SMED30028923

Overview
NameSMED30028923
Smed IDSMED30028923
Length (bp)703
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30028923 (SMED30028923) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30028923 (SMED30028923) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30028923 (SMED30028923) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30028923 (SMED30028923) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30028923

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 8

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
prepharyngeal regionSMED30028923h1SMcG0017944 SmedASXL_010817SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30028923h1SMcG0017944 SmedASXL_010817SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
nervous systemSMED30028923h1SMcG0017944 dd_Smed_v4_10389_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30028923h1SMcG0017944 dd_Smed_v4_10389_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30028923h1SMcG0017944 dd_Smed_v4_10389_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30028923h1SMcG0017944 dd_Smed_v4_10389_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30028923h1SMcG0017944 OX_Smed_1.0.17722ox_Smed_v2PMID:24238224
Kao et al., 2013		whole organism asexual adult RNA-sequencing evidence
nervous systemSMED30028923h1SMcG0017944 dd_Smed_v4_10389_0_1dd_Smed_v4PMID:30962434
Cote et al., 2019		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30028923 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.001554:65548..66296 -3390
Homology
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BLAST of SMED30028923 vs. Planmine SMEST
Match: SMESG000020133.1 (SMESG000020133.1)

HSP 1 Score: 247.284 bits (630), Expect = 1.472e-84
Identity = 120/121 (99.17%), Postives = 121/121 (100.00%), Query Frame = 1 
Query:   70 MKLIYCVLWICCFISTNGFWCPPCFQQECPKNDLNCRLENTVSDRCNCCNVCGITEGEICTSISSSRCANGLRCVTNFGCRYQYYLPTEIMVRGRCEKEKPKEPNTPDHCYKIEFLMKQVP 432
            MKLIYCVLWICCFISTNGFWCPPCFQQECPKNDLNCRLENTVSDRCNCCNVCGITEGEICTSISSSRCANGLRCVTNFGCRYQYYLPTEIMVRGRCEKEKPKEPNTPDHCYKIEF+MKQVP
Sbjct:    1 MKLIYCVLWICCFISTNGFWCPPCFQQECPKNDLNCRLENTVSDRCNCCNVCGITEGEICTSISSSRCANGLRCVTNFGCRYQYYLPTEIMVRGRCEKEKPKEPNTPDHCYKIEFIMKQVP 121
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BLAST of SMED30028923 vs. Planmine SMEST
Match: SMESG000020144.1 (SMESG000020144.1)

HSP 1 Score: 115.161 bits (287), Expect = 1.597e-32
Identity = 55/103 (53.40%), Postives = 66/103 (64.08%), Query Frame = 1 
Query:  118 NGFWCPPCFQQECPKNDLNCRLENTVSDRCNCCNVCGITEGEICTSISSSRCANGLRCVTNFGCRYQYYLPTEIMVRGRCEKEKPKEPNTPDHCYKIEFLMKQ 426
              FWCPPC    C     +C     V   C+CC VCG+ EGE CTS S +RCANGLRC T+FGCRYQYYLP +  V G C +EKP E  TP +CY + ++MKQ
Sbjct:   17 QAFWCPPCDMSSCTPVSSSCPESRIVPSICSCCKVCGLQEGEKCTSNSGTRCANGLRCETSFGCRYQYYLPWQTPVSGTCVQEKPDEDETPSYCYDMGWIMKQ 119
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BLAST of SMED30028923 vs. Planmine SMEST
Match: SMESG000020144.1 (SMESG000020144.1)

HSP 1 Score: 100.138 bits (248), Expect = 4.655e-27
Identity = 48/85 (56.47%), Postives = 59/85 (69.41%), Query Frame = 1 
Query:  172 NCRLENTVSDRCNCCNVCGITEGEICTSISSSRCANGLRCVTNFGCRYQYYLPTEIMVRGRCEKEKPKEPNTPDHCYKIEFLMKQ 426
            +C     V   C+CC VCG+ EGE CTS S +RCANGLRC T+FGCRYQYYLP +  V G C +EKP E  TP +CY + ++MKQ
Sbjct:   10 SCPESRIVPSICSCCKVCGLQEGEKCTSNSGTRCANGLRCETSFGCRYQYYLPWQTPVSGTCVQEKPDEDETPSYCYDMGWIMKQ 94
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BLAST of SMED30028923 vs. Planmine SMEST
Match: SMESG000039126.1 (SMESG000039126.1)

HSP 1 Score: 84.7297 bits (208), Expect = 7.985e-21
Identity = 49/119 (41.18%), Postives = 66/119 (55.46%), Query Frame = 1 
Query:   70 MKLIYCVLWICCFISTNGFWCPPCFQQECPKNDLNCRLENTVSDRCNCCNVCGITEGEICTSISSSRCANGLRCVTNFGCRYQYYLPTEIMVRGRCEKEKPKEPNTPDHCYKIEFLMKQ 426
            MK+ + +L +  +  T    C  CF   C    LNC     + D CNCC VC   EG+IC S +   CA GLRCVT +GCRY  +LP  + ++G CEKE P EP    +CY+  +L +Q
Sbjct:    1 MKVAFILLLLIGY--TYSLSCK-CFASSCNSVKLNCPRSQQIRDSCNCCYVCANLEGQICES-NIKACAPGLRCVTEYGCRYSNHLPWFLPIQGVCEKELPNEPPADINCYRYSYLQQQ 115
[Close]
BLAST of SMED30028923 vs. Planmine SMEST
Match: SMESG000020136.1 (SMESG000020136.1)

HSP 1 Score: 76.6406 bits (187), Expect = 8.869e-18
Identity = 45/118 (38.14%), Postives = 64/118 (54.24%), Query Frame = 1 
Query:   76 LIYCVLWICCFISTNGFWCPPCFQQECPKNDLNCRLENTVSDRCNCCNVCGITEGEICTSISSSRCANGLRCVTNFGCRYQYYLPTEIMVRGRCEKEKPKEPNTPDHCYKIEFLMKQV 429
            +++ ++  C  +S     C PC + +C   +LNC     V   C CCNVCG+ EG+ICTS S   C  G  C T FGCRY+  LP  I  +G C KEK  E     +CY  ++++ Q+
Sbjct:    6 IVFSIIGYCSSLS-----CIPCNEVKCNDANLNCPKSQFVKGICGCCNVCGLQEGKICTSFSI--CMKGTLCETAFGCRYRNTLPFYIPFKGVCVKEKENEEPIEPNCYNYKYVVSQI 116
The following BLAST results are available for this feature:
BLAST of SMED30028923 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028923 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028923 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028923 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028923 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028923 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028923 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028923 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028923 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028923 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028923 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028923 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028923 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028923 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000020133.11.472e-8499.17SMESG000020133.1[more]
SMESG000020144.11.597e-3253.40SMESG000020144.1[more]
SMESG000020144.14.655e-2756.47SMESG000020144.1[more]
SMESG000039126.17.985e-2141.18SMESG000039126.1[more]
SMESG000020136.18.869e-1838.14SMESG000020136.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30028923 ID=SMED30028923|Name=SMED30028923|organism=Schmidtea mediterranea sexual|type=transcript|length=703bp
ATTAAAACAAACAATTGGACACACAGACTCGATTAAACAAACAAATTTGT
TTTTACAGAATTCATAAATATGAAACTAATATATTGTGTTCTTTGGATTT
GTTGCTTTATTTCGACAAACGGTTTCTGGTGCCCGCCCTGTTTCCAACAA
GAATGTCCGAAAAACGATTTAAACTGTCGACTGGAAAACACTGTTTCAGA
TCGGTGCAATTGTTGCAATGTATGCGGAATCACTGAAGGCGAGATCTGTA
CGTCTATTTCTTCATCAAGATGCGCTAACGGTTTGAGGTGCGTCACGAAT
TTTGGATGTCGGTATCAATATTATCTACCCACTGAAATTATGGTCAGAGG
AAGATGTGAAAAAGAAAAACCAAAAGAACCAAATACACCAGACCATTGCT
ACAAAATCGAATTTCTAATGAAACAAGTACCATAAATCCGGACTTTGGTT
TTTAATGGTTAAATTGTTCCTTGAAATAAAAAGTTTTTTACATTTAGTAC
CAAATCTGGACAGTAGAAATCTTACAAAATTTGGTAAGGAGGCTTTCTCT
TGATGATCCAACGGCAATGTCTACAGACAAAACCTTAAAATCTCCAACAT
TAAATTGGATTCCATAAACATTTAACACAAAACGTAAACTTTTCAGTCCA
TTTTCTAACTTTTAAAAAGCTAAGTATGCAGTTTTAAACGTTTATTCTCA
CAT
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protein sequence of SMED30028923-orf-1

>SMED30028923-orf-1 ID=SMED30028923-orf-1|Name=SMED30028923-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=122bp
MKLIYCVLWICCFISTNGFWCPPCFQQECPKNDLNCRLENTVSDRCNCCN
VCGITEGEICTSISSSRCANGLRCVTNFGCRYQYYLPTEIMVRGRCEKEK
PKEPNTPDHCYKIEFLMKQVP*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000025nervous system
PLANA:0000099neuron
PLANA:0000418head
Vocabulary: INTERPRO
TermDefinition
IPR000867IGFBP-like
IPR009030Growth_fac_rcpt_cys_sf
Vocabulary: biological process
TermDefinition
GO:0001558regulation of cell growth
Vocabulary: cellular component
TermDefinition
GO:0005576extracellular region
Vocabulary: molecular function
TermDefinition
GO:0005520insulin-like growth factor binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000867Insulin-like growth factor-binding protein, IGFBPSMARTSM00121IB_2coord: 19..98
e-value: 4.7E-4
score: 24.9
IPR000867Insulin-like growth factor-binding protein, IGFBPPFAMPF00219IGFBPcoord: 21..74
e-value: 3.2E-9
score: 37.3
IPR000867Insulin-like growth factor-binding protein, IGFBPPROSITEPS51323IGFBP_N_2coord: 17..83
score: 12.71
NoneNo IPR availableGENE3DG3DSA:3.30.60.30coord: 20..112
e-value: 5.5E-6
score: 28.9
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..18
score: 0.858
IPR009030Growth factor receptor cysteine-rich domain superfamilySUPERFAMILYSSF57184Growth factor receptor domaincoord: 19..87