SMED30028985

Overview
NameSMED30028985
Smed IDSMED30028985
Length (bp)1693
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30028985 (SMED30028985) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30028985 (SMED30028985) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30028985 (SMED30028985) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30028985 (SMED30028985) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30028985

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 13

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30028985h1SMcG0012243 Contig5023uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30028985h1SMcG0012243 Contig5023newmark_estsPMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30028985h1SMcG0021295 Contig5023uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30028985h1SMcG0021295 Contig5023newmark_estsPMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
Smed sexual biotypeSMED30028985h1SMcG0021295 Contig55667uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30028985h1SMcG0021295 Contig55667newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
pharynxSMED30028985h1SMcG0021295 DN308448smed_ncbi_20240424PMID:24173799
Cowles et al., 2013		whole organism asexual adult colorimetric in situ hybridization evidence
gutSMED30028985h1SMcG0021295 DN308448smed_ncbi_20240424PMID:24173799
Cowles et al., 2013		whole organism asexual adult colorimetric in situ hybridization evidence
epidermisSMED30028985h1SMcG0021295 DN308448smed_ncbi_20240424PMID:24173799
Cowles et al., 2013		whole organism asexual adult colorimetric in situ hybridization evidence
central nervous systemSMED30028985h1SMcG0021295 DN308448smed_ncbi_20240424PMID:24173799
Cowles et al., 2013		whole organism asexual adult colorimetric in situ hybridization evidence
progenitor cellSMED30028985h1SMcG0021295 DN308448smed_ncbi_20240424PMID:24173799
Cowles et al., 2013		whole organism asexual adult colorimetric in situ hybridization evidence
stem cellSMED30028985h1SMcG0021295 DN308448smed_ncbi_20240424PMID:24173799
Cowles et al., 2013		whole organism asexual adult colorimetric in situ hybridization evidence
parenchymaSMED30028985h1SMcG0021295 DN308448smed_ncbi_20240424PMID:24173799
Cowles et al., 2013		whole organism asexual adult colorimetric in situ hybridization evidence
Note: Hover over icons to view figure legend
Alignments
SMED30028985 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.000488:97071..98770 -8168
Homology
[Close]
BLAST of SMED30028985 vs. Planmine SMEST
Match: SMESG000062657.1 (SMESG000062657.1)

HSP 1 Score: 199.519 bits (506), Expect = 3.447e-59
Identity = 158/158 (100.00%), Postives = 158/158 (100.00%), Query Frame = -3 
Query:  273 KCREIENQVQTLLRQQQRGGCGHSNGYGNSNFQSYNSNASRQSSYRIMDEDSNDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSVESQQSKFDNTDTDNNIAGNQLKDEDNNGKTSNSSEKLSISNQNETGSNXXXXXXXXXXXXXXXXAKRQKRS 746
            KCREIENQVQTLLRQQQRGGCGHSNGYGNSNFQSYNSNASRQSSYRIMDEDSNDLSGSTNSTSRSIISTTSTSTNRNGTTTGLSSSVESQQSKFDNTDTDNNIAGNQLKDEDNNGKTSNSSEKLSISNQNETGSNASSSIEYDDIIDDVIAAKRQKRS
Sbjct:  148 KCREIENQVQTLLRQQQRGGCGHSNGYGNSNFQSYNSNASRQSSYRIMDEDSNDLSGSTNSTSRSIISTTSTSTNRNGTTTGLSSSVESQQSKFDNTDTDNNIAGNQLKDEDNNGKTSNSSEKLSISNQNETGSNASSSIEYDDIIDDVIAAKRQKRS 305
The following BLAST results are available for this feature:
BLAST of SMED30028985 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028985 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028985 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028985 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028985 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028985 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028985 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028985 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028985 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028985 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028985 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028985 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028985 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028985 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000062657.13.447e-59100.00SMESG000062657.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30028985 ID=SMED30028985|Name=SMED30028985|organism=Schmidtea mediterranea sexual|type=transcript|length=1693bp
TGACACAAAACTAATTAAAAAGTTATCCAATTAAAAAGCTCAACCAATTA
AAAGAAACAATCTCAAAATTGAAAATATTAAACAAGAGTTGCATTTATTT
ACAAAAATCATTATCAATAATAATATCACTATCACACTGATCAATCATTG
TACACTACTAACTGTATAATAAAAAAGAAGAGAGAGAGAGAGAGAGAGAA
TGAAATATCCAATTAATAAACAACTTTGTGCATTTAGATAAATATATTTT
CTGATTGAAAAACATTTTTTCAGGATCTTTTCTGTCTTTTCGCAGCAATG
ACGTCATCGATGATGTCGTCATATTCGATTGAGGAGGACGCGTTGGAACC
GGTTTCGTTTTGATTGGAAATAGAAAGCTTTTCACTGGAGTTGGAAGTTT
TCCCGTTATTATCTTCATCTTTCAATTGATTACCAGCGATATTATTATCG
GTGTCGGTATTGTCAAATTTGCTCTGCTGGCTTTCCACAGACGAGCTCAA
TCCAGTCGTCGTTCCGTTGCGATTGGTGCTGGTGCTCGTTGTGGAAATTA
TCGATCGCGACGTTGAGTTTGTGCTGCCCGATAAATCATTGGAATCCTCA
TCCATAATCCGGTACGATGACTGCCTGGACGCGTTTGAATTATAGCTCTG
AAAGTTACTGTTTCCGTAGCCGTTGCTATGGCCACATCCGCCTCTCTGCT
GCTGCCGTAATAGCGTTTGGACTTGATTTTCGATTTCTCGACACTTAAAT
TTATTATTTTCATTAGTTAATTTAGATTGCTGTCGGAAAAAGAAATTTTT
TCTTCGAGATGATTTCTTTGCAATTGGAGAGAGACAAATTTTTGGCTTTA
TAATTTCGTTTATCCCTTCATTTCGACTCGAGATCAACAACAACAACAAC
AAAAATTTGCGGTAAATATCGAAAGACCTGATTATTGATCTCTACAGTCG
AAAGATGAAAATAAATGAAATTTAAAAAAGAAAATATAAACAAAGGTGGA
CAAAAAAGCTAATTTTCCTATTGTAAAACAAAAACCTTTTAAAAAGAGAG
GAAAATAAAGTGTTAATGGAAATATATATATTTGGGAAACAAAAATCTGG
ATTATGAAGAATAAACAAAAAACGTCCTTATTTCAGCCGATGAAAAAGAT
GCCGTATAGAAAATAGAAAGCCAATAAAATCCTATAAGCAATGTACTTTT
CAATATCTTCTATAACTTGAGAGAGGAAAATAGACTTTCTCGTTACTCGG
AAAAGATAATCTAGGTAAATTTAACTCAGAATGCGAAGCGTGATTAAATT
TTTTTTATAAATAAAGATTATATTTCTCAAAATCTTACGGAGAAATACTT
TTTATGAAGAAAAACCCGAACTGTAAGAAAAAGTTGATGAAAATATAAAG
ACAGATTGGAAATTAAAGCTTGATGAAGAATGTCATACCAAAATATCCTG
AAAGGAAGGAATATGACTATAAGTCATGCAATACAACCGTGACATTTATA
ACCATAACCAAGATCCGAGTCTGAAGTAATTTTTTGAAGTAAAATGTTCT
TTGAAAAGAGTATATTTCGACAGTTTTCCTGCAATCATCAGGTAGAATAG
TTCGAACTGTGTATATTGTAGAATAGATAAACAAGTTTTAAAAGTTTTAT
TAATTTTAAAATAAATATATTTTTTAATTTTTGATAATTTTGT
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protein sequence of SMED30028985-orf-1

>SMED30028985-orf-1 ID=SMED30028985-orf-1|Name=SMED30028985-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=112bp
MDEDSNDLSGSTNSTSRSIISTTSTSTNRNGTTTGLSSSVESQQSKFDNT
DTDNNIAGNQLKDEDNNGKTSNSSEKLSISNQNETGSNASSSIEYDDIID
DVIAAKRQKRS*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000026gut
PLANA:0000034epidermis
PLANA:0000070intestinal phagocyte
PLANA:0000103central nervous system
PLANA:0000120progenitor cell
PLANA:0000121stem cell
PLANA:0002142parenchyma
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableTMHMMTMhelixcoord: 21..40