SMED30029134

Overview
NameSMED30029134
Smed IDSMED30029134
Length (bp)425
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30029134 (SMED30029134) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30029134 (SMED30029134) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30029134 (SMED30029134) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30029134 (SMED30029134) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30029134

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
epidermisSMED30029134SMESG000081053.1 SMESG000081051.1 dd_Smed_v4_1691_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30029134SMESG000081053.1 SMESG000081051.1 dd_Smed_v4_1691_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30029134SMESG000081053.1 SMESG000081051.1 dd_Smed_v4_1691_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30029134SMESG000081053.1 SMESG000081051.1 dd_Smed_v6_1691_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30029134 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.000459:153559..158666 -2086
Homology
[Close]
BLAST of SMED30029134 vs. Ensembl Human
Match: DNASE2 (deoxyribonuclease 2, lysosomal [Source:HGNC Symbol;Acc:HGNC:2960])

HSP 1 Score: 77.0258 bits (188), Expect = 6.776e-17
Identity = 39/125 (31.20%), Postives = 68/125 (54.40%), Query Frame = -2 
Query:   59 DIYADFMAPKLKISMDVXX-XXXXXXXXXSCQSKYKVYNVRSISIDGK---KYKSTQDHCKWAVSTSIEYPFVCIGDVNRMYSQFKRGGGSLCVKNDKIWSLFQKIVREIEPCPKLQKDIREIFK 421
            D+Y+ ++A  L  ++ V+        + S+C   ++V NV  I+  G     + ST+DH KW VS   + P+ C+GD+NR   + +RGGG+LC +   +W  FQ +V+  +PC  + +     +K
Sbjct:  237 DLYSGWLAAALGTNLQVQFWHKTVGILPSNCSDIWQVLNVNQIAFPGPAGPSFNSTEDHSKWCVSP--KGPWTCVGDMNRNQGEEQRGGGTLCAQLPALWKAFQPLVKNYQPCNGMARKPSRAYK 359
[Close]
BLAST of SMED30029134 vs. Ensembl Human
Match: DNASE2B (deoxyribonuclease 2 beta [Source:HGNC Symbol;Acc:HGNC:28875])

HSP 1 Score: 66.6254 bits (161), Expect = 3.326e-14
Identity = 41/112 (36.61%), Postives = 63/112 (56.25%), Query Frame = -2 
Query:   95 DIYADFMAPKLKISMDVXX-XXXXXXXXXSCQSKYKVYNVRSISIDGKKYKST-QDHCKWAVST-SIEYPFVCIGDVNRMYSQFKRGGGSLCVKNDKIWSLFQKIVREIEPC 421
            DI+A +MA +LK  +  E+   +   + S+C   Y VYN+++I +    Y S+ QDH KW +S    +  + CIGD+NR   Q  R GG +C +N +I+  FQ +V   E C
Sbjct:   41 DIFAAWMAQRLKTHLLTETWQRKRQELPSNCSLPYHVYNIKAIKLSRHSYFSSYQDHAKWCISQKGTKNRWTCIGDLNRSPHQAFRSGGFICTQNWQIYQAFQGLVLYYESC 152
[Close]
BLAST of SMED30029134 vs. Ensembl Human
Match: DNASE2B (deoxyribonuclease 2 beta [Source:HGNC Symbol;Acc:HGNC:28875])

HSP 1 Score: 66.2402 bits (160), Expect = 3.889e-13
Identity = 41/112 (36.61%), Postives = 63/112 (56.25%), Query Frame = -2 
Query:   95 DIYADFMAPKLKISMDVXX-XXXXXXXXXSCQSKYKVYNVRSISIDGKKYKST-QDHCKWAVST-SIEYPFVCIGDVNRMYSQFKRGGGSLCVKNDKIWSLFQKIVREIEPC 421
            DI+A +MA +LK  +  E+   +   + S+C   Y VYN+++I +    Y S+ QDH KW +S    +  + CIGD+NR   Q  R GG +C +N +I+  FQ +V   E C
Sbjct:  249 DIFAAWMAQRLKTHLLTETWQRKRQELPSNCSLPYHVYNIKAIKLSRHSYFSSYQDHAKWCISQKGTKNRWTCIGDLNRSPHQAFRSGGFICTQNWQIYQAFQGLVLYYESC 360
[Close]
BLAST of SMED30029134 vs. Ensembl Celegans
Match: crn-7 (Cell-death-related nuclease 7 [Source:UniProtKB/Swiss-Prot;Acc:P34387])

HSP 1 Score: 92.0485 bits (227), Expect = 7.673e-23
Identity = 44/111 (39.64%), Postives = 69/111 (62.16%), Query Frame = -2 
Query:   95 DIYADFMAPKLKISMDVXX--XXXXXXXXXSCQSKYKVYNVRSISIDGKKYKSTQDHCKWAVSTSIEYPFVCIGDVNRMYSQFKRGGGSLCVKNDKIWSLFQKIVREIEPC 421
            DI+ D ++ + K+++ VES        I ++C S  + ++V  + + G  + S++DH KWAVS S   P VC GD+NR  SQ KRGGG+LC++N  +W L+   V ++EPC
Sbjct:  232 DIWNDLISRQNKVTLAVESWLNGSGDDIHTTCTSTSQTHDVTEMRVTGLNFASSKDHSKWAVSNSQTNPIVCFGDMNRQKSQLKRGGGALCIQNRNLWQLYHSFVIQVEPC 342
[Close]
BLAST of SMED30029134 vs. Ensembl Celegans
Match: nuc-1 (Deoxyribonuclease-2 [Source:UniProtKB/Swiss-Prot;Acc:Q17778])

HSP 1 Score: 69.707 bits (169), Expect = 9.404e-15
Identity = 40/106 (37.74%), Postives = 59/106 (55.66%), Query Frame = -2 
Query:  107 DIYADFMAPKLKISMDVXXXXXXXXXXXSCQSKYKVYNVRSISIDGKK-YKSTQDHCKWAVSTSIEYPFVCIGDVNRMYSQFKRGGGSLCVKNDKIWSLFQKIVRE 421
            D+Y   +AP LK  + VE+W R S I   C+  Y   +  SI +     +  T+DH K A S  +  P+VCIGD+NRM SQ+ RGGG+ C+ +  +W  +  I  +
Sbjct:  266 DLYDTLVAPTLKTDLVVETWRRGSEIPLDCKLTYHANDALSIHVGSTTAFSYTKDHSKMAHSADMTKPWVCIGDINRMTSQYVRGGGTTCISSSFLWKAYSVIATQ 371
[Close]
BLAST of SMED30029134 vs. Ensembl Celegans
Match: crn-6 (Cell death-related nuclease 6 [Source:UniProtKB/Swiss-Prot;Acc:P34508])

HSP 1 Score: 64.3142 bits (155), Expect = 8.694e-13
Identity = 45/126 (35.71%), Postives = 66/126 (52.38%), Query Frame = -2 
Query:   89 DIYADFMAPKLKISMDVXXXXXXXXXXXSCQSKYKVYNVRSI----------SIDGKKYKSTQDHCKWAV-----STSIEYPFVCIGDVNRMYSQFKRGGGSLCVKNDKIWSLFQKIVREIEPCPK 421
            D+Y+DF+ P LK S+ VE+W   S  E +  S     +V+S           +ID   Y S +DH K+ V     S+    P+VCIGD+NR   Q  RGGG++C+ + + +  F  I+ E  PC K
Sbjct:  236 DLYSDFVGPTLKSSIKVETWNHQSGDEYNLPSVCDPNHVQSTMSAKYIRLPYAID---YSSYEDHSKFVVAYSESSSKPPIPYVCIGDINRQSHQIHRGGGTMCIYDQETYFQFANIISETVPCTK 358
[Close]
BLAST of SMED30029134 vs. Ensembl Celegans
Match: crn-6 (Cell death-related nuclease 6 [Source:UniProtKB/Swiss-Prot;Acc:P34508])

HSP 1 Score: 64.3142 bits (155), Expect = 8.694e-13
Identity = 45/126 (35.71%), Postives = 66/126 (52.38%), Query Frame = -2 
Query:   89 DIYADFMAPKLKISMDVXXXXXXXXXXXSCQSKYKVYNVRSI----------SIDGKKYKSTQDHCKWAV-----STSIEYPFVCIGDVNRMYSQFKRGGGSLCVKNDKIWSLFQKIVREIEPCPK 421
            D+Y+DF+ P LK S+ VE+W   S  E +  S     +V+S           +ID   Y S +DH K+ V     S+    P+VCIGD+NR   Q  RGGG++C+ + + +  F  I+ E  PC K
Sbjct:  236 DLYSDFVGPTLKSSIKVETWNHQSGDEYNLPSVCDPNHVQSTMSAKYIRLPYAID---YSSYEDHSKFVVAYSESSSKPPIPYVCIGDINRQSHQIHRGGGTMCIYDQETYFQFANIISETVPCTK 358
[Close]
BLAST of SMED30029134 vs. Ensembl Celegans
Match: crn-6 (Cell death-related nuclease 6 [Source:UniProtKB/Swiss-Prot;Acc:P34508])

HSP 1 Score: 64.3142 bits (155), Expect = 8.861e-13
Identity = 45/126 (35.71%), Postives = 66/126 (52.38%), Query Frame = -2 
Query:   89 DIYADFMAPKLKISMDVXXXXXXXXXXXSCQSKYKVYNVRSI----------SIDGKKYKSTQDHCKWAV-----STSIEYPFVCIGDVNRMYSQFKRGGGSLCVKNDKIWSLFQKIVREIEPCPK 421
            D+Y+DF+ P LK S+ VE+W   S  E +  S     +V+S           +ID   Y S +DH K+ V     S+    P+VCIGD+NR   Q  RGGG++C+ + + +  F  I+ E  PC K
Sbjct:  236 DLYSDFVGPTLKSSIKVETWNHQSGDEYNLPSVCDPNHVQSTMSAKYIRLPYAID---YSSYEDHSKFVVAYSESSSKPPIPYVCIGDINRQSHQIHRGGGTMCIYDQETYFQFANIISETVPCTK 358
[Close]
BLAST of SMED30029134 vs. Ensembl Fly
Match: DNaseII (gene:FBgn0000477 transcript:FBtr0083538)

HSP 1 Score: 70.4774 bits (171), Expect = 6.486e-15
Identity = 43/125 (34.40%), Postives = 71/125 (56.80%), Query Frame = -2 
Query:   89 LDIYADFMAPKLKISMDVXX-XXXXXXXXXSCQSKYKVYNVRSISID--GKKYKSTQDHCKWAVSTSIEY----------PFVCIGDVNRMYSQFKRGGGSLCVKNDKIWSLFQKIVREIEPCPK 424
            +++YAD +AP L +S+ VE+    +  + +SC    KV NV SIS       +K+TQDH KWAVS                ++C+GD+NR   Q  RGGG++C K+ ++ +L++++V   + C +
Sbjct:  240 VELYADVVAPTLDVSLFVEAWRDGAGNLPNSCDKSDKVLNVESISNPELSVDFKTTQDHSKWAVSRPTGILIYHWRVGGGDWICVGDINRQEGQLHRGGGTVCHKSARVSNLYRQLVTNYDKCAQ 364
[Close]
BLAST of SMED30029134 vs. Ensembl Zebrafish
Match: dnase2 (deoxyribonuclease II, lysosomal [Source:ZFIN;Acc:ZDB-GENE-010724-16])

HSP 1 Score: 63.929 bits (154), Expect = 1.777e-12
Identity = 38/113 (33.63%), Postives = 67/113 (59.29%), Query Frame = -2 
Query:   95 DIYADFMAPKLKISMDVXX-XXXXXXXXXSCQSKYKVYNVRSISIDGK-KYKSTQDHCKWAVSTSIEYP--FVCIGDVNRMYSQFKRGGGSLCVKNDKIWSLFQKIVREIEPC 421
            D+Y  ++AP L+ ++ V+     +  + S C   ++V N+  IS   K  +K+T+DH KWAVS+S      +VC+GD+NR  ++ KRGGG++C ++  +W  ++    + E C
Sbjct:  241 DLYHSWVAPTLQSNLLVQFWRRSTGILPSDCSPNWEVLNIDLISPGQKVTFKATEDHSKWAVSSSSGTSGGWVCVGDINRDEAEEKRGGGTVCRRDAVVWKAYRSAALQCESC 353
[Close]
BLAST of SMED30029134 vs. Ensembl Zebrafish
Match: dnase2 (deoxyribonuclease II, lysosomal [Source:ZFIN;Acc:ZDB-GENE-010724-16])

HSP 1 Score: 63.929 bits (154), Expect = 1.777e-12
Identity = 38/113 (33.63%), Postives = 67/113 (59.29%), Query Frame = -2 
Query:   95 DIYADFMAPKLKISMDVXX-XXXXXXXXXSCQSKYKVYNVRSISIDGK-KYKSTQDHCKWAVSTSIEYP--FVCIGDVNRMYSQFKRGGGSLCVKNDKIWSLFQKIVREIEPC 421
            D+Y  ++AP L+ ++ V+     +  + S C   ++V N+  IS   K  +K+T+DH KWAVS+S      +VC+GD+NR  ++ KRGGG++C ++  +W  ++    + E C
Sbjct:  241 DLYHSWVAPTLQSNLLVQFWRRSTGILPSDCSPNWEVLNIDLISPGQKVTFKATEDHSKWAVSSSSGTSGGWVCVGDINRDEAEEKRGGGTVCRRDAVVWKAYRSAALQCESC 353
[Close]
BLAST of SMED30029134 vs. Ensembl Zebrafish
Match: dnase2b (deoxyribonuclease II beta [Source:ZFIN;Acc:ZDB-GENE-090824-2])

HSP 1 Score: 46.595 bits (109), Expect = 2.028e-6
Identity = 36/111 (32.43%), Postives = 63/111 (56.76%), Query Frame = -2 
Query:   95 DIYADFMAPKLKISMDVXX-XXXXXXXXXSCQSKYKVYNVRSISIDGKK-YKSTQDHCKWAVSTSIEYPFVCIGDVNRMYSQFKRGGGSLCVKNDKIWSLFQKIVREIEPC 421
            DIY  ++A  L   + VES   ++ ++ S+C   + V N+ ++ + G + ++S +DH KW VS + +  + C+GD+NR   Q  RGGG  C +N  I+  F+K +   + C
Sbjct:  238 DIYTGWVAQALGTDLLVESWLHQAHQLPSNCSLPWHVMNINAVCLPGPQMFRSYEDHSKWCVSYTFKDQWTCLGDLNRDGGQAGRGGGLTCCQNSVIYKAFRKAMARYKHC 348
[Close]
BLAST of SMED30029134 vs. Ensembl Zebrafish
Match: dnase2b (deoxyribonuclease II beta [Source:ZFIN;Acc:ZDB-GENE-090824-2])

HSP 1 Score: 46.595 bits (109), Expect = 2.148e-6
Identity = 36/111 (32.43%), Postives = 63/111 (56.76%), Query Frame = -2 
Query:   95 DIYADFMAPKLKISMDVXX-XXXXXXXXXSCQSKYKVYNVRSISIDGKK-YKSTQDHCKWAVSTSIEYPFVCIGDVNRMYSQFKRGGGSLCVKNDKIWSLFQKIVREIEPC 421
            DIY  ++A  L   + VES   ++ ++ S+C   + V N+ ++ + G + ++S +DH KW VS + +  + C+GD+NR   Q  RGGG  C +N  I+  F+K +   + C
Sbjct:  227 DIYTGWVAQALGTDLLVESWLHQAHQLPSNCSLPWHVMNINAVCLPGPQMFRSYEDHSKWCVSYTFKDQWTCLGDLNRDGGQAGRGGGLTCCQNSVIYKAFRKAMARYKHC 337
[Close]
BLAST of SMED30029134 vs. Ensembl Xenopus
Match: dnase2b (deoxyribonuclease II beta [Source:NCBI gene;Acc:496759])

HSP 1 Score: 74.3294 bits (181), Expect = 4.164e-16
Identity = 43/117 (36.75%), Postives = 70/117 (59.83%), Query Frame = -2 
Query:   74 DIYADFMAPKLKISMDVXXXXXXXXXXXSCQSKYKVYNVRSISIDGKK-YKSTQDHCKWAVSTSIEYPFVCIGDVNRMYSQFKRGGGSLCVKNDKIWSLFQKIVREIEPCPKLQKDI 421
            DI+A ++A KL+ +M VESW    ++ S+C  +Y VYN+  I +  +  +++  DH KW VS S E  + CIGD+NR  +Q +R GG +C +N  I++ F+ +V   + C   +  I
Sbjct:  224 DIFAAWIAQKLRTNMLVESWQSRQQLSSNCSLQYHVYNINRIGLPTQSTFQTFYDHSKWCVSQSNEDLWTCIGDLNRHPAQTRRSGGFICTQNKHIYTAFRNMVLYYKNCTDTEYKI 340
[Close]
BLAST of SMED30029134 vs. Ensembl Xenopus
Match: dnase2 (deoxyribonuclease II, lysosomal [Source:NCBI gene;Acc:733868])

HSP 1 Score: 56.225 bits (134), Expect = 1.510e-9
Identity = 25/81 (30.86%), Postives = 41/81 (50.62%), Query Frame = -2 
Query:   95 CQSKYKVYNVRSISID-GKKYKSTQDHCKWAVSTSIEYPFVCIGDVNRMYSQFKRGGGSLCVKNDKIWSLFQKIVREIEPC 334
            C   +  YN+  I I     + +  DH KW V+    +   C+GD+NR   + +RGGG++CV +  +W  F+ +V     C
Sbjct:  265 CSLPFHTYNIMEIDISCAYSFSTHNDHSKWCVTDGAGW--ACVGDMNRDVKEERRGGGTVCVNDPNVWKSFRSLVSSYNNC 343
[Close]
BLAST of SMED30029134 vs. Ensembl Mouse
Match: Dnase2a (deoxyribonuclease II alpha [Source:MGI Symbol;Acc:MGI:1329019])

HSP 1 Score: 70.0922 bits (170), Expect = 1.277e-14
Identity = 34/113 (30.09%), Postives = 62/113 (54.87%), Query Frame = -2 
Query:   95 DIYADFMAPKLKISMDVXX-XXXXXXXXXSCQSKYKVYNVRSISIDGKK---YKSTQDHCKWAVSTSIEYPFVCIGDVNRMYSQFKRGGGSLCVKNDKIWSLFQKIVREIEPC 421
            D+Y+ ++A  L  ++ V+        + S+C   Y+V +V      G     + +T+DH KW V+   + P+ C+GD+NR  ++  RGGG++C +    W  FQ +V++ +PC
Sbjct:  218 DLYSGWLAEALGTNLQVQFWQNSPGILPSNCSGAYQVLDVTQTGFPGPSRLTFSATEDHSKWCVAP--QGPWACVGDMNRNKAETHRGGGTVCTQLPSFWKAFQSLVKDWKPC 328
[Close]
BLAST of SMED30029134 vs. Ensembl Mouse
Match: Dnase2a (deoxyribonuclease II alpha [Source:MGI Symbol;Acc:MGI:1329019])

HSP 1 Score: 69.707 bits (169), Expect = 1.539e-14
Identity = 34/113 (30.09%), Postives = 62/113 (54.87%), Query Frame = -2 
Query:   95 DIYADFMAPKLKISMDVXX-XXXXXXXXXSCQSKYKVYNVRSISIDGKK---YKSTQDHCKWAVSTSIEYPFVCIGDVNRMYSQFKRGGGSLCVKNDKIWSLFQKIVREIEPC 421
            D+Y+ ++A  L  ++ V+        + S+C   Y+V +V      G     + +T+DH KW V+   + P+ C+GD+NR  ++  RGGG++C +    W  FQ +V++ +PC
Sbjct:  239 DLYSGWLAEALGTNLQVQFWQNSPGILPSNCSGAYQVLDVTQTGFPGPSRLTFSATEDHSKWCVAP--QGPWACVGDMNRNKAETHRGGGTVCTQLPSFWKAFQSLVKDWKPC 349
[Close]
BLAST of SMED30029134 vs. Ensembl Mouse
Match: Dnase2b (deoxyribonuclease II beta [Source:MGI Symbol;Acc:MGI:1913283])

HSP 1 Score: 67.3958 bits (163), Expect = 1.327e-13
Identity = 41/105 (39.05%), Postives = 58/105 (55.24%), Query Frame = -2 
Query:  116 DIYADFMAPKLKISMDVXX-XXXXXXXXXSCQSKYKVYNVRSISIDGKKY-KSTQDHCKWAVST-SIEYPFVCIGDVNRMYSQFKRGGGSLCVKNDKIWSLFQKI 421
            DI+  ++A KLK  +  ++   +   + S+C   Y VYN++SI +  K Y  S QDH KW VS       + CIGD+NR   Q  RGGG +C KN  I+  F K+
Sbjct:  242 DIFTGWIAQKLKTHLLAQTWQKKKQELPSNCSLPYHVYNIKSIGVTSKSYFSSRQDHSKWCVSIKGSANRWTCIGDLNRSLHQALRGGGFICTKNHYIYQAFHKL 346
[Close]
BLAST of SMED30029134 vs. Ensembl Mouse
Match: Dnase2b (deoxyribonuclease II beta [Source:MGI Symbol;Acc:MGI:1913283])

HSP 1 Score: 67.3958 bits (163), Expect = 1.327e-13
Identity = 41/105 (39.05%), Postives = 58/105 (55.24%), Query Frame = -2 
Query:  116 DIYADFMAPKLKISMDVXX-XXXXXXXXXSCQSKYKVYNVRSISIDGKKY-KSTQDHCKWAVST-SIEYPFVCIGDVNRMYSQFKRGGGSLCVKNDKIWSLFQKI 421
            DI+  ++A KLK  +  ++   +   + S+C   Y VYN++SI +  K Y  S QDH KW VS       + CIGD+NR   Q  RGGG +C KN  I+  F K+
Sbjct:  242 DIFTGWIAQKLKTHLLAQTWQKKKQELPSNCSLPYHVYNIKSIGVTSKSYFSSRQDHSKWCVSIKGSANRWTCIGDLNRSLHQALRGGGFICTKNHYIYQAFHKL 346
[Close]
BLAST of SMED30029134 vs. UniProt/SwissProt
Match: sp|P34387|TG198_CAEEL (Cell-death-related nuclease 7 OS=Caenorhabditis elegans OX=6239 GN=crn-7 PE=2 SV=2)

HSP 1 Score: 92.0485 bits (227), Expect = 9.761e-22
Identity = 44/111 (39.64%), Postives = 69/111 (62.16%), Query Frame = -2 
Query:   95 DIYADFMAPKLKISMDVXX--XXXXXXXXXSCQSKYKVYNVRSISIDGKKYKSTQDHCKWAVSTSIEYPFVCIGDVNRMYSQFKRGGGSLCVKNDKIWSLFQKIVREIEPC 421
            DI+ D ++ + K+++ VES        I ++C S  + ++V  + + G  + S++DH KWAVS S   P VC GD+NR  SQ KRGGG+LC++N  +W L+   V ++EPC
Sbjct:  232 DIWNDLISRQNKVTLAVESWLNGSGDDIHTTCTSTSQTHDVTEMRVTGLNFASSKDHSKWAVSNSQTNPIVCFGDMNRQKSQLKRGGGALCIQNRNLWQLYHSFVIQVEPC 342
[Close]
BLAST of SMED30029134 vs. UniProt/SwissProt
Match: sp|Q75WF2|DNA2_ACAPL (Plancitoxin-1 OS=Acanthaster planci OX=133434 PE=1 SV=1)

HSP 1 Score: 78.1814 bits (191), Expect = 1.294e-16
Identity = 35/81 (43.21%), Postives = 50/81 (61.73%), Query Frame = -2 
Query:   95 CQSKYKVYNVRSISIDG-KKYKSTQDHCKWAVSTSIEYPFVCIGDVNRMYSQFKRGGGSLCVKNDKIWSLFQKIVREIEPC 334
            C++   VYNV+ +S+ G   +K T+DH KWAV+T     + CIG +NR  SQ  RGGG++C++N  +   F   V E EPC
Sbjct:  277 CEAGLNVYNVKKVSLSGGSDFKGTKDHSKWAVTTKSGLKWTCIGGINRQTSQMYRGGGAVCLENANVHKAFYDSVAEYEPC 357
[Close]
BLAST of SMED30029134 vs. UniProt/SwissProt
Match: sp|O00115|DNS2A_HUMAN (Deoxyribonuclease-2-alpha OS=Homo sapiens OX=9606 GN=DNASE2 PE=1 SV=2)

HSP 1 Score: 77.0258 bits (188), Expect = 3.254e-16
Identity = 39/125 (31.20%), Postives = 68/125 (54.40%), Query Frame = -2 
Query:   59 DIYADFMAPKLKISMDVXX-XXXXXXXXXSCQSKYKVYNVRSISIDGK---KYKSTQDHCKWAVSTSIEYPFVCIGDVNRMYSQFKRGGGSLCVKNDKIWSLFQKIVREIEPCPKLQKDIREIFK 421
            D+Y+ ++A  L  ++ V+        + S+C   ++V NV  I+  G     + ST+DH KW VS   + P+ C+GD+NR   + +RGGG+LC +   +W  FQ +V+  +PC  + +     +K
Sbjct:  237 DLYSGWLAAALGTNLQVQFWHKTVGILPSNCSDIWQVLNVNQIAFPGPAGPSFNSTEDHSKWCVSP--KGPWTCVGDMNRNQGEEQRGGGTLCAQLPALWKAFQPLVKNYQPCNGMARKPSRAYK 359
[Close]
BLAST of SMED30029134 vs. UniProt/SwissProt
Match: sp|Q9QZK8|DNS2A_RAT (Deoxyribonuclease-2-alpha OS=Rattus norvegicus OX=10116 GN=Dnase2 PE=2 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 5.585e-14
Identity = 35/113 (30.97%), Postives = 62/113 (54.87%), Query Frame = -2 
Query:   95 DIYADFMAPKLKISMDVXX-XXXXXXXXXSCQSKYKVYNVRSISI---DGKKYKSTQDHCKWAVSTSIEYPFVCIGDVNRMYSQFKRGGGSLCVKNDKIWSLFQKIVREIEPC 421
            D+Y+ ++A  L  ++ V+        + S+C   +K+ +V         G  + +T+DH KW V+   E P+VC+GD+NR   +  RGGG+LC +   +W  F+ +V+  +PC
Sbjct:  240 DLYSGWLAAALGTNLQVQFWPNSPGILPSNCSGTHKILDVTETGFPGPSGPTFNATEDHSKWCVAP--EGPWVCVGDMNRNKRETHRGGGTLCTQVPALWKAFRSLVKACKPC 350
[Close]
BLAST of SMED30029134 vs. UniProt/SwissProt
Match: sp|P56542|DNS2A_MOUSE (Deoxyribonuclease-2-alpha OS=Mus musculus OX=10090 GN=Dnase2 PE=1 SV=1)

HSP 1 Score: 69.707 bits (169), Expect = 1.076e-13
Identity = 34/113 (30.09%), Postives = 62/113 (54.87%), Query Frame = -2 
Query:   95 DIYADFMAPKLKISMDVXX-XXXXXXXXXSCQSKYKVYNVRSISIDGKK---YKSTQDHCKWAVSTSIEYPFVCIGDVNRMYSQFKRGGGSLCVKNDKIWSLFQKIVREIEPC 421
            D+Y+ ++A  L  ++ V+        + S+C   Y+V +V      G     + +T+DH KW V+   + P+ C+GD+NR  ++  RGGG++C +    W  FQ +V++ +PC
Sbjct:  239 DLYSGWLAEALGTNLQVQFWQNSPGILPSNCSGAYQVLDVTQTGFPGPSRLTFSATEDHSKWCVAP--QGPWACVGDMNRNKAETHRGGGTVCTQLPSFWKAFQSLVKDWKPC 349
[Close]
BLAST of SMED30029134 vs. TrEMBL
Match: A0A0B2W4P7 (Cell-death-related nuclease 7 OS=Toxocara canis OX=6265 GN=crn-7 PE=4 SV=1)

HSP 1 Score: 114.005 bits (284), Expect = 3.453e-27
Identity = 55/110 (50.00%), Postives = 77/110 (70.00%), Query Frame = -2 
Query:   95 DIYADFMAPKLKISMDVXX-XXXXXXXXXSCQSKYKVYNVRSISIDGKKYKSTQDHCKWAVSTSIEYPFVCIGDVNRMYSQFKRGGGSLCVKNDKIWSLFQKIVREIEPC 421
            DIY++ +APKL+ S+  E+       + SSC S YKV N+ S+ IDGK++KS++DH KWAVS     P +CIGD+NR  SQ KRGGG++C++N ++W L+   V EIEPC
Sbjct:  260 DIYSELLAPKLRTSLLTETWLNGPGDLPSSCSSTYKVNNIVSVLIDGKQFKSSKDHSKWAVSADKRNPMLCIGDINRQKSQSKRGGGTVCIRNAQLWHLYNGSVDEIEPC 369
[Close]
BLAST of SMED30029134 vs. TrEMBL
Match: A0A0M3KCV0 (Cell-death-related nuclease 7 (inferred by orthology to a C. elegans protein) OS=Anisakis simplex OX=6269 PE=4 SV=1)

HSP 1 Score: 103.219 bits (256), Expect = 7.659e-26
Identity = 44/105 (41.90%), Postives = 73/105 (69.52%), Query Frame = -2 
Query:   95 FMAPKLKISMDVXX-XXXXXXXXXSCQSKYKVYNVRSISIDGKKYKSTQDHCKWAVSTSIEYPFVCIGDVNRMYSQFKRGGGSLCVKNDKIWSLFQKIVREIEPC 406
             +AP++K S+  E+     +  +S C SKY V N+ S+++DG ++K+++DH KWA+S   + P +CIGD+NR  SQ KRGGG++C+ ND++W L+ K +  ++PC
Sbjct:    1 LVAPEMKTSLFTETWMNGPNDFDSECSSKYTVNNIESLNVDGNQFKNSKDHSKWAISVDEKEPLLCIGDINRQKSQMKRGGGTVCILNDRLWGLYNKTIVNVQPC 105
[Close]
BLAST of SMED30029134 vs. TrEMBL
Match: A0A3P6TGL9 (Uncharacterized protein OS=Anisakis simplex OX=6269 GN=ASIM_LOCUS18198 PE=4 SV=1)

HSP 1 Score: 103.219 bits (256), Expect = 9.420e-26
Identity = 44/105 (41.90%), Postives = 73/105 (69.52%), Query Frame = -2 
Query:   95 FMAPKLKISMDVXX-XXXXXXXXXSCQSKYKVYNVRSISIDGKKYKSTQDHCKWAVSTSIEYPFVCIGDVNRMYSQFKRGGGSLCVKNDKIWSLFQKIVREIEPC 406
             +AP++K S+  E+     +  +S C SKY V N+ S+++DG ++K+++DH KWA+S   + P +CIGD+NR  SQ KRGGG++C+ ND++W L+ K +  ++PC
Sbjct:    1 MVAPEMKTSLFTETWMNGPNDFDSECSSKYTVNNIESLNVDGNQFKNSKDHSKWAISVDEKEPLLCIGDINRQKSQMKRGGGTVCILNDRLWGLYNKTIVNVQPC 105
[Close]
BLAST of SMED30029134 vs. TrEMBL
Match: F1L824 (Cell-death-related nuclease 7 OS=Ascaris suum OX=6253 PE=2 SV=1)

HSP 1 Score: 109.383 bits (272), Expect = 1.424e-25
Identity = 53/133 (39.85%), Postives = 84/133 (63.16%), Query Frame = -2 
Query:   56 DIYADFMAPKLKISMDVXX-XXXXXXXXXSCQSKYKVYNVRSISIDGKKYKSTQDHCKWAVSTSIEYPFVCIGDVNRMYSQFKRGGGSLCVKNDKIWSLFQKIVREIEPC----------PKLQKDIREIFKD 421
            D+Y+D +AP+LK S+  E+       + S C S YKV NV S+ +DG ++ +++DH KWAVS     P +CIGD+NR  SQ+ RGGG++C++N ++WSL++  + E EPC           +L K I+ +F++
Sbjct:  239 DLYSDLVAPQLKTSLLTETWLNGPGDLPSICSSPYKVNNVLSVRVDGNQFANSKDHSKWAVSADGTDPVLCIGDINRQKSQWGRGGGTVCIRNTQVWSLYKGSITETEPCDGVTNHPNWSTRLWKRIKGVFQN 371
[Close]
BLAST of SMED30029134 vs. TrEMBL
Match: A0A443S164 (Plancitoxin-1-like protein (Fragment) OS=Leptotrombidium deliense OX=299467 GN=B4U80_02519 PE=4 SV=1)

HSP 1 Score: 101.679 bits (252), Expect = 3.058e-24
Identity = 51/121 (42.15%), Postives = 73/121 (60.33%), Query Frame = -2 
Query:   89 DIYADFMAPKLK--ISMDVXXXXXXXXXXXSCQSKYKVYNVRSISIDGK--------KYKSTQDHCKWAVSTSIEYPFVCIGDVNRMYSQFKRGGGSLCVKNDKIWSLFQKIVREIEPCPK 421
            D+Y  ++AP LK  +S++         + S+C S YK+ NV ++ +  K        ++  TQDH KWAV+T+   PFVCI D+NRM SQFKRGGGSLC  +  +W  F  I++ +E CPK
Sbjct:   72 DLYGAYIAPSLKNDLSVETWRRGAGGPLTSNCSSTYKIINVDTLEMKFKGGNIFTTDEWLYTQDHAKWAVTTASISPFVCIADINRMESQFKRGGGSLCFNDSDVWKQFNDIIKGVEACPK 192
[Close]
BLAST of SMED30029134 vs. Ensembl Cavefish
Match: dnase2 (deoxyribonuclease-2-alpha-like [Source:NCBI gene;Acc:103028211])

HSP 1 Score: 73.9442 bits (180), Expect = 4.572e-16
Identity = 41/111 (36.94%), Postives = 67/111 (60.36%), Query Frame = -2 
Query:   95 DIYADFMAPKLKISMDVXX-XXXXXXXXXSCQSKYKVYNVRSISIDGK-KYKSTQDHCKWAVSTSIEYPFVCIGDVNRMYSQFKRGGGSLCVKNDKIWSLFQKIVREIEPC 421
            D+Y  ++AP L+  + V+     +  +ES C   YKV N++ +S   K  +KST+DH KWA S S ++  VC+GD+NR  ++ KRGGG++C KN  +W  ++    + + C
Sbjct:  247 DLYHSWVAPALQADLLVQFWRRSTGILESDCDPSYKVLNIQLLSPGQKLTFKSTEDHSKWAASVSGDW--VCVGDINRNEAEEKRGGGTVCHKNAVVWKAYRATALQCDSC 355
[Close]
BLAST of SMED30029134 vs. Ensembl Cavefish
Match: dnase2b (deoxyribonuclease 2 beta [Source:NCBI gene;Acc:103030372])

HSP 1 Score: 61.6178 bits (148), Expect = 1.023e-11
Identity = 36/111 (32.43%), Postives = 62/111 (55.86%), Query Frame = -2 
Query:   95 DIYADFMAPKLKISMDVXX-XXXXXXXXXSCQSKYKVYNVRSISI-DGKKYKSTQDHCKWAVSTSIEYPFVCIGDVNRMYSQFKRGGGSLCVKNDKIWSLFQKIVREIEPC 421
            DIYA ++A  L+  + VES    + ++ S+C   Y V N++ + + +   + S +DH KW VS      + C+GD+NR  +Q  RGGG +C +N  ++  F++ V   + C
Sbjct:  255 DIYAGWVAQTLRTDLLVESWQRDNHQLPSNCSLPYHVLNIKRVHLPENITFSSYEDHSKWCVSVEFRAQWTCLGDLNRESTQAYRGGGLICSQNPVVYKAFRQAVAWFKGC 365
[Close]
BLAST of SMED30029134 vs. Ensembl Sea Lamprey
Match: dnase2 (deoxyribonuclease II, lysosomal [Source:ZFIN;Acc:ZDB-GENE-010724-16])

HSP 1 Score: 75.0998 bits (183), Expect = 3.232e-17
Identity = 43/120 (35.83%), Postives = 65/120 (54.17%), Query Frame = -2 
Query:   77 DIYADFMAPKLKISMDVXX-XXXXXXXXXSCQSKYKVYNVRSISIDG-KKYKSTQDHCKWAVSTSIEYP---FVCIGDVNRMYSQFKRGGGSLCVKNDKIWSLFQKIVREIEPCPKLQKD 421
            D+Y   +AP LK S+ +ES        +S+C  ++ V  V SIS+     + S +DH KW VS +   P   + C+GD+NR   +  RGGG++C  +  IW+ F K    +EPCP   +D
Sbjct:  214 DLYVHLVAPNLKSSLYLESWVHGVGVFDSNCSGEFCVCRVSSISLPSVAPFSSEEDHSKWCVSQTGGSPGGAWACVGDINRNRGEEYRGGGTVCTDDPAIWAAFAKATTSVEPCPAGPRD 333
[Close]
BLAST of SMED30029134 vs. Ensembl Medaka
Match: ENSORLT00000036979.1 (deoxyribonuclease-2-alpha [Source:NCBI gene;Acc:101173852])

HSP 1 Score: 62.3882 bits (150), Expect = 5.500e-12
Identity = 27/79 (34.18%), Postives = 46/79 (58.23%), Query Frame = -2 
Query:   86 YKVYNVRSISIDGKKYKSTQDHCKWAVSTSIEYPFVCIGDVNRMYSQFKRGGGSLCVKNDKIWSLFQKIVREIEPCPKL 322
            + VYNV+++ +  + +  T DH KW V++   +   C+ D+NR  SQ  RGGG+LC+ + ++   F+  +   EPC  L
Sbjct:  271 HHVYNVKTVKLLKRDFNDTVDHSKWCVTSDENW--TCVADMNRETSQMGRGGGALCMDSYEVAKAFRSTISAYEPCQTL 347
[Close]
BLAST of SMED30029134 vs. Ensembl Medaka
Match: dnase2 (deoxyribonuclease 2, lysosomal [Source:NCBI gene;Acc:101162954])

HSP 1 Score: 61.2326 bits (147), Expect = 1.253e-11
Identity = 35/116 (30.17%), Postives = 65/116 (56.03%), Query Frame = -2 
Query:   95 DIYADFMAPKLKISMDVXX-XXXXXXXXXSCQSKYKVYNVRSISIDGK-KYKSTQDHCKWAVSTSIEYP-----FVCIGDVNRMYSQFKRGGGSLCVKNDKIWSLFQKIVREIEPC 421
            D+Y  ++AP L+  + V+     +  + S C   +KV ++  I+      +K++QDH KWAVS    +      +VC+GD+NR  ++ KRGGG++C+++  +W  ++    + E C
Sbjct:  246 DLYHGWVAPALQSDLLVQFWIRSTGILPSDCSLGWKVLDITLINPGQTFAFKNSQDHSKWAVSPKQAWGGKGGGWVCVGDINRNEAEEKRGGGTVCLQDPVVWKAYRSAALQCEAC 361
[Close]
BLAST of SMED30029134 vs. Ensembl Medaka
Match: dnase2b (deoxyribonuclease-2-beta [Source:NCBI gene;Acc:101174093])

HSP 1 Score: 53.9138 bits (128), Expect = 4.839e-9
Identity = 35/105 (33.33%), Postives = 56/105 (53.33%), Query Frame = -2 
Query:  113 DIYADFMAPKLKISMDVXX-XXXXXXXXXSCQSKYKVYNVRSISIDGK-KYKSTQDHCKWAVSTSIEYPFVCIGDVNRMYSQFKRGGGSLCVKNDKIWSLFQKIV 421
            DIY  ++A  L   + VES   +   + S+C       N++ I +    +++S  DH KW VS + E    C+GD+NR  +Q  RGGG +C  N  I+  F+++V
Sbjct:  242 DIYTGWVAQVLDTDLLVESWQTQGRVLPSNCSLPRHAMNIKRIQLPTLVRFQSYYDHSKWCVSQAYEDQVTCLGDLNRERAQLWRGGGLICSDNPVIYKAFRQLV 346
[Close]
BLAST of SMED30029134 vs. Planmine SMEST
Match: SMESG000081053.1 (SMESG000081053.1)

HSP 1 Score: 216.468 bits (550), Expect = 7.287e-74
Identity = 119/119 (100.00%), Postives = 119/119 (100.00%), Query Frame = -2 
Query:   47 MAPKLKISMDVXXXXXXXXXXXSCQSKYKVYNVRSISIDGKKYKSTQDHCKWAVSTSIEYPFVCIGDVNRMYSQFKRGGGSLCVKNDKIWSLFQKIVREIEPCPKLQKDIREIFKDIIL 403
            MAPKLKISMDVESWIRSSRIESSCQSKYKVYNVRSISIDGKKYKSTQDHCKWAVSTSIEYPFVCIGDVNRMYSQFKRGGGSLCVKNDKIWSLFQKIVREIEPCPKLQKDIREIFKDIIL
Sbjct:    1 MAPKLKISMDVESWIRSSRIESSCQSKYKVYNVRSISIDGKKYKSTQDHCKWAVSTSIEYPFVCIGDVNRMYSQFKRGGGSLCVKNDKIWSLFQKIVREIEPCPKLQKDIREIFKDIIL 119
[Close]
BLAST of SMED30029134 vs. Planmine SMEST
Match: SMESG000081053.1 (SMESG000081053.1)

HSP 1 Score: 201.06 bits (510), Expect = 5.714e-68
Identity = 111/111 (100.00%), Postives = 111/111 (100.00%), Query Frame = -2 
Query:   47 MDVXXXXXXXXXXXSCQSKYKVYNVRSISIDGKKYKSTQDHCKWAVSTSIEYPFVCIGDVNRMYSQFKRGGGSLCVKNDKIWSLFQKIVREIEPCPKLQKDIREIFKDIIL 379
            MDVESWIRSSRIESSCQSKYKVYNVRSISIDGKKYKSTQDHCKWAVSTSIEYPFVCIGDVNRMYSQFKRGGGSLCVKNDKIWSLFQKIVREIEPCPKLQKDIREIFKDIIL
Sbjct:    1 MDVESWIRSSRIESSCQSKYKVYNVRSISIDGKKYKSTQDHCKWAVSTSIEYPFVCIGDVNRMYSQFKRGGGSLCVKNDKIWSLFQKIVREIEPCPKLQKDIREIFKDIIL 111
[Close]
BLAST of SMED30029134 vs. Planmine SMEST
Match: SMESG000062993.1 (SMESG000062993.1)

HSP 1 Score: 118.627 bits (296), Expect = 7.189e-33
Identity = 58/112 (51.79%), Postives = 81/112 (72.32%), Query Frame = -2 
Query:   86 DIYADFMAPKLKISMDVXXXXXXXXXXXSCQSKYKVYNVRSISIDGKKYKSTQDHCKWAVSTSIEYPFVCIGDVNRMYSQFKRGGGSLCVKNDKIWSLFQKIVREIEPCPKL 421
            D+Y+  +AP L+ S+ VESWIR  +++S+C + Y VYNV  +++ G K++ + DH KWAVS + + P+VCIGD+NRM SQFKRGGG+LCVKN  IW +FQ IV + E C   
Sbjct:  227 DLYSGLVAPGLQASIMVESWIRGEKVDSTCGTDYHVYNVEKVNLPGAKFRESHDHSKWAVSNNQDSPYVCIGDINRMTSQFKRGGGTLCVKNKNIWKIFQPIVADFEKCANF 338
[Close]
BLAST of SMED30029134 vs. Planmine SMEST
Match: SMESG000062594.1 (SMESG000062594.1)

HSP 1 Score: 105.916 bits (263), Expect = 3.837e-28
Identity = 56/111 (50.45%), Postives = 75/111 (67.57%), Query Frame = -2 
Query:   95 DIYADFMAPKLKISMDVXX--XXXXXXXXXSCQSKYKVYNVRSISIDGKKYKSTQDHCKWAVSTSIEYPFVCIGDVNRMYSQFKRGGGSLCVKNDKIWSLFQKIVREIEPC 421
            D+Y+  +AP L  S+ VE+    R S+++S+C+ KYKV NV  I++  K +KST DH KWA+S S   P++CIGD+NRM SQFKRGGGSLC  N  IW  F K ++  E C
Sbjct:  232 DLYSKLIAPTLNGSLFVETWRNGRGSKLDSACELKYKVNNVEEINLSNKSFKSTSDHSKWAISQSKSSPYICIGDINRMESQFKRGGGSLCTSNVNIWEKFYKSIKTYEKC 342
[Close]
BLAST of SMED30029134 vs. Planmine SMEST
Match: SMESG000081036.1 (SMESG000081036.1)

HSP 1 Score: 94.7449 bits (234), Expect = 5.394e-24
Identity = 52/111 (46.85%), Postives = 69/111 (62.16%), Query Frame = -2 
Query:   95 DIYADFMAPKLKISMDVXX--XXXXXXXXXSCQSKYKVYNVRSISIDGKKYKSTQDHCKWAVSTSIEYPFVCIGDVNRMYSQFKRGGGSLCVKNDKIWSLFQKIVREIEPC 421
            D+Y+  +AP+LK S+ VES       R  S+CQ  YKVYN++++ I G +    +DH KWAV+ S      CIGD+NRM SQF RGGGSLC  +  +WS F K V + E C
Sbjct:  210 DLYSVLIAPQLKESLFVESWLNGNGGREPSNCQLTYKVYNIKTVKILGVEINDVRDHSKWAVTLSNATSATCIGDINRMESQFYRGGGSLCFIHANVWSAFNKAVSDYEKC 320
The following BLAST results are available for this feature:
BLAST of SMED30029134 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 3
Match NameE-valueIdentityDescription
DNASE26.776e-1731.20deoxyribonuclease 2, lysosomal [Source:HGNC Symbol... [more]
DNASE2B3.326e-1436.61deoxyribonuclease 2 beta [Source:HGNC Symbol;Acc:H... [more]
DNASE2B3.889e-1336.61deoxyribonuclease 2 beta [Source:HGNC Symbol;Acc:H... [more]
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BLAST of SMED30029134 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
crn-77.673e-2339.64Cell-death-related nuclease 7 [Source:UniProtKB/S... [more]
nuc-19.404e-1537.74Deoxyribonuclease-2 [Source:UniProtKB/Swiss-Prot;... [more]
crn-68.694e-1335.71Cell death-related nuclease 6 [Source:UniProtKB/S... [more]
crn-68.694e-1335.71Cell death-related nuclease 6 [Source:UniProtKB/S... [more]
crn-68.861e-1335.71Cell death-related nuclease 6 [Source:UniProtKB/S... [more]
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BLAST of SMED30029134 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1
Match NameE-valueIdentityDescription
DNaseII6.486e-1534.40gene:FBgn0000477 transcript:FBtr0083538[more]
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BLAST of SMED30029134 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 4
Match NameE-valueIdentityDescription
dnase21.777e-1233.63deoxyribonuclease II, lysosomal [Source:ZFIN;Acc:Z... [more]
dnase21.777e-1233.63deoxyribonuclease II, lysosomal [Source:ZFIN;Acc:Z... [more]
dnase2b2.028e-632.43deoxyribonuclease II beta [Source:ZFIN;Acc:ZDB-GEN... [more]
dnase2b2.148e-632.43deoxyribonuclease II beta [Source:ZFIN;Acc:ZDB-GEN... [more]
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BLAST of SMED30029134 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2
Match NameE-valueIdentityDescription
dnase2b4.164e-1636.75deoxyribonuclease II beta [Source:NCBI gene;Acc:49... [more]
dnase21.510e-930.86deoxyribonuclease II, lysosomal [Source:NCBI gene;... [more]
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BLAST of SMED30029134 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 4
Match NameE-valueIdentityDescription
Dnase2a1.277e-1430.09deoxyribonuclease II alpha [Source:MGI Symbol;Acc:... [more]
Dnase2a1.539e-1430.09deoxyribonuclease II alpha [Source:MGI Symbol;Acc:... [more]
Dnase2b1.327e-1339.05deoxyribonuclease II beta [Source:MGI Symbol;Acc:M... [more]
Dnase2b1.327e-1339.05deoxyribonuclease II beta [Source:MGI Symbol;Acc:M... [more]
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BLAST of SMED30029134 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P34387|TG198_CAEEL9.761e-2239.64Cell-death-related nuclease 7 OS=Caenorhabditis el... [more]
sp|Q75WF2|DNA2_ACAPL1.294e-1643.21Plancitoxin-1 OS=Acanthaster planci OX=133434 PE=1... [more]
sp|O00115|DNS2A_HUMAN3.254e-1631.20Deoxyribonuclease-2-alpha OS=Homo sapiens OX=9606 ... [more]
sp|Q9QZK8|DNS2A_RAT5.585e-1430.97Deoxyribonuclease-2-alpha OS=Rattus norvegicus OX=... [more]
sp|P56542|DNS2A_MOUSE1.076e-1330.09Deoxyribonuclease-2-alpha OS=Mus musculus OX=10090... [more]
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BLAST of SMED30029134 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0B2W4P73.453e-2750.00Cell-death-related nuclease 7 OS=Toxocara canis OX... [more]
A0A0M3KCV07.659e-2641.90Cell-death-related nuclease 7 (inferred by ortholo... [more]
A0A3P6TGL99.420e-2641.90Uncharacterized protein OS=Anisakis simplex OX=626... [more]
F1L8241.424e-2539.85Cell-death-related nuclease 7 OS=Ascaris suum OX=6... [more]
A0A443S1643.058e-2442.15Plancitoxin-1-like protein (Fragment) OS=Leptotrom... [more]
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BLAST of SMED30029134 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
dnase24.572e-1636.94deoxyribonuclease-2-alpha-like [Source:NCBI gene;A... [more]
dnase2b1.023e-1132.43deoxyribonuclease 2 beta [Source:NCBI gene;Acc:103... [more]
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BLAST of SMED30029134 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
dnase23.232e-1735.83deoxyribonuclease II, lysosomal [Source:ZFIN;Acc:Z... [more]
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BLAST of SMED30029134 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30029134 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30029134 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 3
Match NameE-valueIdentityDescription
ENSORLT00000036979.15.500e-1234.18deoxyribonuclease-2-alpha [Source:NCBI gene;Acc:10... [more]
dnase21.253e-1130.17deoxyribonuclease 2, lysosomal [Source:NCBI gene;A... [more]
dnase2b4.839e-933.33deoxyribonuclease-2-beta [Source:NCBI gene;Acc:101... [more]
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BLAST of SMED30029134 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000081053.17.287e-74100.00SMESG000081053.1[more]
SMESG000081053.15.714e-68100.00SMESG000081053.1[more]
SMESG000062993.17.189e-3351.79SMESG000062993.1[more]
SMESG000062594.13.837e-2850.45SMESG000062594.1[more]
SMESG000081036.15.394e-2446.85SMESG000081036.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30029134 ID=SMED30029134|Name=SMED30029134|organism=Schmidtea mediterranea sexual|type=transcript|length=425bp
AAAGTTTTAAATAAATAATTAGTTGCTATAAATTATTTTATTTTCACAAT
ATAATATCCTTGAAAATTTCACGAATATCTTTCTGGAGTTTTGGACAAGG
CTCGATTTCCCTCACGATTTTTTGGAAGAGACTCCAAATTTTATCATTTT
TAACACACAAACTACCCCCACCTCTTTTAAATTGAGAATACATTCGATTG
ACATCTCCAATACAGACAAAAGGATATTCTATGGAAGTTGAAACAGCCCA
TTTGCAATGATCCTGTGTTGATTTGTACTTCTTGCCATCGATGGAAATAC
TCCTCACGTTGTAAACTTTATACTTAGATTGGCATGAAGACTCGATTCTA
GAACTCCTGATCCATGATTCAACATCCATTGAGATTTTTAGTTTTGGGGC
CATAAAATCCGCATAAATATCTAAA
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protein sequence of SMED30029134-orf-1

>SMED30029134-orf-1 ID=SMED30029134-orf-1|Name=SMED30029134-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=127bp
LDIYADFMAPKLKISMDVESWIRSSRIESSCQSKYKVYNVRSISIDGKKY
KSTQDHCKWAVSTSIEYPFVCIGDVNRMYSQFKRGGGSLCVKNDKIWSLF
QKIVREIEPCPKLQKDIREIFKDIIL*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR004947DNase_II
Vocabulary: molecular function
TermDefinition
GO:0004531deoxyribonuclease II activity
Vocabulary: biological process
TermDefinition
GO:0006259DNA metabolic process
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004947Deoxyribonuclease IIPFAMPF03265DNase_IIcoord: 2..110
e-value: 3.7E-36
score: 125.1
IPR004947Deoxyribonuclease IIPANTHERPTHR10858DEOXYRIBONUCLEASE IIcoord: 2..113
NoneNo IPR availablePANTHERPTHR10858:SF23DEOXYRIBONUCLEASE IIcoord: 2..113