GTP cyclohydrolase 1 feedback regulatory protein

Overview
NameGTP cyclohydrolase 1 feedback regulatory protein
Smed IDSMED30028908
Length (bp)439
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of GTP cyclohydrolase 1 feedback regulatory protein (SMED30028908) t-SNE clustered cells

Violin plots show distribution of expression levels for GTP cyclohydrolase 1 feedback regulatory protein (SMED30028908) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of GTP cyclohydrolase 1 feedback regulatory protein (SMED30028908) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for GTP cyclohydrolase 1 feedback regulatory protein (SMED30028908) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30028908

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 13

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30028908SMESG000042387.1 Contig1419newmark_estsPMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30028908SMESG000042387.1 Contig1419uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30028908SMESG000072323.1 Contig1419newmark_estsPMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30028908SMESG000072323.1 Contig1419uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
head regionSMED30028908SMESG000042387.1 SmedASXL_006018SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
protonephridiaSMED30028908SMESG000042387.1 dd_Smed_v4_6329_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30028908SMESG000042387.1 dd_Smed_v4_6329_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30028908SMESG000042387.1 dd_Smed_v4_6329_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30028908SMESG000042387.1 Contig1419newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30028908SMESG000042387.1 Contig1419uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30028908SMESG000072323.1 Contig1419newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30028908SMESG000072323.1 Contig1419uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
head regionSMED30028908SMESG000042387.1 dd_Smed_v6_6329_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of GTP cyclohydrolase 1 feedback regulatory protein vs. Ensembl Human
Match: GCHFR (GTP cyclohydrolase I feedback regulator [Source:HGNC Symbol;Acc:HGNC:4194])

HSP 1 Score: 83.1889 bits (204), Expect = 2.850e-21
Identity = 37/67 (55.22%), Postives = 51/67 (76.12%), Query Frame = 1 
Query:  118 FVLISTQVRLESGPTYVGDEESDPELMTYLNAEKIKALGNNFSHYVSKLKPREIMDLLDKKGFSLIS 318
            ++LISTQ+R+E GPT VGDE+SDPELM +L A K +ALGNNF  Y     PR ++D L+++GF ++S
Sbjct:    3 YLLISTQIRMEVGPTMVGDEQSDPELMQHLGASKRRALGNNFYEYYVDDPPRIVLDKLERRGFRVLS 69
[Close]
BLAST of GTP cyclohydrolase 1 feedback regulatory protein vs. Ensembl Human
Match: GCHFR (GTP cyclohydrolase I feedback regulator [Source:HGNC Symbol;Acc:HGNC:4194])

HSP 1 Score: 65.0846 bits (157), Expect = 1.155e-14
Identity = 28/42 (66.67%), Postives = 35/42 (83.33%), Query Frame = 1 
Query:  118 FVLISTQVRLESGPTYVGDEESDPELMTYLNAEKIKALGNNF 243
            ++LISTQ+R+E GPT VGDE+SDPELM +L A K +ALGNN 
Sbjct:    3 YLLISTQIRMEVGPTMVGDEQSDPELMQHLGASKRRALGNNL 44
[Close]
BLAST of GTP cyclohydrolase 1 feedback regulatory protein vs. Ensembl Human
Match: GCHFR (GTP cyclohydrolase I feedback regulator [Source:HGNC Symbol;Acc:HGNC:4194])

HSP 1 Score: 60.4622 bits (145), Expect = 1.207e-12
Identity = 27/51 (52.94%), Postives = 37/51 (72.55%), Query Frame = 1 
Query:  166 VGDEESDPELMTYLNAEKIKALGNNFSHYVSKLKPREIMDLLDKKGFSLIS 318
            VGDE+SDPELM +L A K +ALGNNF  Y     PR ++D L+++GF ++S
Sbjct:    2 VGDEQSDPELMQHLGASKRRALGNNFYEYYVDDPPRIVLDKLERRGFRVLS 52
[Close]
BLAST of GTP cyclohydrolase 1 feedback regulatory protein vs. Ensembl Human
Match: GCHFR (GTP cyclohydrolase I feedback regulator [Source:HGNC Symbol;Acc:HGNC:4194])

HSP 1 Score: 60.4622 bits (145), Expect = 1.207e-12
Identity = 27/51 (52.94%), Postives = 37/51 (72.55%), Query Frame = 1 
Query:  166 VGDEESDPELMTYLNAEKIKALGNNFSHYVSKLKPREIMDLLDKKGFSLIS 318
            VGDE+SDPELM +L A K +ALGNNF  Y     PR ++D L+++GF ++S
Sbjct:    2 VGDEQSDPELMQHLGASKRRALGNNFYEYYVDDPPRIVLDKLERRGFRVLS 52
[Close]
BLAST of GTP cyclohydrolase 1 feedback regulatory protein vs. Ensembl Human
Match: GCHFR (GTP cyclohydrolase I feedback regulator [Source:HGNC Symbol;Acc:HGNC:4194])

HSP 1 Score: 57.3806 bits (137), Expect = 2.043e-11
Identity = 29/67 (43.28%), Postives = 42/67 (62.69%), Query Frame = 1 
Query:  118 FVLISTQVRLESGPTYVGDEESDPELMTYLNAEKIKALGNNFSHYVSKLKPREIMDLLDKKGFSLIS 318
            ++LISTQ+R+           SDPELM +L A K +ALGNNF  Y     PR ++D L+++GF ++S
Sbjct:    3 YLLISTQIRM-----------SDPELMQHLGASKRRALGNNFYEYYVDDPPRIVLDKLERRGFRVLS 58
[Close]
BLAST of GTP cyclohydrolase 1 feedback regulatory protein vs. Ensembl Celegans
Match: gfrp-1 (GTP cyclohydrolase I Feedback Regulatory Protein homolog [Source:UniProtKB/TrEMBL;Acc:D6RYD9])

HSP 1 Score: 82.0333 bits (201), Expect = 3.681e-21
Identity = 33/67 (49.25%), Postives = 52/67 (77.61%), Query Frame = 1 
Query:  118 FVLISTQVRLESGPTYVGDEESDPELMTYLNAEKIKALGNNFSHYVSKLKPREIMDLLDKKGFSLIS 318
            ++LISTQ+R+E GPT VGD ESDP+LM  L A+  K LGN F  Y++K  PR++++ L+K+G+++++
Sbjct:    3 YILISTQIRMECGPTMVGDGESDPKLMGTLEAKLTKQLGNEFQEYITKWSPRKVLNQLEKEGWTVVA 69
[Close]
BLAST of GTP cyclohydrolase 1 feedback regulatory protein vs. Ensembl Zebrafish
Match: gchfr (GTP cyclohydrolase I feedback regulator [Source:NCBI gene;Acc:393735])

HSP 1 Score: 85.1149 bits (209), Expect = 3.314e-22
Identity = 39/67 (58.21%), Postives = 49/67 (73.13%), Query Frame = 1 
Query:  118 FVLISTQVRLESGPTYVGDEESDPELMTYLNAEKIKALGNNFSHYVSKLKPREIMDLLDKKGFSLIS 318
            ++LISTQ+RLE+GPT VGDE SDP +M YL A KI  LGNNFS Y     PR ++D LDK G+ ++S
Sbjct:    3 YILISTQIRLETGPTMVGDEYSDPSIMNYLGARKITVLGNNFSEYHVDEPPRLVLDKLDKIGYRVVS 69
[Close]
BLAST of GTP cyclohydrolase 1 feedback regulatory protein vs. Ensembl Xenopus
Match: eci2 (enoyl-CoA delta isomerase 2 [Source:Xenbase;Acc:XB-GENE-961246])

HSP 1 Score: 74.7146 bits (182), Expect = 5.210e-18
Identity = 35/67 (52.24%), Postives = 47/67 (70.15%), Query Frame = 1 
Query:  118 FVLISTQVRLESGPTYVGDEESDPELMTYLNAEKIKALGNNFSHYVSKLKPREIMDLLDKKGFSLIS 318
            +VLISTQ+R+E+GPT VGDE SD +LM  L A+K   LGNNFS Y     PR  ++ L+K G+ ++S
Sbjct:    3 YVLISTQIRMETGPTIVGDEFSDTQLMAQLEADKRTVLGNNFSEYCVNEPPRVTLNKLEKLGYRVVS 69
[Close]
BLAST of GTP cyclohydrolase 1 feedback regulatory protein vs. Ensembl Mouse
Match: Gchfr (GTP cyclohydrolase I feedback regulator [Source:MGI Symbol;Acc:MGI:2443977])

HSP 1 Score: 78.9518 bits (193), Expect = 9.305e-20
Identity = 37/67 (55.22%), Postives = 47/67 (70.15%), Query Frame = 1 
Query:  118 FVLISTQVRLESGPTYVGDEESDPELMTYLNAEKIKALGNNFSHYVSKLKPREIMDLLDKKGFSLIS 318
            ++LISTQ+R+E GPT VGDE SDPELM +L A K   LGNNF  Y     PR ++D L+ KGF ++S
Sbjct:    3 YLLISTQIRMEVGPTMVGDEHSDPELMQHLGASKRSVLGNNFYEYYVNDPPRIVLDKLECKGFRVLS 69
[Close]
BLAST of GTP cyclohydrolase 1 feedback regulatory protein vs. UniProt/SwissProt
Match: sp|Q6PBT6|GFRP_DANRE (GTP cyclohydrolase 1 feedback regulatory protein OS=Danio rerio OX=7955 GN=gchfr PE=3 SV=1)

HSP 1 Score: 85.1149 bits (209), Expect = 2.502e-21
Identity = 39/67 (58.21%), Postives = 49/67 (73.13%), Query Frame = 1 
Query:  118 FVLISTQVRLESGPTYVGDEESDPELMTYLNAEKIKALGNNFSHYVSKLKPREIMDLLDKKGFSLIS 318
            ++LISTQ+RLE+GPT VGDE SDP +M YL A KI  LGNNFS Y     PR ++D LDK G+ ++S
Sbjct:    3 YILISTQIRLETGPTMVGDEYSDPSIMNYLGARKITVLGNNFSEYHVDEPPRLVLDKLDKIGYRVVS 69
[Close]
BLAST of GTP cyclohydrolase 1 feedback regulatory protein vs. UniProt/SwissProt
Match: sp|P30047|GFRP_HUMAN (GTP cyclohydrolase 1 feedback regulatory protein OS=Homo sapiens OX=9606 GN=GCHFR PE=1 SV=3)

HSP 1 Score: 83.1889 bits (204), Expect = 1.369e-20
Identity = 37/67 (55.22%), Postives = 51/67 (76.12%), Query Frame = 1 
Query:  118 FVLISTQVRLESGPTYVGDEESDPELMTYLNAEKIKALGNNFSHYVSKLKPREIMDLLDKKGFSLIS 318
            ++LISTQ+R+E GPT VGDE+SDPELM +L A K +ALGNNF  Y     PR ++D L+++GF ++S
Sbjct:    3 YLLISTQIRMEVGPTMVGDEQSDPELMQHLGASKRRALGNNFYEYYVDDPPRIVLDKLERRGFRVLS 69
[Close]
BLAST of GTP cyclohydrolase 1 feedback regulatory protein vs. UniProt/SwissProt
Match: sp|Q32L41|GFRP_BOVIN (GTP cyclohydrolase 1 feedback regulatory protein OS=Bos taurus OX=9913 GN=GCHFR PE=3 SV=1)

HSP 1 Score: 82.0333 bits (201), Expect = 4.278e-20
Identity = 37/67 (55.22%), Postives = 49/67 (73.13%), Query Frame = 1 
Query:  118 FVLISTQVRLESGPTYVGDEESDPELMTYLNAEKIKALGNNFSHYVSKLKPREIMDLLDKKGFSLIS 318
            ++LISTQ+R+E GPT VGDE SDPELM +L A K   LGNNFS Y     PR ++D L+++GF ++S
Sbjct:    3 YLLISTQIRMEVGPTVVGDEHSDPELMQHLGASKRSVLGNNFSEYYVNDPPRIVLDKLERRGFRVLS 69
[Close]
BLAST of GTP cyclohydrolase 1 feedback regulatory protein vs. UniProt/SwissProt
Match: sp|P99025|GFRP_MOUSE (GTP cyclohydrolase 1 feedback regulatory protein OS=Mus musculus OX=10090 GN=Gchfr PE=1 SV=3)

HSP 1 Score: 78.9518 bits (193), Expect = 6.508e-19
Identity = 37/67 (55.22%), Postives = 47/67 (70.15%), Query Frame = 1 
Query:  118 FVLISTQVRLESGPTYVGDEESDPELMTYLNAEKIKALGNNFSHYVSKLKPREIMDLLDKKGFSLIS 318
            ++LISTQ+R+E GPT VGDE SDPELM +L A K   LGNNF  Y     PR ++D L+ KGF ++S
Sbjct:    3 YLLISTQIRMEVGPTMVGDEHSDPELMQHLGASKRSVLGNNFYEYYVNDPPRIVLDKLECKGFRVLS 69
[Close]
BLAST of GTP cyclohydrolase 1 feedback regulatory protein vs. UniProt/SwissProt
Match: sp|P70552|GFRP_RAT (GTP cyclohydrolase 1 feedback regulatory protein OS=Rattus norvegicus OX=10116 GN=Gchfr PE=1 SV=3)

HSP 1 Score: 78.1814 bits (191), Expect = 1.375e-18
Identity = 36/67 (53.73%), Postives = 47/67 (70.15%), Query Frame = 1 
Query:  118 FVLISTQVRLESGPTYVGDEESDPELMTYLNAEKIKALGNNFSHYVSKLKPREIMDLLDKKGFSLIS 318
            ++LISTQ+R+E GPT VGDE SDPELM  L A K + LGNNF  Y     PR ++D L+ +GF ++S
Sbjct:    3 YLLISTQIRMEVGPTMVGDEHSDPELMQQLGASKRRVLGNNFYEYYVNDPPRIVLDKLECRGFRVLS 69
[Close]
BLAST of GTP cyclohydrolase 1 feedback regulatory protein vs. TrEMBL
Match: A0A433TTT0 (Uncharacterized protein OS=Elysia chlorotica OX=188477 GN=EGW08_007275 PE=4 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 8.910e-21
Identity = 39/67 (58.21%), Postives = 53/67 (79.10%), Query Frame = 1 
Query:  118 FVLISTQVRLESGPTYVGDEESDPELMTYLNAEKIKALGNNFSHYVSKLKPREIMDLLDKKGFSLIS 318
            +++IS QVRL+ GPT+VGDE SDPELM +LNA  IK LGNN++ Y + L PR++MD L+ KGF L++
Sbjct:    3 YIIISIQVRLDVGPTFVGDEWSDPELMEFLNASLIKQLGNNYAQYKTDLPPRQVMDRLETKGFKLVA 69
[Close]
BLAST of GTP cyclohydrolase 1 feedback regulatory protein vs. TrEMBL
Match: A0A401P3Z4 (Uncharacterized protein OS=Scyliorhinus torazame OX=75743 GN=scyTo_0013745 PE=4 SV=1)

HSP 1 Score: 88.5817 bits (218), Expect = 3.298e-20
Identity = 43/67 (64.18%), Postives = 51/67 (76.12%), Query Frame = 1 
Query:  118 FVLISTQVRLESGPTYVGDEESDPELMTYLNAEKIKALGNNFSHYVSKLKPREIMDLLDKKGFSLIS 318
            +VLISTQ+RLE+GPT VGDE SD ELM YL AEK  ALGNNFS Y  K  PR ++D L+  G+S+IS
Sbjct:    3 YVLISTQIRLETGPTIVGDEHSDEELMRYLGAEKTTALGNNFSEYRVKYPPRMVLDKLEIIGYSVIS 69
[Close]
BLAST of GTP cyclohydrolase 1 feedback regulatory protein vs. TrEMBL
Match: Q4RLP3 (Chromosome 10 SCAF15019, whole genome shotgun sequence (Fragment) OS=Tetraodon nigroviridis OX=99883 GN=GSTENG00032398001 PE=4 SV=1)

HSP 1 Score: 85.5001 bits (210), Expect = 4.961e-19
Identity = 40/67 (59.70%), Postives = 50/67 (74.63%), Query Frame = 1 
Query:  118 FVLISTQVRLESGPTYVGDEESDPELMTYLNAEKIKALGNNFSHYVSKLKPREIMDLLDKKGFSLIS 318
            ++LISTQ+RLE+GPT VGDE SDP++MTYL A K   LGNNFS Y     PR ++D L+K GF L+S
Sbjct:    3 YMLISTQIRLENGPTNVGDEHSDPQVMTYLGARKTTVLGNNFSEYYVDDPPRLVLDKLEKIGFRLLS 69
[Close]
BLAST of GTP cyclohydrolase 1 feedback regulatory protein vs. TrEMBL
Match: H3DJA1 (GTP cyclohydrolase I feedback regulator OS=Tetraodon nigroviridis OX=99883 PE=4 SV=1)

HSP 1 Score: 85.5001 bits (210), Expect = 5.042e-19
Identity = 40/67 (59.70%), Postives = 50/67 (74.63%), Query Frame = 1 
Query:  118 FVLISTQVRLESGPTYVGDEESDPELMTYLNAEKIKALGNNFSHYVSKLKPREIMDLLDKKGFSLIS 318
            ++LISTQ+RLE+GPT VGDE SDP++MTYL A K   LGNNFS Y     PR ++D L+K GF L+S
Sbjct:    3 YMLISTQIRLENGPTNVGDEHSDPQVMTYLGARKTTVLGNNFSEYYVDDPPRLVLDKLEKIGFRLLS 69
[Close]
BLAST of GTP cyclohydrolase 1 feedback regulatory protein vs. TrEMBL
Match: M1ERP8 (GTP cyclohydrolase I feedback regulator (Fragment) OS=Mustela putorius furo OX=9669 PE=2 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 9.115e-19
Identity = 39/67 (58.21%), Postives = 49/67 (73.13%), Query Frame = 1 
Query:  118 FVLISTQVRLESGPTYVGDEESDPELMTYLNAEKIKALGNNFSHYVSKLKPREIMDLLDKKGFSLIS 318
            +VLISTQ+R+E GPT VGDE SDPELM YL A K  ALGNNF  Y     PR ++D L+++GF ++S
Sbjct:    3 YVLISTQIRMEVGPTMVGDEHSDPELMQYLGASKRSALGNNFYEYFVSDPPRIVLDKLERRGFRVLS 69
[Close]
BLAST of GTP cyclohydrolase 1 feedback regulatory protein vs. Ensembl Cavefish
Match: gchfr (GTP cyclohydrolase I feedback regulator [Source:NCBI gene;Acc:393735])

HSP 1 Score: 55.0694 bits (131), Expect = 1.003e-10
Identity = 25/51 (49.02%), Postives = 34/51 (66.67%), Query Frame = 1 
Query:  166 VGDEESDPELMTYLNAEKIKALGNNFSHYVSKLKPREIMDLLDKKGFSLIS 318
            VGDE SDP +M+YL A K   LGNNFS Y     PR ++D L+K G+ +++
Sbjct:    2 VGDEFSDPSIMSYLGARKTTMLGNNFSEYHVDEPPRMVLDKLEKIGYKVVT 52
[Close]
BLAST of GTP cyclohydrolase 1 feedback regulatory protein vs. Ensembl Nematostella
Match: EDO32067 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SW52])

HSP 1 Score: 78.5666 bits (192), Expect = 3.934e-20
Identity = 36/69 (52.17%), Postives = 49/69 (71.01%), Query Frame = 1 
Query:  118 FVLISTQVRLESGPTYVGDEESDPELMTYLNAEKIKALGNNFSHYVSKLKPREIMDLLDKKGFSLISNN 324
            ++LIST++RLE GPT  GDE SDPELM  L+AE +   GNNF  Y+SK  PR +++ L K G+ +IS+ 
Sbjct:    3 YILISTRIRLELGPTQCGDEWSDPELMALLDAELVHQFGNNFKEYLSKHPPRVVLNKLGKIGYKVISST 71
[Close]
BLAST of GTP cyclohydrolase 1 feedback regulatory protein vs. Ensembl Medaka
Match: gchfr (GTP cyclohydrolase I feedback regulator [Source:NCBI gene;Acc:101171044])

HSP 1 Score: 81.2629 bits (199), Expect = 8.570e-21
Identity = 38/67 (56.72%), Postives = 49/67 (73.13%), Query Frame = 1 
Query:  118 FVLISTQVRLESGPTYVGDEESDPELMTYLNAEKIKALGNNFSHYVSKLKPREIMDLLDKKGFSLIS 318
            ++LISTQ+RLE+GPT VGDE SDP +M YL A K   LGNNFS Y  +  PR ++D L+K GF ++S
Sbjct:    3 YILISTQIRLETGPTNVGDEFSDPAVMNYLGARKTTVLGNNFSEYHVEEPPRLVLDKLEKIGFRVLS 69
[Close]
BLAST of GTP cyclohydrolase 1 feedback regulatory protein vs. Ensembl Medaka
Match: gchfr (GTP cyclohydrolase I feedback regulator [Source:NCBI gene;Acc:101171044])

HSP 1 Score: 67.3958 bits (163), Expect = 2.939e-15
Identity = 36/79 (45.57%), Postives = 46/79 (58.23%), Query Frame = 1 
Query:   82 FQLIFSIDFICMFVLISTQVRLESGPTYVGDEESDPELMTYLNAEKIKALGNNFSHYVSKLKPREIMDLLDKKGFSLIS 318
            FQ +F   F C    +      E+GPT VGDE SDP +M YL A K   LGNNFS Y  +  PR ++D L+K GF ++S
Sbjct:    9 FQHLFL--FCCK---VCNHCWQETGPTNVGDEFSDPAVMNYLGARKTTVLGNNFSEYHVEEPPRLVLDKLEKIGFRVLS 82
[Close]
BLAST of GTP cyclohydrolase 1 feedback regulatory protein vs. Planmine SMEST
Match: SMESG000042387.1 (SMESG000042387.1)

HSP 1 Score: 150.984 bits (380), Expect = 1.832e-48
Identity = 76/85 (89.41%), Postives = 76/85 (89.41%), Query Frame = 1 
Query:  115 MFVLISTQVRLESGPTYVGDEESDPELMTYLNAEKIKALGNNFSHYVSKLKPREIMDLLDKKGFSLISNNRSRTNF*FAVLHRKD 369
            MFVLISTQVRLESGPTYVGDEESDPELMTYLNAEKIKALGNNFSHYVSKLKPREIMDLLDKKGFSLIS       F  AVLHRKD
Sbjct:    1 MFVLISTQVRLESGPTYVGDEESDPELMTYLNAEKIKALGNNFSHYVSKLKPREIMDLLDKKGFSLISTTGVGQTF-VAVLHRKD 84
The following BLAST results are available for this feature:
BLAST of GTP cyclohydrolase 1 feedback regulatory protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
GCHFR2.850e-2155.22GTP cyclohydrolase I feedback regulator [Source:HG... [more]
GCHFR1.155e-1466.67GTP cyclohydrolase I feedback regulator [Source:HG... [more]
GCHFR1.207e-1252.94GTP cyclohydrolase I feedback regulator [Source:HG... [more]
GCHFR1.207e-1252.94GTP cyclohydrolase I feedback regulator [Source:HG... [more]
GCHFR2.043e-1143.28GTP cyclohydrolase I feedback regulator [Source:HG... [more]
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BLAST of GTP cyclohydrolase 1 feedback regulatory protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
gfrp-13.681e-2149.25GTP cyclohydrolase I Feedback Regulatory Protein h... [more]
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BLAST of GTP cyclohydrolase 1 feedback regulatory protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GTP cyclohydrolase 1 feedback regulatory protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
gchfr3.314e-2258.21GTP cyclohydrolase I feedback regulator [Source:NC... [more]
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BLAST of GTP cyclohydrolase 1 feedback regulatory protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
eci25.210e-1852.24enoyl-CoA delta isomerase 2 [Source:Xenbase;Acc:XB... [more]
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BLAST of GTP cyclohydrolase 1 feedback regulatory protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Gchfr9.305e-2055.22GTP cyclohydrolase I feedback regulator [Source:MG... [more]
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BLAST of GTP cyclohydrolase 1 feedback regulatory protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q6PBT6|GFRP_DANRE2.502e-2158.21GTP cyclohydrolase 1 feedback regulatory protein O... [more]
sp|P30047|GFRP_HUMAN1.369e-2055.22GTP cyclohydrolase 1 feedback regulatory protein O... [more]
sp|Q32L41|GFRP_BOVIN4.278e-2055.22GTP cyclohydrolase 1 feedback regulatory protein O... [more]
sp|P99025|GFRP_MOUSE6.508e-1955.22GTP cyclohydrolase 1 feedback regulatory protein O... [more]
sp|P70552|GFRP_RAT1.375e-1853.73GTP cyclohydrolase 1 feedback regulatory protein O... [more]
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BLAST of GTP cyclohydrolase 1 feedback regulatory protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A433TTT08.910e-2158.21Uncharacterized protein OS=Elysia chlorotica OX=18... [more]
A0A401P3Z43.298e-2064.18Uncharacterized protein OS=Scyliorhinus torazame O... [more]
Q4RLP34.961e-1959.70Chromosome 10 SCAF15019, whole genome shotgun sequ... [more]
H3DJA15.042e-1959.70GTP cyclohydrolase I feedback regulator OS=Tetraod... [more]
M1ERP89.115e-1958.21GTP cyclohydrolase I feedback regulator (Fragment)... [more]
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BLAST of GTP cyclohydrolase 1 feedback regulatory protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
gchfr1.003e-1049.02GTP cyclohydrolase I feedback regulator [Source:NC... [more]
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BLAST of GTP cyclohydrolase 1 feedback regulatory protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GTP cyclohydrolase 1 feedback regulatory protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GTP cyclohydrolase 1 feedback regulatory protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO320673.934e-2052.17Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of GTP cyclohydrolase 1 feedback regulatory protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2
Match NameE-valueIdentityDescription
gchfr8.570e-2156.72GTP cyclohydrolase I feedback regulator [Source:NC... [more]
gchfr2.939e-1545.57GTP cyclohydrolase I feedback regulator [Source:NC... [more]
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BLAST of GTP cyclohydrolase 1 feedback regulatory protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000042387.11.832e-4889.41SMESG000042387.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30028908 ID=SMED30028908|Name=GTP cyclohydrolase 1 feedback regulatory protein|organism=Schmidtea mediterranea sexual|type=transcript|length=439bp
CGAGAGTCCTTACAATTGTACAAAAAATTCTAATTAATTCGTCACAGATA
TACAATTCATTAAATAGACCTTTCAACAATATTTCAGTTAATCTTTTCCA
TAGATTTTATATGCATGTTTGTTCTAATTAGTACGCAAGTGAGATTAGAA
TCTGGCCCAACATATGTAGGAGACGAGGAAAGCGACCCGGAATTGATGAC
ATATCTAAATGCAGAAAAAATTAAAGCTTTAGGAAATAATTTTTCACATT
ATGTTTCAAAACTAAAGCCCCGGGAAATTATGGATCTCCTGGACAAAAAA
GGATTTTCTCTTATTTCTAACAACAGGAGTAGGACAAACTTTTAGTTCGC
AGTGTTACATAGAAAAGATTGAACAATATTAAAATGGTGAACAATTTGTT
GTCATAAAAATTTATTTAAAAATTTATATAATATAAAAG
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000020protonephridia
PLANA:0000070intestinal phagocyte
PLANA:0000418head
Vocabulary: INTERPRO
TermDefinition
IPR036717GFRP_sf
IPR009112GTP_CycHdrlase_I_reg
Vocabulary: biological process
TermDefinition
GO:0009890negative regulation of biosynthetic process
Vocabulary: molecular function
TermDefinition
GO:0016787hydrolase activity
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR009112GTP cyclohydrolase I, feedback regulatory proteinPFAMPF06399GFRPcoord: 2..75
e-value: 7.2E-27
score: 93.1
IPR009112GTP cyclohydrolase I, feedback regulatory proteinPANTHERPTHR16852FAMILY NOT NAMEDcoord: 2..76
IPR036717GFRP superfamilyGENE3DG3DSA:3.30.1410.10coord: 1..76
e-value: 2.9E-25
score: 89.8
IPR036717GFRP superfamilySUPERFAMILYSSF69761GTP cyclohydrolase I feedback regulatory protein, GFRPcoord: 2..75
NoneNo IPR availablePRODOMPD028559coord: 8..76
e-value: 6.0E-18
score: 216.0