SMED30029205

Overview
NameSMED30029205
Smed IDSMED30029205
Length (bp)2412
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30029205 (SMED30029205) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30029205 (SMED30029205) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30029205 (SMED30029205) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30029205 (SMED30029205) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30029205

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 9

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30029205SMESG000040424.1 SmedASXL_008573SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
head regionSMED30029205SMESG000040424.1 SmedASXL_005500SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
head regionSMED30029205SMESG000040424.1 SmedASXL_019424SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
pharynxSMED30029205SMESG000040424.1 dd_Smed_v4_33167_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30029205SMESG000040424.1 dd_Smed_v4_33167_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30029205SMESG000040424.1 dd_Smed_v4_33167_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30029205SMESG000040424.1 dd_Smed_v4_33167_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30029205SMESG000040424.1 dd_Smed_v6_64866_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
head regionSMED30029205SMESG000040424.1 dd_Smed_v6_33167_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of SMED30029205 vs. Planmine SMEST
Match: SMESG000040424.1 (SMESG000040424.1)

HSP 1 Score: 977.237 bits (2525), Expect = 0.000e+0
Identity = 552/558 (98.92%), Postives = 556/558 (99.64%), Query Frame = 2
Query:  662 AESCGYIQPDITWIIVNSCXXXXXXXXXXXXXINFISFFDSRFSNQIYPDCFIKKGEVVIKYIIDNLNSLTNSTDLFNLIQPHKCNIERPITNKSFFTEIFSEFKKSFLAGQNGYLNIVKTQLIDPNNASVSTSELYRGFKFENISLRVCVRQAPTLYHNSTTGFIPRILTKLYEKFNFNWSIVTPETGNMENCNEXXXXXXXXXXXXFASILDTSDLPIVFWTILEEEHCFILGVDPVDYGVTLWAPVQFFNWTFLLLLGGCLVIYSLLALIFTKFNPPIVDSNSTMPALTADSSFLDFCWNLTGAFLAQGLEYNPLNPSLSTLXXXXXXXXXXXXXXXXGSLTVTMTVTNNRYSFLSNSIFELLKTERVDVFIVRGSYIDKWLNNSTENVYSLLKSRKIYVSTLEECIQNVLNHISSKSPQLAVCLAPCINTMTPREICDKMRMLSDTLFPISISLGFHEMSPWKNFIISELNTMKFMGVMEQFRHDSISNINCLPQNQNDQFSGQSXXXXXXXXXXXXXXALIFMGIICLGLEIIYKNLKKKELIKRSIFSKHFQ 2335
            AESCGYIQPDITWIIVNSCLPPFSLSLPLYSSINF+SFFDSRFSNQIYPDCFIKKGEVVIKYIIDNL+SLTNSTDLFNLIQPHKCNIERPITNKSFFTEIFSEFKKSFLAGQNGYLNI KTQLIDPNNASVSTSELYRGFKFENISL VCVRQAPTLYHNSTTGFIPRILTKLYEKFNFNWSIVTPETGNMENCNEKLVKKLVDVSVDFASILDTSDLPIVFWTILEEEHCFILGVDPVDYGVTLWAPVQFFNWTFLLLLGGCL+IYSLLALIFTKFNPPIVDSNSTMPALTADSSFLDFCWNLTGAFLAQGLEYNPLNPSLSTLSFFWWVYSIIISSVYSGSLTVTMTVTNNRYSFLSNSIFELLKTERVDVFIVRGSYIDKWLNNSTENVYSLLKSRKIYVSTLEECIQNVLNHISSKSPQLAVCLAPCINTMTPREICDKMRMLSDTLFPISISLGFHEMSPWKNFIISELNTMKFMGVMEQFRHDSISNINCLPQNQNDQFSGQSISISGLIGVIYLIYALIFMGIICLGLEI+YKNLKKKELIKRSIFSKHFQ
Sbjct:  207 AESCGYIQPDITWIIVNSCLPPFSLSLPLYSSINFVSFFDSRFSNQIYPDCFIKKGEVVIKYIIDNLHSLTNSTDLFNLIQPHKCNIERPITNKSFFTEIFSEFKKSFLAGQNGYLNIEKTQLIDPNNASVSTSELYRGFKFENISLSVCVRQAPTLYHNSTTGFIPRILTKLYEKFNFNWSIVTPETGNMENCNEKLVKKLVDVSVDFASILDTSDLPIVFWTILEEEHCFILGVDPVDYGVTLWAPVQFFNWTFLLLLGGCLIIYSLLALIFTKFNPPIVDSNSTMPALTADSSFLDFCWNLTGAFLAQGLEYNPLNPSLSTLSFFWWVYSIIISSVYSGSLTVTMTVTNNRYSFLSNSIFELLKTERVDVFIVRGSYIDKWLNNSTENVYSLLKSRKIYVSTLEECIQNVLNHISSKSPQLAVCLAPCINTMTPREICDKMRMLSDTLFPISISLGFHEMSPWKNFIISELNTMKFMGVMEQFRHDSISNINCLPQNQNDQFSGQSISISGLIGVIYLIYALIFMGIICLGLEIVYKNLKKKELIKRSIFSKHFQ 764          

HSP 2 Score: 407.527 bits (1046), Expect = 1.848e-130
Identity = 202/205 (98.54%), Postives = 202/205 (98.54%), Query Frame = 3
Query:    3 TNLFFDAVLCGIWGRILCQRIGLLSASKGLLSNFQNDNRIFTFYVNTSDIFELKNIVCQENFQSLIFDSLPPESESKLVFITSFFQIPLFWISSTWPWKYTAKNDNSIIFQHNLLDLAEIVVKYIREYTISETLLVGDEFSRNLFLLVFNLISQTRVELITGKELTGENCSSRMRSVLIRMKWKLQIIVLTEPGSVPFLLRKKNS 617
            TNLFFDAVLCGIWGRILCQRIGLLSASKGLLSNFQNDNRIFTFYVNTSDIFELKNIVCQENFQSLIFDSLPPESESKLVFITSFFQIPLFWISSTWPWKYTAKNDNSIIFQHNLLDLAEIVVKYIREYTISETLLVGDEFSRNLFLLVFNLISQTRVELITGKELTGENCSSRMRSVLIRMKWKLQIIVL EPGSVPFLLRK  S
Sbjct:    5 TNLFFDAVLCGIWGRILCQRIGLLSASKGLLSNFQNDNRIFTFYVNTSDIFELKNIVCQENFQSLIFDSLPPESESKLVFITSFFQIPLFWISSTWPWKYTAKNDNSIIFQHNLLDLAEIVVKYIREYTISETLLVGDEFSRNLFLLVFNLISQTRVELITGKELTGENCSSRMRSVLIRMKWKLQIIVLAEPGSVPFLLRKAES 209          
BLAST of SMED30029205 vs. Planmine SMEST
Match: SMESG000018611.1 (SMESG000018611.1)

HSP 1 Score: 61.6178 bits (148), Expect = 2.162e-9
Identity = 69/288 (23.96%), Postives = 131/288 (45.49%), Query Frame = 2
Query: 1328 WTILEEEHCFILGVDPVDYGVTLWAPVQFFNWTFLLLLGGCLVIYSLLALIFTKFNPPIVDSNSTMPALTADSSFLDFCWNLTGAFLAQGLEYNPLNPSLSTLXXXXXXXXXXXXXXXXGSLTVTMTVTNNRYSFLSNSIFELLKTERVDVFIVRGSYIDKWLNNSTENVYSLLKSRKIYVSTLEECIQNVLNHISSKSPQLAVCLAP-CINTMTPREICDKMRMLSDTLFPISISLGFHEMSPWKNFIISELNTMKFMGVMEQ-----FRHDSISNINCLPQNQNDQ 2173
            + I+EE +  +L          +W   + FNW+  LL    + + S    +F+K+NP    + +   A+  +    +  WN  G F+ QG ++ P   S   +   WW++ +II S Y+  LT  +TV+    S LS S+  L     + + I +G+ +D  L  ST   Y  L SRKIY+ T++ C + +L   S+      VC+    +   T  + C  +  + +T     +     + +P+K  I   + ++   G++ +     ++ +   + NC  +N N++
Sbjct:  638 YPIMEESNVPVLQKPKTQ--TKMWHIFRPFNWSVWLLSIATIFLISYFCYVFSKYNPLSAYNLNLNFAIVDEIYIKEHLWNAIGGFVLQGQDFYPYTVSARIVLAIWWLFCLIIFSAYTADLTSFLTVSEGN-SLLS-SLDALAARTDLKIVIPKGTNLDALLIESTNPTYQALNSRKIYIDTIDNCPKKMLELSSTD----LVCITDTSVVVYTVNKYCSVIYTVPETFNTAPLGFLTPKDAPFKKKIDQIIQSLVETGIISRWIEFYWKTNQSDSQNCEAENPNEE 917          

HSP 2 Score: 52.7582 bits (125), Expect = 1.405e-6
Identity = 63/254 (24.80%), Postives = 120/254 (47.24%), Query Frame = 2
Query: 1403 PVQFFNWTFLLLLGGCLVIYSLLALIFTKFNPPIVDSNSTMPALTADSSFLDFCWNLTGAFLAQGLEYNPLNPSLSTLXXXXXXXXXXXXXXXXGSLTVTMTVTNNRYSFLSNSIFELLKTERVDVFIVRGSYIDKWLNNSTENVYSLLKSRKIYVSTLEECIQNVLNHISSKSPQLAVCLAPCINTMTPREICDKMRMLSDTLFPISISLGFHEMSPWKNFIISELNTMKFMG-----VMEQFRHDSISNINC 2149
            P+QF  W  +L+    +   SL    F+KF+P    + +   A+  +    +  WN+ G+   QG ++ P   S   +   WW++++II S YS  LT  +TV   + +F+S+ I EL   + + + + +G+ ID  L  S   VY  L  RKI +  +++C + +L  + S +  + +     +  +T    C  + M+ +     ++     + SP+KN I S +  +   G     V + +  D +++ NC
Sbjct:  105 PLQFSVWMLILV---SVAFVSLCCFSFSKFSPLSAWNLNLNFAIIDEVKLKENTWNVIGSLFMQGQDFYPFCISSRIVLAIWWIHALIIFSAYSADLTSFLTV-QEKNNFISD-INELANRKDLYIIVPKGTNIDSSLAESHNAVYQELNRRKITIDNVDDCPKKIL--LPSSTNLVCISDRSGMRYVTNMN-CKDVYMVPEIFNIAALGFVLEKDSPYKNMIDSYIQILVENGLITKWVRKYWEDDEVTSRNC 350          
BLAST of SMED30029205 vs. Planmine SMEST
Match: SMESG000018611.1 (SMESG000018611.1)

HSP 1 Score: 60.8474 bits (146), Expect = 4.749e-9
Identity = 55/192 (28.65%), Postives = 93/192 (48.44%), Query Frame = 2
Query: 1328 WTILEEEHCFILGVDPVDYGVTLWAPVQFFNWTFLLLLGGCLVIYSLLALIFTKFNPPIVDSNSTMPALTADSSFLDFCWNLTGAFLAQGLEYNPLNPSLSTLXXXXXXXXXXXXXXXXGSLTVTMTVTNNRYSFLSNSIFELLKTERVDVFIVRGSYIDKWLNNSTENVYSLLKSRKIYVSTLEECIQNVL 1903
            + I+EE +  +L          +W   + FNW+  LL    + + S    +F+K+NP    + +   A+  +    +  WN  G F+ QG ++ P   S   +   WW++ +II S Y+  LT  +TV+    S LS S+  L     + + I +G+ +D  L  ST   Y  L SRKIY+ T++ C + +L
Sbjct:  638 YPIMEESNVPVLQKPKTQ--TKMWHIFRPFNWSVWLLSIATIFLISYFCYVFSKYNPLSAYNLNLNFAIVDEIYIKEHLWNAIGGFVLQGQDFYPYTVSARIVLAIWWLFCLIIFSAYTADLTSFLTVSEGN-SLLS-SLDALAARTDLKIVIPKGTNLDALLIESTNPTYQALNSRKIYIDTIDNCPKKML 825          

HSP 2 Score: 52.7582 bits (125), Expect = 1.132e-6
Identity = 63/254 (24.80%), Postives = 120/254 (47.24%), Query Frame = 2
Query: 1403 PVQFFNWTFLLLLGGCLVIYSLLALIFTKFNPPIVDSNSTMPALTADSSFLDFCWNLTGAFLAQGLEYNPLNPSLSTLXXXXXXXXXXXXXXXXGSLTVTMTVTNNRYSFLSNSIFELLKTERVDVFIVRGSYIDKWLNNSTENVYSLLKSRKIYVSTLEECIQNVLNHISSKSPQLAVCLAPCINTMTPREICDKMRMLSDTLFPISISLGFHEMSPWKNFIISELNTMKFMG-----VMEQFRHDSISNINC 2149
            P+QF  W  +L+    +   SL    F+KF+P    + +   A+  +    +  WN+ G+   QG ++ P   S   +   WW++++II S YS  LT  +TV   + +F+S+ I EL   + + + + +G+ ID  L  S   VY  L  RKI +  +++C + +L  + S +  + +     +  +T    C  + M+ +     ++     + SP+KN I S +  +   G     V + +  D +++ NC
Sbjct:  105 PLQFSVWMLILV---SVAFVSLCCFSFSKFSPLSAWNLNLNFAIIDEVKLKENTWNVIGSLFMQGQDFYPFCISSRIVLAIWWIHALIIFSAYSADLTSFLTV-QEKNNFISD-INELANRKDLYIIVPKGTNIDSSLAESHNAVYQELNRRKITIDNVDDCPKKIL--LPSSTNLVCISDRSGMRYVTNMN-CKDVYMVPEIFNIAALGFVLEKDSPYKNMIDSYIQILVENGLITKWVRKYWEDDEVTSRNC 350          
BLAST of SMED30029205 vs. Planmine SMEST
Match: SMESG000018611.1 (SMESG000018611.1)

HSP 1 Score: 52.7582 bits (125), Expect = 6.676e-7
Identity = 63/254 (24.80%), Postives = 120/254 (47.24%), Query Frame = 2
Query: 1403 PVQFFNWTFLLLLGGCLVIYSLLALIFTKFNPPIVDSNSTMPALTADSSFLDFCWNLTGAFLAQGLEYNPLNPSLSTLXXXXXXXXXXXXXXXXGSLTVTMTVTNNRYSFLSNSIFELLKTERVDVFIVRGSYIDKWLNNSTENVYSLLKSRKIYVSTLEECIQNVLNHISSKSPQLAVCLAPCINTMTPREICDKMRMLSDTLFPISISLGFHEMSPWKNFIISELNTMKFMG-----VMEQFRHDSISNINC 2149
            P+QF  W  +L+    +   SL    F+KF+P    + +   A+  +    +  WN+ G+   QG ++ P   S   +   WW++++II S YS  LT  +TV   + +F+S+ I EL   + + + + +G+ ID  L  S   VY  L  RKI +  +++C + +L  + S +  + +     +  +T    C  + M+ +     ++     + SP+KN I S +  +   G     V + +  D +++ NC
Sbjct:  105 PLQFSVWMLILV---SVAFVSLCCFSFSKFSPLSAWNLNLNFAIIDEVKLKENTWNVIGSLFMQGQDFYPFCISSRIVLAIWWIHALIIFSAYSADLTSFLTV-QEKNNFISD-INELANRKDLYIIVPKGTNIDSSLAESHNAVYQELNRRKITIDNVDDCPKKIL--LPSSTNLVCISDRSGMRYVTNMN-CKDVYMVPEIFNIAALGFVLEKDSPYKNMIDSYIQILVENGLITKWVRKYWEDDEVTSRNC 350          
BLAST of SMED30029205 vs. Planmine SMEST
Match: SMESG000080367.1 (SMESG000080367.1)

HSP 1 Score: 50.8322 bits (120), Expect = 3.697e-6
Identity = 82/392 (20.92%), Postives = 159/392 (40.56%), Query Frame = 2
Query: 1049 SVSTSELYRGFKFENISLRVC-VRQAP--TLYHNS-------TTGFIPRILTKLYEKFNFNWSIVTPETGN----------MENCNEXXXXXXXXXXXXFASILDTSDLPIVFWTILEEEHCFILGVDPVDYGVTLWAPVQFFNWTFLLLLGGCLVIYSLLALIFTKFNPPIVDSNSTMPALTADSSFLDFCWNLTGAFLAQGLEYNPLNPSLSTLXXXXXXXXXXXXXXXXGSLTVTMTVTNNRYSFLSNSIFELLKTERVDVFIVRGSYIDKWLNNSTENVYSLLKSRKIYVSTLEECIQNVLNHISSKSPQLAVCLAPCINTMTPREI-CDKMRMLSDTLFPISISLGFHEMSPWKNFIISELNTMKFMGVMEQFRHDSISNINCLPQN 2161
            S+ST++    + F N ++++C +   P  T Y+ S        +GF+ +I+  L ++F F + I  P  G              C  ++  K    +    +  D     I +      E   I+  +P+      W P   F  +   L  G L   S+LA++F ++ P    +     A+  +    +  W  TG F  QG  +     S   ++  WW+ + I+ S+Y+  L   +T+   R   L N++ E+  +E + + +  G  I  +L +S + V   L S+  +V    + + N   H          C+A        + + C +  +L + +    +   F ++  +KN    E+  +   GV+ ++  + +SN  C  QN
Sbjct:  349 SISTTK----YSFRNKTIKICSMEYVPFFTKYNKSLPYSKDNISGFLAKIIKHLAKEFQFQFEIYEPLDGQSGVRIKNKTIFSGCVGEVQTKNASFAASTFTFNDERMTVIDYSYPFMSESQIIIFQNPLPKS-HWWNPFLSFKVSVWALTFGSLAFVSILAMVFCRWGPFSAWNLKLQFAVRDEIQLGEHLWTSTGVFFMQGQNFYAYPISSRFVTASWWILTAILHSIYAADLASILTIDTERK--LPNTLQEVADSEDITLHLSSGYAITAYLKSSNDPVQQALYSKSKFVPQCYDLLLNAETHN-------VACIADNSQMSYIKSLNCSRFTILQEIVKSTLVGFFFPKLVDFKNDFDKEIQLLGDKGVIRKWFLEDLSNSTCGSQN 726          
The following BLAST results are available for this feature:
BLAST of SMED30029205 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30029205 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30029205 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30029205 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30029205 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30029205 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30029205 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30029205 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30029205 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30029205 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30029205 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30029205 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30029205 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30029205 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000040424.11.848e-13098.92SMESG000040424.1[more]
SMESG000018611.12.162e-923.96SMESG000018611.1[more]
SMESG000018611.14.749e-928.65SMESG000018611.1[more]
SMESG000018611.16.676e-724.80SMESG000018611.1[more]
SMESG000080367.13.697e-620.92SMESG000080367.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30029205 ID=SMED30029205|Name=SMED30029205|organism=Schmidtea mediterranea sexual|type=transcript|length=2412bp
ACACGAACTTATTTTTTGATGCCGTTCTTTGTGGAATTTGGGGTCGAATC
CTCTGCCAGAGAATTGGTCTACTGTCTGCCAGTAAAGGTTTACTGTCTAA
TTTTCAAAATGACAATAGAATATTCACATTCTATGTCAATACTAGCGACA
TTTTTGAATTGAAAAACATCGTTTGTCAGGAAAACTTTCAAAGTCTGATA
TTCGATTCCCTTCCGCCGGAATCAGAATCAAAATTGGTTTTTATAACGAG
TTTCTTTCAAATTCCTCTTTTTTGGATCAGTAGCACGTGGCCATGGAAAT
ATACCGCTAAAAATGATAATTCAATCATATTCCAACATAATTTACTTGAC
CTAGCAGAGATCGTGGTAAAATATATCAGAGAATATACAATATCAGAGAC
TTTGCTTGTAGGAGATGAATTTTCTCGTAATTTGTTTTTGTTAGTTTTCA
ACCTGATCAGCCAGACGAGAGTTGAATTGATAACGGGAAAGGAATTAACT
GGAGAAAATTGCTCCTCAAGAATGAGATCCGTTCTGATTCGGATGAAGTG
GAAATTACAAATAATAGTTTTGACAGAACCTGGATCCGTGCCTTTTCTGT
TGAGGAAAAAAAATAGTGCATAATTGCAGTGATGTATTTCTATTGTGATA
TTTGATTGCCGGCCGAATCATGTGGGTACATTCAACCTGATATTACCTGG
ATAATTGTTAATTCATGCCTCCCGCCATTCTCGCTCAGCTTGCCTCTGTA
TTCGTCTATAAACTTCATTTCATTTTTCGATAGCAGATTTTCGAATCAGA
TTTATCCAGACTGCTTCATAAAAAAAGGAGAAGTTGTGATCAAATATATC
ATTGACAATTTGAACTCGCTGACGAACTCAACAGACCTCTTTAACCTAAT
TCAACCTCATAAATGTAATATCGAACGCCCGATAACAAACAAGTCCTTTT
TTACTGAAATTTTCTCGGAATTTAAAAAGTCATTTCTTGCGGGACAAAAT
GGATATCTAAATATTGTAAAAACGCAACTGATTGATCCTAATAACGCAAG
CGTGTCTACGAGTGAATTATACAGAGGTTTCAAATTTGAAAATATTTCCT
TGAGAGTTTGCGTCAGACAGGCTCCAACTCTTTATCACAATTCTACAACT
GGCTTCATTCCGAGGATTTTAACTAAACTCTATGAAAAATTCAATTTCAA
TTGGTCCATCGTTACGCCAGAAACTGGAAATATGGAGAATTGCAATGAAA
AATTGGTTAAGAAACTTGTGGATGTTTCAGTGGATTTTGCTTCCATTTTG
GATACTTCTGACTTGCCTATAGTATTCTGGACGATTCTAGAAGAGGAGCA
TTGTTTTATTCTTGGAGTTGATCCAGTCGATTATGGGGTAACATTGTGGG
CCCCTGTTCAATTTTTTAATTGGACATTTTTATTATTACTCGGAGGATGC
CTTGTAATTTATTCGTTATTGGCATTAATATTCACCAAATTCAATCCACC
GATCGTCGACAGTAATAGCACCATGCCAGCTTTGACTGCAGATTCTTCTT
TTCTTGATTTTTGCTGGAATCTCACCGGGGCTTTTCTTGCACAAGGTTTG
GAATATAATCCGTTGAATCCTAGTCTTAGTACTTTGTCGTTTTTCTGGTG
GGTTTATTCAATAATAATCTCAAGTGTTTATTCCGGAAGCTTGACAGTAA
CAATGACAGTCACAAACAACAGATACAGTTTCCTGTCGAATTCTATATTT
GAGTTGCTTAAAACAGAAAGAGTTGATGTTTTTATTGTCAGAGGAAGTTA
TATTGACAAGTGGCTCAACAATTCAACTGAGAATGTATACAGCCTTCTAA
AATCCAGAAAGATTTACGTTTCAACTCTAGAAGAATGTATCCAAAATGTC
CTCAACCACATCAGTAGCAAAAGTCCCCAACTAGCGGTCTGTCTGGCTCC
ATGCATAAATACGATGACCCCAAGGGAAATCTGTGATAAAATGAGAATGC
TTTCAGACACTCTGTTTCCTATCAGTATTAGTTTAGGCTTTCACGAAATG
TCTCCTTGGAAGAATTTTATTATATCAGAATTGAACACAATGAAGTTCAT
GGGAGTGATGGAACAGTTTCGCCATGACAGCATTTCAAACATCAACTGCT
TGCCTCAAAATCAAAACGACCAATTTTCTGGACAGTCAATCTCAATATCA
GGGCTCATTGGTGTCATTTACTTAATTTACGCCTTAATTTTCATGGGGAT
AATTTGTCTCGGATTAGAAATAATTTATAAAAATTTGAAAAAAAAAGAAT
TGATCAAAAGAAGCATATTTTCAAAACATTTTCAATGATTTTTAGAAACA
TTATTGATTTGTTTGCACATTTAGAAATGAGTTTTATTTTTGGAAGGTAG
AATTAAAAAGGA
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protein sequence of SMED30029205-orf-1

>SMED30029205-orf-1 ID=SMED30029205-orf-1|Name=SMED30029205-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=369bp
MENCNEKLVKKLVDVSVDFASILDTSDLPIVFWTILEEEHCFILGVDPVD
YGVTLWAPVQFFNWTFLLLLGGCLVIYSLLALIFTKFNPPIVDSNSTMPA
LTADSSFLDFCWNLTGAFLAQGLEYNPLNPSLSTLSFFWWVYSIIISSVY
SGSLTVTMTVTNNRYSFLSNSIFELLKTERVDVFIVRGSYIDKWLNNSTE
NVYSLLKSRKIYVSTLEECIQNVLNHISSKSPQLAVCLAPCINTMTPREI
CDKMRMLSDTLFPISISLGFHEMSPWKNFIISELNTMKFMGVMEQFRHDS
ISNINCLPQNQNDQFSGQSISISGLIGVIYLIYALIFMGIICLGLEIIYK
NLKKKELIKRSIFSKHFQ*
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protein sequence of SMED30029205-orf-2

>SMED30029205-orf-2 ID=SMED30029205-orf-2|Name=SMED30029205-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=207bp
TNLFFDAVLCGIWGRILCQRIGLLSASKGLLSNFQNDNRIFTFYVNTSDI
FELKNIVCQENFQSLIFDSLPPESESKLVFITSFFQIPLFWISSTWPWKY
TAKNDNSIIFQHNLLDLAEIVVKYIREYTISETLLVGDEFSRNLFLLVFN
LISQTRVELITGKELTGENCSSRMRSVLIRMKWKLQIIVLTEPGSVPFLL
RKKNSA*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000099neuron
PLANA:0000418head
PLANA:0002032epidermal cell
Vocabulary: molecular function
TermDefinition
GO:0015276ligand-gated ion channel activity
GO:0004970ionotropic glutamate receptor activity
Vocabulary: INTERPRO
TermDefinition
IPR001320Iontro_rcpt
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:3.40.190.10coord: 8..318
e-value: 4.2E-27
score: 96.8
NoneNo IPR availableGENE3DG3DSA:1.10.287.70coord: 50..345
e-value: 4.2E-27
score: 96.8
NoneNo IPR availableGENE3DG3DSA:3.40.190.10coord: 40..266
e-value: 4.2E-27
score: 96.8
NoneNo IPR availablePANTHERPTHR42643:SF13IONOTROPIC RECEPTOR 40A-RELATEDcoord: 73..359
NoneNo IPR availablePANTHERPTHR42643FAMILY NOT NAMEDcoord: 73..359
NoneNo IPR availableTMHMMTMhelixcoord: 61..83
NoneNo IPR availableTMHMMTMhelixcoord: 319..341
NoneNo IPR availableTMHMMTMhelixcoord: 135..157
IPR001320Ionotropic glutamate receptorPFAMPF00060Lig_chancoord: 71..331
e-value: 1.2E-10
score: 41.4