40S ribosomal protein S16

Overview
Name40S ribosomal protein S16
Smed IDSMED30029302
Length (bp)533
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of 40S ribosomal protein S16 (SMED30029302) t-SNE clustered cells

Violin plots show distribution of expression levels for 40S ribosomal protein S16 (SMED30029302) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of 40S ribosomal protein S16 (SMED30029302) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for 40S ribosomal protein S16 (SMED30029302) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30029302

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 15

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30029302SMESG000018349.1 SMESG000018333.1 SmedASXL_003114SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015
FACS sorted cell population asexual adult RNA-sequencing evidence
pharynxSMED30029302SMESG000018349.1 SMESG000018333.1 dd_Smed_v4_170_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
protonephridiaSMED30029302SMESG000018349.1 SMESG000018333.1 dd_Smed_v4_170_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30029302SMESG000018349.1 SMESG000018333.1 dd_Smed_v4_170_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30029302SMESG000018349.1 SMESG000018333.1 dd_Smed_v4_170_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30029302SMESG000018349.1 SMESG000018333.1 dd_Smed_v4_170_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30029302SMESG000018349.1 SMESG000018333.1 dd_Smed_v4_170_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30029302SMESG000018349.1 SMESG000018333.1 dd_Smed_v4_170_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30029302SMESG000018349.1 SMESG000018333.1 dd_Smed_v4_170_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymaSMED30029302SMESG000018349.1 SMESG000018333.1 dd_Smed_v4_170_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30029302SMESG000018349.1 SMESG000018333.1 dd_Smed_v4_170_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30029302SMESG000018349.1 SMESG000018333.1 dd_Smed_v4_170_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30029302SMESG000018349.1 SMESG000018333.1 dd_Smed_v4_170_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
progenitor cellSMED30029302SMESG000018349.1 SMESG000018333.1 dd_Smed_v6_170_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30029302SMESG000018349.1 SMESG000018333.1 dd_Smed_v6_170_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of 40S ribosomal protein S16 vs. Ensembl Human
Match: RPS16 (ribosomal protein S16 [Source:HGNC Symbol;Acc:HGNC:10396])

HSP 1 Score: 202.986 bits (515), Expect = 3.525e-67
Identity = 95/138 (68.84%), Postives = 119/138 (86.23%), Query Frame = 3
Query:   93 SVQVFGRKKTATAVAQCKAGKGNIRVNGYPINLVQPTPLQAKLQEPLLILGEERVKQLDIKIRVSGGGKNSQIYAIRQALAKSVVAYYQKFVDEQSKRELKESFIRYDRSLLVADPRRCEAKKFGGPGARARYQKSYR 506
            SVQVFGRKKTATAVA CK G G I+VNG P+ +++P  LQ KL EP+L+LG+ER   +DI++RV GGG  +QIYAIRQ+++K++VAYYQK+VDE SK+E+K+  I+YDR+LLVADPRRCE+KKFGGPGARARYQKSYR
Sbjct:    9 SVQVFGRKKTATAVAHCKRGNGLIKVNGRPLEMIEPRTLQYKLLEPVLLLGKERFAGVDIRVRVKGGGHVAQIYAIRQSISKALVAYYQKYVDEASKKEIKDILIQYDRTLLVADPRRCESKKFGGPGARARYQKSYR 146          
BLAST of 40S ribosomal protein S16 vs. Ensembl Human
Match: RPS16 (ribosomal protein S16 [Source:HGNC Symbol;Acc:HGNC:10396])

HSP 1 Score: 174.866 bits (442), Expect = 2.484e-56
Identity = 88/138 (63.77%), Postives = 107/138 (77.54%), Query Frame = 3
Query:   93 SVQVFGRKKTATAVAQCKAGKGNIRVNGYPINLVQPTPLQAKLQEPLLILGEERVKQLDIKIRVSGGGKNSQIYAIRQALAKSVVAYYQKFVDEQSKRELKESFIRYDRSLLVADPRRCEAKKFGGPGARARYQKSYR 506
            SVQVFGRKKTATAVA CK G G I                 KL EP+L+LG+ER   +DI++RV GGG  +QIYAIRQ+++K++VAYYQK+VDE SK+E+K+  I+YDR+LLVADPRRCE+KKFGGPGARARYQKSYR
Sbjct:    9 SVQVFGRKKTATAVAHCKRGNGLI-----------------KLLEPVLLLGKERFAGVDIRVRVKGGGHVAQIYAIRQSISKALVAYYQKYVDEASKKEIKDILIQYDRTLLVADPRRCESKKFGGPGARARYQKSYR 129          
BLAST of 40S ribosomal protein S16 vs. Ensembl Human
Match: RPS16 (ribosomal protein S16 [Source:HGNC Symbol;Acc:HGNC:10396])

HSP 1 Score: 120.939 bits (302), Expect = 1.630e-35
Identity = 57/90 (63.33%), Postives = 74/90 (82.22%), Query Frame = 3
Query:   93 SVQVFGRKKTATAVAQCKAGKGNIRVNGYPINLVQPTPLQAKLQEPLLILGEERVKQLDIKIRVSGGGKNSQIYAIRQALAKSVVAYYQK 362
            SVQVFGRKKTATAVA CK G G I+VNG P+ +++P  LQ KL EP+L+LG+ER   +DI++RV GGG  +QIYAIRQ+++K++VAYYQK
Sbjct:    9 SVQVFGRKKTATAVAHCKRGNGLIKVNGRPLEMIEPRTLQYKLLEPVLLLGKERFAGVDIRVRVKGGGHVAQIYAIRQSISKALVAYYQK 98          
BLAST of 40S ribosomal protein S16 vs. Ensembl Human
Match: RPS16 (ribosomal protein S16 [Source:HGNC Symbol;Acc:HGNC:10396])

HSP 1 Score: 104.375 bits (259), Expect = 4.343e-29
Identity = 48/81 (59.26%), Postives = 65/81 (80.25%), Query Frame = 3
Query:  120 TATAVAQCKAGKGNIRVNGYPINLVQPTPLQAKLQEPLLILGEERVKQLDIKIRVSGGGKNSQIYAIRQALAKSVVAYYQK 362
            TATAVA CK G G I+VNG P+ +++P  LQ KL EP+L+LG+ER   +DI++RV GGG  +QIYAIRQ+++K++VAYYQK
Sbjct:    1 TATAVAHCKRGNGLIKVNGRPLEMIEPRTLQYKLLEPVLLLGKERFAGVDIRVRVKGGGHVAQIYAIRQSISKALVAYYQK 81          
BLAST of 40S ribosomal protein S16 vs. Ensembl Human
Match: RPS16 (ribosomal protein S16 [Source:HGNC Symbol;Acc:HGNC:10396])

HSP 1 Score: 100.138 bits (248), Expect = 1.015e-26
Identity = 48/81 (59.26%), Postives = 60/81 (74.07%), Query Frame = 3
Query:   93 SVQVFGRKKTATAVAQCKAGKGNIRVNGYPINLVQPTPLQAKLQEPLLILGEERVKQLDIKIRVSGGGKNSQIYAIRQALA 335
            SVQVFGRKKTATAVA CK G G I+VNG P+ +++P  LQ KL EP+L+LG+ER   +DI++RV GGG  +QIY   Q L 
Sbjct:    9 SVQVFGRKKTATAVAHCKRGNGLIKVNGRPLEMIEPRTLQYKLLEPVLLLGKERFAGVDIRVRVKGGGHVAQIYGESQELG 89          
BLAST of 40S ribosomal protein S16 vs. Ensembl Celegans
Match: rps-16 (40S ribosomal protein S16 [Source:UniProtKB/Swiss-Prot;Acc:Q22054])

HSP 1 Score: 208.764 bits (530), Expect = 6.580e-70
Identity = 100/143 (69.93%), Postives = 122/143 (85.31%), Query Frame = 3
Query:   78 STSVPSVQVFGRKKTATAVAQCKAGKGNIRVNGYPINLVQPTPLQAKLQEPLLILGEERVKQLDIKIRVSGGGKNSQIYAIRQALAKSVVAYYQKFVDEQSKRELKESFIRYDRSLLVADPRRCEAKKFGGPGARARYQKSYR 506
            +++V SVQ FGRKKTATAVA CK G+G I+VNG P+  ++P  L+ KLQEPLL++G+ER + +DI+IRVSGGG  +QIYA+RQALAK++VAYY K+VDEQSKRELK  F  YD+SLLVADPRR E+KKFGGPGARARYQKSYR
Sbjct:    2 TSTVQSVQTFGRKKTATAVAHCKKGQGLIKVNGRPLEFLEPQILRIKLQEPLLLVGKERFQDVDIRIRVSGGGHVAQIYAVRQALAKALVAYYHKYVDEQSKRELKNIFAAYDKSLLVADPRRRESKKFGGPGARARYQKSYR 144          
BLAST of 40S ribosomal protein S16 vs. Ensembl Celegans
Match: rps-16 (40S ribosomal protein S16 [Source:UniProtKB/Swiss-Prot;Acc:Q22054])

HSP 1 Score: 208.764 bits (530), Expect = 6.580e-70
Identity = 100/143 (69.93%), Postives = 122/143 (85.31%), Query Frame = 3
Query:   78 STSVPSVQVFGRKKTATAVAQCKAGKGNIRVNGYPINLVQPTPLQAKLQEPLLILGEERVKQLDIKIRVSGGGKNSQIYAIRQALAKSVVAYYQKFVDEQSKRELKESFIRYDRSLLVADPRRCEAKKFGGPGARARYQKSYR 506
            +++V SVQ FGRKKTATAVA CK G+G I+VNG P+  ++P  L+ KLQEPLL++G+ER + +DI+IRVSGGG  +QIYA+RQALAK++VAYY K+VDEQSKRELK  F  YD+SLLVADPRR E+KKFGGPGARARYQKSYR
Sbjct:    2 TSTVQSVQTFGRKKTATAVAHCKKGQGLIKVNGRPLEFLEPQILRIKLQEPLLLVGKERFQDVDIRIRVSGGGHVAQIYAVRQALAKALVAYYHKYVDEQSKRELKNIFAAYDKSLLVADPRRRESKKFGGPGARARYQKSYR 144          
BLAST of 40S ribosomal protein S16 vs. Ensembl Fly
Match: RpS16 (gene:FBgn0034743 transcript:FBtr0343002)

HSP 1 Score: 198.749 bits (504), Expect = 9.579e-66
Identity = 94/140 (67.14%), Postives = 119/140 (85.00%), Query Frame = 3
Query:   87 VPSVQVFGRKKTATAVAQCKAGKGNIRVNGYPINLVQPTPLQAKLQEPLLILGEERVKQLDIKIRVSGGGKNSQIYAIRQALAKSVVAYYQKFVDEQSKRELKESFIRYDRSLLVADPRRCEAKKFGGPGARARYQKSYR 506
            V +VQVFGRKKTATAVA CK G G ++VNG P+  ++P  LQ KLQEPLL+LG+E+   +DI++RVSGGG  +QIYAIRQA++K++VA+YQK+VDE SK+E+K+  ++YDR+LLV DPRRCE KKFGGPGARARYQKSYR
Sbjct:    9 VQAVQVFGRKKTATAVAYCKRGNGLLKVNGRPLEQIEPKVLQYKLQEPLLLLGKEKFAGVDIRVRVSGGGHVAQIYAIRQAISKALVAFYQKYVDEASKKEIKDILVQYDRTLLVGDPRRCEPKKFGGPGARARYQKSYR 148          
BLAST of 40S ribosomal protein S16 vs. Ensembl Fly
Match: RpS16 (gene:FBgn0034743 transcript:FBtr0071855)

HSP 1 Score: 198.749 bits (504), Expect = 9.579e-66
Identity = 94/140 (67.14%), Postives = 119/140 (85.00%), Query Frame = 3
Query:   87 VPSVQVFGRKKTATAVAQCKAGKGNIRVNGYPINLVQPTPLQAKLQEPLLILGEERVKQLDIKIRVSGGGKNSQIYAIRQALAKSVVAYYQKFVDEQSKRELKESFIRYDRSLLVADPRRCEAKKFGGPGARARYQKSYR 506
            V +VQVFGRKKTATAVA CK G G ++VNG P+  ++P  LQ KLQEPLL+LG+E+   +DI++RVSGGG  +QIYAIRQA++K++VA+YQK+VDE SK+E+K+  ++YDR+LLV DPRRCE KKFGGPGARARYQKSYR
Sbjct:    9 VQAVQVFGRKKTATAVAYCKRGNGLLKVNGRPLEQIEPKVLQYKLQEPLLLLGKEKFAGVDIRVRVSGGGHVAQIYAIRQAISKALVAFYQKYVDEASKKEIKDILVQYDRTLLVGDPRRCEPKKFGGPGARARYQKSYR 148          
BLAST of 40S ribosomal protein S16 vs. Ensembl Fly
Match: mRpS9 (gene:FBgn0037529 transcript:FBtr0081766)

HSP 1 Score: 52.373 bits (124), Expect = 3.392e-8
Identity = 42/127 (33.07%), Postives = 64/127 (50.39%), Query Frame = 3
Query:  114 KKTATAVAQCK-AGKGNIRVNGYPINLVQPTPLQAKLQEPLLILGEERVKQLDIKIRVSGGGKNSQIYAIRQALAKSVVAYYQKFVDEQSKRELKESFIRYDRSLLVADPRRCEAKKFGGPGARARY 491
            +KTA A    +  G G I +NG  I+  +    + +L  PL     E + ++D++  V GGG + Q  AIR  +A S+    + FVD++    ++ +       LL  D RR E KKFG  GAR +Y
Sbjct:  276 RKTARADVTVRLPGTGKISINGKDISYFEDENCREQLLFPLQF--SELLGKVDVEANVEGGGPSGQAGAIRWGIAMSL----RSFVDQEMIESMRLA------GLLTRDYRRRERKKFGQEGARRKY 390          
BLAST of 40S ribosomal protein S16 vs. Ensembl Zebrafish
Match: rps16 (ribosomal protein S16 [Source:ZFIN;Acc:ZDB-GENE-050506-107])

HSP 1 Score: 190.274 bits (482), Expect = 2.216e-62
Identity = 97/144 (67.36%), Postives = 121/144 (84.03%), Query Frame = 3
Query:   75 ASTSVPSVQVFGRKKTATAVAQCKAGKGNIRVNGYPINLVQPTPLQAKLQEPLLILGEERVKQLDIKIRVSGGGKNSQIYAIRQALAKSVVAYYQKFVDEQSKRELKESFIRYDRSLLVADPRRCEAKKFGGPGARARYQKSYR 506
            A   + SVQVFGRKKTATAVA CK G G I+VNG P+ +++P  LQ KL EPLL+LG+ER   +DI++RV GGG  +Q+YAIRQA++K++VAYYQK+VDE SK+E+K+  I+YDR+LLVADPRRCE+KKFGGPGARARYQKSYR
Sbjct:    3 AKGPLQSVQVFGRKKTATAVAHCKRGNGLIKVNGRPLEMIEPVTLQYKLLEPLLLLGKERFAGVDIRVRVKGGGHVAQVYAIRQAISKALVAYYQKYVDEASKKEIKDILIQYDRTLLVADPRRCESKKFGGPGARARYQKSYR 146          
BLAST of 40S ribosomal protein S16 vs. Ensembl Zebrafish
Match: rps16 (ribosomal protein S16 [Source:ZFIN;Acc:ZDB-GENE-050506-107])

HSP 1 Score: 190.274 bits (482), Expect = 2.216e-62
Identity = 97/144 (67.36%), Postives = 121/144 (84.03%), Query Frame = 3
Query:   75 ASTSVPSVQVFGRKKTATAVAQCKAGKGNIRVNGYPINLVQPTPLQAKLQEPLLILGEERVKQLDIKIRVSGGGKNSQIYAIRQALAKSVVAYYQKFVDEQSKRELKESFIRYDRSLLVADPRRCEAKKFGGPGARARYQKSYR 506
            A   + SVQVFGRKKTATAVA CK G G I+VNG P+ +++P  LQ KL EPLL+LG+ER   +DI++RV GGG  +Q+YAIRQA++K++VAYYQK+VDE SK+E+K+  I+YDR+LLVADPRRCE+KKFGGPGARARYQKSYR
Sbjct:    3 AKGPLQSVQVFGRKKTATAVAHCKRGNGLIKVNGRPLEMIEPVTLQYKLLEPLLLLGKERFAGVDIRVRVKGGGHVAQVYAIRQAISKALVAYYQKYVDEASKKEIKDILIQYDRTLLVADPRRCESKKFGGPGARARYQKSYR 146          
BLAST of 40S ribosomal protein S16 vs. Ensembl Zebrafish
Match: mrps9 (mitochondrial ribosomal protein S9 [Source:ZFIN;Acc:ZDB-GENE-060825-257])

HSP 1 Score: 53.5286 bits (127), Expect = 2.207e-8
Identity = 43/132 (32.58%), Postives = 72/132 (54.55%), Query Frame = 3
Query:  108 GRKKTATA-VAQCKAGKGNIRVNGYPINLVQPTPL---QAKLQEPLLILGEERVKQLDIKIRVSGGGKNSQIYAIRQALAKSVVAYYQKFVDEQSKRELKESFIRYDRSLLVADPRRCEAKKFGGPGARARY 491
            GR+K+A A V    +G GNI +NG   N +   P+   + +L  PL  +G   + + DI+  V G G++SQ  A+R A+++++++    FV E    +++++       LL ADPR  E KK G   AR ++
Sbjct:  263 GRRKSAKARVTLIDSGSGNITING--TNYLHYFPVLQDREQLMFPLHFVG--LLGRFDIEGSVEGSGRSSQAGAVRLAISRALLS----FVSEGEMEKMRQA------GLLTADPRIKERKKPGQEKARKKF 380          
BLAST of 40S ribosomal protein S16 vs. Ensembl Xenopus
Match: rps16 (ribosomal protein S16 [Source:NCBI gene;Acc:496563])

HSP 1 Score: 205.682 bits (522), Expect = 3.036e-68
Identity = 97/144 (67.36%), Postives = 121/144 (84.03%), Query Frame = 3
Query:   75 ASTSVPSVQVFGRKKTATAVAQCKAGKGNIRVNGYPINLVQPTPLQAKLQEPLLILGEERVKQLDIKIRVSGGGKNSQIYAIRQALAKSVVAYYQKFVDEQSKRELKESFIRYDRSLLVADPRRCEAKKFGGPGARARYQKSYR 506
            A   + SVQVFGRKKTATAVA CK G G I+VNG P+ +++P  LQ KL EP+L+LG+ER   +DI++RV GGG  +Q+YAIRQA++KS+VAYYQK+VDE SK+E+K+  I+YDR+LLVADPRRCE+KKFGGPGARARYQKSYR
Sbjct:    3 AKGPLQSVQVFGRKKTATAVAHCKRGNGLIKVNGRPLEMIEPATLQYKLLEPVLLLGKERFAGVDIRVRVKGGGHVAQVYAIRQAISKSLVAYYQKYVDEASKKEIKDILIQYDRTLLVADPRRCESKKFGGPGARARYQKSYR 146          
BLAST of 40S ribosomal protein S16 vs. Ensembl Xenopus
Match: rps16 (ribosomal protein S16 [Source:NCBI gene;Acc:496563])

HSP 1 Score: 189.889 bits (481), Expect = 3.650e-62
Identity = 88/131 (67.18%), Postives = 112/131 (85.50%), Query Frame = 3
Query:  114 KKTATAVAQCKAGKGNIRVNGYPINLVQPTPLQAKLQEPLLILGEERVKQLDIKIRVSGGGKNSQIYAIRQALAKSVVAYYQKFVDEQSKRELKESFIRYDRSLLVADPRRCEAKKFGGPGARARYQKSYR 506
            +KTATAVA CK G G I+VNG P+ +++P  LQ KL EP+L+LG+ER   +DI++RV GGG  +Q+YAIRQA++KS+VAYYQK+VDE SK+E+K+  I+YDR+LLVADPRRCE+KKFGGPGARARYQKSYR
Sbjct:   11 QKTATAVAHCKRGNGLIKVNGRPLEMIEPATLQYKLLEPVLLLGKERFAGVDIRVRVKGGGHVAQVYAIRQAISKSLVAYYQKYVDEASKKEIKDILIQYDRTLLVADPRRCESKKFGGPGARARYQKSYR 141          
BLAST of 40S ribosomal protein S16 vs. Ensembl Xenopus
Match: zeb2 (zinc finger E-box binding homeobox 2 [Source:Xenbase;Acc:XB-GENE-479558])

HSP 1 Score: 54.6842 bits (130), Expect = 9.692e-9
Identity = 46/132 (34.85%), Postives = 74/132 (56.06%), Query Frame = 3
Query:  108 GRKKTATA-VAQCKAGKGNIRVNGYPINLVQP-TPLQAK--LQEPLLILGEERVKQLDIKIRVSGGGKNSQIYAIRQALAKSVVAYYQKFVDEQSKRELKESFIRYDRSLLVADPRRCEAKKFGGPGARARY 491
            G++KTA A V   ++G G + VNG  +N ++  T LQ +  L  P   L  +R+ + D++  V+GGGK+SQ  AIR A ++++++    FV E     ++++       LL  DPR  E KK G  GAR ++
Sbjct:  229 GKRKTALATVVLHESGNGTMVVNG--VNYIEYFTVLQDREQLMFPFQFL--DRLGKHDLECTVAGGGKSSQAGAIRLATSRALLS----FVSENEIEAMRQA------GLLTTDPRVKERKKPGQEGARRKF 346          
BLAST of 40S ribosomal protein S16 vs. Ensembl Xenopus
Match: zeb2 (zinc finger E-box binding homeobox 2 [Source:Xenbase;Acc:XB-GENE-479558])

HSP 1 Score: 54.6842 bits (130), Expect = 1.179e-8
Identity = 46/132 (34.85%), Postives = 74/132 (56.06%), Query Frame = 3
Query:  108 GRKKTATA-VAQCKAGKGNIRVNGYPINLVQP-TPLQAK--LQEPLLILGEERVKQLDIKIRVSGGGKNSQIYAIRQALAKSVVAYYQKFVDEQSKRELKESFIRYDRSLLVADPRRCEAKKFGGPGARARY 491
            G++KTA A V   ++G G + VNG  +N ++  T LQ +  L  P   L  +R+ + D++  V+GGGK+SQ  AIR A ++++++    FV E     ++++       LL  DPR  E KK G  GAR ++
Sbjct:  262 GKRKTALATVVLHESGNGTMVVNG--VNYIEYFTVLQDREQLMFPFQFL--DRLGKHDLECTVAGGGKSSQAGAIRLATSRALLS----FVSENEIEAMRQA------GLLTTDPRVKERKKPGQEGARRKF 379          
BLAST of 40S ribosomal protein S16 vs. Ensembl Mouse
Match: Rps16 (ribosomal protein S16 [Source:MGI Symbol;Acc:MGI:98118])

HSP 1 Score: 202.986 bits (515), Expect = 2.420e-67
Identity = 95/138 (68.84%), Postives = 119/138 (86.23%), Query Frame = 3
Query:   93 SVQVFGRKKTATAVAQCKAGKGNIRVNGYPINLVQPTPLQAKLQEPLLILGEERVKQLDIKIRVSGGGKNSQIYAIRQALAKSVVAYYQKFVDEQSKRELKESFIRYDRSLLVADPRRCEAKKFGGPGARARYQKSYR 506
            SVQVFGRKKTATAVA CK G G I+VNG P+ +++P  LQ KL EP+L+LG+ER   +DI++RV GGG  +QIYAIRQ+++K++VAYYQK+VDE SK+E+K+  I+YDR+LLVADPRRCE+KKFGGPGARARYQKSYR
Sbjct:    9 SVQVFGRKKTATAVAHCKRGNGLIKVNGRPLEMIEPRTLQYKLLEPVLLLGKERFAGVDIRVRVKGGGHVAQIYAIRQSISKALVAYYQKYVDEASKKEIKDILIQYDRTLLVADPRRCESKKFGGPGARARYQKSYR 146          
BLAST of 40S ribosomal protein S16 vs. Ensembl Mouse
Match: Mrps9 (mitochondrial ribosomal protein S9 [Source:MGI Symbol;Acc:MGI:1916777])

HSP 1 Score: 59.3066 bits (142), Expect = 2.304e-10
Identity = 46/130 (35.38%), Postives = 71/130 (54.62%), Query Frame = 3
Query:  108 GRKKTATAVAQC-KAGKGNIRVNGYPINLVQP-TPLQAKLQEPLLILGEERVKQLDIKIRVSGGGKNSQIYAIRQALAKSVVAYYQKFVDEQSKRELKESFIRYDRSLLVADPRRCEAKKFGGPGARARY 491
            GR+K+ATA A   + G G I VNG    +  P T  + +L  P   L  +R+++ D+   VSGGG+++Q  AIR A+A+++ +    FV E     ++++       LL  DPR  E KK G  GAR ++
Sbjct:  268 GRRKSATAQAVVYEHGSGKIHVNGVDYLIYFPITQDREQLMFPFHFL--DRLERHDVTCTVSGGGRSAQAGAIRLAMARALCS----FVTEDEVEWMRQA------GLLTPDPRIRERKKPGQEGARRKF 385          
BLAST of 40S ribosomal protein S16 vs. UniProt/SwissProt
Match: sp|Q22054|RS16_CAEEL (40S ribosomal protein S16 OS=Caenorhabditis elegans OX=6239 GN=rps-16 PE=1 SV=3)

HSP 1 Score: 208.764 bits (530), Expect = 8.370e-69
Identity = 100/143 (69.93%), Postives = 122/143 (85.31%), Query Frame = 3
Query:   78 STSVPSVQVFGRKKTATAVAQCKAGKGNIRVNGYPINLVQPTPLQAKLQEPLLILGEERVKQLDIKIRVSGGGKNSQIYAIRQALAKSVVAYYQKFVDEQSKRELKESFIRYDRSLLVADPRRCEAKKFGGPGARARYQKSYR 506
            +++V SVQ FGRKKTATAVA CK G+G I+VNG P+  ++P  L+ KLQEPLL++G+ER + +DI+IRVSGGG  +QIYA+RQALAK++VAYY K+VDEQSKRELK  F  YD+SLLVADPRR E+KKFGGPGARARYQKSYR
Sbjct:    2 TSTVQSVQTFGRKKTATAVAHCKKGQGLIKVNGRPLEFLEPQILRIKLQEPLLLVGKERFQDVDIRIRVSGGGHVAQIYAVRQALAKALVAYYHKYVDEQSKRELKNIFAAYDKSLLVADPRRRESKKFGGPGARARYQKSYR 144          
BLAST of 40S ribosomal protein S16 vs. UniProt/SwissProt
Match: sp|P62250|RS16_RAT (40S ribosomal protein S16 OS=Rattus norvegicus OX=10116 GN=Rps16 PE=1 SV=2)

HSP 1 Score: 202.986 bits (515), Expect = 1.693e-66
Identity = 95/138 (68.84%), Postives = 119/138 (86.23%), Query Frame = 3
Query:   93 SVQVFGRKKTATAVAQCKAGKGNIRVNGYPINLVQPTPLQAKLQEPLLILGEERVKQLDIKIRVSGGGKNSQIYAIRQALAKSVVAYYQKFVDEQSKRELKESFIRYDRSLLVADPRRCEAKKFGGPGARARYQKSYR 506
            SVQVFGRKKTATAVA CK G G I+VNG P+ +++P  LQ KL EP+L+LG+ER   +DI++RV GGG  +QIYAIRQ+++K++VAYYQK+VDE SK+E+K+  I+YDR+LLVADPRRCE+KKFGGPGARARYQKSYR
Sbjct:    9 SVQVFGRKKTATAVAHCKRGNGLIKVNGRPLEMIEPRTLQYKLLEPVLLLGKERFAGVDIRVRVKGGGHVAQIYAIRQSISKALVAYYQKYVDEASKKEIKDILIQYDRTLLVADPRRCESKKFGGPGARARYQKSYR 146          
BLAST of 40S ribosomal protein S16 vs. UniProt/SwissProt
Match: sp|Q29201|RS16_PIG (40S ribosomal protein S16 OS=Sus scrofa OX=9823 GN=RPS16 PE=1 SV=4)

HSP 1 Score: 202.986 bits (515), Expect = 1.693e-66
Identity = 95/138 (68.84%), Postives = 119/138 (86.23%), Query Frame = 3
Query:   93 SVQVFGRKKTATAVAQCKAGKGNIRVNGYPINLVQPTPLQAKLQEPLLILGEERVKQLDIKIRVSGGGKNSQIYAIRQALAKSVVAYYQKFVDEQSKRELKESFIRYDRSLLVADPRRCEAKKFGGPGARARYQKSYR 506
            SVQVFGRKKTATAVA CK G G I+VNG P+ +++P  LQ KL EP+L+LG+ER   +DI++RV GGG  +QIYAIRQ+++K++VAYYQK+VDE SK+E+K+  I+YDR+LLVADPRRCE+KKFGGPGARARYQKSYR
Sbjct:    9 SVQVFGRKKTATAVAHCKRGNGLIKVNGRPLEMIEPRTLQYKLLEPVLLLGKERFAGVDIRVRVKGGGHVAQIYAIRQSISKALVAYYQKYVDEASKKEIKDILIQYDRTLLVADPRRCESKKFGGPGARARYQKSYR 146          
BLAST of 40S ribosomal protein S16 vs. UniProt/SwissProt
Match: sp|P14131|RS16_MOUSE (40S ribosomal protein S16 OS=Mus musculus OX=10090 GN=Rps16 PE=1 SV=4)

HSP 1 Score: 202.986 bits (515), Expect = 1.693e-66
Identity = 95/138 (68.84%), Postives = 119/138 (86.23%), Query Frame = 3
Query:   93 SVQVFGRKKTATAVAQCKAGKGNIRVNGYPINLVQPTPLQAKLQEPLLILGEERVKQLDIKIRVSGGGKNSQIYAIRQALAKSVVAYYQKFVDEQSKRELKESFIRYDRSLLVADPRRCEAKKFGGPGARARYQKSYR 506
            SVQVFGRKKTATAVA CK G G I+VNG P+ +++P  LQ KL EP+L+LG+ER   +DI++RV GGG  +QIYAIRQ+++K++VAYYQK+VDE SK+E+K+  I+YDR+LLVADPRRCE+KKFGGPGARARYQKSYR
Sbjct:    9 SVQVFGRKKTATAVAHCKRGNGLIKVNGRPLEMIEPRTLQYKLLEPVLLLGKERFAGVDIRVRVKGGGHVAQIYAIRQSISKALVAYYQKYVDEASKKEIKDILIQYDRTLLVADPRRCESKKFGGPGARARYQKSYR 146          
BLAST of 40S ribosomal protein S16 vs. UniProt/SwissProt
Match: sp|P62249|RS16_HUMAN (40S ribosomal protein S16 OS=Homo sapiens OX=9606 GN=RPS16 PE=1 SV=2)

HSP 1 Score: 202.986 bits (515), Expect = 1.693e-66
Identity = 95/138 (68.84%), Postives = 119/138 (86.23%), Query Frame = 3
Query:   93 SVQVFGRKKTATAVAQCKAGKGNIRVNGYPINLVQPTPLQAKLQEPLLILGEERVKQLDIKIRVSGGGKNSQIYAIRQALAKSVVAYYQKFVDEQSKRELKESFIRYDRSLLVADPRRCEAKKFGGPGARARYQKSYR 506
            SVQVFGRKKTATAVA CK G G I+VNG P+ +++P  LQ KL EP+L+LG+ER   +DI++RV GGG  +QIYAIRQ+++K++VAYYQK+VDE SK+E+K+  I+YDR+LLVADPRRCE+KKFGGPGARARYQKSYR
Sbjct:    9 SVQVFGRKKTATAVAHCKRGNGLIKVNGRPLEMIEPRTLQYKLLEPVLLLGKERFAGVDIRVRVKGGGHVAQIYAIRQSISKALVAYYQKYVDEASKKEIKDILIQYDRTLLVADPRRCESKKFGGPGARARYQKSYR 146          
BLAST of 40S ribosomal protein S16 vs. TrEMBL
Match: A0A553NYG8 (Uncharacterized protein (Fragment) OS=Tigriopus californicus OX=6832 GN=TCAL_10315 PE=3 SV=1)

HSP 1 Score: 217.238 bits (552), Expect = 2.154e-69
Identity = 107/147 (72.79%), Postives = 125/147 (85.03%), Query Frame = 3
Query:   72 MASTSVP--SVQVFGRKKTATAVAQCKAGKGNIRVNGYPINLVQPTPLQAKLQEPLLILGEERVKQLDIKIRVSGGGKNSQIYAIRQALAKSVVAYYQKFVDEQSKRELKESFIRYDRSLLVADPRRCEAKKFGGPGARARYQKSYR 506
            MA    P  SVQVFGRKKTATAVA CK GKG I+VNG P+ LV+P  LQ KLQEP+L+LG++R   +DI+IRV GGG  SQIYAIRQAL+KS+VAYYQKFVDEQSK+E+K+  I++DR+L+VADPRRCEAKKFGGPGARARYQKSYR
Sbjct:   22 MADAKEPLQSVQVFGRKKTATAVAYCKRGKGLIKVNGRPLELVEPRALQYKLQEPMLLLGKDRFADVDIRIRVKGGGHTSQIYAIRQALSKSLVAYYQKFVDEQSKQEIKDILIQFDRTLMVADPRRCEAKKFGGPGARARYQKSYR 168          
BLAST of 40S ribosomal protein S16 vs. TrEMBL
Match: A0A0A9YDC4 (40S ribosomal protein S16 (Fragment) OS=Lygus hesperus OX=30085 GN=RpS16 PE=3 SV=1)

HSP 1 Score: 214.927 bits (546), Expect = 2.342e-68
Identity = 101/140 (72.14%), Postives = 125/140 (89.29%), Query Frame = 3
Query:   87 VPSVQVFGRKKTATAVAQCKAGKGNIRVNGYPINLVQPTPLQAKLQEPLLILGEERVKQLDIKIRVSGGGKNSQIYAIRQALAKSVVAYYQKFVDEQSKRELKESFIRYDRSLLVADPRRCEAKKFGGPGARARYQKSYR 506
            + SVQVFGRKKTATAVA CK+GKGN+RVNG P+++V+P  LQ KL EP+L+LG+ER   +DI++RV+GGG  +QIYAIRQA++KS+VAYYQK+VDE SK+E+K+  I+YDR+LLVADPRRCEAKKFGGPGARARYQKSYR
Sbjct:   37 IHSVQVFGRKKTATAVAYCKSGKGNLRVNGRPLDMVEPRVLQYKLLEPILLLGKERFSGVDIRVRVNGGGHVAQIYAIRQAISKSLVAYYQKYVDEASKKEIKDIVIQYDRTLLVADPRRCEAKKFGGPGARARYQKSYR 176          
BLAST of 40S ribosomal protein S16 vs. TrEMBL
Match: A0A3E2GSE1 (Uncharacterized protein (Fragment) OS=Scytalidium lignicola OX=5539 GN=B7463_g12307 PE=3 SV=1)

HSP 1 Score: 213.772 bits (543), Expect = 2.878e-68
Identity = 104/145 (71.72%), Postives = 125/145 (86.21%), Query Frame = 3
Query:   72 MASTSVPSVQVFGRKKTATAVAQCKAGKGNIRVNGYPINLVQPTPLQAKLQEPLLILGEERVKQLDIKIRVSGGGKNSQIYAIRQALAKSVVAYYQKFVDEQSKRELKESFIRYDRSLLVADPRRCEAKKFGGPGARARYQKSYR 506
            MAST+  SVQ FG+KKTATAVA CKAGKG I+VNG P+NLVQP  L+ K+ EPLLI+G ++   +DI++RV+GGG  SQIYAIRQA+AKS+VAYYQKFVDE SK +LK++F++YDR+LLVAD RRCE KKFGGPGARARYQKSYR
Sbjct:    1 MASTT-QSVQCFGKKKTATAVAHCKAGKGLIKVNGKPLNLVQPEILRFKIYEPLLIVGLDKFSGVDIRVRVTGGGHTSQIYAIRQAIAKSLVAYYQKFVDEHSKNQLKQAFVQYDRTLLVADNRRCEPKKFGGPGARARYQKSYR 144          
BLAST of 40S ribosomal protein S16 vs. TrEMBL
Match: A0A0K8T4K8 (Uncharacterized protein (Fragment) OS=Lygus hesperus OX=30085 PE=3 SV=1)

HSP 1 Score: 214.927 bits (546), Expect = 2.980e-68
Identity = 101/140 (72.14%), Postives = 125/140 (89.29%), Query Frame = 3
Query:   87 VPSVQVFGRKKTATAVAQCKAGKGNIRVNGYPINLVQPTPLQAKLQEPLLILGEERVKQLDIKIRVSGGGKNSQIYAIRQALAKSVVAYYQKFVDEQSKRELKESFIRYDRSLLVADPRRCEAKKFGGPGARARYQKSYR 506
            + SVQVFGRKKTATAVA CK+GKGN+RVNG P+++V+P  LQ KL EP+L+LG+ER   +DI++RV+GGG  +QIYAIRQA++KS+VAYYQK+VDE SK+E+K+  I+YDR+LLVADPRRCEAKKFGGPGARARYQKSYR
Sbjct:   36 IHSVQVFGRKKTATAVAYCKSGKGNLRVNGRPLDMVEPRVLQYKLLEPILLLGKERFSGVDIRVRVNGGGHVAQIYAIRQAISKSLVAYYQKYVDEASKKEIKDIVIQYDRTLLVADPRRCEAKKFGGPGARARYQKSYR 175          
BLAST of 40S ribosomal protein S16 vs. TrEMBL
Match: A0A146M9D1 (40S ribosomal protein S16 (Fragment) OS=Lygus hesperus OX=30085 GN=Rps16 PE=3 SV=1)

HSP 1 Score: 214.927 bits (546), Expect = 3.045e-68
Identity = 101/140 (72.14%), Postives = 125/140 (89.29%), Query Frame = 3
Query:   87 VPSVQVFGRKKTATAVAQCKAGKGNIRVNGYPINLVQPTPLQAKLQEPLLILGEERVKQLDIKIRVSGGGKNSQIYAIRQALAKSVVAYYQKFVDEQSKRELKESFIRYDRSLLVADPRRCEAKKFGGPGARARYQKSYR 506
            + SVQVFGRKKTATAVA CK+GKGN+RVNG P+++V+P  LQ KL EP+L+LG+ER   +DI++RV+GGG  +QIYAIRQA++KS+VAYYQK+VDE SK+E+K+  I+YDR+LLVADPRRCEAKKFGGPGARARYQKSYR
Sbjct:   42 IHSVQVFGRKKTATAVAYCKSGKGNLRVNGRPLDMVEPRVLQYKLLEPILLLGKERFSGVDIRVRVNGGGHVAQIYAIRQAISKSLVAYYQKYVDEASKKEIKDIVIQYDRTLLVADPRRCEAKKFGGPGARARYQKSYR 181          
BLAST of 40S ribosomal protein S16 vs. Ensembl Cavefish
Match: rps16 (ribosomal protein S16 [Source:NCBI gene;Acc:103045159])

HSP 1 Score: 207.994 bits (528), Expect = 1.697e-69
Identity = 98/144 (68.06%), Postives = 121/144 (84.03%), Query Frame = 3
Query:   75 ASTSVPSVQVFGRKKTATAVAQCKAGKGNIRVNGYPINLVQPTPLQAKLQEPLLILGEERVKQLDIKIRVSGGGKNSQIYAIRQALAKSVVAYYQKFVDEQSKRELKESFIRYDRSLLVADPRRCEAKKFGGPGARARYQKSYR 506
            A   + SVQVFGRKKTATAVA CK G G I+VNG P+ ++QP  LQ KL EP+L+LG+ER   +DI+IRV GGG  +Q+YAIRQA++K++VAYYQKFVDE SK+E+K+  ++YDR+LLVADPRRCE+KKFGGPGARARYQKSYR
Sbjct:    3 AKGPIQSVQVFGRKKTATAVAHCKRGNGLIKVNGRPLEMIQPATLQYKLLEPILLLGKERFAGVDIRIRVKGGGHVAQVYAIRQAISKALVAYYQKFVDEASKKEIKDILVQYDRTLLVADPRRCESKKFGGPGARARYQKSYR 146          
BLAST of 40S ribosomal protein S16 vs. Ensembl Cavefish
Match: mrps9 (mitochondrial ribosomal protein S9 [Source:NCBI gene;Acc:103022208])

HSP 1 Score: 53.5286 bits (127), Expect = 1.715e-8
Identity = 44/141 (31.21%), Postives = 77/141 (54.61%), Query Frame = 3
Query:   87 VPSVQVFGRKKTATA---VAQCKAGKGNIRVNGYPINLVQPTPL---QAKLQEPLLILGEERVKQLDIKIRVSGGGKNSQIYAIRQALAKSVVAYYQKFVDEQSKRELKESFIRYDRSLLVADPRRCEAKKFGGPGARARY 491
            V   Q  GR+KTA+A   +  C++G  NI VNG   + +Q  P+   + +L  PL  +G   + + D++  V GGG+++Q  A+R A+++++++    FV       ++++       LL +DPR  E KK G  GAR ++
Sbjct:  255 VAFCQANGRRKTASASITLRDCRSG--NIMVNGK--SYLQYFPILQDREQLMFPLHFVG--MLGRFDVEAHVEGGGRSAQAGALRLAISRALLS----FVSSGEMETMRQA------GLLTSDPRVKERKKPGQAGARKKF 379          
BLAST of 40S ribosomal protein S16 vs. Ensembl Yeast
Match: RPS16B (Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S16 and bacterial S9; RPS16B has a paralog, RPS16A, that arose from the whole genome duplication [Source:SGD;Acc:S000002241])

HSP 1 Score: 193.356 bits (490), Expect = 1.373e-64
Identity = 91/142 (64.08%), Postives = 118/142 (83.10%), Query Frame = 3
Query:   81 TSVPSVQVFGRKKTATAVAQCKAGKGNIRVNGYPINLVQPTPLQAKLQEPLLILGEERVKQLDIKIRVSGGGKNSQIYAIRQALAKSVVAYYQKFVDEQSKRELKESFIRYDRSLLVADPRRCEAKKFGGPGARARYQKSYR 506
            ++VPSVQ FG+KK+ATAVA  KAGKG I+VNG PI LV+P  L+ K+ EPLL++G ++   +DI++RV+GGG  SQ+YAIRQA+AK +VAY+QK+VDEQSK ELK++F  YDR+LL+AD RR E KKFGG GAR+R+QKSYR
Sbjct:    2 SAVPSVQTFGKKKSATAVAHVKAGKGLIKVNGSPITLVEPEILRFKVYEPLLLVGLDKFSNIDIRVRVTGGGHVSQVYAIRQAIAKGLVAYHQKYVDEQSKNELKKAFTSYDRTLLIADSRRPEPKKFGGKGARSRFQKSYR 143          
BLAST of 40S ribosomal protein S16 vs. Ensembl Yeast
Match: RPS16A (Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S16 and bacterial S9; RPS16A has a paralog, RPS16B, that arose from the whole genome duplication [Source:SGD;Acc:S000004751])

HSP 1 Score: 193.356 bits (490), Expect = 1.373e-64
Identity = 91/142 (64.08%), Postives = 118/142 (83.10%), Query Frame = 3
Query:   81 TSVPSVQVFGRKKTATAVAQCKAGKGNIRVNGYPINLVQPTPLQAKLQEPLLILGEERVKQLDIKIRVSGGGKNSQIYAIRQALAKSVVAYYQKFVDEQSKRELKESFIRYDRSLLVADPRRCEAKKFGGPGARARYQKSYR 506
            ++VPSVQ FG+KK+ATAVA  KAGKG I+VNG PI LV+P  L+ K+ EPLL++G ++   +DI++RV+GGG  SQ+YAIRQA+AK +VAY+QK+VDEQSK ELK++F  YDR+LL+AD RR E KKFGG GAR+R+QKSYR
Sbjct:    2 SAVPSVQTFGKKKSATAVAHVKAGKGLIKVNGSPITLVEPEILRFKVYEPLLLVGLDKFSNIDIRVRVTGGGHVSQVYAIRQAIAKGLVAYHQKYVDEQSKNELKKAFTSYDRTLLIADSRRPEPKKFGGKGARSRFQKSYR 143          
BLAST of 40S ribosomal protein S16 vs. Ensembl Nematostella
Match: EDO37119 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SGN4])

HSP 1 Score: 204.527 bits (519), Expect = 1.451e-68
Identity = 95/138 (68.84%), Postives = 122/138 (88.41%), Query Frame = 3
Query:   93 SVQVFGRKKTATAVAQCKAGKGNIRVNGYPINLVQPTPLQAKLQEPLLILGEERVKQLDIKIRVSGGGKNSQIYAIRQALAKSVVAYYQKFVDEQSKRELKESFIRYDRSLLVADPRRCEAKKFGGPGARARYQKSYR 506
            SVQVFGRKKTATAVA CK G G I+VNG P++LV+P+ L+ K++EP+L+LG+ER + +DI++RV GGG  S+IYAIRQA++KS+VAYYQK+VDE SK+E+++  ++YDRSLLVADPRR EAKKFGGPGAR+RYQKSYR
Sbjct:    4 SVQVFGRKKTATAVAFCKRGNGMIKVNGVPLHLVEPSTLRFKVEEPILLLGKERFEGVDIRVRVKGGGHTSRIYAIRQAISKSLVAYYQKYVDEVSKKEIRDILVQYDRSLLVADPRRTEAKKFGGPGARSRYQKSYR 141          
BLAST of 40S ribosomal protein S16 vs. Ensembl Nematostella
Match: EDO26045 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7TD13])

HSP 1 Score: 43.5134 bits (101), Expect = 5.283e-6
Identity = 37/134 (27.61%), Postives = 59/134 (44.03%), Query Frame = 3
Query:  108 GRKKTATAVAQCKAGKGNIRVNGYPINLVQPTPLQAKL-QEPLLILGEERVKQLDIKIRVSGGGKNSQIYAIRQALAKSVVAYYQKFVDEQSKRELKESFIRYDRSLLVADPRRCEAKKFGGPGARARYQKSYR 506
            GR+K++ A      G G I VN          P    + ++PL +   E +   DIK+ V GGG++ Q  AI+  + ++++ +           +LK +  +     +  D R  E KK G   AR R Q S R
Sbjct:   10 GRRKSSVARVXLAKGSGKIIVNDRDFEEYFSRPTNCMIVRQPLTLT--EHLDSFDIKVNVHGGGESGQAGAIKHGITRALIDFNA---------DLKPTLSKA--GFVTRDAREVERKKCGLRKARRRKQFSKR 130          
BLAST of 40S ribosomal protein S16 vs. Ensembl Medaka
Match: rps16 (ribosomal protein S16 [Source:NCBI gene;Acc:101166675])

HSP 1 Score: 205.682 bits (522), Expect = 1.855e-68
Identity = 97/144 (67.36%), Postives = 122/144 (84.72%), Query Frame = 3
Query:   75 ASTSVPSVQVFGRKKTATAVAQCKAGKGNIRVNGYPINLVQPTPLQAKLQEPLLILGEERVKQLDIKIRVSGGGKNSQIYAIRQALAKSVVAYYQKFVDEQSKRELKESFIRYDRSLLVADPRRCEAKKFGGPGARARYQKSYR 506
            A   + SVQVFGRKKTATAVA CK G G I+VNG P+ +V+P+ LQ KL EP+L+LG+ER   +DI++RV GGG  +Q+YAIRQA++K++VAYYQK+VDE SK+E+K+  I+YDR+LLVADPRRCE+KKFGGPGARARYQKSYR
Sbjct:    3 AKGPLQSVQVFGRKKTATAVAHCKRGNGLIKVNGRPLEMVEPSTLQYKLLEPVLLLGKERFAGVDIRVRVKGGGHVAQVYAIRQAISKALVAYYQKYVDEASKKEIKDILIQYDRTLLVADPRRCESKKFGGPGARARYQKSYR 146          
BLAST of 40S ribosomal protein S16 vs. Ensembl Medaka
Match: rps16 (ribosomal protein S16 [Source:NCBI gene;Acc:101166675])

HSP 1 Score: 178.333 bits (451), Expect = 1.839e-57
Identity = 86/144 (59.72%), Postives = 110/144 (76.39%), Query Frame = 3
Query:   75 ASTSVPSVQVFGRKKTATAVAQCKAGKGNIRVNGYPINLVQPTPLQAKLQEPLLILGEERVKQLDIKIRVSGGGKNSQIYAIRQALAKSVVAYYQKFVDEQSKRELKESFIRYDRSLLVADPRRCEAKKFGGPGARARYQKSYR 506
            A   + SVQVFGRKKTATAVA CK G G I+VNG P+ +V+P+ LQ KL EP+L+LG+ER   +DI++RV GGG  +Q+Y       + +   + K+VDE SK+E+K+  I+YDR+LLVADPRRCE+KKFGGPGARARYQKSYR
Sbjct:   21 AKGPLQSVQVFGRKKTATAVAHCKRGNGLIKVNGRPLEMVEPSTLQYKLLEPVLLLGKERFAGVDIRVRVKGGGHVAQVYGKITTPHEVLACCFPKYVDEASKKEIKDILIQYDRTLLVADPRRCESKKFGGPGARARYQKSYR 164          
BLAST of 40S ribosomal protein S16 vs. Ensembl Medaka
Match: mrps9 (mitochondrial ribosomal protein S9 [Source:NCBI gene;Acc:101154927])

HSP 1 Score: 47.7506 bits (112), Expect = 1.894e-6
Identity = 40/129 (31.01%), Postives = 63/129 (48.84%), Query Frame = 3
Query:  111 RKKTATAVAQCKAGKGNIRVNGYPINLVQPTPLQAK--LQEPLLILGEERVKQLDIKIRVSGGGKNSQIYAIRQALAKSVVAYYQKFVDEQSKRELKESFIRYDRSLLVADPRRCEAKKFGGPGARARY 491
            RK + + V     G G + VNG    L  P  LQ +  L  PL   G   + + D++  VSGGG++SQ  A+R A++++++++      E  ++            LL  DPR  E KK G  GAR ++
Sbjct:  309 RKTSHSTVVLRDCGSGRLSVNGQDSLLYFPV-LQDREQLMFPLQFTGT--LGRFDLECTVSGGGRSSQAGALRLAVSRALLSFLSPSEGEMMRQA----------GLLTPDPRVRERKKPGQEGARRKF 424          
BLAST of 40S ribosomal protein S16 vs. Planmine SMEST
Match: SMESG000018349.1 (SMESG000018349.1)

HSP 1 Score: 292.352 bits (747), Expect = 6.319e-103
Identity = 145/145 (100.00%), Postives = 145/145 (100.00%), Query Frame = 3
Query:   72 MASTSVPSVQVFGRKKTATAVAQCKAGKGNIRVNGYPINLVQPTPLQAKLQEPLLILGEERVKQLDIKIRVSGGGKNSQIYAIRQALAKSVVAYYQKFVDEQSKRELKESFIRYDRSLLVADPRRCEAKKFGGPGARARYQKSYR 506
            MASTSVPSVQVFGRKKTATAVAQCKAGKGNIRVNGYPINLVQPTPLQAKLQEPLLILGEERVKQLDIKIRVSGGGKNSQIYAIRQALAKSVVAYYQKFVDEQSKRELKESFIRYDRSLLVADPRRCEAKKFGGPGARARYQKSYR
Sbjct:    1 MASTSVPSVQVFGRKKTATAVAQCKAGKGNIRVNGYPINLVQPTPLQAKLQEPLLILGEERVKQLDIKIRVSGGGKNSQIYAIRQALAKSVVAYYQKFVDEQSKRELKESFIRYDRSLLVADPRRCEAKKFGGPGARARYQKSYR 145          
BLAST of 40S ribosomal protein S16 vs. Planmine SMEST
Match: SMESG000018333.1 (SMESG000018333.1)

HSP 1 Score: 292.352 bits (747), Expect = 6.319e-103
Identity = 145/145 (100.00%), Postives = 145/145 (100.00%), Query Frame = 3
Query:   72 MASTSVPSVQVFGRKKTATAVAQCKAGKGNIRVNGYPINLVQPTPLQAKLQEPLLILGEERVKQLDIKIRVSGGGKNSQIYAIRQALAKSVVAYYQKFVDEQSKRELKESFIRYDRSLLVADPRRCEAKKFGGPGARARYQKSYR 506
            MASTSVPSVQVFGRKKTATAVAQCKAGKGNIRVNGYPINLVQPTPLQAKLQEPLLILGEERVKQLDIKIRVSGGGKNSQIYAIRQALAKSVVAYYQKFVDEQSKRELKESFIRYDRSLLVADPRRCEAKKFGGPGARARYQKSYR
Sbjct:    1 MASTSVPSVQVFGRKKTATAVAQCKAGKGNIRVNGYPINLVQPTPLQAKLQEPLLILGEERVKQLDIKIRVSGGGKNSQIYAIRQALAKSVVAYYQKFVDEQSKRELKESFIRYDRSLLVADPRRCEAKKFGGPGARARYQKSYR 145          
The following BLAST results are available for this feature:
BLAST of 40S ribosomal protein S16 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
RPS163.525e-6768.84ribosomal protein S16 [Source:HGNC Symbol;Acc:HGNC... [more]
RPS162.484e-5663.77ribosomal protein S16 [Source:HGNC Symbol;Acc:HGNC... [more]
RPS161.630e-3563.33ribosomal protein S16 [Source:HGNC Symbol;Acc:HGNC... [more]
RPS164.343e-2959.26ribosomal protein S16 [Source:HGNC Symbol;Acc:HGNC... [more]
RPS161.015e-2659.26ribosomal protein S16 [Source:HGNC Symbol;Acc:HGNC... [more]
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BLAST of 40S ribosomal protein S16 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 2
Match NameE-valueIdentityDescription
rps-166.580e-7069.9340S ribosomal protein S16 [Source:UniProtKB/Swiss... [more]
rps-166.580e-7069.9340S ribosomal protein S16 [Source:UniProtKB/Swiss... [more]
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BLAST of 40S ribosomal protein S16 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 3
Match NameE-valueIdentityDescription
RpS169.579e-6667.14gene:FBgn0034743 transcript:FBtr0343002[more]
RpS169.579e-6667.14gene:FBgn0034743 transcript:FBtr0071855[more]
mRpS93.392e-833.07gene:FBgn0037529 transcript:FBtr0081766[more]
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BLAST of 40S ribosomal protein S16 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 3
Match NameE-valueIdentityDescription
rps162.216e-6267.36ribosomal protein S16 [Source:ZFIN;Acc:ZDB-GENE-05... [more]
rps162.216e-6267.36ribosomal protein S16 [Source:ZFIN;Acc:ZDB-GENE-05... [more]
mrps92.207e-832.58mitochondrial ribosomal protein S9 [Source:ZFIN;Ac... [more]
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BLAST of 40S ribosomal protein S16 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 4
Match NameE-valueIdentityDescription
rps163.036e-6867.36ribosomal protein S16 [Source:NCBI gene;Acc:496563... [more]
rps163.650e-6267.18ribosomal protein S16 [Source:NCBI gene;Acc:496563... [more]
zeb29.692e-934.85zinc finger E-box binding homeobox 2 [Source:Xenba... [more]
zeb21.179e-834.85zinc finger E-box binding homeobox 2 [Source:Xenba... [more]
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BLAST of 40S ribosomal protein S16 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Rps162.420e-6768.84ribosomal protein S16 [Source:MGI Symbol;Acc:MGI:9... [more]
Mrps92.304e-1035.38mitochondrial ribosomal protein S9 [Source:MGI Sym... [more]
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BLAST of 40S ribosomal protein S16 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q22054|RS16_CAEEL8.370e-6969.9340S ribosomal protein S16 OS=Caenorhabditis elegan... [more]
sp|P62250|RS16_RAT1.693e-6668.8440S ribosomal protein S16 OS=Rattus norvegicus OX=... [more]
sp|Q29201|RS16_PIG1.693e-6668.8440S ribosomal protein S16 OS=Sus scrofa OX=9823 GN... [more]
sp|P14131|RS16_MOUSE1.693e-6668.8440S ribosomal protein S16 OS=Mus musculus OX=10090... [more]
sp|P62249|RS16_HUMAN1.693e-6668.8440S ribosomal protein S16 OS=Homo sapiens OX=9606 ... [more]
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BLAST of 40S ribosomal protein S16 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A553NYG82.154e-6972.79Uncharacterized protein (Fragment) OS=Tigriopus ca... [more]
A0A0A9YDC42.342e-6872.1440S ribosomal protein S16 (Fragment) OS=Lygus hesp... [more]
A0A3E2GSE12.878e-6871.72Uncharacterized protein (Fragment) OS=Scytalidium ... [more]
A0A0K8T4K82.980e-6872.14Uncharacterized protein (Fragment) OS=Lygus hesper... [more]
A0A146M9D13.045e-6872.1440S ribosomal protein S16 (Fragment) OS=Lygus hesp... [more]
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BLAST of 40S ribosomal protein S16 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
rps161.697e-6968.06ribosomal protein S16 [Source:NCBI gene;Acc:103045... [more]
mrps91.715e-831.21mitochondrial ribosomal protein S9 [Source:NCBI ge... [more]
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BLAST of 40S ribosomal protein S16 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of 40S ribosomal protein S16 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 2
Match NameE-valueIdentityDescription
RPS16B1.373e-6464.08Protein component of the small (40S) ribosomal sub... [more]
RPS16A1.373e-6464.08Protein component of the small (40S) ribosomal sub... [more]
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BLAST of 40S ribosomal protein S16 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 2
Match NameE-valueIdentityDescription
EDO371191.451e-6868.84Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO260455.283e-627.61Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of 40S ribosomal protein S16 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 3
Match NameE-valueIdentityDescription
rps161.855e-6867.36ribosomal protein S16 [Source:NCBI gene;Acc:101166... [more]
rps161.839e-5759.72ribosomal protein S16 [Source:NCBI gene;Acc:101166... [more]
mrps91.894e-631.01mitochondrial ribosomal protein S9 [Source:NCBI ge... [more]
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BLAST of 40S ribosomal protein S16 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000018349.16.319e-103100.00SMESG000018349.1[more]
SMESG000018333.16.319e-103100.00SMESG000018333.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30029302 ID=SMED30029302|Name=40S ribosomal protein S16|organism=Schmidtea mediterranea sexual|type=transcript|length=533bp
GTTATTGTTTAAATACATTGATTGAATATTTTGTAAAATTTTTAATTTAA
AAAATTATAATATAATACATTATGGCTTCTACTAGTGTTCCAAGTGTTCA
AGTTTTTGGCAGAAAAAAAACTGCAACTGCTGTTGCTCAATGCAAAGCTG
GTAAAGGAAACATTCGTGTCAATGGTTACCCAATTAACCTTGTTCAACCA
ACTCCTTTACAAGCTAAACTCCAAGAACCATTGTTGATTCTTGGCGAGGA
AAGAGTTAAACAATTAGATATTAAAATCAGAGTTAGTGGTGGTGGAAAGA
ACTCACAAATTTATGCCATTAGACAAGCATTGGCTAAATCAGTTGTTGCT
TACTATCAAAAATTTGTTGATGAACAATCCAAAAGAGAATTAAAAGAAAG
TTTCATTCGATACGATAGAAGTCTTCTAGTCGCTGATCCCAGAAGATGTG
AAGCTAAAAAATTCGGTGGTCCAGGTGCAAGAGCCAGATATCAAAAATCA
TACCGTTAAAATATAATAAATATTTATTGGATT
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protein sequence of SMED30029302-orf-1

>SMED30029302-orf-1 ID=SMED30029302-orf-1|Name=SMED30029302-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=146bp
MASTSVPSVQVFGRKKTATAVAQCKAGKGNIRVNGYPINLVQPTPLQAKL
QEPLLILGEERVKQLDIKIRVSGGGKNSQIYAIRQALAKSVVAYYQKFVD
EQSKRELKESFIRYDRSLLVADPRRCEAKKFGGPGARARYQKSYR*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000120progenitor cell
PLANA:0000429neoblast
PLANA:0002032epidermal cell
PLANA:0002109X1 cell
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR014721Ribosomal_S5_D2-typ_fold_subgr
IPR000754Ribosomal_S9
IPR020574Ribosomal_S9_CS
IPR020568Ribosomal_S5_D2-typ_fold
Vocabulary: cellular component
TermDefinition
GO:0005840ribosome
GO:0022627cytosolic small ribosomal subunit
GO:0030529intracellular ribonucleoprotein complex
Vocabulary: biological process
TermDefinition
GO:0006412translation
GO:0000462maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
Vocabulary: molecular function
TermDefinition
GO:0003735structural constituent of ribosome
GO:0005515protein binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000754Ribosomal protein S9PFAMPF00380Ribosomal_S9coord: 13..145
e-value: 2.6E-32
score: 111.9
IPR000754Ribosomal protein S9PANTHERPTHR21569RIBOSOMAL PROTEIN S9coord: 6..145
IPR014721Ribosomal protein S5 domain 2-type fold, subgroupGENE3DG3DSA:3.30.230.10coord: 1..145
e-value: 7.7E-64
score: 216.1
NoneNo IPR availablePANTHERPTHR21569:SF1540S RIBOSOMAL PROTEIN S16coord: 6..145
IPR020574Ribosomal protein S9, conserved sitePROSITEPS00360RIBOSOMAL_S9coord: 73..91
IPR020568Ribosomal protein S5 domain 2-type foldSUPERFAMILYSSF54211Ribosomal protein S5 domain 2-likecoord: 8..145