Gamma-secretase subunit PEN-2

Overview
NameGamma-secretase subunit PEN-2
Smed IDSMED30029116
Length (bp)1247
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Gamma-secretase subunit PEN-2 (SMED30029116) t-SNE clustered cells

Violin plots show distribution of expression levels for Gamma-secretase subunit PEN-2 (SMED30029116) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Gamma-secretase subunit PEN-2 (SMED30029116) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Gamma-secretase subunit PEN-2 (SMED30029116) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Homology
[Close]
BLAST of Gamma-secretase subunit PEN-2 vs. Ensembl Human
Match: PSENEN (presenilin enhancer, gamma-secretase subunit [Source:HGNC Symbol;Acc:HGNC:30100])

HSP 1 Score: 82.0333 bits (201), Expect = 1.277e-18
Identity = 41/99 (41.41%), Postives = 60/99 (60.61%), Query Frame = 2 
Query:  581 MDINSVSGSEKLSICKKYFYGGIFCLPFMWLINSLWFFKLAFQAEQFPEQGQIKRYVIGSMIGTTCWTVAIFTWIIKYQMVS-RLMQISS-LTFNVPLG 871
            M++  VS  EKL++C+KY+ GG   LPF+WL+N  WFF+ AF    + EQ QIK YV  S +G   W + + +WI  +Q+   R   +   L+F +PLG
Sbjct:    1 MNLERVSNEEKLNLCRKYYLGGFAFLPFLWLVNIFWFFREAFLVPAYTEQSQIKGYVWRSAVGFLFWVIVLTSWITIFQIYRPRWGALGDYLSFTIPLG 99
[Close]
BLAST of Gamma-secretase subunit PEN-2 vs. Ensembl Human
Match: PSENEN (presenilin enhancer, gamma-secretase subunit [Source:HGNC Symbol;Acc:HGNC:30100])

HSP 1 Score: 82.0333 bits (201), Expect = 1.277e-18
Identity = 41/99 (41.41%), Postives = 60/99 (60.61%), Query Frame = 2 
Query:  581 MDINSVSGSEKLSICKKYFYGGIFCLPFMWLINSLWFFKLAFQAEQFPEQGQIKRYVIGSMIGTTCWTVAIFTWIIKYQMVS-RLMQISS-LTFNVPLG 871
            M++  VS  EKL++C+KY+ GG   LPF+WL+N  WFF+ AF    + EQ QIK YV  S +G   W + + +WI  +Q+   R   +   L+F +PLG
Sbjct:    1 MNLERVSNEEKLNLCRKYYLGGFAFLPFLWLVNIFWFFREAFLVPAYTEQSQIKGYVWRSAVGFLFWVIVLTSWITIFQIYRPRWGALGDYLSFTIPLG 99
[Close]
BLAST of Gamma-secretase subunit PEN-2 vs. Ensembl Human
Match: AD000671.1 (novel protein)

HSP 1 Score: 63.5438 bits (153), Expect = 1.516e-12
Identity = 27/54 (50.00%), Postives = 37/54 (68.52%), Query Frame = 2 
Query:  581 MDINSVSGSEKLSICKKYFYGGIFCLPFMWLINSLWFFKLAFQAEQFPEQGQIK 742
            M++  VS  EKL++C+KY+ GG   LPF+WL+N  WFF+ AF    + EQ QIK
Sbjct:    1 MNLERVSNEEKLNLCRKYYLGGFAFLPFLWLVNIFWFFREAFLVPAYTEQSQIK 54
[Close]
BLAST of Gamma-secretase subunit PEN-2 vs. Ensembl Human
Match: PSENEN (presenilin enhancer, gamma-secretase subunit [Source:HGNC Symbol;Acc:HGNC:30100])

HSP 1 Score: 64.6994 bits (156), Expect = 1.891e-12
Identity = 27/54 (50.00%), Postives = 37/54 (68.52%), Query Frame = 2 
Query:  581 MDINSVSGSEKLSICKKYFYGGIFCLPFMWLINSLWFFKLAFQAEQFPEQGQIK 742
            M++  VS  EKL++C+KY+ GG   LPF+WL+N  WFF+ AF    + EQ QIK
Sbjct:    1 MNLERVSNEEKLNLCRKYYLGGFAFLPFLWLVNIFWFFREAFLVPAYTEQSQIK 54
[Close]
BLAST of Gamma-secretase subunit PEN-2 vs. Ensembl Celegans
Match: pen-2 (Gamma-secretase subunit pen-2 [Source:UniProtKB/Swiss-Prot;Acc:Q9U357])

HSP 1 Score: 70.4774 bits (171), Expect = 7.717e-15
Identity = 36/99 (36.36%), Postives = 56/99 (56.57%), Query Frame = 2 
Query:  581 MDINSVSGSEKLSICKKYFYGGIFCLPFMWLINSLWFFKLAFQAEQFPEQGQIKRYVIGSMIGTTCWTVAIFTWIIKYQMVSR--LMQISSLTFNVPLG 871
            MDI+ ++  +K+ +CKKYF  G   LP +W++N+ WFF  AF       + QI++YVI S++G+  W + +  W I +Q      L+    LTF  P G
Sbjct:    1 MDISKLTDVKKVDLCKKYFLIGACFLPLVWIVNTFWFFSDAFCKPINAHRRQIRKYVIASIVGSIFWIIVLSAWEIFFQHYRAQGLVWTDFLTFVFPTG 99
[Close]
BLAST of Gamma-secretase subunit PEN-2 vs. Ensembl Fly
Match: pen-2 (gene:FBgn0053198 transcript:FBtr0340331)

HSP 1 Score: 87.0409 bits (214), Expect = 1.116e-20
Identity = 43/100 (43.00%), Postives = 57/100 (57.00%), Query Frame = 2 
Query:  581 MDINSVSGSEKLSICKKYFYGGIFCLPFMWLINSLWFFKLAFQAEQFPEQGQIKRYVIGSMIGTTCWTVAIFTWIIKYQMVSRLMQISS--LTFNVPLGG 874
            MDI+      KL +C+KYF+ G   LPF+W IN  WFF  AF    F EQ QIKRYVI S +GT  W + +  WII +Q        ++  ++F +PLG 
Sbjct:    1 MDISKAPNPRKLELCRKYFFAGFAFLPFVWAINVCWFFTEAFHKPPFSEQSQIKRYVIYSAVGTLFWLIVLTAWIIIFQTNRTAWGATADYMSFIIPLGS 100
[Close]
BLAST of Gamma-secretase subunit PEN-2 vs. Ensembl Fly
Match: pen-2 (gene:FBgn0053198 transcript:FBtr0089842)

HSP 1 Score: 87.0409 bits (214), Expect = 1.116e-20
Identity = 43/100 (43.00%), Postives = 57/100 (57.00%), Query Frame = 2 
Query:  581 MDINSVSGSEKLSICKKYFYGGIFCLPFMWLINSLWFFKLAFQAEQFPEQGQIKRYVIGSMIGTTCWTVAIFTWIIKYQMVSRLMQISS--LTFNVPLGG 874
            MDI+      KL +C+KYF+ G   LPF+W IN  WFF  AF    F EQ QIKRYVI S +GT  W + +  WII +Q        ++  ++F +PLG 
Sbjct:    1 MDISKAPNPRKLELCRKYFFAGFAFLPFVWAINVCWFFTEAFHKPPFSEQSQIKRYVIYSAVGTLFWLIVLTAWIIIFQTNRTAWGATADYMSFIIPLGS 100
[Close]
BLAST of Gamma-secretase subunit PEN-2 vs. Ensembl Zebrafish
Match: psenen (presenilin enhancer, gamma-secretase subunit [Source:NCBI gene;Acc:402810])

HSP 1 Score: 84.3445 bits (207), Expect = 1.338e-19
Identity = 41/99 (41.41%), Postives = 60/99 (60.61%), Query Frame = 2 
Query:  581 MDINSVSGSEKLSICKKYFYGGIFCLPFMWLINSLWFFKLAFQAEQFPEQGQIKRYVIGSMIGTTCWTVAIFTWIIKYQMV-SRLMQISS-LTFNVPLG 871
            M++  +   EKLS+C++Y+ GG   LPF+WL+N LWFFK AF    + EQ QIK YV  S +G   W   + TWI  +Q   ++  ++   L+F +PLG
Sbjct:    1 MNLERIPNEEKLSLCRRYYLGGFAFLPFLWLVNILWFFKEAFLKPAYTEQPQIKSYVKKSALGLLLWVAVLTTWITVFQHFRAQWGEVGDYLSFTIPLG 99
[Close]
BLAST of Gamma-secretase subunit PEN-2 vs. Ensembl Zebrafish
Match: psenen (presenilin enhancer, gamma-secretase subunit [Source:NCBI gene;Acc:402810])

HSP 1 Score: 84.3445 bits (207), Expect = 1.338e-19
Identity = 41/99 (41.41%), Postives = 60/99 (60.61%), Query Frame = 2 
Query:  581 MDINSVSGSEKLSICKKYFYGGIFCLPFMWLINSLWFFKLAFQAEQFPEQGQIKRYVIGSMIGTTCWTVAIFTWIIKYQMV-SRLMQISS-LTFNVPLG 871
            M++  +   EKLS+C++Y+ GG   LPF+WL+N LWFFK AF    + EQ QIK YV  S +G   W   + TWI  +Q   ++  ++   L+F +PLG
Sbjct:    1 MNLERIPNEEKLSLCRRYYLGGFAFLPFLWLVNILWFFKEAFLKPAYTEQPQIKSYVKKSALGLLLWVAVLTTWITVFQHFRAQWGEVGDYLSFTIPLG 99
[Close]
BLAST of Gamma-secretase subunit PEN-2 vs. Ensembl Xenopus
Match: psenen (presenilin enhancer, gamma-secretase subunit [Source:NCBI gene;Acc:549090])

HSP 1 Score: 79.337 bits (194), Expect = 1.040e-17
Identity = 41/99 (41.41%), Postives = 56/99 (56.57%), Query Frame = 2 
Query:  581 MDINSVSGSEKLSICKKYFYGGIFCLPFMWLINSLWFFKLAFQAEQFPEQGQIKRYVIGSMIGTTCWTVAIFTWIIKYQMVSRLMQISS--LTFNVPLG 871
            M++  V   EKL +C+KY+ GG   LPF+WL+N +WFFK AF    + EQ  I+ YV  S +G   W V + TWI  YQ        +   L+F +PLG
Sbjct:    1 MNLERVPNEEKLQLCRKYYLGGFALLPFLWLVNVVWFFKEAFFKPAYTEQPLIQSYVKRSALGLFVWVVILTTWISVYQTHRAGWGATGDYLSFTIPLG 99
[Close]
BLAST of Gamma-secretase subunit PEN-2 vs. Ensembl Mouse
Match: Psenen (presenilin enhancer gamma secretase subunit [Source:MGI Symbol;Acc:MGI:1913590])

HSP 1 Score: 84.7297 bits (208), Expect = 8.675e-20
Identity = 43/99 (43.43%), Postives = 61/99 (61.62%), Query Frame = 2 
Query:  581 MDINSVSGSEKLSICKKYFYGGIFCLPFMWLINSLWFFKLAFQAEQFPEQGQIKRYVIGSMIGTTCWTVAIFTWIIKYQMV-SRLMQISS-LTFNVPLG 871
            M++  VS  EKL++C+KY+ GG   LPF+WL+N  WFF+ AF A  + EQ QIK YV  S +G   W + + TWI  +Q+   R   +   L+F +PLG
Sbjct:    1 MNLERVSNEEKLNLCRKYYLGGFAFLPFLWLVNIFWFFREAFLAPAYTEQSQIKGYVWRSAVGFLFWVIILATWITIFQIYRPRWGALGDYLSFTIPLG 99
[Close]
BLAST of Gamma-secretase subunit PEN-2 vs. Ensembl Mouse
Match: Psenen (presenilin enhancer gamma secretase subunit [Source:MGI Symbol;Acc:MGI:1913590])

HSP 1 Score: 84.7297 bits (208), Expect = 8.675e-20
Identity = 43/99 (43.43%), Postives = 61/99 (61.62%), Query Frame = 2 
Query:  581 MDINSVSGSEKLSICKKYFYGGIFCLPFMWLINSLWFFKLAFQAEQFPEQGQIKRYVIGSMIGTTCWTVAIFTWIIKYQMV-SRLMQISS-LTFNVPLG 871
            M++  VS  EKL++C+KY+ GG   LPF+WL+N  WFF+ AF A  + EQ QIK YV  S +G   W + + TWI  +Q+   R   +   L+F +PLG
Sbjct:    1 MNLERVSNEEKLNLCRKYYLGGFAFLPFLWLVNIFWFFREAFLAPAYTEQSQIKGYVWRSAVGFLFWVIILATWITIFQIYRPRWGALGDYLSFTIPLG 99
[Close]
BLAST of Gamma-secretase subunit PEN-2 vs. Ensembl Mouse
Match: Psenen (presenilin enhancer gamma secretase subunit [Source:MGI Symbol;Acc:MGI:1913590])

HSP 1 Score: 81.2629 bits (199), Expect = 1.407e-18
Identity = 37/80 (46.25%), Postives = 52/80 (65.00%), Query Frame = 2 
Query:  581 MDINSVSGSEKLSICKKYFYGGIFCLPFMWLINSLWFFKLAFQAEQFPEQGQIKRYVIGSMIGTTCWTVAIFTWIIKYQM 820
            M++  VS  EKL++C+KY+ GG   LPF+WL+N  WFF+ AF A  + EQ QIK YV  S +G   W + + TWI  +Q+
Sbjct:    1 MNLERVSNEEKLNLCRKYYLGGFAFLPFLWLVNIFWFFREAFLAPAYTEQSQIKGYVWRSAVGFLFWVIILATWITIFQI 80
[Close]
BLAST of Gamma-secretase subunit PEN-2 vs. Ensembl Mouse
Match: Psenen (presenilin enhancer gamma secretase subunit [Source:MGI Symbol;Acc:MGI:1913590])

HSP 1 Score: 48.521 bits (114), Expect = 1.488e-7
Identity = 21/41 (51.22%), Postives = 30/41 (73.17%), Query Frame = 2 
Query:  581 MDINSVSGSEKLSICKKYFYGGIFCLPFMWLINSLWFFKLA 703
            M++  VS  EKL++C+KY+ GG   LPF+WL+N  WFF+ A
Sbjct:    1 MNLERVSNEEKLNLCRKYYLGGFAFLPFLWLVNIFWFFREA 41
[Close]
BLAST of Gamma-secretase subunit PEN-2 vs. UniProt/SwissProt
Match: sp|Q86BE9|PEN2_DROME (Gamma-secretase subunit pen-2 OS=Drosophila melanogaster OX=7227 GN=pen-2 PE=1 SV=3)

HSP 1 Score: 87.0409 bits (214), Expect = 1.119e-19
Identity = 43/100 (43.00%), Postives = 57/100 (57.00%), Query Frame = 2 
Query:  581 MDINSVSGSEKLSICKKYFYGGIFCLPFMWLINSLWFFKLAFQAEQFPEQGQIKRYVIGSMIGTTCWTVAIFTWIIKYQMVSRLMQISS--LTFNVPLGG 874
            MDI+      KL +C+KYF+ G   LPF+W IN  WFF  AF    F EQ QIKRYVI S +GT  W + +  WII +Q        ++  ++F +PLG 
Sbjct:    1 MDISKAPNPRKLELCRKYFFAGFAFLPFVWAINVCWFFTEAFHKPPFSEQSQIKRYVIYSAVGTLFWLIVLTAWIIIFQTNRTAWGATADYMSFIIPLGS 100
[Close]
BLAST of Gamma-secretase subunit PEN-2 vs. UniProt/SwissProt
Match: sp|Q6QI68|PEN2_RAT (Gamma-secretase subunit PEN-2 OS=Rattus norvegicus OX=10116 GN=Psenen PE=3 SV=1)

HSP 1 Score: 85.5001 bits (210), Expect = 3.718e-19
Identity = 44/99 (44.44%), Postives = 61/99 (61.62%), Query Frame = 2 
Query:  581 MDINSVSGSEKLSICKKYFYGGIFCLPFMWLINSLWFFKLAFQAEQFPEQGQIKRYVIGSMIGTTCWTVAIFTWIIKYQMV-SRLMQISS-LTFNVPLG 871
            M++  VS  EKL++C+KY+ GG   LPF+WL+N  WFFK AF A  + EQ QIK YV  S +G   W + + TWI  +Q+   R   +   L+F +PLG
Sbjct:    1 MNLERVSNEEKLNLCRKYYLGGFAFLPFLWLVNIFWFFKEAFFAPAYTEQSQIKGYVWRSAVGFLFWVIVLTTWITIFQIYRPRWGALGDYLSFTIPLG 99
[Close]
BLAST of Gamma-secretase subunit PEN-2 vs. UniProt/SwissProt
Match: sp|Q9CQR7|PEN2_MOUSE (Gamma-secretase subunit PEN-2 OS=Mus musculus OX=10090 GN=Psenen PE=1 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 6.068e-19
Identity = 43/99 (43.43%), Postives = 61/99 (61.62%), Query Frame = 2 
Query:  581 MDINSVSGSEKLSICKKYFYGGIFCLPFMWLINSLWFFKLAFQAEQFPEQGQIKRYVIGSMIGTTCWTVAIFTWIIKYQMV-SRLMQISS-LTFNVPLG 871
            M++  VS  EKL++C+KY+ GG   LPF+WL+N  WFF+ AF A  + EQ QIK YV  S +G   W + + TWI  +Q+   R   +   L+F +PLG
Sbjct:    1 MNLERVSNEEKLNLCRKYYLGGFAFLPFLWLVNIFWFFREAFLAPAYTEQSQIKGYVWRSAVGFLFWVIILATWITIFQIYRPRWGALGDYLSFTIPLG 99
[Close]
BLAST of Gamma-secretase subunit PEN-2 vs. UniProt/SwissProt
Match: sp|Q8JHF0|PEN2_DANRE (Gamma-secretase subunit PEN-2 OS=Danio rerio OX=7955 GN=psenen PE=3 SV=1)

HSP 1 Score: 84.3445 bits (207), Expect = 1.010e-18
Identity = 41/99 (41.41%), Postives = 60/99 (60.61%), Query Frame = 2 
Query:  581 MDINSVSGSEKLSICKKYFYGGIFCLPFMWLINSLWFFKLAFQAEQFPEQGQIKRYVIGSMIGTTCWTVAIFTWIIKYQMV-SRLMQISS-LTFNVPLG 871
            M++  +   EKLS+C++Y+ GG   LPF+WL+N LWFFK AF    + EQ QIK YV  S +G   W   + TWI  +Q   ++  ++   L+F +PLG
Sbjct:    1 MNLERIPNEEKLSLCRRYYLGGFAFLPFLWLVNILWFFKEAFLKPAYTEQPQIKSYVKKSALGLLLWVAVLTTWITVFQHFRAQWGEVGDYLSFTIPLG 99
[Close]
BLAST of Gamma-secretase subunit PEN-2 vs. UniProt/SwissProt
Match: sp|Q5G235|PEN2_BOVIN (Gamma-secretase subunit PEN-2 OS=Bos taurus OX=9913 GN=PSENEN PE=3 SV=1)

HSP 1 Score: 82.8037 bits (203), Expect = 2.995e-18
Identity = 42/99 (42.42%), Postives = 60/99 (60.61%), Query Frame = 2 
Query:  581 MDINSVSGSEKLSICKKYFYGGIFCLPFMWLINSLWFFKLAFQAEQFPEQGQIKRYVIGSMIGTTCWTVAIFTWIIKYQMVS-RLMQISS-LTFNVPLG 871
            M++  VS  EKL++C+KY+ GG   LPF+WL+N  WFF+ AF    + EQ QIK YV  S +G   W + + TWI  +Q+   R   +   L+F +PLG
Sbjct:    1 MNLERVSNEEKLNLCRKYYLGGFAFLPFLWLVNIFWFFREAFIVPAYTEQSQIKGYVWRSAVGFFLWVIVLSTWITIFQIYRPRWGALGDYLSFTIPLG 99
[Close]
BLAST of Gamma-secretase subunit PEN-2 vs. TrEMBL
Match: A0A3S0ZZ85 (Uncharacterized protein OS=Elysia chlorotica OX=188477 GN=EGW08_013386 PE=4 SV=1)

HSP 1 Score: 98.2117 bits (243), Expect = 2.191e-21
Identity = 43/80 (53.75%), Postives = 56/80 (70.00%), Query Frame = 2 
Query:  581 MDINSVSGSEKLSICKKYFYGGIFCLPFMWLINSLWFFKLAFQAEQFPEQGQIKRYVIGSMIGTTCWTVAIFTWIIKYQM 820
            MD+  V+ +EKL +C++Y+ GG   LPF+WLINS+WFFK AF   Q+ EQ QIK YVI SMIG   W  A+ TWI  +Q+
Sbjct:    1 MDLRRVNNAEKLVMCRRYYLGGFLVLPFLWLINSVWFFKEAFMKPQYTEQAQIKSYVIRSMIGCVVWLAALITWITIFQL 80
[Close]
BLAST of Gamma-secretase subunit PEN-2 vs. TrEMBL
Match: A0A2C9JEK1 (Uncharacterized protein OS=Biomphalaria glabrata OX=6526 GN=106055139 PE=4 SV=1)

HSP 1 Score: 96.2857 bits (238), Expect = 1.196e-20
Identity = 44/99 (44.44%), Postives = 64/99 (64.65%), Query Frame = 2 
Query:  581 MDINSVSGSEKLSICKKYFYGGIFCLPFMWLINSLWFFKLAFQAEQFPEQGQIKRYVIGSMIGTTCWTVAIFTWIIKYQM--VSRLMQISSLTFNVPLG 871
            MD+  +   +KL +C+KY+  G F LPF+WL+N++WFFK AF+   + EQ +I+ YV  S+IG+  W  AI TW+I +Q+  VS       LTF +PLG
Sbjct:    1 MDLQKLKNEDKLILCRKYYLAGFFLLPFLWLVNAIWFFKEAFRKPHYAEQSEIRTYVTRSIIGSAIWLAAIITWVIIFQLNRVSWGATGDLLTFVIPLG 99
[Close]
BLAST of Gamma-secretase subunit PEN-2 vs. TrEMBL
Match: A0A1I8IIK8 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 99.3673 bits (246), Expect = 6.033e-20
Identity = 44/80 (55.00%), Postives = 57/80 (71.25%), Query Frame = 2 
Query:  581 MDINSVSGSEKLSICKKYFYGGIFCLPFMWLINSLWFFKLAFQAEQFPEQGQIKRYVIGSMIGTTCWTVAIFTWIIKYQM 820
            MD++  SG++KL+IC+KY  GG F LPF+WL N  WFF+ AF A  +PEQ QI+R VI S IG+  WTVA+ TW   YQ+
Sbjct:    1 MDLSKASGADKLNICRKYTIGGAFLLPFLWLTNFAWFFREAFLAPAYPEQAQIRRLVIISGIGSAIWTVALLTWFCVYQV 80
[Close]
BLAST of Gamma-secretase subunit PEN-2 vs. TrEMBL
Match: F6VQU7 (Uncharacterized protein OS=Monodelphis domestica OX=13616 GN=PSENEN PE=4 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 1.769e-19
Identity = 47/99 (47.47%), Postives = 65/99 (65.66%), Query Frame = 2 
Query:  581 MDINSVSGSEKLSICKKYFYGGIFCLPFMWLINSLWFFKLAFQAEQFPEQGQIKRYVIGSMIGTTCWTVAIFTWIIKYQM-VSRLMQISS-LTFNVPLG 871
            M++  VS  EKL++C+KY+ GG   LPF+WL+N LWFF+ AF A  + EQGQIK YV  S +G   W VA+ +WI  +Q+  +R   I   L+F +PLG
Sbjct:    1 MNLERVSNEEKLNLCRKYYLGGFALLPFLWLVNVLWFFREAFLAPAYEEQGQIKGYVWRSALGLVFWLVALTSWITIFQINRARWGAIGDYLSFTIPLG 99
[Close]
BLAST of Gamma-secretase subunit PEN-2 vs. TrEMBL
Match: S4RAK4 (Presenilin enhancer, gamma-secretase subunit OS=Petromyzon marinus OX=7757 PE=4 SV=1)

HSP 1 Score: 92.8189 bits (229), Expect = 2.520e-19
Identity = 42/99 (42.42%), Postives = 62/99 (62.63%), Query Frame = 2 
Query:  581 MDINSVSGSEKLSICKKYFYGGIFCLPFMWLINSLWFFKLAFQAEQFPEQGQIKRYVIGSMIGTTCWTVAIFTWIIKYQMVSRLMQISS--LTFNVPLG 871
            M++  V+  +KLS+C+KY+ GG   LPF+WL+N++WFFK AF   Q+ EQ +IK+YVI S +G   WTV +  WI  YQ        +   ++F +P G
Sbjct:    1 MNLERVTNEDKLSLCRKYYRGGFILLPFLWLVNAIWFFKEAFMKPQYQEQAEIKKYVIRSAVGVLGWTVVLTVWISIYQHFRPQWGATGDYMSFTIPFG 99
[Close]
BLAST of Gamma-secretase subunit PEN-2 vs. Ensembl Cavefish
Match: psenen (presenilin enhancer, gamma-secretase subunit [Source:NCBI gene;Acc:103047545])

HSP 1 Score: 82.4185 bits (202), Expect = 4.718e-19
Identity = 40/99 (40.40%), Postives = 61/99 (61.62%), Query Frame = 2 
Query:  581 MDINSVSGSEKLSICKKYFYGGIFCLPFMWLINSLWFFKLAFQAEQFPEQGQIKRYVIGSMIGTTCWTVAIFTWIIKYQMV-SRLMQISS-LTFNVPLG 871
            M++  +   EKLS+C+KY+ GG   LPF+WL+N +WFFK AF    + EQ QIK YV  S +G   W   + TWI  +Q   ++  +++  ++F +PLG
Sbjct:    1 MNLERIPNEEKLSLCRKYYLGGFAFLPFLWLVNCVWFFKEAFVKPAYTEQPQIKTYVKRSALGLLLWVTVLTTWICIFQHFRAQWGEVADYISFTIPLG 99
[Close]
BLAST of Gamma-secretase subunit PEN-2 vs. Ensembl Sea Lamprey
Match: psenen (presenilin enhancer, gamma-secretase subunit [Source:NCBI gene;Acc:402810])

HSP 1 Score: 92.8189 bits (229), Expect = 1.936e-23
Identity = 42/99 (42.42%), Postives = 62/99 (62.63%), Query Frame = 2 
Query:  581 MDINSVSGSEKLSICKKYFYGGIFCLPFMWLINSLWFFKLAFQAEQFPEQGQIKRYVIGSMIGTTCWTVAIFTWIIKYQMVSRLMQISS--LTFNVPLG 871
            M++  V+  +KLS+C+KY+ GG   LPF+WL+N++WFFK AF   Q+ EQ +IK+YVI S +G   WTV +  WI  YQ        +   ++F +P G
Sbjct:    1 MNLERVTNEDKLSLCRKYYRGGFILLPFLWLVNAIWFFKEAFMKPQYQEQAEIKKYVIRSAVGVLGWTVVLTVWISIYQHFRPQWGATGDYMSFTIPFG 99
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BLAST of Gamma-secretase subunit PEN-2 vs. Ensembl Nematostella
Match: EDO46097 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RR32])

HSP 1 Score: 83.9593 bits (206), Expect = 5.104e-20
Identity = 42/99 (42.42%), Postives = 60/99 (60.61%), Query Frame = 2 
Query:  581 MDINSVSGSEKLSICKKYFYGGIFCLPFMWLINSLWFFKLAFQAEQFPEQGQIKRYVIGSMIGTTCWTVAIFTWIIKYQM--VSRLMQISSLTFNVPLG 871
            MD+  VS  EK+ +C+KY   G+  LPF+WL+N++WFF+ AF  E FP Q  ++  VI S IG + W   + TWI+ YQ    S      +L+F +PLG
Sbjct:    1 MDLRKVSDEEKVKLCRKYTLVGLAFLPFLWLVNAIWFFREAFCRESFPGQKSMRSNVIKSFIGFSIWAAGLSTWIVMYQTNRASWGELGDTLSFLIPLG 99
[Close]
BLAST of Gamma-secretase subunit PEN-2 vs. Ensembl Medaka
Match: psenen (presenilin enhancer, gamma-secretase subunit [Source:NCBI gene;Acc:101173167])

HSP 1 Score: 79.7221 bits (195), Expect = 4.658e-18
Identity = 40/99 (40.40%), Postives = 60/99 (60.61%), Query Frame = 2 
Query:  581 MDINSVSGSEKLSICKKYFYGGIFCLPFMWLINSLWFFKLAFQAEQFPEQGQIKRYVIGSMIGTTCWTVAIFTWIIKYQMV-SRLMQI-SSLTFNVPLG 871
            M++  +   EKL +C+KY+ GG   LPF+WL+N +WFF+ AF    + EQ QIK YV  S +G   W   + TWI  +Q   ++  ++  SL+F +PLG
Sbjct:    1 MNLERLPNEEKLILCRKYYLGGFAFLPFLWLVNVVWFFREAFVKPAYTEQLQIKAYVKWSAVGLLFWVAVLTTWITIFQHFRAQWGEVGDSLSFTIPLG 99
[Close]
BLAST of Gamma-secretase subunit PEN-2 vs. Planmine SMEST
Match: SMESG000035771.1 (SMESG000035771.1)

HSP 1 Score: 201.06 bits (510), Expect = 3.520e-64
Identity = 98/98 (100.00%), Postives = 98/98 (100.00%), Query Frame = 2 
Query:  581 MDINSVSGSEKLSICKKYFYGGIFCLPFMWLINSLWFFKLAFQAEQFPEQGQIKRYVIGSMIGTTCWTVAIFTWIIKYQMVSRLMQISSLTFNVPLGG 874
            MDINSVSGSEKLSICKKYFYGGIFCLPFMWLINSLWFFKLAFQAEQFPEQGQIKRYVIGSMIGTTCWTVAIFTWIIKYQMVSRLMQISSLTFNVPLGG
Sbjct:    1 MDINSVSGSEKLSICKKYFYGGIFCLPFMWLINSLWFFKLAFQAEQFPEQGQIKRYVIGSMIGTTCWTVAIFTWIIKYQMVSRLMQISSLTFNVPLGG 98
[Close]
BLAST of Gamma-secretase subunit PEN-2 vs. Planmine SMEST
Match: SMESG000015815.1 (SMESG000015815.1)

HSP 1 Score: 72.0182 bits (175), Expect = 1.985e-15
Identity = 30/69 (43.48%), Postives = 44/69 (63.77%), Query Frame = 2 
Query:  599 SGSEKLSICKKYFYGGIFCLPFMWLINSLWFFKLAFQAEQFPEQGQIKRYVIGSMIGTTCWTVAIFTWI 805
            S + KL +C+ YF+ G F LP +W IN +WFF  A+  E +PEQ +++ YVI S++G   W V I  W+
Sbjct:   12 SSAIKLKLCRLYFFVGAFGLPILWFINLMWFFDFAYNEETYPEQKKVQFYVIFSLVGVIAWIVLIIIWM 80
[Close]
BLAST of Gamma-secretase subunit PEN-2 vs. Planmine SMEST
Match: SMESG000015820.1 (SMESG000015820.1)

HSP 1 Score: 71.2478 bits (173), Expect = 3.432e-15
Identity = 33/75 (44.00%), Postives = 43/75 (57.33%), Query Frame = 2 
Query:  581 MDINSVSGSEKLSICKKYFYGGIFCLPFMWLINSLWFFKLAFQAEQFPEQGQIKRYVIGSMIGTTCWTVAIFTWI 805
            +D+  +S  EKL IC+ YF  G F LP +WL N  WFFK  FQ   F E+  IK+YV  S +G   ++  I  WI
Sbjct:    4 VDLEKISNEEKLKICRNYFIAGFFVLPILWLFNVAWFFKYGFQGPAFAEKFTIKKYVAASALGFCIYSSLIGLWI 78
[Close]
BLAST of Gamma-secretase subunit PEN-2 vs. Planmine SMEST
Match: SMESG000015821.1 (SMESG000015821.1)

HSP 1 Score: 67.3958 bits (163), Expect = 9.554e-14
Identity = 36/75 (48.00%), Postives = 51/75 (68.00%), Query Frame = 2 
Query:  581 MDINSVSGSEKLSICKKYFYGGIFCLPFMWLINSLWFFKLAFQ----AEQF-PEQGQIKRYVIGSMIGTTCWTVA 790
            ++I  ++ +EKL IC++YF  G F LP +WLIN  WFF+ AF+     EQ  PE+  IK+YVIGS+IG   W+V+
Sbjct:    4 VNIKLITNAEKLKICRQYFIAGFFLLPSLWLINIFWFFEEAFKNVPDEEQLDPEKLGIKKYVIGSLIG---WSVS 75
The following BLAST results are available for this feature:
BLAST of Gamma-secretase subunit PEN-2 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 4
Match NameE-valueIdentityDescription
PSENEN1.277e-1841.41presenilin enhancer, gamma-secretase subunit [Sour... [more]
PSENEN1.277e-1841.41presenilin enhancer, gamma-secretase subunit [Sour... [more]
AD000671.11.516e-1250.00novel protein[more]
PSENEN1.891e-1250.00presenilin enhancer, gamma-secretase subunit [Sour... [more]
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BLAST of Gamma-secretase subunit PEN-2 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
pen-27.717e-1536.36Gamma-secretase subunit pen-2 [Source:UniProtKB/S... [more]
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BLAST of Gamma-secretase subunit PEN-2 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2
Match NameE-valueIdentityDescription
pen-21.116e-2043.00gene:FBgn0053198 transcript:FBtr0340331[more]
pen-21.116e-2043.00gene:FBgn0053198 transcript:FBtr0089842[more]
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BLAST of Gamma-secretase subunit PEN-2 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
psenen1.338e-1941.41presenilin enhancer, gamma-secretase subunit [Sour... [more]
psenen1.338e-1941.41presenilin enhancer, gamma-secretase subunit [Sour... [more]
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BLAST of Gamma-secretase subunit PEN-2 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
psenen1.040e-1741.41presenilin enhancer, gamma-secretase subunit [Sour... [more]
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BLAST of Gamma-secretase subunit PEN-2 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 4
Match NameE-valueIdentityDescription
Psenen8.675e-2043.43presenilin enhancer gamma secretase subunit [Sourc... [more]
Psenen8.675e-2043.43presenilin enhancer gamma secretase subunit [Sourc... [more]
Psenen1.407e-1846.25presenilin enhancer gamma secretase subunit [Sourc... [more]
Psenen1.488e-751.22presenilin enhancer gamma secretase subunit [Sourc... [more]
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BLAST of Gamma-secretase subunit PEN-2 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q86BE9|PEN2_DROME1.119e-1943.00Gamma-secretase subunit pen-2 OS=Drosophila melano... [more]
sp|Q6QI68|PEN2_RAT3.718e-1944.44Gamma-secretase subunit PEN-2 OS=Rattus norvegicus... [more]
sp|Q9CQR7|PEN2_MOUSE6.068e-1943.43Gamma-secretase subunit PEN-2 OS=Mus musculus OX=1... [more]
sp|Q8JHF0|PEN2_DANRE1.010e-1841.41Gamma-secretase subunit PEN-2 OS=Danio rerio OX=79... [more]
sp|Q5G235|PEN2_BOVIN2.995e-1842.42Gamma-secretase subunit PEN-2 OS=Bos taurus OX=991... [more]
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BLAST of Gamma-secretase subunit PEN-2 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A3S0ZZ852.191e-2153.75Uncharacterized protein OS=Elysia chlorotica OX=18... [more]
A0A2C9JEK11.196e-2044.44Uncharacterized protein OS=Biomphalaria glabrata O... [more]
A0A1I8IIK86.033e-2055.00Uncharacterized protein OS=Macrostomum lignano OX=... [more]
F6VQU71.769e-1947.47Uncharacterized protein OS=Monodelphis domestica O... [more]
S4RAK42.520e-1942.42Presenilin enhancer, gamma-secretase subunit OS=Pe... [more]
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BLAST of Gamma-secretase subunit PEN-2 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
psenen4.718e-1940.40presenilin enhancer, gamma-secretase subunit [Sour... [more]
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BLAST of Gamma-secretase subunit PEN-2 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
psenen1.936e-2342.42presenilin enhancer, gamma-secretase subunit [Sour... [more]
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BLAST of Gamma-secretase subunit PEN-2 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Gamma-secretase subunit PEN-2 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO460975.104e-2042.42Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Gamma-secretase subunit PEN-2 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
psenen4.658e-1840.40presenilin enhancer, gamma-secretase subunit [Sour... [more]
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BLAST of Gamma-secretase subunit PEN-2 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 4
Match NameE-valueIdentityDescription
SMESG000035771.13.520e-64100.00SMESG000035771.1[more]
SMESG000015815.11.985e-1543.48SMESG000015815.1[more]
SMESG000015820.13.432e-1544.00SMESG000015820.1[more]
SMESG000015821.19.554e-1448.00SMESG000015821.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30029116 ID=SMED30029116|Name=Gamma-secretase subunit PEN-2|organism=Schmidtea mediterranea sexual|type=transcript|length=1247bp
CACGGGAGGAGTAAATTCAGATATTTTGGAAAATTCAACAGATAAGCCGC
TATTGTGTATAAATCGCTCCTGCACACTTACCGTATGTAAGTTTCCAGCA
ATTGGCTTGCCAGTATACCGGTATATGTATTTCACTAAAGCACAGCATCA
ATGCGCATTAGTCACAGTCGTTAAAAAAATTTACTTATCATATAAGTCTC
ACACGTCATTTAAAAAAAGGAAAAAAGTAGAGACTTATAGCCCGGAAAAT
ACGATATTTAGGATAAAATGTTTTTAAGAAAATTGTGTAGAAAATGCTTT
ATTCGGTTCTGTTACATGTAAACACTTAAATGGAGTCTATATACTGCTAG
ACTTGGACCTAGGAGTGGCCGCTGTTCTTCACATAGAAATACTTTTTTAC
ATTTATATTTGAATTAGCTACCTCATCTATTAGGCAAATAGACAGATATT
TTGACNTTTAATGAATTAAATATTTTAGTAGCATTTTTGAAATACTATTT
TTTCTAGATAAATTACCGATTTATAATAATATTTTATATCTGACTGTCAA
ATTTAATCAAAATTTTTATTAATCTAATTTATGGATATTAACTCCGTTAG
TGGATCTGAAAAGTTATCAATATGCAAGAAATACTTTTATGGTGGTATAT
TCTGCCTTCCATTTATGTGGCTAATAAATTCTCTTTGGTTTTTTAAACTT
GCTTTTCAAGCCGAACAGTTTCCCGAACAAGGTCAAATTAAGCGATATGT
TATCGGAAGCATGATTGGAACAACATGTTGGACAGTGGCAATTTTTACAT
GGATAATTAAATATCAAATGGTTAGTCGGCTCATGCAAATTTCGTCGTTG
ACATTTAATGTTCCGCTCGGTGGTTGATGAAGATAGGAATTTATTTGTTT
ATATTTCTCAAATTATTCGTTATGTTATACTCGTGTGTTTTTTGATTTTT
TATTAATCATTTTTGCTTTGACTTTTTTGTGATCAACATTTATTAAATAA
CAATTAATTGCTTTGACAATGAATAATTGAAATTTTGGGAGTTGCTAATA
TTGTTGTTAATAAATTGACCTGTACCAAGTTATTGTTCCATTCCAAAGTG
ATTATAATTCTCCTTTCTTCCTTGGTTACGAAGTCAGGGGATTTGGAGAA
AACATTTTCAAAATATTCATCTCACTCGTTTTATTTGTCTGGTAAGATGG
ATAATTTTGTATTTTGAGGATTTCGTGTAATTTCTAGATTAATTCTA
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000070intestinal phagocyte
Vocabulary: INTERPRO
TermDefinition
IPR019379Gamma_Secretase_Asp_P_PEN2
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR019379Gamma-secretase aspartyl protease complex, presenilin enhancer-2 subunitPFAMPF10251PEN-2coord: 9..96
e-value: 6.3E-28
score: 97.0
IPR019379Gamma-secretase aspartyl protease complex, presenilin enhancer-2 subunitPANTHERPTHR16318GAMMA-SECRETASE SUBUNIT PEN-2coord: 5..80
NoneNo IPR availableTMHMMTMhelixcoord: 57..76
NoneNo IPR availableTMHMMTMhelixcoord: 20..42