DDE_3 domain-containing protein

Overview
NameDDE_3 domain-containing protein
Smed IDSMED30029253
Length (bp)1229
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of DDE_3 domain-containing protein (SMED30029253) t-SNE clustered cells

Violin plots show distribution of expression levels for DDE_3 domain-containing protein (SMED30029253) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of DDE_3 domain-containing protein (SMED30029253) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for DDE_3 domain-containing protein (SMED30029253) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Homology
[Close]
BLAST of DDE_3 domain-containing protein vs. TrEMBL
Match: A0A0C2JI12 (DDE_3 domain-containing protein OS=Thelohanellus kitauei OX=669202 GN=RF11_01522 PE=4 SV=1)

HSP 1 Score: 199.134 bits (505), Expect = 1.169e-74
Identity = 93/207 (44.93%), Postives = 136/207 (65.70%), Query Frame = 1 
Query:  214 NSTTSNADRDRVITAYDDGTSIVDISRILSIKRTTIYGILKKYHNTGVVEADKRGGDHPQKLTEDQKNQVKEWVDDNCQLSLKVLVEKVFTEFQIRISITYVYNLLCRFHYSMKRLHLIPERRNSTEVIETRKEYALNFSSILSIYSEQELIFIDEVGFNVSMRTTKGRSQIGTPATLNVPTIRSRNISIACAMNKNGLLFYKSFNR 834
            N+  S+ DR R++ AY  G    +IS +L + R+TI  ++K +H +G ++++KRG   P+KL EDQK ++K WVDDN  + L+ +V KV  E  I +  + +  +L RFHYS KR+ +IPERRN+   +  R+ YA  +  +L  + E + IFIDE G N+SMRT+ GRS+IG+PA   VP +RSRNISI  AMN NG+L +K  +R
Sbjct:    3 NNKLSDLDRKRIVDAYQKGQKASEISLVLGVARSTINSVIKIFHQSGRIDSNKRGYIQPEKLNEDQKERIKSWVDDNAGIPLRTIVTKVQEEMDISVGKSTIDRILQRFHYSWKRVSIIPERRNNESTLNIRERYATRYLELLQSFREHQFIFIDECGVNISMRTSHGRSKIGSPAIHVVPNLRSRNISICAAMNVNGMLLHKWHDR 209

HSP 2 Score: 111.694 bits (278), Expect = 1.169e-74
Identity = 54/123 (43.90%), Postives = 72/123 (58.54%), Query Frame = 3 
Query:  834 ALNKEKFIEYITELKEVLIDKEIHRCVFIMDNVKFHKSPEVLSAINNENTTVKFLPPYSPFLNPIENMFSKWKEITKKANPRKDDDLMRAIEIGSTLISSQDCSNYVRNMWSYIPRCIEGEII 1202
            A N++ F  YI EL E +    I     +MDNV FH   E+   I+    T+ FLPPYSPF+NPIENMFSKWK   +       ++L + I   S+LI+S DCS Y  +M+ YI RC+  E+I
Sbjct:  210 AFNRDLFGNYIDELLEKIRALNIEHATIVMDNVSFHHCEEISQQISEAGHTLLFLPPYSPFMNPIENMFSKWKREIRYRRSENSEELYQNITAASSLITSSDCSGYYSHMFRYINRCLRREVI 332
[Close]
BLAST of DDE_3 domain-containing protein vs. TrEMBL
Match: A0A0C2MSM9 (DDE_3 domain-containing protein OS=Thelohanellus kitauei OX=669202 GN=RF11_13861 PE=4 SV=1)

HSP 1 Score: 182.185 bits (461), Expect = 1.430e-68
Identity = 94/229 (41.05%), Postives = 142/229 (62.01%), Query Frame = 1 
Query:  151 VVESDNTAEPAIRNTFRVQRRNSTTSNADRDRVITAYDDGTSIVDISRILSIKRTTIYGILKKYHNTGVVEADKRGGDHPQKLTEDQKNQVKEWVDDNCQLSLKVLVEKVFTEFQIRISITYVYNLLCRFHYSMKRLHLIPERRNSTEVIETRKEYALNFSSILSIYSEQELIFIDEVGFNVSMRTTKGRSQIGTPATLNVPTIRSRNISIACAMNKNGLLFYKSFNRP 837
            V E  NT +P +    R +      S+  R R+I A+ +G+++ ++S   ++ R+TI  I+KKY  TG +  + RGG    K+ E+ K  ++E VD+NC LSLK + + V T F I +S T ++N L + HYS+K +H+IP RRN    I  R  +A +F  +   Y ++++ F+DEVGFNVSMR  +GRS IGT   L VP IRSRN+S+ CAMN+ G+++ +  NRP
Sbjct:   14 VDEGLNTGDPTVP---RGRSSYQKISDERRQRIINAHQNGSTVKNVSEYENLPRSTIQNIVKKYSETGRISKENRGGRTHFKVNEEIKTFIRELVDENCGLSLKQICQNVATRFDITLSETTIHNCLGQMHYSLKAIHIIPTRRNDPNTICIRNHFAHSFLRLEENYPDEQIYFVDEVGFNVSMRIKRGRSSIGTTPILRVPNIRSRNVSVCCAMNRYGVVYKEINNRP 239

HSP 2 Score: 108.227 bits (269), Expect = 1.430e-68
Identity = 54/125 (43.20%), Postives = 72/125 (57.60%), Query Frame = 3 
Query:  840 NKEKFIEYITELKEVL--IDKEIHRCVFIMDNVKFHKSPEVLSAINNENTTVKFLPPYSPFLNPIENMFSKWKEITKKANPRKDDDLMRAIEIGSTLISSQDCSNYVRNMWSYIPRCIEGEIILN 1208
            N E F+ YI    + +  I +   RCV IMDNV+FHKS  ++     +   + +LPPYSPFLNPIE +FSKWK   K A+P  + +L   +  G T I+  DC  Y R+M  Y+ R I  EII N
Sbjct:  241 NTETFLGYIENFFQHIESISQTNTRCVVIMDNVRFHKSSTIVDCFRQKGHEICYLPPYSPFLNPIEELFSKWKRYVKSASPENETELFNCMTQGLTTITRDDCDGYYRHMKRYVRRSINNEIIEN 365
[Close]
BLAST of DDE_3 domain-containing protein vs. TrEMBL
Match: A0A0C2MVP0 (DDE_3 domain-containing protein OS=Thelohanellus kitauei OX=669202 GN=RF11_02952 PE=4 SV=1)

HSP 1 Score: 180.259 bits (456), Expect = 5.305e-68
Identity = 94/229 (41.05%), Postives = 142/229 (62.01%), Query Frame = 1 
Query:  151 VVESDNTAEPAIRNTFRVQRRNSTTSNADRDRVITAYDDGTSIVDISRILSIKRTTIYGILKKYHNTGVVEADKRGGDHPQKLTEDQKNQVKEWVDDNCQLSLKVLVEKVFTEFQIRISITYVYNLLCRFHYSMKRLHLIPERRNSTEVIETRKEYALNFSSILSIYSEQELIFIDEVGFNVSMRTTKGRSQIGTPATLNVPTIRSRNISIACAMNKNGLLFYKSFNRP 837
            V ES NT +P +    R +      S+  R R+I A+ +G+++ ++S   ++ R+TI  I+KK   TG +  + RGG    K+ E+ K  ++E VD+NC LSLK + + V T F I +S T ++N L + HYS+K +H+IP RRN    I  R  +A +F  +   Y ++++ F+DEVGFNVSMR  +GRS IGT   L VP IRSRN+S+ CAMN+ G+++ +  NRP
Sbjct:   16 VDESLNTGDPTVP---RGRSSYQKISDERRQRIINAHQNGSTVKNVSEYENLPRSTIQNIVKKSSETGRISKENRGGRTHFKVNEEIKTFIRELVDENCGLSLKQICQNVATRFDITLSETTIHNCLGQMHYSLKAIHIIPTRRNDPNTICIRNHFAHSFLRLEENYPDEQIYFVDEVGFNVSMRIKRGRSSIGTTPILRVPNIRSRNVSVCCAMNRYGVVYKEINNRP 241

HSP 2 Score: 108.227 bits (269), Expect = 5.305e-68
Identity = 54/125 (43.20%), Postives = 73/125 (58.40%), Query Frame = 3 
Query:  840 NKEKFIEYITELKEVL--IDKEIHRCVFIMDNVKFHKSPEVLSAINNENTTVKFLPPYSPFLNPIENMFSKWKEITKKANPRKDDDLMRAIEIGSTLISSQDCSNYVRNMWSYIPRCIEGEIILN 1208
            N E F+ YI    + +  I +   RCV IMDNV+FHKS  ++     +   + + PPYSPFLNPIE +FSKWK   K A+P  + +L   +  G T I+ +DC  Y R+M SY+ R I  EII N
Sbjct:  243 NTETFLGYIEIFFQHIESISQTNTRCVVIMDNVRFHKSSTIVDCFRQKGHEICYFPPYSPFLNPIEELFSKWKRYVKSASPENETELFNCMTQGLTTITREDCDGYYRHMKSYVRRSINNEIIEN 367
[Close]
BLAST of DDE_3 domain-containing protein vs. TrEMBL
Match: A0A182MSG7 (Paired domain-containing protein OS=Anopheles culicifacies OX=139723 PE=4 SV=1)

HSP 1 Score: 167.162 bits (422), Expect = 5.964e-68
Identity = 91/213 (42.72%), Postives = 138/213 (64.79%), Query Frame = 1 
Query:  220 TTSNADRDRVITAYDDGTSIVDISRILSIKRTTIYGILKKYHNTGVVEADKRGGDHPQKLTEDQKNQVKEWVDDNCQLSLKVLVEKVFTEFQIRISITYVYNLLCRFHYSMKRLHLIPERRNSTEVIETRKEYALNFSSILSIYSEQELIFIDEVGFNVSMRTTKGRSQIGTPATLNVPTIRSRNISIACAMNKNGLLFY----KSFNRPSIK 846
            TTS+ DR R+++AY  GT    ISR+  +  +T+Y ILK Y  T  VEA KRGG++P+ L+ D    +++W++D+C +SLK L +K++ ++QIR S++ +   +  F+Y++KRL  IPE RN+ +++  RKEYA N  ++L  +S+  ++F+ EV F+VSMRT+  RS     A   VP IRSR I I CAMN  G+L Y    ++ NR S K
Sbjct:  611 TTSDEDRQRIVSAYLSGTVPKVISRMFYVNLSTVYNILKNYKKTAHVEAKKRGGNNPKLLSVDAIASIQKWIEDDCTISLKKLADKLYCQYQIRASVSTIAREIKGFNYTIKRLQQIPECRNTEKIVVERKEYAKNLYNLLDQFSDNSMVFVGEVRFHVSMRTSSRRSAPDAAALTVVPNIRSRIIIIICAMNMQGILHYSVHHQAINRESFK 823

HSP 2 Score: 120.939 bits (302), Expect = 5.964e-68
Identity = 54/123 (43.90%), Postives = 79/123 (64.23%), Query Frame = 3 
Query:  834 ALNKEKFIEYITELKEVLIDKEIHRCVFIMDNVKFHKSPEVLSAINNENTTVKFLPPYSPFLNPIENMFSKWKEITKKANPRKDDDLMRAIEIGSTLISSQDCSNYVRNMWSYIPRCIEGEII 1202
            A+N+E F +++ +LK  L    +HR V IMD+  F K  E++ A+++E   V  LP +SPFLNPIEN+F KW  + K  NP+ + DL+  I  G T ++++DC  Y+RNMWSY  RC+  E I
Sbjct:  816 AINRESFKDFVLQLKCELRSNHLHRSVIIMDDTVFDKCAEIIEAMDDEQDHVVHLPQFSPFLNPIENLFGKWVNMVKLMNPQNEKDLIEGIVKGPTTVTAEDCEEYIRNMWSYASRCLRREEI 938

HSP 3 Score: 97.0561 bits (240), Expect = 8.270e-18
Identity = 49/122 (40.16%), Postives = 80/122 (65.57%), Query Frame = 1 
Query:  217 STTSNADRDRVITAYDDGTSIVDISRILSIKRTTIYGILKKYHNTGVVEADKRGGDHPQKLTEDQKNQVKEWVDDNCQLSLKVLVEKVFTEFQIRISITYVYNLLCRFHYSMKRLHLIPERR 582
            +TTS+ DR+R++ AY +G+S   IS +L+IK  T+Y ILKKY +TG V AD RGG+H + L+++  N ++ W++++  LSLK L  KV+  + +R+S T V   +     +++R  LI E +
Sbjct:  288 NTTSDTDRERIVKAYLNGSSGKLISEMLNIKLFTVYNILKKYRDTGKVHADSRGGNHKKLLSKEAGNSIRNWLEEDSTLSLKQLAIKVWETYHLRVSTTTVAREVKALRKTLQRRKLIQEAK 409
[Close]
BLAST of DDE_3 domain-containing protein vs. TrEMBL
Match: A0A4Y0BHL4 (DDE_3 domain-containing protein OS=Anopheles funestus OX=62324 PE=4 SV=1)

HSP 1 Score: 179.874 bits (455), Expect = 1.194e-67
Identity = 90/210 (42.86%), Postives = 136/210 (64.76%), Query Frame = 1 
Query:  205 QRRNSTTSNADRDRVITAYDDGTSIVDISRILSIKRTTIYGILKKYHNTGVVEADKRGGDHPQKLTEDQKNQVKEWVDDNCQLSLKVLVEKVFTEFQIRISITYVYNLLCRFHYSMKRLHLIPERRNSTEVIETRKEYALNFSSILSIYSEQELIFIDEVGFNVSMRTTKGRSQIGTPATLNVPTIRSRNISIACAMNKNGLLFYKSFNR 834
            +RR   TS+ DR RV+ AY+ G ++  I+ +LS+KR T++ ILK Y  TG  EA KRGG   +KLT      ++ W+D++C +SL  L +K++    IR+S   +   +  F+YS KR+ ++PERRN++  +E RKEYAL+++ I   + +  ++FI EV FN++MRT KGR  IG+     V  +R+RNISI CAMN+ G+L Y + NR
Sbjct:  131 KRRCKATSDEDRKRVVAAYEKGCTVRMIAEMLSLKRPTVHSILKSYWKTGEYEARKRGGIKTKKLTSAILVDMQSWMDEDCSISLSKLSQKIYECHAIRVSKPTIARAIKNFNYSFKRVKILPERRNNSSTVEERKEYALSYNRITQRFPQAAIVFIGEVAFNITMRTVKGRPLIGSIDEKVV--LRTRNISIVCAMNRAGILHYITINR 338

HSP 2 Score: 107.457 bits (267), Expect = 1.194e-67
Identity = 50/125 (40.00%), Postives = 80/125 (64.00%), Query Frame = 3 
Query:  837 LNKEKFIEYITELKEVLIDKEI-HRCVFIMDNVKFHKSPEVLSAINNENTTVKFLPPYSPFLNPIENMFSKWKEITKKANPRKDDDLMRAIEIGSTLISSQDCSNYVRNMWSYIPRCIEGEIILN 1208
            +N+E  + +I +LKE L ++++ ++ + I++NV FHK   +L   + E   VK+LPP SPFLNP+E  F+  K + K+ NP+ + ++M AIE G++L + +DC  Y   MW YI RC  GE I +
Sbjct:  340 INQELLVGFIKQLKENLKEQDVGNQTLIIVNNVAFHKCTTILETFSAEGCKVKYLPPNSPFLNPVEMFFNDLKNLIKRTNPQHEQEIMSAIEKGASLTTVKDCEGYFGEMWQYIERCRLGEEITD 464
[Close]
BLAST of DDE_3 domain-containing protein vs. Planmine SMEST
Match: SMESG000059107.1 (SMESG000059107.1)

HSP 1 Score: 334.339 bits (856), Expect = 1.211e-150
Identity = 159/169 (94.08%), Postives = 162/169 (95.86%), Query Frame = 1 
Query:  328 ILKKYHNTGVVEADKRGGDHPQKLTEDQKNQVKEWVDDNCQLSLKVLVEKVFTEFQIRISITYVYNLLCRFHYSMKRLHLIPERRNSTEVIETRKEYALNFSSILSIYSEQELIFIDEVGFNVSMRTTKGRSQIGTPATLNVPTIRSRNISIACAMNKNGLLFYKSFNR 834
            + +KYHNTGVVEADKR GDHPQKLTEDQKNQVKEWVDDNCQLSLKVLVE VFTEFQIRIS TYVYNLL  FHYSMKRLHLIPERRNSTEVIETRKEYALNFS ILSIYSEQELIFIDEVGFNVSMRTT+GRSQIGTPATLNVPTIRSRNISIACAMNKNGLLFYKSFNR
Sbjct: 1277 VTQKYHNTGVVEADKRDGDHPQKLTEDQKNQVKEWVDDNCQLSLKVLVENVFTEFQIRISTTYVYNLLSLFHYSMKRLHLIPERRNSTEVIETRKEYALNFSFILSIYSEQELIFIDEVGFNVSMRTTRGRSQIGTPATLNVPTIRSRNISIACAMNKNGLLFYKSFNR 1445

HSP 2 Score: 219.164 bits (557), Expect = 1.211e-150
Identity = 105/113 (92.92%), Postives = 107/113 (94.69%), Query Frame = 3 
Query:  825 F*SALNKEKFIEYITELKEVLIDKEIHRCVFIMDNVKFHKSPEVLSAINNENTTVKFLPPYSPFLNPIENMFSKWKEITKKANPRKDDDLMRAIEIGSTLISSQDCSNYVRNM 1163
            F  ALNKEKFIEYITELKEVLIDKEIHRCVFIMDN KFHKSPEVLSAINNENTTVKFLPPYSPFLNPIENMFSKWKEI K+AN R +DDLMRAIEIGSTLISSQDCSNYVRNM
Sbjct: 1443 FNRALNKEKFIEYITELKEVLIDKEIHRCVFIMDNAKFHKSPEVLSAINNENTTVKFLPPYSPFLNPIENMFSKWKEIIKRANLRNEDDLMRAIEIGSTLISSQDCSNYVRNM 1555
[Close]
BLAST of DDE_3 domain-containing protein vs. Planmine SMEST
Match: SMESG000026481.1 (SMESG000026481.1)

HSP 1 Score: 135.191 bits (339), Expect = 5.383e-60
Identity = 70/163 (42.94%), Postives = 102/163 (62.58%), Query Frame = 1 
Query:  364 ADKRGGDHPQKLTEDQKNQVKEWVDDNCQLSLKVLVEKVFTEFQIRISITYVYNLLCRFHYSMKRLHLIPERRNSTEVIETRKEYALNFSSILSIYSEQELIFIDEVGFNVSMRTTKGRSQIGTPATLNVPTIRSRNISIACAMNKNGLLFY----KSFNRPS 840
            + K GG   + L+E  K  +K W+D NC L+LK +  K+  E  + +S + +   +  F YSMKR++++P +RN+ EVIE R +YA  F  +LS   + ++ FIDEVGFNVS+R+ +GRS     A  NV +IR+RNIS+ CAMNK G+  Y    KS+N  S
Sbjct:  457 SPKWGGQRRKSLSEQDKEAIKGWIDKNCALTLKQIKSKIELERGVVVSQSTINRCIGGFGYSMKRINIMPIKRNTVEVIELRAQYANRFMELLSTIDDSKIFFIDEVGFNVSIRSRRGRSLKNKRAVQNVSSIRARNISVCCAMNKEGIFKYFTQTKSYNTQS 619

HSP 2 Score: 115.546 bits (288), Expect = 5.383e-60
Identity = 55/121 (45.45%), Postives = 74/121 (61.16%), Query Frame = 3 
Query:  834 ALNKEKFIEYITELKEVLIDKEIHRCVFIMDNVKFHKSPEVLSAINNENTTVKFLPPYSPFLNPIENMFSKWKEITKKANPRKDDDLMRAIEIGSTLISSQDCSNYVRNMWSYIPRCIEGE 1196
            + N + F+E+I  + E +    I   +FIMDNV FHK+  +   I      + FLPPYSPFLNPIEN+F KWK+  + + P  +D L   IE GS+LISS DC NY R+M  ++P CI  E
Sbjct:  614 SYNTQSFLEFIRLIIEKMSSLNISGAIFIMDNVPFHKNIAIKEEIEISGHRILFLPPYSPFLNPIENLFLKWKQFIRSSRPEGEDQLFENIENGSSLISSDDCKNYFRHMLGFLPICIRNE 734
[Close]
BLAST of DDE_3 domain-containing protein vs. Planmine SMEST
Match: SMESG000026481.1 (SMESG000026481.1)

HSP 1 Score: 115.546 bits (288), Expect = 7.929e-49
Identity = 55/121 (45.45%), Postives = 74/121 (61.16%), Query Frame = 3 
Query:  834 ALNKEKFIEYITELKEVLIDKEIHRCVFIMDNVKFHKSPEVLSAINNENTTVKFLPPYSPFLNPIENMFSKWKEITKKANPRKDDDLMRAIEIGSTLISSQDCSNYVRNMWSYIPRCIEGE 1196
            + N + F+E+I  + E +    I   +FIMDNV FHK+  +   I      + FLPPYSPFLNPIEN+F KWK+  + + P  +D L   IE GS+LISS DC NY R+M  ++P CI  E
Sbjct:   96 SYNTQSFLEFIRLIIEKMSSLNISGAIFIMDNVPFHKNIAIKEEIEISGHRILFLPPYSPFLNPIENLFLKWKQFIRSSRPEGEDQLFENIENGSSLISSDDCKNYFRHMLGFLPICIRNE 216

HSP 2 Score: 97.8265 bits (242), Expect = 7.929e-49
Identity = 50/101 (49.50%), Postives = 69/101 (68.32%), Query Frame = 1 
Query:  550 MKRLHLIPERRNSTEVIETRKEYALNFSSILSIYSEQELIFIDEVGFNVSMRTTKGRSQIGTPATLNVPTIRSRNISIACAMNKNGLLFY----KSFNRPS 840
            MKR++++P +RN+ EVIE R +YA  F  +LS   + ++ FIDEVGFNVS+R+ +GRS     A  NV +IR+RNIS+ CAMNK G+  Y    KS+N  S
Sbjct:    1 MKRINIMPIKRNTVEVIELRAQYANRFMELLSTIDDSKIFFIDEVGFNVSIRSRRGRSLKNKRAVQNVSSIRARNISVCCAMNKEGIFKYFTQTKSYNTQS 101
[Close]
BLAST of DDE_3 domain-containing protein vs. Planmine SMEST
Match: SMESG000015851.1 (SMESG000015851.1)

HSP 1 Score: 115.161 bits (287), Expect = 5.041e-31
Identity = 52/104 (50.00%), Postives = 74/104 (71.15%), Query Frame = 1 
Query:  496 IRISITYVYNLLCRFHYSMKRLHLIPERRNSTEVIETRKEYALNFSSILSIYSEQELIFIDEVGFNVSMRTTKGRSQIGTPATLNVPTIRSRNISIACAMNKNG 807
            ++  ++ + N L  F YS+KR+H +P RRN+ + + TRK+YA+NF  + S + E+  IFIDEVGFN++MR+T  RS IG P    VP++RSRNIS+ C MNKNG
Sbjct:   10 LKFGLSTIANALQGFRYSLKRVHFLPIRRNTKDTLITRKDYAMNFICLQSNFVEENFIFIDEVGFNIAMRSTSRRSAIGIPDIKLVPSLRSRNISVCCRMNKNG 113
[Close]
BLAST of DDE_3 domain-containing protein vs. Planmine SMEST
Match: SMESG000003130.1 (SMESG000003130.1)

HSP 1 Score: 104.76 bits (260), Expect = 3.362e-27
Identity = 47/104 (45.19%), Postives = 74/104 (71.15%), Query Frame = 1 
Query:  430 WVDDNCQLSLKVLVEKVFTEFQIRISITYVYNLLCRFHYSMKRLHLIPERRNSTEVIETRKEYALNFSSILSIYSEQELIFIDEVGFNVSMRTTKGRSQIGTPA 741
             VDD+C L+LKV+ ++V+ + +I++ ++ + N L  F YS+KR+H +P RRN+ + + TRK+Y +N   + S ++E+  IFIDEV FNV MR+TK RS +GT A
Sbjct:    1 MVDDDCTLTLKVISQRVYEKHEIKLGLSTIANALQEFRYSLKRVHFLPIRRNTEDTLITRKDYNMNLIRLQSNFAEENYIFIDEVDFNVDMRSTKERSDVGTAA 104
The following BLAST results are available for this feature:
BLAST of DDE_3 domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DDE_3 domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DDE_3 domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DDE_3 domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DDE_3 domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DDE_3 domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DDE_3 domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DDE_3 domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0C2JI121.169e-7444.93DDE_3 domain-containing protein OS=Thelohanellus k... [more]
A0A0C2MSM91.430e-6841.05DDE_3 domain-containing protein OS=Thelohanellus k... [more]
A0A0C2MVP05.305e-6841.05DDE_3 domain-containing protein OS=Thelohanellus k... [more]
A0A182MSG75.964e-6842.72Paired domain-containing protein OS=Anopheles culi... [more]
A0A4Y0BHL41.194e-6742.86DDE_3 domain-containing protein OS=Anopheles funes... [more]
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BLAST of DDE_3 domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DDE_3 domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DDE_3 domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DDE_3 domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DDE_3 domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DDE_3 domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000059107.11.211e-15094.08SMESG000059107.1[more]
SMESG000026481.15.383e-6042.94SMESG000026481.1[more]
SMESG000026481.17.929e-4945.45SMESG000026481.1[more]
SMESG000015851.15.041e-3150.00SMESG000015851.1[more]
SMESG000003130.13.362e-2745.19SMESG000003130.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30029253 ID=SMED30029253|Name=DDE_3 domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1229bp
AACAATATTAAATCTATTATTGACAATATATACTAAATTAATGACAATAA
AAACTTTATGAATGACATTATAAATATATTTTTAGACATTTTATGTATGG
CTCGACAAATAAATCAATTTCCTGCTTCCATAGCAAATATTACAGAAATA
GTAGTCGAAAGTGATAACACCGCCGAACCAGCTATCCGTAACACTTTTCG
AGTACAACGAAGAAATAGTACAACTTCAAATGCTGATAGAGATCGTGTTA
TTACAGCATATGATGACGGGACTTCTATAGTTGATATTTCTCGAATTTTA
TCAATTAAAAGAACTACAATTTATGGTATTTTGAAGAAGTACCATAACAC
GGGAGTAGTGGAAGCTGATAAGAGAGGCGGCGATCATCCACAGAAATTGA
CAGAGGATCAAAAAAATCAAGTAAAAGAGTGGGTTGATGATAATTGCCAA
TTATCGTTGAAAGTTTTGGTGGAAAAGGTATTTACAGAGTTTCAAATTAG
AATATCCATTACTTATGTATATAATTTATTATGTCGTTTTCATTATTCAA
TGAAAAGACTTCATTTAATTCCAGAGAGAAGAAATTCAACTGAAGTAATT
GAAACAAGAAAAGAATATGCACTTAACTTCTCTTCTATACTAAGTATATA
TTCAGAACAAGAATTAATATTTATTGATGAGGTGGGATTCAACGTTTCTA
TGAGAACAACAAAAGGGAGATCACAAATAGGCACTCCTGCAACATTAAAT
GTACCGACCATACGATCAAGAAATATATCTATAGCATGTGCAATGAACAA
AAATGGGCTATTGTTTTATAAATCTTTTAATCGGCCCTCAATAAAGAGAA
GTTTATCGAATATATAACAGAGTTAAAGGAAGTTTTAATTGATAAAGAGA
TTCACAGATGCGTATTCATTATGGATAATGTTAAGTTCCATAAAAGTCCT
GAAGTGCTTAGCGCTATAAATAATGAAAATACAACTGTAAAATTTCTTCC
GCCGTACTCCCCTTTTCTCAATCCTATTGAGAACATGTTCAGCAAGTGGA
AGGAAATCACCAAAAAAGCCAACCCCAGAAAGGATGATGATCTTATGCGA
GCAATTGAAATTGGAAGTACCCTAATATCTTCACAAGACTGTAGTAATTA
TGTGCGAAATATGTGGTCATATATACCAAGATGTATTGAGGGAGAAATTA
TTCTTAATTAAATGTCACTTAATTTTTTT
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protein sequence of SMED30029253-orf-1

>SMED30029253-orf-1 ID=SMED30029253-orf-1|Name=SMED30029253-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=270bp
MNDIINIFLDILCMARQINQFPASIANITEIVVESDNTAEPAIRNTFRVQ
RRNSTTSNADRDRVITAYDDGTSIVDISRILSIKRTTIYGILKKYHNTGV
VEADKRGGDHPQKLTEDQKNQVKEWVDDNCQLSLKVLVEKVFTEFQIRIS
ITYVYNLLCRFHYSMKRLHLIPERRNSTEVIETRKEYALNFSSILSIYSE
QELIFIDEVGFNVSMRTTKGRSQIGTPATLNVPTIRSRNISIACAMNKNG
LLFYKSFNRPSIKRSLSNI*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000074oocyte
PLANA:0000231vitelline gland
Vocabulary: INTERPRO
TermDefinition
IPR009057Homeobox-like_sf
IPR001523Paired_dom
Vocabulary: molecular function
TermDefinition
GO:0003677DNA binding
Vocabulary: biological process
TermDefinition
GO:0006355regulation of transcription, DNA-templated