Rho_N domain-containing protein
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30029260 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 2
Homology
BLAST of Rho_N domain-containing protein vs. Ensembl Celegans
Match: ZC53.4 (pep chromosome:WBcel235:X:1925750:1937689:1 gene:WBGene00022511.1 transcript:ZC53.4.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:ZC53.4) HSP 1 Score: 98.5969 bits (244), Expect = 3.405e-21 Identity = 86/355 (24.23%), Postives = 163/355 (45.92%), Query Frame = 2 Query: 317 IVRAKGIYPYDFVTSLKVLESNYELP---PIWSSVSDDEYATAQEI*TLQNCRTM*DYTLVYLK*DLRIITKEIAYNGLEPLNFYGIPGMSMASALMTLRQPIEVLQDM*MYDFYEGGIRGGI-------TFVNKYYAASSEDTEL----LYIDINNLNGWALSQNLPCDNFVWVHENLNNVLSECMSGNIELLPY---------SYTIEIDIDIPDRLHNFLNDFPIAPQRICPPNSKVEKLMLTHWPKKNHVLYWRLLNLYIKLGAIVVIIHRAIKFKQTPILKT*IKIQI-----CAQTLHVSCIEICTNYLIIVYMVNL*GIGKKRMILRPCNSAEKMIVYDSKPTFRKSI 1297 ++R KG+YPY+++ S K + +LP + +++ + +N R + LR+ L+P ++ PG++ + L + ++ L D+ MY E GIRGGI + VN Y + + +L LY+D NNL GWA+ Q LP +F + ++L ++L+ +L Y E+D++ P +H+ ND P+ P+ + N ++KL T + ++ +V+++ L ++LG + IHR I FK++ L + I++ ++ N + M N+ ++R+ +R +K+ SKP F++ I Sbjct: 1074 LMRKKGVYPYEYMDSFKRFDKT-KLPNRDKFYDKLNNKKCKRKGIADVFENFRAL----------SLRVYQ-------LDPCWYFTSPGLAWDAMLKKTKVELDKLHDVEMYLMIEKGIRGGIVNAVKRYSKVNNKYTKNFNEKQLTNFLLYLDANNLYGWAMCQMLPTGDFKFETQSLISILNGEAEVKSDLKDYINYLKKLDRGCIFEVDLEYPKEIHSRHNDSPLCPENVKIDNHVIKKLCNTLFDREKYVIHYENLLQCLQLGLKLKKIHRIITFKESNWLASYIEMNTNPRKNAKNDFEKDFYKLMNNSVFGKTMENV----RERVDVRLVTDEDKIKKLASKPNFKRRI 1406
BLAST of Rho_N domain-containing protein vs. TrEMBL
Match: A0A2L2YKB9 (Uncharacterized protein (Fragment) OS=Parasteatoda tepidariorum OX=114398 PE=2 SV=1) HSP 1 Score: 191.815 bits (486), Expect = 1.376e-49 Identity = 142/460 (30.87%), Postives = 227/460 (49.35%), Query Frame = 2 Query: 311 GIIVRAKGIYPYDFVTSLKVLESNYELPPIW--------SSVSDDEYATAQEI*TLQNCRTM*DYTLVYLK*DLRIITKEIAYN---------GLEPLNFYGIPGMSMASALMTLRQPIEVLQDM*MYDFYEGGIRGGITFVNKYYA-----------ASSEDTELLYIDINNLNGWALSQNLPCDNFVWVHENLNNVLSECMSGNIELLPYSYTIEIDIDIPDRLHNFLNDFPIAPQRICPPNSKVEKLMLTHWPKKNHVLYWRLLNLYIKLGAIVVIIHRAIKFKQTPILKT*IKIQICAQTLHVSCIE-----ICTNYLIIVYMVNL*GIGKKRMILRPCNSAEKMIVYDSKPTFRKSIKIDDYLIAAHLNRGSICLDRPNYIDQSVLDLSKLRIYHLQ*NELA-KYREELNCDINIVALDTDSFFLEVLNCKLDTILPTIIRNKLLDTSNYDPVHP 1588 +I+R KG++PYD++ S LPP ++DDEYA A EI N T+ DY+ YLK D+ ++ +I N L+P +Y PG+S + L + IE+ QD M F E G RGGI+ +K YA +S D ++Y+D NNL GWA+SQ LP NF WV ++ ++ N + Y +E+D+ PD LH+ +D P AP+ PP+ ++LM T W KK +V+++R L +K G V +HR ++F Q P LK I + ++L + E + N + M N+ +KR+ +R C++ +K +KP F + L+A H+ + + ++P I S+LD+SK+ +Y + KY E L +++ +DTDSF + + + I + DTS+Y +P Sbjct: 116 SLILR-KGVFPYDYIVDHSKF-SERSLPPKECFFNKLNEEPITDDEYAYACEIWNTFNFTTLGDYSDHYLKTDVLLLV-DIFQNFRKVCMGTYSLDPAWYYTSPGLSWDAMLKITKIEIEIFQDYDMLLFVEKGTRGGISQCSKRYARANNKFMIDYDSSKPDNYIMYLDANNLYGWAMSQPLPLKNFKWVDPQTVDI-TQISDDNSQ----GYILEVDLLYPDTLHDAHSDLPFAPETALPPDCTEKRLMTTFWEKKGYVIHYRNLKQCLKFGLEVKKVHRVLQFDQAPFLKPYIDLNTQKRSLSKNDFEKSFYKLMNNSIFGKTMENI----RKRVDVRLCSNGKKAEKLIAKPNFHDRTIFSENLVAFHMQKTHLVFNKPIAIGMSILDISKILMYSFHYENMKPKYGENL----HLLYMDTDSFVYSIETSNIYEDIKNDISH--YDTSDYPANNP 557
BLAST of Rho_N domain-containing protein vs. TrEMBL
Match: A0A4Y2PKA1 (Uncharacterized protein OS=Araneus ventricosus OX=182803 GN=AVEN_114505_1 PE=4 SV=1) HSP 1 Score: 189.504 bits (480), Expect = 7.776e-49 Identity = 131/457 (28.67%), Postives = 236/457 (51.64%), Query Frame = 2 Query: 317 IVRAKGIYPYDFVTSLKVLESNYELPPIW--------SSVSDDEYATAQEI*TLQNCRTM*DYTLVYLK*DLRIIT------KEIAYN--GLEPLNFYGIPGMSMASALMTLRQPIEVLQDM*MYDFYEGGIRGGITFV--------NKY---YAASSEDTELLYIDINNLNGWALSQNLPCDNFVWVHENLNNVLSECMSGNIELLPYSYTIEIDIDIPDRLHNFLNDFPIAPQRICPPNSKVEKLMLTHWPKKNHVLYWRLLNLYIKLGAIVVIIHRAIKFKQTPILKT*IKI-----QICAQTLHVSCIEICTNYLIIVYMVNL*GIGKKRMILRPCNSAEKMIVYDSKPTFRKSIKIDDYLIAAHLNRGSICLDRPNYIDQSVLDLSKLRIYHLQ*NELA-KYREELNCDINIVALDTDSFFLEVLNCKLDTILPTIIRNKLLDTSNYDPVHP 1588 ++ KG+YPYD++ + E ELPPI S++ DD+Y AQ + N + + +Y+ +Y+K D+ I+ +++ L+P ++ PG+S + L + ++++ D+ M E GIRGGI+ NKY Y + E L+Y+D NNL GW +SQ LP F W++ N+ N+L +C + + Y +E+D++ P LH++ D P+AP++ P SK EKL+ T + K N+V+++++L Y+++G + +HR ++F+Q+ LK I + + ++ N + + N+ +KR+ ++ C S ++ Y +KP F+ + L+A H+N+ I LD+P Y+ S+LDLSK + N + KY+ D+ ++ +DTDS ++ + +I DTS+Y +P Sbjct: 193 LITRKGVYPYDYMDCEEKYEET-ELPPIKDFYNRLNDSNIKDDDYKHAQNVWKSFNIKNLREYSELYVKTDVLILADIFEKFRDVCLKTYKLDPAWYFTAPGLSWDAMLKKTQIKLDLIHDIDMVLMIEKGIRGGISQCCNRYAKANNKYMKEYDKNKESNYLMYLDANNLYGWTMSQYLPHGGFKWIYPNIGNIL-KCPDDSKK----GYILEVDLEYPKELHDYHTDLPLAPEKKIPDGSKQEKLLTTLYDKTNYVVHYKILKQYLEMGLKLKKVHRILEFEQSDWLKKYIDLNTEMRKKATNEFEKDFYKLMNNSVFGKAIENV----RKRIDIKFCTSYKQAKRYIAKPNFKGRTIFSENLVAIHMNKTEIKLDKPIYVGMSILDLSKHLMCDFHYNVMKPKYK-----DLTLLYMDTDSLTYDIKTDDFYEDMKNMIN--YFDTSDYQENNP 632
BLAST of Rho_N domain-containing protein vs. TrEMBL
Match: D7GXL4 (Rho_N domain-containing protein OS=Tribolium castaneum OX=7070 GN=TcasGA2_TC006914 PE=4 SV=1) HSP 1 Score: 191.815 bits (486), Expect = 1.988e-48 Identity = 145/457 (31.73%), Postives = 236/457 (51.64%), Query Frame = 2 Query: 317 IVRAKGIYPYDFVTSL-KVLESNYELPPI---WSS-----VSDDEYATAQEI*TLQNCRTM*DY------------TLVYLK*DLRIITKEIAYNGLEPLNFYGIPGMSMASALMTLRQPIEVLQDM*MYDFYEGGIRGGITFVNKYYAASSEDTELLYIDINNLNGWALSQNLPCDNFVWVH----ENLNNVL-SECMSGNIELLPYSYTIEIDIDIPDRL--HNFLNDFPIAPQRICPPNSKVEKLMLTHWPKKNHVLYWRLLNLYIKLGAIVVIIHRAIKFKQTPILKT*IKIQICAQTLHVSCIEICTNYLII--VYMVNL*GIGKKRMILRPCNSAEKMIVYDSKPTFRKSIKIDDYLIAAHLNRGSICLDRPNYIDQSVLDLSKLRIYHLQ*NELAKYREELNCDINIVALDTDSFFLEVLNCKLDTILPTIIRNKLLDTSNYDPVHPLYS 1597 I+R KGIYPY+++ S K E+ +LP I +SS +S +E+ A ++ + + ++ T V+ + R + E +Y GL+P+ +Y PG++ L +E+L D MY F+E GIRGGI+ + Y+ + E+T+++YID NNL GWALSQ LP +F W+ +N++ +L S+ +G Y +E+D+ P L HN ND P+AP+ PP KVEKL+ + KKN++++++ L LY LG + +HR IKF Q+ + +K + + E L++ Y ++ + ++ I C+ EK+ +KP F+ +L A H + + ++P YI Q+VLDLSKL +Y+ N + K E N ++ ++ DTDSF E+ N + DTS+Y H LYS Sbjct: 772 IIR-KGIYPYEYMDSFEKFFET--KLPSIDKFYSSLTKEGISQEEFEHAVKVWKHFKIKNLGEWHDLYLKLDVLLLTDVFE--NFRDLCLEKSY-GLDPVWYYTTPGLAWDCMLKYTSVELELLTDYEMYLFFEKGIRGGISQCSNRYSQADENTQIIYIDANNLYGWALSQKLPEKDFKWLTCDEIKNVDGILVSDSDTG--------YVLEVDLAFPVDLAIHNKFNDLPLAPENRIPPGGKVEKLLCHFYDKKNYIIHYKTLKLYKSLGIRITKVHRGIKFTQSNFIAPYVKFNTEQRQKAKNDFEKDFWKLMVNAPYGKSMQSVRNQQNIKLYCDE-EKLQKAINKPNFKHRTIFSKHLAAVHNFKTKVLFNKPIYIGQAVLDLSKLLMYNFHYNVIKK---EFNQNVKLLYGDTDSFIYEIKNADFYEFMKN--NPDYFDTSDYPKDHSLYS 1208
BLAST of Rho_N domain-containing protein vs. TrEMBL
Match: J9M431 (Uncharacterized protein OS=Acyrthosiphon pisum OX=7029 PE=4 SV=2) HSP 1 Score: 189.504 bits (480), Expect = 1.186e-47 Identity = 147/492 (29.88%), Postives = 237/492 (48.17%), Query Frame = 2 Query: 248 SKLAKAVNT--LTDLPLTCSVFDGI---IVRAKGIYPYDFVTSLKVLESNYELPP--------IWSSVSDDEYATAQEI*TLQNCRTM*DYTLVYLK*DLRIITKEI---------AYNGLEPLNFYGIPGMSMASALMTLRQPIEVLQDM*MYDFYEGGIRGGITFVNKYYAA-----------SSEDTELLYIDINNLNGWALSQNLPCDNFVWVHENLNNVLSECMSGNIELLPYSYTIEIDIDIPDRLHNFLNDFPIAPQRICPPNSKVEKLMLTHWPKKNHVLYWRLLNLYIKLGAIVVIIHRAIKFKQTPILKT*IKIQI-----CAQTLHVSCIEICTNYLIIVYMVNL*GIGKKRMILRPCNSAEKMIVYDSKPTFRKSIKIDDYLIAAHLNRGSICLDRPNYIDQSVLDLSKLRIYHLQ*NELAKYREELNCDINIVALDTDSFFLEVLNCKLDTILPTIIRN----KLLDTSNYDPVHPLYS 1597 S LA+ + T L + T VFD + +V KG+YPY++ S + L+ LP S + ++E+ A+E+ + NC+T+ +Y+ +YLK D+ ++ AYN L+P +++ PG+S + L Q +++L D M +E GIRGG+ V+K YA S E + ++Y D NNL GWA+SQ++P F WV LN + ++ P ++D+ P LH+ ND P PQ P SKV KLM T KKN+++++R L IK G IV +HR I+F Q+ L IK+ ++ N + M + +K M + + ++ +K TF+ I+ +D L A L I D+P YI +VLD+SK +Y N + ++ + I ++ DTDS V + + + +I N LDT++ P+HP Y+ Sbjct: 724 STLAENLMTPGLENFRETARVFDEVDMPLVTRKGVYPYEYTDSWERLKDT-SLPSKRSFYSMLTESRIKENEFDHAKEVWSHFNCKTLGEYSDLYLKIDVLLLADVFENFRDVCMKAYN-LDPAHYFTAPGLSFDAMLKFTGQKLQLLDDYDMLLMFENGIRGGLVQVSKRYAKANNMKTPGYDESKEKSWIVYQDCNNLYGWAMSQSMPYGGFNWVEPTLNGLYDLDVTS-----PIGRVYDVDVSYPHHLHDQHNDLPFLPQNSVPRGSKVRKLMGTFEKKKNYIVHYRSLQQAIKNGLIVEKVHRVIQFNQSDWLAKYIKLNTEMRKRAKNDFEKDFFKLMNNAVFGKTMQS----KRKEMKMELVSCERRLQKLINKTTFKHCIRYNDNLNAVELENKIISFDKPIYIGFAVLDISKTLMYDYHYNVMQRHYGD---KIELMYTDTDSL---VYHVQTEDFYADLIENPNLLDRLDTADLPPIHPCYT 1198
BLAST of Rho_N domain-containing protein vs. TrEMBL
Match: K7JB67 (Uncharacterized protein OS=Nasonia vitripennis OX=7425 PE=4 SV=1) HSP 1 Score: 187.963 bits (476), Expect = 4.191e-47 Identity = 151/490 (30.82%), Postives = 243/490 (49.59%), Query Frame = 2 Query: 248 SKLAKAVNTLTDLPLTCSVF--DGI------IVRAKGIYPYDFVTSLKVLESNYELPP---IWSS-----VSDDEYATAQEI*TLQNCRTM*DYTLVYLK*DLRIITKEIAYN---------GLEPLNFYGIPGMSMASALMTLRQPIEVLQDM*MYDFYEGGIRGGIT------------FVNKYYAASSEDTELLYIDINNLNGWALSQNLPCDNFVWVHE----NLNNVLSECMSGNIELLPYSYTIEIDIDIPDRLHNFLNDFPIAPQRICPPNSKVEKLMLTHWPKKNHVLYWRLLNLYIKLGAIVVIIHRAIKFKQTPILKT*IKI-----QICAQTLHVSCIEICTNYLIIVYMVNL*GIGKKRMILR--PCNSAEKMIVYDSKPTFRKSIKIDDYLIAAHLNRGSICLDRPNYIDQSVLDLSKLRIYHLQ*NELAKYREELNCDINIVALDTDSFFLEVLNCKLDTILPTIIRNKLLDTSNY 1573 S L K + LT+L + VF DG +++ KG++PYD+V+SL L + LP +SS +SD++Y A+ + N +T+ Y+ +YLK D+ I+ E+ N GL+P ++Y PG+S + L + +++L D+ M F E GIRGGI+ ++N+ Y A++++ L+Y D NNL GWA+++ LP F WV + N NV + P Y +E+D++ P LH D P + +CPP SK +KLM T + KKN+V+++R L + G + IHRA++F+Q+ LK I + + ++ N + M N + +++ + AE +I +KPTF D+YL+A + R +C+ +P YI SVLDLSK IY + + E ++ DTDS EV N + ++ I DTS+Y Sbjct: 561 SSLDKLSSYLTELGMLERVFQEDGFTPEQITLLKRKGVFPYDYVSSLAKL-NEVNLPTKEDFFSSLYDAHISDEDYEHAKRVWQELNVQTLGQYSDLYLKTDV-ILLAEVFENFRSNCLQAYGLDPAHYYTTPGLSWDAMLKYTQVRLDLLTDIDMLMFVERGIRGGISQCCNRYAKANNPYMNEGYDANADEKYLVYYDANNLYGWAMTEALPSGKFKWVADLQTPNFFNVPDDG--------PKGYILEVDLEYPIMLHGAHRDMPFCAEHMCPPGSKQKKLMTTLYDKKNYVIHYRALKQALAHGLRLKKIHRALEFEQSKWLKPYIDLNSDMRKNAKNEFEKMLFKLFNNAVYGKTMENERKHVEVKLVTKWEGRYGAEALI---AKPTFHNCTIFDEYLVAVQMFRAEVCIKKPIYIGLSVLDLSKTLIYRFHYDYML---ERAGDKCKLLYTDTDSLVYEVSNLNVYEMMRIDIHE--FDTSDY 1032
BLAST of Rho_N domain-containing protein vs. Ensembl Nematostella
Match: EDO46109 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RR56]) HSP 1 Score: 141.739 bits (356), Expect = 1.693e-35 Identity = 120/452 (26.55%), Postives = 214/452 (47.35%), Query Frame = 2 Query: 329 KGIYPYDFVTSLKVLESNYELPP---IWSS-----VSDDEYATAQEI*TLQNCRTM*DYTLVYLK*DLRIIT------KEIAYN--GLEPLNFYGIPGMSMASALMTLRQPIEVLQDM*MYDFYEGGIRGGITFVNKYYAASSE------DTEL-----LYIDINNLNGWALSQNLPCDNFVWVHENLNNVLSECMSGNIELLPYSYTIEIDIDIPDRLHNFLNDFPIAPQRICPPNSKVEKLMLTHWPKKNHVLYWRLLNLYIKLGAIVVIIHRAIKFKQTPILKT*IKIQICAQTLHVSCIE-----ICTNYLIIVYMVNL*GIGKKRMILRPCNSAEKMIVYDSKPTFRKSIKIDDYLIAAHLNRGSICLDRPNYIDQSVLDLSKLRIYHLQ*NELAKYREELNCDINIVALDTDSFFLEVLNCKLDTILPTIIRNKLLDTSNYDPVHP 1588 KG++PYD+ + L++ +LPP +S+ +S+++Y AQ++ TM DY +YL+ D+ ++ +++ GL+P +Y PG++ +AL R +E+L D M +E GIRGG++ ++ ++ ++ D L Y+D NNL GWA+S+ LP F W+ + L E + +E+D+ P LH+ +D+P+AP+ I +KV KL+ K +V++ L LY LG V +HR I F+++ LKT I + + + E + N + M N+ + R+ +R + ++ SKP ++ + L A H+ + + ++P Y+ +LDLSK +Y + + ++ DTDS E+ + +R+ + DTSNY HP Sbjct: 1063 KGVFPYDWFCNFDQLDA-IQLPPKEAFYSTLNDTDISEEDYQHAQKVWETFKMSTMRDYHDLYLESDVLLLADVFENFRDVCLENYGLDPAWYYTAPGLAWDAALKITRVELELLTDCDMLLMFEEGIRGGVSMISTRHSKANNPYMREYDPNLPTKYITYLDANNLYGWAMSKPLPTHGFRWM---TDQELKEWGR-------HPCVVEVDMAYPRHLHDSHDDYPLAPESI--KINKVGKLIPNLNDKTKYVVHHETLKLYESLGLKVTKVHRGITFEESGWLKTYIDLNTSLRAKATNDFEKDFFKLMNNSVFGKTMENI----RNRVDIRLITNEKQARKLISKPNYKHRTIFCENLAAIHMRKTRLVFNKPVYLGMCILDLSKNLMYDFH---YGYVKPKYGDKAKLLFTDTDSLMYEIETEDFYKDISGDVRS-MFDTSNYPRNHP 1493
BLAST of Rho_N domain-containing protein vs. Planmine SMEST
Match: SMESG000066165.1 (SMESG000066165.1) HSP 1 Score: 656.366 bits (1692), Expect = 0.000e+0 Identity = 330/466 (70.82%), Postives = 378/466 (81.12%), Query Frame = 2 Query: 254 LAKAVNTLTDLPLTCSVFDGIIVRAKGIYPYDFVTSLKVLESNYELPPIWSSVSDDEYATAQEI*TLQNCRTM*DYTLVYLK*DLRIIT--------KEIAYNGLEPLNFYGIPGMSMASALMTLRQPIEVLQDM*MYDFYEGGIRGGITFVNKYYAASSEDTELLYIDINNLNGWALSQNLPCDNFVWVHENLNNVLSECMSGNIELLPYSYTIEIDIDIPDRLHNFLNDFPIAPQRICPPNSKVEKLMLTHWPKKNHVLYWRLLNLYIKLGAIVVIIHRAIKFKQTPILKT*IKIQICAQT-----LHVSCIEICTNYLIIVYMVNL*GIGKKRMILRPCNSAEKMIVYDSKPTFRKSIKIDDYLIAAHLNRGSICLDRPNYIDQSVLDLSKLRIYHLQ*NELAKYREELNCDINIVALDTDSFFLEVLNCKLDTILPTIIRNKLLDTSNYDPVHPLYSRNLDS 1612 LAKAV TLTDLPLTCS FDG+IV+AKGI+PYDF SLKVLES YELPP W SVSDDEYATAQ I T QNC TM DY L YLK D+ ++ K IAYNGLEPLNFYGIPGMS ASALMTL++PIE+LQDM M++FYEGGIRGG+TFVNK++ ASSEDTELLYIDINNL GWALSQNLP +FVWVH+NLN+VLSEC++ NIE LPY YT+E+DI+IP+R+H +LNDFP+AP+++CPPNSKVEKLMLTH+ KKNHVL+WRLL LYI+LGA V IHRAIKFKQ+PI K I+ ++ L+ ++ N L + NL KKRM LR CNSAEKMIVY SKPTF+KSIKI D LIAAHLN+ SICLDRP+YI QSVLDLSKLR+Y LQ NELAKYR E NCDINIVA DTDSFFLEV +CKLD +LP +IR+ LLDTSNYDP HPLYSR LDS Sbjct: 505 LAKAVKTLTDLPLTCSAFDGLIVKAKGIFPYDFAVSLKVLESTYELPPKWDSVSDDEYATAQLIWTQQNCTTMLDYMLTYLKLDVFLLADYFQQFRAKAIAYNGLEPLNFYGIPGMSWASALMTLKEPIELLQDMEMFNFYEGGIRGGLTFVNKHHVASSEDTELLYIDINNLYGWALSQNLPYGDFVWVHDNLNDVLSECVNANIEFLPYGYTMEVDINIPERVHQYLNDFPLAPEKMCPPNSKVEKLMLTHYSKKNHVLHWRLLKLYIELGAEVECIHRAIKFKQSPIFKEYIQRNTDLRSNTKCELYRDLYKLFNNSLYGKSVENL----KKRMNLRLCNSAEKMIVYASKPTFKKSIKIVDDLIAAHLNKDSICLDRPSYIGQSVLDLSKLRMYQLQYNELAKYRSEFNCDINIVAGDTDSFFLEVKHCKLDILLPAMIRDNLLDTSNYDPAHPLYSRKLDS 966
BLAST of Rho_N domain-containing protein vs. Planmine SMEST
Match: SMESG000066165.1 (SMESG000066165.1) HSP 1 Score: 655.981 bits (1691), Expect = 0.000e+0 Identity = 330/466 (70.82%), Postives = 378/466 (81.12%), Query Frame = 2 Query: 254 LAKAVNTLTDLPLTCSVFDGIIVRAKGIYPYDFVTSLKVLESNYELPPIWSSVSDDEYATAQEI*TLQNCRTM*DYTLVYLK*DLRIIT--------KEIAYNGLEPLNFYGIPGMSMASALMTLRQPIEVLQDM*MYDFYEGGIRGGITFVNKYYAASSEDTELLYIDINNLNGWALSQNLPCDNFVWVHENLNNVLSECMSGNIELLPYSYTIEIDIDIPDRLHNFLNDFPIAPQRICPPNSKVEKLMLTHWPKKNHVLYWRLLNLYIKLGAIVVIIHRAIKFKQTPILKT*IKIQICAQT-----LHVSCIEICTNYLIIVYMVNL*GIGKKRMILRPCNSAEKMIVYDSKPTFRKSIKIDDYLIAAHLNRGSICLDRPNYIDQSVLDLSKLRIYHLQ*NELAKYREELNCDINIVALDTDSFFLEVLNCKLDTILPTIIRNKLLDTSNYDPVHPLYSRNLDS 1612 LAKAV TLTDLPLTCS FDG+IV+AKGI+PYDF SLKVLES YELPP W SVSDDEYATAQ I T QNC TM DY L YLK D+ ++ K IAYNGLEPLNFYGIPGMS ASALMTL++PIE+LQDM M++FYEGGIRGG+TFVNK++ ASSEDTELLYIDINNL GWALSQNLP +FVWVH+NLN+VLSEC++ NIE LPY YT+E+DI+IP+R+H +LNDFP+AP+++CPPNSKVEKLMLTH+ KKNHVL+WRLL LYI+LGA V IHRAIKFKQ+PI K I+ ++ L+ ++ N L + NL KKRM LR CNSAEKMIVY SKPTF+KSIKI D LIAAHLN+ SICLDRP+YI QSVLDLSKLR+Y LQ NELAKYR E NCDINIVA DTDSFFLEV +CKLD +LP +IR+ LLDTSNYDP HPLYSR LDS Sbjct: 529 LAKAVKTLTDLPLTCSAFDGLIVKAKGIFPYDFAVSLKVLESTYELPPKWDSVSDDEYATAQLIWTQQNCTTMLDYMLTYLKLDVFLLADYFQQFRAKAIAYNGLEPLNFYGIPGMSWASALMTLKEPIELLQDMEMFNFYEGGIRGGLTFVNKHHVASSEDTELLYIDINNLYGWALSQNLPYGDFVWVHDNLNDVLSECVNANIEFLPYGYTMEVDINIPERVHQYLNDFPLAPEKMCPPNSKVEKLMLTHYSKKNHVLHWRLLKLYIELGAEVECIHRAIKFKQSPIFKEYIQRNTDLRSNTKCELYRDLYKLFNNSLYGKSVENL----KKRMNLRLCNSAEKMIVYASKPTFKKSIKIVDDLIAAHLNKDSICLDRPSYIGQSVLDLSKLRMYQLQYNELAKYRSEFNCDINIVAGDTDSFFLEVKHCKLDILLPAMIRDNLLDTSNYDPAHPLYSRKLDS 990
BLAST of Rho_N domain-containing protein vs. Planmine SMEST
Match: SMESG000066165.1 (SMESG000066165.1) HSP 1 Score: 655.21 bits (1689), Expect = 0.000e+0 Identity = 330/466 (70.82%), Postives = 378/466 (81.12%), Query Frame = 2 Query: 254 LAKAVNTLTDLPLTCSVFDGIIVRAKGIYPYDFVTSLKVLESNYELPPIWSSVSDDEYATAQEI*TLQNCRTM*DYTLVYLK*DLRIIT--------KEIAYNGLEPLNFYGIPGMSMASALMTLRQPIEVLQDM*MYDFYEGGIRGGITFVNKYYAASSEDTELLYIDINNLNGWALSQNLPCDNFVWVHENLNNVLSECMSGNIELLPYSYTIEIDIDIPDRLHNFLNDFPIAPQRICPPNSKVEKLMLTHWPKKNHVLYWRLLNLYIKLGAIVVIIHRAIKFKQTPILKT*IKIQICAQT-----LHVSCIEICTNYLIIVYMVNL*GIGKKRMILRPCNSAEKMIVYDSKPTFRKSIKIDDYLIAAHLNRGSICLDRPNYIDQSVLDLSKLRIYHLQ*NELAKYREELNCDINIVALDTDSFFLEVLNCKLDTILPTIIRNKLLDTSNYDPVHPLYSRNLDS 1612 LAKAV TLTDLPLTCS FDG+IV+AKGI+PYDF SLKVLES YELPP W SVSDDEYATAQ I T QNC TM DY L YLK D+ ++ K IAYNGLEPLNFYGIPGMS ASALMTL++PIE+LQDM M++FYEGGIRGG+TFVNK++ ASSEDTELLYIDINNL GWALSQNLP +FVWVH+NLN+VLSEC++ NIE LPY YT+E+DI+IP+R+H +LNDFP+AP+++CPPNSKVEKLMLTH+ KKNHVL+WRLL LYI+LGA V IHRAIKFKQ+PI K I+ ++ L+ ++ N L + NL KKRM LR CNSAEKMIVY SKPTF+KSIKI D LIAAHLN+ SICLDRP+YI QSVLDLSKLR+Y LQ NELAKYR E NCDINIVA DTDSFFLEV +CKLD +LP +IR+ LLDTSNYDP HPLYSR LDS Sbjct: 567 LAKAVKTLTDLPLTCSAFDGLIVKAKGIFPYDFAVSLKVLESTYELPPKWDSVSDDEYATAQLIWTQQNCTTMLDYMLTYLKLDVFLLADYFQQFRAKAIAYNGLEPLNFYGIPGMSWASALMTLKEPIELLQDMEMFNFYEGGIRGGLTFVNKHHVASSEDTELLYIDINNLYGWALSQNLPYGDFVWVHDNLNDVLSECVNANIEFLPYGYTMEVDINIPERVHQYLNDFPLAPEKMCPPNSKVEKLMLTHYSKKNHVLHWRLLKLYIELGAEVECIHRAIKFKQSPIFKEYIQRNTDLRSNTKCELYRDLYKLFNNSLYGKSVENL----KKRMNLRLCNSAEKMIVYASKPTFKKSIKIVDDLIAAHLNKDSICLDRPSYIGQSVLDLSKLRMYQLQYNELAKYRSEFNCDINIVAGDTDSFFLEVKHCKLDILLPAMIRDNLLDTSNYDPAHPLYSRKLDS 1028
BLAST of Rho_N domain-containing protein vs. Planmine SMEST
Match: SMESG000037212.1 (SMESG000037212.1) HSP 1 Score: 632.098 bits (1629), Expect = 0.000e+0 Identity = 322/466 (69.10%), Postives = 370/466 (79.40%), Query Frame = 2 Query: 254 LAKAVNTLTDLPLTCSVFDGIIVRAKGIYPYDFVTSLKVLESNYELPPIWSSVSDDEYATAQEI*TLQNCRTM*DYTLVYLK*DLRIIT--------KEIAYNGLEPLNFYGIPGMSMASALMTLRQPIEVLQDM*MYDFYEGGIRGGITFVNKYYAASSEDTELLYIDINNLNGWALSQNLPCDNFVWVHENLNNVLSECMSGNIELLPYSYTIEIDIDIPDRLHNFLNDFPIAPQRICPPNSKVEKLMLTHWPKKNHVLYWRLLNLYIKLGAIVVIIHRAIKFKQTPILKT*IKIQICAQT-----LHVSCIEICTNYLIIVYMVNL*GIGKKRMILRPCNSAEKMIVYDSKPTFRKSIKIDDYLIAAHLNRGSICLDRPNYIDQSVLDLSKLRIYHLQ*NELAKYREELNCDINIVALDTDSFFLEVLNCKLDTILPTIIRNKLLDTSNYDPVHPLYSRNLDS 1612 LAKAV TLTDLPLTCS FDG+IV+AKGI+PYDF SL+VLES YELPP W SVSDDEYATAQ I T QNC TM DY L YLK D+ ++ K IAYNGLEPLNFYGIPGMS ASALMTL++PIE+LQDM M++FYEGGIRGG+TFVNK++ ASSEDTELL+IDINNL GWALSQNLP +FVWVH NLN+VLSEC++ NIE LPY YT+E+DI+IP+R+H +LNDFP+AP+++CPPNSKVEKLMLTH+ KKNHVL+W+LL LYI+LGA V IHRAIKFKQ PI K I+ ++ LH ++ N L + NL KKRM LR CNSAEKMIVY SKPTF+KSIKI D LIAAHLN+ SICLDR +YI QSVLDLSKLR+Y LQ NELAKYR E NCDINIVA DTDSFFLEV +CKLD +LP +IR+ LLDT HPLYSR LDS Sbjct: 1122 LAKAVKTLTDLPLTCSAFDGLIVKAKGIFPYDFAVSLEVLESTYELPPKWDSVSDDEYATAQLIWTQQNCTTMLDYMLTYLKLDVFLLADYFQQFRAKAIAYNGLEPLNFYGIPGMSWASALMTLKEPIELLQDMEMFNFYEGGIRGGLTFVNKHHVASSEDTELLHIDINNLYGWALSQNLPYGDFVWVHYNLNDVLSECVNANIEFLPYGYTMEVDINIPERVHQYLNDFPLAPEKMCPPNSKVEKLMLTHYSKKNHVLHWKLLKLYIELGAEVECIHRAIKFKQAPIFKEYIQRNTDLRSNSKCELHRDLYKLFNNSLYGKSVENL----KKRMNLRLCNSAEKMIVYASKPTFKKSIKIVDDLIAAHLNKDSICLDRLSYIGQSVLDLSKLRMYQLQYNELAKYRSEFNCDINIVAGDTDSFFLEVKHCKLDILLPAMIRDSLLDT------HPLYSRKLDS 1577 HSP 2 Score: 122.094 bits (305), Expect = 9.691e-29 Identity = 59/76 (77.63%), Postives = 66/76 (86.84%), Query Frame = 3 Query: 3 KEQIFGDKRMPNFILVTFQSKTQYNGSYTASSSIFKHFNVASLSLSKNTDYSKTYFQDF*NDNYGTTYMQSIVRNM 230 KEQIF D+RMPNFILVTFQS++QYNGSY SSSIFK+F V SL+LSKN+DY +TY QDF NDNY TYMQSIVRNM Sbjct: 365 KEQIFRDRRMPNFILVTFQSESQYNGSYLTSSSIFKNFGVTSLTLSKNSDYRETYTQDFDNDNYCATYMQSIVRNM 440
BLAST of Rho_N domain-containing protein vs. Planmine SMEST
Match: SMESG000050587.1 (SMESG000050587.1) HSP 1 Score: 598.586 bits (1542), Expect = 0.000e+0 Identity = 307/461 (66.59%), Postives = 352/461 (76.36%), Query Frame = 2 Query: 254 LAKAVNTLTDLPLTCSVFDGIIVRAKGIYPYDFVTSLKVLESNYELPPIWSSVSDDEYATAQEI*TLQNCRTM*-------DYTLVYLK*DLRIITKEIAYNGLEPLNFYGIPGMSMASALMTLRQPIEVLQDM*MYDFYEGGIRGGITFVNKYYAASSEDTELLYIDINNLNGWALSQNLPCDNFVWVHENLNNVLSECMSGNIELLPYSYTIEIDIDIPDRLHNFLNDFPIAPQRICPPNSKVEKLMLTHWPKKNHVLYWRLLNLYIKLGAIVVIIHRAIKFKQTPILKT*IKIQI-CAQTLHVSCIEICTNYLIIVYMVNL*GIGKKRMILRPCNSAEKMIVYDSKPTFRKSIKIDDYLIAAHLNRGSICLDRPNYIDQSVLDLSKLRIYHLQ*NELAKYREELNCDINIVALDTDSFFLEVLNCKLDTILPTIIRNKLLDTSNYDPVHPLYSRNLDS 1612 LAKAV TLTDLPLTCS FDG+IV+AKGI+PYDF SL+VLES YELPP W SVSDDEYATAQ I T QNC T+ Y L + + T E +N MASALMTL++PIE+LQDM M++FYEGGIRGG+TFVNK++ ASSEDTELLYIDINNL GWALSQNLP +FVWVH+NLN+VLSEC++ NIE LPY YT+E+DI+IP+R+H +LNDFP+AP+++CPPNSKVEKLMLTH+ KKNHVL+WRLL LYI+LGA V IHRA+KFKQ+PI K I+ AQT S IEIC NYLII YM EKMIVY SKPTF+KSIKI D LIAAHLN+ SICLDRP+YI QSVLDLSKLR+Y LQ NELAKYR E NCDINIVA DTDSFFLEV +CKLD +LP +IR+ LLDTSNYDP HPLYSR LDS Sbjct: 706 LAKAVKTLTDLPLTCSAFDGLIVKAKGIFPYDFAVSLEVLESTYELPPKWDSVSDDEYATAQLIWTQQNCTTILFSAISRKGYCLQWFR------TSEFLWNSWN----------VMASALMTLKEPIELLQDMEMFNFYEGGIRGGLTFVNKHHVASSEDTELLYIDINNLYGWALSQNLPYGDFVWVHDNLNDVLSECVNANIEFLPYGYTMEVDINIPERVHQYLNDFPLAPEKMCPPNSKVEKLMLTHYSKKNHVLHWRLLKLYIELGAEVECIHRAMKFKQSPIFKEYIQRNTDYAQTQSASFIEICINYLIIAYM-----------------GTEKMIVYASKPTFKKSIKIVDDLIAAHLNKDSICLDRPSYIGQSVLDLSKLRMYQLQYNELAKYRSEFNCDINIVAGDTDSFFLEVKHCKLDILLPAMIRDNLLDTSNYDPAHPLYSRKLDS 1133 HSP 2 Score: 123.25 bits (308), Expect = 4.326e-29 Identity = 58/76 (76.32%), Postives = 67/76 (88.16%), Query Frame = 3 Query: 3 KEQIFGDKRMPNFILVTFQSKTQYNGSYTASSSIFKHFNVASLSLSKNTDYSKTYFQDF*NDNYGTTYMQSIVRNM 230 KEQIFGD+RMPNFILVTFQS++QYNGSY S+SIFK+F V SL+LSKN+DY +TY QDF NDNY +YMQSIVRNM Sbjct: 251 KEQIFGDRRMPNFILVTFQSESQYNGSYLTSNSIFKNFGVTSLTLSKNSDYRETYTQDFENDNYCASYMQSIVRNM 326 The following BLAST results are available for this feature:
BLAST of Rho_N domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of Rho_N domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 1
BLAST of Rho_N domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of Rho_N domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of Rho_N domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of Rho_N domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of Rho_N domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of Rho_N domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Rho_N domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of Rho_N domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of Rho_N domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of Rho_N domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 1
BLAST of Rho_N domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of Rho_N domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30029260 ID=SMED30029260|Name=Rho_N domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1613bpback to top protein sequence of SMED30029260-orf-1 >SMED30029260-orf-1 ID=SMED30029260-orf-1|Name=SMED30029260-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=158bp MYDFYEGGIRGGITFVNKYYAASSEDTELLYIDINNLNGWALSQNLPCDNback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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