Pyrazinamidase/nicotinamidase, putative

Overview
NamePyrazinamidase/nicotinamidase, putative
Smed IDSMED30028895
Length (bp)3038
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Pyrazinamidase/nicotinamidase, putative (SMED30028895) t-SNE clustered cells

Violin plots show distribution of expression levels for Pyrazinamidase/nicotinamidase, putative (SMED30028895) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Pyrazinamidase/nicotinamidase, putative (SMED30028895) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Pyrazinamidase/nicotinamidase, putative (SMED30028895) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30028895

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30028895SMESG000005583.1 dd_Smed_v4_11420_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30028895SMESG000005583.1 dd_Smed_v4_11420_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30028895SMESG000005583.1 dd_Smed_v4_11420_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Pyrazinamidase/nicotinamidase, putative vs. Ensembl Celegans
Match: pnc-2 (Pyrazinamidase and NiCotinamidase [Source:UniProtKB/TrEMBL;Acc:Q7YTI0])

HSP 1 Score: 135.961 bits (341), Expect = 8.130e-35
Identity = 81/230 (35.22%), Postives = 126/230 (54.78%), Query Frame = 1 
Query: 2026 EEFQEL-CLGLFEPFLSPKSAFIIVDVQNDFINGSLALKNCPANEDGAEVIPMINSILTRFIEGDYKFKSVVYTYDWHPKDHISFFDNIHCRRISQHSPISLIDVKPLDKVIFETDSGEETDQILWPKHCVENSDGAALHKDLMVIDGAYHLKKGIFSNVDSYSAFGLNHPKAVTDLNNHLKSHDINFLFVCGLATDVCVAQTALDGLSLGYRVALVKDACRGVNQENID 2712
            E+ QEL    + +PF + +++ ++VD QNDF+ GSL++K   A +D  E +P +N++L         +  +VYT DWHP +HISFF++                ++P D V F       T Q+L+P HC++   G+ LH  L  IDGA+++KKG    VD+YSAF  N     ++L   L+ +DIN +  CGLA D+CV  T  D    G+   +VK   +G++   +D
Sbjct:   46 EDTQELFTRVVLKPFTTLRTSLLVVDFQNDFVTGSLSIKEGDAEQDPLEALPHVNNLLEN-----LNWNMIVYTQDWHPSNHISFFEHARNPDRELAPEDKSRKLRPFDIVRFVKPVS--TIQVLYPSHCIQGGWGSQLHLGLQRIDGAHYIKKGADVYVDAYSAFSDNCGIKQSELEMLLRKNDINAVIGCGLAYDICVMHTLKDASKHGFLTCIVKSGSKGLSSLKMD 268
[Close]
BLAST of Pyrazinamidase/nicotinamidase, putative vs. Ensembl Celegans
Match: pnc-1 (Pyrazinamidase and NiCotinamidase [Source:UniProtKB/TrEMBL;Acc:A7WK39])

HSP 1 Score: 124.02 bits (310), Expect = 1.145e-30
Identity = 69/219 (31.51%), Postives = 124/219 (56.62%), Query Frame = 1 
Query: 2071 SPKSAFIIVDVQNDFINGSLALKNCPANEDGAEVIPMINSILTRFIEGDYKFKSVVYTYDWHPKDHISFFDNIHCRRISQHSPISLIDVKPLDKVIFETDSGE-----ETDQILWPKHCVENSDGAALHKDLMVIDGAYHLKKGIFSNVDSYSAFGLNHPKAVTDLNNHLKSHDINFLFVCGLATDVCVAQTALDGLSLGYRVALVKDACRGVNQENID 2712
            S + A ++VD QNDF++GSL + +  A ++ +  I  +N +L         +  VVYT DWHP +HISF           H+   ++D K  +K +   DS +     +T+Q+L+P HC++ S G+ +H ++ + D A ++ KG+   +DSYSAF  N+ ++ T+L + L+  +I+ + + GLA D+CV  T LD +   +  A++ +   G+ ++ I+
Sbjct:   70 SLRVALLVVDFQNDFVDGSLKIGDGDAGQEPSSAITPLNELLQL-----SSWDLVVYTKDWHPHNHISFLSQ-------AHNSDRVMDEKDENKTLGFFDSVQFLKPIKTEQVLYPDHCIQKSWGSDIHPEIYIADNAEYIMKGVDPYLDSYSAFNDNNGRSKTELEDLLRRENIDAVVIAGLAYDICVRFTCLDAVKQNFLAAVIPECSAGLTKKGIE 276
[Close]
BLAST of Pyrazinamidase/nicotinamidase, putative vs. Ensembl Celegans
Match: pnc-1 (Pyrazinamidase and NiCotinamidase [Source:UniProtKB/TrEMBL;Acc:A7WK39])

HSP 1 Score: 124.02 bits (310), Expect = 1.145e-30
Identity = 69/219 (31.51%), Postives = 124/219 (56.62%), Query Frame = 1 
Query: 2071 SPKSAFIIVDVQNDFINGSLALKNCPANEDGAEVIPMINSILTRFIEGDYKFKSVVYTYDWHPKDHISFFDNIHCRRISQHSPISLIDVKPLDKVIFETDSGE-----ETDQILWPKHCVENSDGAALHKDLMVIDGAYHLKKGIFSNVDSYSAFGLNHPKAVTDLNNHLKSHDINFLFVCGLATDVCVAQTALDGLSLGYRVALVKDACRGVNQENID 2712
            S + A ++VD QNDF++GSL + +  A ++ +  I  +N +L         +  VVYT DWHP +HISF           H+   ++D K  +K +   DS +     +T+Q+L+P HC++ S G+ +H ++ + D A ++ KG+   +DSYSAF  N+ ++ T+L + L+  +I+ + + GLA D+CV  T LD +   +  A++ +   G+ ++ I+
Sbjct:   70 SLRVALLVVDFQNDFVDGSLKIGDGDAGQEPSSAITPLNELLQL-----SSWDLVVYTKDWHPHNHISFLSQ-------AHNSDRVMDEKDENKTLGFFDSVQFLKPIKTEQVLYPDHCIQKSWGSDIHPEIYIADNAEYIMKGVDPYLDSYSAFNDNNGRSKTELEDLLRRENIDAVVIAGLAYDICVRFTCLDAVKQNFLAAVIPECSAGLTKKGIE 276
[Close]
BLAST of Pyrazinamidase/nicotinamidase, putative vs. Ensembl Celegans
Match: pnc-1 (Pyrazinamidase and NiCotinamidase [Source:UniProtKB/TrEMBL;Acc:A7WK39])

HSP 1 Score: 123.635 bits (309), Expect = 1.897e-30
Identity = 69/219 (31.51%), Postives = 124/219 (56.62%), Query Frame = 1 
Query: 2071 SPKSAFIIVDVQNDFINGSLALKNCPANEDGAEVIPMINSILTRFIEGDYKFKSVVYTYDWHPKDHISFFDNIHCRRISQHSPISLIDVKPLDKVIFETDSGE-----ETDQILWPKHCVENSDGAALHKDLMVIDGAYHLKKGIFSNVDSYSAFGLNHPKAVTDLNNHLKSHDINFLFVCGLATDVCVAQTALDGLSLGYRVALVKDACRGVNQENID 2712
            S + A ++VD QNDF++GSL + +  A ++ +  I  +N +L         +  VVYT DWHP +HISF           H+   ++D K  +K +   DS +     +T+Q+L+P HC++ S G+ +H ++ + D A ++ KG+   +DSYSAF  N+ ++ T+L + L+  +I+ + + GLA D+CV  T LD +   +  A++ +   G+ ++ I+
Sbjct:   90 SLRVALLVVDFQNDFVDGSLKIGDGDAGQEPSSAITPLNELLQL-----SSWDLVVYTKDWHPHNHISFLSQ-------AHNSDRVMDEKDENKTLGFFDSVQFLKPIKTEQVLYPDHCIQKSWGSDIHPEIYIADNAEYIMKGVDPYLDSYSAFNDNNGRSKTELEDLLRRENIDAVVIAGLAYDICVRFTCLDAVKQNFLAAVIPECSAGLTKKGIE 296
[Close]
BLAST of Pyrazinamidase/nicotinamidase, putative vs. Ensembl Fly
Match: Naam (gene:FBgn0051216 transcript:FBtr0083786)

HSP 1 Score: 198.364 bits (503), Expect = 7.018e-56
Identity = 118/313 (37.70%), Postives = 170/313 (54.31%), Query Frame = 1 
Query: 1900 LNLEDFSELFNIFFNGCNDK------YTQNIKECFEYFDKDKDQLLNFEEFQELCLGLFEPFLSPKSAFIIVDVQNDFINGSLALKNCPANEDGAEVIPMINSILTRFIEGDYKFKSVVYTYDWHPKDHISFFDNIHCRRISQHSPISLIDVKPLDKVIFETDSGEETDQILWPKHCVENSDGAALHKDLMVIDGAYHLKKGIFSNVDSYSAFGLNHPKAVTDLNNHLKSHDINFLFVCGLATDVCVAQTALDGLSLGYRVALVKDACRGVNQENIDNVQCKLIENXXXXXXXXXXXXXLNGRVKPVQLAVSL 2820
            L+L +FS +    F   NDK        + +++ F  FD + D  ++ EEF+       +  + P +AF+IVDVQNDFI+GSL + NC A + G E++  IN +L         F +V Y+ DWHP DH+SF DN+  R + + S +     K  D VIF         Q LWP+HCV++S GA LHKDL V+D    + KG    VDSYS F  N   + T LN  LK      ++VCGLA DVCV  TA+D LS GYR  L+ D CRG +  +I++ + K+  +  +I+ +  +  +  GR +  +L   L
Sbjct:   33 LSLAEFSIICRALFR--NDKGHIYDVPPERLEQIFAVFDTNGDGFIDREEFKFCWNQWIKTIVRPVNAFLIVDVQNDFISGSLDISNCSAQQQGHEILEPINKLLDTV-----DFDAVFYSLDWHPSDHVSFIDNVKMRPMDESSALDSDSAKVFDTVIFA--GPPPMKQRLWPRHCVQDSWGAELHKDLKVVDHGIKVYKGTNPEVDSYSVFWDNKKLSDTTLNAQLKMKGATDIYVCGLAYDVCVGATAVDALSAGYRTILIDDCCRGTDVHDIEHTKEKVNTSDGVIVHTNQVKAMAEGRDRRPELGYKL 336
[Close]
BLAST of Pyrazinamidase/nicotinamidase, putative vs. Ensembl Fly
Match: Naam (gene:FBgn0051216 transcript:FBtr0335415)

HSP 1 Score: 198.364 bits (503), Expect = 7.018e-56
Identity = 118/313 (37.70%), Postives = 170/313 (54.31%), Query Frame = 1 
Query: 1900 LNLEDFSELFNIFFNGCNDK------YTQNIKECFEYFDKDKDQLLNFEEFQELCLGLFEPFLSPKSAFIIVDVQNDFINGSLALKNCPANEDGAEVIPMINSILTRFIEGDYKFKSVVYTYDWHPKDHISFFDNIHCRRISQHSPISLIDVKPLDKVIFETDSGEETDQILWPKHCVENSDGAALHKDLMVIDGAYHLKKGIFSNVDSYSAFGLNHPKAVTDLNNHLKSHDINFLFVCGLATDVCVAQTALDGLSLGYRVALVKDACRGVNQENIDNVQCKLIENXXXXXXXXXXXXXLNGRVKPVQLAVSL 2820
            L+L +FS +    F   NDK        + +++ F  FD + D  ++ EEF+       +  + P +AF+IVDVQNDFI+GSL + NC A + G E++  IN +L         F +V Y+ DWHP DH+SF DN+  R + + S +     K  D VIF         Q LWP+HCV++S GA LHKDL V+D    + KG    VDSYS F  N   + T LN  LK      ++VCGLA DVCV  TA+D LS GYR  L+ D CRG +  +I++ + K+  +  +I+ +  +  +  GR +  +L   L
Sbjct:   33 LSLAEFSIICRALFR--NDKGHIYDVPPERLEQIFAVFDTNGDGFIDREEFKFCWNQWIKTIVRPVNAFLIVDVQNDFISGSLDISNCSAQQQGHEILEPINKLLDTV-----DFDAVFYSLDWHPSDHVSFIDNVKMRPMDESSALDSDSAKVFDTVIFA--GPPPMKQRLWPRHCVQDSWGAELHKDLKVVDHGIKVYKGTNPEVDSYSVFWDNKKLSDTTLNAQLKMKGATDIYVCGLAYDVCVGATAVDALSAGYRTILIDDCCRGTDVHDIEHTKEKVNTSDGVIVHTNQVKAMAEGRDRRPELGYKL 336
[Close]
BLAST of Pyrazinamidase/nicotinamidase, putative vs. UniProt/SwissProt
Match: sp|P21369|PNCA_ECOLI (Nicotinamidase OS=Escherichia coli (strain K12) OX=83333 GN=pncA PE=1 SV=1)

HSP 1 Score: 118.627 bits (296), Expect = 8.432e-29
Identity = 81/221 (36.65%), Postives = 110/221 (49.77%), Query Frame = 1 
Query: 2074 PKSAFIIVDVQNDFINGSLALKNCPANEDGAEVIPMINS---ILTRFIEG-DYKFKSVVYTYDWHPKDHISFFDNIHCRRISQHSPISLIDVKPLDKVIFETDSGEETDQILWPKHCVENSDGAALHKDLMVIDGAYHLKKGIFSNVDSYSAFGLNHPKAVTDLNNHLKSHDINFLFVCGLATDVCVAQTALDGLSLGYRVALVKDACRGVNQENIDNVQC 2724
            P  A ++VD+QNDF  G            GA  +P  +S   +  R I+    + ++V+ + DWHP +H SF         SQH       V+P     +     +   Q  WP HCV+NS+GA LH  L     A    KG    VDSYSAF  N  +  T L++ L+ H+I+ L V GLATD CV  T LD L LGY+V ++ D CRGVN +  D+   
Sbjct:    2 PPRALLLVDLQNDFCAG------------GALAVPEGDSTVDVANRLIDWCQSRGEAVIASQDWHPANHGSF--------ASQHG------VEP-----YTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLHQKAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFTVLDALQLGYKVNVITDGCRGVNIQPQDSAHA 191
[Close]
BLAST of Pyrazinamidase/nicotinamidase, putative vs. UniProt/SwissProt
Match: sp|P53184|PNC1_YEAST (Nicotinamidase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=PNC1 PE=1 SV=1)

HSP 1 Score: 98.5969 bits (244), Expect = 7.704e-22
Identity = 75/227 (33.04%), Postives = 114/227 (50.22%), Query Frame = 1 
Query: 2083 AFIIVDVQNDFIN--GSLALKNCPANEDGAEVIPMINSILTRFIEGDYKFKSVVYTYDWHPKDHISFFDNIHCRR----ISQHSPISLIDVKPLDKVIFETDSGEETDQILWPKHCVENSDGAALHKDLMVIDGAYHLK---KGIFSNVDSYSAFGLNHPKAVTDLNNHLKSHDINFLFVCGLATDVCVAQTALDGLSLGYRVALVKDACRGV--NQENIDNVQCKL 2730
              I+VD+QNDFI+  GSL +   P  E+      +IN I     + D  +  +V T DWHP  HISF  N   +      + HSP      +P D      DS +E   ILWP HCV+N+ G+ L   +M      H+K   KG  ++ + YSAF        TD+N +L+ H  + +++ G+A + CV  TA+    LGY+  ++ D  R +  + E I+ V+ +L
Sbjct:    3 TLIVVDMQNDFISPLGSLTV---PKGEE------LINPISDLMQDADRDWHRIVVTRDWHPSRHISFAKNHKDKEPYSTYTYHSP------RPGD------DSTQEG--ILWPVHCVKNTWGSQLVDQIMDQVVTKHIKIVDKGFLTDREYYSAFHDIWNFHKTDMNKYLEKHHTDEVYIVGVALEYCVKATAISAAELGYKTTVLLDYTRPISDDPEVINKVKEEL 206
[Close]
BLAST of Pyrazinamidase/nicotinamidase, putative vs. UniProt/SwissProt
Match: sp|Q9USS0|PNC1_SCHPO (Nicotinamidase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=pnc1 PE=3 SV=2)

HSP 1 Score: 95.1301 bits (235), Expect = 1.261e-20
Identity = 73/213 (34.27%), Postives = 101/213 (47.42%), Query Frame = 1 
Query: 2083 AFIIVDVQNDFINGSLALKNCPANEDGAEVIPMINSILTRFIEGDYKFKSVVYTYDWHPKDHISFFDNIHCRRISQHSPISLIDVKPLDKVIFETDSGEETDQILWPKHCVENSDGAALHKDLMVIDGAYHLKKGIFSNVDSYSAF-----GLNHPKAVTDLNNHLKSHDINFLFVCGLATDVCVAQTALDGLSLGYRVALVKDACRGVNQEN 2706
            A IIVDVQNDF++         + E   EV+P+IN    R +E DYK+ +V+ T D HPKDH+SF         + HS       KP   V+     G    Q LW  HCVEN+ G      L      + + KG    V+SYS F       N  KA+ D         I  +F+ G+ATD+CV +TAL      Y   ++ +A +G + E+
Sbjct:    6 ALIIVDVQNDFVHPVY----ISSGESALEVVPVIN----RLLENDYKWDTVIATKDVHPKDHLSF--------TTSHSSTP----KPSGTVVNIEAYGHVYKQTLWNSHCVENTPGCEFPDSLNGDRIEFVIPKGSDRLVESYSGFYDAIGRDNGLKAILDKKG------ITDVFIAGVATDICVKETALHARHW-YNTYIISEAVKGSSTES 191
[Close]
BLAST of Pyrazinamidase/nicotinamidase, putative vs. UniProt/SwissProt
Match: sp|I6XD65|PNCA_MYCTU (Nicotinamidase/pyrazinamidase OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=pncA PE=1 SV=1)

HSP 1 Score: 59.3066 bits (142), Expect = 1.234e-8
Identity = 63/214 (29.44%), Postives = 88/214 (41.12%), Query Frame = 1 
Query: 2083 AFIIVDVQNDFI-NGSLALKNCPANEDGAEVIPMINSILTRFIEGDYKFKSVVYTYDWH--PKDHISFFDNIHCRRISQHSPISLIDVKPLDKVIFETDSGEETDQILWPKHCVENSDGAALHK--DLMVIDGAYHLKKGIFSNVDSYSAF-GLNHPKAVTDLNNHLKSHDINFLFVCGLATDVCVAQTALDGLSLGYRVALVKDACRGVNQEN 2706
            A IIVDVQNDF   GSLA+        GA +   I+  L    +    +  VV T D+H  P DH S                                 G       WP HCV  + GA  H   D   I+  ++  KG ++   +YS F G++     T L N L+   ++ + V G+ATD CV QTA D +  G    ++ D   GV+ + 
Sbjct:    3 ALIIVDVQNDFCEGGSLAVTG------GAALARAISDYLAEAAD----YHHVVATKDFHIDPGDHFS---------------------------------GTPDYSSSWPPHCVSGTPGADFHPSLDTSAIEAVFY--KGAYTG--AYSGFEGVDENG--TPLLNWLRQRGVDEVDVVGIATDHCVRQTAEDAVRNGLATRVLVDLTAGVSADT 167
[Close]
BLAST of Pyrazinamidase/nicotinamidase, putative vs. UniProt/SwissProt
Match: sp|O32091|PNCA_BACSU (Uncharacterized isochorismatase family protein PncA OS=Bacillus subtilis (strain 168) OX=224308 GN=pncA PE=3 SV=1)

HSP 1 Score: 58.9214 bits (141), Expect = 1.843e-8
Identity = 39/126 (30.95%), Postives = 62/126 (49.21%), Query Frame = 1 
Query: 2347 DKVIFETDSGEETDQI-----LWPKHCVENSDGAALHKDLMVIDGAYHLKKGIF-SNVDSYSAFGLNHPKAVTDLNNHLKSHDINFLFVCGLATDVCVAQTALDGLSLGYRVALVKDACRGVNQEN 2706
            D V+   DS +E DQ      L+P H ++ ++G  L+  L+ +   +  +  ++      YSAF      A TDL   L+   I  L + G+ TD+CV  TA+D  + G+R+ + K A    NQE 
Sbjct:   46 DYVVLAVDSHDEGDQYHPETRLFPPHNIKGTEGKDLYGKLLPLYQKHEHEPNVYYMEKTRYSAF------AGTDLELKLRERQIGELHLAGVCTDICVLHTAVDAYNKGFRIVVHKQAVASFNQEG 165
[Close]
BLAST of Pyrazinamidase/nicotinamidase, putative vs. TrEMBL
Match: A0A2P6KV78 (PncA OS=Nephila clavipes OX=2585209 GN=NCL1_27050 PE=4 SV=1)

HSP 1 Score: 241.506 bits (615), Expect = 5.994e-68
Identity = 129/302 (42.72%), Postives = 181/302 (59.93%), Query Frame = 1 
Query: 1846 EVTVVDILEWFNAIKFSSLNLEDFSELFNIFF---NGCNDKYTQNI-KECFEYFDKDKDQLLNFEEFQELCLGLFEPFLSPKSAFIIVDVQNDFINGSLALKNCPANEDGAEVIPMINSILTRFIEGDYKFKSVVYTYDWHPKDHISFFDNIHCRRISQHSPISLIDVKPLDKVIFETDSGEETDQILWPKHCVENSDGAALHKDLMVIDGAYHLKKGIFSNVDSYSAFGLNHPKAVTDLNNHLKSHDINFLFVCGLATDVCVAQTALDGLSLGYRVALVKDACRGVNQENIDNVQCKLIEN 2739
            E  +VD ++ F+     +LNL +   L +  F   NG   ++   +  E F  FD D+D  +N EEF+EL   +  P + P +A I +DVQNDFI+GSLA++NCPA +DG EV+P+IN +L         FK V YTYDWHP+DH+SF DN+H R   +   +   + K  D VIFE       +Q LWP+HCV+ S G+ LH +L + D A  + KG  S++DSYSAF  N   + T+L   L+S  +  +FV GLAT+ CV  TAL  L  GYR  LV+DACRGV+ ++I+  + KL+E 
Sbjct:   20 EQIIVDCMQHFDRDGDGTLNLRELCSLTDELFLDENGVAYEFPVRMCAEVFYAFDTDRDGKINMEEFKELWFKVIVPVIKPTTALIAIDVQNDFIDGSLAIRNCPAGQDGYEVVPVINDLLETV-----PFKYVFYTYDWHPEDHVSFIDNVHLRNFHESCSVPHDEAKIFDTVIFE--GPPPIEQKLWPRHCVQTSWGSELHSELKIADNATFIYKGTCSDIDSYSAFWDNQKLSQTNLAEELRSRGVTDVFVSGLATEYCVGYTALHALEHGYRTILVEDACRGVDVKDIEETKRKLVER 314
[Close]
BLAST of Pyrazinamidase/nicotinamidase, putative vs. TrEMBL
Match: T1JPC2 (Uncharacterized protein OS=Strigamia maritima OX=126957 PE=4 SV=1)

HSP 1 Score: 238.039 bits (606), Expect = 6.427e-67
Identity = 124/288 (43.06%), Postives = 177/288 (61.46%), Query Frame = 1 
Query: 1894 SSLNLEDFSELFNIFFNGCNDKYT------QNIKECFEYFDKDKDQLLNFEEFQELCLGLFEPFLSPKSAFIIVDVQNDFINGSLALKNCPANEDGAEVIPMINSILTRFIEGDYKFKSVVYTYDWHPKDHISFFDNIHCRRISQHSPISLIDVKPLDKVIFETDSGEETDQILWPKHCVENSDGAALHKDLMVIDGAYHLKKGIFSNVDSYSAFGLNHPKAVTDLNNHLKSHDINFLFVCGLATDVCVAQTALDGLSLGYRVALVKDACRGVNQENIDNVQCKLIEN 2739
            S LN ++F  L    F  CN+         + ++E F  FD +KD+ ++F EF+       +  LSP SA +++DVQNDFI+GSL+LKNC A +DG ++IP++NS+L         F  VVYTYDWHP +H+SFF+NI  R++ + + + + D K LD VIFE     E  QILWP HC++ S GA LH DL V+D A  + KG+  +VDSYSAF  N   + T L   L+  +I  ++VCGLA DVCVA TA   +  G+R  L+ DACRGV++E ++    ++ +N
Sbjct:   11 SFLNFKEFQALTRALF--CNEDGKPYKVDDKRVEEMFAIFDTNKDKKIDFNEFKVCWNEWIKQILSPVSALLVIDVQNDFISGSLSLKNCAAKQDGNDIIPVVNSLL-----DSVPFDLVVYTYDWHPSNHVSFFENIEMRKLDKSNKVKMADAKVLDTVIFEGPPVNE--QILWPAHCIQGSWGAKLHPDLKVVDKAVEIYKGVNPDVDSYSAFWDNRKMSETKLLKELRDRNITDVYVCGLAFDVCVAHTAFHAIEHGFRTTLIDDACRGVSEEGVERTVARVKQN 289
[Close]
BLAST of Pyrazinamidase/nicotinamidase, putative vs. TrEMBL
Match: A0A131Y2P3 (Putative pyrazinamidase/nicotinamidase pnc1 OS=Ixodes ricinus OX=34613 PE=2 SV=1)

HSP 1 Score: 234.572 bits (597), Expect = 1.732e-65
Identity = 126/287 (43.90%), Postives = 180/287 (62.72%), Query Frame = 1 
Query: 1972 IKECFEYFDKDKDQLLNFEEFQELCLGLFEPFLSPKSAFIIVDVQNDFINGSLALKNCPANEDGAEVIPMINSILTRFIEGDYKFKSVVYTYDWHPKDHISFFDNIHCRRISQHSPISLIDVKPLDKVIFETDSGEE--TDQILWPKHCVENSDGAALHKDLMVIDGAYHLKKGIFSNVDSYSAFGLNHPKAVTDLNNHLKSHDINFLFVCGLATDVCVAQTALDGLSLGYRVALVKDACRGVNQENIDNVQCKLIENXXXXXXXXXXXXXLNGRVKPVQLAVSLIK 2826
            +++ F+ FD+++D  L+F EF+ +      P L PK AF+ VDVQNDFI+GSL+LKN PA  D   V+P+IN +          F  VVY+ DWHPKDHISFF+N HCR     S +S  D + LD V+F     E    +Q LWP HCV+ S GA LHKDL + D A  + KG  ++VDSYSAF  N  ++ T L++ L++H I  +FVCGLA DVCVA TAL  L LGY   LV+ A  G ++E ++ ++ +L +   +I+ S+ ++ +++G+ +P +L V L K
Sbjct:   57 VEKLFKIFDRNQDGKLDFSEFEAMWEQWVVPMLRPKCAFVAVDVQNDFISGSLSLKNIPAGRDPERVVPVINDLTEHL-----PFDVVVYSIDWHPKDHISFFENKHCRAFHSSSKVSAQDARVLDTVVFCDPCSESGCVEQTLWPSHCVQGSWGAQLHKDLRLADKALKIYKGDNTDVDSYSAFWDNARRSETSLHSELRNHGITTVFVCGLAYDVCVAFTALHALELGYGTILVESASCGTSEEAVEKMKRRLQDQLCLIVDSSEVADLVSGKKRPWRLGVQLAK 338
[Close]
BLAST of Pyrazinamidase/nicotinamidase, putative vs. TrEMBL
Match: A0A2R5LCU7 (Putative pyrazinamidase/nicotinamidase pnc1 (Fragment) OS=Ornithodoros turicata OX=34597 PE=4 SV=1)

HSP 1 Score: 233.417 bits (594), Expect = 3.201e-65
Identity = 126/315 (40.00%), Postives = 184/315 (58.41%), Query Frame = 1 
Query: 1894 SSLNLEDFSELFNIFFNGCNDKYT----QNIKECFEYFDKDKDQLLNFEEFQELCLGLFEPFLSPKSAFIIVDVQNDFINGSLALKNCPANEDGAEVIPMINSILTRFIEGDYKFKSVVYTYDWHPKDHISFFDNIHCRRISQHSPISLIDVKPLDKVIFETDSGEET--DQILWPKHCVENSDGAALHKDLMVIDGAYHLKKGIFSNVDSYSAFGLNHPKAVTDLNNHLKSHDINFLFVCGLATDVCVAQTALDGLSLGYRVALVKDACRGVNQENIDNVQCKLIENXXXXXXXXXXXXXLNGRVKPVQLAVSL 2820
            S+L  E+FS      F   ++  T          F+ FD D D  +NF EF+ +      P L PKSA ++VDVQNDFI G+LALKNCPA ED  +++P+IN +  +       +  VVYTYDWHP+DH+SF DN   R + Q S +   + K +D + +   + E    +Q+LWP+HCV+ + GA LH DL+V  G++ + KG+ S +DSYSAF  N   A T L+  L++  +  ++VCGLATDVCV  TA   L LGYR  LV+DAC GV  E I + + +L++ HA++ +S  +  ++    +P    + L
Sbjct:   11 SALEEEEFSRFLARLFTDYDETKTIAGVSAKTHLFKQFDLDHDGKINFTEFENMWTKWVAPVLYPKSAILVVDVQNDFILGTLALKNCPAQEDAEKIVPVINDLTEKV-----PWTMVVYTYDWHPEDHVSFHDNRMKRAVHQSSKVRAEEAKVMDVIRYVAPTLESGYYEQVLWPRHCVQGTWGAELHGDLVVKTGSHKIFKGVNSELDSYSAFWDNSKLASTTLDADLRAAGVTDVYVCGLATDVCVGSTAFHALELGYRTVLVEDACCGVAPEGIRDTKSRLLDQHAVVTTSDKVPGMVEATTRPFAAGLKL 320
[Close]
BLAST of Pyrazinamidase/nicotinamidase, putative vs. TrEMBL
Match: V5IIR0 (Putative pyrazinamidase/nicotinamidase pnc1 OS=Ixodes ricinus OX=34613 PE=2 SV=1)

HSP 1 Score: 230.335 bits (586), Expect = 6.187e-64
Identity = 129/316 (40.82%), Postives = 188/316 (59.49%), Query Frame = 1 
Query: 1900 LNLEDFSELFNIFFNGCNDKYTQNIKE-----CFEYFDKDKDQLLNFEEFQELCLGLFEPFLSPKSAFIIVDVQNDFINGSLALKNCPANEDGAEVIPMINSILTRFIEGDYKFKSVVYTYDWHPKDHISFFDNIHCRRISQHSPISLIDVKPLDKVIFETDSGEE--TDQILWPKHCVENSDGAALHKDLMVIDGAYHLKKGIFSNVDSYSAFGLNHPKAVTDLNNHLKSHDINFLFVCGLATDVCVAQTALDGLSLGYRVALVKDACRGVNQENIDNVQCKLIENXXXXXXXXXXXXXLNGRVKPVQLAVSLIK 2826
            L+ E+F+      F    D  +   KE      F+ FD+++D  L+F EF+ +        L PK AF+ VD+QNDFI+GSL+LKN PA  D   V+P+IN +          F  VVY+ DWHPKDHISFF+N HCR     S +S  D + LD V+F     E    +Q LWP HCV+ S GA LH+DL + D A  + KG  ++VDSYSAF  N  ++ T L++ L++H I  +FVCGLA DVCVA TAL  L LGY   LV++A  G ++E ++ ++ +L +   +I+ S+ ++ +++G+ +P +L + L K
Sbjct:   28 LSKEEFAVFLGSLFTDFEDADSAGDKEDVVEKLFKIFDRNQDGKLDFSEFEAMWEQWVVSMLRPKCAFVAVDIQNDFISGSLSLKNIPAGRDPERVVPVINDLTEHL-----PFDVVVYSIDWHPKDHISFFENKHCRTFHSSSKVSAQDARVLDTVVFCDPCSESGCVEQTLWPSHCVQGSWGAQLHEDLRLADKALKIYKGDKTDVDSYSAFWDNARRSETSLHSELRNHGITTVFVCGLAYDVCVAFTALHALELGYGTILVENASCGTSEEAVEKMKRRLQDQLCLIVDSSEVADLVSGKKRPWRLGLQLAK 338
[Close]
BLAST of Pyrazinamidase/nicotinamidase, putative vs. Ensembl Yeast
Match: PNC1 (Nicotinamidase that converts nicotinamide to nicotinic acid; part of the NAD(+) salvage pathway; required for life span extension by calorie restriction; lacks a peroxisomal targeting signal but is imported into peroxisomes via binding to Gpd1p; PNC1 expression responds to all known stimuli that extend replicative life span; protein increases in abundance and relative distribution to cytoplasmic foci decreases upon DNA replication stress [Source:SGD;Acc:S000003005])

HSP 1 Score: 98.5969 bits (244), Expect = 1.110e-23
Identity = 75/227 (33.04%), Postives = 114/227 (50.22%), Query Frame = 1 
Query: 2083 AFIIVDVQNDFIN--GSLALKNCPANEDGAEVIPMINSILTRFIEGDYKFKSVVYTYDWHPKDHISFFDNIHCRR----ISQHSPISLIDVKPLDKVIFETDSGEETDQILWPKHCVENSDGAALHKDLMVIDGAYHLK---KGIFSNVDSYSAFGLNHPKAVTDLNNHLKSHDINFLFVCGLATDVCVAQTALDGLSLGYRVALVKDACRGV--NQENIDNVQCKL 2730
              I+VD+QNDFI+  GSL +   P  E+      +IN I     + D  +  +V T DWHP  HISF  N   +      + HSP      +P D      DS +E   ILWP HCV+N+ G+ L   +M      H+K   KG  ++ + YSAF        TD+N +L+ H  + +++ G+A + CV  TA+    LGY+  ++ D  R +  + E I+ V+ +L
Sbjct:    3 TLIVVDMQNDFISPLGSLTV---PKGEE------LINPISDLMQDADRDWHRIVVTRDWHPSRHISFAKNHKDKEPYSTYTYHSP------RPGD------DSTQEG--ILWPVHCVKNTWGSQLVDQIMDQVVTKHIKIVDKGFLTDREYYSAFHDIWNFHKTDMNKYLEKHHTDEVYIVGVALEYCVKATAISAAELGYKTTVLLDYTRPISDDPEVINKVKEEL 206
[Close]
BLAST of Pyrazinamidase/nicotinamidase, putative vs. Ensembl Nematostella
Match: EDO44402 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RVL0])

HSP 1 Score: 163.31 bits (412), Expect = 1.641e-45
Identity = 93/220 (42.27%), Postives = 130/220 (59.09%), Query Frame = 1 
Query: 2083 AFIIVDVQNDFINGSLALKNCPANEDGAEVIPMINSILTRFIEGDYKFKSVVYTYDWHPKDHISFFDNIHCRRISQHSPISLIDVKPLDKVIFETDSGEETDQILWPKHCVENSDGAALHKDLMVIDGAYHL-KKGIFSNVDSYSAFGLNHPKAVTDLNNHLKSHDINFLFVCGLATDVCVAQTALDGLSLGYRVALVKDACRGVNQENIDNVQCKLIEN 2739
            A I+VDVQNDFI+GSLALKNCPA EDG +VI  IN +L + +     F  V YT DWHP DH SF DN+    +   SP      K  D +I+     +   Q+LWP HCV +S GA LH+DL   +    + +KG  S++DSYSAF  N   + T+L  +L +H+I  ++VCGLA D CV  TALD  + G++  +++DA   ++   +   + K  E+
Sbjct:    1 ALIVVDVQNDFISGSLALKNCPAGEDGYQVIAPINGLLKKDL-----FDVVAYTLDWHPADHCSFIDNVGLYPVDPSSPTQANAAKVYDTIIYAKP--KPIKQVLWPSHCVIDSWGAELHQDLKAPEKNNIITRKGFDSDLDSYSAFWNNGRLSQTNLFLNLLAHNITNVYVCGLALDYCVQFTALDAATHGFKTYVIEDATAFISHGGLGTAKEKFRES 213
[Close]
BLAST of Pyrazinamidase/nicotinamidase, putative vs. Planmine SMEST
Match: SMESG000005583.1 (SMESG000005583.1)

HSP 1 Score: 698.353 bits (1801), Expect = 0.000e+0
Identity = 355/356 (99.72%), Postives = 356/356 (100.00%), Query Frame = 1 
Query: 1774 MIDIHENDINEFYDRLSKFIGVPGEVTVVDILEWFNAIKFSSLNLEDFSELFNIFFNGCNDKYTQNIKECFEYFDKDKDQLLNFEEFQELCLGLFEPFLSPKSAFIIVDVQNDFINGSLALKNCPANEDGAEVIPMINSILTRFIEGDYKFKSVVYTYDWHPKDHISFFDNIHCRRISQHSPISLIDVKPLDKVIFETDSGEETDQILWPKHCVENSDGAALHKDLMVIDGAYHLKKGIFSNVDSYSAFGLNHPKAVTDLNNHLKSHDINFLFVCGLATDVCVAQTALDGLSLGYRVALVKDACRGVNQENIDNVQCKLIENXXXXXXXXXXXXXLNGRVKPVQLAVSLIKRITNK 2841
            MIDIHENDINEFYDRLSKFIG+PGEVTVVDILEWFNAIKFSSLNLEDFSELFNIFFNGCNDKYTQNIKECFEYFDKDKDQLLNFEEFQELCLGLFEPFLSPKSAFIIVDVQNDFINGSLALKNCPANEDGAEVIPMINSILTRFIEGDYKFKSVVYTYDWHPKDHISFFDNIHCRRISQHSPISLIDVKPLDKVIFETDSGEETDQILWPKHCVENSDGAALHKDLMVIDGAYHLKKGIFSNVDSYSAFGLNHPKAVTDLNNHLKSHDINFLFVCGLATDVCVAQTALDGLSLGYRVALVKDACRGVNQENIDNVQCKLIENHAIIISSAMISHILNGRVKPVQLAVSLIKRITNK
Sbjct:    1 MIDIHENDINEFYDRLSKFIGMPGEVTVVDILEWFNAIKFSSLNLEDFSELFNIFFNGCNDKYTQNIKECFEYFDKDKDQLLNFEEFQELCLGLFEPFLSPKSAFIIVDVQNDFINGSLALKNCPANEDGAEVIPMINSILTRFIEGDYKFKSVVYTYDWHPKDHISFFDNIHCRRISQHSPISLIDVKPLDKVIFETDSGEETDQILWPKHCVENSDGAALHKDLMVIDGAYHLKKGIFSNVDSYSAFGLNHPKAVTDLNNHLKSHDINFLFVCGLATDVCVAQTALDGLSLGYRVALVKDACRGVNQENIDNVQCKLIENHAIIISSAMISHILNGRVKPVQLAVSLIKRITNK 356
The following BLAST results are available for this feature:
BLAST of Pyrazinamidase/nicotinamidase, putative vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Pyrazinamidase/nicotinamidase, putative vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 4
Match NameE-valueIdentityDescription
pnc-28.130e-3535.22Pyrazinamidase and NiCotinamidase [Source:UniProt... [more]
pnc-11.145e-3031.51Pyrazinamidase and NiCotinamidase [Source:UniProt... [more]
pnc-11.145e-3031.51Pyrazinamidase and NiCotinamidase [Source:UniProt... [more]
pnc-11.897e-3031.51Pyrazinamidase and NiCotinamidase [Source:UniProt... [more]
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BLAST of Pyrazinamidase/nicotinamidase, putative vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Naam7.018e-5637.70gene:FBgn0051216 transcript:FBtr0083786[more]
Naam7.018e-5637.70gene:FBgn0051216 transcript:FBtr0335415[more]
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BLAST of Pyrazinamidase/nicotinamidase, putative vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Pyrazinamidase/nicotinamidase, putative vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Pyrazinamidase/nicotinamidase, putative vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Pyrazinamidase/nicotinamidase, putative vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P21369|PNCA_ECOLI8.432e-2936.65Nicotinamidase OS=Escherichia coli (strain K12) OX... [more]
sp|P53184|PNC1_YEAST7.704e-2233.04Nicotinamidase OS=Saccharomyces cerevisiae (strain... [more]
sp|Q9USS0|PNC1_SCHPO1.261e-2034.27Nicotinamidase OS=Schizosaccharomyces pombe (strai... [more]
sp|I6XD65|PNCA_MYCTU1.234e-829.44Nicotinamidase/pyrazinamidase OS=Mycobacterium tub... [more]
sp|O32091|PNCA_BACSU1.843e-830.95Uncharacterized isochorismatase family protein Pnc... [more]
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BLAST of Pyrazinamidase/nicotinamidase, putative vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A2P6KV785.994e-6842.72PncA OS=Nephila clavipes OX=2585209 GN=NCL1_27050 ... [more]
T1JPC26.427e-6743.06Uncharacterized protein OS=Strigamia maritima OX=1... [more]
A0A131Y2P31.732e-6543.90Putative pyrazinamidase/nicotinamidase pnc1 OS=Ixo... [more]
A0A2R5LCU73.201e-6540.00Putative pyrazinamidase/nicotinamidase pnc1 (Fragm... [more]
V5IIR06.187e-6440.82Putative pyrazinamidase/nicotinamidase pnc1 OS=Ixo... [more]
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BLAST of Pyrazinamidase/nicotinamidase, putative vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Pyrazinamidase/nicotinamidase, putative vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Pyrazinamidase/nicotinamidase, putative vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1
Match NameE-valueIdentityDescription
PNC11.110e-2333.04Nicotinamidase that converts nicotinamide to nicot... [more]
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BLAST of Pyrazinamidase/nicotinamidase, putative vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO444021.641e-4542.27Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Pyrazinamidase/nicotinamidase, putative vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Pyrazinamidase/nicotinamidase, putative vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000005583.10.000e+099.72SMESG000005583.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30028895 ID=SMED30028895|Name=Pyrazinamidase/nicotinamidase, putative|organism=Schmidtea mediterranea sexual|type=transcript|length=3038bp
GAATTCATGCTGCGCATCATCTGGCGGCGCACGATTTTCTTCTTTTACGG
CAGTCAAAGTGTTAAAGATCTAAATATTTAAATAAATACATACGCACCGC
CAGATAAAGCGGTCTAGTGCGGGCCACAGATGGTACGCTACGGCGTGAAC
GTGTTAAGTAGAGATGATAGTGCAAAAATGTATTTGAGACAGACCTATAA
TTTATAGTCGCGTCACTGATTGTAACAAATTTAGTAGTTTCCATTCCAGA
AAAAATCAAACATTAAAACTGATGTGGCACAAATGTTTGGAAAGTGAATG
TTGAATCAGATTTTGTTGACCTCGGCTTTGTCATTCCTTGCATAAAATCA
TAGGTCATTTTAGTTTTGTTATTTTTAAATTAAGTTTCATTTATTTTTAC
TTTTGTTTATTCACACAAGTATTGGACTTTTATATGTTGAACATTTAAAT
ACACGTATCTTTTGAATTTTAACTCGGTGTGATATGAGCTGTACTAGGTT
AAAAATTGCTTGCCTAGATTATCAGTTGTCATTCAAAATTCTCTCAAACA
CCATTTTCATTATCTACTTGATTTAGATCTGATACTTTAAAGTTTGTTTA
AATGGATGTTGAATTTCTAATTGCATCGCATATTTAGAAGAGATTCTTTT
TGTGTGATGAAATTTGCTTCTGTGGTCAAATTGGTCTGTCATCTGCTTTA
ATACGGTTTTATGGCTGTATTGTCAGGAAATGTAAGTGATAATTGAACCT
GTTTTAATGATTTATCTTGTATAATGATTTATTCTGTATCTTAAGTTATT
GAAGGAATAGAAACAGCGTGAATTGGTTATAACTATTTCCAGGACATTTG
ATATAGTGACAATAAAATATCGCCATTTAAATAAAAATATATTAATATTT
CACAAAGCCTACTATTCTGTATTTTATACAGAGGTTCTCAAACTTTTATT
ATCACTGAAGATTTTACACATTTACAATTTTCTTCAACCAAGAACTTCAT
TGTAGATAGAATTTCTTAAGGTTATCTCTAATAGCTTGTTTTAGAAGATG
GATAATCAGTTTTTTCCGACAGTATTCCTTGAATTCTTTAATACAGTTTT
GATCAAGTGGCTAGAGAATTGATGTTTTGTCTTCAGATAAAAATATTATC
ACAAGTACTTAAATCTAATAGGTCGTTTTGTAGCATTTACACAGGCAGTA
TCATCAATCATTCTCTAGAATTCTGTCACATATGAGTATTTTTCCATAGT
TCTTTCTCGTCAACATTATATGTATTTTTGAGGCATAAATTAGCTCGGAT
ATTTTTCCTTTAAATCTGGAAATGAAATCAGAAACCGCTGAATGATTCAC
AGATAATTTTCTCACTCAAATTCTTAATGACCAAATTCTGTATCTCTTTT
TGAAATTTCCAGTGAACCAGAATTACCGGAAAACTTCACCTCGATGTCGA
GAGAACAATGAAATTCAAAGGTCTATAATATTAAAGTTCCGTTATTAACA
AAGTTCGATTTCATGCAGTTTTAATATTGTGCACCGTAGACAATCTTATT
ATTTTAATTTTATTGCATTGCCTTAAGTCATTCGCGGCAAATTCTCCAAT
CTTCTATTTAGAAGTTTCATTACGAGCTACTGATTTTTCAGAGATACGTT
GATTGTCTGCCGGTTTTTGATGTTTTGCTTTTTTTATAATTGAACCATTT
TTCGGGGGGATTGCTTTAATTTAACTCTACAATTTTATTTCACAATAAAA
ATATTTTGTTCTTTAGTCATATAATGATTGATATTCATGAAAATGATATC
AATGAATTTTATGATCGGCTTTCAAAATTTATTGGAGTGCCAGGAGAGGT
GACTGTTGTGGACATTTTAGAATGGTTCAATGCAATAAAATTTTCAAGCT
TGAATTTAGAAGATTTTTCAGAACTGTTTAATATTTTTTTCAATGGCTGT
AACGATAAATATACTCAAAATATCAAAGAATGCTTTGAATATTTTGATAA
AGATAAGGATCAACTTTTGAATTTTGAAGAATTTCAAGAATTATGCCTTG
GATTATTTGAACCTTTTTTAAGTCCAAAATCAGCATTTATTATTGTCGAT
GTTCAAAATGACTTTATAAACGGATCATTAGCTTTAAAAAATTGTCCAGC
AAATGAAGATGGCGCCGAAGTTATACCAATGATCAATTCTATATTAACTA
GATTTATTGAGGGAGATTACAAATTCAAATCTGTCGTTTATACTTACGAC
TGGCACCCTAAAGATCACATATCGTTTTTTGATAATATCCATTGTCGAAG
AATTAGTCAACATTCACCGATTAGCCTAATTGACGTGAAGCCGCTGGATA
AAGTTATTTTTGAAACAGACAGTGGTGAAGAGACTGATCAAATTTTGTGG
CCAAAACATTGTGTTGAAAACTCTGATGGAGCCGCTTTACACAAGGATTT
GATGGTAATTGATGGTGCATATCATTTGAAAAAGGGGATATTTTCCAACG
TTGACAGTTATTCAGCATTTGGGCTTAATCATCCAAAAGCCGTAACAGAT
TTAAACAACCACTTAAAAAGTCATGATATTAATTTTCTTTTTGTTTGTGG
TTTAGCTACAGATGTTTGTGTTGCTCAAACTGCATTGGATGGGTTAAGTC
TTGGCTACCGGGTTGCTCTGGTGAAAGACGCGTGCAGGGGTGTCAACCAA
GAAAATATAGATAATGTCCAATGTAAACTTATTGAAAATCATGCTATTAT
AATTTCATCAGCAATGATTTCGCATATCTTAAATGGAAGGGTTAAACCCG
TTCAGCTGGCTGTGTCCTTAATTAAACGAATTACCAATAAATAATTTAAT
TTTATATAGCACGTTATTTCAAGATGTTTTATTTATAGTTTTAACTATAT
GAAAAATCTATTTGATATATTATTTAGTACATTAGTTCATCTATTAATGA
TATATTTAACTGATTATTCTGAGACTTTATAAGCTTTCTTGTTTTGTCGT
TTTTCGCTTTATTAAATTATACTTTTGTTGATTTTAAA
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protein sequence of SMED30028895-orf-1

>SMED30028895-orf-1 ID=SMED30028895-orf-1|Name=SMED30028895-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=357bp
MIDIHENDINEFYDRLSKFIGVPGEVTVVDILEWFNAIKFSSLNLEDFSE
LFNIFFNGCNDKYTQNIKECFEYFDKDKDQLLNFEEFQELCLGLFEPFLS
PKSAFIIVDVQNDFINGSLALKNCPANEDGAEVIPMINSILTRFIEGDYK
FKSVVYTYDWHPKDHISFFDNIHCRRISQHSPISLIDVKPLDKVIFETDS
GEETDQILWPKHCVENSDGAALHKDLMVIDGAYHLKKGIFSNVDSYSAFG
LNHPKAVTDLNNHLKSHDINFLFVCGLATDVCVAQTALDGLSLGYRVALV
KDACRGVNQENIDNVQCKLIENHAIIISSAMISHILNGRVKPVQLAVSLI
KRITNK*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:0008936nicotinamidase activity
GO:0016787hydrolase activity
GO:0003824catalytic activity
GO:0005509calcium ion binding
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
Vocabulary: INTERPRO
TermDefinition
IPR036380Isochorismatase-like_sf
IPR011992EF-hand-dom_pair
IPR000868Isochorismatase-like
IPR018247EF_Hand_1_Ca_BS
IPR002048EF_hand_dom
Vocabulary: biological process
TermDefinition
GO:0008152metabolic process
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:1.10.238.10coord: 23..96
e-value: 1.7E-5
score: 27.1
NoneNo IPR availablePANTHERPTHR11080:SF2LD05707Pcoord: 85..344
NoneNo IPR availablePANTHERPTHR11080PYRAZINAMIDASE/NICOTINAMIDASEcoord: 85..344
IPR000868Isochorismatase-likePFAMPF00857Isochorismatasecoord: 103..327
e-value: 4.0E-27
score: 95.5
IPR036380Isochorismatase-like superfamilyGENE3DG3DSA:3.40.50.850coord: 103..328
e-value: 2.5E-56
score: 193.1
IPR036380Isochorismatase-like superfamilySUPERFAMILYSSF52499Isochorismatase-like hydrolasescoord: 99..338
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 75..87
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 62..97
score: 11.919
IPR011992EF-hand domain pairSUPERFAMILYSSF47473EF-handcoord: 8..112