Rhodanese-like protein

Overview
NameRhodanese-like protein
Smed IDSMED30029080
Length (bp)646
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Rhodanese-like protein (SMED30029080) t-SNE clustered cells

Violin plots show distribution of expression levels for Rhodanese-like protein (SMED30029080) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Rhodanese-like protein (SMED30029080) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Rhodanese-like protein (SMED30029080) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30029080

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30029080SMESG000079774.1 dd_Smed_v4_7388_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30029080SMESG000079774.1 dd_Smed_v4_7388_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30029080SMESG000079774.1 dd_Smed_v4_7388_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
reproductive organSMED30029080SMESG000079774.1 Contig50340newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30029080SMESG000079774.1 Contig50340uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Rhodanese-like protein vs. Ensembl Human
Match: TSTD1 (thiosulfate sulfurtransferase like domain containing 1 [Source:HGNC Symbol;Acc:HGNC:35410])

HSP 1 Score: 72.7886 bits (177), Expect = 3.857e-16
Identity = 32/109 (29.36%), Postives = 58/109 (53.21%), Query Frame = 2 
Query:  200 AYSVNSAQLHKIVLSEDPVIIDVREENEHRESKINGSINIPLSQLKAAFELPAEDFRKRFGVEKPDKTDDNLILHCRKGIRSFDGLKILHELGFTRSRHLDGGINDWFE 526
            A +V+  +L  ++ S    + DVR   E     I G++NIP+S+L++A ++    F+  +  EKP   D++L+  C+ G R     ++   LG+T +R+  G   +W E
Sbjct:    4 APTVSLPELRSLLASGRARLFDVRSREEAAAGTIPGALNIPVSELESALQMEPAAFQALYSAEKPKLEDEHLVFFCQMGKRGLQATQLARSLGYTGARNYAGAYREWLE 112
[Close]
BLAST of Rhodanese-like protein vs. Ensembl Human
Match: TSTD1 (thiosulfate sulfurtransferase like domain containing 1 [Source:HGNC Symbol;Acc:HGNC:35410])

HSP 1 Score: 72.7886 bits (177), Expect = 5.213e-16
Identity = 32/109 (29.36%), Postives = 58/109 (53.21%), Query Frame = 2 
Query:  200 AYSVNSAQLHKIVLSEDPVIIDVREENEHRESKINGSINIPLSQLKAAFELPAEDFRKRFGVEKPDKTDDNLILHCRKGIRSFDGLKILHELGFTRSRHLDGGINDWFE 526
            A +V+  +L  ++ S    + DVR   E     I G++NIP+S+L++A ++    F+  +  EKP   D++L+  C+ G R     ++   LG+T +R+  G   +W E
Sbjct:   11 APTVSLPELRSLLASGRARLFDVRSREEAAAGTIPGALNIPVSELESALQMEPAAFQALYSAEKPKLEDEHLVFFCQMGKRGLQATQLARSLGYTGARNYAGAYREWLE 119
[Close]
BLAST of Rhodanese-like protein vs. Ensembl Human
Match: TSTD1 (thiosulfate sulfurtransferase like domain containing 1 [Source:HGNC Symbol;Acc:HGNC:35410])

HSP 1 Score: 61.2326 bits (147), Expect = 7.522e-12
Identity = 28/95 (29.47%), Postives = 51/95 (53.68%), Query Frame = 2 
Query:  200 AYSVNSAQLHKIVLSEDPVIIDVREENEHRESKINGSINIPLSQLKAAFELPAEDFRKRFGVEKPDKTDDNLILHCRKGIRSFDGLKILHELGFT 484
            A +V+  +L  ++ S    + DVR   E     I G++NIP+S+L++A ++    F+  +  EKP   D++L+  C+ G R     ++   LG+T
Sbjct:    4 APTVSLPELRSLLASGRARLFDVRSREEAAAGTIPGALNIPVSELESALQMEPAAFQALYSAEKPKLEDEHLVFFCQMGKRGLQATQLARSLGYT 98
[Close]
BLAST of Rhodanese-like protein vs. Ensembl Human
Match: TSTD1 (thiosulfate sulfurtransferase like domain containing 1 [Source:HGNC Symbol;Acc:HGNC:35410])

HSP 1 Score: 50.447 bits (119), Expect = 3.798e-8
Identity = 19/68 (27.94%), Postives = 37/68 (54.41%), Query Frame = 2 
Query:  323 LSQLKAAFELPAEDFRKRFGVEKPDKTDDNLILHCRKGIRSFDGLKILHELGFTRSRHLDGGINDWFE 526
            +S+L++A ++    F+  +  EKP   D++L+  C+ G R     ++   LG+T +R+  G   +W E
Sbjct:    4 VSELESALQMEPAAFQALYSAEKPKLEDEHLVFFCQMGKRGLQATQLARSLGYTGARNYAGAYREWLE 71
[Close]
BLAST of Rhodanese-like protein vs. Ensembl Fly
Match: CG12279 (gene:FBgn0038080 transcript:FBtr0082656)

HSP 1 Score: 62.3882 bits (150), Expect = 1.253e-12
Identity = 35/89 (39.33%), Postives = 50/89 (56.18%), Query Frame = 2 
Query:  257 IIDVREENEHRESKI-NGSINIPLSQLKAAFELPAEDFRKRFGVEKPDKTDDNLILHCRKGIRSFDGLKILHELGFTRSRHLDGGINDW 520
            + DVR E+E +E+ +   SINIPLS+L+ A  LP EDF + +G  KP   D  LI  C+ G R+     +   LGFT ++   G   +W
Sbjct:   18 LFDVRNESELKETGVLPASINIPLSELEKALNLPEEDFAQTYGRVKP-AVDAVLIFSCKAGGRAARAANLASTLGFTNAKAYAGSWTEW 105
[Close]
BLAST of Rhodanese-like protein vs. Ensembl Fly
Match: CG6000 (gene:FBgn0039145 transcript:FBtr0334920)

HSP 1 Score: 59.6918 bits (143), Expect = 3.598e-11
Identity = 39/126 (30.95%), Postives = 60/126 (47.62%), Query Frame = 2 
Query:  152 NTQRAFLSILPDEVLKAYSVNSAQLHKIVLSEDPVIIDVREENEHRES-KINGSINIPLSQLKAAFELPAEDFRKRFGVEKPDKTDDNLILHCRKGIRSFDGLKILHELGFTRSRHLDGGINDWFE 526
            NTQ   L     +  +   V+   + K+      ++IDVRE  E +E+ +I  SINIPL  +        + F+ ++G EKP K +  +I HC+ G RS    +    LGF   ++  G   DW E
Sbjct:   24 NTQSNLLQRFYSQAPQIGIVDYDVVKKLPSEPQKLLIDVREPEELKETGQIPASINIPLGVVSQELAASEQLFKSKYGREKP-KPETEIIFHCKIGKRSLKAAEAAAALGFKNVKNYQGSWLDWAE 148
[Close]
BLAST of Rhodanese-like protein vs. Ensembl Fly
Match: CG6000 (gene:FBgn0039145 transcript:FBtr0343101)

HSP 1 Score: 59.6918 bits (143), Expect = 3.598e-11
Identity = 39/126 (30.95%), Postives = 60/126 (47.62%), Query Frame = 2 
Query:  152 NTQRAFLSILPDEVLKAYSVNSAQLHKIVLSEDPVIIDVREENEHRES-KINGSINIPLSQLKAAFELPAEDFRKRFGVEKPDKTDDNLILHCRKGIRSFDGLKILHELGFTRSRHLDGGINDWFE 526
            NTQ   L     +  +   V+   + K+      ++IDVRE  E +E+ +I  SINIPL  +        + F+ ++G EKP K +  +I HC+ G RS    +    LGF   ++  G   DW E
Sbjct:   24 NTQSNLLQRFYSQAPQIGIVDYDVVKKLPSEPQKLLIDVREPEELKETGQIPASINIPLGVVSQELAASEQLFKSKYGREKP-KPETEIIFHCKIGKRSLKAAEAAAALGFKNVKNYQGSWLDWAE 148
[Close]
BLAST of Rhodanese-like protein vs. Ensembl Fly
Match: CG4456 (gene:FBgn0001228 transcript:FBtr0100655)

HSP 1 Score: 56.225 bits (134), Expect = 2.713e-10
Identity = 32/94 (34.04%), Postives = 48/94 (51.06%), Query Frame = 2 
Query:  248 DPVIIDVREENEHRESK-INGSINIPLSQLKAAFELPAEDFRKRFGVEKPDKTDDNLILHCRKGIRSFDGLKILHELGFTRSRHLDGGINDWFE 526
            D  +IDVR + E +++  I  SINIPL +L  A  L    F+ ++G  KP+K    +I  CR G R  +  KI    G++      G  N+W +
Sbjct:   15 DVYLIDVRRKEELQQTGFIPASINIPLDELDKALNLDGSAFKNKYGRSKPEKQSP-IIFTCRSGNRVLEAEKIAKSQGYSNVVIYKGSWNEWAQ 107
[Close]
BLAST of Rhodanese-like protein vs. Ensembl Fly
Match: CG4456 (gene:FBgn0001228 transcript:FBtr0303474)

HSP 1 Score: 56.225 bits (134), Expect = 2.713e-10
Identity = 32/94 (34.04%), Postives = 48/94 (51.06%), Query Frame = 2 
Query:  248 DPVIIDVREENEHRESK-INGSINIPLSQLKAAFELPAEDFRKRFGVEKPDKTDDNLILHCRKGIRSFDGLKILHELGFTRSRHLDGGINDWFE 526
            D  +IDVR + E +++  I  SINIPL +L  A  L    F+ ++G  KP+K    +I  CR G R  +  KI    G++      G  N+W +
Sbjct:   15 DVYLIDVRRKEELQQTGFIPASINIPLDELDKALNLDGSAFKNKYGRSKPEKQSP-IIFTCRSGNRVLEAEKIAKSQGYSNVVIYKGSWNEWAQ 107
[Close]
BLAST of Rhodanese-like protein vs. Ensembl Zebrafish
Match: si:ch211-161h7.8 (si:ch211-161h7.8 [Source:ZFIN;Acc:ZDB-GENE-091204-302])

HSP 1 Score: 97.4413 bits (241), Expect = 4.336e-25
Identity = 43/106 (40.57%), Postives = 65/106 (61.32%), Query Frame = 2 
Query:  209 VNSAQLHKIVLSEDPVIIDVREENEHRESKINGSINIPLSQLKAAFELPAEDFRKRFGVEKPDKTDDNLILHCRKGIRSFDGLKILHELGFTRSRHLDGGINDWFE 526
            V   QL  ++ +    + DVR  +E +  +I  S+N+PL +L+ + +LPAE F ++F V+ P K DDN++ HCR G RS   L+  H LGF+++RH  GG  DW E
Sbjct:   71 VTYEQLKGMLANHSVQLFDVRNPDEFQAGRIPDSVNVPLGELEVSLKLPAEKFEEQFKVKAPQKADDNIVFHCRSGKRSLTALETAHRLGFSKARHYAGGYIDWEE 176
[Close]
BLAST of Rhodanese-like protein vs. Ensembl Zebrafish
Match: CABZ01058650.1 (thiosulfate sulfurtransferase/rhodanese-like domain-containing protein 1 [Source:NCBI gene;Acc:561325])

HSP 1 Score: 87.8113 bits (216), Expect = 1.488e-21
Identity = 41/107 (38.32%), Postives = 67/107 (62.62%), Query Frame = 2 
Query:  206 SVNSAQLHKIVLSEDPVIIDVREENEHRESKINGSINIPLSQLKAAFELPAEDFRKRFGVEKPDKTDDNLILHCRKGIRSFDGLKILHELGFTRSRHLDGGINDWFE 526
            +V   QL  ++ +    +IDVRE +E     I G+ NIPL +L+ A  L  + FR+R+GV KP   D +++L+C++G+RS + L+I   L ++R+RH  GG ++W E
Sbjct:   48 AVTYEQLKHMMSTGRVQLIDVREPDELTAGFIPGATNIPLGELEEALTLDPDQFRQRYGVSKPHPDDSDIVLYCQRGVRSLNALEIAIRLEYSRARHYVGGYSEWAE 154
[Close]
BLAST of Rhodanese-like protein vs. Ensembl Zebrafish
Match: si:dkey-165n16.1 (si:dkey-165n16.1 [Source:ZFIN;Acc:ZDB-GENE-100922-224])

HSP 1 Score: 86.6557 bits (213), Expect = 6.051e-21
Identity = 42/107 (39.25%), Postives = 66/107 (61.68%), Query Frame = 2 
Query:  209 VNSAQLHKIVLSEDPVIIDVREENEHRE-SKINGSINIPLSQLKAAFELPAEDFRKRFGVEKPDKTDDNLILHCRKGIRSFDGLKILHELGFTRSRHLDGGINDWFE 526
            V+  QL K+++S   V+IDVRE  E RE   I GSIN+PL Q+  A +L  ++F++++G + P K+  N++  C  G+RS   L+    LG+T+ +H  GG  +W E
Sbjct:   66 VSYEQLKKLLVSGSSVVIDVREPWELREYGNIQGSINVPLGQVNGALQLTPDEFKEKYGGDMPSKS-QNIVFSCLAGVRSKHALEAAVSLGYTKVQHFPGGWQEWAE 171
[Close]
BLAST of Rhodanese-like protein vs. Ensembl Zebrafish
Match: BX537102.2 (si:dkey-34l15.1 [Source:NCBI gene;Acc:563265])

HSP 1 Score: 62.003 bits (149), Expect = 3.329e-12
Identity = 33/110 (30.00%), Postives = 52/110 (47.27%), Query Frame = 2 
Query:  209 VNSAQLHKIVLSEDPVIIDVREENEHRESKINGSINIPLSQLKAAFELPAEDFRKRFGVEKPDKTDDNLILHCRKGIRSFDGLKILHELGFTRSRHLDGGINDWFEYKTK 538
            ++ + L  I+     V++DVR   E     I GSI+IP+  +     L A +F+ +FGV KP      L+  C+ G R     +    LGF  +R+  G   +W E + K
Sbjct:    8 ISYSDLKSILAKGSAVLVDVRTNEEVARGTIPGSIHIPVGNIGQDLSLDAAEFQSKFGVGKPPLDCTELVFFCQLGRRGAAATEKAKSLGFKNARNYAGAYKEWSEKEGK 117
[Close]
BLAST of Rhodanese-like protein vs. Ensembl Zebrafish
Match: TSTD1 (si:dkey-34l15.1 [Source:ZFIN;Acc:ZDB-GENE-081107-63])

HSP 1 Score: 60.077 bits (144), Expect = 1.818e-11
Identity = 32/110 (29.09%), Postives = 52/110 (47.27%), Query Frame = 2 
Query:  209 VNSAQLHKIVLSEDPVIIDVREENEHRESKINGSINIPLSQLKAAFELPAEDFRKRFGVEKPDKTDDNLILHCRKGIRSFDGLKILHELGFTRSRHLDGGINDWFEYKTK 538
            ++ + L  ++     V++DVR   E     I GSI+IP+  +     L A +F+ +FGV KP      L+  C+ G R     +    LGF  +R+  G   +W E + K
Sbjct:    8 ISYSDLKSLLAKGSVVLVDVRTNEEVARGTIPGSIHIPVGNVGQDLSLDAAEFQSKFGVGKPPLDCTELVFFCQLGRRGAAATEKAKSLGFKNARNYAGAYKEWSEKEGK 117
[Close]
BLAST of Rhodanese-like protein vs. Ensembl Xenopus
Match: ENSXETT00000042802.1 (thiosulfate sulfurtransferase (rhodanese)-like domain containing 3 [Source:NCBI gene;Acc:100486879])

HSP 1 Score: 77.411 bits (189), Expect = 2.563e-17
Identity = 48/132 (36.36%), Postives = 69/132 (52.27%), Query Frame = 2 
Query:  152 NTQRAFLSILPDEVLKAYSVNSAQLHKIVLSEDPVIIDVREENEHRE-SKINGSINIPLSQLKAAFELPAEDFRKRFGVEKPDKTDDNLILHCRKGIRSFDGLKILHELGFTRSRHLDGGINDWFEYKTKSK 544
            +  R F S+ P + +K       +L  ++  E  VIIDVRE  E +E   I GS++IP+  L +A +L    F K++ V+ P+KT   LI  C  GIRS   + +   LGF R  H  GG  DW  ++T  K
Sbjct:   40 SVARGFSSV-PSQTIKY-----EELKDLLKEEGVVIIDVREPWEVKEYGVIKGSLSIPMGDLASALQLDPLTFEKKYQVKMPEKTS-TLIFSCLAGIRSKKAVDVAGSLGFKRVHHYAGGFEDWARHETPEK 164
[Close]
BLAST of Rhodanese-like protein vs. Ensembl Mouse
Match: Tstd1 (thiosulfate sulfurtransferase (rhodanese)-like domain containing 1 [Source:MGI Symbol;Acc:MGI:3648482])

HSP 1 Score: 72.7886 bits (177), Expect = 3.873e-16
Identity = 34/107 (31.78%), Postives = 55/107 (51.40%), Query Frame = 2 
Query:  206 SVNSAQLHKIVLSEDPVIIDVREENEHRESKINGSINIPLSQLKAAFELPAEDFRKRFGVEKPDKTDDNLILHCRKGIRSFDGLKILHELGFTRSRHLDGGINDWFE 526
            +V+ ++L  +V S    + DVR   E     I G++NIP+S+L+ A  +    F+  +  EKP   D NLI  C+ G R     ++   LG+T +R+  G   +W E
Sbjct:   24 TVSFSELRSLVASGRARLFDVRSPEEAAAGTIPGALNIPVSELEIALNMDPAAFQTLYCAEKPKLEDKNLIFFCQMGRRGLQATQLAQGLGYTGARNYAGAYKEWLE 130
[Close]
BLAST of Rhodanese-like protein vs. Ensembl Mouse
Match: Tstd3 (thiosulfate sulfurtransferase (rhodanese)-like domain containing 3 [Source:MGI Symbol;Acc:MGI:1924282])

HSP 1 Score: 71.633 bits (174), Expect = 1.994e-15
Identity = 39/109 (35.78%), Postives = 61/109 (55.96%), Query Frame = 2 
Query:  209 VNSAQLHKIVLSEDPVIIDVREENEHRE-SKINGSINIPLSQLKAAFELPAEDFRKRFGVEKPDKTDDNLILHCRKGIRSFDGLKILHELGFTRSRHLDGGINDWFEYK 532
            V   +L  ++ S+D ++IDVR   E  E  KI GSINIPL ++  A ++   DF++++   KP K+ D L+  C  G+RS   +     LGF  ++H  GG  +W  Y+
Sbjct:   42 VTYRELKSLLNSKDIMLIDVRNTLEILEQGKIPGSINIPLDEVGEALQMNPVDFKEKYCQVKPSKS-DRLVFSCLAGVRSKKAMDTAISLGFNSAQHYAGGWKEWVTYE 149
[Close]
BLAST of Rhodanese-like protein vs. UniProt/SwissProt
Match: sp|Q8NFU3|TSTD1_HUMAN (Thiosulfate:glutathione sulfurtransferase OS=Homo sapiens OX=9606 GN=TSTD1 PE=1 SV=3)

HSP 1 Score: 72.7886 bits (177), Expect = 1.852e-15
Identity = 32/109 (29.36%), Postives = 58/109 (53.21%), Query Frame = 2 
Query:  200 AYSVNSAQLHKIVLSEDPVIIDVREENEHRESKINGSINIPLSQLKAAFELPAEDFRKRFGVEKPDKTDDNLILHCRKGIRSFDGLKILHELGFTRSRHLDGGINDWFE 526
            A +V+  +L  ++ S    + DVR   E     I G++NIP+S+L++A ++    F+  +  EKP   D++L+  C+ G R     ++   LG+T +R+  G   +W E
Sbjct:    4 APTVSLPELRSLLASGRARLFDVRSREEAAAGTIPGALNIPVSELESALQMEPAAFQALYSAEKPKLEDEHLVFFCQMGKRGLQATQLARSLGYTGARNYAGAYREWLE 112
[Close]
BLAST of Rhodanese-like protein vs. UniProt/SwissProt
Match: sp|Q9D0B5|TSTD3_MOUSE (Thiosulfate sulfurtransferase/rhodanese-like domain-containing protein 3 OS=Mus musculus OX=10090 GN=Tstd3 PE=1 SV=1)

HSP 1 Score: 71.633 bits (174), Expect = 1.395e-14
Identity = 39/109 (35.78%), Postives = 61/109 (55.96%), Query Frame = 2 
Query:  209 VNSAQLHKIVLSEDPVIIDVREENEHRE-SKINGSINIPLSQLKAAFELPAEDFRKRFGVEKPDKTDDNLILHCRKGIRSFDGLKILHELGFTRSRHLDGGINDWFEYK 532
            V   +L  ++ S+D ++IDVR   E  E  KI GSINIPL ++  A ++   DF++++   KP K+ D L+  C  G+RS   +     LGF  ++H  GG  +W  Y+
Sbjct:   42 VTYRELKSLLNSKDIMLIDVRNTLEILEQGKIPGSINIPLDEVGEALQMNPVDFKEKYCQVKPSKS-DRLVFSCLAGVRSKKAMDTAISLGFNSAQHYAGGWKEWVTYE 149
[Close]
BLAST of Rhodanese-like protein vs. UniProt/SwissProt
Match: sp|Q10215|RDL_SCHPO (Putative thiosulfate sulfurtransferase, mitochondrial OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC4H3.07c PE=3 SV=2)

HSP 1 Score: 67.781 bits (164), Expect = 3.219e-13
Identity = 34/97 (35.05%), Postives = 57/97 (58.76%), Query Frame = 2 
Query:  254 VIIDVREENEHRESKINGSINIPLSQLKAAFELPAEDFRKRFGVEKPDKTDDNLILHCRKGIRSFDGLKILHELGFTRSRHLDGGINDWFEYKTKSK 544
            V+IDVRE +E ++  I  S N+P+ +++ A +L  E+F K +G  KP   +DN++++CR G RS     IL +LG+   +++      W E+  K K
Sbjct:   48 VLIDVREPDEFKQGAIETSYNLPVGKIEEAMKLSDEEFSKTYGFSKP-VFEDNVVVYCRSGRRSTTASDILTKLGY---KNIGNYTGSWLEWSDKIK 140
[Close]
BLAST of Rhodanese-like protein vs. UniProt/SwissProt
Match: sp|Q12305|RDL1_YEAST (Thiosulfate:glutathione sulfurtransferase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=RDL1 PE=1 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 1.200e-12
Identity = 35/104 (33.65%), Postives = 54/104 (51.92%), Query Frame = 2 
Query:  224 LHKIVLSEDP--VIIDVREENEHRESKINGSINIPLSQLKAAFELPAEDFRKRFGVEKPDKTDDNLILHCRKGIRSFDGLKILHELGFTRSRHLDGGINDWFEY 529
            + +IV   DP  V++DVRE +E+    I  SIN+P      AF L   +F K+ G+ KPD   + LI +C  G R  +  K+    G++ +    G +NDW  +
Sbjct:   30 MKRIVGKHDPNVVLVDVREPSEYSIVHIPASINVPYRSHPDAFALDPLEFEKQIGIPKPDSAKE-LIFYCASGKRGGEAQKVASSHGYSNTSLYPGSMNDWVSH 132
[Close]
BLAST of Rhodanese-like protein vs. UniProt/SwissProt
Match: sp|Q08742|RDL2_YEAST (Thiosulfate sulfurtransferase RDL2, mitochondrial OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=RDL2 PE=1 SV=1)

HSP 1 Score: 60.4622 bits (145), Expect = 1.925e-10
Identity = 35/122 (28.69%), Postives = 60/122 (49.18%), Query Frame = 2 
Query:  182 PDEVLKAYSVNSAQLHKI----VLSEDP----VIIDVREENEHRESKINGSINIPLSQLKAAFELPAEDFRKRFGVEKPDKTDDNLILHCRKGIRSFDGLKILHELGFTRSRHLDGGINDWF 523
            P  VL+ ++  + +++       L E P    +++DVRE  E ++ K+  +INIP++    A  LP ++F K F   KP   D  LI  C KG+R+    ++    G+  +    G I +W 
Sbjct:   18 PTLVLRTFTTKAPKIYTFDQVRNLVEHPNDKKLLVDVREPKEVKDYKMPTTINIPVNSAPGALGLPEKEFHKVFQFAKPPH-DKELIFLCAKGVRAKTAEELARSYGYENTGIYPGSITEWL 138
[Close]
BLAST of Rhodanese-like protein vs. TrEMBL
Match: A0A3P9HXG0 (Si:ch211-161h7.8 OS=Oryzias latipes OX=8090 PE=4 SV=1)

HSP 1 Score: 107.457 bits (267), Expect = 1.258e-25
Identity = 46/114 (40.35%), Postives = 74/114 (64.91%), Query Frame = 2 
Query:  197 KAYSVNSAQLHKIVLSEDPVIIDVREENEHRESKINGSINIPLSQLKAAFELPAEDFRKRFGVEKPDKTDDNLILHCRKGIRSFDGLKILHELGFTRSRHLDGGINDWFEYKTK 538
            +A+ V  +QL  ++ + D  + DVR+ +EH+E +I  ++N+PL  L+ +  LP++ F +RF V+ P+K D N++ HCR G RSF  L I H+LGF ++RH  GG  +W E + K
Sbjct:   63 QAFVVTHSQLKTMLTNRDIQLFDVRDPDEHQEGRIPQAVNLPLGVLEESLMLPSDHFEQRFKVKAPEKEDANIVFHCRTGKRSFQALGIAHQLGFHKARHYKGGYAEWVEQEGK 176
[Close]
BLAST of Rhodanese-like protein vs. TrEMBL
Match: A0A437C7V2 (Rhodanese domain-containing protein OS=Oryzias javanicus OX=123683 GN=OJAV_G00197630 PE=4 SV=1)

HSP 1 Score: 105.531 bits (262), Expect = 5.772e-25
Identity = 46/114 (40.35%), Postives = 74/114 (64.91%), Query Frame = 2 
Query:  197 KAYSVNSAQLHKIVLSEDPVIIDVREENEHRESKINGSINIPLSQLKAAFELPAEDFRKRFGVEKPDKTDDNLILHCRKGIRSFDGLKILHELGFTRSRHLDGGINDWFEYKTK 538
            +A  V  +QL  ++ + D  + DVR+ +EH+E +I  +IN+PL  L+ + +LP + F ++F V+ P+K D N++ HCR G RSF  L I H+LGF ++RH  GG ++W E + K
Sbjct:   59 EASVVTHSQLKTMLTNRDIQLFDVRDPDEHQEGRIPQAINLPLGVLEESLQLPPDHFEQKFKVKAPEKEDGNIVFHCRSGKRSFKALGIAHQLGFHKARHYKGGFSEWVEREGK 172
[Close]
BLAST of Rhodanese-like protein vs. TrEMBL
Match: H2LZK5 (Si:ch211-161h7.8 OS=Oryzias latipes OX=8090 GN=LOC101163444 PE=4 SV=1)

HSP 1 Score: 105.531 bits (262), Expect = 6.246e-25
Identity = 45/114 (39.47%), Postives = 75/114 (65.79%), Query Frame = 2 
Query:  197 KAYSVNSAQLHKIVLSEDPVIIDVREENEHRESKINGSINIPLSQLKAAFELPAEDFRKRFGVEKPDKTDDNLILHCRKGIRSFDGLKILHELGFTRSRHLDGGINDWFEYKTK 538
            +A+ V  +QL  ++ + D  + DVR+ +EH+E +I  ++N+PL  L+ + +LP++ F +RF V+ P+K D N++ HCR G RSF  L I H+LGF ++RH  GG  +W + + K
Sbjct:   62 QAFVVTHSQLKTMLTNRDIQLFDVRDPDEHQEGRIPQAVNLPLGVLEESLKLPSDHFEQRFKVKAPEKEDANIVFHCRTGKRSFQALGIAHQLGFHKARHYKGGYVEWVKQEGK 175
[Close]
BLAST of Rhodanese-like protein vs. TrEMBL
Match: A0A3B3BJ88 (Si:ch211-161h7.8 OS=Oryzias melastigma OX=30732 PE=4 SV=1)

HSP 1 Score: 104.76 bits (260), Expect = 1.209e-24
Identity = 45/110 (40.91%), Postives = 71/110 (64.55%), Query Frame = 2 
Query:  197 KAYSVNSAQLHKIVLSEDPVIIDVREENEHRESKINGSINIPLSQLKAAFELPAEDFRKRFGVEKPDKTDDNLILHCRKGIRSFDGLKILHELGFTRSRHLDGGINDWFE 526
            +A  V  +QL  ++ + D  + DVR+ +EH+E +I  ++N+PL  L+ + +LP + F +RF V+ P+K D N++ HCR G RSF  L I H+LGF ++RH  GG + W E
Sbjct:   59 EASVVTHSQLKTMLTNRDIQLFDVRDPDEHQEGRIPQAVNLPLGVLEESLQLPPDHFEQRFKVKAPEKEDGNIVFHCRSGKRSFKALGIAHQLGFHKARHYKGGYSQWVE 168
[Close]
BLAST of Rhodanese-like protein vs. TrEMBL
Match: A0A3B3BK68 (Si:ch211-161h7.8 OS=Oryzias melastigma OX=30732 PE=4 SV=1)

HSP 1 Score: 104.76 bits (260), Expect = 2.355e-24
Identity = 45/110 (40.91%), Postives = 71/110 (64.55%), Query Frame = 2 
Query:  197 KAYSVNSAQLHKIVLSEDPVIIDVREENEHRESKINGSINIPLSQLKAAFELPAEDFRKRFGVEKPDKTDDNLILHCRKGIRSFDGLKILHELGFTRSRHLDGGINDWFE 526
            +A  V  +QL  ++ + D  + DVR+ +EH+E +I  ++N+PL  L+ + +LP + F +RF V+ P+K D N++ HCR G RSF  L I H+LGF ++RH  GG + W E
Sbjct:   77 EASVVTHSQLKTMLTNRDIQLFDVRDPDEHQEGRIPQAVNLPLGVLEESLQLPPDHFEQRFKVKAPEKEDGNIVFHCRSGKRSFKALGIAHQLGFHKARHYKGGYSQWVE 186
[Close]
BLAST of Rhodanese-like protein vs. Ensembl Cavefish
Match: si:ch211-161h7.8 (thiosulfate sulfurtransferase/rhodanese-like domain-containing protein 1 [Source:NCBI gene;Acc:103025070])

HSP 1 Score: 98.5969 bits (244), Expect = 7.963e-26
Identity = 47/119 (39.50%), Postives = 69/119 (57.98%), Query Frame = 2 
Query:  170 LSILPDEVLKAYSVNSAQLHKIVLSEDPVIIDVREENEHRESKINGSINIPLSQLKAAFELPAEDFRKRFGVEKPDKTDDNLILHCRKGIRSFDGLKILHELGFTRSRHLDGGINDWFE 526
            +S  PD     Y V   QL  ++ S    + D+R  +E +   I GS+NIPL QL+ + +L ++ F K F V+ P K DDN++ HC++G RS   L I   LGF+R+RH  GG ++W E
Sbjct:   37 VSFNPDPSNPDYVVTREQLKAMLASHSVQLFDIRNRDEFQAGHIAGSVNIPLDQLEESLKLSSKTFEKCFKVKAPKKEDDNIVFHCQRGRRSLTALDIAWRLGFSRARHYAGGYSEWAE 155
[Close]
BLAST of Rhodanese-like protein vs. Ensembl Cavefish
Match: ENSAMXT00000037356.1 (thiosulfate sulfurtransferase/rhodanese-like domain-containing protein 3 [Source:NCBI gene;Acc:103030362])

HSP 1 Score: 81.2629 bits (199), Expect = 5.419e-19
Identity = 37/105 (35.24%), Postives = 67/105 (63.81%), Query Frame = 2 
Query:  221 QLHKIVLSEDPVIIDVREENEHRE-SKINGSINIPLSQLKAAFELPAEDFRKRFGVEKPDKTDDNLILHCRKGIRSFDGLKILHELGFTRSRHLDGGINDWFEYK 532
            +L K+++++  V+IDVRE  E RE   I  +IN+PL Q+ +A +L  E+F++++G + P  T +N++  C  G+RS   L+    LG++ ++H  GG  DW +++
Sbjct:   66 ELKKLLVAKSGVVIDVREPWELREYGNIPSAINVPLGQVTSALQLEPEEFKEKYGGDMPS-TTENVVFTCLAGMRSIKALQAATSLGYSNAQHYLGGWQDWAKHE 169
[Close]
BLAST of Rhodanese-like protein vs. Ensembl Cavefish
Match: si:dkey-34l15.1 (thiosulfate sulfurtransferase/rhodanese-like domain-containing protein 1 [Source:NCBI gene;Acc:103030441])

HSP 1 Score: 66.2402 bits (160), Expect = 9.804e-14
Identity = 34/95 (35.79%), Postives = 52/95 (54.74%), Query Frame = 2 
Query:  254 VIIDVREENEHRESKINGSINIPLSQLKAAFELPAEDFRKRFGVEKPDKTDDNLILHCRKGIRSFDGLKILHELGFTRSRHLDGGINDWFEYKTK 538
            V++DVR ++E  + +I GSI+IPLS ++    L  + F+ +FGV KP     NL+ HC+ G R          LGF  +R+  G   +W E + K
Sbjct:   41 VLVDVRNKDEVDKGRIPGSIHIPLSDVERDLSLDPDAFQTKFGVAKPPLDTPNLVFHCQLGRRGQAATDKAKSLGFENARNYVGAYKEWSEKEGK 135
[Close]
BLAST of Rhodanese-like protein vs. Ensembl Cavefish
Match: si:dkey-34l15.1 (thiosulfate sulfurtransferase/rhodanese-like domain-containing protein 1 [Source:NCBI gene;Acc:103030441])

HSP 1 Score: 57.3806 bits (137), Expect = 1.644e-10
Identity = 29/78 (37.18%), Postives = 44/78 (56.41%), Query Frame = 2 
Query:  254 VIIDVREENEHRESKINGSINIPLSQLKAAFELPAEDFRKRFGVEKPDKTDDNLILHCRKGIRSFDGLKILHELGFTR 487
            V++DVR ++E  + +I GSI+IPLS ++    L  + F+ +FGV KP     NL+ HC+ G R          LGF +
Sbjct:   41 VLVDVRNKDEVDKGRIPGSIHIPLSDVERDLSLDPDAFQTKFGVAKPPLDTPNLVFHCQLGRRGQAATDKAKSLGFEK 118
[Close]
BLAST of Rhodanese-like protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000006076.1 (pep scaffold:Pmarinus_7.0:GL476493:725894:734457:1 gene:ENSPMAG00000005492.1 transcript:ENSPMAT00000006076.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 77.7962 bits (190), Expect = 8.970e-19
Identity = 40/112 (35.71%), Postives = 67/112 (59.82%), Query Frame = 2 
Query:  209 VNSAQLHKIVLSEDPVIIDVREENEHRESKINGSINIPLSQLKAAFELPAEDFRKRFGVEKPDKTDDNLILHCRKGIRSFDGLKILHELGFTRSRHLDGGINDWFEYKTKSK 544
            V+  +L +++ + D  + DVRE  E  E  I  +INIP+ ++  AF   +  F+  +GV+ P K +DN++++CR G+R    L  L  LG+T+SR+  GG  +W   K+K+K
Sbjct:   28 VSYEELKRLLQNRDVSVFDVREPCETEEGMIPSAINIPVGKINEAFIRDSGTFKGLYGVDMPHK-EDNIVVYCRSGMRGQTALYSLRRLGYTKSRNYTGGYTEW---KSKAK 135
[Close]
BLAST of Rhodanese-like protein vs. Ensembl Sea Lamprey
Match: si:ch211-161h7.8 (si:ch211-161h7.8 [Source:ZFIN;Acc:ZDB-GENE-091204-302])

HSP 1 Score: 77.0258 bits (188), Expect = 1.201e-18
Identity = 36/89 (40.45%), Postives = 53/89 (59.55%), Query Frame = 2 
Query:  257 IIDVREENEHRE-SKINGSINIPLSQLKAAFELPAEDFRKRFGVEKPDKTDDNLILHCRKGIRSFDGLKILHELGFTRSRHLDGGINDW 520
            ++DVRE +E +E   I G +N+PL +L  A +L   +FR R+G +    +  N +  CR G RS + L I  +LG+TR+RH  GG  DW
Sbjct:   18 LVDVREPSELKENGAIEGCVNVPLGELGEALQLSPREFRARYGSDLVGPSAPNTVFLCRAGRRSKEALLIAQKLGYTRARHYGGGWLDW 106
[Close]
BLAST of Rhodanese-like protein vs. Ensembl Yeast
Match: RDL1 (Thiosulfate sulfurtransferase; contains a rhodanese-like domain; localized to the mitochondrial outer membrane; protein abundance increases in response to DNA replication stress; similar to the human TSTD gene [Source:SGD;Acc:S000005811])

HSP 1 Score: 66.2402 bits (160), Expect = 1.728e-14
Identity = 35/104 (33.65%), Postives = 54/104 (51.92%), Query Frame = 2 
Query:  224 LHKIVLSEDP--VIIDVREENEHRESKINGSINIPLSQLKAAFELPAEDFRKRFGVEKPDKTDDNLILHCRKGIRSFDGLKILHELGFTRSRHLDGGINDWFEY 529
            + +IV   DP  V++DVRE +E+    I  SIN+P      AF L   +F K+ G+ KPD   + LI +C  G R  +  K+    G++ +    G +NDW  +
Sbjct:   30 MKRIVGKHDPNVVLVDVREPSEYSIVHIPASINVPYRSHPDAFALDPLEFEKQIGIPKPDSAKE-LIFYCASGKRGGEAQKVASSHGYSNTSLYPGSMNDWVSH 132
[Close]
BLAST of Rhodanese-like protein vs. Ensembl Yeast
Match: RDL2 (Protein with rhodanese activity; contains a rhodanese-like domain similar to Rdl1p, Uba4p, Tum1p, and Ych1p; overexpression causes a cell cycle delay; null mutant displays elevated frequency of mitochondrial genome loss [Source:SGD;Acc:S000005812])

HSP 1 Score: 60.4622 bits (145), Expect = 2.772e-12
Identity = 35/122 (28.69%), Postives = 60/122 (49.18%), Query Frame = 2 
Query:  182 PDEVLKAYSVNSAQLHKI----VLSEDP----VIIDVREENEHRESKINGSINIPLSQLKAAFELPAEDFRKRFGVEKPDKTDDNLILHCRKGIRSFDGLKILHELGFTRSRHLDGGINDWF 523
            P  VL+ ++  + +++       L E P    +++DVRE  E ++ K+  +INIP++    A  LP ++F K F   KP   D  LI  C KG+R+    ++    G+  +    G I +W 
Sbjct:   18 PTLVLRTFTTKAPKIYTFDQVRNLVEHPNDKKLLVDVREPKEVKDYKMPTTINIPVNSAPGALGLPEKEFHKVFQFAKPPH-DKELIFLCAKGVRAKTAEELARSYGYENTGIYPGSITEWL 138
[Close]
BLAST of Rhodanese-like protein vs. Ensembl Nematostella
Match: EDO34076 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SQG5])

HSP 1 Score: 87.4261 bits (215), Expect = 2.461e-21
Identity = 46/140 (32.86%), Postives = 79/140 (56.43%), Query Frame = 2 
Query:  104 QSYSKKTILTPRFQYINTQRAFLSILPDEVLKAYSVNSAQLHKIVLSEDPVIIDVREENEHRES-KINGSINIPLSQLKAAFELPAEDFRKRFGVEKPDKTDDNLILHCRKGIRSFDGLKILHELGFTRSRHLDGGINDW 520
            Q ++    +T R Q    +R   S +P +  +   V+  +L  ++  +   + DVRE  E  ES KI  SINIPL Q+  AF +  ++F+ ++G +KP + D +++ HC+ GIRS   LK +  +GF +++H  GG ++W
Sbjct:   71 QRFTHMLGVTERGQCACAKRTITSTIPIKDPET-EVSIEELQLLIKQQAIQLFDVREPYELEESGKIPFSINIPLRQVPEAFTMKPKEFKLKYGAKKPKRKDTDIVFHCKAGIRSKTALKAVRSMGFRKAKHYPGGFDEW 209
[Close]
BLAST of Rhodanese-like protein vs. Ensembl Nematostella
Match: EDO26397 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7TC43])

HSP 1 Score: 82.8037 bits (203), Expect = 1.215e-20
Identity = 36/89 (40.45%), Postives = 57/89 (64.04%), Query Frame = 2 
Query:  257 IIDVREENEHRES-KINGSINIPLSQLKAAFELPAEDFRKRFGVEKPDKTDDNLILHCRKGIRSFDGLKILHELGFTRSRHLDGGINDW 520
            + DVRE  E  ES KI  SINIP  Q+  AF +  ++F+ ++G +KP + D +++ HC+ GIRS   LK +  +GF +++H  GG ++W
Sbjct:   17 LFDVREPYELEESGKIPFSINIPCEQVPEAFTMKPKEFKLKYGAKKPKRKDTDIVFHCKAGIRSKTALKAVRSMGFRKAKHYPGGFDEW 105
[Close]
BLAST of Rhodanese-like protein vs. Ensembl Nematostella
Match: EDO29885 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T2D3])

HSP 1 Score: 81.2629 bits (199), Expect = 4.589e-20
Identity = 40/106 (37.74%), Postives = 61/106 (57.55%), Query Frame = 2 
Query:  224 LHKIVLSEDPVIIDVREENEHRES-KINGSINIPLSQLKAAFELPAEDFRKRFGVEKPDKTDDNLILHCRKGIRSFDGLKILHELGFTRSRHLDGGINDWFEYKTK 538
            L +++   D  + DVR   E  E+ +I G++NIPLS + AAF LP   F + + V  P KTD+N++ HCR G+R+ + +  L  LGF  + +  GG  +W E   K
Sbjct:    6 LKELLSKADIQLFDVRNPEELLETGQIPGAVNIPLSDITAAFSLPPAIFEQIYDVPMPSKTDNNIVFHCRTGVRAQNAIDTLKNLGFKGTHNYTGGWVEWQEAMMK 111
[Close]
BLAST of Rhodanese-like protein vs. Ensembl Nematostella
Match: EDO34075 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SQG4])

HSP 1 Score: 80.4925 bits (197), Expect = 4.180e-19
Identity = 41/114 (35.96%), Postives = 65/114 (57.02%), Query Frame = 2 
Query:  224 LHKIVLSEDPVIIDVREENEHRES-KINGSINIPLSQLKAAFELPAEDFRKRFGVEKPDKTDDNLILHCRKGIRSFDGLKILHELGFTRSRHLDGGINDWFEYKTKSK*DSNLN 562
            L +++   D  + DVR   E  E+ +I G++NIPLS + AAF LP   F + + V  P KTD+N++ HC+ G+R+ + +  L  LGF  + +  GG   W E++   K D+  N
Sbjct:   66 LKELLSKADIQLFDVRNPEELLETGQIPGAVNIPLSDITAAFMLPPAIFEQIYDVPMPSKTDNNIVFHCKTGVRAQNAIDTLKNLGFKGTHNYTGG---WVEWQEAMKKDTESN 176
[Close]
BLAST of Rhodanese-like protein vs. Ensembl Nematostella
Match: EDO34074 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SQG3])

HSP 1 Score: 71.2478 bits (173), Expect = 1.550e-16
Identity = 34/88 (38.64%), Postives = 54/88 (61.36%), Query Frame = 2 
Query:  224 LHKIVLSEDPVIIDVREENEHRES-KINGSINIPLSQLKAAFELPAEDFRKRFGVEKPDKTDDNLILHCRKGIRSFDGLKILHELGFT 484
            + +++  ED  + DVR+  E  E+ +I G++NIPLS + +AF LP   F + + V  P KTD+N++ HCR G RS   +  L  +G+T
Sbjct:    1 MKELLSKEDIQLFDVRDPEELLETGQIPGAVNIPLSDITSAFSLPPNIFEQIYDVPMPSKTDNNIVFHCRTGRRSQQAIDTLQNIGYT 88
[Close]
BLAST of Rhodanese-like protein vs. Ensembl Medaka
Match: si:ch211-161h7.8 (thiosulfate:glutathione sulfurtransferase [Source:NCBI gene;Acc:101163444])

HSP 1 Score: 105.531 bits (262), Expect = 2.351e-28
Identity = 45/114 (39.47%), Postives = 75/114 (65.79%), Query Frame = 2 
Query:  197 KAYSVNSAQLHKIVLSEDPVIIDVREENEHRESKINGSINIPLSQLKAAFELPAEDFRKRFGVEKPDKTDDNLILHCRKGIRSFDGLKILHELGFTRSRHLDGGINDWFEYKTK 538
            +A+ V  +QL  ++ + D  + DVR+ +EH+E +I  ++N+PL  L+ + +LP++ F +RF V+ P+K D N++ HCR G RSF  L I H+LGF ++RH  GG  +W + + K
Sbjct:   62 QAFVVTHSQLKTMLTNRDIQLFDVRDPDEHQEGRIPQAVNLPLGVLEESLKLPSDHFEQRFKVKAPEKEDANIVFHCRTGKRSFQALGIAHQLGFHKARHYKGGYVEWVKQEGK 175
[Close]
BLAST of Rhodanese-like protein vs. Ensembl Medaka
Match: si:ch211-161h7.8 (thiosulfate:glutathione sulfurtransferase [Source:NCBI gene;Acc:101163444])

HSP 1 Score: 91.2781 bits (225), Expect = 1.682e-22
Identity = 39/95 (41.05%), Postives = 64/95 (67.37%), Query Frame = 2 
Query:  197 KAYSVNSAQLHKIVLSEDPVIIDVREENEHRESKINGSINIPLSQLKAAFELPAEDFRKRFGVEKPDKTDDNLILHCRKGIRSFDGLKILHELGF 481
            +A+ V  +QL  ++ + D  + DVR+ +EH+E +I  ++N+PL  L+ + +LP++ F +RF V+ P+K D N++ HCR G RSF  L I H+LGF
Sbjct:   62 QAFVVTHSQLKTMLTNRDIQLFDVRDPDEHQEGRIPQAVNLPLGVLEESLKLPSDHFEQRFKVKAPEKEDANIVFHCRTGKRSFQALGIAHQLGF 156
[Close]
BLAST of Rhodanese-like protein vs. Ensembl Medaka
Match: ENSORLT00000037448.1 (thiosulfate:glutathione sulfurtransferase [Source:NCBI gene;Acc:101160420])

HSP 1 Score: 85.1149 bits (209), Expect = 4.300e-21
Identity = 39/105 (37.14%), Postives = 66/105 (62.86%), Query Frame = 2 
Query:  230 KIVLSEDP--VIIDVREENEHRESKINGSINIPLSQLKAAFELPAEDFRKRFGVEKPDKTDDNLILHCRKGIRSFDGLKILHELGFTRSRHLDGGINDWFEYKTK 538
            +++L E+   V++DVR + E     I GSINIPL  +++AF+LP EDF+ ++G  KP+K   +L+ HC+ G R     K   +LG++++R+  GG  +W + + K
Sbjct:   10 QVLLKENQNLVLVDVRSKGEVDNGHIPGSINIPLDTVESAFKLPPEDFKSKYGKTKPEKDSQDLVFHCQMGRRGETATKQARQLGYSKARNYAGGYMEWSKKQNK 114
[Close]
BLAST of Rhodanese-like protein vs. Ensembl Medaka
Match: ENSORLT00000029861.1 (thiosulfate:glutathione sulfurtransferase [Source:NCBI gene;Acc:101160420])

HSP 1 Score: 85.1149 bits (209), Expect = 4.300e-21
Identity = 39/105 (37.14%), Postives = 66/105 (62.86%), Query Frame = 2 
Query:  230 KIVLSEDP--VIIDVREENEHRESKINGSINIPLSQLKAAFELPAEDFRKRFGVEKPDKTDDNLILHCRKGIRSFDGLKILHELGFTRSRHLDGGINDWFEYKTK 538
            +++L E+   V++DVR + E     I GSINIPL  +++AF+LP EDF+ ++G  KP+K   +L+ HC+ G R     K   +LG++++R+  GG  +W + + K
Sbjct:   10 QVLLKENQNLVLVDVRSKGEVDNGHIPGSINIPLDTVESAFKLPPEDFKSKYGKTKPEKDSQDLVFHCQMGRRGETATKQARQLGYSKARNYAGGYMEWSKKQNK 114
[Close]
BLAST of Rhodanese-like protein vs. Ensembl Medaka
Match: ENSORLT00000003636.2 (thiosulfate:glutathione sulfurtransferase [Source:NCBI gene;Acc:101160420])

HSP 1 Score: 84.7297 bits (208), Expect = 5.892e-21
Identity = 39/105 (37.14%), Postives = 66/105 (62.86%), Query Frame = 2 
Query:  230 KIVLSEDP--VIIDVREENEHRESKINGSINIPLSQLKAAFELPAEDFRKRFGVEKPDKTDDNLILHCRKGIRSFDGLKILHELGFTRSRHLDGGINDWFEYKTK 538
            +++L E+   V++DVR + E     I GSINIPL  +++AF+LP EDF+ ++G  KP+K   +L+ HC+ G R     K   +LG++++R+  GG  +W + + K
Sbjct:   15 QVLLKENQNLVLVDVRSKGEVDNGHIPGSINIPLDTVESAFKLPPEDFKSKYGKTKPEKDSQDLVFHCQMGRRGETATKQARQLGYSKARNYAGGYMEWSKKQNK 119
[Close]
BLAST of Rhodanese-like protein vs. Planmine SMEST
Match: SMESG000079774.1 (SMESG000079774.1)

HSP 1 Score: 332.413 bits (851), Expect = 7.676e-118
Identity = 161/163 (98.77%), Postives = 161/163 (98.77%), Query Frame = 2 
Query:   56 MKVLRYLYHISKGNFIQSYSKKTILTPRFQYINTQRAFLSILPDEVLKAYSVNSAQLHKIVLSEDPVIIDVREENEHRESKINGSINIPLSQLKAAFELPAEDFRKRFGVEKPDKTDDNLILHCRKGIRSFDGLKILHELGFTRSRHLDGGINDWFEYKTKSK 544
            MKVLRYLYHISKGNFIQSYSKKTILTPRFQYINTQRAFLSILPDEVLK YSVNSAQLHKIVL EDPVIIDVREENEHRESKINGSINIPLSQLKAAFELPAEDFRKRFGVEKPDKTDDNLILHCRKGIRSFDGLKILHELGFTRSRHLDGGINDWFEYKTKSK
Sbjct:    1 MKVLRYLYHISKGNFIQSYSKKTILTPRFQYINTQRAFLSILPDEVLKTYSVNSAQLHKIVLGEDPVIIDVREENEHRESKINGSINIPLSQLKAAFELPAEDFRKRFGVEKPDKTDDNLILHCRKGIRSFDGLKILHELGFTRSRHLDGGINDWFEYKTKSK 163
[Close]
BLAST of Rhodanese-like protein vs. Planmine SMEST
Match: SMESG000079774.1 (SMESG000079774.1)

HSP 1 Score: 282.337 bits (721), Expect = 2.400e-98
Identity = 143/163 (87.73%), Postives = 143/163 (87.73%), Query Frame = 2 
Query:   56 MKVLRYLYHISKGNFIQSYSKKTILTPRFQYINTQRAFLSILPDEVLKAYSVNSAQLHKIVLSEDPVIIDVREENEHRESKINGSINIPLSQLKAAFELPAEDFRKRFGVEKPDKTDDNLILHCRKGIRSFDGLKILHELGFTRSRHLDGGINDWFEYKTKSK 544
            MKVLRYLYHISKGNFIQSYSKKTILTPRFQYINTQRAFLSILPDEVLK YSVNSAQLHKIVL EDPVIIDVREENEHRESKINGSINIPLSQLKAAFELPAEDFRK                  RKGIRSFDGLKILHELGFTRSRHLDGGINDWFEYKTKSK
Sbjct:    1 MKVLRYLYHISKGNFIQSYSKKTILTPRFQYINTQRAFLSILPDEVLKTYSVNSAQLHKIVLGEDPVIIDVREENEHRESKINGSINIPLSQLKAAFELPAEDFRK------------------RKGIRSFDGLKILHELGFTRSRHLDGGINDWFEYKTKSK 145
[Close]
BLAST of Rhodanese-like protein vs. Planmine SMEST
Match: SMESG000079781.1 (SMESG000079781.1)

HSP 1 Score: 127.872 bits (320), Expect = 3.068e-37
Identity = 58/118 (49.15%), Postives = 87/118 (73.73%), Query Frame = 2 
Query:  179 LPDEVLKAYSVNSAQLHKIVLSEDPVIIDVREENEHRESKINGSINIPLSQLKAAFELPAEDFRKRFGVEKPDKTDDNLILHCRKGIRSFDGLKILHELGFTRSRHLDGGINDWFEYK 532
            +  E+L+  ++NS+ LH I+L+E+P++IDVRE +E  +  I G++NIPLS++K +F L   +F  +F + KP K + N+IL+CR G RS D L+ILH+LG+T SRHL+GGI DW +YK
Sbjct:   41 ISTEILQMNTINSSTLHHILLNENPILIDVREISEFNQGNIEGALNIPLSRVKKSFNLSEINFLNKFSLTKPVK-NSNIILYCRSGRRSLDALRILHKLGYTDSRHLEGGILDWIDYK 157
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BLAST of Rhodanese-like protein vs. Planmine SMEST
Match: SMESG000079781.1 (SMESG000079781.1)

HSP 1 Score: 127.872 bits (320), Expect = 3.180e-37
Identity = 58/118 (49.15%), Postives = 87/118 (73.73%), Query Frame = 2 
Query:  179 LPDEVLKAYSVNSAQLHKIVLSEDPVIIDVREENEHRESKINGSINIPLSQLKAAFELPAEDFRKRFGVEKPDKTDDNLILHCRKGIRSFDGLKILHELGFTRSRHLDGGINDWFEYK 532
            +  E+L+  ++NS+ LH I+L+E+P++IDVRE +E  +  I G++NIPLS++K +F L   +F  +F + KP K + N+IL+CR G RS D L+ILH+LG+T SRHL+GGI DW +YK
Sbjct:   40 ISTEILQMNTINSSTLHHILLNENPILIDVREISEFNQGNIEGALNIPLSRVKKSFNLSEINFLNKFSLTKPVK-NSNIILYCRSGRRSLDALRILHKLGYTDSRHLEGGILDWIDYK 156
[Close]
BLAST of Rhodanese-like protein vs. Planmine SMEST
Match: SMESG000030828.1 (SMESG000030828.1)

HSP 1 Score: 109.768 bits (273), Expect = 1.223e-30
Identity = 50/112 (44.64%), Postives = 74/112 (66.07%), Query Frame = 2 
Query:  185 DEVLKAYSVNSAQLHKIVLSEDPVIIDVREENEHRESKINGSINIPLSQLKAAFELPAEDFRKRFGVEKPDKTDDNLILHCRKGIRSFDGLKILHELGFTRSRHLDGGINDW 520
            D+V   ++++ A L   + + +  +IDVR   E +E KI G+INIP+ ++K AFEL  E+F K+FG+ KP+KT  N+IL+C  G RS   L++LHE+G+T  RHL GG   W
Sbjct:   12 DDVATTHAISVADLKAAIKTSELQVIDVRSPEEVKEGKIEGAINIPIGEIKEAFELSNEEFEKKFGMPKPEKTSRNIILNCLGGGRSGKALRLLHEMGYTNFRHLAGGYGAW 123
The following BLAST results are available for this feature:
BLAST of Rhodanese-like protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 4
Match NameE-valueIdentityDescription
TSTD13.857e-1629.36thiosulfate sulfurtransferase like domain containi... [more]
TSTD15.213e-1629.36thiosulfate sulfurtransferase like domain containi... [more]
TSTD17.522e-1229.47thiosulfate sulfurtransferase like domain containi... [more]
TSTD13.798e-827.94thiosulfate sulfurtransferase like domain containi... [more]
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BLAST of Rhodanese-like protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rhodanese-like protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CG122791.253e-1239.33gene:FBgn0038080 transcript:FBtr0082656[more]
CG60003.598e-1130.95gene:FBgn0039145 transcript:FBtr0334920[more]
CG60003.598e-1130.95gene:FBgn0039145 transcript:FBtr0343101[more]
CG44562.713e-1034.04gene:FBgn0001228 transcript:FBtr0100655[more]
CG44562.713e-1034.04gene:FBgn0001228 transcript:FBtr0303474[more]
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BLAST of Rhodanese-like protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
si:ch211-161h7.84.336e-2540.57si:ch211-161h7.8 [Source:ZFIN;Acc:ZDB-GENE-091204-... [more]
CABZ01058650.11.488e-2138.32thiosulfate sulfurtransferase/rhodanese-like domai... [more]
si:dkey-165n16.16.051e-2139.25si:dkey-165n16.1 [Source:ZFIN;Acc:ZDB-GENE-100922-... [more]
BX537102.23.329e-1230.00si:dkey-34l15.1 [Source:NCBI gene;Acc:563265][more]
TSTD11.818e-1129.09si:dkey-34l15.1 [Source:ZFIN;Acc:ZDB-GENE-081107-6... [more]
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BLAST of Rhodanese-like protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ENSXETT00000042802.12.563e-1736.36thiosulfate sulfurtransferase (rhodanese)-like dom... [more]
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BLAST of Rhodanese-like protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Tstd13.873e-1631.78thiosulfate sulfurtransferase (rhodanese)-like dom... [more]
Tstd31.994e-1535.78thiosulfate sulfurtransferase (rhodanese)-like dom... [more]
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BLAST of Rhodanese-like protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q8NFU3|TSTD1_HUMAN1.852e-1529.36Thiosulfate:glutathione sulfurtransferase OS=Homo ... [more]
sp|Q9D0B5|TSTD3_MOUSE1.395e-1435.78Thiosulfate sulfurtransferase/rhodanese-like domai... [more]
sp|Q10215|RDL_SCHPO3.219e-1335.05Putative thiosulfate sulfurtransferase, mitochondr... [more]
sp|Q12305|RDL1_YEAST1.200e-1233.65Thiosulfate:glutathione sulfurtransferase OS=Sacch... [more]
sp|Q08742|RDL2_YEAST1.925e-1028.69Thiosulfate sulfurtransferase RDL2, mitochondrial ... [more]
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BLAST of Rhodanese-like protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A3P9HXG01.258e-2540.35Si:ch211-161h7.8 OS=Oryzias latipes OX=8090 PE=4 S... [more]
A0A437C7V25.772e-2540.35Rhodanese domain-containing protein OS=Oryzias jav... [more]
H2LZK56.246e-2539.47Si:ch211-161h7.8 OS=Oryzias latipes OX=8090 GN=LOC... [more]
A0A3B3BJ881.209e-2440.91Si:ch211-161h7.8 OS=Oryzias melastigma OX=30732 PE... [more]
A0A3B3BK682.355e-2440.91Si:ch211-161h7.8 OS=Oryzias melastigma OX=30732 PE... [more]
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BLAST of Rhodanese-like protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 4
Match NameE-valueIdentityDescription
si:ch211-161h7.87.963e-2639.50thiosulfate sulfurtransferase/rhodanese-like domai... [more]
ENSAMXT00000037356.15.419e-1935.24thiosulfate sulfurtransferase/rhodanese-like domai... [more]
si:dkey-34l15.19.804e-1435.79thiosulfate sulfurtransferase/rhodanese-like domai... [more]
si:dkey-34l15.11.644e-1037.18thiosulfate sulfurtransferase/rhodanese-like domai... [more]
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BLAST of Rhodanese-like protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 2
Match NameE-valueIdentityDescription
ENSPMAT00000006076.18.970e-1935.71pep scaffold:Pmarinus_7.0:GL476493:725894:734457:1... [more]
si:ch211-161h7.81.201e-1840.45si:ch211-161h7.8 [Source:ZFIN;Acc:ZDB-GENE-091204-... [more]
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BLAST of Rhodanese-like protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 2
Match NameE-valueIdentityDescription
RDL11.728e-1433.65Thiosulfate sulfurtransferase; contains a rhodanes... [more]
RDL22.772e-1228.69Protein with rhodanese activity; contains a rhodan... [more]
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BLAST of Rhodanese-like protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO340762.461e-2132.86Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO263971.215e-2040.45Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO298854.589e-2037.74Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO340754.180e-1935.96Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO340741.550e-1638.64Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Rhodanese-like protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
si:ch211-161h7.82.351e-2839.47thiosulfate:glutathione sulfurtransferase [Source:... [more]
si:ch211-161h7.81.682e-2241.05thiosulfate:glutathione sulfurtransferase [Source:... [more]
ENSORLT00000037448.14.300e-2137.14thiosulfate:glutathione sulfurtransferase [Source:... [more]
ENSORLT00000029861.14.300e-2137.14thiosulfate:glutathione sulfurtransferase [Source:... [more]
ENSORLT00000003636.25.892e-2137.14thiosulfate:glutathione sulfurtransferase [Source:... [more]
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BLAST of Rhodanese-like protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000079774.17.676e-11898.77SMESG000079774.1[more]
SMESG000079774.12.400e-9887.73SMESG000079774.1[more]
SMESG000079781.13.068e-3749.15SMESG000079781.1[more]
SMESG000079781.13.180e-3749.15SMESG000079781.1[more]
SMESG000030828.11.223e-3044.64SMESG000030828.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30029080 ID=SMED30029080|Name=Rhodanese-like protein|organism=Schmidtea mediterranea sexual|type=transcript|length=646bp
AATTGTTTAAATAACATAAGTACTTATTGAATTTTGTATTATATTATATT
GCGATATGAAAGTTTTACGATATTTATATCACATTAGTAAAGGAAATTTT
ATTCAATCTTATAGTAAAAAGACAATTTTAACACCCAGGTTTCAATACAT
AAATACTCAACGAGCTTTTTTATCAATTTTACCAGACGAAGTTTTAAAAG
CATATTCTGTAAACAGTGCTCAATTACATAAGATTGTCTTAAGCGAAGAT
CCAGTTATCATTGATGTCCGAGAAGAAAATGAACATCGCGAAAGCAAAAT
TAATGGATCTATTAATATTCCATTATCGCAGTTGAAAGCAGCTTTCGAGC
TTCCTGCGGAGGATTTTCGAAAGAGGTTTGGAGTTGAGAAACCCGATAAA
ACTGATGACAACCTTATATTACATTGCAGAAAAGGGATAAGAAGTTTTGA
TGGGCTAAAAATTTTGCACGAATTAGGATTCACTCGATCAAGACATCTTG
ACGGCGGAATCAACGATTGGTTTGAGTATAAAACTAAATCTAAATGAGAT
TCGAATTTGAATTTTAGTTTTTCCTATTTTATTATTCACATTACCCATTG
ATAAACCTTTGATTTTTATTTAATAAATTCTTTTAGCATTTAAAAA
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protein sequence of SMED30029080-orf-1

>SMED30029080-orf-1 ID=SMED30029080-orf-1|Name=SMED30029080-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=164bp
MKVLRYLYHISKGNFIQSYSKKTILTPRFQYINTQRAFLSILPDEVLKAY
SVNSAQLHKIVLSEDPVIIDVREENEHRESKINGSINIPLSQLKAAFELP
AEDFRKRFGVEKPDKTDDNLILHCRKGIRSFDGLKILHELGFTRSRHLDG
GINDWFEYKTKSK*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
PLANA:0002089reproductive organ
Vocabulary: INTERPRO
TermDefinition
IPR001763Rhodanese-like_dom
Vocabulary: molecular function
TermDefinition
GO:0016740transferase activity