Serine/threonine-protein kinase NIM1
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30029249 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 8
Homology
BLAST of Serine/threonine-protein kinase NIM1 vs. Ensembl Human
Match: NIM1K (NIM1 serine/threonine protein kinase [Source:HGNC Symbol;Acc:HGNC:28646]) HSP 1 Score: 395.201 bits (1014), Expect = 1.253e-132 Identity = 199/404 (49.26%), Postives = 260/404 (64.36%), Query Frame = 2
Query: 161 TSFDKYTEEMNCNENVKKEIMLGKRVGLYRIKGNLGNGNFSQVKLAVHLLTREEVAIKILDKTKLDAKTQKLLSREISSMERLHHPNIIRLYEVIEDIRKMHLVMEYASGGELHSLIVSHGKIPESISKLIFAQIIAAINHLHSQNVIHRDLKAENIFFAGNKSETNQDNSKLNSTTKKSDFTDNAFSRLVHVKLGDFGFSTLAQPAEKLTTFCGSPPYAAPELFQADWYYGTCVDIWALGILLYFMLCGCMPFRGESVGLLKKLILAGNYVCPNWLPVDAKNLIQQILKQNPDQRLTIQQIKNHQWLSGQHFPDAYQPFKATPL-MPEIETISHAXXXXXXXXXXXXPLEKDSIEASKMLLRLGITDKHIEVAQAQTVRNYVTGTYRIMLHRVHRNRRMSNIP 1369
T F+K T++M+ +E V +EI LGKR+G YRI+G +G+GNFSQVKL +H LT+E+VAIKILDKTKLD KTQ+LLSREISSME+LHHPNIIRLYEV+E + K+HLVMEYA GGEL I + GK+ E SKLIF+QI++A+ H+H +IHRDLKAEN+F+ N VK+GDFGFST+++ E L TFCGSPPYAAPELF+ + Y G VDIWALG+LLYFM+ G MPFR E+V LKK IL G Y P + LI+ +L+Q P +R I I N +W+ G +P +PF+ P + E T L+++ E L LGIT++HI Q + R+ +TG YRI+LHRV R + + ++P
Sbjct: 46 TPFEKLTQDMSQDEKVVREITLGKRIGFYRIRGEIGSGNFSQVKLGIHSLTKEKVAIKILDKTKLDQKTQRLLSREISSMEKLHHPNIIRLYEVVETLSKLHLVMEYAGGGELFGKISTEGKLSEPESKLIFSQIVSAVKHMHENQIIHRDLKAENVFYTSNTC----------------------------VKVGDFGFSTVSKKGEMLNTFCGSPPYAAPELFRDEHYIGIYVDIWALGVLLYFMVTGTMPFRAETVAKLKKSILEGTYSVPPHVSEPCHRLIRGVLQQIPTERYGIDCIMNDEWMQGVPYPTPLEPFQLDPKHLSETST-----------------LKEEENEVKSTLEHLGITEEHIRNNQGRDARSSITGVYRIILHRVQRKKALESVP 404
BLAST of Serine/threonine-protein kinase NIM1 vs. Ensembl Human
Match: NIM1K (NIM1 serine/threonine protein kinase [Source:HGNC Symbol;Acc:HGNC:28646]) HSP 1 Score: 395.201 bits (1014), Expect = 1.253e-132 Identity = 199/404 (49.26%), Postives = 260/404 (64.36%), Query Frame = 2
Query: 161 TSFDKYTEEMNCNENVKKEIMLGKRVGLYRIKGNLGNGNFSQVKLAVHLLTREEVAIKILDKTKLDAKTQKLLSREISSMERLHHPNIIRLYEVIEDIRKMHLVMEYASGGELHSLIVSHGKIPESISKLIFAQIIAAINHLHSQNVIHRDLKAENIFFAGNKSETNQDNSKLNSTTKKSDFTDNAFSRLVHVKLGDFGFSTLAQPAEKLTTFCGSPPYAAPELFQADWYYGTCVDIWALGILLYFMLCGCMPFRGESVGLLKKLILAGNYVCPNWLPVDAKNLIQQILKQNPDQRLTIQQIKNHQWLSGQHFPDAYQPFKATPL-MPEIETISHAXXXXXXXXXXXXPLEKDSIEASKMLLRLGITDKHIEVAQAQTVRNYVTGTYRIMLHRVHRNRRMSNIP 1369
T F+K T++M+ +E V +EI LGKR+G YRI+G +G+GNFSQVKL +H LT+E+VAIKILDKTKLD KTQ+LLSREISSME+LHHPNIIRLYEV+E + K+HLVMEYA GGEL I + GK+ E SKLIF+QI++A+ H+H +IHRDLKAEN+F+ N VK+GDFGFST+++ E L TFCGSPPYAAPELF+ + Y G VDIWALG+LLYFM+ G MPFR E+V LKK IL G Y P + LI+ +L+Q P +R I I N +W+ G +P +PF+ P + E T L+++ E L LGIT++HI Q + R+ +TG YRI+LHRV R + + ++P
Sbjct: 46 TPFEKLTQDMSQDEKVVREITLGKRIGFYRIRGEIGSGNFSQVKLGIHSLTKEKVAIKILDKTKLDQKTQRLLSREISSMEKLHHPNIIRLYEVVETLSKLHLVMEYAGGGELFGKISTEGKLSEPESKLIFSQIVSAVKHMHENQIIHRDLKAENVFYTSNTC----------------------------VKVGDFGFSTVSKKGEMLNTFCGSPPYAAPELFRDEHYIGIYVDIWALGVLLYFMVTGTMPFRAETVAKLKKSILEGTYSVPPHVSEPCHRLIRGVLQQIPTERYGIDCIMNDEWMQGVPYPTPLEPFQLDPKHLSETST-----------------LKEEENEVKSTLEHLGITEEHIRNNQGRDARSSITGVYRIILHRVQRKKALESVP 404
BLAST of Serine/threonine-protein kinase NIM1 vs. Ensembl Human
Match: MARK3 (microtubule affinity regulating kinase 3 [Source:HGNC Symbol;Acc:HGNC:6897]) HSP 1 Score: 258.07 bits (658), Expect = 1.178e-76 Identity = 122/305 (40.00%), Postives = 192/305 (62.95%), Query Frame = 2
Query: 236 VGLYRIKGNLGNGNFSQVKLAVHLLTREEVAIKILDKTKLDAKTQKLLSREISSMERLHHPNIIRLYEVIEDIRKMHLVMEYASGGELHSLIVSHGKIPESISKLIFAQIIAAINHLHSQNVIHRDLKAENIFFAGNKSETNQDNSKLNSTTKKSDFTDNAFSRLVHVKLGDFGFSTLAQPAEKLTTFCGSPPYAAPELFQADWYYGTCVDIWALGILLYFMLCGCMPFRGESVGLLKKLILAGNYVCPNWLPVDAKNLIQQILKQNPDQRLTIQQIKNHQWLSGQHFPDAYQPFKATPLMPEIE 1150
+G YR+ +G GNF++VKLA H+LT EVAIKI+DKT+L+ + + L RE+ M+ L+HPNI++L+EVIE + ++L+MEYASGGE+ +V+HG++ E ++ F QI++A+ + H + ++HRDLKAEN+ + +++K+ DFGFS KL TFCGSPPYAAPELFQ Y G VD+W+LG++LY ++ G +PF G+++ L++ +L G Y P ++ D +NL+++ L NP +R T++QI +W++ H D +PF + PE++
Sbjct: 53 IGNYRLLKTIGKGNFAKVKLARHILTGREVAIKIIDKTQLNPTSLQKLFREVRIMKILNHPNIVKLFEVIETEKTLYLIMEYASGGEVFDYLVAHGRMKEKEARSKFRQIVSAVQYCHQKRIVHRDLKAENLLLDAD----------------------------MNIKIADFGFSNEFTVGGKLDTFCGSPPYAAPELFQGKKYDGPEVDVWSLGVILYTLVSGSLPFDGQNLKELRERVLRGKYRIPFYMSTDCENLLKRFLVLNPIKRGTLEQIMKDRWINAGHEEDELKPF----VEPELD 325
BLAST of Serine/threonine-protein kinase NIM1 vs. Ensembl Human
Match: MARK3 (microtubule affinity regulating kinase 3 [Source:HGNC Symbol;Acc:HGNC:6897]) HSP 1 Score: 258.07 bits (658), Expect = 1.788e-76 Identity = 122/305 (40.00%), Postives = 192/305 (62.95%), Query Frame = 2
Query: 236 VGLYRIKGNLGNGNFSQVKLAVHLLTREEVAIKILDKTKLDAKTQKLLSREISSMERLHHPNIIRLYEVIEDIRKMHLVMEYASGGELHSLIVSHGKIPESISKLIFAQIIAAINHLHSQNVIHRDLKAENIFFAGNKSETNQDNSKLNSTTKKSDFTDNAFSRLVHVKLGDFGFSTLAQPAEKLTTFCGSPPYAAPELFQADWYYGTCVDIWALGILLYFMLCGCMPFRGESVGLLKKLILAGNYVCPNWLPVDAKNLIQQILKQNPDQRLTIQQIKNHQWLSGQHFPDAYQPFKATPLMPEIE 1150
+G YR+ +G GNF++VKLA H+LT EVAIKI+DKT+L+ + + L RE+ M+ L+HPNI++L+EVIE + ++L+MEYASGGE+ +V+HG++ E ++ F QI++A+ + H + ++HRDLKAEN+ + +++K+ DFGFS KL TFCGSPPYAAPELFQ Y G VD+W+LG++LY ++ G +PF G+++ L++ +L G Y P ++ D +NL+++ L NP +R T++QI +W++ H D +PF + PE++
Sbjct: 53 IGNYRLLKTIGKGNFAKVKLARHILTGREVAIKIIDKTQLNPTSLQKLFREVRIMKILNHPNIVKLFEVIETEKTLYLIMEYASGGEVFDYLVAHGRMKEKEARSKFRQIVSAVQYCHQKRIVHRDLKAENLLLDAD----------------------------MNIKIADFGFSNEFTVGGKLDTFCGSPPYAAPELFQGKKYDGPEVDVWSLGVILYTLVSGSLPFDGQNLKELRERVLRGKYRIPFYMSTDCENLLKRFLVLNPIKRGTLEQIMKDRWINAGHEEDELKPF----VEPELD 325
BLAST of Serine/threonine-protein kinase NIM1 vs. Ensembl Human
Match: MARK3 (microtubule affinity regulating kinase 3 [Source:HGNC Symbol;Acc:HGNC:6897]) HSP 1 Score: 258.07 bits (658), Expect = 2.185e-76 Identity = 122/305 (40.00%), Postives = 192/305 (62.95%), Query Frame = 2
Query: 236 VGLYRIKGNLGNGNFSQVKLAVHLLTREEVAIKILDKTKLDAKTQKLLSREISSMERLHHPNIIRLYEVIEDIRKMHLVMEYASGGELHSLIVSHGKIPESISKLIFAQIIAAINHLHSQNVIHRDLKAENIFFAGNKSETNQDNSKLNSTTKKSDFTDNAFSRLVHVKLGDFGFSTLAQPAEKLTTFCGSPPYAAPELFQADWYYGTCVDIWALGILLYFMLCGCMPFRGESVGLLKKLILAGNYVCPNWLPVDAKNLIQQILKQNPDQRLTIQQIKNHQWLSGQHFPDAYQPFKATPLMPEIE 1150
+G YR+ +G GNF++VKLA H+LT EVAIKI+DKT+L+ + + L RE+ M+ L+HPNI++L+EVIE + ++L+MEYASGGE+ +V+HG++ E ++ F QI++A+ + H + ++HRDLKAEN+ + +++K+ DFGFS KL TFCGSPPYAAPELFQ Y G VD+W+LG++LY ++ G +PF G+++ L++ +L G Y P ++ D +NL+++ L NP +R T++QI +W++ H D +PF + PE++
Sbjct: 53 IGNYRLLKTIGKGNFAKVKLARHILTGREVAIKIIDKTQLNPTSLQKLFREVRIMKILNHPNIVKLFEVIETEKTLYLIMEYASGGEVFDYLVAHGRMKEKEARSKFRQIVSAVQYCHQKRIVHRDLKAENLLLDAD----------------------------MNIKIADFGFSNEFTVGGKLDTFCGSPPYAAPELFQGKKYDGPEVDVWSLGVILYTLVSGSLPFDGQNLKELRERVLRGKYRIPFYMSTDCENLLKRFLVLNPIKRGTLEQIMKDRWINAGHEEDELKPF----VEPELD 325
BLAST of Serine/threonine-protein kinase NIM1 vs. Ensembl Celegans
Match: F49C5.4 (pep chromosome:WBcel235:II:12535760:12542569:1 gene:WBGene00009867.1 transcript:F49C5.4.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:F49C5.4) HSP 1 Score: 323.553 bits (828), Expect = 2.758e-104 Identity = 173/419 (41.29%), Postives = 250/419 (59.67%), Query Frame = 2
Query: 95 MNKKNSLDILPKISETEENNVETSFDKYTEEMNCNENVKKEIMLGKRVGLYRIKGNLGNGNFSQVKLAVHLLTREEVAIKILDKTKLDAKTQKLLSREISSMERLHHPNIIRLYEVIEDIRKMHLVMEYASGGELHSLIVSHGKIPESISKLIFAQIIAAINHLHSQNVIHRDLKAENIFFAGNKSETNQDNSKLNSTTKKSDFTDNAFSRLVHVKLGDFGFSTLAQPAEKLTTFCGSPPYAAPELFQADWYYGTCVDIWALGILLYFMLCGCMPFRGESVGLLKKLILAGNYVCPNWLPVDAKNLIQQILKQNPDQRLTIQQIKNHQWLSGQHFPDAYQPFKATPLMPEIETISHAXXXXXXXXXXXXPLEKDSIEAS--KMLLRLGITDKHI-EVAQAQTVRNYVTGTYRIMLHRVH 1342
N+ S LP + ++ + +T +++ ++N + V KE+ LG+R+G YR+ LG GNFS+VKL VH LT+E+VA+KI+DK K+DAK QKLLSREI +ME ++HPNI++L+EV+E + ++HLV+EYASGGEL++ + GK+ E +K +FAQI++A++H+HS+N++HRD+KAEN+ F+ + VKL DFGFS L + L TFCGSPPYAAPELFQ Y G VD+WALG+LL+FML G PF+ E+V +K LI AG Y P+++ + A LI+ +LK + QR I +K H W+ F +Y KAT + E EK +I+ K+L GI + I E + R+ V GTYRI+ +V
Sbjct: 87 FNRTVSAVSLPVGKQLKDMSEKTQYERAILQLNNDPVVHKEVALGRRIGFYRLGKELGAGNFSKVKLGVHQLTKEKVAVKIMDKAKMDAKAQKLLSREIQAMEEMNHPNIVKLFEVVETLTRVHLVIEYASGGELYTYVHERGKLTEGDAKPLFAQIVSAVSHMHSRNIVHRDIKAENVMFSSPNT----------------------------VKLVDFGFSCLVDREQMLRTFCGSPPYAAPELFQDTSYAGELVDVWALGVLLFFMLIGVTPFKAETVPDMKVLITAGKYQIPDYVSLLATELIKSMLKTDTGQRADIDSVKKHFWMRDCRFTKSYLSIKATAKIDNEE-------------------EKKAIDDKVWKILNNYGIVKEMIVEENLDKGPRDAVVGTYRIVQFQVQ 458
BLAST of Serine/threonine-protein kinase NIM1 vs. Ensembl Celegans
Match: par-1 (Serine/threonine-protein kinase par-1 [Source:UniProtKB/Swiss-Prot;Acc:Q9TW45]) HSP 1 Score: 254.218 bits (648), Expect = 4.600e-74 Identity = 121/299 (40.47%), Postives = 189/299 (63.21%), Query Frame = 2
Query: 236 VGLYRIKGNLGNGNFSQVKLAVHLLTREEVAIKILDKTKLDAKTQKLLSREISSMERLHHPNIIRLYEVIEDIRKMHLVMEYASGGELHSLIVSHGKIPESISKLIFAQIIAAINHLHSQNVIHRDLKAENIFFAGNKSETNQDNSKLNSTTKKSDFTDNAFSRLVHVKLGDFGFSTLAQPAEKLTTFCGSPPYAAPELFQADWYYGTCVDIWALGILLYFMLCGCMPFRGESVGLLKKLILAGNYVCPNWLPVDAKNLIQQILKQNPDQRLTIQQIKNHQWLSGQHFPDAYQPFKATP 1132
VG Y++ +G GNF++VKLA H++T EVAIKI+DKT L+ + + L RE+ M++L HPNI++LY+V+E + ++LV+EYASGGE+ +V+HG++ E ++ F QI++A+ +LHS+N+IHRDLKAEN+ +QD +++K+ DFGFS KL TFCGSPPYAAPELF Y G VD+W+LG++LY ++ G +PF G+++ L++ +L G Y P ++ D +NL+++ L NP +R ++ I +W++ + D +PF P
Sbjct: 103 VGKYKLLKTIGKGNFAKVKLAKHVITGHEVAIKIIDKTALNPSSLQKLFREVKIMKQLDHPNIVKLYQVMETEQTLYLVLEYASGGEVFDYLVAHGRMKEKEARAKFRQIVSAVQYLHSKNIIHRDLKAENLLL-------DQD---------------------MNIKIADFGFSNTFSLGNKLDTFCGSPPYAAPELFSGKKYDGPEVDVWSLGVILYTLVSGSLPFDGQNLKELRERVLRGKYRIPFYMSTDCENLLKKFLVINPQRRSSLDNIMKDRWMNVGYEDDELKPFIEPP 373
BLAST of Serine/threonine-protein kinase NIM1 vs. Ensembl Celegans
Match: par-1 (Serine/threonine-protein kinase par-1 [Source:UniProtKB/Swiss-Prot;Acc:Q9TW45]) HSP 1 Score: 254.218 bits (648), Expect = 4.600e-74 Identity = 121/299 (40.47%), Postives = 189/299 (63.21%), Query Frame = 2
Query: 236 VGLYRIKGNLGNGNFSQVKLAVHLLTREEVAIKILDKTKLDAKTQKLLSREISSMERLHHPNIIRLYEVIEDIRKMHLVMEYASGGELHSLIVSHGKIPESISKLIFAQIIAAINHLHSQNVIHRDLKAENIFFAGNKSETNQDNSKLNSTTKKSDFTDNAFSRLVHVKLGDFGFSTLAQPAEKLTTFCGSPPYAAPELFQADWYYGTCVDIWALGILLYFMLCGCMPFRGESVGLLKKLILAGNYVCPNWLPVDAKNLIQQILKQNPDQRLTIQQIKNHQWLSGQHFPDAYQPFKATP 1132
VG Y++ +G GNF++VKLA H++T EVAIKI+DKT L+ + + L RE+ M++L HPNI++LY+V+E + ++LV+EYASGGE+ +V+HG++ E ++ F QI++A+ +LHS+N+IHRDLKAEN+ +QD +++K+ DFGFS KL TFCGSPPYAAPELF Y G VD+W+LG++LY ++ G +PF G+++ L++ +L G Y P ++ D +NL+++ L NP +R ++ I +W++ + D +PF P
Sbjct: 103 VGKYKLLKTIGKGNFAKVKLAKHVITGHEVAIKIIDKTALNPSSLQKLFREVKIMKQLDHPNIVKLYQVMETEQTLYLVLEYASGGEVFDYLVAHGRMKEKEARAKFRQIVSAVQYLHSKNIIHRDLKAENLLL-------DQD---------------------MNIKIADFGFSNTFSLGNKLDTFCGSPPYAAPELFSGKKYDGPEVDVWSLGVILYTLVSGSLPFDGQNLKELRERVLRGKYRIPFYMSTDCENLLKKFLVINPQRRSSLDNIMKDRWMNVGYEDDELKPFIEPP 373
BLAST of Serine/threonine-protein kinase NIM1 vs. Ensembl Celegans
Match: par-1 (Serine/threonine-protein kinase par-1 [Source:UniProtKB/Swiss-Prot;Acc:Q9TW45]) HSP 1 Score: 254.218 bits (648), Expect = 4.600e-74 Identity = 121/299 (40.47%), Postives = 189/299 (63.21%), Query Frame = 2
Query: 236 VGLYRIKGNLGNGNFSQVKLAVHLLTREEVAIKILDKTKLDAKTQKLLSREISSMERLHHPNIIRLYEVIEDIRKMHLVMEYASGGELHSLIVSHGKIPESISKLIFAQIIAAINHLHSQNVIHRDLKAENIFFAGNKSETNQDNSKLNSTTKKSDFTDNAFSRLVHVKLGDFGFSTLAQPAEKLTTFCGSPPYAAPELFQADWYYGTCVDIWALGILLYFMLCGCMPFRGESVGLLKKLILAGNYVCPNWLPVDAKNLIQQILKQNPDQRLTIQQIKNHQWLSGQHFPDAYQPFKATP 1132
VG Y++ +G GNF++VKLA H++T EVAIKI+DKT L+ + + L RE+ M++L HPNI++LY+V+E + ++LV+EYASGGE+ +V+HG++ E ++ F QI++A+ +LHS+N+IHRDLKAEN+ +QD +++K+ DFGFS KL TFCGSPPYAAPELF Y G VD+W+LG++LY ++ G +PF G+++ L++ +L G Y P ++ D +NL+++ L NP +R ++ I +W++ + D +PF P
Sbjct: 103 VGKYKLLKTIGKGNFAKVKLAKHVITGHEVAIKIIDKTALNPSSLQKLFREVKIMKQLDHPNIVKLYQVMETEQTLYLVLEYASGGEVFDYLVAHGRMKEKEARAKFRQIVSAVQYLHSKNIIHRDLKAENLLL-------DQD---------------------MNIKIADFGFSNTFSLGNKLDTFCGSPPYAAPELFSGKKYDGPEVDVWSLGVILYTLVSGSLPFDGQNLKELRERVLRGKYRIPFYMSTDCENLLKKFLVINPQRRSSLDNIMKDRWMNVGYEDDELKPFIEPP 373
BLAST of Serine/threonine-protein kinase NIM1 vs. Ensembl Celegans
Match: par-1 (Serine/threonine-protein kinase par-1 [Source:UniProtKB/Swiss-Prot;Acc:Q9TW45]) HSP 1 Score: 254.218 bits (648), Expect = 4.600e-74 Identity = 121/299 (40.47%), Postives = 189/299 (63.21%), Query Frame = 2
Query: 236 VGLYRIKGNLGNGNFSQVKLAVHLLTREEVAIKILDKTKLDAKTQKLLSREISSMERLHHPNIIRLYEVIEDIRKMHLVMEYASGGELHSLIVSHGKIPESISKLIFAQIIAAINHLHSQNVIHRDLKAENIFFAGNKSETNQDNSKLNSTTKKSDFTDNAFSRLVHVKLGDFGFSTLAQPAEKLTTFCGSPPYAAPELFQADWYYGTCVDIWALGILLYFMLCGCMPFRGESVGLLKKLILAGNYVCPNWLPVDAKNLIQQILKQNPDQRLTIQQIKNHQWLSGQHFPDAYQPFKATP 1132
VG Y++ +G GNF++VKLA H++T EVAIKI+DKT L+ + + L RE+ M++L HPNI++LY+V+E + ++LV+EYASGGE+ +V+HG++ E ++ F QI++A+ +LHS+N+IHRDLKAEN+ +QD +++K+ DFGFS KL TFCGSPPYAAPELF Y G VD+W+LG++LY ++ G +PF G+++ L++ +L G Y P ++ D +NL+++ L NP +R ++ I +W++ + D +PF P
Sbjct: 103 VGKYKLLKTIGKGNFAKVKLAKHVITGHEVAIKIIDKTALNPSSLQKLFREVKIMKQLDHPNIVKLYQVMETEQTLYLVLEYASGGEVFDYLVAHGRMKEKEARAKFRQIVSAVQYLHSKNIIHRDLKAENLLL-------DQD---------------------MNIKIADFGFSNTFSLGNKLDTFCGSPPYAAPELFSGKKYDGPEVDVWSLGVILYTLVSGSLPFDGQNLKELRERVLRGKYRIPFYMSTDCENLLKKFLVINPQRRSSLDNIMKDRWMNVGYEDDELKPFIEPP 373
BLAST of Serine/threonine-protein kinase NIM1 vs. Ensembl Fly
Match: CG4629 (gene:FBgn0031299 transcript:FBtr0332570) HSP 1 Score: 266.159 bits (679), Expect = 2.237e-81 Identity = 135/311 (43.41%), Postives = 192/311 (61.74%), Query Frame = 2
Query: 167 FDKYTEEMNCNENVKKEIMLGKRVGLYRIKGNLGNGNFSQVKLAVHLLTREEVAIKI--LDKTKLDAKTQKLLSREISSMERLHHPNIIRLYEVIEDIRKMHLVMEYASGGELHSLIVSHGKIPESISKLIFAQIIAAINHLHSQNVIHRDLKAENIFFAGNKSETNQDNSKLNSTTKKSDFTDNAFSRLVHVKLGDFGFST--LAQPAEKLTTFCGSPPYAAPELFQADWYYGTCVDIWALGILLYFMLCGCMPFRGESVGLLKKLILAGNYVCPNWLPVDAKNLIQQILKQNPDQRLTIQQIKNHQWLS 1087
+ + T+ + C+ E+ +G+R+GLYR G++G GNFS+VKLAVH LTR++VAIK+ LD+ LDAK ++LS EI+++E +HHPNI+RL+EV+E + +++LV E+ GGEL++ I G + E + + Q++ A+ H+HS +HRD+KAEN+ +KL DFGFST + +KL TFCGSPPYAAPELF D Y G VD+WALGILLYFM+ G MPFR ++ LK IL G+Y+ P L + LIQ+IL P QR TI + N Q+++
Sbjct: 40 YQRLTKALQCDPRCGHEVTIGRRIGLYRFCGDIGRGNFSKVKLAVHQLTRDKVAIKVVDLDRAGLDAKALRMLSSEIATLECVHHPNILRLFEVVETLGRVYLVTEWIRGGELYNHITQGGPLREIHAAPLLKQLLLAVKHMHSLGYVHRDIKAENVLLLSED----------------------------RLKLADFGFSTQLINGANQKLDTFCGSPPYAAPELFSDDHYIGAPVDVWALGILLYFMVVGNMPFRAPTIPGLKAAILKGDYLLPGQLSLPCIRLIQRILIHIPAQRPTIDDMLNSQFVT 322
BLAST of Serine/threonine-protein kinase NIM1 vs. Ensembl Fly
Match: CG4629 (gene:FBgn0031299 transcript:FBtr0332571) HSP 1 Score: 266.159 bits (679), Expect = 2.237e-81 Identity = 135/311 (43.41%), Postives = 192/311 (61.74%), Query Frame = 2
Query: 167 FDKYTEEMNCNENVKKEIMLGKRVGLYRIKGNLGNGNFSQVKLAVHLLTREEVAIKI--LDKTKLDAKTQKLLSREISSMERLHHPNIIRLYEVIEDIRKMHLVMEYASGGELHSLIVSHGKIPESISKLIFAQIIAAINHLHSQNVIHRDLKAENIFFAGNKSETNQDNSKLNSTTKKSDFTDNAFSRLVHVKLGDFGFST--LAQPAEKLTTFCGSPPYAAPELFQADWYYGTCVDIWALGILLYFMLCGCMPFRGESVGLLKKLILAGNYVCPNWLPVDAKNLIQQILKQNPDQRLTIQQIKNHQWLS 1087
+ + T+ + C+ E+ +G+R+GLYR G++G GNFS+VKLAVH LTR++VAIK+ LD+ LDAK ++LS EI+++E +HHPNI+RL+EV+E + +++LV E+ GGEL++ I G + E + + Q++ A+ H+HS +HRD+KAEN+ +KL DFGFST + +KL TFCGSPPYAAPELF D Y G VD+WALGILLYFM+ G MPFR ++ LK IL G+Y+ P L + LIQ+IL P QR TI + N Q+++
Sbjct: 40 YQRLTKALQCDPRCGHEVTIGRRIGLYRFCGDIGRGNFSKVKLAVHQLTRDKVAIKVVDLDRAGLDAKALRMLSSEIATLECVHHPNILRLFEVVETLGRVYLVTEWIRGGELYNHITQGGPLREIHAAPLLKQLLLAVKHMHSLGYVHRDIKAENVLLLSED----------------------------RLKLADFGFSTQLINGANQKLDTFCGSPPYAAPELFSDDHYIGAPVDVWALGILLYFMVVGNMPFRAPTIPGLKAAILKGDYLLPGQLSLPCIRLIQRILIHIPAQRPTIDDMLNSQFVT 322
BLAST of Serine/threonine-protein kinase NIM1 vs. Ensembl Fly
Match: KP78b (gene:FBgn0026063 transcript:FBtr0082408) HSP 1 Score: 252.292 bits (643), Expect = 8.161e-76 Identity = 120/304 (39.47%), Postives = 191/304 (62.83%), Query Frame = 2
Query: 227 GKRVGLYRIKGNLGNGNFSQVKLAVHLLTREEVAIKILDKTKLDAKTQKLLSREISSMERLHHPNIIRLYEVIEDIRKMHLVMEYASGGELHSLIVSHGKIPESISKLIFAQIIAAINHLHSQNVIHRDLKAENIFFAGNKSETNQDNSKLNSTTKKSDFTDNAFSRLVHVKLGDFGFSTLAQPAEKLTTFCGSPPYAAPELFQADWYYGTCVDIWALGILLYFMLCGCMPFRGESVGLLKKLILAGNYVCPNWLPVDAKNLIQQILKQNPDQRLTIQQIKNHQWLS-GQHFPDAYQPFKATPL 1135
G G+Y+I LG GNF++VKLA+HL T EVAIK++DKT L+ ++ L RE++ M++L+HPNI+RL +VIE R ++LVMEY SGGEL + +V +G++ E ++++F Q+++AI + HS++++HRDLKAEN+ + + +K+ DFGFST +P L TFCGSPPYAAPELF+ Y G VD W+LG++LY ++ G +PF G ++ L+ +L G Y P ++ ++ ++LI++ L NP QR ++ + +W++ G + +PF+ P+
Sbjct: 57 GNGYGVYKIIKTLGKGNFAKVKLAIHLPTGREVAIKLIDKTALNTIARQKLYREVNIMKKLNHPNIVRLLQVIESERTLYLVMEYVSGGELFNYLVKNGRMRERDARVLFRQLVSAIEYCHSKSIVHRDLKAENLL----------------------------LDQQMKLKIADFGFSTTFEPKAPLETFCGSPPYAAPELFRGKKYSGPEVDSWSLGVVLYTLVSGSLPFDGTNLKELRDRVLRGKYRVPYYVSIECESLIRKFLVLNPTQRTSLSAVMADRWINMGYEQGNGLRPFQEKPM 332
BLAST of Serine/threonine-protein kinase NIM1 vs. Ensembl Fly
Match: KP78b (gene:FBgn0026063 transcript:FBtr0301427) HSP 1 Score: 252.292 bits (643), Expect = 8.161e-76 Identity = 120/304 (39.47%), Postives = 191/304 (62.83%), Query Frame = 2
Query: 227 GKRVGLYRIKGNLGNGNFSQVKLAVHLLTREEVAIKILDKTKLDAKTQKLLSREISSMERLHHPNIIRLYEVIEDIRKMHLVMEYASGGELHSLIVSHGKIPESISKLIFAQIIAAINHLHSQNVIHRDLKAENIFFAGNKSETNQDNSKLNSTTKKSDFTDNAFSRLVHVKLGDFGFSTLAQPAEKLTTFCGSPPYAAPELFQADWYYGTCVDIWALGILLYFMLCGCMPFRGESVGLLKKLILAGNYVCPNWLPVDAKNLIQQILKQNPDQRLTIQQIKNHQWLS-GQHFPDAYQPFKATPL 1135
G G+Y+I LG GNF++VKLA+HL T EVAIK++DKT L+ ++ L RE++ M++L+HPNI+RL +VIE R ++LVMEY SGGEL + +V +G++ E ++++F Q+++AI + HS++++HRDLKAEN+ + + +K+ DFGFST +P L TFCGSPPYAAPELF+ Y G VD W+LG++LY ++ G +PF G ++ L+ +L G Y P ++ ++ ++LI++ L NP QR ++ + +W++ G + +PF+ P+
Sbjct: 57 GNGYGVYKIIKTLGKGNFAKVKLAIHLPTGREVAIKLIDKTALNTIARQKLYREVNIMKKLNHPNIVRLLQVIESERTLYLVMEYVSGGELFNYLVKNGRMRERDARVLFRQLVSAIEYCHSKSIVHRDLKAENLL----------------------------LDQQMKLKIADFGFSTTFEPKAPLETFCGSPPYAAPELFRGKKYSGPEVDSWSLGVVLYTLVSGSLPFDGTNLKELRDRVLRGKYRVPYYVSIECESLIRKFLVLNPTQRTSLSAVMADRWINMGYEQGNGLRPFQEKPM 332
BLAST of Serine/threonine-protein kinase NIM1 vs. Ensembl Fly
Match: KP78a (gene:FBgn0026064 transcript:FBtr0301426) HSP 1 Score: 250.366 bits (638), Expect = 3.310e-74 Identity = 125/329 (37.99%), Postives = 197/329 (59.88%), Query Frame = 2
Query: 152 NVETSFDKYTEEMNCNENVKKEIMLGKRVGLYRIKGNLGNGNFSQVKLAVHLLTREEVAIKILDKTKLDAKTQKLLSREISSMERLHHPNIIRLYEVIEDIRKMHLVMEYASGGELHSLIVSHGKIPESISKLIFAQIIAAINHLHSQNVIHRDLKAENIFFAGNKSETNQDNSKLNSTTKKSDFTDNAFSRLVHVKLGDFGFSTLAQPAEKLTTFCGSPPYAAPELFQADWYYGTCVDIWALGILLYFMLCGCMPFRGESVGLLKKLILAGNYVCPNWLPVDAKNLIQQILKQNPDQRLTIQQIKNHQWLS-GQHFPDAYQPFKATPL 1135
N ++ F Y +N N N G+Y+I LG GNF++VKLA+H+ T EVAIK++DKT+L+ ++ L RE+ M+ L+HPNI+RL++VIE R ++LVMEYAS GEL +V +G++ E +++IF Q+++AI + HS+ V+HRDLKAEN+ + +++K+ DFGF P +L TFCGSPPYAAPELF Y G VD W+LG++LY ++ G +PF G ++ L++ +L G Y P ++ +D +NL+++ L NP +R ++ + + +W++ G D +PF+ P+
Sbjct: 81 NTDSKFQSY---VNGNGN-----------GVYKIIKTLGKGNFAKVKLAIHVPTGREVAIKVIDKTQLNTSARQKLYREVKIMKLLNHPNIVRLFQVIESERTLYLVMEYASRGELFDHLVKNGRMRERDARVIFRQLVSAIQYCHSKFVVHRDLKAENLL----------------------------LDQHMNIKIADFGFGNTFDPNAQLETFCGSPPYAAPELFMGRKYAGPEVDAWSLGVVLYTLVSGSLPFDGGTLKELRERVLRGKYRVPYYISMDCENLMRKFLVLNPAKRTSLSAVMSDKWINLGHDESDRLRPFREKPM 367
BLAST of Serine/threonine-protein kinase NIM1 vs. Ensembl Zebrafish
Match: nim1k (NIM1 serine/threonine protein kinase [Source:ZFIN;Acc:ZDB-GENE-130530-744]) HSP 1 Score: 385.956 bits (990), Expect = 3.523e-129 Identity = 189/424 (44.58%), Postives = 267/424 (62.97%), Query Frame = 2
Query: 98 NKKNSLDILPKISETEENNVETSFDKYTEEMNCNENVKKEIMLGKRVGLYRIKGNLGNGNFSQVKLAVHLLTREEVAIKILDKTKLDAKTQKLLSREISSMERLHHPNIIRLYEVIEDIRKMHLVMEYASGGELHSLIVSHGKIPESISKLIFAQIIAAINHLHSQNVIHRDLKAENIFFAGNKSETNQDNSKLNSTTKKSDFTDNAFSRLVHVKLGDFGFSTLAQPAEKLTTFCGSPPYAAPELFQADWYYGTCVDIWALGILLYFMLCGCMPFRGESVGLLKKLILAGNYVCPNWLPVDAKNLIQQILKQNPDQRLTIQQIKNHQWLSGQHFPDAYQPFKATPLMPEIETISHAXXXXXXXXXXXXPLEKDSIEASKMLLRLGITDKHIEVAQAQTVRNYVTGTYRIMLHRVHRNRRMSNIP 1369
++++S DI T+ + T +K +M +E + +E+ +G+R+G Y+I+G +G GNFS VKL +H LT+++VAIKILDKTKLD KTQ+LLSREISSME+LHHPNIIRLYEV+E + ++HLVMEYA GGEL++ I + GK+ + SK++F+Q+++A+ H+H N+IHRDLKAEN+F+ + VK+GDFGFST+++ E L TFCGSPPYAAPELF+ + Y G CVDIWALG++L+FM+ G MPFR E+V LK+ IL G Y P W+P + LI+ IL+ P R +++Q+ +WL FP A +PFK P S LE+ IE L GIT +HI Q + R+ +TG YRI+LHR H+ + + ++P
Sbjct: 26 SRQDSSDICTDDEGTQARRL-TPLEKLNLDMCQDEKMVRELTVGRRIGFYKIRGEIGCGNFSHVKLGIHALTKDKVAIKILDKTKLDQKTQRLLSREISSMEKLHHPNIIRLYEVVETLTRLHLVMEYAGGGELYTKISTEGKLSDIDSKIVFSQVLSAVKHMHDNNIIHRDLKAENVFYTCSTC----------------------------VKVGDFGFSTVSRRDETLNTFCGSPPYAAPELFRDEHYIGVCVDIWALGVMLFFMVTGTMPFRAETVAKLKRCILDGTYTVPTWVPEPCQKLIRGILQPQPSDRCSVEQMMACEWLQPVEFPKAMEPFKLDP--------------HHLVESGPGELEEHEIEVRNALEMFGITAEHIRNNQGKDCRSSITGVYRILLHRAHKRQAVESMP 406
BLAST of Serine/threonine-protein kinase NIM1 vs. Ensembl Zebrafish
Match: nim1k (NIM1 serine/threonine protein kinase [Source:ZFIN;Acc:ZDB-GENE-130530-744]) HSP 1 Score: 385.956 bits (990), Expect = 3.523e-129 Identity = 189/424 (44.58%), Postives = 267/424 (62.97%), Query Frame = 2
Query: 98 NKKNSLDILPKISETEENNVETSFDKYTEEMNCNENVKKEIMLGKRVGLYRIKGNLGNGNFSQVKLAVHLLTREEVAIKILDKTKLDAKTQKLLSREISSMERLHHPNIIRLYEVIEDIRKMHLVMEYASGGELHSLIVSHGKIPESISKLIFAQIIAAINHLHSQNVIHRDLKAENIFFAGNKSETNQDNSKLNSTTKKSDFTDNAFSRLVHVKLGDFGFSTLAQPAEKLTTFCGSPPYAAPELFQADWYYGTCVDIWALGILLYFMLCGCMPFRGESVGLLKKLILAGNYVCPNWLPVDAKNLIQQILKQNPDQRLTIQQIKNHQWLSGQHFPDAYQPFKATPLMPEIETISHAXXXXXXXXXXXXPLEKDSIEASKMLLRLGITDKHIEVAQAQTVRNYVTGTYRIMLHRVHRNRRMSNIP 1369
++++S DI T+ + T +K +M +E + +E+ +G+R+G Y+I+G +G GNFS VKL +H LT+++VAIKILDKTKLD KTQ+LLSREISSME+LHHPNIIRLYEV+E + ++HLVMEYA GGEL++ I + GK+ + SK++F+Q+++A+ H+H N+IHRDLKAEN+F+ + VK+GDFGFST+++ E L TFCGSPPYAAPELF+ + Y G CVDIWALG++L+FM+ G MPFR E+V LK+ IL G Y P W+P + LI+ IL+ P R +++Q+ +WL FP A +PFK P S LE+ IE L GIT +HI Q + R+ +TG YRI+LHR H+ + + ++P
Sbjct: 26 SRQDSSDICTDDEGTQARRL-TPLEKLNLDMCQDEKMVRELTVGRRIGFYKIRGEIGCGNFSHVKLGIHALTKDKVAIKILDKTKLDQKTQRLLSREISSMEKLHHPNIIRLYEVVETLTRLHLVMEYAGGGELYTKISTEGKLSDIDSKIVFSQVLSAVKHMHDNNIIHRDLKAENVFYTCSTC----------------------------VKVGDFGFSTVSRRDETLNTFCGSPPYAAPELFRDEHYIGVCVDIWALGVMLFFMVTGTMPFRAETVAKLKRCILDGTYTVPTWVPEPCQKLIRGILQPQPSDRCSVEQMMACEWLQPVEFPKAMEPFKLDP--------------HHLVESGPGELEEHEIEVRNALEMFGITAEHIRNNQGKDCRSSITGVYRILLHRAHKRQAVESMP 406
BLAST of Serine/threonine-protein kinase NIM1 vs. Ensembl Zebrafish
Match: nim1k (NIM1 serine/threonine protein kinase [Source:ZFIN;Acc:ZDB-GENE-130530-744]) HSP 1 Score: 385.956 bits (990), Expect = 3.523e-129 Identity = 189/424 (44.58%), Postives = 267/424 (62.97%), Query Frame = 2
Query: 98 NKKNSLDILPKISETEENNVETSFDKYTEEMNCNENVKKEIMLGKRVGLYRIKGNLGNGNFSQVKLAVHLLTREEVAIKILDKTKLDAKTQKLLSREISSMERLHHPNIIRLYEVIEDIRKMHLVMEYASGGELHSLIVSHGKIPESISKLIFAQIIAAINHLHSQNVIHRDLKAENIFFAGNKSETNQDNSKLNSTTKKSDFTDNAFSRLVHVKLGDFGFSTLAQPAEKLTTFCGSPPYAAPELFQADWYYGTCVDIWALGILLYFMLCGCMPFRGESVGLLKKLILAGNYVCPNWLPVDAKNLIQQILKQNPDQRLTIQQIKNHQWLSGQHFPDAYQPFKATPLMPEIETISHAXXXXXXXXXXXXPLEKDSIEASKMLLRLGITDKHIEVAQAQTVRNYVTGTYRIMLHRVHRNRRMSNIP 1369
++++S DI T+ + T +K +M +E + +E+ +G+R+G Y+I+G +G GNFS VKL +H LT+++VAIKILDKTKLD KTQ+LLSREISSME+LHHPNIIRLYEV+E + ++HLVMEYA GGEL++ I + GK+ + SK++F+Q+++A+ H+H N+IHRDLKAEN+F+ + VK+GDFGFST+++ E L TFCGSPPYAAPELF+ + Y G CVDIWALG++L+FM+ G MPFR E+V LK+ IL G Y P W+P + LI+ IL+ P R +++Q+ +WL FP A +PFK P S LE+ IE L GIT +HI Q + R+ +TG YRI+LHR H+ + + ++P
Sbjct: 26 SRQDSSDICTDDEGTQARRL-TPLEKLNLDMCQDEKMVRELTVGRRIGFYKIRGEIGCGNFSHVKLGIHALTKDKVAIKILDKTKLDQKTQRLLSREISSMEKLHHPNIIRLYEVVETLTRLHLVMEYAGGGELYTKISTEGKLSDIDSKIVFSQVLSAVKHMHDNNIIHRDLKAENVFYTCSTC----------------------------VKVGDFGFSTVSRRDETLNTFCGSPPYAAPELFRDEHYIGVCVDIWALGVMLFFMVTGTMPFRAETVAKLKRCILDGTYTVPTWVPEPCQKLIRGILQPQPSDRCSVEQMMACEWLQPVEFPKAMEPFKLDP--------------HHLVESGPGELEEHEIEVRNALEMFGITAEHIRNNQGKDCRSSITGVYRILLHRAHKRQAVESMP 406
BLAST of Serine/threonine-protein kinase NIM1 vs. Ensembl Zebrafish
Match: si:ch211-22d5.2 (si:ch211-22d5.2 [Source:ZFIN;Acc:ZDB-GENE-091204-283]) HSP 1 Score: 333.183 bits (853), Expect = 1.005e-108 Identity = 165/404 (40.84%), Postives = 252/404 (62.38%), Query Frame = 2
Query: 161 TSFDKYTEEMNCNENVKKEIMLGKRVGLYRIKGNLGNGNFSQVKLAVHLLTREEVAIKILDKTKLDAKTQKLLSREISSMERLHHPNIIRLYEVIEDIRKMHLVMEYASGGELHSLIVSHGKIPESISKLIFAQIIAAINHLHSQNVIHRDLKAENIFFAGNKSETNQDNSKLNSTTKKSDFTDNAFSRLVHVKLGDFGFST-LAQPAEKLTTFCGSPPYAAPELFQADWYYGTCVDIWALGILLYFMLCGCMPFRGESVGLLKKLILAGNYVCPNWLPVDAKNLIQQILKQNPDQRLTIQQIKNHQWLSGQHFPDAYQPFKATPLMPEIETISHAXXXXXXXXXXXXPLEKDSIEASKMLLRLGITDKHIEVAQAQTVRNYVTGTYRIMLHRVHRNRRMSNIP 1369
T +K T EM +EN KE+ +GKRVG Y+++G +G GNFS+VKLA H LT+++VA+KI+DK +LD +TQ++L+REIS+ME L+HPN+++L+EV+E +++L++E+A GG+LH+ I S GK+ + SK++FAQI++A+ ++H N+IHRDLKAEN+ + N +K+ DFGFST + + L TFCGSPPYAAPELF+ D Y G VD+WA+G+LL+FM+ G +PFR ++V L++ +L G YV P W+ + LI+ ILK P +R + Q+ +WL F+ P + ++ I + L++D E +L +LG+T +HI Q + +R+ + G YRI+LHRV RNR ++P
Sbjct: 41 TPLEKLTLEMCKDENTVKELTVGKRVGFYKVRGQIGCGNFSKVKLATHALTKDKVAVKIMDKMRLDVQTQRMLTREISNMESLYHPNLLQLFEVLETPSRLYLILEFAGGGDLHTRISSGGKLTDLESKIVFAQILSAVKYMHENNIIHRDLKAENVLYTTNGC----------------------------IKVADFGFSTKVNNRNQALDTFCGSPPYAAPELFKDDSYIGPPVDVWAMGVLLFFMVTGSLPFRADTVPKLRQSVLEGAYVLPIWVSAPCQRLIRGILKPEPSERCALDQMLGCEWLLPVEL------FRPIPPLYQLNPIH-------LLEAGPGELDRDQEEVKGILEKLGVTPEHILNNQGKRIRSPINGVYRILLHRVKRNRGAESVP 403
BLAST of Serine/threonine-protein kinase NIM1 vs. Ensembl Zebrafish
Match: si:ch211-22d5.2 (si:ch211-22d5.2 [Source:ZFIN;Acc:ZDB-GENE-091204-283]) HSP 1 Score: 332.798 bits (852), Expect = 1.063e-108 Identity = 165/404 (40.84%), Postives = 252/404 (62.38%), Query Frame = 2
Query: 161 TSFDKYTEEMNCNENVKKEIMLGKRVGLYRIKGNLGNGNFSQVKLAVHLLTREEVAIKILDKTKLDAKTQKLLSREISSMERLHHPNIIRLYEVIEDIRKMHLVMEYASGGELHSLIVSHGKIPESISKLIFAQIIAAINHLHSQNVIHRDLKAENIFFAGNKSETNQDNSKLNSTTKKSDFTDNAFSRLVHVKLGDFGFST-LAQPAEKLTTFCGSPPYAAPELFQADWYYGTCVDIWALGILLYFMLCGCMPFRGESVGLLKKLILAGNYVCPNWLPVDAKNLIQQILKQNPDQRLTIQQIKNHQWLSGQHFPDAYQPFKATPLMPEIETISHAXXXXXXXXXXXXPLEKDSIEASKMLLRLGITDKHIEVAQAQTVRNYVTGTYRIMLHRVHRNRRMSNIP 1369
T +K T EM +EN KE+ +GKRVG Y+++G +G GNFS+VKLA H LT+++VA+KI+DK +LD +TQ++L+REIS+ME L+HPN+++L+EV+E +++L++E+A GG+LH+ I S GK+ + SK++FAQI++A+ ++H N+IHRDLKAEN+ + N +K+ DFGFST + + L TFCGSPPYAAPELF+ D Y G VD+WA+G+LL+FM+ G +PFR ++V L++ +L G YV P W+ + LI+ ILK P +R + Q+ +WL F+ P + ++ I + L++D E +L +LG+T +HI Q + +R+ + G YRI+LHRV RNR ++P
Sbjct: 41 TPLEKLTLEMCKDENTVKELTVGKRVGFYKVRGQIGCGNFSKVKLATHALTKDKVAVKIMDKMRLDVQTQRMLTREISNMESLYHPNLLQLFEVLETPSRLYLILEFAGGGDLHTRISSGGKLTDLESKIVFAQILSAVKYMHENNIIHRDLKAENVLYTTNGC----------------------------IKVADFGFSTKVNNRNQALDTFCGSPPYAAPELFKDDSYIGPPVDVWAMGVLLFFMVTGSLPFRADTVPKLRQSVLEGAYVLPIWVSAPCQRLIRGILKPEPSERCALDQMLGCEWLLPVEL------FRPIPPLYQLNPIH-------LLEAGPGELDRDQEEVKGILEKLGVTPEHILNNQGKRIRSPINGVYRILLHRVKRNRGAESVP 403
BLAST of Serine/threonine-protein kinase NIM1 vs. Ensembl Xenopus
Match: NIM1K (NIM1 serine/threonine protein kinase [Source:NCBI gene;Acc:100491267]) HSP 1 Score: 397.897 bits (1021), Expect = 7.568e-134 Identity = 205/443 (46.28%), Postives = 285/443 (64.33%), Query Frame = 2
Query: 98 NKKNSLDILPKISETEENNVE------TSFDKYTEEMNCNENVKKEIMLGKRVGLYRIKGNLGNGNFSQVKLAVHLLTREEVAIKILDKTKLDAKTQKLLSREISSMERLHHPNIIRLYEVIEDIRKMHLVMEYASGGELHSLIVSHGKIPESISKLIFAQIIAAINHLHSQNVIHRDLKAENIFFAGNKSETNQDNSKLNSTTKKSDFTDNAFSRLVHVKLGDFGFSTLAQPAEKLTTFCGSPPYAAPELFQADWYYGTCVDIWALGILLYFMLCGCMPFRGESVGLLKKLILAGNYVCPNWLPVDAKNLIQQILKQNPDQRLTIQQIKNHQWLSGQHFPDAYQPFKATPLMPEIETISHAXXXXXXXXXXXXPLEKDSIEASKMLLRLGITDKHIEVAQAQTVRNYVTGTYRIMLHRVHRNRRMSNIPHSLSNPELGRKFF 1408
N+++SL+ + SE E + E T F+K T++M+ +E KE+ LGKR+GLYR++G +G+GNFSQVKL +H+LT+E+VAIKILDKTKLD KTQ+LLSREISSME+LHHPN+IRLYEV+E + K+HLVMEYA+GGEL + I + GK+ ES SK+IF+QI++A+ H+H +IHRDLKAEN+F+ N VK+GDFGFST+++ E L TFCGSPPYAAPELF+ + Y G VDIWALGILLYFM+ G MPFR ++V LKK IL G Y P ++ + LI+ +L+ P R +I I N +W++G +P +P K P + + PL+ + E +L LGIT++HI + + R+ +TG YRI+LHRV + R + ++P ++NPE K+
Sbjct: 20 NRQDSLEDYVR-SECYEQDAEGKQRKLTPFEKLTQDMSRDEKTVKELTLGKRIGLYRMRGEIGSGNFSQVKLGIHVLTKEKVAIKILDKTKLDQKTQRLLSREISSMEKLHHPNVIRLYEVVETLSKLHLVMEYAAGGELFAKINTEGKLSESESKVIFSQILSAVKHMHDNQIIHRDLKAENVFYTSNTC----------------------------VKVGDFGFSTISKKDETLNTFCGSPPYAAPELFRDEHYVGVFVDIWALGILLYFMVSGSMPFRADTVAKLKKCILDGTYTIPPYVSEPCQKLIKGMLQPIPSDRCSIDYIINSEWMNGIQYPKPLEPLKLDP----------------KHLFDGTPLKDEDSEVKNILDNLGITEEHIINNRGKDSRSSITGIYRIILHRVQKKRAVESVP-IITNPEPKEKYM 416
BLAST of Serine/threonine-protein kinase NIM1 vs. Ensembl Xenopus
Match: ENSXETT00000045494.1 (MAP/microtubule affinity-regulating kinase 4 [Source:NCBI gene;Acc:100491038]) HSP 1 Score: 263.848 bits (673), Expect = 3.865e-80 Identity = 153/456 (33.55%), Postives = 243/456 (53.29%), Query Frame = 2
Query: 236 VGLYRIKGNLGNGNFSQVKLAVHLLTREEVAIKILDKTKLDAKTQKLLSREISSMERLHHPNIIRLYEVIEDIRKMHLVMEYASGGELHSLIVSHGKIPESISKLIFAQIIAAINHLHSQNVIHRDLKAENIFFAGNKSETNQDNSKLNSTTKKSDFTDNAFSRLVHVKLGDFGFSTLAQPAEKLTTFCGSPPYAAPELFQADWYYGTCVDIWALGILLYFMLCGCMPFRGESVGLLKKLILAGNYVCPNWLPVDAKNLIQQILKQNPDQRLTIQQIKNHQWLSGQHFPDAYQPFKATPLMPEIETISHAXXXXXXXXXXXXPLEKDSIEASK--MLLRLGITDKHIEVAQAQTVRNYVTGTYRIMLHRVHRNRRMSNIPHSLSNPELGRKFFNSRKFQSKVITRKVNGAGPKPKTSSCPEKGIEPNQKSSKMC------NXXXXXXXXSCFIFLL 1579
VG YR+ +G GNF++VKLA H+LT EVAIKI+DKT+L+ + + L RE+ M+ L+HPNI++L+EVIE + ++L+MEYASGGE+ +VSHG++ E ++ F QI++A+++ H +N++HRDLKAEN+ SE+N +K+ DFGFS P KL TFCGSPPYAAPELFQ Y G VD+W+LG++LY ++ G +PF G+++ L++ +L G Y P ++ D + ++++ L NP +R T+ QI N +W++ D +P+K E+D+ + + ++L +G + + I+ A + N V TY ++ + S +LS L R S+V + NGA S ++G + ++ S C + + CF+FL
Sbjct: 48 VGNYRLLRTIGKGNFAKVKLARHVLTGREVAIKIIDKTQLNPSSLQKLFREVRIMKGLNHPNIVKLFEVIETEKTLYLIMEYASGGEVFDYLVSHGRMKEKEARAKFRQIVSAVHYCHQKNIVHRDLKAENLLL---DSESN-------------------------IKIADFGFSNEFTPGGKLDTFCGSPPYAAPELFQGKRYNGPEVDVWSLGVILYTLVSGSLPFDGQNLKELRERVLRGKYRIPFYMSTDCEGVLRRFLVLNPSKRCTLDQIMNDKWMNIGFDSDDLKPYKEP--------------------------EEDNADPKRIEIMLEMGYSREEIKDALSSNKYNEVMATYLLLGRKPESEGGESRSDSTLS---LSR---------SRVASDVTNGATKSSSVHSKNQRGYQRQRRHSDFCMCQDLYKLRHYSEAYVCFLFLF 437
BLAST of Serine/threonine-protein kinase NIM1 vs. Ensembl Xenopus
Match: ENSXETT00000045307.1 (MAP/microtubule affinity-regulating kinase 4 [Source:NCBI gene;Acc:100491038]) HSP 1 Score: 262.692 bits (670), Expect = 7.379e-79 Identity = 125/296 (42.23%), Postives = 190/296 (64.19%), Query Frame = 2
Query: 236 VGLYRIKGNLGNGNFSQVKLAVHLLTREEVAIKILDKTKLDAKTQKLLSREISSMERLHHPNIIRLYEVIEDIRKMHLVMEYASGGELHSLIVSHGKIPESISKLIFAQIIAAINHLHSQNVIHRDLKAENIFFAGNKSETNQDNSKLNSTTKKSDFTDNAFSRLVHVKLGDFGFSTLAQPAEKLTTFCGSPPYAAPELFQADWYYGTCVDIWALGILLYFMLCGCMPFRGESVGLLKKLILAGNYVCPNWLPVDAKNLIQQILKQNPDQRLTIQQIKNHQWLSGQHFPDAYQPFK 1123
VG YR+ +G GNF++VKLA H+LT EVAIKI+DKT+L+ + + L RE+ M+ L+HPNI++L+EVIE + ++L+MEYASGGE+ +VSHG++ E ++ F QI++A+++ H +N++HRDLKAEN+ SE+N +K+ DFGFS P KL TFCGSPPYAAPELFQ Y G VD+W+LG++LY ++ G +PF G+++ L++ +L G Y P ++ D + ++++ L NP +R T+ QI N +W++ D +P+K
Sbjct: 48 VGNYRLLRTIGKGNFAKVKLARHVLTGREVAIKIIDKTQLNPSSLQKLFREVRIMKGLNHPNIVKLFEVIETEKTLYLIMEYASGGEVFDYLVSHGRMKEKEARAKFRQIVSAVHYCHQKNIVHRDLKAENLLL---DSESN-------------------------IKIADFGFSNEFTPGGKLDTFCGSPPYAAPELFQGKRYNGPEVDVWSLGVILYTLVSGSLPFDGQNLKELRERVLRGKYRIPFYMSTDCEGVLRRFLVLNPSKRCTLDQIMNDKWMNIGFDSDDLKPYK 315
BLAST of Serine/threonine-protein kinase NIM1 vs. Ensembl Xenopus
Match: ENSXETT00000045413.1 (MAP/microtubule affinity-regulating kinase 4 [Source:NCBI gene;Acc:100491038]) HSP 1 Score: 261.151 bits (666), Expect = 7.533e-79 Identity = 150/434 (34.56%), Postives = 237/434 (54.61%), Query Frame = 2
Query: 236 VGLYRIKGNLGNGNFSQVKLAVHLLTREEVAIKILDKTKLDAKTQKLLSREISSMERLHHPNIIRLYEVIEDIRKMHLVMEYASGGELHSLIVSHGKIPESISKLIFAQIIAAINHLHSQNVIHRDLKAENIFFAGNKSETNQDNSKLNSTTKKSDFTDNAFSRLVHVKLGDFGFSTLAQPAEKLTTFCGSPPYAAPELFQADWYYGTCVDIWALGILLYFMLCGCMPFRGESVGLLKKLILAGNYVCPNWLPVDAKNLIQQILKQNPDQRLTIQQIKNHQWLSGQHFPDAYQPFKATPLMPEIETISHAXXXXXXXXXXXXPLEKDSIEASK--MLLRLGITDKHIEVAQAQTVRNYVTGTYRIMLHRVHRNRRMSNIPHSLSNPELGRKFFNSRKFQSKVITRKVNGAGPKPKTSSCPEKGIEPNQKSSKMC 1531
VG YR+ +G GNF++VKLA H+LT EVAIKI+DKT+L+ + + L RE+ M+ L+HPNI++L+EVIE + ++L+MEYASGGE+ +VSHG++ E ++ F QI++A+++ H +N++HRDLKAEN+ SE+N +K+ DFGFS P KL TFCGSPPYAAPELFQ Y G VD+W+LG++LY ++ G +PF G+++ L++ +L G Y P ++ D + ++++ L NP +R T+ QI N +W++ D +P+K E+D+ + + ++L +G + + I+ A + N V TY ++L R R S S+ L +S+V + NGA S ++G + ++ S C
Sbjct: 45 VGNYRLLRTIGKGNFAKVKLARHVLTGREVAIKIIDKTQLNPSSLQKLFREVRIMKGLNHPNIVKLFEVIETEKTLYLIMEYASGGEVFDYLVSHGRMKEKEARAKFRQIVSAVHYCHQKNIVHRDLKAENLLL---DSESN-------------------------IKIADFGFSNEFTPGGKLDTFCGSPPYAAPELFQGKRYNGPEVDVWSLGVILYTLVSGSLPFDGQNLKELRERVLRGKYRIPFYMSTDCEGVLRRFLVLNPSKRCTLDQIMNDKWMNIGFDSDDLKPYKEP--------------------------EEDNADPKRIEIMLEMGYSREEIKDALSSNKYNEVMATY-LLLGRKPEVRCQPIRGESRSDSTLSLS-------RSRVASDVTNGATKSSSVHSKNQRGYQRQRRHSDFC 416
BLAST of Serine/threonine-protein kinase NIM1 vs. Ensembl Xenopus
Match: ENSXETT00000045600.1 (MAP/microtubule affinity-regulating kinase 4 [Source:NCBI gene;Acc:100491038]) HSP 1 Score: 259.61 bits (662), Expect = 9.439e-79 Identity = 125/296 (42.23%), Postives = 190/296 (64.19%), Query Frame = 2
Query: 236 VGLYRIKGNLGNGNFSQVKLAVHLLTREEVAIKILDKTKLDAKTQKLLSREISSMERLHHPNIIRLYEVIEDIRKMHLVMEYASGGELHSLIVSHGKIPESISKLIFAQIIAAINHLHSQNVIHRDLKAENIFFAGNKSETNQDNSKLNSTTKKSDFTDNAFSRLVHVKLGDFGFSTLAQPAEKLTTFCGSPPYAAPELFQADWYYGTCVDIWALGILLYFMLCGCMPFRGESVGLLKKLILAGNYVCPNWLPVDAKNLIQQILKQNPDQRLTIQQIKNHQWLSGQHFPDAYQPFK 1123
VG YR+ +G GNF++VKLA H+LT EVAIKI+DKT+L+ + + L RE+ M+ L+HPNI++L+EVIE + ++L+MEYASGGE+ +VSHG++ E ++ F QI++A+++ H +N++HRDLKAEN+ SE+N +K+ DFGFS P KL TFCGSPPYAAPELFQ Y G VD+W+LG++LY ++ G +PF G+++ L++ +L G Y P ++ D + ++++ L NP +R T+ QI N +W++ D +P+K
Sbjct: 48 VGNYRLLRTIGKGNFAKVKLARHVLTGREVAIKIIDKTQLNPSSLQKLFREVRIMKGLNHPNIVKLFEVIETEKTLYLIMEYASGGEVFDYLVSHGRMKEKEARAKFRQIVSAVHYCHQKNIVHRDLKAENLLL---DSESN-------------------------IKIADFGFSNEFTPGGKLDTFCGSPPYAAPELFQGKRYNGPEVDVWSLGVILYTLVSGSLPFDGQNLKELRERVLRGKYRIPFYMSTDCEGVLRRFLVLNPSKRCTLDQIMNDKWMNIGFDSDDLKPYK 315
BLAST of Serine/threonine-protein kinase NIM1 vs. Ensembl Mouse
Match: Nim1k (NIM1 serine/threonine protein kinase [Source:MGI Symbol;Acc:MGI:2442399]) HSP 1 Score: 392.889 bits (1008), Expect = 5.666e-132 Identity = 199/415 (47.95%), Postives = 262/415 (63.13%), Query Frame = 2
Query: 128 KISETEENNVETSFDKYTEEMNCNENVKKEIMLGKRVGLYRIKGNLGNGNFSQVKLAVHLLTREEVAIKILDKTKLDAKTQKLLSREISSMERLHHPNIIRLYEVIEDIRKMHLVMEYASGGELHSLIVSHGKIPESISKLIFAQIIAAINHLHSQNVIHRDLKAENIFFAGNKSETNQDNSKLNSTTKKSDFTDNAFSRLVHVKLGDFGFSTLAQPAEKLTTFCGSPPYAAPELFQADWYYGTCVDIWALGILLYFMLCGCMPFRGESVGLLKKLILAGNYVCPNWLPVDAKNLIQQILKQNPDQRLTIQQIKNHQWLSGQHFPDAYQPFKATPL-MPEIETISHAXXXXXXXXXXXXPLEKDSIEASKMLLRLGITDKHIEVAQAQTVRNYVTGTYRIMLHRVHRNRRMSNIP 1369
K SE ++ T F+K T++M +E V +EI LGKR+G YRI+G +G+GNFSQVKL +H LT+E+VAIKILDKTKLD KTQ+LLSREISSME+LHHPNI+RLYEV+E + K+HLVMEYA GGEL I + GK+ E SKLIF+QI++A+ H+H +IHRDLKAEN+F+ VK+GDFGFST+++ E L TFCGSPPYAAPELF+ Y G VDIWALG+LLYFM+ G MPFR E+V LKK IL G Y P + LI+ +L+ P +R I I +++W+ G +P +PF+ P + E T L+++ E L LGITD+HI Q + R+ +TG YRI+LHRV R + + ++P
Sbjct: 35 KDSEEDQQRQLTPFEKLTQDMCQDEKVVREITLGKRIGFYRIRGEIGSGNFSQVKLGIHSLTKEKVAIKILDKTKLDQKTQRLLSREISSMEKLHHPNIVRLYEVVETLSKLHLVMEYAGGGELFGKISTEGKLSEPESKLIFSQILSAVKHMHENQIIHRDLKAENVFYTSRTC----------------------------VKVGDFGFSTVSKKGEMLNTFCGSPPYAAPELFRDQHYVGVYVDIWALGVLLYFMVTGTMPFRAETVAKLKKSILEGTYTIPQHVSEPCHRLIRGVLQPTPTERYGINYIMSNEWMRGVPYPTPLEPFQLDPKHLSETST-----------------LKEEENEVKSTLEHLGITDEHIRNNQGRDARSSITGVYRIILHRVQRRKALESVP 404
BLAST of Serine/threonine-protein kinase NIM1 vs. Ensembl Mouse
Match: Mark3 (MAP/microtubule affinity regulating kinase 3 [Source:MGI Symbol;Acc:MGI:1341865]) HSP 1 Score: 258.84 bits (660), Expect = 7.073e-77 Identity = 122/305 (40.00%), Postives = 192/305 (62.95%), Query Frame = 2
Query: 236 VGLYRIKGNLGNGNFSQVKLAVHLLTREEVAIKILDKTKLDAKTQKLLSREISSMERLHHPNIIRLYEVIEDIRKMHLVMEYASGGELHSLIVSHGKIPESISKLIFAQIIAAINHLHSQNVIHRDLKAENIFFAGNKSETNQDNSKLNSTTKKSDFTDNAFSRLVHVKLGDFGFSTLAQPAEKLTTFCGSPPYAAPELFQADWYYGTCVDIWALGILLYFMLCGCMPFRGESVGLLKKLILAGNYVCPNWLPVDAKNLIQQILKQNPDQRLTIQQIKNHQWLSGQHFPDAYQPFKATPLMPEIE 1150
+G YR+ +G GNF++VKLA H+LT EVAIKI+DKT+L+ + + L RE+ M+ L+HPNI++L+EVIE + ++L+MEYASGGE+ +V+HG++ E ++ F QI++A+ + H + ++HRDLKAEN+ + +++K+ DFGFS KL TFCGSPPYAAPELFQ Y G VD+W+LG++LY ++ G +PF G+++ L++ +L G Y P ++ D +NL+++ L NP +R T++QI +W++ H D +PF + PE++
Sbjct: 53 IGNYRLLKTIGKGNFAKVKLARHILTGREVAIKIIDKTQLNPTSLQKLFREVRIMKILNHPNIVKLFEVIETEKTLYLIMEYASGGEVFDYLVAHGRMKEKEARAKFRQIVSAVQYCHQKRIVHRDLKAENLLLDAD----------------------------MNIKIADFGFSNEFTVGSKLDTFCGSPPYAAPELFQGKKYDGPEVDVWSLGVILYTLVSGSLPFDGQNLKELRERVLRGKYRIPFYMSTDCENLLKRFLVLNPVKRGTLEQIMKDRWINAGHEEDELKPF----VEPELD 325
BLAST of Serine/threonine-protein kinase NIM1 vs. Ensembl Mouse
Match: Mark3 (MAP/microtubule affinity regulating kinase 3 [Source:MGI Symbol;Acc:MGI:1341865]) HSP 1 Score: 258.455 bits (659), Expect = 7.543e-77 Identity = 122/305 (40.00%), Postives = 192/305 (62.95%), Query Frame = 2
Query: 236 VGLYRIKGNLGNGNFSQVKLAVHLLTREEVAIKILDKTKLDAKTQKLLSREISSMERLHHPNIIRLYEVIEDIRKMHLVMEYASGGELHSLIVSHGKIPESISKLIFAQIIAAINHLHSQNVIHRDLKAENIFFAGNKSETNQDNSKLNSTTKKSDFTDNAFSRLVHVKLGDFGFSTLAQPAEKLTTFCGSPPYAAPELFQADWYYGTCVDIWALGILLYFMLCGCMPFRGESVGLLKKLILAGNYVCPNWLPVDAKNLIQQILKQNPDQRLTIQQIKNHQWLSGQHFPDAYQPFKATPLMPEIE 1150
+G YR+ +G GNF++VKLA H+LT EVAIKI+DKT+L+ + + L RE+ M+ L+HPNI++L+EVIE + ++L+MEYASGGE+ +V+HG++ E ++ F QI++A+ + H + ++HRDLKAEN+ + +++K+ DFGFS KL TFCGSPPYAAPELFQ Y G VD+W+LG++LY ++ G +PF G+++ L++ +L G Y P ++ D +NL+++ L NP +R T++QI +W++ H D +PF + PE++
Sbjct: 53 IGNYRLLKTIGKGNFAKVKLARHILTGREVAIKIIDKTQLNPTSLQKLFREVRIMKILNHPNIVKLFEVIETEKTLYLIMEYASGGEVFDYLVAHGRMKEKEARAKFRQIVSAVQYCHQKRIVHRDLKAENLLLDAD----------------------------MNIKIADFGFSNEFTVGSKLDTFCGSPPYAAPELFQGKKYDGPEVDVWSLGVILYTLVSGSLPFDGQNLKELRERVLRGKYRIPFYMSTDCENLLKRFLVLNPVKRGTLEQIMKDRWINAGHEEDELKPF----VEPELD 325
BLAST of Serine/threonine-protein kinase NIM1 vs. Ensembl Mouse
Match: Mark3 (MAP/microtubule affinity regulating kinase 3 [Source:MGI Symbol;Acc:MGI:1341865]) HSP 1 Score: 258.84 bits (660), Expect = 9.823e-77 Identity = 122/305 (40.00%), Postives = 192/305 (62.95%), Query Frame = 2
Query: 236 VGLYRIKGNLGNGNFSQVKLAVHLLTREEVAIKILDKTKLDAKTQKLLSREISSMERLHHPNIIRLYEVIEDIRKMHLVMEYASGGELHSLIVSHGKIPESISKLIFAQIIAAINHLHSQNVIHRDLKAENIFFAGNKSETNQDNSKLNSTTKKSDFTDNAFSRLVHVKLGDFGFSTLAQPAEKLTTFCGSPPYAAPELFQADWYYGTCVDIWALGILLYFMLCGCMPFRGESVGLLKKLILAGNYVCPNWLPVDAKNLIQQILKQNPDQRLTIQQIKNHQWLSGQHFPDAYQPFKATPLMPEIE 1150
+G YR+ +G GNF++VKLA H+LT EVAIKI+DKT+L+ + + L RE+ M+ L+HPNI++L+EVIE + ++L+MEYASGGE+ +V+HG++ E ++ F QI++A+ + H + ++HRDLKAEN+ + +++K+ DFGFS KL TFCGSPPYAAPELFQ Y G VD+W+LG++LY ++ G +PF G+++ L++ +L G Y P ++ D +NL+++ L NP +R T++QI +W++ H D +PF + PE++
Sbjct: 53 IGNYRLLKTIGKGNFAKVKLARHILTGREVAIKIIDKTQLNPTSLQKLFREVRIMKILNHPNIVKLFEVIETEKTLYLIMEYASGGEVFDYLVAHGRMKEKEARAKFRQIVSAVQYCHQKRIVHRDLKAENLLLDAD----------------------------MNIKIADFGFSNEFTVGSKLDTFCGSPPYAAPELFQGKKYDGPEVDVWSLGVILYTLVSGSLPFDGQNLKELRERVLRGKYRIPFYMSTDCENLLKRFLVLNPVKRGTLEQIMKDRWINAGHEEDELKPF----VEPELD 325
BLAST of Serine/threonine-protein kinase NIM1 vs. Ensembl Mouse
Match: Mark2 (MAP/microtubule affinity regulating kinase 2 [Source:MGI Symbol;Acc:MGI:99638]) HSP 1 Score: 257.299 bits (656), Expect = 2.159e-76 Identity = 119/295 (40.34%), Postives = 189/295 (64.07%), Query Frame = 2
Query: 236 VGLYRIKGNLGNGNFSQVKLAVHLLTREEVAIKILDKTKLDAKTQKLLSREISSMERLHHPNIIRLYEVIEDIRKMHLVMEYASGGELHSLIVSHGKIPESISKLIFAQIIAAINHLHSQNVIHRDLKAENIFFAGNKSETNQDNSKLNSTTKKSDFTDNAFSRLVHVKLGDFGFSTLAQPAEKLTTFCGSPPYAAPELFQADWYYGTCVDIWALGILLYFMLCGCMPFRGESVGLLKKLILAGNYVCPNWLPVDAKNLIQQILKQNPDQRLTIQQIKNHQWLSGQHFPDAYQPF 1120
+G YR+ +G GNF++VKLA H+LT +EVA+KI+DKT+L++ + + L RE+ M+ L+HPNI++L+EVIE + ++LVMEYASGGE+ +V+HG++ E ++ F QI++A+ + H + ++HRDLKAEN+ + +++K+ DFGFS KL TFCGSPPYAAPELFQ Y G VD+W+LG++LY ++ G +PF G+++ L++ +L G Y P ++ D +NL+++ L NP +R T++QI +W++ H D +P+
Sbjct: 50 IGNYRLLKTIGKGNFAKVKLARHILTGKEVAVKIIDKTQLNSSSLQKLFREVRIMKVLNHPNIVKLFEVIETEKTLYLVMEYASGGEVFDYLVAHGRMKEKEARAKFRQIVSAVQYCHQKFIVHRDLKAENLLLDAD----------------------------MNIKIADFGFSNEFTFGNKLDTFCGSPPYAAPELFQGKKYDGPEVDVWSLGVILYTLVSGSLPFDGQNLKELRERVLRGKYRIPFYMSTDCENLLKKFLILNPSKRGTLEQIMKDRWMNVGHEDDELKPY 316
BLAST of Serine/threonine-protein kinase NIM1 vs. UniProt/SwissProt
Match: sp|Q8IY84|NIM1_HUMAN (Serine/threonine-protein kinase NIM1 OS=Homo sapiens OX=9606 GN=NIM1K PE=1 SV=1) HSP 1 Score: 395.201 bits (1014), Expect = 6.018e-132 Identity = 199/404 (49.26%), Postives = 260/404 (64.36%), Query Frame = 2
Query: 161 TSFDKYTEEMNCNENVKKEIMLGKRVGLYRIKGNLGNGNFSQVKLAVHLLTREEVAIKILDKTKLDAKTQKLLSREISSMERLHHPNIIRLYEVIEDIRKMHLVMEYASGGELHSLIVSHGKIPESISKLIFAQIIAAINHLHSQNVIHRDLKAENIFFAGNKSETNQDNSKLNSTTKKSDFTDNAFSRLVHVKLGDFGFSTLAQPAEKLTTFCGSPPYAAPELFQADWYYGTCVDIWALGILLYFMLCGCMPFRGESVGLLKKLILAGNYVCPNWLPVDAKNLIQQILKQNPDQRLTIQQIKNHQWLSGQHFPDAYQPFKATPL-MPEIETISHAXXXXXXXXXXXXPLEKDSIEASKMLLRLGITDKHIEVAQAQTVRNYVTGTYRIMLHRVHRNRRMSNIP 1369
T F+K T++M+ +E V +EI LGKR+G YRI+G +G+GNFSQVKL +H LT+E+VAIKILDKTKLD KTQ+LLSREISSME+LHHPNIIRLYEV+E + K+HLVMEYA GGEL I + GK+ E SKLIF+QI++A+ H+H +IHRDLKAEN+F+ N VK+GDFGFST+++ E L TFCGSPPYAAPELF+ + Y G VDIWALG+LLYFM+ G MPFR E+V LKK IL G Y P + LI+ +L+Q P +R I I N +W+ G +P +PF+ P + E T L+++ E L LGIT++HI Q + R+ +TG YRI+LHRV R + + ++P
Sbjct: 46 TPFEKLTQDMSQDEKVVREITLGKRIGFYRIRGEIGSGNFSQVKLGIHSLTKEKVAIKILDKTKLDQKTQRLLSREISSMEKLHHPNIIRLYEVVETLSKLHLVMEYAGGGELFGKISTEGKLSEPESKLIFSQIVSAVKHMHENQIIHRDLKAENVFYTSNTC----------------------------VKVGDFGFSTVSKKGEMLNTFCGSPPYAAPELFRDEHYIGIYVDIWALGVLLYFMVTGTMPFRAETVAKLKKSILEGTYSVPPHVSEPCHRLIRGVLQQIPTERYGIDCIMNDEWMQGVPYPTPLEPFQLDPKHLSETST-----------------LKEEENEVKSTLEHLGITEEHIRNNQGRDARSSITGVYRIILHRVQRKKALESVP 404
BLAST of Serine/threonine-protein kinase NIM1 vs. UniProt/SwissProt
Match: sp|Q8BHI9|NIM1_MOUSE (Serine/threonine-protein kinase NIM1 OS=Mus musculus OX=10090 GN=Nim1k PE=2 SV=1) HSP 1 Score: 392.889 bits (1008), Expect = 3.964e-131 Identity = 199/415 (47.95%), Postives = 262/415 (63.13%), Query Frame = 2
Query: 128 KISETEENNVETSFDKYTEEMNCNENVKKEIMLGKRVGLYRIKGNLGNGNFSQVKLAVHLLTREEVAIKILDKTKLDAKTQKLLSREISSMERLHHPNIIRLYEVIEDIRKMHLVMEYASGGELHSLIVSHGKIPESISKLIFAQIIAAINHLHSQNVIHRDLKAENIFFAGNKSETNQDNSKLNSTTKKSDFTDNAFSRLVHVKLGDFGFSTLAQPAEKLTTFCGSPPYAAPELFQADWYYGTCVDIWALGILLYFMLCGCMPFRGESVGLLKKLILAGNYVCPNWLPVDAKNLIQQILKQNPDQRLTIQQIKNHQWLSGQHFPDAYQPFKATPL-MPEIETISHAXXXXXXXXXXXXPLEKDSIEASKMLLRLGITDKHIEVAQAQTVRNYVTGTYRIMLHRVHRNRRMSNIP 1369
K SE ++ T F+K T++M +E V +EI LGKR+G YRI+G +G+GNFSQVKL +H LT+E+VAIKILDKTKLD KTQ+LLSREISSME+LHHPNI+RLYEV+E + K+HLVMEYA GGEL I + GK+ E SKLIF+QI++A+ H+H +IHRDLKAEN+F+ VK+GDFGFST+++ E L TFCGSPPYAAPELF+ Y G VDIWALG+LLYFM+ G MPFR E+V LKK IL G Y P + LI+ +L+ P +R I I +++W+ G +P +PF+ P + E T L+++ E L LGITD+HI Q + R+ +TG YRI+LHRV R + + ++P
Sbjct: 35 KDSEEDQQRQLTPFEKLTQDMCQDEKVVREITLGKRIGFYRIRGEIGSGNFSQVKLGIHSLTKEKVAIKILDKTKLDQKTQRLLSREISSMEKLHHPNIVRLYEVVETLSKLHLVMEYAGGGELFGKISTEGKLSEPESKLIFSQILSAVKHMHENQIIHRDLKAENVFYTSRTC----------------------------VKVGDFGFSTVSKKGEMLNTFCGSPPYAAPELFRDQHYVGVYVDIWALGVLLYFMVTGTMPFRAETVAKLKKSILEGTYTIPQHVSEPCHRLIRGVLQPTPTERYGINYIMSNEWMRGVPYPTPLEPFQLDPKHLSETST-----------------LKEEENEVKSTLEHLGITDEHIRNNQGRDARSSITGVYRIILHRVQRRKALESVP 404
BLAST of Serine/threonine-protein kinase NIM1 vs. UniProt/SwissProt
Match: sp|Q03141|MARK3_MOUSE (MAP/microtubule affinity-regulating kinase 3 OS=Mus musculus OX=10090 GN=Mark3 PE=1 SV=2) HSP 1 Score: 258.84 bits (660), Expect = 6.871e-76 Identity = 122/305 (40.00%), Postives = 192/305 (62.95%), Query Frame = 2
Query: 236 VGLYRIKGNLGNGNFSQVKLAVHLLTREEVAIKILDKTKLDAKTQKLLSREISSMERLHHPNIIRLYEVIEDIRKMHLVMEYASGGELHSLIVSHGKIPESISKLIFAQIIAAINHLHSQNVIHRDLKAENIFFAGNKSETNQDNSKLNSTTKKSDFTDNAFSRLVHVKLGDFGFSTLAQPAEKLTTFCGSPPYAAPELFQADWYYGTCVDIWALGILLYFMLCGCMPFRGESVGLLKKLILAGNYVCPNWLPVDAKNLIQQILKQNPDQRLTIQQIKNHQWLSGQHFPDAYQPFKATPLMPEIE 1150
+G YR+ +G GNF++VKLA H+LT EVAIKI+DKT+L+ + + L RE+ M+ L+HPNI++L+EVIE + ++L+MEYASGGE+ +V+HG++ E ++ F QI++A+ + H + ++HRDLKAEN+ + +++K+ DFGFS KL TFCGSPPYAAPELFQ Y G VD+W+LG++LY ++ G +PF G+++ L++ +L G Y P ++ D +NL+++ L NP +R T++QI +W++ H D +PF + PE++
Sbjct: 53 IGNYRLLKTIGKGNFAKVKLARHILTGREVAIKIIDKTQLNPTSLQKLFREVRIMKILNHPNIVKLFEVIETEKTLYLIMEYASGGEVFDYLVAHGRMKEKEARAKFRQIVSAVQYCHQKRIVHRDLKAENLLLDAD----------------------------MNIKIADFGFSNEFTVGSKLDTFCGSPPYAAPELFQGKKYDGPEVDVWSLGVILYTLVSGSLPFDGQNLKELRERVLRGKYRIPFYMSTDCENLLKRFLVLNPVKRGTLEQIMKDRWINAGHEEDELKPF----VEPELD 325
BLAST of Serine/threonine-protein kinase NIM1 vs. UniProt/SwissProt
Match: sp|P27448|MARK3_HUMAN (MAP/microtubule affinity-regulating kinase 3 OS=Homo sapiens OX=9606 GN=MARK3 PE=1 SV=5) HSP 1 Score: 258.07 bits (658), Expect = 1.049e-75 Identity = 122/305 (40.00%), Postives = 192/305 (62.95%), Query Frame = 2
Query: 236 VGLYRIKGNLGNGNFSQVKLAVHLLTREEVAIKILDKTKLDAKTQKLLSREISSMERLHHPNIIRLYEVIEDIRKMHLVMEYASGGELHSLIVSHGKIPESISKLIFAQIIAAINHLHSQNVIHRDLKAENIFFAGNKSETNQDNSKLNSTTKKSDFTDNAFSRLVHVKLGDFGFSTLAQPAEKLTTFCGSPPYAAPELFQADWYYGTCVDIWALGILLYFMLCGCMPFRGESVGLLKKLILAGNYVCPNWLPVDAKNLIQQILKQNPDQRLTIQQIKNHQWLSGQHFPDAYQPFKATPLMPEIE 1150
+G YR+ +G GNF++VKLA H+LT EVAIKI+DKT+L+ + + L RE+ M+ L+HPNI++L+EVIE + ++L+MEYASGGE+ +V+HG++ E ++ F QI++A+ + H + ++HRDLKAEN+ + +++K+ DFGFS KL TFCGSPPYAAPELFQ Y G VD+W+LG++LY ++ G +PF G+++ L++ +L G Y P ++ D +NL+++ L NP +R T++QI +W++ H D +PF + PE++
Sbjct: 53 IGNYRLLKTIGKGNFAKVKLARHILTGREVAIKIIDKTQLNPTSLQKLFREVRIMKILNHPNIVKLFEVIETEKTLYLIMEYASGGEVFDYLVAHGRMKEKEARSKFRQIVSAVQYCHQKRIVHRDLKAENLLLDAD----------------------------MNIKIADFGFSNEFTVGGKLDTFCGSPPYAAPELFQGKKYDGPEVDVWSLGVILYTLVSGSLPFDGQNLKELRERVLRGKYRIPFYMSTDCENLLKRFLVLNPIKRGTLEQIMKDRWINAGHEEDELKPF----VEPELD 325
BLAST of Serine/threonine-protein kinase NIM1 vs. UniProt/SwissProt
Match: sp|O08679|MARK2_RAT (Serine/threonine-protein kinase MARK2 OS=Rattus norvegicus OX=10116 GN=Mark2 PE=1 SV=1) HSP 1 Score: 256.914 bits (655), Expect = 1.973e-75 Identity = 119/295 (40.34%), Postives = 189/295 (64.07%), Query Frame = 2
Query: 236 VGLYRIKGNLGNGNFSQVKLAVHLLTREEVAIKILDKTKLDAKTQKLLSREISSMERLHHPNIIRLYEVIEDIRKMHLVMEYASGGELHSLIVSHGKIPESISKLIFAQIIAAINHLHSQNVIHRDLKAENIFFAGNKSETNQDNSKLNSTTKKSDFTDNAFSRLVHVKLGDFGFSTLAQPAEKLTTFCGSPPYAAPELFQADWYYGTCVDIWALGILLYFMLCGCMPFRGESVGLLKKLILAGNYVCPNWLPVDAKNLIQQILKQNPDQRLTIQQIKNHQWLSGQHFPDAYQPF 1120
+G YR+ +G GNF++VKLA H+LT +EVA+KI+DKT+L++ + + L RE+ M+ L+HPNI++L+EVIE + ++LVMEYASGGE+ +V+HG++ E ++ F QI++A+ + H + ++HRDLKAEN+ + +++K+ DFGFS KL TFCGSPPYAAPELFQ Y G VD+W+LG++LY ++ G +PF G+++ L++ +L G Y P ++ D +NL+++ L NP +R T++QI +W++ H D +P+
Sbjct: 50 IGNYRLLKTIGKGNFAKVKLARHILTGKEVAVKIIDKTQLNSSSLQKLFREVRIMKVLNHPNIVKLFEVIETEKTLYLVMEYASGGEVFDYLVAHGRMKEKEARAKFRQIVSAVQYCHQKFIVHRDLKAENLLLDAD----------------------------MNIKIADFGFSNEFTFGNKLDTFCGSPPYAAPELFQGKKYDGPEVDVWSLGVILYTLVSGSLPFDGQNLKELRERVLRGKYRIPFYMSTDCENLLKKFLILNPSKRGTLEQIMKDRWMNVGHEDDELKPY 316
BLAST of Serine/threonine-protein kinase NIM1 vs. TrEMBL
Match: A0A1I8HRE9 (Protein kinase domain-containing protein OS=Macrostomum lignano OX=282301 PE=3 SV=1) HSP 1 Score: 410.609 bits (1054), Expect = 6.938e-135 Identity = 201/395 (50.89%), Postives = 264/395 (66.84%), Query Frame = 2
Query: 161 TSFDKYTEEMNCNENVKKEIMLGKRVGLYRIKGNLGNGNFSQVKLAVHLLTREEVAIKILDKTKLDAKTQKLLSREISSMERLHHPNIIRLYEVIEDIRKMHLVMEYASGGELHSLIVSHGKIPESISKLIFAQIIAAINHLHSQNVIHRDLKAENIFFAGNKSETNQDNSKLNSTTKKSDFTDNAFSRLVHVKLGDFGFSTLAQPAEKLTTFCGSPPYAAPELFQADWYYGTCVDIWALGILLYFMLCGCMPFRGESVGLLKKLILAGNYVCPNWLPVDAKNLIQQILKQNPDQRLTIQQIKNHQWLSGQHFPDAYQPFKATPLMPEIETISHAXXXXXXXXXXXXPLEKDSIEASKMLLRLGITDKHIEVAQAQTVRNYVTGTYRIMLHRVHR 1345
T+++K T E+ ++NV+KEI LG+R+G YRI+G LG GNFSQVKLA H LT+E+VAIKILDKTKLDAKTQ+LLSRE+S MERLHHP +IRLYEV+E + K+H+VMEYA GGEL + I S G+ E +++IF+Q+ +AI H+H NVIHRDLKAEN+FF+G HVK+GDFGFSTLA+P ++L TFCGSPPYAAPELF+ D Y G VDIWALGI+LYFML G MPFR ++VG LKKLILAG Y P +L A+ LI IL+ +P R T+ I++ WL G+ F A + ++ P ++ L + +E K L +LGI++ +E A ++ R+ +TGTYRI LH+VH+
Sbjct: 102 TAYEKLTFELENDDNVQKEITLGRRIGFYRIRGELGTGNFSQVKLATHALTKEKVAIKILDKTKLDAKTQRLLSREVSVMERLHHPCVIRLYEVMESVAKIHIVMEYAPGGELFTKITSEGRFSERHARVIFSQVASAIKHMHDHNVIHRDLKAENVFFSGPS----------------------------HVKVGDFGFSTLAKPQQELNTFCGSPPYAAPELFRDDSYQGPRVDIWALGIMLYFMLTGLMPFRADTVGRLKKLILAGEYSMPAFLSEPAQALIASILRLSPPDRATMSDIQSSAWLEGEEFLPALEKYEMNP----------------RRVANRADLRPEELEVRKNLQQLGISESLLEEASGKSYRSSITGTYRIALHKVHK 452
BLAST of Serine/threonine-protein kinase NIM1 vs. TrEMBL
Match: A0A267H059 (Protein kinase domain-containing protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig007816g1 PE=4 SV=1) HSP 1 Score: 410.994 bits (1055), Expect = 1.899e-134 Identity = 201/395 (50.89%), Postives = 264/395 (66.84%), Query Frame = 2
Query: 161 TSFDKYTEEMNCNENVKKEIMLGKRVGLYRIKGNLGNGNFSQVKLAVHLLTREEVAIKILDKTKLDAKTQKLLSREISSMERLHHPNIIRLYEVIEDIRKMHLVMEYASGGELHSLIVSHGKIPESISKLIFAQIIAAINHLHSQNVIHRDLKAENIFFAGNKSETNQDNSKLNSTTKKSDFTDNAFSRLVHVKLGDFGFSTLAQPAEKLTTFCGSPPYAAPELFQADWYYGTCVDIWALGILLYFMLCGCMPFRGESVGLLKKLILAGNYVCPNWLPVDAKNLIQQILKQNPDQRLTIQQIKNHQWLSGQHFPDAYQPFKATPLMPEIETISHAXXXXXXXXXXXXPLEKDSIEASKMLLRLGITDKHIEVAQAQTVRNYVTGTYRIMLHRVHR 1345
T+++K T E+ ++NV+KEI LG+R+G YRI+G LG GNFSQVKLA H LT+E+VAIKILDKTKLDAKTQ+LLSRE+S MERLHHP +IRLYEV+E + K+H+VMEYA GGEL + I S G+ E +++IF+Q+ +AI H+H NVIHRDLKAEN+FF+G HVK+GDFGFSTLA+P ++L TFCGSPPYAAPELF+ D Y G VDIWALGI+LYFML G MPFR ++VG LKKLILAG Y P +L A+ LI IL+ +P R T+ I++ WL G+ F A + ++ P ++ L + +E K L +LGI++ +E A ++ R+ +TGTYRI LH+VH+
Sbjct: 111 TAYEKLTFELENDDNVQKEITLGRRIGFYRIRGELGTGNFSQVKLATHALTKEKVAIKILDKTKLDAKTQRLLSREVSVMERLHHPCVIRLYEVMESVAKIHIVMEYAPGGELFTKITSEGRFSERHARVIFSQVASAIKHMHDHNVIHRDLKAENVFFSGPS----------------------------HVKVGDFGFSTLAKPQQELNTFCGSPPYAAPELFRDDSYQGPRVDIWALGIMLYFMLTGLMPFRADTVGRLKKLILAGEYSMPAFLSEPAQALIASILRLSPPDRATMSDIQSSAWLEGEEFLPALEKYEMNP----------------RRVANRADLRPEELEVRKNLQQLGISESLLEEASGKSYRSSITGTYRIALHKVHK 461
BLAST of Serine/threonine-protein kinase NIM1 vs. TrEMBL
Match: V4ABV4 (Protein kinase domain-containing protein OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_216647 PE=3 SV=1) HSP 1 Score: 405.986 bits (1042), Expect = 1.129e-133 Identity = 195/395 (49.37%), Postives = 271/395 (68.61%), Query Frame = 2
Query: 161 TSFDKYTEEMNCNENVKKEIMLGKRVGLYRIKGNLGNGNFSQVKLAVHLLTREEVAIKILDKTKLDAKTQKLLSREISSMERLHHPNIIRLYEVIEDIRKMHLVMEYASGGELHSLIVSHGKIPESISKLIFAQIIAAINHLHSQNVIHRDLKAENIFFAGNKSETNQDNSKLNSTTKKSDFTDNAFSRLVHVKLGDFGFSTLAQPAEKLTTFCGSPPYAAPELFQADWYYGTCVDIWALGILLYFMLCGCMPFRGESVGLLKKLILAGNYVCPNWLPVDAKNLIQQILKQNPDQRLTIQQIKNHQWLSGQHFPDAYQPFKATPLMPEIETISHAXXXXXXXXXXXXPLEKDSIEASKMLLRLGITDKHIEVAQAQTVRNYVTGTYRIMLHRVHR 1345
T ++ T ++N + KEI LGKR+G YRI+G LG+GNFSQVK+ +H LTRE+VAIKILDKTKLD KTQ+LLSREI++M++LHHPNIIRLYEV+E + K+H+VMEYA GGE+ + I + GK+ E+ SKL+F+Q+IAA+ H+H QN+IHRDLKAEN+F+A V VK+GDFGFST+++P + L+TFCGSPPYAAPELF+ + Y+G+ VDIWALGI+LYFM+ G MPFR E+V LKK IL G+Y P+++ + LI+ +LK P R TI +IK +WL Q+FP A++P+ P P+ ++ L K+ EA ++L LGIT++H+ + +Q R+ +TG+YRI+LHR+ +
Sbjct: 48 TPLERLTHDLNNDPKCTKEITLGKRIGFYRIRGELGSGNFSQVKMGIHALTREKVAIKILDKTKLDQKTQRLLSREIANMDKLHHPNIIRLYEVVETLAKLHMVMEYACGGEVFTKISNEGKLSEAESKLLFSQVIAAVEHMHEQNIIHRDLKAENLFYASP----------------------------VLVKVGDFGFSTISRPDQTLSTFCGSPPYAAPELFKDESYFGSYVDIWALGIMLYFMVTGIMPFRAETVAKLKKCILDGSYTIPSYISDSCQFLIRSMLKHIPHDRFTITEIKRSEWLRDQNFPQAFEPYNLNP-TPQNQS---------------KELSKEESEARQVLKDLGITEQHLLNSNSQESRSSITGSYRIVLHRIQK 398
BLAST of Serine/threonine-protein kinase NIM1 vs. TrEMBL
Match: A0A151MX25 (Serine/threonine-protein kinase NIM1 OS=Alligator mississippiensis OX=8496 GN=NIM1K PE=3 SV=1) HSP 1 Score: 404.445 bits (1038), Expect = 1.385e-133 Identity = 201/404 (49.75%), Postives = 265/404 (65.59%), Query Frame = 2
Query: 161 TSFDKYTEEMNCNENVKKEIMLGKRVGLYRIKGNLGNGNFSQVKLAVHLLTREEVAIKILDKTKLDAKTQKLLSREISSMERLHHPNIIRLYEVIEDIRKMHLVMEYASGGELHSLIVSHGKIPESISKLIFAQIIAAINHLHSQNVIHRDLKAENIFFAGNKSETNQDNSKLNSTTKKSDFTDNAFSRLVHVKLGDFGFSTLAQPAEKLTTFCGSPPYAAPELFQADWYYGTCVDIWALGILLYFMLCGCMPFRGESVGLLKKLILAGNYVCPNWLPVDAKNLIQQILKQNPDQRLTIQQIKNHQWLSGQHFPDAYQPFKATP-LMPEIETISHAXXXXXXXXXXXXPLEKDSIEASKMLLRLGITDKHIEVAQAQTVRNYVTGTYRIMLHRVHRNRRMSNIP 1369
T F+K T++M NE V KE+ LGKR+G YR++G +G+GNFSQVKL +H LT+E+VAIKILDKTKLD KTQ+LLSREISSME+LHHPNIIRLYEV+E + K+HLVMEYA+GGEL S I + GK+PE SKLIF+Q+++A+ H+H N+IHRDLKAEN+F+ N VK+GDFGFST+++ E L TFCGSPPYAAPELF+ + Y G VDIWALGILLYFM+ G MPFR ++V LKK IL G Y P L + LI+ +L+ P +R +IQ I + +W+ G +P +PFK P + E +T L+++ E +L LGIT +HI + + R+ +TG YRI+LHRV + R + +P
Sbjct: 22 TPFEKLTQDMTQNEKVVKELTLGKRIGFYRVRGEIGSGNFSQVKLGIHSLTKEKVAIKILDKTKLDQKTQRLLSREISSMEKLHHPNIIRLYEVVETLSKLHLVMEYAAGGELFSKISTEGKLPEMESKLIFSQVVSAVKHMHENNIIHRDLKAENVFYTSNTC----------------------------VKVGDFGFSTISKREETLNTFCGSPPYAAPELFRDENYVGVYVDIWALGILLYFMVTGTMPFRADTVARLKKCILDGTYSLPPSLSEPCQKLIRGVLQPVPTERYSIQLIMSSEWMQGTWYPQPLEPFKLDPKYLSETDT-----------------LKEEETEVKSILEELGITQEHIHNNRGRDARSSITGIYRIVLHRVQKKRALECVP 380
BLAST of Serine/threonine-protein kinase NIM1 vs. TrEMBL
Match: A0A093JI57 (Serine/threonine-protein kinase NIM1 OS=Struthio camelus australis OX=441894 GN=N308_14983 PE=3 SV=1) HSP 1 Score: 404.445 bits (1038), Expect = 3.412e-133 Identity = 203/411 (49.39%), Postives = 270/411 (65.69%), Query Frame = 2
Query: 161 TSFDKYTEEMNCNENVKKEIMLGKRVGLYRIKGNLGNGNFSQVKLAVHLLTREEVAIKILDKTKLDAKTQKLLSREISSMERLHHPNIIRLYEVIEDIRKMHLVMEYASGGELHSLIVSHGKIPESISKLIFAQIIAAINHLHSQNVIHRDLKAENIFFAGNKSETNQDNSKLNSTTKKSDFTDNAFSRLVHVKLGDFGFSTLAQPAEKLTTFCGSPPYAAPELFQADWYYGTCVDIWALGILLYFMLCGCMPFRGESVGLLKKLILAGNYVCPNWLPVDAKNLIQQILKQNPDQRLTIQQIKNHQWLSGQHFPDAYQPFKATP-LMPEIETISHAXXXXXXXXXXXXPLEKDSIEASKMLLRLGITDKHIEVAQAQTVRNYVTGTYRIMLHRVHRNRRMSNIPHSLSNPE 1390
T F+K T +M+ NE V KE+ LGKR+G YR++G +G+GNFSQVKL +H LT+E+VAIKILDKTKLD KTQ+LLSREISSME+LHHPNIIRLYEV+E + K+HLVMEYA GGEL + I + GK+ E+ KLIF+QI++A+ H+H N+IHRDLKAEN+F+ N VK+GDFGFST++ E L TFCGSPPYAAPELF+ + Y G VDIWALGILLYFM+ G MPFR ++V LKK IL G Y P L + LI+ +L+ P R +++ I N +W+ G +P +PFK P + EI T L+++ +E +L LGIT++HI+ + + R+ VTG YRI+LHRV + R + ++P +SNP+
Sbjct: 46 TPFEKLTRDMSQNEKVVKELTLGKRIGFYRVRGEIGSGNFSQVKLGIHSLTKEKVAIKILDKTKLDQKTQRLLSREISSMEKLHHPNIIRLYEVVETLSKLHLVMEYAGGGELFTKISTEGKLLETECKLIFSQIVSAVKHMHENNIIHRDLKAENVFYTSNTC----------------------------VKVGDFGFSTISTRDETLNTFCGSPPYAAPELFRDENYVGIYVDIWALGILLYFMITGIMPFRADTVAKLKKCILEGTYSLPPSLSETCQKLIKGVLQPVPTDRYSVKLIMNSEWMQGIQYPKPLEPFKLDPKYLSEIST-----------------LKEEEVEVKSILKDLGITEEHIQNNRGKDARSSVTGVYRIVLHRVQKKRALESVP-IMSNPD 410
BLAST of Serine/threonine-protein kinase NIM1 vs. Ensembl Cavefish
Match: nim1k (NIM1 serine/threonine protein kinase [Source:ZFIN;Acc:ZDB-GENE-130530-744]) HSP 1 Score: 383.259 bits (983), Expect = 3.264e-128 Identity = 193/431 (44.78%), Postives = 271/431 (62.88%), Query Frame = 2
Query: 161 TSFDKYTEEMNCNENVKKEIMLGKRVGLYRIKGNLGNGNFSQVKLAVHLLTREEVAIKILDKTKLDAKTQKLLSREISSMERLHHPNIIRLYEVIEDIRKMHLVMEYASGGELHSLIVSHGKIPESISKLIFAQIIAAINHLHSQNVIHRDLKAENIFFAGNKSETNQDNSKLNSTTKKSDFTDNAFSRLVHVKLGDFGFSTLAQPAEKLTTFCGSPPYAAPELFQADWYYGTCVDIWALGILLYFMLCGCMPFRGESVGLLKKLILAGNYVCPNWLPVDAKNLIQQILKQNPDQRLTIQQIKNHQWLSGQHFPDAYQPFKATPLMPEIETISHAXXXXXXXXXXXXPLEKDSIEASKMLLRLGITDKHIEVAQAQTVRNYVTGTYRIMLHRVHRNRRMSNIP---HSLSNPELGR---KFFNSRKFQSKV 1435
T +K +M ++ V +E+ +G+R+G Y+I+G +G GNFS VKL +H LT++ VAIKILDKTKLD KTQKLLSREISSME+LHHPNIIRLYEV+E + ++HLVMEYA GGEL++ I GK+ + K++F+Q+++A+ H+H N+IHRDLKAEN+F+ + VK+GDFGFST+++ E L TFCGSPPYAAPELF+ Y G VD+WALG++L+F++ G MPFR E+V LK+ IL G YV P W+P + LI+ IL+ P +R +++Q+ +WL FP A +PFK PL ++ LE+D IE L LGIT +HI Q + R+ +TG YRI+LHR H+ R M ++P H ++ E+ + K + S + SK+
Sbjct: 45 TPLEKLNLDMCQDDKVVREMAVGRRIGFYKIRGEIGCGNFSHVKLGIHALTKK-VAIKILDKTKLDQKTQKLLSREISSMEKLHHPNIIRLYEVVETLTRLHLVMEYAGGGELYTKITMEGKLSDIDCKIVFSQVLSAVKHMHDNNIIHRDLKAENVFYTCSTC----------------------------VKVGDFGFSTVSRRDETLNTFCGSPPYAAPELFRDQHYTGVHVDVWALGVMLFFIVTGTMPFRAETVAKLKRCILEGAYVLPTWVPEPCQKLIRAILQTQPSERCSVEQMMACEWLQPVDFPKAVEPFKLDPLYLAESGLAE--------------LEEDEIEVRNALETLGITAEHIRNNQGKDCRSSITGVYRILLHRAHKRRAMESMPVVTHVVNGTEVKKERLKAYRSLRHTSKL 432
BLAST of Serine/threonine-protein kinase NIM1 vs. Ensembl Cavefish
Match: ENSAMXT00000032316.1 (serine/threonine-protein kinase NIM1-like [Source:NCBI gene;Acc:103045418]) HSP 1 Score: 344.354 bits (882), Expect = 4.186e-113 Identity = 179/430 (41.63%), Postives = 257/430 (59.77%), Query Frame = 2
Query: 143 EENNVETSFDKYTEEMNCNENVKKEIMLGKRVGLYRIKGNLGNGNFSQVKLAVHLLTREEVAIKILDKTKLDAKTQKLLSREISSMERLHHPNIIRLYEVIEDIRKMHLVMEYASGGELHSLIVSHGKIPESISKLIFAQIIAAINHLHSQNVIHRDLKAENIFFAGNKSETNQDNSKLNSTTKKSDFTDNAFSRLVHVKLGDFGFSTLAQPAEKLTTFCGSPPYAAPELFQADWYYGTCVDIWALGILLYFMLCGCMPFRGESVGLLKKLILAGNYVCPNWLPVDAKNLIQQILKQNPDQRLTIQQIKNHQWLSGQHFPDAYQPFKATPLMPEIETISHAXXXXXXXXXXXXPLEKDSIEASKMLLRLGITDKHIEVAQAQTVRNYVTGTYRIMLHRVHRNRRMSNIPHSLSNPELGRKFFNSRKFQSK 1432
E+ +T F++ +M NE V +I+LGKR+ Y ++G +G GNFSQVKL +H LT+E VA+KIL+K +LD KTQ L + EIS MERL HPNI+RLYEVIE + ++L MEY SGG+L S I + G++P+ SKL+FAQII+A+ ++H +++HRDLKAENIF+ + +K+GDFGFST++ P + LTTFCGSPPYAAPELF+ Y G VDIWALG+LLYFM+ MPF +++ LK+ IL G+Y P +P + I+ +L+ P R ++ Q+ + WLSG +P Y TP +H + S D L + E + L LGI+ H++ +R+ +TGTYRI+LH + + R + + +S PE F +S+ + SK
Sbjct: 47 EQRMKQTRFERVVYDMAHNERVVSDIILGKRISFYELRGEIGTGNFSQVKLGIHDLTKERVAVKILNKMRLDKKTQTLFTSEISCMERLSHPNIVRLYEVIESSKHLYLAMEYGSGGDLFSRISTRGRLPDLESKLVFAQIISAVKYMHDNDIVHRDLKAENIFYTTSYC----------------------------IKVGDFGFSTISGPGDILTTFCGSPPYAAPELFREKSYVGLFVDIWALGVLLYFMVTATMPFHADNLSRLKRCILQGSYAVPQHVPEPCQQAIKGMLRPVPPDRSSLSQLMSCSWLSGIDYPKPYPTLSLTP--------NHLAEPSPD-------LCVEEKEVTSALKDLGISSTHLQNNTCTDLRSPITGTYRILLHSIQKRRSVEMVGYSALYPE---DFRDSKHWDSK 430
BLAST of Serine/threonine-protein kinase NIM1 vs. Ensembl Cavefish
Match: ENSAMXT00000046776.1 (serine/threonine-protein kinase NIM1-like [Source:NCBI gene;Acc:103034090]) HSP 1 Score: 335.109 bits (858), Expect = 3.720e-109 Identity = 170/411 (41.36%), Postives = 249/411 (60.58%), Query Frame = 2
Query: 161 TSFDKYTEEMNCNENVKKEIMLGKRVGLYRIKGNLGNGNFSQVKLAVHLLTREEVAIKILDKTKLDAKTQKLLSREISSMERLHHPNIIRLYEVIEDIRKMHLVMEYASGGELHSLIVSHGKIPESISKLIFAQIIAAINHLHSQNVIHRDLKAENIFFAGNKSETNQDNSKLNSTTKKSDFTDNAFSRLVHVKLGDFGFSTLAQPAEKLTTFCGSPPYAAPELFQADWYYGTCVDIWALGILLYFMLCGCMPFRGESVGLLKKLILAGNYVCPNWLPVDAKNLIQQILKQNPDQRLTIQQIKNHQWLSGQHFPDAYQPFKATPLMPE-IETISHAXXXXXXXXXXXXPLEKDSIEASKMLLRLGITDKHIEVAQAQTVRNYVTGTYRIMLHRVHRNRRMSNIPHSLSNPE 1390
T+F++ ++ +E V ++ G+R+GLY ++G +G+GNFSQV+L +H +T+E VA+KILDK +LD +QKL + EIS+ME+L HPNI+RLYEV+E R+MHLVMEYA+GGEL+S I S G++ SK++F+QI++A+ H+H N+IHRDLKAEN+++ N FT VK+GDFGFST QP++ L TFCGSPPYAAPELF+ Y G VD+WALG+LL+FM+ PF G S+ LK IL G Y P+++P +N+I+ +L+Q P R ++ ++ WL + QP+ P PE + S L D +L LGIT+ H++ ++ +TG YRI+LHRV R + + ++ P+
Sbjct: 64 TAFEQAVHDLTNSERVINDLTFGRRIGLYELRGEIGSGNFSQVRLGIHDITKERVAVKILDKVRLDRHSQKLFASEISNMEKLSHPNIVRLYEVVETFRRMHLVMEYAAGGELYSRISSRGRLSGVESKVVFSQILSAVRHMHDNNIIHRDLKAENVYYTAN-------------------FT---------VKVGDFGFSTECQPSQILKTFCGSPPYAAPELFKDKGYIGAFVDLWALGVLLFFMVTASFPFTGTSLSKLKTCILQGAYAIPSYVPDPCQNIIRGLLRQVPADRSSLAKVMASIWLKDVEY---LQPYPPAPSTPEHLADFSRV-------------LSPDDKVVKVVLEELGITEIHLKNNAHLDCQSPLTGVYRIVLHRVQRRSTVEAVGYTSLCPK 430
BLAST of Serine/threonine-protein kinase NIM1 vs. Ensembl Cavefish
Match: si:ch211-22d5.2 (serine/threonine-protein kinase NIM1-like [Source:NCBI gene;Acc:103038601]) HSP 1 Score: 317.775 bits (813), Expect = 6.745e-103 Identity = 163/415 (39.28%), Postives = 253/415 (60.96%), Query Frame = 2
Query: 110 SLDILPKISETEENNVE-TSFDKYTEEMNCNENVKKEIMLGKRVGLYRIKGNLGNGNFSQVKLAVHLLTREEVAIKILDKTKLDAKTQKLLSREISSMERLHHPNIIRLYEVIEDIRKMHLVMEYASGGELHSLIVSHGKIPESISKLIFAQIIAAINHLHSQNVIHRDLKAENIFFAGNKSETNQDNSKLNSTTKKSDFTDNAFSRLVHVKLGDFGFST-LAQPAEKLTTFCGSPPYAAPELFQADWYYGTCVDIWALGILLYFMLCGCMPFRGESVGLLKKLILAGNYVCPNWLPVDAKNLIQQILKQNPDQRLTIQQIKNHQWLSGQHFPDAYQPFKATPLMPEIETISHAXXXXXXXXXXXXPLEKDSIEASKMLLRLGITDKHIEVAQAQTVRNYVTGTYRIMLHRVHRN 1348
S D P+ E E++ T +K T +M +E K++ +GKRVG Y+++G +G GNFS+VKLA H LT+++VAIK+++K +LD +TQ++LSREIS+ME L+HPN++RLYEV+E +++LV+EYA GG+LHS + S GK+ + SK++FAQI++A+ ++H ++IHRD+KAEN+ ++ + VK+ DFGFST + + L TFCGSPPYAAPELF+ + Y G VD+WA+G+LL+FM+ G MPFR ++V L++ ++ G Y P W+ + LI+ ILK +P +R + Q+ +WL +P + + +E + E++ + A+ L LG+T +HI Q + R+ TG YRI+LHR+ RN
Sbjct: 20 SSDCGPEKEEEEDSPKRLTPLEKLTRDMCHDEKTIKDLTVGKRVGFYKVRGEIGCGNFSKVKLAFHALTKDKVAIKVMEKMRLDLQTQRMLSREISNMESLYHPNVLRLYEVVETPCRLYLVLEYAGGGDLHSRLTSEGKLTDPESKIVFAQILSAVKYMHDNSIIHRDMKAENVLYSCSGC----------------------------VKVADFGFSTRINNRNQMLDTFCGSPPYAAPELFKDESYLGPPVDVWAMGVLLFFMVTGTMPFRADTVPKLRRSVIEGVYALPMWVSAPCQRLIRGILKPDPSERYALDQMLGCEWLLPVELFRTARPLQQLNPVYLLEAGTGRMMQ-----------EEEDVRAA--LQELGVTQEHILDNQCKNSRSPFTGMYRIVLHRIQRN 393
BLAST of Serine/threonine-protein kinase NIM1 vs. Ensembl Cavefish
Match: mark3b (MAP/microtubule affinity-regulating kinase 3 [Source:NCBI gene;Acc:103043586]) HSP 1 Score: 256.144 bits (653), Expect = 9.643e-76 Identity = 122/305 (40.00%), Postives = 192/305 (62.95%), Query Frame = 2
Query: 236 VGLYRIKGNLGNGNFSQVKLAVHLLTREEVAIKILDKTKLDAKTQKLLSREISSMERLHHPNIIRLYEVIEDIRKMHLVMEYASGGELHSLIVSHGKIPESISKLIFAQIIAAINHLHSQNVIHRDLKAENIFFAGNKSETNQDNSKLNSTTKKSDFTDNAFSRLVHVKLGDFGFSTLAQPAEKLTTFCGSPPYAAPELFQADWYYGTCVDIWALGILLYFMLCGCMPFRGESVGLLKKLILAGNYVCPNWLPVDAKNLIQQILKQNPDQRLTIQQIKNHQWLSGQHFPDAYQPFKATPLMPEIE 1150
+G YR+ +G GNF++VKLA H+LT EVAIKI+DKT+L+ + + L RE+ M+ L+HPNI++L+EVIE + ++LVMEYASGGE+ +V+HG++ E ++ F QI++A+ + H ++++HRDLKAEN+ + +++K+ DFGFS KL TFCGSPPYAAPELFQ Y G VD+W+LG++LY ++ G +PF G+++ L++ +L G Y P ++ D +NL+++ L NP +R T++QI +W++ D +PF + PE++
Sbjct: 65 IGNYRLLKTIGKGNFAKVKLARHILTGREVAIKIIDKTQLNPTSLQKLFREVRIMKILNHPNIVKLFEVIETEKTLYLVMEYASGGEVFDYLVAHGRMKEKEARAKFRQIVSAVQYCHQKHIVHRDLKAENLLLDAD----------------------------MNIKIADFGFSNEFTLGNKLDTFCGSPPYAAPELFQGKKYDGPEVDVWSLGVILYTLVSGSLPFDGQNLKELRERVLRGKYRIPFYMSTDCENLLKRFLVLNPSKRGTLEQIMKDRWINAGFEEDELKPF----VEPELD 337
BLAST of Serine/threonine-protein kinase NIM1 vs. Ensembl Sea Lamprey
Match: NIM1K (NIM1 serine/threonine protein kinase [Source:HGNC Symbol;Acc:HGNC:28646]) HSP 1 Score: 341.273 bits (874), Expect = 3.057e-113 Identity = 182/400 (45.50%), Postives = 242/400 (60.50%), Query Frame = 2
Query: 161 TSFDKYTEEMNCNENVKKEIMLGKRVGLYRIKGNLGNGNFSQVKLAVHLLTREEVAIKILDKTKLDAKTQKLLSREISSMERLHHPNIIRLYEVIEDIRKMHLVMEYASGGELHSLIVSHGKIPESISKLIFAQIIAAINHLHSQNVIHRDLKAENIFFAGNKSETNQDNSKLNSTTKKSDFTDNAFSRLVHVKLGDFGFSTLAQPAEKLTTFCGSPPYAAPELFQADWYYGTCVDIWALGILLYFMLCGCMPFRGESVGLLKKLILAGNYVCPNWLPVDAKNLIQQILKQNPDQRLTIQQIKNHQWLSGQHFPDAYQPFKATP-LMPEIETISHAXXXXXXXXXXXXPLEKDSIEASKMLLRLGITDKHIE-VAQAQTVRNYVTGTYRIMLHRVHRNRR 1354
T F+K T E +E +E +LGKR+ YRI+G +G+GNFSQVKL VHLLTR++VAIK+LDK+++D +T LLS EI +ME L HPN++RLYEV++ ++HLVMEYA GGEL + + + G +P +KL+FAQI AA+ H+H V+HRDLKAEN+F+AGN VK+GDFGFST P E+L+TFCGSPPYAAPELF+ Y G VD+WALG+LL+F+ G +PFR ++VG LK IL G + P +P NLI IL+ PD+R T QI +WL G H P+ Y P+ P +P ++ +E ++L LGIT + R+ VTGTYRI+LHRV R RR
Sbjct: 15 TPFEKLTWEARTDEVAVRESVLGKRISFYRIRGEIGSGNFSQVKLGVHLLTRDKVAIKLLDKSRMDERTLCLLSCEILAMELLRHPNVVRLYEVVDTPSRLHLVMEYAPGGELFTRVSTVGALPHDEAKLVFAQITAAVKHMHECGVVHRDLKAENVFYAGN----------------------------CWVKVGDFGFSTTVTPNERLSTFCGSPPYAAPELFRDQSYLGPQVDVWALGVLLFFIASGTLPFRADTVGKLKLSILDGCFSVPLRVPASCCNLITTILRPLPDERPTAAQILRSEWLRGIHAPEPYPPYPLGPRYLPSCSLLAEE-----------------LLEVRELLEELGITSDVVRNNGDDGDARSCVTGTYRIVLHRVQRRRR 369
BLAST of Serine/threonine-protein kinase NIM1 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000006410.1 (pep scaffold:Pmarinus_7.0:GL476370:17405:31068:-1 gene:ENSPMAG00000005688.1 transcript:ENSPMAT00000006410.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 258.84 bits (660), Expect = 2.511e-78 Identity = 122/296 (41.22%), Postives = 189/296 (63.85%), Query Frame = 2
Query: 236 VGLYRIKGNLGNGNFSQVKLAVHLLTREEVAIKILDKTKLDAKTQKLLSREISSMERLHHPNIIRLYEVIEDIRKMHLVMEYASGGELHSLIVSHGKIPESISKLIFAQIIAAINHLHSQNVIHRDLKAENIFFAGNKSETNQDNSKLNSTTKKSDFTDNAFSRLVHVKLGDFGFSTLAQPAEKLTTFCGSPPYAAPELFQADWYYGTCVDIWALGILLYFMLCGCMPFRGESVGLLKKLILAGNYVCPNWLPVDAKNLIQQILKQNPDQRLTIQQIKNHQWLSGQHFPDAYQPFK 1123
+G YR+ +G GNF++VKLA H+LT EVAIKI+DKT+L+ + + L RE+ M+ L+HPNI++LYEVIE + ++LVMEYASGGE+ +V+HG++ E ++ F QI++A+ + H +N++HRDLKAEN+ + +H+K+ DFGFS KL TFCGSPPYAAPELFQ Y G VD+W+LG++LY ++ G +PF G+++ L++ +L G Y P ++ D +NL+++ L NP +R +++QI +W++ H D +P++
Sbjct: 36 IGNYRLLKTIGKGNFAKVKLARHILTGREVAIKIIDKTQLNPTSLQKLFREVRIMKCLNHPNIVKLYEVIETEKTLYLVMEYASGGEVFDYLVAHGRMKEKEARAKFRQIVSAVQYCHQKNIVHRDLKAENLLLDVD----------------------------MHIKIADFGFSNEFTLVNKLDTFCGSPPYAAPELFQGKKYDGPEVDVWSLGVILYTLVSGSLPFDGQNLKELREKVLRGKYRIPFYMSTDCENLLKRFLVLNPIKRGSLEQIMRDRWMNVGHEEDELKPYQ 303
BLAST of Serine/threonine-protein kinase NIM1 vs. Ensembl Sea Lamprey
Match: MARK3 (microtubule affinity regulating kinase 3 [Source:HGNC Symbol;Acc:HGNC:6897]) HSP 1 Score: 251.906 bits (642), Expect = 4.165e-78 Identity = 120/310 (38.71%), Postives = 195/310 (62.90%), Query Frame = 2
Query: 191 NCNENVKKEIMLGKRVGLYRIKGNLGNGNFSQVKLAVHLLTREEVAIKILDKTKLDAKTQKLLSREISSMERLHHPNIIRLYEVIEDIRKMHLVMEYASGGELHSLIVSHGKIPESISKLIFAQIIAAINHLHSQNVIHRDLKAENIFFAGNKSETNQDNSKLNSTTKKSDFTDNAFSRLVHVKLGDFGFSTLAQPAEKLTTFCGSPPYAAPELFQADWYYGTCVDIWALGILLYFMLCGCMPFRGESVGLLKKLILAGNYVCPNWLPVDAKNLIQQILKQNPDQRLTIQQIKNHQWLSGQHFPDAYQPF 1120
N E+ + +RVG YR+ +G GNF++VKLA H LT +EVA+KI+DKT+L+ + + L RE+ M+ L+HPNI++L+EVIE + ++LVMEY+SGGE+ +V+HG++ E ++ F QI++A+ + H ++++HRDLKAEN+ + +++K+ DFGFS KL TFCGSPPYAAPELFQ Y G VD+W+LG++LY ++ G +PF G+++ L++ +L G Y P ++ D +NL+++ L +P +R +++QI +W++ +H D +P+
Sbjct: 50 NSAEDAQPPSGQTQRVGNYRLLKTIGKGNFAKVKLAKHTLTGQEVAVKIIDKTQLNTSSLQKLFREVRIMKVLNHPNIVKLFEVIEMEKTLYLVMEYSSGGEVFDYLVAHGRMKEKEARAKFRQIVSAVQYCHQKHIVHRDLKAENLLLDAD----------------------------MNIKIADFGFSNEFTLGSKLDTFCGSPPYAAPELFQGKKYDGPEVDVWSLGVILYTLVSGSLPFDGQNLKELRERVLRGKYRIPFYMSTDCENLLKRFLVLHPLKRGSLEQIMKDRWMNVKHEDDELKPY 331
BLAST of Serine/threonine-protein kinase NIM1 vs. Ensembl Sea Lamprey
Match: MARK3 (microtubule affinity regulating kinase 3 [Source:HGNC Symbol;Acc:HGNC:6897]) HSP 1 Score: 257.299 bits (656), Expect = 1.195e-76 Identity = 125/335 (37.31%), Postives = 201/335 (60.00%), Query Frame = 2
Query: 122 LPKISETEENNVETSFDKYTEE--MNCNENVKKEIMLGKRVGLYRIKGNLGNGNFSQVKLAVHLLTREEVAIKILDKTKLDAKTQKLLSREISSMERLHHPNIIRLYEVIEDIRKMHLVMEYASGGELHSLIVSHGKIPESISKLIFAQIIAAINHLHSQNVIHRDLKAENIFFAGNKSETNQDNSKLNSTTKKSDFTDNAFSRLVHVKLGDFGFSTLAQPAEKLTTFCGSPPYAAPELFQADWYYGTCVDIWALGILLYFMLCGCMPFRGESVGLLKKLILAGNYVCPNWLPVDAKNLIQQILKQNPDQRLTIQQIKNHQWLSGQHFPDAYQPF 1120
LP ++E + N T K + C ++ + +G YR+ +G GNF++VKLA H LT EVA+KI+DKT+L+ + + L RE+ M+ L+HPNI++L+EVIE + ++LVMEYASGGE+ +V+HG++ E ++ F QI++A+ + H ++++HRDLKAEN+ + +++K+ DFGFS KL TFCGSPPYAAPELFQ Y G VD+W+LG++LY ++ G +PF G+++ L++ +L G Y P ++ D +NL+++ L NP +R +++QI +W++ H D +P+
Sbjct: 11 LPTVNERDTENDTTVVTKPSRSSGARCRNSITVAEVEHAHIGTYRLLKTIGKGNFAKVKLARHTLTGREVAVKIIDKTQLNPSSLQKLFREVRIMKILNHPNIVKLFEVIETEKTLYLVMEYASGGEVFDYLVAHGRMKEKEARAKFRQIVSAVQYCHQKHIVHRDLKAENLLLDAD----------------------------MNIKIADFGFSNEFTLGSKLDTFCGSPPYAAPELFQGKKYDGPEVDVWSLGVILYTLVSGSLPFDGQNLKELRERVLRGKYRIPFYMSTDCENLLKRFLVLNPAKRGSLEQIMKDRWMNVGHEEDELKPY 317
BLAST of Serine/threonine-protein kinase NIM1 vs. Ensembl Sea Lamprey
Match: snrka (SNF related kinase a [Source:ZFIN;Acc:ZDB-GENE-040718-84]) HSP 1 Score: 227.639 bits (579), Expect = 3.977e-67 Identity = 130/377 (34.48%), Postives = 209/377 (55.44%), Query Frame = 2
Query: 227 GKRVGLYRIKGNLGNGNFSQVKLAVHLLTREEVAIKILDKTKLDA-KTQKLLSREISSMERLHHPNIIRLYEVIEDIRKMHLVMEYASGGELHSLIVSH-GKIPESISKLIFAQIIAAINHLHSQNVIHRDLKAENIFFAGNKSETNQDNSKLNSTTKKSDFTDNAFSRLVHVKLGDFGFSTLAQPAEKLTTFCGSPPYAAPELFQADWYYGTCVDIWALGILLYFMLCGCMPFRGESVGLLKKLILAGNYVCPNWLPVDAKNLIQQILKQNPDQRLTIQQIKNHQWLSGQHFPDAYQPFKATPLMPEIETISHAXXXXXXXXXXXXPLEKDSIEASKMLLRLGITDKHIEVAQAQTVRNYVTGTYRIMLHRVHRNR 1351
GK GLY ++ LG G+F+ VKLA H+ T E+VA+K++DK+KLD T L +E+ M+ + HPN++RLYEVI+ K++L++E A GG+L+ I+ H G + E ++K FAQ++ AI++ H +V+HRDLK EN+ F F + VKL DFGFS L QP KL T CGS Y+APE+ D Y VDIW+LG++LY ++CG PF+ + +I+ Y P + + K+LI ++L+++P +R ++Q+++ H WL G A + +TPL+ +++S E + I +L + D +E +A N++T TY ++ R+ +++
Sbjct: 10 GKIAGLYDLEKTLGRGHFAVVKLARHVFTGEKVAVKVIDKSKLDPMATSHLFQQEVRCMKLVQHPNVVRLYEVIDTQTKLYLILELADGGDLYDYIMRHEGGLQEELAKTYFAQVVHAISYCHRLHVVHRDLKPENVVF---------------------------FEKQGLVKLTDFGFSNLFQPGTKLATSCGSLAYSAPEILLGDEYDAPAVDIWSLGVILYMLVCGHPPFQEANDSETLTMIMDCKYTVPAHISRECKDLIARMLQRDPRRRASLQEMEGHPWLCGVDPSPAAK--CSTPLI-SYKSLSED--------------EHNGIVQRMVLGSIAERDAIVEALEADRY-NHITATYFLLAERILKDK 341
BLAST of Serine/threonine-protein kinase NIM1 vs. Ensembl Yeast
Match: SNF1 (AMP-activated S/T protein kinase; forms a complex with Snf4p and members of the Sip1p/Sip2p/Gal83p family; required for transcription of glucose-repressed genes, thermotolerance, sporulation, and peroxisome biogenesis; regulates nucleocytoplasmic shuttling of Hxk2p; regulates filamentous growth and acts as a non-canonical GEF, activating Arf3p during invasive growth; SUMOylation by Mms21p inhibits its function and targets Snf1p for destruction via the Slx5-Slx8 Ub ligase [Source:SGD;Acc:S000002885]) HSP 1 Score: 194.512 bits (493), Expect = 6.830e-55 Identity = 111/295 (37.63%), Postives = 159/295 (53.90%), Query Frame = 2
Query: 203 NVKKEIMLGKRVGLYRIKGNLGNGNFSQVKLAVHLLTREEVAIKILDKTKL-DAKTQKLLSREISSMERLHHPNIIRLYEVIEDIRKMHLVMEYASGGELHSLIVSHGKIPESISKLIFAQIIAAINHLHSQNVIHRDLKAENIFFAGNKSETNQDNSKLNSTTKKSDFTDNAFSRLVHVKLGDFGFSTLAQPAEKLTTFCGSPPYAAPELFQADWYYGTCVDIWALGILLYFMLCGCMPFRGESVGLLKKLILAGNYVCPNWLPVDAKNLIQQILKQNPDQRLTIQQIKNHQWL 1084
N K + G +G Y+I LG G+F +VKLA H T ++VA+KI++K L + Q + REIS + L HP+II+LY+VI+ ++ +V+EYA G EL IV K+ E ++ F QII+A+ + H ++HRDLK EN+ + LN VK+ DFG S + L T CGSP YAAPE+ Y G VD+W+ G++LY MLC +PF ES+ +L K I G Y P +L A LI+++L NP R++I +I W
Sbjct: 41 NPKSSLADGAHIGNYQIVKTLGEGSFGKVKLAYHTTTGQKVALKIINKKVLAKSDMQGRIEREISYLRLLRHPHIIKLYDVIKSKDEIIMVIEYA-GNELFDYIVQRDKMSEQEARRFFQQIISAVEYCHRHKIVHRDLKPENLLL----------DEHLN------------------VKIADFGLSNIMTDGNFLKTSCGSPNYAAPEVISGKLYAGPEVDVWSCGVILYVMLCRRLPFDDESIPVLFKNISNGVYTLPKFLSPGAAGLIKRMLIVNPLNRISIHEIMQDDWF 306
BLAST of Serine/threonine-protein kinase NIM1 vs. Ensembl Yeast
Match: KCC4 (Protein kinase of the bud neck involved in the septin checkpoint; associates with septin proteins, negatively regulates Swe1p by phosphorylation, shows structural homology to bud neck kinases Gin4p and Hsl1p; KCC4 has a paralog, GIN4, that arose from the whole genome duplication [Source:SGD;Acc:S000000529]) HSP 1 Score: 166.007 bits (419), Expect = 5.597e-44 Identity = 98/297 (33.00%), Postives = 155/297 (52.19%), Query Frame = 2
Query: 236 VGLYRIKGNLGNGNFSQVKLAVHLLTREEVAIKILDKTKL---------DAKTQKLLSREISSMERLHHPNIIRLYEVIEDIRKMHLVMEYASGGELHSLIVSHGKIPESISKLIFAQIIAAINHLHSQNVIHRDLKAENIFFAGNKSETNQDNSKLNSTTKKSDFTDNAFSRLVHVKLGDFGFSTLAQPAEKLTTFCGSPPYAAPELFQADWYYGTCVDIWALGILLYFMLCGCMPFRGESVGLLKKLIL---AGNYVCPNWLPV--DAKNLIQQILKQNPDQRLTIQQIKNHQWL 1084
+G +++ LG G+ +V+LA H T A+K++ K+ D+ + REI M+ L HPN++ LY+V E ++L++EYA GEL +L+V HG +PE + F QII I++ H+ ++HRDLK EN+ ++K+ DFG + L A+ L T CGSP YAAPE+ Y G D+W+ G++L+ +L G +PF E+ G ++ L+L G + PN + DA++LI +IL +P QR+ I+ I +H L
Sbjct: 18 IGPWKLGETLGFGSTGKVQLAQHERTGHRTAVKVISKSIFNNNGNHSNDDSVLPYNIEREIVIMKLLSHPNVLSLYDVWETNNNLYLILEYAEKGELFNLLVDHGPLPEREAINCFRQIIIGISYCHALGIVHRDLKPENLLLDS----------------------------FYNIKIADFGMAALQTDADLLETSCGSPHYAAPEIVSGLPYEGFASDVWSCGVILFALLTGRLPFDEEN-GNVRDLLLKVQKGQFEMPNDTEISRDAQDLIGKILVVDPRQRIKIRDILSHPLL 285
BLAST of Serine/threonine-protein kinase NIM1 vs. Ensembl Yeast
Match: KIN1 (Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; KIN1 has a paralog, KIN2, that arose from the whole genome duplication [Source:SGD;Acc:S000002529]) HSP 1 Score: 164.081 bits (414), Expect = 2.658e-43 Identity = 105/335 (31.34%), Postives = 170/335 (50.75%), Query Frame = 2
Query: 230 KRVGLYRIKGNLGNGNFSQVKLAVHLLTREEVAIKILD----------------KTKLDA-KTQKLLSREISSMER----------LHHPNIIRLYEVIEDIRKMHLVMEYASGGELHSLIVSHGKIPESISKLIFAQIIAAINHLHSQNVIHRDLKAENIFFAGNKSETNQDNSKLNSTTKKSDFTDNAFSRLVHVKLGDFGFSTLAQPAEKLTTFCGSPPYAAPELFQADWYYGTCVDIWALGILLYFMLCGCMPFRGESVGLLKKLILAGNYVCPNWLPVDAKNLIQQILKQNPDQRLTIQQIKNHQWL--SGQHFPDAYQPFKATPLMPEI 1147
K +G + +G G+ +VKLA H T E A+KI++ K + D + QK L +EIS +R L+HP+I RL+E+ +++ EY SGG+L I+ HG I E ++ I +A+ +LH+ N++HRDLK ENI + D+S+ +K+ DFG S + ++L TFCGS +AAPEL +A+ Y G VD+W+ G++L+ ++CG +PF E+ +L + I G P L ++ +L+ ++L +P +R T++Q+ H W+ P +Y P K PL E+
Sbjct: 115 KSLGDWEFVETVGAGSMGKVKLAKHRYTNEVCAVKIVNRATKAFLHKEQMLPPPKNEQDVLERQKKLEKEISRDKRTIREASLGQILYHPHICRLFEMCTLSNHFYMLFEYVSGGQLLDYIIQHGSIREHQARKFARGIASALIYLHANNIVHRDLKIENIMIS--------DSSE--------------------IKIIDFGLSNIYDSRKQLHTFCGSLYFAAPELLKANPYTGPEVDVWSFGVVLFVLVCGKVPFDDENSSVLHEKIKQGKVEYPQHLSIEVISLLSKMLVVDPKRRATLKQVVEHHWMVRGFNGPPPSYLP-KRVPLTIEM 420
BLAST of Serine/threonine-protein kinase NIM1 vs. Ensembl Yeast
Match: HSL1 (Nim1p-related protein kinase; septin-binding kinase that localizes to the bud neck septin ring and regulates the morphogenesis checkpoint; phosphorylates Hsl7p and cooperates with Elm1p to recruit Hsl7p to the mother-bud neck, as a prerequisite for the subsequent recruitment, phosphorylation, and degradation of Swe1p; autophosphorylation enhances interactions with Hsl7p [Source:SGD;Acc:S000001584]) HSP 1 Score: 162.925 bits (411), Expect = 7.772e-43 Identity = 97/316 (30.70%), Postives = 161/316 (50.95%), Query Frame = 2
Query: 236 VGLYRIKGNLGNGNFSQVKLAVHLLTREEVAIKILDKTKL---------------------DAKTQKLLSREISS---------------MERLHHPNIIRLYEVIEDIRKMHLVMEYASGGELHSLIVSHGKIPESISKLIFAQIIAAINHLHSQNVIHRDLKAENIFFAGNKSETNQDNSKLNSTTKKSDFTDNAFSRLVHVKLGDFGFSTLAQPAEKLTTFCGSPPYAAPELFQADWYYGTCVDIWALGILLYFMLCGCMPFRGESVGLLKKLILAGNYVCPNWLPVDAKNLIQQILKQNPDQRLTIQQIKNH 1075
VG +++ LG G+ +V+LA ++ T + AIKI+ K K + + + SRE S+ M+ + H N++ L+EV E+ +++LV+EY GGEL +VS GK+PE + F QI+ +++ HS N+ HRDLK EN+ ++ N + +K+ DFG + L P + L T CGSP YA+PE+ Y+G D+W+ GI+L+ +L G +PF +++ L + +G Y P+ L +A++LI +IL +P++R+T Q+I H
Sbjct: 78 VGPWKLGKTLGKGSSGRVRLAKNMETGQLAAIKIVPKKKAFVHCSNNGTVPNSYSSSMVTSNVSSPSIASREHSNHSQTNPYGIEREIVIMKLISHTNVMALFEVWENKSELYLVLEYVDGGELFDYLVSKGKLPEREAIHYFKQIVEGVSYCHSFNICHRDLKPENLLL-------DKKNRR--------------------IKIADFGMAALELPNKLLKTSCGSPHYASPEIVMGRPYHGGPSDVWSCGIVLFALLTGHLPFNDDNIKKLLLKVQSGKYQMPSNLSSEARDLISKILVIDPEKRITTQEILKH 366
BLAST of Serine/threonine-protein kinase NIM1 vs. Ensembl Yeast
Match: KIN4 (Serine/threonine protein kinase; inhibits the mitotic exit network (MEN) when the spindle position checkpoint is activated; localized asymmetrically to mother cell cortex, spindle pole body and bud neck; KIN4 has a paralog, FRK1, that arose from the whole genome duplication [Source:SGD;Acc:S000005759]) HSP 1 Score: 157.532 bits (397), Expect = 2.295e-41 Identity = 104/333 (31.23%), Postives = 170/333 (51.05%), Query Frame = 2
Query: 152 NVETSFDKYTEEMN------CNENVKKEIMLGKRVGLYRIKGNLGNGNFSQVKLAVHLLTR-----EEVAIKIL--DKTKLDAKTQKLLSREISSMERLHHPNIIRLYEVIEDIRKMHLVMEYASGGELHSLIVSHGKIPESISKLIFAQIIAAINHLHSQNVIHRDLKAENIFFAGNKSETNQDNSKLNSTTKKSDFTDNAFSRLVHVKLGDFGF-STLAQPAEKLTTFCGSPPYAAPEL-FQADWYYGTCVDIWALGILLYFMLCGCMPF-------RGESVGLLKKLILAGNYVCPNWLPVDAKNLIQQILKQNPDQRLTIQQIKNHQWL 1084
NV T D+++ + N ++N +K G Y I LG G F +VKL + ++VAIK++ D K DA + + REI++++ L HPNII L EV+++ + + +V+E+ SGGE + I ++ ES + +FAQ+I+ +N++H + ++HRDLK EN+ +K E ++ + DFGF + + E + T CGSP YAAPEL Y D+W+ G++LY ML G +P+ G+ + L K I P ++ ++L+++IL NP +R+ +Q IK H WL
Sbjct: 13 NVVTDRDRHSLQRNNEILHPIHKNQRKHATFGP----YIIGSTLGEGEFGKVKLGWTKASSSNEVPKQVAIKLIRRDTIKKDADKEIKIYREINALKHLTHPNIIYLEEVLQNSKYIGIVLEFVSGGEFYKYIQRKRRLKESSACRLFAQLISGVNYMHYKGLVHRDLKLENLLL--DKHE--------------------------NLVITDFGFVNEFFEDNELMKTSCGSPCYAAPELVVSTKAYEARKADVWSCGVILYAMLAGYLPWDDDHENPTGDDIARLYKYITQTPLKFPEYITPIPRDLLRRILVPNPRRRINLQTIKRHVWL 313
BLAST of Serine/threonine-protein kinase NIM1 vs. Ensembl Nematostella
Match: EDO46937 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RNJ8]) HSP 1 Score: 369.777 bits (948), Expect = 1.010e-123 Identity = 188/397 (47.36%), Postives = 249/397 (62.72%), Query Frame = 2
Query: 161 TSFDKYTEEMNCNENVKKEIMLGKRVGLYRIKGNLGNGNFSQVKLAVHLLTREEVAIKILDKTKLDAKTQKLLSREISSMERLHHPNIIRLYEVIEDIRKMHLVMEYASGGELHSLIVSHGKIPESISKLIFAQIIAAINHLHSQNVIHRDLKAENIFFAGNKSETNQDNSKLNSTTKKSDFTDNAFSRLVHVKLGDFGFSTLAQPAEKLTTFCGSPPYAAPELFQADWYYGTCVDIWALGILLYFMLCGCMPFRGESVGLLKKLILAGNYVCPNWLPVDAKNLIQQILKQNPDQRLTIQQIKNHQWLSGQHFPDAYQPFKATPLMPEIETISHAXXXXXXXXXXXXPLEKDSIEASKMLLRLGITDKHIEVAQAQTVRNYVTGTYRIMLHRVHRNR 1351
T + + +++ E KEI LGKR+G YRI+G LG GNF QVK+ + LTRE+VAIKILDKTKLD KTQ+LLSREISSME LHHPNIIRLYEVIE + K+H+VMEYA GGEL + I + GK+PE I+K ++ Q+++A+ H+H ++IHRDLKAEN+F AG VK+GDFGFST A L TFCGSPPYAAPELF+ + Y G VDIWA+GI+LYFM+ G MPFR E+VG LKK IL G++ P++L + LI+ IL+ P R +I+ IK WL + P A + + TP + + S +D EA +L LGIT +H+ ++ R+ +TGTYRI+LHR+ + +
Sbjct: 25 TPYQRLNHDLSNEERYLKEISLGKRIGFYRIRGELGTGNFCQVKMGIQSLTREKVAIKILDKTKLDQKTQRLLSREISSMENLHHPNIIRLYEVIETLSKLHIVMEYACGGELFAKISNEGKLPERIAKKLYGQVLSAVEHMHDNDIIHRDLKAENVFIAGPNL----------------------------VKVGDFGFSTTATKESALNTFCGSPPYAAPELFKDEHYIGIYVDIWAMGIMLYFMVTGVMPFRAETVGKLKKCILDGSFFLPDFLSDGCRQLIRNILRPVPTDRFSIEDIKRSTWLETETLPQADERYSLTPPILAKQAAS-----------------EDEKEALVVLNDLGITTEHM--SRCHDSRDSITGTYRIVLHRIQKRK 374
BLAST of Serine/threonine-protein kinase NIM1 vs. Ensembl Nematostella
Match: EDO46035 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RR22]) HSP 1 Score: 249.21 bits (635), Expect = 3.754e-78 Identity = 116/285 (40.70%), Postives = 184/285 (64.56%), Query Frame = 2
Query: 233 RVGLYRIKGNLGNGNFSQVKLAVHLLTREEVAIKILDKTKLDAKTQKLLSREISSMERLHHPNIIRLYEVIEDIRKMHLVMEYASGGELHSLIVSHGKIPESISKLIFAQIIAAINHLHSQNVIHRDLKAENIFFAGNKSETNQDNSKLNSTTKKSDFTDNAFSRLVHVKLGDFGFSTLAQPAEKLTTFCGSPPYAAPELFQADWYYGTCVDIWALGILLYFMLCGCMPFRGESVGLLKKLILAGNYVCPNWLPVDAKNLIQQILKQNPDQRLTIQQIKNHQWLS 1087
R+G Y I+ +G GNF+ VKLA H +T+ +VA+KI+DK++LD + RE+ M++L HP+II+L+EV+E R ++LV EYAS GE+ +V+HG++ E +K F QI++AI + H N++HRDLKAEN+ ++D +++K+ DFGFS + Q +KL T+CGSPPYAAPELF+ Y G VDIW+LG++LY ++CG +PF G ++ L+ +L G + P ++ + ++LI+ +L ++P +R TI QI+ H+W++
Sbjct: 16 RIGFYNIEKTIGKGNFAVVKLATHCITKSKVAVKIIDKSQLDDDNLTKVKREVKVMKKLAHPHIIKLHEVMETERMLYLVTEYASKGEIFDYLVAHGRMQEKEAKNTFNQIVSAIEYCHKMNIVHRDLKAENLLL-------DED---------------------MNIKIADFGFSNIFQADKKLKTWCGSPPYAAPELFEGKEYLGPEVDIWSLGVVLYVLVCGALPFDGSTLQSLRSRVLDGRFRIPFFMSTECEHLIRHMLVRDPVKRFTIPQIRQHKWMA 272
BLAST of Serine/threonine-protein kinase NIM1 vs. Ensembl Nematostella
Match: EDO30768 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SZV3]) HSP 1 Score: 252.677 bits (644), Expect = 6.520e-76 Identity = 119/295 (40.34%), Postives = 186/295 (63.05%), Query Frame = 2
Query: 236 VGLYRIKGNLGNGNFSQVKLAVHLLTREEVAIKILDKTKLDAKTQKLLSREISSMERLHHPNIIRLYEVIEDIRKMHLVMEYASGGELHSLIVSHGKIPESISKLIFAQIIAAINHLHSQNVIHRDLKAENIFFAGNKSETNQDNSKLNSTTKKSDFTDNAFSRLVHVKLGDFGFSTLAQPAEKLTTFCGSPPYAAPELFQADWYYGTCVDIWALGILLYFMLCGCMPFRGESVGLLKKLILAGNYVCPNWLPVDAKNLIQQILKQNPDQRLTIQQIKNHQWLSGQHFPDAYQPF 1120
+G YR+ +G GNF++VKLA H+ T +EVAIKI+DKT+L+ + + L RE+ M+ L HPNI++LYEVIE + ++LVMEYASGGE+ +V+HG++ E ++ F QI++A+ + H ++VIHRDLKAEN+ + +++K+ DFGFS P KL TFCGSPPYAAPELFQ Y G VD+W+LG++LY ++ G +PF G+++ L++ +L G Y P ++ D +NL+++ L NP +R ++Q +W++ + + +P+
Sbjct: 35 IGRYRLIKTIGKGNFAKVKLAKHVPTGKEVAIKIIDKTQLNPSSLQKLFREVRIMKFLDHPNIVKLYEVIETDKTLYLVMEYASGGEVFDYLVAHGRMKEKEARAKFRQIVSAVQYCHQKHVIHRDLKAENLLLDAD----------------------------MNIKIADFGFSNEFTPGNKLDTFCGSPPYAAPELFQGKKYDGPEVDVWSLGVILYTLVSGSLPFDGQNLKELRERVLRGKYRIPFYMSTDCENLLKKFLVLNPQKRARLEQTMTDKWMNINYELNELKPY 301
BLAST of Serine/threonine-protein kinase NIM1 vs. Ensembl Nematostella
Match: EDO31932 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SWI1]) HSP 1 Score: 232.261 bits (591), Expect = 5.614e-71 Identity = 130/376 (34.57%), Postives = 210/376 (55.85%), Query Frame = 2
Query: 233 RVGLYRIKGNLGNGNFSQVKLAVHLLTREEVAIKILDKTKLDAKTQKLLSREISSMERLHHPNIIRLYEVIEDIRKMHLVMEYASGGELHSLIVSHGKIPESISKLIFAQIIAAINHLHSQNVIHRDLKAENIFFAGNKSETNQDNSKLNSTTKKSDFTDNAFSRLVHVKLG---DFGFSTLAQPAEKLTTFCGSPPYAAPELFQADWYYGTCVDIWALGILLYFMLCGCMPFRGESVGLLKKLILAGNYVCPNWLPVDAKNLIQQILKQNPDQRLTIQQIKNHQWLSGQHFPDAYQPFKATPLMPEIETISHAXXXXXXXXXXXXPLEKDSIEASKMLLRLGITDKHIEVAQAQTVRNYVTGTYRIMLHRVHRNR 1351
RVG Y I +G GNFS VKLA H +T+ +VAIKI+DKT+L+ K + RE+ M+ L HP+I++LY+V+E ++LV +YA+ GE+ + HG++PE ++ F QI++A+++ H ++V+HRDLKAEN+ N N K+ + ++H L DFGF +P L T+CGSPPYAAPE Y VD W LG++LY ++CG +PF G ++ L+ +L G + P ++ + ++LI+ +L ++P+QR TI+QI+ H+WL D+ + + M E E IS +E + +A +++ LGI K + A ++ T Y +++ R+ +R
Sbjct: 8 RVGFYEIDKTIGKGNFSVVKLARHRITKSQVAIKIIDKTQLNEMNLKKIYREVQIMKLLQHPHIVKLYQVMETKNMLYLVTDYANNGEMFDYLAHHGRLPEKEARKKFVQILSAVDYCHKRHVVHRDLKAENLLLDQNM------NIKIAGMS--------GLYLVIHCILFSSLDFGFGNYYKPGNPLNTWCGSPPYAAPEHI----YCEPKVDFWPLGVVLYVLVCGALPFDGSTLQALRDRVLEGKFRIPFFMSTECEHLIRHMLVKDPNQRYTIEQIQKHKWLQA---ADSQKISEIEQSMREFEKIS---------------MEDYNEQALELMKGLGIDIKRTKKALDDNTFDHHTAIYYLLVDRLRLHR 347
BLAST of Serine/threonine-protein kinase NIM1 vs. Ensembl Nematostella
Match: EDO36587 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SI81]) HSP 1 Score: 205.682 bits (522), Expect = 2.482e-61 Identity = 112/304 (36.84%), Postives = 173/304 (56.91%), Query Frame = 2
Query: 239 GLYRIKGNLGNGNFSQVKLAVHLLTREEVAIKILDKTKLDAKTQKLLSREISSMERLHHPNIIRLYEVIEDIRKMHLVMEYASGGELHSLIVSHGK-IPESISKLIFAQIIAAINHLHSQNVIHRDLKAENIFFAGNKSETNQDNSKLNSTTKKSDFTDNAFSRLVHVKLGDFGFSTLAQPAEKLTTFCGSPPYAAPELFQADWYYGTCVDIWALGILLYFMLCGCMPFRGESVGLLKKLILAGNYVCPNWLPVDAKNLIQQILKQNPDQRLTIQQIKNHQWLSGQHFPDAYQPFKATPLMPEI 1147
G+Y + LG G+F+ VK+A H++T E VA+K++DKTKLD +++ L +E+ M+ + HPN++RLY+VI+ K++L++E GG+++ I++H K +PE ++ F QI+ AI++ H +V+HRDLK EN+ F NQD +KL DFGFS P EKL T CGS Y+APE+ D Y VD+W+LG++LY ++CG PF + I+ Y P+ + KNLI ++L + P R ++ +I +H WL Q P P PL+ E+
Sbjct: 17 GMYDLGETLGRGHFAVVKVARHVITGERVAVKVIDKTKLDEVSREHLLKEVRCMKLVQHPNVVRLYQVIDTNTKLYLILELGDGGDMYDYIMNHDKGLPEEKARYFFRQIVLAIDYCHKLHVVHRDLKPENVIFF-----KNQDMAKLT----------------------DFGFSNNFIPNEKLDTACGSLAYSAPEVLLGDAYEAPAVDVWSLGVILYMLVCGQAPFSEANDSETLTKIMDCRYDVPDHVSPLCKNLISRMLIREPHNRASLGEIMSHPWL--QQGPTPLVP-SDVPLVAEL 290
BLAST of Serine/threonine-protein kinase NIM1 vs. Ensembl Medaka
Match: nim1k (NIM1 serine/threonine protein kinase [Source:NCBI gene;Acc:101164566]) HSP 1 Score: 380.948 bits (977), Expect = 5.930e-127 Identity = 187/403 (46.40%), Postives = 255/403 (63.28%), Query Frame = 2
Query: 161 TSFDKYTEEMNCNENVKKEIMLGKRVGLYRIKGNLGNGNFSQVKLAVHLLTREEVAIKILDKTKLDAKTQKLLSREISSMERLHHPNIIRLYEVIEDIRKMHLVMEYASGGELHSLIVSHGKIPESISKLIFAQIIAAINHLHSQNVIHRDLKAENIFFAGNKSETNQDNSKLNSTTKKSDFTDNAFSRLVHVKLGDFGFSTLAQPAEKLTTFCGSPPYAAPELFQADWYYGTCVDIWALGILLYFMLCGCMPFRGESVGLLKKLILAGNYVCPNWLPVDAKNLIQQILKQNPDQRLTIQQIKNHQWLSGQHFPDAYQPFKATPLMPEIETISHAXXXXXXXXXXXXPLEKDSIEASKMLLRLGITDKHIEVAQAQTVRNYVTGTYRIMLHRVHRNRRMSNIP 1369
T ++ +M ++ V +E+ +G+R+ Y+I+G +G GNFS VKL +H LT+++VAIKILDKTKLD KTQ+LLSREISSME+LHHPNIIRLYEV+E + ++HLVMEYA GGEL++ I + GK+ + K++FAQII+A+ H+H N+IHRDLKAENIF+ VK+GDFGFSTL+ E L TFCGSPPYAAPELF+ + Y G VDIWALG+LL+FM+ G MPFR E+V LK+ I+ G Y+ P W+P + LI+ IL+ P R T++Q+ + +WL FP + PF P +H + L ++ +E L LGIT +HI Q + R+ +TG YRI+LHRVHR R + +P
Sbjct: 78 TPLERLNLDMCQDDRVVRELTVGRRIAFYKIRGEIGCGNFSHVKLGIHALTKDKVAIKILDKTKLDQKTQRLLSREISSMEKLHHPNIIRLYEVVETLSRLHLVMEYAGGGELYTKITTEGKLSDLDCKIVFAQIISAVKHMHENNIIHRDLKAENIFYTSTSC----------------------------VKVGDFGFSTLSHRDETLNTFCGSPPYAAPELFRDEHYIGAFVDIWALGVLLFFMVTGTMPFRAETVVKLKRCIMDGAYIMPTWVPEACQRLIRGILQPVPSDRCTVEQMMSSEWLLPVDFPRSLDPFSLDP--------THLAESDPSE------LREEELEIKTALEALGITSEHILNNQGKDCRSSITGVYRILLHRVHRRRAVDIVP 438
BLAST of Serine/threonine-protein kinase NIM1 vs. Ensembl Medaka
Match: ENSORLT00000034173.1 (serine/threonine-protein kinase NIM1-like [Source:NCBI gene;Acc:101159907]) HSP 1 Score: 344.354 bits (882), Expect = 5.349e-113 Identity = 178/432 (41.20%), Postives = 256/432 (59.26%), Query Frame = 2
Query: 95 MNKKNSLDILPKISETEENNVETSFDKYTEEMNCNENVKKEIMLGKRVGLYRIKGNLGNGNFSQVKLAVHLLTREEVAIKILDKTKLDAKTQKLLSREISSMERLHHPNIIRLYEVIEDIRKMHLVMEYASGGELHSLIVSHGKIPESISKLIFAQIIAAINHLHSQNVIHRDLKAENIFFAGNKSETNQDNSKLNSTTKKSDFTDNAFSRLVHVKLGDFGFSTLAQPAEKLTTFCGSPPYAAPELFQADWYYGTCVDIWALGILLYFMLCGCMPFRGESVGLLKKLILAGNYVCPNWLPVDAKNLIQQILKQNPDQRLTIQQIKNHQWLSGQHFPDAYQPFKATPLMPEIETISHAXXXXXXXXXXXXPLEKDSIEASKMLLRLGITDKHIEVAQAQTVRNYVTGTYRIMLHRVHRNRRMSNIPHSLSNPE 1390
++ ++ +P+++E E +T ++ +M N+ V E++LG+RV Y ++G +G GNFS VKL +H LT+E VA+KI+DKT+LD K+Q L S EIS ME+L HPNI+RLYEVI+ RK++LVMEY SGG+L S I + GK+ + +KL+FAQII+AI H+H N++HRDLK ENIF+ + +K+GDFGFST + P E LT FCGSPPYAAPELF+ Y G DIWALG+LLYFM+ +PF GE++G LK+ IL G Y P ++P + +I+ +L+ P R T+ QI + WL G +P YQ +P SH L + E ++ LGI +++ Q R+ +TGTYRI+LHR + R + + +S+ +PE
Sbjct: 36 LSPSSAAGAVPQLTEAERLK-QTPLERVIYDMAHNDRVVNELILGRRVSFYELRGEIGQGNFSTVKLGIHALTKERVAVKIVDKTRLDKKSQPLTSSEISCMEKLSHPNIVRLYEVIDTSRKLYLVMEYGSGGDLFSRITTRGKLNDLEAKLVFAQIISAIKHMHDSNIVHRDLKPENIFYTTSYC----------------------------IKVGDFGFSTQSGPGELLTHFCGSPPYAAPELFRQKGYVGFYADIWALGVLLYFMVTATLPFFGENLGRLKRCILQGAYSIPAYVPEPCQLVIKGMLRIVPADRCTVPQITDSTWLKGIDYPGPYQLLPGSP--------SHFAQPGRV-------LSPEEQEVKNLMSTLGILAVNLQNNQCLDCRSPLTGTYRILLHRAQKRRSVQAVGYSVLHPE 423
BLAST of Serine/threonine-protein kinase NIM1 vs. Ensembl Medaka
Match: ENSORLT00000042211.1 (serine/threonine-protein kinase NIM1-like [Source:NCBI gene;Acc:101159907]) HSP 1 Score: 344.739 bits (883), Expect = 1.606e-112 Identity = 178/432 (41.20%), Postives = 256/432 (59.26%), Query Frame = 2
Query: 95 MNKKNSLDILPKISETEENNVETSFDKYTEEMNCNENVKKEIMLGKRVGLYRIKGNLGNGNFSQVKLAVHLLTREEVAIKILDKTKLDAKTQKLLSREISSMERLHHPNIIRLYEVIEDIRKMHLVMEYASGGELHSLIVSHGKIPESISKLIFAQIIAAINHLHSQNVIHRDLKAENIFFAGNKSETNQDNSKLNSTTKKSDFTDNAFSRLVHVKLGDFGFSTLAQPAEKLTTFCGSPPYAAPELFQADWYYGTCVDIWALGILLYFMLCGCMPFRGESVGLLKKLILAGNYVCPNWLPVDAKNLIQQILKQNPDQRLTIQQIKNHQWLSGQHFPDAYQPFKATPLMPEIETISHAXXXXXXXXXXXXPLEKDSIEASKMLLRLGITDKHIEVAQAQTVRNYVTGTYRIMLHRVHRNRRMSNIPHSLSNPE 1390
++ ++ +P+++E E +T ++ +M N+ V E++LG+RV Y ++G +G GNFS VKL +H LT+E VA+KI+DKT+LD K+Q L S EIS ME+L HPNI+RLYEVI+ RK++LVMEY SGG+L S I + GK+ + +KL+FAQII+AI H+H N++HRDLK ENIF+ + +K+GDFGFST + P E LT FCGSPPYAAPELF+ Y G DIWALG+LLYFM+ +PF GE++G LK+ IL G Y P ++P + +I+ +L+ P R T+ QI + WL G +P YQ +P SH L + E ++ LGI +++ Q R+ +TGTYRI+LHR + R + + +S+ +PE
Sbjct: 80 LSPSSAAGAVPQLTEAERLK-QTPLERVIYDMAHNDRVVNELILGRRVSFYELRGEIGQGNFSTVKLGIHALTKERVAVKIVDKTRLDKKSQPLTSSEISCMEKLSHPNIVRLYEVIDTSRKLYLVMEYGSGGDLFSRITTRGKLNDLEAKLVFAQIISAIKHMHDSNIVHRDLKPENIFYTTSYC----------------------------IKVGDFGFSTQSGPGELLTHFCGSPPYAAPELFRQKGYVGFYADIWALGVLLYFMVTATLPFFGENLGRLKRCILQGAYSIPAYVPEPCQLVIKGMLRIVPADRCTVPQITDSTWLKGIDYPGPYQLLPGSP--------SHFAQPGRV-------LSPEEQEVKNLMSTLGILAVNLQNNQCLDCRSPLTGTYRILLHRAQKRRSVQAVGYSVLHPE 467
BLAST of Serine/threonine-protein kinase NIM1 vs. Ensembl Medaka
Match: si:ch211-22d5.2 (serine/threonine-protein kinase NIM1-like [Source:NCBI gene;Acc:101156106]) HSP 1 Score: 333.954 bits (855), Expect = 4.875e-109 Identity = 176/416 (42.31%), Postives = 249/416 (59.86%), Query Frame = 2
Query: 107 NSLDILPKISETEENNVETSFDKYTEEMNCNENVKKEIMLGKRVGLYRIKGNLGNGNFSQVKLAVHLLTREEVAIKILDKTKLDAKTQKLLSREISSMERLHHPNIIRLYEVIEDIRKMHLVMEYASGGELHSLIVSHGKIPESISKLIFAQIIAAINHLHSQNVIHRDLKAENIFFAGNKSETNQDNSKLNSTTKKSDFTDNAFSRLVHVKLGDFGFST-LAQPAEKLTTFCGSPPYAAPELFQADWYYGTCVDIWALGILLYFMLCGCMPFRGESVGLLKKLILAGNYVCPNWLPVDAKNLIQQILKQNPDQRLTIQQIKNHQWLSGQHFPDAYQPFKATPLMPEIETISHAXXXXXXXXXXXXPLEKDSIEASKMLLRLGITDKHIEVAQAQTVRNYVTGTYRIMLHRVHRNR 1351
+S D P+ E T K T +M +E KE+++G+RVG Y+++G +G G FS+VKLA H LTR++VA+KILD+ +LDA+ Q+LLSREI+SME L HPNI+RLYEV+E +++LV+EYA GG+LH+ I S GK+ ++ SK+ FAQ+++AI ++H+ ++IHRDLKAEN+ F + + VK+ DFGFST ++ L TFCGSPPYAAPELF+ + Y G VD+WA+G+LL+FM+ G MPFR E++G L++ I+ GNY P W+P + LI+ ILK P +R I Q+ WL FP + P PE S + S L + E L LG T +H++ Q R+ VTG YRI+LHR + R
Sbjct: 22 DSSDCSPEDEEASPEPPLTPLQKLTADMCKDEKTIKELIIGRRVGFYKVRGEIGYGTFSRVKLAFHALTRDKVALKILDRMRLDAQAQRLLSREINSMEALQHPNIVRLYEVVETPSRLYLVLEYAGGGDLHNRICSEGKLCDNTSKVTFAQVLSAIKYMHNLSIIHRDLKAENVLFTSSGA----------------------------VKVADFGFSTRVSNRNLALDTFCGSPPYAAPELFRDESYLGPPVDVWAMGVLLFFMVTGTMPFRAETMGKLRRCIIEGNYTLPPWVPGPCQRLIRGILKPAPAERYAIDQMLGCDWLLPVEFPWSLVP-------PEPTNALQRLLDSERSSSVEAELHE---EVRSSLQELGFTSEHLQNNQLTDSRSPVTGVYRILLHRSQKRR 399
BLAST of Serine/threonine-protein kinase NIM1 vs. Ensembl Medaka
Match: si:ch211-22d5.2 (serine/threonine-protein kinase NIM1-like [Source:NCBI gene;Acc:101156106]) HSP 1 Score: 333.954 bits (855), Expect = 4.875e-109 Identity = 176/416 (42.31%), Postives = 249/416 (59.86%), Query Frame = 2
Query: 107 NSLDILPKISETEENNVETSFDKYTEEMNCNENVKKEIMLGKRVGLYRIKGNLGNGNFSQVKLAVHLLTREEVAIKILDKTKLDAKTQKLLSREISSMERLHHPNIIRLYEVIEDIRKMHLVMEYASGGELHSLIVSHGKIPESISKLIFAQIIAAINHLHSQNVIHRDLKAENIFFAGNKSETNQDNSKLNSTTKKSDFTDNAFSRLVHVKLGDFGFST-LAQPAEKLTTFCGSPPYAAPELFQADWYYGTCVDIWALGILLYFMLCGCMPFRGESVGLLKKLILAGNYVCPNWLPVDAKNLIQQILKQNPDQRLTIQQIKNHQWLSGQHFPDAYQPFKATPLMPEIETISHAXXXXXXXXXXXXPLEKDSIEASKMLLRLGITDKHIEVAQAQTVRNYVTGTYRIMLHRVHRNR 1351
+S D P+ E T K T +M +E KE+++G+RVG Y+++G +G G FS+VKLA H LTR++VA+KILD+ +LDA+ Q+LLSREI+SME L HPNI+RLYEV+E +++LV+EYA GG+LH+ I S GK+ ++ SK+ FAQ+++AI ++H+ ++IHRDLKAEN+ F + + VK+ DFGFST ++ L TFCGSPPYAAPELF+ + Y G VD+WA+G+LL+FM+ G MPFR E++G L++ I+ GNY P W+P + LI+ ILK P +R I Q+ WL FP + P PE S + S L + E L LG T +H++ Q R+ VTG YRI+LHR + R
Sbjct: 22 DSSDCSPEDEEASPEPPLTPLQKLTADMCKDEKTIKELIIGRRVGFYKVRGEIGYGTFSRVKLAFHALTRDKVALKILDRMRLDAQAQRLLSREINSMEALQHPNIVRLYEVVETPSRLYLVLEYAGGGDLHNRICSEGKLCDNTSKVTFAQVLSAIKYMHNLSIIHRDLKAENVLFTSSGA----------------------------VKVADFGFSTRVSNRNLALDTFCGSPPYAAPELFRDESYLGPPVDVWAMGVLLFFMVTGTMPFRAETMGKLRRCIIEGNYTLPPWVPGPCQRLIRGILKPAPAERYAIDQMLGCDWLLPVEFPWSLVP-------PEPTNALQRLLDSERSSSVEAELHE---EVRSSLQELGFTSEHLQNNQLTDSRSPVTGVYRILLHRSQKRR 399
BLAST of Serine/threonine-protein kinase NIM1 vs. Planmine SMEST
Match: SMESG000005159.1 (SMESG000005159.1) HSP 1 Score: 973.385 bits (2515), Expect = 0.000e+0 Identity = 480/480 (100.00%), Postives = 480/480 (100.00%), Query Frame = 2
Query: 95 MNKKNSLDILPKISETEENNVETSFDKYTEEMNCNENVKKEIMLGKRVGLYRIKGNLGNGNFSQVKLAVHLLTREEVAIKILDKTKLDAKTQKLLSREISSMERLHHPNIIRLYEVIEDIRKMHLVMEYASGGELHSLIVSHGKIPESISKLIFAQIIAAINHLHSQNVIHRDLKAENIFFAGNKSETNQDNSKLNSTTKKSDFTDNAFSRLVHVKLGDFGFSTLAQPAEKLTTFCGSPPYAAPELFQADWYYGTCVDIWALGILLYFMLCGCMPFRGESVGLLKKLILAGNYVCPNWLPVDAKNLIQQILKQNPDQRLTIQQIKNHQWLSGQHFPDAYQPFKATPLMPEIETISHAXXXXXXXXXXXXPLEKDSIEASKMLLRLGITDKHIEVAQAQTVRNYVTGTYRIMLHRVHRNRRMSNIPHSLSNPELGRKFFNSRKFQSKVITRKVNGAGPKPKTSSCPEKGIEPNQKSSKMCN 1534
MNKKNSLDILPKISETEENNVETSFDKYTEEMNCNENVKKEIMLGKRVGLYRIKGNLGNGNFSQVKLAVHLLTREEVAIKILDKTKLDAKTQKLLSREISSMERLHHPNIIRLYEVIEDIRKMHLVMEYASGGELHSLIVSHGKIPESISKLIFAQIIAAINHLHSQNVIHRDLKAENIFFAGNKSETNQDNSKLNSTTKKSDFTDNAFSRLVHVKLGDFGFSTLAQPAEKLTTFCGSPPYAAPELFQADWYYGTCVDIWALGILLYFMLCGCMPFRGESVGLLKKLILAGNYVCPNWLPVDAKNLIQQILKQNPDQRLTIQQIKNHQWLSGQHFPDAYQPFKATPLMPEIETISHADDISIDTISEIDPLEKDSIEASKMLLRLGITDKHIEVAQAQTVRNYVTGTYRIMLHRVHRNRRMSNIPHSLSNPELGRKFFNSRKFQSKVITRKVNGAGPKPKTSSCPEKGIEPNQKSSKMCN
Sbjct: 1 MNKKNSLDILPKISETEENNVETSFDKYTEEMNCNENVKKEIMLGKRVGLYRIKGNLGNGNFSQVKLAVHLLTREEVAIKILDKTKLDAKTQKLLSREISSMERLHHPNIIRLYEVIEDIRKMHLVMEYASGGELHSLIVSHGKIPESISKLIFAQIIAAINHLHSQNVIHRDLKAENIFFAGNKSETNQDNSKLNSTTKKSDFTDNAFSRLVHVKLGDFGFSTLAQPAEKLTTFCGSPPYAAPELFQADWYYGTCVDIWALGILLYFMLCGCMPFRGESVGLLKKLILAGNYVCPNWLPVDAKNLIQQILKQNPDQRLTIQQIKNHQWLSGQHFPDAYQPFKATPLMPEIETISHADDISIDTISEIDPLEKDSIEASKMLLRLGITDKHIEVAQAQTVRNYVTGTYRIMLHRVHRNRRMSNIPHSLSNPELGRKFFNSRKFQSKVITRKVNGAGPKPKTSSCPEKGIEPNQKSSKMCN 480
BLAST of Serine/threonine-protein kinase NIM1 vs. Planmine SMEST
Match: SMESG000055722.1 (SMESG000055722.1) HSP 1 Score: 255.758 bits (652), Expect = 1.093e-74 Identity = 127/368 (34.51%), Postives = 217/368 (58.97%), Query Frame = 2
Query: 236 VGLYRIKGNLGNGNFSQVKLAVHLLTREEVAIKILDKTKLDAKTQKLLSREISSMERLHHPNIIRLYEVIEDIRKMHLVMEYASGGELHSLIVSHGKIPESISKLIFAQIIAAINHLHSQNVIHRDLKAENIFFAGNKSETNQDNSKLNSTTKKSDFTDNAFSRLVHVKLGDFGFSTLAQPAEKLTTFCGSPPYAAPELFQADWYYGTCVDIWALGILLYFMLCGCMPFRGESVGLLKKLILAGNYVCPNWLPVDAKNLIQQILKQNPDQRLTIQQIKNHQWLSGQHFPDAYQPFKATPLMPEIETISHAXXXXXXXXXXXXPLEKDSIEASKMLLRLGITDKHIEVAQAQTVRNYVTGTYRIMLHRV 1339
VG Y+ +G GNF++VKLA H++T++EVAIKI+DK+ L+ +++ L+RE+ M+ L+HPNI++L+E+IE+ + ++LVMEYASGGE+ +V+HG++ E ++L F QI++A+ + H + ++HRDLKAEN+ + +++K+ DFGFS +P +KL TFCGSPPYAAPELFQ Y G VD+W+LG++LY ++ G +PF G+++ L++ +L G Y P ++ D ++L++++L NP +R +++ + +W++ F+ L P IE P D + ++++ +G T IE + + + +T TY ++ R
Sbjct: 52 VGKYKFIRTIGKGNFAKVKLAKHVITQKEVAIKIIDKSHLNPSSKQKLNREVKIMKCLNHPNIVKLFEIIENDKILYLVMEYASGGEVFDYLVAHGRMKEKEARLKFRQIVSAVQYCHQKKIVHRDLKAENLL----------------------------LDKEMNIKIADFGFSNEFKPGDKLDTFCGSPPYAAPELFQGKKYDGPEVDVWSLGVILYTLVSGSLPFDGQNLRDLRERVLKGKYRIPFYMSTDCESLLRKMLVLNPQKRYSLEAVMKDRWMN--------IGFEENQLTPYIE---------------PPPNYHDPVRY-ELMVNMGFTHAEIENSLTEGNFDNITATYFLLEERT 367
BLAST of Serine/threonine-protein kinase NIM1 vs. Planmine SMEST
Match: SMESG000055722.1 (SMESG000055722.1) HSP 1 Score: 255.373 bits (651), Expect = 1.446e-74 Identity = 127/368 (34.51%), Postives = 217/368 (58.97%), Query Frame = 2
Query: 236 VGLYRIKGNLGNGNFSQVKLAVHLLTREEVAIKILDKTKLDAKTQKLLSREISSMERLHHPNIIRLYEVIEDIRKMHLVMEYASGGELHSLIVSHGKIPESISKLIFAQIIAAINHLHSQNVIHRDLKAENIFFAGNKSETNQDNSKLNSTTKKSDFTDNAFSRLVHVKLGDFGFSTLAQPAEKLTTFCGSPPYAAPELFQADWYYGTCVDIWALGILLYFMLCGCMPFRGESVGLLKKLILAGNYVCPNWLPVDAKNLIQQILKQNPDQRLTIQQIKNHQWLSGQHFPDAYQPFKATPLMPEIETISHAXXXXXXXXXXXXPLEKDSIEASKMLLRLGITDKHIEVAQAQTVRNYVTGTYRIMLHRV 1339
VG Y+ +G GNF++VKLA H++T++EVAIKI+DK+ L+ +++ L+RE+ M+ L+HPNI++L+E+IE+ + ++LVMEYASGGE+ +V+HG++ E ++L F QI++A+ + H + ++HRDLKAEN+ + +++K+ DFGFS +P +KL TFCGSPPYAAPELFQ Y G VD+W+LG++LY ++ G +PF G+++ L++ +L G Y P ++ D ++L++++L NP +R +++ + +W++ F+ L P IE P D + ++++ +G T IE + + + +T TY ++ R
Sbjct: 52 VGKYKFIRTIGKGNFAKVKLAKHVITQKEVAIKIIDKSHLNPSSKQKLNREVKIMKCLNHPNIVKLFEIIENDKILYLVMEYASGGEVFDYLVAHGRMKEKEARLKFRQIVSAVQYCHQKKIVHRDLKAENLL----------------------------LDKEMNIKIADFGFSNEFKPGDKLDTFCGSPPYAAPELFQGKKYDGPEVDVWSLGVILYTLVSGSLPFDGQNLRDLRERVLKGKYRIPFYMSTDCESLLRKMLVLNPQKRYSLEAVMKDRWMNIG--------FEENQLTPYIE---------------PPPNYHDPVRY-ELMVNMGFTHAEIENSLTEGNFDNITATYFLLEERT 367
BLAST of Serine/threonine-protein kinase NIM1 vs. Planmine SMEST
Match: SMESG000015305.1 (SMESG000015305.1) HSP 1 Score: 248.44 bits (633), Expect = 4.112e-72 Identity = 118/300 (39.33%), Postives = 190/300 (63.33%), Query Frame = 2
Query: 236 VGLYRIKGNLGNGNFSQVKLAVHLLTREEVAIKILDKTKLDAKTQKLLSREISSMERLHHPNIIRLYEVIEDIRKMHLVMEYASGGELHSLIVSHGKIPESISKLIFAQIIAAINHLHSQNVIHRDLKAENIFFAGNKSETNQDNSKLNSTTKKSDFTDNAFSRLVHVKLGDFGFSTLAQPAEKLTTFCGSPPYAAPELFQADWYYGTCVDIWALGILLYFMLCGCMPFRGESVGLLKKLILAGNYVCPNWLPVDAKNLIQQILKQNPDQRLTIQQIKNHQWLSGQHFPDAYQPFKATPL 1135
VG Y+ +G GNF++VKL VH+ T++EVAIKI+DKT+L+ ++ LSRE+ M+ L+HPNI++LYE+IE+ + ++LVMEYASGGE+ +V+HG++ E ++ F QI++A+ + H ++++HRDLKAEN+ DN +++K+ DFGF+ KL TFCGSPPYAAPELFQ Y G VD+W+LG++LY ++ G +PF G+++ L++ +L G Y P ++ D + L++++L N +R T+ I + W++ H + P++ P+
Sbjct: 51 VGKYKFIRTIGKGNFAKVKLGVHVTTQKEVAIKIIDKTQLNQSSKLKLSREVRIMKTLNHPNIVKLYEIIENDKILYLVMEYASGGEVFDYLVAHGRMKEKEARSKFRQILSAVQYCHQRHIVHRDLKAENLLL---------DND-------------------LNIKIADFGFANEFIEGNKLDTFCGSPPYAAPELFQGKKYDGPEVDVWSLGVILYTLVSGSLPFDGQNLRELRERVLRGKYRIPYYMSTDCEALLRKMLLLNSQKRHTLLSIMDDLWVNIGHENNKLVPYEEPPM 322
BLAST of Serine/threonine-protein kinase NIM1 vs. Planmine SMEST
Match: SMESG000042362.1 (SMESG000042362.1) HSP 1 Score: 240.736 bits (613), Expect = 1.458e-68 Identity = 119/308 (38.64%), Postives = 183/308 (59.42%), Query Frame = 2
Query: 227 GKRVGLYRIKGNLGNGNFSQVKLAVHLLTREEVAIKILDKTKLDAKTQKLLSREISSMERLHHPNIIRLYEVIEDIRKMHLVMEYASGGELHSLIVSHGKIPESISKLIFAQIIAAINHLHSQNVIHRDLKAENIFFAGNKSETNQDNSKLNSTTKKSDFTDNAFSRLVHVKLGDFGFSTLAQPAEKLTTFCGSPPYAAPELFQADWYYGTCVDIWALGILLYFMLCGCMPFRGESVGLLKKLILAGNYVCPNWLPVDAKNLIQQILKQNPDQRLTIQQIKNHQWLSGQHFPDAYQPFKATPLMPEIE 1150
G G Y++ +G GNF++VKLA HL+T +EVA+KI+DKTKL+A + L+RE+ M+ L+HPNI+RLYEVI+ R ++LVME A GE+ +V +G++ E ++ F QI++A+ + H + ++HRDLKAEN+ + + +K+ DFGFS KL TFCGSPPYAAPELFQ Y G VD+W+LG++LY ++ G +PF G ++ L++ +L G Y P ++ D + L++++L NP +RL + I W++ + +PF PEI+
Sbjct: 127 GPTFGKYKLIREIGKGNFAKVKLAQHLITGKEVAVKIIDKTKLNAPALQKLTREVKIMKELNHPNIVRLYEVIQTERSLYLVMENAQNGEVFEYLVKNGRMKEKDARAKFRQIVSAVQYCHQKMIVHRDLKAENLL----------------------------LDKDLSIKIADFGFSNHFSRQSKLNTFCGSPPYAAPELFQGRRYEGPEVDVWSLGVILYTLISGSLPFDGGTLRELRERVLRGKYRIPFYMSSDCEALLRKMLVLNPQRRLPLTDIMKDTWINVGYEECPLRPF----YEPEID 402 The following BLAST results are available for this feature:
BLAST of Serine/threonine-protein kinase NIM1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Serine/threonine-protein kinase NIM1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of Serine/threonine-protein kinase NIM1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of Serine/threonine-protein kinase NIM1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of Serine/threonine-protein kinase NIM1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of Serine/threonine-protein kinase NIM1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of Serine/threonine-protein kinase NIM1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Serine/threonine-protein kinase NIM1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Serine/threonine-protein kinase NIM1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of Serine/threonine-protein kinase NIM1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of Serine/threonine-protein kinase NIM1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 5
BLAST of Serine/threonine-protein kinase NIM1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of Serine/threonine-protein kinase NIM1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of Serine/threonine-protein kinase NIM1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30029249 ID=SMED30029249|Name=Serine/threonine-protein kinase NIM1|organism=Schmidtea mediterranea sexual|type=transcript|length=1692bpback to top protein sequence of SMED30029249-orf-1 >SMED30029249-orf-1 ID=SMED30029249-orf-1|Name=SMED30029249-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=483bp MNKKNSLDILPKISETEENNVETSFDKYTEEMNCNENVKKEIMLGKRVGLback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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