPLCPSAASSMYNDHDRFLKTYFSKXXXXXXXXXXXXIMNDGTLQILARADDVISVSAGRICGGALEEVCLMNEDLANAAFVALEDPINGQVPFAFLEKKKDRNINNEALIIESVKLVKKEIGSFTLFHQGVVVHDLPKTKTGKILRKTLYNMLNGQDWKTPATVENPKVFEEIYSILIEA 1643
            K ++T +FG EP R V Y  +V+EA+   Q  PD  ++YNRPN +   +   RDLDW+ E+ KA  +  V + S  P++I+YTSGT G PKG++   GG+ V   + M  + GL   + WW   D GW+ G + + +GPLL G +T+LYEGK + +       R++ ++ V     APT  R ++ +D    +    +  RF  + + GE  + +  EW  NVF  PV D W QTE G  I A+  GLG       G +    P  +V   ++     ++L    +G +++K+PL P A S ++ + + F   YF K+ GYYDTMD G +  +G + +++R DDVI+V+  RI  GA+EE  L +  +A+ A V  EDP+ G VP A    +KD N   E ++ E VK V++ IG    F   V V  LPKT++GKI R  L  ++NG+ +K  +T+E+P +F  +  +L +A
Sbjct:  217 KVVVTASFGIEPGRRVEYVPLVEEALKIGQHKPDKILIYNRPNMEAVPLAPGRDLDWDEEMAKAQSHDCVPVLSEHPLYILYTSGTTGLPKGVIRPTGGYAVMLNWSMSSIYGLQPGEVWWAASDLGWVVGHSYICYGPLLHGNTTVLYEGKPVGTPDAGAYFRVLAEHGVAALFTAPTAIRAIRQQDPGAALGKQYSLTRFKTLFVAGERCDVETLEWSKNVFRVPVLDHWWQTETGSPITASCVGLGNSKTPPPGQAGKSVPGYNV---MILDDNMQKLKARCLGNIVVKLPLPPGAFSGLWKNQEAFKHLYFEKFPGYYDTMDAGYMDEEGYVYVMSRVDDVINVAGHRISAGAIEESILSHGTVADCAVVGKEDPLKGHVPLALCVLRKDINATEEQVLEEIVKHVRQNIGPVAAFRNAVFVKQLPKTRSGKIPRSALSAIVNGKPYKITSTIEDPSIFGHVEEMLKQA 686

HSP 2 Score: 78.5666 bits (192), Expect = 1.859e-97
Identity = 37/84 (44.05%), Postives = 57/84 (67.86%), Query Frame = 2 
Query:    2 PVINATEKVSYKQLLTLVENLAGVMVNKCHVQKGDRVLLYVPVTVQGIAAILACTRIGAVYVVTFGGFAPDLIAARIKHTEPKI 253
            PV N     +YK++L  V  LAGV+V K  ++KGD V++Y+P+  Q +  +LAC RIGA++ + FGGFA   +++RI H +PK+
Sbjct:  136 PVTNTKATFTYKEVLEQVSKLAGVLV-KHGIKKGDTVVIYMPMIPQAMYTMLACARIGAIHSLIFGGFASKELSSRIDHVKPKV 218
[Close]
BLAST of Propionyl-CoA synthetase vs. UniProt/SwissProt
Match: sp|A7MB45|ACSS3_BOVIN (Acyl-CoA synthetase short-chain family member 3, mitochondrial OS=Bos taurus OX=9913 GN=ACSS3 PE=2 SV=1)

HSP 1 Score: 294.278 bits (752), Expect = 5.290e-96
Identity = 173/473 (36.58%), Postives = 261/473 (55.18%), Query Frame = 3 
Query:  249 KFIITTNFGYEPERTVNYKEIVDEAINKSQFCPDYCIVYNRPNTKCATMIDSRDLDWETEIGKATPYPPVALESGDPIFIIYTSGTLGEPKGLVMTVGGFVVSNKYFMKYVLGLNRRDCWWMFGDYGWISGQTTMTFGPLLIGCSTILYEGKELSSHHFTGIGRIIDQNNVTGFSAAPTYFRFLKNKDSEKDIKPSKAQLRF--VCIGGELSNTKLKEWVSNVFLCPVYDLWGQTELG--IIATGPGLGFKNISSNGLS----PMLSVHPKVVNSATGEQLPNETIGRMLLKIPLCPSAASSMYNDHDRFLKTYFSKXXXXXXXXXXXXIMNDGTLQILARADDVISVSAGRICGGALEEVCLMNEDLANAAFVALEDPINGQVPFAFLEKKKDRNINNEALIIESVKLVKKEIGSFTLFHQGVVVHDLPKTKTGKILRKTLYNMLNGQDWKTPATVENPKVFEEIYSILIEA 1643
            K +IT +FG EP R V Y  +V+EA+   Q  PD  ++YNRP+T    +     LDW+ E+ KA  +  V + S  P++I+YTSGT G PKG+V   GG+ V   + M  + GL   + WW   D GW+ G + + +GPLL G +T+LYEGK + +       R++ ++ V     APT  R ++ +D    +    +  RF  + + GE  + +  EW   VF  PV D W QTE G  I A+  GLG       G +    P  +V   ++     ++L    +G +++K+PL P A S ++ + + F   YF K+ GYYDTMD G +  +G + +++R DDVI+V+  RI  GALEE  L    +A+ A V  ED + G +P A    KKD N   E ++ E VK V++ IG    F + V V  LPKT++GKI R TL  ++NG+ +K   T+E+P +FE I ++L +A
Sbjct:  216 KLVITASFGIEPGRKVEYVPLVEEALRIGQHKPDKVLIYNRPHTDMVPLAPGYYLDWDEELSKAQSHDCVPVLSEHPLYILYTSGTTGLPKGVVRPTGGYAVMLNWSMSSIYGLKPGEVWWAASDLGWVVGHSYICYGPLLHGNTTVLYEGKPVGTPDAGAYFRVLAEHGVAALFTAPTAIRAIRQQDPGAALGKQYSLTRFKTLFVAGERCDVETLEWSKKVFRVPVLDHWWQTETGSPITASCIGLGNSKTPPPGQAGKSVPGYNV---MILDDNMQKLKARCLGNIVVKLPLPPGAFSGLWKNQEAFKHLYFEKFPGYYDTMDAGYMDEEGYVYVMSRVDDVINVAGHRISAGALEESILSLGIVADCAVVGKEDSLKGHIPLALCVLKKDINTTEEHVLEEIVKHVRQTIGPVAAFRKAVFVKQLPKTRSGKIPRSTLSALVNGKPYKVSPTIEDPGIFEHIEAMLKQA 685

HSP 2 Score: 80.8777 bits (198), Expect = 5.290e-96
Identity = 39/84 (46.43%), Postives = 58/84 (69.05%), Query Frame = 2 
Query:    2 PVINATEKVSYKQLLTLVENLAGVMVNKCHVQKGDRVLLYVPVTVQGIAAILACTRIGAVYVVTFGGFAPDLIAARIKHTEPKI 253
            PV N    ++YK++L  V  LAGV+V K  V+KGD V++Y+P+  Q + A+LAC RIGA++ + FGGFA   ++ RI H +PK+
Sbjct:  135 PVTNTKATITYKEVLEQVSKLAGVLV-KHGVKKGDTVVIYMPMIPQAMYAMLACARIGAIHSLIFGGFASKELSTRIDHAKPKL 217
[Close]
BLAST of Propionyl-CoA synthetase vs. TrEMBL
Match: J7JE35 (PrpE protein OS=Burkholderia cepacia GG4 OX=1009846 GN=GEM_5816 PE=4 SV=1)

HSP 1 Score: 320.087 bits (819), Expect = 5.975e-102
Identity = 177/482 (36.72%), Postives = 273/482 (56.64%), Query Frame = 3 
Query:  228 ELNIQSQKFIITTNFGYEPERTVNYKEIVDEAINKSQFCPDYCIVYNRPNTKCATMIDSRDLDWETEIGKATPYPPVALESGDPIFIIYTSGTLGEPKGLVMTVGGFVVSNKYFMKYVLGLNRRDCWWMFGDYGWISGQTTMTFGPLLIGCSTILYEGKELSSHHFTGIGRIIDQNNVTGFSAAPTYFRFLKNKDSEKDI--KPSKAQLRFVCIGGELSNTKLKEWVSNVFLCPVYDLWGQTELGIIATGPGLG---FKNISSNGLSPMLSVHPKVVNSATGEQLPNETIGRMLLKIPLCPSAASSMYNDHDRFLKTYFSKXXXXXXXXXXXXIMNDGTLQILARADDVISVSAGRICGGALEEVCLMNEDLANAAFVALEDPINGQVPFAFLEKKKDRNINNEALIIESVKLVKKEIGSFTLFHQGVVVHDLPKTKTGKILRKTLYNMLNGQDWKTPATVENPKVFEEIYSILIEAGYKAS 1658
             ++  + K I+  + G EP R V YK ++D AI++++  PD  IV+ RP    A M+D RD+DW T + +A P   V + + DP++I+YTSGT G+PKG+V   GG  V+ ++ M+Y   +   + +W   D GW+ G + + +GPLL GC+T+++EGK + +       R+I Q+ VT    APT FR +K +D +     K   +  R + + GE S+     W  +    PV D W QTE G    G  LG   F     +    M     +V+N    + +P   IG + +K+PL P A  +++N  +RF K+Y   Y GYY T D G I  DG + ++AR DD+I+V+  R+  GA+EEV   ++D+A  A + + D + GQVP  FL  K      +E ++ E V+LV+++IG    F + VVV  LPKT++GKILR T+  + NG+++KTPAT+++P+V  EI   L   GY  S
Sbjct:  157 RIDDATPKVIVAASCGIEPNRVVEYKPMLDAAIDQAEHKPDGVIVFQRPQA-IAPMVDGRDIDWNTAVAQALPADCVPVRATDPLYILYTSGTTGQPKGVVRDNGGHAVALRWTMEYFYDVQPGEVYWAASDVGWVVGHSYIVYGPLLAGCTTVVFEGKPVGTPDAGTFWRVIAQHKVTTLFTAPTAFRAIKREDPDARHLGKHDLSHFRALFLAGERSDPDTLRWAEDNLKVPVIDHWWQTETGWAVAGNPLGVHLFPIKYGSAARAMPGWDVRVLN-GNQDDVPRGDIGALCVKLPLPPGALPTLWNADERFKKSYLHAYSGYYQTGDAGFIDEDGYVHVMARTDDIINVAGHRLSTGAMEEVLANHKDVAECAVIGVADGLKGQVPLGFLCLKVGVMRPHEEIVKEVVQLVREQIGPVADFKRAVVVDRLPKTRSGKILRGTMQKIANGEEYKTPATIDDPEVLPEISKALHSGGYSKS 636

HSP 2 Score: 82.8037 bits (203), Expect = 5.975e-102
Identity = 39/84 (46.43%), Postives = 57/84 (67.86%), Query Frame = 2 
Query:    2 PVINATEKVSYKQLLTLVENLAGVMVNKCHVQKGDRVLLYVPVTVQGIAAILACTRIGAVYVVTFGGFAPDLIAARIKHTEPKI 253
            PV +  + ++Y +L  LV + AG +  +  V+KGDRVLLY+P+  Q + A+LAC R+GAV+ V FGGFAP+ +A RI    PK+
Sbjct:   83 PVTDTVQTITYAELQDLVAHFAGALRAQG-VEKGDRVLLYMPMIPQSVVAMLACARLGAVHSVVFGGFAPNELATRIDDATPKV 165
[Close]
BLAST of Propionyl-CoA synthetase vs. TrEMBL
Match: A0A235H791 (Propionyl-CoA synthetase OS=Azospirillum brasilense OX=192 GN=prpE PE=4 SV=1)

HSP 1 Score: 318.931 bits (816), Expect = 6.876e-100
Identity = 174/479 (36.33%), Postives = 275/479 (57.41%), Query Frame = 3 
Query:  228 ELNIQSQKFIITTNFGYEPERTVNYKEIVDEAINKSQFCPDYCIVYNRPNTKCATMIDSRDLDWETEIGKATPYPPVALESGDPIFIIYTSGTLGEPKGLVMTVGGFVVSNKYFMKYVLGLNRRDCWWMFGDYGWISGQTTMTFGPLLIGCSTILYEGKELSSHHFTGIGRIIDQNNVTGFSAAPTYFRFLKNKDSEKDI--KPSKAQLRFVCIGGELSNTKLKEWVSNVFLCPVYDLWGQTELGIIATGPGLG---FKNISSNGLSPMLSVHPKVVNSATGEQLPNETIGRMLLKIPLCPSAASSMYNDHDRFLKTYFSKXXXXXXXXXXXXIMNDGTLQILARADDVISVSAGRICGGALEEVCLMNEDLANAAFVALEDPINGQVPFAFLEKKKDRNINNEALIIESVKLVKKEIGSFTLFHQGVVVHDLPKTKTGKILRKTLYNMLNGQDWKTPATVENPKVFEEIYSILIEAGY 1649
             +N    K I++ + G EP R V YK ++D AI +++  P   IV+ RP  + AT+++ RD+DW   + KA P   V + + DP++I+YTSGT G+PKG++   GG  V+ K+ MK +  +   + +W   D GW+ G + + +GPLL GC+T+++EGK + +       R+I+Q+ +     APT FR +K +D   ++  K   +  R + + GE S+     W  +    PV D W QTE G   +G  LG   F     +   PM     +++N A  +++P   IG + +K+PL P A  +++N  DR+ K+Y S Y GYY T D G I +DG + I+AR DD+I+V+  R+  G +EEV   ++D+A  A + + D + GQVP  FL  K      +E ++ E V+LV+++IG    F + VVV  LPKT++GKILR T+  + + QD+KTPAT+++P +  EI   L   GY
Sbjct:  157 RINDSRPKAIVSASCGIEPNRVVKYKPMLDAAIEQAEHKPSSVIVFQRPQ-ETATLVEGRDVDWAEAVAKAEPAECVPVAATDPLYILYTSGTTGQPKGVIRDNGGHAVALKWTMKNIYNVEPGEVYWAASDVGWVVGHSYIVYGPLLHGCTTVVFEGKPVGTPDAGTFWRVIEQHRIGTLFTAPTAFRAIKREDPNAELLKKYDLSHFRALFLAGERSDPDTLHWAEDNLNVPVIDHWWQTETGWAISGNPLGVHLFPIKYGSATRPMPGWDVQILN-AENKEVPRGDIGAICVKLPLPPGALPTLWNADDRYRKSYLSDYPGYYQTGDAGFIDDDGYVYIMARTDDIINVAGHRLSTGGMEEVLASHKDVAECAVIGVADDLKGQVPLGFLCLKAGVTRPHEEIVKEVVQLVREQIGPVADFKRAVVVERLPKTRSGKILRGTMQKIADNQDYKTPATIDDPGILPEIAEALQSLGY 633

HSP 2 Score: 77.0258 bits (188), Expect = 6.876e-100
Identity = 37/83 (44.58%), Postives = 53/83 (63.86%), Query Frame = 2 
Query:    2 PVINATEKVSYKQLLTLVENLAGVMVNKCHVQKGDRVLLYVPVTVQGIAAILACTRIGAVYVVTFGGFAPDLIAARIKHTEPK 250
            PV    + ++Y +L   V   AG +  +  V+KGDRV+LY+P+  Q + A+LAC R+GAV+ V FGGFAP  +A RI  + PK
Sbjct:   83 PVTKTVQTITYGELQDQVARFAGALRAQG-VEKGDRVILYMPMIPQSLVAMLACARLGAVHSVVFGGFAPHELATRINDSRPK 164
[Close]
BLAST of Propionyl-CoA synthetase vs. TrEMBL
Match: A0A560AK80 (Propionyl-CoA synthetase OS=Azospirillum brasilense OX=192 GN=FBZ82_11916 PE=4 SV=1)

HSP 1 Score: 317.39 bits (812), Expect = 1.527e-99
Identity = 173/479 (36.12%), Postives = 274/479 (57.20%), Query Frame = 3 
Query:  228 ELNIQSQKFIITTNFGYEPERTVNYKEIVDEAINKSQFCPDYCIVYNRPNTKCATMIDSRDLDWETEIGKATPYPPVALESGDPIFIIYTSGTLGEPKGLVMTVGGFVVSNKYFMKYVLGLNRRDCWWMFGDYGWISGQTTMTFGPLLIGCSTILYEGKELSSHHFTGIGRIIDQNNVTGFSAAPTYFRFLKNKDSEKDI--KPSKAQLRFVCIGGELSNTKLKEWVSNVFLCPVYDLWGQTELGIIATGPGLG---FKNISSNGLSPMLSVHPKVVNSATGEQLPNETIGRMLLKIPLCPSAASSMYNDHDRFLKTYFSKXXXXXXXXXXXXIMNDGTLQILARADDVISVSAGRICGGALEEVCLMNEDLANAAFVALEDPINGQVPFAFLEKKKDRNINNEALIIESVKLVKKEIGSFTLFHQGVVVHDLPKTKTGKILRKTLYNMLNGQDWKTPATVENPKVFEEIYSILIEAGY 1649
             +N    K I++ + G EP R V YK ++D AI +++  P   IV+ RP  + AT+++ RD+DW   + KA P   V + + DP++I+YTSGT G+PKG++   GG  V+ K+ MK +  +   + +W   D GW+ G + + +GPLL GC+T+++EGK + +       R+I+Q+ +     APT FR +K +D   ++  K   +  R + + GE S+     W  +    PV D W QTE G   +G  LG   F     +   PM     +++N A  +++P   IG + +K+PL P    +++N  DR+ K+Y S Y GYY T D G I +DG + I+AR DD+I+V+  R+  G +EEV   ++D+A  A + + D + GQVP  FL  K      +E ++ E V+LV+++IG    F + VVV  LPKT++GKILR T+  + + QD+KTPAT+++P +  EI   L   GY
Sbjct:  157 RINDSRPKAIVSASCGIEPNRVVKYKPMLDAAIEQAEHKPSSVIVFQRPQ-ETATLVEGRDVDWAEAVAKAEPAECVPVAATDPLYILYTSGTTGQPKGVIRDNGGHAVALKWTMKNIYNVEPGEVYWAASDVGWVVGHSYIVYGPLLHGCTTVVFEGKPVGTPDAGTFWRVIEQHKIGTLFTAPTAFRAIKREDPNAELLKKYDLSHFRALFLAGERSDPDTLHWAEDNLNVPVIDHWWQTETGWAISGNPLGVHLFPIKYGSATRPMPGWDVRILN-AENKEVPRGDIGAICVKLPLPPGTLPTLWNADDRYRKSYLSDYPGYYQTGDAGFIDDDGYVYIMARTDDIINVAGHRLSTGGMEEVLASHKDVAECAVIGVADDLKGQVPLGFLCLKAGVTRPHEEIVKEVVQLVREQIGPVADFKRAVVVERLPKTRSGKILRGTMQKIADNQDYKTPATIDDPGILPEIAEALQSLGY 633

HSP 2 Score: 77.0258 bits (188), Expect = 1.527e-99
Identity = 37/83 (44.58%), Postives = 53/83 (63.86%), Query Frame = 2 
Query:    2 PVINATEKVSYKQLLTLVENLAGVMVNKCHVQKGDRVLLYVPVTVQGIAAILACTRIGAVYVVTFGGFAPDLIAARIKHTEPK 250
            PV    + ++Y +L   V   AG +  +  V+KGDRV+LY+P+  Q + A+LAC R+GAV+ V FGGFAP  +A RI  + PK
Sbjct:   83 PVTKTVQTITYAELQDQVARFAGALRAQG-VEKGDRVILYMPMIPQSLVAMLACARLGAVHSVVFGGFAPHELATRINDSRPK 164
[Close]
BLAST of Propionyl-CoA synthetase vs. TrEMBL
Match: A0A2K1FU22 (Propionyl-CoA synthetase OS=Azospirillum brasilense OX=192 GN=prpE PE=4 SV=1)

HSP 1 Score: 317.39 bits (812), Expect = 1.818e-99
Identity = 173/479 (36.12%), Postives = 274/479 (57.20%), Query Frame = 3 
Query:  228 ELNIQSQKFIITTNFGYEPERTVNYKEIVDEAINKSQFCPDYCIVYNRPNTKCATMIDSRDLDWETEIGKATPYPPVALESGDPIFIIYTSGTLGEPKGLVMTVGGFVVSNKYFMKYVLGLNRRDCWWMFGDYGWISGQTTMTFGPLLIGCSTILYEGKELSSHHFTGIGRIIDQNNVTGFSAAPTYFRFLKNKDSEKDI--KPSKAQLRFVCIGGELSNTKLKEWVSNVFLCPVYDLWGQTELGIIATGPGLG---FKNISSNGLSPMLSVHPKVVNSATGEQLPNETIGRMLLKIPLCPSAASSMYNDHDRFLKTYFSKXXXXXXXXXXXXIMNDGTLQILARADDVISVSAGRICGGALEEVCLMNEDLANAAFVALEDPINGQVPFAFLEKKKDRNINNEALIIESVKLVKKEIGSFTLFHQGVVVHDLPKTKTGKILRKTLYNMLNGQDWKTPATVENPKVFEEIYSILIEAGY 1649
             +N    K I++ + G EP R V YK ++D AI +++  P   IV+ RP  + AT+++ RD+DW   + KA P   V + + DP++I+YTSGT G+PKG++   GG  V+ K+ MK +  +   + +W   D GW+ G + + +GPLL GC+T+++EGK + +       R+I+Q+ +     APT FR +K +D   ++  K   +  R + + GE S+     W  +    PV D W QTE G   +G  LG   F     +   PM     +++N A  +++P   IG + +K+PL P    +++N  DR+ K+Y S Y GYY T D G I +DG + I+AR DD+I+V+  R+  G +EEV   ++D+A  A + + D + GQVP  FL  K      +E ++ E V+LV+++IG    F + VVV  LPKT++GKILR T+  + + QD+KTPAT+++P +  EI   L   GY
Sbjct:  157 RINDSRPKAIVSASCGIEPNRVVKYKPMLDAAIEQAEHKPSSVIVFQRPQ-ETATLVEGRDVDWAEAVAKAEPAECVPVAATDPLYILYTSGTTGQPKGVIRDNGGHAVALKWTMKNIYNVEPGEVYWAASDVGWVVGHSYIVYGPLLHGCTTVVFEGKPVGTPDAGTFWRVIEQHKIGTLFTAPTAFRAIKREDPNAELLKKYDLSHFRALFLAGERSDPDTLHWAEDNLNVPVIDHWWQTETGWAISGNPLGVHLFPIKYGSATRPMPGWDVQILN-AENKEVPRGDIGAICVKLPLPPGTLPTLWNADDRYRKSYLSDYPGYYQTGDAGFIDDDGYVYIMARTDDIINVAGHRLSTGGMEEVLASHKDVAECAVIGVADDLKGQVPLGFLCLKAGVTRPHEEIVKEVVQLVREQIGPVADFKRAVVVERLPKTRSGKILRGTMQKIADNQDYKTPATIDDPGILPEIAEALQSLGY 633

HSP 2 Score: 77.0258 bits (188), Expect = 1.818e-99
Identity = 37/83 (44.58%), Postives = 53/83 (63.86%), Query Frame = 2 
Query:    2 PVINATEKVSYKQLLTLVENLAGVMVNKCHVQKGDRVLLYVPVTVQGIAAILACTRIGAVYVVTFGGFAPDLIAARIKHTEPK 250
            PV    + ++Y +L   V   AG +  +  V+KGDRV+LY+P+  Q + A+LAC R+GAV+ V FGGFAP  +A RI  + PK
Sbjct:   83 PVTKTVQTITYAELQDQVARFAGALRAQG-VEKGDRVILYMPMIPQSLVAMLACARLGAVHSVVFGGFAPHELATRINDSRPK 164
[Close]
BLAST of Propionyl-CoA synthetase vs. TrEMBL
Match: A0A091UXS7 (Uncharacterized protein (Fragment) OS=Nipponia nippon OX=128390 GN=Y956_11088 PE=4 SV=1)

HSP 1 Score: 308.916 bits (790), Expect = 1.881e-99
Identity = 172/468 (36.75%), Postives = 263/468 (56.20%), Query Frame = 3 
Query:  249 KFIITTNFGYEPERTVNYKEIVDEAINKSQFCPDYCIVYNRPNTKCATMIDSRDLDWETEIGKATPYPPVALESGDPIFIIYTSGTLGEPKGLVMTVGGFVVSNKYFMKYVLGLNRRDCWWMFGDYGWISGQTTMTFGPLLIGCSTILYEGKELSSHHFTGIGRIIDQNNVTGFSAAPTYFRFLKNKDSEKDI--KPSKAQLRFVCIGGELSNTKLKEWVSNVFLCPVYDLWGQTELGIIATGPGLGFKNISS----NGLSPMLSVHPKVVNSATGEQLPNETIGRMLLKIPLCPSAASSMYNDHDRFLKTYFSKXXXXXXXXXXXXIMNDGTLQILARADDVISVSAGRICGGALEEVCLMNEDLANAAFVALEDPINGQVPFAFLEKKKDRNINNEALIIESVKLVKKEIGSFTLFHQGVVVHDLPKTKTGKILRKTLYNMLNGQDWKTPATVENPKVFEEIYSIL 1634
            K I+T +FG EP+R V Y  +++ A+   Q  PD  ++Y RPN     +   RDLDWE E+ KA  Y  V++ S  P++I+YTSGT G PKG++   GG+ V   + M  V GL   + WW   D GW+ G + + +GPLL G +T+LYEGK + +       R++ ++ V  F  +PT  R ++ +D E  +  + S  + R + + GE  +    EW   VF  PV D W QTE G   T   +G  N ++        P+   +  +++    E +  +T+G +++K+PL P A S ++ + + F K YF K+ GYYDTMD G +  DG L +L+RADDVI+V+  RI  GA+EE  L +  +A+ A V  EDP+ G VPFA    ++D     E ++ E V+ V+  IG    F +GV V  LPKT++GKI R  L  +++G+ +K   T+E+  VF+ I  +L
Sbjct:  113 KLIVTASFGVEPKRKVEYIALLEGALESVQSKPDKVLIYQRPNLGHVPLAPGRDLDWEEELAKAQCYKCVSVPSDHPLYILYTSGTTGSPKGVIRPTGGYAVMLNWTMSAVYGLKPGEVWWAASDLGWVVGHSYICYGPLLHGNTTVLYEGKPVGTPDAGAYFRVLAEHEVAAFFTSPTAIRAIRQQDPEAALGKQYSLKRFRTLFVAGERCDVDTLEWSKKVFKVPVLDHWWQTETGSAITASCIGLGNSTTPPPGQAGKPVPGYNVMILDE-NKEPVKAKTLGNIVVKLPLPPGAFSGLWENQETFQKLYFQKFPGYYDTMDAGYMDEDGYLYVLSRADDVINVAGHRISAGAIEECLLFHHAVADCAVVGQEDPLKGHVPFALCVLREDIKTEEEKILEEIVERVRTNIGPVAAFQKGVFVKQLPKTRSGKIPRSALSALVSGKPYKITPTIEDAGVFKHIEEVL 579

HSP 2 Score: 85.1149 bits (209), Expect = 1.881e-99
Identity = 41/84 (48.81%), Postives = 59/84 (70.24%), Query Frame = 2 
Query:    2 PVINATEKVSYKQLLTLVENLAGVMVNKCHVQKGDRVLLYVPVTVQGIAAILACTRIGAVYVVTFGGFAPDLIAARIKHTEPKI 253
            PV N  EK++YK+LL  V  LA VMV +  V+KGDRV++Y+P+  Q +  +LAC RIGA++ + FGGFA   ++ RI H +PK+
Sbjct:   32 PVTNTKEKITYKELLEQVSKLADVMV-RHGVKKGDRVVIYMPMIPQTVYCMLACARIGAIHSLIFGGFASKELSVRIDHAKPKL 114
[Close]
BLAST of Propionyl-CoA synthetase vs. Ensembl Cavefish
Match: acss2 (acyl-CoA synthetase short chain family member 2 [Source:NCBI gene;Acc:103041660])

HSP 1 Score: 181.8 bits (460), Expect = 4.178e-57
Identity = 146/500 (29.20%), Postives = 238/500 (47.60%), Query Frame = 3 
Query:  222 LLELNIQSQ-KFIITTNFGYEPERTVNYKEIVDEAINKSQ---FCPDYCIVYNRPNTKCATMIDSRDLD-------------WETEI---------GKATPYPPVALESGDPIFIIYTSGTLGEPKGLVMTVGGFVVSNKYFMKYVLGLNRRDCWWMFGDYGWISGQTTMTFGPLLIGCSTILYEGKELSSH-HFTGIGRIIDQNNVTGFSAAPTYFRFLKNKDSEKDIKPSKAQLRFVCIGGELSNTKLKEWVSNVF---LCPVYDLWGQTELGIIATGPGLGFKNISSNGLS-PMLSVHPKVVNSATGEQLPNETIGRMLLKIPLCPSAASSMYNDHDRFLKTYFSKXXXXXXXXXXXXIMNDGTLQILARADDVISVSAGRICGGALEEVCLMNEDLANAAFVALEDPINGQVPFAFLEKKKDRNINNEALIIESVKLVKKEIGSFTLFHQGVVVHDLPKTKTGKILRKTLYNMLNGQ-DWKTPATVENPKVFEEIY 1625
            L E  I S+   +IT +  Y  ++ +N K I DEA+ K +   F  + CIV    + +    +    L              W+ E+         G +    PV  ES DP+FI+YTSG+ G+PKG++ TV G+++      K V   +  D +W   D GWI+G + +T+GPL  G +++L+EG  L ++   + +  I+D+ +V+ F  APT  R L    ++   K  ++ L+ +   GE  N +  +W  NV     CPV D + QTE G     P      +     + P   V P ++N  +GE+L     G ++ K P  P    +++ +H RF  TYF K+ GYY T D      DG   I  R DD+++VS   +    +E   + +E +A AA V    P+ G+  + F+      N     L  E  K V+++IG+            LPKT++GKI+R+ L  + + Q D    +T+ +  V E+++
Sbjct:  182 LCERIIDSRCSLLITADGFYRGDKLINLKLIADEALQKCRDKSFPVERCIVLKHLSKEEHESLSLGSLSPPAKRSCPDLQVPWKPEVDVCWHSLMEGASDECEPVWCESEDPLFILYTSGSTGKPKGVLHTVSGYMLYTATTFKLVFDYHPDDIYWCTADIGWITGHSYITYGPLANGATSVLFEG--LPTYPDVSRMWEIVDKYHVSKFYTAPTAIRLLMKYGNDPVHKYKRSSLKILGTVGEPINPEAWQWYYNVVGEGRCPVVDTFWQTETGGHVLTPLPAATPLKPGSATFPFFGVVPAILNE-SGEELEGPNEGYLVFKQPW-PGVMRTVFGNHQRFETTYFKKFPGYYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALVEHEAVAEAAVVGTPHPVKGESLYCFVTLADGMNYTT-TLEAELKKQVREKIGAIATPDYIQNAPGLPKTRSGKIMRRLLRKIASNQRDLGDVSTLADTSVIEQLF 676

HSP 2 Score: 60.4622 bits (145), Expect = 4.178e-57
Identity = 30/69 (43.48%), Postives = 48/69 (69.57%), Query Frame = 2 
Query:   26 VSYKQLLTLVENLAGVMVNKCHVQKGDRVLLYVPVTVQGIAAILACTRIGAVYVVTFGGFAPDLIAARI 232
            V+Y++LL  V   A V+ ++  V+KGDRV +Y+P+ V+ + A+LAC RIGAV+ + F GF+ + +  RI
Sbjct:  119 VTYRELLQQVCKFANVLKSQ-GVKKGDRVSIYMPMVVELVVAMLACARIGAVHSIVFAGFSAESLCERI 186
[Close]
BLAST of Propionyl-CoA synthetase vs. Ensembl Cavefish
Match: acss2 (acyl-CoA synthetase short chain family member 2 [Source:NCBI gene;Acc:103041660])

HSP 1 Score: 177.948 bits (450), Expect = 5.923e-56
Identity = 148/516 (28.68%), Postives = 240/516 (46.51%), Query Frame = 3 
Query:  222 LLELNIQSQ-KFIITTNFGYEPERTVNYKEIVDEAINKSQ---FCPDYCIVYNR---------------PNTK--CATMIDSRD------------LDWETEI---------GKATPYPPVALESGDPIFIIYTSGTLGEPKGLVMTVGGFVVSNKYFMKYVLGLNRRDCWWMFGDYGWISGQTTMTFGPLLIGCSTILYEGKELSSH-HFTGIGRIIDQNNVTGFSAAPTYFRFLKNKDSEKDIKPSKAQLRFVCIGGELSNTKLKEWVSNVF---LCPVYDLWGQTELGIIATGPGLGFKNISSNGLS-PMLSVHPKVVNSATGEQLPNETIGRMLLKIPLCPSAASSMYNDHDRFLKTYFSKXXXXXXXXXXXXIMNDGTLQILARADDVISVSAGRICGGALEEVCLMNEDLANAAFVALEDPINGQVPFAFLEKKKDRNINNEALIIESVKLVKKEIGSFTLFHQGVVVHDLPKTKTGKILRKTLYNMLNGQ-DWKTPATVENPKVFEEIY 1625
            L E  I S+   +IT +  Y  ++ +N K I DEA+ K +   F  + CIV                  P  K  C  +   +             + W+ E+         G +    PV  ES DP+FI+YTSG+ G+PKG++ TV G+++      K V   +  D +W   D GWI+G + +T+GPL  G +++L+EG  L ++   + +  I+D+ +V+ F  APT  R L    ++   K  ++ L+ +   GE  N +  +W  NV     CPV D + QTE G     P      +     + P   V P ++N  +GE+L     G ++ K P  P    +++ +H RF  TYF K+ GYY T D      DG   I  R DD+++VS   +    +E   + +E +A AA V    P+ G+  + F+      N     L  E  K V+++IG+            LPKT++GKI+R+ L  + + Q D    +T+ +  V E+++
Sbjct:  182 LCERIIDSRCSLLITADGFYRGDKLINLKLIADEALQKCRDKSFPVERCIVLKHLSKEEHESLSLGSLSPPAKRSCPDLQQEKQKGNVRKVRSIPKVPWKPEVDVCWHSLMEGASDECEPVWCESEDPLFILYTSGSTGKPKGVLHTVSGYMLYTATTFKLVFDYHPDDIYWCTADIGWITGHSYITYGPLANGATSVLFEG--LPTYPDVSRMWEIVDKYHVSKFYTAPTAIRLLMKYGNDPVHKYKRSSLKILGTVGEPINPEAWQWYYNVVGEGRCPVVDTFWQTETGGHVLTPLPAATPLKPGSATFPFFGVVPAILNE-SGEELEGPNEGYLVFKQPW-PGVMRTVFGNHQRFETTYFKKFPGYYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALVEHEAVAEAAVVGTPHPVKGESLYCFVTLADGMNYTT-TLEAELKKQVREKIGAIATPDYIQNAPGLPKTRSGKIMRRLLRKIASNQRDLGDVSTLADTSVIEQLF 692

HSP 2 Score: 60.8474 bits (146), Expect = 5.923e-56
Identity = 30/69 (43.48%), Postives = 48/69 (69.57%), Query Frame = 2 
Query:   26 VSYKQLLTLVENLAGVMVNKCHVQKGDRVLLYVPVTVQGIAAILACTRIGAVYVVTFGGFAPDLIAARI 232
            V+Y++LL  V   A V+ ++  V+KGDRV +Y+P+ V+ + A+LAC RIGAV+ + F GF+ + +  RI
Sbjct:  119 VTYRELLQQVCKFANVLKSQG-VKKGDRVSIYMPMVVELVVAMLACARIGAVHSIVFAGFSAESLCERI 186
[Close]
BLAST of Propionyl-CoA synthetase vs. Ensembl Cavefish
Match: acss2l (acetyl-coenzyme A synthetase, cytoplasmic-like [Source:NCBI gene;Acc:107197877])

HSP 1 Score: 172.94 bits (437), Expect = 3.214e-53
Identity = 151/502 (30.08%), Postives = 241/502 (48.01%), Query Frame = 3 
Query:  222 LLELNIQSQKFIITTNFG-YEPERTVNYKEIVDEAIN----KSQFCPDYCIVY---------NRPNTKCATMIDSR-DLDWETEI-GKATPYPPVALESGDPIFIIYTSGTLGEPKGLVMTVGGFVVSNKYFMKYVLGLNRRDCWWMFGDYGWISGQTTMTFGPLLIGCSTILYEGKELSSHHFTGIGR---IIDQNNVTGFSAAPTYFRFLKNKDSEKDIKPSKAQLRFVCIGGELSNTKLKEWVSNVF---LCPVYDLWGQTELG--IIATGPGLGFKNISSNGLSPMLSVHPKVVNSATGEQLPNETIGRMLLKIPLCPSAASSMYNDHDRFLKTYFSKXXXXXXXXXXXXIMNDGTLQILARADDVISVSAGRICGGALEEVCLMNEDLANAAFVALEDPINGQVPFAFLEKKKDRNINNEALIIESVKLVKKEIGSFTL--FHQGVVVHDLPKTKTGKILRKTLYNML-NGQDWKTPATVENPKVFEEIYSILIEAG 1646
            L E  + +Q  ++ T  G Y  ++ +  K+I DEA++    K+      C+V          N    K  T+ ++  D+ W+  + G +    P  +E+ DP+FI+YTSG+ G+PKG++ T+ G+++      KYV   +R D +W   D GWI+G + +T+GPL  G +++L+EG  +  H    +GR   II++  V+ F  APT  R L     E   K   + LR +   GE  N +   W  +      CPV D + QTE G  ++   P        S  L P   V P ++N    E    E  G ++ + P  P     +Y + +RF  TYF K+ G+Y T D      DG   I  R DD+++VS   +    +E     +  +A AA V+    I G+  + F+  K  R  N+  L+ E  + V+++IG      F Q      LPKT++GKI+R+ L  +  N +D    +T+ +PKV E + S   EA 
Sbjct:  184 LAERIMDAQCTVLVTADGVYRGDKLIKLKQISDEALDHCREKNSLTVRKCLVVKHHAFRIHGNDTCNKLQTLWNAECDVWWDEAVEGVSDECDPEWMEAEDPLFILYTSGSTGKPKGVLHTIAGYLLYTSLTFKYVFDHHRDDVYWCTADIGWITGHSYITYGPLANGATSVLFEGIPVHPH----VGRFWEIIEKYRVSKFYTAPTAIRLLMKYGREPLSKYDLSSLRVLGTVGEPINPEAWLWYHDAVGQGRCPVVDTFWQTETGGHVLTPLPAATPLKPGSATL-PFFGVLPIILNEHGEELE-GEAEGYLVFQKPW-PGIMRGVYGNQERFENTYFKKFPGFYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTSEVEAALSEHSAVAEAAVVSRPHTIKGECLYCFVTLKDCREFNH-TLLEELKRKVREKIGPIATPDFIQNAPA--LPKTRSGKIMRRVLRQIARNEKDLGDLSTLADPKVVEVLLSQRCEAA 675

HSP 2 Score: 56.6102 bits (135), Expect = 3.214e-53
Identity = 28/69 (40.58%), Postives = 45/69 (65.22%), Query Frame = 2 
Query:   26 VSYKQLLTLVENLAGVMVNKCHVQKGDRVLLYVPVTVQGIAAILACTRIGAVYVVTFGGFAPDLIAARI 232
            ++Y QLL  V   A  +  +  V+KGDRV +Y+P+  + + ++LAC RIGAV+ + F GF+ + +A RI
Sbjct:  121 ITYSQLLKQVCRCANAL-KQMGVKKGDRVAIYLPMIPELVYSMLACARIGAVHSIVFAGFSSESLAERI 188
[Close]
BLAST of Propionyl-CoA synthetase vs. Ensembl Cavefish
Match: acss2l (acetyl-coenzyme A synthetase, cytoplasmic-like [Source:NCBI gene;Acc:107197877])

HSP 1 Score: 160.999 bits (406), Expect = 9.346e-50
Identity = 145/495 (29.29%), Postives = 238/495 (48.08%), Query Frame = 3 
Query:  255 IITTNFGYEPERTVNYKEIVDEAIN----KSQFCPDYCIVY---------NRPNTKCATMIDSR-DLDWETEI-GKATPYPPVALESGDPIFIIYTSGTLGEPKGLVMTVGGFVVSNKYFMKYVLGLNRRDCWWMFGDYGWISGQTTMTFGPLLIGCSTILYEGKELSSHHFTGIGR---IIDQNNVTGFSAAPTYFRFLKNKDSEKDIKPSKAQLRFVCIGGELSNTKLKEWVSNVF---LCPVYDLWGQT----ELGIIATGPGLGFKNISSNGLSPMLSVHPKVVNSATGEQLPNETIGRMLLKIPLCPSAASSMYNDHDRFLKTYFSKXXXXXXXXXXXXIMNDGTLQILARADDVISVSAGRICGGALEEVCLMNEDLANAAFVALEDPINGQVPFAFLEKKKDRNINNEALIIESVKL---VKKEIGSFTL--FHQGVVVHDLPKTKTGKILRKTLYNML-NGQDWKTPATVENPKVFEEIYSILIEAG 1646
            ++T +  Y  ++ +  K+I DEA++    K+      C+V          N    K  T+ ++  D+ W+  + G +    P  +E+ DP+FI+YTSG+ G+PKG++ T+ G+++      KYV   +R D +W   D GWI+G + +T+GPL  G +++L+EG  +  H    +GR   II++  V+ F  APT  R L     E       + LR +   GE  N +   W  +      CPV D + QT    E+ I+ +     F+ I+     P   V P ++N    E    E  G ++ + P  P     +Y + +RF  TYF K+ G+Y T D      DG   I  R DD+++VS   +    +E     +  +A AA V+    I G+  + F+  K  R  N+   ++E +K    V+++IG      F Q      LPKT++GKI+R+ L  +  N +D    +T+ +PKV E + S   EA 
Sbjct:  196 LVTADGVYRGDKLIKLKQISDEALDHCREKNSLTVRKCLVVKHHAFRIHGNDTCNKLQTLWNAECDVWWDEAVEGVSDECDPEWMEAEDPLFILYTSGSTGKPKGVLHTIAGYLLYTSLTFKYVFDHHRDDVYWCTADIGWITGHSYITYGPLANGATSVLFEGIPVHPH----VGRFWEIIEKYRVSKFYTAPTAIRLLMKYGRE-----PLSNLRVLGTVGEPINPEAWLWYHDAVGQGRCPVVDTFWQTETVSEIHIMVSRH--NFRIIT----LPFFGVLPIILNEHGEELE-GEAEGYLVFQKPW-PGIMRGVYGNQERFENTYFKKFPGFYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTSEVEAALSEHSAVAEAAVVSRPHTIKGECLYCFVTLKDCREFNH--TLLEELKRKGQVREKIGPIATPDFIQNAPA--LPKTRSGKIMRRVLRQIARNEKDLGDLSTLADPKVVEVLLSQRCEAA 669

HSP 2 Score: 56.6102 bits (135), Expect = 9.346e-50
Identity = 28/69 (40.58%), Postives = 45/69 (65.22%), Query Frame = 2 
Query:   26 VSYKQLLTLVENLAGVMVNKCHVQKGDRVLLYVPVTVQGIAAILACTRIGAVYVVTFGGFAPDLIAARI 232
            ++Y QLL  V   A  +  +  V+KGDRV +Y+P+  + + ++LAC RIGAV+ + F GF+ + +A RI
Sbjct:  121 ITYSQLLKQVCRCANAL-KQMGVKKGDRVAIYLPMIPELVYSMLACARIGAVHSIVFAGFSSESLAERI 188
[Close]
BLAST of Propionyl-CoA synthetase vs. Ensembl Cavefish
Match: acss1 (acyl-CoA synthetase short chain family member 1 [Source:ZFIN;Acc:ZDB-GENE-050320-139])

HSP 1 Score: 107.842 bits (268), Expect = 2.842e-37
Identity = 85/316 (26.90%), Postives = 144/316 (45.57%), Query Frame = 3 
Query:  249 KFIITTNFGYEPERTVNYKEIVDEAINKSQFCP--DYCIVYNRPNTKCATMIDSRDLDWETEIGKATPYPPVALESGDPIFIIYTSGTLGEPKGLVMTVGGFVVSNKYFMKYVLGLNRRDCWWMFGDYGWISGQTTMTFGPLLIGCSTILYEGKELSSHHFTGIGR---IIDQNNVTGFSAAPTYFRFLKNKDSEKDIKPSKAQLRFVCIGGELSNTKLKEWVSNVF---LCPVYDLWGQTELGIIATGPGLGFKNISSNGLSPMLSVHPKVVNSATGEQLP-NETIGRMLLKIPLCPSAASSMYNDHDRFLKTYF 1169
            K ++T N G    R +N K  VD A+     CP  ++ +V  R N           L+ E    +++   P  ++S + +F++YTSG+ G+PKG+V T  G+++      +YV   +  D +    D GWI+G + + +GPL  G +T+L+E    S+  +   GR   ++++  +  F  APT  R L   +     K  ++ L+ +   GE  N +  EW  NV     CP+ D W               + ++  +G   M  V      S  G+ L  N+  G + +  P  P  A +++ DH RF+  YF
Sbjct:  212 KVVVTCNQGVRGGRLINLKATVDSAVKN---CPTVEHVLVAQRTNHSVPMGKLDTPLE-EAMARESSECAPEPMDSEEMLFMLYTSGSTGKPKGIVHTQAGYLLYASLTHQYVFDYSPGDVYGCVADIGWITGHSYVVYGPLCNGATTVLFE----STPVYPDPGRYWEMVERLRINQFYGAPTAIRLLLKYEESWVKKYDRSSLKTLGSVGEPINHEAWEWFHNVVGDGRCPLVDTW---------------WVSLFLHGCISMGCV------SECGQMLTGNDVTGALCISKPW-PGMAKTIFGDHQRFVDAYF 497

HSP 2 Score: 68.1662 bits (165), Expect = 2.842e-37
Identity = 32/78 (41.03%), Postives = 51/78 (65.38%), Query Frame = 2 
Query:   20 EKVSYKQLLTLVENLAGVMVNKCHVQKGDRVLLYVPVTVQGIAAILACTRIGAVYVVTFGGFAPDLIAARIKHTEPKI 253
            E+V+Y++LL +   +A  +     VQKGDRV +Y+PV+   +A +L C RIGAV+ V F GF+ + +A RI+  + K+
Sbjct:  137 ERVTYRELLEMTCRIANTL-KSLGVQKGDRVAIYMPVSPIAVATMLGCARIGAVHTVVFAGFSSEALAGRIQDAQCKV 213
[Close]
BLAST of Propionyl-CoA synthetase vs. Ensembl Sea Lamprey
Match: ENSPMAT00000006895.1 (pep scaffold:Pmarinus_7.0:GL476378:330410:368976:-1 gene:ENSPMAG00000006232.1 transcript:ENSPMAT00000006895.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 155.992 bits (393), Expect = 8.225e-55
Identity = 96/328 (29.27%), Postives = 165/328 (50.30%), Query Frame = 3 
Query:  225 LELNIQSQKFIITTNFGYEPERTVNYKEIVDEAINKSQFCPDYCIVYNRPNTKCATMIDSRDLDWETEIGKATPYPPVALESGDPIFIIYTSGTLGEPKGLVMTVGGFVVSNKYFMKYVLGLNRRDCWWMFGDYGWISGQTTMTFGPLLIGCSTILYEGKELSSHHFTGIGRIIDQN--NVTGFSAAPTYFRFLKNKDSEKDIK-----PSKAQLRFVCIGGELSNTKLKEWVSNVFLCPVYDLWGQTELG--IIATGPGLG---FKNISSNGLS-PMLSVHPKVVNSATGEQLPNETIGRMLLKIPLCPSAASSMYNDHDRFLKTYF 1169
            + +N      +++ +FG EP R V YK +++ A++ +   P+  +VY+RP    A++I  RD+ W+ E+  A P+  V + S  P++I+YTSGT G PKG+V   GG  V   + M  + GL   + WW   D GW+ G + + +GPLL G +T+LYE     S        + +++  N+    +  +++  L   + +         P   + R + + GE+ + +   W    F  P+ D W QTE G  I AT  GLG   +      G S P   V  +VV+S   E      +G +++K+PL P   ++++ ++ ++  TYF
Sbjct:  148 VRINHAKVSMVLSASFGIEPGRQVAYKPLLEAALDIATHKPEKVLVYSRPGMARASLIPGRDMCWQEEVSAAKPHDCVPVSSDHPLYILYTSGTTGAPKGVVRPTGGHAVMLNWSMSAIYGLKPGEVWWAASDLGWVVGHSYICYGPLLHGNTTVLYEPAPPVSPPPKVFYEVDEKHGLNLCFIPSPRSHYISLPMSNPQLLYHLYIKTPCVPRFRSMFVAGEICDKETLNWGKATFNVPLLDHWWQTETGSAITATCTGLGGSLYPPAGQCGKSVPGYDV--RVVDSNNQES-KRGNLGNIVVKLPLAPGCMATLWENNQKYEDTYF 472

HSP 2 Score: 76.2554 bits (186), Expect = 8.225e-55
Identity = 38/81 (46.91%), Postives = 55/81 (67.90%), Query Frame = 2 
Query:    2 PVINATEKVSYKQLLTLVENLAGVMVNKCHVQKGDRVLLYVPVTVQGIAAILACTRIGAVYVVTFGGFAPDLIAARIKHTE 244
            PV    + ++Y+Q L+ V  LA V+V K  VQKGDRV++Y+P+  Q + A+LAC RIGA++ + FGGFA   +A RI H +
Sbjct:   75 PVTGIIQTITYRQALSEVTRLASVLV-KNGVQKGDRVVIYMPMIPQAMFAMLACARIGAIHSLIFGGFASKELAVRINHAK 154
[Close]
BLAST of Propionyl-CoA synthetase vs. Ensembl Sea Lamprey
Match: acss2 (acyl-CoA synthetase short chain family member 2 [Source:ZFIN;Acc:ZDB-GENE-040718-388])

HSP 1 Score: 169.088 bits (427), Expect = 9.560e-47
Identity = 129/428 (30.14%), Postives = 205/428 (47.90%), Query Frame = 3 
Query:  384 CATMI---DSRDLDW-ETEIGKATPYPPVALESGDPIFIIYTSGTLGEPKGLVMTVGGFVVSNKYFMKYVLGLNRRDCWWMFGDYGWISGQTTMTFGPLLIGCSTILYEGKELSSHHFTGIGRI---IDQNNVTGFSAAPTYFRFLKNKDSEKDIKPSKAQLRFVCIGGELSNTKLKEWVSNVF---LCPVYDLWGQTELG--IIATGPGLGFKNISSNGLSPMLSVHPKVVNSATGEQLPNETIGRMLLKIPLCPSAASSMYNDHDRFLKTYFSKXXXXXXXXXXXXIMNDGTLQILARADDVISVSAGRICGGALEEVCLMNEDLANAAFVALEDPINGQVPFAFLEKKKDRNINNEALIIESVKLVKKEIGSFTLFHQGVVVHDLPKTKTGKILRKTLYNML-NGQDWKTPATVENPKVFEEIYS 1628
            C T +     RD  W E   G +T   P   ++ DP+F++YTSG+ G+PKG++ T  G+++      K V  +  +D +W   D GWI+G + + +GPL  G +++L+EG       F   GR    +++  V+ F  APT  R L        +K S+  LR +   GE  N +   W+  V     CP+ D + QTE G  ++   PG       S  L P   V P +++   GE+L  E  G ++ K P  P    +++ DH+R   TYF K+ GYY T D      DG   I  R DD+++VS   +    +E   L +  +A AA V     + G+  + F+       + N  L  E  K V+++IG             LPKT++GKI+R+ L  +  +  D+   +T+ +P V + +YS
Sbjct:    2 CLTQVPWTSGRDSWWHELMCGASTTCEPAWCDAEDPLFVLYTSGSTGKPKGVLHTTAGYLLFVATTHKLVFDIRPQDVYWCTADIGWITGHSYIVYGPLANGVTSVLFEGIPT----FPDAGRFWQTVEKYAVSKFYTAPTAVRLLMKHGDAFVLKHSRRSLRVLGTVGEPINREAWHWLHGVVGGGRCPIVDTFWQTETGGHVLTPLPGATPLKPGSATL-PFFGVEPAILDE-RGEELRGEAEGFLVFKRPW-PGILRTVFGDHERLEATYFGKFPGYYATGDGCRRDKDGYYWITGRTDDMLNVSGHLLSTAEVESALLSHGSVAEAAVVGRPHALKGESLYCFVTLNHGLEL-NPCLAAELKKRVREKIGPIATPDFIQSAPSLPKTRSGKIMRRLLRKIAQDDSDFGDLSTLADPGVVQLLYS 421
[Close]
BLAST of Propionyl-CoA synthetase vs. Ensembl Sea Lamprey
Match: ENSPMAT00000005866.1 (pep scaffold:Pmarinus_7.0:GL495638:310:2296:-1 gene:ENSPMAG00000005307.1 transcript:ENSPMAT00000005866.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 45.8246 bits (107), Expect = 1.347e-6
Identity = 23/62 (37.10%), Postives = 40/62 (64.52%), Query Frame = 2 
Query:   23 KVSYKQLLTLVENLAGVMVNKCHVQKGDRVLLYVPVTVQGIAAILACTRIGAVYVVTFGGFA 208
            +++YK+LL  V   + V+++   V KGDRV +Y+P+  + + A+LAC R+GAV+ +     A
Sbjct:   37 QITYKELLDQVCKCSNVLLSH-GVGKGDRVAIYMPMITELVVAMLACARVGAVHSIVVSSRA 97
[Close]
BLAST of Propionyl-CoA synthetase vs. Ensembl Yeast
Match: ACS1 (Acetyl-coA synthetase isoform; along with Acs2p, acetyl-coA synthetase isoform is the nuclear source of acetyl-coA for histone acetylation; expressed during growth on nonfermentable carbon sources and under aerobic conditions [Source:SGD;Acc:S000000050])

HSP 1 Score: 214.157 bits (544), Expect = 1.222e-66
Identity = 154/481 (32.02%), Postives = 241/481 (50.10%), Query Frame = 3 
Query:  228 ELNIQSQKFIITTNFGYEPERTVNYKEIVDEAINKSQFCPDYCIVYNRPNTKCATMIDSRDLDWETEIGK-ATPYPPVALESGDPIFIIYTSGTLGEPKGLVMTVGGFVVSNKYFMKYVLGLNRRDCWWMFGDYGWISGQTTMTFGPLLIGCSTILYEGKELSSHHFTGIGRIIDQNNVTGFSAAPTYFRFLKNKDSEKDIKPSKAQLRFVCIG--GELSNTKLKEWVSNVF---LCPVYDLWGQTELGIIATGPGLGFKNISSNGLS--PMLSVHPKVVNSATGEQLPNETIGRMLLKIPLCPSAASSMYNDHDRFLKTYFSKXXXXXXXXXXXXIMNDGTLQILARADDVISVSAGRICGGALEEVCLMNEDLANAAFVALEDPINGQVPFAFLEKKKDRN----INNEALIIES--VKLVKKEIGSFTLFHQGVVVHDLPKTKTGKILRKTLYNMLNGQDWK--TPATVENPKVFEEI 1622
             +N    K +ITT+      + +  K IVD+A+ ++     + +VY + N         RDLDW TE  K  T YP   ++S DP+F++YTSG+ G PKG+  +  G+++     M+Y    ++ D ++  GD GWI+G T + +GPLL GC+T+++EG   +  +++    IID++ VT F  APT  R LK          S   LR  C+G  GE    ++ EW S        P+ D + QTE G     P  G       G +  P   +   V++  TGE+L       +L      PS A +++ +HDR+L TY + Y GYY T D      DG + IL R DDV++VS  R+    +E   + +  +A  A V   D + GQ   AF+  K   +     ++E   I+   V  V+K+IG F      ++V DLPKT++GKI+R+ L  +L G+  +    +T+ NP +   +
Sbjct:  231 RINDGDSKVVITTDESNRGGKVIETKRIVDDALRETPGV-RHVLVYRKTNNPSVAFHAPRDLDWATEKKKYKTYYPCTPVDSEDPLFLLYTSGSTGAPKGVQHSTAGYLLGALLTMRYTFDTHQEDVFFTAGDIGWITGHTYVVYGPLLYGCATLVFEGTP-AYPNYSRYWDIIDEHKVTQFYVAPTALRLLKRAGDSYIENHSLKSLR--CLGSVGEPIAAEVWEWYSEKIGKNEIPIVDTYWQTESGSHLVTPLAGGVTPMKPGSASFPFFGIDAVVLDPNTGEELNTSHAEGVLAVKAAWPSFARTIWKNHDRYLDTYLNPYPGYYFTGDGAAKDKDGYIWILGRVDDVVNVSGHRLSTAEIEAAIIEDPIVAECAVVGFNDDLTGQAVAAFVVLKNKSSWSTATDDELQDIKKHLVFTVRKDIGPFAAPKLIILVDDLPKTRSGKIMRRILRKILAGESDQLGDVSTLSNPGIVRHL 707

HSP 2 Score: 57.3806 bits (137), Expect = 1.222e-66
Identity = 28/76 (36.84%), Postives = 47/76 (61.84%), Query Frame = 2 
Query:   26 VSYKQLLTLVENLAGVMVNKCHVQKGDRVLLYVPVTVQGIAAILACTRIGAVYVVTFGGFAPDLIAARIKHTEPKI 253
            ++YK+LL  V  +A V+     V+KGD V +Y+P+  + I  +LA +RIGA++ V F GF+ + +  RI   + K+
Sbjct:  164 ITYKELLEEVCQVAQVLTYSMGVRKGDTVAVYMPMVPEAIITLLAISRIGAIHSVVFAGFSSNSLRDRINDGDSKV 239
[Close]
BLAST of Propionyl-CoA synthetase vs. Ensembl Yeast
Match: ACS2 (Acetyl-coA synthetase isoform; along with Acs1p, acetyl-coA synthetase isoform is the nuclear source of acetyl-coA for histone acetylation; mutants affect global transcription; required for growth on glucose; expressed under anaerobic conditions [Source:SGD;Acc:S000004143])

HSP 1 Score: 209.92 bits (533), Expect = 1.274e-63
Identity = 152/486 (31.28%), Postives = 238/486 (48.97%), Query Frame = 3 
Query:  243 SQKFIITTNFGYEPERTVNYKEIVDEAINKSQFCPDYCIVYNRPNTKCATMIDSRDLDWETEIGKATPY-PPVALESGDPIFIIYTSGTLGEPKGLVMTVGGFVVSNKYFMKYVLGLNRRDCWWMFGDYGWISGQTTMTFGPLLIGCSTILYEGKELSSHHFTGIG---RIIDQNNVTGFSAAPTYFRFLKNKDSEKDIKPSKAQLRFVCIGGELSNTKLKEWVSNVF---LCPVYDLWGQTELGIIATGPGLG-FKNISSNGLSPMLSVHPKVVNSATGEQLP-NETIGRMLLKIPLCPSAASSMYNDHDRFLKTYFSKXXXXXXXXXXXXIMNDGTLQILARADDVISVSAGRICGGALEEVCLMNEDLANAAFVALEDPINGQVPFAFLEKKKDRNINN-----------EALIIESVKLVKKEIGSFTLFHQGVVVHDLPKTKTGKILRKTLYNMLN--GQDWKTPATVENPKVFEEIYSIL 1634
            + K +IT + G    +T+N K+IVDE +N         +V+ R  T+   M   RD  W  E  K   Y PPV+ ++ DP+F++YTSG+ G PKG+V T GG+++      +YV  ++  D  +  GD GWI+G T   +GPL +G ++I++E    S+  +   G   RII ++  T F  APT  R +K     +  K   + LR +   GE  +  L EW         C + D   QTE G     P  G       +   P   ++  +++  TG +L  N+  G + +K P  PS A S++N HDR++ TY   Y G+Y T D     +DG   I  R DDV++VS  R+    +E     +E+++ AA V + D + GQ   A++  K     NN           + L  E +  V+ EIG F      ++V DLP+T++GKI+R+ L  + +   +      T+ NP+V   I S +
Sbjct:  194 NSKVVITCDEGKRGGKTINTKKIVDEGLNGVDLV-SRILVFQRTGTEGIPMKAGRDYWWHEEAAKQRTYLPPVSCDAEDPLFLLYTSGSTGSPKGVVHTTGGYLLGAALTTRYVFDIHPEDVLFTAGDVGWITGHTYALYGPLTLGTASIIFE----STPAYPDYGRYWRIIQRHKATHFYVAPTALRLIKRVGEAEIAKYDTSSLRVLGSVGEPISPDLWEWYHEKVGNKNCVICDTMWQTESGSHLIAPLAGAVPTKPGSATVPFFGINACIIDPVTGVELEGNDVEGVLAVKSP-WPSMARSVWNHHDRYMDTYLKPYPGHYFTGDGAGRDHDGYYWIRGRVDDVVNVSGHRLSTSEIEASISNHENVSEAAVVGIPDELTGQTVVAYVSLKDGYLQNNATEGDAEHITPDNLRRELILQVRGEIGPFASPKTIILVRDLPRTRSGKIMRRVLRKVASNEAEQLGDLTTLANPEVVPAIISAV 673

HSP 2 Score: 51.2174 bits (121), Expect = 1.274e-63
Identity = 26/76 (34.21%), Postives = 45/76 (59.21%), Query Frame = 2 
Query:   26 VSYKQLLTLVENLAGVMVNKCHVQKGDRVLLYVPVTVQGIAAILACTRIGAVYVVTFGGFAPDLIAARIKHTEPKI 253
            +++ +LL  V  +AGV+     V+KGD V +Y+P+  + + A+LA  RIGA++ V F GF+   +  R+     K+
Sbjct:  123 ITFGELLRKVSQIAGVL-KSWGVKKGDTVAIYLPMIPEAVIAMLAVARIGAIHSVVFAGFSAGSLKDRVVDANSKV 197
[Close]
BLAST of Propionyl-CoA synthetase vs. Ensembl Nematostella
Match: EDO49293 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RH51])

HSP 1 Score: 279.641 bits (714), Expect = 2.779e-93
Identity = 160/473 (33.83%), Postives = 257/473 (54.33%), Query Frame = 3 
Query:  249 KFIITTNFGYEPERTVNYKEIVDEAINKSQFCPDYCIVYNRPNTKCATMIDSRDLDWETEIGKATPYPPVALESGDPIFIIYTSGTLGEPKGLVMTVGGFVVSNKYFMKYVLGLNRRDCWWMFGDYGWISGQTTMTFGPLLIGCSTILYEGKELSSHHFTGIGRIIDQNNVTGFSAAPTYFRFLKNKDSEKDI--KPSKAQLRFVCIGGELSNTKLKEWVSNVFLCPVYDLWGQTELGIIATGPGLGFKNISSNGLSPMLSVHP----KVVNSATGEQLPNETIGRMLLKIPLCPSAASSMYNDHDRFLKTYFSKXXXXXXXXXXXXIMNDGTLQILARADDVISVSAGRICGGALEEVCLMNEDLANAAFVALEDPINGQVPFAFLEKKKDRNINNEALIIESVKLVKKEIGSFTLFHQGVVVHDLPKTKTGKILRKTLYNMLNGQDWKTPATVENPKVFEEIYSILIEAGY 1649
            + I+T + G EP R V YK +VDEAI  S F P   ++Y R +     +I  RD+ W+  + +A P+  V + + DP++I+YTSGT G+PKG+V   GG  V+  + MK + G+N  + WW   D GW+ G + + + PL  GC+T+L+EGK + +       R+I+Q+ V     APT  R ++ +D + ++  +   +  R + + GE  +    +W       PVYD W QTE G   T   +G        L       P    K++     E    E +G++ +K+P+ P    +++   +RF KTYF KY GYYD+ D G++  DG + I+AR DDVI+V+  RI   +LEE  +    + +AA + L+D I G VP  F+   K  ++  E +I E  K V++ +G    F   V+V  LPKT++GK +R  L  +++G+ +K   T++NP+  ++I  +LI  GY
Sbjct:  212 RVIVTASCGIEPSRIVTYKPLVDEAIKISSFKPSTVVLYQR-DQCTGDIIPGRDITWDQVMERAEPHDCVPVLATDPLYILYTSGTTGDPKGIVRRNGGHAVALNWSMKNIYGVNPGEVWWAASDLGWVVGHSYIVYAPLFNGCTTVLFEGKPVGTPDAGAFFRVIEQHKVISMFTAPTAIRIIRTEDPKAELIRQYDLSHFRDMFLAGEHLDKDTMQWARRAISAPVYDNWWQTESGWAITAHTVGLGRPMDEKLETTGKAVPGYNVKILREDMTEADRGE-LGQICVKLPMPPGTMGTLWRADERFKKTYFEKYPGYYDSSDAGVMDEDGYVSIMARTDDVINVAGHRISTKSLEEGMMKPSFVVDAACIGLKDGIKGHVPLGFVIVDKTVSMKKEDMIKEVKKSVREFVGPVAAFKTAVIVPALPKTRSGKTVRGILAKIVHGEPYKVTPTMDNPQCLDQIKDVLISEGY 682

HSP 2 Score: 81.6481 bits (200), Expect = 2.779e-93
Identity = 40/84 (47.62%), Postives = 55/84 (65.48%), Query Frame = 2 
Query:    2 PVINATEKVSYKQLLTLVENLAGVMVNKCHVQKGDRVLLYVPVTVQGIAAILACTRIGAVYVVTFGGFAPDLIAARIKHTEPKI 253
            PV    E  SY+ L  LV   AG + N  +V KGDRV++Y+P+  Q + A+LAC RIGA++ V FGGFA   +A RI+H EP++
Sbjct:  131 PVSGKIEMYSYQNLQRLVAKFAGAL-NSLNVGKGDRVVIYMPMVPQAVIAMLACARIGAIHSVVFGGFAAAELARRIEHAEPRV 213
[Close]
BLAST of Propionyl-CoA synthetase vs. Ensembl Nematostella
Match: EDO39135 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SAZ3])

HSP 1 Score: 193.741 bits (491), Expect = 7.015e-64
Identity = 142/477 (29.77%), Postives = 228/477 (47.80%), Query Frame = 3 
Query:  228 ELNIQSQKFIITTNFGYEPERTVNYKEIVDEAINKSQFCPDYCIVYNRPNTKCATMIDSRDLDWETEIG-KATPYPPVALESGDPIFIIYTSGTLGEPKGLVMTVGGFVVSNKYFMKYVLGLNRRDCWWMFGDYGWISGQTTMTFGPLLIGCSTILYEGKELSSHHFTGIGR---IIDQNNVTGFSAAPTYFRFLKNKDSEKDIKPSKAQLRFVCIGGELSNTKLKEWVSNVF---LCPVYDLWGQTELGIIATGPGLGFKNISSN---GLSPMLSVHPKVVNSATGEQLPNETIGRMLLKIPLCPSAASSMYNDHDRFLKTYFSKXXXXXXXXXXXXIMNDGTLQILARADDVISVSAGRICGGALEEVCLMNEDLANAAFVALEDPINGQVPFAFLEKKKDRNINNEALIIESVKLVKKEIGSFTLFHQGVVVHDLPKTKTGKILRKTLYNMLNGQ--DWKTPATVENPKVFEEI 1622
             +N    + +IT N      R ++ K +VD+A++KS   P    V+    T     +   D+  E  +  +    PP  ++S DP+F++YTSG+ G+PKG+V T  G+++  K   KY     + D +    D GWI+G + + +GPL  G +T+L+E    S+  +   GR   ++++  +  F  APT  R L    +    K  ++ L+ +   GE  N +   W ++V     C V D W QTE G I   P     N        + P   V P +V+    E   N   G +  +  + P    ++Y  H+RFL TY+  Y GYY T D  +   DG  +I  R DDV++VS  R+    +E+    +  +A  A V    PI G+  +A+   K D  I  E    E  KLV+++IG+F +     VV  LPKT++GKI+R+ L  +   +  +    +T+ +P V E I
Sbjct:  117 RINDAQAETVITANQAVRGGRAIDLKRVVDDAVSKS---PGVKRVFVMKRTGAQVPMGKLDIPLEEAMAEQPDECPPEPMDSEDPLFLLYTSGSTGQPKGIVHTQAGYLLYAKLTHKYCFDYKQGDVFGCVADIGWITGHSYVVYGPLANGATTVLFE----STPTYPDPGRYWEMVERLRINQFYGAPTAIRLLLKSGNSWVEKYDRSSLKVLGTVGEPINEEAWHWYNDVVGEKRCTVVDTWWQTETGGILISPRPAPDNSPVKPEFPMRPFFGVDPVLVDEKGKELTGNNVTGALCFRSSV-PGMCRTIYGHHERFLNTYYRPYPGYYFTGDGALRDKDGHYRITGRMDDVVNVSGKRLGTAEVEDAMDNHSAVAETAVVGFPHPIKGEGIYAYATLKDDAQIEPEQFREEVKKLVRQKIGAFAVPEIIQVVPGLPKTRSGKIMRRILRKVAQDKPDELGDVSTLADPSVVEVI 585

HSP 2 Score: 69.707 bits (169), Expect = 7.015e-64
Identity = 37/76 (48.68%), Postives = 51/76 (67.11%), Query Frame = 2 
Query:   20 EKVSYKQLLTLVENLAGVMVNKCH-VQKGDRVLLYVPVTVQGIAAILACTRIGAVYVVTFGGFAPDLIAARIKHTE 244
            E VSY QL  +V  +A ++  KCH V+KGD V LY+PV+   +AA+LAC RIGA + V F GF+ D +A+RI   +
Sbjct:   49 EHVSYIQLYGMVNRMANML--KCHGVKKGDIVCLYMPVSPLAVAAMLACARIGAPHSVVFAGFSADALASRINDAQ 122
[Close]
BLAST of Propionyl-CoA synthetase vs. Ensembl Nematostella
Match: EDO42084 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S2B4])

HSP 1 Score: 162.155 bits (409), Expect = 3.917e-53
Identity = 136/492 (27.64%), Postives = 228/492 (46.34%), Query Frame = 3 
Query:  249 KFIITTNFGYEPERTVNYKEIVDEAINKSQ---FCPDYCIV-------------------YNRPNTKCATMIDSR-DLDWETEIGKAT-PYPPVALESGDPIFIIYTSGTLGEPKGLVMTVGGFVVSNKYFMKYVLGLNRRDCWWMFGDYGWISGQTTMTFGPLLIGCSTILYEGKELSSHHFTGIGR---IIDQNNVTGFSAAPTYFRFLKNKDSEKDIKPSKAQLRFVCIGGELSNTKLKEWVSNVF---LCPVYDLWGQTELG--IIATGPGLGFKNISSNGLSPMLSVHPKVVNSATGEQLPNETIGRMLLKIPLCPSAASSMYNDHDRFLKTYFSKXXXXXXXXXXXXIMNDGTLQILARADDVISVSAGRICGGALEEVCLMNEDLANAAFVALEDPINGQVPFAFLEKKKDRNINNEALIIESVKLVKKEIGSFTLFHQGVVVHDLPKTKTGKILRKTLYNML-NGQDWKTPATVENPKVFEEIY 1625
            K +IT +  Y   + +N KEI D AI K +   F  + CIV                     RP  +  T  DS  D  W   + +A+  +  V +++ DP+F++YTSG+ G+PKG++ T GG+++      +Y    +  + +W   D GWI+G + +T+GP+  G  ++L+EG       +   GR   I+D+  V+ F  APT  R L     E     +   L+ +   GE  N +   W  +V     C + D + QTE G  +I   PG   +        P   V P +V+   G +L     G +++K P  P  A ++Y +H RF   YF K+ GYY T D      +G   +  R DD ++VS   +    +E   + ++ ++ AA V+      G+  + F    ++  + +E LI E  + V+ +IG F           LPKT++GKI+R+ L  +  N +     +T+ +P V + ++
Sbjct:  196 KILITADASYRGLKMINLKEIADHAIIKCRERSFNVEACIVVRHLGPDKRVPTDPSSSPPPKRPCHQLQTSFDSSIDCWWHEVMAEASDEHDTVWVDAEDPLFMLYTSGSTGKPKGVLHTHGGYMLYTALTFRYSFDYHPGEVYWCTADIGWITGHSYITYGPMANGAISVLFEGTPF----YPDCGRFWDIVDKYKVSKFYTAPTAIRSLMKFGKEIVTSHNLESLKVLGTVGEPINPEAWLWYYHVVGHNKCAIVDTYWQTETGGHVITPLPG-ATRTRPGAATFPFFGVEPAIVDE-KGNELEGPCEGFLVIKRPW-PGMARTIYGNHKRFENVYFDKFRGYYTTGDGCKRDENGYYWVTGRTDDCMNVSGHLLSTAQIESALVEHDAVSEAAVVSCPHKEKGEGIYCFATLMENVEL-DEKLIRELKQKVRLKIGPFASPDVIQSAPALPKTRSGKIMRRVLRQVAKNDRQTGDLSTIYDPAVVDSLF 679

HSP 2 Score: 65.0846 bits (157), Expect = 3.917e-53
Identity = 33/79 (41.77%), Postives = 55/79 (69.62%), Query Frame = 2 
Query:   17 TEKVSYKQLLTLVENLAGVMVNKCHVQKGDRVLLYVPVTVQGIAAILACTRIGAVYVVTFGGFAPDLIAARIKHTEPKI 253
            T++++Y +LL  V   A V+ +K  V+KGDRV +Y+P+ ++ + A+LAC R+GAV+ + FGGF+ + +A RI  +  KI
Sbjct:  120 TKQITYGELLKQVCKFANVLESKG-VKKGDRVAIYMPMIMELVIAMLACARLGAVHSIVFGGFSAESLAERILDSTCKI 197
[Close]
BLAST of Propionyl-CoA synthetase vs. Ensembl Nematostella
Match: EDO44212 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RW67])

HSP 1 Score: 64.3142 bits (155), Expect = 4.836e-11
Identity = 95/376 (25.27%), Postives = 151/376 (40.16%), Query Frame = 3 
Query:  306 EIVDEAINKSQFCPDYCIV-YNRPNTKCATMIDSRDLDWETEIGKATPYPPVALESGDPIFIIY-TSGTLGEPKGLVMTVGGFVVSNKYFMKYVLGLNRRDCWWMFGDYGWISGQTTMTFGPLLIGCSTIL------YEGKELSSHHFTGIGRIIDQNNVTGFSAAPTYFRFLKNKDSEKDIKPSKAQLRFVCIGGELSNTK-LKEWVSNVFLCPVYDLWGQTELGIIATGPGLGFKNISSNGLS---PMLSVHPKVVNSATGEQLPNETIGRMLLKI-PLCPSAASSMY-NDHDRFLKTYFSKXXXXXXXXXXXXIMNDGTLQILARADDVISVSAGRICGGALEEVCLMNEDLANAAFVALEDPINGQVPFAFL 1391
            E+VDE  NK  F     IV ++R             L ++T   +A+     A  + D   II+ TSGT G PK    T     + +    KY L L   D  W   D GW        +GP + G S  +      +E K +  H        + +  ++     PT +R + ++D  +   P+   LR     GE  N + + +W     L  + + +GQTE  ++       F+ I +   S   P      +V++    E  P    G + + + P  P    S Y  D++R +  Y   Y    D         DG L  + R+DDVI  +  RI    +E   + +  +A AA V+  DP+ G+V  AF+
Sbjct:   56 EVVDEVYNKINFLSFNLIVSFHRKGW----------LPFKTLYKQASTVHTCAPTTSDETSIIFFTSGTTGLPKMTQHTHASQGIGHSITGKYWLDLCPDDVHWTVSDPGWAKTAYGCLYGPWIQGASVFIHHTTEKFEAKVILDH--------LQKYPISTMCLPPTAYRMMIHEDLSRYQFPA---LRHCLSAGEPLNPEVMVDWKEKTGL-DIREGYGQTETTLLCG----TFRCIETRPGSMGKPAPGYDVRVIDEKCNET-PAGIEGDIAVYLKPRRPVGLFSEYIEDYERNVSAYRGDYYLTGDRAVRD---EDGYLWFIGRSDDVIISAGYRIGPFEVESALIEHPAVAEAAVVSSPDPVRGEVVKAFV 401
[Close]
BLAST of Propionyl-CoA synthetase vs. Ensembl Nematostella
Match: EDO37992 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SE80])

HSP 1 Score: 56.9954 bits (136), Expect = 1.596e-8
Identity = 93/412 (22.57%), Postives = 168/412 (40.78%), Query Frame = 3 
Query:  366 NRPNTKCATMIDSRDLDWETEIGKATPYPPVALESGDPIFIIYTSGTLGEPKGLVMTVGGFVVSNKYFMKYVLGLNRRDCWWMFGDYGWISGQTTMTFGPLLIGCSTILYEGKELSSHHFTGIG-RIIDQNNVTGFSAAPTYFRFLKNKDSEKDIKPSKAQLRFVCIGGELSNTKLKEWVSNVF--LCPVYDLWGQTEL----GIIATGPGLGFKNISSNGLSPMLSVHPKVVNSATGEQLPNETIGRMLLKIPLCPSAASSMYNDHDRFLKTYFSKXXXX--XXXXXXXXIMNDGTLQILARADDVISVSAGRICGGALEEVCLMNEDLANAAFVALEDPINGQVPFAFLEKKKDRNINNEALIIE-----SVKLVKKEIGSFTLFHQGVVVHDLPKTKTGKILRKTLYNM 1559
            N PN K     D+    ++ +           ++  DP  +I+TSG+ G PK ++ T  GFV +    + +          +    + WI G         ++G + + +        H  GI  +II +   T   A    +  L     E   +   +QL+    GG+L++  L   V +    L  + + +G TE     G + T   +   +  +  ++P   V  KVV+   G  +P  T G + ++      +A+ +     R     + +      Y + D   I NDG ++IL R D +I  +   I    +E V   +E +A+   + + D   G+V  A +  K   + +N+A+I E     + K      G      + V++ + PKT+TGK+ RK L N+
Sbjct:  153 NMPNHKKVHSYDALLQSFQDDDITQLHQAETEVQFDDPALVIFTSGSTGRPKPILYTHHGFV-NGALSVVHTFKATHDTIQFCDAPFDWIPGIGFSLALVSILGMTLVAFPPNLSIKGHVIGIMLKIISEERCT--HAIMLTYVMLDMVRYEGLPQLDLSQLKVCITGGQLTDQHLMSKVFSALPDLTSIVNSYGSTETFLPSGQVVTRHNIHSLDYGATEVNPGFEV--KVVDDE-GHVVPVGTPGELHVRGAGILQSANGV-----RMEGVVYEEKTPTGWYPSKDLSKITNDGRVRILGRKDCLIKSATESIYPPEVESVLGKHEKIASIIAIGIPDQRLGEVVCACVIIKPGFHGDNKAVIQEIDEWCAPKFYVSSNGMTPQPKKYVILENFPKTRTGKVDRKALKNI 553
[Close]
BLAST of Propionyl-CoA synthetase vs. Ensembl Medaka
Match: acss3 (acyl-CoA synthetase short chain family member 3 [Source:NCBI gene;Acc:101161021])

HSP 1 Score: 290.812 bits (743), Expect = 1.337e-92
Identity = 170/464 (36.64%), Postives = 260/464 (56.03%), Query Frame = 3 
Query:  249 KFIITTNFGYEPERTVNYKEIVDEAINKSQFCPDYCIVYNRPNTKCATMIDSRDLDWETEIGKATPYPPVALESGDPIFIIYTSGTLGEPKGLVMTVGGFVVSNKYFMKYVLGLNRRDCWWMFGDYGWISGQTTMTFGPLLIGCSTILYEGKELSSHHFTGIGRIIDQNNVTGFSAAPTYFRFLKNKD--SEKDIKPSKAQLRFVCIGGELSNTKLKEWVSNVFLCPVYDLWGQTELG--IIATGPGLGFKNISSNGLS--PMLSVHPKVVNSATGEQLPNETIGRMLLKIPLCPSAASSMYNDHDRFLKTYFSKXXXXXXXXXXXXIMNDGTLQILARADDVISVSAGRICGGALEEVCLMNEDLANAAFVALEDPINGQVPFAFLEKKKDRNINNEALIIESVKLVKKEIGSFTLFHQGVVVHDLPKTKTGKILRKTLYNMLNGQDWKTPATVENPKVFEEI 1622
            K ++T +FG EP R V Y  +V +A+  S   P   ++YNRPN +   +     LDW+ E+  A P+  V+L S  P++++YTSGT G PKG+V    G+ V  K+ M  + GL   D WW   D GW+ G + + + PLL G STILYEGK + +       R++ ++  +    APT  R ++ +D  +    K +  +L+ + + GE  + +  EW    F  PV D W QTE G  I A+  GLG       G +  P+   +  V++    E  P  T+G +++++PL P AA S++ +   F K YFSK+ GYYDTMD G +  DG L I++R+DDVI+V+  R+  GALEE  L++  + + A V LED + G +P A    K +     E +  E VKLV+  +G    F   + V  LPKT++GKI R +L N++NG+ +K   T+E+P+VF++I
Sbjct:  235 KLMVTASFGVEPGRRVEYIPLVKKALEMSCHRPSKVLIYNRPNMEKVFLSPELSLDWDEEMASARPHECVSLPSNHPLYVLYTSGTTGTPKGVVRDTAGYAVMLKWTMSNIYGLKPGDIWWAASDLGWVVGHSYICYAPLLHGNSTILYEGKPVGTPDPGAFFRVLSEHGASSMFTAPTAIRAIRQQDPHAANGKKYALNRLQSLFVAGERCDVETLEWAKKNFGVPVLDHWWQTETGSAITASFMGLGGSLTPPAGQAGKPVPGYNVTVIDDQMQEVKP-RTLGNIVVRLPLPPGAALSLWENKSLFKKLYFSKFPGYYDTMDAGFLDEDGFLYIMSRSDDVINVAGHRLSAGALEESVLLHSAVGDCAVVGLEDSLKGHIPLALCVLKNEVRDTEEKVRTEIVKLVRDTVGPVAAFKNVLFVQGLPKTRSGKIPRSSLSNLINGKPYKVTPTIEDPEVFKDI 697

HSP 2 Score: 70.0922 bits (170), Expect = 1.337e-92
Identity = 35/84 (41.67%), Postives = 55/84 (65.48%), Query Frame = 2 
Query:    2 PVINATEKVSYKQLLTLVENLAGVMVNKCHVQKGDRVLLYVPVTVQGIAAILACTRIGAVYVVTFGGFAPDLIAARIKHTEPKI 253
            PV    + ++Y +L   V  LAGV+V K  V++GD V++Y+P+  + + A+LAC RIGA + + FGGFA   ++ RI H +PK+
Sbjct:  154 PVTGTKKIITYGELQAQVSKLAGVLV-KNGVRQGDLVVIYMPMVPEAMIAMLACARIGAPHSLIFGGFASKELSVRIDHAKPKL 236
[Close]
BLAST of Propionyl-CoA synthetase vs. Ensembl Medaka
Match: acss3 (acyl-CoA synthetase short chain family member 3 [Source:NCBI gene;Acc:101161021])

HSP 1 Score: 291.197 bits (744), Expect = 3.485e-89
Identity = 170/472 (36.02%), Postives = 263/472 (55.72%), Query Frame = 3 
Query:  225 LELNIQSQKFIITTNFGYEPERTVNYKEIVDEAINKSQFCPDYCIVYNRPNTKCATMIDSRDLDWETEIGKATPYPPVALESGDPIFIIYTSGTLGEPKGLVMTVGGFVVSNKYFMKYVLGLNRRDCWWMFGDYGWISGQTTMTFGPLLIGCSTILYEGKELSSHHFTGIGRIIDQNNVTGFSAAPTYFRFLKNKD--SEKDIKPSKAQLRFVCIGGELSNTKLKEWVSNVFLCPVYDLWGQTELG--IIATGPGLGFKNISSNGLS--PMLSVHPKVVNSATGEQLPNETIGRMLLKIPLCPSAASSMYNDHDRFLKTYFSKXXXXXXXXXXXXIMNDGTLQILARADDVISVSAGRICGGALEEVCLMNEDLANAAFVALEDPINGQVPFAFLEKKKDRNINNEALIIESVKLVKKEIGSFTLFHQGVVVHDLPKTKTGKILRKTLYNMLNGQDWKTPATVENPKVFEEI 1622
            + ++    K ++T +FG EP R V Y  +V +A+  S   P   ++YNRPN +   +     LDW+ E+  A P+  V+L S  P++++YTSGT G PKG+V    G+ V  K+ M  + GL   D WW   D GW+ G + + + PLL G STILYEGK + +       R++ ++  +    APT  R ++ +D  +    K +  +L+ + + GE  + +  EW    F  PV D W QTE G  I A+  GLG       G +  P+   +  V++    E  P  T+G +++++PL P AA S++ +   F K YFSK+ GYYDTMD G +  DG L I++R+DDVI+V+  R+  GALEE  L++  + + A V LED + G +P A    K +     E +  E VKLV+  +G    F   + V  LPKT++GKI R +L N++NG+ +K   T+E+P+VF++I
Sbjct:  202 VRIDHAKPKLMVTASFGVEPGRRVEYIPLVKKALEMSCHRPSKVLIYNRPNMEKVFLSPELSLDWDEEMASARPHECVSLPSNHPLYVLYTSGTTGTPKGVVRDTAGYAVMLKWTMSNIYGLKPGDIWWAASDLGWVVGHSYICYAPLLHGNSTILYEGKPVGTPDPGAFFRVLSEHGASSMFTAPTAIRAIRQQDPHAANGKKYALNRLQSLFVAGERCDVETLEWAKKNFGVPVLDHWWQTETGSAITASFMGLGGSLTPPAGQAGKPVPGYNVTVIDDQMQEVKP-RTLGNIVVRLPLPPGAALSLWENKSLFKKLYFSKFPGYYDTMDAGFLDEDGFLYIMSRSDDVINVAGHRLSAGALEESVLLHSAVGDCAVVGLEDSLKGHIPLALCVLKNEVRDTEEKVRTEIVKLVRDTVGPVAAFKNVLFVQGLPKTRSGKIPRSSLSNLINGKPYKVTPTIEDPEVFKDI 672

HSP 2 Score: 70.0922 bits (170), Expect = 3.709e-12
Identity = 35/84 (41.67%), Postives = 55/84 (65.48%), Query Frame = 2 
Query:    2 PVINATEKVSYKQLLTLVENLAGVMVNKCHVQKGDRVLLYVPVTVQGIAAILACTRIGAVYVVTFGGFAPDLIAARIKHTEPKI 253
            PV    + ++Y +L   V  LAGV+V K  V++GD V++Y+P+  + + A+LAC RIGA + + FGGFA   ++ RI H +PK+
Sbjct:  129 PVTGTKKIITYGELQAQVSKLAGVLV-KNGVRQGDLVVIYMPMVPEAMIAMLACARIGAPHSLIFGGFASKELSVRIDHAKPKL 211
[Close]
BLAST of Propionyl-CoA synthetase vs. Ensembl Medaka
Match: acss3 (acyl-CoA synthetase short chain family member 3 [Source:NCBI gene;Acc:101161021])

HSP 1 Score: 261.536 bits (667), Expect = 7.723e-84
Identity = 161/464 (34.70%), Postives = 247/464 (53.23%), Query Frame = 3 
Query:  249 KFIITTNFGYEPERTVNYKEIVDEAINKSQFCPDYCIVYNRPNTKCATMIDSRDLDWETEIGKATPYPPVALESGDPIFIIYTSGTLGEPKGLVMTVGGFVVSNKYFMKYVLGLNRRDCWWMFGDYGWISGQTTMTFGPLLIGCSTILYEGKELSSHHFTGIGRIIDQNNVTGFSAAPTYFRFLKNKD--SEKDIKPSKAQLRFVCIGGELSNTKLKEWVSNVFLCPVYDLWGQTELG--IIATGPGLGFKNISSNGLS--PMLSVHPKVVNSATGEQLPNETIGRMLLKIPLCPSAASSMYNDHDRFLKTYFSKXXXXXXXXXXXXIMNDGTLQILARADDVISVSAGRICGGALEEVCLMNEDLANAAFVALEDPINGQVPFAFLEKKKDRNINNEALIIESVKLVKKEIGSFTLFHQGVVVHDLPKTKTGKILRKTLYNMLNGQDWKTPATVENPKVFEEI 1622
            K ++T +FG EP R V Y  +V +A+  S   P   ++YNRPN +   +     LDW+ E+  A P+  V+L S  P++++YTSGT G PK                            WW   D GW+ G + + + PLL G STILYEGK + +       R++ ++  +    APT  R ++ +D  +    K +  +L+ + + GE  + +  EW    F  PV D W QTE G  I A+  GLG       G +  P+   +  V++    E  P  T+G +++++PL P AA S++ +   F K YFSK+ GYYDTMD G +  DG L I++R+DDVI+V+  R+  GALEE  L++  + + A V LED + G +P A    K +     E +  E VKLV+  +G    F   + V  LPKT++GKI R +L N++NG+ +K   T+E+P+VF++I
Sbjct:  235 KLMVTASFGVEPGRRVEYIPLVKKALEMSCHRPSKVLIYNRPNMEKVFLSPELSLDWDEEMASARPHECVSLPSNHPLYVLYTSGTTGTPK---------------------------IWWAASDLGWVVGHSYICYAPLLHGNSTILYEGKPVGTPDPGAFFRVLSEHGASSMFTAPTAIRAIRQQDPHAANGKKYALNRLQSLFVAGERCDVETLEWAKKNFGVPVLDHWWQTETGSAITASFMGLGGSLTPPAGQAGKPVPGYNVTVIDDQMQEVKP-RTLGNIVVRLPLPPGAALSLWENKSLFKKLYFSKFPGYYDTMDAGFLDEDGFLYIMSRSDDVINVAGHRLSAGALEESVLLHSAVGDCAVVGLEDSLKGHIPLALCVLKNEVRDTEEKVRTEIVKLVRDTVGPVAAFKNVLFVQGLPKTRSGKIPRSSLSNLINGKPYKVTPTIEDPEVFKDI 670

HSP 2 Score: 70.0922 bits (170), Expect = 7.723e-84
Identity = 35/84 (41.67%), Postives = 55/84 (65.48%), Query Frame = 2 
Query:    2 PVINATEKVSYKQLLTLVENLAGVMVNKCHVQKGDRVLLYVPVTVQGIAAILACTRIGAVYVVTFGGFAPDLIAARIKHTEPKI 253
            PV    + ++Y +L   V  LAGV+V K  V++GD V++Y+P+  + + A+LAC RIGA + + FGGFA   ++ RI H +PK+
Sbjct:  154 PVTGTKKIITYGELQAQVSKLAGVLV-KNGVRQGDLVVIYMPMVPEAMIAMLACARIGAPHSLIFGGFASKELSVRIDHAKPKL 236
[Close]
BLAST of Propionyl-CoA synthetase vs. Ensembl Medaka
Match: acss3 (acyl-CoA synthetase short chain family member 3 [Source:NCBI gene;Acc:101161021])

HSP 1 Score: 259.61 bits (662), Expect = 1.311e-77
Identity = 165/471 (35.03%), Postives = 250/471 (53.08%), Query Frame = 3 
Query:  225 LELNIQSQKFIITTNFGYEPERTVNYKEIVDEAINKSQFCPDYCIVYNRPNTKCATMIDSRDLDWETEIGKATPYPPVALESGDPIFIIYTSGTLGEPKGLVMTVGGFVVSNKYFMKYVLGLNRRDCWWMFGDYGWISGQTTMTFGPLLIGCSTILYEGKELSSHHFTGIGRIIDQNNVTGFSAAPTYFRFLKNKD--SEKDIKPSKAQLRFVCIGGELSNTKLKEWVSNVFLCPVYDLWGQTELG--IIATGPGLGFKNISSNGLSPMLSVHPKVVNSATGEQLPNETIGRMLLKIPLCPSAASSMYNDHDRFLKTYFSKXXXXXXXXXXXXIMNDGTLQILARADDVISVSAGRICGGALEEVCLMNEDLANAAF-VALEDPINGQVPFAFLEKKKDRNINNEALIIESVKLVKKEIGSFTLFHQGVVVHDLPKTKTGKILRKTLYNMLNGQDWKTPATVENPKVFEEI 1622
            + ++    K ++T +FG EP R V Y  +V +A+  S   P   ++YNRPN +   +     LDW+ E+  A P+  V+L S  P++++YTSGT G PKG+V    G+ V  K+ M  + GL   D WW   D GW+ G + + + PLL G STILYEGK + +       R++ ++  +    APT  R ++ +D  +    K +  +L+ + + GE  + +  EW    F  PV D W QTE G  I A+  GLG       G +        V++    E  P  T+G +++++PL P AA S++ +   F K YFSK+ GYYDTMD G +  DG L I++R+DDVI+V+  R+  GALEEV        N AF V + D                     E +  E VKLV+  +G    F   + V  LPKT++GKI R +L N++NG+ +K   T+E+P+VF++I
Sbjct:  200 VRIDHAKPKLMVTASFGVEPGRRVEYIPLVKKALEMSCHRPSKVLIYNRPNMEKVFLSPELSLDWDEEMASARPHECVSLPSNHPLYVLYTSGTTGTPKGVVRDTAGYAVMLKWTMSNIYGLKPGDIWWAASDLGWVVGHSYICYAPLLHGNSTILYEGKPVGTPDPGAFFRVLSEHGASSMFTAPTAIRAIRQQDPHAANGKKYALNRLQSLFVAGERCDVETLEWAKKNFGVPVLDHWWQTETGSAITASFMGLGGSLTPPAGQAGKPVPGYNVIDDQMQEVKP-RTLGNIVVRLPLPPGAALSLWENKSLFKKLYFSKFPGYYDTMDAGFLDEDGFLYIMSRSDDVINVAGHRLSAGALEEV-------RNPAFAVCVRD-------------------TEEKVRTEIVKLVRDTVGPVAAFKNVLFVQGLPKTRSGKIPRSSLSNLINGKPYKVTPTIEDPEVFKDI 643

HSP 2 Score: 65.855 bits (159), Expect = 7.929e-11
Identity = 35/84 (41.67%), Postives = 55/84 (65.48%), Query Frame = 2 
Query:    2 PVINATEKVSYKQLLTLVENLAGVMVNKCHVQKGDRVLLYVPVTVQGIAAILACTRIGAVYVVTFGGFAPDLIAARIKHTEPKI 253
            PV    + ++Y +L   V  LAGV+V K  V++GD V++Y+P+  + + A+LAC RIGA + + FGGFA   ++ RI H +PK+
Sbjct:  129 PVTGTKKIITYGELQ--VSKLAGVLV-KNGVRQGDLVVIYMPMVPEAMIAMLACARIGAPHSLIFGGFASKELSVRIDHAKPKL 209
[Close]
BLAST of Propionyl-CoA synthetase vs. Ensembl Medaka
Match: ENSORLT00000021863.2 (acyl-CoA synthetase short chain family member 1 [Source:NCBI gene;Acc:101167010])

HSP 1 Score: 185.267 bits (469), Expect = 1.940e-57
Identity = 143/482 (29.67%), Postives = 230/482 (47.72%), Query Frame = 3 
Query:  252 FIITTNFGYEPERTVNYKEIVDEAINKSQFCPDYCIVYNRPNTKCATMIDSRDLDWETEIGKATPY-PPVALESGDPIFIIYTSGTLGEPKGLVMTVGGFVVSNKYFMKYVLGLNRRDCWWMFGDYGWISGQTTMTFGPLLIGCSTILYEGKELSSHHFTGIGR---IIDQNNVTGFSAAPTYFRFLKNKDSEKDIKPSKAQLRFVCIGGELSNTKLKEWVSNVF---LCPVYDLWGQTELGIIATGPGLGFKN---ISSNGLSPMLSVHPKVVNSATGEQLPNETIGRMLLKIPLCPSAASSMYNDHDRFLKTYFSKXXXXXXXXXXXXIMNDGTLQILARADDVISVSAGRICGGALEEVCLMNEDLANAAFVALEDPINGQVPFAFLEKKKDRNINNEALIIESVKLVKKEIGSFTLFHQGVVVHDLPKTKTGKILRKTLYNMLNG--QDWKTPATVENPKVFEEIYSILIEAGYKASL 1661
             +IT N G    +    K+ VD A+   Q CP    V+    T+    +  RD+  + E+ K      P A+ S D +F++YTSG+ G+PKGLV T  G+++      +YV   +  D +    D GWI+G + + +GPL  G +T+L+E    S+  +   GR    +++  +  F  APT  R L     +   K  ++ L+ +   GE  NT+  EW   V     CPV D W QTE G I   P     +   +    + P   + P ++++       N T G + +  P  P  A +++N+H+RF++TY   Y GY+ T D       G  QI  R DDVI+VS  RI    +E+V      +A +A +     I GQ  +AF+  KK+  I  E L  +   +V ++I  +      ++V  LPKT++GKI+R+ L  ++          +T+++P   +EI    IE   K S+
Sbjct:  215 IVITMNQGVRGGKVTELKKTVDTAV---QSCPTVRHVFVAMRTETLVPMTDRDVLMDEEMLKEDVVCEPAAMNSEDVLFLLYTSGSTGKPKGLVHTQAGYLLYAALTQRYVFSYHDGDVFGCVADIGWITGHSYIVYGPLANGATTVLFE----STPVYPDPGRYWETVERLKINQFYGAPTAIRLLLKYGDQWVNKYDRSSLKTLGSVGEPINTEAWEWYHRVVGEGRCPVVDTWWQTETGGICIAPKPSEPDEEIVPGMAMRPFFGIKPVLMDTEGKVLTANNTSGALCIAQPW-PGMARTIHNNHERFIETYCQPYPGYFFTGDGAYRSPKGYYQITGRLDDVINVSGHRIGTAEIEDVVNQCSAVAESAVIGYSHDIKGQGIYAFVVLKKNMKIKEEDLYKQLNSVVSEKIAKYACPDFILLVKRLPKTRSGKIMRRVLRKVVESDLDSLGDLSTLDDPAAVQEI----IEGHSKISM 684

HSP 2 Score: 58.151 bits (139), Expect = 1.940e-57
Identity = 28/77 (36.36%), Postives = 48/77 (62.34%), Query Frame = 2 
Query:   23 KVSYKQLLTLVENLAGVMVNKCHVQKGDRVLLYVPVTVQGIAAILACTRIGAVYVVTFGGFAPDLIAARIKHTEPKI 253
            + +Y++LL +   + G ++ +  V+KGD V +Y+P     +A++LAC RIGA + V F GF+ D +A RI+  +  I
Sbjct:  140 RYTYRELLEMTCRV-GNLLKRHGVRKGDCVTIYMPTCPLAVASMLACARIGAAHNVVFAGFSADALAERIRDAQSSI 215
[Close]
BLAST of Propionyl-CoA synthetase vs. Planmine SMEST
Match: SMESG000043645.1 (SMESG000043645.1)

HSP 1 Score: 947.192 bits (2447), Expect = 0.000e+0
Identity = 470/479 (98.12%), Postives = 473/479 (98.75%), Query Frame = 3 
Query:  249 KFIITTNFGYEPERTVNYKEIVDEAINKSQFCPDYCIVYNRPNTKCATMIDSRDLDWETEIGKATPYPPVALESGDPIFIIYTSGTLGEPKGLVMTVGGFVVSNKYFMKYVLGLNRRDCWWMFGDYGWISGQTTMTFGPLLIGCSTILYEGKELSSHHFTGIGRIIDQNNVTGFSAAPTYFRFLKNKDSEKDIKPSKAQLRFVCIGGELSNTKLKEWVSNVFLCPVYDLWGQTELGIIATGPGLGFKNISSNGLSPMLSVHPKVVNSATGEQLPNETIGRMLLKIPLCPSAASSMYNDHDRFLKTYFSKXXXXXXXXXXXXIMNDGTLQILARADDVISVSAGRICGGALEEVCLMNEDLANAAFVALEDPINGQVPFAFLEKKKDRNINNEALIIESVKLVKKEIGSFTLFHQGVVVHDLPKTKTGKILRKTLYNMLNGQDWKTPATVENPKVFEEIYSILIEAGYKASLPNDNSLNM 1685
            K IITTNFGYEPERTVNYKEIVDEAINKSQFCPDYCIVYNRPNTKCATMIDSRDLDWETEIGKATPY PVALESGDPIFIIYTSGTLGEPKGLVMTVGGF+VSNKYFMKYVLGLNR+DCWWMFGDYGWISGQTTMTFGPLLIGCSTILYEGKELSSHH TGIG+IIDQNNVTGFSAAPTYFRFLKNKDSEKDIKPSKAQLRFVCIGGELSNTKLKEWVSNVFLCPVYDLWGQTELGIIATGPGLGFKNISSNGLSPMLSVHPKVVNSATGE LPNETIGRMLLKIPLCPSAASSMY DHDRFLKTYFSKYDGYYDTMDYGMI NDGTLQILARADDVISVSAGRICGGALEEVCLMNEDLANAAFVALEDPINGQVPFAFLEKKKDRNINNEALIIESVKLVKKEIGSFTLFHQGVVVHDLPKTKTGKILRKTLYNMLNGQDWKTPATVENPKVFEEIYSILIEAGYKASLPNDNSLNM
Sbjct:  107 KLIITTNFGYEPERTVNYKEIVDEAINKSQFCPDYCIVYNRPNTKCATMIDSRDLDWETEIGKATPYAPVALESGDPIFIIYTSGTLGEPKGLVMTVGGFIVSNKYFMKYVLGLNRQDCWWMFGDYGWISGQTTMTFGPLLIGCSTILYEGKELSSHHLTGIGKIIDQNNVTGFSAAPTYFRFLKNKDSEKDIKPSKAQLRFVCIGGELSNTKLKEWVSNVFLCPVYDLWGQTELGIIATGPGLGFKNISSNGLSPMLSVHPKVVNSATGEHLPNETIGRMLLKIPLCPSAASSMYKDHDRFLKTYFSKYDGYYDTMDYGMIKNDGTLQILARADDVISVSAGRICGGALEEVCLMNEDLANAAFVALEDPINGQVPFAFLEKKKDRNINNEALIIESVKLVKKEIGSFTLFHQGVVVHDLPKTKTGKILRKTLYNMLNGQDWKTPATVENPKVFEEIYSILIEAGYKASLPNDNSLNM 585

HSP 2 Score: 169.088 bits (427), Expect = 4.354e-45
Identity = 82/84 (97.62%), Postives = 84/84 (100.00%), Query Frame = 2 
Query:    2 PVINATEKVSYKQLLTLVENLAGVMVNKCHVQKGDRVLLYVPVTVQGIAAILACTRIGAVYVVTFGGFAPDLIAARIKHTEPKI 253
            PVINATEKVSYKQLLTLVENLAGVMVNKCH+QKGDRVLLYVPVTVQGIAAILACTRIGAVYVVTFGGFAPDLIAARIKHTEPK+
Sbjct:   25 PVINATEKVSYKQLLTLVENLAGVMVNKCHLQKGDRVLLYVPVTVQGIAAILACTRIGAVYVVTFGGFAPDLIAARIKHTEPKL 108
[Close]
BLAST of Propionyl-CoA synthetase vs. Planmine SMEST
Match: SMESG000043645.1 (SMESG000043645.1)

HSP 1 Score: 944.11 bits (2439), Expect = 0.000e+0
Identity = 470/479 (98.12%), Postives = 473/479 (98.75%), Query Frame = 3 
Query:  249 KFIITTNFGYEPERTVNYKEIVDEAINKSQFCPDYCIVYNRPNTKCATMIDSRDLDWETEIGKATPYPPVALESGDPIFIIYTSGTLGEPKGLVMTVGGFVVSNKYFMKYVLGLNRRDCWWMFGDYGWISGQTTMTFGPLLIGCSTILYEGKELSSHHFTGIGRIIDQNNVTGFSAAPTYFRFLKNKDSEKDIKPSKAQLRFVCIGGELSNTKLKEWVSNVFLCPVYDLWGQTELGIIATGPGLGFKNISSNGLSPMLSVHPKVVNSATGEQLPNETIGRMLLKIPLCPSAASSMYNDHDRFLKTYFSKXXXXXXXXXXXXIMNDGTLQILARADDVISVSAGRICGGALEEVCLMNEDLANAAFVALEDPINGQVPFAFLEKKKDRNINNEALIIESVKLVKKEIGSFTLFHQGVVVHDLPKTKTGKILRKTLYNMLNGQDWKTPATVENPKVFEEIYSILIEAGYKASLPNDNSLNM 1685
            K IITTNFGYEPERTVNYKEIVDEAINKSQFCPDYCIVYNRPNTKCATMIDSRDLDWETEIGKATPY PVALESGDPIFIIYTSGTLGEPKGLVMTVGGF+VSNKYFMKYVLGLNR+DCWWMFGDYGWISGQTTMTFGPLLIGCSTILYEGKELSSHH TGIG+IIDQNNVTGFSAAPTYFRFLKNKDSEKDIKPSKAQLRFVCIGGELSNTKLKEWVSNVFLCPVYDLWGQTELGIIATGPGLGFKNISSNGLSPMLSVHPKVVNSATGE LPNETIGRMLLKIPLCPSAASSMY DHDRFLKTYFSKYDGYYDTMDYGMI NDGTLQILARADDVISVSAGRICGGALEEVCLMNEDLANAAFVALEDPINGQVPFAFLEKKKDRNINNEALIIESVKLVKKEIGSFTLFHQGVVVHDLPKTKTGKILRKTLYNMLNGQDWKTPATVENPKVFEEIYSILIEAGYKASLPNDNSLNM
Sbjct:  162 KLIITTNFGYEPERTVNYKEIVDEAINKSQFCPDYCIVYNRPNTKCATMIDSRDLDWETEIGKATPYAPVALESGDPIFIIYTSGTLGEPKGLVMTVGGFIVSNKYFMKYVLGLNRQDCWWMFGDYGWISGQTTMTFGPLLIGCSTILYEGKELSSHHLTGIGKIIDQNNVTGFSAAPTYFRFLKNKDSEKDIKPSKAQLRFVCIGGELSNTKLKEWVSNVFLCPVYDLWGQTELGIIATGPGLGFKNISSNGLSPMLSVHPKVVNSATGEHLPNETIGRMLLKIPLCPSAASSMYKDHDRFLKTYFSKYDGYYDTMDYGMIKNDGTLQILARADDVISVSAGRICGGALEEVCLMNEDLANAAFVALEDPINGQVPFAFLEKKKDRNINNEALIIESVKLVKKEIGSFTLFHQGVVVHDLPKTKTGKILRKTLYNMLNGQDWKTPATVENPKVFEEIYSILIEAGYKASLPNDNSLNM 640

HSP 2 Score: 169.474 bits (428), Expect = 6.877e-45
Identity = 82/84 (97.62%), Postives = 84/84 (100.00%), Query Frame = 2 
Query:    2 PVINATEKVSYKQLLTLVENLAGVMVNKCHVQKGDRVLLYVPVTVQGIAAILACTRIGAVYVVTFGGFAPDLIAARIKHTEPKI 253
            PVINATEKVSYKQLLTLVENLAGVMVNKCH+QKGDRVLLYVPVTVQGIAAILACTRIGAVYVVTFGGFAPDLIAARIKHTEPK+
Sbjct:   80 PVINATEKVSYKQLLTLVENLAGVMVNKCHLQKGDRVLLYVPVTVQGIAAILACTRIGAVYVVTFGGFAPDLIAARIKHTEPKL 163
[Close]
BLAST of Propionyl-CoA synthetase vs. Planmine SMEST
Match: SMESG000043642.1 (SMESG000043642.1)

HSP 1 Score: 528.48 bits (1360), Expect = 0.000e+0
Identity = 254/472 (53.81%), Postives = 344/472 (72.88%), Query Frame = 3 
Query:  249 KFIITTNFGYEPERTVNYKEIVDEAINKSQFCPDYCIVYNRPNTKCATMIDSRDLDWETEIGKATPYPPVALESGDPIFIIYTSGTLGEPKGLVMTVGGFVVSNKYFMKYVLGLNRRDCWWMFGDYGWISGQTTMTFGPLLIGCSTILYEGKELSSHHFTGIGRIIDQNNVTGFSAAPTYFRFLKNKDSEKDIKPSKAQLRFVCIGGELSNTKLKEWVSNVFLCPVYDLWGQTELGIIATGPGLGFKNISSNGLSPMLSVHPKVVNSATGEQLPNETIGRMLLKIPLCPSAASSMYNDHDRFLKTYFSKXXXXXXXXXXXXIMNDGTLQILARADDVISVSAGRICGGALEEVCLMNEDLANAAFVALEDPINGQVPFAFLEKKKDRNINNEALIIESVKLVKKEIGSFTLFHQGVVVHDLPKTKTGKILRKTLYNMLNGQDWKTPATVENPKVFEEIYSILIEAGYKASLP 1664
            K +ITTN GYEP RT+ YKE V++AI  SQF PDYC+VYNR NT  A M++ +DLDWETEI  ATP+  V LES  P++IIYTSGT GEPK LVM  G  +V++KYF+KYV G+ R+DC+WMF D+GWISGQT MT+GPLL+GC+ ILYEGK L+    T + +II+++NV  F + PT+FR +K    E DIK    QL+ +CIGGE SN +L++W+S +F  P++++WGQ+ELG + TG   G+   + +   P  S++PK++NS T EQLPN T+GR++LK P+ PSAA ++Y +H ++L  +F+KY GY+DTMDYGM+ +DG+LQ+L+R++ +I +S+  ICGG +EEVCL NE L NAA V L+DP+ GQVP AFLE K D+ I N  +I   ++LV K++G F  F   V+V +LPK + GK+LR +L  M NG+ W  P  VE P VF+EIY +LI AGYKASLP
Sbjct:  162 KLVITTNIGYEPGRTIKYKEHVEDAIKLSQFIPDYCLVYNRLNTDPAKMVNGKDLDWETEIKTATPHAAVPLESNHPMYIIYTSGTTGEPKALVMGTGNSIVTDKYFLKYVGGVERKDCFWMFSDFGWISGQTLMTYGPLLVGCTMILYEGKPLTKEAMTEVAKIINRHNVAVFGSMPTFFRIMKKLCLEADIKLPYKQLKGICIGGETSNAELRQWISKIFSSPIHNVWGQSELGPLVTGYHYGYYRPNYDESMPNFSINPKIINSKTEEQLPNGTVGRIILKTPISPSAALTIYKNHQKYLDLFFTKYPGYFDTMDYGMLNDDGSLQVLSRSESIIEISSTYICGGVIEEVCLQNEYLLNAAVVPLDDPVTGQVPIAFLEVKNDKPITNNEVIENIIELVTKKVGDFVCFKNAVIVPELPKNRAGKLLRYSLLYMANGKQWTMPVNVEKPHVFKEIYDVLINAGYKASLP 633

HSP 2 Score: 126.716 bits (317), Expect = 2.400e-30
Identity = 57/81 (70.37%), Postives = 71/81 (87.65%), Query Frame = 2 
Query:   11 NATEKVSYKQLLTLVENLAGVMVNKCHVQKGDRVLLYVPVTVQGIAAILACTRIGAVYVVTFGGFAPDLIAARIKHTEPKI 253
            + +E+++Y+QLLTLVE+LAGVMVNKCHV+KGDRVLLY PVT+QG+AA+LACTRIGAVYVV F    PDLIA ++  TEPK+
Sbjct:   83 HTSERMTYQQLLTLVEHLAGVMVNKCHVRKGDRVLLYFPVTIQGVAAMLACTRIGAVYVVIFSSLEPDLIAEKLTSTEPKL 163
[Close]
BLAST of Propionyl-CoA synthetase vs. Planmine SMEST
Match: SMESG000043641.1 (SMESG000043641.1)

HSP 1 Score: 515.768 bits (1327), Expect = 0.000e+0
Identity = 259/475 (54.53%), Postives = 339/475 (71.37%), Query Frame = 3 
Query:  249 KFIITTNFGYEPERTVNYKEIVDEAINKSQFCPDYCIVYNRPNTKCATMIDSRDLDWETEIGKATPYPPVALESGDPIFIIYTSGTLGEPKGLVMTVGGFVVSNKYFMKYVLGLNRRDCWWMFGDYGWISGQTTMTFGPLLIGCSTILYEGKELSSHHFTGIGRIIDQNNVTGFSAAPTYFRFLKNKDSEKDIKPSKAQLRF-VCIGGELSNTKLKEWVSNVFLCPVYDLWGQTELGIIATGPGLGFKNISSNGLSPMLSVHPKVVNSATGEQLPNETIGRMLLKIPLCPSAASSMYNDHDRFLKTYFSKXXXXXXXXXXXXIMNDGTLQILARADDVISVSAGRICGGALEEVCLMNEDLANAAFVALEDPINGQVPFAFLEKKKDRNINNEALIIESVKLVKKEIGSFTLFHQGVVVHDLPKTKTGKILRKTLYNMLNGQDWKTPATVENPKVFEEIYSILIEAGYKASLPND 1670
            K +ITTN  YEP  T+NYKE V++A    QF P Y +VYNRPNT  A M+  +D DWETEI  ATP+  V LES  P+FI YTSGT GEPKG VM  G  +V++KYF+KYV GL R++C+WM  D+GWISGQT MT+GPLL+GC+ IL+EGK ++                                      KP+ A+ +  + +GGELS++ L++W+SN+F CP+Y+LWGQ+ELG +ATGP LGFK  + +   P+L+V  ++VNS TGE+LP ET+GR+LLK P+CPS A ++Y +H RFLK+YF+KY GY+DTMDYGMIMNDG+LQIL+R+DDVI +SA RICGGA EEVCL N+ L NAA V L+DP  GQVP AFLE +KD+ +NN  +I +++  V K++GSFTLF   V+V +LPK KTGKILR +L  M NG+ WK P TV+NP+V++EIY +LI+AGYKA+LP D
Sbjct:  162 KLVITTNIAYEPGTTINYKEQVEKAAKICQFTPAYSLVYNRPNTAPAKMVKGKDFDWETEIKTATPHAAVPLESNHPMFINYTSGTTGEPKGFVMGTGNSIVTDKYFLKYVGGLKRKECFWMLSDFGWISGQTLMTYGPLLVGCTIILFEGKPVN--------------------------------------KPNSAERQLAIYVGGELSDSDLRQWISNIFSCPIYNLWGQSELGPVATGPLLGFKRPNYDLPLPILTVDLRIVNSDTGEELPKETVGRVLLKTPICPSGALTIYKNHQRFLKSYFTKYPGYFDTMDYGMIMNDGSLQILSRSDDVIHISAARICGGAFEEVCLQNDSLLNAAVVPLDDPTAGQVPIAFLEVRKDKAVNNSEVIAKTIDQVTKKMGSFTLFKNAVIVPELPKNKTGKILRYSLSEMANGRVWKIPVTVDNPQVYKEIYDVLIKAGYKATLPID 598

HSP 2 Score: 143.28 bits (360), Expect = 0.000e+0
Identity = 65/83 (78.31%), Postives = 75/83 (90.36%), Query Frame = 2 
Query:    5 VINATEKVSYKQLLTLVENLAGVMVNKCHVQKGDRVLLYVPVTVQGIAAILACTRIGAVYVVTFGGFAPDLIAARIKHTEPKI 253
            + N TEK++Y+QLLTLVE LAGVMVNKCH+ KGDRVLLY PVT+QG+AA+LACTRIGAVYVV F GFAPDLIA R+KHTEPK+
Sbjct:   81 ITNTTEKITYQQLLTLVEQLAGVMVNKCHILKGDRVLLYFPVTIQGMAAMLACTRIGAVYVVIFSGFAPDLIAGRMKHTEPKL 163
[Close]
BLAST of Propionyl-CoA synthetase vs. Planmine SMEST
Match: SMESG000043652.1 (SMESG000043652.1)

HSP 1 Score: 430.254 bits (1105), Expect = 1.724e-143
Identity = 220/474 (46.41%), Postives = 316/474 (66.67%), Query Frame = 3 
Query:  249 KFIITTNFGYEPERTVNYKEIVDEAINKSQFCPDYCIVYNRPNTKCATMIDSRDLDWETEIGKATPYPPVALESGDPIFIIYTSGTLGEPKGLVMTVGGFVVSNKYFMKYVLGLNRRDCWWMFGDYGWISGQTTMTFGPLLIGCSTILYEGKELSSHHFTGIGRIIDQNNVTGFSAAPTYFRFLKNKDSEKDIKPSKAQLRFVCIGGELSNTKLKEWVSNVFLCPVYDLWGQTELGIIATGPGLGFKNISSNGLSPMLSVHPKVVNSATGEQLPNETIGRMLLKIPLCPSAASSMYNDHDRFLKTYFSKXXXXXXXXXXXXIMNDGTLQILARADDVISVSAGRICGGALEEVCLMNEDLANAAFVALEDPINGQVPFAFLEKKKDRNINNEALIIESVKLVKKEIGSFTLFHQGVVVHDLPKTKTGKILRKTLYNMLNGQDWKTPATVENPKVFEEIYSILIEAGYKASLPND 1670
            K I+T+N G++   TV+YK+IVDEAI  SQF  +YC+VYNR N   A+MID RDLDWETE+  A P   + +ES  P+FIIY+SGT GEPKG VMT G  +V++KY +KY+L +  ++ +WMF D+GWI+GQT M++GPLL+GC+TILYEGK  ++ +   + RII+++NV  F A+PT FR LKN +S    K +   LR + I GE S++ L  W+S+ F CPVYD+WGQTEL  + T P L F    ++   PM  + PK++      ++  +  G +LL   L P  A +++ D  R+++TYF+ Y G+YDT D G +  +G L+IL+R+DD+I V++ R CGGA+EE+CL N +L +AA V +++  NGQVPF FL K+KD N+N + ++ ++ +LVK            + V DLPKT T KI R +L  MLN + +K P TVEN  V+++IY  L +AG +ASLP +
Sbjct:  162 KIILTSNVGFDGYNTVDYKQIVDEAIEISQFDVEYCVVYNRGNYPMASMIDIRDLDWETEMMYAVPQRWIPMESDHPLFIIYSSGTTGEPKGFVMTTGAAIVTDKYTIKYLLNIKSKETFWMFSDFGWITGQTVMSYGPLLMGCTTILYEGKPATTPNPQEMARIINEHNVASFMASPTIFRILKNYESIPTFKITINYLRAIYIAGEKSDSNLIRWISDAFNCPVYDIWGQTELNGVVTSPALYFGKPVNSVPVPMFGIKPKLI--YPNPEILGDAFGEILLTAGLSPVFAETLFKDDKRYIETYFTTYQGFYDTKDCGRMTEEGGLEILSRSDDIIKVNSMRFCGGAIEEICLKNHELVSAAVVGVDNEENGQVPFGFLVKRKDSNVNEKDIMNKTKELVKHHYNGLARLENLIFVADLPKTNTHKISRCSLSAMLNRKIFKIPPTVENRNVYQDIYETLRQAGIEASLPKE 633

HSP 2 Score: 97.0561 bits (240), Expect = 7.749e-21
Identity = 42/84 (50.00%), Postives = 60/84 (71.43%), Query Frame = 2 
Query:    2 PVINATEKVSYKQLLTLVENLAGVMVNKCHVQKGDRVLLYVPVTVQGIAAILACTRIGAVYVVTFGGFAPDLIAARIKHTEPKI 253
            PV    +K++Y+ LL  VE L+GV+ ++C+ + GDRVLLY PV  Q + A+LAC+RIG +Y V +GGF  + +A RI+H EPKI
Sbjct:   80 PVTRTVQKITYQSLLESVEKLSGVLADRCNTKAGDRVLLYFPVIPQCLIAMLACSRIGVIYTVIYGGFPAEEVADRIRHIEPKI 163
The following BLAST results are available for this feature:
BLAST of Propionyl-CoA synthetase vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ACSS31.604e-9836.36acyl-CoA synthetase short chain family member 3 [S... [more]
ACSS31.714e-9836.36acyl-CoA synthetase short chain family member 3 [S... [more]
ACSS12.348e-5829.05acyl-CoA synthetase short chain family member 1 [S... [more]
ACSS28.625e-5831.10acyl-CoA synthetase short chain family member 2 [S... [more]
ACSS17.091e-5729.05acyl-CoA synthetase short chain family member 1 [S... [more]
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BLAST of Propionyl-CoA synthetase vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
acs-191.718e-3329.50Acetyl-coenzyme A synthetase [Source:UniProtKB/Tr... [more]
acs-192.088e-3329.74Acetyl-coenzyme A synthetase [Source:UniProtKB/Tr... [more]
acs-192.088e-3329.74Acetyl-coenzyme A synthetase [Source:UniProtKB/Tr... [more]
mec-181.149e-823.10pep chromosome:WBcel235:X:4271169:4274229:-1 gene:... [more]
acs-62.552e-622.50Fatty Acid CoA Synthetase family [Source:UniProtK... [more]
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BLAST of Propionyl-CoA synthetase vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CG64328.323e-8032.72gene:FBgn0039184 transcript:FBtr0346110[more]
CG64328.323e-8032.72gene:FBgn0039184 transcript:FBtr0084606[more]
AcCoAS5.531e-5628.42gene:FBgn0012034 transcript:FBtr0333126[more]
AcCoAS5.531e-5628.42gene:FBgn0012034 transcript:FBtr0078413[more]
AcCoAS5.021e-5028.42gene:FBgn0012034 transcript:FBtr0078414[more]
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BLAST of Propionyl-CoA synthetase vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
acss33.882e-9135.81acyl-CoA synthetase short chain family member 3 [S... [more]
acss35.928e-8333.90acyl-CoA synthetase short chain family member 3 [S... [more]
acss16.603e-6428.94acyl-CoA synthetase short chain family member 1 [S... [more]
acss21.555e-5829.92acyl-CoA synthetase short chain family member 2 [S... [more]
acss23.515e-5031.54acyl-CoA synthetase short chain family member 2 [S... [more]
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BLAST of Propionyl-CoA synthetase vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ACSS32.758e-10137.72acyl-CoA synthetase short chain family member 3 [S... [more]
ACSS36.921e-9837.78acyl-CoA synthetase short chain family member 3 [S... [more]
ACSS31.653e-9537.86acyl-CoA synthetase short chain family member 3 [S... [more]
ACSS34.518e-9137.04acyl-CoA synthetase short chain family member 3 [S... [more]
ACSS37.746e-8935.78acyl-CoA synthetase short chain family member 3 [S... [more]
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BLAST of Propionyl-CoA synthetase vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Acss31.044e-9936.79acyl-CoA synthetase short-chain family member 3 [S... [more]
Acss31.778e-6236.00acyl-CoA synthetase short-chain family member 3 [S... [more]
Acss13.442e-6231.54acyl-CoA synthetase short-chain family member 1 [S... [more]
Acss21.531e-5630.61acyl-CoA synthetase short-chain family member 2 [S... [more]
Acss21.823e-5529.82acyl-CoA synthetase short-chain family member 2 [S... [more]
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BLAST of Propionyl-CoA synthetase vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|A0A0G2K047|ACSS3_RAT2.254e-9936.79Acyl-CoA synthetase short-chain family member 3, m... [more]
sp|Q14DH7|ACSS3_MOUSE6.316e-9936.79Acyl-CoA synthetase short-chain family member 3, m... [more]
sp|Q9H6R3|ACSS3_HUMAN7.513e-9836.36Acyl-CoA synthetase short-chain family member 3, m... [more]
sp|Q5REB8|ACSS3_PONAB1.859e-9736.15Acyl-CoA synthetase short-chain family member 3, m... [more]
sp|A7MB45|ACSS3_BOVIN5.290e-9636.58Acyl-CoA synthetase short-chain family member 3, m... [more]
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BLAST of Propionyl-CoA synthetase vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
J7JE355.975e-10236.72PrpE protein OS=Burkholderia cepacia GG4 OX=100984... [more]
A0A235H7916.876e-10036.33Propionyl-CoA synthetase OS=Azospirillum brasilens... [more]
A0A560AK801.527e-9936.12Propionyl-CoA synthetase OS=Azospirillum brasilens... [more]
A0A2K1FU221.818e-9936.12Propionyl-CoA synthetase OS=Azospirillum brasilens... [more]
A0A091UXS71.881e-9936.75Uncharacterized protein (Fragment) OS=Nipponia nip... [more]
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BLAST of Propionyl-CoA synthetase vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
acss24.178e-5729.20acyl-CoA synthetase short chain family member 2 [S... [more]
acss25.923e-5628.68acyl-CoA synthetase short chain family member 2 [S... [more]
acss2l3.214e-5330.08acetyl-coenzyme A synthetase, cytoplasmic-like [So... [more]
acss2l9.346e-5029.29acetyl-coenzyme A synthetase, cytoplasmic-like [So... [more]
acss12.842e-3726.90acyl-CoA synthetase short chain family member 1 [S... [more]
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BLAST of Propionyl-CoA synthetase vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 3
Match NameE-valueIdentityDescription
ENSPMAT00000006895.18.225e-5529.27pep scaffold:Pmarinus_7.0:GL476378:330410:368976:-... [more]
acss29.560e-4730.14acyl-CoA synthetase short chain family member 2 [S... [more]
ENSPMAT00000005866.11.347e-637.10pep scaffold:Pmarinus_7.0:GL495638:310:2296:-1 gen... [more]
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BLAST of Propionyl-CoA synthetase vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 2
Match NameE-valueIdentityDescription
ACS11.222e-6632.02Acetyl-coA synthetase isoform; along with Acs2p, a... [more]
ACS21.274e-6331.28Acetyl-coA synthetase isoform; along with Acs1p, a... [more]
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BLAST of Propionyl-CoA synthetase vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO492932.779e-9333.83Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO391357.015e-6429.77Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO420843.917e-5327.64Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO442124.836e-1125.27Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO379921.596e-822.57Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Propionyl-CoA synthetase vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
acss31.337e-9236.64acyl-CoA synthetase short chain family member 3 [S... [more]
acss33.485e-8936.02acyl-CoA synthetase short chain family member 3 [S... [more]
acss37.723e-8434.70acyl-CoA synthetase short chain family member 3 [S... [more]
acss31.311e-7735.03acyl-CoA synthetase short chain family member 3 [S... [more]
ENSORLT00000021863.21.940e-5729.67acyl-CoA synthetase short chain family member 1 [S... [more]
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BLAST of Propionyl-CoA synthetase vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000043645.14.354e-4598.12SMESG000043645.1[more]
SMESG000043645.16.877e-4598.12SMESG000043645.1[more]
SMESG000043642.12.400e-3053.81SMESG000043642.1[more]
SMESG000043641.10.000e+054.53SMESG000043641.1[more]
SMESG000043652.11.724e-14346.41SMESG000043652.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30029401 ID=SMED30029401|Name=Propionyl-CoA synthetase|organism=Schmidtea mediterranea sexual|type=transcript|length=1810bp
CCCAGTTATTAATGCCACTGAAAAAGTTTCTTATAAACAATTATTAACAT
TGGTAGAAAATTTGGCAGGGGTGATGGTAAATAAATGCCATGTGCAAAAG
GGCGATCGAGTTTTACTGTACGTTCCTGTCACGGTTCAGGGAATTGCGGC
GATATTGGCGTGTACGCGGATTGGGGCTGTCTACGTAGTTACGTTTGGGG
GATTCGCGCCCGATTTGATAGCTGCTAGAATTAAACATACAGAGCCAAAA
ATTCATAATAACCACCAATTTTGGCTATGAACCGGAACGAACTGTCAATT
ATAAAGAAATTGTTGATGAAGCCATTAATAAAAGTCAATTTTGCCCGGAT
TATTGCATTGTTTACAACAGACCCAATACGAAATGCGCGACCATGATAGA
TTCTAGAGATCTGGATTGGGAAACTGAAATCGGAAAGGCGACCCCTTATC
CGCCTGTAGCTTTGGAATCTGGAGATCCTATATTCATTATTTATACATCA
GGAACACTCGGGGAGCCTAAAGGTTTGGTCATGACAGTTGGAGGTTTTGT
CGTTTCTAATAAATATTTCATGAAGTATGTGCTTGGTTTGAATCGTCGAG
ATTGTTGGTGGATGTTTGGTGACTATGGTTGGATTTCTGGTCAAACCACA
ATGACATTTGGACCATTGTTGATAGGTTGTTCAACTATACTATATGAAGG
GAAAGAATTGTCAAGCCACCACTTTACGGGTATTGGTAGGATCATTGATC
AAAATAACGTTACTGGATTTAGTGCAGCTCCAACATATTTTAGATTTTTG
AAAAATAAAGACTCTGAAAAAGATATAAAGCCTTCAAAAGCTCAATTGCG
ATTTGTATGCATAGGGGGTGAATTATCAAATACTAAATTGAAAGAATGGG
TTTCCAATGTCTTTTTATGTCCAGTATACGATTTGTGGGGACAAACTGAA
TTGGGAATAATAGCAACTGGTCCAGGACTTGGATTTAAAAATATAAGTTC
CAATGGACTTTCGCCTATGTTAAGTGTTCATCCAAAAGTAGTCAACAGCG
CCACTGGAGAGCAGCTACCAAACGAAACAATCGGAAGGATGTTATTAAAA
ATCCCACTTTGTCCATCAGCTGCTTCTAGTATGTACAATGATCATGATAG
GTTTTTAAAGACTTACTTCAGTAAATATGACGGTTACTATGACACTATGG
ATTATGGAATGATAATGAATGATGGCACTCTGCAAATACTAGCCAGGGCA
GATGATGTAATCAGCGTTTCAGCAGGGCGAATCTGTGGAGGTGCTTTAGA
AGAAGTATGTCTAATGAACGAAGACTTAGCAAACGCAGCTTTTGTCGCTT
TGGAAGATCCAATAAATGGTCAAGTTCCTTTTGCATTTCTCGAAAAGAAA
AAAGACCGAAATATAAATAATGAAGCACTAATTATAGAAAGTGTAAAATT
GGTGAAAAAAGAAATCGGTTCATTCACTCTATTTCACCAAGGTGTCGTTG
TGCACGATTTACCAAAGACTAAAACTGGGAAAATTCTTAGAAAAACACTT
TATAATATGTTAAATGGTCAAGATTGGAAAACTCCCGCAACGGTTGAGAA
TCCAAAGGTTTTCGAAGAAATATATTCCATCTTGATTGAAGCTGGTTACA
AAGCCTCTTTACCAAATGATAATTCGTTAAATATGTGAAATTATTTCTTA
TTCGTATTGGAAATTTTAATTTTCACCTATACCTCGCTTTGAGTACTTAA
TTGGTTCCGTAAAAAATTCTACGCAAACTGAATCACCGGTTTATTTTTGT
CAATAGAAAA
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protein sequence of SMED30029401-orf-1

>SMED30029401-orf-1 ID=SMED30029401-orf-1|Name=SMED30029401-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=432bp
MIDSRDLDWETEIGKATPYPPVALESGDPIFIIYTSGTLGEPKGLVMTVG
GFVVSNKYFMKYVLGLNRRDCWWMFGDYGWISGQTTMTFGPLLIGCSTIL
YEGKELSSHHFTGIGRIIDQNNVTGFSAAPTYFRFLKNKDSEKDIKPSKA
QLRFVCIGGELSNTKLKEWVSNVFLCPVYDLWGQTELGIIATGPGLGFKN
ISSNGLSPMLSVHPKVVNSATGEQLPNETIGRMLLKIPLCPSAASSMYND
HDRFLKTYFSKYDGYYDTMDYGMIMNDGTLQILARADDVISVSAGRICGG
ALEEVCLMNEDLANAAFVALEDPINGQVPFAFLEKKKDRNINNEALIIES
VKLVKKEIGSFTLFHQGVVVHDLPKTKTGKILRKTLYNMLNGQDWKTPAT
VENPKVFEEIYSILIEAGYKASLPNDNSLNM*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:0050218propionate-CoA ligase activity
GO:0003824catalytic activity
GO:0003987acetate-CoA ligase activity
GO:0016874ligase activity
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
PLANA:0002089reproductive organ
Vocabulary: INTERPRO
TermDefinition
IPR042099AMP-dep_Synthh-like_sf
IPR000873AMP-dep_Synth/Lig
IPR025110AMP-bd_C
Vocabulary: biological process
TermDefinition
GO:0008152metabolic process
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000873AMP-dependent synthetase/ligasePFAMPF00501AMP-bindingcoord: 5..87
e-value: 5.2E-16
score: 58.3
IPR042099AMP-dependent synthetase-like superfamilyGENE3DG3DSA:3.40.50.12780coord: 2..91
e-value: 2.3E-17
score: 64.7
NoneNo IPR availablePANTHERPTHR43347FAMILY NOT NAMEDcoord: 1..92
NoneNo IPR availableCDDcd05967PrpEcoord: 1..91
e-value: 1.98477E-28
score: 104.32
NoneNo IPR availableSUPERFAMILYSSF56801Acetyl-CoA synthetase-likecoord: 7..91
NoneNo IPR availableTMHMMTMhelixcoord: 37..59