SMED30028854

Overview
NameSMED30028854
Smed IDSMED30028854
Length (bp)845
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30028854 (SMED30028854) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30028854 (SMED30028854) in cells (dots) of each of the 12 neoblast clusters.

 

back to top


 

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30028854 (SMED30028854) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30028854 (SMED30028854) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

back to top


 

Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

back to top
Anatomical Expression

PAGE et. al., 2020




SMED30028854

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 11

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
gutSMED30028854 Contig1674GPL15192PMID:23079596
Forsthoefel et al., 2012
whole organism asexual adult colorimetric in situ hybridization evidence
intestinal phagocyteSMED30028854 Contig1674GPL15192PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
Smed sexual biotypeSMED30028854SMESG000010546.1 SMESG000010469.1 SMESG000010468.1 SMESG000010467.1 SMESG000010464.1 SMESG000010218.1 SMESG000010465.1 Contig16092uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30028854SMESG000010546.1 SMESG000010469.1 SMESG000010468.1 SMESG000010467.1 SMESG000010464.1 SMESG000010218.1 SMESG000010465.1 Contig16092newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
pharynxSMED30028854SMESG000019970.1 SMESG000019968.1 SMESG000019959.1 SMESG000019761.1 SMESG000010368.1 SMESG000010348.1 SMESG000010322.1 SMESG000019776.1 SMESG000010338.1 SMESG000010333.1 SMESG000010321.1 SMESG000043477.1 SMESG000010347.1 SMESG000010327.1 SMESG000043474.1 SMESG000049178.1 SMESG000010579.1 SMESG000010549.1 SMESG000010548.1 SMESG000010546.1 SMESG000010468.1 SMESG000010467.1 SMESG000010222.1 SMESG000010218.1 SMESG000010217.1 SMESG000019966.1 SMESG000010464.1 SMESG000010469.1 SMESG000019962.1 dd_Smed_v6_593_1dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30028854SMESG000019970.1 SMESG000019968.1 SMESG000019959.1 SMESG000019761.1 SMESG000010368.1 SMESG000010348.1 SMESG000010322.1 SMESG000019776.1 SMESG000010338.1 SMESG000010333.1 SMESG000010321.1 SMESG000043477.1 SMESG000010347.1 SMESG000010327.1 SMESG000043474.1 SMESG000049178.1 SMESG000010579.1 SMESG000010549.1 SMESG000010548.1 SMESG000010546.1 SMESG000010468.1 SMESG000010467.1 SMESG000010222.1 SMESG000010218.1 SMESG000010217.1 SMESG000019966.1 SMESG000010464.1 SMESG000010469.1 SMESG000019962.1 dd_Smed_v6_593_1dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
intestinal phagocyteSMED30028854SMESG000019970.1 SMESG000019968.1 SMESG000019959.1 SMESG000019761.1 SMESG000010368.1 SMESG000010348.1 SMESG000010322.1 SMESG000019776.1 SMESG000010338.1 SMESG000010333.1 SMESG000010321.1 SMESG000043477.1 SMESG000010347.1 SMESG000010327.1 SMESG000043474.1 SMESG000049178.1 SMESG000010579.1 SMESG000010549.1 SMESG000010548.1 SMESG000010546.1 SMESG000010468.1 SMESG000010467.1 SMESG000010222.1 SMESG000010218.1 SMESG000010217.1 SMESG000019966.1 SMESG000010464.1 SMESG000010469.1 SMESG000019962.1 dd_Smed_v6_593_1dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30028854SMESG000019970.1 SMESG000019968.1 SMESG000019959.1 SMESG000019761.1 SMESG000010368.1 SMESG000010348.1 SMESG000010322.1 SMESG000019776.1 SMESG000010338.1 SMESG000010333.1 SMESG000010321.1 SMESG000043477.1 SMESG000010347.1 SMESG000010327.1 SMESG000043474.1 SMESG000049178.1 SMESG000010579.1 SMESG000010549.1 SMESG000010548.1 SMESG000010546.1 SMESG000010468.1 SMESG000010467.1 SMESG000010222.1 SMESG000010218.1 SMESG000010217.1 SMESG000019966.1 SMESG000010464.1 SMESG000010469.1 SMESG000019962.1 dd_Smed_v6_593_1dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30028854SMESG000019970.1 SMESG000019968.1 SMESG000019959.1 SMESG000019761.1 SMESG000010368.1 SMESG000010348.1 SMESG000010322.1 SMESG000019776.1 SMESG000010338.1 SMESG000010333.1 SMESG000010321.1 SMESG000043477.1 SMESG000010347.1 SMESG000010327.1 SMESG000043474.1 SMESG000049178.1 SMESG000010579.1 SMESG000010549.1 SMESG000010548.1 SMESG000010546.1 SMESG000010468.1 SMESG000010467.1 SMESG000010222.1 SMESG000010218.1 SMESG000010217.1 SMESG000019966.1 SMESG000010464.1 SMESG000010469.1 SMESG000019962.1 dd_Smed_v6_593_1dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30028854SMESG000010604.1 dd_Smed_v4_8471_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
ciliated epithelial cellSMED30028854 dd_Smed_v4_3945_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30028854 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.002674:19957..22381 +2631
Homology
BLAST of SMED30028854 vs. Ensembl Xenopus
Match: ANK1 (ankyrin 1 [Source:NCBI gene;Acc:613080])

HSP 1 Score: 49.6766 bits (117), Expect = 3.895e-6
Identity = 62/227 (27.31%), Postives = 94/227 (41.41%), Query Frame = -3
Query:   82 LIDRSIDIDQTGFMGLTGLHCAVIRGMVNVCAQLVNRGADCMRSVEIINQYSGKENA---PAIPFAQSIISLTCTENALTL---------SMKFHGTLFPCFVDRLDLRSSNVHDGNNNSL--LHLAVKSLQSNTCISLLNAGAQVNSINNFGNSPLLEMFCE----------TQLAVNDDNLNKL-------------IDITNILFGNGAQHNSISFNKITIFQLA 651
            L++R    +  G  GLT LH AV    +N+   L+N+GA    +    N Y+    A    ++  A++++    T NA +L         + + HG +    V  L  + +NVH GN +SL  LHLA +    N    L++  A V +    G +P L + C            Q   N + + KL              DI  +L  NGA  N  S N IT   +A
Sbjct:  513 LLEREAHPNSAGKTGLTPLHVAVYHNHLNIVRLLLNKGASPHCTAR--NGYTPLHMAVKQRSVDTARALLQFGATCNAQSLQGVTPLHLAAQEGHGDM----VTLLLSKQANVHLGNKSSLTPLHLASQEGHLNIAEVLVSHDASVAAETRMGFAP-LHVACHYGNIKVVKFLLQHQANVNAVTKLGFTPLHQAAQQGHTDIVTLLLKNGACPNQASMNGITPLAIA 732          
BLAST of SMED30028854 vs. Ensembl Xenopus
Match: ANK1 (ankyrin 1 [Source:NCBI gene;Acc:613080])

HSP 1 Score: 49.2914 bits (116), Expect = 5.105e-6
Identity = 62/227 (27.31%), Postives = 94/227 (41.41%), Query Frame = -3
Query:   82 LIDRSIDIDQTGFMGLTGLHCAVIRGMVNVCAQLVNRGADCMRSVEIINQYSGKENA---PAIPFAQSIISLTCTENALTL---------SMKFHGTLFPCFVDRLDLRSSNVHDGNNNSL--LHLAVKSLQSNTCISLLNAGAQVNSINNFGNSPLLEMFCE----------TQLAVNDDNLNKL-------------IDITNILFGNGAQHNSISFNKITIFQLA 651
            L++R    +  G  GLT LH AV    +N+   L+N+GA    +    N Y+    A    ++  A++++    T NA +L         + + HG +    V  L  + +NVH GN +SL  LHLA +    N    L++  A V +    G +P L + C            Q   N + + KL              DI  +L  NGA  N  S N IT   +A
Sbjct:  588 LLEREAHPNSAGKTGLTPLHVAVYHNHLNIVRLLLNKGASPHCTAR--NGYTPLHMAVKQRSVDTARALLQFGATCNAQSLQGVTPLHLAAQEGHGDM----VTLLLSKQANVHLGNKSSLTPLHLASQEGHLNIAEVLVSHDASVAAETRMGFAP-LHVACHYGNIKVVKFLLQHQANVNAVTKLGFTPLHQAAQQGHTDIVTLLLKNGACPNQASMNGITPLAIA 807          
BLAST of SMED30028854 vs. Ensembl Xenopus
Match: ANK1 (ankyrin 1 [Source:NCBI gene;Acc:613080])

HSP 1 Score: 49.2914 bits (116), Expect = 5.108e-6
Identity = 62/227 (27.31%), Postives = 94/227 (41.41%), Query Frame = -3
Query:   82 LIDRSIDIDQTGFMGLTGLHCAVIRGMVNVCAQLVNRGADCMRSVEIINQYSGKENA---PAIPFAQSIISLTCTENALTL---------SMKFHGTLFPCFVDRLDLRSSNVHDGNNNSL--LHLAVKSLQSNTCISLLNAGAQVNSINNFGNSPLLEMFCE----------TQLAVNDDNLNKL-------------IDITNILFGNGAQHNSISFNKITIFQLA 651
            L++R    +  G  GLT LH AV    +N+   L+N+GA    +    N Y+    A    ++  A++++    T NA +L         + + HG +    V  L  + +NVH GN +SL  LHLA +    N    L++  A V +    G +P L + C            Q   N + + KL              DI  +L  NGA  N  S N IT   +A
Sbjct:  588 LLEREAHPNSAGKTGLTPLHVAVYHNHLNIVRLLLNKGASPHCTAR--NGYTPLHMAVKQRSVDTARALLQFGATCNAQSLQGVTPLHLAAQEGHGDM----VTLLLSKQANVHLGNKSSLTPLHLASQEGHLNIAEVLVSHDASVAAETRMGFAP-LHVACHYGNIKVVKFLLQHQANVNAVTKLGFTPLHQAAQQGHTDIVTLLLKNGACPNQASMNGITPLAIA 807          
BLAST of SMED30028854 vs. Ensembl Xenopus
Match: ANK1 (ankyrin 1 [Source:NCBI gene;Acc:613080])

HSP 1 Score: 48.9062 bits (115), Expect = 5.989e-6
Identity = 62/227 (27.31%), Postives = 94/227 (41.41%), Query Frame = -3
Query:   82 LIDRSIDIDQTGFMGLTGLHCAVIRGMVNVCAQLVNRGADCMRSVEIINQYSGKENA---PAIPFAQSIISLTCTENALTL---------SMKFHGTLFPCFVDRLDLRSSNVHDGNNNSL--LHLAVKSLQSNTCISLLNAGAQVNSINNFGNSPLLEMFCE----------TQLAVNDDNLNKL-------------IDITNILFGNGAQHNSISFNKITIFQLA 651
            L++R    +  G  GLT LH AV    +N+   L+N+GA    +    N Y+    A    ++  A++++    T NA +L         + + HG +    V  L  + +NVH GN +SL  LHLA +    N    L++  A V +    G +P L + C            Q   N + + KL              DI  +L  NGA  N  S N IT   +A
Sbjct:  551 LLEREAHPNSAGKTGLTPLHVAVYHNHLNIVRLLLNKGASPHCTAR--NGYTPLHMAVKQRSVDTARALLQFGATCNAQSLQGVTPLHLAAQEGHGDM----VTLLLSKQANVHLGNKSSLTPLHLASQEGHLNIAEVLVSHDASVAAETRMGFAP-LHVACHYGNIKVVKFLLQHQANVNAVTKLGFTPLHQAAQQGHTDIVTLLLKNGACPNQASMNGITPLAIA 770          
BLAST of SMED30028854 vs. Ensembl Xenopus
Match: ANK1 (ankyrin 1 [Source:NCBI gene;Acc:613080])

HSP 1 Score: 48.9062 bits (115), Expect = 7.446e-6
Identity = 62/227 (27.31%), Postives = 94/227 (41.41%), Query Frame = -3
Query:   82 LIDRSIDIDQTGFMGLTGLHCAVIRGMVNVCAQLVNRGADCMRSVEIINQYSGKENA---PAIPFAQSIISLTCTENALTL---------SMKFHGTLFPCFVDRLDLRSSNVHDGNNNSL--LHLAVKSLQSNTCISLLNAGAQVNSINNFGNSPLLEMFCE----------TQLAVNDDNLNKL-------------IDITNILFGNGAQHNSISFNKITIFQLA 651
            L++R    +  G  GLT LH AV    +N+   L+N+GA    +    N Y+    A    ++  A++++    T NA +L         + + HG +    V  L  + +NVH GN +SL  LHLA +    N    L++  A V +    G +P L + C            Q   N + + KL              DI  +L  NGA  N  S N IT   +A
Sbjct:  515 LLEREAHPNSAGKTGLTPLHVAVYHNHLNIVRLLLNKGASPHCTAR--NGYTPLHMAVKQRSVDTARALLQFGATCNAQSLQGVTPLHLAAQEGHGDM----VTLLLSKQANVHLGNKSSLTPLHLASQEGHLNIAEVLVSHDASVAAETRMGFAP-LHVACHYGNIKVVKFLLQHQANVNAVTKLGFTPLHQAAQQGHTDIVTLLLKNGACPNQASMNGITPLAIA 734          
BLAST of SMED30028854 vs. Planmine SMEST
Match: SMESG000049178.1 (SMESG000049178.1)

HSP 1 Score: 387.497 bits (994), Expect = 3.001e-130
Identity = 197/256 (76.95%), Postives = 211/256 (82.42%), Query Frame = -3
Query:   76 SLAYSLHRRNCIGVLQKYQLNVNTRNKVNQLSNRY*Y*CIGII*YSETPLDEAIASRLDDNCGYLIDRSIDIDQTGFMGLTGLHCAVIRGMVNVCAQLVNRGADCMRSVEIINQYSGKENAPAIPFAQSIISLTCTENALTLSMKFHGTLFPCFVDRLDLRSSNVHDGNNNSLLHLAVKSLQSNTCISLLNAGAQVNSINNFGNSPLLEMFCETQLAVNDDNLNKLIDITNILFGNGAQHNSISFNKITIFQLA*N 843
            +LAYSLH+R CI VLQ+YQL+VNTRNK                 YSETPL EAIAS L+D CGYLI+RSIDIDQTGFMGLTGLHCAVIRGMVNVCAQLVNRGADCMRSVEIINQYSGKENAPAIPFAQSIISLTCTENALTLSMKFHGTLFPCFVDRLDLRSSNVHDGNNNSLLHLAVK L+ + C SLLNAGA VN+INNF NSPLLEM  + QL  N++NLNK I+IT IL G GA  N+   N IT FQLA N
Sbjct:  245 ALAYSLHQRKCIRVLQQYQLDVNTRNK-----------------YSETPLHEAIASELEDKCGYLINRSIDIDQTGFMGLTGLHCAVIRGMVNVCAQLVNRGADCMRSVEIINQYSGKENAPAIPFAQSIISLTCTENALTLSMKFHGTLFPCFVDRLDLRSSNVHDGNNNSLLHLAVKCLKPDICSSLLNAGAHVNNINNFENSPLLEMLNDAQLVANENNLNKSIEITKILIGKGADTNAQLSNGITAFQLAAN 483          
BLAST of SMED30028854 vs. Planmine SMEST
Match: SMESG000010579.1 (SMESG000010579.1)

HSP 1 Score: 391.734 bits (1005), Expect = 1.158e-129
Identity = 198/256 (77.34%), Postives = 213/256 (83.20%), Query Frame = -3
Query:   76 SLAYSLHRRNCIGVLQKYQLNVNTRNKVNQLSNRY*Y*CIGII*YSETPLDEAIASRLDDNCGYLIDRSIDIDQTGFMGLTGLHCAVIRGMVNVCAQLVNRGADCMRSVEIINQYSGKENAPAIPFAQSIISLTCTENALTLSMKFHGTLFPCFVDRLDLRSSNVHDGNNNSLLHLAVKSLQSNTCISLLNAGAQVNSINNFGNSPLLEMFCETQLAVNDDNLNKLIDITNILFGNGAQHNSISFNKITIFQLA*N 843
            +LAYSLH+R CI VLQ+YQL+VNTRNK                 YSETPL EAIAS L+D CGYLIDRSIDIDQTGFMGLTGLHCAVIRGMVNVCAQLVNRGADCMRSVEIINQYSGKENAP+IPFAQSIISLTCTENALTLSMKFHGTLFPCFVDRLDLRSSNVHDGNNNSLLHLAVK L+ + C SLLNAGA VN++NNFGNSPLLEM  + QL  N++NLNK I+IT IL GNGA  N+   N IT FQLA N
Sbjct:  425 ALAYSLHQRKCIRVLQQYQLDVNTRNK-----------------YSETPLHEAIASELEDKCGYLIDRSIDIDQTGFMGLTGLHCAVIRGMVNVCAQLVNRGADCMRSVEIINQYSGKENAPSIPFAQSIISLTCTENALTLSMKFHGTLFPCFVDRLDLRSSNVHDGNNNSLLHLAVKCLKQDICSSLLNAGAHVNTLNNFGNSPLLEMLNDAQLVANENNLNKSIEITKILIGNGADINAQLSNGITAFQLAAN 663          
BLAST of SMED30028854 vs. Planmine SMEST
Match: SMESG000049178.1 (SMESG000049178.1)

HSP 1 Score: 386.341 bits (991), Expect = 1.363e-128
Identity = 197/256 (76.95%), Postives = 211/256 (82.42%), Query Frame = -3
Query:   76 SLAYSLHRRNCIGVLQKYQLNVNTRNKVNQLSNRY*Y*CIGII*YSETPLDEAIASRLDDNCGYLIDRSIDIDQTGFMGLTGLHCAVIRGMVNVCAQLVNRGADCMRSVEIINQYSGKENAPAIPFAQSIISLTCTENALTLSMKFHGTLFPCFVDRLDLRSSNVHDGNNNSLLHLAVKSLQSNTCISLLNAGAQVNSINNFGNSPLLEMFCETQLAVNDDNLNKLIDITNILFGNGAQHNSISFNKITIFQLA*N 843
            +LAYSLH+R CI VLQ+YQL+VNTRNK                 YSETPL EAIAS L+D CGYLI+RSIDIDQTGFMGLTGLHCAVIRGMVNVCAQLVNRGADCMRSVEIINQYSGKENAPAIPFAQSIISLTCTENALTLSMKFHGTLFPCFVDRLDLRSSNVHDGNNNSLLHLAVK L+ + C SLLNAGA VN+INNF NSPLLEM  + QL  N++NLNK I+IT IL G GA  N+   N IT FQLA N
Sbjct:  357 ALAYSLHQRKCIRVLQQYQLDVNTRNK-----------------YSETPLHEAIASELEDKCGYLINRSIDIDQTGFMGLTGLHCAVIRGMVNVCAQLVNRGADCMRSVEIINQYSGKENAPAIPFAQSIISLTCTENALTLSMKFHGTLFPCFVDRLDLRSSNVHDGNNNSLLHLAVKCLKPDICSSLLNAGAHVNNINNFENSPLLEMLNDAQLVANENNLNKSIEITKILIGKGADTNAQLSNGITAFQLAAN 595          
BLAST of SMED30028854 vs. Planmine SMEST
Match: SMESG000010549.1 (SMESG000010549.1)

HSP 1 Score: 387.882 bits (995), Expect = 8.027e-128
Identity = 197/256 (76.95%), Postives = 213/256 (83.20%), Query Frame = -3
Query:   76 SLAYSLHRRNCIGVLQKYQLNVNTRNKVNQLSNRY*Y*CIGII*YSETPLDEAIASRLDDNCGYLIDRSIDIDQTGFMGLTGLHCAVIRGMVNVCAQLVNRGADCMRSVEIINQYSGKENAPAIPFAQSIISLTCTENALTLSMKFHGTLFPCFVDRLDLRSSNVHDGNNNSLLHLAVKSLQSNTCISLLNAGAQVNSINNFGNSPLLEMFCETQLAVNDDNLNKLIDITNILFGNGAQHNSISFNKITIFQLA*N 843
            +LAYSLH+R CI VLQ+YQL+VNTRNK                 YSETPL EAIAS L+D CGYLI+RSIDIDQTGFMGLTGLHCAVIRGMVNVCAQLVNRGADCMRSVEIINQYSGKENAPAIPFAQSIISLTCTENALTLSMKFHGTLFPCFVDRLDLRSSNVHDGNNNSLLHLAVK L+ + C SLLNAGAQVN++NNFGNSPLLEM  + QL  N +NLNK I+IT IL G+GA  ++   N IT FQLA N
Sbjct:  462 ALAYSLHQRKCIRVLQQYQLDVNTRNK-----------------YSETPLHEAIASELEDKCGYLINRSIDIDQTGFMGLTGLHCAVIRGMVNVCAQLVNRGADCMRSVEIINQYSGKENAPAIPFAQSIISLTCTENALTLSMKFHGTLFPCFVDRLDLRSSNVHDGNNNSLLHLAVKCLKPDICSSLLNAGAQVNTLNNFGNSPLLEMLNDAQLVANKNNLNKSIEITKILIGSGADVSAQLSNGITAFQLAAN 700          
BLAST of SMED30028854 vs. Planmine SMEST
Match: SMESG000010549.1 (SMESG000010549.1)

HSP 1 Score: 387.882 bits (995), Expect = 1.603e-127
Identity = 197/256 (76.95%), Postives = 213/256 (83.20%), Query Frame = -3
Query:   76 SLAYSLHRRNCIGVLQKYQLNVNTRNKVNQLSNRY*Y*CIGII*YSETPLDEAIASRLDDNCGYLIDRSIDIDQTGFMGLTGLHCAVIRGMVNVCAQLVNRGADCMRSVEIINQYSGKENAPAIPFAQSIISLTCTENALTLSMKFHGTLFPCFVDRLDLRSSNVHDGNNNSLLHLAVKSLQSNTCISLLNAGAQVNSINNFGNSPLLEMFCETQLAVNDDNLNKLIDITNILFGNGAQHNSISFNKITIFQLA*N 843
            +LAYSLH+R CI VLQ+YQL+VNTRNK                 YSETPL EAIAS L+D CGYLI+RSIDIDQTGFMGLTGLHCAVIRGMVNVCAQLVNRGADCMRSVEIINQYSGKENAPAIPFAQSIISLTCTENALTLSMKFHGTLFPCFVDRLDLRSSNVHDGNNNSLLHLAVK L+ + C SLLNAGAQVN++NNFGNSPLLEM  + QL  N +NLNK I+IT IL G+GA  ++   N IT FQLA N
Sbjct:  494 ALAYSLHQRKCIRVLQQYQLDVNTRNK-----------------YSETPLHEAIASELEDKCGYLINRSIDIDQTGFMGLTGLHCAVIRGMVNVCAQLVNRGADCMRSVEIINQYSGKENAPAIPFAQSIISLTCTENALTLSMKFHGTLFPCFVDRLDLRSSNVHDGNNNSLLHLAVKCLKPDICSSLLNAGAQVNTLNNFGNSPLLEMLNDAQLVANKNNLNKSIEITKILIGSGADVSAQLSNGITAFQLAAN 732          
The following BLAST results are available for this feature:
BLAST of SMED30028854 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30028854 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30028854 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30028854 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30028854 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ANK13.895e-627.31ankyrin 1 [Source:NCBI gene;Acc:613080][more]
ANK15.105e-627.31ankyrin 1 [Source:NCBI gene;Acc:613080][more]
ANK15.108e-627.31ankyrin 1 [Source:NCBI gene;Acc:613080][more]
ANK15.989e-627.31ankyrin 1 [Source:NCBI gene;Acc:613080][more]
ANK17.446e-627.31ankyrin 1 [Source:NCBI gene;Acc:613080][more]
back to top
BLAST of SMED30028854 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30028854 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30028854 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30028854 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30028854 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30028854 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30028854 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30028854 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30028854 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000049178.13.001e-13076.95SMESG000049178.1[more]
SMESG000010579.11.158e-12977.34SMESG000010579.1[more]
SMESG000049178.11.363e-12876.95SMESG000049178.1[more]
SMESG000010549.18.027e-12876.95SMESG000010549.1[more]
SMESG000010549.11.603e-12776.95SMESG000010549.1[more]
back to top
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30028854 ID=SMED30028854|Name=SMED30028854|organism=Schmidtea mediterranea sexual|type=transcript|length=845bp
CGCCATCTTTCGTATTGCTTTTTACAGATTCATATAATTCATAACAACAA
TAAAATAAAAATAGCAATTACTCTCATTCTATGCCAATTGAAATATGGTG
ATTTTATTGAATGATATCGAGTTGTGTTGAGCTCCATTTCCAAACAAAAT
ATTTGTGATATCGATTAATTTATTTAAGTTGTCATCATTAACAGCTAGTT
GTGTCTCACAAAACATCTCAAGCAATGGAGAGTTACCGAAATTGTTGATA
CTGTTGACCTGTGCTCCAGCGTTAAGCAGTGAAATACATGTATTTGATTG
GAGGGATTTCACTGCCAGATGAAGCAAGGAATTGTTGTTCCCATCATGAA
CATTTGAACTTCTTAAATCGAGTCGGTCAACAAAACATGGAAAAAGCGTC
CCATGAAATTTCATTGACAAAGTTAGCGCGTTTTCAGTACAAGTCAGTGA
AATTATTGATTGTGCAAATGGAATTGCGGGAGCATTTTCTTTTCCAGAAT
ATTGATTAATTATCTCAACAGATCTCATACAATCTGCACCCCGATTCACT
AATTGTGCACAAACATTTACCATGCCCCGAATCACTGCACAATGTAGACC
AGTCAATCCCATAAATCCAGTCTGGTCAATATCTATTGATCGATCAATCA
AATATCCGCAATTGTCATCCAATCTACTCGCAATTGCTTCATCCAATGGA
GTTTCCGAATACTAAATAATGCCAATACATCAATATTAATATCGATTACT
TAACTGATTTACTTTGTTTCTTGTGTTTACATTGAGTTGATATTTCTGCA
AAACTCCTATGCAATTCCTTCGATGTAGAGAATAAGCTAATGATA
back to top

protein sequence of SMED30028854-orf-1

>SMED30028854-orf-1 ID=SMED30028854-orf-1|Name=SMED30028854-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=178bp
MGLTGLHCAVIRGMVNVCAQLVNRGADCMRSVEIINQYSGKENAPAIPFA
QSIISLTCTENALTLSMKFHGTLFPCFVDRLDLRSSNVHDGNNNSLLHLA
VKSLQSNTCISLLNAGAQVNSINNFGNSPLLEMFCETQLAVNDDNLNKLI
DITNILFGNGAQHNSISFNKITIFQLA*
back to top
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000026gut
PLANA:0000034epidermis
PLANA:0000070intestinal phagocyte
PLANA:0000106ciliated epithelial cell
PLANA:0000429neoblast
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR036770Ankyrin_rpt-contain_sf
IPR002110Ankyrin_rpt
IPR020683Ankyrin_rpt-contain_dom
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002110Ankyrin repeatSMARTSM00248ANK_2acoord: 1..30
e-value: 0.26
score: 20.4
coord: 92..121
e-value: 3.4
score: 16.7
IPR002110Ankyrin repeatPROSITEPS50088ANK_REPEATcoord: 1..27
score: 8.656
IPR002110Ankyrin repeatPROSITEPS50088ANK_REPEATcoord: 92..124
score: 9.11
IPR020683Ankyrin repeat-containing domainPROSITEPS50297ANK_REP_REGIONcoord: 92..130
score: 10.816
IPR020683Ankyrin repeat-containing domainPROSITEPS50297ANK_REP_REGIONcoord: 1..27
score: 8.641
IPR036770Ankyrin repeat-containing domain superfamilySUPERFAMILYSSF48403Ankyrin repeatcoord: 2..131