DNA-directed RNA polymerase

Overview
NameDNA-directed RNA polymerase
Smed IDSMED30028902
Length (bp)3505
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of DNA-directed RNA polymerase (SMED30028902) t-SNE clustered cells

Violin plots show distribution of expression levels for DNA-directed RNA polymerase (SMED30028902) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of DNA-directed RNA polymerase (SMED30028902) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for DNA-directed RNA polymerase (SMED30028902) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30028902

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 7

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30028902h1SMcG0018621 SmedASXL_013036SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015
FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30028902h1SMcG0018621 SmedASXL_013036SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015
FACS sorted cell population asexual adult RNA-sequencing evidence
gutSMED30028902h1SMcG0018621 dd_Smed_v4_5515_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30028902h1SMcG0018621 dd_Smed_v4_5515_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30028902h1SMcG0018621 dd_Smed_v4_5515_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30028902h1SMcG0018621 Contig40691uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30028902h1SMcG0018621 Contig40691newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of DNA-directed RNA polymerase vs. Ensembl Human
Match: POLRMT (RNA polymerase mitochondrial [Source:HGNC Symbol;Acc:HGNC:9200])

HSP 1 Score: 507.679 bits (1306), Expect = 4.715e-159
Identity = 336/1014 (33.14%), Postives = 534/1014 (52.66%), Query Frame = 1
Query:  583 TYHSVDKKSIIVDSVFYEPIMKLLAKNGKIDAIITLLNEMDEFHILPNISIIFRTIEAASKNELLNTEFLHRMMGYVKCHNISLEKAILKAPITKENFSHILKALQMIEPNFNIICNKPELKNKSALKETC-----------IFSSIDELEKFFEVQLNNEILTSVNVDPIHNVTVNPEILK----KVILIEDTWRSSLTEAYEKKITLLE---YSDRSKLYPYLSVLPTKSYVDAMIQVVFQLSTDSELQFNRIQHVHLHIGNIISNMFRHRCLKKQKYFAQLKSICLSYYGKL------SENPQNIEMDKLWESSIMENIDSGSIVHDTIDWSKKIKASIGKILYEIMFENMNFTFY---NGITAVTTPVIYEKLE-EDAGKVFITLKIQSKLRTIFKAINKALPGFSQLDMPMTHPPVPFHKNHESGFFYLTNYATRIIRKSTIFDIFDDDLEQ--PDDISKVYNALNVLSLCSWKIENANLDLVMQVFNDSGNMKLKIPTSANKFNGPEKPAVDLP------IKDKLNSWKSYFQFVKEQKEMYSLWSTLSYKIMIAQKLKNKPFWYPYSMDFRGRVYPCSPHLSHIGDDVSRGLLRFSLGKKLGPKGLDWIKIHLINLCGTHKRKSNSDRLKAANEVISEVIDSAANPFDGRCWWQLSEKPWQTLATCREIHKILKFVKSNGKPDDFHSTLPIHQDGSCNGLQHYXXXXXXXXXXXXVNLIQSETPQDVYSDVLEHLEVKRKEDAKNGVEEAILLEGLLSRKVIKXXXXXXXXXXXXXXXXLQILRELKNLEKLPPQNIKSCAAYLSKLTLGSIGEIFASATNIQNWFIELSSNISRLRKANISWKTPMNFIVKQPYFAD-----------------DSDI-LKSDCLKNKNAFPPNFIHSLDSTHMMLTALSCWEKNITFVSVHDCFWTHACDVDEMNKICREEFVKLHSQPILENLSDYLLEKYQFQINPNENDNGRKIAFNKLLKNIPKKGTFDIREVLDSTYFFS 3462
             +H   +K  ++    Y  +M   A+ G    ++ +L  + +  + P++      ++   + +  +   + R +  +    + L+       +++E+ + +LKA+  ++P F++    P   N S L               +   +  L+  FE QL+ E+ + V V  +   T+  + +K     +  + D W  +L  A  +    LE   Y  R  LYP+L +L  +  V  ++QV+  L    E  F  +         + +  F    +++Q+   Q++++   Y   L      +E P+     + WE      + +   + +   W   ++  +GK+L E++ +             ++   PV+Y      +  ++ I     + ++ + KA    L  F  +D+PM  PP+P+   H SG F L+   T+++R         + LE   P  +    +AL  L  C+W++    LDLV+Q+F   G  +L +P   ++   P+ P   LP       K +L    ++ Q V   +EM+SL +   Y++ +AQ L+++ FW P++MDFRGR YPC PH +H+G DV+R LL F+ G+ LGP GLDW+KIHL+NL G  KR+    RL  A EV+ +++DSA  P  GR WW  +E+PWQTLA C E+   ++       P  + S LP+HQDGSCNGLQHYAALG+D+ GAA+VNL  S+ PQDVYS V   +EV R++DA+ G+  A +LEG ++RKV+KQTVMT VYGVT+YGG LQI + L+ L   P + +   + YL +    S+ E+F+    IQ+W  E +  IS +  + + W TP+   V QPY  D                 + DI  K +  K KN FPPNFIHSLDS+HMMLTAL C+ K +TFVSVHDC+WTHA DV  MN++CRE+FV+LHS+PIL++LS +L+++  F   P +     ++   + L+ +PK G FD+ +V  STYFFS
Sbjct:  248 VHHGQRQKRKLLTLDMYNAVMLGWARQGAFKELVYVLFMVKDAGLTPDLLSYAAALQCMGRQDQ-DAGTIERCLEQMSQEGLKLQALFTAVLLSEEDRATVLKAVHKVKPTFSLPPQLPPPVNTSKLLRDVYAKDGRVSYPKLHLPLKTLQCLFEKQLHMELASRVCVVSVEKPTLPSKEVKHARKTLKTLRDQWEKALCRALRETKNRLEREVYEGRFSLYPFLCLLDEREVVRMLLQVLQALPAQGE-SFTTLAR------ELSARTFSRHVVQRQRVSGQVQALQNHYRKYLCLLASDAEVPEPCLPRQYWE-----ELGAPEALREQ-PWPLPVQMELGKLLAEMLVQATQMPCSLDKPHRSSRLVPVLYHVYSFRNVQQIGILKPHPAYVQLLEKAAEPTL-TFEAVDVPMLCPPLPWTSPH-SGAFLLS--PTKLMRTVEGATQHQELLETCPPTALHGALDALTQLGNCAWRVNGRVLDLVLQLFQAKGCPQLGVPAPPSE--APQPPEAHLPHSAAPARKAELRRELAHCQKVA--REMHSLRAEALYRLSLAQHLRDRVFWLPHNMDFRGRTYPCPPHFNHLGSDVARALLEFAQGRPLGPHGLDWLKIHLVNLTGLKKREPLRKRLAFAEEVMDDILDSADQPLTGRKWWMGAEEPWQTLACCMEVANAVR----ASDPAAYVSHLPVHQDGSCNGLQHYAALGRDSVGAASVNLEPSDVPQDVYSGVAAQVEVFRRQDAQRGMRVAQVLEGFITRKVVKQTVMTVVYGVTRYGGRLQIEKRLRELSDFPQEFVWEASHYLVRQVFKSLQEMFSGTRAIQHWLTESARLISHM-GSVVEWVTPLGVPVIQPYRLDSKVKQIGGGIQSITYTHNGDISRKPNTRKQKNGFPPNFIHSLDSSHMMLTALHCYRKGLTFVSVHDCYWTHAADVSVMNQVCREQFVRLHSEPILQDLSRFLVKR--FCSEPQKILEASQL--KETLQAVPKPGAFDLEQVKRSTYFFS 1230          
BLAST of DNA-directed RNA polymerase vs. Ensembl Human
Match: POLRMT (RNA polymerase mitochondrial [Source:HGNC Symbol;Acc:HGNC:9200])

HSP 1 Score: 129.798 bits (325), Expect = 1.755e-34
Identity = 53/78 (67.95%), Postives = 69/78 (88.46%), Query Frame = 1
Query: 3100 KNKNAFPPNFIHSLDSTHMMLTALSCWEKNITFVSVHDCFWTHACDVDEMNKICREEFVKLHSQPILENLSDYLLEKY 3333
            K KN FPPNFIHSLDS+HMMLTAL C+ K +TFVSVHDC+WTHA DV  MN++CRE+FV+LHS+PIL++LS +L++++
Sbjct:   31 KQKNGFPPNFIHSLDSSHMMLTALHCYRKGLTFVSVHDCYWTHAADVSVMNQVCREQFVRLHSEPILQDLSRFLVKRF 108          
BLAST of DNA-directed RNA polymerase vs. Ensembl Celegans
Match: rpom-1 (DNA-directed RNA polymerase [Source:UniProtKB/TrEMBL;Acc:Q8WQA3])

HSP 1 Score: 418.313 bits (1074), Expect = 4.640e-127
Identity = 284/781 (36.36%), Postives = 431/781 (55.19%), Query Frame = 1
Query: 1270 SELQFNRIQHVHLHIGNIISNMFRHRCLKKQKYFAQLKSICLSYYGKLSENPQNIEMDKLWESSIMENIDSGSIVHDTIDWSKKIKASIGKILYEIMFENMNFTFYNGITAVTTPVIYE----KLEEDA-----GKVFIT--LKIQSKLRTIFKAINKALPGFSQLDMPMTHPPVPFHKNHESGFFYLTNYATRIIRKSTIFDIFDDDLEQPDDISK------VYNALNVLSLCSWKIENANLDLVMQVFNDSGNM-------KLKIPTSANKFNGPE----------KPAVDLPIKDKLNSW-KSYFQFVKEQKEMYSLWSTLSYKIMIAQKLKNKPFWYPYSMDFRGRVYPCSPHLSHIGDDVSRGLLRFSLGKKLGPKGLDWIKIHLINLCGTHKRKSNSDRLKAANEVISEVIDSAANPFDGRCWWQLSEKPWQTLATCREIHKILKFVKSNGKPDDFHSTLPIHQDGSCNGLQHYXXXXXXXXXXXXVNLIQSETPQDVYSDVLEHLEVKRKEDAKNGVEEAILLEGL-------LSRKVIKXXXXXXXXXXXXXXXXLQILRELKNLEKLPPQNIKSCAAYLSKLTLGSIGEIFASATNIQNWFIELSSNISRLRKANISWKTPMNFIVKQPYFA----DDSDILKSDCLKNKNAFPPNFIHSLDSTHMMLTALSCWEKNITFVSVHDCFWTHACDVDEMNKICREEFVKLHSQPILENLSDYLLEKY----QFQINPNENDNGRKIAFNKLLKNIPKKGTFDIREVLDSTYFFS 3462
            S+ QF+ ++ +   +GN       +    + + + ++ +  + Y+     + QN   +  W  S      +     +  +   + +  +  +L  ++F++  F + +  + VT    +      +EE++     G++ ++  L I SKL  +F         FS  ++PM  PP P+      G  Y T+    IIRK   F     + E    I+       V++ALN L    W I    LD++  VF  SGN        KL+IP   + F+ P+          K  VD+   +K  S+ K   + +K + E  SLW  + Y++++A   + +  ++P++MDFRGRVYP SP+LSH+GDDV+R +L+F+  +KLG KG DW+K+H INL GT KR S +DR+  A  +I  ++DSA +P +G+ WW  S++PWQTLA C EI    ++         F S LPIHQDGSCNGLQHYAALG+D +G   VNL QS+TP DVYSDV + +E KR++D ++  E+  +   L       + RKVIKQTVMTTVYGVT YG  LQI R+LK ++ +P ++    A YL++ T  S+ + F S+  +++WF  ++   S L K  + W TP+   V QPY          IL    +K  +AFPPNF+HSLDSTHMMLT+L+C ++ ITF +VHDCFWTHA  VD+MN+ICR++FV LHSQPI+E  S++  + Y      +I P ++       ++++     + G  DI +V DS YFFS
Sbjct:  375 SQFQFDVLEPILKELGNAFKKEMGY---DENEIWKRVFATYIDYFSDSEISRQNTHRE-WWNKSCKTQGITSDFQFEMGNMDSETRNEVCNVLINVIFDSCLFPYESKDSKVTWQRAFSYRSISIEEESKVSTDGRLSLSRMLSINSKLMGLFDKNPFNFIVFSTSNLPMIVPPRPWIDRGTGGPLYTTS-QHEIIRKMMEFRAVRLNEEMRARITSQAQARPVFDALNQLGSTPWVINEPMLDVLKDVFGRSGNSESRGLLEKLEIPMRHDTFDIPDFGREFGIGIKKEDVDV---EKFRSYAKKKAEAIKMRNESNSLWCWMQYRVVLADHFRGQTLFFPHNMDFRGRVYPLSPYLSHMGDDVNRCILKFAKSQKLGEKGFDWLKLHCINLTGTMKRSSVADRMVEAERLIPLMLDSARDPLNGQKWWMSSDEPWQTLAACVEIENATRYGSDVAL---FPSQLPIHQDGSCNGLQHYAALGRDNEGGVQVNLTQSDTPNDVYSDVAQRVEQKRQQDEQSNGEDCDVARKLREALPQNVPRKVIKQTVMTTVYGVTMYGAVLQIKRQLKAMD-IPGEDAAIFARYLARKTFASLNDAFTSSMALKDWFRLIAKGSSDLMKT-VEWITPLGLPVVQPYCKLVERKGKLILAPVPMKQVDAFPPNFVHSLDSTHMMLTSLNCAQRGITFAAVHDCFWTHANSVDQMNEICRQQFVSLHSQPIVEQCSEWFKKTYLTPKMAKILPEKDFQ----KYSEIFTANIEHGDLDIEKVKDSVYFFS 1138          
BLAST of DNA-directed RNA polymerase vs. Ensembl Celegans
Match: rpom-1 (DNA-directed RNA polymerase [Source:UniProtKB/TrEMBL;Acc:Q8WQA3])

HSP 1 Score: 418.313 bits (1074), Expect = 4.640e-127
Identity = 284/781 (36.36%), Postives = 431/781 (55.19%), Query Frame = 1
Query: 1270 SELQFNRIQHVHLHIGNIISNMFRHRCLKKQKYFAQLKSICLSYYGKLSENPQNIEMDKLWESSIMENIDSGSIVHDTIDWSKKIKASIGKILYEIMFENMNFTFYNGITAVTTPVIYE----KLEEDA-----GKVFIT--LKIQSKLRTIFKAINKALPGFSQLDMPMTHPPVPFHKNHESGFFYLTNYATRIIRKSTIFDIFDDDLEQPDDISK------VYNALNVLSLCSWKIENANLDLVMQVFNDSGNM-------KLKIPTSANKFNGPE----------KPAVDLPIKDKLNSW-KSYFQFVKEQKEMYSLWSTLSYKIMIAQKLKNKPFWYPYSMDFRGRVYPCSPHLSHIGDDVSRGLLRFSLGKKLGPKGLDWIKIHLINLCGTHKRKSNSDRLKAANEVISEVIDSAANPFDGRCWWQLSEKPWQTLATCREIHKILKFVKSNGKPDDFHSTLPIHQDGSCNGLQHYXXXXXXXXXXXXVNLIQSETPQDVYSDVLEHLEVKRKEDAKNGVEEAILLEGL-------LSRKVIKXXXXXXXXXXXXXXXXLQILRELKNLEKLPPQNIKSCAAYLSKLTLGSIGEIFASATNIQNWFIELSSNISRLRKANISWKTPMNFIVKQPYFA----DDSDILKSDCLKNKNAFPPNFIHSLDSTHMMLTALSCWEKNITFVSVHDCFWTHACDVDEMNKICREEFVKLHSQPILENLSDYLLEKY----QFQINPNENDNGRKIAFNKLLKNIPKKGTFDIREVLDSTYFFS 3462
            S+ QF+ ++ +   +GN       +    + + + ++ +  + Y+     + QN   +  W  S      +     +  +   + +  +  +L  ++F++  F + +  + VT    +      +EE++     G++ ++  L I SKL  +F         FS  ++PM  PP P+      G  Y T+    IIRK   F     + E    I+       V++ALN L    W I    LD++  VF  SGN        KL+IP   + F+ P+          K  VD+   +K  S+ K   + +K + E  SLW  + Y++++A   + +  ++P++MDFRGRVYP SP+LSH+GDDV+R +L+F+  +KLG KG DW+K+H INL GT KR S +DR+  A  +I  ++DSA +P +G+ WW  S++PWQTLA C EI    ++         F S LPIHQDGSCNGLQHYAALG+D +G   VNL QS+TP DVYSDV + +E KR++D ++  E+  +   L       + RKVIKQTVMTTVYGVT YG  LQI R+LK ++ +P ++    A YL++ T  S+ + F S+  +++WF  ++   S L K  + W TP+   V QPY          IL    +K  +AFPPNF+HSLDSTHMMLT+L+C ++ ITF +VHDCFWTHA  VD+MN+ICR++FV LHSQPI+E  S++  + Y      +I P ++       ++++     + G  DI +V DS YFFS
Sbjct:  375 SQFQFDVLEPILKELGNAFKKEMGY---DENEIWKRVFATYIDYFSDSEISRQNTHRE-WWNKSCKTQGITSDFQFEMGNMDSETRNEVCNVLINVIFDSCLFPYESKDSKVTWQRAFSYRSISIEEESKVSTDGRLSLSRMLSINSKLMGLFDKNPFNFIVFSTSNLPMIVPPRPWIDRGTGGPLYTTS-QHEIIRKMMEFRAVRLNEEMRARITSQAQARPVFDALNQLGSTPWVINEPMLDVLKDVFGRSGNSESRGLLEKLEIPMRHDTFDIPDFGREFGIGIKKEDVDV---EKFRSYAKKKAEAIKMRNESNSLWCWMQYRVVLADHFRGQTLFFPHNMDFRGRVYPLSPYLSHMGDDVNRCILKFAKSQKLGEKGFDWLKLHCINLTGTMKRSSVADRMVEAERLIPLMLDSARDPLNGQKWWMSSDEPWQTLAACVEIENATRYGSDVAL---FPSQLPIHQDGSCNGLQHYAALGRDNEGGVQVNLTQSDTPNDVYSDVAQRVEQKRQQDEQSNGEDCDVARKLREALPQNVPRKVIKQTVMTTVYGVTMYGAVLQIKRQLKAMD-IPGEDAAIFARYLARKTFASLNDAFTSSMALKDWFRLIAKGSSDLMKT-VEWITPLGLPVVQPYCKLVERKGKLILAPVPMKQVDAFPPNFVHSLDSTHMMLTSLNCAQRGITFAAVHDCFWTHANSVDQMNEICRQQFVSLHSQPIVEQCSEWFKKTYLTPKMAKILPEKDFQ----KYSEIFTANIEHGDLDIEKVKDSVYFFS 1138          
BLAST of DNA-directed RNA polymerase vs. Ensembl Celegans
Match: rpom-1 (DNA-directed RNA polymerase [Source:UniProtKB/TrEMBL;Acc:Q8WQA3])

HSP 1 Score: 407.912 bits (1047), Expect = 4.944e-123
Identity = 285/799 (35.67%), Postives = 434/799 (54.32%), Query Frame = 1
Query: 1270 SELQFNRIQHVHLHIGNIISNMFRHRCLKKQKYFAQLKSICLSYYGKLSENPQNIEMDKLWESSIMENIDSGSIVHDTIDWSKKIKASIGKILYEIMFENMNFTFYNGITAVTTPVIYE----KLEEDA-----GKVFIT--LKIQSKLRTIFKAINKALPGFSQLDMPMTHPPVPFHKNHESGFFYLTNYATRIIRKSTIFDIFDDDLEQPDDISK------VYNALNVLSLCSWKIENANLDLVMQVFNDSGNM-------KLKIPTSANKFNGPE----------KPAVDLPIKDKLNSW-KSYFQFVKEQKEMYSLWSTLSYKIMIAQKLKNKPFWYPYSMDFRGRVYPCSPHLSHIGDDVSRGLLRFSLGKKLGPKGLDWIKIHLINLCGTHKRKSNSDRLKAANEVISEVIDSAANPFDGRCWWQLSEKPWQTLATCREIHKILKFVKSNGKPDDFHSTLPIHQDGSCNGLQHYXXXXXXXXXXXXVNLIQSETPQDVYSDVLEHLEVKRKEDAKNGVEEAILLEGL-------LSRKVIKXXXXXXXXXXXXXXXXLQILRELKNLEKLPPQNI---------KSCAA---------YLSKLTLGSIGEIFASATNIQNWFIELSSNISRLRKANISWKTPMNFIVKQPYFA----DDSDILKSDCLKNKNAFPPNFIHSLDSTHMMLTALSCWEKNITFVSVHDCFWTHACDVDEMNKICREEFVKLHSQPILENLSDYLLEKY----QFQINPNENDNGRKIAFNKLLKNIPKKGTFDIREVLDSTYFFS 3462
            S+ QF+ ++ +   +GN       +    + + + ++ +  + Y+     + QN   +  W  S      +     +  +   + +  +  +L  ++F++  F + +  + VT    +      +EE++     G++ ++  L I SKL  +F         FS  ++PM  PP P+      G  Y T+    IIRK   F     + E    I+       V++ALN L    W I    LD++  VF  SGN        KL+IP   + F+ P+          K  VD+   +K  S+ K   + +K + E  SLW  + Y++++A   + +  ++P++MDFRGRVYP SP+LSH+GDDV+R +L+F+  +KLG KG DW+K+H INL GT KR S +DR+  A  +I  ++DSA +P +G+ WW  S++PWQTLA C EI    ++         F S LPIHQDGSCNGLQHYAALG+D +G   VNL QS+TP DVYSDV + +E KR++D ++  E+  +   L       + RKVIKQTVMTTVYGVT YG  LQI R+LK ++ +P ++          K+ A+         YL++ T  S+ + F S+  +++WF  ++   S L K  + W TP+   V QPY          IL    +K  +AFPPNF+HSLDSTHMMLT+L+C ++ ITF +VHDCFWTHA  VD+MN+ICR++FV LHSQPI+E  S++  + Y      +I P ++       ++++     + G  DI +V DS YFFS
Sbjct:  375 SQFQFDVLEPILKELGNAFKKEMGY---DENEIWKRVFATYIDYFSDSEISRQNTHRE-WWNKSCKTQGITSDFQFEMGNMDSETRNEVCNVLINVIFDSCLFPYESKDSKVTWQRAFSYRSISIEEESKVSTDGRLSLSRMLSINSKLMGLFDKNPFNFIVFSTSNLPMIVPPRPWIDRGTGGPLYTTS-QHEIIRKMMEFRAVRLNEEMRARITSQAQARPVFDALNQLGSTPWVINEPMLDVLKDVFGRSGNSESRGLLEKLEIPMRHDTFDIPDFGREFGIGIKKEDVDV---EKFRSYAKKKAEAIKMRNESNSLWCWMQYRVVLADHFRGQTLFFPHNMDFRGRVYPLSPYLSHMGDDVNRCILKFAKSQKLGEKGFDWLKLHCINLTGTMKRSSVADRMVEAERLIPLMLDSARDPLNGQKWWMSSDEPWQTLAACVEIENATRYGSDVAL---FPSQLPIHQDGSCNGLQHYAALGRDNEGGVQVNLTQSDTPNDVYSDVAQRVEQKRQQDEQSNGEDCDVARKLREALPQNVPRKVIKQTVMTTVYGVTMYGAVLQIKRQLKAMD-IPGEDAAIFARYLARKTFASLNDAAIFARYLARKTFASLNDAFTSSMALKDWFRLIAKGSSDLMKT-VEWITPLGLPVVQPYCKLVERKGKLILAPVPMKQVDAFPPNFVHSLDSTHMMLTSLNCAQRGITFAAVHDCFWTHANSVDQMNEICRQQFVSLHSQPIVEQCSEWFKKTYLTPKMAKILPEKDFQ----KYSEIFTANIEHGDLDIEKVKDSVYFFS 1156          
BLAST of DNA-directed RNA polymerase vs. Ensembl Fly
Match: mtRNApol (gene:FBgn0261938 transcript:FBtr0332273)

HSP 1 Score: 503.827 bits (1296), Expect = 1.323e-156
Identity = 346/1064 (32.52%), Postives = 528/1064 (49.62%), Query Frame = 1
Query:  502 TYMDVCIQKGDYEKALSLFNSVRNE--NETYHSVDKKSIIVDSVFYEPIMKLLAKNGKIDAIITLLNEMDEFHILPN---ISIIFRTIEAASKNELLNTEFLHRMMGYVKCHNISLEKAILKAPITKENFSHILKALQMIEPNFNIICNKPEL-------------------KNKSALKETCIFSS-----IDELEKFFEVQLNNEILTSVNVDPIHNVT--VNPEILKKVIL-IEDTWRSSLTEAYEKKITLLEYSDRSK------LYPYLSVLPTKSYVDAMIQVVFQLSTDSELQFNRIQHVHLHIGNIISNMFRHRCLKKQKYFAQLKSICLSYYGKLSENPQNIEMDKLWESSIMENIDSG-SIVHDTIDWSKKIKASIGKILYEIMFENMNFTFY-----NGITAVTTP-----VIYEKLEEDAGKVFITLKIQSKLRTIFKAINKALPGFSQLDMPMTHPPVPFHKNHESGFFYLTNYATRIIRKSTIFDIFDDDLEQ-PDDISKVYNALNVLSLCSWKIENANLDLVMQVFNDSGNMKLKIPTSANKFN------GPEKPAVDLPIKDKLNSWKSYFQFVKEQKEMYSLWSTLSYKIMIAQKLKNKPFWYPYSMDFRGRVYPCSPHLSHIGDDVSRGLLRFSLGKKLGPKGLDWIKIHLINLCGTHKRKSNSDRLKAANEVISEVIDSAANPFDGRCWWQLSEKPWQTLATCREIHKILKFVKSNGKPDDFHSTLPIHQDGSCNGLQHYXXXXXXXXXXXXVNLIQSETPQDVYSDVLEHLEVKRKEDAKNGVEEAILLEGLLSRKVIKXXXXXXXXXXXXXXXXLQILRELKNLEKLPPQNIKSCAAYLSKLTLGSIGEIFASATNIQNWFIELSSNISRLRKANISWKTPMNFIVKQPY------------FADDSDIL-----KSDCLKNKNAFPPNFIHSLDSTHMMLTALSCWEKNITFVSVHDCFWTHACDVDEMNKICREEFVKLHSQPILENLSDYLLEKYQFQ----INPNENDNGRKIAFNKLLKNIPKKGTFDIREVLDSTYFFS 3462
            TY++VC+      +ALS   + R +    +   + +  I +D   Y  ++   A  G  D    +   ++E  +  N    + IF  +     +E  N +++   +   +    SL + + ++    +     L A++ + P+F  +   P+L                   K +    +  I +S       +LE+    QL  E+  S+ +  I       N E  ++++  +EDTWR  ++ A  + +  L    R K       Y YL  L    + D +I+ +++L+  SE     +  ++  +G  +   ++    K      ++  I  SY              + W+  + E  +SG S+    + W   +   +G+ LY I+  ++    +     +   A   P       Y         V   +K    L  +F+A  +    F    +PM  PP P+   H  G  YL N    I         +D  +   P  +    ++LN L+   W++    LD++++VF + G++KL +P   +           +        +D+   ++      ++Q EMYSLW    Y++ +AQ  ++K FW P++MDFRGRVYP  PHL+H+G D++R +L F   + LG  GL W+K+H INL G  KR S  +RL  A E++ +++DSA NP  GR WW  S++PWQTLA C EI  + +       P  + S  PIHQDGSCNGLQHYAALG+D  GA +VNL  S  PQDVYS V   +E  RK DA+NG+  A  L G + RKVIKQTVMTTVYGVT+YG  LQI R+LK+++  P   +   + YL+  T  S+ E+F S   IQ+WF E +  IS +   N+ W TP+   V QPY            F   +++      + + LK KNAFPPNFIHSLDS+HMMLT+L C  + ITFVSVHDCFWTHA  V E+N++CRE+FV LHSQPILE LS+++   Y F+    IN    ++  K   N++LK +P+KG FD+R VLDS YFFS
Sbjct:  315 TYLNVCVSANMLNRALSTIIAYRGKVRKSSLPHMSEGLITID--LYNILLHGYAGKGSFDRAQEVFKLIEEDGLSFNEQTYAAIFECLGRLEPDEE-NHQYISEYVSKAEKEGFSLNQIMDRSKFVADQRDIALDAIRRLRPDFKPVYVPPQLGYDNELLDHLNEHVIPVGAKKEPGKADYAIMNSKRGYTAAQLEQLAREQLKIELDGSITIKSIEKSKEFANSEKCRELLKELEDTWRKQISAAIVRDLNTLRAQVRFKPHGFMNYYTYLKTLDASHFADILIKELYKLAEGSETFSPTVGQLYKELGQKVQQRYQIEQKKHNGTLEKIGEIYSSYCELWGSGMSQDNTRQAWQRLVYEQRESGPSMDLLEVPWPSNVLTGVGRFLYNILMRDIKIDAHIMRQKSKSKAAAQPQNLLPAFYTLFRNQGRFVKEEVKPHPVLSRLFRASRQQTLTFDSNLVPMLCPPQPWSTPHNGG--YLLNKTELIRLPHQAIQQWDRIMASNPQHLYPALDSLNQLASVPWRVNTQLLDVIIKVFQNGGDVKLDVPQPPSSLPPLPTLPAKDAEGNATSSEDRAKQFRDKLGHRRKQAEMYSLWCDALYRLSLAQHYRDKVFWLPHNMDFRGRVYPVPPHLNHLGSDLARSMLIFDQAQPLGVDGLSWLKLHCINLTGLKKRDSVRERLLYAEEIMPDILDSADNPLTGRMWWSKSDEPWQTLACCMEIANVHRC----PDPAAYLSRFPIHQDGSCNGLQHYAALGRDEAGARSVNLAPSAIPQDVYSAVAALVEKSRKADAQNGLHVAEALAGFVRRKVIKQTVMTTVYGVTRYGARLQIARQLKDIDDFPKDWVWPASTYLTTKTFESLREMFTSTREIQDWFTECARLISGVCSQNVEWVTPLGLPVVQPYNRQEMKHSPRSGFKVSANMPMDLYERPNILKQKNAFPPNFIHSLDSSHMMLTSLHCERQGITFVSVHDCFWTHANTVPELNRMCREQFVALHSQPILEQLSEFMRHTYSFKDSDFINDGSVEDLSKRQLNRILKQMPQKGDFDLRNVLDSVYFFS 1369          
BLAST of DNA-directed RNA polymerase vs. Ensembl Fly
Match: mtRNApol (gene:FBgn0261938 transcript:FBtr0077989)

HSP 1 Score: 503.827 bits (1296), Expect = 1.323e-156
Identity = 346/1064 (32.52%), Postives = 528/1064 (49.62%), Query Frame = 1
Query:  502 TYMDVCIQKGDYEKALSLFNSVRNE--NETYHSVDKKSIIVDSVFYEPIMKLLAKNGKIDAIITLLNEMDEFHILPN---ISIIFRTIEAASKNELLNTEFLHRMMGYVKCHNISLEKAILKAPITKENFSHILKALQMIEPNFNIICNKPEL-------------------KNKSALKETCIFSS-----IDELEKFFEVQLNNEILTSVNVDPIHNVT--VNPEILKKVIL-IEDTWRSSLTEAYEKKITLLEYSDRSK------LYPYLSVLPTKSYVDAMIQVVFQLSTDSELQFNRIQHVHLHIGNIISNMFRHRCLKKQKYFAQLKSICLSYYGKLSENPQNIEMDKLWESSIMENIDSG-SIVHDTIDWSKKIKASIGKILYEIMFENMNFTFY-----NGITAVTTP-----VIYEKLEEDAGKVFITLKIQSKLRTIFKAINKALPGFSQLDMPMTHPPVPFHKNHESGFFYLTNYATRIIRKSTIFDIFDDDLEQ-PDDISKVYNALNVLSLCSWKIENANLDLVMQVFNDSGNMKLKIPTSANKFN------GPEKPAVDLPIKDKLNSWKSYFQFVKEQKEMYSLWSTLSYKIMIAQKLKNKPFWYPYSMDFRGRVYPCSPHLSHIGDDVSRGLLRFSLGKKLGPKGLDWIKIHLINLCGTHKRKSNSDRLKAANEVISEVIDSAANPFDGRCWWQLSEKPWQTLATCREIHKILKFVKSNGKPDDFHSTLPIHQDGSCNGLQHYXXXXXXXXXXXXVNLIQSETPQDVYSDVLEHLEVKRKEDAKNGVEEAILLEGLLSRKVIKXXXXXXXXXXXXXXXXLQILRELKNLEKLPPQNIKSCAAYLSKLTLGSIGEIFASATNIQNWFIELSSNISRLRKANISWKTPMNFIVKQPY------------FADDSDIL-----KSDCLKNKNAFPPNFIHSLDSTHMMLTALSCWEKNITFVSVHDCFWTHACDVDEMNKICREEFVKLHSQPILENLSDYLLEKYQFQ----INPNENDNGRKIAFNKLLKNIPKKGTFDIREVLDSTYFFS 3462
            TY++VC+      +ALS   + R +    +   + +  I +D   Y  ++   A  G  D    +   ++E  +  N    + IF  +     +E  N +++   +   +    SL + + ++    +     L A++ + P+F  +   P+L                   K +    +  I +S       +LE+    QL  E+  S+ +  I       N E  ++++  +EDTWR  ++ A  + +  L    R K       Y YL  L    + D +I+ +++L+  SE     +  ++  +G  +   ++    K      ++  I  SY              + W+  + E  +SG S+    + W   +   +G+ LY I+  ++    +     +   A   P       Y         V   +K    L  +F+A  +    F    +PM  PP P+   H  G  YL N    I         +D  +   P  +    ++LN L+   W++    LD++++VF + G++KL +P   +           +        +D+   ++      ++Q EMYSLW    Y++ +AQ  ++K FW P++MDFRGRVYP  PHL+H+G D++R +L F   + LG  GL W+K+H INL G  KR S  +RL  A E++ +++DSA NP  GR WW  S++PWQTLA C EI  + +       P  + S  PIHQDGSCNGLQHYAALG+D  GA +VNL  S  PQDVYS V   +E  RK DA+NG+  A  L G + RKVIKQTVMTTVYGVT+YG  LQI R+LK+++  P   +   + YL+  T  S+ E+F S   IQ+WF E +  IS +   N+ W TP+   V QPY            F   +++      + + LK KNAFPPNFIHSLDS+HMMLT+L C  + ITFVSVHDCFWTHA  V E+N++CRE+FV LHSQPILE LS+++   Y F+    IN    ++  K   N++LK +P+KG FD+R VLDS YFFS
Sbjct:  315 TYLNVCVSANMLNRALSTIIAYRGKVRKSSLPHMSEGLITID--LYNILLHGYAGKGSFDRAQEVFKLIEEDGLSFNEQTYAAIFECLGRLEPDEE-NHQYISEYVSKAEKEGFSLNQIMDRSKFVADQRDIALDAIRRLRPDFKPVYVPPQLGYDNELLDHLNEHVIPVGAKKEPGKADYAIMNSKRGYTAAQLEQLAREQLKIELDGSITIKSIEKSKEFANSEKCRELLKELEDTWRKQISAAIVRDLNTLRAQVRFKPHGFMNYYTYLKTLDASHFADILIKELYKLAEGSETFSPTVGQLYKELGQKVQQRYQIEQKKHNGTLEKIGEIYSSYCELWGSGMSQDNTRQAWQRLVYEQRESGPSMDLLEVPWPSNVLTGVGRFLYNILMRDIKIDAHIMRQKSKSKAAAQPQNLLPAFYTLFRNQGRFVKEEVKPHPVLSRLFRASRQQTLTFDSNLVPMLCPPQPWSTPHNGG--YLLNKTELIRLPHQAIQQWDRIMASNPQHLYPALDSLNQLASVPWRVNTQLLDVIIKVFQNGGDVKLDVPQPPSSLPPLPTLPAKDAEGNATSSEDRAKQFRDKLGHRRKQAEMYSLWCDALYRLSLAQHYRDKVFWLPHNMDFRGRVYPVPPHLNHLGSDLARSMLIFDQAQPLGVDGLSWLKLHCINLTGLKKRDSVRERLLYAEEIMPDILDSADNPLTGRMWWSKSDEPWQTLACCMEIANVHRC----PDPAAYLSRFPIHQDGSCNGLQHYAALGRDEAGARSVNLAPSAIPQDVYSAVAALVEKSRKADAQNGLHVAEALAGFVRRKVIKQTVMTTVYGVTRYGARLQIARQLKDIDDFPKDWVWPASTYLTTKTFESLREMFTSTREIQDWFTECARLISGVCSQNVEWVTPLGLPVVQPYNRQEMKHSPRSGFKVSANMPMDLYERPNILKQKNAFPPNFIHSLDSSHMMLTSLHCERQGITFVSVHDCFWTHANTVPELNRMCREQFVALHSQPILEQLSEFMRHTYSFKDSDFINDGSVEDLSKRQLNRILKQMPQKGDFDLRNVLDSVYFFS 1369          
BLAST of DNA-directed RNA polymerase vs. Ensembl Zebrafish
Match: polrmt (polymerase (RNA) mitochondrial (DNA directed) [Source:ZFIN;Acc:ZDB-GENE-030131-1528])

HSP 1 Score: 525.013 bits (1351), Expect = 3.203e-165
Identity = 358/1041 (34.39%), Postives = 557/1041 (53.51%), Query Frame = 1
Query:  505 YMDVCIQKGDYEKALSLFNSVRNENETYHSVDKKSIIVDSVFYEPIMKLLAKNGKIDAIITLLNEMDEFHILPNISIIFRTIEAASKNELLNTEFLHRMMGYVKCHNISLEKAILKAPITKENFSHILKALQMIEPNFNI-------ICNKPELKNKSALKETCIFSSID----ELEKFFEVQLNNEILTSV---NVDPIHNVTVNPEILKKVILIEDT-WRSSLTEAYEKKITLL----EYSDRSKLYPYLSVLPTKSYVDAMIQVVFQLSTDSELQFNRIQHVHLHIGNIISNMFRHRCLKKQKYFAQLKSICLSYYGKLSENPQNIEM--DKLWES-SIMENIDSGSIVHDTIDWSKKIKASIGKILYEIMFENMNFT---FYNGITAVTTPVIYEKLEEDAGKVFITLKIQSKLRTIFKAINKALPGFSQLDMPMTHPPVPFHKNHESGFFYLTNYATRIIRKSTIFDIFDDDLE--QPDDISKVYNALNVLSLCSWKIENANLDLVMQVFNDSGNMKLKIPTSANKFNGPEKPAVDLPIKDKLNSWKSYF--QFVKEQK---EMYSLWSTLSYKIMIAQKLKNKPFWYPYSMDFRGRVYPCSPHLSHIGDDVSRGLLRFSLGKKLGPKGLDWIKIHLINLCGTHKRKSNSDRLKAANEVISEVIDSAANPFDGRCWWQLSEKPWQTLATCREIHKILKFVKSNGKPD--DFHSTLPIHQDGSCNGLQHYXXXXXXXXXXXXVNLIQSETPQDVYSDVLEHLEVKRKEDAKNGVEEAILLEGLLSRKVIKXXXXXXXXXXXXXXXXLQILRELKNLEKLPPQNIKSCAAYLSKLTLGSIGEIFASATNIQNWFIELSSNISRLRKANISWKTPMNFIVKQPYFADDSDILKS-----------------DCLKNKNAFPPNFIHSLDSTHMMLTALSCWEKNITFVSVHDCFWTHACDVDEMNKICREEFVKLHSQPILENLSDYLLEKYQFQINPNENDNGRKI----AFNKLLKNIPKKGTFDIREVLDSTYFFS 3462
            Y++ C+   D  +A +   S       +  + K+S +  S  Y  +M++ AK G I+ I  L + ++E  + PN++     +E   + +   T  + R +  +    +S++  + +    K+    +L+A+Q++ P+F         +C+ P +K   A +E  I+  +D    EL+  F +QL  E  T+V   +V+ +  VT N   +++++  +   W  +L +A + +  ++    + S  + LYPYL +L  + YVD M+Q + +LS + E     +  +   +GN + N++  R   + +   +L+SI  SY   L+ + +   M   + W +  I  N    S++ D   W   +   +G  L ++M   +          +     P++Y      +      +K    L  I +   +    F    MPM  PPVP+  + +SG + LT    +++R S         LE  Q +D+  V ++LN +  C WKI    LD+++ +FND G+ KL IP   ++   PE P  +          K++   + +K +K   EM+SL     YK+ IA  +++K FW+P++MDFRGR YP  P+ +H+G DV+R LL F+ G+ LGPKGLDW+KIHL+NL G  K+ S   RL+ A  ++ +++DSA +P DGR WW  +++PWQ LA C EI        ++  PD   F S  P+HQDGSCNGLQHYAALG+D  GA +VNL+    PQDVYS V + +E  R  DA+ G++ A +L+G +SRKV+KQTVMT VYGVT+YGG LQI + LK ++  P   +   + YL      S+ E+F     IQ W  E +  I++  +  + W TP+   + QPY    + ILK                  D +K KNAFPPNFIHSLDSTHMMLT+L C    +TFVSVHDC+WTHA  VD MN++CRE+FV LHSQPILE LS +LL+KY  +  P      +K+       K+L ++P+ G FD+ +V DS YFFS
Sbjct:  266 YLEACMFLDDVSRAQNYLLS------HHRQLSKRSQLSISA-YNIVMRMWAKKGSINNIRRLFSLIEEAGMKPNVTSYCAALECMGRMQNCETVVIARCLQQMGADGLSVDDVLSQCVFKKDEREMVLRAVQLVIPHFQPSVSKTQPVCSLPLVKQFYAEREDAIYPKLDYSLKELQDRFNLQLAMEKATTVTINSVEALKPVTENMAHMRELLAKQRKHWEKALLQALKDRKQMMSNNSQKSWNTCLYPYLCILDDQEYVDIMLQSLDKLSPNGE----SLLFMADEMGNRVYNLYCIRQKSQSQMVEKLRSIYSSYAELLANDTETNGMLPRECWTNLEISGNRSGPSLLSDDTPWPMILMVQLGSFLVDLMVHELKIQSNILNPALEKKLIPILYHMYTFRSNHKIGFIKPHPILTRILREATETKLTFDSYVMPMLCPPVPW-TSPKSGAYLLT--PAKLMRASDGAMQHQLLLEKTQEEDLHAVLDSLNQVGSCPWKINKPLLDIIISIFNDKGSDKLSIPPPLSE--APEIPRFNPHDPSYTQVEKAFMKREGIKAKKVVAEMHSLRMDALYKLSIANHVRDKIFWFPHNMDFRGRTYPLPPYFNHLGSDVTRALLLFAEGRPLGPKGLDWLKIHLVNLTGLKKQSSLDGRLEYAESIMEDILDSADHPLDGRKWWMNADEPWQALACCMEI------ANASRSPDHHKFISYFPVHQDGSCNGLQHYAALGRDVIGATSVNLMPCALPQDVYSGVAKQVEEFRVRDAERGLKIAQVLQGFISRKVVKQTVMTVVYGVTRYGGRLQIEKRLKEIDDFPKDYVWDASHYLVVQVFNSLKEMFTGTREIQEWLTESARLIAKSGR-TVEWVTPLGLPIIQPYHRIRNQILKGNIQNLNIHISHDANEKPDSMKQKNAFPPNFIHSLDSTHMMLTSLHCHRAGLTFVSVHDCYWTHAVTVDIMNRVCREQFVALHSQPILEELSWFLLQKYSRR--PPVGSKPKKLLEYYTLMKVLSSVPQTGDFDLEKVKDSVYFFS 1281          
BLAST of DNA-directed RNA polymerase vs. Ensembl Xenopus
Match: pacrg (parkin co-regulated [Source:Xenbase;Acc:XB-GENE-6258806])

HSP 1 Score: 534.258 bits (1375), Expect = 3.125e-169
Identity = 354/1040 (34.04%), Postives = 546/1040 (52.50%), Query Frame = 1
Query:  502 TYMDVCIQKGDYEKALSLFNSVRNENETYHSVDKKSIIVDSVFYEPIMKLLAKNGKIDAIITLLNEMDEFHILPNISIIFRTIEAASKNELLNTEFLHRMMGYVKCHNISLEKAILKAPITKENFSHILKALQMIEPNFNI------ICNKPELKNKSALKETCIFS----SIDELEKFFEVQLNNEILTSVNVDPIHNVTVNPEILKKVILIEDT----WRSSLTEAYEKKITLLEY----SDRSKLYPYLSVLPTKSYVDAMIQVVFQLSTDSE--LQFNRIQHVHLHIGNIISNMFRHRCLKKQKYFAQLKSICLSYYGKLSENPQ--NIEMDKLWESSIMENIDSGSIVH-DTIDWSKKIKASIGKILYEIMFENM---NFTFYNGITAVTTPVIYEKLEEDAGKVFITLKIQSKLRTIFKAINKALPGFSQLDMPMTHPPVPFHKNHESGFFYLTNYATRIIRKSTIFDIFDDDLEQ--PDDISKVYNALNVLSLCSWKIENANLDLVMQVFNDSGNMKLKIP---TSANKFNGPEKPAVDLPIKDKLNSWKSYFQFVKEQKEMYSLWSTLSYKIMIAQKLKNKPFWYPYSMDFRGRVYPCSPHLSHIGDDVSRGLLRFSLGKKLGPKGLDWIKIHLINLCGTHKRKSNSDRLKAANEVISEVIDSAANPFDGRCWWQLSEKPWQTLATCREIHKILKFVKSNGKPDDFHSTLPIHQDGSCNGLQHYXXXXXXXXXXXXVNLIQSETPQDVYSDVLEHLEVKRKEDAKNGVEEAILLEGLLSRKVIKXXXXXXXXXXXXXXXXLQILRELKNLEKLPPQNIKSCAAYLSKLTLGSIGEIFASATNIQNWFIELSSNISRLRKANISWKTPMNFIVKQPYFADDSDILKS-----------------DCLKNKNAFPPNFIHSLDSTHMMLTALSCWEKNITFVSVHDCFWTHACDVDEMNKICREEFVKLHSQPILENLSDYLLEKYQFQINPNENDNGRKIAFNKL-----LKNIPKKGTFDIREVLDSTYFFS 3462
            +Y++VC+   D E+A    N        YH    +  ++    Y  +MK  AK G +  I  L   ++E  + PN+      +E  +++    T+ +   +  ++   +S+ +   + P  ++    IL A+  + PN+ +      +C  P +KN  + +    +     ++ EL   FE Q+  E   ++ +D +  +    E  K+   + D     W+ +L +A+      LE     S R  LYPYL +L    YVD M+Q +  +    E  L  +R       +G  I+N +      ++ +  +++ +   Y   L++N +   +   ++WE    +     S+++ DTI W   +   +G  L ++M + +   N    +       PV+Y      + K    +K       I     ++   F    MPM  PPVP+       +        R +  +    +    LE+  P  +  V ++LN L +C+W+I    LD+++ +FN+ GN KL IP   + A K   PE+P  DL  +DK+   +   +  K+  EM+SL     YK+ IA  L++K FW+P++MDFRGR YPC P+ +H+G DV+R LL F+ G+ LGPKGL+W+K HLINL G  KR S  +R + A+ V+ +++DSA NP  G+ WW  +++PWQ LA C EI         +  P  + S  P+HQDGSCNGLQHYAALG+D  GA +VNL+  + PQDVYS V + +E  RK DA+ GV+ A +L+G + RKV+KQTVMT VYGVT+YGG LQI + L+ L+  P + +   + YL +    S+ E+F+    IQ W  E +  IS+     + W+TP+   + QPY      + +S                 D +K KNAFPPNFIHSLDSTHMMLTAL C+   +TFVSVHDCFWTH   VD MNK+CRE+FV LHSQPIL+NLS +LL+KY   ++   +D   K++   L       N+P+ G FD+++V+DSTYFFS
Sbjct:  197 SYLEVCVFLKDLERAQMCLN-------FYHHRSSRRSLLTIAMYNLLMKAWAKKGSVTNIGHLFMMLEEAGLKPNLGSYAAALECMARSST-ATKSIQMCLSQMEEDGLSINQLFQEFPYQEDERELILNAVHKVHPNYQLPIQPKTVCTSPLVKNFYSKEPPPSYPKLEFTLSELCNRFEEQMAIESCDTITIDSVEAIKPIAEGQKQARDLLDALRARWKKALLQAFRDSKKHLEMKSRISPRVNLYPYLCLLGEDQYVDIMLQSLASIPPTGESFLVMSR------DMGTKINNRYSIHKKLQESHINRIQDLYRDYCQLLAKNGKVSGLLPREMWEQLEQKKYGGASLMNLDTI-WPNSLLVQLGTHLVDLMVQEIKVPNDLLSSRDDPKLIPVLYHMYSFRSTKQIGLIKPHPIFSQIQSDAGESCLTFESSVMPMLCPPVPWLSPRFGAYLLSPAKLMRCMEGAVQHQLL---LEKSPPGQLYPVLDSLNQLGMCAWRINQPILDIIVSIFNEKGNEKLDIPPPLSEAPKL--PEQPHGDLNSRDKMMHQRELARCRKKSSEMHSLRMDALYKLSIASHLRDKIFWFPHNMDFRGRTYPCPPYFNHLGSDVTRALLLFAEGRPLGPKGLEWLKTHLINLTGLKKRNSLQERKEYADSVMDDILDSADNPLKGKRWWMEADEPWQALACCMEIAN----ASRSPDPTKYISHFPVHQDGSCNGLQHYAALGRDEIGAKSVNLMPCDIPQDVYSGVAQQVEEFRKRDAQRGVKIAQILDGFIGRKVVKQTVMTVVYGVTRYGGRLQIEKRLRELDNFPKEYVWEASHYLVQQVFSSLKEMFSGTREIQLWLTESARMISK-SGNTVEWQTPLGLPIIQPYHRTKPVLFQSNLQVVNLQSSHDVNERPDTMKQKNAFPPNFIHSLDSTHMMLTALHCYSHGLTFVSVHDCFWTHPDTVDVMNKVCREQFVALHSQPILQNLSQFLLQKYCQTLS---SDKMAKMSPENLRMTLHFSNVPETGNFDLKQVIDSTYFFS 1208          
BLAST of DNA-directed RNA polymerase vs. Ensembl Xenopus
Match: pacrg (parkin co-regulated [Source:Xenbase;Acc:XB-GENE-6258806])

HSP 1 Score: 533.872 bits (1374), Expect = 6.033e-169
Identity = 354/1040 (34.04%), Postives = 546/1040 (52.50%), Query Frame = 1
Query:  502 TYMDVCIQKGDYEKALSLFNSVRNENETYHSVDKKSIIVDSVFYEPIMKLLAKNGKIDAIITLLNEMDEFHILPNISIIFRTIEAASKNELLNTEFLHRMMGYVKCHNISLEKAILKAPITKENFSHILKALQMIEPNFNI------ICNKPELKNKSALKETCIFS----SIDELEKFFEVQLNNEILTSVNVDPIHNVTVNPEILKKVILIEDT----WRSSLTEAYEKKITLLEY----SDRSKLYPYLSVLPTKSYVDAMIQVVFQLSTDSE--LQFNRIQHVHLHIGNIISNMFRHRCLKKQKYFAQLKSICLSYYGKLSENPQ--NIEMDKLWESSIMENIDSGSIVH-DTIDWSKKIKASIGKILYEIMFENM---NFTFYNGITAVTTPVIYEKLEEDAGKVFITLKIQSKLRTIFKAINKALPGFSQLDMPMTHPPVPFHKNHESGFFYLTNYATRIIRKSTIFDIFDDDLEQ--PDDISKVYNALNVLSLCSWKIENANLDLVMQVFNDSGNMKLKIP---TSANKFNGPEKPAVDLPIKDKLNSWKSYFQFVKEQKEMYSLWSTLSYKIMIAQKLKNKPFWYPYSMDFRGRVYPCSPHLSHIGDDVSRGLLRFSLGKKLGPKGLDWIKIHLINLCGTHKRKSNSDRLKAANEVISEVIDSAANPFDGRCWWQLSEKPWQTLATCREIHKILKFVKSNGKPDDFHSTLPIHQDGSCNGLQHYXXXXXXXXXXXXVNLIQSETPQDVYSDVLEHLEVKRKEDAKNGVEEAILLEGLLSRKVIKXXXXXXXXXXXXXXXXLQILRELKNLEKLPPQNIKSCAAYLSKLTLGSIGEIFASATNIQNWFIELSSNISRLRKANISWKTPMNFIVKQPYFADDSDILKS-----------------DCLKNKNAFPPNFIHSLDSTHMMLTALSCWEKNITFVSVHDCFWTHACDVDEMNKICREEFVKLHSQPILENLSDYLLEKYQFQINPNENDNGRKIAFNKL-----LKNIPKKGTFDIREVLDSTYFFS 3462
            +Y++VC+   D E+A    N        YH    +  ++    Y  +MK  AK G +  I  L   ++E  + PN+      +E  +++    T+ +   +  ++   +S+ +   + P  ++    IL A+  + PN+ +      +C  P +KN  + +    +     ++ EL   FE Q+  E   ++ +D +  +    E  K+   + D     W+ +L +A+      LE     S R  LYPYL +L    YVD M+Q +  +    E  L  +R       +G  I+N +      ++ +  +++ +   Y   L++N +   +   ++WE    +     S+++ DTI W   +   +G  L ++M + +   N    +       PV+Y      + K    +K       I     ++   F    MPM  PPVP+       +        R +  +    +    LE+  P  +  V ++LN L +C+W+I    LD+++ +FN+ GN KL IP   + A K   PE+P  DL  +DK+   +   +  K+  EM+SL     YK+ IA  L++K FW+P++MDFRGR YPC P+ +H+G DV+R LL F+ G+ LGPKGL+W+K HLINL G  KR S  +R + A+ V+ +++DSA NP  G+ WW  +++PWQ LA C EI         +  P  + S  P+HQDGSCNGLQHYAALG+D  GA +VNL+  + PQDVYS V + +E  RK DA+ GV+ A +L+G + RKV+KQTVMT VYGVT+YGG LQI + L+ L+  P + +   + YL +    S+ E+F+    IQ W  E +  IS+     + W+TP+   + QPY      + +S                 D +K KNAFPPNFIHSLDSTHMMLTAL C+   +TFVSVHDCFWTH   VD MNK+CRE+FV LHSQPIL+NLS +LL+KY   ++   +D   K++   L       N+P+ G FD+++V+DSTYFFS
Sbjct:  216 SYLEVCVFLKDLERAQMCLN-------FYHHRSSRRSLLTIAMYNLLMKAWAKKGSVTNIGHLFMMLEEAGLKPNLGSYAAALECMARSSTA-TKSIQMCLSQMEEDGLSINQLFQEFPYQEDERELILNAVHKVHPNYQLPIQPKTVCTSPLVKNFYSKEPPPSYPKLEFTLSELCNRFEEQMAIESCDTITIDSVEAIKPIAEGQKQARDLLDALRARWKKALLQAFRDSKKHLEMKSRISPRVNLYPYLCLLGEDQYVDIMLQSLASIPPTGESFLVMSR------DMGTKINNRYSIHKKLQESHINRIQDLYRDYCQLLAKNGKVSGLLPREMWEQLEQKKYGGASLMNLDTI-WPNSLLVQLGTHLVDLMVQEIKVPNDLLSSRDDPKLIPVLYHMYSFRSTKQIGLIKPHPIFSQIQSDAGESCLTFESSVMPMLCPPVPWLSPRFGAYLLSPAKLMRCMEGAVQHQLL---LEKSPPGQLYPVLDSLNQLGMCAWRINQPILDIIVSIFNEKGNEKLDIPPPLSEAPKL--PEQPHGDLNSRDKMMHQRELARCRKKSSEMHSLRMDALYKLSIASHLRDKIFWFPHNMDFRGRTYPCPPYFNHLGSDVTRALLLFAEGRPLGPKGLEWLKTHLINLTGLKKRNSLQERKEYADSVMDDILDSADNPLKGKRWWMEADEPWQALACCMEIAN----ASRSPDPTKYISHFPVHQDGSCNGLQHYAALGRDEIGAKSVNLMPCDIPQDVYSGVAQQVEEFRKRDAQRGVKIAQILDGFIGRKVVKQTVMTVVYGVTRYGGRLQIEKRLRELDNFPKEYVWEASHYLVQQVFSSLKEMFSGTREIQLWLTESARMISK-SGNTVEWQTPLGLPIIQPYHRTKPVLFQSNLQVVNLQSSHDVNERPDTMKQKNAFPPNFIHSLDSTHMMLTALHCYSHGLTFVSVHDCFWTHPDTVDVMNKVCREQFVALHSQPILQNLSQFLLQKYCQTLS---SDKMAKMSPENLRMTLHFSNVPETGNFDLKQVIDSTYFFS 1227          
BLAST of DNA-directed RNA polymerase vs. Ensembl Xenopus
Match: pacrg (parkin co-regulated [Source:Xenbase;Acc:XB-GENE-6258806])

HSP 1 Score: 514.612 bits (1324), Expect = 3.987e-162
Identity = 348/1030 (33.79%), Postives = 533/1030 (51.75%), Query Frame = 1
Query:  502 TYMDVCIQKGDYEKALSLFNSVRNENETYHSVDKKSIIVDSVFYEPIMKLLAKNGKIDAIITLLNEMDEFHILPNISIIFRTIEAASKNELLNTEFLHRMMGYVKCHNISLEKAILKAPITKENFSHILKALQMIEPNFNI------ICNKPELKNKSALKETCIFS----SIDELEKFFEVQLNNEILTSVNVDPIHNVTVNPEILKKVILIEDT----WRSSLTEAYEKKITLLEY----SDRSKLYPYLSVLPTKSYVDAMIQVVFQLSTDSE--LQFNRIQHVHLHIGNIISNMFRHRCLKKQKYFAQLKSICLSYYGKLSENPQNIEMDKLWESSIMENIDSGSIVHDTIDWSKKIKASI-----GKILYEIMFENMNFTFYNGITAVTTPVIYEKLEEDAGKVFITLKIQSKLRTIFKAINKALPGFSQLDMPMTHPPVPFHKNHESGFFYLTNYATRIIRKSTIFDIFDDDLEQPDDISKVYNALNVLSLCSWKIENANLDLVMQVFNDSGNMKLKIP---TSANKFNGPEKPAVDLPIKDKLNSWKSYFQFVKEQKEMYSLWSTLSYKIMIAQKLKNKPFWYPYSMDFRGRVYPCSPHLSHIGDDVSRGLLRFSLGKKLGPKGLDWIKIHLINLCGTHKRKSNSDRLKAANEVISEVIDSAANPFDGRCWWQLSEKPWQTLATCREIHKILKFVKSNGKPDDFHSTLPIHQDGSCNGLQHYXXXXXXXXXXXXVNLIQSETPQDVYSDVLEHLEVKRKEDAKNGVEEAILLEGLLSRKVIKXXXXXXXXXXXXXXXXLQILRELKNLEKLPPQNIKSCAAYLSKLTLGSIGEIFASATNIQNWFIELSSNISRLRKANISWKTPMNFIVKQPY--------------FADDSDI-LKSDCLKNKNAFPPNFIHSLDSTHMMLTALSCWEKNITFVSVHDCFWTHACDVDEMNKICREEFVKLHSQPILENLSDYLLEKYQFQINPNENDNGRKIAFNKLLKNIPKKGTFDIREVLDSTYFFS 3462
            +Y++VC+   D E+A    N        YH    +  ++    Y  +MK  AK G +  I  L   ++E  + PN+      +E  +++    T  + R +  ++   +S+ +   + P  ++    IL A+  + PN+ +      +C  P +KN  + +    +     ++ EL   FE Q+  E   ++ +D +  +    E  K+   + D     W+ +L +A+      LE     S R  LYPYL +L    YVD M+Q +  +    E  L  +R             +M +   L  ++ + QL+      YG  S     + +D +W +S++  +  G+ + D +    K+   +        L  +++   +F     I  +    I+ +++ DAG+  +T                    F    MPM  PPVP+       +        R +  +    +  +    P  +  V ++LN L +C+W+I    LD+++ +FN+ GN KL IP   + A K   PE+P  DL  +DK+   +   +  K+  EM+SL     YK+ IA  L++K FW+P++MDFRGR YPC P+ +H+G DV+R LL F+ G+ LGPKGL+W+K HLINL G  KR S  +R + A+ V+ +++DSA NP  G+ WW  +++PWQ LA C EI         +  P  + S  P+HQDGSCNGLQHYAALG+D  GA +VNL+  + PQDVYS V + +E  RK DA+ GV+ A +L+G + RKV+KQTVMT VYGVT+YGG LQI + L+ L+  P + +   + YL +    S+ E+F+    IQ W  E +  IS+     + W+TP+   + QPY                   D+  + D +K KNAFPPNFIHSLDSTHMMLTAL C+   +TFVSVHDCFWTH   VD MNK+CRE+FV LHSQPIL+NLS +LL+KY   ++       R +    L   +   G FD+++V+DSTYFFS
Sbjct:  230 SYLEVCVFLKDLERAQMCLN-------FYHHRSSRRSLLTIAMYNLLMKAWAKKGSVTNIGHLFMMLEEAGLKPNLGSYAAALECMARS----TFCIFRCLSQMEEDGLSINQLFQEFPYQEDERELILNAVHKVHPNYQLPIQPKTVCTSPLVKNFYSKEPPPSYPKLEFTLSELCNRFEEQMAIESCDTITIDSVEAIKPIAEGQKQARDLLDALRARWKKALLQAFRDSKKHLEMKSRISPRVNLYPYLCLLGEDQYVDIMLQSLASIPPTGESFLVMSR-------------DMDKVSGLLPREMWEQLEQ---KKYGGASL----MNLDTIWPNSLL--VQLGTHLVDLMVQEIKVPNDLLSSRDDPKLIPVLYHMYSFRSTKQIGLIKPHPIFSQIQSDAGESCLT--------------------FESSVMPMLCPPVPWLSPRFGAYLLSPAKLMRCMEGAVQHQLLLEK-SPPGQLYPVLDSLNQLGMCAWRINQPILDIIVSIFNEKGNEKLDIPPPLSEAPKL--PEQPHGDLNSRDKMMHQRELARCRKKSSEMHSLRMDALYKLSIASHLRDKIFWFPHNMDFRGRTYPCPPYFNHLGSDVTRALLLFAEGRPLGPKGLEWLKTHLINLTGLKKRNSLQERKEYADSVMDDILDSADNPLKGKRWWMEADEPWQALACCMEIAN----ASRSPDPTKYISHFPVHQDGSCNGLQHYAALGRDEIGAKSVNLMPCDIPQDVYSGVAQQVEEFRKRDAQRGVKIAQILDGFIGRKVVKQTVMTVVYGVTRYGGRLQIEKRLRELDNFPKEYVWEASHYLVQQVFSSLKEMFSGTREIQLWLTESARMISK-SGNTVEWQTPLGLPIIQPYHRTKPVLSNLQVVNLQSSHDVNERPDTMKQKNAFPPNFIHSLDSTHMMLTALHCYSHGLTFVSVHDCFWTHPDTVDVMNKVCREQFVALHSQPILQNLSQFLLQKYCQTLS-------RPMIILFLCFIV---GNFDLKQVIDSTYFFS 1188          
BLAST of DNA-directed RNA polymerase vs. Ensembl Xenopus
Match: pacrg (parkin co-regulated [Source:Xenbase;Acc:XB-GENE-6258806])

HSP 1 Score: 511.146 bits (1315), Expect = 6.429e-161
Identity = 336/990 (33.94%), Postives = 517/990 (52.22%), Query Frame = 1
Query:  502 TYMDVCIQKGDYEKALSLFNSVRNENETYHSVDKKSIIVDSVFYEPIMKLLAKNGKIDAIITLLNEMDEFHILPNISIIFRTIEAASKNELLNTEFLHRMMGYVKCHNISLEKAILKAPITKENFSHILKALQMIEPNFNI------ICNKPELKNKSALKETCIFS----SIDELEKFFEVQLNNEILTSVNVDPIHNVTVNPEILKKVILIEDT----WRSSLTEAYEKKITLLEY----SDRSKLYPYLSVLPTKSYVDAMIQVVFQLSTDSE--LQFNRIQHVHLHIGNIISNMFRHRCLKKQKYFAQLKSICLSYYGKLSENPQ--NIEMDKLWESSIMENIDSGSIVH-DTIDWSKKIKASIGKILYEIMFENM---NFTFYNGITAVTTPVIYEKLEEDAGKVFITLKIQSKLRTIFKAINKALPGFSQLDMPMTHPPVPFHKNHESGFFYLTNYATRIIRKSTIFDIFDDDLEQPDDISKVYNALNVLSLCSWKIENANLDLVMQVFNDSGNMKLKIP---TSANKFNGPEKPAVDLPIKDKLNSWKSYFQFVKEQKEMYSLWSTLSYKIMIAQKLKNKPFWYPYSMDFRGRVYPCSPHLSHIGDDVSRGLLRFSLGKKLGPKGLDWIKIHLINLCGTHKRKSNSDRLKAANEVISEVIDSAANPFDGRCWWQLSEKPWQTLATCREIHKILKFVKSNGKPDDFHSTLPIHQDGSCNGLQHYXXXXXXXXXXXXVNLIQSETPQDVYSDVLEHLEVKRKEDAKNGVEEAILLEGLLSRKVIKXXXXXXXXXXXXXXXXLQILRELKNLEKLPPQNIKSCAAYLSKLTLGSIGEIFASATNIQNWFIELSSNISRLRKANISWKTPMNFIVKQPYFADDSDILKS-----------------DCLKNKNAFPPNFIHSLDSTHMMLTALSCWEKNITFVSVHDCFWTHACDVDEMNKICREEFVKLHSQPILENLSDYLLEKY 3333
            +Y++VC+   D E+A    N        YH    +  ++    Y  +MK  AK G +  I  L   ++E  + PN+      +E  +++    T+ +   +  ++   +S+ +   + P  ++    IL A+  + PN+ +      +C  P +KN  + +    +     ++ EL   FE Q+  E   ++ +D +  +    E  K+   + D     W+ +L +A+      LE     S R  LYPYL +L    YVD M+Q +  +    E  L  +R       +G  I+N +      ++ +  +++ +   Y   L++N +   +   ++WE    +     S+++ DTI W   +   +G  L ++M + +   N    +       PV+Y      + K    +K       I     ++   F    MPM  PPVP+       +        R +  +    +  +    P  +  V ++LN L +C+W+I    LD+++ +FN+ GN KL IP   + A K   PE+P  DL  +DK+   +   +  K+  EM+SL     YK+ IA  L++K FW+P++MDFRGR YPC P+ +H+G DV+R LL F+ G+ LGPKGL+W+K HLINL G  KR S  +R + A+ V+ +++DSA NP  G+ WW  +++PWQ LA C EI         +  P  + S  P+HQDGSCNGLQHYAALG+D  GA +VNL+  + PQDVYS V + +E  RK DA+ GV+ A +L+G + RKV+KQTVMT VYGVT+YGG LQI + L+ L+  P + +   + YL +    S+ E+F+    IQ W  E +  IS+     + W+TP+   + QPY      + +S                 D +K KNAFPPNFIHSLDSTHMMLTAL C+   +TFVSVHDCFWTH   VD MNK+CRE+FV LHSQPIL+NLS +LL+KY
Sbjct:  197 SYLEVCVFLKDLERAQMCLN-------FYHHRSSRRSLLTIAMYNLLMKAWAKKGSVTNIGHLFMMLEEAGLKPNLGSYAAALECMARSST-ATKSIQMCLSQMEEDGLSINQLFQEFPYQEDERELILNAVHKVHPNYQLPIQPKTVCTSPLVKNFYSKEPPPSYPKLEFTLSELCNRFEEQMAIESCDTITIDSVEAIKPIAEGQKQARDLLDALRARWKKALLQAFRDSKKHLEMKSRISPRVNLYPYLCLLGEDQYVDIMLQSLASIPPTGESFLVMSR------DMGTKINNRYSIHKKLQESHINRIQDLYRDYCQLLAKNGKVSGLLPREMWEQLEQKKYGGASLMNLDTI-WPNSLLVQLGTHLVDLMVQEIKVPNDLLSSRDDPKLIPVLYHMYSFRSTKQIGLIKPHPIFSQIQSDAGESCLTFESSVMPMLCPPVPWLSPRFGAYLLSPAKLMRCMEGAVQHQLLLEK-SPPGQLYPVLDSLNQLGMCAWRINQPILDIIVSIFNEKGNEKLDIPPPLSEAPKL--PEQPHGDLNSRDKMMHQRELARCRKKSSEMHSLRMDALYKLSIASHLRDKIFWFPHNMDFRGRTYPCPPYFNHLGSDVTRALLLFAEGRPLGPKGLEWLKTHLINLTGLKKRNSLQERKEYADSVMDDILDSADNPLKGKRWWMEADEPWQALACCMEIAN----ASRSPDPTKYISHFPVHQDGSCNGLQHYAALGRDEIGAKSVNLMPCDIPQDVYSGVAQQVEEFRKRDAQRGVKIAQILDGFIGRKVVKQTVMTVVYGVTRYGGRLQIEKRLRELDNFPKEYVWEASHYLVQQVFSSLKEMFSGTREIQLWLTESARMISK-SGNTVEWQTPLGLPIIQPYHRTKPVLFQSNLQVVNLQSSHDVNERPDTMKQKNAFPPNFIHSLDSTHMMLTALHCYSHGLTFVSVHDCFWTHPDTVDVMNKVCREQFVALHSQPILQNLSQFLLQKY 1163          
BLAST of DNA-directed RNA polymerase vs. Ensembl Xenopus
Match: pacrg (parkin co-regulated [Source:Xenbase;Acc:XB-GENE-6258806])

HSP 1 Score: 511.146 bits (1315), Expect = 1.023e-160
Identity = 338/992 (34.07%), Postives = 519/992 (52.32%), Query Frame = 1
Query:  502 TYMDVCIQKGDYEKALSLFNSVRNENETYHSVDKKSIIVDSVFYEPIMKLLAKNGKIDAIITLLNEMDEFHILPNISIIFRTIEAASKNELLNTEFLHRMMGYVKCHNISLEKAILKAPITKENFSHILKALQMIEPNFNI------ICNKPELKNKSALKETCIFS----SIDELEKFFEVQLNNEILTSVNVDPIHNVTVNPEILKKVILIEDT----WRSSLTEAYEKKITLLEY----SDRSKLYPYLSVLPTKSYVDAMIQVVFQLSTDSE--LQFNRIQHVHLHIGNIISNMFRHRCLKKQKYFAQLKSICLSYYGKLSENPQ--NIEMDKLWESSIMENIDSGSIVH-DTIDWSKKIKASIGKILYEIMFENM---NFTFYNGITAVTTPVIYEKLEEDAGKVFITLKIQSKLRTIFKAINKALPGFSQLDMPMTHPPVPFHKNHESGFFYLTNYATRIIRKSTIFDIFDDDLEQ--PDDISKVYNALNVLSLCSWKIENANLDLVMQVFNDSGNMKLKIP---TSANKFNGPEKPAVDLPIKDKLNSWKSYFQFVKEQKEMYSLWSTLSYKIMIAQKLKNKPFWYPYSMDFRGRVYPCSPHLSHIGDDVSRGLLRFSLGKKLGPKGLDWIKIHLINLCGTHKRKSNSDRLKAANEVISEVIDSAANPFDGRCWWQLSEKPWQTLATCREIHKILKFVKSNGKPDDFHSTLPIHQDGSCNGLQHYXXXXXXXXXXXXVNLIQSETPQDVYSDVLEHLEVKRKEDAKNGVEEAILLEGLLSRKVIKXXXXXXXXXXXXXXXXLQILRELKNLEKLPPQNIKSCAAYLSKLTLGSIGEIFASATNIQNWFIELSSNISRLRKANISWKTPMNFIVKQPYFADDSDILKS-----------------DCLKNKNAFPPNFIHSLDSTHMMLTALSCWEKNITFVSVHDCFWTHACDVDEMNKICREEFVKLHSQPILENLSDYLLEKY 3333
            +Y++VC+   D E+A    N        YH    +  ++    Y  +MK  AK G +  I  L   ++E  + PN+      +E  +++    T+ +   +  ++   +S+ +   + P  ++    IL A+  + PN+ +      +C  P +KN  + +    +     ++ EL   FE Q+  E   ++ +D +  +    E  K+   + D     W+ +L +A+      LE     S R  LYPYL +L    YVD M+Q +  +    E  L  +R       +G  I+N +      ++ +  +++ +   Y   L++N +   +   ++WE    +     S+++ DTI W   +   +G  L ++M + +   N    +       PV+Y      + K    +K       I     ++   F    MPM  PPVP+       +        R +  +    +    LE+  P  +  V ++LN L +C+W+I    LD+++ +FN+ GN KL IP   + A K   PE+P  DL  +DK+   +   +  K+  EM+SL     YK+ IA  L++K FW+P++MDFRGR YPC P+ +H+G DV+R LL F+ G+ LGPKGL+W+K HLINL G  KR S  +R + A+ V+ +++DSA NP  G+ WW  +++PWQ LA C EI         +  P  + S  P+HQDGSCNGLQHYAALG+D  GA +VNL+  + PQDVYS V + +E  RK DA+ GV+ A +L+G + RKV+KQTVMT VYGVT+YGG LQI + L+ L+  P + +   + YL +    S+ E+F+    IQ W  E +  IS+     + W+TP+   + QPY      + +S                 D +K KNAFPPNFIHSLDSTHMMLTAL C+   +TFVSVHDCFWTH   VD MNK+CRE+FV LHSQPIL+NLS +LL+KY
Sbjct:  197 SYLEVCVFLKDLERAQMCLN-------FYHHRSSRRSLLTIAMYNLLMKAWAKKGSVTNIGHLFMMLEEAGLKPNLGSYAAALECMARSSTA-TKSIQMCLSQMEEDGLSINQLFQEFPYQEDERELILNAVHKVHPNYQLPIQPKTVCTSPLVKNFYSKEPPPSYPKLEFTLSELCNRFEEQMAIESCDTITIDSVEAIKPIAEGQKQARDLLDALRARWKKALLQAFRDSKKHLEMKSRISPRVNLYPYLCLLGEDQYVDIMLQSLASIPPTGESFLVMSR------DMGTKINNRYSIHKKLQESHINRIQDLYRDYCQLLAKNGKVSGLLPREMWEQLEQKKYGGASLMNLDTI-WPNSLLVQLGTHLVDLMVQEIKVPNDLLSSRDDPKLIPVLYHMYSFRSTKQIGLIKPHPIFSQIQSDAGESCLTFESSVMPMLCPPVPWLSPRFGAYLLSPAKLMRCMEGAVQHQLL---LEKSPPGQLYPVLDSLNQLGMCAWRINQPILDIIVSIFNEKGNEKLDIPPPLSEAPKL--PEQPHGDLNSRDKMMHQRELARCRKKSSEMHSLRMDALYKLSIASHLRDKIFWFPHNMDFRGRTYPCPPYFNHLGSDVTRALLLFAEGRPLGPKGLEWLKTHLINLTGLKKRNSLQERKEYADSVMDDILDSADNPLKGKRWWMEADEPWQALACCMEIAN----ASRSPDPTKYISHFPVHQDGSCNGLQHYAALGRDEIGAKSVNLMPCDIPQDVYSGVAQQVEEFRKRDAQRGVKIAQILDGFIGRKVVKQTVMTVVYGVTRYGGRLQIEKRLRELDNFPKEYVWEASHYLVQQVFSSLKEMFSGTREIQLWLTESARMISK-SGNTVEWQTPLGLPIIQPYHRTKPVLFQSNLQVVNLQSSHDVNERPDTMKQKNAFPPNFIHSLDSTHMMLTALHCYSHGLTFVSVHDCFWTHPDTVDVMNKVCREQFVALHSQPILQNLSQFLLQKY 1163          
BLAST of DNA-directed RNA polymerase vs. Ensembl Mouse
Match: Polrmt (polymerase (RNA) mitochondrial (DNA directed) [Source:MGI Symbol;Acc:MGI:1915843])

HSP 1 Score: 499.204 bits (1284), Expect = 3.584e-156
Identity = 340/1013 (33.56%), Postives = 528/1013 (52.12%), Query Frame = 1
Query:  583 TYHSVDKKSIIVDSVFYEPIMKLLAKNGKIDAIITLLNEMDEFHILPNISIIFRTIEAASKNELLNTEFLHRMMGYVKCHNISLEKAILKAPITKENFSHILKALQMIEPNFNIICNKPELKNKSALKETCIFS------------SIDELEKFFEVQLNNEILTSVNVDPIHNVTVNP----EILKKVILIEDTWRSSLTEAY-EKKITLLE--YSDRSKLYPYLSVLPTKSYVDAMIQVVFQLSTDSELQFNRIQHVHLHIGNIISNMFRHRCLKKQ--KYFAQLKSICLSYYGKLSENPQNIEM--DKLWESSIMENIDSGSIVHDTIDWSKKIKASIGKILYEIMFENM----NFTFYNGITAVTTPVIYEKLEEDAGKVFITLKIQSKLRTIFKAINKALPGFSQLDMPMTHPPVPFHKNHESGFFYLTNYATRIIRKSTIFDIFDDDLEQ--PDDISKVYNALNVLSLCSWKIENANLDLVMQVFNDSGNMKLKIPTSANKFNGPEK---PAVDLPI-KDKLNSWKSYFQFVKEQKEMYSLWSTLSYKIMIAQKLKNKPFWYPYSMDFRGRVYPCSPHLSHIGDDVSRGLLRFSLGKKLGPKGLDWIKIHLINLCGTHKRKSNSDRLKAANEVISEVIDSAANPFDGRCWWQLSEKPWQTLATCREIHKILKFVKSNGKPDDFHSTLPIHQDGSCNGLQHYXXXXXXXXXXXXVNLIQSETPQDVYSDVLEHLEVKRKEDAKNGVEEAILLEGLLSRKVIKXXXXXXXXXXXXXXXXLQILRELKNLEKLPPQNIKSCAAYLSKLTLGSIGEIFASATNIQNWFIELSSNISRLRKANISWKTPMNFIVKQPYFADDS-----------------DILKSDCLKNKNAFPPNFIHSLDSTHMMLTALSCWEKNITFVSVHDCFWTHACDVDEMNKICREEFVKLHSQPILENLSDYLLEKYQFQINPNENDNGRKIAFNKL---LKNIPKKGTFDIREVLDSTYFFS 3462
            T+H+   +  ++    Y  +M   A+ G    ++ +   + +  + P++      ++   + +  +   + R +  +       +       + +E+ + +L+A+   EP F      P   N S L +  I+S             +D L+  F  QL+ E+ +SV V  +    V      E  K +  + + W   L     E K T+    Y  +  LYP+L +L    +V  ++QV+  L    E       ++ L + N      RH   +KQ   +  +L      Y   L+ + Q       + WES  +  +++ +       WS  +   +GK L E++ + +    +     G    + PV+Y      + +    LK       + +   +    F   ++PM  PP+P+   H SG + L++  T+++R +         LEQ  P  +    +AL  L  C+W++    LDLV+Q+F D G M L +P   ++   P +   P    P+ K +L   K   + +K  +EM+SL S   Y++ +AQ L+++ FW P++MDFRGR YPC PH +H+G D++R LL F+ G+ LGP+GLDW+KIHLINL G  K  S   RL  A+EV+ E++DSA NP  GR WW  +++PWQTLA C E+   ++    +  P  + S LP+HQDGSCNGLQHYAALG+D+ GAA+VNL  S+ PQDVY +V   +E  R++DAK G+  A +LEG +SRKV+KQTVMT VYGVT+YGG LQI + L+ L   P + +   + YL +    S+ E+F S   IQ+W  E S+N+       + W TP+   + QPY  +                   +  K + LK KN FPPNFIHSLDS+HMMLTAL C+ K + FVSVHDCFWTHA D+  MN++CRE+FV+LHSQPILE+L+ +L +++    +     +  +    KL   L+++PK GTFD+ +V+ STYFFS
Sbjct:  220 THHNNGDRQQVLTLHMYNTVMLGWARKGSFRELVYVFLMLKDAGLSPDLCSYAAALQCMGRRDQ-DVRTIQRCLKQMMEEGFQPQLLFTDLVLEEEDRAALLRAVVKAEPAFRPPPQAPSPVNTSTLLKD-IYSKEGPVSYPKLHLPLDTLQDLFYQQLHVELSSSVCVQSVEKAPVMSKEVIEARKTLQALREQWEVELLRVLRETKATMGRQAYEGQPTLYPFLCLLSEGEFVSILMQVLKVLPAQGEPLIQLAHNLGLRVLN------RHLVKQKQVTNHVQKLGQRYSQYLQLLASDTQVAPCLPREYWES--LGPLEAPA----QQPWSVPVLLQLGKQLAELLVQAVQMPRSLAARQGAQR-SIPVLYHVYSFRSYRQVGILKPHPAFTHLLETAAEPTLTFETTEVPMLCPPLPWTSLH-SGAYLLSS--TKLMRATEGTTQHQRLLEQCPPAQLHGPLDALTQLGNCAWRVNGHLLDLVLQIFRDKGCMPLGVPPPRSEAPRPARYQLPPGSTPVHKSELR--KELARCLKVAREMHSLRSEALYRLSLAQHLRHRVFWLPHNMDFRGRTYPCPPHFNHLGSDLARALLEFAEGRPLGPRGLDWLKIHLINLTGLKKGDSLRMRLAFADEVMEEILDSADNPLTGRKWWMEADEPWQTLACCMEVAHAVR----SPDPAAYISHLPVHQDGSCNGLQHYAALGRDSVGAASVNLTPSDLPQDVYREVATQVEEFRQQDAKEGLRVAQVLEGFISRKVVKQTVMTVVYGVTRYGGRLQIEKRLRELSDFPQEFVWEASHYLVRQVFKSLQEMFTSTRAIQHWLTE-SANLISHAGWPVEWVTPLGIPIIQPYHRESKVQVKGGLQSITLTSSVDESQKPNTLKQKNGFPPNFIHSLDSSHMMLTALHCYRKGLIFVSVHDCFWTHAADIPTMNEVCREQFVRLHSQPILEDLAKFLKKRFCSVSSIKSLKSSERALVTKLQETLQSLPKTGTFDLGQVIRSTYFFS 1207          
BLAST of DNA-directed RNA polymerase vs. Ensembl Mouse
Match: Polrmt (polymerase (RNA) mitochondrial (DNA directed) [Source:MGI Symbol;Acc:MGI:1915843])

HSP 1 Score: 468.774 bits (1205), Expect = 1.491e-145
Identity = 267/606 (44.06%), Postives = 377/606 (62.21%), Query Frame = 1
Query: 1723 FSQLDMPMTHPPVPFHKNHESGFFYLTNYATRIIRKSTIFDIFDDDLEQ--PDDISKVYNALNVLSLCSWKIENANLDLVMQVFNDSGNMKLKIPTSANKFNGPEK---PAVDLPI-KDKLNSWKSYFQFVKEQKEMYSLWSTLSYKIMIAQKLKNKPFWYPYSMDFRGRVYPCSPHLSHIGDDVSRGLLRFSLGKKLGPKGLDWIKIHLINLCGTHKRKSNSDRLKAANEVISEVIDSAANPFDGRCWWQLSEKPWQTLATCREIHKILKFVKSNGKPDDFHSTLPIHQDGSCNGLQHYXXXXXXXXXXXXVNLIQSETPQDVYSDVLEHLEVKRKEDAKNGVEEAILLEGLLSRKVIKXXXXXXXXXXXXXXXXLQILRELKNLEKLPPQNIKSCAAYLSKLTLGSIGEIFASATNIQNWFIELSSNISRLRKANISWKTPMNFIVKQPYFADDS-----------------DILKSDCLKNKNAFPPNFIHSLDSTHMMLTALSCWEKNITFVSVHDCFWTHACDVDEMNKICREEFVKLHSQPILENLSDYLLEKYQFQINPNENDNGRKIAFNKL---LKNIPKKGTFDIREVLDSTYFFS 3462
            F   ++PM  PP+P+   H SG + L++  T+++R +         LEQ  P  +    +AL  L  C+W++    LDLV+Q+F D G M L +P   ++   P +   P    P+ K +L   K   + +K  +EM+SL S   Y++ +AQ L+++ FW P++MDFRGR YPC PH +H+G D++R LL F+ G+ LGP+GLDW+KIHLINL G  K  S   RL  A+EV+ E++DSA NP  GR WW  +++PWQTLA C E+   ++    +  P  + S LP+HQDGSCNGLQHYAALG+D+ GAA+VNL  S+ PQDVY +V   +E  R++DAK G+  A +LEG +SRKV+KQTVMT VYGVT+YGG LQI + L+ L   P + +   + YL +    S+ E+F S   IQ+W  E S+N+       + W TP+   + QPY  +                   +  K + LK KN FPPNFIHSLDS+HMMLTAL C+ K + FVSVHDCFWTHA D+  MN++CRE+FV+LHSQPILE+L+ +L +++    +     +  +    KL   L+++PK GTFD+ +V+ STYFFS
Sbjct:  538 FETTEVPMLCPPLPWTSLH-SGAYLLSS--TKLMRATEGTTQHQRLLEQCPPAQLHGPLDALTQLGNCAWRVNGHLLDLVLQIFRDKGCMPLGVPPPRSEAPRPARYQLPPGSTPVHKSELR--KELARCLKVAREMHSLRSEALYRLSLAQHLRHRVFWLPHNMDFRGRTYPCPPHFNHLGSDLARALLEFAEGRPLGPRGLDWLKIHLINLTGLKKGDSLRMRLAFADEVMEEILDSADNPLTGRKWWMEADEPWQTLACCMEVAHAVR----SPDPAAYISHLPVHQDGSCNGLQHYAALGRDSVGAASVNLTPSDLPQDVYREVATQVEEFRQQDAKEGLRVAQVLEGFISRKVVKQTVMTVVYGVTRYGGRLQIEKRLRELSDFPQEFVWEASHYLVRQVFKSLQEMFTSTRAIQHWLTE-SANLISHAGWPVEWVTPLGIPIIQPYHRESKVQVKGGLQSITLTSSVDESQKPNTLKQKNGFPPNFIHSLDSSHMMLTALHCYRKGLIFVSVHDCFWTHAADIPTMNEVCREQFVRLHSQPILEDLAKFLKKRFCSVSSIKSLKSSERALVTKLQETLQSLPKTGTFDLGQVIRSTYFFS 1133          
BLAST of DNA-directed RNA polymerase vs. Ensembl Mouse
Match: Polrmt (polymerase (RNA) mitochondrial (DNA directed) [Source:MGI Symbol;Acc:MGI:1915843])

HSP 1 Score: 321.627 bits (823), Expect = 3.023e-99
Identity = 184/438 (42.01%), Postives = 253/438 (57.76%), Query Frame = 1
Query: 2209 GRVYPCSPHLSHIGDDVSRGLLRFSLGKKLGPKGLDWIKIHLINLCGTHKRKSNSDRLKAANEVISEVIDSAANPFDGRCWWQLSEKPWQTLATCREIHKILKFVKSNGKPDDFHSTLPIHQDGSCNGLQHYXXXXXXXXXXXXVNLIQSETPQDVYSDVLEHLEVKRKEDAKNGVEEAILLEGLLSRKVIKXXXXXXXXXXXXXXXXLQILRELKNLEKLPPQNIKSCAAYLSKLTLGSIGEIFASATNIQNWFIELSSNISRLRKANISWKTPMNFIVKQPYFADDS-----------------DILKSDCLKNKNAFPPNFIHSLDSTHMMLTALSCWEKNITFVSVHDCFWTHACDVDEMNKICREEFVKLHSQPILENLSDYLLEKYQFQINPNENDNGRKIAFNKL---LKNIPKKGTFDIREVLDSTYFFS 3462
            GR YPC PH +H+G D++R LL F+ G+ LGP+GLDW+KIHLINL G  K  S   RL  A+EV+ E++DSA NP  GR WW  +++PWQTLA C E+   ++    +  P  + S LP+HQ                                         +E  R++DAK G+  A +LEG +SRKV+KQTVMT VYGVT+YGG LQI + L+ L   P + +   + YL +    S+ E+F S   IQ+W  E S+N+       + W TP+   + QPY  +                   +  K + LK KN FPPNFIHSLDS+HMMLTAL C+ K + FVSVHDCFWTHA D+  MN++CRE+FV+LHSQPILE+L+ +L +++    +     +  +    KL   L+++PK GTFD+ +V+ STYFFS
Sbjct:    2 GRTYPCPPHFNHLGSDLARALLEFAEGRPLGPRGLDWLKIHLINLTGLKKGDSLRMRLAFADEVMEEILDSADNPLTGRKWWMEADEPWQTLACCMEVAHAVR----SPDPAAYISHLPVHQ-----------------------------------------VEEFRQQDAKEGLRVAQVLEGFISRKVVKQTVMTVVYGVTRYGGRLQIEKRLRELSDFPQEFVWEASHYLVRQVFKSLQEMFTSTRAIQHWLTE-SANLISHAGWPVEWVTPLGIPIIQPYHRESKVQVKGGLQSITLTSSVDESQKPNTLKQKNGFPPNFIHSLDSSHMMLTALHCYRKGLIFVSVHDCFWTHAADIPTMNEVCREQFVRLHSQPILEDLAKFLKKRFCSVSSIKSLKSSERALVTKLQETLQSLPKTGTFDLGQVIRSTYFFS 393          
BLAST of DNA-directed RNA polymerase vs. Ensembl Mouse
Match: Polrmt (polymerase (RNA) mitochondrial (DNA directed) [Source:MGI Symbol;Acc:MGI:1915843])

HSP 1 Score: 252.292 bits (643), Expect = 2.214e-69
Identity = 189/697 (27.12%), Postives = 324/697 (46.48%), Query Frame = 1
Query:  583 TYHSVDKKSIIVDSVFYEPIMKLLAKNGKIDAIITLLNEMDEFHILPNISIIFRTIEAASKNELLNTEFLHRMMGYVKCHNISLEKAILKAPITKENFSHILKALQMIEPNFNIICNKPELKNKSALKETCIFS------------SIDELEKFFEVQLNNEILTSVNVDPIHNVTVNP----EILKKVILIEDTWRSSLTEAY-EKKITLLE--YSDRSKLYPYLSVLPTKSYVDAMIQVVFQLSTDSELQFNRIQHVHLHIGNIISNMFRHRCLKKQ--KYFAQLKSICLSYYGKLSENPQNIEM--DKLWESSIMENIDSGSIVHDTIDWSKKIKASIGKILYEIMFENM----NFTFYNGITAVTTPVIYEKLEEDAGKVFITLKIQSKLRTIFKAINKALPGFSQLDMPMTHPPVPFHKNHESGFFYLTNYATRIIRKSTIFDIFDDDLEQ--PDDISKVYNALNVLSLCSWKIENANLDLVMQVFNDSGNMKLKIPTSANKFNGPEK---PAVDLPI-KDKLNSWKSYFQFVKEQKEMYSLWSTLSYKIMIAQKLKNKPFWYPYSMDFRGRVYPCSPHLSHIGDDVSRGLLRFSLGKKLGPKGLDWIKIHLINLCGTHKRKSNSDRLKAANEVISEVIDSAANPFDGRCWWQLSEKPWQTLATCREIHKILKFVKSNGKPDDFHSTLPIHQ 2574
            T+H+   +  ++    Y  +M   A+ G    ++ +   + +  + P++      ++   + +  +   + R +  +       +       + +E+ + +L+A+   EP F      P   N S L +  I+S             +D L+  F  QL+ E+ +SV V  +    V      E  K +  + + W   L     E K T+    Y  +  LYP+L +L    +V  ++QV+  L    E       ++ L + N      RH   +KQ   +  +L      Y   L+ + Q       + WES  +  +++ +       WS  +   +GK L E++ + +    +     G    + PV+Y      + +    LK       + +   +    F   ++PM  PP+P+   H SG + L++  T+++R +         LEQ  P  +    +AL  L  C+W++    LDLV+Q+F D G M L +P   ++   P +   P    P+ K +L   K   + +K  +EM+SL S   Y++ +AQ L+++ FW P++MDFRGR YPC PH +H+G D++R LL F+ G+ LGP+GLDW+KIHLINL G  K  S   RL  A+EV+ E++DSA NP  GR WW  +++PWQTLA C E+   ++    +  P  + S LP+HQ
Sbjct:  220 THHNNGDRQQVLTLHMYNTVMLGWARKGSFRELVYVFLMLKDAGLSPDLCSYAAALQCMGRRDQ-DVRTIQRCLKQMMEEGFQPQLLFTDLVLEEEDRAALLRAVVKAEPAFRPPPQAPSPVNTSTLLKD-IYSKEGPVSYPKLHLPLDTLQDLFYQQLHVELSSSVCVQSVEKAPVMSKEVIEARKTLQALREQWEVELLRVLRETKATMGRQAYEGQPTLYPFLCLLSEGEFVSILMQVLKVLPAQGEPLIQLAHNLGLRVLN------RHLVKQKQVTNHVQKLGQRYSQYLQLLASDTQVAPCLPREYWES--LGPLEAPA----QQPWSVPVLLQLGKQLAELLVQAVQMPRSLAARQGAQR-SIPVLYHVYSFRSYRQVGILKPHPAFTHLLETAAEPTLTFETTEVPMLCPPLPWTSLH-SGAYLLSS--TKLMRATEGTTQHQRLLEQCPPAQLHGPLDALTQLGNCAWRVNGHLLDLVLQIFRDKGCMPLGVPPPRSEAPRPARYQLPPGSTPVHKSELR--KELARCLKVAREMHSLRSEALYRLSLAQHLRHRVFWLPHNMDFRGRTYPCPPHFNHLGSDLARALLEFAEGRPLGPRGLDWLKIHLINLTGLKKGDSLRMRLAFADEVMEEILDSADNPLTGRKWWMEADEPWQTLACCMEVAHAVR----SPDPAAYISHLPVHQ 892          
BLAST of DNA-directed RNA polymerase vs. UniProt/SwissProt
Match: sp|O00411|RPOM_HUMAN (DNA-directed RNA polymerase, mitochondrial OS=Homo sapiens OX=9606 GN=POLRMT PE=1 SV=2)

HSP 1 Score: 507.679 bits (1306), Expect = 2.264e-158
Identity = 336/1014 (33.14%), Postives = 534/1014 (52.66%), Query Frame = 1
Query:  583 TYHSVDKKSIIVDSVFYEPIMKLLAKNGKIDAIITLLNEMDEFHILPNISIIFRTIEAASKNELLNTEFLHRMMGYVKCHNISLEKAILKAPITKENFSHILKALQMIEPNFNIICNKPELKNKSALKETC-----------IFSSIDELEKFFEVQLNNEILTSVNVDPIHNVTVNPEILK----KVILIEDTWRSSLTEAYEKKITLLE---YSDRSKLYPYLSVLPTKSYVDAMIQVVFQLSTDSELQFNRIQHVHLHIGNIISNMFRHRCLKKQKYFAQLKSICLSYYGKL------SENPQNIEMDKLWESSIMENIDSGSIVHDTIDWSKKIKASIGKILYEIMFENMNFTFY---NGITAVTTPVIYEKLE-EDAGKVFITLKIQSKLRTIFKAINKALPGFSQLDMPMTHPPVPFHKNHESGFFYLTNYATRIIRKSTIFDIFDDDLEQ--PDDISKVYNALNVLSLCSWKIENANLDLVMQVFNDSGNMKLKIPTSANKFNGPEKPAVDLP------IKDKLNSWKSYFQFVKEQKEMYSLWSTLSYKIMIAQKLKNKPFWYPYSMDFRGRVYPCSPHLSHIGDDVSRGLLRFSLGKKLGPKGLDWIKIHLINLCGTHKRKSNSDRLKAANEVISEVIDSAANPFDGRCWWQLSEKPWQTLATCREIHKILKFVKSNGKPDDFHSTLPIHQDGSCNGLQHYXXXXXXXXXXXXVNLIQSETPQDVYSDVLEHLEVKRKEDAKNGVEEAILLEGLLSRKVIKXXXXXXXXXXXXXXXXLQILRELKNLEKLPPQNIKSCAAYLSKLTLGSIGEIFASATNIQNWFIELSSNISRLRKANISWKTPMNFIVKQPYFAD-----------------DSDI-LKSDCLKNKNAFPPNFIHSLDSTHMMLTALSCWEKNITFVSVHDCFWTHACDVDEMNKICREEFVKLHSQPILENLSDYLLEKYQFQINPNENDNGRKIAFNKLLKNIPKKGTFDIREVLDSTYFFS 3462
             +H   +K  ++    Y  +M   A+ G    ++ +L  + +  + P++      ++   + +  +   + R +  +    + L+       +++E+ + +LKA+  ++P F++    P   N S L               +   +  L+  FE QL+ E+ + V V  +   T+  + +K     +  + D W  +L  A  +    LE   Y  R  LYP+L +L  +  V  ++QV+  L    E  F  +         + +  F    +++Q+   Q++++   Y   L      +E P+     + WE      + +   + +   W   ++  +GK+L E++ +             ++   PV+Y      +  ++ I     + ++ + KA    L  F  +D+PM  PP+P+   H SG F L+   T+++R         + LE   P  +    +AL  L  C+W++    LDLV+Q+F   G  +L +P   ++   P+ P   LP       K +L    ++ Q V   +EM+SL +   Y++ +AQ L+++ FW P++MDFRGR YPC PH +H+G DV+R LL F+ G+ LGP GLDW+KIHL+NL G  KR+    RL  A EV+ +++DSA  P  GR WW  +E+PWQTLA C E+   ++       P  + S LP+HQDGSCNGLQHYAALG+D+ GAA+VNL  S+ PQDVYS V   +EV R++DA+ G+  A +LEG ++RKV+KQTVMT VYGVT+YGG LQI + L+ L   P + +   + YL +    S+ E+F+    IQ+W  E +  IS +  + + W TP+   V QPY  D                 + DI  K +  K KN FPPNFIHSLDS+HMMLTAL C+ K +TFVSVHDC+WTHA DV  MN++CRE+FV+LHS+PIL++LS +L+++  F   P +     ++   + L+ +PK G FD+ +V  STYFFS
Sbjct:  248 VHHGQRQKRKLLTLDMYNAVMLGWARQGAFKELVYVLFMVKDAGLTPDLLSYAAALQCMGRQDQ-DAGTIERCLEQMSQEGLKLQALFTAVLLSEEDRATVLKAVHKVKPTFSLPPQLPPPVNTSKLLRDVYAKDGRVSYPKLHLPLKTLQCLFEKQLHMELASRVCVVSVEKPTLPSKEVKHARKTLKTLRDQWEKALCRALRETKNRLEREVYEGRFSLYPFLCLLDEREVVRMLLQVLQALPAQGE-SFTTLAR------ELSARTFSRHVVQRQRVSGQVQALQNHYRKYLCLLASDAEVPEPCLPRQYWE-----ELGAPEALREQ-PWPLPVQMELGKLLAEMLVQATQMPCSLDKPHRSSRLVPVLYHVYSFRNVQQIGILKPHPAYVQLLEKAAEPTL-TFEAVDVPMLCPPLPWTSPH-SGAFLLS--PTKLMRTVEGATQHQELLETCPPTALHGALDALTQLGNCAWRVNGRVLDLVLQLFQAKGCPQLGVPAPPSE--APQPPEAHLPHSAAPARKAELRRELAHCQKVA--REMHSLRAEALYRLSLAQHLRDRVFWLPHNMDFRGRTYPCPPHFNHLGSDVARALLEFAQGRPLGPHGLDWLKIHLVNLTGLKKREPLRKRLAFAEEVMDDILDSADQPLTGRKWWMGAEEPWQTLACCMEVANAVR----ASDPAAYVSHLPVHQDGSCNGLQHYAALGRDSVGAASVNLEPSDVPQDVYSGVAAQVEVFRRQDAQRGMRVAQVLEGFITRKVVKQTVMTVVYGVTRYGGRLQIEKRLRELSDFPQEFVWEASHYLVRQVFKSLQEMFSGTRAIQHWLTESARLISHM-GSVVEWVTPLGVPVIQPYRLDSKVKQIGGGIQSITYTHNGDISRKPNTRKQKNGFPPNFIHSLDSSHMMLTALHCYRKGLTFVSVHDCYWTHAADVSVMNQVCREQFVRLHSEPILQDLSRFLVKR--FCSEPQKILEASQL--KETLQAVPKPGAFDLEQVKRSTYFFS 1230          
BLAST of DNA-directed RNA polymerase vs. UniProt/SwissProt
Match: sp|Q8BKF1|RPOM_MOUSE (DNA-directed RNA polymerase, mitochondrial OS=Mus musculus OX=10090 GN=Polrmt PE=1 SV=1)

HSP 1 Score: 499.204 bits (1284), Expect = 2.507e-155
Identity = 340/1013 (33.56%), Postives = 528/1013 (52.12%), Query Frame = 1
Query:  583 TYHSVDKKSIIVDSVFYEPIMKLLAKNGKIDAIITLLNEMDEFHILPNISIIFRTIEAASKNELLNTEFLHRMMGYVKCHNISLEKAILKAPITKENFSHILKALQMIEPNFNIICNKPELKNKSALKETCIFS------------SIDELEKFFEVQLNNEILTSVNVDPIHNVTVNP----EILKKVILIEDTWRSSLTEAY-EKKITLLE--YSDRSKLYPYLSVLPTKSYVDAMIQVVFQLSTDSELQFNRIQHVHLHIGNIISNMFRHRCLKKQ--KYFAQLKSICLSYYGKLSENPQNIEM--DKLWESSIMENIDSGSIVHDTIDWSKKIKASIGKILYEIMFENM----NFTFYNGITAVTTPVIYEKLEEDAGKVFITLKIQSKLRTIFKAINKALPGFSQLDMPMTHPPVPFHKNHESGFFYLTNYATRIIRKSTIFDIFDDDLEQ--PDDISKVYNALNVLSLCSWKIENANLDLVMQVFNDSGNMKLKIPTSANKFNGPEK---PAVDLPI-KDKLNSWKSYFQFVKEQKEMYSLWSTLSYKIMIAQKLKNKPFWYPYSMDFRGRVYPCSPHLSHIGDDVSRGLLRFSLGKKLGPKGLDWIKIHLINLCGTHKRKSNSDRLKAANEVISEVIDSAANPFDGRCWWQLSEKPWQTLATCREIHKILKFVKSNGKPDDFHSTLPIHQDGSCNGLQHYXXXXXXXXXXXXVNLIQSETPQDVYSDVLEHLEVKRKEDAKNGVEEAILLEGLLSRKVIKXXXXXXXXXXXXXXXXLQILRELKNLEKLPPQNIKSCAAYLSKLTLGSIGEIFASATNIQNWFIELSSNISRLRKANISWKTPMNFIVKQPYFADDS-----------------DILKSDCLKNKNAFPPNFIHSLDSTHMMLTALSCWEKNITFVSVHDCFWTHACDVDEMNKICREEFVKLHSQPILENLSDYLLEKYQFQINPNENDNGRKIAFNKL---LKNIPKKGTFDIREVLDSTYFFS 3462
            T+H+   +  ++    Y  +M   A+ G    ++ +   + +  + P++      ++   + +  +   + R +  +       +       + +E+ + +L+A+   EP F      P   N S L +  I+S             +D L+  F  QL+ E+ +SV V  +    V      E  K +  + + W   L     E K T+    Y  +  LYP+L +L    +V  ++QV+  L    E       ++ L + N      RH   +KQ   +  +L      Y   L+ + Q       + WES  +  +++ +       WS  +   +GK L E++ + +    +     G    + PV+Y      + +    LK       + +   +    F   ++PM  PP+P+   H SG + L++  T+++R +         LEQ  P  +    +AL  L  C+W++    LDLV+Q+F D G M L +P   ++   P +   P    P+ K +L   K   + +K  +EM+SL S   Y++ +AQ L+++ FW P++MDFRGR YPC PH +H+G D++R LL F+ G+ LGP+GLDW+KIHLINL G  K  S   RL  A+EV+ E++DSA NP  GR WW  +++PWQTLA C E+   ++    +  P  + S LP+HQDGSCNGLQHYAALG+D+ GAA+VNL  S+ PQDVY +V   +E  R++DAK G+  A +LEG +SRKV+KQTVMT VYGVT+YGG LQI + L+ L   P + +   + YL +    S+ E+F S   IQ+W  E S+N+       + W TP+   + QPY  +                   +  K + LK KN FPPNFIHSLDS+HMMLTAL C+ K + FVSVHDCFWTHA D+  MN++CRE+FV+LHSQPILE+L+ +L +++    +     +  +    KL   L+++PK GTFD+ +V+ STYFFS
Sbjct:  220 THHNNGDRQQVLTLHMYNTVMLGWARKGSFRELVYVFLMLKDAGLSPDLCSYAAALQCMGRRDQ-DVRTIQRCLKQMMEEGFQPQLLFTDLVLEEEDRAALLRAVVKAEPAFRPPPQAPSPVNTSTLLKD-IYSKEGPVSYPKLHLPLDTLQDLFYQQLHVELSSSVCVQSVEKAPVMSKEVIEARKTLQALREQWEVELLRVLRETKATMGRQAYEGQPTLYPFLCLLSEGEFVSILMQVLKVLPAQGEPLIQLAHNLGLRVLN------RHLVKQKQVTNHVQKLGQRYSQYLQLLASDTQVAPCLPREYWES--LGPLEAPA----QQPWSVPVLLQLGKQLAELLVQAVQMPRSLAARQGAQR-SIPVLYHVYSFRSYRQVGILKPHPAFTHLLETAAEPTLTFETTEVPMLCPPLPWTSLH-SGAYLLSS--TKLMRATEGTTQHQRLLEQCPPAQLHGPLDALTQLGNCAWRVNGHLLDLVLQIFRDKGCMPLGVPPPRSEAPRPARYQLPPGSTPVHKSELR--KELARCLKVAREMHSLRSEALYRLSLAQHLRHRVFWLPHNMDFRGRTYPCPPHFNHLGSDLARALLEFAEGRPLGPRGLDWLKIHLINLTGLKKGDSLRMRLAFADEVMEEILDSADNPLTGRKWWMEADEPWQTLACCMEVAHAVR----SPDPAAYISHLPVHQDGSCNGLQHYAALGRDSVGAASVNLTPSDLPQDVYREVATQVEEFRQQDAKEGLRVAQVLEGFISRKVVKQTVMTVVYGVTRYGGRLQIEKRLRELSDFPQEFVWEASHYLVRQVFKSLQEMFTSTRAIQHWLTE-SANLISHAGWPVEWVTPLGIPIIQPYHRESKVQVKGGLQSITLTSSVDESQKPNTLKQKNGFPPNFIHSLDSSHMMLTALHCYRKGLIFVSVHDCFWTHAADIPTMNEVCREQFVRLHSQPILEDLAKFLKKRFCSVSSIKSLKSSERALVTKLQETLQSLPKTGTFDLGQVIRSTYFFS 1207          
BLAST of DNA-directed RNA polymerase vs. UniProt/SwissProt
Match: sp|O24600|RPOT3_ARATH (DNA-directed RNA polymerase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RPOT3 PE=2 SV=1)

HSP 1 Score: 352.443 bits (903), Expect = 4.698e-103
Identity = 228/599 (38.06%), Postives = 336/599 (56.09%), Query Frame = 1
Query: 1738 MPMTHPPVPFHKNHESGFFYLTNYATRIIRKSTIFDIFDDDLEQPDDISKVYNALNVLSLCSWKIENANLDLVMQVFNDSGNMKLKIPTSANKFNGPEKPAVDLPIKDKLNSWK-SYFQFVKEQKEMYSLWSTLSYKIMIAQKLKNKP-FWYPYSMDFRGRVYPCSPHLSHIGDDVSRGLLRFSLGKKLGPKGLDWIKIHLINL-CGTHKRKSNSDRLKAANEVISEVIDSAANPFDGRCWWQLSEKPWQTLATCREIHKILKFVKSNGKPDDFHSTLPIHQDGSCNGLQHYXXXXXXXXXXXXVNLIQSETPQDVYSDVLEHLEVKRKEDAKNGVEE------AILLEGLLSRKVIKXXXXXXXXXXXXXXXXLQILRELKNLEKLPPQNIKSCAA-YLSKLTLGSIGEIFASATNIQNWFIELSSNISRLRKANISWKTPMNFIVKQPYFADDSDILKS--------------DCLKNKNAFPPNFIHSLDSTHMMLTALSCWEKNITFVSVHDCFWTHACDVDEMNKICREEFVKLHSQPILENLSDYLLEKYQFQINPNENDNGRKIAFNKLLKNIPKKGTFDIREVLDSTYFFS 3462
            +PM  PP  +    + G+ +L +Y  R        D   D   +     +V+ AL+ L    W++    LD+V +++ D GN+   +  +      PEKP+ + P  ++L SWK S  +  K  +E +SL   +  K+ +A+K+K++  F+YP+++DFRGR YP  PHL+H+  D+ RG L F+ G+ LG  GL W+KIHL NL  G  ++ S+  RL      + +++DSA NP  G+ WW  +E P+Q LA C  + + LK    +  P    S LPIHQDGSCNGLQHYAALG+D+  AAAVNL+  E P DVYS++   +    K+D+    E       A +L   + RK++KQTVMT+VYGVT  G   QI R L+    +  + +   AA Y +K+TL ++GEIF +A  I +W  + +  I+      + W TP+   V QPY   +  ++++              D  K + AFPPNF+HSLD THMM+TA++C E  + F  VHD +WTHACDVD MN+I RE+FV+L++ PILE+L     E Y                 N +   +PK+G FD++EVL S YFF+
Sbjct:  422 VPMLVPPKRWKGYDKGGYLFLPSYIMRTHGSKKQQDALKDISHK--TAHRVFEALDTLGNTKWRVNRNILDVVERLWADGGNIAGLV--NREDVPIPEKPSSEDP--EELQSWKWSARKANKINRERHSLRCDVELKLSVARKMKDEEGFYYPHNLDFRGRAYPMHPHLNHLSSDLCRGTLEFAEGRPLGKSGLHWLKIHLANLYAGGVEKLSHDARLAFVENHLDDIMDSAENPIHGKRWWLKAEDPFQCLAACVILTQALK----SPSPYSVISHLPIHQDGSCNGLQHYAALGRDSFEAAAVNLVAGEKPADVYSEISRRVHEIMKKDSSKDPESNPTAALAKILITQVDRKLVKQTVMTSVYGVTYVGAREQIKRRLEEKGVITDERMLFAAACYSAKVTLAALGEIFEAARAIMSWLGDCAKIIAS-DNHPVRWITPLGLPVVQPYCRSERHLIRTSLQVLALQREGNTVDVRKQRTAFPPNFVHSLDGTHMMMTAVACREAGLNFAGVHDSYWTHACDVDTMNRILREKFVELYNTPILEDLLQSFQESYP----------------NLVFPPVPKRGDFDLKEVLKSQYFFN 993          
BLAST of DNA-directed RNA polymerase vs. UniProt/SwissProt
Match: sp|Q8L6J3|RPO2B_TOBAC (DNA-directed RNA polymerase 2B, chloroplastic/mitochondrial OS=Nicotiana tabacum OX=4097 GN=RPOT2-TOM PE=2 SV=2)

HSP 1 Score: 340.117 bits (871), Expect = 2.310e-98
Identity = 263/800 (32.88%), Postives = 406/800 (50.75%), Query Frame = 1
Query: 1198 YLSVLPTKSY-VDAMIQVVFQLSTDSELQFNRIQHVHLHIGNIISNMFR-HRCLKKQKYFAQLKSICLSYYGKLSENPQNI--EMDKLWE--SSIMENID---SGSIVH---DTIDWSKKIKASIGKILYEIMFENMNFTFYNGITAVTTPVIYEKLEEDAGKVFITLKIQSKLRTI-------FKAINKALPGFSQLDMPMTHPPVPFHKNHESGFFYLTNYATRIIRKSTIFDIFDDDLEQPDDISKVYNALNVLSLCSWKIENANLDLVMQVFNDSGNMKLKIPTSANKFNGPEKPAV-DLPIKDKLNSWKSYFQFVK-EQKEMYSLWSTLSYKIMIAQKLKNKP-FWYPYSMDFRGRVYPCSPHLSHIGDDVSRGLLRFSLGKKLGPKGLDWIKIHLINL-CGTHKRKSNSDRLKAANEVISEVIDSAANPFDGRCWWQLSEKPWQTLATCREIHKILKFVKSNGKPDDFHSTLPIHQDGSCNGLQHYXXXXXXXXXXXXVNLIQSETPQDVYSDVLEH-LEVKRKEDAKNGVE--EAILLEGLLS---RKVIKXXXXXXXXXXXXXXXXLQILRELKNLEKLPPQN-IKSCAAYLSKLTLGSIGEIFASATNIQNWFIELSSNISRLRKANISWKTPMNFIVKQPYFADDSDILKSD--------------CLKNKNAFPPNFIHSLDSTHMMLTALSCWEKNITFVSVHDCFWTHACDVDEMNKICREEFVKLHSQPILENLSDYLLEKYQFQINPNENDNGRKIAFNKLL-KNIPKKGTFDIREVLDSTYFFS 3462
            YL  LP     V  M +++  L T  +    R+    L IG+ I    R H  L+K K     K        K  E+ +++  E +KL +  +++M+       G IV    D+  W +  KA +G  L E++ +           AV  P I          V    K  S+   I       FK + +         MPM  PPV +    + G  YL +Y  R        +         + +  V+ AL+ L    W+I    L ++ +++  +G  +L      +    PE+P   D  ++ K   W+   + VK E +E +S    +  K+ +A+K+K++  F+YP+++DFRGR YP  PHL+H+G D+ RG+L F+ G+ LG  GL W+KIHL NL  G  ++ S   R+      + ++ DSA  P +GR WW  +E P+Q LA C  + + ++    +  P+   S +P+HQDGSCNGLQHYAALG+D  GAAAVNL+  E P DVYS +    L++ +++  ++  E  +A+    L++   RK++KQTVMT+VYGVT  G   QI R LK    +   + +   A Y +K+TL ++GE+F +A +I  W  E +  I+      + W TP+   V QPY      ++K+                 + + AFPPNFIHSLD +HMM+TA++C    + F  VHD +WTHACDVD++N+I RE+FV+L+  PILE     LLE +Q              ++  LL   +P++G FD+R+VL+S YFF+
Sbjct:  258 YLYQLPADMMAVITMHKLMGLLMTGGDHGTARVVQAALVIGDAIEQEVRIHNFLEKTKKQKAEKD-------KQKEDGEHVTQEQEKLRKKVTNLMKKQKLRAVGQIVRRQDDSKPWGQDAKAKVGSRLIELLLQTAYIQPPANQLAVDPPDIRPAFLHSVRTVAKETKSASRRYGIIQCDELVFKGLERTARHMVIPYMPMLVPPVKWTGYDKGGHLYLPSYVMRTHGARQQREAVKR--ASRNQLQPVFEALDTLGSTKWRINKRVLSVIDRIW--AGGGRLADLVDRDDAPLPEEPDTEDEALRTK---WRWKVKSVKKENRERHSQRCDIELKLAVARKMKDEEGFFYPHNVDFRGRAYPMHPHLNHLGSDICRGVLVFAEGRPLGESGLRWLKIHLANLFAGGVEKLSLEGRIAFTENHMDDIFDSADKPLEGRRWWLNAEDPFQCLAVCINLSEAVR----SSSPETSISHIPVHQDGSCNGLQHYAALGRDELGAAAVNLVAGEKPADVYSGIAARVLDIMKRDAQRDPAEFPDAVRARALVNQVDRKLVKQTVMTSVYGVTYIGARDQIKRRLKERGAIADDSELFGAACYAAKVTLTALGEMFEAARSIMTWLGECAKIIAS-ENEPVRWTTPLGLPVVQPYRKIGRHLIKTSLQILTLQQETEKVMVKRQRTAFPPNFIHSLDGSHMMMTAVACRRAGLNFAGVHDSYWTHACDVDKLNRILREKFVELYETPILEK----LLESFQ-------------TSYPTLLFPPLPERGDFDLRDVLESPYFFN 1021          
BLAST of DNA-directed RNA polymerase vs. UniProt/SwissProt
Match: sp|Q8VWF8|RPOT2_NICSY (DNA-directed RNA polymerase 2, chloroplastic/mitochondrial OS=Nicotiana sylvestris OX=4096 GN=RPOT2 PE=2 SV=2)

HSP 1 Score: 337.806 bits (865), Expect = 1.355e-97
Identity = 238/696 (34.20%), Postives = 359/696 (51.58%), Query Frame = 1
Query: 1483 GSIVH---DTIDWSKKIKASIGKILYEIMFENMNFTFYNGITAVTTPVIYEKLEEDAGKVFITLKIQSKLRTI-------FKAINKALPGFSQLDMPMTHPPVPFHKNHESGFFYLTNYATRIIRKSTIFDIFDDDLEQPDDISKVYNALNVLSLCSWKIENANLDLVMQVFNDSGNMKLKIPTSANKFNGPEKPAV-DLPIKDKLNSWKSYFQFVK-EQKEMYSLWSTLSYKIMIAQKLKNK-PFWYPYSMDFRGRVYPCSPHLSHIGDDVSRGLLRFSLGKKLGPKGLDWIKIHLINL-CGTHKRKSNSDRLKAANEVISEVIDSAANPFDGRCWWQLSEKPWQTLATCREIHKILKFVKSNGKPDDFHSTLPIHQDGSCNGLQHYXXXXXXXXXXXXVNLIQSETPQDVYSDVLEHLEVKRKEDAKNGVEE------AILLEGLLSRKVIKXXXXXXXXXXXXXXXXLQILRELKNLEKLPPQN-IKSCAAYLSKLTLGSIGEIFASATNIQNWFIELSSNISRLRKANISWKTPMNFIVKQPYFADDSDILKSD--------------CLKNKNAFPPNFIHSLDSTHMMLTALSCWEKNITFVSVHDCFWTHACDVDEMNKICREEFVKLHSQPILENLSDYLLEKYQFQINPNENDNGRKIAFNKLL-KNIPKKGTFDIREVLDSTYFFS 3462
            G IV    D+  W +  +A +G  L +++ +           AV  P I          V    K  S+   I       FK + +         MPM  PPV +    + G  YL +Y  R        +         + +  V+ AL+ L    W+I    L +V +++  +G  +L      +    PE+P   D  ++ K   W+   + VK E +E +S    +  K+ +A+K+K++  F+YP+++DFRGR YP  PHL+H+G D+ RG+L F+ G+ LG  GL W+KIHL NL  G  ++ S   R+      + ++ DS+  P +GR WW  +E P+Q LA C  + + ++    +  P+   S +P+HQDGSCNGLQHYAALG+D  GAAAVNL+  E P DVYS +   +    K DA+    E      A +L   + RK++KQTVMT+VYGVT  G   QI R LK    +   + +   A Y +K+TL ++GE+F +A +I  W  E +  I+      + W TP+   V QPY      ++K+                 + + AFPPNFIHSLD +HMM+TA++C    + F  VHD +WTHACDVD++N+I RE+FV+L+  PILE     LLE +Q              ++  LL   +P++G FD+R+VL+S YFF+
Sbjct:  354 GQIVRRQDDSKPWGQDARAKVGSRLIDLLLQTAYIQPPANQLAVDPPDIRPAFVHSVRTVAKETKSASRRYGIIQCDELVFKGLERTARHMVIPYMPMLVPPVKWTGYDKGGHLYLPSYVMRTHGARQQREAVKR--ASRNQLQPVFEALDTLGNTKWRINKRVLSVVDRIW--AGGGRLADLVDRDDAPLPEEPDTEDEALRTK---WRWKVKSVKKENRERHSQRCDIELKLAVARKMKDEESFFYPHNVDFRGRAYPMHPHLNHLGSDICRGVLEFAEGRPLGESGLRWLKIHLANLFAGGVEKLSLEGRIGFTENHMDDIFDSSDKPLEGRRWWLNAEDPFQCLAVCINLSEAVR----SSSPETSVSHIPVHQDGSCNGLQHYAALGRDKLGAAAVNLVAGEKPADVYSGIAARVLDIMKRDAQRDPAEFPDAVRARVLVNQVDRKLVKQTVMTSVYGVTYIGARDQIKRRLKERGAIADDSELFGAACYAAKVTLTALGEMFEAARSIMTWLGECAKIIAS-ENEPVRWTTPLGLPVVQPYRKIGRHLIKTSLQILTLQRETEKVMVKRQRTAFPPNFIHSLDGSHMMMTAVACRRAGLNFAGVHDSYWTHACDVDKLNRILREKFVELYEAPILEK----LLESFQ-------------TSYPTLLFPPLPERGDFDMRDVLESPYFFN 1020          
BLAST of DNA-directed RNA polymerase vs. TrEMBL
Match: A0A087VZB8 (DNA-directed RNA polymerase OS=Echinococcus multilocularis OX=6211 GN=EmuJ_000150100 PE=3 SV=1)

HSP 1 Score: 605.134 bits (1559), Expect = 0.000e+0
Identity = 415/1236 (33.58%), Postives = 620/1236 (50.16%), Query Frame = 1
Query:  244 VPNLFQKPTEEISKPIDMKYV---NIIEEINQLKTEEFLPKKLEEAINITSLKLLKFFPHYK------HQKVKELNNVSALRFIKSLVYNTNNHATYMDVCIQKGDYEKALSLFNSVRNENETYHSVDKKSIIVDSVFYEPIMKLLAKNGKIDAIITLLNEMDEFHILPNISIIFRTIEA---------------ASKNELLNTEFLH----------RMMGYVKCHNISLEKAILKAPITKENFSHILKALQMI----EPNFNIICNKPELKNKSALKETC--------------------IFSSIDELEKFFEVQLNNEILTSVNVDPI-----HNVTVNPEILKKVI--------------------LIEDT-WRSSLTEAYEKKITLLEYS-DRSKL--YPYLSVLPTKSYVDAMIQVVFQLSTDSELQFNRIQHVHLHIGNIISNMFRHRCLKKQKYFA----QLKSICLSYYGKLSENPQNIE-MDKLWESSIMENIDSG-SIVHDTIDWSKKIKASIGKILYEIMFENMNFTF-------YNGITAVTTPVIYEKLEEDAGKVFITLKIQSKLRTIFKAINKALP-GFSQLDMPMTHPPVPFHKNHESGFFYLTNYATRIIRKSTIFDIFD----DDLE-QPDDISKVYNALNVLSLCSWKIENANLDLVMQVFNDSGNMKLKIPTSANKFN-GPEKPAVDLPIKDKLNSWKSYFQFVKEQKEMYSLWSTLSYKIMIAQKLKNKPFWYPYSMDFRGRVYPCSPHLSHIGDDVSRGLLRFSLGKKLGPKGLDWIKIHLINLCGTHKRKSNSDRLKAANEVISEVIDSAANPFDGRCWWQLSEKPWQTLATCREIHKILKFVKSNGKPDDFHSTLPIHQDGSCNGLQHYXXXXXXXXXXXXVNLIQSETPQDVYSDVLEHLEVKRKEDAKNGVEEAILLEGLLSRKVIKXXXXXXXXXXXXXXXXLQILRELKNLE--KLPP-----QNIKSCAAYLSKLTLGSIGEIFASATNIQNWFIELSSNISRLRKANISWKTPMNFIVKQPYF-------------------------------------------ADDS--DILKSDCLKNKNAFPPNFIHSLDSTHMMLTALSCWEKNITFVSVHDCFWTHACDVDEMNKICREEFVKLHSQPILENLSDYLLEKYQFQINP----NENDNGRKIAFNKLLKNIPKKGTFDIREVLDSTYFFS 3462
            +P+   K   E S+    KYV   +++E+IN +      P +L   +   S   L F    K          KELN++     +  + +N ++ A Y D C+Q  +  + L    + R E+    +++K ++I+    Y  I +  A      A+   L  + E  I P  +I   ++E                 S N  L TE  H           ++     + I++ + +L+ P   ++   IL AL  +    EP+   +  +         +ET                     +F S   + + F+ Q++ EI+  V V P+       +   PEI K  I                      ++T WR  LTEA+E  +  L+ +  R ++  YP+L +LP KSYVD MIQ +  + TD+ LQ        L +G  +       C+  +K  A    ++     +Y    + + +N+E   ++W  ++  N +SG S+  +   W+  I  ++G  LY I+++N+ F          + +      V++E   ++ G+    +++   L   + A +++ P  F+  ++PM  PP+P+  +  +G+   ++ ATR IRK   F   D    DDL+     I  V+++LN LS C WK+    LD ++QV    G+  L IP + + F   P K    L  ++++ +++      K   E  SLW+T  Y++ IA   ++K FW+P+SMDFRGRVYPC PH  H+G DV+RGL+ F+ G  LG +GL W+KIHL NL G  KR SN +R    + ++ E++DSA  PFDGR WW+  E+PWQTLA CRE+   L+   S   P  + S  P+HQDGSCNGLQHYAA+G+D +GAA+V+L   E P+DVYSDV E +E +R+ DA  G E AI LEG + R+VIKQ+VMTTVYGVT YG T QI R+L+ L   + P      + +   +AYL++LTL SIG IF+S+T  Q WF +L+ +I+R R   ISW TP+   V QPY                                            A DS   +   D +K +NAFPPNFIHSLDS HMMLTAL C    + F SVHDCFWTHA  VD++N+ICREEFV LHS+ +LENLSDYL EK+++         E      + FN LLK  PK+G FD+ +VL STYFFS
Sbjct:    1 MPDAMVKDVPESSQRNRSKYVTASSLLEQINAVPRARLFPSQLRLILKYQSFLSLNFLESCKTVVSEADTDCKELNSI-----LGRIYFNNDSFAAYTDACLQTAEKFQMLRSLINRRKESRIEENLEKGNVILSDSLYSLICRYFASRDDWLALERSLTWLREDGIPPTFNICVASLECLGGLLHRSIHGKSLPKSLNHKLTTEHRHIHSYVEEKALEIIALADKYGINVYEDLLRYPRQTQSLEKILTALFFVTPDREPSLTFLAAQSTTLRTYKTRETTKRVSSVLDKTPLSPANIFADVFPSSQAMVEAFQEQMHLEIMGQVPVSPVLPFFEEMLKQAPEIAKTAIHSSHQLRHRFRSAELRQSLEAEQNTHWRRQLTEAFEATLQRLKVAHARGQITAYPFLKILPKKSYVDLMIQAINTIVTDTALQHVSRSLFLLRLGERVEAA----CVVWRKQNAGILEEMAKAYKTYADMFTSSTRNVEHFREMWFRALQVNAESGASLDQEWPKWNSHILLTVGHELYRILYDNLTFNINALRSRDASKVGRQEANVLFEVSSDNPGEARYEIRVHPTLLKWYSASDRSSPLMFNPTELPMLCPPLPWIDSKHAGYLLSSSAATRFIRKPNYFPGGDASANDDLDFDISTIPSVFDSLNALSACPWKVNKPILDAMLQVARGGGDKSLSIPETKSLFPIPPRKFDSTLTREERIAAYRQAHNIRKVHDETRSLWATEMYRLSIANHYRDKVFWFPHSMDFRGRVYPCPPHFHHMGSDVARGLIVFAKGLPLGERGLRWLKIHLANLTGNVKRSSNDEREAYTDSILDEIVDSADRPFDGRGWWREQEEPWQTLACCRELTAALRHPTS---PSTYVSYFPVHQDGSCNGLQHYAAMGRDERGAASVSLEDCERPRDVYSDVTEVVEARRRADAAEGNEAAIALEGAVQRRVIKQSVMTTVYGVTLYGATAQIRRQLRELPIFQTPAGADADRRLGPASAYLARLTLSSIGAIFSSSTATQAWFAKLARHITRCRGCRISWITPLGLPVVQPYVKQANFELNLWSGELTNAAASTTAWATALTKAARCAEQQFRRGGARDSLVGLPLPDPVKQQNAFPPNFIHSLDSCHMMLTALHCLRAGVMFASVHDCFWTHAASVDQLNRICREEFVALHSENVLENLSDYLREKFEYSQAELEKLPEKKRDEAVNFNNLLKQFPKRGNFDLSKVLTSTYFFS 1224          
BLAST of DNA-directed RNA polymerase vs. TrEMBL
Match: A0A3Q0KPI7 (DNA-directed RNA polymerase OS=Schistosoma mansoni OX=6183 PE=3 SV=1)

HSP 1 Score: 585.489 bits (1508), Expect = 0.000e+0
Identity = 392/1133 (34.60%), Postives = 583/1133 (51.46%), Query Frame = 1
Query:  499 ATYMDVCIQKGDYEKALSLFNSVRNENETYHSVDKKSIIVDSVFYEPIMKLLAKNGKIDAIITLLNEMDEFHILPNISIIFRTIE--------------------AASKNELLN-------------TEFLHRMMGYVKCHNISLEKAILKAPITKENFSHILKALQMIEPNFNIICN---------------------KPELKNKSALKE------TCIFSSIDELEKFFEVQLNNEILTSVNVDPI-------HNVTVNPEILKKV--ILIEDT--WRSSLTEAYE---KKITLLEYSDRSKLYPYLSVLPTKSYVDAMIQVVFQLSTDSELQFNRIQHVHLHIGNIISNMFRHRCLKKQKYFAQLKSICLSYYGKLSENPQNIE-MDKLWESSIMENIDSG-SIVHDTIDWSKKIKASIGKILYEIMFENMNFTFYNGITAVT------------------TPVIYEKLEEDAGKVFITLKIQSKLRTIFKAINKALPGFSQLDMPMTHPPVPFHKNHESGFFYLTNYATRIIR-------KSTIFDIFDDDLEQPDDISKVYNALNVLSLCSWKIENANLDLVMQVFNDSGNMKLKIPTSANKFNGPEKPAVDL----PIKDKLNSWKSYFQFVKEQKEMYSLWSTLSYKIMIAQKLKNKPFWYPYSMDFRGRVYPCSPHLSHIGDDVSRGLLRFSLGKKLGPKGLDWIKIHLINLCGTHKRKSNSDRLKAANEVISEVIDSAANPFDGRCWWQLSEKPWQTLATCREIHKILKFVKSNGKPDDFHSTLPIHQDGSCNGLQHYXXXXXXXXXXXXVNLIQSETPQDVYSDVLEHLEVKRKEDAKNGVEEAILLEGLLSRKVIKXXXXXXXXXXXXXXXXLQILRELKNLEKLPPQNIKSCAAYLSKLTLGSIGEIFASATNIQNWFIELSSNISRLRKANISWKTPMNFIVKQPYF--------ADDS----------------DILKSDCLKNK-----------NAFPPNFIHSLDSTHMMLTALSCWEKNITFVSVHDCFWTHACDVDEMNKICREEFVKLHSQPILENLSDYLLEKYQF---QINPNENDNGRK--IAFNKLLKNIPKKGTFDIREVLDSTYFFS 3462
            A ++D  I  G+    L   +SV  E++T     +  II  S+ YE +    AK G     I LL  +    +LP+ +  F   E                    + SKN+  N                +  ++   +     L + +L   + + +  +IL AL  + PN N   N                      P  K+  ++K         IF+   ++ K F+ Q++ E +T V V P+           + PE+ K +   LI++   W   L  A+    K++ ++    +   YP+L +LP  +Y D MI  +  L  DS LQ+       L +G  +         +K  YF QL+ + ++Y    +E          +W  ++   +D G +  H+   W   I   IG++LY +++E++ F   NG   V                   TP I+E L E   +V   +K+   L   ++  +     F    +PM  PPVP+    ++G+    NYATR+IR        ST  D  D D +Q   I  V +ALN L+ C WKI N  LD ++QV    GN  L IP   +K   P++  VDL      K++  +++ Y    ++  E  SLWST  Y++ IA + ++K  W+P+SMDFRGRVYPC PH +H G DV+R L+ F+ G  LGP GLDW+KIHL+NL G  KR ++S+RL+ A+ +++++IDSA  PFDGR WWQ   +PWQ LA C E+   L        P+ + S  P+HQDGSCNGLQHYAALG+D +GA +VNL     PQD+YSDV+E +E +R+ DA  G   A  LEG + RKVIKQ++MTTVYGVT YG T QI ++L++L   P +++   + YL++LTL SI ++F S+ +IQNW  +++  IS   +  +SW+TP+   V QPY         +DD                 D++K D + +K           +AFPPNFIHSLDS HMMLT L C ++ I F SVHDCFWTHA  VD++N+ICREEF+ LHSQPIL + +DY+ +++ +   QIN  +N   R+  +AFN+LL N+PK+G+FD+ EV+ + YFFS
Sbjct:  142 AGFLDSSIASGEVFNFLRFCDSVFKESQTAKGFRRGHIIQTSMVYEKLANYYAKCGDWSGYIKLLERIKREGLLPSKNTYFYGFECLGKLMNKDGSQFPPSSHPQSISKNKAFNRLTPKSVIPMEKIGHEVENLIDAAEEEGHDLTQGLLGLSLEEGSLKNILTALLYVRPNINPNLNYFMHYSSSNISTHTKRTKEISHPAQKHLDSVKHERKNPYAGIFTDFGDISKAFKSQMHVEKMTEVTVSPVLSPFGQSETAPLTPELKKNLHNALIKERKHWHQVLLTAFNEQLKRLKIIHAKGKLTAYPFLKILPASNYADLMITGIDMLIVDSALQYTSSSIACLQLGRRVERACTVYRQEKIGYFKQLEKMYMTYATLFNEREMKFSNFRHMWLYALQSRMDQGPNTQHNWTSWPPTILFMIGEVLYSLIYEHLRFDL-NGPLRVRHEFSKSDEKASSNVRRKDTPAIFEVLAEFPTEVTNEIKVHPTLINWYRDADYPPLSFHPCALPMLCPPVPWTNLDDAGYLISRNYATRLIRCTSVSSTNSTYHDDLDFDFKQ---IPSVLDALNCLASCPWKINNTILDYLIQVARSGGNENLSIPEWRSK---PKR--VDLRNNMTAKERRLAYRKYHSDRQDYGEFCSLWSTELYRLSIANQYRDKIIWFPHSMDFRGRVYPCPPHFNHTGSDVARSLIVFAKGLPLGPNGLDWLKIHLVNLTGLKKRCTHSERLEYADSIMNDIIDSAEKPFDGRRWWQNQAEPWQILACCMELKNALSHPSG---PESYVSHFPVHQDGSCNGLQHYAALGRDLRGAKSVNLTSMNCPQDLYSDVVEIVEEQRRIDADGGNSIAKTLEGFVRRKVIKQSIMTTVYGVTSYGATEQIRKQLRDLPDFPKESLLPASRYLARLTLNSIEKVFTSSVDIQNWLCKIAKYISGDFQHRVSWQTPLGLPVIQPYIQSNKSSTDSDDYTVNFYSQLIDNNQKQIDLIKKDKIGDKLISLPKSSKQTSAFPPNFIHSLDSCHMMLTGLQCLKEGIVFASVHDCFWTHAATVDKLNRICREEFIALHSQPILSDFADYVKQRFGYTDKQINEFKNGKKREQALAFNRLLSNLPKRGSFDLSEVMKAEYFFS 1262          
BLAST of DNA-directed RNA polymerase vs. TrEMBL
Match: A0A0X3P3U9 (DNA-directed RNA polymerase (Fragment) OS=Schistocephalus solidus OX=70667 GN=RPOM PE=3 SV=1)

HSP 1 Score: 585.104 bits (1507), Expect = 0.000e+0
Identity = 395/1211 (32.62%), Postives = 616/1211 (50.87%), Query Frame = 1
Query:  283 KPIDMKYVN---IIEEINQLKTEEFLPKKLEEAINITSLKLLKFFPH---YKHQKVKELNNVSALRFIKSLVYNTNNHATYMDVCIQKGDYEKALSLFNSVRNENETYHSVDKKSIIVDSVFYEPIMKLLAKNGKIDAIITLLNEMDEFHILPNISIIFRTIEAASK--------------NELLNTEFLHR----MMGYVKCHNISLEKAILKAPITKENFSHILKALQMIEPN-FNIICNKP--------------------ELKNKSALKETC---IFSSIDELEKFFEVQLNNEILTSVNVDPIHNV------------------TVNP----EILKKVILIEDTWRSSLTEAYEKKITLLEYS---DRSKLYPYLSVLPTKSYVDAMIQVVFQLSTDSELQFNRIQHVHLHIGNIISNMFRHRCLKKQKYFAQLKSICLSY--YGKLSENPQN--IEMDKLWESSIMENIDSGSIVHDTI-DWSKKIKASIGKILYEIMFENMNFTFYNGITAVTT--------------PVIYEKLEEDAGKVFITLKIQSKLRTIFKAINKALPGFSQLDMPMTHPPVPFHKNHESGFFYLTNYATRIIRKSTIFDIFD----DDLEQPDDISKVY---NALNVLSLCSWKIENANLDLVMQVFNDSGNMKLKIP-TSANKFNGPEKPAVDLPIKDKLNSWKSYFQFVKEQKEMYSLWSTLSYKIMIAQKLKNKPFWYPYSMDFRGRVYPCSPHLSHIGDDVSRGLLRFSLGKKLGPKGLDWIKIHLINLCGTHKRKSNSDRLKAANEVISEVIDSAANPFDGRCWWQLSEKPWQTLATCREIHKILKFVKSNGK---PDDFHSTLPIHQDGSCNGLQHYXXXXXXXXXXXXVNLIQSETPQDVYSDVLEHLEVKRKEDAKNGVEEAILLEGLLSRKVIKXXXXXXXXXXXXXXXXLQILRELKNLEKLPPQN-IKSCAAYLSKLTLGSIGEIFASATNIQNWFIELSSNISRLRKANISWKTPMNFIVKQPY--------------------------------FADDSDILKS-----------DCLKNKNAFPPNFIHSLDSTHMMLTALSCWEKNITFVSVHDCFWTHACDVDEMNKICREEFVKLHSQPILENLSDYLLEKYQFQINP----NENDNGRKIAFNKLLKNIPKKGTFDIREVLDSTYFFS 3462
            +P D++ ++   ++ EIN +      P + +      S  + +F        H   K+LN + A  ++K+     ++ A Y+D C+Q  +  +AL   NS R E+    +V + S +   + +E + +  A      +I  +L  + +  + P+ + +  ++E                   + L   +L R    ++   + +NISL + +L  P  K +  +IL AL  ++P  +  +  +P                     LK+ +  KE     +F+    +   F+ QL  E+  +V+V P+ +                   +V+P     I K ++L +  W   L +A+E  +  L+ +    +   YP+L ++P ++YVD +I+ V  + TDSELQ      + + +G  +        + +++    +  + L+Y  Y  L  +P+     + + W  ++    + G  ++     W+  I+  +G+ LY I+++++ F   NG+    +              PV++E   ++  +    +++   L   ++ +      F   ++PM  PP+P+   + +G+   ++ ATR+IR+   F   D    DDL+   D SKV    +ALN L+ C WK+ N  LD ++QV    G+ +L IP T +      ++ A DLP +++  +++  +   K   E  SLW+T  Y++ IA + ++K FW+P+SMDFRGRVYPC PH  H+G DV+RGL+ F+ G  LG  GL W+K HL NL G  KR +N +R+   ++ + EV DSA  PFDGR WW+  E+PWQTLA CRE+   L+          P D+ S  P+HQDGSCNGLQHYAA+G+D +GA +V+L   E P+DVY DV E +E +R  DA  G+  A  LEG + RKVIKQ+VMTTVYGVT YG   QI R+LK + + P +  +   +AYL++LTL SI  IF S++  Q WF  LS +I++     ISW TP+   V QPY                                 A+ S   K            + LK KNAFPPNFIHSLDS HMMLTAL C+ + I F SVHDCFWTHA  VD MN+ICREEFV LHS+P+LENLSDYL  K+++         E        FNKLL+  PK+G FDI++VL STYFFS
Sbjct:   58 RPQDVQDISPCLLLSEINAIPDVRLFPSQKKLVSKFKSFLVNRFVSESVETTHDNYKDLNEILAKIYLKN-----DSLAAYVDACLQTAEVNQALKSLNSRREESRLIENVFRGSFVQADIIFELLCRHFALKSDWLSIEKVLLWLKDDGLTPSFTTLLASLECLGALLQGTRQGKKINHAGDQLLQPYLERKITEVLSLAEDNNISLYEGLLSYPRMKNSLKNILTALLYVQPGRYPHLSFQPFRPSTITDAQSQKITDRVSATLKSSTVSKENLYADVFADEKAMMNAFQAQLKLEMAGTVSVAPVLSFFEDMCKTPKGTIAATAQKSVSPVKARAIHKNLMLEQSIWSKQLMKAFEATLQRLKSAHAHGQITAYPFLKIMPRRAYVDLLIRTVNSIITDSELQHVSRSLLLVRLGERVEAAC---AVWRKQNLGIVDELKLTYEVYAGLFTSPERKAASVREAWLRALQVRSEDGICLNPQWPQWNSHIRLMVGQELYRILYDHLTFNL-NGVRLSASLPSDKEVLPPRQEAPVLFEVSSDNPAETRCEIRVHPTLLKWYRLVGHMPLQFEVTELPMLCPPIPWIDTNRAGYLLASSDATRLIRRPNYFYGTDAAANDDLDF--DASKVLLVLDALNALASCPWKVNNPILDALIQVARSGGDARLSIPETKSLPPLKSKRIARDLPREERAKAYRESYNIRKTHDETRSLWATEMYRLSIANQYRDKVFWFPHSMDFRGRVYPCPPHFHHMGSDVARGLIVFAKGLPLGENGLRWLKTHLANLSGRVKRSANFERVAFTDKYLDEVFDSADKPFDGRGWWREQEEPWQTLACCRELASALRHRNPRTGELDPADYVSFFPVHQDGSCNGLQHYAAMGRDERGAVSVSLRDCERPRDVYGDVTEVVESQRAVDAAAGMPVAQALEGFVQRKVIKQSVMTTVYGVTLYGAMSQIRRQLKEIPEFPRERWLGPASAYLARLTLASISAIFTSSSETQAWFGRLSRHITKDLSCRISWVTPLGLPVVQPYVKSAHRGLEVDFWSGSSQRQLLDYSALTRAAALAEKSRTWKGAKDSLVSLPAPNPLKQKNAFPPNFIHSLDSCHMMLTALHCFREGILFASVHDCFWTHAASVDRMNRICREEFVALHSEPLLENLSDYLRSKFEYSQTELSRLPEEKRASAEDFNKLLRQFPKRGNFDIKDVLTSTYFFS 1257          
BLAST of DNA-directed RNA polymerase vs. TrEMBL
Match: A0A068WX42 (DNA-directed RNA polymerase OS=Echinococcus granulosus OX=6210 GN=EgrG_000150100 PE=3 SV=1)

HSP 1 Score: 583.178 bits (1502), Expect = 0.000e+0
Identity = 401/1223 (32.79%), Postives = 608/1223 (49.71%), Query Frame = 1
Query:  244 VPNLFQKPTEEISKPIDMKYV---NIIEEINQLKTEEFLPKKLEEAINITSLKLLKFFPHYK------HQKVKELNNVSALRFIKSLVYNTNNHATYMDVCIQKGDYEKALSLFNSVRNENETYHSVDKKSIIVDSVFYEPIMKLLAKNGKIDAIITLLNEMDEFHILPNISIIFRTIEA---------------ASKNELLNTEFLH----------RMMGYVKCHNISLEKAILKAPITKENFSHILKALQMI----EPNFNIICNKPELKNKSALKETC--------------------IFSSIDELEKFFEVQLNNEILTSVNVDPI-----HNVTVNPEILKKVI--------------------LIEDT-WRSSLTEAYEKKITLLEYS-DRSKL--YPYLSVLPTKSYVDAMIQVVFQLSTDSELQFNRIQHVHLHIGNIISNMFRHRCLKKQKYFA----QLKSICLSYYGKLSENPQNIE-MDKLWESSIMENIDSG-SIVHDTIDWSKKIKASIGKILYEIMFENMNFTF-------YNGITAVTTPVIYEKLEEDAGKVFITLKIQSKLRTIFKAINKALP-GFSQLDMPMTHPPVPFHKNHESGFFYLTNYATRIIRKSTIFDIFD----DDLE-QPDDISKVYNALNVLSLCSWKIENANLDLVMQVFNDSGNMKLKIPTSANKFN-GPEKPAVDLPIKDKLNSWKSYFQFVKEQKEMYSLWSTLSYKIMIAQKLKNKPFWYPYSMDFRGRVYPCSPHLSHIGDDVSRGLLRFSLGKKLGPKGLDWIKIHLINLCGTHKRKSNSDRLKAANEVISEVIDSAANPFDGRCWWQLSEKPWQTLATCREIHKILKFVKSNGKPDDFHSTLPIHQDGSCNGLQHYXXXXXXXXXXXXVNLIQSETPQDVYSDVLEHLEVKRKEDAKNGVEEAILLEGLLSRKVIKXXXXXXXXXXXXXXXXLQILRELKNLEKL-------PPQNIKSCAAYLSKLTLGSIGEIFASATNIQNWFIELSSNISRLRKANISWKTPMNFIVKQPYF-------------------------------------------ADDS--DILKSDCLKNKNAFPPNFIHSLDSTHMMLTALSCWEKNITFVSVHDCFWTHACDVDEMNKICREEFVKLHSQPILENLSDYLLEKYQFQINP----NENDNGRKIAFNKLLKNIPKKGTF 3423
            +P+   K   E  +    KYV   +++E+IN +      P +L   +   S   L F    K          KEL+++     +  + +N ++ A Y D C+Q  +  + L    + R E+    +++K ++I+    YE I +  A      A+   L  + E  I P  +I   ++E                 S N  L TE  H           ++   + + I++ + +L+ P   ++   IL AL  +    EP+ + +  +         +ET                     +F S   + + F+ Q++ EI+  V V P+       +   PEI K  I                      ++T WR  LTEA+E  +  L+ +  R ++  YP+L +LP KSYVD MIQ +  + TD+ LQ        L +G  +       C+  +K  A    ++     +Y    + + +N+E   ++W  ++  N +SG S+  +   W+  I  ++G  LY I+++N+ F          + +      V++E   ++ G+    +++   L   + A +++ P  F+  ++PM  PP+P+     +G+   ++ ATR IRK   F   D    DDL+     I  V+++LN L+ C WK+    LD ++QV    G+  L+IP + + F   P K    L  ++++ +++      K   E  SLW+T  Y++ IA   ++K FW+P+SMDFRGRVYPC PH  H+G DV+RGL+ F+ G  LG +GL W+KIHL NL G  KR SN +R    + ++ E++DSA  PFDGR WW+  E+PWQTLA CRE+   L+   S   P  + +  P+HQDGSCNGLQHYAA+G+D +GAA+V+L   E P+DVYSDV E +E +R+ DA  G E AI LEG + R+VIKQ+VMTTVYGVT YG   QI R+L+ L            + +   +AYL++LTL SIG IF+S+T  Q WF +L+ +I+R R   ISW TP+   V QPY                                            A DS   +   D +K +NAFPPNFIHSLDS HMMLTAL C    + F SVHDCFWTHA  VD++N+ICREEFV LHS+ +LENLSDYL EK+++         E      + FN LLK  PK+G F
Sbjct:    1 MPDAMVKDVPESLQRYRSKYVTASSLLEQINAVPRARLFPSQLRLILKYQSFLSLNFLESCKTVVSEADTDCKELSSI-----LGRIYFNNDSFAAYTDACLQTAEKFQMLRSLINRRKESRIEENLEKGNVILSDSLYELICRYFASRDDWLALERSLTWLREDGIPPTFNICIASLECLGGLLHRSIHGKSLPKSLNHKLTTEHRHIHSYVEEKSLEIIALAEKYGINVYEDLLRYPRQTQSLEKILTALFFVTPDREPSLSFLAPQSTTLRTYKTRETTKRVSTVLDKTPLSPVNIFADVFPSSQAMVEAFQEQMHLEIMGQVPVSPVLPFFEEMLKEAPEIAKTAIHSSHQLRHRFRPAELRQSLEAEQNTHWRRQLTEAFEATLQRLKVAHARGQITAYPFLKILPKKSYVDLMIQAIDTIVTDTALQHVSRSLFLLRLGERVEAA----CVVWRKQNAGILEEMAKAYKTYADMFTSSTRNVEHFREMWFRALQVNAESGASLDQEWPKWNSHILLTVGHELYRILYDNLTFNINALRSRDASKVGRQEANVLFEVSSDNPGEARYEIRVHPTLLKWYSASDRSSPLMFNPTELPMLCPPLPWIDTKHAGYLLSSSAATRFIRKPNYFPGGDASANDDLDFDISTIPSVFDSLNALAACPWKVNKPILDAMLQVARGGGDKSLRIPETKSLFPIPPRKFDSTLTREERMAAYRQAHNIRKVHDETRSLWATEMYRLSIANHYRDKVFWFPHSMDFRGRVYPCPPHFHHMGSDVARGLIVFAKGLPLGERGLRWLKIHLANLTGNVKRSSNDEREAYTDSILDEIVDSADRPFDGRGWWREQEEPWQTLACCRELTAALRHPTS---PSTYVNYFPVHQDGSCNGLQHYAAMGRDERGAASVSLEDCERPRDVYSDVTEVVEARRRADAAEGNEVAIALEGAVQRRVIKQSVMTTVYGVTLYGAMAQIRRQLRELPTFQTPAGADADRRLGPASAYLARLTLSSIGAIFSSSTATQAWFAKLAKHITRCRGCRISWITPLGLPVVQPYVNQADFELNLWSGELSNAAASTTAWATALTKAARRAEQQFRRGGARDSLVGLPLPDPVKQQNAFPPNFIHSLDSCHMMLTALHCLRAGVMFASVHDCFWTHAASVDQLNRICREEFVALHSENVLENLSDYLREKFEYSQAELEKLPEKKRDEAVNFNNLLKQFPKRGKF 1211          
BLAST of DNA-directed RNA polymerase vs. TrEMBL
Match: A0A4Z2DVV8 (DNA-directed RNA polymerase OS=Schistosoma japonicum OX=6182 GN=EWB00_003926 PE=3 SV=1)

HSP 1 Score: 573.548 bits (1477), Expect = 0.000e+0
Identity = 356/907 (39.25%), Postives = 514/907 (56.67%), Query Frame = 1
Query:  979 FSSIDELEKFFEVQLNNEILTSVNVDPI-------HNVTVNPEI---LKKVILIEDT-WRSSLTEAYEKKITLLEYSD-RSKL--YPYLSVLPTKSYVDAMIQVVFQLSTDSELQFNRIQHVHLHIGNIISNMFRHRCLKKQKYFAQLKSICLSYYGKLSENPQNI-EMDKLWESSIMENIDSG-SIVHDTIDWSKKIKASIGKILYEIMFENMNF-----------------TFYNGITAVTTPVIYEKLEEDAGKVFITLKIQSKLRTIFKAINKALPGFSQLDMPMTHPPVPFHKNHESGFFYLTNYATRIIRKSTIF---DIFDDDLE-QPDDISKVYNALNVLSLCSWKIENANLDLVMQVFNDSGNMKLKIPTSANKFNGPEKPAVDLPIKDKLNSWKSYFQFVKEQKEMYSLWSTLSYKIMIAQKLKNKPFWYPYSMDFRGRVYPCSPHLSHIGDDVSRGLLRFSLGKKLGPKGLDWIKIHLINLCGTHKRKSNSDRLKAANEVISEVIDSAANPFDGRCWWQLSEKPWQTLATCREIHKILKFVKSNGKPDDFHSTLPIHQDGSCNGLQHYXXXXXXXXXXXXVNLIQSETPQDVYSDVLEHLEVKRKEDAKNGVEEAILLEGLLSRKVIKXXXXXXXXXXXXXXXXLQILRELKNLEKLPPQNIKSCAAYLSKLTLGSIGEIFASATNIQNWFIELSSNISRLRKANISWKTPMNFIVKQPYFADDSDILKS---------------------DCLKN----------------KNAFPPNFIHSLDSTHMMLTALSCWEKNITFVSVHDCFWTHACDVDEMNKICREEFVKLHSQPILENLSDYLLEKYQF---QINPNENDNGRK--IAFNKLLKNIPKKGTFDIREVLDSTYFFS 3462
            F+S D++ K F+ QL  E +  V + P+          ++ PE+   L   ++ E + WR +L  A+ +++  L+ +  R KL  YP+L +LP  +YVD MI  +  L  DS LQ+       L +G  +         +K  YF QL+ I ++Y    +E+   +     +W  S+  +ID G +  H    W   I   IG++LY +++E++ F                 T  N I    TP I+E L E   +V   +K+   L   ++        F    +PM  PP+P+    ++G+    NY+TR+IR S+I     I+ DDL+     I  V +ALN L+ C WKI N+ LD ++ V    GN  L IP   +K    E    D+  K++  ++K YF+  ++  E  SLWST  Y++ IA + ++K  W+P+SMDFRGRVYPC PH +H G DV+R L+ F+ G  LG  G DW+KIHL+NL G  KR ++S+RL+ AN +++++IDSA  PFDGR WWQ   +PWQ LA C EI   L  +   G P+ + S  P+HQDGSCNGLQHYAALG+D +GA +VNL   + PQD+YSDV+E +E +R+ DA  G   A  L+G + RKVIKQ++MTTVYGVT YG T QI R+L++L   P  ++   + YL++LTL SIG++F S+ +IQNW  E++  IS      +SW+TP+   V QPY   +   +                       D LKN                 +AFPPNFIHSLDS HM+LT L C ++ I F SVHDCFWTHA  VD++N+ICREEF+ LHSQPIL + +DY+  K+ +   +IN  +N   R+  +A N+LL ++PKKG+FD+ EV+ STYFFS
Sbjct:  106 FTSSDDISKAFKNQLYLEQMLEVPISPVLSPFGQSETTSLPPELKISLHNALVKEQSHWRKTLLIAFNEQLKRLKLTHARGKLTAYPFLKILPASNYVDLMITGIDMLIVDSALQYTSSSFACLQLGRRVERACTVYRQEKSGYFKQLERIYMTYATLFNEHEMKLLNFRHMWLYSLQSHIDQGPNTNHSWTSWPPSILFMIGEVLYNLIYEHLRFDLNGPLKVRHEFLKHDNTISNNIRRQDTPAIFEVLTEFPTEVTNEIKVHPTLINWYRDAGYPPLSFHPCALPMLCPPIPWINLMDAGYLISRNYSTRLIRSSSISSTNPIYSDDLDFDYKQIPSVLDALNCLASCPWKINNSILDYLIHVARSGGNENLSIPEWRSKPKRVEL-KTDMTRKERQLAYKKYFEDRQDYGEFCSLWSTELYRLSIANQYRDKTIWFPHSMDFRGRVYPCPPHFNHTGSDVARSLIVFAKGLPLGSNGFDWLKIHLVNLTGLKKRSTHSERLEYANSIMNDIIDSAEKPFDGRRWWQDQAEPWQILACCIEIKNAL--LHPCG-PESYVSHFPVHQDGSCNGLQHYAALGRDLRGAKSVNLTDMDRPQDLYSDVVEIVEDQRRIDADGGNTIAKALDGYVRRKVIKQSIMTTVYGVTPYGATEQIRRQLRDLPDFPKDSLLPASRYLARLTLNSIGKVFTSSVDIQNWLCEIAKYISGDLHCRVSWQTPLGLPVIQPYMESNKSYMSDPDDYAVNVYGQPNHNINQAQLDLLKNYKQNVNSISLPKSSKQTSAFPPNFIHSLDSCHMILTGLQCLKEGIMFASVHDCFWTHAATVDKLNRICREEFIALHSQPILGDFADYVKNKFAYTEKEINEFKNPKKREQALALNRLLSSLPKKGSFDLSEVMKSTYFFS 1008          
BLAST of DNA-directed RNA polymerase vs. Ensembl Cavefish
Match: polrmt (RNA polymerase mitochondrial [Source:NCBI gene;Acc:103025872])

HSP 1 Score: 520.39 bits (1339), Expect = 1.758e-163
Identity = 363/1047 (34.67%), Postives = 562/1047 (53.68%), Query Frame = 1
Query:  505 YMDVCIQKGDYEKALSLFNSVRNENETYHSVDKKSIIVDSVFYEPIMKLLAKNGKIDAIITLLNEMDEFHILPNISIIFRTIEAASKNELLNTEFLHRMMGYVKCHNISLEKAILKAPITKENFSHILKALQMIEPNF-------NIICNKPELKNKSALKETCIFSSID----ELEKFFEVQLNNEILTSVNVD---PIHNVTVNPEILKKVILIE-DTWRSSLTEAYEKKITLLEYSD----RSKLYPYLSVLPTKSYVDAMIQVVFQLSTDSELQFNRIQHVHLHIGNIISNMFRHRCLKKQ-KYFAQLKSICLSYYGKLSENPQ--NIEMDKLWESSIMENIDSGSIVHDTIDWSKKIKASIGKILYEIMFENMNFT---FYNGITAVTTPVIYE----KLEEDAGKVF---ITLKIQSKLRTIFKAINKALPGFSQLDMPMTHPPVPFHKNHESGFFYLTNYATRIIRKSTIFDIFDDDLE--QPDDISKVYNALNVLSLCSWKIENANLDLVMQVFNDSGNMKLKIPTSANKFNGPEKPAVD-----LPIKDKLNSWKSYFQFVKEQKEMYSLWSTLSYKIMIAQKLKNKPFWYPYSMDFRGRVYPCSPHLSHIGDDVSRGLLRFSLGKKLGPKGLDWIKIHLINLCGTHKRKSNSDRLKAANEVISEVIDSAANPFDGRCWWQLSEKPWQTLATCREIHKILKFVKSNGKPD--DFHSTLPIHQDGSCNGLQHYXXXXXXXXXXXXVNLIQSETPQDVYSDVLEHLEVKRKEDAKNGVEEAILLEGLLSRKVIKXXXXXXXXXXXXXXXXLQILRELKNLEKLPPQNIKSCAAYLSKLTLGSIGEIFASATNIQNWFIELSSNISRLRKANISWKTPMNFIVKQPYFADDSDILKS-----------------DCLKNKNAFPPNFIHSLDSTHMMLTALSCWEKNITFVSVHDCFWTHACDVDEMNKICREEFVKLHSQPILENLSDYLLEKYQFQINPNENDNGRKIAFNK---LLKNIPKKGTFDIREVLDSTYFFS 3462
            Y++ C+  GD E+A   F  + + N     + K+S++    FY  +MK+ AK G +  I  L   + E  + P+       +E   +    +T  + R +  +K   + ++    +     +    +LKA++  +P++       +  C+   +K+  A +E   F  +D    EL++ F+ QL  E   ++ +D    I  VT N   ++K++ ++   W+ SL +A  +   +L+       ++ LYP L VL  + YV  M+Q +  L    +   N  +     +G+ + + F   C++++ +   ++  I  SY   L+++ +  N+   + W     E     S++     W   +   +G  L  ++   +          +     P++Y     +     G +    I +KIQS   T+ K        F    MPM  PP+P+  + +SG + LT   T+++R     +     LE  Q +D+  V ++LN L  C+WKI    LD+++ +FND G  KL IP    +   PE P  +       I++K +  +   +  K+  EM+SL     YK+ IA  ++++ FW+P++MDFRGR YPC P+L+H+G D++R +L F+ GK LGP GLDW+KIHL+NL G  KR S + RL+ AN ++ +++DSA NP  GR WW  +++PWQ LA C+EI        ++  PD  +F S  P+HQDGSCNGLQHYAALG+D  GA +VNL+  E PQDVYS V + +E  RK DA  G++ AI+LEG ++RKV+KQTVMT VYGVT+YGG LQI + LK ++  P + +   + YL +    S+ E+F     IQNW  E +  IS+   + + W TP+   + QPY    S +++S                 D +K KNAFPPNFIHSLDSTHMMLTAL C+   +TFVSVHDCFWTH   VD MNK+CRE+FV LH+QPIL+NLS++LL+KY          N   + FN+   LL  +P+ G FD++ V DSTYFFS
Sbjct:  273 YLEACVYTGDVERA-QRFLLLHHRN-----LSKRSVLTVR-FYNTLMKMWAKKGALPQISKLFVMLAEAELKPDAGSYSAVLECMGRMPESSTNLIERCLEQMKEDGLCVDDVFSQCLFRLDERDMVLKAVRDGKPDYEPPTSAKSPQCSLTLVKDFYAQREDVRFPKMDFELAELQERFKQQLAVEQSNTITIDSVEAIKPVTENMTKMRKLLEVQYGHWQRSLLQALRESKQVLKKGRLRNWKTDLYPLLCVLHDEEYVKIMLQTLENLPPTGDSLVNLAKE----LGSRVYSTF---CIQQKIQMVKKMSHIYNSYAELLAKDTESSNVLPRERWIELEAEMSAGPSLLSVEASWPHMLLVQLGCFLANLLVCELKIPSNILNPELDNKLIPILYHMYIFRNSRQIGFIKPHPILVKIQSDA-TVTKLT------FDSYVMPMLCPPIPW-TSPKSGAYLLT--PTKLMRAVDGANQHQLLLEKCQNEDLHAVLDSLNQLGNCAWKINKPMLDIIISIFNDKGCDKLDIPPPLTE--APEIPRFNPHDQSYTIEEKAHLKQEIMKARKKYGEMHSLRMDALYKLSIANHMRDEIFWFPHNMDFRGRTYPCPPYLNHLGSDMTRCILLFAEGKPLGPTGLDWLKIHLVNLTGLKKRSSLAGRLEYANSIMDDILDSADNPLTGRGWWMSADEPWQCLACCKEI------ASAHRSPDHTEFISCFPVHQDGSCNGLQHYAALGRDVIGATSVNLMPCEEPQDVYSGVAQQVEEFRKRDAAKGLKTAIVLEGFINRKVVKQTVMTVVYGVTRYGGRLQIEKRLKEIDDFPKEYLWDASHYLVQQVFSSLKEMFTGTREIQNWLTESARLISK-SGSTVEWVTPLGLPIVQPYHRSRSQLVRSNIQMLNVQISYDVHEKPDTMKQKNAFPPNFIHSLDSTHMMLTALYCYSAGVTFVSVHDCFWTHPITVDTMNKVCREQFVSLHNQPILQNLSEFLLQKYCS--GSVGKTNKAFLEFNRMQQLLARVPETGDFDLQRVKDSTYFFS 1284          
BLAST of DNA-directed RNA polymerase vs. Ensembl Cavefish
Match: polrmt (RNA polymerase mitochondrial [Source:NCBI gene;Acc:103025872])

HSP 1 Score: 479.174 bits (1232), Expect = 6.437e-149
Identity = 275/609 (45.16%), Postives = 380/609 (62.40%), Query Frame = 1
Query: 1723 FSQLDMPMTHPPVPFHKNHESGFFYLTNYATRIIRKSTIFDIFDDDLE--QPDDISKVYNALNVLSLCSWKIENANLDLVMQVFNDSGNMKLKIPTSANKFNGPEKPAVD-----LPIKDKLNSWKSYFQFVKEQKEMYSLWSTLSYKIMIAQKLKNKPFWYPYSMDFRGRVYPCSPHLSHIGDDVSRGLLRFSLGKKLGPKGLDWIKIHLINLCGTHKRKSNSDRLKAANEVISEVIDSAANPFDGRCWWQLSEKPWQTLATCREIHKILKFVKSNGKPD--DFHSTLPIHQDGSCNGLQHYXXXXXXXXXXXXVNLIQSETPQDVYSDVLEHLEVKRKEDAKNGVEEAILLEGLLSRKVIKXXXXXXXXXXXXXXXXLQILRELKNLEKLPPQNIKSCAAYLSKLTLGSIGEIFASATNIQNWFIELSSNISRLRKANISWKTPMNFIVKQPYFADDSDILKS-----------------DCLKNKNAFPPNFIHSLDSTHMMLTALSCWEKNITFVSVHDCFWTHACDVDEMNKICREEFVKLHSQPILENLSDYLLEKYQFQINPNENDNGRKIAFNK---LLKNIPKKGTFDIREVLDSTYFFS 3462
            F    MPM  PP+P+  + +SG + LT   T+++R     +     LE  Q +D+  V ++LN L  C+WKI    LD+++ +FND G  KL IP    +   PE P  +       I++K +  +   +  K+  EM+SL     YK+ IA  ++++ FW+P++MDFRGR YPC P+L+H+G D++R +L F+ GK LGP GLDW+KIHL+NL G  KR S + RL+ AN ++ +++DSA NP  GR WW  +++PWQ LA C+EI        ++  PD  +F S  P+HQDGSCNGLQHYAALG+D  GA +VNL+  E PQDVYS V + +E  RK DA  G++ AI+LEG ++RKV+KQTVMT VYGVT+YGG LQI + LK ++  P + +   + YL +    S+ E+F     IQNW  E +  IS+   + + W TP+   + QPY    S +++S                 D +K KNAFPPNFIHSLDSTHMMLTAL C+   +TFVSVHDCFWTH   VD MNK+CRE+FV LH+QPIL+NLS++LL+KY          N   + FN+   LL  +P+ G FD++ V DSTYFFS
Sbjct:  610 FDSYVMPMLCPPIPW-TSPKSGAYLLT--PTKLMRAVDGANQHQLLLEKCQNEDLHAVLDSLNQLGNCAWKINKPMLDIIISIFNDKGCDKLDIPPPLTE--APEIPRFNPHDQSYTIEEKAHLKQEIMKARKKYGEMHSLRMDALYKLSIANHMRDEIFWFPHNMDFRGRTYPCPPYLNHLGSDMTRCILLFAEGKPLGPTGLDWLKIHLVNLTGLKKRSSLAGRLEYANSIMDDILDSADNPLTGRGWWMSADEPWQCLACCKEI------ASAHRSPDHTEFISCFPVHQDGSCNGLQHYAALGRDVIGATSVNLMPCEEPQDVYSGVAQQVEEFRKRDAAKGLKTAIVLEGFINRKVVKQTVMTVVYGVTRYGGRLQIEKRLKEIDDFPKEYLWDASHYLVQQVFSSLKEMFTGTREIQNWLTESARLISK-SGSTVEWVTPLGLPIVQPYHRSRSQLVRSNIQMLNVQISYDVHEKPDTMKQKNAFPPNFIHSLDSTHMMLTALYCYSAGVTFVSVHDCFWTHPITVDTMNKVCREQFVSLHNQPILQNLSEFLLQKYCS--GSVGKTNKAFLEFNRMQQLLARVPETGDFDLQRVKDSTYFFS 1204          

HSP 2 Score: 63.1586 bits (152), Expect = 1.986e-9
Identity = 62/268 (23.13%), Postives = 123/268 (45.90%), Query Frame = 1
Query:  505 YMDVCIQKGDYEKALSLFNSVRNENETYHSVDKKSIIVDSVFYEPIMKLLAKNGKIDAIITLLNEMDEFHILPNISIIFRTIEAASKNELLNTEFLHRMMGYVKCHNISLEKAILKAPITKENFSHILKALQMIEPNF-------NIICNKPELKNKSALKETCIFSSID----ELEKFFEVQLNNEILTSVNVD---PIHNVTVNPEILKKVILIE-DTWRSSLTEAYEKKITLLEYSD----RSKLYPYLSVLPTKSYVDAMIQVV 1251
            Y++ C+  GD E+A   F  + + N     + K+S++    FY  +MK+ AK G +  I  L   + E  + P+       +E   +    +T  + R +  +K   + ++    +     +    +LKA++  +P++       +  C+   +K+  A +E   F  +D    EL++ F+ QL  E   ++ +D    I  VT N   ++K++ ++   W+ SL +A  +   +L+       ++ LYP L VL  + YV  M+Q +
Sbjct:  273 YLEACVYTGDVERA-QRFLLLHHRN-----LSKRSVLT-VRFYNTLMKMWAKKGALPQISKLFVMLAEAELKPDAGSYSAVLECMGRMPESSTNLIERCLEQMKEDGLCVDDVFSQCLFRLDERDMVLKAVRDGKPDYEPPTSAKSPQCSLTLVKDFYAQREDVRFPKMDFELAELQERFKQQLAVEQSNTITIDSVEAIKPVTENMTKMRKLLEVQYGHWQRSLLQALRESKQVLKKGRLRNWKTDLYPLLCVLHDEEYVKIMLQTL 533          
BLAST of DNA-directed RNA polymerase vs. Ensembl Sea Lamprey
Match: ENSPMAT00000005603.1 (pep scaffold:Pmarinus_7.0:GL476532:352361:404828:1 gene:ENSPMAG00000005070.1 transcript:ENSPMAT00000005603.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 362.844 bits (930), Expect = 1.295e-109
Identity = 272/896 (30.36%), Postives = 452/896 (50.45%), Query Frame = 1
Query:  505 YMDVCIQKGDYEKALSLFNSVRNENETYHSVDKKSIIVDSVFYEPIMKLLAKNGKIDAIITLLNEMDEFHILPNISIIFRTIEAASKNELLNTEFLHRMMGYVKCHNISLEKAILKAPITKENFSHILKALQMIEPNFNIICNKPELKNKS------ALKETC--------IFSSIDELEKFFEVQLNNE---ILTSVNVDPIHNVTVNPEILKK---VILIEDTWRSSLTEAY---EKKITLLEYSDRSKL--YPYLSVLPTKSYVDAMIQVVFQL--STDSELQFNRIQHVHLHIGNIISNMFRHRCLKK--QKYFAQLKSICLSYYGKLSENPQNIEM---DKLWESSIMENIDSGSIVHDTIDWSKKIKASIGKILYEIMFENMNFTFYNGITAVTT-----PVIYEKLEEDAGKVFITLK---IQSKLRTIFKAINKALPGFSQLDMPMTHPPVPFHKNHESGFFYLTNYATRIIRKSTIFDIFDDDLEQPDDISKVYNALNVLSLCSWKIENANLDLVMQVFNDSGNMKLKIPTSANKFNGPEKPAVDLP----IKDKLNSWKSYFQFVKEQKEMYSLWSTLSYKIMIAQKLKNKPFWYPYSMDFRGRVYPCSPHLSHIGDDVSRGLLRFSLGKKLGPKGLDWIKIHLINLCGTHKRKSNSDRLKAANEVISEVIDSAANPFDGRCWWQLSEKPWQTLATCREIHKILKFVKSNGKPDDFHSTLPIHQDGSCNGLQHYXXXXXXXXXXXXVNLIQSETPQDVYSDVLEHLEVKRKEDAKNGVEEAILLEGLLSRKVIKXXXXXXXXXXXXXXXXLQILRELKNLEKLPPQNIKSCAAYLSKLTLGSIGEIFASATNIQNWFIELSSNISRLRKANISWKTPMNFIVKQPY 3060
            Y+D C+  G+ ++A       +     YH       ++    Y+ +M   AK   +  I  L + + E  + P++      +E   +   +    + R +  ++ + + L++        ++    +L A++ + P++  +   P L          AL   C        +  + +EL++ F  Q++ E   I+T  +V+ +  VT  PE+ KK   +  +   WR++L +A+   +KK+    +    KL  YPY  +L  + YV+A    +  L  + DS +  +R      ++G  + NM+   C++K  + + AQ  S     Y +L  +   +      + WE    E  D   +  +T++W+  +  ++G  L +IM   +      G T +       P +Y      + +    +K   + ++L++  +A+   L  F    +P + PP+P+      G+  +     R +  +T      D  ++   +    + LN L  CSW I    LD+V+ +FND G  KL IP   ++   P+ P  D      I D+L   ++     K   EM+SL  +  YK+ IA  ++ + FW+P++MDFRGR YPC PH +H+G D+SR LL F+ G+ LG +GLDWIKIHL+NL GT K+ S  +R   A+E + +++DSA  P  GR WW  +++PWQ LA C EI + ++    +  P ++ S  P HQDGSCNGLQHYAALG+D  GAA+VNL   + PQDVY+ V + ++V R EDAKN V+ A +L G + RK++KQTVMT VYGVT+YGG  QI + L  ++  P + +   +AYL+KL   S+ E+F     IQNW  E +  I+R  + ++ W TP+   + QPY
Sbjct:    8 YLDACLFSGELKRA-------QKYVWHYHQDPNLRSLLSVQIYDVMMGAWAKKKFLFKIAKLFSMVGEAGLKPSLVSYAAALECMGRVPNIAQSVVARCLEEMQQNGLELDQLFAHCNFVEDEREVVLAAVRRVRPDYAPMAMPPRLVAPLSVVPLLALSYPCDNSGNYPRLKQTTEELKQGFRRQMDLEKAHIVTIQSVEALKPVT--PEMEKKRQALTELHKEWRATLLQAFAEGKKKLMSRRFGSSFKLDLYPYTCLLTDEEYVNAHAGGLNSLPPNGDSVMSLSR------NLGRKVFNMY---CIRKKMRNHVAQRISKVYDAYCELLASDGKVSGFLPRQFWERVEAEVADGYRLSGETVEWTLPVTVTLGSYLLDIMVRELKVYSGTGSTDLVRGTKPIPALYHVYAFRSVRQIGFVKPHPLVTQLQS--RAVETEL-HFDVQALPTSCPPLPWTSPRFGGYVLIPTTLMRSVDMATQHQNLLDLCQEARRLHAAMDVLNQLGTCSWIINEPLLDIVIGIFNDRGCDKLDIPPPLSE--APKVPRYDPTGQNGIADRLAFRRALTHARKTTSEMHSLRMSALYKLSIANAMRGRVFWFPHNMDFRGRTYPCPPHFNHLGSDMSRALLLFAEGRPLGERGLDWIKIHLVNLTGTKKKSSLLERRMFADEKMDDILDSADRPLTGRQWWMEADEPWQVLACCMEIARAVR----SPDPKEYISHFPTHQDGSCNGLQHYAALGRDTIGAASVNLKPLDMPQDVYASVAQQVDVFRAEDAKNDVKIAQVLGGFIGRKLVKQTVMTVVYGVTRYGGRQQIEKRLSEMDDFPKEYVWDASAYLTKLVFSSLREMFTGTREIQNWLTESARMIARAGQ-SVEWVTPLGLPIIQPY 875          
BLAST of DNA-directed RNA polymerase vs. Ensembl Yeast
Match: RPO41 (Mitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; requires a specificity subunit encoded by MTF1 for promoter recognition; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation; Rpo41p also synthesizes RNA primers for mitochondrial DNA replication [Source:SGD;Acc:S000001858])

HSP 1 Score: 338.576 bits (867), Expect = 7.396e-98
Identity = 253/786 (32.19%), Postives = 394/786 (50.13%), Query Frame = 1
Query: 1123 RSSLTEAYEKKITLLEY-SDRSKLYPYLSVL-PTKSYVDAMIQVVFQLSTDSELQFNRIQHVHLHIGNIISNMFR-HRCLKKQKYFAQLKSICLSYYGKLSENPQNIEMDKLWESSIMENIDSGSIVHDTIDWSKKIKASIGKILYEIMFENMNFTFYNGITAVT-------TPVIYEKLEEDAGKVFITLKIQSKLRTIFKAIN--KALPGFSQLDMPMTHPPVPFHKNHESGFFYLTNYATRIIRKSTIFDIFDDDLEQPDDISKVYNALNVLSLCSWKIENANLDLVMQVFNDSGNMKLKIPTSANKFNGPEKPA--VDLPIKDKLNSWKSYFQFVKEQ-KEMYSLWSTLSYKIMIAQKLKNKPFWYPYSMDFRGRVYPCSPHLSHIGDDVSRGLLRFSLGKKLGPKGLDWIKIHLINLCGTHKRKSNSDRLKAANEVISEVIDSAANPFDGRCWWQLSEKPWQTLATCREIHKILKFVKSNGKPDDFHSTLPIHQDGSCNGLQHYXXXXXXXXXXXXVNLIQSETPQDVYSDVLEHLEVKRKEDAKNGVEEAILLEGLLSRKVIKXXXXXXXXXXXXXXXXLQILRELKNLEKLPPQNIKSCAAYLSKLTLGSIGEIFASATNIQNWFIELSSNISRLRKANIS-----------------WKTPMNFIVKQPY----------------FADDSDILKSDCLKNKNAFPPNFIHSLDSTHMMLTALSCWEKNITFVSVHDCFWTHACDVDEMNKICREEFVKLHSQPILENLSDYLLEKYQ 3336
            RS L+E    K  L +  ++R    PYL+++ P K  V  +++++   ST   ++  R     + +G  I   FR  + LK +    +  +     + KL +N +++               S  I    I W + I+A IG +L  ++ +    +   G+  VT        P      +   G     LKI    +T+ + +N  + +       +PM   P P+  N  SG ++ T       + S     +        DI +VY+ LNVL    W +     D+V QV+N  G   L IP + ++   P  P    D  I   L +WK   + +  +     S     +YK+ IA+    +  ++P+++DFRGR YP SPH +H+G+D+SRGLL F  GKKLGP GL W+KIHL NL G  K     DR+      + ++ DSA NP  G  WW  ++KPWQ LATC E+++++K       P++F S  P+HQDG+CNGLQHYAALG D +GA  VNL+ S+ PQDVY+ V   ++ + +  A+ G E A +L+  ++RKV+KQTVMT VYGVT  G T QI ++L  +     +++   + YL+K    +I E+F SA  IQ+W  E +  IS+  + ++                  W TP+   + QPY                 +D   +   +  + K   PPNFIHSLD++HM+L+A  C ++ + F SVHD +WTHA D+D MN + RE+F+KLH   ++  L +   ++Y+
Sbjct:  473 RSLLSEKLSDKKGLNKVDTNRLGYGPYLTLIDPGKMCVITILELLKLNSTGGVIEGMRTARAVISVGKAIEMEFRSEQVLKSESQAFRDVNKKSPEFKKLVQNAKSV-------------FRSSQIEQSKILWPQSIRARIGSVLISMLIQVAKVSV-QGVDPVTKAKVHGEAPAFAHGYQYHNGSKLGVLKIH---KTLIRQLNGERLIASVQPQLLPMLVEPKPW-VNWRSGGYHYTQSTLLRTKDSPEQVAYLKAASDNGDIDRVYDGLNVLGRTPWTVNRKVFDVVSQVWN-KGEGFLDIPGAQDEMVLPPAPPKNSDPSI---LRAWKLQVKTIANKFSSDRSNRCDTNYKLEIARAFLGEKLYFPHNLDFRGRAYPLSPHFNHLGNDMSRGLLIFWHGKKLGPSGLKWLKIHLSNLFGFDKLPL-KDRVAFTESHLQDIKDSAENPLTGDRWWTTADKPWQALATCFELNEVMKM----DNPEEFISHQPVHQDGTCNGLQHYAALGGDVEGATQVNLVPSDKPQDVYAHVARLVQKRLEIAAEKGDENAKILKDKITRKVVKQTVMTNVYGVTYVGATFQIAKQLSPIFDDRKESL-DFSKYLTKHVFSAIRELFHSAHLIQDWLGESAKRISKSIRLDVDEKSFKNGNKPDFMSSVIWTTPLGLPIVQPYREESKKQVETNLQTVFISDPFAVNPVNARRQKAGLPPNFIHSLDASHMLLSAAECGKQGLDFASVHDSYWTHASDIDTMNVVLREQFIKLHEVDLVLRLKEEFDQRYK 1230          
BLAST of DNA-directed RNA polymerase vs. Ensembl Nematostella
Match: EDO28665 (DNA-directed RNA polymerase [Source:UniProtKB/TrEMBL;Acc:A7T5U5])

HSP 1 Score: 356.681 bits (914), Expect = 1.591e-112
Identity = 197/418 (47.13%), Postives = 267/418 (63.88%), Query Frame = 1
Query: 2131 TLSYKIMIAQKLKNKPFWYPYSMDFRGRVYPCSPHLSHIGDDVSRGLLRFSLGKKLGPKGLDWIKIHLINLCGTHKRKSNSDRLKAANEVISEVIDSAANPFDGRCWWQLSEKPWQTLATCREIHKILKFVKSNGKPDDFHSTLPIHQDGSCNGLQHYXXXXXXXXXXXXVNLIQSETPQDVYSDVLEHLEVKRKEDAKNGVEEAILLEGLLSRKVIKXXXXXXXXXXXXXXXXLQILRELKNLEKLPPQNIKSCAAYLSKLTLGSIGEIFASATNIQNWFIELSSNISRLRKANISWKTPMNFIVKQPYFADDSDILKS-----------------DCLKNKNAFPPNFIHSLDSTHMMLTALSCWEKNITFVSVHDCFWTHACDVDEMNKICREEFVKLHSQPILENLSDYLLEKY 3333
            T  YK+ +A   +++ FW P +MDFRGRVYP  PH  H+GDD+SRG+L+F+ G  LGP GL W+KIHL NL G  K+ S  +R++  ++++ EV+DSA NP  G  WW   ++PWQ LATC EI K ++    +  P  + S  P+HQDGSCNGLQHYAALG D  GA  VNLI ++ PQDVY++V + +E  R  DA+ G   A LLE  ++R+V+KQTVMT VYGVT  GG LQI R+LK+L+ L  + +   +AYL      SIG++F  A  IQ+WF   +++I+      + W TP++  V QPY       +K+                 D  K + AFPPNF+HSLDSTHMMLTA+ C    I F SVHD FWTHA  +D MN++CRE+FV LH+QPIL++L  + ++ Y
Sbjct:    2 TALYKLSVANHFRDRVFWLPLNMDFRGRVYPIPPHCCHVGDDLSRGMLQFAEGVPLGPDGLRWLKIHLANLYGAKKKSSLDERVEYTDQMMPEVLDSADNPLTGARWWMHGDEPWQVLATCMEIAKAVR----SPDPAQYVSHQPVHQDGSCNGLQHYAALGHDRYGARQVNLIPADRPQDVYAEVAKLVEEARLHDAEQGHRIATLLEDKVTRRVVKQTVMTIVYGVTFVGGRLQIERQLKDLD-LDERVLFEASAYLVHKVFASIGQMFTRAKAIQDWFALSATHIAYTGHC-VDWYTPLSLYVCQPYCKKAVKTIKTRLQGIEIPTQGFAMSVPDSRKQRAAFPPNFVHSLDSTHMMLTAVHCQRAGINFASVHDSFWTHAAHIDIMNRLCREQFVSLHTQPILQDLQAHFVDNY 413          
BLAST of DNA-directed RNA polymerase vs. Ensembl Nematostella
Match: EDO41702 (DNA-directed RNA polymerase [Source:UniProtKB/TrEMBL;Acc:A7S3D0])

HSP 1 Score: 340.887 bits (873), Expect = 1.634e-105
Identity = 210/506 (41.50%), Postives = 295/506 (58.30%), Query Frame = 1
Query: 1738 MPMTHPPVPFHKNHESGFFYLTNYATRIIRKSTIFDIFDDDLEQ----PDDISKVYNALNVLSLCSWKIENANLDLVMQVFNDSGNMKLKIPTSANKFNGPEKPAVDLPIKDKLNSWKSYFQFVKEQK-------EMYSLWSTLSYKIMIAQKLKNKPFWYPYSMDFRGRVYPCSPHLSHIGDDVSRGLLRFSLGKKLGPKGLDWIKIHLINLCGTHKRKSNSDRLKAANEVISEVIDSAANPFDGRCWWQLSEKPWQTLATCREIHKILKFVKSNGKPDDFHSTLPIHQDGSCNGLQHYXXXXXXXXXXXXVNLIQSETPQDVYSDVLEHLEVKRKEDAKNGVEEAILLEGLLSRKVIKXXXXXXXXXXXXXXXXLQILRELKNLEKLPPQNIKSCAAYLSKLTLGSIGEIFASATNIQNWFIELSSNISRLRKANISWKTPMNFIVKQPYFADDSDILKS-----------------DCLKNKNAFPPNFIHSLDSTHMMLTAL 3171
            +PM  PP P+    + GF        +++R +   D   D + +      ++S V ++LN L  C+WK  N  LDL +QVFND G+  L IP       GP  P    P++  +   ++  + ++E +       E YSL  T  YK+ +A   +++ FW P +MDFRGRVYP  PH  H+GDD+SRG+L+F+ G  LGP GL W+KIHL NL G  K+ S  +R++  ++++SEV+DSA NP  G  WW   ++PWQ LATC EI K ++    +  P  + S  P+HQDGSCNGLQHYAALG D  GA  VNLI ++ PQDVY++V + +E  R  DA+ G   A LLE  ++R+V+KQTVMT VYGVT  GG LQI R+LK+L+ L  + +   +AYL      SIG++F  A  IQ+WF   +++I+      + W TP++  V QPY       +K+                 D  K + AFPPNF+HSLDSTHMMLTA+
Sbjct:    6 LPMLVPPRPWRGIRDGGFLI---QPVQLMRSTQEMDDNHDHMLRYKCDSRELSAVLDSLNYLGTCAWKTNNRILDLAIQVFNDQGDDTLAIP-------GPVVPLELEPLEKSVKEPEARMKQIQEHRRARKLAREHYSLRMTALYKLSVANHFRDRVFWLPLNMDFRGRVYPIPPHCCHVGDDLSRGMLQFAEGVPLGPDGLRWLKIHLANLYGAKKKSSLDERVEYTDQMMSEVLDSADNPLTGARWWMHGDEPWQVLATCMEIAKAVR----SPDPAQYVSHQPVHQDGSCNGLQHYAALGHDRYGARQVNLIPADRPQDVYAEVAKLVEEARLHDAEQGHRIATLLEDKVTRRVVKQTVMTIVYGVTFVGGRLQIERQLKDLD-LDERVLFEASAYLVHKVFASIGQMFTRAKAIQDWFALSATHIAYTGHC-VDWYTPLSLYVCQPYCKKAVKTIKTRLQGIEIPTQGFAMSVPDSRKQRAAFPPNFVHSLDSTHMMLTAV 495          
BLAST of DNA-directed RNA polymerase vs. Ensembl Nematostella
Match: EDO47752 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RKW2])

HSP 1 Score: 180.259 bits (456), Expect = 4.194e-47
Identity = 113/263 (42.97%), Postives = 155/263 (58.94%), Query Frame = 1
Query: 2731 GVEEAILLEGLLSRKVIKXXXXXXXXXXXXXXXXLQILRELKNLEKLPPQNIKSCAAYLSKLTLGSIGEIFASATNIQNWFIELSSNISRLRKANISWKTPMNFIVKQPYFADDSDILKS-----------------DCLKNKNAFPPNFIHSLDSTHMMLTALSCWEKNITFVSVHDCFWTHACDVDEMNKICREEFVKLHSQPILENLSDYLLEKY---QFQINPNENDNGRKIAFNKLLKNIPKKGTFDIREVLDSTYFF 3459
            G   A LLE  ++R+V+KQTVMT VYGVT  GG LQI R+LK+L+ L  + +   +AYL      SIG++F  A  IQ+WF   +++I+      + W TP++  V QPY       +K+                 D  K + AFPPNF+HSLDSTHMMLTA+ C    I F SVHD FWTHA  +D MN++CRE+FV LH+QPIL++L  + ++ Y   +F     + D+      N   + +P+ G FD+ EVLDSTYFF
Sbjct:  427 GHRIATLLEDKVTRRVVKQTVMTIVYGVTFVGGRLQIERQLKDLD-LDERVLFEASAYLVHKVFASIGQMFTRAKAIQDWFALSATHIAYTGHC-VDWYTPLSLYVCQPYCKKAVKTIKTRLQGIEIPTQGFAMSVPDSRKQRAAFPPNFVHSLDSTHMMLTAVHCQRAGINFASVHDSFWTHAAHIDIMNRLCREQFVSLHTQPILQDLQTHFVDNYGGLKFMRPLKKKDHAP--IHNATFEGLPEIGDFDVSEVLDSTYFF 685          

HSP 2 Score: 113.62 bits (283), Expect = 1.128e-25
Identity = 78/294 (26.53%), Postives = 135/294 (45.92%), Query Frame = 1
Query: 1429 NIEMDKLWESSIMENIDSGSIVHDTIDWSKKIKASIGKILYEIMFENMNFTFYNGITAVTTPVIYEKLEEDAGKVFITLKIQSKLRTIFKAI-----NKALPGFSQLDMPMTHPPVPFHKNHESGFFYLTNYATRIIRKSTIFDIFDDDLEQ----PDDISKVYNALNVLSLCSWKIENANLDLVMQVFNDSGNMKLKIPTSANKFNGPEKPAVDLPIKDKLNSWKSYFQFVKEQK-------EMYSLWSTLSYKIMIAQKLKNKPFWYPYSMDFRGRVYPCSPHLSHIGDDVS 2262
             +++ + +++S+ + ID        + W+ K+      I + I    ++   +N  +    P  Y   E    +    +K  S +  +FK       + A+P  S   +PM  PP P+    + GF        +++R +   D   D + +      ++S V ++LN L  C+WK  N  LDL +QVFND G+  L IP       GP  P    P++  +   ++  + ++E +       E YSL  T  YK+ +A   +++ FW P +MDFRGRVYP  PH  H+G  ++
Sbjct:  158 GLDLGRPYDASLKQEIDD-------LPWATKLLCGCTLIDFLIQSVKIDANLFNAKSESLLPGFYHSYEFRERRKIGVIKPHSVVCKLFKGYLADHGDIAMPTTS---LPMLVPPRPWRDIRDGGFLI---QPVQLMRSTQEMDDNHDHMLRYKCDSRELSAVLDSLNYLGTCAWKTNNRILDLAIQVFNDQGDDTLAIP-------GPVVPLELEPLEKSVKEPEARMKQIQEHRRARKLAREHYSLRMTALYKLSVANHFRDRVFWLPLNMDFRGRVYPIPPHCCHVGHRIA 431          
BLAST of DNA-directed RNA polymerase vs. Ensembl Medaka
Match: polrmt (RNA polymerase mitochondrial [Source:NCBI gene;Acc:101162744])

HSP 1 Score: 544.658 bits (1402), Expect = 5.337e-172
Identity = 360/1038 (34.68%), Postives = 554/1038 (53.37%), Query Frame = 1
Query:  502 TYMDVCIQKGDYEKALSLFNSVRNENETYHSVDKKSIIVDSVFYEPIMKLLAKNGKIDAIITLLNEMDEFHILPNISIIFRTIEAASKNELLNTEFLHRMMGYVKCHNISLEKAILKAPITKENFSHILKALQMIEPNFNII-------CNKPELKNKSALKETCIFS----SIDELEKFFEVQLNNEILTSVNVDPIHN---VTVNPEILKKVILIEDT-WRSSLTEAYEKKITLLEYSD----RSKLYPYLSVLPTKSYVDAMIQVVFQLSTDSELQFNRIQHVHLHIGNIISNMFRHRCLKKQKYFAQLKSICLSYYGKLSENPQ--NIEMDKLWESSIMENIDSGSIVHDTIDWSKKIKASIGKILYEIMFENM--NFTFYN-GITAVTTPVIYEKLEEDAGKVFITLKIQSKLRTIFKAINKALPGFSQLDMPMTHPPVPFHKNHESGFFYLTNYATRIIRKSTIFDIFDDDLEQPDDISKVYNALNVLSLCSWKIENANLDLVMQVFNDSGNMKLKIPTSANK------FNGPEKPAVDLPIKDKLNSWKSYFQFVKEQKEMYSLWSTLSYKIMIAQKLKNKPFWYPYSMDFRGRVYPCSPHLSHIGDDVSRGLLRFSLGKKLGPKGLDWIKIHLINLCGTHKRKSNSDRLKAANEVISEVIDSAANPFDGRCWWQLSEKPWQTLATCREIHKILKFVKSNGKPDDFHSTLPIHQDGSCNGLQHYXXXXXXXXXXXXVNLIQSETPQDVYSDVLEHLEVKRKEDAKNGVEEAILLEGLLSRKVIKXXXXXXXXXXXXXXXXLQILRELKNLEKLPPQNIKSCAAYLSKLTLGSIGEIFASATNIQNWFIELSSNISRLRKANISWKTPMNFIVKQPYFADDSDILKS-----------------DCLKNKNAFPPNFIHSLDSTHMMLTALSCWEKNITFVSVHDCFWTHACDVDEMNKICREEFVKLHSQPILENLSDYLLEKYQFQIN-PNENDNGRKIAFNK---LLKNIPKKGTFDIREVLDSTYFFS 3462
            +Y++ CI  GD E+A     S        H V  +  ++++  Y  +M++ AK G ++ I  +   ++E  + PN+      +E   +N   + + + R +  ++   ++L+         ++    +L+A++ +EP +          C  P + +    +E   +     S +EL + F+ QL+ E   +V +D +     VT N   +++++  + T W   L +A  +   +L  ++    R  LYPYL +L    YVD MIQ V  +    E     ++ +   +G+ +   +  R   + +   +L SI  +Y   L+++ +   +   + W    ME     S+  D   W   +   +G  L +IM +N+  N    N        P++Y      + +    +K    L  + +   +    F    MPM  PPVP+  + + G + LT   T+++R        +  LE+   +  V+++LN L  C+WKI    LD+++ +FND G+ KL IP   ++      FN P+ PA     K  +   +      K+  EM+SL     YK+ IA  ++ + FW+P++MDFRGR YPC P+ +H+G DV+R +L F+ GK LGPKGLDW+KIHL+NL G  KR S   RL+ AN ++ +++DSA NP  GR WW  +++PWQ LA C EI +  +    +  P  F S  P+HQDGSCNGLQHYAALG+D  GA +VNL+  + PQDVYS V + +E  R  DA++G++ A +LEG +SRKV+KQTVMT VYGVT+YGG LQI + LK +E  P ++I   + YL +L    + E+F     IQ+W  E +  IS+     + W TP+   + QPY    + +LKS                 D +K KNAFPPNFIHSLDSTHMMLTAL C+   +TFVSVHDCFWTHA  VD MNK+CRE+FV LHSQPIL+ LSD+LL KY      P E  + +   + +   LL  +P+ G FD+++V +STYFFS
Sbjct:  333 SYLEACIFIGDVERAQRFLLS-------QHRVRSRRKLLNTGVYNIMMRVWAKKGMLNQIGRIFILLEEAGLKPNLGSYSAALECMGRNPNCSPKAISRCLNQMEEDGLTLDDLFSDCVFRRDEKDMMLQAIRAVEPGYEPRPHLLTDECPSPLVHHFYTQREHHSYPKLALSQEELLERFQQQLSVERACTVTIDSVETTKPVTENMRKMRELLAEQRTHWEKILHQALRESKMILANTNTRDYRLNLYPYLCLLEDGEYVDIMIQSVANMPPSGE----SLKVLARDLGHRVYTKYCVRQKFENQAVEKLGSIYSAYTELLAKDTKVSRVLPREQWTRLQMEQSSGPSLQGDDTSWPHIVTLELGTHLVDIMVKNLKINSDILNPSQDRKLIPILYHMYTFRSTRQVGFIKPHPILVQMQQEAMETRLTFDSYVMPMLCPPVPW-TSVKFGAYVLT--PTKLMRTVEGATQHEQLLEKSQHLEPVFDSLNQLGNCAWKINKPLLDIIISIFNDRGSDKLDIPPPMSEAPKIPHFN-PQDPAYTASQKALMK--REVINAKKKGSEMHSLRMDALYKLSIAHHMREETFWFPHNMDFRGRTYPCPPYFNHLGSDVTRAMLVFAEGKPLGPKGLDWLKIHLVNLTGLKKRSSLQGRLQYANSIMEDILDSADNPLHGRKWWMNADEPWQALACCMEIARAAR----SPDPAKFVSHFPVHQDGSCNGLQHYAALGRDVIGATSVNLVPCDVPQDVYSGVAQQVEELRARDARDGLKIAQVLEGFISRKVVKQTVMTVVYGVTRYGGRLQIEKRLKEIENFPKEHIWDASHYLVRLVFNGLKEMFTGTREIQDWLTESARLISKC-GHTVEWVTPLGLPIIQPYHRTRNQVLKSSIQHINLQISHDSKERPDTVKQKNAFPPNFIHSLDSTHMMLTALHCYSAGLTFVSVHDCFWTHALTVDTMNKVCREQFVALHSQPILQELSDFLLHKYCSGSGLPAEAKSKKYQEYRRLMLLLTKVPQTGDFDLQQVKESTYFFS 1348          
BLAST of DNA-directed RNA polymerase vs. Planmine SMEST
Match: SMESG000026344.1 (SMESG000026344.1)

HSP 1 Score: 2097.78 bits (5434), Expect = 0.000e+0
Identity = 1051/1056 (99.53%), Postives = 1053/1056 (99.72%), Query Frame = 1
Query:  295 MKYVNIIEEINQLKTEEFLPKKLEEAINITSLKLLKFFPHYKHQKVKELNNVSALRFIKSLVYNTNNHATYMDVCIQKGDYEKALSLFNSVRNENETYHSVDKKSIIVDSVFYEPIMKLLAKNGKIDAIITLLNEMDEFHILPNISIIFRTIEAASKNELLNTEFLHRMMGYVKCHNISLEKAILKAPITKENFSHILKALQMIEPNFNIICNKPELKNKSALKETCIFSSIDELEKFFEVQLNNEILTSVNVDPIHNVTVNPEILKKVILIEDTWRSSLTEAYEKKITLLEYSDRSKLYPYLSVLPTKSYVDAMIQVVFQLSTDSELQFNRIQHVHLHIGNIISNMFRHRCLKKQKYFAQLKSICLSYYGKLSENPQNIEMDKLWESSIMENIDSGSIVHDTIDWSKKIKASIGKILYEIMFENMNFTFYNGITAVTTPVIYEKLEEDAGKVFITLKIQSKLRTIFKAINKALPGFSQLDMPMTHPPVPFHKNHESGFFYLTNYATRIIRKSTIFDIFDDDLEQPDDISKVYNALNVLSLCSWKIENANLDLVMQVFNDSGNMKLKIPTSANKFNGPEKPAVDLPIKDKLNSWKSYFQFVKEQKEMYSLWSTLSYKIMIAQKLKNKPFWYPYSMDFRGRVYPCSPHLSHIGDDVSRGLLRFSLGKKLGPKGLDWIKIHLINLCGTHKRKSNSDRLKAANEVISEVIDSAANPFDGRCWWQLSEKPWQTLATCREIHKILKFVKSNGKPDDFHSTLPIHQDGSCNGLQHYXXXXXXXXXXXXVNLIQSETPQDVYSDVLEHLEVKRKEDAKNGVEEAILLEGLLSRKVIKXXXXXXXXXXXXXXXXLQILRELKNLEKLPPQNIKSCAAYLSKLTLGSIGEIFASATNIQNWFIELSSNISRLRKANISWKTPMNFIVKQPYFADDSDILKSDCLKNKNAFPPNFIHSLDSTHMMLTALSCWEKNITFVSVHDCFWTHACDVDEMNKICREEFVKLHSQPILENLSDYLLEKYQFQINPNENDNGRKIAFNKLLKNIPKKGTFDIREVLDSTYFFS 3462
            MKYVNIIEEINQLKTEEFLPKKLEEAINITSLKLLKFFPHYKHQKVKELNNVSALRFIKSLVYNTNNHATYMDVCIQKGDYEKALSLFNSVRNENETYHSVDKKSIIVDSVFYEPIMKLLAKNGKIDAIITLLNEMDEFHILPNISIIFRTIEAASKNELLNTEFLHRMMGYVKCHNISLEKAILKAPITKENFSHILKALQMIEPNFNIICNKPELKNKSALKETCI SSIDELEKFFEVQLNNEILTSVNVDPI NVTVNPEILKKVILIEDTWRSSLTEAYEKKITLLEYSDRSKLYPYLSVLPTKSYVDAMIQVVFQLSTDSELQFNRIQHVHLHIGNIISNMFRHRCLKKQKYFAQLKSICLSYYGKLS+NPQNIEMDKLWESSIMENIDSGSIVHDTIDWSKKIKASIGKILYEIMFENMNFTFYNGITAVTTPVIYEKLEEDAGKVFITLKIQSKLRTIFKAINKALPGFSQLDMPMTHPPVPFHKNHESGFFYLTNYATRIIRKSTIFDIFDDDLEQPDDISKVYNALNVLSLCSWKIENANLDLVMQVFNDSGNMKLKIPTSANKFNGPEKPAVDLPIKDKLNSWKSYFQFVKEQKEMYSLWSTLSYKIMIAQKLKNKPFWYPYSMDFRGRVYPCSPHLSHIGDDVSRGLLRFSLGKKLGPKGLDWIKIHLINLCGTHKRKSNSDRLKAAN+VISEVIDSA NPFDGRCWWQLSEKPWQTLATCREIHKILKFVKSNGKPDDFHSTLPIHQDGSCNGLQHYAALGKDAKGAAAVNLIQSETPQDVYSDVLEHLEVKRKEDAKNGVEEAILLEGLLSRKVIKQTVMTTVYGVTQYGGTLQILRELKNLEKLPPQNIKSCAAYLSKLTLGSIGEIFASATNIQNWFIELSSNISRLRKANISWKTPMNFIVKQPYFADDSDILKSDCLKNKNAFPPNFIHSLDSTHMMLTALSCWEKNITFVSVHDCFWTHACDVDEMNKICREEFVKLHSQPILENLSDYLLEKYQFQINPNENDNGRKIAFNKLLKNIPKKGTFDIREVLDSTYFFS
Sbjct:    1 MKYVNIIEEINQLKTEEFLPKKLEEAINITSLKLLKFFPHYKHQKVKELNNVSALRFIKSLVYNTNNHATYMDVCIQKGDYEKALSLFNSVRNENETYHSVDKKSIIVDSVFYEPIMKLLAKNGKIDAIITLLNEMDEFHILPNISIIFRTIEAASKNELLNTEFLHRMMGYVKCHNISLEKAILKAPITKENFSHILKALQMIEPNFNIICNKPELKNKSALKETCISSSIDELEKFFEVQLNNEILTSVNVDPIRNVTVNPEILKKVILIEDTWRSSLTEAYEKKITLLEYSDRSKLYPYLSVLPTKSYVDAMIQVVFQLSTDSELQFNRIQHVHLHIGNIISNMFRHRCLKKQKYFAQLKSICLSYYGKLSDNPQNIEMDKLWESSIMENIDSGSIVHDTIDWSKKIKASIGKILYEIMFENMNFTFYNGITAVTTPVIYEKLEEDAGKVFITLKIQSKLRTIFKAINKALPGFSQLDMPMTHPPVPFHKNHESGFFYLTNYATRIIRKSTIFDIFDDDLEQPDDISKVYNALNVLSLCSWKIENANLDLVMQVFNDSGNMKLKIPTSANKFNGPEKPAVDLPIKDKLNSWKSYFQFVKEQKEMYSLWSTLSYKIMIAQKLKNKPFWYPYSMDFRGRVYPCSPHLSHIGDDVSRGLLRFSLGKKLGPKGLDWIKIHLINLCGTHKRKSNSDRLKAANDVISEVIDSAVNPFDGRCWWQLSEKPWQTLATCREIHKILKFVKSNGKPDDFHSTLPIHQDGSCNGLQHYAALGKDAKGAAAVNLIQSETPQDVYSDVLEHLEVKRKEDAKNGVEEAILLEGLLSRKVIKQTVMTTVYGVTQYGGTLQILRELKNLEKLPPQNIKSCAAYLSKLTLGSIGEIFASATNIQNWFIELSSNISRLRKANISWKTPMNFIVKQPYFADDSDILKSDCLKNKNAFPPNFIHSLDSTHMMLTALSCWEKNITFVSVHDCFWTHACDVDEMNKICREEFVKLHSQPILENLSDYLLEKYQFQINPNENDNGRKIAFNKLLKNIPKKGTFDIREVLDSTYFFS 1056          
BLAST of DNA-directed RNA polymerase vs. Planmine SMEST
Match: SMESG000026344.1 (SMESG000026344.1)

HSP 1 Score: 2063.5 bits (5345), Expect = 0.000e+0
Identity = 1036/1041 (99.52%), Postives = 1038/1041 (99.71%), Query Frame = 1
Query:  295 MKYVNIIEEINQLKTEEFLPKKLEEAINITSLKLLKFFPHYKHQKVKELNNVSALRFIKSLVYNTNNHATYMDVCIQKGDYEKALSLFNSVRNENETYHSVDKKSIIVDSVFYEPIMKLLAKNGKIDAIITLLNEMDEFHILPNISIIFRTIEAASKNELLNTEFLHRMMGYVKCHNISLEKAILKAPITKENFSHILKALQMIEPNFNIICNKPELKNKSALKETCIFSSIDELEKFFEVQLNNEILTSVNVDPIHNVTVNPEILKKVILIEDTWRSSLTEAYEKKITLLEYSDRSKLYPYLSVLPTKSYVDAMIQVVFQLSTDSELQFNRIQHVHLHIGNIISNMFRHRCLKKQKYFAQLKSICLSYYGKLSENPQNIEMDKLWESSIMENIDSGSIVHDTIDWSKKIKASIGKILYEIMFENMNFTFYNGITAVTTPVIYEKLEEDAGKVFITLKIQSKLRTIFKAINKALPGFSQLDMPMTHPPVPFHKNHESGFFYLTNYATRIIRKSTIFDIFDDDLEQPDDISKVYNALNVLSLCSWKIENANLDLVMQVFNDSGNMKLKIPTSANKFNGPEKPAVDLPIKDKLNSWKSYFQFVKEQKEMYSLWSTLSYKIMIAQKLKNKPFWYPYSMDFRGRVYPCSPHLSHIGDDVSRGLLRFSLGKKLGPKGLDWIKIHLINLCGTHKRKSNSDRLKAANEVISEVIDSAANPFDGRCWWQLSEKPWQTLATCREIHKILKFVKSNGKPDDFHSTLPIHQDGSCNGLQHYXXXXXXXXXXXXVNLIQSETPQDVYSDVLEHLEVKRKEDAKNGVEEAILLEGLLSRKVIKXXXXXXXXXXXXXXXXLQILRELKNLEKLPPQNIKSCAAYLSKLTLGSIGEIFASATNIQNWFIELSSNISRLRKANISWKTPMNFIVKQPYFADDSDILKSDCLKNKNAFPPNFIHSLDSTHMMLTALSCWEKNITFVSVHDCFWTHACDVDEMNKICREEFVKLHSQPILENLSDYLLEKYQFQINPNENDNGRKIAFNKLLKNIPKKG 3417
            MKYVNIIEEINQLKTEEFLPKKLEEAINITSLKLLKFFPHYKHQKVKELNNVSALRFIKSLVYNTNNHATYMDVCIQKGDYEKALSLFNSVRNENETYHSVDKKSIIVDSVFYEPIMKLLAKNGKIDAIITLLNEMDEFHILPNISIIFRTIEAASKNELLNTEFLHRMMGYVKCHNISLEKAILKAPITKENFSHILKALQMIEPNFNIICNKPELKNKSALKETCI SSIDELEKFFEVQLNNEILTSVNVDPI NVTVNPEILKKVILIEDTWRSSLTEAYEKKITLLEYSDRSKLYPYLSVLPTKSYVDAMIQVVFQLSTDSELQFNRIQHVHLHIGNIISNMFRHRCLKKQKYFAQLKSICLSYYGKLS+NPQNIEMDKLWESSIMENIDSGSIVHDTIDWSKKIKASIGKILYEIMFENMNFTFYNGITAVTTPVIYEKLEEDAGKVFITLKIQSKLRTIFKAINKALPGFSQLDMPMTHPPVPFHKNHESGFFYLTNYATRIIRKSTIFDIFDDDLEQPDDISKVYNALNVLSLCSWKIENANLDLVMQVFNDSGNMKLKIPTSANKFNGPEKPAVDLPIKDKLNSWKSYFQFVKEQKEMYSLWSTLSYKIMIAQKLKNKPFWYPYSMDFRGRVYPCSPHLSHIGDDVSRGLLRFSLGKKLGPKGLDWIKIHLINLCGTHKRKSNSDRLKAAN+VISEVIDSA NPFDGRCWWQLSEKPWQTLATCREIHKILKFVKSNGKPDDFHSTLPIHQDGSCNGLQHYAALGKDAKGAAAVNLIQSETPQDVYSDVLEHLEVKRKEDAKNGVEEAILLEGLLSRKVIKQTVMTTVYGVTQYGGTLQILRELKNLEKLPPQNIKSCAAYLSKLTLGSIGEIFASATNIQNWFIELSSNISRLRKANISWKTPMNFIVKQPYFADDSDILKSDCLKNKNAFPPNFIHSLDSTHMMLTALSCWEKNITFVSVHDCFWTHACDVDEMNKICREEFVKLHSQPILENLSDYLLEKYQFQINPNENDNGRKIAFNKLLKNIPKKG
Sbjct:    1 MKYVNIIEEINQLKTEEFLPKKLEEAINITSLKLLKFFPHYKHQKVKELNNVSALRFIKSLVYNTNNHATYMDVCIQKGDYEKALSLFNSVRNENETYHSVDKKSIIVDSVFYEPIMKLLAKNGKIDAIITLLNEMDEFHILPNISIIFRTIEAASKNELLNTEFLHRMMGYVKCHNISLEKAILKAPITKENFSHILKALQMIEPNFNIICNKPELKNKSALKETCISSSIDELEKFFEVQLNNEILTSVNVDPIRNVTVNPEILKKVILIEDTWRSSLTEAYEKKITLLEYSDRSKLYPYLSVLPTKSYVDAMIQVVFQLSTDSELQFNRIQHVHLHIGNIISNMFRHRCLKKQKYFAQLKSICLSYYGKLSDNPQNIEMDKLWESSIMENIDSGSIVHDTIDWSKKIKASIGKILYEIMFENMNFTFYNGITAVTTPVIYEKLEEDAGKVFITLKIQSKLRTIFKAINKALPGFSQLDMPMTHPPVPFHKNHESGFFYLTNYATRIIRKSTIFDIFDDDLEQPDDISKVYNALNVLSLCSWKIENANLDLVMQVFNDSGNMKLKIPTSANKFNGPEKPAVDLPIKDKLNSWKSYFQFVKEQKEMYSLWSTLSYKIMIAQKLKNKPFWYPYSMDFRGRVYPCSPHLSHIGDDVSRGLLRFSLGKKLGPKGLDWIKIHLINLCGTHKRKSNSDRLKAANDVISEVIDSAVNPFDGRCWWQLSEKPWQTLATCREIHKILKFVKSNGKPDDFHSTLPIHQDGSCNGLQHYAALGKDAKGAAAVNLIQSETPQDVYSDVLEHLEVKRKEDAKNGVEEAILLEGLLSRKVIKQTVMTTVYGVTQYGGTLQILRELKNLEKLPPQNIKSCAAYLSKLTLGSIGEIFASATNIQNWFIELSSNISRLRKANISWKTPMNFIVKQPYFADDSDILKSDCLKNKNAFPPNFIHSLDSTHMMLTALSCWEKNITFVSVHDCFWTHACDVDEMNKICREEFVKLHSQPILENLSDYLLEKYQFQINPNENDNGRKIAFNKLLKNIPKKG 1041          
The following BLAST results are available for this feature:
BLAST of DNA-directed RNA polymerase vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 2
Match NameE-valueIdentityDescription
POLRMT4.715e-15933.14RNA polymerase mitochondrial [Source:HGNC Symbol;A... [more]
POLRMT1.755e-3467.95RNA polymerase mitochondrial [Source:HGNC Symbol;A... [more]
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BLAST of DNA-directed RNA polymerase vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 3
Match NameE-valueIdentityDescription
rpom-14.640e-12736.36DNA-directed RNA polymerase [Source:UniProtKB/TrE... [more]
rpom-14.640e-12736.36DNA-directed RNA polymerase [Source:UniProtKB/TrE... [more]
rpom-14.944e-12335.67DNA-directed RNA polymerase [Source:UniProtKB/TrE... [more]
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BLAST of DNA-directed RNA polymerase vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2
Match NameE-valueIdentityDescription
mtRNApol1.323e-15632.52gene:FBgn0261938 transcript:FBtr0332273[more]
mtRNApol1.323e-15632.52gene:FBgn0261938 transcript:FBtr0077989[more]
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BLAST of DNA-directed RNA polymerase vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
polrmt3.203e-16534.39polymerase (RNA) mitochondrial (DNA directed) [Sou... [more]
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BLAST of DNA-directed RNA polymerase vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
pacrg3.125e-16934.04parkin co-regulated [Source:Xenbase;Acc:XB-GENE-62... [more]
pacrg6.033e-16934.04parkin co-regulated [Source:Xenbase;Acc:XB-GENE-62... [more]
pacrg3.987e-16233.79parkin co-regulated [Source:Xenbase;Acc:XB-GENE-62... [more]
pacrg6.429e-16133.94parkin co-regulated [Source:Xenbase;Acc:XB-GENE-62... [more]
pacrg1.023e-16034.07parkin co-regulated [Source:Xenbase;Acc:XB-GENE-62... [more]
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BLAST of DNA-directed RNA polymerase vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 4
Match NameE-valueIdentityDescription
Polrmt3.584e-15633.56polymerase (RNA) mitochondrial (DNA directed) [Sou... [more]
Polrmt1.491e-14544.06polymerase (RNA) mitochondrial (DNA directed) [Sou... [more]
Polrmt3.023e-9942.01polymerase (RNA) mitochondrial (DNA directed) [Sou... [more]
Polrmt2.214e-6927.12polymerase (RNA) mitochondrial (DNA directed) [Sou... [more]
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BLAST of DNA-directed RNA polymerase vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|O00411|RPOM_HUMAN2.264e-15833.14DNA-directed RNA polymerase, mitochondrial OS=Homo... [more]
sp|Q8BKF1|RPOM_MOUSE2.507e-15533.56DNA-directed RNA polymerase, mitochondrial OS=Mus ... [more]
sp|O24600|RPOT3_ARATH4.698e-10338.06DNA-directed RNA polymerase 3, chloroplastic OS=Ar... [more]
sp|Q8L6J3|RPO2B_TOBAC2.310e-9832.88DNA-directed RNA polymerase 2B, chloroplastic/mito... [more]
sp|Q8VWF8|RPOT2_NICSY1.355e-9734.20DNA-directed RNA polymerase 2, chloroplastic/mitoc... [more]
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BLAST of DNA-directed RNA polymerase vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A087VZB80.000e+033.58DNA-directed RNA polymerase OS=Echinococcus multil... [more]
A0A3Q0KPI70.000e+034.60DNA-directed RNA polymerase OS=Schistosoma mansoni... [more]
A0A0X3P3U90.000e+032.62DNA-directed RNA polymerase (Fragment) OS=Schistoc... [more]
A0A068WX420.000e+032.79DNA-directed RNA polymerase OS=Echinococcus granul... [more]
A0A4Z2DVV80.000e+039.25DNA-directed RNA polymerase OS=Schistosoma japonic... [more]
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BLAST of DNA-directed RNA polymerase vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
polrmt1.758e-16334.67RNA polymerase mitochondrial [Source:NCBI gene;Acc... [more]
polrmt6.437e-14945.16RNA polymerase mitochondrial [Source:NCBI gene;Acc... [more]
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BLAST of DNA-directed RNA polymerase vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ENSPMAT00000005603.11.295e-10930.36pep scaffold:Pmarinus_7.0:GL476532:352361:404828:1... [more]
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BLAST of DNA-directed RNA polymerase vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1
Match NameE-valueIdentityDescription
RPO417.396e-9832.19Mitochondrial RNA polymerase; single subunit enzym... [more]
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BLAST of DNA-directed RNA polymerase vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 3
Match NameE-valueIdentityDescription
EDO286651.591e-11247.13DNA-directed RNA polymerase [Source:UniProtKB/TrE... [more]
EDO417021.634e-10541.50DNA-directed RNA polymerase [Source:UniProtKB/TrE... [more]
EDO477524.194e-4742.97Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of DNA-directed RNA polymerase vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
polrmt5.337e-17234.68RNA polymerase mitochondrial [Source:NCBI gene;Acc... [more]
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BLAST of DNA-directed RNA polymerase vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000026344.10.000e+099.53SMESG000026344.1[more]
SMESG000026344.10.000e+099.52SMESG000026344.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30028902 ID=SMED30028902|Name=DNA-directed RNA polymerase|organism=Schmidtea mediterranea sexual|type=transcript|length=3505bp
GTCGGTTTTTGAATATTCATGCTTCATTTTGTTAGAGCCATTAAATTAAT
TCCAAAAATCAATATTAAATTCTATTCTAACTTTAATCAAGATTTTAAGC
CTCATTTTCATTCAAACAATAATTTGCATGGATGTATTAATCATAACATA
AAATTATGTGATTTAAAAATCGAGGAGATAAATCAAAAAATTGAATTGAT
TGAATTTCAACCATATGAAAAGTTTTTCTTTAAAGACAAAGTTGTACCAA
ATTTATTTCAAAAACCGACTGAAGAAATTTCAAAACCTATTGATATGAAA
TATGTGAATATCATAGAGGAGATCAATCAATTAAAAACTGAAGAATTTTT
GCCTAAGAAACTCGAAGAAGCGATTAATATTACTAGCCTTAAGTTGTTAA
AATTTTTCCCACATTATAAACATCAAAAAGTTAAAGAATTAAACAATGTT
TCTGCTCTACGGTTCATTAAAAGTCTCGTTTATAACACTAATAACCATGC
GACATATATGGATGTGTGTATACAAAAAGGAGACTATGAAAAGGCACTTA
GTTTATTCAATTCTGTTAGAAATGAGAATGAAACCTATCATTCAGTTGAT
AAAAAGTCAATTATTGTCGATTCGGTTTTCTACGAACCAATTATGAAATT
GCTCGCGAAAAACGGGAAAATAGATGCTATTATCACACTTTTAAATGAAA
TGGATGAATTTCATATTTTGCCAAATATATCGATAATTTTCCGAACAATC
GAAGCTGCTAGTAAAAATGAATTATTGAATACAGAGTTTCTCCACCGAAT
GATGGGGTATGTTAAATGCCATAATATATCATTGGAGAAAGCCATTTTAA
AAGCTCCAATAACAAAAGAAAATTTTAGTCACATATTGAAAGCTCTTCAA
ATGATAGAACCGAATTTCAATATCATTTGTAACAAACCAGAATTGAAAAA
TAAATCTGCATTGAAAGAAACTTGCATATTTTCATCGATCGATGAATTGG
AAAAATTTTTCGAAGTTCAACTAAACAATGAAATATTAACCAGCGTAAAT
GTCGATCCGATACACAATGTAACAGTTAATCCAGAAATTCTGAAAAAAGT
CATATTAATCGAAGACACCTGGCGATCGAGTCTTACAGAAGCGTATGAGA
AAAAAATCACTCTTCTAGAGTATTCTGATCGCTCAAAATTGTATCCGTAT
TTATCGGTGTTACCAACAAAATCCTATGTCGATGCAATGATACAAGTCGT
CTTTCAGCTGTCAACCGATTCTGAATTACAATTCAACAGAATTCAACACG
TTCACTTGCATATTGGAAATATTATCAGTAACATGTTTCGTCATCGCTGT
CTGAAAAAACAGAAATATTTCGCCCAATTAAAATCGATCTGCTTGTCGTA
TTACGGAAAATTATCGGAAAACCCTCAAAACATAGAAATGGACAAATTAT
GGGAATCATCGATTATGGAAAATATCGATTCGGGGAGTATTGTCCACGAT
ACAATCGATTGGAGTAAAAAAATCAAAGCTTCAATTGGAAAAATTCTCTA
TGAAATCATGTTTGAAAATATGAATTTCACATTCTATAACGGAATAACAG
CGGTAACGACTCCGGTTATCTATGAAAAACTCGAAGAGGATGCTGGAAAA
GTGTTTATCACTTTAAAAATCCAATCGAAACTAAGAACGATATTTAAAGC
GATCAATAAAGCGTTACCGGGTTTCTCTCAGTTAGACATGCCGATGACCC
ACCCGCCAGTTCCATTTCACAAAAATCACGAATCGGGATTTTTCTACTTG
ACAAATTACGCAACGAGAATCATAAGAAAATCGACAATTTTCGATATTTT
CGATGACGACCTCGAGCAACCGGATGATATCAGCAAAGTCTATAATGCAC
TCAATGTACTGAGTTTATGCTCATGGAAAATCGAAAACGCGAATTTGGAT
TTGGTTATGCAGGTTTTCAATGACAGTGGAAACATGAAATTGAAAATTCC
CACCTCAGCAAATAAATTTAATGGGCCCGAAAAACCAGCGGTTGATTTGC
CGATCAAGGACAAATTGAATAGTTGGAAGTCGTATTTTCAGTTTGTTAAA
GAACAGAAAGAAATGTATTCACTCTGGTCGACATTGTCGTATAAAATCAT
GATTGCTCAGAAATTAAAAAACAAACCGTTCTGGTATCCGTATTCGATGG
ATTTCCGGGGTAGGGTTTATCCGTGCTCGCCACATTTAAGTCATATCGGT
GATGACGTCAGTAGAGGATTACTTAGATTTAGTCTCGGTAAAAAATTAGG
TCCAAAGGGATTGGATTGGATAAAAATTCATTTGATTAATCTTTGTGGAA
CTCACAAAAGAAAATCTAATAGCGACAGATTAAAAGCAGCCAATGAGGTG
ATTTCAGAAGTTATTGATTCAGCAGCTAATCCTTTTGATGGCCGCTGCTG
GTGGCAATTATCTGAAAAACCCTGGCAAACCTTAGCAACGTGCCGGGAAA
TTCATAAGATTTTAAAGTTTGTTAAAAGCAATGGCAAACCTGATGACTTT
CACTCTACTTTACCCATTCATCAAGATGGATCATGTAATGGCCTACAGCA
TTATGCTGCTTTAGGGAAGGATGCTAAAGGCGCAGCTGCTGTAAACTTGA
TCCAGTCAGAAACACCACAAGATGTTTATTCAGATGTTTTAGAACATCTA
GAAGTGAAACGAAAAGAAGATGCTAAAAATGGTGTTGAAGAAGCAATTTT
ATTGGAAGGATTATTATCTCGAAAAGTTATCAAACAAACGGTTATGACAA
CTGTATACGGTGTAACTCAATATGGAGGAACTTTACAGATTTTACGCGAA
TTGAAAAATCTTGAAAAACTGCCACCGCAAAATATCAAATCGTGTGCTGC
TTATTTATCCAAATTAACATTGGGTTCAATCGGAGAAATTTTTGCTTCAG
CAACTAATATTCAAAATTGGTTTATTGAGTTGTCTTCTAACATCAGTAGA
TTACGCAAAGCCAATATTTCCTGGAAAACACCAATGAATTTTATAGTGAA
ACAACCATATTTTGCTGATGATAGTGACATTTTGAAATCAGATTGTCTCA
AAAATAAAAATGCATTTCCTCCTAACTTTATTCATTCCCTTGATTCGACT
CACATGATGTTGACGGCTCTGAGTTGCTGGGAAAAAAACATAACATTCGT
ATCAGTACACGATTGTTTTTGGACACATGCTTGCGATGTTGATGAAATGA
ATAAGATTTGCAGAGAAGAATTTGTAAAATTACATTCGCAACCAATTCTT
GAAAATTTATCAGATTATCTTTTAGAAAAGTATCAATTCCAGATTAATCC
AAATGAAAATGACAATGGCAGAAAAATAGCATTTAATAAATTATTAAAAA
ATATACCGAAAAAGGGAACTTTCGATATCAGAGAAGTTTTGGACTCGACT
TATTTTTTTAGTTGAAATGTTTTTAGAAAAAATAAAAATTCAGCATTTAA
AAAAA
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protein sequence of SMED30028902-orf-1

>SMED30028902-orf-1 ID=SMED30028902-orf-1|Name=SMED30028902-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=1149bp
MLHFVRAIKLIPKINIKFYSNFNQDFKPHFHSNNNLHGCINHNIKLCDLK
IEEINQKIELIEFQPYEKFFFKDKVVPNLFQKPTEEISKPIDMKYVNIIE
EINQLKTEEFLPKKLEEAINITSLKLLKFFPHYKHQKVKELNNVSALRFI
KSLVYNTNNHATYMDVCIQKGDYEKALSLFNSVRNENETYHSVDKKSIIV
DSVFYEPIMKLLAKNGKIDAIITLLNEMDEFHILPNISIIFRTIEAASKN
ELLNTEFLHRMMGYVKCHNISLEKAILKAPITKENFSHILKALQMIEPNF
NIICNKPELKNKSALKETCIFSSIDELEKFFEVQLNNEILTSVNVDPIHN
VTVNPEILKKVILIEDTWRSSLTEAYEKKITLLEYSDRSKLYPYLSVLPT
KSYVDAMIQVVFQLSTDSELQFNRIQHVHLHIGNIISNMFRHRCLKKQKY
FAQLKSICLSYYGKLSENPQNIEMDKLWESSIMENIDSGSIVHDTIDWSK
KIKASIGKILYEIMFENMNFTFYNGITAVTTPVIYEKLEEDAGKVFITLK
IQSKLRTIFKAINKALPGFSQLDMPMTHPPVPFHKNHESGFFYLTNYATR
IIRKSTIFDIFDDDLEQPDDISKVYNALNVLSLCSWKIENANLDLVMQVF
NDSGNMKLKIPTSANKFNGPEKPAVDLPIKDKLNSWKSYFQFVKEQKEMY
SLWSTLSYKIMIAQKLKNKPFWYPYSMDFRGRVYPCSPHLSHIGDDVSRG
LLRFSLGKKLGPKGLDWIKIHLINLCGTHKRKSNSDRLKAANEVISEVID
SAANPFDGRCWWQLSEKPWQTLATCREIHKILKFVKSNGKPDDFHSTLPI
HQDGSCNGLQHYAALGKDAKGAAAVNLIQSETPQDVYSDVLEHLEVKRKE
DAKNGVEEAILLEGLLSRKVIKQTVMTTVYGVTQYGGTLQILRELKNLEK
LPPQNIKSCAAYLSKLTLGSIGEIFASATNIQNWFIELSSNISRLRKANI
SWKTPMNFIVKQPYFADDSDILKSDCLKNKNAFPPNFIHSLDSTHMMLTA
LSCWEKNITFVSVHDCFWTHACDVDEMNKICREEFVKLHSQPILENLSDY
LLEKYQFQINPNENDNGRKIAFNKLLKNIPKKGTFDIREVLDSTYFFS*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000429neoblast
PLANA:0002109X1 cell
PLANA:0002111X2 cell
Vocabulary: INTERPRO
TermDefinition
IPR011990TPR-like_helical_dom_sf
IPR029262RPOL_N
IPR002092DNA-dir_Rpol_phage-type
IPR024075DNA-dir_RNA_pol_helix_hairpin
IPR002885Pentatricopeptide_repeat
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
GO:0003899DNA-directed 5'-3' RNA polymerase activity
GO:0003677DNA binding
GO:0016740transferase activity
GO:0016779nucleotidyltransferase activity
Vocabulary: biological process
TermDefinition
GO:0006351transcription, DNA-templated
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029262DNA-directed RNA polymerase, N-terminalSMARTSM01311RPOL_N_2coord: 332..633
e-value: 1.6E-7
score: 6.6
IPR029262DNA-directed RNA polymerase, N-terminalPFAMPF14700RPOL_Ncoord: 367..632
e-value: 3.4E-18
score: 66.1
NoneNo IPR availableGENE3DG3DSA:1.10.150.20coord: 870..1048
e-value: 2.8E-127
score: 427.1
NoneNo IPR availableGENE3DG3DSA:1.10.287.280coord: 758..843
e-value: 2.8E-127
score: 427.1
NoneNo IPR availableGENE3DG3DSA:3.30.70.370coord: 728..1108
e-value: 2.8E-127
score: 427.1
NoneNo IPR availablePANTHERPTHR10102:SF0DNA-DIRECTED RNA POLYMERASE, MITOCHONDRIALcoord: 283..1148
NoneNo IPR availableSUPERFAMILYSSF56672DNA/RNA polymerasescoord: 367..1148
IPR002092DNA-directed RNA polymerase, phage-typePFAMPF00940RNA_polcoord: 758..1148
e-value: 1.0E-140
score: 469.3
IPR002092DNA-directed RNA polymerase, phage-typePANTHERPTHR10102DNA-DIRECTED RNA POLYMERASE, MITOCHONDRIALcoord: 283..1148
IPR002092DNA-directed RNA polymerase, phage-typePROSITEPS00900RNA_POL_PHAGE_1coord: 849..860
IPR002092DNA-directed RNA polymerase, phage-typePROSITEPS00489RNA_POL_PHAGE_2coord: 916..930
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3DG3DSA:1.25.40.10coord: 128..300
e-value: 1.6E-8
score: 36.5
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 201..235
score: 8.977
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 156..190
score: 6.61