U2 snRNP auxiliary factor large subunit

Overview
NameU2 snRNP auxiliary factor large subunit
Smed IDSMED30028920
Length (bp)967
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of U2 snRNP auxiliary factor large subunit (SMED30028920) t-SNE clustered cells

Violin plots show distribution of expression levels for U2 snRNP auxiliary factor large subunit (SMED30028920) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of U2 snRNP auxiliary factor large subunit (SMED30028920) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for U2 snRNP auxiliary factor large subunit (SMED30028920) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30028920

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 1

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X2 cellSMED30028920SMESG000078029.1 SmedASXL_005625SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Human
Match: U2AF2 (U2 small nuclear RNA auxiliary factor 2 [Source:HGNC Symbol;Acc:HGNC:23156])

HSP 1 Score: 245.743 bits (626), Expect = 1.920e-77
Identity = 123/212 (58.02%), Postives = 157/212 (74.06%), Query Frame = 1 
Query:  352 KKDYKYWDVPPTGYEHMSPSEFKAMQAAGQV-----LPTIYATESGIVNTEQIMDQHPSIGSAVSRQARRIYVGNIPLGVTEQGMMDFFNEQMKLKLFTQADGDPIIAVQINTDKNFAFLEFRSVEETSQGLNLDGIMYNGFPLKLRRPRDYVPLPTTMD--TMLPNSLAAGVVQDTPHKLFIGGLPHYLNEDQVKELLLTFGPLSGFNLVK 966
            KK  KYWDVPP G+EH++P ++KAMQAAGQ+     LPT+  T  G+  T   +   P +GS ++RQARR+YVGNIP G+TE+ MMDFFN QM+L   TQA G+P++AVQIN DKNFAFLEFRSV+ET+Q +  DGI++ G  LK+RRP DY PLP   +  ++    + + VV D+ HKLFIGGLP+YLN+DQVKELL +FGPL  FNLVK
Sbjct:   86 KKVRKYWDVPPPGFEHITPMQYKAMQAAGQIPATALLPTM--TPDGLAVTPTPV---PVVGSQMTRQARRLYVGNIPFGITEEAMMDFFNAQMRLGGLTQAPGNPVLAVQINQDKNFAFLEFRSVDETTQAMAFDGIIFQGQSLKIRRPHDYQPLPGMSENPSVYVPGVVSTVVPDSAHKLFIGGLPNYLNDDQVKELLTSFGPLKAFNLVK 292
[Close]
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Human
Match: U2AF2 (U2 small nuclear RNA auxiliary factor 2 [Source:HGNC Symbol;Acc:HGNC:23156])

HSP 1 Score: 245.358 bits (625), Expect = 2.362e-77
Identity = 123/212 (58.02%), Postives = 157/212 (74.06%), Query Frame = 1 
Query:  352 KKDYKYWDVPPTGYEHMSPSEFKAMQAAGQV-----LPTIYATESGIVNTEQIMDQHPSIGSAVSRQARRIYVGNIPLGVTEQGMMDFFNEQMKLKLFTQADGDPIIAVQINTDKNFAFLEFRSVEETSQGLNLDGIMYNGFPLKLRRPRDYVPLPTTMD--TMLPNSLAAGVVQDTPHKLFIGGLPHYLNEDQVKELLLTFGPLSGFNLVK 966
            KK  KYWDVPP G+EH++P ++KAMQAAGQ+     LPT+  T  G+  T   +   P +GS ++RQARR+YVGNIP G+TE+ MMDFFN QM+L   TQA G+P++AVQIN DKNFAFLEFRSV+ET+Q +  DGI++ G  LK+RRP DY PLP   +  ++    + + VV D+ HKLFIGGLP+YLN+DQVKELL +FGPL  FNLVK
Sbjct:   86 KKVRKYWDVPPPGFEHITPMQYKAMQAAGQIPATALLPTM--TPDGLAVTPTPV---PVVGSQMTRQARRLYVGNIPFGITEEAMMDFFNAQMRLGGLTQAPGNPVLAVQINQDKNFAFLEFRSVDETTQAMAFDGIIFQGQSLKIRRPHDYQPLPGMSENPSVYVPGVVSTVVPDSAHKLFIGGLPNYLNDDQVKELLTSFGPLKAFNLVK 292
[Close]
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Human
Match: U2AF2 (U2 small nuclear RNA auxiliary factor 2 [Source:HGNC Symbol;Acc:HGNC:23156])

HSP 1 Score: 162.925 bits (411), Expect = 2.377e-47
Identity = 81/130 (62.31%), Postives = 97/130 (74.62%), Query Frame = 1 
Query:  589 MMDFFNEQMKLKLFTQADGDPIIAVQINTDKNFAFLEFRSVEETSQGLNLDGIMYNGFPLKLRRPRDYVPLPTTMDTMLPNSLAAGVVQ----DTPHKLFIGGLPHYLNEDQVKELLLTFGPLSGFNLVK 966
            MMDFFN QM+L   TQA G+P++AVQIN DKNFAFLEFRSV+ET+Q +  DGI++ G  LK+RRP DY PLP   +   P+    GVV     D+ HKLFIGGLP+YLN+DQVKELL +FGPL  FNLVK
Sbjct:    1 MMDFFNAQMRLGGLTQAPGNPVLAVQINQDKNFAFLEFRSVDETTQAMAFDGIIFQGQSLKIRRPHDYQPLPGMSEN--PSVYVPGVVSTVVPDSAHKLFIGGLPNYLNDDQVKELLTSFGPLKAFNLVK 128
[Close]
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Human
Match: U2AF2 (U2 small nuclear RNA auxiliary factor 2 [Source:HGNC Symbol;Acc:HGNC:23156])

HSP 1 Score: 85.8853 bits (211), Expect = 1.341e-20
Identity = 44/82 (53.66%), Postives = 58/82 (70.73%), Query Frame = 1 
Query:  352 KKDYKYWDVPPTGYEHMSPSEFKAMQAAGQV-----LPTIYATESGIVNTEQIMDQHPSIGSAVSRQARRIYVGNIPLGVTE 582
            KK  KYWDVPP G+EH++P ++KAMQAAGQ+     LPT+  T  G+  T   +   P +GS ++RQARR+YVGNIP G+TE
Sbjct:   10 KKVRKYWDVPPPGFEHITPMQYKAMQAAGQIPATALLPTM--TPDGLAVTPTPV---PVVGSQMTRQARRLYVGNIPFGITE 86
[Close]
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Celegans
Match: uaf-1 (Splicing factor U2AF 65 kDa subunit [Source:UniProtKB/Swiss-Prot;Acc:P90978])

HSP 1 Score: 214.157 bits (544), Expect = 1.163e-65
Identity = 106/204 (51.96%), Postives = 141/204 (69.12%), Query Frame = 1 
Query:  355 KDYKYWDVPPTGYEHMSPSEFKAMQAAGQVLPTIYATESGIVNTEQIMDQHPSIGSAVSRQARRIYVGNIPLGVTEQGMMDFFNEQMKLKLFTQADGDPIIAVQINTDKNFAFLEFRSVEETSQGLNLDGIMYNGFPLKLRRPRDYVPLPTT--MDTMLPNSLAAGVVQDTPHKLFIGGLPHYLNEDQVKELLLTFGPLSGFNL 960
            K Y++WDVPPTG+E  +P E+K MQAAGQV     + +S +          P +G +V+ Q+RR+YVGNIP G  E+ M+DFFN+QM L    QA G+PI+  QIN DKNFAF+EFRS++ET+ G+  DGI + G  LK+RRPRDY P   T  M++ +P S    +V D+ +K+FIGGLP+YL EDQVKELL +FGPL  F+L
Sbjct:  109 KKYRFWDVPPTGFETTTPMEYKNMQAAGQV--PRGSVQSAV----------PVVGPSVTCQSRRLYVGNIPFGCNEEAMLDFFNQQMHLCGLAQAPGNPILLCQINLDKNFAFIEFRSIDETTAGMAFDGINFMGQQLKVRRPRDYQPSQNTFDMNSRMPVST---IVVDSANKIFIGGLPNYLTEDQVKELLCSFGPLKAFSL 297
[Close]
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Celegans
Match: uaf-1 (Splicing factor U2AF 65 kDa subunit [Source:UniProtKB/Swiss-Prot;Acc:P90978])

HSP 1 Score: 214.157 bits (544), Expect = 1.418e-65
Identity = 106/204 (51.96%), Postives = 141/204 (69.12%), Query Frame = 1 
Query:  355 KDYKYWDVPPTGYEHMSPSEFKAMQAAGQVLPTIYATESGIVNTEQIMDQHPSIGSAVSRQARRIYVGNIPLGVTEQGMMDFFNEQMKLKLFTQADGDPIIAVQINTDKNFAFLEFRSVEETSQGLNLDGIMYNGFPLKLRRPRDYVPLPTT--MDTMLPNSLAAGVVQDTPHKLFIGGLPHYLNEDQVKELLLTFGPLSGFNL 960
            K Y++WDVPPTG+E  +P E+K MQAAGQV     + +S +          P +G +V+ Q+RR+YVGNIP G  E+ M+DFFN+QM L    QA G+PI+  QIN DKNFAF+EFRS++ET+ G+  DGI + G  LK+RRPRDY P   T  M++ +P S    +V D+ +K+FIGGLP+YL EDQVKELL +FGPL  F+L
Sbjct:  112 KKYRFWDVPPTGFETTTPMEYKNMQAAGQV--PRGSVQSAV----------PVVGPSVTCQSRRLYVGNIPFGCNEEAMLDFFNQQMHLCGLAQAPGNPILLCQINLDKNFAFIEFRSIDETTAGMAFDGINFMGQQLKVRRPRDYQPSQNTFDMNSRMPVST---IVVDSANKIFIGGLPNYLTEDQVKELLCSFGPLKAFSL 300
[Close]
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Celegans
Match: uaf-1 (Splicing factor U2AF 65 kDa subunit [Source:UniProtKB/Swiss-Prot;Acc:P90978])

HSP 1 Score: 214.157 bits (544), Expect = 2.358e-65
Identity = 106/204 (51.96%), Postives = 141/204 (69.12%), Query Frame = 1 
Query:  355 KDYKYWDVPPTGYEHMSPSEFKAMQAAGQVLPTIYATESGIVNTEQIMDQHPSIGSAVSRQARRIYVGNIPLGVTEQGMMDFFNEQMKLKLFTQADGDPIIAVQINTDKNFAFLEFRSVEETSQGLNLDGIMYNGFPLKLRRPRDYVPLPTT--MDTMLPNSLAAGVVQDTPHKLFIGGLPHYLNEDQVKELLLTFGPLSGFNL 960
            K Y++WDVPPTG+E  +P E+K MQAAGQV     + +S +          P +G +V+ Q+RR+YVGNIP G  E+ M+DFFN+QM L    QA G+PI+  QIN DKNFAF+EFRS++ET+ G+  DGI + G  LK+RRPRDY P   T  M++ +P S    +V D+ +K+FIGGLP+YL EDQVKELL +FGPL  F+L
Sbjct:  134 KKYRFWDVPPTGFETTTPMEYKNMQAAGQV--PRGSVQSAV----------PVVGPSVTCQSRRLYVGNIPFGCNEEAMLDFFNQQMHLCGLAQAPGNPILLCQINLDKNFAFIEFRSIDETTAGMAFDGINFMGQQLKVRRPRDYQPSQNTFDMNSRMPVST---IVVDSANKIFIGGLPNYLTEDQVKELLCSFGPLKAFSL 322
[Close]
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Fly
Match: U2af50 (gene:FBgn0005411 transcript:FBtr0307178)

HSP 1 Score: 236.113 bits (601), Expect = 1.298e-74
Identity = 115/211 (54.50%), Postives = 152/211 (72.04%), Query Frame = 1 
Query:  352 KKDYKYWDVPPTGYEHMSPSEFKAMQAAGQVLPTIYATESGIVNTEQIMDQHPSIGSAVSRQARRIYVGNIPLGVTEQGMMDFFNEQMKLKLFTQADGDPIIAVQINTDKNFAFLEFRSVEETSQGLNLDGIMYNGFPLKLRRPRDYVPLPTTMDT--MLPNSLAAGVVQ----DTPHKLFIGGLPHYLNEDQVKELLLTFGPLSGFNLVK 966
            +K   YWDVPP G+EH++P ++KAMQA+GQ+  ++      + +T Q     P +GS ++RQARR+YVGNIP GVTE+ MM+FFN+QM L    QA G P++A QIN DKNFAFLEFRS++ET+Q +  DGI   G  LK+RRP DY P+P   DT  + P  +++GV+     D+PHK+FIGGLP+YLN+DQVKELLL+FG L  FNLVK
Sbjct:   38 RKPSLYWDVPPPGFEHITPMQYKAMQASGQIPASV------VPDTPQTA--VPVVGSTITRQARRLYVGNIPFGVTEEEMMEFFNQQMHLVGLAQAAGSPVLACQINLDKNFAFLEFRSIDETTQAMAFDGINLKGQSLKIRRPHDYQPMPGITDTPAIKPAVVSSGVISTVVPDSPHKIFIGGLPNYLNDDQVKELLLSFGKLRAFNLVK 240
[Close]
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Fly
Match: U2af50 (gene:FBgn0005411 transcript:FBtr0074234)

HSP 1 Score: 236.113 bits (601), Expect = 1.298e-74
Identity = 115/211 (54.50%), Postives = 152/211 (72.04%), Query Frame = 1 
Query:  352 KKDYKYWDVPPTGYEHMSPSEFKAMQAAGQVLPTIYATESGIVNTEQIMDQHPSIGSAVSRQARRIYVGNIPLGVTEQGMMDFFNEQMKLKLFTQADGDPIIAVQINTDKNFAFLEFRSVEETSQGLNLDGIMYNGFPLKLRRPRDYVPLPTTMDT--MLPNSLAAGVVQ----DTPHKLFIGGLPHYLNEDQVKELLLTFGPLSGFNLVK 966
            +K   YWDVPP G+EH++P ++KAMQA+GQ+  ++      + +T Q     P +GS ++RQARR+YVGNIP GVTE+ MM+FFN+QM L    QA G P++A QIN DKNFAFLEFRS++ET+Q +  DGI   G  LK+RRP DY P+P   DT  + P  +++GV+     D+PHK+FIGGLP+YLN+DQVKELLL+FG L  FNLVK
Sbjct:   38 RKPSLYWDVPPPGFEHITPMQYKAMQASGQIPASV------VPDTPQTA--VPVVGSTITRQARRLYVGNIPFGVTEEEMMEFFNQQMHLVGLAQAAGSPVLACQINLDKNFAFLEFRSIDETTQAMAFDGINLKGQSLKIRRPHDYQPMPGITDTPAIKPAVVSSGVISTVVPDSPHKIFIGGLPNYLNDDQVKELLLSFGKLRAFNLVK 240
[Close]
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Fly
Match: U2af50 (gene:FBgn0005411 transcript:FBtr0307176)

HSP 1 Score: 235.728 bits (600), Expect = 2.168e-74
Identity = 115/211 (54.50%), Postives = 152/211 (72.04%), Query Frame = 1 
Query:  352 KKDYKYWDVPPTGYEHMSPSEFKAMQAAGQVLPTIYATESGIVNTEQIMDQHPSIGSAVSRQARRIYVGNIPLGVTEQGMMDFFNEQMKLKLFTQADGDPIIAVQINTDKNFAFLEFRSVEETSQGLNLDGIMYNGFPLKLRRPRDYVPLPTTMDT--MLPNSLAAGVVQ----DTPHKLFIGGLPHYLNEDQVKELLLTFGPLSGFNLVK 966
            +K   YWDVPP G+EH++P ++KAMQA+GQ+  ++      + +T Q     P +GS ++RQARR+YVGNIP GVTE+ MM+FFN+QM L    QA G P++A QIN DKNFAFLEFRS++ET+Q +  DGI   G  LK+RRP DY P+P   DT  + P  +++GV+     D+PHK+FIGGLP+YLN+DQVKELLL+FG L  FNLVK
Sbjct:   49 RKPSLYWDVPPPGFEHITPMQYKAMQASGQIPASV------VPDTPQTA--VPVVGSTITRQARRLYVGNIPFGVTEEEMMEFFNQQMHLVGLAQAAGSPVLACQINLDKNFAFLEFRSIDETTQAMAFDGINLKGQSLKIRRPHDYQPMPGITDTPAIKPAVVSSGVISTVVPDSPHKIFIGGLPNYLNDDQVKELLLSFGKLRAFNLVK 251
[Close]
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Fly
Match: U2af50 (gene:FBgn0005411 transcript:FBtr0307177)

HSP 1 Score: 208.379 bits (529), Expect = 1.511e-64
Identity = 104/191 (54.45%), Postives = 137/191 (71.73%), Query Frame = 1 
Query:  412 EFKAMQAAGQVLPTIYATESGIVNTEQIMDQHPSIGSAVSRQARRIYVGNIPLGVTEQGMMDFFNEQMKLKLFTQADGDPIIAVQINTDKNFAFLEFRSVEETSQGLNLDGIMYNGFPLKLRRPRDYVPLPTTMDT--MLPNSLAAGVVQ----DTPHKLFIGGLPHYLNEDQVKELLLTFGPLSGFNLVK 966
            ++KAMQA+GQ+  ++      + +T Q     P +GS ++RQARR+YVGNIP GVTE+ MM+FFN+QM L    QA G P++A QIN DKNFAFLEFRS++ET+Q +  DGI   G  LK+RRP DY P+P   DT  + P  +++GV+     D+PHK+FIGGLP+YLN+DQVKELLL+FG L  FNLVK
Sbjct:    2 QYKAMQASGQIPASV------VPDTPQTA--VPVVGSTITRQARRLYVGNIPFGVTEEEMMEFFNQQMHLVGLAQAAGSPVLACQINLDKNFAFLEFRSIDETTQAMAFDGINLKGQSLKIRRPHDYQPMPGITDTPAIKPAVVSSGVISTVVPDSPHKIFIGGLPNYLNDDQVKELLLSFGKLRAFNLVK 184
[Close]
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Fly
Match: LS2 (gene:FBgn0034834 transcript:FBtr0072052)

HSP 1 Score: 182.57 bits (462), Expect = 9.909e-54
Identity = 95/221 (42.99%), Postives = 136/221 (61.54%), Query Frame = 1 
Query:  370 WDVPPTGYEHMSPSEFKAMQAAGQVLPTIYATESGIVNTEQIMDQHPSIGSAVSRQARRIYVGNIPLGVTEQGMMDFFNEQ-MKLKLFTQA----DGDPIIAVQINTDKNFAFLEFRSVEETSQGLNLDGIMYNGFPLKLRRPRDYVPLPTTMDTMLPNSLA------------------AGVVQDTPHKLFIGGLPHYLNEDQVKELLLTFGPLSGFNLV 963
            WD+PP GY H++P ++KAM A+GQ+   I +   G+   E       +  S ++RQARR+YVGNIP GVT++ MM FFN Q M L    ++    DG+ ++  Q N +KNFAFLEFRS++E SQ LN DG+++ G  LK+RRP DY P+P+   + + +  +                    +V D+P+K+++GGLP  LN+DQVKELL +FG L G NLV
Sbjct:   62 WDIPPEGYNHLTPQQYKAMLASGQIASRILS--DGVHGGES------AAISMITRQARRLYVGNIPFGVTDEEMMQFFNHQIMALGFEAKSSHYMDGNAVLTCQTNLEKNFAFLEFRSIDEASQALNFDGMVFRGQTLKIRRPHDYQPVPSISVSAMESYRSFRVPAINVAQQPAVTLPVTTIVPDSPNKIYVGGLPTCLNQDQVKELLQSFGELKGLNLV 274
[Close]
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Zebrafish
Match: u2af2a (U2 small nuclear RNA auxiliary factor 2a [Source:ZFIN;Acc:ZDB-GENE-050706-131])

HSP 1 Score: 244.588 bits (623), Expect = 2.818e-77
Identity = 124/212 (58.49%), Postives = 155/212 (73.11%), Query Frame = 1 
Query:  367 YWDVPPTGYEHMSPSEFKAMQAAGQV-----LPTIYATESGIVNTEQIMDQHPSIGSAVSRQARRIYVGNIPLGVTEQGMMDFFNEQMKLKLFTQADGDPIIAVQINTDKNFAFLEFRSVEETSQGLNLDGIMYNGFPLKLRRPRDYVPLPTTMD---TMLPNSLAAGVVQ----DTPHKLFIGGLPHYLNEDQVKELLLTFGPLSGFNLVK 966
            YWDVPP G+EH++P ++KAMQAAGQ+     LPT+  T  G+  T   +   P +GS ++RQARR+YVGNIP G+TE+ MMDFFN QM+L   TQA G+P++AVQIN DKNFAFLEFRSV+ET+Q +  DGI++ G  LK+RRP DY PLP   +     +P SL  GVV     D+ HKLFIGGLP+YLN+DQVKELL +FGPL  FNLVK
Sbjct:   84 YWDVPPPGFEHITPMQYKAMQAAGQIPATALLPTM--TPEGLAVTPTPV---PVVGSQMTRQARRLYVGNIPFGITEESMMDFFNAQMRLGGLTQAPGNPVLAVQINQDKNFAFLEFRSVDETTQAMAFDGIIFQGQSLKIRRPHDYQPLPGMSENPSVYVPGSLPVGVVSTVVPDSAHKLFIGGLPNYLNDDQVKELLTSFGPLKAFNLVK 290
[Close]
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Zebrafish
Match: u2af2a (U2 small nuclear RNA auxiliary factor 2a [Source:ZFIN;Acc:ZDB-GENE-050706-131])

HSP 1 Score: 241.121 bits (614), Expect = 6.059e-76
Identity = 120/207 (57.97%), Postives = 154/207 (74.40%), Query Frame = 1 
Query:  367 YWDVPPTGYEHMSPSEFKAMQAAGQV-----LPTIYATESGIVNTEQIMDQHPSIGSAVSRQARRIYVGNIPLGVTEQGMMDFFNEQMKLKLFTQADGDPIIAVQINTDKNFAFLEFRSVEETSQGLNLDGIMYNGFPLKLRRPRDYVPLPTTMD--TMLPNSLAAGVVQDTPHKLFIGGLPHYLNEDQVKELLLTFGPLSGFNLVK 966
            YWDVPP G+EH++P ++KAMQAAGQ+     LPT+  T  G+  T   +   P +GS ++RQARR+YVGNIP G+TE+ MMDFFN QM+L   TQA G+P++AVQIN DKNFAFLEFRSV+ET+Q +  DGI++ G  LK+RRP DY PLP   +  ++    + + VV D+ HKLFIGGLP+YLN+DQVKELL +FGPL  FNLVK
Sbjct:   84 YWDVPPPGFEHITPMQYKAMQAAGQIPATALLPTM--TPEGLAVTPTPV---PVVGSQMTRQARRLYVGNIPFGITEESMMDFFNAQMRLGGLTQAPGNPVLAVQINQDKNFAFLEFRSVDETTQAMAFDGIIFQGQSLKIRRPHDYQPLPGMSENPSVYVPGVVSTVVPDSAHKLFIGGLPNYLNDDQVKELLTSFGPLKAFNLVK 285
[Close]
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Zebrafish
Match: u2af2b (U2 small nuclear RNA auxiliary factor 2b [Source:ZFIN;Acc:ZDB-GENE-040426-1881])

HSP 1 Score: 238.81 bits (608), Expect = 6.342e-75
Identity = 119/207 (57.49%), Postives = 153/207 (73.91%), Query Frame = 1 
Query:  367 YWDVPPTGYEHMSPSEFKAMQAAGQV-----LPTIYATESGIVNTEQIMDQHPSIGSAVSRQARRIYVGNIPLGVTEQGMMDFFNEQMKLKLFTQADGDPIIAVQINTDKNFAFLEFRSVEETSQGLNLDGIMYNGFPLKLRRPRDYVPLPTTMD--TMLPNSLAAGVVQDTPHKLFIGGLPHYLNEDQVKELLLTFGPLSGFNLVK 966
            YWDVPP G+EH++P ++KAMQAAGQ+     LPT+  T  G+  T   +   P +GS ++RQARR+YVGNIP G+TE+ MMDFFN QM+L   TQA G+P++AVQIN DKNFAFLEFRSV+ET+Q +  DGI++    LK+RRP DY PLP   +  ++    + + VV D+ HKLFIGGLP+YLN+DQVKELL +FGPL  FNLVK
Sbjct:   94 YWDVPPPGFEHITPMQYKAMQAAGQIPATALLPTM--TPDGLAVTPTPV---PVVGSQMTRQARRLYVGNIPFGITEESMMDFFNAQMRLGGLTQAPGNPVLAVQINQDKNFAFLEFRSVDETTQAMAFDGIIFQAQSLKIRRPHDYQPLPGMSENPSVYVPGVVSTVVPDSIHKLFIGGLPNYLNDDQVKELLTSFGPLKAFNLVK 295
[Close]
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Zebrafish
Match: u2af2b (U2 small nuclear RNA auxiliary factor 2b [Source:ZFIN;Acc:ZDB-GENE-040426-1881])

HSP 1 Score: 236.884 bits (603), Expect = 4.692e-74
Identity = 120/212 (56.60%), Postives = 153/212 (72.17%), Query Frame = 1 
Query:  367 YWDVPPTGYEHMSPSEFKAMQAAGQV-----LPTIYATESGIVNTEQIMDQHPSIGSAVSRQARRIYVGNIPLGVTEQGMMDFFNEQMKLKLFTQADGDPIIAVQINTDKNFAFLEFRSVEETSQGLNLDGIMYNGFPLKLRRPRDYVPLPTTMD---TMLP----NSLAAGVVQDTPHKLFIGGLPHYLNEDQVKELLLTFGPLSGFNLVK 966
            YWDVPP G+EH++P ++KAMQAAGQ+     LPT+  T  G+  T   +   P +GS ++RQARR+YVGNIP G+TE+ MMDFFN QM+L   TQA G+P++AVQIN DKNFAFLEFRSV+ET+Q +  DGI++    LK+RRP DY PLP   +     +P      + + VV D+ HKLFIGGLP+YLN+DQVKELL +FGPL  FNLVK
Sbjct:   94 YWDVPPPGFEHITPMQYKAMQAAGQIPATALLPTM--TPDGLAVTPTPV---PVVGSQMTRQARRLYVGNIPFGITEESMMDFFNAQMRLGGLTQAPGNPVLAVQINQDKNFAFLEFRSVDETTQAMAFDGIIFQAQSLKIRRPHDYQPLPGMSENPSVYVPAVQFQGVVSTVVPDSIHKLFIGGLPNYLNDDQVKELLTSFGPLKAFNLVK 300
[Close]
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Zebrafish
Match: u2af2b (U2 small nuclear RNA auxiliary factor 2b [Source:ZFIN;Acc:ZDB-GENE-040426-1881])

HSP 1 Score: 186.422 bits (472), Expect = 5.236e-57
Identity = 96/181 (53.04%), Postives = 126/181 (69.61%), Query Frame = 1 
Query:  367 YWDVPPTGYEHMSPSEFKAMQAAGQV-----LPTIYATESGIVNTEQIMDQHPSIGSAVSRQARRIYVGNIPLGVTEQGMMDFFNEQMKLKLFTQADGDPIIAVQINTDKNFAFLEFRSVEETSQGLNLDGIMYNGFPLKLRRPRDYVPLPTTMDT---MLP---NSLAAGVVQDTPHKLF 876
            YWDVPP G+EH++P ++KAMQAAGQ+     LPT+  T  G+  T   +   P +GS ++RQARR+YVGNIP G+TE+ MMDFFN QM+L   TQA G+P++AVQIN DKNFAFLEFRSV+ET+Q +  DGI++    LK+RRP DY PLP   +     +P     + + VV D+ HKLF
Sbjct:   94 YWDVPPPGFEHITPMQYKAMQAAGQIPATALLPTM--TPDGLAVTPTPV---PVVGSQMTRQARRLYVGNIPFGITEESMMDFFNAQMRLGGLTQAPGNPVLAVQINQDKNFAFLEFRSVDETTQAMAFDGIIFQAQSLKIRRPHDYQPLPGMSENPSVYVPVQFQGVVSTVVPDSIHKLF 269
[Close]
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Xenopus
Match: mbp (myelin basic protein [Source:Xenbase;Acc:XB-GENE-959616])

HSP 1 Score: 245.743 bits (626), Expect = 4.494e-78
Identity = 123/212 (58.02%), Postives = 157/212 (74.06%), Query Frame = 1 
Query:  352 KKDYKYWDVPPTGYEHMSPSEFKAMQAAGQV-----LPTIYATESGIVNTEQIMDQHPSIGSAVSRQARRIYVGNIPLGVTEQGMMDFFNEQMKLKLFTQADGDPIIAVQINTDKNFAFLEFRSVEETSQGLNLDGIMYNGFPLKLRRPRDYVPLPTTMD--TMLPNSLAAGVVQDTPHKLFIGGLPHYLNEDQVKELLLTFGPLSGFNLVK 966
            KK  KYWDVPP G+EH++P ++KAMQAAGQ+     LPT+  T  G+  T   +   P +GS ++RQARR+YVGNIP G+TE+ MMDFFN QM+L   TQA G+P++AVQIN DKNFAFLEFRSV+ET+Q +  DGI++ G  LK+RRP DY PLP   +  ++    + + VV D+ HKLFIGGLP+YLN+DQVKELL +FGPL  FNLVK
Sbjct:   34 KKIRKYWDVPPPGFEHITPMQYKAMQAAGQIPATALLPTM--TPDGLAVTPTPV---PVVGSQMTRQARRLYVGNIPFGITEEAMMDFFNAQMRLGGLTQAPGNPVLAVQINQDKNFAFLEFRSVDETTQAMAFDGIIFQGQSLKIRRPHDYQPLPGMSENPSVYVPGVVSTVVPDSAHKLFIGGLPNYLNDDQVKELLTSFGPLKAFNLVK 240
[Close]
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Xenopus
Match: mbp (myelin basic protein [Source:Xenbase;Acc:XB-GENE-959616])

HSP 1 Score: 245.358 bits (625), Expect = 1.556e-77
Identity = 123/212 (58.02%), Postives = 157/212 (74.06%), Query Frame = 1 
Query:  352 KKDYKYWDVPPTGYEHMSPSEFKAMQAAGQV-----LPTIYATESGIVNTEQIMDQHPSIGSAVSRQARRIYVGNIPLGVTEQGMMDFFNEQMKLKLFTQADGDPIIAVQINTDKNFAFLEFRSVEETSQGLNLDGIMYNGFPLKLRRPRDYVPLPTTMD--TMLPNSLAAGVVQDTPHKLFIGGLPHYLNEDQVKELLLTFGPLSGFNLVK 966
            KK  KYWDVPP G+EH++P ++KAMQAAGQ+     LPT+  T  G+  T   +   P +GS ++RQARR+YVGNIP G+TE+ MMDFFN QM+L   TQA G+P++AVQIN DKNFAFLEFRSV+ET+Q +  DGI++ G  LK+RRP DY PLP   +  ++    + + VV D+ HKLFIGGLP+YLN+DQVKELL +FGPL  FNLVK
Sbjct:   71 KKIRKYWDVPPPGFEHITPMQYKAMQAAGQIPATALLPTM--TPDGLAVTPTPV---PVVGSQMTRQARRLYVGNIPFGITEEAMMDFFNAQMRLGGLTQAPGNPVLAVQINQDKNFAFLEFRSVDETTQAMAFDGIIFQGQSLKIRRPHDYQPLPGMSENPSVYVPGVVSTVVPDSAHKLFIGGLPNYLNDDQVKELLTSFGPLKAFNLVK 277
[Close]
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Xenopus
Match: mbp (myelin basic protein [Source:Xenbase;Acc:XB-GENE-959616])

HSP 1 Score: 242.662 bits (618), Expect = 2.911e-76
Identity = 125/219 (57.08%), Postives = 157/219 (71.69%), Query Frame = 1 
Query:  352 KKDYKYWDVPPTGYEHMSPSEFKAMQAAGQV-----LPTIYATESGIVNTEQIMDQHPSIGSAVSRQARRIYVGNIPLGVTEQGMMDFFNEQMKLKLFTQADGDPIIAVQINTDKNFAFLEFRSVEETSQGLNLDGIMYNGFPLKLRRPRDYVPLPTTMDT--------MLPN-SLAAGVVQDTPHKLFIGGLPHYLNEDQVKELLLTFGPLSGFNLVK 966
            KK  KYWDVPP G+EH++P ++KAMQAAGQ+     LPT+  T  G+  T   +   P +GS ++RQARR+YVGNIP G+TE+ MMDFFN QM+L   TQA G+P++AVQIN DKNFAFLEFRSV+ET+Q +  DGI++ G  LK+RRP DY PLP   +          LP   + + VV D+ HKLFIGGLP+YLN+DQVKELL +FGPL  FNLVK
Sbjct:   77 KKIRKYWDVPPPGFEHITPMQYKAMQAAGQIPATALLPTM--TPDGLAVTPTPV---PVVGSQMTRQARRLYVGNIPFGITEEAMMDFFNAQMRLGGLTQAPGNPVLAVQINQDKNFAFLEFRSVDETTQAMAFDGIIFQGQSLKIRRPHDYQPLPGMSENPSVYVPGRNLPQLWVVSTVVPDSAHKLFIGGLPNYLNDDQVKELLTSFGPLKAFNLVK 290
[Close]
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Mouse
Match: U2af2 (U2 small nuclear ribonucleoprotein auxiliary factor (U2AF) 2 [Source:MGI Symbol;Acc:MGI:98886])

HSP 1 Score: 245.743 bits (626), Expect = 1.361e-77
Identity = 123/212 (58.02%), Postives = 157/212 (74.06%), Query Frame = 1 
Query:  352 KKDYKYWDVPPTGYEHMSPSEFKAMQAAGQV-----LPTIYATESGIVNTEQIMDQHPSIGSAVSRQARRIYVGNIPLGVTEQGMMDFFNEQMKLKLFTQADGDPIIAVQINTDKNFAFLEFRSVEETSQGLNLDGIMYNGFPLKLRRPRDYVPLPTTMD--TMLPNSLAAGVVQDTPHKLFIGGLPHYLNEDQVKELLLTFGPLSGFNLVK 966
            KK  KYWDVPP G+EH++P ++KAMQAAGQ+     LPT+  T  G+  T   +   P +GS ++RQARR+YVGNIP G+TE+ MMDFFN QM+L   TQA G+P++AVQIN DKNFAFLEFRSV+ET+Q +  DGI++ G  LK+RRP DY PLP   +  ++    + + VV D+ HKLFIGGLP+YLN+DQVKELL +FGPL  FNLVK
Sbjct:   86 KKVRKYWDVPPPGFEHITPMQYKAMQAAGQIPATALLPTM--TPDGLAVTPTPV---PVVGSQMTRQARRLYVGNIPFGITEEAMMDFFNAQMRLGGLTQAPGNPVLAVQINQDKNFAFLEFRSVDETTQAMAFDGIIFQGQSLKIRRPHDYQPLPGMSENPSVYVPGVVSTVVPDSAHKLFIGGLPNYLNDDQVKELLTSFGPLKAFNLVK 292
[Close]
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Mouse
Match: U2af2 (U2 small nuclear ribonucleoprotein auxiliary factor (U2AF) 2 [Source:MGI Symbol;Acc:MGI:98886])

HSP 1 Score: 245.358 bits (625), Expect = 1.622e-77
Identity = 123/212 (58.02%), Postives = 157/212 (74.06%), Query Frame = 1 
Query:  352 KKDYKYWDVPPTGYEHMSPSEFKAMQAAGQV-----LPTIYATESGIVNTEQIMDQHPSIGSAVSRQARRIYVGNIPLGVTEQGMMDFFNEQMKLKLFTQADGDPIIAVQINTDKNFAFLEFRSVEETSQGLNLDGIMYNGFPLKLRRPRDYVPLPTTMD--TMLPNSLAAGVVQDTPHKLFIGGLPHYLNEDQVKELLLTFGPLSGFNLVK 966
            KK  KYWDVPP G+EH++P ++KAMQAAGQ+     LPT+  T  G+  T   +   P +GS ++RQARR+YVGNIP G+TE+ MMDFFN QM+L   TQA G+P++AVQIN DKNFAFLEFRSV+ET+Q +  DGI++ G  LK+RRP DY PLP   +  ++    + + VV D+ HKLFIGGLP+YLN+DQVKELL +FGPL  FNLVK
Sbjct:   86 KKVRKYWDVPPPGFEHITPMQYKAMQAAGQIPATALLPTM--TPDGLAVTPTPV---PVVGSQMTRQARRLYVGNIPFGITEEAMMDFFNAQMRLGGLTQAPGNPVLAVQINQDKNFAFLEFRSVDETTQAMAFDGIIFQGQSLKIRRPHDYQPLPGMSENPSVYVPGVVSTVVPDSAHKLFIGGLPNYLNDDQVKELLTSFGPLKAFNLVK 292
[Close]
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Mouse
Match: U2af2 (U2 small nuclear ribonucleoprotein auxiliary factor (U2AF) 2 [Source:MGI Symbol;Acc:MGI:98886])

HSP 1 Score: 162.925 bits (411), Expect = 1.632e-47
Identity = 81/130 (62.31%), Postives = 97/130 (74.62%), Query Frame = 1 
Query:  589 MMDFFNEQMKLKLFTQADGDPIIAVQINTDKNFAFLEFRSVEETSQGLNLDGIMYNGFPLKLRRPRDYVPLPTTMDTMLPNSLAAGVVQ----DTPHKLFIGGLPHYLNEDQVKELLLTFGPLSGFNLVK 966
            MMDFFN QM+L   TQA G+P++AVQIN DKNFAFLEFRSV+ET+Q +  DGI++ G  LK+RRP DY PLP   +   P+    GVV     D+ HKLFIGGLP+YLN+DQVKELL +FGPL  FNLVK
Sbjct:    1 MMDFFNAQMRLGGLTQAPGNPVLAVQINQDKNFAFLEFRSVDETTQAMAFDGIIFQGQSLKIRRPHDYQPLPGMSEN--PSVYVPGVVSTVVPDSAHKLFIGGLPNYLNDDQVKELLTSFGPLKAFNLVK 128
[Close]
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Mouse
Match: U2af2 (U2 small nuclear ribonucleoprotein auxiliary factor (U2AF) 2 [Source:MGI Symbol;Acc:MGI:98886])

HSP 1 Score: 85.5001 bits (210), Expect = 1.465e-20
Identity = 44/82 (53.66%), Postives = 58/82 (70.73%), Query Frame = 1 
Query:  352 KKDYKYWDVPPTGYEHMSPSEFKAMQAAGQV-----LPTIYATESGIVNTEQIMDQHPSIGSAVSRQARRIYVGNIPLGVTE 582
            KK  KYWDVPP G+EH++P ++KAMQAAGQ+     LPT+  T  G+  T   +   P +GS ++RQARR+YVGNIP G+TE
Sbjct:   19 KKVRKYWDVPPPGFEHITPMQYKAMQAAGQIPATALLPTM--TPDGLAVTPTPV---PVVGSQMTRQARRLYVGNIPFGITE 95
[Close]
BLAST of U2 snRNP auxiliary factor large subunit vs. UniProt/SwissProt
Match: sp|P26368|U2AF2_HUMAN (Splicing factor U2AF 65 kDa subunit OS=Homo sapiens OX=9606 GN=U2AF2 PE=1 SV=4)

HSP 1 Score: 245.743 bits (626), Expect = 9.221e-77
Identity = 123/212 (58.02%), Postives = 157/212 (74.06%), Query Frame = 1 
Query:  352 KKDYKYWDVPPTGYEHMSPSEFKAMQAAGQV-----LPTIYATESGIVNTEQIMDQHPSIGSAVSRQARRIYVGNIPLGVTEQGMMDFFNEQMKLKLFTQADGDPIIAVQINTDKNFAFLEFRSVEETSQGLNLDGIMYNGFPLKLRRPRDYVPLPTTMD--TMLPNSLAAGVVQDTPHKLFIGGLPHYLNEDQVKELLLTFGPLSGFNLVK 966
            KK  KYWDVPP G+EH++P ++KAMQAAGQ+     LPT+  T  G+  T   +   P +GS ++RQARR+YVGNIP G+TE+ MMDFFN QM+L   TQA G+P++AVQIN DKNFAFLEFRSV+ET+Q +  DGI++ G  LK+RRP DY PLP   +  ++    + + VV D+ HKLFIGGLP+YLN+DQVKELL +FGPL  FNLVK
Sbjct:   86 KKVRKYWDVPPPGFEHITPMQYKAMQAAGQIPATALLPTM--TPDGLAVTPTPV---PVVGSQMTRQARRLYVGNIPFGITEEAMMDFFNAQMRLGGLTQAPGNPVLAVQINQDKNFAFLEFRSVDETTQAMAFDGIIFQGQSLKIRRPHDYQPLPGMSENPSVYVPGVVSTVVPDSAHKLFIGGLPNYLNDDQVKELLTSFGPLKAFNLVK 292
[Close]
BLAST of U2 snRNP auxiliary factor large subunit vs. UniProt/SwissProt
Match: sp|P26369|U2AF2_MOUSE (Splicing factor U2AF 65 kDa subunit OS=Mus musculus OX=10090 GN=U2af2 PE=1 SV=3)

HSP 1 Score: 245.743 bits (626), Expect = 9.523e-77
Identity = 123/212 (58.02%), Postives = 157/212 (74.06%), Query Frame = 1 
Query:  352 KKDYKYWDVPPTGYEHMSPSEFKAMQAAGQV-----LPTIYATESGIVNTEQIMDQHPSIGSAVSRQARRIYVGNIPLGVTEQGMMDFFNEQMKLKLFTQADGDPIIAVQINTDKNFAFLEFRSVEETSQGLNLDGIMYNGFPLKLRRPRDYVPLPTTMD--TMLPNSLAAGVVQDTPHKLFIGGLPHYLNEDQVKELLLTFGPLSGFNLVK 966
            KK  KYWDVPP G+EH++P ++KAMQAAGQ+     LPT+  T  G+  T   +   P +GS ++RQARR+YVGNIP G+TE+ MMDFFN QM+L   TQA G+P++AVQIN DKNFAFLEFRSV+ET+Q +  DGI++ G  LK+RRP DY PLP   +  ++    + + VV D+ HKLFIGGLP+YLN+DQVKELL +FGPL  FNLVK
Sbjct:   86 KKVRKYWDVPPPGFEHITPMQYKAMQAAGQIPATALLPTM--TPDGLAVTPTPV---PVVGSQMTRQARRLYVGNIPFGITEEAMMDFFNAQMRLGGLTQAPGNPVLAVQINQDKNFAFLEFRSVDETTQAMAFDGIIFQGQSLKIRRPHDYQPLPGMSENPSVYVPGVVSTVVPDSAHKLFIGGLPNYLNDDQVKELLTSFGPLKAFNLVK 292
[Close]
BLAST of U2 snRNP auxiliary factor large subunit vs. UniProt/SwissProt
Match: sp|Q24562|U2AF2_DROME (Splicing factor U2AF 50 kDa subunit OS=Drosophila melanogaster OX=7227 GN=U2af50 PE=1 SV=1)

HSP 1 Score: 236.113 bits (601), Expect = 1.301e-73
Identity = 115/211 (54.50%), Postives = 152/211 (72.04%), Query Frame = 1 
Query:  352 KKDYKYWDVPPTGYEHMSPSEFKAMQAAGQVLPTIYATESGIVNTEQIMDQHPSIGSAVSRQARRIYVGNIPLGVTEQGMMDFFNEQMKLKLFTQADGDPIIAVQINTDKNFAFLEFRSVEETSQGLNLDGIMYNGFPLKLRRPRDYVPLPTTMDT--MLPNSLAAGVVQ----DTPHKLFIGGLPHYLNEDQVKELLLTFGPLSGFNLVK 966
            +K   YWDVPP G+EH++P ++KAMQA+GQ+  ++      + +T Q     P +GS ++RQARR+YVGNIP GVTE+ MM+FFN+QM L    QA G P++A QIN DKNFAFLEFRS++ET+Q +  DGI   G  LK+RRP DY P+P   DT  + P  +++GV+     D+PHK+FIGGLP+YLN+DQVKELLL+FG L  FNLVK
Sbjct:   38 RKPSLYWDVPPPGFEHITPMQYKAMQASGQIPASV------VPDTPQTA--VPVVGSTITRQARRLYVGNIPFGVTEEEMMEFFNQQMHLVGLAQAAGSPVLACQINLDKNFAFLEFRSIDETTQAMAFDGINLKGQSLKIRRPHDYQPMPGITDTPAIKPAVVSSGVISTVVPDSPHKIFIGGLPNYLNDDQVKELLLSFGKLRAFNLVK 240
[Close]
BLAST of U2 snRNP auxiliary factor large subunit vs. UniProt/SwissProt
Match: sp|P90978|U2AF2_CAEEL (Splicing factor U2AF 65 kDa subunit OS=Caenorhabditis elegans OX=6239 GN=uaf-1 PE=2 SV=2)

HSP 1 Score: 214.157 bits (544), Expect = 3.000e-64
Identity = 106/204 (51.96%), Postives = 141/204 (69.12%), Query Frame = 1 
Query:  355 KDYKYWDVPPTGYEHMSPSEFKAMQAAGQVLPTIYATESGIVNTEQIMDQHPSIGSAVSRQARRIYVGNIPLGVTEQGMMDFFNEQMKLKLFTQADGDPIIAVQINTDKNFAFLEFRSVEETSQGLNLDGIMYNGFPLKLRRPRDYVPLPTT--MDTMLPNSLAAGVVQDTPHKLFIGGLPHYLNEDQVKELLLTFGPLSGFNL 960
            K Y++WDVPPTG+E  +P E+K MQAAGQV     + +S +          P +G +V+ Q+RR+YVGNIP G  E+ M+DFFN+QM L    QA G+PI+  QIN DKNFAF+EFRS++ET+ G+  DGI + G  LK+RRPRDY P   T  M++ +P S    +V D+ +K+FIGGLP+YL EDQVKELL +FGPL  F+L
Sbjct:  134 KKYRFWDVPPTGFETTTPMEYKNMQAAGQV--PRGSVQSAV----------PVVGPSVTCQSRRLYVGNIPFGCNEEAMLDFFNQQMHLCGLAQAPGNPILLCQINLDKNFAFIEFRSIDETTAGMAFDGINFMGQQLKVRRPRDYQPSQNTFDMNSRMPVST---IVVDSANKIFIGGLPNYLTEDQVKELLCSFGPLKAFSL 322
[Close]
BLAST of U2 snRNP auxiliary factor large subunit vs. UniProt/SwissProt
Match: sp|P90727|U2AF2_CAEBR (Splicing factor U2AF 65 kDa subunit OS=Caenorhabditis briggsae OX=6238 GN=uaf-1 PE=3 SV=2)

HSP 1 Score: 213.386 bits (542), Expect = 4.207e-64
Identity = 103/202 (50.99%), Postives = 136/202 (67.33%), Query Frame = 1 
Query:  355 KDYKYWDVPPTGYEHMSPSEFKAMQAAGQVLPTIYATESGIVNTEQIMDQHPSIGSAVSRQARRIYVGNIPLGVTEQGMMDFFNEQMKLKLFTQADGDPIIAVQINTDKNFAFLEFRSVEETSQGLNLDGIMYNGFPLKLRRPRDYVPLPTTMDTMLPNSLAAGVVQDTPHKLFIGGLPHYLNEDQVKELLLTFGPLSGFNL 960
            K Y++WDVPPTG+E+++P E+K MQA            SG V    +    P +G +V+ Q+RR+YVGNIP G  E+ M+DFFN+QM L    QA G+PI+  QIN DKNFAF+EFRS++ET+ G+  DGI + G  LK+RRPRDY P   T D M      + +V D+ +K+FIGGLP+YL EDQVKELL +FGPL  F+L
Sbjct:  125 KKYRFWDVPPTGFENITPMEYKNMQA------------SGAVPRGSVQSAVPVVGPSVTCQSRRLYVGNIPFGCNEEAMLDFFNQQMHLCNLAQAPGNPILLCQINLDKNFAFIEFRSIDETTAGMAFDGINFMGQQLKVRRPRDYQPSQNTFD-MNARMPVSSIVVDSANKIFIGGLPNYLTEDQVKELLCSFGPLKAFSL 313
[Close]
BLAST of U2 snRNP auxiliary factor large subunit vs. TrEMBL
Match: A0A0L8G0Y0 (U2 snRNP auxiliary factor large subunit OS=Octopus bimaculoides OX=37653 GN=OCBIM_22002760mg PE=3 SV=1)

HSP 1 Score: 265.388 bits (677), Expect = 4.584e-84
Identity = 127/208 (61.06%), Postives = 159/208 (76.44%), Query Frame = 1 
Query:  352 KKDYKYWDVPPTGYEHMSPSEFKAMQAAGQVLPTIYATESGIVNTEQIMDQHPSIGSAVSRQARRIYVGNIPLGVTEQGMMDFFNEQMKLKLFTQADGDPIIAVQINTDKNFAFLEFRSVEETSQGLNLDGIMYNGFPLKLRRPRDYVPLPTTMDT---MLPNSLAAGVVQDTPHKLFIGGLPHYLNEDQVKELLLTFGPLSGFNLVK 966
            +K YKYWD+PP+G+EH++P ++KAMQAAGQ+  TI    + +     +    P  GSA+SRQARR+YVGNIP GVTE+ MMDFFN QMK+    QA+G+PIIAVQIN DKNFAFLEFRSV+ET+Q +  DGI + G  LK+RRPRDY PLP   +T    +P  ++  VVQD+PHK+FIGGLP+YLNEDQVKELL++FGPL  FNLVK
Sbjct:    8 RKPYKYWDIPPSGFEHITPLQYKAMQAAGQIPQTITVPNAALTLDSSV----PFAGSAISRQARRLYVGNIPFGVTEEAMMDFFNHQMKVTGLAQAEGNPIIAVQINLDKNFAFLEFRSVDETTQAMAFDGINFQGQSLKIRRPRDYQPLPGMAETPSVAVPGVVST-VVQDSPHKIFIGGLPNYLNEDQVKELLISFGPLKAFNLVK 210
[Close]
BLAST of U2 snRNP auxiliary factor large subunit vs. TrEMBL
Match: A0A0L8G0P8 (U2 snRNP auxiliary factor large subunit OS=Octopus bimaculoides OX=37653 GN=OCBIM_22002760mg PE=3 SV=1)

HSP 1 Score: 265.388 bits (677), Expect = 4.377e-83
Identity = 127/208 (61.06%), Postives = 159/208 (76.44%), Query Frame = 1 
Query:  352 KKDYKYWDVPPTGYEHMSPSEFKAMQAAGQVLPTIYATESGIVNTEQIMDQHPSIGSAVSRQARRIYVGNIPLGVTEQGMMDFFNEQMKLKLFTQADGDPIIAVQINTDKNFAFLEFRSVEETSQGLNLDGIMYNGFPLKLRRPRDYVPLPTTMDT---MLPNSLAAGVVQDTPHKLFIGGLPHYLNEDQVKELLLTFGPLSGFNLVK 966
            +K YKYWD+PP+G+EH++P ++KAMQAAGQ+  TI    + +     +    P  GSA+SRQARR+YVGNIP GVTE+ MMDFFN QMK+    QA+G+PIIAVQIN DKNFAFLEFRSV+ET+Q +  DGI + G  LK+RRPRDY PLP   +T    +P  ++  VVQD+PHK+FIGGLP+YLNEDQVKELL++FGPL  FNLVK
Sbjct:    8 RKPYKYWDIPPSGFEHITPLQYKAMQAAGQIPQTITVPNAALTLDSSV----PFAGSAISRQARRLYVGNIPFGVTEEAMMDFFNHQMKVTGLAQAEGNPIIAVQINLDKNFAFLEFRSVDETTQAMAFDGINFQGQSLKIRRPRDYQPLPGMAETPSVAVPGVVST-VVQDSPHKIFIGGLPNYLNEDQVKELLISFGPLKAFNLVK 210
[Close]
BLAST of U2 snRNP auxiliary factor large subunit vs. TrEMBL
Match: V4A762 (U2 snRNP auxiliary factor large subunit OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_190527 PE=3 SV=1)

HSP 1 Score: 263.077 bits (671), Expect = 2.368e-81
Identity = 130/210 (61.90%), Postives = 156/210 (74.29%), Query Frame = 1 
Query:  352 KKDYKYWDVPPTGYEHMSPSEFKAMQAAGQV---LPTIYATESGIVNTEQIMDQHPSIGSAVSRQARRIYVGNIPLGVTEQGMMDFFNEQMKLKLFTQADGDPIIAVQINTDKNFAFLEFRSVEETSQGLNLDGIMYNGFPLKLRRPRDYVPLPTTMDTMLPN--SLAAGVVQDTPHKLFIGGLPHYLNEDQVKELLLTFGPLSGFNLVK 966
            KK YKYWD+PP G+EHMS  ++KAMQAAGQ+   +P I A +  + NT       P  GS +SRQARR+YVGNIP GVTE+ MMDFFN QMKL    QADG+P+IAVQIN DKNFAFLE+RSV+ET+Q +  DGI + G  LK+RRPRDY PLP   +    N   + + VVQD+PHK+FIGGLP+YLNEDQVKELL +FGPL  FNLVK
Sbjct:   70 KKPYKYWDIPPAGFEHMSVPQYKAMQAAGQIPAAMPIIQAPQVPVTNTT-----IPFAGSTISRQARRLYVGNIPFGVTEEAMMDFFNGQMKLTGLAQADGNPVIAVQINLDKNFAFLEYRSVDETTQAMAFDGINFQGQSLKIRRPRDYQPLPGLSEQPSINVPGVVSTVVQDSPHKIFIGGLPNYLNEDQVKELLTSFGPLKAFNLVK 274
[Close]
BLAST of U2 snRNP auxiliary factor large subunit vs. TrEMBL
Match: A0A0R3U1R1 (U2 snRNP auxiliary factor large subunit OS=Mesocestoides corti OX=53468 GN=MCOS_LOCUS327 PE=3 SV=1)

HSP 1 Score: 264.618 bits (675), Expect = 3.323e-81
Identity = 128/210 (60.95%), Postives = 161/210 (76.67%), Query Frame = 1 
Query:  361 YKYWDVPPTGYEHMSPSEFKAMQAAGQVLPTIYAT-----ESGIVNTEQIMDQH-PSIGSAVSRQARRIYVGNIPLGVTEQGMMDFFNEQMKLKLFTQADGDPIIAVQINTDKNFAFLEFRSVEETSQGLNLDGIMYNGFPLKLRRPRDYVPLPTTMDT--MLPNSLAAGVVQDTPHKLFIGGLPHYLNEDQVKELLLTFGPLSGFNLVK 966
            YKYWDVPP G+EHM+P ++KA+QA+GQ+   +YA       +   N    ++ + P  GSAV RQARR+YVGNIP   TE+ MMDFFN+QMK +   QA+G+PIIAVQIN +KNFAFLEFRSV+ET+QGL LDG+++N   LKLRRPRDY PLP   +T  ++   + + VVQD+PHK+F+GGLP+YLNEDQVKELLLTFGPL GFNLVK
Sbjct:  111 YKYWDVPPPGFEHMTPQQYKALQASGQIPVNVYAAGQVPMPASTPNAPLTLNTNIPFAGSAVCRQARRLYVGNIPFTATEENMMDFFNKQMKAQNLVQAEGNPIIAVQINMEKNFAFLEFRSVDETTQGLALDGVVFNNQALKLRRPRDYAPLPGVSETPSIIVPGVVSTVVQDSPHKIFVGGLPNYLNEDQVKELLLTFGPLRGFNLVK 320
[Close]
BLAST of U2 snRNP auxiliary factor large subunit vs. TrEMBL
Match: A0A068Y9C9 (U2 snRNP auxiliary factor large subunit OS=Echinococcus multilocularis OX=6211 PE=3 SV=1)

HSP 1 Score: 262.307 bits (669), Expect = 2.776e-80
Identity = 127/210 (60.48%), Postives = 161/210 (76.67%), Query Frame = 1 
Query:  361 YKYWDVPPTGYEHMSPSEFKAMQAAGQVLPTIYAT-----ESGIVNTEQIMDQH-PSIGSAVSRQARRIYVGNIPLGVTEQGMMDFFNEQMKLKLFTQADGDPIIAVQINTDKNFAFLEFRSVEETSQGLNLDGIMYNGFPLKLRRPRDYVPLPTTMDT--MLPNSLAAGVVQDTPHKLFIGGLPHYLNEDQVKELLLTFGPLSGFNLVK 966
            YKYWDVPP G+EHM+P ++KA+QA+GQ+   +YA       +   N    ++ + P  GSAV RQARR+YVGNIP   TE+ MMDFFN+QMK +   QA+G+PIIAVQIN +KNFAFLEFRSV+ET+QGL LDGI+++   LKLRRPRDY PLP   +T  ++   + + VVQD+PHK+F+GGLP+YLNEDQVKELLL+FGPL GFNLVK
Sbjct:  110 YKYWDVPPPGFEHMTPQQYKALQASGQIPVNVYAAGQVPMPASTPNAPLTLNTNIPFAGSAVCRQARRLYVGNIPFTATEENMMDFFNKQMKAQNLVQAEGNPIIAVQINMEKNFAFLEFRSVDETTQGLALDGILFHNQALKLRRPRDYAPLPGVSETPSIIVPGVVSTVVQDSPHKIFVGGLPNYLNEDQVKELLLSFGPLKGFNLVK 319
[Close]
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Cavefish
Match: u2af2a (splicing factor U2AF 65 kDa subunit-like [Source:NCBI gene;Acc:103024792])

HSP 1 Score: 241.891 bits (616), Expect = 2.910e-76
Identity = 121/213 (56.81%), Postives = 154/213 (72.30%), Query Frame = 1 
Query:  367 YWDVPPTGYEHMSPSEFKAMQAAGQV-----LPTIYATESGIVNTEQIMDQHPSIGSAVSRQARRIYVGNIPLGVTEQGMMDFFNEQMKLKLFTQADGDPIIAVQINTDKNFAFLEFRSVEETSQGLNLDGIMYNGFPLKLRRPRDYVPLPTTMDT--------MLPNSLAAGVVQDTPHKLFIGGLPHYLNEDQVKELLLTFGPLSGFNLVK 966
            YWDVPP G+EH++P ++KAMQAAGQ+     LPT+  T  G+  T   +   P +GS ++RQARR+YVGNIP G+TE+ MMDFFN QM+L   TQA G+P++AVQIN DKNFAFLEFRSV+ET+Q +  DGI++ G  LK+RRP DY PLP   +          +P  + + VV D+ HKLFIGGLP+YLN+DQVKELL +FGPL  FNLVK
Sbjct:   81 YWDVPPPGFEHITPMQYKAMQAAGQIPATALLPTM--TPEGLAVTPTPV---PVVGSQMTRQARRLYVGNIPFGITEESMMDFFNAQMRLGGLTQAPGNPVLAVQINQDKNFAFLEFRSVDETTQAMAFDGIIFQGQSLKIRRPHDYQPLPGMSENPSVYVPGLSVPPGVVSTVVPDSAHKLFIGGLPNYLNDDQVKELLTSFGPLKAFNLVK 288
[Close]
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Cavefish
Match: u2af2b (splicing factor U2AF 65 kDa subunit [Source:NCBI gene;Acc:103026862])

HSP 1 Score: 240.736 bits (613), Expect = 6.651e-76
Identity = 120/207 (57.97%), Postives = 154/207 (74.40%), Query Frame = 1 
Query:  367 YWDVPPTGYEHMSPSEFKAMQAAGQV-----LPTIYATESGIVNTEQIMDQHPSIGSAVSRQARRIYVGNIPLGVTEQGMMDFFNEQMKLKLFTQADGDPIIAVQINTDKNFAFLEFRSVEETSQGLNLDGIMYNGFPLKLRRPRDYVPLPTTMD--TMLPNSLAAGVVQDTPHKLFIGGLPHYLNEDQVKELLLTFGPLSGFNLVK 966
            YWDVPP G+EH++P ++KAMQAAGQ+     LPT+  T  G+  T   +   P +GS ++RQARR+YVGNIP G+TE+ MMDFFN QM+L   TQA G+P++AVQIN DKNFAFLEFRSV+ET+Q +  DGI++ G  LK+RRP DY PLP   +  ++    + + VV D+ HKLFIGGLP+YLN+DQVKELL +FGPL  FNLVK
Sbjct:   75 YWDVPPPGFEHITPMQYKAMQAAGQIPATALLPTM--TPEGLAVTPTPV---PVVGSQMTRQARRLYVGNIPFGITEESMMDFFNAQMRLGGLTQAPGNPVLAVQINQDKNFAFLEFRSVDETTQAMAFDGIIFQGQSLKIRRPHDYQPLPGMSENPSVYVPGVVSTVVPDSVHKLFIGGLPNYLNDDQVKELLTSFGPLKAFNLVK 276
[Close]
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Cavefish
Match: u2af2b (splicing factor U2AF 65 kDa subunit [Source:NCBI gene;Acc:103026862])

HSP 1 Score: 240.736 bits (613), Expect = 7.096e-76
Identity = 120/207 (57.97%), Postives = 154/207 (74.40%), Query Frame = 1 
Query:  367 YWDVPPTGYEHMSPSEFKAMQAAGQV-----LPTIYATESGIVNTEQIMDQHPSIGSAVSRQARRIYVGNIPLGVTEQGMMDFFNEQMKLKLFTQADGDPIIAVQINTDKNFAFLEFRSVEETSQGLNLDGIMYNGFPLKLRRPRDYVPLPTTMD--TMLPNSLAAGVVQDTPHKLFIGGLPHYLNEDQVKELLLTFGPLSGFNLVK 966
            YWDVPP G+EH++P ++KAMQAAGQ+     LPT+  T  G+  T   +   P +GS ++RQARR+YVGNIP G+TE+ MMDFFN QM+L   TQA G+P++AVQIN DKNFAFLEFRSV+ET+Q +  DGI++ G  LK+RRP DY PLP   +  ++    + + VV D+ HKLFIGGLP+YLN+DQVKELL +FGPL  FNLVK
Sbjct:   82 YWDVPPPGFEHITPMQYKAMQAAGQIPATALLPTM--TPEGLAVTPTPV---PVVGSQMTRQARRLYVGNIPFGITEESMMDFFNAQMRLGGLTQAPGNPVLAVQINQDKNFAFLEFRSVDETTQAMAFDGIIFQGQSLKIRRPHDYQPLPGMSENPSVYVPGVVSTVVPDSVHKLFIGGLPNYLNDDQVKELLTSFGPLKAFNLVK 283
[Close]
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Sea Lamprey
Match: u2af2a (U2 small nuclear RNA auxiliary factor 2a [Source:ZFIN;Acc:ZDB-GENE-050706-131])

HSP 1 Score: 232.646 bits (592), Expect = 1.854e-73
Identity = 124/208 (59.62%), Postives = 152/208 (73.08%), Query Frame = 1 
Query:  370 WDVPPTGYEHMSPSEFKAMQAAGQV------LPTIYATESGIVNTEQIMDQHPSIGSAVSRQARRIYVGNIPLGVTEQGMMDFFNEQMKLKLFTQADGDPIIAVQINTDKNFAFLEFRSVEETSQGLNLDGIMYNGFPLKLRRPRDYVPLPTTMDT---MLPNSLAAGVVQDTPHKLFIGGLPHYLNEDQVKELLLTFGPLSGFNLVK 966
            WDVPP G+EH++P ++KAMQAAGQ+        T  AT +G+ N   +    P +GS ++RQARR+YVGNIP GVTE+ MMDFFN QMKL    QA G+P++AVQIN DKNFAFLEFRSV+ET+Q L  DGI++ G  LKLRRP DY PLP   ++    +P  + + VVQDTPHKLFIGGLP YL +DQVKELL +FGPL  FNLVK
Sbjct:   77 WDVPPPGFEHITPLQYKAMQAAGQIPATALIATTTTATVAGLPNPAAM--PVPIVGSQLTRQARRLYVGNIPFGVTEEAMMDFFNNQMKLAGLAQAPGNPVLAVQINLDKNFAFLEFRSVDETTQALAFDGIIFQGQSLKLRRPHDYQPLPGMSESPALHVPVGVVSTVVQDTPHKLFIGGLPSYLTDDQVKELLTSFGPLKAFNLVK 282
[Close]
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Nematostella
Match: EDO48329 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RJT7])

HSP 1 Score: 179.489 bits (454), Expect = 3.653e-54
Identity = 87/150 (58.00%), Postives = 112/150 (74.67%), Query Frame = 1 
Query:  526 VSRQARRIYVGNIPLGVTEQGMMDFFNEQMKLKLFTQADGDPIIAVQINTDKNFAFLEFRSVEETSQGLNLDGIMYNGFPLKLRRPRDYVPLPTTMDTM---LPNSLAAGVVQDTPHKLFIGGLPHYLNEDQVKELLLTFGPLSGFNLVK 966
            ++RQARR+YVGNIP GVTE  M++FFN +MK      A G+P+IA QINT++NFAF+E RSVEET+Q +  DGI+  G  LK+RRP+DY P+P   +     +P  ++  VV D+PHK+FIGGLP+YLNEDQVKELL +FG L  FNLVK
Sbjct:    1 MTRQARRLYVGNIPFGVTENLMIEFFNAKMKEAKLNTAPGNPVIAAQINTEQNFAFIELRSVEETTQAMAFDGIILQGQALKIRRPKDYQPIPGMSENASVHVPGVVST-VVPDSPHKIFIGGLPNYLNEDQVKELLSSFGELRAFNLVK 149
[Close]
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Medaka
Match: u2af2a (U2 small nuclear RNA auxiliary factor 2 [Source:NCBI gene;Acc:101165173])

HSP 1 Score: 241.121 bits (614), Expect = 2.382e-76
Identity = 120/207 (57.97%), Postives = 154/207 (74.40%), Query Frame = 1 
Query:  367 YWDVPPTGYEHMSPSEFKAMQAAGQV-----LPTIYATESGIVNTEQIMDQHPSIGSAVSRQARRIYVGNIPLGVTEQGMMDFFNEQMKLKLFTQADGDPIIAVQINTDKNFAFLEFRSVEETSQGLNLDGIMYNGFPLKLRRPRDYVPLPTTMD--TMLPNSLAAGVVQDTPHKLFIGGLPHYLNEDQVKELLLTFGPLSGFNLVK 966
            YWDVPP G+EH++P ++KAMQAAGQ+     LPT+  T  G+  T   +   P +GS ++RQARR+YVGNIP G+TE+ MMDFFN QM+L   TQA G+P++AVQIN DKNFAFLEFRSV+ET+Q +  DGI++ G  LK+RRP DY PLP   +  ++    + + VV D+ HKLFIGGLP+YLN+DQVKELL +FGPL  FNLVK
Sbjct:   59 YWDVPPPGFEHITPMQYKAMQAAGQIPATALLPTM--TPDGLAVTPTPV---PVVGSQMTRQARRLYVGNIPFGITEESMMDFFNAQMRLGGLTQAPGNPVLAVQINQDKNFAFLEFRSVDETTQAMAFDGIIFQGQSLKIRRPHDYQPLPGMSENPSVYVPGVVSTVVPDSAHKLFIGGLPNYLNDDQVKELLTSFGPLKAFNLVK 260
[Close]
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Medaka
Match: u2af2a (U2 small nuclear RNA auxiliary factor 2 [Source:NCBI gene;Acc:101165173])

HSP 1 Score: 241.506 bits (615), Expect = 4.538e-76
Identity = 120/207 (57.97%), Postives = 154/207 (74.40%), Query Frame = 1 
Query:  367 YWDVPPTGYEHMSPSEFKAMQAAGQV-----LPTIYATESGIVNTEQIMDQHPSIGSAVSRQARRIYVGNIPLGVTEQGMMDFFNEQMKLKLFTQADGDPIIAVQINTDKNFAFLEFRSVEETSQGLNLDGIMYNGFPLKLRRPRDYVPLPTTMD--TMLPNSLAAGVVQDTPHKLFIGGLPHYLNEDQVKELLLTFGPLSGFNLVK 966
            YWDVPP G+EH++P ++KAMQAAGQ+     LPT+  T  G+  T   +   P +GS ++RQARR+YVGNIP G+TE+ MMDFFN QM+L   TQA G+P++AVQIN DKNFAFLEFRSV+ET+Q +  DGI++ G  LK+RRP DY PLP   +  ++    + + VV D+ HKLFIGGLP+YLN+DQVKELL +FGPL  FNLVK
Sbjct:   90 YWDVPPPGFEHITPMQYKAMQAAGQIPATALLPTM--TPDGLAVTPTPV---PVVGSQMTRQARRLYVGNIPFGITEESMMDFFNAQMRLGGLTQAPGNPVLAVQINQDKNFAFLEFRSVDETTQAMAFDGIIFQGQSLKIRRPHDYQPLPGMSENPSVYVPGVVSTVVPDSAHKLFIGGLPNYLNDDQVKELLTSFGPLKAFNLVK 291
[Close]
BLAST of U2 snRNP auxiliary factor large subunit vs. Planmine SMEST
Match: SMESG000078029.1 (SMESG000078029.1)

HSP 1 Score: 468.774 bits (1205), Expect = 9.492e-165
Identity = 320/320 (100.00%), Postives = 320/320 (100.00%), Query Frame = 1 
Query:    7 MADEQTSSMIVXXXXXXXXXXXXXXXRIKHSDRFSNHEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXHIKTEPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXASVELKKDYKYWDVPPTGYEHMSPSEFKAMQAAGQVLPTIYATESGIVNTEQIMDQHPSIGSAVSRQARRIYVGNIPLGVTEQGMMDFFNEQMKLKLFTQADGDPIIAVQINTDKNFAFLEFRSVEETSQGLNLDGIMYNGFPLKLRRPRDYVPLPTTMDTMLPNSLAAGVVQDTPHKLFIGGLPHYLNEDQVKELLLTFGPLSGFNLVK 966
            MADEQTSSMIVSSSENNEHEVSGNSERIKHSDRFSNHEDSRRRDPRRDRDRSRSKRKHRSRSRERRRSRSKDRHIKTEPRRHDRSRERKKSRSRERKRSRSRNKRRDSRNASVELKKDYKYWDVPPTGYEHMSPSEFKAMQAAGQVLPTIYATESGIVNTEQIMDQHPSIGSAVSRQARRIYVGNIPLGVTEQGMMDFFNEQMKLKLFTQADGDPIIAVQINTDKNFAFLEFRSVEETSQGLNLDGIMYNGFPLKLRRPRDYVPLPTTMDTMLPNSLAAGVVQDTPHKLFIGGLPHYLNEDQVKELLLTFGPLSGFNLVK
Sbjct:    1 MADEQTSSMIVSSSENNEHEVSGNSERIKHSDRFSNHEDSRRRDPRRDRDRSRSKRKHRSRSRERRRSRSKDRHIKTEPRRHDRSRERKKSRSRERKRSRSRNKRRDSRNASVELKKDYKYWDVPPTGYEHMSPSEFKAMQAAGQVLPTIYATESGIVNTEQIMDQHPSIGSAVSRQARRIYVGNIPLGVTEQGMMDFFNEQMKLKLFTQADGDPIIAVQINTDKNFAFLEFRSVEETSQGLNLDGIMYNGFPLKLRRPRDYVPLPTTMDTMLPNSLAAGVVQDTPHKLFIGGLPHYLNEDQVKELLLTFGPLSGFNLVK 320
[Close]
BLAST of U2 snRNP auxiliary factor large subunit vs. Planmine SMEST
Match: SMESG000018652.1 (SMESG000018652.1)

HSP 1 Score: 337.806 bits (865), Expect = 9.696e-114
Identity = 156/208 (75.00%), Postives = 180/208 (86.54%), Query Frame = 1 
Query:  343 VELKKDYKYWDVPPTGYEHMSPSEFKAMQAAGQVLPTIYATESGIVNTEQIMDQHPSIGSAVSRQARRIYVGNIPLGVTEQGMMDFFNEQMKLKLFTQADGDPIIAVQINTDKNFAFLEFRSVEETSQGLNLDGIMYNGFPLKLRRPRDYVPLPTTMDTMLPNSLAAGVVQDTPHKLFIGGLPHYLNEDQVKELLLTFGPLSGFNLVK 966
            +ELKK+YKYWDV PTGYE+MS +EFK + + GQVLP++Y TE  +VN      ++PSIG+ VSRQARRIYVGN+PLGVTE+GMM+FFNEQ+KLK  TQADGDPIIAVQINTDKNFAFLEFRSVEET+QGLNLDGI+Y+GF LKLRRPRDYVPLP   D ++ NS+ AG+VQDTPHKLF+GGLP YL EDQVKELL TFGPL GFNLVK
Sbjct:   92 LELKKNYKYWDVAPTGYENMSAAEFKQIHSGGQVLPSLYLTEGNVVNMGLTASEYPSIGNTVSRQARRIYVGNLPLGVTEEGMMEFFNEQIKLKELTQADGDPIIAVQINTDKNFAFLEFRSVEETNQGLNLDGIVYHGFALKLRRPRDYVPLPQAFDGIVSNSMTAGIVQDTPHKLFVGGLPQYLGEDQVKELLQTFGPLRGFNLVK 299
[Close]
BLAST of U2 snRNP auxiliary factor large subunit vs. Planmine SMEST
Match: SMESG000018647.1 (SMESG000018647.1)

HSP 1 Score: 337.806 bits (865), Expect = 9.696e-114
Identity = 156/208 (75.00%), Postives = 180/208 (86.54%), Query Frame = 1 
Query:  343 VELKKDYKYWDVPPTGYEHMSPSEFKAMQAAGQVLPTIYATESGIVNTEQIMDQHPSIGSAVSRQARRIYVGNIPLGVTEQGMMDFFNEQMKLKLFTQADGDPIIAVQINTDKNFAFLEFRSVEETSQGLNLDGIMYNGFPLKLRRPRDYVPLPTTMDTMLPNSLAAGVVQDTPHKLFIGGLPHYLNEDQVKELLLTFGPLSGFNLVK 966
            +ELKK+YKYWDV PTGYE+MS +EFK + + GQVLP++Y TE  +VN      ++PSIG+ VSRQARRIYVGN+PLGVTE+GMM+FFNEQ+KLK  TQADGDPIIAVQINTDKNFAFLEFRSVEET+QGLNLDGI+Y+GF LKLRRPRDYVPLP   D ++ NS+ AG+VQDTPHKLF+GGLP YL EDQVKELL TFGPL GFNLVK
Sbjct:   92 LELKKNYKYWDVAPTGYENMSAAEFKQIHSGGQVLPSLYLTEGNVVNMGLTASEYPSIGNTVSRQARRIYVGNLPLGVTEEGMMEFFNEQIKLKELTQADGDPIIAVQINTDKNFAFLEFRSVEETNQGLNLDGIVYHGFALKLRRPRDYVPLPQAFDGIVSNSMTAGIVQDTPHKLFVGGLPQYLGEDQVKELLQTFGPLRGFNLVK 299
[Close]
BLAST of U2 snRNP auxiliary factor large subunit vs. Planmine SMEST
Match: SMESG000051274.1 (SMESG000051274.1)

HSP 1 Score: 254.988 bits (650), Expect = 1.127e-81
Identity = 129/219 (58.90%), Postives = 161/219 (73.52%), Query Frame = 1 
Query:  340 SVELKKDYKYWDVPPTGYEHMSPSEFKAMQAAGQVLPTIYATESGIV-------NTEQIMDQH-PSIGSAVSRQARRIYVGNIPLGVTEQGMMDFFNEQMKLKLFTQADGDPIIAVQINTDKNFAFLEFRSVEETSQGLNLDGIMYNGFPLKLRRPRDYVPLPTTMDT--MLPNSLAAGVVQDTPHKLFIGGLPHYLNEDQVKELLLTFGPLSGFNLVK 966
            S+  KK YKYWDVPP G++H+SP+ +KAMQA+GQ+   +YA  +G V       N   ++    P  GSAV RQARR+YVGNIP G+TE  M++ FN QMK+K   QA+GDP+IAVQIN DKNFAF+EFRSV+ET+Q L LDGI + G  LKLRRPRDYVPLP   D   M    + + +VQ+TP+K+FIG LP+YL +DQVKELLL+FGPL GFNLVK
Sbjct:   58 SLPSKKAYKYWDVPPQGFDHISPAHYKAMQASGQIPINVYA--AGQVPMPDNAPNAPLVLHSDIPFAGSAVCRQARRLYVGNIPFGITETAMIELFNSQMKMKGLMQAEGDPVIAVQINIDKNFAFVEFRSVDETTQALALDGINFQGQQLKLRRPRDYVPLPGLSDNPQMSVPGVVSTMVQETPNKVFIGSLPNYLTDDQVKELLLSFGPLRGFNLVK 274
The following BLAST results are available for this feature:
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 4
Match NameE-valueIdentityDescription
U2AF21.920e-7758.02U2 small nuclear RNA auxiliary factor 2 [Source:HG... [more]
U2AF22.362e-7758.02U2 small nuclear RNA auxiliary factor 2 [Source:HG... [more]
U2AF22.377e-4762.31U2 small nuclear RNA auxiliary factor 2 [Source:HG... [more]
U2AF21.341e-2053.66U2 small nuclear RNA auxiliary factor 2 [Source:HG... [more]
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BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 3
Match NameE-valueIdentityDescription
uaf-11.163e-6551.96Splicing factor U2AF 65 kDa subunit [Source:UniPr... [more]
uaf-11.418e-6551.96Splicing factor U2AF 65 kDa subunit [Source:UniPr... [more]
uaf-12.358e-6551.96Splicing factor U2AF 65 kDa subunit [Source:UniPr... [more]
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BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
U2af501.298e-7454.50gene:FBgn0005411 transcript:FBtr0307178[more]
U2af501.298e-7454.50gene:FBgn0005411 transcript:FBtr0074234[more]
U2af502.168e-7454.50gene:FBgn0005411 transcript:FBtr0307176[more]
U2af501.511e-6454.45gene:FBgn0005411 transcript:FBtr0307177[more]
LS29.909e-5442.99gene:FBgn0034834 transcript:FBtr0072052[more]
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BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
u2af2a2.818e-7758.49U2 small nuclear RNA auxiliary factor 2a [Source:Z... [more]
u2af2a6.059e-7657.97U2 small nuclear RNA auxiliary factor 2a [Source:Z... [more]
u2af2b6.342e-7557.49U2 small nuclear RNA auxiliary factor 2b [Source:Z... [more]
u2af2b4.692e-7456.60U2 small nuclear RNA auxiliary factor 2b [Source:Z... [more]
u2af2b5.236e-5753.04U2 small nuclear RNA auxiliary factor 2b [Source:Z... [more]
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BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 3
Match NameE-valueIdentityDescription
mbp4.494e-7858.02myelin basic protein [Source:Xenbase;Acc:XB-GENE-9... [more]
mbp1.556e-7758.02myelin basic protein [Source:Xenbase;Acc:XB-GENE-9... [more]
mbp2.911e-7657.08myelin basic protein [Source:Xenbase;Acc:XB-GENE-9... [more]
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BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 4
Match NameE-valueIdentityDescription
U2af21.361e-7758.02U2 small nuclear ribonucleoprotein auxiliary facto... [more]
U2af21.622e-7758.02U2 small nuclear ribonucleoprotein auxiliary facto... [more]
U2af21.632e-4762.31U2 small nuclear ribonucleoprotein auxiliary facto... [more]
U2af21.465e-2053.66U2 small nuclear ribonucleoprotein auxiliary facto... [more]
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BLAST of U2 snRNP auxiliary factor large subunit vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P26368|U2AF2_HUMAN9.221e-7758.02Splicing factor U2AF 65 kDa subunit OS=Homo sapien... [more]
sp|P26369|U2AF2_MOUSE9.523e-7758.02Splicing factor U2AF 65 kDa subunit OS=Mus musculu... [more]
sp|Q24562|U2AF2_DROME1.301e-7354.50Splicing factor U2AF 50 kDa subunit OS=Drosophila ... [more]
sp|P90978|U2AF2_CAEEL3.000e-6451.96Splicing factor U2AF 65 kDa subunit OS=Caenorhabdi... [more]
sp|P90727|U2AF2_CAEBR4.207e-6450.99Splicing factor U2AF 65 kDa subunit OS=Caenorhabdi... [more]
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BLAST of U2 snRNP auxiliary factor large subunit vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0L8G0Y04.584e-8461.06U2 snRNP auxiliary factor large subunit OS=Octopus... [more]
A0A0L8G0P84.377e-8361.06U2 snRNP auxiliary factor large subunit OS=Octopus... [more]
V4A7622.368e-8161.90U2 snRNP auxiliary factor large subunit OS=Lottia ... [more]
A0A0R3U1R13.323e-8160.95U2 snRNP auxiliary factor large subunit OS=Mesoces... [more]
A0A068Y9C92.776e-8060.48U2 snRNP auxiliary factor large subunit OS=Echinoc... [more]
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BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 3
Match NameE-valueIdentityDescription
u2af2a2.910e-7656.81splicing factor U2AF 65 kDa subunit-like [Source:N... [more]
u2af2b6.651e-7657.97splicing factor U2AF 65 kDa subunit [Source:NCBI g... [more]
u2af2b7.096e-7657.97splicing factor U2AF 65 kDa subunit [Source:NCBI g... [more]
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BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
u2af2a1.854e-7359.62U2 small nuclear RNA auxiliary factor 2a [Source:Z... [more]
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BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO483293.653e-5458.00Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2
Match NameE-valueIdentityDescription
u2af2a2.382e-7657.97U2 small nuclear RNA auxiliary factor 2 [Source:NC... [more]
u2af2a4.538e-7657.97U2 small nuclear RNA auxiliary factor 2 [Source:NC... [more]
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BLAST of U2 snRNP auxiliary factor large subunit vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 4
Match NameE-valueIdentityDescription
SMESG000078029.19.492e-165100.00SMESG000078029.1[more]
SMESG000018652.19.696e-11475.00SMESG000018652.1[more]
SMESG000018647.19.696e-11475.00SMESG000018647.1[more]
SMESG000051274.11.127e-8158.90SMESG000051274.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30028920 ID=SMED30028920|Name=U2 snRNP auxiliary factor large subunit|organism=Schmidtea mediterranea sexual|type=transcript|length=967bp
TTTTAGATGGCTGATGAGCAAACTAGTTCTATGATAGTTTCATCTAGTGA
AAATAATGAGCATGAAGTATCAGGGAACTCTGAACGAATAAAGCATTCTG
ACAGATTTTCAAATCATGAAGATAGTAGACGACGAGATCCTAGGAGAGAC
CGTGATCGTTCACGAAGTAAAAGGAAACATAGATCTCGAAGCCGAGAAAG
AAGACGTTCGAGGTCAAAAGACAGACATATCAAGACTGAACCTAGAAGAC
ACGATAGGAGTAGAGAACGTAAAAAATCTAGAAGCCGCGAGAGGAAAAGG
TCCCGTAGTCGTAATAAAAGAAGAGATTCGCGAAATGCATCTGTTGAACT
GAAAAAAGATTACAAATATTGGGATGTTCCTCCAACTGGATATGAACACA
TGTCCCCATCCGAATTTAAAGCCATGCAAGCGGCTGGTCAAGTTTTACCA
ACAATTTATGCAACTGAGAGTGGGATTGTTAATACAGAACAAATAATGGA
TCAACATCCGTCAATCGGTAGTGCAGTTAGTCGACAAGCTCGAAGAATAT
ATGTTGGAAATATTCCGCTGGGAGTTACTGAACAAGGAATGATGGATTTC
TTCAATGAACAAATGAAATTAAAATTATTCACCCAAGCTGATGGAGATCC
CATTATCGCTGTACAAATAAATACTGATAAAAATTTTGCTTTTTTAGAAT
TCAGGTCAGTTGAAGAAACAAGTCAAGGTTTAAATTTAGATGGTATTATG
TATAATGGGTTTCCATTGAAATTGAGACGTCCACGTGATTATGTTCCTTT
ACCTACTACGATGGACACAATGTTGCCGAATTCATTAGCAGCTGGGGTTG
TTCAAGATACACCTCATAAATTATTCATTGGCGGCTTGCCACATTATTTA
AATGAAGATCAAGTGAAGGAACTTCTACTAACATTCGGTCCACTGAGTGG
TTTCAATTTAGTAAAGG
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protein sequence of SMED30028920-orf-1

>SMED30028920-orf-1 ID=SMED30028920-orf-1|Name=SMED30028920-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=320bp
MADEQTSSMIVSSSENNEHEVSGNSERIKHSDRFSNHEDSRRRDPRRDRD
RSRSKRKHRSRSRERRRSRSKDRHIKTEPRRHDRSRERKKSRSRERKRSR
SRNKRRDSRNASVELKKDYKYWDVPPTGYEHMSPSEFKAMQAAGQVLPTI
YATESGIVNTEQIMDQHPSIGSAVSRQARRIYVGNIPLGVTEQGMMDFFN
EQMKLKLFTQADGDPIIAVQINTDKNFAFLEFRSVEETSQGLNLDGIMYN
GFPLKLRRPRDYVPLPTTMDTMLPNSLAAGVVQDTPHKLFIGGLPHYLNE
DQVKELLLTFGPLSGFNLVK
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: biological process
TermDefinition
GO:0008380RNA splicing
GO:0006397mRNA processing
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002111X2 cell
Vocabulary: INTERPRO
TermDefinition
IPR035979RBD_domain_sf
IPR000504RRM_dom
IPR012677Nucleotide-bd_a/b_plait_sf
Vocabulary: molecular function
TermDefinition
GO:0003676nucleic acid binding
GO:0000166nucleotide binding
GO:0003723RNA binding
Vocabulary: cellular component
TermDefinition
GO:0005634nucleus
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000504RNA recognition motif domainSMARTSM00360rrm1_1coord: 180..257
e-value: 3.7E-8
score: 43.1
IPR000504RNA recognition motif domainPFAMPF00076RRM_1coord: 181..255
e-value: 2.2E-4
score: 21.0
IPR000504RNA recognition motif domainPROSITEPS50102RRMcoord: 179..261
score: 12.987
IPR012677Nucleotide-binding alpha-beta plait domain superfamilyGENE3DG3DSA:3.30.70.330coord: 265..320
e-value: 1.1E-36
score: 128.3
IPR012677Nucleotide-binding alpha-beta plait domain superfamilyGENE3DG3DSA:3.30.70.330coord: 179..264
e-value: 1.1E-36
score: 128.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..112
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 85..105
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..19
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 20..47
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 48..75
NoneNo IPR availablePANTHERPTHR23139RNA-BINDING PROTEINcoord: 14..320
NoneNo IPR availablePANTHERPTHR23139:SF9SPLICING FACTOR U2AF 65 KDA SUBUNITcoord: 14..320
NoneNo IPR availableCDDcd12230RRM1_U2AF65coord: 178..259
e-value: 5.04577E-44
score: 143.45
IPR035979RNA-binding domain superfamilySUPERFAMILYSSF54928RNA-binding domain, RBDcoord: 177..318