hemicentin-1 isoform X2

Overview
Namehemicentin-1 isoform X2
Smed IDSMED30028839
Length (bp)3766
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of hemicentin-1 isoform X2 (SMED30028839) t-SNE clustered cells

Violin plots show distribution of expression levels for hemicentin-1 isoform X2 (SMED30028839) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of hemicentin-1 isoform X2 (SMED30028839) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for hemicentin-1 isoform X2 (SMED30028839) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30028839

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30028839h1SMcG0010434 SmedASXL_017195SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
protonephridiaSMED30028839h1SMcG0010434 dd_Smed_v4_13903_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30028839h1SMcG0010434 dd_Smed_v4_13903_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30028839h1SMcG0010434 dd_Smed_v6_13903_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
ciliated epithelial cellSMED30028839h1SMcG0010434 dd_Smed_v4_13903_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of hemicentin-1 isoform X2 vs. Ensembl Human
Match: MXRA5 (matrix remodeling associated 5 [Source:HGNC Symbol;Acc:HGNC:7539])

HSP 1 Score: 107.071 bits (266), Expect = 1.764e-22
Identity = 180/793 (22.70%), Postives = 310/793 (39.09%), Query Frame = 3
Query:  363 LTIHNISSDNSGYYVCRVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQWVIMQGLQIIDS-------------SKFIAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLIHIDSNYRV----PKNSN------YSINCRVTSNPPPEFIKWYKLSNMTDSSAVLISDDAKL----NFILIDRSST----GMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETKDIVSDMET---------FQVINSES--------RHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARW-RYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXN-----IRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEV-VFKSQPDP----PYDLRVTDFEWDSVTINWKPGFNGGLTQNYEILVISENF-----GTQNVPFYSDKNENTLTITGLYPSTPYEIRIYAVNSIGRS 2549
            L I  +   + G Y+C  + L   + ++ L  VTV+ P + ++ H+ + + V+   + IA++C ++ G P P+IS +I  D  +   + +S      T+ E   +S+    +  +RG   C A N AG  +    L      P I       ++     +I + C   A P  +++WV+  G QI  S             + +I  + P   G   C A N +GS + TVQLNV R       +N R+    P+ ++        ++C  + +P P  +       M D+   L S D+++    N  L+ +S T    G Y CV +N         K  +    + + V  KP + +        +  G    + C       G P P + W   +   + S M++         + V N+ +        R  G Y C AEN++GK   R    V V  AP+ I       V + Y D+   ++  CE +G+P+ +  W    N+ IP SS+ +              QI+   T+ +       Q + RS  +G Y C   N  G      ++ +   P             R+IAA  ++   +I C+AE I T  + W    E +VLP        +   +T +               L I+ L   D     C+A N+ G  ++  ++ V +    P    P   ++T     ++++N     +   T    ++ +  N      G Q   FY  K +  L I+GL        R  A N+ G +
Sbjct: 1913 LVIRKVQVQDRGQYMCTASNLHGLDRMVVLLSVTVQQP-QILASHY-QDVTVYL-GDTIAMEC-LAKGTPAPQIS-WIFPDRRV--WQTVSPVEGRITLHENRTLSIKEASF-SDRGVYKCVASNAAGADSLAIRLHVAALPPVIHQEKLENISLPPGLSIHIHCTAKAAPLPSVRWVLGDGTQIRPSQFLHGNLFVFPNGTLYIRNLAPKDSGRYECVAANLVGSARRTVQLNVQR-----AAANARITGTSPRRTDVRYGGTLKLDCSASGDPWPRILWRLPSKRMIDA---LFSFDSRIKVFANGTLVVKSVTDKDAGDYLCVARN---------KVGDDYVVLKVDVVMKPAKIEHKEENDHKVFYGGDLKVDCVAT----GLPNPEISWSLPDGSLVNSFMQSDDSGGRTKRYVVFNNGTLYFNEVGMREEGDYTCFAENQVGKDEMR--VRVKVVTAPATIRNKTYLAVQVPYGDV---VTVACEAKGEPMPKVTWLSPTNKVIPTSSEKY--------------QIYQDGTLLI-------QKAQRSD-SGNYTCLVRNSAGEDRKTVWIHVNVQPPKINGNPNPITTVREIAAGGSRK--LIDCKAEGIPTPRVLW-AFPEGVVLP-----APYYGNRITVH-----------GNGSLDIRSLRKSDSVQLVCMARNEGGEARLILQLTVLEPMEKPIFHDPISEKITAMAGHTISLN----CSAAGTPTPSLVWVLPNGTDLQSGQQLQRFY-HKADGMLHISGLSSVDAGAYRCVARNAAGHT 2625          

HSP 2 Score: 69.707 bits (169), Expect = 4.792e-11
Identity = 158/711 (22.22%), Postives = 261/711 (36.71%), Query Frame = 3
Query:  330 ESQITISHESR-LTIHNISSDNSGYYVCRVTLLTTSEHI-ISLAVVTVKTPPKSISFHFNKSINVFQEQEF-IAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSN---FPNSTIAEVSAISLWRPHYLEN-----RGFVMCTAINDAGVLTKNTSLLNIKYKP---KIKPFVPNTVNAMINQTIDLSCEVYANPAATIQWVIMQG------------LQIIDSSKFIAKIRPDMD-GPLICEAKNYLGSTKETVQLNVLRDP--LIHIDSN-YRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMTDSSAV---LISDDAK-------------LNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKI---ISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETKDIVSDMETFQVIN--------SESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKN-----RKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSS-QDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIA---ASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQG 2264
            E +IT+ HE+R L+I   S  + G Y C  +    ++ + I L V  +  PP     H  K  N+       I I C+     P P +   +     I+  + +  N   FPN T+            Y+ N      G   C A N  G   + T  LN++      +I    P   +     T+ L C    +P   I W +               +++  +   + K   D D G  +C A+N +G     ++++V+  P  + H + N ++V    +  ++C  T  P PE I W    ++ D S V   + SDD+              L F  +     G Y C  +N      GK    +  +     VT     P  + NK    + +  G+   + C    ++ GEP P + W+    K I +  E +Q+          ++   +G Y CL  N  G+   R+T  + VN  P  I  + N     R++    S         C+  G P     W +    + P+     +I  H N S            +D+         SLR S + +  C A N  G A L   L +     ++  I H  I+    ++  + + + C A    T  + W       VLP  +   S    ++ ++  + D    G+    L I  L + D   Y CVA N  G
Sbjct: 1999 EGRITL-HENRTLSIKEASFSDRGVYKCVASNAAGADSLAIRLHVAAL--PP---VIHQEKLENISLPPGLSIHIHCTAKAA-PLPSVRWVLGDGTQIRPSQFLHGNLFVFPNGTL------------YIRNLAPKDSGRYECVAANLVGS-ARRTVQLNVQRAAANARITGTSPRRTDVRYGGTLKLDCSASGDPWPRILWRLPSKRMIDALFSFDSRIKVFANGTLVVKSVTDKDAGDYLCVARNKVGDDYVVLKVDVVMKPAKIEHKEENDHKVFYGGDLKVDCVATGLPNPE-ISW----SLPDGSLVNSFMQSDDSGGRTKRYVVFNNGTLYFNEVGMREEGDYTCFAEN----QVGK----DEMRVRVKVVT----APATIRNKTYLAVQVPYGDVVTVAC----EAKGEPMPKVTWLSPTNKVIPTSSEKYQIYQDGTLLIQKAQRSDSGNYTCLVRNSAGE--DRKTVWIHVNVQPPKINGNPNPITTVREIAAGGS----RKLIDCKAEGIPTPRVLWAFPEGVVLPAPYYGNRITVHGNGS------------LDIR--------SLRKSDSVQLVCMARNEGGEARL--ILQLTVLEPMEKPIFHDPISEKITAMAGHTISLNCSAAGTPTPSLVW-------VLPNGTDLQS--GQQLQRFYHKAD----GM----LHISGLSSVDAGAYRCVARNAAG 2623          

HSP 3 Score: 53.1434 bits (126), Expect = 4.655e-6
Identity = 111/560 (19.82%), Postives = 201/560 (35.89%), Query Frame = 3
Query:  774 NIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQWVI------------MQGLQIIDSSKF-IAKIRPDMDGPLICEAKNYLGSTKETVQLNV-LRDPLIHIDSNYR---VPKNSNYSINCRVTSNPPPEFI-------KWYKLSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKI--ISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETKDI-----------VSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAP--SIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRK--IAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPF--------QSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQP 2306
            ++  KP+I    P TV+          CE    P   + W              +Q  +++ +    I K++    G  +C A N  G  +  V L+V ++ P I + S+Y+   V      ++ C     P P+          W  +S +     + + ++  L+      S  G+Y CV  N           A    ++ + +      P I   K+  IS+  G    I C+        P P+++W+  +   I           V    T  + N   + +G+Y+C+A N +G   +R T  + V  A   + IT    R+  + Y     ++   C   G P     WR  ++++  +   F         + RI ++F   T+ + S T            G Y C A N+ G    DY ++     +    I H++           + + C A  +    I W   +  +V  F        ++K+Y +F                 L  NE+ +++ E D    Y+C A N  G  ++   V   + P
Sbjct: 1848 SLGEKPQILTKSPQTVSVTAETDTVFPCEATGKPKPFVTWTKVSTGALMTPNTRIQRFEVLKNGTLVIRKVQVQDRGQYMCTASNLHGLDRMVVLLSVTVQQPQI-LASHYQDVTVYLGDTIAMECLAKGTPAPQISWIFPDRRVWQTVSPV--EGRITLHENRTLSIKEASFSDRGVYKCVASN-----------AAGADSLAIRLHVAALPPVIHQEKLENISLPPGLSIHIHCTAK----AAPLPSVRWVLGDGTQIRPSQFLHGNLFVFPNGTLYIRNLAPKDSGRYECVAANLVGS--ARRTVQLNVQRAAANARITGTSPRRTDVRYGG---TLKLDCSASGDPWPRILWRLPSKRMIDALFSF---------DSRI-KVFANGTLVVKSVTDKDA--------GDYLCVARNKVGD---DYVVLKVDVVMKPAKIEHKEENDHKVFYGGDLKVDCVATGLPNPEISWSLPDGSLVNSFMQSDDSGGRTKRYVVFNNGT-------------LYFNEVGMRE-EGD----YTCFAENQVGKDEMRVRVKVVTAP 2345          
BLAST of hemicentin-1 isoform X2 vs. Ensembl Human
Match: ROBO2 (roundabout guidance receptor 2 [Source:HGNC Symbol;Acc:HGNC:10250])

HSP 1 Score: 106.686 bits (265), Expect = 2.084e-22
Identity = 146/630 (23.17%), Postives = 238/630 (37.78%), Query Frame = 3
Query:  810 PNTVNAMINQTIDLSCEVYANPAATIQWVI-------------MQGLQIIDSSKFIAKI------RPDMDGPLICEAKNYLG---STKETVQLNVLRDPLIHIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMTDSSAVLIS-DDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFI-IKCSTDRKSPGEPFPTLKWIDVETKDI------VSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTK-LTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHG--IASLDYFLXXXXXXXXXXNIRHRKIAASLN-QNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVV---------------FKSQPDPPYDLRVTDFEWDSVTINWKPGFNGGLTQN-YEILVISENFGTQNVPFYSDKNENTLTITGLYPSTPYEIRIYAVNSIGRS 2549
            P+ V     +   L+C+    P  TI+W                  + +   S F  +I      +PD +G  +C A+NYLG   S   ++++ +LRD      ++  V       + C+     P   I W K     D     IS    KL      +S  GMY CV  N+     G+R    +E T+F       + P  L   I  ++  E+ +  +C       G+P PT++W   +  D+      + D  T ++  + S   G Y C+AEN +GK     +A +TV   P  + + +++ V        ++++F CE +G P     W+         SQ+    +     N R S   T  LTI          T+++ S  G Y C+A    G  +A     +           I       +L      +++C+A       I W K  E    P +  + +I E                     L IK+L   D   Y+CVA++  G  + ++  V                   P PP   +VTD   +SVT++W+PG  G L  + Y I   S++         +       T+ GL P+T Y   + A+N  G S
Sbjct:   37 PSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPD-EGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNM----VGERDSDPAELTVF-------ERPTFLRRPINQVVLEEEAVEFRCQVQ----GDPQPTVRW-KKDDADLPRGRYDIKDDYTLRIKKTMSTDEGTYMCIAENRVGK--MEASATLTVRAPPQFVVRPRDQIVAQG-----RTVTFPCETKGNPQPAVFWQKEG------SQNLLFPNQPQQPNSRCSVSPTGDLTI----------TNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPANQTLAVDGTALLKCKATGDPLPVISWLK--EGFTFPGRDPRATIQEQ------------------GTLQIKNLRISDTGTYTCVATSSSG--ETSWSAVLDVTESGATISKNYDLSDLPGPPSKPQVTDVTKNSVTLSWQPGTPGTLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLS 604          

HSP 2 Score: 62.3882 bits (150), Expect = 6.201e-9
Identity = 101/467 (21.63%), Postives = 172/467 (36.83%), Query Frame = 3
Query:  360 RLTIHNISSDNSGYYVC--RVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVK---DNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMI--NQTIDLSCEVYANPAATIQWV-----IMQGLQII--DSSKFIAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLIHIDSNYR-VPKNSNYSINCRVTSNPPPEFIKWYKLSNMT---------DSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWI-------DVETKDIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLP 1667
            R+     S  + G YVC  R  L        SL V  ++       F  N +  V    E   ++C    G P P I     K   D+  +R+ I       S   +  A            G   C   N  G    + + L +  +P    F+   +N ++   + ++  C+V  +P  T++W      + +G   I  D +  I K     +G  +C A+N +G  + +  L V   P   +    + V +    +  C    NP P    W K  +            +S   +S    L    I RS  G Y C    +  S   K   A  E T  LT    P   Q   N+ +++      ++KC    K+ G+P P + W+         + +  + +  T Q+ N      G Y C+A +  G+  +  +A++ V E+ + I+++ +       SDLP
Sbjct:   94 RIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRD-----DFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDA------------GMYTCVGTNMVGERDSDPAELTVFERPT---FLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKTMSTDEGTYMCIAENRVGKMEASATLTVRAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVF-WQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAK---AQLEVTDVLTDRPPPIILQGPANQTLAV--DGTALLKC----KATGDPLPVISWLKEGFTFPGRDPRATIQEQGTLQIKNLRISDTGTYTCVATSSSGE--TSWSAVLDVTESGATISKNYD------LSDLP 522          
BLAST of hemicentin-1 isoform X2 vs. Ensembl Human
Match: ROBO2 (roundabout guidance receptor 2 [Source:HGNC Symbol;Acc:HGNC:10250])

HSP 1 Score: 105.916 bits (263), Expect = 3.069e-22
Identity = 146/630 (23.17%), Postives = 238/630 (37.78%), Query Frame = 3
Query:  810 PNTVNAMINQTIDLSCEVYANPAATIQWVI-------------MQGLQIIDSSKFIAKI------RPDMDGPLICEAKNYLG---STKETVQLNVLRDPLIHIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMTDSSAVLIS-DDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFI-IKCSTDRKSPGEPFPTLKWIDVETKDI------VSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTK-LTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHG--IASLDYFLXXXXXXXXXXNIRHRKIAASLN-QNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVV---------------FKSQPDPPYDLRVTDFEWDSVTINWKPGFNGGLTQN-YEILVISENFGTQNVPFYSDKNENTLTITGLYPSTPYEIRIYAVNSIGRS 2549
            P+ V     +   L+C+    P  TI+W                  + +   S F  +I      +PD +G  +C A+NYLG   S   ++++ +LRD      ++  V       + C+     P   I W K     D     IS    KL      +S  GMY CV  N+     G+R    +E T+F       + P  L   I  ++  E+ +  +C       G+P PT++W   +  D+      + D  T ++  + S   G Y C+AEN +GK     +A +TV   P  + + +++ V        ++++F CE +G P     W+         SQ+    +     N R S   T  LTI          T+++ S  G Y C+A    G  +A     +           I       +L      +++C+A       I W K  E    P +  + +I E                     L IK+L   D   Y+CVA++  G  + ++  V                   P PP   +VTD   +SVT++W+PG  G L  + Y I   S++         +       T+ GL P+T Y   + A+N  G S
Sbjct:   53 PSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPD-EGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNM----VGERDSDPAELTVF-------ERPTFLRRPINQVVLEEEAVEFRCQVQ----GDPQPTVRW-KKDDADLPRGRYDIKDDYTLRIKKTMSTDEGTYMCIAENRVGK--MEASATLTVRAPPQFVVRPRDQIVAQG-----RTVTFPCETKGNPQPAVFWQKEG------SQNLLFPNQPQQPNSRCSVSPTGDLTI----------TNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPANQTLAVDGTALLKCKATGDPLPVISWLK--EGFTFPGRDPRATIQEQ------------------GTLQIKNLRISDTGTYTCVATSSSG--ETSWSAVLDVTESGATISKNYDLSDLPGPPSKPQVTDVTKNSVTLSWQPGTPGTLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLS 620          

HSP 2 Score: 62.3882 bits (150), Expect = 6.596e-9
Identity = 103/474 (21.73%), Postives = 175/474 (36.92%), Query Frame = 3
Query:  360 RLTIHNISSDNSGYYVC--RVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDN----------AIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMI--NQTIDLSCEVYANPAATIQWV-----IMQGLQII--DSSKFIAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLIHIDSNYR-VPKNSNYSINCRVTSNPPPEFIKWYKLSNMT---------DSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWI-------DVETKDIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLP 1667
            R+     S  + G YVC  R  L        SL V  ++       F  N +  V    E   ++C    G P P I  Y  KD           +I+  K++ SN   S                 + G   C   N  G    + + L +  +P    F+   +N ++   + ++  C+V  +P  T++W      + +G   I  D +  I K     +G  +C A+N +G  + +  L V   P   +    + V +    +  C    NP P    W K  +            +S   +S    L    I RS  G Y C    +  S   K   A  E T  LT    P   Q   N+ +++      ++KC    K+ G+P P + W+         + +  + +  T Q+ N      G Y C+A +  G+  +  +A++ V E+ + I+++ +       SDLP
Sbjct:  110 RIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRD-----DFRQNPTDVVVAAGEPAILECQPPRGHPEPTI--YWKKDKVRIDDKEERISIRGGKLMISNTRKS-----------------DAGMYTCVGTNMVGERDSDPAELTVFERPT---FLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKTMSTDEGTYMCIAENRVGKMEASATLTVRAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVF-WQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAK---AQLEVTDVLTDRPPPIILQGPANQTLAV--DGTALLKC----KATGDPLPVISWLKEGFTFPGRDPRATIQEQGTLQIKNLRISDTGTYTCVATSSSGE--TSWSAVLDVTESGATISKNYD------LSDLP 538          
BLAST of hemicentin-1 isoform X2 vs. Ensembl Human
Match: ROBO2 (roundabout guidance receptor 2 [Source:HGNC Symbol;Acc:HGNC:10250])

HSP 1 Score: 105.916 bits (263), Expect = 3.170e-22
Identity = 146/630 (23.17%), Postives = 238/630 (37.78%), Query Frame = 3
Query:  810 PNTVNAMINQTIDLSCEVYANPAATIQWVI-------------MQGLQIIDSSKFIAKI------RPDMDGPLICEAKNYLG---STKETVQLNVLRDPLIHIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMTDSSAVLIS-DDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFI-IKCSTDRKSPGEPFPTLKWIDVETKDI------VSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTK-LTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHG--IASLDYFLXXXXXXXXXXNIRHRKIAASLN-QNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVV---------------FKSQPDPPYDLRVTDFEWDSVTINWKPGFNGGLTQN-YEILVISENFGTQNVPFYSDKNENTLTITGLYPSTPYEIRIYAVNSIGRS 2549
            P+ V     +   L+C+    P  TI+W                  + +   S F  +I      +PD +G  +C A+NYLG   S   ++++ +LRD      ++  V       + C+     P   I W K     D     IS    KL      +S  GMY CV  N+     G+R    +E T+F       + P  L   I  ++  E+ +  +C       G+P PT++W   +  D+      + D  T ++  + S   G Y C+AEN +GK     +A +TV   P  + + +++ V        ++++F CE +G P     W+         SQ+    +     N R S   T  LTI          T+++ S  G Y C+A    G  +A     +           I       +L      +++C+A       I W K  E    P +  + +I E                     L IK+L   D   Y+CVA++  G  + ++  V                   P PP   +VTD   +SVT++W+PG  G L  + Y I   S++         +       T+ GL P+T Y   + A+N  G S
Sbjct:   37 PSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPD-EGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNM----VGERDSDPAELTVF-------ERPTFLRRPINQVVLEEEAVEFRCQVQ----GDPQPTVRW-KKDDADLPRGRYDIKDDYTLRIKKTMSTDEGTYMCIAENRVGK--MEASATLTVRAPPQFVVRPRDQIVAQG-----RTVTFPCETKGNPQPAVFWQK------EGSQNLLFPNQPQQPNSRCSVSPTGDLTI----------TNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPANQTLAVDGTALLKCKATGDPLPVISWLK--EGFTFPGRDPRATIQEQ------------------GTLQIKNLRISDTGTYTCVATSSSG--ETSWSAVLDVTESGATISKNYDLSDLPGPPSKPQVTDVTKNSVTLSWQPGTPGTLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLS 604          

HSP 2 Score: 62.003 bits (149), Expect = 7.350e-9
Identity = 103/474 (21.73%), Postives = 175/474 (36.92%), Query Frame = 3
Query:  360 RLTIHNISSDNSGYYVC--RVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDN----------AIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMI--NQTIDLSCEVYANPAATIQWV-----IMQGLQII--DSSKFIAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLIHIDSNYR-VPKNSNYSINCRVTSNPPPEFIKWYKLSNMT---------DSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWI-------DVETKDIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLP 1667
            R+     S  + G YVC  R  L        SL V  ++       F  N +  V    E   ++C    G P P I  Y  KD           +I+  K++ SN   S                 + G   C   N  G    + + L +  +P    F+   +N ++   + ++  C+V  +P  T++W      + +G   I  D +  I K     +G  +C A+N +G  + +  L V   P   +    + V +    +  C    NP P    W K  +            +S   +S    L    I RS  G Y C    +  S   K   A  E T  LT    P   Q   N+ +++      ++KC    K+ G+P P + W+         + +  + +  T Q+ N      G Y C+A +  G+  +  +A++ V E+ + I+++ +       SDLP
Sbjct:   94 RIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRD-----DFRQNPTDVVVAAGEPAILECQPPRGHPEPTI--YWKKDKVRIDDKEERISIRGGKLMISNTRKS-----------------DAGMYTCVGTNMVGERDSDPAELTVFERPT---FLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKTMSTDEGTYMCIAENRVGKMEASATLTVRAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVF-WQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAK---AQLEVTDVLTDRPPPIILQGPANQTLAV--DGTALLKC----KATGDPLPVISWLKEGFTFPGRDPRATIQEQGTLQIKNLRISDTGTYTCVATSSSGE--TSWSAVLDVTESGATISKNYD------LSDLP 522          
BLAST of hemicentin-1 isoform X2 vs. Ensembl Human
Match: ROBO1 (roundabout guidance receptor 1 [Source:HGNC Symbol;Acc:HGNC:10249])

HSP 1 Score: 105.145 bits (261), Expect = 5.265e-22
Identity = 157/666 (23.57%), Postives = 264/666 (39.64%), Query Frame = 3
Query:  705 NRGFVMCTAINDAGVLTKNTSLLNIK-YKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQWVIMQGLQIIDSSK---------------FIAKI------RPDMDGPLICEAKNYLG---STKETVQLNVLRDPLIHIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYK-LSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWI--DVE---TKDIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRY-GNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVV-----MIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQG-------IKKVNFEVVFKSQ--------PDPPYDLRVTDFEWDSVTINWKPGFNGGLT-QNYEILVISENFGTQNVPFYSDKNENTLTITGLYPSTPYEIRIYAVNSIG 2543
            + G  + T+ ND   L    S L  + + P+I    P+ +     +   L+C+    P  TI+W   +G + +++ K               F  +I      RPD +G  +C A+NYLG   S   ++++ +LRD      S+  V       + C+     P   I W K  S + D    +     KL      +S  G Y CV  N+     G+R+   +E    LTV  +P   +   N  +++    +F  KC    ++ G+P PT++W   D E   ++  + D  T ++    +   G Y C+AEN +GK  +  +A +TV E P  + + +++ V L      ++++F CE  G P     WR  G++ +      F  +  ++ S   +SQ    LTI          T+++ S  G Y C+  N  G      +L +      +     R+    +NQ V      ++ C A       I W K  + +++  Q  +    E  V                  L I+  +  D   Y+C+AS   G       I+   F V  +          P  P    VTD   ++VT++W+P  N G T  +Y I   S   G+       +    T  I GL P+  Y   + A N+ G
Sbjct:   39 DHGTPIPTSDNDDNSLGYTGSRLRQEDFPPRIVEH-PSDLIVSKGEPATLNCKAEGRPTPTIEW--YKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPD-EGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNM----VGERESEVAE----LTVLERPSFVKRPSNLAVTVDDSAEF--KC----EARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGK--AEASATLTVQEPPHFVVKPRDQVVALG-----RTVTFQCEATGNPQPAIFWRREGSQNL-----LFSYQPPQSSSRFSVSQT-GDLTI----------TNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQ--GPVNQTVAVDGTFVLSCVATGSPVPTILWRK--DGVLVSTQDSRIKQLENGV------------------LQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETSAIKGLKPNAIYLFLVRAANAYG 641          
BLAST of hemicentin-1 isoform X2 vs. Ensembl Celegans
Match: igcm-1 (ImmunoGlobulin-like Cell adhesion Molecule family [Source:UniProtKB/TrEMBL;Acc:Q9TXI8])

HSP 1 Score: 129.028 bits (323), Expect = 8.007e-30
Identity = 178/764 (23.30%), Postives = 297/764 (38.87%), Query Frame = 3
Query:  396 GYYVCRVTLLTTSE-HIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDA--GVLTKN--TSLLNIKYKPKIKPFVPNTVNAM-----INQTIDLSCEVYANPAA-TIQWVIMQGLQIIDSSKFIAKIRP-----DMDGPLICEAKNYLGSTKETVQLNVLRDPLIHIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQN-IQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETKDIVS----DMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNER-ISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRK-----IAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQPDPPYDLRVTDFEWDSVTINWKPGFNGGLTQNY--EILVISENFGT--QNVP--------FYSDK-------NENTLTITGLYPSTPYEIRIYAVNSIGRS 2549
            G Y C V     S+    +LA V V  PP         S +V  E +F+  +C   GG P P  + + + ++ I    I ++ F +       ++  +R    ++   V C   N A  G  TK   +S LN+ YKP +  FV  T N         + ++L+C   +NP A + +W       I    ++  KI P      M G   C + N LG     +++ V   P I++  +    +  +  I C V++ P    IKW   +           + ++L    I ++ +G Y C+  N + +      +      T  + V  KP + QI+  +  ++  GE   + C  +    G P  +  W    +  I        ++F+V N++   NG Y C A N++G+     T ++TV E   I +     ++F S     Q     CE +G P    +W      +  S     I      S  + + + F   T+   + T +    L+ S  G + C ADN       D         + K    +RK     +AA+   +  +IRC+  +       W   +  I    +++++S     +T    E +          L I+ ++  D+  Y C A+N  G   V  E+   S P  P DL+      +SV + W P F+GG  Q Y  E   I    G    N P           D+       ++N    TGL P + Y +RI AV+  G S
Sbjct:  115 GTYHCNVIGTDDSDVQYSALATVVVLVPPGDPIISTTPSESVI-EGDFMTAKCVSVGGSPQPTFT-WTLPNDTIASSAIFTTQFRD---GATESLLHFRVTSSDDGKSVQCDVTNRAMLGGETKQVKSSQLNVLYKPVV--FVTPTENLTHLSVEEGELVNLTCNSASNPPAHSYEW-----KHIASGERYQGKIWPIRVDSSMSGDFECRSTNELGEGTAVLKMVVQHIPRINVPESISPNEMEDIDILCEVSAVPEVIDIKWVGPNGFKQ-------NGSRLTITSISKAQSGNYTCMATNFLTVYGHSGSQQRMGTGTTIVDVKRKPGQAQIVSARQ-NVDVGETIKLMCQAE--DAGNPSASYTWASPSSGGIFGLEGHTEKSFEVRNAQLSDNGVYSCKAYNDLGEG-KIGTVMITVIEKARISSPLATERIFTSGE---QGKILECEAQGYPSPVIKWLKDGRPVKQS-----IYASSATSESKCLPEDFCTQTV---ASTLTLAGPLKWSDKGNFTCVADNGSDKTDKDSSSWTIVRVLHKPVFLNRKFPEKSLAAADIGSQAIIRCKVSARPEPEFHWMFRDSEIS---ENERHSFHTVGITGKPDEYEQL--------LQIESVQESDYGEYVCRATNGNGGDHVVIELRKTSPPALPLDLQKLSATSNSVLLGWVPQFDGGADQTYVLETRKIDPFTGEIDGNAPVSKLNINAVQQDQEIDGTLVSKNIFNFTGLTPLSTYNLRIQAVSEKGES 833          
BLAST of hemicentin-1 isoform X2 vs. Ensembl Celegans
Match: him-4 (High Incidence of Males (Increased X chromosome loss) [Source:UniProtKB/TrEMBL;Acc:A4UVK0])

HSP 1 Score: 104.375 bits (259), Expect = 4.599e-22
Identity = 157/706 (22.24%), Postives = 278/706 (39.38%), Query Frame = 3
Query:  306 KPCVVPDSESQIT-ISHESRLTIHNISSDNSGYYVCRVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLE-NRGFVMCTAINDAGVLTKNTSLLNIKYKPKI-KPFVPNTVNAMINQTIDLSCEVYANPAATIQWVIMQGLQIIDSSKFIA-----------KIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLI---HIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMTDSSAVLISDDA--KLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVE-TKD----------------IVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQ--HKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAE-SISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQP 2306
            +P  +   +++IT +S++ RLTI N++ ++ G Y CRV            A V V  PP  I    +K+     E   + + C  +G  P P I+ +  KD     ++ I+   PN    E++   L      E + G   C A N AG + ++ ++ N+   PKI K  +P+   +  N+ + +SC VYA P A I W  ++  + + S KF+            K+R        C A N  G+ K   ++++L  P     +I     V   +  +++C    +P P  I W K  N  + +   +  DA  +L     + S  G Y C+  N    + G     N+ + +          P I G +  ++   E F  +   D  S G        +DVE  KD                I S  +    +++    +G+Y C+  N  G+  +R+     VN+ PSI  +    N +  + Y      +   C   G P  +  W + ++ + P S  +++ ++        SQ+                        G Y CEA N  G A  D+ + +  PP  +   + R+   +   + +++ C AE S+    + W+  +E +     + KY+  E                     L + +++ DD   Y C A N+ GI K  F+++    P
Sbjct: 2231 QPIDLEAEDARITRLSNDRRLTILNVTENDEGQYSCRVKNDAGENSFDFKATVLV--PPTIIMLDKDKNKTAV-EHSTVTLSCPATGK-PEPDITWF--KDGEAIHIENIADIIPN---GELNGNQLKITRIKEGDAGKYTCEADNSAGSVEQDVNV-NVITIPKIEKDGIPSDYESQQNERVVISCPVYARPPAKITW--LKAGKPLQSDKFVKTSANGQKLYLFKLRETDSSKYTCIATNEAGTDKRDFKVSMLVAPSFDEPNIVRRITVNSGNPSTLHCPAKGSPSPT-ITWLKDGNAIEPNDRYVFFDAGRQLQISKTEGSDQGRYTCIATN----SVGSDDLENTLEVII--------PPVIDGERREAVAVIEGFSSELFCDSNSTG--------VDVEWQKDGLTINQDTLRGDSFIQIPSSGKKMSFLSARKSDSGRYTCIVRNPAGE--ARKLFDFAVNDPPSISDELSSANIQTIVPY----YPVEINCVVSGSPHPKVYWLFDDKPLEPDSAAYELTNNGETLKIVRSQV---------------------EHAGTYTCEAQNNVGKARKDFLVRVTAPPHFE---KEREEVVARVGDTMLLTCNAESSVPLSSVYWHAHDESVQNGVITSKYAANE-------------------KTLNVTNIQLDDEGFYYCTAVNEAGITKKFFKLIVIETP 2854          

HSP 2 Score: 94.3597 bits (233), Expect = 4.775e-19
Identity = 148/706 (20.96%), Postives = 273/706 (38.67%), Query Frame = 3
Query:  306 KPCVVPDSESQITISHESRLTIHNISSDNSGYYVCRVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLW-RPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEV-YANPAATIQWV-----IMQGLQI-IDSSKFIAKIR---PDMDGPLICEAKNYLGSTKETVQLNVLRDP-----------------------LIHIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSN--MTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVE---TKDIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQP 2306
            K  + PD   +I   +E +L I     +++G Y C    +   +  + +A ++V   P  I       +N+ ++   + +QC ++ GIPPP+I          ++  ++ +   N    +++  +L      +E++G   C A N  G  +++T+L+       +   VP     +  Q + LSC V    P  +I W+     + +G  I I+    + ++R   P  +G   C A +  G++   + + +++ P                       +  ++S + V     ++I C V+  PPP  I WY        +S    ++ D  L     D+S +G+Y C   N    + G     N +KT    +      P   G    +++  + F I C       G+P P + W+  +   T+ +V++  +  + N    H G + C A+N  G      T  +TV+  P+I  +++  K+ L   D    I   C  +  P     W Y  EKI     D ++  H               TI        Q   L ++  G + C+  N  G  SL Y L +   P I   +    +   +    + I C+A  +  +   W K    I L    KK+S+                    +  L I + + +D  NY+CV +N+ G  ++   V  +  P
Sbjct:  741 KDLIKPDDYIKI---NEGQLHIMGAKDEDAGAYSCVGENMAGKD--VQVANLSVGRVPTIIESPHTVRVNIERQ---VTLQC-LAVGIPPPEIEW--------QKGNVLLATLNNPRYTQLADGNLLITDAQIEDQGQFTCIARNTYGQQSQSTTLMVTGLVSPVLGHVPPEEQLIEGQDLTLSCVVVLGTPKPSIVWIKDDKPVEEGPTIKIEGGGSLLRLRGGNPKDEGKYTCIAVSPAGNSTLHINVQLIKKPEFVYKPEGGIVFKPTISGMDEKHVAVVNSTHDVLDGEGFAIPCVVSGTPPP-IITWYLDGRPITPNSRDFTVTADNTLIVRKADKSYSGVYTCQATN----SAGD----NEQKTTIRIMNTPMISP---GQSSFNMVVDDLFTIPCDVY----GDPKPVITWLLDDKPFTEGVVNEDGSLTIPNVNEAHRGTFTCHAQNAAGND--TRTVTLTVHTTPTINAENQ-EKIALQNDD----IVLECPAKALPPPVRLWTYEGEKI-----DSQLIPH---------------TIREDGALVLQNVKLENT--GVFVCQVSNLAGEDSLSYTLTVHEKPKIISEVP--GVVDVVKGFTIEIPCRATGVPEVIRTWNKNG--IDLKMDEKKFSVD------------------NLGTLRIYEADKNDIGNYNCVVTNEAGTSQMTTHVDVQEPP 1362          

HSP 3 Score: 85.8853 bits (211), Expect = 2.296e-16
Identity = 152/735 (20.68%), Postives = 273/735 (37.14%), Query Frame = 3
Query:  165 NATVNSKENLTLECVVDQQNIVYSWEYKPFHIKSNIMSKVLGFCYFEKPCVVPDSESQITISHESRLTIHNISSDNSGYYVC-RVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENR-GFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQWVIMQGLQIIDSSKFIAKI-----RPDM---DGPLICEAKNYLGSTKETVQLNVLRDPLIHIDSNYRVPKNSN-YSINCRVTSNPPPEFIKWYKLSNMTDSSAVLISDDAKLNFILIDRSST----GMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWI------DVETKDIVSDMETFQVINSESRHNGKYKCLAENEIGKS--------FSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNF 2282
            N  V + + LTLEC ++  + V+    + F     +                 D  +Q + S +S+L I   + +++  Y C  V     +E +     VTV TPPK     F  S         + I C   G IPPP+IS ++     ++         P  T  +   +SL   +   N+ G   C A N AG   ++T  + I   P++       +  +  +   + CEV+ NP   + W+        D  +F  K+     R       G   C A N  G ++ T  + VL  P I  +      K  N Y ++C+VT  P P ++ W +     +          + N +L  R++T    G Y+C+  N   +       A+    +F   T++  E         +I++GE   I+C  D    G P PT+ W+      +++   +    +T  ++ ++    G Y C+A N  G S        +++     T+++ P  +   +              I   C   G P     W+ G++ +P  S+      H  ++N            DL      +  S+ +   G+Y+C A N  G  +  Y   +   P      +   +   +  + + + C   S     I W++ ++ + L     +YSI         P+          + +TI   +  D   Y C ASN+ G   ++ 
Sbjct: 2951 NVEVQAGDPLTLECPIEDTSGVHITWSRQFGKDGQL-----------------DMRAQSS-SDKSKLYIMQATPEDADSYSCIAVNDAGGAEAVFQ---VTVNTPPKIFGDSF--STTEIVADTTLEIPCRTEG-IPPPEISWFLDGKPILE--------MPGVTYKQ-GDLSLRIDNIKPNQEGRYTCVAENKAGRAEQDT-YVEISEPPRVV-MASEVMRVVEGRQTTIRCEVFGNPEPVVNWLKDGEPYTSDLLQFSTKLSYLHLRETTLADGGTYTCIATNKAGESQTTTDVEVLVPPRIEDEERVLQGKEGNTYMVHCQVTGRPVP-YVTWKRNGKEIE----------QFNPVLHIRNATRADEGKYSCIASNEAGTAV-----ADFLIDVFTKPTFETHE------TTFNIVEGESAKIECKID----GHPKPTISWLKGGRPFNMDNIILSPRGDTLMILKAQRFDGGLYTCVATNSYGDSEQDFKVNVYTKPYIDETIDQTPKAVAGGE--------------IILKCPVLGNPTPTVTWKRGDDAVPNDSR------HTIVNN-----------YDL------KINSVTTEDAGQYSCIAVNEAGNLTTHYAAEVIGKPTFVR--KGGNLYEVIENDTITMDCGVTSRPLPSISWFRGDKPVYL---YDRYSI--------SPDG---------SHITINKAKLSDGGKYICRASNEAGTSDIDL 3565          

HSP 4 Score: 84.7297 bits (208), Expect = 4.354e-16
Identity = 159/677 (23.49%), Postives = 256/677 (37.81%), Query Frame = 3
Query:  363 LTIHNISSDNSGYYVCRVTLLTTSEHIISLA--VVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQWVIMQGLQIIDSSKFIA--------KIRPDMDGPLICEAKNYLGSTKETVQLNVLRDP-LIHIDSN-YRVPKNSNYSINCRVTSNPPPEFIKWY----------KLSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPK--EPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKW----IDVETKD----IVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFR-----GQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNF 2282
            L I N +  + G Y C    + ++E   ++A  ++ V T P   +F  +++     E E   I+C + G  P P IS ++          II S   ++       + + +    +  G   C A N  G   ++  + N+  KP I   +  T  A+    I L C V  NP  T+ W         DS   I          +  +  G   C A N  G+        V+  P  +    N Y V +N   +++C VTS P P  I W+          + S   D S + I + AKL       S  G Y C   N         +   S+  + L +   PK  +  I+GN +  + +     ++C       G P P + W    +D+   D    +  + ETF + N +    G+Y C A N  GK+ S + +L  +  +P     H  +       D   +I+ TC  +        + +  W          ++D +  D     N  IS    KLTI         Q SL ++  G Y C A NR G ASL++ + I  PP+I  +    +   ++NQ  +M RC         IKW K  + +      +   I E                 +   L I   ++D    +SCVA ND G+K++  
Sbjct: 3264 LHIRNATRADEGKYSC----IASNEAGTAVADFLIDVFTKP---TFETHETTFNIVEGESAKIECKIDGH-PKPTIS-WLKGGRPFNMDNIILSPRGDT-------LMILKAQRFDG-GLYTCVATNSYGDSEQDFKV-NVYTKPYIDETIDQTPKAVAGGEIILKCPVLGNPTPTVTWKRGDDAVPNDSRHTIVNNYDLKINSVTTEDAGQYSCIAVNEAGNLTTHYAAEVIGKPTFVRKGGNLYEVIENDTITMDCGVTSRPLPS-ISWFRGDKPVYLYDRYSISPDGSHITI-NKAKL-------SDGGKYICRASN---------EAGTSDIDLILKILVPPKIDKSNIIGNPLAIVAR--TIYLECPIS----GIPQPDVIWTKNGMDINMTDSRVILAQNNETFGIENVQVTDQGRYTCTATNRGGKA-SHDFSLDVL--SPPEFDIHGTQPTIKREGD---TITLTCPIKLAEDIADQVMDVSW----------TKDSRALDGDLTDNVDISDDGRKLTI--------SQASLENA--GLYTCIALNRAGEASLEFKVEILSPPVIDISRNDVQPQVAVNQPTIM-RCAVTGHPFPSIKWLKNGKEVT---DDENIRIVE-----------------QGQVLQILRTDSDHAGKWSCVAENDAGVKELEM 3851          

HSP 5 Score: 83.9593 bits (206), Expect = 7.278e-16
Identity = 145/704 (20.60%), Postives = 267/704 (37.93%), Query Frame = 3
Query:  291 FCYFEKPCVVPDSESQITISHESRLTIHNISSDNSGYYVCRVTLLTTSEHIISLA----VVTVKTPPKSISFHFNKSIN--VFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKY-----KPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQWVI-------MQGLQIIDSSKFIAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLIHID-SNYRVPKNSNYSINCRV--TSNPPPEFIKWYKLSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKI--ISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETKDIV---------SDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQP 2306
            +  F+   + PDS +    ++   L I     +++G Y C       +++ +  A    +V V  PP     HF K     V +  + + + C+    +P   +  +   D +++   I S    N     V+ I L      ++ GF  CTA+N+AG+  K   L+ I+      + K+ P +       + + + L C     P  TI ++           + II S+  I   + +++G   C A+N  G +++ + + VL  P +  +  N  V      ++ C +  TS     + + +      D  A   SD +KL  +         Y+C+  N           A   + +F      P  P+I G+      I+      I C T+    G P P + W  ++ K I+          D+ + ++ N +    G+Y C+AEN+ G++   +   V ++E P ++   +  +V        +  +  CE  G P     W    E   P + D             + Q  TKL+  L+ R    +T+L  +  G Y C A N+ G +     + +  PP I+      ++      N  M+ CQ       ++ W +  + I                       + F P L      I++    D   YSC+ASN+ G    +F +   ++P
Sbjct: 2701 YWLFDDKPLEPDSAAYELTNNGETLKIVRSQVEHAGTYTCE------AQNNVGKARKDFLVRVTAPP-----HFEKEREEVVARVGDTMLLTCNAESSVPLSSVYWH-AHDESVQNGVITSKYAANEKTLNVTNIQL------DDEGFYYCTAVNEAGITKKFFKLIVIETPYFLDQQKLYPII-------LGKRLTLDCSATGTPPPTILFMKDGKRLNESDEVDIIGSTLVIDNPQKEVEGRYTCIAENKAGRSEKDMMVEVLLPPKLSKEWINVEVQAGDPLTLECPIEDTSGVHITWSRQFGKDGQLDMRAQSSSDKSKLYIMQATPEDADSYSCIAVN----------DAGGAEAVFQVTVNTP--PKIFGDSFSTTEIVADTTLEIPCRTE----GIPPPEISWF-LDGKPILEMPGVTYKQGDL-SLRIDNIKPNQEGRYTCVAENKAGRA--EQDTYVEISEPPRVVMASEVMRVVEG-----RQTTIRCEVFGNPEPVVNWLKDGE---PYTSD-------------LLQFSTKLSY-LHLR----ETTL--ADGGTYTCIATNKAGESQTTTDVEVLVPPRIED---EERVLQGKEGNTYMVHCQVTGRPVPYVTWKRNGKEI-----------------------EQFNPVLH-----IRNATRADEGKYSCIASNEAGTAVADFLIDVFTKP 3300          

HSP 6 Score: 80.1073 bits (196), Expect = 1.096e-14
Identity = 122/562 (21.71%), Postives = 225/562 (40.04%), Query Frame = 3
Query:  342 TISHESRLTIHNISSDNSGYYVCRVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQWVIMQGLQI-IDSSKFIAKIRPDMDGPLI-------------CEAKNYLGSTKETVQLNVLRDPLIH---IDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSN--MTDSSAVLISDDA-KLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWI----------DVETKDIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRY---GNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIA 1928
            TI  +  L + N+  +N+G +VC+V+ L   + +     +TV   PK IS      ++V +    I I C  +G   P  I  +    N I  LK+    F   ++  +  + ++      + G   C   N+AG  ++ T+ ++++  P I P       A++   ++L C V A+P A++ W   +G+ I  D+  ++     + DG L+             C+A N  G  +  +Q+ V+  P I    + +   + ++  +S+ C V SNP P+ I WY      + D ++   SDD  KL+      + +G+Y CV +N               K+  + V       +    K +   +GE+  + C       G P P + W+            +   + +D  T    +   +  G Y C+A+++ G     +  L  +  A  I+ +  N +VFL      + IS +C+ + +   +  + +   G+E   P +     + HR                 LY       T  +   NG+Y C   N  G A
Sbjct: 1227 TIREDGALVLQNVKLENTGVFVCQVSNLAGEDSL--SYTLTVHEKPKIIS-EVPGVVDVVKGFT-IEIPCRATG--VPEVIRTW--NKNGID-LKMDEKKF---SVDNLGTLRIYEADK-NDIGNYNCVVTNEAGT-SQMTTHVDVQEPPIILPSTQTNNTAVVGDRVELKCYVEASPPASVTW-FRRGIAIGTDTKGYVV----ESDGTLVIQSASVEDATIYTCKASNPAGKAEANLQVTVIASPDIKDPDVVTQESIKESHPFSLYCPVFSNPLPQ-ISWYLNDKPLIDDKTSWKTSDDKRKLHVFKAKITDSGVYKCVARNAA---------GEGSKSFQVEVIVPLNLDESKYKKKVFAKEGEEVTLGCPVS----GFPVPQINWVVDGTVVEPGKKYKGATLSNDGLTLHFDSVSVKQEGNYHCVAQSK-GNILDIDVELSVL--AVPIVGEDDNLEVFLG-----KDISLSCDLQTESDDKTTFVWSINGSESDRPDNVQIPSDGHR-----------------LYI------TDAKPENNGKYMCRVTNSAGKA 1724          

HSP 7 Score: 78.9518 bits (193), Expect = 2.814e-14
Identity = 130/565 (23.01%), Postives = 214/565 (37.88%), Query Frame = 3
Query:  330 ESQITISHESRLTIHNISSDNSGYYVCRVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQ--EQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSL-LNIKYKPKI-KPFVPNTVNAMINQTIDLSCEVYANPAATIQWVIMQGLQI--IDSSKFIAK---------IRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLIHIDSNYRVPKNSNYSINCRVTSNPPPEF------IKWYKLSNMTDSSA---VLISDDA-KLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWI-------DVETKDIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIA 1928
            +S+ TI +   L I++++++++G Y C + +          A   +  P         K  N+++  E + I + C V+   P P IS +   D  +      S +   S I    A        L + G  +C A N+AG  T +  L L I   PKI K  +     A++ +TI L C +   P   + W    G+ I   DS   +A+         ++    G   C A N  G       L+VL  P   I       K    +I          +       + W K S   D      V ISDD  KL        + G+Y C+  N     + + K         + ++    +PQ+  N        +  I++C+      G PFP++KW+       D E   IV   +  Q++ ++S H GK+ C+AEN+ G    +E  +V     P +++   +  +      L ++I+  C   G P  + +W  G   I      F   D       RIS    +L I            L+ +  G Y C+A N  G +
Sbjct: 3434 DSRHTIVNNYDLKINSVTTEDAGQYSC-IAVNEAGNLTTHYAAEVIGKP-----TFVRKGGNLYEVIENDTITMDCGVTSR-PLPSIS-WFRGDKPVYLYDRYSISPDGSHITINKA-------KLSDGGKYICRASNEAG--TSDIDLILKILVPPKIDKSNIIGNPLAIVARTIYLECPISGIPQPDVIWT-KNGMDINMTDSRVILAQNNETFGIENVQVTDQGRYTCTATNRGGKASHDFSLDVLSPPEFDIHGTQPTIKREGDTITLTCPIKLAEDIADQVMDVSWTKDSRALDGDLTDNVDISDDGRKLTISQASLENAGLYTCIALNRAGEASLEFK-VEILSPPVIDISRNDVQPQVAVN--------QPTIMRCAVT----GHPFPSIKWLKNGKEVTDDENIRIVEQGQVLQILRTDSDHAGKWSCVAENDAG---VKELEMVLDVFTPPVVSVKSDNPI----KALGETITLFCNASGNPYPQLKWAKGGSLI------FDSPD-----GARISLKGARLDI----------PHLKKTDVGDYTCQALNAAGTS 3939          

HSP 8 Score: 77.0258 bits (188), Expect = 9.867e-14
Identity = 148/650 (22.77%), Postives = 248/650 (38.15%), Query Frame = 3
Query:  516 VFQEQEFIAIQCSVSGG---IPPPKISMYIVKDNAI-----KRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQT--IDLSCEVYANPAATIQWVIMQGLQIIDSSKFIAKIRPDMD--------GPLICEAKNYLGSTKETVQLNVLRDPLIH---IDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMTDSSAVLISDDAKLNFILIDRSST---GMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWI-------DVETKDIVSDMETFQVINSESRHNGKYKCLAENEIGKS-FSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTC------EFRGQPLYEARWRYGNEKIPPSSQDFKIEDHR--NISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPG-LKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQPDPPYDLRVTDFE 2342
            +F+E E I + C VS G   I   K  + + +++AI      RL I+++N               R H         C A N AG ++K+  ++ ++  PKIK  V   V   IN+   I L+C+   NP  T +W   QG  +   + F+      +         G   C A N +G   +T+ ++V   P       +S   V    + ++ C V  +     I W        +    +   A   F+ I  + T   G YAC   N         +   + KT  L V   P      G    ++++    ++ C       G+P P + W        D+++  ++S+ + F+++++E  H G Y C+A+N++G +  S +  ++T    P I    KN    +    LP    F C       F+GQ +    W    + I     D + ED R   +SN+R      +LTI           ++  +  G+Y+C   N  G  S D F      P     +   K   ++  + V + C A       I W+K  E I +                 E   D+   G L  N+L I  ++  D   Y+C A N  G  + +  V   + P    D   +D+E
Sbjct: 1837 IFRESETITLDCPVSLGDFQITWMKQGLPLTENDAIFTLDNTRLTILNAN---------------RDH----EDIYTCVANNTAGQVSKDFDVV-VQVLPKIKNAV---VTLEINEGEEIILTCDAEGNPTPTAKWDFNQG-DLPKEAVFVNNNHTVVVNNVTKYHTGVYKCYATNKVGQAVKTINVHVRTKPRFESGLTESELTVNLTRSITLECDV-DDAIGVGISWTVNGKPFLAETDGVQTLAGGRFLHIVSAKTDDHGSYACTVTN---------EAGVATKTFNLFVQVPPTIVNEGGE--YTVIENNSLVLPCEVT----GKPNPVVTWTKDGRPVGDLKSVQVLSEGQQFKIVHAEIAHKGSYICMAKNDVGTAEISFDVDIIT---RPMIQKGIKNIVTAIKGGALP----FKCPIDDDKNFKGQII----WLRNYQPI-----DLEAEDARITRLSNDR------RLTI----------LNVTENDEGQYSCRVKNDAGENSFD-FKATVLVPPTIIMLDKDKNKTAVEHSTVTLSCPATGKPEPDITWFKDGEAIHI-----------------ENIADIIPNGELNGNQLKITRIKEGDAGKYTCEADNSAGSVEQDVNVNVITIPKIEKDGIPSDYE 2396          

HSP 9 Score: 72.0182 bits (175), Expect = 3.503e-12
Identity = 137/614 (22.31%), Postives = 240/614 (39.09%), Query Frame = 3
Query:  318 VPDSESQITISHESRLTIHNISSDNSGYYVCRVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLE-NRGFVMCTAINDAGVLTKNTSLLNIKYKPKI-KPFVPNTVNAMINQTIDLSCEVYANPAATIQWVI--------MQGLQIIDSSKFIA--KIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLIHIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMTDSS--AVLISDDAKLNFILIDRS-STGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGN--KIISIMKGEKFIIKCSTDRKSPGEPFPTLKWI-------DVETKDIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNE 2087
            V D E+   +     L I    SD++G + C   +      +  L +V     P  +S   +  I    E   I + C+ SG  P P++     K  ++    I  S  P+     +    L  PH  + + G   C A+N AG    + S+ ++   P+I +  +  +      Q++ L C     P   ++W +          G+ +   S FI    +     G   C A+N  GS      ++V++ P+I      +V +     I C V   P P+ + W +  N  ++    V    D ++  I+  RS  +G+Y C       S T +   A    T+ + V+     P+I+ +   +++   G KF + C+      G P P + W        D E    +    T  +  +E RH   Y+C A+N+ G   + E  + T+  AP I T   NR  +++ S+  +++   CE   +    +  + G   +P ++  F  ED++ I                      +  S R S  G Y+C A N+ G A+    L +   P I    R + +      + V + C+A  +    I WYK NE
Sbjct: 3808 VTDDENIRIVEQGQVLQILRTDSDHAGKWSC---VAENDAGVKELEMVLDVFTPPVVSVKSDNPIKALGET--ITLFCNASGN-PYPQLKW--AKGGSL----IFDS--PDGARISLKGARLDIPHLKKTDVGDYTCQALNAAGTSEASVSV-DVLVPPEINRDGIDMSPRLPAQQSLTLQCLAQGKPVPQMRWTLNGTALTHSTPGITVASDSTFIQINNVSLSDKGVYTCYAENVAGSDNLMYNVDVVQAPVISNGGTKQVIEGELAVIECLVEGYPAPQ-VSWLRNGNRVETGVQGVRYVTDGRMLTIIEARSLDSGIYLC-------SATNEAGSAQQAYTLEVLVS-----PKIITSTPGVLTPSSGSKFSLPCAVR----GYPDPIISWTLNGNDIKDGENGHTIGADGTLHIEKAEERHL-IYECTAKNDAGAD-TLEFPVQTI-VAPKISTS-GNR--YINGSEGTETV-IKCEIESESSEFSWSKNGVPLLPSNNLIFS-EDYKLI----------------------KILSTRLSDQGEYSCTAANKAGNATQKTNLNVGVAPKIMERPRTQVVHKG---DQVTLWCEASGVPQPAITWYKDNE 4356          

HSP 10 Score: 70.4774 bits (171), Expect = 1.012e-11
Identity = 144/689 (20.90%), Postives = 249/689 (36.14%), Query Frame = 3
Query:  330 ESQITISHESRLTIHNISSDNSGYYVCRVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIK-PFVPNTVNAMINQTIDLSCEVYANPAATIQWVIMQGLQIIDSSKF----------IAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLIHIDSNYR----VPKNSNYSINCRVTSNPPPEFIKWYKLSNMTD-----SSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKW-IDVETKD------IVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQ--HKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQI-FTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQP 2306
            E + ++ +   L I+    ++ G Y C VT    +  + +   V V+ PP  I     ++ N     + + ++C V    PP  ++ +        R  I         + E     + +   +E+     C A N AG    N  +  I   P IK P V    +   +    L C V++NP   I W +     I D + +          + K +    G   C A+N  G   ++ Q+ V+  PL   +S Y+      +    ++ C V+  P P+ I W     + +       A L +D   L+F  +     G Y CV Q     + G     + E ++          P +  +  + +  G+   + C  D ++  +   T  W I+    D      I SD     + +++  +NGKY C   N  GK+    T  + V E P  +      N+K+  +       I   C+  G P     W+     +          D   + +E +S +   KLT                  + + +C A+N+ G AS D+F+     P  K       I        + + C   S+    I W K      LP  ++  +IF  + T+                LTI +   D    Y+CVA+N  G    +F+VV +  P
Sbjct: 1315 EKKFSVDNLGTLRIYEADKNDIGNYNCVVTNEAGTSQMTTH--VDVQEPP--IILPSTQTNNTAVVGDRVELKCYVEAS-PPASVTWF--------RRGIAIGTDTKGYVVESDGTLVIQSASVEDATIYTCKASNPAGKAEANLQVTVIA-SPDIKDPDVVTQESIKESHPFSLYCPVFSNPLPQISWYLNDKPLIDDKTSWKTSDDKRKLHVFKAKITDSGVYKCVARNAAGEGSKSFQVEVIV-PLNLDESKYKKKVFAKEGEEVTLGCPVSGFPVPQ-INWVVDGTVVEPGKKYKGATLSNDGLTLHFDSVSVKQEGNYHCVAQ-----SKGNILDIDVELSVLAV-------PIVGEDDNLEVFLGKDISLSC--DLQTESDDKTTFVWSINGSESDRPDNVQIPSDGHRLYITDAKPENNGKYMCRVTNSAGKA--ERTLTLDVLEPPVFVEPVFEANQKLIGN-----NPIILQCQVTGNPKPTVIWKIDGNDV----------DKSWLFDESLSLLRIEKLT----------------GKSAQISCTAENKAGTASRDFFIQNIAAPTFKNEGDQETIFRE--SETITLDCPV-SLGDFQITWMKQG----LPL-TENDAIFTLDNTR----------------LTILNANRDHEDIYTCVANNTAGQVSKDFDVVVQVLP 1916          

HSP 11 Score: 67.781 bits (164), Expect = 6.611e-11
Identity = 115/559 (20.57%), Postives = 209/559 (37.39%), Query Frame = 3
Query:  702 ENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQWVI----MQGLQIIDSSKFIAKIRP--DMDGPLICEAKNYLGSTKETVQLNVLRDPLIHIDSNYRV--PKNSNYSINCRVTSNPPPEF-IKWYKLS-NMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIIS--IMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETKDIVSDM------ETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTN------EHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQP 2306
            EN G  MC   N AG   +  +L  ++    ++P        + N  I L C+V  NP  T+ W I    +    + D S  + +I         + C A+N  G+      +  +  P    + +      ++   +++C V+     +F I W K    +T++ A+   D+ +L  +  +R    +Y CV  N    T G+       K   + V   PK    + N +++  I +GE+ I+ C  +    G P PT KW D    D+  +        T  V N    H G YKC A N++G++   +T  V V   P   +     ++ +   +L +SI+  C+          W         + + F  E              T     L    F    S ++  +G Y C   N  G+A+  + L ++ PP I        +   +  N +++ C+        + W K        + + +  + +++ I  AE+                              +Y C+A ND G  +++F+V   ++P
Sbjct: 1708 ENNGKYMCRVTNSAGKAERTLTLDVLEPPVFVEPVFEANQKLIGNNPIILQCQVTGNPKPTVIWKIDGNDVDKSWLFDESLSLLRIEKLTGKSAQISCTAENKAGTASRDFFIQNIAAPTFKNEGDQETIFRESETITLDCPVSLG---DFQITWMKQGLPLTENDAIFTLDNTRLTILNANRDHEDIYTCVANN----TAGQ-----VSKDFDVVVQVLPK----IKNAVVTLEINEGEEIILTCDAE----GNPTPTAKW-DFNQGDLPKEAVFVNNNHTVVVNNVTKYHTGVYKCYATNKVGQAV--KTINVHVRTKPRFESGLTESELTV---NLTRSITLECDVDDAIGVGISWTV-------NGKPFLAE--------------TDGVQTLAGGRFLHIVSAKTDDHGSYACTVTNEAGVATKTFNLFVQVPPTIVNEGGEYTV---IENNSLVLPCEVTGKPNPVVTWTKDGRPVGDLKSVQVLSEGQQFKIVHAEIAHK--------------------------GSYICMAKNDVGTAEISFDVDIITRP 2190          

HSP 12 Score: 63.929 bits (154), Expect = 9.289e-10
Identity = 127/631 (20.13%), Postives = 239/631 (37.88%), Query Frame = 3
Query:  525 EQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIK-----YKPKIKPFVPNTVNAM-------INQTID--------LSCEVYANPAATIQWVIMQGLQII----------DSSKFIAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLIH-IDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETKDI---VSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNR-KVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHR-NISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQPD 2309
            E + + + C V  G P P I ++I  D  ++         P   I    ++   R    ++ G   C A++ AG  T + ++  IK     YKP+       T++ M       +N T D        + C V   P   I W  + G  I           D++  + K      G   C+A N  G  ++   + ++  P+I    S++ +  +  ++I C V  +P P  I W  L +    +  ++++D  L    ++ +  G + C  QN            N  +T+ LTV   P        KI   ++ +  +++C      P     T +   ++++ I   + +     + N +  + G + C   N  G+     T  +TV+E P II++      V   ++     I   C   G P     W         +  D K+++ + ++ N    +I+     D+                G YNC   N  G + +   + ++ PPII  + +    A   ++  V ++C  E+     + W++    I +   +K Y + E++ T                 L I+    +D + Y+C ASN  G  + N +V   + PD
Sbjct:  900 EGQDLTLSCVVVLGTPKPSI-VWIKDDKPVEE-------GPTIKIEGGGSLLRLRGGNPKDEGKYTCIAVSPAGNSTLHINVQLIKKPEFVYKPEGGIVFKPTISGMDEKHVAVVNSTHDVLDGEGFAIPCVVSGTPPPIITWY-LDGRPITPNSRDFTVTADNTLIVRKADKSYSGVYTCQATNSAGDNEQKTTIRIMNTPMISPGQSSFNMVVDDLFTIPCDVYGDPKP-VITW--LLDDKPFTEGVVNEDGSLTIPNVNEAHRGTFTCHAQNAA---------GNDTRTVTLTVHTTPTINAENQEKIA--LQNDDIVLECPAKALPPPVRLWTYEGEKIDSQLIPHTIREDGALVLQNVKLENTGVFVCQVSNLAGEDSLSYT--LTVHEKPKIISEVPGVVDVVKGFT-----IEIPCRATGVPEVIRTWNK-------NGIDLKMDEKKFSVDNLGTLRIYEADKNDI----------------GNYNCVVTNEAGTSQMTTHVDVQEPPIILPSTQTNNTAVVGDR--VELKCYVEASPPASVTWFRRG--IAIGTDTKGY-VVESDGT-----------------LVIQSASVEDATIYTCKASNPAGKAEANLQVTVIASPD 1455          

HSP 13 Score: 61.6178 bits (148), Expect = 4.519e-9
Identity = 132/640 (20.62%), Postives = 233/640 (36.41%), Query Frame = 3
Query:  153 ITPNNATVNSKENLTLECVVDQQ-NIVYSWEY--KPFHIKSNIMSKVLGFCYFEKPCVVPDSESQITISHESRLTIHNISSDNSGYYVCRVTLLTTSEHIISLAV--VTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSL-LNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQWVIMQGLQIIDSSKFIAKI--------------RPDMDGPLICEAKNYLGSTKETVQLNVLRDPLIHI---DSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMTDSSAVLISDDA-----------KLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSP--------GEPFPTLKWIDVETKDIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFL 1946
            +T +  TVN   ++TLEC VD    +  SW    KPF  +++ +  + G  +                     L I +  +D+ G Y C V    T+E  ++     + V+ PP  ++     ++    E   + + C V+G   P  +  +      +  LK +        ++E     +     + ++G  +C A ND G  T   S  ++I  +P I+  + N V A+    +   C +  +     Q + ++  Q ID     A+I                + +G   C  KN  G      +  VL  P I +   D N    ++S  +++C  T  P P+ I W+K     D  A+ I + A           +L    I     G Y C   N            + E+ + + V   PK  +          + E+ +I C    + P        G+P  + K+  V+T      +  F++  ++S    KY C+A NE G    +    V++  APS    +  R++ ++  + P ++   C  +G P     W      I P+ +    +  R +                      Q +    S  GRY C A N  G   L+  L
Sbjct: 2011 LTESELTVNLTRSITLECDVDDAIGVGISWTVNGKPFLAETDGVQTLAGGRF---------------------LHIVSAKTDDHGSYACTV----TNEAGVATKTFNLFVQVPPTIVNEGGEYTV---IENNSLVLPCEVTG--KPNPVVTWTKDGRPVGDLKSV------QVLSEGQQFKIVHAE-IAHKGSYICMAKNDVG--TAEISFDVDIITRPMIQKGIKNIVTAIKGGALPFKCPIDDDKNFKGQIIWLRNYQPIDLEAEDARITRLSNDRRLTILNVTENDEGQYSCRVKNDAGENSFDFKATVLVPPTIIMLDKDKNKTAVEHSTVTLSCPATGKPEPD-ITWFK-----DGEAIHIENIADIIPNGELNGNQLKITRIKEGDAGKYTCEADN---------SAGSVEQDVNVNVITIPKIEKDGIPSDYESQQNERVVISCPVYARPPAKITWLKAGKPLQSDKF--VKTSANGQKLYLFKLRETDSS---KYTCIATNEAGT--DKRDFKVSMLVAPSFDEPNIVRRITVNSGN-PSTLH--CPAKGSPSPTITWLKDGNAIEPNDRYVFFDAGRQL----------------------QISKTEGSDQGRYTCIATNSVGSDDLENTL 2564          

HSP 14 Score: 55.4546 bits (132), Expect = 3.501e-7
Identity = 65/315 (20.63%), Postives = 121/315 (38.41%), Query Frame = 3
Query:  357 SRLTIHNISSDNSGYYVCRVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINV-FQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYL--ENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQWVIMQ-------GLQIIDSSKF--IAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLIHIDSN-YRVPKNSNYSINCRVTSNPPPEFIKWYK-LSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNI 1259
            S  TI  +S  ++G Y CRV +     + +   V T ++PP+       ++++V   E  F+      +G +              I+  +  ++ F              + H++   + G   C A N  G+ T+   L +I   P +K   P  V   + + ++LSCE   +P   + W             Q+   SKF  I       +G   C A +  G  ++T  L +   P + I  N   V +    S  C+     P   I+W+K   ++      +  ++ +L+ +       G Y+CV +N+
Sbjct:  482 SVWTIPELSLKDAGEYECRV-ISNNGNYSVKTRVETRESPPEIFGV---RNVSVPLGEAAFLHCSTRSAGEV-------------EIRWTRYGATVFNGPNTERNPTNGTLKIHHVTRADAGVYECMARNAGGMSTRKMRL-DIMEPPSVK-VTPQDVYFNMREGVNLSCEAMGDPKPEVHWYFKGRHLLNDYKYQVGQDSKFLYIRDATHHDEGTYECRAMSQAGQARDTTDLMLATPPKVEIIQNKMMVGRGDRVSFECKTIRGKPHPKIRWFKNGKDLIKPDDYIKINEGQLHIMGAKDEDAGAYSCVGENM 777          

HSP 15 Score: 54.6842 bits (130), Expect = 5.999e-7
Identity = 96/423 (22.70%), Postives = 164/423 (38.77%), Query Frame = 3
Query:  516 VFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMIN--QTIDLSCEVYANPAATIQWVIMQGL--------QIIDSSKFIAKIRPDMDGPLICEAKNYLGSTKETVQLNV----LRDPLIHIDSNYRV-PKNSNYSINCRVTSNPPPEFIKWYKLSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQ-ILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWID----VETKDIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNE----APSIITQHKNRKVFLSYS-DLPQSISFTCEFRGQPL 1709
            V  + + + + C  SG +P P I+ Y  KDN     +++++   + T        ++        G   C A N      ++  L+ +   P++   VP  +N   N  QT+ LSC     P   I W+    +        QI+ ++  I  + PD DG   C AK+  G    T +L V     R   I ++ + +  PK + Y I+   T +  P+ + W  +            +D+ LN  +        Y C+         G R    S +T+ L        PQ I+  K  +   G    ++CS    + G P PT+ W      +E           +V  + +  +G+Y C+A+N +G S    +A + V+      P   +  KN  V   Y  +   S   T E+ G P+
Sbjct: 4328 VVHKGDQVTLWCEASG-VPQPAITWY--KDN-----ELLTNTGVDETATTKKKSVIFSSISPSQAGVYTCKAENWVASTEEDIDLI-VMIPPEV---VPERMNVSTNPRQTVFLSCNATGIPEPVISWMRDSNIAIQNNEKYQILGTTLAIRNVLPDDDGFYHCIAKSDAGQKIATRKLIVNKPSDRPAPIWVECDEKGKPKKTEYMIDRGDTPDDNPQLLPWKDV------------EDSSLNGSI-------AYRCM--------PGPR----SSRTVLLHAA-----PQFIVKPKNTTAAIGAIVELRCS----AAGPPHPTITWAKDGKLIEDSKFEIAYSHLKVTLNSTSDSGEYTCMAQNSVGSSTV--SAFINVDNNILPTPKPSSNQKNVAVITCYERNQAYSRGLTWEYNGVPM 4696          
BLAST of hemicentin-1 isoform X2 vs. Ensembl Celegans
Match: him-4 (High Incidence of Males (Increased X chromosome loss) [Source:UniProtKB/TrEMBL;Acc:A4UVK0])

HSP 1 Score: 104.375 bits (259), Expect = 5.491e-22
Identity = 157/706 (22.24%), Postives = 278/706 (39.38%), Query Frame = 3
Query:  306 KPCVVPDSESQIT-ISHESRLTIHNISSDNSGYYVCRVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLE-NRGFVMCTAINDAGVLTKNTSLLNIKYKPKI-KPFVPNTVNAMINQTIDLSCEVYANPAATIQWVIMQGLQIIDSSKFIA-----------KIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLI---HIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMTDSSAVLISDDA--KLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVE-TKD----------------IVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQ--HKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAE-SISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQP 2306
            +P  +   +++IT +S++ RLTI N++ ++ G Y CRV            A V V  PP  I    +K+     E   + + C  +G  P P I+ +  KD     ++ I+   PN    E++   L      E + G   C A N AG + ++ ++ N+   PKI K  +P+   +  N+ + +SC VYA P A I W  ++  + + S KF+            K+R        C A N  G+ K   ++++L  P     +I     V   +  +++C    +P P  I W K  N  + +   +  DA  +L     + S  G Y C+  N    + G     N+ + +          P I G +  ++   E F  +   D  S G        +DVE  KD                I S  +    +++    +G+Y C+  N  G+  +R+     VN+ PSI  +    N +  + Y      +   C   G P  +  W + ++ + P S  +++ ++        SQ+                        G Y CEA N  G A  D+ + +  PP  +   + R+   +   + +++ C AE S+    + W+  +E +     + KY+  E                     L + +++ DD   Y C A N+ GI K  F+++    P
Sbjct: 2231 QPIDLEAEDARITRLSNDRRLTILNVTENDEGQYSCRVKNDAGENSFDFKATVLV--PPTIIMLDKDKNKTAV-EHSTVTLSCPATGK-PEPDITWF--KDGEAIHIENIADIIPN---GELNGNQLKITRIKEGDAGKYTCEADNSAGSVEQDVNV-NVITIPKIEKDGIPSDYESQQNERVVISCPVYARPPAKITW--LKAGKPLQSDKFVKTSANGQKLYLFKLRETDSSKYTCIATNEAGTDKRDFKVSMLVAPSFDEPNIVRRITVNSGNPSTLHCPAKGSPSPT-ITWLKDGNAIEPNDRYVFFDAGRQLQISKTEGSDQGRYTCIATN----SVGSDDLENTLEVII--------PPVIDGERREAVAVIEGFSSELFCDSNSTG--------VDVEWQKDGLTINQDTLRGDSFIQIPSSGKKMSFLSARKSDSGRYTCIVRNPAGE--ARKLFDFAVNDPPSISDELSSANIQTIVPY----YPVEINCVVSGSPHPKVYWLFDDKPLEPDSAAYELTNNGETLKIVRSQV---------------------EHAGTYTCEAQNNVGKARKDFLVRVTAPPHFE---KEREEVVARVGDTMLLTCNAESSVPLSSVYWHAHDESVQNGVITSKYAANE-------------------KTLNVTNIQLDDEGFYYCTAVNEAGITKKFFKLIVIETP 2854          

HSP 2 Score: 94.3597 bits (233), Expect = 5.374e-19
Identity = 148/706 (20.96%), Postives = 273/706 (38.67%), Query Frame = 3
Query:  306 KPCVVPDSESQITISHESRLTIHNISSDNSGYYVCRVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLW-RPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEV-YANPAATIQWV-----IMQGLQI-IDSSKFIAKIR---PDMDGPLICEAKNYLGSTKETVQLNVLRDP-----------------------LIHIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSN--MTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVE---TKDIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQP 2306
            K  + PD   +I   +E +L I     +++G Y C    +   +  + +A ++V   P  I       +N+ ++   + +QC ++ GIPPP+I          ++  ++ +   N    +++  +L      +E++G   C A N  G  +++T+L+       +   VP     +  Q + LSC V    P  +I W+     + +G  I I+    + ++R   P  +G   C A +  G++   + + +++ P                       +  ++S + V     ++I C V+  PPP  I WY        +S    ++ D  L     D+S +G+Y C   N    + G     N +KT    +      P   G    +++  + F I C       G+P P + W+  +   T+ +V++  +  + N    H G + C A+N  G      T  +TV+  P+I  +++  K+ L   D    I   C  +  P     W Y  EKI     D ++  H               TI        Q   L ++  G + C+  N  G  SL Y L +   P I   +    +   +    + I C+A  +  +   W K    I L    KK+S+                    +  L I + + +D  NY+CV +N+ G  ++   V  +  P
Sbjct:  741 KDLIKPDDYIKI---NEGQLHIMGAKDEDAGAYSCVGENMAGKD--VQVANLSVGRVPTIIESPHTVRVNIERQ---VTLQC-LAVGIPPPEIEW--------QKGNVLLATLNNPRYTQLADGNLLITDAQIEDQGQFTCIARNTYGQQSQSTTLMVTGLVSPVLGHVPPEEQLIEGQDLTLSCVVVLGTPKPSIVWIKDDKPVEEGPTIKIEGGGSLLRLRGGNPKDEGKYTCIAVSPAGNSTLHINVQLIKKPEFVYKPEGGIVFKPTISGMDEKHVAVVNSTHDVLDGEGFAIPCVVSGTPPP-IITWYLDGRPITPNSRDFTVTADNTLIVRKADKSYSGVYTCQATN----SAGD----NEQKTTIRIMNTPMISP---GQSSFNMVVDDLFTIPCDVY----GDPKPVITWLLDDKPFTEGVVNEDGSLTIPNVNEAHRGTFTCHAQNAAGND--TRTVTLTVHTTPTINAENQ-EKIALQNDD----IVLECPAKALPPPVRLWTYEGEKI-----DSQLIPH---------------TIREDGALVLQNVKLENT--GVFVCQVSNLAGEDSLSYTLTVHEKPKIISEVP--GVVDVVKGFTIEIPCRATGVPEVIRTWNKNG--IDLKMDEKKFSVD------------------NLGTLRIYEADKNDIGNYNCVVTNEAGTSQMTTHVDVQEPP 1362          

HSP 3 Score: 86.6557 bits (213), Expect = 1.221e-16
Identity = 133/549 (24.23%), Postives = 219/549 (39.89%), Query Frame = 3
Query:  249 PFHIKSNIMSKVLGFCYFEKP-CVVPDSESQITISHESR-LTIHNISSDNSGYYVC-------------RVTLLTTS-------EH---IISLAVVTVKTPPKSISFHFNK--------SINVFQEQEFI----AIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQWVIMQGLQIIDSSKFIAKI-----RPDMD-------GPLICEAKNYLGSTKETVQLNVLRDPLIH---IDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMTDSSAVLISDDAKLNFILIDR---SSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWI--------DVETKDIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQP 1706
            P  +  +I  +V+   + +    +  D    + IS + R LTI   S +N+G Y C             +V +L TS       EH    I+  +  V T P+ +S   N         S N  Q Q  +     ++C+V+G  P P I         +K  K ++ +     + +   + + R    ++ G   C A NDAGV  +   +L++   P +     N + A+  +TI L C    NP   ++W   +G  +I  S   A+I     R D+        G   C+A N  G+++ +V ++VL  P I+   ID + R+P   + ++ C     P P+ ++W         S   I+  +   FI I+    S  G+Y C  +N+          A S+  M+       + P I       +++GE  +I+C  +    G P P + W+         V+    V+D     +I + S  +G Y C A NE G +    T  V V  +P IIT         S S      S  C  RG P
Sbjct: 3690 PIKLAEDIADQVMDVSWTKDSRALDGDLTDNVDISDDGRKLTISQASLENAGLYTCIALNRAGEASLEFKVEILCTSLATVKCREHENNYIARHMPHVVTKPREMSQSANAYPPPVIDISRNDVQPQVAVNQPTIMRCAVTGH-PFPSIKW-------LKNGKEVTDDENIRIVEQGQVLQILRTDS-DHAGKWSCVAENDAGV-KELEMVLDVFTPPVVSVKSDNPIKAL-GETITLFCNASGNPYPQLKWA--KGGSLIFDSPDGARISLKGARLDIPHLKKTDVGDYTCQALNAAGTSEASVSVDVLVPPEINRDGIDMSPRLPAQQSLTLQCLAQGKPVPQ-MRWTLNGTALTHSTPGITVASDSTFIQINNVSLSDKGVYTCYAENV----------AGSDNLMYNVDVV--QAPVISNGGTKQVIEGELAVIECLVE----GYPAPQVSWLRNGNRVETGVQGVRYVTDGRMLTIIEARSLDSGIYLCSATNEAGSAQQAYTLEVLV--SPKIITSTPGVLTPSSGSKF----SLPCAVRGYP 4202          

HSP 4 Score: 83.9593 bits (206), Expect = 8.330e-16
Identity = 145/704 (20.60%), Postives = 267/704 (37.93%), Query Frame = 3
Query:  291 FCYFEKPCVVPDSESQITISHESRLTIHNISSDNSGYYVCRVTLLTTSEHIISLA----VVTVKTPPKSISFHFNKSIN--VFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKY-----KPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQWVI-------MQGLQIIDSSKFIAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLIHID-SNYRVPKNSNYSINCRV--TSNPPPEFIKWYKLSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKI--ISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETKDIV---------SDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQP 2306
            +  F+   + PDS +    ++   L I     +++G Y C       +++ +  A    +V V  PP     HF K     V +  + + + C+    +P   +  +   D +++   I S    N     V+ I L      ++ GF  CTA+N+AG+  K   L+ I+      + K+ P +       + + + L C     P  TI ++           + II S+  I   + +++G   C A+N  G +++ + + VL  P +  +  N  V      ++ C +  TS     + + +      D  A   SD +KL  +         Y+C+  N           A   + +F      P  P+I G+      I+      I C T+    G P P + W  ++ K I+          D+ + ++ N +    G+Y C+AEN+ G++   +   V ++E P ++   +  +V        +  +  CE  G P     W    E   P + D             + Q  TKL+  L+ R    +T+L  +  G Y C A N+ G +     + +  PP I+      ++      N  M+ CQ       ++ W +  + I                       + F P L      I++    D   YSC+ASN+ G    +F +   ++P
Sbjct: 2701 YWLFDDKPLEPDSAAYELTNNGETLKIVRSQVEHAGTYTCE------AQNNVGKARKDFLVRVTAPP-----HFEKEREEVVARVGDTMLLTCNAESSVPLSSVYWH-AHDESVQNGVITSKYAANEKTLNVTNIQL------DDEGFYYCTAVNEAGITKKFFKLIVIETPYFLDQQKLYPII-------LGKRLTLDCSATGTPPPTILFMKDGKRLNESDEVDIIGSTLVIDNPQKEVEGRYTCIAENKAGRSEKDMMVEVLLPPKLSKEWINVEVQAGDPLTLECPIEDTSGVHITWSRQFGKDGQLDMRAQSSSDKSKLYIMQATPEDADSYSCIAVN----------DAGGAEAVFQVTVNTP--PKIFGDSFSTTEIVADTTLEIPCRTE----GIPPPEISWF-LDGKPILEMPGVTYKQGDL-SLRIDNIKPNQEGRYTCVAENKAGRA--EQDTYVEISEPPRVVMASEVMRVVEG-----RQTTIRCEVFGNPEPVVNWLKDGE---PYTSD-------------LLQFSTKLSY-LHLR----ETTL--ADGGTYTCIATNKAGESQTTTDVEVLVPPRIED---EERVLQGKEGNTYMVHCQVTGRPVPYVTWKRNGKEI-----------------------EQFNPVLH-----IRNATRADEGKYSCIASNEAGTAVADFLIDVFTKP 3300          

HSP 5 Score: 80.1073 bits (196), Expect = 1.172e-14
Identity = 122/562 (21.71%), Postives = 225/562 (40.04%), Query Frame = 3
Query:  342 TISHESRLTIHNISSDNSGYYVCRVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQWVIMQGLQI-IDSSKFIAKIRPDMDGPLI-------------CEAKNYLGSTKETVQLNVLRDPLIH---IDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSN--MTDSSAVLISDDA-KLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWI----------DVETKDIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRY---GNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIA 1928
            TI  +  L + N+  +N+G +VC+V+ L   + +     +TV   PK IS      ++V +    I I C  +G   P  I  +    N I  LK+    F   ++  +  + ++      + G   C   N+AG  ++ T+ ++++  P I P       A++   ++L C V A+P A++ W   +G+ I  D+  ++     + DG L+             C+A N  G  +  +Q+ V+  P I    + +   + ++  +S+ C V SNP P+ I WY      + D ++   SDD  KL+      + +G+Y CV +N               K+  + V       +    K +   +GE+  + C       G P P + W+            +   + +D  T    +   +  G Y C+A+++ G     +  L  +  A  I+ +  N +VFL      + IS +C+ + +   +  + +   G+E   P +     + HR                 LY       T  +   NG+Y C   N  G A
Sbjct: 1227 TIREDGALVLQNVKLENTGVFVCQVSNLAGEDSL--SYTLTVHEKPKIIS-EVPGVVDVVKGFT-IEIPCRATG--VPEVIRTW--NKNGID-LKMDEKKF---SVDNLGTLRIYEADK-NDIGNYNCVVTNEAGT-SQMTTHVDVQEPPIILPSTQTNNTAVVGDRVELKCYVEASPPASVTW-FRRGIAIGTDTKGYVV----ESDGTLVIQSASVEDATIYTCKASNPAGKAEANLQVTVIASPDIKDPDVVTQESIKESHPFSLYCPVFSNPLPQ-ISWYLNDKPLIDDKTSWKTSDDKRKLHVFKAKITDSGVYKCVARNAA---------GEGSKSFQVEVIVPLNLDESKYKKKVFAKEGEEVTLGCPVS----GFPVPQINWVVDGTVVEPGKKYKGATLSNDGLTLHFDSVSVKQEGNYHCVAQSK-GNILDIDVELSVL--AVPIVGEDDNLEVFLG-----KDISLSCDLQTESDDKTTFVWSINGSESDRPDNVQIPSDGHR-----------------LYI------TDAKPENNGKYMCRVTNSAGKA 1724          

HSP 6 Score: 77.0258 bits (188), Expect = 1.139e-13
Identity = 148/650 (22.77%), Postives = 248/650 (38.15%), Query Frame = 3
Query:  516 VFQEQEFIAIQCSVSGG---IPPPKISMYIVKDNAI-----KRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQT--IDLSCEVYANPAATIQWVIMQGLQIIDSSKFIAKIRPDMD--------GPLICEAKNYLGSTKETVQLNVLRDPLIH---IDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMTDSSAVLISDDAKLNFILIDRSST---GMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWI-------DVETKDIVSDMETFQVINSESRHNGKYKCLAENEIGKS-FSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTC------EFRGQPLYEARWRYGNEKIPPSSQDFKIEDHR--NISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPG-LKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQPDPPYDLRVTDFE 2342
            +F+E E I + C VS G   I   K  + + +++AI      RL I+++N               R H         C A N AG ++K+  ++ ++  PKIK  V   V   IN+   I L+C+   NP  T +W   QG  +   + F+      +         G   C A N +G   +T+ ++V   P       +S   V    + ++ C V  +     I W        +    +   A   F+ I  + T   G YAC   N         +   + KT  L V   P      G    ++++    ++ C       G+P P + W        D+++  ++S+ + F+++++E  H G Y C+A+N++G +  S +  ++T    P I    KN    +    LP    F C       F+GQ +    W    + I     D + ED R   +SN+R      +LTI           ++  +  G+Y+C   N  G  S D F      P     +   K   ++  + V + C A       I W+K  E I +                 E   D+   G L  N+L I  ++  D   Y+C A N  G  + +  V   + P    D   +D+E
Sbjct: 1837 IFRESETITLDCPVSLGDFQITWMKQGLPLTENDAIFTLDNTRLTILNAN---------------RDH----EDIYTCVANNTAGQVSKDFDVV-VQVLPKIKNAV---VTLEINEGEEIILTCDAEGNPTPTAKWDFNQG-DLPKEAVFVNNNHTVVVNNVTKYHTGVYKCYATNKVGQAVKTINVHVRTKPRFESGLTESELTVNLTRSITLECDV-DDAIGVGISWTVNGKPFLAETDGVQTLAGGRFLHIVSAKTDDHGSYACTVTN---------EAGVATKTFNLFVQVPPTIVNEGGE--YTVIENNSLVLPCEVT----GKPNPVVTWTKDGRPVGDLKSVQVLSEGQQFKIVHAEIAHKGSYICMAKNDVGTAEISFDVDIIT---RPMIQKGIKNIVTAIKGGALP----FKCPIDDDKNFKGQII----WLRNYQPI-----DLEAEDARITRLSNDR------RLTI----------LNVTENDEGQYSCRVKNDAGENSFD-FKATVLVPPTIIMLDKDKNKTAVEHSTVTLSCPATGKPEPDITWFKDGEAIHI-----------------ENIADIIPNGELNGNQLKITRIKEGDAGKYTCEADNSAGSVEQDVNVNVITIPKIEKDGIPSDYE 2396          

HSP 7 Score: 76.2554 bits (186), Expect = 1.840e-13
Identity = 164/698 (23.50%), Postives = 263/698 (37.68%), Query Frame = 3
Query:  363 LTIHNISSDNSGYYVCRVTLLTTSEHIISLA--VVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQWVIMQGLQIIDSSKFIA--------KIRPDMDGPLICEAKNYLGSTKETVQLNVLRDP-LIHIDSN-YRVPKNSNYSINCRVTSNPPPEFIKWY----------KLSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPK--EPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKW----IDVETKD----IVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFR-----GQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRK---IAASLNQNVVMIRCQAES----------ISTMHIK-WYKTNEHIVL-------PFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNF 2282
            L I N +  + G Y C    + ++E   ++A  ++ V T P   +F  +++     E E   I+C + G  P P IS ++          II S   ++       + + +    +  G   C A N  G   ++  + N+  KP I   +  T  A+    I L C V  NP  T+ W         DS   I          +  +  G   C A N  G+        V+  P  +    N Y V +N   +++C VTS P P  I W+          + S   D S + I + AKL       S  G Y C   N         +   S+  + L +   PK  +  I+GN +  + +     ++C       G P P + W    +D+   D    +  + ETF + N +    G+Y C A N  GK+ S + +L  +  +P     H  +       D   +I+ TC  +        + +  W          ++D +  D     N  IS    KLTI         Q SL ++  G Y C A NR G ASL++ + I    +     R  +   IA  +   V   R  ++S          IS   ++     N+  ++       PF S K+     EVT  E  R +   G     L I   ++D    +SCVA ND G+K++  
Sbjct: 3264 LHIRNATRADEGKYSC----IASNEAGTAVADFLIDVFTKP---TFETHETTFNIVEGESAKIECKIDGH-PKPTIS-WLKGGRPFNMDNIILSPRGDT-------LMILKAQRFDG-GLYTCVATNSYGDSEQDFKV-NVYTKPYIDETIDQTPKAVAGGEIILKCPVLGNPTPTVTWKRGDDAVPNDSRHTIVNNYDLKINSVTTEDAGQYSCIAVNEAGNLTTHYAAEVIGKPTFVRKGGNLYEVIENDTITMDCGVTSRPLPS-ISWFRGDKPVYLYDRYSISPDGSHITI-NKAKL-------SDGGKYICRASN---------EAGTSDIDLILKILVPPKIDKSNIIGNPLAIVAR--TIYLECPIS----GIPQPDVIWTKNGMDINMTDSRVILAQNNETFGIENVQVTDQGRYTCTATNRGGKA-SHDFSLDVL--SPPEFDIHGTQPTIKREGD---TITLTCPIKLAEDIADQVMDVSW----------TKDSRALDGDLTDNVDISDDGRKLTI--------SQASLENA--GLYTCIALNRAGEASLEFKVEILCTSLATVKCREHENNYIARHMPHVVTKPREMSQSANAYPPPVIDISRNDVQPQVAVNQPTIMRCAVTGHPFPSIKWLKNGKEVTDDENIR-IVEQG---QVLQILRTDSDHAGKWSCVAENDAGVKELEM 3889          

HSP 8 Score: 75.485 bits (184), Expect = 3.262e-13
Identity = 136/594 (22.90%), Postives = 221/594 (37.21%), Query Frame = 3
Query:  330 ESQITISHESRLTIHNISSDNSGYYVCRVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQ--EQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSL-LNIKYKPKI-KPFVPNTVNAMINQTIDLSCEVYANPAATIQWVIMQGLQI--IDSSKFIAK---------IRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLIHIDSNYRVPKNSNYSINCRVTSNPPPEF------IKWYKLSNMTDSSA---VLISDDA-KLNFILIDRSSTGMYACVTQN---------------IQISTTGKRKPANSEKTMFLT-VTYKPKEPQILGNK----IISIMKG---------EKFIIKCSTDRKSPGEPFPTLKWI-------DVETKDIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIA 1928
            +S+ TI +   L I++++++++G Y C + +          A   +  P         K  N+++  E + I + C V+   P P IS +   D  +      S +   S I    A        L + G  +C A N+AG  T +  L L I   PKI K  +     A++ +TI L C +   P   + W    G+ I   DS   +A+         ++    G   C A N  G       L+VL  P   I       K    +I          +       + W K S   D      V ISDD  KL        + G+Y C+  N                 ++T   R+  N+     +  V  KP+E     N     +I I +          +  I++C+      G PFP++KW+       D E   IV   +  Q++ ++S H GK+ C+AEN+ G    +E  +V     P +++   +  +      L ++I+  C   G P  + +W  G   I      F   D       RIS    +L I            L+ +  G Y C+A N  G +
Sbjct: 3434 DSRHTIVNNYDLKINSVTTEDAGQYSC-IAVNEAGNLTTHYAAEVIGKP-----TFVRKGGNLYEVIENDTITMDCGVTSR-PLPSIS-WFRGDKPVYLYDRYSISPDGSHITINKA-------KLSDGGKYICRASNEAG--TSDIDLILKILVPPKIDKSNIIGNPLAIVARTIYLECPISGIPQPDVIWT-KNGMDINMTDSRVILAQNNETFGIENVQVTDQGRYTCTATNRGGKASHDFSLDVLSPPEFDIHGTQPTIKREGDTITLTCPIKLAEDIADQVMDVSWTKDSRALDGDLTDNVDISDDGRKLTISQASLENAGLYTCIALNRAGEASLEFKVEILCTSLATVKCREHENNYIARHMPHVVTKPREMSQSANAYPPPVIDISRNDVQPQVAVNQPTIMRCAVT----GHPFPSIKWLKNGKEVTDDENIRIVEQGQVLQILRTDSDHAGKWSCVAENDAG---VKELEMVLDVFTPPVVSVKSDNPI----KALGETITLFCNASGNPYPQLKWAKGGSLI------FDSPD-----GARISLKGARLDI----------PHLKKTDVGDYTCQALNAAGTS 3977          

HSP 9 Score: 71.633 bits (174), Expect = 3.909e-12
Identity = 137/614 (22.31%), Postives = 240/614 (39.09%), Query Frame = 3
Query:  318 VPDSESQITISHESRLTIHNISSDNSGYYVCRVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLE-NRGFVMCTAINDAGVLTKNTSLLNIKYKPKI-KPFVPNTVNAMINQTIDLSCEVYANPAATIQWVI--------MQGLQIIDSSKFIA--KIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLIHIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMTDSS--AVLISDDAKLNFILIDRS-STGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGN--KIISIMKGEKFIIKCSTDRKSPGEPFPTLKWI-------DVETKDIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNE 2087
            V D E+   +     L I    SD++G + C   +      +  L +V     P  +S   +  I    E   I + C+ SG  P P++     K  ++    I  S  P+     +    L  PH  + + G   C A+N AG    + S+ ++   P+I +  +  +      Q++ L C     P   ++W +          G+ +   S FI    +     G   C A+N  GS      ++V++ P+I      +V +     I C V   P P+ + W +  N  ++    V    D ++  I+  RS  +G+Y C       S T +   A    T+ + V+     P+I+ +   +++   G KF + C+      G P P + W        D E    +    T  +  +E RH   Y+C A+N+ G   + E  + T+  AP I T   NR  +++ S+  +++   CE   +    +  + G   +P ++  F  ED++ I                      +  S R S  G Y+C A N+ G A+    L +   P I    R + +      + V + C+A  +    I WYK NE
Sbjct: 3846 VTDDENIRIVEQGQVLQILRTDSDHAGKWSC---VAENDAGVKELEMVLDVFTPPVVSVKSDNPIKALGET--ITLFCNASGN-PYPQLKW--AKGGSL----IFDS--PDGARISLKGARLDIPHLKKTDVGDYTCQALNAAGTSEASVSV-DVLVPPEINRDGIDMSPRLPAQQSLTLQCLAQGKPVPQMRWTLNGTALTHSTPGITVASDSTFIQINNVSLSDKGVYTCYAENVAGSDNLMYNVDVVQAPVISNGGTKQVIEGELAVIECLVEGYPAPQ-VSWLRNGNRVETGVQGVRYVTDGRMLTIIEARSLDSGIYLC-------SATNEAGSAQQAYTLEVLVS-----PKIITSTPGVLTPSSGSKFSLPCAVR----GYPDPIISWTLNGNDIKDGENGHTIGADGTLHIEKAEERHL-IYECTAKNDAGAD-TLEFPVQTI-VAPKISTS-GNR--YINGSEGTETV-IKCEIESESSEFSWSKNGVPLLPSNNLIFS-EDYKLI----------------------KILSTRLSDQGEYSCTAANKAGNATQKTNLNVGVAPKIMERPRTQVVHKG---DQVTLWCEASGVPQPAITWYKDNE 4394          

HSP 10 Score: 71.633 bits (174), Expect = 4.825e-12
Identity = 118/555 (21.26%), Postives = 210/555 (37.84%), Query Frame = 3
Query:  705 NRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQWVIMQGLQIIDSSKFIAKIRPD---------MDGPL-ICEAKNYLGSTKETVQLNVLRDPLIH--IDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMT--DSSAVLISD-DAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETK-------DIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQ---AESIS--TMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEV 2288
            + G   C A N+AG    +  L+++  KP  +     T N +  ++  + C++  +P  TI W  ++G +  +    I   R D          DG L  C A N  G +++  ++NV   P I   ID   +        + C V  NP P  + W +  +    DS   ++++ D K+N +  + +  G Y+C+  N         +  N        V  KP   +  GN +  +++ +   + C    +    P P++ W   +          I  D     +  ++    GKY C A NE G S   +  L+     P  I   K+  +    + + ++I   C   G P  +  W         +  D  + D R I  +              + TF  + +++ +  GRY C A NR G AS D+ L        +++I   +       + + + C    AE I+   M + W            +K     + ++T      D+   G K   LTI     ++   Y+C+A N  G   + F+V
Sbjct: 3273 DEGKYSCIASNEAGTAVADF-LIDVFTKPTFETH-ETTFNIVEGESAKIECKIDGHPKPTISW--LKGGRPFNMDNIILSPRGDTLMILKAQRFDGGLYTCVATNSYGDSEQDFKVNVYTKPYIDETIDQTPKAVAGGEIILKCPVLGNPTPT-VTWKRGDDAVPNDSRHTIVNNYDLKINSVTTEDA--GQYSCIAVN---------EAGNLTTHYAAEVIGKPTFVRKGGN-LYEVIENDTITMDCGVTSR----PLPSISWFRGDKPVYLYDRYSISPDGSHITINKAKLSDGGKYICRASNEAGTS---DIDLILKILVPPKI--DKSNIIGNPLAIVARTIYLECPISGIPQPDVIWTK-------NGMDINMTDSRVILAQN-------------NETFGIE-NVQVTDQGRYTCTATNRGGKASHDFSL--DVLSPPEFDIHGTQPTIKREGDTITLTCPIKLAEDIADQVMDVSW------------TKDSRALDGDLTD---NVDISDDGRK---LTISQASLENAGLYTCIALNRAGEASLEFKV 3760          

HSP 11 Score: 70.0922 bits (170), Expect = 1.120e-11
Identity = 144/689 (20.90%), Postives = 249/689 (36.14%), Query Frame = 3
Query:  330 ESQITISHESRLTIHNISSDNSGYYVCRVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIK-PFVPNTVNAMINQTIDLSCEVYANPAATIQWVIMQGLQIIDSSKF----------IAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLIHIDSNYR----VPKNSNYSINCRVTSNPPPEFIKWYKLSNMTD-----SSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKW-IDVETKD------IVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQ--HKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQI-FTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQP 2306
            E + ++ +   L I+    ++ G Y C VT    +  + +   V V+ PP  I     ++ N     + + ++C V    PP  ++ +        R  I         + E     + +   +E+     C A N AG    N  +  I   P IK P V    +   +    L C V++NP   I W +     I D + +          + K +    G   C A+N  G   ++ Q+ V+  PL   +S Y+      +    ++ C V+  P P+ I W     + +       A L +D   L+F  +     G Y CV Q     + G     + E ++          P +  +  + +  G+   + C  D ++  +   T  W I+    D      I SD     + +++  +NGKY C   N  GK+    T  + V E P  +      N+K+  +       I   C+  G P     W+     +          D   + +E +S +   KLT                  + + +C A+N+ G AS D+F+     P  K       I        + + C   S+    I W K      LP  ++  +IF  + T+                LTI +   D    Y+CVA+N  G    +F+VV +  P
Sbjct: 1315 EKKFSVDNLGTLRIYEADKNDIGNYNCVVTNEAGTSQMTTH--VDVQEPP--IILPSTQTNNTAVVGDRVELKCYVEAS-PPASVTWF--------RRGIAIGTDTKGYVVESDGTLVIQSASVEDATIYTCKASNPAGKAEANLQVTVIA-SPDIKDPDVVTQESIKESHPFSLYCPVFSNPLPQISWYLNDKPLIDDKTSWKTSDDKRKLHVFKAKITDSGVYKCVARNAAGEGSKSFQVEVIV-PLNLDESKYKKKVFAKEGEEVTLGCPVSGFPVPQ-INWVVDGTVVEPGKKYKGATLSNDGLTLHFDSVSVKQEGNYHCVAQ-----SKGNILDIDVELSVLAV-------PIVGEDDNLEVFLGKDISLSC--DLQTESDDKTTFVWSINGSESDRPDNVQIPSDGHRLYITDAKPENNGKYMCRVTNSAGKA--ERTLTLDVLEPPVFVEPVFEANQKLIGN-----NPIILQCQVTGNPKPTVIWKIDGNDV----------DKSWLFDESLSLLRIEKLT----------------GKSAQISCTAENKAGTASRDFFIQNIAAPTFKNEGDQETIFRE--SETITLDCPV-SLGDFQITWMKQG----LPL-TENDAIFTLDNTR----------------LTILNANRDHEDIYTCVANNTAGQVSKDFDVVVQVLP 1916          

HSP 12 Score: 67.3958 bits (163), Expect = 7.957e-11
Identity = 115/559 (20.57%), Postives = 209/559 (37.39%), Query Frame = 3
Query:  702 ENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQWVI----MQGLQIIDSSKFIAKIRP--DMDGPLICEAKNYLGSTKETVQLNVLRDPLIHIDSNYRV--PKNSNYSINCRVTSNPPPEF-IKWYKLS-NMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIIS--IMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETKDIVSDM------ETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTN------EHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQP 2306
            EN G  MC   N AG   +  +L  ++    ++P        + N  I L C+V  NP  T+ W I    +    + D S  + +I         + C A+N  G+      +  +  P    + +      ++   +++C V+     +F I W K    +T++ A+   D+ +L  +  +R    +Y CV  N    T G+       K   + V   PK    + N +++  I +GE+ I+ C  +    G P PT KW D    D+  +        T  V N    H G YKC A N++G++   +T  V V   P   +     ++ +   +L +SI+  C+          W         + + F  E              T     L    F    S ++  +G Y C   N  G+A+  + L ++ PP I        +   +  N +++ C+        + W K        + + +  + +++ I  AE+                              +Y C+A ND G  +++F+V   ++P
Sbjct: 1708 ENNGKYMCRVTNSAGKAERTLTLDVLEPPVFVEPVFEANQKLIGNNPIILQCQVTGNPKPTVIWKIDGNDVDKSWLFDESLSLLRIEKLTGKSAQISCTAENKAGTASRDFFIQNIAAPTFKNEGDQETIFRESETITLDCPVSLG---DFQITWMKQGLPLTENDAIFTLDNTRLTILNANRDHEDIYTCVANN----TAGQ-----VSKDFDVVVQVLPK----IKNAVVTLEINEGEEIILTCDAE----GNPTPTAKW-DFNQGDLPKEAVFVNNNHTVVVNNVTKYHTGVYKCYATNKVGQAV--KTINVHVRTKPRFESGLTESELTV---NLTRSITLECDVDDAIGVGISWTV-------NGKPFLAE--------------TDGVQTLAGGRFLHIVSAKTDDHGSYACTVTNEAGVATKTFNLFVQVPPTIVNEGGEYTV---IENNSLVLPCEVTGKPNPVVTWTKDGRPVGDLKSVQVLSEGQQFKIVHAEIAHK--------------------------GSYICMAKNDVGTAEISFDVDIITRP 2190          

HSP 13 Score: 63.929 bits (154), Expect = 1.081e-9
Identity = 127/631 (20.13%), Postives = 239/631 (37.88%), Query Frame = 3
Query:  525 EQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIK-----YKPKIKPFVPNTVNAM-------INQTID--------LSCEVYANPAATIQWVIMQGLQII----------DSSKFIAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLIH-IDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETKDI---VSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNR-KVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHR-NISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQPD 2309
            E + + + C V  G P P I ++I  D  ++         P   I    ++   R    ++ G   C A++ AG  T + ++  IK     YKP+       T++ M       +N T D        + C V   P   I W  + G  I           D++  + K      G   C+A N  G  ++   + ++  P+I    S++ +  +  ++I C V  +P P  I W  L +    +  ++++D  L    ++ +  G + C  QN            N  +T+ LTV   P        KI   ++ +  +++C      P     T +   ++++ I   + +     + N +  + G + C   N  G+     T  +TV+E P II++      V   ++     I   C   G P     W         +  D K+++ + ++ N    +I+     D+                G YNC   N  G + +   + ++ PPII  + +    A   ++  V ++C  E+     + W++    I +   +K Y + E++ T                 L I+    +D + Y+C ASN  G  + N +V   + PD
Sbjct:  900 EGQDLTLSCVVVLGTPKPSI-VWIKDDKPVEE-------GPTIKIEGGGSLLRLRGGNPKDEGKYTCIAVSPAGNSTLHINVQLIKKPEFVYKPEGGIVFKPTISGMDEKHVAVVNSTHDVLDGEGFAIPCVVSGTPPPIITWY-LDGRPITPNSRDFTVTADNTLIVRKADKSYSGVYTCQATNSAGDNEQKTTIRIMNTPMISPGQSSFNMVVDDLFTIPCDVYGDPKP-VITW--LLDDKPFTEGVVNEDGSLTIPNVNEAHRGTFTCHAQNAA---------GNDTRTVTLTVHTTPTINAENQEKIA--LQNDDIVLECPAKALPPPVRLWTYEGEKIDSQLIPHTIREDGALVLQNVKLENTGVFVCQVSNLAGEDSLSYT--LTVHEKPKIISEVPGVVDVVKGFT-----IEIPCRATGVPEVIRTWNK-------NGIDLKMDEKKFSVDNLGTLRIYEADKNDI----------------GNYNCVVTNEAGTSQMTTHVDVQEPPIILPSTQTNNTAVVGDR--VELKCYVEASPPASVTWFRRG--IAIGTDTKGY-VVESDGT-----------------LVIQSASVEDATIYTCKASNPAGKAEANLQVTVIASPD 1455          

HSP 14 Score: 61.6178 bits (148), Expect = 4.958e-9
Identity = 132/640 (20.62%), Postives = 233/640 (36.41%), Query Frame = 3
Query:  153 ITPNNATVNSKENLTLECVVDQQ-NIVYSWEY--KPFHIKSNIMSKVLGFCYFEKPCVVPDSESQITISHESRLTIHNISSDNSGYYVCRVTLLTTSEHIISLAV--VTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSL-LNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQWVIMQGLQIIDSSKFIAKI--------------RPDMDGPLICEAKNYLGSTKETVQLNVLRDPLIHI---DSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMTDSSAVLISDDA-----------KLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSP--------GEPFPTLKWIDVETKDIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFL 1946
            +T +  TVN   ++TLEC VD    +  SW    KPF  +++ +  + G  +                     L I +  +D+ G Y C V    T+E  ++     + V+ PP  ++     ++    E   + + C V+G   P  +  +      +  LK +        ++E     +     + ++G  +C A ND G  T   S  ++I  +P I+  + N V A+    +   C +  +     Q + ++  Q ID     A+I                + +G   C  KN  G      +  VL  P I +   D N    ++S  +++C  T  P P+ I W+K     D  A+ I + A           +L    I     G Y C   N            + E+ + + V   PK  +          + E+ +I C    + P        G+P  + K+  V+T      +  F++  ++S    KY C+A NE G    +    V++  APS    +  R++ ++  + P ++   C  +G P     W      I P+ +    +  R +                      Q +    S  GRY C A N  G   L+  L
Sbjct: 2011 LTESELTVNLTRSITLECDVDDAIGVGISWTVNGKPFLAETDGVQTLAGGRF---------------------LHIVSAKTDDHGSYACTV----TNEAGVATKTFNLFVQVPPTIVNEGGEYTV---IENNSLVLPCEVTG--KPNPVVTWTKDGRPVGDLKSV------QVLSEGQQFKIVHAE-IAHKGSYICMAKNDVG--TAEISFDVDIITRPMIQKGIKNIVTAIKGGALPFKCPIDDDKNFKGQIIWLRNYQPIDLEAEDARITRLSNDRRLTILNVTENDEGQYSCRVKNDAGENSFDFKATVLVPPTIIMLDKDKNKTAVEHSTVTLSCPATGKPEPD-ITWFK-----DGEAIHIENIADIIPNGELNGNQLKITRIKEGDAGKYTCEADN---------SAGSVEQDVNVNVITIPKIEKDGIPSDYESQQNERVVISCPVYARPPAKITWLKAGKPLQSDKF--VKTSANGQKLYLFKLRETDSS---KYTCIATNEAGT--DKRDFKVSMLVAPSFDEPNIVRRITVNSGN-PSTLH--CPAKGSPSPTITWLKDGNAIEPNDRYVFFDAGRQL----------------------QISKTEGSDQGRYTCIATNSVGSDDLENTL 2564          

HSP 15 Score: 55.4546 bits (132), Expect = 3.357e-7
Identity = 65/315 (20.63%), Postives = 121/315 (38.41%), Query Frame = 3
Query:  357 SRLTIHNISSDNSGYYVCRVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINV-FQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYL--ENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQWVIMQ-------GLQIIDSSKF--IAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLIHIDSN-YRVPKNSNYSINCRVTSNPPPEFIKWYK-LSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNI 1259
            S  TI  +S  ++G Y CRV +     + +   V T ++PP+       ++++V   E  F+      +G +              I+  +  ++ F              + H++   + G   C A N  G+ T+   L +I   P +K   P  V   + + ++LSCE   +P   + W             Q+   SKF  I       +G   C A +  G  ++T  L +   P + I  N   V +    S  C+     P   I+W+K   ++      +  ++ +L+ +       G Y+CV +N+
Sbjct:  482 SVWTIPELSLKDAGEYECRV-ISNNGNYSVKTRVETRESPPEIFGV---RNVSVPLGEAAFLHCSTRSAGEV-------------EIRWTRYGATVFNGPNTERNPTNGTLKIHHVTRADAGVYECMARNAGGMSTRKMRL-DIMEPPSVK-VTPQDVYFNMREGVNLSCEAMGDPKPEVHWYFKGRHLLNDYKYQVGQDSKFLYIRDATHHDEGTYECRAMSQAGQARDTTDLMLATPPKVEIIQNKMMVGRGDRVSFECKTIRGKPHPKIRWFKNGKDLIKPDDYIKINEGQLHIMGAKDEDAGAYSCVGENM 777          

HSP 16 Score: 54.6842 bits (130), Expect = 5.656e-7
Identity = 96/423 (22.70%), Postives = 164/423 (38.77%), Query Frame = 3
Query:  516 VFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMIN--QTIDLSCEVYANPAATIQWVIMQGL--------QIIDSSKFIAKIRPDMDGPLICEAKNYLGSTKETVQLNV----LRDPLIHIDSNYRV-PKNSNYSINCRVTSNPPPEFIKWYKLSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQ-ILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWID----VETKDIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNE----APSIITQHKNRKVFLSYS-DLPQSISFTCEFRGQPL 1709
            V  + + + + C  SG +P P I+ Y  KDN     +++++   + T        ++        G   C A N      ++  L+ +   P++   VP  +N   N  QT+ LSC     P   I W+    +        QI+ ++  I  + PD DG   C AK+  G    T +L V     R   I ++ + +  PK + Y I+   T +  P+ + W  +            +D+ LN  +        Y C+         G R    S +T+ L        PQ I+  K  +   G    ++CS    + G P PT+ W      +E           +V  + +  +G+Y C+A+N +G S    +A + V+      P   +  KN  V   Y  +   S   T E+ G P+
Sbjct: 4366 VVHKGDQVTLWCEASG-VPQPAITWY--KDN-----ELLTNTGVDETATTKKKSVIFSSISPSQAGVYTCKAENWVASTEEDIDLI-VMIPPEV---VPERMNVSTNPRQTVFLSCNATGIPEPVISWMRDSNIAIQNNEKYQILGTTLAIRNVLPDDDGFYHCIAKSDAGQKIATRKLIVNKPSDRPAPIWVECDEKGKPKKTEYMIDRGDTPDDNPQLLPWKDV------------EDSSLNGSI-------AYRCM--------PGPR----SSRTVLLHAA-----PQFIVKPKNTTAAIGAIVELRCS----AAGPPHPTITWAKDGKLIEDSKFEIAYSHLKVTLNSTSDSGEYTCMAQNSVGSSTV--SAFINVDNNILPTPKPSSNQKNVAVITCYERNQAYSRGLTWEYNGVPM 4734          
BLAST of hemicentin-1 isoform X2 vs. Ensembl Celegans
Match: him-4 (High Incidence of Males (Increased X chromosome loss) [Source:UniProtKB/TrEMBL;Acc:A4UVK0])

HSP 1 Score: 104.375 bits (259), Expect = 5.534e-22
Identity = 157/706 (22.24%), Postives = 278/706 (39.38%), Query Frame = 3
Query:  306 KPCVVPDSESQIT-ISHESRLTIHNISSDNSGYYVCRVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLE-NRGFVMCTAINDAGVLTKNTSLLNIKYKPKI-KPFVPNTVNAMINQTIDLSCEVYANPAATIQWVIMQGLQIIDSSKFIA-----------KIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLI---HIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMTDSSAVLISDDA--KLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVE-TKD----------------IVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQ--HKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAE-SISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQP 2306
            +P  +   +++IT +S++ RLTI N++ ++ G Y CRV            A V V  PP  I    +K+     E   + + C  +G  P P I+ +  KD     ++ I+   PN    E++   L      E + G   C A N AG + ++ ++ N+   PKI K  +P+   +  N+ + +SC VYA P A I W  ++  + + S KF+            K+R        C A N  G+ K   ++++L  P     +I     V   +  +++C    +P P  I W K  N  + +   +  DA  +L     + S  G Y C+  N    + G     N+ + +          P I G +  ++   E F  +   D  S G        +DVE  KD                I S  +    +++    +G+Y C+  N  G+  +R+     VN+ PSI  +    N +  + Y      +   C   G P  +  W + ++ + P S  +++ ++        SQ+                        G Y CEA N  G A  D+ + +  PP  +   + R+   +   + +++ C AE S+    + W+  +E +     + KY+  E                     L + +++ DD   Y C A N+ GI K  F+++    P
Sbjct: 2231 QPIDLEAEDARITRLSNDRRLTILNVTENDEGQYSCRVKNDAGENSFDFKATVLV--PPTIIMLDKDKNKTAV-EHSTVTLSCPATGK-PEPDITWF--KDGEAIHIENIADIIPN---GELNGNQLKITRIKEGDAGKYTCEADNSAGSVEQDVNV-NVITIPKIEKDGIPSDYESQQNERVVISCPVYARPPAKITW--LKAGKPLQSDKFVKTSANGQKLYLFKLRETDSSKYTCIATNEAGTDKRDFKVSMLVAPSFDEPNIVRRITVNSGNPSTLHCPAKGSPSPT-ITWLKDGNAIEPNDRYVFFDAGRQLQISKTEGSDQGRYTCIATN----SVGSDDLENTLEVII--------PPVIDGERREAVAVIEGFSSELFCDSNSTG--------VDVEWQKDGLTINQDTLRGDSFIQIPSSGKKMSFLSARKSDSGRYTCIVRNPAGE--ARKLFDFAVNDPPSISDELSSANIQTIVPY----YPVEINCVVSGSPHPKVYWLFDDKPLEPDSAAYELTNNGETLKIVRSQV---------------------EHAGTYTCEAQNNVGKARKDFLVRVTAPPHFE---KEREEVVARVGDTMLLTCNAESSVPLSSVYWHAHDESVQNGVITSKYAANE-------------------KTLNVTNIQLDDEGFYYCTAVNEAGITKKFFKLIVIETP 2854          

HSP 2 Score: 94.3597 bits (233), Expect = 5.416e-19
Identity = 148/706 (20.96%), Postives = 273/706 (38.67%), Query Frame = 3
Query:  306 KPCVVPDSESQITISHESRLTIHNISSDNSGYYVCRVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLW-RPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEV-YANPAATIQWV-----IMQGLQI-IDSSKFIAKIR---PDMDGPLICEAKNYLGSTKETVQLNVLRDP-----------------------LIHIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSN--MTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVE---TKDIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQP 2306
            K  + PD   +I   +E +L I     +++G Y C    +   +  + +A ++V   P  I       +N+ ++   + +QC ++ GIPPP+I          ++  ++ +   N    +++  +L      +E++G   C A N  G  +++T+L+       +   VP     +  Q + LSC V    P  +I W+     + +G  I I+    + ++R   P  +G   C A +  G++   + + +++ P                       +  ++S + V     ++I C V+  PPP  I WY        +S    ++ D  L     D+S +G+Y C   N    + G     N +KT    +      P   G    +++  + F I C       G+P P + W+  +   T+ +V++  +  + N    H G + C A+N  G      T  +TV+  P+I  +++  K+ L   D    I   C  +  P     W Y  EKI     D ++  H               TI        Q   L ++  G + C+  N  G  SL Y L +   P I   +    +   +    + I C+A  +  +   W K    I L    KK+S+                    +  L I + + +D  NY+CV +N+ G  ++   V  +  P
Sbjct:  741 KDLIKPDDYIKI---NEGQLHIMGAKDEDAGAYSCVGENMAGKD--VQVANLSVGRVPTIIESPHTVRVNIERQ---VTLQC-LAVGIPPPEIEW--------QKGNVLLATLNNPRYTQLADGNLLITDAQIEDQGQFTCIARNTYGQQSQSTTLMVTGLVSPVLGHVPPEEQLIEGQDLTLSCVVVLGTPKPSIVWIKDDKPVEEGPTIKIEGGGSLLRLRGGNPKDEGKYTCIAVSPAGNSTLHINVQLIKKPEFVYKPEGGIVFKPTISGMDEKHVAVVNSTHDVLDGEGFAIPCVVSGTPPP-IITWYLDGRPITPNSRDFTVTADNTLIVRKADKSYSGVYTCQATN----SAGD----NEQKTTIRIMNTPMISP---GQSSFNMVVDDLFTIPCDVY----GDPKPVITWLLDDKPFTEGVVNEDGSLTIPNVNEAHRGTFTCHAQNAAGND--TRTVTLTVHTTPTINAENQ-EKIALQNDD----IVLECPAKALPPPVRLWTYEGEKI-----DSQLIPH---------------TIREDGALVLQNVKLENT--GVFVCQVSNLAGEDSLSYTLTVHEKPKIISEVP--GVVDVVKGFTIEIPCRATGVPEVIRTWNKNG--IDLKMDEKKFSVD------------------NLGTLRIYEADKNDIGNYNCVVTNEAGTSQMTTHVDVQEPP 1362          

HSP 3 Score: 85.5001 bits (210), Expect = 2.670e-16
Identity = 152/735 (20.68%), Postives = 273/735 (37.14%), Query Frame = 3
Query:  165 NATVNSKENLTLECVVDQQNIVYSWEYKPFHIKSNIMSKVLGFCYFEKPCVVPDSESQITISHESRLTIHNISSDNSGYYVC-RVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENR-GFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQWVIMQGLQIIDSSKFIAKI-----RPDM---DGPLICEAKNYLGSTKETVQLNVLRDPLIHIDSNYRVPKNSN-YSINCRVTSNPPPEFIKWYKLSNMTDSSAVLISDDAKLNFILIDRSST----GMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWI------DVETKDIVSDMETFQVINSESRHNGKYKCLAENEIGKS--------FSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNF 2282
            N  V + + LTLEC ++  + V+    + F     +                 D  +Q + S +S+L I   + +++  Y C  V     +E +     VTV TPPK     F  S         + I C   G IPPP+IS ++     ++         P  T  +   +SL   +   N+ G   C A N AG   ++T  + I   P++       +  +  +   + CEV+ NP   + W+        D  +F  K+     R       G   C A N  G ++ T  + VL  P I  +      K  N Y ++C+VT  P P ++ W +     +          + N +L  R++T    G Y+C+  N   +       A+    +F   T++  E         +I++GE   I+C  D    G P PT+ W+      +++   +    +T  ++ ++    G Y C+A N  G S        +++     T+++ P  +   +              I   C   G P     W+ G++ +P  S+      H  ++N            DL      +  S+ +   G+Y+C A N  G  +  Y   +   P      +   +   +  + + + C   S     I W++ ++ + L     +YSI         P+          + +TI   +  D   Y C ASN+ G   ++ 
Sbjct: 2951 NVEVQAGDPLTLECPIEDTSGVHITWSRQFGKDGQL-----------------DMRAQSS-SDKSKLYIMQATPEDADSYSCIAVNDAGGAEAVFQ---VTVNTPPKIFGDSF--STTEIVADTTLEIPCRTEG-IPPPEISWFLDGKPILE--------MPGVTYKQ-GDLSLRIDNIKPNQEGRYTCVAENKAGRAEQDT-YVEISEPPRVV-MASEVMRVVEGRQTTIRCEVFGNPEPVVNWLKDGEPYTSDLLQFSTKLSYLHLRETTLADGGTYTCIATNKAGESQTTTDVEVLVPPRIEDEERVLQGKEGNTYMVHCQVTGRPVP-YVTWKRNGKEIE----------QFNPVLHIRNATRADEGKYSCIASNEAGTAV-----ADFLIDVFTKPTFETHE------TTFNIVEGESAKIECKID----GHPKPTISWLKGGRPFNMDNIILSPRGDTLMILKAQRFDGGLYTCVATNSYGDSEQDFKVNVYTKPYIDETIDQTPKAVAGGE--------------IILKCPVLGNPTPTVTWKRGDDAVPNDSR------HTIVNN-----------YDL------KINSVTTEDAGQYSCIAVNEAGNLTTHYAAEVIGKPTFVR--KGGNLYEVIENDTITMDCGVTSRPLPSISWFRGDKPVYL---YDRYSI--------SPDG---------SHITINKAKLSDGGKYICRASNEAGTSDIDL 3565          

HSP 4 Score: 84.3445 bits (207), Expect = 5.239e-16
Identity = 159/677 (23.49%), Postives = 256/677 (37.81%), Query Frame = 3
Query:  363 LTIHNISSDNSGYYVCRVTLLTTSEHIISLA--VVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQWVIMQGLQIIDSSKFIA--------KIRPDMDGPLICEAKNYLGSTKETVQLNVLRDP-LIHIDSN-YRVPKNSNYSINCRVTSNPPPEFIKWY----------KLSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPK--EPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKW----IDVETKD----IVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFR-----GQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNF 2282
            L I N +  + G Y C    + ++E   ++A  ++ V T P   +F  +++     E E   I+C + G  P P IS ++          II S   ++       + + +    +  G   C A N  G   ++  + N+  KP I   +  T  A+    I L C V  NP  T+ W         DS   I          +  +  G   C A N  G+        V+  P  +    N Y V +N   +++C VTS P P  I W+          + S   D S + I + AKL       S  G Y C   N         +   S+  + L +   PK  +  I+GN +  + +     ++C       G P P + W    +D+   D    +  + ETF + N +    G+Y C A N  GK+ S + +L  +  +P     H  +       D   +I+ TC  +        + +  W          ++D +  D     N  IS    KLTI         Q SL ++  G Y C A NR G ASL++ + I  PP+I  +    +   ++NQ  +M RC         IKW K  + +      +   I E                 +   L I   ++D    +SCVA ND G+K++  
Sbjct: 3264 LHIRNATRADEGKYSC----IASNEAGTAVADFLIDVFTKP---TFETHETTFNIVEGESAKIECKIDGH-PKPTIS-WLKGGRPFNMDNIILSPRGDT-------LMILKAQRFDG-GLYTCVATNSYGDSEQDFKV-NVYTKPYIDETIDQTPKAVAGGEIILKCPVLGNPTPTVTWKRGDDAVPNDSRHTIVNNYDLKINSVTTEDAGQYSCIAVNEAGNLTTHYAAEVIGKPTFVRKGGNLYEVIENDTITMDCGVTSRPLPS-ISWFRGDKPVYLYDRYSISPDGSHITI-NKAKL-------SDGGKYICRASN---------EAGTSDIDLILKILVPPKIDKSNIIGNPLAIVAR--TIYLECPIS----GIPQPDVIWTKNGMDINMTDSRVILAQNNETFGIENVQVTDQGRYTCTATNRGGKA-SHDFSLDVL--SPPEFDIHGTQPTIKREGD---TITLTCPIKLAEDIADQVMDVSW----------TKDSRALDGDLTDNVDISDDGRKLTI--------SQASLENA--GLYTCIALNRAGEASLEFKVEILSPPVIDISRNDVQPQVAVNQPTIM-RCAVTGHPFPSIKWLKNGKEVT---DDENIRIVE-----------------QGQVLQILRTDSDHAGKWSCVAENDAGVKELEM 3851          

HSP 5 Score: 83.9593 bits (206), Expect = 8.256e-16
Identity = 145/704 (20.60%), Postives = 267/704 (37.93%), Query Frame = 3
Query:  291 FCYFEKPCVVPDSESQITISHESRLTIHNISSDNSGYYVCRVTLLTTSEHIISLA----VVTVKTPPKSISFHFNKSIN--VFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKY-----KPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQWVI-------MQGLQIIDSSKFIAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLIHID-SNYRVPKNSNYSINCRV--TSNPPPEFIKWYKLSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKI--ISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETKDIV---------SDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQP 2306
            +  F+   + PDS +    ++   L I     +++G Y C       +++ +  A    +V V  PP     HF K     V +  + + + C+    +P   +  +   D +++   I S    N     V+ I L      ++ GF  CTA+N+AG+  K   L+ I+      + K+ P +       + + + L C     P  TI ++           + II S+  I   + +++G   C A+N  G +++ + + VL  P +  +  N  V      ++ C +  TS     + + +      D  A   SD +KL  +         Y+C+  N           A   + +F      P  P+I G+      I+      I C T+    G P P + W  ++ K I+          D+ + ++ N +    G+Y C+AEN+ G++   +   V ++E P ++   +  +V        +  +  CE  G P     W    E   P + D             + Q  TKL+  L+ R    +T+L  +  G Y C A N+ G +     + +  PP I+      ++      N  M+ CQ       ++ W +  + I                       + F P L      I++    D   YSC+ASN+ G    +F +   ++P
Sbjct: 2701 YWLFDDKPLEPDSAAYELTNNGETLKIVRSQVEHAGTYTCE------AQNNVGKARKDFLVRVTAPP-----HFEKEREEVVARVGDTMLLTCNAESSVPLSSVYWH-AHDESVQNGVITSKYAANEKTLNVTNIQL------DDEGFYYCTAVNEAGITKKFFKLIVIETPYFLDQQKLYPII-------LGKRLTLDCSATGTPPPTILFMKDGKRLNESDEVDIIGSTLVIDNPQKEVEGRYTCIAENKAGRSEKDMMVEVLLPPKLSKEWINVEVQAGDPLTLECPIEDTSGVHITWSRQFGKDGQLDMRAQSSSDKSKLYIMQATPEDADSYSCIAVN----------DAGGAEAVFQVTVNTP--PKIFGDSFSTTEIVADTTLEIPCRTE----GIPPPEISWF-LDGKPILEMPGVTYKQGDL-SLRIDNIKPNQEGRYTCVAENKAGRA--EQDTYVEISEPPRVVMASEVMRVVEG-----RQTTIRCEVFGNPEPVVNWLKDGE---PYTSD-------------LLQFSTKLSY-LHLR----ETTL--ADGGTYTCIATNKAGESQTTTDVEVLVPPRIED---EERVLQGKEGNTYMVHCQVTGRPVPYVTWKRNGKEI-----------------------EQFNPVLH-----IRNATRADEGKYSCIASNEAGTAVADFLIDVFTKP 3300          

HSP 6 Score: 80.1073 bits (196), Expect = 1.182e-14
Identity = 122/562 (21.71%), Postives = 225/562 (40.04%), Query Frame = 3
Query:  342 TISHESRLTIHNISSDNSGYYVCRVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQWVIMQGLQI-IDSSKFIAKIRPDMDGPLI-------------CEAKNYLGSTKETVQLNVLRDPLIH---IDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSN--MTDSSAVLISDDA-KLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWI----------DVETKDIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRY---GNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIA 1928
            TI  +  L + N+  +N+G +VC+V+ L   + +     +TV   PK IS      ++V +    I I C  +G   P  I  +    N I  LK+    F   ++  +  + ++      + G   C   N+AG  ++ T+ ++++  P I P       A++   ++L C V A+P A++ W   +G+ I  D+  ++     + DG L+             C+A N  G  +  +Q+ V+  P I    + +   + ++  +S+ C V SNP P+ I WY      + D ++   SDD  KL+      + +G+Y CV +N               K+  + V       +    K +   +GE+  + C       G P P + W+            +   + +D  T    +   +  G Y C+A+++ G     +  L  +  A  I+ +  N +VFL      + IS +C+ + +   +  + +   G+E   P +     + HR                 LY       T  +   NG+Y C   N  G A
Sbjct: 1227 TIREDGALVLQNVKLENTGVFVCQVSNLAGEDSL--SYTLTVHEKPKIIS-EVPGVVDVVKGFT-IEIPCRATG--VPEVIRTW--NKNGID-LKMDEKKF---SVDNLGTLRIYEADK-NDIGNYNCVVTNEAGT-SQMTTHVDVQEPPIILPSTQTNNTAVVGDRVELKCYVEASPPASVTW-FRRGIAIGTDTKGYVV----ESDGTLVIQSASVEDATIYTCKASNPAGKAEANLQVTVIASPDIKDPDVVTQESIKESHPFSLYCPVFSNPLPQ-ISWYLNDKPLIDDKTSWKTSDDKRKLHVFKAKITDSGVYKCVARNAA---------GEGSKSFQVEVIVPLNLDESKYKKKVFAKEGEEVTLGCPVS----GFPVPQINWVVDGTVVEPGKKYKGATLSNDGLTLHFDSVSVKQEGNYHCVAQSK-GNILDIDVELSVL--AVPIVGEDDNLEVFLG-----KDISLSCDLQTESDDKTTFVWSINGSESDRPDNVQIPSDGHR-----------------LYI------TDAKPENNGKYMCRVTNSAGKA 1724          

HSP 7 Score: 78.5666 bits (192), Expect = 3.274e-14
Identity = 130/565 (23.01%), Postives = 214/565 (37.88%), Query Frame = 3
Query:  330 ESQITISHESRLTIHNISSDNSGYYVCRVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQ--EQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSL-LNIKYKPKI-KPFVPNTVNAMINQTIDLSCEVYANPAATIQWVIMQGLQI--IDSSKFIAK---------IRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLIHIDSNYRVPKNSNYSINCRVTSNPPPEF------IKWYKLSNMTDSSA---VLISDDA-KLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWI-------DVETKDIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIA 1928
            +S+ TI +   L I++++++++G Y C + +          A   +  P         K  N+++  E + I + C V+   P P IS +   D  +      S +   S I    A        L + G  +C A N+AG  T +  L L I   PKI K  +     A++ +TI L C +   P   + W    G+ I   DS   +A+         ++    G   C A N  G       L+VL  P   I       K    +I          +       + W K S   D      V ISDD  KL        + G+Y C+  N     + + K         + ++    +PQ+  N        +  I++C+      G PFP++KW+       D E   IV   +  Q++ ++S H GK+ C+AEN+ G    +E  +V     P +++   +  +      L ++I+  C   G P  + +W  G   I      F   D       RIS    +L I            L+ +  G Y C+A N  G +
Sbjct: 3434 DSRHTIVNNYDLKINSVTTEDAGQYSC-IAVNEAGNLTTHYAAEVIGKP-----TFVRKGGNLYEVIENDTITMDCGVTSR-PLPSIS-WFRGDKPVYLYDRYSISPDGSHITINKA-------KLSDGGKYICRASNEAG--TSDIDLILKILVPPKIDKSNIIGNPLAIVARTIYLECPISGIPQPDVIWT-KNGMDINMTDSRVILAQNNETFGIENVQVTDQGRYTCTATNRGGKASHDFSLDVLSPPEFDIHGTQPTIKREGDTITLTCPIKLAEDIADQVMDVSWTKDSRALDGDLTDNVDISDDGRKLTISQASLENAGLYTCIALNRAGEASLEFK-VEILSPPVIDISRNDVQPQVAVN--------QPTIMRCAVT----GHPFPSIKWLKNGKEVTDDENIRIVEQGQVLQILRTDSDHAGKWSCVAENDAG---VKELEMVLDVFTPPVVSVKSDNPI----KALGETITLFCNASGNPYPQLKWAKGGSLI------FDSPD-----GARISLKGARLDI----------PHLKKTDVGDYTCQALNAAGTS 3939          

HSP 8 Score: 76.6406 bits (187), Expect = 1.197e-13
Identity = 148/650 (22.77%), Postives = 248/650 (38.15%), Query Frame = 3
Query:  516 VFQEQEFIAIQCSVSGG---IPPPKISMYIVKDNAI-----KRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQT--IDLSCEVYANPAATIQWVIMQGLQIIDSSKFIAKIRPDMD--------GPLICEAKNYLGSTKETVQLNVLRDPLIH---IDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMTDSSAVLISDDAKLNFILIDRSST---GMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWI-------DVETKDIVSDMETFQVINSESRHNGKYKCLAENEIGKS-FSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTC------EFRGQPLYEARWRYGNEKIPPSSQDFKIEDHR--NISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPG-LKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQPDPPYDLRVTDFE 2342
            +F+E E I + C VS G   I   K  + + +++AI      RL I+++N               R H         C A N AG ++K+  ++ ++  PKIK  V   V   IN+   I L+C+   NP  T +W   QG  +   + F+      +         G   C A N +G   +T+ ++V   P       +S   V    + ++ C V  +     I W        +    +   A   F+ I  + T   G YAC   N         +   + KT  L V   P      G    ++++    ++ C       G+P P + W        D+++  ++S+ + F+++++E  H G Y C+A+N++G +  S +  ++T    P I    KN    +    LP    F C       F+GQ +    W    + I     D + ED R   +SN+R      +LTI           ++  +  G+Y+C   N  G  S D F      P     +   K   ++  + V + C A       I W+K  E I +                 E   D+   G L  N+L I  ++  D   Y+C A N  G  + +  V   + P    D   +D+E
Sbjct: 1837 IFRESETITLDCPVSLGDFQITWMKQGLPLTENDAIFTLDNTRLTILNAN---------------RDH----EDIYTCVANNTAGQVSKDFDVV-VQVLPKIKNAV---VTLEINEGEEIILTCDAEGNPTPTAKWDFNQG-DLPKEAVFVNNNHTVVVNNVTKYHTGVYKCYATNKVGQAVKTINVHVRTKPRFESGLTESELTVNLTRSITLECDV-DDAIGVGISWTVNGKPFLAETDGVQTLAGGRFLHIVSAKTDDHGSYACTVTN---------EAGVATKTFNLFVQVPPTIVNEGGE--YTVIENNSLVLPCEVT----GKPNPVVTWTKDGRPVGDLKSVQVLSEGQQFKIVHAEIAHKGSYICMAKNDVGTAEISFDVDIIT---RPMIQKGIKNIVTAIKGGALP----FKCPIDDDKNFKGQII----WLRNYQPI-----DLEAEDARITRLSNDR------RLTI----------LNVTENDEGQYSCRVKNDAGENSFD-FKATVLVPPTIIMLDKDKNKTAVEHSTVTLSCPATGKPEPDITWFKDGEAIHI-----------------ENIADIIPNGELNGNQLKITRIKEGDAGKYTCEADNSAGSVEQDVNVNVITIPKIEKDGIPSDYE 2396          

HSP 9 Score: 72.0182 bits (175), Expect = 3.684e-12
Identity = 137/614 (22.31%), Postives = 240/614 (39.09%), Query Frame = 3
Query:  318 VPDSESQITISHESRLTIHNISSDNSGYYVCRVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLE-NRGFVMCTAINDAGVLTKNTSLLNIKYKPKI-KPFVPNTVNAMINQTIDLSCEVYANPAATIQWVI--------MQGLQIIDSSKFIA--KIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLIHIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMTDSS--AVLISDDAKLNFILIDRS-STGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGN--KIISIMKGEKFIIKCSTDRKSPGEPFPTLKWI-------DVETKDIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNE 2087
            V D E+   +     L I    SD++G + C   +      +  L +V     P  +S   +  I    E   I + C+ SG  P P++     K  ++    I  S  P+     +    L  PH  + + G   C A+N AG    + S+ ++   P+I +  +  +      Q++ L C     P   ++W +          G+ +   S FI    +     G   C A+N  GS      ++V++ P+I      +V +     I C V   P P+ + W +  N  ++    V    D ++  I+  RS  +G+Y C       S T +   A    T+ + V+     P+I+ +   +++   G KF + C+      G P P + W        D E    +    T  +  +E RH   Y+C A+N+ G   + E  + T+  AP I T   NR  +++ S+  +++   CE   +    +  + G   +P ++  F  ED++ I                      +  S R S  G Y+C A N+ G A+    L +   P I    R + +      + V + C+A  +    I WYK NE
Sbjct: 3808 VTDDENIRIVEQGQVLQILRTDSDHAGKWSC---VAENDAGVKELEMVLDVFTPPVVSVKSDNPIKALGET--ITLFCNASGN-PYPQLKW--AKGGSL----IFDS--PDGARISLKGARLDIPHLKKTDVGDYTCQALNAAGTSEASVSV-DVLVPPEINRDGIDMSPRLPAQQSLTLQCLAQGKPVPQMRWTLNGTALTHSTPGITVASDSTFIQINNVSLSDKGVYTCYAENVAGSDNLMYNVDVVQAPVISNGGTKQVIEGELAVIECLVEGYPAPQ-VSWLRNGNRVETGVQGVRYVTDGRMLTIIEARSLDSGIYLC-------SATNEAGSAQQAYTLEVLVS-----PKIITSTPGVLTPSSGSKFSLPCAVR----GYPDPIISWTLNGNDIKDGENGHTIGADGTLHIEKAEERHL-IYECTAKNDAGAD-TLEFPVQTI-VAPKISTS-GNR--YINGSEGTETV-IKCEIESESSEFSWSKNGVPLLPSNNLIFS-EDYKLI----------------------KILSTRLSDQGEYSCTAANKAGNATQKTNLNVGVAPKIMERPRTQVVHKG---DQVTLWCEASGVPQPAITWYKDNE 4356          

HSP 10 Score: 70.4774 bits (171), Expect = 1.110e-11
Identity = 144/689 (20.90%), Postives = 249/689 (36.14%), Query Frame = 3
Query:  330 ESQITISHESRLTIHNISSDNSGYYVCRVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIK-PFVPNTVNAMINQTIDLSCEVYANPAATIQWVIMQGLQIIDSSKF----------IAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLIHIDSNYR----VPKNSNYSINCRVTSNPPPEFIKWYKLSNMTD-----SSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKW-IDVETKD------IVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQ--HKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQI-FTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQP 2306
            E + ++ +   L I+    ++ G Y C VT    +  + +   V V+ PP  I     ++ N     + + ++C V    PP  ++ +        R  I         + E     + +   +E+     C A N AG    N  +  I   P IK P V    +   +    L C V++NP   I W +     I D + +          + K +    G   C A+N  G   ++ Q+ V+  PL   +S Y+      +    ++ C V+  P P+ I W     + +       A L +D   L+F  +     G Y CV Q     + G     + E ++          P +  +  + +  G+   + C  D ++  +   T  W I+    D      I SD     + +++  +NGKY C   N  GK+    T  + V E P  +      N+K+  +       I   C+  G P     W+     +          D   + +E +S +   KLT                  + + +C A+N+ G AS D+F+     P  K       I        + + C   S+    I W K      LP  ++  +IF  + T+                LTI +   D    Y+CVA+N  G    +F+VV +  P
Sbjct: 1315 EKKFSVDNLGTLRIYEADKNDIGNYNCVVTNEAGTSQMTTH--VDVQEPP--IILPSTQTNNTAVVGDRVELKCYVEAS-PPASVTWF--------RRGIAIGTDTKGYVVESDGTLVIQSASVEDATIYTCKASNPAGKAEANLQVTVIA-SPDIKDPDVVTQESIKESHPFSLYCPVFSNPLPQISWYLNDKPLIDDKTSWKTSDDKRKLHVFKAKITDSGVYKCVARNAAGEGSKSFQVEVIV-PLNLDESKYKKKVFAKEGEEVTLGCPVSGFPVPQ-INWVVDGTVVEPGKKYKGATLSNDGLTLHFDSVSVKQEGNYHCVAQ-----SKGNILDIDVELSVLAV-------PIVGEDDNLEVFLGKDISLSC--DLQTESDDKTTFVWSINGSESDRPDNVQIPSDGHRLYITDAKPENNGKYMCRVTNSAGKA--ERTLTLDVLEPPVFVEPVFEANQKLIGN-----NPIILQCQVTGNPKPTVIWKIDGNDV----------DKSWLFDESLSLLRIEKLT----------------GKSAQISCTAENKAGTASRDFFIQNIAAPTFKNEGDQETIFRE--SETITLDCPV-SLGDFQITWMKQG----LPL-TENDAIFTLDNTR----------------LTILNANRDHEDIYTCVANNTAGQVSKDFDVVVQVLP 1916          

HSP 11 Score: 67.3958 bits (163), Expect = 7.887e-11
Identity = 115/559 (20.57%), Postives = 209/559 (37.39%), Query Frame = 3
Query:  702 ENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQWVI----MQGLQIIDSSKFIAKIRP--DMDGPLICEAKNYLGSTKETVQLNVLRDPLIHIDSNYRV--PKNSNYSINCRVTSNPPPEF-IKWYKLS-NMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIIS--IMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETKDIVSDM------ETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTN------EHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQP 2306
            EN G  MC   N AG   +  +L  ++    ++P        + N  I L C+V  NP  T+ W I    +    + D S  + +I         + C A+N  G+      +  +  P    + +      ++   +++C V+     +F I W K    +T++ A+   D+ +L  +  +R    +Y CV  N    T G+       K   + V   PK    + N +++  I +GE+ I+ C  +    G P PT KW D    D+  +        T  V N    H G YKC A N++G++   +T  V V   P   +     ++ +   +L +SI+  C+          W         + + F  E              T     L    F    S ++  +G Y C   N  G+A+  + L ++ PP I        +   +  N +++ C+        + W K        + + +  + +++ I  AE+                              +Y C+A ND G  +++F+V   ++P
Sbjct: 1708 ENNGKYMCRVTNSAGKAERTLTLDVLEPPVFVEPVFEANQKLIGNNPIILQCQVTGNPKPTVIWKIDGNDVDKSWLFDESLSLLRIEKLTGKSAQISCTAENKAGTASRDFFIQNIAAPTFKNEGDQETIFRESETITLDCPVSLG---DFQITWMKQGLPLTENDAIFTLDNTRLTILNANRDHEDIYTCVANN----TAGQ-----VSKDFDVVVQVLPK----IKNAVVTLEINEGEEIILTCDAE----GNPTPTAKW-DFNQGDLPKEAVFVNNNHTVVVNNVTKYHTGVYKCYATNKVGQAV--KTINVHVRTKPRFESGLTESELTV---NLTRSITLECDVDDAIGVGISWTV-------NGKPFLAE--------------TDGVQTLAGGRFLHIVSAKTDDHGSYACTVTNEAGVATKTFNLFVQVPPTIVNEGGEYTV---IENNSLVLPCEVTGKPNPVVTWTKDGRPVGDLKSVQVLSEGQQFKIVHAEIAHK--------------------------GSYICMAKNDVGTAEISFDVDIITRP 2190          

HSP 12 Score: 63.929 bits (154), Expect = 1.081e-9
Identity = 127/631 (20.13%), Postives = 239/631 (37.88%), Query Frame = 3
Query:  525 EQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIK-----YKPKIKPFVPNTVNAM-------INQTID--------LSCEVYANPAATIQWVIMQGLQII----------DSSKFIAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLIH-IDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETKDI---VSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNR-KVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHR-NISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQPD 2309
            E + + + C V  G P P I ++I  D  ++         P   I    ++   R    ++ G   C A++ AG  T + ++  IK     YKP+       T++ M       +N T D        + C V   P   I W  + G  I           D++  + K      G   C+A N  G  ++   + ++  P+I    S++ +  +  ++I C V  +P P  I W  L +    +  ++++D  L    ++ +  G + C  QN            N  +T+ LTV   P        KI   ++ +  +++C      P     T +   ++++ I   + +     + N +  + G + C   N  G+     T  +TV+E P II++      V   ++     I   C   G P     W         +  D K+++ + ++ N    +I+     D+                G YNC   N  G + +   + ++ PPII  + +    A   ++  V ++C  E+     + W++    I +   +K Y + E++ T                 L I+    +D + Y+C ASN  G  + N +V   + PD
Sbjct:  900 EGQDLTLSCVVVLGTPKPSI-VWIKDDKPVEE-------GPTIKIEGGGSLLRLRGGNPKDEGKYTCIAVSPAGNSTLHINVQLIKKPEFVYKPEGGIVFKPTISGMDEKHVAVVNSTHDVLDGEGFAIPCVVSGTPPPIITWY-LDGRPITPNSRDFTVTADNTLIVRKADKSYSGVYTCQATNSAGDNEQKTTIRIMNTPMISPGQSSFNMVVDDLFTIPCDVYGDPKP-VITW--LLDDKPFTEGVVNEDGSLTIPNVNEAHRGTFTCHAQNAA---------GNDTRTVTLTVHTTPTINAENQEKIA--LQNDDIVLECPAKALPPPVRLWTYEGEKIDSQLIPHTIREDGALVLQNVKLENTGVFVCQVSNLAGEDSLSYT--LTVHEKPKIISEVPGVVDVVKGFT-----IEIPCRATGVPEVIRTWNK-------NGIDLKMDEKKFSVDNLGTLRIYEADKNDI----------------GNYNCVVTNEAGTSQMTTHVDVQEPPIILPSTQTNNTAVVGDR--VELKCYVEASPPASVTWFRRG--IAIGTDTKGY-VVESDGT-----------------LVIQSASVEDATIYTCKASNPAGKAEANLQVTVIASPD 1455          

HSP 13 Score: 61.6178 bits (148), Expect = 5.083e-9
Identity = 132/640 (20.62%), Postives = 233/640 (36.41%), Query Frame = 3
Query:  153 ITPNNATVNSKENLTLECVVDQQ-NIVYSWEY--KPFHIKSNIMSKVLGFCYFEKPCVVPDSESQITISHESRLTIHNISSDNSGYYVCRVTLLTTSEHIISLAV--VTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSL-LNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQWVIMQGLQIIDSSKFIAKI--------------RPDMDGPLICEAKNYLGSTKETVQLNVLRDPLIHI---DSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMTDSSAVLISDDA-----------KLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSP--------GEPFPTLKWIDVETKDIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFL 1946
            +T +  TVN   ++TLEC VD    +  SW    KPF  +++ +  + G  +                     L I +  +D+ G Y C V    T+E  ++     + V+ PP  ++     ++    E   + + C V+G   P  +  +      +  LK +        ++E     +     + ++G  +C A ND G  T   S  ++I  +P I+  + N V A+    +   C +  +     Q + ++  Q ID     A+I                + +G   C  KN  G      +  VL  P I +   D N    ++S  +++C  T  P P+ I W+K     D  A+ I + A           +L    I     G Y C   N            + E+ + + V   PK  +          + E+ +I C    + P        G+P  + K+  V+T      +  F++  ++S    KY C+A NE G    +    V++  APS    +  R++ ++  + P ++   C  +G P     W      I P+ +    +  R +                      Q +    S  GRY C A N  G   L+  L
Sbjct: 2011 LTESELTVNLTRSITLECDVDDAIGVGISWTVNGKPFLAETDGVQTLAGGRF---------------------LHIVSAKTDDHGSYACTV----TNEAGVATKTFNLFVQVPPTIVNEGGEYTV---IENNSLVLPCEVTG--KPNPVVTWTKDGRPVGDLKSV------QVLSEGQQFKIVHAE-IAHKGSYICMAKNDVG--TAEISFDVDIITRPMIQKGIKNIVTAIKGGALPFKCPIDDDKNFKGQIIWLRNYQPIDLEAEDARITRLSNDRRLTILNVTENDEGQYSCRVKNDAGENSFDFKATVLVPPTIIMLDKDKNKTAVEHSTVTLSCPATGKPEPD-ITWFK-----DGEAIHIENIADIIPNGELNGNQLKITRIKEGDAGKYTCEADN---------SAGSVEQDVNVNVITIPKIEKDGIPSDYESQQNERVVISCPVYARPPAKITWLKAGKPLQSDKF--VKTSANGQKLYLFKLRETDSS---KYTCIATNEAGT--DKRDFKVSMLVAPSFDEPNIVRRITVNSGN-PSTLH--CPAKGSPSPTITWLKDGNAIEPNDRYVFFDAGRQL----------------------QISKTEGSDQGRYTCIATNSVGSDDLENTL 2564          

HSP 14 Score: 55.4546 bits (132), Expect = 3.682e-7
Identity = 45/196 (22.96%), Postives = 78/196 (39.80%), Query Frame = 3
Query:  705 NRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQWVIMQ-------GLQIIDSSKF--IAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLIHIDSN-YRVPKNSNYSINCRVTSNPPPEFIKWYK-LSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNI 1259
            + G   C A N  G+ T+   L +I   P +K   P  V   + + ++LSCE   +P   + W             Q+   SKF  I       +G   C A +  G  ++T  L +   P + I  N   V +    S  C+     P   I+W+K   ++      +  ++ +L+ +       G Y+CV +N+
Sbjct:  584 DAGVYECMARNAGGMSTRKMRL-DIMEPPSVK-VTPQDVYFNMREGVNLSCEAMGDPKPEVHWYFKGRHLLNDYKYQVGQDSKFLYIRDATHHDEGTYECRAMSQAGQARDTTDLMLATPPKVEIIQNKMMVGRGDRVSFECKTIRGKPHPKIRWFKNGKDLIKPDDYIKINEGQLHIMGAKDEDAGAYSCVGENM 777          

HSP 15 Score: 54.6842 bits (130), Expect = 6.361e-7
Identity = 96/423 (22.70%), Postives = 164/423 (38.77%), Query Frame = 3
Query:  516 VFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMIN--QTIDLSCEVYANPAATIQWVIMQGL--------QIIDSSKFIAKIRPDMDGPLICEAKNYLGSTKETVQLNV----LRDPLIHIDSNYRV-PKNSNYSINCRVTSNPPPEFIKWYKLSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQ-ILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWID----VETKDIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNE----APSIITQHKNRKVFLSYS-DLPQSISFTCEFRGQPL 1709
            V  + + + + C  SG +P P I+ Y  KDN     +++++   + T        ++        G   C A N      ++  L+ +   P++   VP  +N   N  QT+ LSC     P   I W+    +        QI+ ++  I  + PD DG   C AK+  G    T +L V     R   I ++ + +  PK + Y I+   T +  P+ + W  +            +D+ LN  +        Y C+         G R    S +T+ L        PQ I+  K  +   G    ++CS    + G P PT+ W      +E           +V  + +  +G+Y C+A+N +G S    +A + V+      P   +  KN  V   Y  +   S   T E+ G P+
Sbjct: 4328 VVHKGDQVTLWCEASG-VPQPAITWY--KDN-----ELLTNTGVDETATTKKKSVIFSSISPSQAGVYTCKAENWVASTEEDIDLI-VMIPPEV---VPERMNVSTNPRQTVFLSCNATGIPEPVISWMRDSNIAIQNNEKYQILGTTLAIRNVLPDDDGFYHCIAKSDAGQKIATRKLIVNKPSDRPAPIWVECDEKGKPKKTEYMIDRGDTPDDNPQLLPWKDV------------EDSSLNGSI-------AYRCM--------PGPR----SSRTVLLHAA-----PQFIVKPKNTTAAIGAIVELRCS----AAGPPHPTITWAKDGKLIEDSKFEIAYSHLKVTLNSTSDSGEYTCMAQNSVGSSTV--SAFINVDNNILPTPKPSSNQKNVAVITCYERNQAYSRGLTWEYNGVPM 4696          
BLAST of hemicentin-1 isoform X2 vs. Ensembl Celegans
Match: syg-2 (Synaptogenesis protein syg-2 [Source:UniProtKB/Swiss-Prot;Acc:Q9U3P2])

HSP 1 Score: 75.485 bits (184), Expect = 1.676e-13
Identity = 56/222 (25.23%), Postives = 107/222 (48.20%), Query Frame = 3
Query: 1884 NGRYNCEADNRHGI-ASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSN-YSCVASNDQGIKKVNFEVVFKSQPDPPYDLRVTDFEWDSVTINWKPGFNGGLTQNYEILVISENFGTQNVPFYSDKNENTLTITGLYPSTPYEIRIYAVNSIG 2543
            +G Y C+ADN  G+ A    FL++   P +    R+ K A  L   V   RC+  ++ T+   W K  +  ++   S KYSI   ++     E  L+          IK++  DD++    C+A N  G   ++  ++  + PD P++L +T+   +++++ W+P F+GG  Q +E+    +N    ++    +     L ++GL  +  Y  +I ++N+ G
Sbjct:  295 SGEYTCKADNGIGVPAEGQTFLLVNSAPRVLPVARYAKTAGILG-GVAKARCKVYAVPTVEFVWEKDEQ--LIKKNSSKYSIVNTQIDYSTYESTLW----------IKNIVPDDYTKKVKCIARNSFGTDHLSIPIIPPTHPDEPFNLTMTNSTLNTISVAWEPRFDGGSDQIFEVKYRRQN---DDLIHLVNTTHTNLRLSGLATANTYFFQIRSINARG 500          
BLAST of hemicentin-1 isoform X2 vs. Ensembl Fly
Match: ed (gene:FBgn0000547 transcript:FBtr0334265)

HSP 1 Score: 155.606 bits (392), Expect = 7.804e-38
Identity = 164/695 (23.60%), Postives = 296/695 (42.59%), Query Frame = 3
Query:  363 LTIHNISSD-NSGYYVCRVTLLTTSEHI-ISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNS-TIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANP-AATIQWVIMQGLQIIDSS--KFIAKIRPDMDGPLICEAKNYLGSTKET-VQLNVLRDPLIHIDSNYR-VPKNSNYSINCRVTSNPPPEFIKWYKLSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKW---IDVE---TKDIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIIT---QHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQPDPPYDLRVTDFEWDSVTINWKPGFNGGLTQ 2396
            L I N+S + ++G + CR+    T   +      +TV TPP         +I V  E + + + CS  GG P P I+ Y    N      ++     +  T A +S I        + +  V   A+N+   L + T+ LN+ Y P+++    N +    ++T  L C V A P    ++W   +  + I SS    I ++     G   C A N LG T E  + L++L  P++ I+S  R   +    +I C VT+NP P  I+W K     ++S     +   L    +     G Y C   NI  S   +R       T+ L V ++P +  I  NK + +  G    + CS +   PG P P  +W   +D E   T+ I++    + +  +   + GKY C A NE+G      T ++ +++ P  +    QH  R+V  +        + TC  +G+P    +W     +I P    ++++ + +     +  + ++L     +R      +L     G Y C   N   + S +  + ++         ++ K+A  + +   ++ C+ ++      +W   N    L   S  +     E++      D++T  LK+N LT     + D+  Y+C  +N     +    +  K  P+ P +LR T+   + V+++W PGF+GGL++
Sbjct:  110 LQIKNVSYNRDNGRFECRIKAKGTGADVHQEFHNLTVLTPPHPPVISPG-NIAVATEDKPMELTCSSIGGSPDPTITWYREGSNTPLPATVLKGGTKDQPTNATLSIIPRREDDGAKYKCVVRNRAMNEGKRL-EATATLNVNYYPRVEVGPENPLRVERDRTAKLECNVDAKPKVPNVRW--NRNGRFISSSLVHTIHRVSVQDAGKYTCIADNGLGKTGEQELILDILYPPMVVIESKTREAEEGDTVTIRCNVTANPAPVTIEWLK-----ENSPDFRYNGDVLTLTSVRADHAGNYICRAVNIMQSQGMERSERVGNSTVALLVRHRPGQAYITPNKPV-VHVGNGVTLTCSAN--PPGWPVPQYRWFRDMDGEFSSTQKILAQGPQYSIPKAHLGNEGKYHCHAVNELGIGM-MATIVLEIHQPPQFLAKLQQHMTRRVADT------DYTVTCSAKGKPAPSVKWLKDAVEILPEENLYEVQTNPDQGLNGMVTVQSQLKFRGKAR--PNGNALVPGDRGLYTCLYQNE--VNSANSSMQLRIEHEPIVLHQYNKVAFDIRETAEVV-CKVQAYPKPEFQWQFGNNPSPLTMSSDGHY----EISTTTDNNDIYTSVLKINSLT-----HSDYGEYTCRVANTLDTIRAPIRLQQKGPPEKPTNLRATEVGHNYVSLSWDPGFDGGLSK 771          
BLAST of hemicentin-1 isoform X2 vs. Ensembl Fly
Match: ed (gene:FBgn0000547 transcript:FBtr0077465)

HSP 1 Score: 155.606 bits (392), Expect = 7.804e-38
Identity = 164/695 (23.60%), Postives = 296/695 (42.59%), Query Frame = 3
Query:  363 LTIHNISSD-NSGYYVCRVTLLTTSEHI-ISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNS-TIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANP-AATIQWVIMQGLQIIDSS--KFIAKIRPDMDGPLICEAKNYLGSTKET-VQLNVLRDPLIHIDSNYR-VPKNSNYSINCRVTSNPPPEFIKWYKLSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKW---IDVE---TKDIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIIT---QHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQPDPPYDLRVTDFEWDSVTINWKPGFNGGLTQ 2396
            L I N+S + ++G + CR+    T   +      +TV TPP         +I V  E + + + CS  GG P P I+ Y    N      ++     +  T A +S I        + +  V   A+N+   L + T+ LN+ Y P+++    N +    ++T  L C V A P    ++W   +  + I SS    I ++     G   C A N LG T E  + L++L  P++ I+S  R   +    +I C VT+NP P  I+W K     ++S     +   L    +     G Y C   NI  S   +R       T+ L V ++P +  I  NK + +  G    + CS +   PG P P  +W   +D E   T+ I++    + +  +   + GKY C A NE+G      T ++ +++ P  +    QH  R+V  +        + TC  +G+P    +W     +I P    ++++ + +     +  + ++L     +R      +L     G Y C   N   + S +  + ++         ++ K+A  + +   ++ C+ ++      +W   N    L   S  +     E++      D++T  LK+N LT     + D+  Y+C  +N     +    +  K  P+ P +LR T+   + V+++W PGF+GGL++
Sbjct:  110 LQIKNVSYNRDNGRFECRIKAKGTGADVHQEFHNLTVLTPPHPPVISPG-NIAVATEDKPMELTCSSIGGSPDPTITWYREGSNTPLPATVLKGGTKDQPTNATLSIIPRREDDGAKYKCVVRNRAMNEGKRL-EATATLNVNYYPRVEVGPENPLRVERDRTAKLECNVDAKPKVPNVRW--NRNGRFISSSLVHTIHRVSVQDAGKYTCIADNGLGKTGEQELILDILYPPMVVIESKTREAEEGDTVTIRCNVTANPAPVTIEWLK-----ENSPDFRYNGDVLTLTSVRADHAGNYICRAVNIMQSQGMERSERVGNSTVALLVRHRPGQAYITPNKPV-VHVGNGVTLTCSAN--PPGWPVPQYRWFRDMDGEFSSTQKILAQGPQYSIPKAHLGNEGKYHCHAVNELGIGM-MATIVLEIHQPPQFLAKLQQHMTRRVADT------DYTVTCSAKGKPAPSVKWLKDAVEILPEENLYEVQTNPDQGLNGMVTVQSQLKFRGKAR--PNGNALVPGDRGLYTCLYQNE--VNSANSSMQLRIEHEPIVLHQYNKVAFDIRETAEVV-CKVQAYPKPEFQWQFGNNPSPLTMSSDGHY----EISTTTDNNDIYTSVLKINSLT-----HSDYGEYTCRVANTLDTIRAPIRLQQKGPPEKPTNLRATEVGHNYVSLSWDPGFDGGLSK 771          
BLAST of hemicentin-1 isoform X2 vs. Ensembl Fly
Match: ed (gene:FBgn0000547 transcript:FBtr0334266)

HSP 1 Score: 155.606 bits (392), Expect = 8.493e-38
Identity = 164/695 (23.60%), Postives = 296/695 (42.59%), Query Frame = 3
Query:  363 LTIHNISSD-NSGYYVCRVTLLTTSEHI-ISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNS-TIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANP-AATIQWVIMQGLQIIDSS--KFIAKIRPDMDGPLICEAKNYLGSTKET-VQLNVLRDPLIHIDSNYR-VPKNSNYSINCRVTSNPPPEFIKWYKLSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKW---IDVE---TKDIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIIT---QHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQPDPPYDLRVTDFEWDSVTINWKPGFNGGLTQ 2396
            L I N+S + ++G + CR+    T   +      +TV TPP         +I V  E + + + CS  GG P P I+ Y    N      ++     +  T A +S I        + +  V   A+N+   L + T+ LN+ Y P+++    N +    ++T  L C V A P    ++W   +  + I SS    I ++     G   C A N LG T E  + L++L  P++ I+S  R   +    +I C VT+NP P  I+W K     ++S     +   L    +     G Y C   NI  S   +R       T+ L V ++P +  I  NK + +  G    + CS +   PG P P  +W   +D E   T+ I++    + +  +   + GKY C A NE+G      T ++ +++ P  +    QH  R+V  +        + TC  +G+P    +W     +I P    ++++ + +     +  + ++L     +R      +L     G Y C   N   + S +  + ++         ++ K+A  + +   ++ C+ ++      +W   N    L   S  +     E++      D++T  LK+N LT     + D+  Y+C  +N     +    +  K  P+ P +LR T+   + V+++W PGF+GGL++
Sbjct:  110 LQIKNVSYNRDNGRFECRIKAKGTGADVHQEFHNLTVLTPPHPPVISPG-NIAVATEDKPMELTCSSIGGSPDPTITWYREGSNTPLPATVLKGGTKDQPTNATLSIIPRREDDGAKYKCVVRNRAMNEGKRL-EATATLNVNYYPRVEVGPENPLRVERDRTAKLECNVDAKPKVPNVRW--NRNGRFISSSLVHTIHRVSVQDAGKYTCIADNGLGKTGEQELILDILYPPMVVIESKTREAEEGDTVTIRCNVTANPAPVTIEWLK-----ENSPDFRYNGDVLTLTSVRADHAGNYICRAVNIMQSQGMERSERVGNSTVALLVRHRPGQAYITPNKPV-VHVGNGVTLTCSAN--PPGWPVPQYRWFRDMDGEFSSTQKILAQGPQYSIPKAHLGNEGKYHCHAVNELGIGM-MATIVLEIHQPPQFLAKLQQHMTRRVADT------DYTVTCSAKGKPAPSVKWLKDAVEILPEENLYEVQTNPDQGLNGMVTVQSQLKFRGKAR--PNGNALVPGDRGLYTCLYQNE--VNSANSSMQLRIEHEPIVLHQYNKVAFDIRETAEVV-CKVQAYPKPEFQWQFGNNPSPLTMSSDGHY----EISTTTDNNDIYTSVLKINSLT-----HSDYGEYTCRVANTLDTIRAPIRLQQKGPPEKPTNLRATEVGHNYVSLSWDPGFDGGLSK 771          
BLAST of hemicentin-1 isoform X2 vs. Ensembl Fly
Match: fred (gene:FBgn0051774 transcript:FBtr0308126)

HSP 1 Score: 151.754 bits (382), Expect = 1.026e-36
Identity = 183/791 (23.14%), Postives = 318/791 (40.20%), Query Frame = 3
Query:  363 LTIHNISSD-NSGYYVCRVTLLTTS----EHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKR---LKIISSN-FPNSTIAEVSAISLWRPHYLENRGFVMCTAINDA---GVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAAT-IQWVIMQGLQIIDSSKFIAKIRPDMDGPLICEAKNYLGSTKET-VQLNVLRDPLIHIDS-NYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWI-DVE-----TKDIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIIT---QHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQPDPPYDLRVTDFEWDSVTINWKPGFNGGLTQNYEIL-----------VISENFGTQNVPFYSDK----------------NENTLTITGLYPSTPYEIRIYAVNSIGRSLNSETVKCVTK 2582
            L I N S + ++G + CR+    T     +   +L V+T   PP         ++ V  E++ + + CS  GG P P I+ Y        +   LK  S N + N+T+  V       P   ++     C   N A   G + + +  LN+ Y P+++    N +    +    L C V A P  + ++W           +  I ++     G   C A N LG T E  + L+VL  P++ I+S  +   +     I C VT+NP P  ++W K     + +         L    +     G Y C + NI    + KR       T+ L V ++P +  I  NK + +  G    + CS +   PG P P  +W  D++     T+ I++    + +  +     GKY C A NE+G      T ++ V++ P  +    QH  R+V     D+  ++  TC  +G+P  + RW     +I P+ + F I      +   +  + + L     +R    Q  L  +  G Y C  +N    A+    L I++ PI+ +  ++ K+A  L ++  ++ C+ ++      +W   N    L   S  +     E++      D++T  L+     I  L++ D+  Y C A N     +    +  K  P+ P +L++ +   +   +NW PGFNGG      ++            +S+  G   +P Y                    EN   +  L     Y  ++YA+NS G S  S  +   TK
Sbjct:   89 LQIKNTSYNRDNGRFECRIKAKGTGADVHQEFYNLTVLTAPHPPMVTP----GNLAVATEEKPLELTCSSIGGSPDPMITWYREGSTVPLQSYALKGGSKNHYTNATLQIV-------PRRADDGAKYKCVVWNRAMPEGHMLETSVTLNVNYYPRVEVGPQNPLKVERDHVAKLDCRVDAKPMVSNVRWSRNGQYVSATPTHTIYRVNRHHAGKYTCSADNGLGKTGEKDIVLDVLYPPIVFIESKTHEAEEGETVLIRCNVTANPSPINVEWLK-----EGAPDFRYTGELLTLGSVRAEHAGNYICRSVNIMQPFSSKRVEGVGNSTVALLVRHRPGQAYITPNKPV-VHVGNGVTLTCSAN--PPGWPVPQYRWFRDMDGDIGNTQKILAQGPQYSIPKAHLGSEGKYHCHAVNELGIG-KIATIILEVHQPPQFLAKLQQHMTRRV----GDVDYAV--TCSAKGKPTPQIRWIKDGTEILPTRKMFDIRTTPTDAGGGVVAVQSILRFRGKARPNGNQ--LLPNDRGLYTCLYENDVNSANSSMHLRIEHEPIVIH--QYNKVAYDLRESAEVV-CRVQAYPKPEFQWQYGNNPSPLTMSSDGHY----EISTRMENNDVYTSILR-----IAHLQHSDYGEYICRAVNPLDSIRAPIRLQPKGSPEKPTNLKILEVGHNYAVLNWTPGFNGGFMSTKYLVSYRRVATPREQTLSDCSGNGYIPSYQISSSSSNSNHEWIEFNCFKENPCKLAPLDQHQSYMFKVYALNSKGTSGYSNEILATTK 839          
BLAST of hemicentin-1 isoform X2 vs. Ensembl Fly
Match: fred (gene:FBgn0051774 transcript:FBtr0308125)

HSP 1 Score: 150.984 bits (380), Expect = 2.295e-36
Identity = 183/791 (23.14%), Postives = 318/791 (40.20%), Query Frame = 3
Query:  363 LTIHNISSD-NSGYYVCRVTLLTTS----EHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKR---LKIISSN-FPNSTIAEVSAISLWRPHYLENRGFVMCTAINDA---GVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAAT-IQWVIMQGLQIIDSSKFIAKIRPDMDGPLICEAKNYLGSTKET-VQLNVLRDPLIHIDS-NYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWI-DVE-----TKDIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIIT---QHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQPDPPYDLRVTDFEWDSVTINWKPGFNGGLTQNYEIL-----------VISENFGTQNVPFYSDK----------------NENTLTITGLYPSTPYEIRIYAVNSIGRSLNSETVKCVTK 2582
            L I N S + ++G + CR+    T     +   +L V+T   PP         ++ V  E++ + + CS  GG P P I+ Y        +   LK  S N + N+T+  V       P   ++     C   N A   G + + +  LN+ Y P+++    N +    +    L C V A P  + ++W           +  I ++     G   C A N LG T E  + L+VL  P++ I+S  +   +     I C VT+NP P  ++W K     + +         L    +     G Y C + NI    + KR       T+ L V ++P +  I  NK + +  G    + CS +   PG P P  +W  D++     T+ I++    + +  +     GKY C A NE+G      T ++ V++ P  +    QH  R+V     D+  ++  TC  +G+P  + RW     +I P+ + F I      +   +  + + L     +R    Q  L  +  G Y C  +N    A+    L I++ PI+ +  ++ K+A  L ++  ++ C+ ++      +W   N    L   S  +     E++      D++T  L+     I  L++ D+  Y C A N     +    +  K  P+ P +L++ +   +   +NW PGFNGG      ++            +S+  G   +P Y                    EN   +  L     Y  ++YA+NS G S  S  +   TK
Sbjct:   89 LQIKNTSYNRDNGRFECRIKAKGTGADVHQEFYNLTVLTAPHPPMVTP----GNLAVATEEKPLELTCSSIGGSPDPMITWYREGSTVPLQSYALKGGSKNHYTNATLQIV-------PRRADDGAKYKCVVWNRAMPEGHMLETSVTLNVNYYPRVEVGPQNPLKVERDHVAKLDCRVDAKPMVSNVRWSRNGQYVSATPTHTIYRVNRHHAGKYTCSADNGLGKTGEKDIVLDVLYPPIVFIESKTHEAEEGETVLIRCNVTANPSPINVEWLK-----EGAPDFRYTGELLTLGSVRAEHAGNYICRSVNIMQPFSSKRVEGVGNSTVALLVRHRPGQAYITPNKPV-VHVGNGVTLTCSAN--PPGWPVPQYRWFRDMDGDIGNTQKILAQGPQYSIPKAHLGSEGKYHCHAVNELGIG-KIATIILEVHQPPQFLAKLQQHMTRRV----GDVDYAV--TCSAKGKPTPQIRWIKDGTEILPTRKMFDIRTTPTDAGGGVVAVQSILRFRGKARPNGNQ--LLPNDRGLYTCLYENDVNSANSSMHLRIEHEPIVIH--QYNKVAYDLRESAEVV-CRVQAYPKPEFQWQYGNNPSPLTMSSDGHY----EISTRMENNDVYTSILR-----IAHLQHSDYGEYICRAVNPLDSIRAPIRLQPKGSPEKPTNLKILEVGHNYAVLNWTPGFNGGFMSTKYLVSYRRVATPREQTLSDCSGNGYIPSYQISSSSSNSNHEWIEFNCFKENPCKLAPLDQHQSYMFKVYALNSKGTSGYSNEILATTK 839          
BLAST of hemicentin-1 isoform X2 vs. Ensembl Zebrafish
Match: hmcn1 (hemicentin 1 [Source:ZFIN;Acc:ZDB-GENE-041014-322])

HSP 1 Score: 110.153 bits (274), Expect = 1.471e-23
Identity = 157/688 (22.82%), Postives = 283/688 (41.13%), Query Frame = 3
Query:  327 SESQITISHESRLT-IHNISSDNSGYYVCRVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFI-----AIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSN-----FPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPF---VPNTVNAMINQTIDLSCEVYANPAATIQWVI-MQGLQIIDSSKFIAKIRPDM--------DGPLICEAKNYLGSTKETVQLNVLRDPLIHI--DSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMTDSS--AVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPAN-SEKTMFLTVTYKPKEPQILGN--KIISIMKGEKFIIKCSTDRKSPGEPFPTLKWID-----VETKD--IVSDMETFQVINSESRHNGKYKCLAENEIG---KSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRS--SPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQG 2264
            ++  +TI+   R+  I +    ++G Y C  T +   +       V ++ PP    F  +   N  + ++ I     ++ C  +  +PPP ++ Y       K   +++SN      P   + ++    +      ++ G   C A+N+AG  +    +  +   P I+     +P+ V  ++N+T+ L C+V  +P   I WV   Q L   ++ + ++  R            G  +C A+N  G+ +++  L+V   P I    + N  V  N+  S+NC  T  PPP  ++W+K      +S  A+++     L  +    S  G Y+CV  N          PA  ++K ++LTV   P      G+   ++++  G+  +++C ++   P    P + W        ET +  I++D +  Q+  +E    G+Y C A N  G   K+F      + ++  P +    + R V      +   ++F C+  G              IPP S  +       + N R+ +    L + + S     Q S RS  S +G Y C A N  G A  +Y L+I  PP I  +    +++  LN +V ++ C+AE   T  I+W K    I    Q            K  P+          + LT+  +   D   Y+CVA+N  G
Sbjct: 2706 ADGHLTITANGRIVQIKHTQVSDTGRYTCVATNIAGEDE--KDFDVNIQVPPSFRGFGDSGPGNNGEVKDVILNNPISLYCETNA-VPPPVLTWY-------KDGHLLTSNDKVLILPGGRVLQIPRAQV------DDTGRYTCVAVNEAGEDSIQYDV-RVLLPPSIRGADGELPDEVTVLVNKTMLLECQVDGSPTPKISWVKDSQPLTQDNTHRLLSNGRTLQILNAQVTDTGRYVCVAQNLAGTAEKSFNLHVHVSPTIVGIREENVTVVLNNFVSLNCEATGLPPPT-LRWFKDRRPVQASTNALIMPGGRTLQILKAKMSDGGKYSCVAVN----------PAGEAQKLIYLTVYVPPSIRHNSGDLPVVLNVQVGKSVMLECESNAVPP----PVITWYKNGRPIAETANLRILADGQMLQLKETEVSDTGQYVCKATNVAGQVDKNFH-----LNIHVPPRLDGPAEERVV----ETISNPVTFACDATG--------------IPPPSLTW-------LKNGRVIENSESLEMHILSGGSKLQIS-RSQLSDSGTYTCVASNVEGKAQKNYHLVIHVPPNIIGSELPSEMSVLLNDSVQLV-CRAEGTPTPEIQWLKDGMTISRTAQKN---------IKISPDG---------STLTVTAVHTSDSGKYTCVATNQAG 3311          

HSP 2 Score: 100.138 bits (248), Expect = 1.491e-20
Identity = 152/680 (22.35%), Postives = 251/680 (36.91%), Query Frame = 3
Query:  363 LTIHNISSDNSGYYVCRVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKII--SSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKI-KPFVPNTVNAMINQTIDLSCEVYANPAATIQWVIMQGLQIIDSSKFIAKIRPDMD------------GPLICEAKNYLGSTKETVQLNVLRDPLIHIDSNYRVPK------NSNYSINCRVTSNPPPEFIKWYK--LSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVE---TKD----IVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPG--LKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQP 2306
            L +      ++G YVC+ T    +  +     + +  PP+       + +        +   C  +G IPPP ++        +K  ++I  S +     ++  S + + R   L + G   C A N  G   KN  L+ I   P I    +P+ ++ ++N ++ L C     P   IQW +  G+ I  +++   KI PD              G   C A N  G       LNV   PLI    N    K      +S+ +I C  + +PPP+ + W K  L     S   L+S    L       S  G Y CV  N         +     +   L V   P        + ++++KG      C  D    G P P + W+      +KD    +++   T Q++N    H G+Y C A N+ G + SR  +L  V + P I       +V +  + +   +   CE  G PL    W      +P +          NI   R  Q+          R  S Q        GRY C A +  G    + FL+  + P      R  +  + L    V + C+++++    + W K +  +                 K  P   + + G  L++N   +      D + YSCVASN  G  + +F++     P
Sbjct: 3100 LQLKETEVSDTGQYVCKAT--NVAGQVDKNFHLNIHVPPRLDGPAEERVVETISNP--VTFACDATG-IPPPSLTW-------LKNGRVIENSESLEMHILSGGSKLQISRSQ-LSDSGTYTCVASNVEGKAQKNYHLV-IHVPPNIIGSELPSEMSVLLNDSVQLVCRAEGTPTPEIQW-LKDGMTISRTAQKNIKISPDGSTLTVTAVHTSDSGKYTCVATNQAGEEDRIFNLNVYVPPLIQ--GNGPAAKELTTVLDSSINIECVASGSPPPQ-LNWLKNGLPLPVSSQIRLLSAGQVLRIARAQVSDGGSYTCVASN---------RAGVDNRHYTLQVYVPPSLDGAGSTEEVTVVKGSTASFICIAD----GTPSPVIIWLRTGASVSKDAHISLLNQNSTMQIVNVLVNHTGRYTCTAHNQAGDA-SRHFSL-KVLDPPRINGSGVPAEVSVVVNHV---LELLCEADGIPLPTLTWLKDGRPLPQT---------ENIRLLRDGQML---------RVASAQV----ENTGRYTCLASSPAGDDDKE-FLVRVHVPPNIAGDRGVQDVSVLQNRQVTLECKSDAVPPPTLTWLKDDAPL-----------------KMSPRVRVLSSGRYLQINNAVL-----GDGAQYSCVASNVAGETRRHFKLSVNVPP 3698          

HSP 3 Score: 93.5893 bits (231), Expect = 1.460e-18
Identity = 152/708 (21.47%), Postives = 265/708 (37.43%), Query Frame = 3
Query:  318 VPDSESQITISHESRLTIHNISSDNSGYYVCRVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIK---RLKIISSN----FPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQWVIMQGLQII----------DSSKFIAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLI-HIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYK----LSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETKD---IVSDMETFQVINS--------ESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQPDPPYDLRVTDFE 2342
            +P +E+   +     L + +   +N+G Y C  +     +    L  V V  PP        + ++V Q ++ + ++C  S  +PPP ++ ++  D  +K   R++++SS       N+ + + +  S              C A N AG  T+    L++   P IK   P +V+  INQ + L C V   PA  I W   QG  +           D S  I   +    G  +C A N  G+ ++ V L V   P I    +N  V  N   +++C  T  P P  + W K    L+   + +   +     L  I      T +Y CV  N         +     + + LTV   P         ++S +     +I C+      G P P L W    +KD   +  D E F ++ S        +  H+G+Y C+A+N  G +  R   L TV+E+P I +      V L+       ++  C   G P     W+     I  +   F +  +  +                      Q +  +   +G Y C A N  G A     L ++ PP+I  +IR   +A  ++   V ++C +E   T  + W+K  + +    + +  S    ++   +P                      D   Y+C  +N  G   ++  +  +  P     +RV + E
Sbjct: 3554 LPQTENIRLLRDGQMLRVASAQVENTGRYTCLASSPAGDDDKEFL--VRVHVPPNIAGDRGVQDVSVLQNRQ-VTLECK-SDAVPPPTLT-WLKDDAPLKMSPRVRVLSSGRYLQINNAVLGDGAQYS--------------CVASNVAGE-TRRHFKLSVNVPPTIKDG-PQSVSVHINQPVVLECVVNGVPAPRITWR-KQGSILAGNNPRYKFGEDGSLRILSAQVTDTGRYLCMATNQAGTERKRVDLQVYVPPSIAEGPTNITVTVNVQTTLSCEATGIPKPS-VSWTKNTQALNTDQNQNMYRLLSSGSLVVIAPSVEDTALYECVVSN---------EAGQESRAIQLTVHVPPSIADEATELVVSRLS--PVVIGCTAS----GVPHPVLYW----SKDGLRLAKDGEGFTILPSGPLEITAADLSHSGRYSCVAKNAAGTAH-RHVQL-TVHESPVIQSHPSTLDVILN-----NHVTLPCRATGSPRPTITWQKEGINIFTTGGGFTVLPNGGL----------------------QISKAKVEDSGTYMCVAQNPAGTALGKTKLRVQVPPVITSDIRAYTVALDVS---VTLQCNSEGFPTPSVSWHKDGQLLSESVRQRVLSTGALQIAFAQP---------------------GDTGRYTCTVANVAGSSSLDISLTVQIPP----SIRVGESE 4162          

HSP 4 Score: 88.1965 bits (217), Expect = 6.747e-17
Identity = 151/635 (23.78%), Postives = 247/635 (38.90%), Query Frame = 3
Query:  327 SESQITISHESRLTIHNISSDNSGYYVCRVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQ---------EFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQW-----VIMQG--LQIIDSSKFIAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLIHI-DSNYRVP-----KNSNYSINCRVTSNPPPEFIKWYKLSN--MTDSSAVLISDDAKLNFILIDRS--STGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKP--KEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWID-----VETKD--IVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETAL-VTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNG---RYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSK 2114
            S S  +++ +  L IH+ S + SG +VC  T    + +      +TV   PK +S      I+   E+         + I + C V   +PPP I+    KD      K + S F    I   S         + + G  +C A N AG  ++ T  L++   P I    P  +   I   IDL C     P  ++ W     V+  G   +I D +  + ++    +G  +C A N  G  +  +QL+V   P++ + +  +  P      N   +  C     P P  IKW++  +    +   V I +D  L  IL   S    G Y C+  N             +EK   L V   P  ++  +L N  + I +    +   +      G P P + W       V + D  I+   +T +++ +  +  G+Y C A N  G   SRE    + V   P+II    +R +    + L Q I   C+ +G P    +W             +K         +R         I++ +R   QQ  ++S+  G   RY C A N  G  S D+ L I  PP IK      ++   L  N+V + C+   +    + WYK  E I+   Q++
Sbjct: 1016 SGSVFSLTEDGSLHIHSPSGEESGEFVCTAT--NAAGYSSRKVQLTVYVRPK-LSGVGEADIHDNSEKLLEMSVIVGDDITLPCEVES-VPPPIITW--AKD------KQLISPFSPRHIQLPSGSMRITDTRVTDSGMYLCVATNIAGNFSQ-TVKLSVLVPPSISAG-PRAMKVQIGHAIDLPCVTQGVPEPSVSWLKDGTVLQDGSRYRISDGALTLNQVALTDEGIYVCRAVNIAGKEETAIQLHVQVPPVVEVSEPPFNSPLQERVANQQIAFPCPAKGTPEP-VIKWFRNGHELTGNEPGVSILEDGTL-LILASVSPLDNGEYTCMAVN---------DAGTTEKKYQLKVNVPPDIRDNGLLSNVSVVINQPTNLVCDVT------GTPVPVITWYKDGVEVVPSSDVQILQKGKTLKLLKAAVQDAGRYICKAINIAG---SRERDFYLDVLVPPTIIGTVGSRDL---SAVLNQEIVLECKVKGDPFPTIQW-------------YK---------DRKPVFLGDPNIEVLNR--GQQLKIKSARLGDQARYQCSATNAAGKQSKDFNLSIFVPPSIKGGNVSSEVTVLLG-NLVTLECEVRGVPLPAVTWYKNGEVILSSRQAQ 1588          

HSP 5 Score: 88.1965 bits (217), Expect = 6.978e-17
Identity = 155/692 (22.40%), Postives = 270/692 (39.02%), Query Frame = 3
Query:  330 ESQITISHESRLTIHNISSDNSGYYVCRVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNA-IKRLKI--ISSNFPNSTI--AEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQWVIMQGLQIIDSSKF-----------IAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLIHIDS---NYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMTDSSAVLISDDAKLNFILIDR---SSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETK--------DIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLY-SRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLN---QNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQG--IKKVNFEVVFK 2297
            ES   +    ++ I N    ++G YVC  T +   + I     V+V  PP            V      + ++C  +G  P P I+   +KD   +K  +   I+SN     I  A+VS  +L+            C A N+AG   +   + ++   P I+   P   + +++ +I L C V   P  +  W +  G  +  + +F           I K R +  G   C A N  G  ++ ++L+V   P I       N  +       + C+ T +P P  + WYK      +SA  ++  ++ + + IDR   S  G Y CV  N+  ST         E T  L V   P      G  +++++  +   ++C    ++ G P P+L W+   +          ++S       I+++    G Y C+A N  G+   RE  L  V   P+I+ +  N  V +      QS+   C+              ++ IPP +  ++ +D R         ++ K  + +    +F +  S +    GRY CEA N  G     Y L +  PP    +IR     +SL      ++ + C++  I    + W K    +                 K +    + + G    +L I   E  D S+Y+C+AS+  G  IK+ N +V  +
Sbjct: 1679 ESLRVLDQGRKIEILNALQSDAGRYVCVATSVAGEKEI--KYDVSVLVPPVVDGASDTTDATVILNN-ILELECYATG-RPTPTITW--LKDGVPVKHGEGVRITSNGRRLVISRAQVSDTALFH-----------CVATNEAGEQEREFKV-SVHVPPAIRSTGPAERSVVLHTSISLQCVVSGIPPPSTTW-LKDGRPVDTTQEFLKLESAGRVLHIKKARLEDAGKYTCVATNAAGEAQQHIRLSVHEPPSIQYAGEMLNETILAGFQIQLKCKATGSPLPA-VTWYK-DGRPLTSAAGVNILSRGHVLEIDRAQVSDAGFYKCVAINVAGST---------ELTYSLQVYVPPTISSKGG--MVTVVVNDPVRLEC----EASGVPVPSLTWLKEGSPVSSFSDGIQVLSGGRVLSFISAQVSDAGHYTCVAVNAGGEQH-REYEL-RVYVPPNIMGEEVNNTVLMG-----QSVQLHCQ--------------SDAIPPPALSWR-KDGR--------PLYRKPGLSVSEDGSFLKIISAQVQDTGRYTCEATNVAGKTEKHYNLNVWVPP----SIRGSDEVSSLTVIEGGLITLVCESSGIPPPSLIWKKDGSEL-----------------KGDSRVRVLSGG---RQLQISSAEKADASSYTCLASSAAGSAIKEYNLQVYVR 2280          

HSP 6 Score: 85.8853 bits (211), Expect = 3.287e-16
Identity = 154/700 (22.00%), Postives = 254/700 (36.29%), Query Frame = 3
Query:  363 LTIHNISSDNSGYYVCRVTLLTTSEHIISLAVVTVKT----------------PPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPN-------TVNAMINQTIDLSCEVYANPAATIQW-------------VIMQGLQIIDSSKFIAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLIHIDSNYRVPK------NSNYSINCRVTSNPPPEFIKWYKLSN--MTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKW------IDVETKD-IVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQPD 2309
            L I N   +++G Y C    + T+E   +L    VK                  PK +    N ++ +  E +           IP P +  Y  KD  I +     +   N  I ++    +       + G   C A N AG   K+  + NI+  P  + F  +         + ++N  I L CE  A P   + W             +I+ G +++     I + + D  G   C A N  G       + VL  P I   ++  +P       N    + C+V  +P P+ I W K S     D++  L+S+   L  +    + TG Y CV QN+            +EK+  L V   P    I    +  ++    F+   S + ++ G P PTL+W      +   T   I+    T Q++ ++    GKY C+A N  G++  ++   +TV   PSI     +  V L+   + +S+   CE    P     W Y N +    + + +I                     L      Q      S  G+Y C+A N  G    ++ L I  PP +      R +      N V   C A  I    + W K N  ++   +S +  I                     ++L I   +  D   Y+CVASN +G  + N+ +V    P+
Sbjct: 2620 LQILNAKEEDAGRYTC----VATNEAGETLKNYEVKVFVPPVINKNDIPGESLAPKEVKIKVNNTLTLECEAQ----------AIPTPTLVWY--KDGQILKADGHLTITANGRIVQIKHTQV------SDTGRYTCVATNIAGEDEKDFDV-NIQVPPSFRGFGDSGPGNNGEVKDVILNNPISLYCETNAVPPPVLTWYKDGHLLTSNDKVLILPGGRVLQ----IPRAQVDDTGRYTCVAVNEAGEDSIQYDVRVLLPPSIR-GADGELPDEVTVLVNKTMLLECQVDGSPTPK-ISWVKDSQPLTQDNTHRLLSNGRTLQILNAQVTDTGRYVCVAQNLA---------GTAEKSFNLHVHVSPTIVGIREENVTVVLN--NFV---SLNCEATGLPPPTLRWFKDRRPVQASTNALIMPGGRTLQILKAKMSDGGKYSCVAVNPAGEA--QKLIYLTVYVPPSIRHNSGDLPVVLNV-QVGKSVMLECESNAVPPPVITW-YKNGRPIAETANLRI---------------------LADGQMLQLKETEVSDTGQYVCKATNVAGQVDKNFHLNIHVPPRLDGPAEERVVETI--SNPVTFACDATGIPPPSLTWLK-NGRVIENSESLEMHILSGG-----------------SKLQISRSQLSDSGTYTCVASNVEGKAQKNYHLVIHVPPN 3231          

HSP 7 Score: 85.8853 bits (211), Expect = 3.761e-16
Identity = 150/670 (22.39%), Postives = 262/670 (39.10%), Query Frame = 3
Query:  381 SSDNSGYYVCRVTLLTTSEHIISLAV-VTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNF---PNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQW--------VIMQGLQIIDSSKF-IAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLIHID-SNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNM-TDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKW--IDVETKDIVSD---METFQVI--NSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSI------ITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHG-IASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQ 2303
            S +++  Y C V+     E   S A+ +TV  PP   S     +  V      + I C+ SG +P P   +Y  KD    RL      F   P+  + E++A  L       + G   C A N AG   ++  L  +   P I+   P+T++ ++N  + L C    +P  TI W            G  ++ +    I+K + +  G  +C A+N  G+     +L V   P+I  D   Y V  + + ++ C     P P  + W+K   + ++S    +     L         TG Y C   N+           +S   + LTV   P     +G   +S+++  + ++ C  D    G P PT+ W   D+   D  ++   + T  ++   ++   +G Y C+  N +G+  SR  +L +++  P+       +  +K  ++ +            C   G P  +  W + N  +P         DH N  +E        L I+  S+  S          G Y C A+N  G I SL  F+ +K PPII  ++   +I   L  N ++  C+        I+W K   ++ +  + ++                     L    L I    ++D  NY CVA+ND G+ +    +  +S+
Sbjct: 3852 SVEDTALYECVVSNEAGQE---SRAIQLTVHVPP---SIADEATELVVSRLSPVVIGCTASG-VPHP--VLYWSKDGL--RLAKDGEGFTILPSGPL-EITAADL------SHSGRYSCVAKNAAGTAHRHVQL-TVHESPVIQSH-PSTLDVILNNHVTLPCRATGSPRPTITWQKEGINIFTTGGGFTVLPNGGLQISKAKVEDSGTYMCVAQNPAGTALGKTKLRVQVPPVITSDIRAYTVALDVSVTLQCNSEGFPTPS-VSWHKDGQLLSESVRQRVLSTGALQIAFAQPGDTGRYTCTVANVA---------GSSSLDISLTVQIPPS--IRVGESEVSVVENTQALLTCVAD----GVPQPTITWEKDDIPLTDTTAEYTILPTGDLLIDTAQPEDSGSYTCVGTNSMGQD-SRTISL-SIHTHPAFTELLGDVALNKRERLLM-----------VCGVTGIPPPKITWTFNNNIVPAQY------DHYNGHSE--------LVIERVSKDDS----------GTYTCMAENSVGSIKSLG-FVYVKEPPIIDGDVHSNRIEP-LGGNAIL-NCEVRGDPLPTIQWSKKGINVQISNRIRQ---------------------LDNGSLAIYGTVSEDAGNYMCVATNDAGVVERTVTLTLQSK 4424          

HSP 8 Score: 80.8777 bits (198), Expect = 1.139e-14
Identity = 138/647 (21.33%), Postives = 245/647 (37.87%), Query Frame = 3
Query:  537 IAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKI--KPFVPNTVNAMINQTIDLSCEVYANPAATIQWVIMQGLQIIDSSKFIAKIRPDMD-----------GPLICEAKNYLGSTKETVQLNVLRDPLIHIDS---NYRVPKNSNYSINCRVTSNPPPEFIKWYKLSN--MTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSP--------GEPFPTLKWIDVETKDIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTV------NEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNI-SNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXX-----XXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQPDPPYDLRVTDFEW-DSVTI 2360
            I ++C   G +P P +S        +K  + +S+      + E   + +     + + G   C AIN AG   +   + N+   P I  +  +P  ++ ++   + L+CE    P   I W  ++  Q I +S  +  I                G   C   N  G  ++   L VL  P I  +    + +V +  N  + C VT NP PE I W K      +D+   ++S+   L       + TG Y+C+  N    + G R   +    + ++ T     P+    ++   +     ++ C      P        G PF + + + V     +    T Q++N++    G+Y C+A NE G++       V V      N+ P      K  K+ ++      +++  CE +  P     W Y + +I       K + H  I +N RI QI              + T +  S  GRY C A N  G    D+ + I+               + ++   +  N + + C+  ++    + WYK + H++                    ++ L  PG +V  L I   + DD   Y+CVA N+ G   + ++V     P     +R  D E  D VT+
Sbjct: 2299 ITLRCEADG-VPRPAVSW-------MKDGRPLSTGRKAQILNEGRLLRILEAQ-VADTGRYTCIAINVAGQADRKYDV-NVHVPPTIIGQTHMPENISVVVKNPVVLTCEASGMPPPAITW--LKDGQPISTSSSVRVISGGRGLRLMHAASSDAGRYTCIVSNSAGEERKNFDLYVLVPPSIVNEGTVEDVKVKERQNAILACEVTGNPVPE-ITWLKDGQPLASDTRLQVMSNGRFLQISASQVADTGRYSCLASN----SAGDRS-RHFNLNVLVSPTIAGSGPEGSAEEVTVTLNSPTSLV-CEAQSYPPAIITWLKDGTPFESSRNVRV-----LPGGRTLQILNAKEEDAGRYTCVATNEAGETLKNYEVKVFVPPVINKNDIPGESLAPKEVKIKVN-----NTLTLECEAQAIPTPTLVW-YKDGQI------LKADGHLTITANGRIVQI--------------KHTQV--SDTGRYTCVATNIAGEDEKDFDVNIQVPPSFRGFGDSGPGNNGEVKDVILNNPISLYCETNAVPPPVLTWYK-DGHLLT-----------------SNDKVLILPGGRV--LQIPRAQVDDTGRYTCVAVNEAGEDSIQYDVRVLLPP----SIRGADGELPDEVTV 2869          

HSP 9 Score: 78.1814 bits (191), Expect = 6.845e-14
Identity = 152/754 (20.16%), Postives = 274/754 (36.34%), Query Frame = 3
Query:  345 ISHESRLTIHNISSDNSGYYVC-RVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKI-KPFVPNTVNAMINQTIDLSCEVYANPAATIQW-------VIMQGLQIIDSSKFIAKIRPDMD--GPLICEAKNYLGSTKETVQLNVLRDPLIHID---SNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSN--MTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWID-------VETKDIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSIS--------FTCEFRGQPLYEARW---------------------------------RY--------GNEK--------IPPS------SQDFKIEDHRN--ISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNG--------------RYNCEADNRHGIASLDY---FLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDL-FTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEV 2288
            +S    L I      ++G+Y C  + +  ++E   SL V      P +IS        V  +   + ++C  SG +P P ++        +K    +SS      +     +  +    + + G   C A+N  G   +   L  +   P I    V NTV  ++ Q++ L C+  A P   + W           GL + +   F+  I   +   G   CEA N  G T++   LNV   P I      S+  V +    ++ C  +  PPP  I W K  +    DS   ++S   +L     +++    Y C+  +   S     K  N +  +  +++   ++ +       ++ +G    ++C  D    G P P + W+             I+++    +++ ++    G+Y C+A N  G++  +    V V+  P+II Q          + +P++IS         TCE  G P     W                                 RY        G E+        +PPS       +D K+++ +N  ++ E       ++T     +  +  T L+   NG              RY+C A N  G  S  +    L+              ++  +LN    ++ C+A+S     I W K                   + T +E  R++   PG +   L I + + +D   Y+CVA+N+ G    N+EV
Sbjct: 1963 LSRGHVLEIDRAQVSDAGFYKCVAINVAGSTELTYSLQVYV----PPTISSKGGMVTVVVNDP--VRLECEASG-VPVPSLTW-------LKEGSPVSSFSDGIQVLSGGRVLSFISAQVSDAGHYTCVAVNAGGEQHREYEL-RVYVPPNIMGEEVNNTV--LMGQSVQLHCQSDAIPPPALSWRKDGRPLYRKPGLSVSEDGSFLKIISAQVQDTGRYTCEATNVAGKTEKHYNLNVWVPPSIRGSDEVSSLTVIEGGLITLVCESSGIPPPSLI-WKKDGSELKGDSRVRVLSGGRQLQISSAEKADASSYTCLASSAAGSAI---KEYNLQVYVRPSISSSAQDEE-------TVTRGGDITLRCEAD----GVPRPAVSWMKDGRPLSTGRKAQILNEGRLLRILEAQVADTGRYTCIAINVAGQADRKYD--VNVHVPPTIIGQ----------THMPENISVVVKNPVVLTCEASGMPPPAITWLKDGQPISTSSSVRVISGGRGLRLMHAASSDAGRYTCIVSNSAGEERKNFDLYVLVPPSIVNEGTVEDVKVKERQNAILACEVTGNPVPEITWLKDGQPLASDTRLQVMSNGRFLQISASQVADTGRYSCLASNSAGDRSRHFNLNVLVSPTIAGSGPEGSAEEVTVTLNSPTSLV-CEAQSYPPAIITWLK-------------------DGTPFESSRNVRVLPGGRT--LQILNAKEEDAGRYTCVATNEAGETLKNYEV 2650          

HSP 10 Score: 75.8702 bits (185), Expect = 3.943e-13
Identity = 141/619 (22.78%), Postives = 225/619 (36.35%), Query Frame = 3
Query:  537 IAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTI------DLSCEVYANPAATIQW-------VIMQGLQIIDSSKFIAKIRPDMD--GPLICEAKNYLGSTKETVQLNVLRDPLIHIDSNY-RVPKNSNYSINCRVTSNPPPEFIKWYK----LSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKII-SIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETKD-----------IVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNE---LTIKDLENDDFSNYSCVASNDQGIKKVNFEV 2288
            I++QC VSG IPPP  S   +KD   + +          +   V  I   R   LE+ G   C A N AG   ++  L ++   P I+         M+N+TI       L C+   +P   + W           G+ I+     +   R  +   G   C A N  GST+ T  L V   P I        V  N    + C  +  P P  + W K    +S+ +D   VL S    L+FI    S  G Y CV  N              +   +    Y P  P I+G ++  +++ G+   + C +D   P    P L W     KD           +  D    ++I+++ +  G+Y C A N  GK+       V V   PSI    +   + +    L   I+  CE  G P     W+    ++     D ++   R +S  R  QI +    D  S                Y C A +  G A  +Y L +   P I  + +  +         + +RC+A+ +    + W K       P  + +    +A++               +NE   L I + +  D   Y+C+A N  G     ++V
Sbjct: 1837 ISLQCVVSG-IPPP--STTWLKDG--RPVDTTQEFLKLESAGRVLHIKKAR---LEDAGKYTCVATNAAGEAQQHIRL-SVHEPPSIQ-----YAGEMLNETILAGFQIQLKCKATGSPLPAVTWYKDGRPLTSAAGVNILSRGHVLEIDRAQVSDAGFYKCVAINVAGSTELTYSLQVYVPPTISSKGGMVTVVVNDPVRLECEASGVPVPS-LTWLKEGSPVSSFSDGIQVL-SGGRVLSFISAQVSDAGHYTCVAVNA----------GGEQHREYELRVYVP--PNIMGEEVNNTVLMGQSVQLHCQSDAIPP----PALSW----RKDGRPLYRKPGLSVSEDGSFLKIISAQVQDTGRYTCEATNVAGKTEKHYNLNVWV--PPSIRGSDEVSSLTVIEGGL---ITLVCESSGIPPPSLIWKKDGSEL---KGDSRV---RVLSGGRQLQISSAEKADASS----------------YTCLASSAAGSAIKEYNLQVYVRPSISSSAQDEETVTRGGD--ITLRCEADGVPRPAVSWMKDGR----PLSTGR----KAQI---------------LNEGRLLRILEAQVADTGRYTCIAINVAGQADRKYDV 2367          

HSP 11 Score: 75.485 bits (184), Expect = 5.663e-13
Identity = 134/634 (21.14%), Postives = 237/634 (37.38%), Query Frame = 3
Query:  327 SESQITISHESRLTIHNISSDNSGYYVCRVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQWVIMQGLQIIDSSKFIAKIRPDMDGPL-------------ICEAKNYLG--STKETVQLNVLRDPLIHIDSNY-RVPKNSNYSINC-RVTSNPPPEFIKWYKLSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKW------IDVETKDI-VSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQ---HKNRKVFLSYSDL-PQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVT 2144
              S+  ++ +  L I N    ++G Y C  + +  ++     A+V+    P+    H +  I + Q+     ++C V+G +P P I M+   D  +K   ++S +    T+            Y        C A+N AG   +    L++   P+     P+ ++A I   I L C    +P   + W     + +       + +   MDG L             +CEA+N+ G    K  V +  L  PLI +      V ++   ++ C  +  NP PE    +    +T S  V +  D  L+   + +   G Y C+T+N+           +S +T  L V   P      G +I S ++G    + C    ++ G P P + W      +D+      +++  +  + +     +G++ C A N  G S  +    V V    S + +   H N +  L  S +    I+  CE    P     W    + I P                     F+   I L S +  + T  R + +G Y C A N  G  S    L +  PP I    R  K+      + + + C  + +    + W K  +  VL   S +Y I +  +T
Sbjct:  646 GSSRHRMTPDGTLIIKNTGLKDAGTYGCLASNVAGTDS--QTAIVSYIESPQVTVPHSDLLIGLGQK---TVMECRVTG-VPHPDI-MWYKGDLQLKPSSVLSMDPQRGTLTIQQTQDTHAGQY-------TCVAVNSAGS-AQGYITLDVGSAPQFTKK-PSDLSADIGTNITLPCYAQGHPKPQLSWRREDNIALFTQRHGPSTVTHRMDGGLFITNLWVEDEATYVCEAQNHFGRIQVKAKVTVTGLVSPLIALSPTVVSVIEDQQVTLPCVLLAGNPLPERHWLHDNGLVTSSPYVTVRRDGSLHIEQVSQQDGGQYTCLTENVV---------GSSNRTTILNVYVMPTIQH--GPQIFSTIEGTPISLPC----RAHGVPKPDITWSKRGELLDLSGSVFSLTEDGSLHIHSPSGEESGEFVCTATNAAGYSSRKVQLTVYVRPKLSGVGEADIHDNSEKLLEMSVIVGDDITLPCEVESVPPPIITWAKDKQLISP---------------------FSPRHIQLPSGSM-RITDTRVTDSGMYLCVATNIAGNFSQTVKLSVLVPPSISAGPRAMKVQIG---HAIDLPCVTQGVPEPSVSWLK--DGTVLQDGS-RYRISDGALT 1220          

HSP 12 Score: 67.781 bits (164), Expect = 1.073e-10
Identity = 82/364 (22.53%), Postives = 138/364 (37.91%), Query Frame = 3
Query:  699 LENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQWVIMQGLQIIDS--------SKFIAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLIHIDSNYRVPKNSNYS------INCRVTSNPPPEFIKWYKLSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKW----IDVETKDIVS---DMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWR 1727
            L++ GF  C AI+ AG     T L   +  P I   VP    A     + L+C V +     + W        +D         S  I ++ PD  G   C A N  G +   V LNV   P++ +D     P+N  +       I C     P P  +  +    +  SS   ++ D  L          G Y C+  N+              +T  ++    P+      + +I +  G+K +++C    +  G P P + W    + ++   ++S      T  +  ++  H G+Y C+A N  G +    T  + V  AP    Q   +   LS +D+  +I+  C  +G P  +  WR
Sbjct:  485 LKDEGFYECIAISSAGTGRAQTFLDVSEPPPAIT--VPFNATASPGANVILTCLVTSTVRFNLTWQRGDVDARLDHRIRVTTNLSLEIQRVTPDDVGWYTCIAANEGGVSAARVYLNVQEPPVVTVD-----PRNQTFQTGQEIWIRCSAKGYPSPMVVWTHNDMFIMGSSRHRMTPDGTLIIKNTGLKDAGTYGCLASNVA---------GTDSQTAIVSYIESPQVTVPHSDLLIGL--GQKTVMEC----RVTGVPHPDIMWYKGDLQLKPSSVLSMDPQRGTLTIQQTQDTHAGQYTCVAVNSAGSAQGYIT--LDVGSAP----QFTKKPSDLS-ADIGTNITLPCYAQGHPKPQLSWR 819          

HSP 13 Score: 62.7734 bits (151), Expect = 3.495e-9
Identity = 102/437 (23.34%), Postives = 178/437 (40.73%), Query Frame = 3
Query:  363 LTIHNISSDNSGYYVC-RVTLLTTSEHIISLAVVTVKTPP--KSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPN-TVNAMINQTIDLSCEVYANPAATIQW---------------VIMQGLQI-IDSSKFIAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLI---HIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMTDSS--AVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQIL----GNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWID-------VETKDIVSDMETFQVINSESRHNGKYKCLAENEIGK 1565
            L + ++S  ++G Y C  V    T+E    L V     PP  +      N S+ + Q    +   C V+G  P P I+ Y  KD     ++++ S+  +  I +           +++ G  +C AIN AG   ++   L++   P I   V +  ++A++NQ I L C+V  +P  TIQW               V+ +G Q+ I S++   + R        C A N  G   +   L++   P I   ++ S   V   +  ++ C V   P P  + WYK   +  SS  A  +     L  +    S  G Y C   ++            +EK + L V Y P  P I     G   + ++  +  I++C  +    G P P+L W+         E+  ++      +++N+     G+Y C+A +  G+
Sbjct: 1316 LILASVSPLDNGEYTCMAVNDAGTTEKKYQLKV---NVPPDIRDNGLLSNVSVVINQPTNLV---CDVTG-TPVPVITWY--KDG----VEVVPSS--DVQILQKGKTLKLLKAAVQDAGRYICKAINIAGSRERDF-YLDVLVPPTIIGTVGSRDLSAVLNQEIVLECKVKGDPFPTIQWYKDRKPVFLGDPNIEVLNRGQQLKIKSARLGDQAR------YQCSATNAAGKQSKDFNLSIFVPPSIKGGNVSSEVTVLLGNLVTLECEVRGVPLPA-VTWYKNGEVILSSRQAQYVDRGQFLKILRAQVSDAGQYTCRVTSVA---------GTAEKVVELDV-YVP--PSITAGSDGPTDMKVVLNKSLILECEAE----GHPPPSLTWLKDGSPVAVRESLRVLDQGRKIEILNALQSDAGRYVCVATSVAGE 1713          
BLAST of hemicentin-1 isoform X2 vs. Ensembl Zebrafish
Match: hmcn1 (hemicentin 1 [Source:ZFIN;Acc:ZDB-GENE-041014-322])

HSP 1 Score: 110.153 bits (274), Expect = 1.561e-23
Identity = 157/688 (22.82%), Postives = 283/688 (41.13%), Query Frame = 3
Query:  327 SESQITISHESRLT-IHNISSDNSGYYVCRVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFI-----AIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSN-----FPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPF---VPNTVNAMINQTIDLSCEVYANPAATIQWVI-MQGLQIIDSSKFIAKIRPDM--------DGPLICEAKNYLGSTKETVQLNVLRDPLIHI--DSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMTDSS--AVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPAN-SEKTMFLTVTYKPKEPQILGN--KIISIMKGEKFIIKCSTDRKSPGEPFPTLKWID-----VETKD--IVSDMETFQVINSESRHNGKYKCLAENEIG---KSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRS--SPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQG 2264
            ++  +TI+   R+  I +    ++G Y C  T +   +       V ++ PP    F  +   N  + ++ I     ++ C  +  +PPP ++ Y       K   +++SN      P   + ++    +      ++ G   C A+N+AG  +    +  +   P I+     +P+ V  ++N+T+ L C+V  +P   I WV   Q L   ++ + ++  R            G  +C A+N  G+ +++  L+V   P I    + N  V  N+  S+NC  T  PPP  ++W+K      +S  A+++     L  +    S  G Y+CV  N          PA  ++K ++LTV   P      G+   ++++  G+  +++C ++   P    P + W        ET +  I++D +  Q+  +E    G+Y C A N  G   K+F      + ++  P +    + R V      +   ++F C+  G              IPP S  +       + N R+ +    L + + S     Q S RS  S +G Y C A N  G A  +Y L+I  PP I  +    +++  LN +V ++ C+AE   T  I+W K    I    Q            K  P+          + LT+  +   D   Y+CVA+N  G
Sbjct: 2705 ADGHLTITANGRIVQIKHTQVSDTGRYTCVATNIAGEDE--KDFDVNIQVPPSFRGFGDSGPGNNGEVKDVILNNPISLYCETNA-VPPPVLTWY-------KDGHLLTSNDKVLILPGGRVLQIPRAQV------DDTGRYTCVAVNEAGEDSIQYDV-RVLLPPSIRGADGELPDEVTVLVNKTMLLECQVDGSPTPKISWVKDSQPLTQDNTHRLLSNGRTLQILNAQVTDTGRYVCVAQNLAGTAEKSFNLHVHVSPTIVGIREENVTVVLNNFVSLNCEATGLPPPT-LRWFKDRRPVQASTNALIMPGGRTLQILKAKMSDGGKYSCVAVN----------PAGEAQKLIYLTVYVPPSIRHNSGDLPVVLNVQVGKSVMLECESNAVPP----PVITWYKNGRPIAETANLRILADGQMLQLKETEVSDTGQYVCKATNVAGQVDKNFH-----LNIHVPPRLDGPAEERVV----ETISNPVTFACDATG--------------IPPPSLTW-------LKNGRVIENSESLEMHILSGGSKLQIS-RSQLSDSGTYTCVASNVEGKAQKNYHLVIHVPPNIIGSELPSEMSVLLNDSVQLV-CRAEGTPTPEIQWLKDGMTISRTAQKN---------IKISPDG---------STLTVTAVHTSDSGKYTCVATNQAG 3310          

HSP 2 Score: 100.138 bits (248), Expect = 1.543e-20
Identity = 152/680 (22.35%), Postives = 251/680 (36.91%), Query Frame = 3
Query:  363 LTIHNISSDNSGYYVCRVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKII--SSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKI-KPFVPNTVNAMINQTIDLSCEVYANPAATIQWVIMQGLQIIDSSKFIAKIRPDMD------------GPLICEAKNYLGSTKETVQLNVLRDPLIHIDSNYRVPK------NSNYSINCRVTSNPPPEFIKWYK--LSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVE---TKD----IVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPG--LKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQP 2306
            L +      ++G YVC+ T    +  +     + +  PP+       + +        +   C  +G IPPP ++        +K  ++I  S +     ++  S + + R   L + G   C A N  G   KN  L+ I   P I    +P+ ++ ++N ++ L C     P   IQW +  G+ I  +++   KI PD              G   C A N  G       LNV   PLI    N    K      +S+ +I C  + +PPP+ + W K  L     S   L+S    L       S  G Y CV  N         +     +   L V   P        + ++++KG      C  D    G P P + W+      +KD    +++   T Q++N    H G+Y C A N+ G + SR  +L  V + P I       +V +  + +   +   CE  G PL    W      +P +          NI   R  Q+          R  S Q        GRY C A +  G    + FL+  + P      R  +  + L    V + C+++++    + W K +  +                 K  P   + + G  L++N   +      D + YSCVASN  G  + +F++     P
Sbjct: 3099 LQLKETEVSDTGQYVCKAT--NVAGQVDKNFHLNIHVPPRLDGPAEERVVETISNP--VTFACDATG-IPPPSLTW-------LKNGRVIENSESLEMHILSGGSKLQISRSQ-LSDSGTYTCVASNVEGKAQKNYHLV-IHVPPNIIGSELPSEMSVLLNDSVQLVCRAEGTPTPEIQW-LKDGMTISRTAQKNIKISPDGSTLTVTAVHTSDSGKYTCVATNQAGEEDRIFNLNVYVPPLIQ--GNGPAAKELTTVLDSSINIECVASGSPPPQ-LNWLKNGLPLPVSSQIRLLSAGQVLRIARAQVSDGGSYTCVASN---------RAGVDNRHYTLQVYVPPSLDGAGSTEEVTVVKGSTASFICIAD----GTPSPVIIWLRTGASVSKDAHISLLNQNSTMQIVNVLVNHTGRYTCTAHNQAGDA-SRHFSL-KVLDPPRINGSGVPAEVSVVVNHV---LELLCEADGIPLPTLTWLKDGRPLPQT---------ENIRLLRDGQML---------RVASAQV----ENTGRYTCLASSPAGDDDKE-FLVRVHVPPNIAGDRGVQDVSVLQNRQVTLECKSDAVPPPTLTWLKDDAPL-----------------KMSPRVRVLSSGRYLQINNAVL-----GDGAQYSCVASNVAGETRRHFKLSVNVPP 3697          

HSP 3 Score: 93.5893 bits (231), Expect = 1.536e-18
Identity = 152/708 (21.47%), Postives = 265/708 (37.43%), Query Frame = 3
Query:  318 VPDSESQITISHESRLTIHNISSDNSGYYVCRVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIK---RLKIISSN----FPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQWVIMQGLQII----------DSSKFIAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLI-HIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYK----LSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETKD---IVSDMETFQVINS--------ESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQPDPPYDLRVTDFE 2342
            +P +E+   +     L + +   +N+G Y C  +     +    L  V V  PP        + ++V Q ++ + ++C  S  +PPP ++ ++  D  +K   R++++SS       N+ + + +  S              C A N AG  T+    L++   P IK   P +V+  INQ + L C V   PA  I W   QG  +           D S  I   +    G  +C A N  G+ ++ V L V   P I    +N  V  N   +++C  T  P P  + W K    L+   + +   +     L  I      T +Y CV  N         +     + + LTV   P         ++S +     +I C+      G P P L W    +KD   +  D E F ++ S        +  H+G+Y C+A+N  G +  R   L TV+E+P I +      V L+       ++  C   G P     W+     I  +   F +  +  +                      Q +  +   +G Y C A N  G A     L ++ PP+I  +IR   +A  ++   V ++C +E   T  + W+K  + +    + +  S    ++   +P                      D   Y+C  +N  G   ++  +  +  P     +RV + E
Sbjct: 3553 LPQTENIRLLRDGQMLRVASAQVENTGRYTCLASSPAGDDDKEFL--VRVHVPPNIAGDRGVQDVSVLQNRQ-VTLECK-SDAVPPPTLT-WLKDDAPLKMSPRVRVLSSGRYLQINNAVLGDGAQYS--------------CVASNVAGE-TRRHFKLSVNVPPTIKDG-PQSVSVHINQPVVLECVVNGVPAPRITWR-KQGSILAGNNPRYKFGEDGSLRILSAQVTDTGRYLCMATNQAGTERKRVDLQVYVPPSIAEGPTNITVTVNVQTTLSCEATGIPKPS-VSWTKNTQALNTDQNQNMYRLLSSGSLVVIAPSVEDTALYECVVSN---------EAGQESRAIQLTVHVPPSIADEATELVVSRLS--PVVIGCTAS----GVPHPVLYW----SKDGLRLAKDGEGFTILPSGPLEITAADLSHSGRYSCVAKNAAGTAH-RHVQL-TVHESPVIQSHPSTLDVILN-----NHVTLPCRATGSPRPTITWQKEGINIFTTGGGFTVLPNGGL----------------------QISKAKVEDSGTYMCVAQNPAGTALGKTKLRVQVPPVITSDIRAYTVALDVS---VTLQCNSEGFPTPSVSWHKDGQLLSESVRQRVLSTGALQIAFAQP---------------------GDTGRYTCTVANVAGSSSLDISLTVQIPP----SIRVGESE 4161          

HSP 4 Score: 88.1965 bits (217), Expect = 6.978e-17
Identity = 151/635 (23.78%), Postives = 247/635 (38.90%), Query Frame = 3
Query:  327 SESQITISHESRLTIHNISSDNSGYYVCRVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQ---------EFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQW-----VIMQG--LQIIDSSKFIAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLIHI-DSNYRVP-----KNSNYSINCRVTSNPPPEFIKWYKLSN--MTDSSAVLISDDAKLNFILIDRS--STGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKP--KEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWID-----VETKD--IVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETAL-VTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNG---RYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSK 2114
            S S  +++ +  L IH+ S + SG +VC  T    + +      +TV   PK +S      I+   E+         + I + C V   +PPP I+    KD      K + S F    I   S         + + G  +C A N AG  ++ T  L++   P I    P  +   I   IDL C     P  ++ W     V+  G   +I D +  + ++    +G  +C A N  G  +  +QL+V   P++ + +  +  P      N   +  C     P P  IKW++  +    +   V I +D  L  IL   S    G Y C+  N             +EK   L V   P  ++  +L N  + I +    +   +      G P P + W       V + D  I+   +T +++ +  +  G+Y C A N  G   SRE    + V   P+II    +R +    + L Q I   C+ +G P    +W             +K         +R         I++ +R   QQ  ++S+  G   RY C A N  G  S D+ L I  PP IK      ++   L  N+V + C+   +    + WYK  E I+   Q++
Sbjct: 1015 SGSVFSLTEDGSLHIHSPSGEESGEFVCTAT--NAAGYSSRKVQLTVYVRPK-LSGVGEADIHDNSEKLLEMSVIVGDDITLPCEVES-VPPPIITW--AKD------KQLISPFSPRHIQLPSGSMRITDTRVTDSGMYLCVATNIAGNFSQ-TVKLSVLVPPSISAG-PRAMKVQIGHAIDLPCVTQGVPEPSVSWLKDGTVLQDGSRYRISDGALTLNQVALTDEGIYVCRAVNIAGKEETAIQLHVQVPPVVEVSEPPFNSPLQERVANQQIAFPCPAKGTPEP-VIKWFRNGHELTGNEPGVSILEDGTL-LILASVSPLDNGEYTCMAVN---------DAGTTEKKYQLKVNVPPDIRDNGLLSNVSVVINQPTNLVCDVT------GTPVPVITWYKDGVEVVPSSDVQILQKGKTLKLLKAAVQDAGRYICKAINIAG---SRERDFYLDVLVPPTIIGTVGSRDL---SAVLNQEIVLECKVKGDPFPTIQW-------------YK---------DRKPVFLGDPNIEVLNR--GQQLKIKSARLGDQARYQCSATNAAGKQSKDFNLSIFVPPSIKGGNVSSEVTVLLG-NLVTLECEVRGVPLPAVTWYKNGEVILSSRQAQ 1587          

HSP 5 Score: 88.1965 bits (217), Expect = 7.339e-17
Identity = 155/692 (22.40%), Postives = 270/692 (39.02%), Query Frame = 3
Query:  330 ESQITISHESRLTIHNISSDNSGYYVCRVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNA-IKRLKI--ISSNFPNSTI--AEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQWVIMQGLQIIDSSKF-----------IAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLIHIDS---NYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMTDSSAVLISDDAKLNFILIDR---SSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETK--------DIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLY-SRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLN---QNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQG--IKKVNFEVVFK 2297
            ES   +    ++ I N    ++G YVC  T +   + I     V+V  PP            V      + ++C  +G  P P I+   +KD   +K  +   I+SN     I  A+VS  +L+            C A N+AG   +   + ++   P I+   P   + +++ +I L C V   P  +  W +  G  +  + +F           I K R +  G   C A N  G  ++ ++L+V   P I       N  +       + C+ T +P P  + WYK      +SA  ++  ++ + + IDR   S  G Y CV  N+  ST         E T  L V   P      G  +++++  +   ++C    ++ G P P+L W+   +          ++S       I+++    G Y C+A N  G+   RE  L  V   P+I+ +  N  V +      QS+   C+              ++ IPP +  ++ +D R         ++ K  + +    +F +  S +    GRY CEA N  G     Y L +  PP    +IR     +SL      ++ + C++  I    + W K    +                 K +    + + G    +L I   E  D S+Y+C+AS+  G  IK+ N +V  +
Sbjct: 1678 ESLRVLDQGRKIEILNALQSDAGRYVCVATSVAGEKEI--KYDVSVLVPPVVDGASDTTDATVILNN-ILELECYATG-RPTPTITW--LKDGVPVKHGEGVRITSNGRRLVISRAQVSDTALFH-----------CVATNEAGEQEREFKV-SVHVPPAIRSTGPAERSVVLHTSISLQCVVSGIPPPSTTW-LKDGRPVDTTQEFLKLESAGRVLHIKKARLEDAGKYTCVATNAAGEAQQHIRLSVHEPPSIQYAGEMLNETILAGFQIQLKCKATGSPLPA-VTWYK-DGRPLTSAAGVNILSRGHVLEIDRAQVSDAGFYKCVAINVAGST---------ELTYSLQVYVPPTISSKGG--MVTVVVNDPVRLEC----EASGVPVPSLTWLKEGSPVSSFSDGIQVLSGGRVLSFISAQVSDAGHYTCVAVNAGGEQH-REYEL-RVYVPPNIMGEEVNNTVLMG-----QSVQLHCQ--------------SDAIPPPALSWR-KDGR--------PLYRKPGLSVSEDGSFLKIISAQVQDTGRYTCEATNVAGKTEKHYNLNVWVPP----SIRGSDEVSSLTVIEGGLITLVCESSGIPPPSLIWKKDGSEL-----------------KGDSRVRVLSGG---RQLQISSAEKADASSYTCLASSAAGSAIKEYNLQVYVR 2279          

HSP 6 Score: 85.8853 bits (211), Expect = 3.576e-16
Identity = 154/700 (22.00%), Postives = 254/700 (36.29%), Query Frame = 3
Query:  363 LTIHNISSDNSGYYVCRVTLLTTSEHIISLAVVTVKT----------------PPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPN-------TVNAMINQTIDLSCEVYANPAATIQW-------------VIMQGLQIIDSSKFIAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLIHIDSNYRVPK------NSNYSINCRVTSNPPPEFIKWYKLSN--MTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKW------IDVETKD-IVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQPD 2309
            L I N   +++G Y C    + T+E   +L    VK                  PK +    N ++ +  E +           IP P +  Y  KD  I +     +   N  I ++    +       + G   C A N AG   K+  + NI+  P  + F  +         + ++N  I L CE  A P   + W             +I+ G +++     I + + D  G   C A N  G       + VL  P I   ++  +P       N    + C+V  +P P+ I W K S     D++  L+S+   L  +    + TG Y CV QN+            +EK+  L V   P    I    +  ++    F+   S + ++ G P PTL+W      +   T   I+    T Q++ ++    GKY C+A N  G++  ++   +TV   PSI     +  V L+   + +S+   CE    P     W Y N +    + + +I                     L      Q      S  G+Y C+A N  G    ++ L I  PP +      R +      N V   C A  I    + W K N  ++   +S +  I                     ++L I   +  D   Y+CVASN +G  + N+ +V    P+
Sbjct: 2619 LQILNAKEEDAGRYTC----VATNEAGETLKNYEVKVFVPPVINKNDIPGESLAPKEVKIKVNNTLTLECEAQ----------AIPTPTLVWY--KDGQILKADGHLTITANGRIVQIKHTQV------SDTGRYTCVATNIAGEDEKDFDV-NIQVPPSFRGFGDSGPGNNGEVKDVILNNPISLYCETNAVPPPVLTWYKDGHLLTSNDKVLILPGGRVLQ----IPRAQVDDTGRYTCVAVNEAGEDSIQYDVRVLLPPSIR-GADGELPDEVTVLVNKTMLLECQVDGSPTPK-ISWVKDSQPLTQDNTHRLLSNGRTLQILNAQVTDTGRYVCVAQNLA---------GTAEKSFNLHVHVSPTIVGIREENVTVVLN--NFV---SLNCEATGLPPPTLRWFKDRRPVQASTNALIMPGGRTLQILKAKMSDGGKYSCVAVNPAGEA--QKLIYLTVYVPPSIRHNSGDLPVVLNV-QVGKSVMLECESNAVPPPVITW-YKNGRPIAETANLRI---------------------LADGQMLQLKETEVSDTGQYVCKATNVAGQVDKNFHLNIHVPPRLDGPAEERVVETI--SNPVTFACDATGIPPPSLTWLK-NGRVIENSESLEMHILSGG-----------------SKLQISRSQLSDSGTYTCVASNVEGKAQKNYHLVIHVPPN 3230          

HSP 7 Score: 82.0333 bits (201), Expect = 4.665e-15
Identity = 150/670 (22.39%), Postives = 262/670 (39.10%), Query Frame = 3
Query:  381 SSDNSGYYVCRVTLLTTSEHIISLAV-VTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNF---PNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQW--------VIMQGLQIIDSSKF-IAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLIHID-SNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSN-MTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKW--IDVETKDIVSD---METFQVI--NSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSI------ITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHG-IASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQ 2303
            S +++  Y C V+     E   S A+ +TV  PP   S     +  V      + I C+ SG +P P   +Y  KD    RL      F   P+  + E++A  L       + G   C A N AG   ++  L  +   P I+   P+T++ ++N  + L C    +P  TI W            G  ++ +    I+K + +  G  +C A+N  G+     +L V   P+I  D   Y V  + + ++ C     P P  + W+K    +++S    +     L         TG Y C   N+           +S   + LTV   P     +G   +S+++  + ++ C  D    G P PT+ W   D+   D  ++   + T  ++   ++   +G Y C+  N +G+  SR  +L +++  P+       +  +K  ++ +            C   G P  +  W + N  +P         DH N  +E        L I+  S+  S          G Y C A+N  G I SL  F+ +K PPII  ++   +I   L  N ++  C+        I+W K   ++ +  + ++                     L    L I    ++D  NY CVA+ND G+ +    +  +S+
Sbjct: 3851 SVEDTALYECVVSNEAGQE---SRAIQLTVHVPP---SIADEATELVVSRLSPVVIGCTASG-VPHP--VLYWSKDGL--RLAKDGEGFTILPSGPL-EITAADL------SHSGRYSCVAKNAAGTAHRHVQL-TVHESPVIQSH-PSTLDVILNNHVTLPCRATGSPRPTITWQKEGINIFTTGGGFTVLPNGGLQISKAKVEDSGTYMCVAQNPAGTALGKTKLRVQVPPVITSDIRAYTVALDVSVTLQCNSEGFPTPS-VSWHKDGQLLSESVRQRVLSTGALQIAFAQPGDTGRYTCTVANVA---------GSSSLDISLTVQIPPS--IRVGESEVSVVENTQALLTCVAD----GVPQPTITWEKDDIPLTDTTAEYTILPTGDLLIDTAQPEDSGSYTCVGTNSMGQD-SRTISL-SIHTHPAFTELLGDVALNKRERLLM-----------VCGVTGIPPPKITWTFNNNIVPAQY------DHYNGHSE--------LVIERVSKDDS----------GTYTCMAENSVGSIKSLG-FVYVKEPPIIDGDVHSNRIEP-LGGNAIL-NCEVRGDPLPTIQWSKKGINVQISNRIRQ---------------------LDNGSLAIYG-TSEDAGNYMCVATNDAGVVERTVTLTLQSK 4422          

HSP 8 Score: 80.8777 bits (198), Expect = 1.208e-14
Identity = 138/647 (21.33%), Postives = 245/647 (37.87%), Query Frame = 3
Query:  537 IAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKI--KPFVPNTVNAMINQTIDLSCEVYANPAATIQWVIMQGLQIIDSSKFIAKIRPDMD-----------GPLICEAKNYLGSTKETVQLNVLRDPLIHIDS---NYRVPKNSNYSINCRVTSNPPPEFIKWYKLSN--MTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSP--------GEPFPTLKWIDVETKDIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTV------NEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNI-SNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXX-----XXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQPDPPYDLRVTDFEW-DSVTI 2360
            I ++C   G +P P +S        +K  + +S+      + E   + +     + + G   C AIN AG   +   + N+   P I  +  +P  ++ ++   + L+CE    P   I W  ++  Q I +S  +  I                G   C   N  G  ++   L VL  P I  +    + +V +  N  + C VT NP PE I W K      +D+   ++S+   L       + TG Y+C+  N    + G R   +    + ++ T     P+    ++   +     ++ C      P        G PF + + + V     +    T Q++N++    G+Y C+A NE G++       V V      N+ P      K  K+ ++      +++  CE +  P     W Y + +I       K + H  I +N RI QI              + T +  S  GRY C A N  G    D+ + I+               + ++   +  N + + C+  ++    + WYK + H++                    ++ L  PG +V  L I   + DD   Y+CVA N+ G   + ++V     P     +R  D E  D VT+
Sbjct: 2298 ITLRCEADG-VPRPAVSW-------MKDGRPLSTGRKAQILNEGRLLRILEAQ-VADTGRYTCIAINVAGQADRKYDV-NVHVPPTIIGQTHMPENISVVVKNPVVLTCEASGMPPPAITW--LKDGQPISTSSSVRVISGGRGLRLMHAASSDAGRYTCIVSNSAGEERKNFDLYVLVPPSIVNEGTVEDVKVKERQNAILACEVTGNPVPE-ITWLKDGQPLASDTRLQVMSNGRFLQISASQVADTGRYSCLASN----SAGDRS-RHFNLNVLVSPTIAGSGPEGSAEEVTVTLNSPTSLV-CEAQSYPPAIITWLKDGTPFESSRNVRV-----LPGGRTLQILNAKEEDAGRYTCVATNEAGETLKNYEVKVFVPPVINKNDIPGESLAPKEVKIKVN-----NTLTLECEAQAIPTPTLVW-YKDGQI------LKADGHLTITANGRIVQI--------------KHTQV--SDTGRYTCVATNIAGEDEKDFDVNIQVPPSFRGFGDSGPGNNGEVKDVILNNPISLYCETNAVPPPVLTWYK-DGHLLT-----------------SNDKVLILPGGRV--LQIPRAQVDDTGRYTCVAVNEAGEDSIQYDVRVLLPP----SIRGADGELPDEVTV 2868          

HSP 9 Score: 78.1814 bits (191), Expect = 7.199e-14
Identity = 152/754 (20.16%), Postives = 274/754 (36.34%), Query Frame = 3
Query:  345 ISHESRLTIHNISSDNSGYYVC-RVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKI-KPFVPNTVNAMINQTIDLSCEVYANPAATIQW-------VIMQGLQIIDSSKFIAKIRPDMD--GPLICEAKNYLGSTKETVQLNVLRDPLIHID---SNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSN--MTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWID-------VETKDIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSIS--------FTCEFRGQPLYEARW---------------------------------RY--------GNEK--------IPPS------SQDFKIEDHRN--ISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNG--------------RYNCEADNRHGIASLDY---FLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDL-FTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEV 2288
            +S    L I      ++G+Y C  + +  ++E   SL V      P +IS        V  +   + ++C  SG +P P ++        +K    +SS      +     +  +    + + G   C A+N  G   +   L  +   P I    V NTV  ++ Q++ L C+  A P   + W           GL + +   F+  I   +   G   CEA N  G T++   LNV   P I      S+  V +    ++ C  +  PPP  I W K  +    DS   ++S   +L     +++    Y C+  +   S     K  N +  +  +++   ++ +       ++ +G    ++C  D    G P P + W+             I+++    +++ ++    G+Y C+A N  G++  +    V V+  P+II Q          + +P++IS         TCE  G P     W                                 RY        G E+        +PPS       +D K+++ +N  ++ E       ++T     +  +  T L+   NG              RY+C A N  G  S  +    L+              ++  +LN    ++ C+A+S     I W K                   + T +E  R++   PG +   L I + + +D   Y+CVA+N+ G    N+EV
Sbjct: 1962 LSRGHVLEIDRAQVSDAGFYKCVAINVAGSTELTYSLQVYV----PPTISSKGGMVTVVVNDP--VRLECEASG-VPVPSLTW-------LKEGSPVSSFSDGIQVLSGGRVLSFISAQVSDAGHYTCVAVNAGGEQHREYEL-RVYVPPNIMGEEVNNTV--LMGQSVQLHCQSDAIPPPALSWRKDGRPLYRKPGLSVSEDGSFLKIISAQVQDTGRYTCEATNVAGKTEKHYNLNVWVPPSIRGSDEVSSLTVIEGGLITLVCESSGIPPPSLI-WKKDGSELKGDSRVRVLSGGRQLQISSAEKADASSYTCLASSAAGSAI---KEYNLQVYVRPSISSSAQDEE-------TVTRGGDITLRCEAD----GVPRPAVSWMKDGRPLSTGRKAQILNEGRLLRILEAQVADTGRYTCIAINVAGQADRKYD--VNVHVPPTIIGQ----------THMPENISVVVKNPVVLTCEASGMPPPAITWLKDGQPISTSSSVRVISGGRGLRLMHAASSDAGRYTCIVSNSAGEERKNFDLYVLVPPSIVNEGTVEDVKVKERQNAILACEVTGNPVPEITWLKDGQPLASDTRLQVMSNGRFLQISASQVADTGRYSCLASNSAGDRSRHFNLNVLVSPTIAGSGPEGSAEEVTVTLNSPTSLV-CEAQSYPPAIITWLK-------------------DGTPFESSRNVRVLPGGRT--LQILNAKEEDAGRYTCVATNEAGETLKNYEV 2649          

HSP 10 Score: 75.8702 bits (185), Expect = 4.078e-13
Identity = 141/619 (22.78%), Postives = 225/619 (36.35%), Query Frame = 3
Query:  537 IAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTI------DLSCEVYANPAATIQW-------VIMQGLQIIDSSKFIAKIRPDMD--GPLICEAKNYLGSTKETVQLNVLRDPLIHIDSNY-RVPKNSNYSINCRVTSNPPPEFIKWYK----LSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKII-SIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETKD-----------IVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNE---LTIKDLENDDFSNYSCVASNDQGIKKVNFEV 2288
            I++QC VSG IPPP  S   +KD   + +          +   V  I   R   LE+ G   C A N AG   ++  L ++   P I+         M+N+TI       L C+   +P   + W           G+ I+     +   R  +   G   C A N  GST+ T  L V   P I        V  N    + C  +  P P  + W K    +S+ +D   VL S    L+FI    S  G Y CV  N              +   +    Y P  P I+G ++  +++ G+   + C +D   P    P L W     KD           +  D    ++I+++ +  G+Y C A N  GK+       V V   PSI    +   + +    L   I+  CE  G P     W+    ++     D ++   R +S  R  QI +    D  S                Y C A +  G A  +Y L +   P I  + +  +         + +RC+A+ +    + W K       P  + +    +A++               +NE   L I + +  D   Y+C+A N  G     ++V
Sbjct: 1836 ISLQCVVSG-IPPP--STTWLKDG--RPVDTTQEFLKLESAGRVLHIKKAR---LEDAGKYTCVATNAAGEAQQHIRL-SVHEPPSIQ-----YAGEMLNETILAGFQIQLKCKATGSPLPAVTWYKDGRPLTSAAGVNILSRGHVLEIDRAQVSDAGFYKCVAINVAGSTELTYSLQVYVPPTISSKGGMVTVVVNDPVRLECEASGVPVPS-LTWLKEGSPVSSFSDGIQVL-SGGRVLSFISAQVSDAGHYTCVAVNA----------GGEQHREYELRVYVP--PNIMGEEVNNTVLMGQSVQLHCQSDAIPP----PALSW----RKDGRPLYRKPGLSVSEDGSFLKIISAQVQDTGRYTCEATNVAGKTEKHYNLNVWV--PPSIRGSDEVSSLTVIEGGL---ITLVCESSGIPPPSLIWKKDGSEL---KGDSRV---RVLSGGRQLQISSAEKADASS----------------YTCLASSAAGSAIKEYNLQVYVRPSISSSAQDEETVTRGGD--ITLRCEADGVPRPAVSWMKDGR----PLSTGR----KAQI---------------LNEGRLLRILEAQVADTGRYTCIAINVAGQADRKYDV 2366          

HSP 11 Score: 75.0998 bits (183), Expect = 6.109e-13
Identity = 134/634 (21.14%), Postives = 237/634 (37.38%), Query Frame = 3
Query:  327 SESQITISHESRLTIHNISSDNSGYYVCRVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQWVIMQGLQIIDSSKFIAKIRPDMDGPL-------------ICEAKNYLG--STKETVQLNVLRDPLIHIDSNY-RVPKNSNYSINC-RVTSNPPPEFIKWYKLSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKW------IDVETKDI-VSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQ---HKNRKVFLSYSDL-PQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVT 2144
              S+  ++ +  L I N    ++G Y C  + +  ++     A+V+    P+    H +  I + Q+     ++C V+G +P P I M+   D  +K   ++S +    T+            Y        C A+N AG   +    L++   P+     P+ ++A I   I L C    +P   + W     + +       + +   MDG L             +CEA+N+ G    K  V +  L  PLI +      V ++   ++ C  +  NP PE    +    +T S  V +  D  L+   + +   G Y C+T+N+           +S +T  L V   P      G +I S ++G    + C    ++ G P P + W      +D+      +++  +  + +     +G++ C A N  G S  +    V V    S + +   H N +  L  S +    I+  CE    P     W    + I P                     F+   I L S +  + T  R + +G Y C A N  G  S    L +  PP I    R  K+      + + + C  + +    + W K  +  VL   S +Y I +  +T
Sbjct:  645 GSSRHRMTPDGTLIIKNTGLKDAGTYGCLASNVAGTDS--QTAIVSYIESPQVTVPHSDLLIGLGQK---TVMECRVTG-VPHPDI-MWYKGDLQLKPSSVLSMDPQRGTLTIQQTQDTHAGQY-------TCVAVNSAGS-AQGYITLDVGSAPQFTKK-PSDLSADIGTNITLPCYAQGHPKPQLSWRREDNIALFTQRHGPSTVTHRMDGGLFITNLWVEDEATYVCEAQNHFGRIQVKAKVTVTGLVSPLIALSPTVVSVIEDQQVTLPCVLLAGNPLPERHWLHDNGLVTSSPYVTVRRDGSLHIEQVSQQDGGQYTCLTENVV---------GSSNRTTILNVYVMPTIQH--GPQIFSTIEGTPISLPC----RAHGVPKPDITWSKRGELLDLSGSVFSLTEDGSLHIHSPSGEESGEFVCTATNAAGYSSRKVQLTVYVRPKLSGVGEADIHDNSEKLLEMSVIVGDDITLPCEVESVPPPIITWAKDKQLISP---------------------FSPRHIQLPSGSM-RITDTRVTDSGMYLCVATNIAGNFSQTVKLSVLVPPSISAGPRAMKVQIG---HAIDLPCVTQGVPEPSVSWLK--DGTVLQDGS-RYRISDGALT 1219          

HSP 12 Score: 67.781 bits (164), Expect = 1.100e-10
Identity = 82/364 (22.53%), Postives = 138/364 (37.91%), Query Frame = 3
Query:  699 LENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQWVIMQGLQIIDS--------SKFIAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLIHIDSNYRVPKNSNYS------INCRVTSNPPPEFIKWYKLSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKW----IDVETKDIVS---DMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWR 1727
            L++ GF  C AI+ AG     T L   +  P I   VP    A     + L+C V +     + W        +D         S  I ++ PD  G   C A N  G +   V LNV   P++ +D     P+N  +       I C     P P  +  +    +  SS   ++ D  L          G Y C+  N+              +T  ++    P+      + +I +  G+K +++C    +  G P P + W    + ++   ++S      T  +  ++  H G+Y C+A N  G +    T  + V  AP    Q   +   LS +D+  +I+  C  +G P  +  WR
Sbjct:  484 LKDEGFYECIAISSAGTGRAQTFLDVSEPPPAIT--VPFNATASPGANVILTCLVTSTVRFNLTWQRGDVDARLDHRIRVTTNLSLEIQRVTPDDVGWYTCIAANEGGVSAARVYLNVQEPPVVTVD-----PRNQTFQTGQEIWIRCSAKGYPSPMVVWTHNDMFIMGSSRHRMTPDGTLIIKNTGLKDAGTYGCLASNVA---------GTDSQTAIVSYIESPQVTVPHSDLLIGL--GQKTVMEC----RVTGVPHPDIMWYKGDLQLKPSSVLSMDPQRGTLTIQQTQDTHAGQYTCVAVNSAGSAQGYIT--LDVGSAP----QFTKKPSDLS-ADIGTNITLPCYAQGHPKPQLSWR 818          

HSP 13 Score: 62.7734 bits (151), Expect = 3.645e-9
Identity = 76/322 (23.60%), Postives = 133/322 (41.30%), Query Frame = 3
Query:  699 LENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPN-TVNAMINQTIDLSCEVYANPAATIQW---------------VIMQGLQI-IDSSKFIAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLI---HIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMTDSS--AVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQIL----GNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWID-------VETKDIVSDMETFQVINSESRHNGKYKCLAENEIGK 1565
            +++ G  +C AIN AG   ++   L++   P I   V +  ++A++NQ I L C+V  +P  TIQW               V+ +G Q+ I S++   + R        C A N  G   +   L++   P I   ++ S   V   +  ++ C V   P P  + WYK   +  SS  A  +     L  +    S  G Y C   ++            +EK + L V Y P  P I     G   + ++  +  I++C  +    G P P+L W+         E+  ++      +++N+     G+Y C+A +  G+
Sbjct: 1415 VQDAGRYICKAINIAGSRERDF-YLDVLVPPTIIGTVGSRDLSAVLNQEIVLECKVKGDPFPTIQWYKDRKPVFLGDPNIEVLNRGQQLKIKSARLGDQAR------YQCSATNAAGKQSKDFNLSIFVPPSIKGGNVSSEVTVLLGNLVTLECEVRGVPLPA-VTWYKNGEVILSSRQAQYVDRGQFLKILRAQVSDAGQYTCRVTSVA---------GTAEKVVELDV-YVP--PSITAGSDGPTDMKVVLNKSLILECEAE----GHPPPSLTWLKDGSPVAVRESLRVLDQGRKIEILNALQSDAGRYVCVATSVAGE 1712          
BLAST of hemicentin-1 isoform X2 vs. Ensembl Zebrafish
Match: hmcn2 (hemicentin 2 [Source:ZFIN;Acc:ZDB-GENE-030131-9765])

HSP 1 Score: 100.138 bits (248), Expect = 1.402e-20
Identity = 117/485 (24.12%), Postives = 186/485 (38.35%), Query Frame = 3
Query:  723 CTAINDAGVLTKNTSL---LNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQWVIMQGLQII-DSSKFIAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLIHIDSNYRVPK--NSNYSINCRVTSNPPPEFIKWYK----LSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRK------PANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETKDIVSDMET-----------FQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIV 2096
            C+A+N AG    N SL   L ++ KP+I P  P+T+ A+I QT+ L C V   P+  + W +  GL +  D    I  ++    G   C A+N  G     + L+VL  P    +    +    NS   I C    +P P+ ++W+K    +        V +++D  L       S +G + CV  N   S   K +      P   +    L +T   K+P  LG     I                   P PT+ W    TKD    +ET            ++   +  H G++ C A+N  G++    T  V   +AP +I+     +     +   Q +   C   G+PL    W +  E + P+  D  +E        R+                    S+R    G Y C A N  G  +  + L I+  P+I+ +    ++   L    V+  C+ E I    I W K N+ IV
Sbjct: 1142 CSAVNPAG----NVSLTYNLQVQAKPRIHP-APSTLKALIGQTVVLPCVVQGEPSPQVSW-LHNGLPVASDRMLKIQAVQHSDSGTYRCVARNSAGEDTIQIVLDVLEGPYFETNGETVIESVANSKVLIPCPARGSPAPQ-VRWFKNGLEVHMERSEHGVSLAEDGALVIGSASASHSGDFKCVATNEAGSVERKTRLKVNVPPEIQDDGQLLNLTVTLKQPLTLGCDAFGI-------------------PTPTISW----TKDNRPVLETPGVYLQNGKRLLKIYRVQHDHAGRFSCTAQNSAGEAKREYTIEV---QAPPVISGTSGVQEVTIMAG--QEVDMQCRVSGRPLPSVEWTHDGEVLSPNG-DPHVEFLEKGQVLRVK-------------------SVRPRDRGLYQCVATNNAGTQTRQFRLTIQAAPLIRDSGEDSEVTVVLGFPAVL-HCEVEGIPLPTITWLKDNQPIV 1570          

HSP 2 Score: 83.1889 bits (204), Expect = 2.405e-15
Identity = 106/433 (24.48%), Postives = 178/433 (41.11%), Query Frame = 3
Query:  711 GFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQWVIMQGLQIIDSSKF---------IAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLIHIDS-NYRVPKNSNYSINCRVTSNPPPEFIKWYKLSN-MTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQI-LGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETKDI-VSDM-------ETFQVI--NSESRHNGKYKCLAENEIGKSFSRETALVTVN------EAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGI 1925
            G   C+A N  GV  K+ +L  ++  P+I+P +   V  +++ ++ L CEV   P  TI W   +G+ +    +           +++     G   C A+N  G+     QL +   P++ +    Y V      S++C     P PE + W++    + DSS + +  +  L+     R   G+Y C  +N    + G+   A+ +  + L   + P  PQI +G   IS+M+G + ++ C+    + G P P + W   E K + VS+M       E+  +I   +E+   G + C+A N  G   +R    +TVN      E P  +T +K            QS+S +C   G P     W   N     +S D            R S IF  +T+               S  G Y C A+N  G+
Sbjct: 2922 GKYTCSARNPVGVAYKHVTL-TVQEPPEIRP-MAEEVQVVLHHSVVLPCEVQGFPRPTITWQ-REGVPVATGHRLAVLSNGALKFSRVTLGDAGTYQCLAQNEAGTALAQTQLILQVPPVLSVPRLEYVVVLGQPVSLDCVADGQPKPE-VTWHRDRRPVVDSSHLRLFSNGSLHITATQRGDAGIYTCSARN----SVGR---ASHDVRLIL---HTP--PQIFVGQSEISVMQGLRVMLPCA----AQGVPEPRMSW---EKKGVSVSNMPGKFTLRESGGLIIERAEAGDAGLFTCVATNTAGS--ARWDIRLTVNMRPAFKELPGDVTLNKG-----------QSLSLSCHAEGTPTPTITWTANNRPYTGASVD---------ETGRSSVIFDNVTV---------------SDGGTYACIAENTVGL 3294          

HSP 3 Score: 81.6481 bits (200), Expect = 5.776e-15
Identity = 146/698 (20.92%), Postives = 255/698 (36.53%), Query Frame = 3
Query:  315 VVPDSESQITISHE-SRLTIHNISSDNSGYYVCRVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQE-FIAIQCSVSGGIPPPKISMYIVKDN----AIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMIN-QTIDLSCEVYANPAATIQW------VIMQGLQIIDSSK---FIAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLIH---IDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSN-MTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGN---KIISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETK-------DIVSDMETFQVINSESRHNGKYKCLAENEIGKS---FSRETALVTV-NEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQP 2306
            +V  +   I IS + S LT  ++  ++SG Y C        E  I      V   P SIS   +    V   Q   + ++C   G  P P+IS   ++D        R++++S++   ST+     IS   P +L + G   C A + AG+   N  L  ++  P ++   P    A++   ++ L+CE    P  T+ W      + +    ++D  +    ++ + P   G   C A N  GS+ +T  L VL  P I          +  +S   + C  T  PPP  + W K    + D+ A++  D   L    +     G+Y C+  +          PA  +          P  P +LG+   + +S+       ++C TD   P    P ++W     K         ++  +  ++ +     +G+Y C+  N  G +   F+ E  L  V  E  S+I  H           + Q     CE  G P     WR     +P             + N R +         L S    + ++++ S  GRY C   N+ G  S    + +K       +     +     Q  V+  C++  I    + W +    I +  QS  Y +  +                    L I     +D   + C  +N+ G ++   EV+ +  P
Sbjct: 2248 LVNGNTEHINISPDGSTLTFLSVKPEDSGTYTCLAVSSAGQETKIYTLFALV---PPSISGETSVPREVHTTQHSVVTLECKAKG-TPQPQISW--LRDGHPLLLSPRIRLLSAD---STLR----IS---PVHLSDSGIYTCVARSRAGLAELNFDL-QVQAPPAVERTEPTEQVAVVQGSSVTLTCEARGVPPPTLSWLKDGQPLSLHRNLLLDGQETRFLLSDVGPSDAGLYSCVASNQAGSSTKTFNLTVLEPPKITGSLSPEEQLIAVDSLLELECIATGLPPPT-LSWLKDGRPIQDNMAIIERDGQLLRINKVQVEDAGLYTCLASS----------PAGEDGRNHWVRVQLP--PTLLGSNDIRTVSVPAKGHLTLECQTDSDPP----PEIEWYKDNVKVQLGGRIQSIASGQFLEIQDVRMHDSGQYSCVVTNIAGSTSLFFTVEILLPPVIREGSSLIIAH-----------VGQDAVLPCEVEGAPSSSVMWRKDGGPVP-------------LHNNRFT---------LLSEGSLRISTVQLSDVGRYYCSVSNQAG--SDHRGMDLKVYVGPSISPGPFNVTVVTGQRAVL-SCESTGIPAPQVSWRRNGSPISVD-QSSAYRLMSS------------------GSLVITSPTAEDEGYFECTVTNEVGEERRVIEVILQVPP 2856          

HSP 4 Score: 81.2629 bits (199), Expect = 8.159e-15
Identity = 119/555 (21.44%), Postives = 211/555 (38.02%), Query Frame = 3
Query:  723 CTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQWV-------IMQGLQIIDSSKFIAKIRPDMD--GPLICEAKNYLGSTKETVQLNVLRDPLI----HIDSNYRVP-KNSNYSINCRVTSNPPPEFIKWYKLSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETKDIVSDMETFQVINSESR------------HNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARW-RYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQP 2306
            C A N AG + +  SL +I+  P+I       ++ +    I L C+V A P A I W           G+ I+   + +   R      G  +C A N  G  ++++ L +   P +      +S  R P   S   + C     P PE + WY+      +   L  +  +L  + +  +  G+Y C   NI             ++T  +TV   P     L   +   + G    + C    ++ G P P + W+    KD  S +E+    N  +R            H G Y C+A+N  G++  ++   +T+   PSII      +V    + + + ++  C   G P  +  W R G   +  +++      H NIS +  +  F                S++   +G Y C A +  G  +  Y L    PP I       +   +   +VV + C+A+      I W +    ++L   S +  +  A+ T                 L I  +   D   Y+CVA +  G+ ++NF++  ++ P
Sbjct: 1908 CVAENKAGAVERLYSL-SIQVPPRIVGRREEEMSVVEGHMISLLCDVQAYPPADIIWTKDGQMLEFTTGIHILPGGQMLQIPRAGQQDAGQYVCTATNSAGQDQKSILLTIYALPALVPRLESESEVRTPLVGSTLILRCEANGIPKPE-VTWYRNGLQLAAGNGLRIERQQLEIVGVQVADGGVYTCKVSNIA---------GQVDRTFRVTVHVPPVMEGPLHETLTQNL-GSHITLIC----EASGIPVPNIAWL----KD-GSPIESSLQWNWSTRANRLELGPLQLSHAGTYTCIAKNTEGQT--QKDYSLTIYAPPSIIDSGHPSEV---SAPVGEELALECRAMGSPAPKVSWLRNGKTLVNGNTE------HINISPDGSTLTFL---------------SVKPEDSGTYTCLAVSSAGQETKIYTLFALVPPSISGETSVPREVHTTQHSVVTLECKAKGTPQPQISWLRDGHPLLL---SPRIRLLSADST-----------------LRISPVHLSDSGIYTCVARSRAGLAELNFDLQVQAPP 2395          

HSP 5 Score: 73.559 bits (179), Expect = 2.014e-12
Identity = 106/523 (20.27%), Postives = 198/523 (37.86%), Query Frame = 3
Query:  828 MINQTIDLSCEVYANPAATIQWVIMQGLQIIDSSKF----------IAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLIHIDSNYRVPK-----NSNYSINCRVTSNPPPEFIKWYK--LSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNK--IISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETKD-----------IVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQP 2306
            ++  +I LSCE  A P   + W   QG Q+  S             I +++ +  G   C+A N  G  K    L VL  P+I   ++  +       NS  S+ C V+ +P P  + W K  L   +DS   ++ D   L  + +  S    Y CV +N         K    E+   L++   P+   I+G +   +S+++G    + C      P +    + W    TKD           I+   +  Q+  +  +  G+Y C A N  G+   +++ L+T+   P+++ + ++    +    +  ++   CE  G P  E  W Y N     +    +IE                       R   +   ++ +  G Y C+  N  G     + + +  PP+++  + H  +  +L  ++ +I C+A  I   +I W K    I    Q                    +    + N L +  L+      Y+C+A N +G  + ++ +   + P
Sbjct: 1755 LLGHSISLSCESNAIPPPRLSWY-RQGRQLTSSDGVVLAPGGHILQIPRVQLEDAGKYTCQAVNEAGEDKMHYDLEVLVPPVISGQTDEFMQDVDTVVNSTVSLRCDVSGHPSPS-VSWIKDGLPLYSDSRHNILEDGKLLEIVNVQVSDGASYQCVAEN---------KAGAVERLYSLSIQVPPR---IVGRREEEMSVVEGHMISLLCDVQAYPPAD----IIW----TKDGQMLEFTTGIHILPGGQMLQIPRAGQQDAGQYVCTATNSAGQ--DQKSILLTIYALPALVPRLESESE-VRTPLVGSTLILRCEANGIPKPEVTW-YRNGLQLAAGNGLRIE-----------------------RQQLEIVGVQVADGGVYTCKVSNIAGQVDRTFRVTVHVPPVMEGPL-HETLTQNLGSHITLI-CEASGIPVPNIAWLKDGSPIESSLQ--------------------WNWSTRANRLELGPLQLSHAGTYTCIAKNTEGQTQKDYSLTIYAPP 2206          

HSP 6 Score: 70.4774 bits (171), Expect = 1.626e-11
Identity = 145/674 (21.51%), Postives = 231/674 (34.27%), Query Frame = 3
Query:  336 QITISHESRLTIHNISSDNSGYYVCRVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQWVIMQGLQIIDSSKF-------------IAKIRPDMDGPLICEAKNYLGSTKETVQLNV--LRDPLI-----HIDSNYRVPKNSNYSINCRVTSNPP-PEFIKWYKLSNMTDSSAVLISDDAKLNFILIDRS---STGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWI---DVETKDIVSDME----TFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQG 2264
            +++IS    LTI   + ++SG Y C   L T      S +V      P SIS      + V  E    +++C  SG   P  I  +   D  +        +    T+       L       + G   C A N AG  +    +L +   P      P  + A +   I L C     P  ++ W    G  I   S               I  +  D +G  +CEAKN  GS K   ++ V  L  PL+      I +    P N    + CR+    P PE I  +    +     V +  D  L+   +DR+     G Y C   N+  S               +TVT +   P  +    +     E   I  S + +  G P PT+ W    D   + + +  E    +  + +      G Y C A + +G + SRE  L+ VN  P I+    ++      +++   +   CE +G P     W    + IPP +  F +    ++    +  + +KL                      Y C A N  G  SL Y L  +     + +     + A + Q VV+  C  +   +  + W     H  LP  S +                          L I+ +++ D   Y CVA N  G
Sbjct:  652 RMSISESGMLTIRGAAPEDSGNYTC---LATNEVGTASQSVNVNYAEPPSISVVQQMVMVVAGEDA--SLECQASG--TPSPIVKWQKGDLDLGTAPFAEQDVHRGTLQIRGVQEL-------DSGDYTCVASNIAGS-SSAVVVLQVGAAPTFSE-SPVDITASVGDNITLPCIARGFPTPSLIWRRQDGRPIFGKSSGHVGTSQLPSGALQIQSVWVDDEGVYLCEAKNQFGSIKALARVTVTGLEPPLLVQGAPSITTMIAQPLN----LPCRLLDGIPLPERIWTHNGKQVRVGGRVFLRSDGSLH---VDRAVPEDAGTYVCTAVNVAGSAN-------------ITVTVEVHAPPDIKAGPLHYTASEGTPISLSCEAR--GVPKPTISWSKGRDPLNRPVSAPFEAADGSLYISSPTEEDAGVYICTATSAVGYT-SREMHLI-VNSKPRIVGADGSQVTVKMAAEVGSEVILPCEVQGSPTPLVTWSRNGQTIPPVTAWFTMLPSGSLKISDVRVVDSKL----------------------YTCSAVNPAGNVSLTYNL--QVQAKPRIHPAPSTLKALIGQTVVL-PCVVQGEPSPQVSWL----HNGLPVASDRM-------------------------LKIQAVQHSDSGTYRCVARNSAG 1231          

HSP 7 Score: 68.5514 bits (166), Expect = 6.201e-11
Identity = 114/548 (20.80%), Postives = 206/548 (37.59%), Query Frame = 3
Query:  537 IAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIK----PFVPNTVNAMINQTIDLSCEVYANPAATIQWVIMQGLQIIDSSKF----------IAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLI--HIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMTD--SSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPK-EPQILG-NKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWID-----VETKDIVSDMETFQVINSESRHNGKYKCLAEN---EIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIV 2096
            I++ C  S  IPPP++S Y           ++ +  P   I ++  + L      E+ G   C A+N+AG   K    L +   P I      F+ + V+ ++N T+ L C+V  +P+ ++ W I  GL +   S+           I  ++        C A+N  G+ +    L++   P I    +    V +    S+ C V + PP + I W K   M +  +   ++     L      +   G Y C   N              +K++ LT+   P   P++   +++ + + G   I++C  +    G P P + W            +  + +  +++  +    G Y C   N   ++ ++F     +  V E P   T  +N         L   I+  CE  G P+    W      I  S Q        N S  R +++              +   L+ S  G Y C A N  G    DY L I  PP I  +    +++A + + + +  C+A       + W +  + +V
Sbjct: 1760 ISLSCE-SNAIPPPRLSWYRQGRQLTSSDGVVLA--PGGHILQIPRVQL------EDAGKYTCQAVNEAGE-DKMHYDLEVLVPPVISGQTDEFMQD-VDTVVNSTVSLRCDVSGHPSPSVSW-IKDGLPLYSDSRHNILEDGKLLEIVNVQVSDGASYQCVAENKAGAVERLYSLSIQVPPRIVGRREEEMSVVEGHMISLLCDVQAYPPADII-WTKDGQMLEFTTGIHILPGGQMLQIPRAGQQDAGQYVCTATN---------SAGQDQKSILLTIYALPALVPRLESESEVRTPLVGSTLILRCEAN----GIPKPEVTWYRNGLQLAAGNGLRIERQQLEIVGVQVADGGVYTCKVSNIAGQVDRTFRVTVHVPPVMEGPLHETLTQN---------LGSHITLICEASGIPVPNIAWLKDGSPIESSLQ-------WNWST-RANRL--------------ELGPLQLSHAGTYTCIAKNTEGQTQKDYSLTIYAPPSIIDSGHPSEVSAPVGEELAL-ECRAMGSPAPKVSWLRNGKTLV 2249          

HSP 8 Score: 61.6178 bits (148), Expect = 6.976e-9
Identity = 112/519 (21.58%), Postives = 185/519 (35.65%), Query Frame = 3
Query:  363 LTIHNISSDNSGYYVCRVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMY----IVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNT-VNAMINQTIDLSCEVYANPAATIQWV-----IMQGLQII----DSSKFIAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLIHIDSNY--------RVPKNSNYSINCRVTSNPPPEFIKWYK---------LSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQN------------IQIS-------------TTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETKDIVSD-------METFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSD--LPQSISFTCEFRGQPLYEARW 1724
            L I+ +  D++G + C  T   ++        + V+ PP        + + +   QE + +QC VSG  P P +       ++  N    ++ +          +V  +   RP    +RG   C A N+AG  T+    L I+  P I+    ++ V  ++     L CEV   P  TI W+     I+   Q+       S  +A  R +  G   C A N  G+      L ++  P I  DS          +V  N   +++C     P P   +W+K          S ++ +S +L  D+A L       S  G Y CV  N            IQ+              T G+R+    +          P  P  +  +   ++ G    + C ++   P    P L W     +   SD           Q+   +    GKY C A NE G+        V V   P +I+   +   F+   D  +  ++S  C+  G P     W
Sbjct: 1396 LKIYRVQHDHAGRFSC--TAQNSAGEAKREYTIEVQAPPVISGTSGVQEVTIMAGQE-VDMQCRVSGR-PLPSVEWTHDGEVLSPNGDPHVEFLEK-------GQVLRVKSVRP---RDRGLYQCVATNNAGTQTRQFR-LTIQAAPLIRDSGEDSEVTVVLGFPAVLHCEVEGIPLPTITWLKDNQPIVSSPQLTYTQGGQSLRVAAARGEDAGAYTCRATNPAGTAHRHYTLRIMVPPQIEGDSTILSFGRREEKVRINGTLTLSCLAKGFPEP-VTQWFKDGQLLNGNTHSGLSVNSHMLHVDNAML-------SHEGQYTCVVSNSAGEDKRDFHLTIQVPPVFHRVINGAADFTLGEREGDEED----------PDSPDGMVER-REVLLGHSISLSCESNAIPP----PRLSWYRQGRQLTSSDGVVLAPGGHILQIPRVQLEDAGKYTCQAVNEAGEDKMHYDLEVLV---PPVISGQTDE--FMQDVDTVVNSTVSLRCDVSGHPSPSVSW 1871          

HSP 9 Score: 60.8474 bits (146), Expect = 1.206e-8
Identity = 111/547 (20.29%), Postives = 203/547 (37.11%), Query Frame = 3
Query:  363 LTIHNISSDNSGYYVCRVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSL---LNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQWVIMQGLQIIDSSKF---------IAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLIHIDS-NYRVPKNSNYSINCRVTSNPPPEFIKWYKLSN---MTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISI--MKGEKFIIKCSTDRKSPGEPFPTL----KWIDVETKD---IVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIA 1928
            L I+ +  +++G Y C  +     +       V V+ PP  +  +  ++++V   +  + ++C      PPP+I  Y  KDN   +L     +  +    E+  + +       + G   C   N AG    +TSL   + I   P I+    + + A + Q   L CEV   P++++ W    G   + +++F         I+ ++    G   C   N  GS    + L V   P I     N  V       ++C  T  P P+ + W +  +   +  SSA  +     L          G + C   N              E+ +   +    + P ++ + + S+  +K    ++ C     + G P P +     W+ +  +     V      +++ +   H GKY C A N +G ++   T  +TV E P I    +  +V L +     S+   CE +G P     W+   E +P ++       HR       +  F+++T+                  G Y C A N  G A
Sbjct: 2544 LRINKVQVEDAGLYTCLASSPAGEDG--RNHWVRVQLPPTLLGSNDIRTVSV-PAKGHLTLECQTDSD-PPPEIEWY--KDNVKVQLGGRIQSIASGQFLEIQDVRM------HDSGQYSCVVTNIAG----STSLFFTVEILLPPVIREG-SSLIIAHVGQDAVLPCEVEGAPSSSVMWRKDGGPVPLHNNRFTLLSEGSLRISTVQLSDVGRYYCSVSNQAGSDHRGMDLKVYVGPSISPGPFNVTVVTGQRAVLSCESTGIPAPQ-VSWRRNGSPISVDQSSAYRLMSSGSLVITSPTAEDEGYFECTVTN----------EVGEERRVIEVIL---QVPPVIKDDVSSVTAVKMSPVVLPC----HATGRPEPVISWNKNWMQLGARGGSYRVLPTGALEILAATPSHAGKYTCSARNPVGVAYKHVT--LTVQEPPEIRPMAEEVQVVLHH-----SVVLPCEVQGFPRPTITWQ--REGVPVAT------GHRLAVLSNGALKFSRVTL---------------GDAGTYQCLAQNEAGTA 3025          

HSP 10 Score: 60.4622 bits (145), Expect = 1.916e-8
Identity = 98/448 (21.88%), Postives = 168/448 (37.50%), Query Frame = 3
Query:  663 EVSAISLWRPHYL--ENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQWVIMQGLQ---------IIDSSKFIAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLIHIDSNYRVPKNSNYS------INCRVTSNPPPEFIKWYKLSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKW----IDVETKDIVSD---METFQVINSESRHNGKYKCLAENEIGKSFS----RETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHG 1922
            E S  + W   Y   ++ G   C A + AG+    T L   +  P ++P  P  V +       LSC++  +    + W                + +SS  I+ +R    GP  C A N  G ++  V L VL  P + +      P+  ++S      I+C  + +PPP+    +    +++   + IS+   L         +G Y C+  N ++ T  +           + V Y       +  +++ ++ GE   ++C    ++ G P P +KW    +D+ T           T Q+   +   +G Y C+A N  G S +    +  A  T +E+P  IT           + +  +I+  C  RG P     WR               +D R I  +    + T     L S     Q S+     G Y CEA N+ G
Sbjct:  473 EASGKASWEIPYASGDDEGAYECVARSTAGMGRALTQLTVREPPPVLRP--PVNVTSSPGTVAVLSCQIQGSVRHNLTWYRTGSALPSRSGRVRVLANSSLEISWVRSQDAGPYQCRASNAHGESQAVVWLVVLEAPSVVVS-----PQTQSFSLGAEVHISCSASGSPPPKVFWSHGNIFLSNHHRMSISESGMLTIRGAAPEDSGNYTCLATN-EVGTASQS----------VNVNYAEPPSISVVQQMVMVVAGEDASLEC----QASGTPSPIVKWQKGDLDLGTAPFAEQDVHRGTLQIRGVQELDSGDYTCVASNIAGSSSAVVVLQVGAAPTFSESPVDIT-----------ASVGDNITLPCIARGFPTPSLIWRR--------------QDGRPIFGKSSGHVGTS---QLPSGALQIQ-SVWVDDEGVYLCEAKNQFG 869          

HSP 11 Score: 55.4546 bits (132), Expect = 6.090e-7
Identity = 100/445 (22.47%), Postives = 166/445 (37.30%), Query Frame = 3
Query:  318 VPDSESQITISHESRLTIHNISSDNSGYYVCRVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISM------YIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQW--VIMQ------GLQIIDSSKF-IAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLIH-IDSNYRVPKNSNYSINCRVTSNPPPEFIKWYK--LSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIIS--IMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETKDIVSDMETFQVINSESRH--------NGKYKCLAENEIGKS 1568
            VP   ++ T+  E  L I  +   + G Y C V+    S+H      + V   P      FN ++   Q     A+    S GIP P++S         V  ++  RL    S    S  A             E+ G+  CT  N+ G   +   ++ ++  P IK  V ++V A+    + L C     P   I W    MQ        +++ +    I    P   G   C A+N +G   + V L V   P I  +    +V  + +  + C V   P P  I W +  +   T     ++S+ A L F  +     G Y C+ QN       +   A ++  + L V      P +L    +   ++ G+   + C  D    G+P P + W     +  V D    ++ ++ S H         G Y C A N +G++
Sbjct: 2713 VPLHNNRFTLLSEGSLRISTVQLSDVGRYYCSVSNQAGSDH--RGMDLKVYVGPSISPGPFNVTVVTGQR----AVLSCESTGIPAPQVSWRRNGSPISVDQSSAYRLMSSGSLVITSPTA-------------EDEGYFECTVTNEVGEERRVIEVI-LQVPPVIKDDV-SSVTAVKMSPVVLPCHATGRPEPVISWNKNWMQLGARGGSYRVLPTGALEILAATPSHAGKYTCSARNPVGVAYKHVTLTVQEPPEIRPMAEEVQVVLHHSVVLPCEVQGFPRPT-ITWQREGVPVATGHRLAVLSNGA-LKFSRVTLGDAGTYQCLAQN-------EAGTALAQTQLILQV------PPVLSVPRLEYVVVLGQPVSLDCVAD----GQPKPEVTW--HRDRRPVVDSSHLRLFSNGSLHITATQRGDAGIYTCSARNSVGRA 3115          
BLAST of hemicentin-1 isoform X2 vs. Ensembl Zebrafish
Match: robo2 (roundabout, axon guidance receptor, homolog 2 (Drosophila) [Source:ZFIN;Acc:ZDB-GENE-001019-1])

HSP 1 Score: 91.6633 bits (226), Expect = 4.444e-18
Identity = 130/541 (24.03%), Postives = 205/541 (37.89%), Query Frame = 3
Query: 1020 LNVLRDPLIHIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMTDSSAVLIS-DDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFI-IKCSTDRKSPGEPFPTLKWI--DVETKDIVSDME------TFQVINSESRHNGKYKCLAENEIGKSFSRETALVTV--NEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERIS-QIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHG--IASLDYFLXXXXXXXXXXNIRHRKIAASLN-QNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQG---------IKKVNFEVVFKSQ-----PDPPYDLRVTDFEWDSVTINWKPGFNGGLT-QNYEILVISENFGTQNVPFYSDKNENTLTITGLYPSTPYEIRIYAVNSIGRS 2549
            ++VLRD      ++  V       + C      P   I W K     D     I     KL      +S  GMY CV  N+     G+R    ++ T+F       + P  L   I  ++  E+ +  +C       G+P P+++W   DVE      DM+      T ++  + +   G + C+AEN +GK  +  T  V V  + AP  + + +++ V        ++ SF CE +G P     W+         SQ+    +     N R S      LTI          T+++ +  G Y C+A    G  +A     +           IR      +L   +V ++RC A       I W K  + + L  +  + S+           +DL         L IK L   D   Y+CVA++  G         +K+    VV K +     P PP   +VTD   +SV+++W+PG  G      Y I   S++                 TI GL P+T Y   + AVN  G S
Sbjct:    1 VSVLRDDFRQNPTDVVVAAGEPAILECVPPRGHPEPTIYWKKDKVRIDEKDDRIKIRGGKLMISNTRKSDAGMYICVGTNM----VGERDSETAQVTVF-------ERPTFLRRPINQVVLEEEAVEFRCQVQ----GDPQPSIRWKKDDVEIPRGRYDMKFDKDDFTLRIKKATASDEGTFTCVAENRVGKMEASATLTVRVLGSAAPQFVIRPRDQIVAQG-----RTASFPCETKGNPQPAVFWQK------EGSQNLLFPNQPQQPNSRFSVSPSGDLTI----------TTVQRADAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIIRQGPANQTLGVDSVALLRCHASGEPVPTISWLK--DGVSLLGKDPRMSL-----------QDL-------GSLQIKGLRLSDSGIYTCVAASSSGETSWSAFLEVKEAGSLVVVKDRDENELPGPPSKPQVTDVTKNSVSLSWQPGLPGASAISTYVIEAFSQSVSNSWQTVADHAKTTQYTIKGLRPNTIYLFMVRAVNVQGLS 485          

HSP 2 Score: 55.8398 bits (133), Expect = 4.305e-7
Identity = 75/342 (21.93%), Postives = 129/342 (37.72%), Query Frame = 3
Query:  705 NRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMI--NQTIDLSCEVYANPAATIQWV-----IMQGL--QIIDSSKFIAKIRPDM---DGPLICEAKNYLGSTKETVQLNVL----RDPLIHIDSNYR-VPKNSNYSINCRVTSNPPPEFIKWYKLSNMT---------DSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETKDIVS-----------DMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSII 1619
            + G  +C   N  G     T+ + +  +P    F+   +N ++   + ++  C+V  +P  +I+W      I +G      D   F  +I+      +G   C A+N +G  + +  L V       P   I    + V +    S  C    NP P    W K  +            +S   +S    L    + R+  G Y C    +  S   K   A  E T  LT    P   Q   N+ + +      +++C     + GEP PT+ W+    KD VS           D+ + Q+       +G Y C+A +  G+  +  +A + V EA S++
Sbjct:   70 DAGMYICVGTNMVGERDSETAQVTVFERPT---FLRRPINQVVLEEEAVEFRCQVQGDPQPSIRWKKDDVEIPRGRYDMKFDKDDFTLRIKKATASDEGTFTCVAENRVGKMEASATLTVRVLGSAAPQFVIRPRDQIVAQGRTASFPCETKGNPQPAVF-WQKEGSQNLLFPNQPQQPNSRFSVSPSGDLTITTVQRADAGYYICQALTVAGSILAK---AQLEVTDVLTDRPPPIIRQGPANQTLGV--DSVALLRC----HASGEPVPTISWL----KDGVSLLGKDPRMSLQDLGSLQIKGLRLSDSGIYTCVAASSSGE--TSWSAFLEVKEAGSLV 392          
BLAST of hemicentin-1 isoform X2 vs. Ensembl Zebrafish
Match: robo2 (roundabout, axon guidance receptor, homolog 2 (Drosophila) [Source:ZFIN;Acc:ZDB-GENE-001019-1])

HSP 1 Score: 91.6633 bits (226), Expect = 4.444e-18
Identity = 130/541 (24.03%), Postives = 205/541 (37.89%), Query Frame = 3
Query: 1020 LNVLRDPLIHIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMTDSSAVLIS-DDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFI-IKCSTDRKSPGEPFPTLKWI--DVETKDIVSDME------TFQVINSESRHNGKYKCLAENEIGKSFSRETALVTV--NEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERIS-QIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHG--IASLDYFLXXXXXXXXXXNIRHRKIAASLN-QNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQG---------IKKVNFEVVFKSQ-----PDPPYDLRVTDFEWDSVTINWKPGFNGGLT-QNYEILVISENFGTQNVPFYSDKNENTLTITGLYPSTPYEIRIYAVNSIGRS 2549
            ++VLRD      ++  V       + C      P   I W K     D     I     KL      +S  GMY CV  N+     G+R    ++ T+F       + P  L   I  ++  E+ +  +C       G+P P+++W   DVE      DM+      T ++  + +   G + C+AEN +GK  +  T  V V  + AP  + + +++ V        ++ SF CE +G P     W+         SQ+    +     N R S      LTI          T+++ +  G Y C+A    G  +A     +           IR      +L   +V ++RC A       I W K  + + L  +  + S+           +DL         L IK L   D   Y+CVA++  G         +K+    VV K +     P PP   +VTD   +SV+++W+PG  G      Y I   S++                 TI GL P+T Y   + AVN  G S
Sbjct:    1 VSVLRDDFRQNPTDVVVAAGEPAILECVPPRGHPEPTIYWKKDKVRIDEKDDRIKIRGGKLMISNTRKSDAGMYICVGTNM----VGERDSETAQVTVF-------ERPTFLRRPINQVVLEEEAVEFRCQVQ----GDPQPSIRWKKDDVEIPRGRYDMKFDKDDFTLRIKKATASDEGTFTCVAENRVGKMEASATLTVRVLGSAAPQFVIRPRDQIVAQG-----RTASFPCETKGNPQPAVFWQK------EGSQNLLFPNQPQQPNSRFSVSPSGDLTI----------TTVQRADAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIIRQGPANQTLGVDSVALLRCHASGEPVPTISWLK--DGVSLLGKDPRMSL-----------QDL-------GSLQIKGLRLSDSGIYTCVAASSSGETSWSAFLEVKEAGSLVVVKDRDENELPGPPSKPQVTDVTKNSVSLSWQPGLPGASAISTYVIEAFSQSVSNSWQTVADHAKTTQYTIKGLRPNTIYLFMVRAVNVQGLS 485          

HSP 2 Score: 55.8398 bits (133), Expect = 4.305e-7
Identity = 75/342 (21.93%), Postives = 129/342 (37.72%), Query Frame = 3
Query:  705 NRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMI--NQTIDLSCEVYANPAATIQWV-----IMQGL--QIIDSSKFIAKIRPDM---DGPLICEAKNYLGSTKETVQLNVL----RDPLIHIDSNYR-VPKNSNYSINCRVTSNPPPEFIKWYKLSNMT---------DSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETKDIVS-----------DMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSII 1619
            + G  +C   N  G     T+ + +  +P    F+   +N ++   + ++  C+V  +P  +I+W      I +G      D   F  +I+      +G   C A+N +G  + +  L V       P   I    + V +    S  C    NP P    W K  +            +S   +S    L    + R+  G Y C    +  S   K   A  E T  LT    P   Q   N+ + +      +++C     + GEP PT+ W+    KD VS           D+ + Q+       +G Y C+A +  G+  +  +A + V EA S++
Sbjct:   70 DAGMYICVGTNMVGERDSETAQVTVFERPT---FLRRPINQVVLEEEAVEFRCQVQGDPQPSIRWKKDDVEIPRGRYDMKFDKDDFTLRIKKATASDEGTFTCVAENRVGKMEASATLTVRVLGSAAPQFVIRPRDQIVAQGRTASFPCETKGNPQPAVF-WQKEGSQNLLFPNQPQQPNSRFSVSPSGDLTITTVQRADAGYYICQALTVAGSILAK---AQLEVTDVLTDRPPPIIRQGPANQTLGV--DSVALLRC----HASGEPVPTISWL----KDGVSLLGKDPRMSLQDLGSLQIKGLRLSDSGIYTCVAASSSGE--TSWSAFLEVKEAGSLV 392          
BLAST of hemicentin-1 isoform X2 vs. Ensembl Xenopus
Match: lrrc8c (leucine rich repeat containing 8 VRAC subunit C [Source:Xenbase;Acc:XB-GENE-5797483])

HSP 1 Score: 111.694 bits (278), Expect = 5.254e-24
Identity = 153/671 (22.80%), Postives = 268/671 (39.94%), Query Frame = 3
Query:  714 FVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQWVIMQGLQIIDSSK----------------FI-----AKIRPDMDGPLICEAKNYLG---STKETVQLNVLRDPLIHIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNM--TDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVE-----TKDIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARW-RYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNV-----VMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQ-----------------PDPPYDLRVTDFEWDSVTINWKPGFNGGLT-QNYEILVISENFGTQNVPFYSDKNENTLTITGLYPSTPYEIRIYAVNSIGRSLNSE 2561
            F++C  +     LT  + L    + P+I    P+ +     +   L+C+    P  TI+W   +G   +++ K                F+      KIRPD +G  +C A+NYLG   S   ++++ +LRD      S+  V       + C+     P   I W K       D    +     KL      +S  G Y CV  N+     G+R+   +E    LTV  +P   +   N  +++    +F  KC    ++ G+P PT++W   +     ++  + D  T ++    +   G Y C+AEN +GK  +  +A++TV E P+ + + +++ V L      ++++F CE  G P     W + G++ +      F  +  ++ S   +SQ    LTI          T+++ S  G Y C+  N  G  +   FL +      +     R+    +NQ V     +++ C A  I    I W K  + I++P Q  +    ++                    L I+  +  D   Y+C AS   G  + N+    + Q                 P  P    VTD   ++VT++W+P  N G T  +Y I   S   G+       +   ++  +  L P+  Y   + AVN+ G S  S+
Sbjct:    9 FLLCGIM----CLTSGSRLRQEDFPPRIVEH-PSDLIVSKGEPATLNCKAEGRPTPTIEW--YKGGDKVETDKDDPRSHRMLLPSGSLFFLRIVHGRKIRPD-EGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGTPIDDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNM----VGERESEVAE----LTVLERPSFVRRPSNMAVTVDDSVEF--KC----EARGDPVPTVRWRKDDGDLPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGK--AEASAVLTVQEPPNFVVKPRDQVVALG-----RTVTFQCEATGNPQPAIFWQKEGSQNL-----LFSYQPPQSSSRFSVSQT-GDLTI----------TTVQKSDVGYYICQTLNVAGSITTKAFLEVTDVVADRPPPVIRQ--GPVNQTVAVDGTLIMNCIASGIPVPTIHWRK--DGILIPTQDSRIKQQDS------------------GALQIRYAKLSDTGRYTCTASTTSG--EANWSAYIEVQEFGVPVQPPRATDPNLIPSAPSKPEVTDVSKNTVTLSWQPNLNSGATPTSYLIEAFSHASGSSWQTVAENVKTDSFAVKALKPNAIYLFLVRAVNAYGLSDPSQ 610          

HSP 2 Score: 63.929 bits (154), Expect = 1.808e-9
Identity = 96/429 (22.38%), Postives = 152/429 (35.43%), Query Frame = 3
Query:  390 NSGYYVC--RVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISM----YIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFV--PNTVNAMINQTIDLSCEVYANPAATIQWVIMQG------LQIIDSSKF-IAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDP-LIHIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMT---------DSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVT--YKPKEPQILGNKII--SIMKGEKFIIKCSTDRKSPGEPFPTLKW----IDVETKDI---VSDMETFQVINSESRHNGKYKCLAENEIGKS 1568
            + G YVC  R  L     H  SL V  ++       F  N S  +    E   ++C    G P P IS       + D+  +R+ I               I+  R     + G  +C   N  G      + L +  +P    FV  P+ +   ++ +++  CE   +P  T++W    G       +I D     I K+     G   C A+N +G  + +  L V   P  +    +  V      +  C  T NP P  I W K  +            SS   +S    L    + +S  G Y C T N+  S T K          FL VT     + P ++    +  ++      I+ C       G P PT+ W    I + T+D      D    Q+  ++    G+Y C A    G++
Sbjct:  103 DEGVYVCVARNYLGEAVSHNASLEVAILRD-----DFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGTPIDDDKDERITIRGGKL---------MITYTR---KSDAGKYVCVGTNMVGERESEVAELTVLERPS---FVRRPSNMAVTVDDSVEFKCEARGDPVPTVRWRKDDGDLPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASAVLTVQEPPNFVVKPRDQVVALGRTVTFQCEATGNPQPA-IFWQKEGSQNLLFSYQPPQSSSRFSVSQTGDLTITTVQKSDVGYYICQTLNVAGSITTK---------AFLEVTDVVADRPPPVIRQGPVNQTVAVDGTLIMNCIAS----GIPVPTIHWRKDGILIPTQDSRIKQQDSGALQIRYAKLSDTGRYTCTASTTSGEA 497          
BLAST of hemicentin-1 isoform X2 vs. Ensembl Xenopus
Match: lrrc8c (leucine rich repeat containing 8 VRAC subunit C [Source:Xenbase;Acc:XB-GENE-5797483])

HSP 1 Score: 110.923 bits (276), Expect = 7.497e-24
Identity = 145/626 (23.16%), Postives = 251/626 (40.10%), Query Frame = 3
Query:  849 LSCEVYANPAATIQWVIMQGLQIIDSSK----------------FIA-----KIRPDMDGPLICEAKNYLG---STKETVQLNVLRDPLIHIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMTDSS--AVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVE-----TKDIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARW-RYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNV-----VMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQ-----------------PDPPYDLRVTDFEWDSVTINWKPGFNGGLT-QNYEILVISENFGTQNVPFYSDKNENTLTITGLYPSTPYEIRIYAVNSIGRSLNSE 2561
            L+C+    P  TI+W   +G   +++ K                F+      KIRPD +G  +C A+NYLG   S   ++++ +LRD      S+  V       + C+     P   I W K     D      +     KL      +S  G Y CV  N+     G+R+   +E    LTV  +P   +   N  +++    +F  KC    ++ G+P PT++W   +     ++  + D  T ++    +   G Y C+AEN +GK+    +A++TV E P+ + + +++ V L      ++++F CE  G P     W + G++ +      F  +  ++ S   +SQ    LTI          T+++ S  G Y C+  N  G  +   FL +      +     R+    +NQ V     +++ C A  I    I W K  + I++P Q  +    ++                    L I+  +  D   Y+C AS   G  + N+    + Q                 P  P    VTD   ++VT++W+P  N G T  +Y I   S   G+       +   ++  +  L P+  Y   + AVN+ G S  S+
Sbjct:   40 LNCKAEGRPTPTIEW--YKGGDKVETDKDDPRSHRMLLPSGSLFFLRIVHGRKIRPD-EGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGTPIDDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNM----VGERESEVAE----LTVLERPSFVRRPSNMAVTVDDSVEF--KC----EARGDPVPTVRWRKDDGDLPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKA--EASAVLTVQEPPNFVVKPRDQVVALG-----RTVTFQCEATGNPQPAIFWQKEGSQNL-----LFSYQPPQSSSRFSVSQT-GDLTI----------TTVQKSDVGYYICQTLNVAGSITTKAFLEVTDVVADRPPPVIRQ--GPVNQTVAVDGTLIMNCIASGIPVPTIHWRK--DGILIPTQDSRIKQQDS------------------GALQIRYAKLSDTGRYTCTASTTSG--EANWSAYIEVQEFGVPVQPPRATDPNLIPSAPSKPEVTDVSKNTVTLSWQPNLNSGATPTSYLIEAFSHASGSSWQTVAENVKTDSFAVKALKPNAIYLFLVRAVNAYGLSDPSQ 601          

HSP 2 Score: 74.3294 bits (181), Expect = 1.364e-12
Identity = 100/451 (22.17%), Postives = 163/451 (36.14%), Query Frame = 3
Query: 1041 LIHIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMTDS------SAVLISDDAKLNFILID-----RSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWI-------DVETKDIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVL-----PFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQG--IKKVNFEV--VFKSQPDP 2312
            ++   S+  V K    ++NC+    P P  I+WYK  +  ++      S  ++     L F+ I      R   G+Y CV +N             S +   L   ++     ++      +  GE  +++C   R   G P PT+ W        D + + I        +  +     GKY C+  N +G+  S E A +TV E PS + +  N  V +       S+ F CE RG P+   RWR  +  +P S   ++I D   +   +++                      +   G Y C A+N  G A     L ++ PP      R + +A       V  +C+A       I W K     +L     P  S ++S+ +                    +LTI  ++  D   Y C   N  G    K   EV  V   +P P
Sbjct:   23 IVEHPSDLIVSKGEPATLNCKAEGRPTPT-IEWYKGGDKVETDKDDPRSHRMLLPSGSLFFLRIVHGRKIRPDEGVYVCVARNYLGEAVSHNA---SLEVAILRDDFRQNPSDVM------VAVGEPAVMECQPPR---GHPEPTISWKKDGTPIDDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERES-EVAELTVLERPSFVRRPSNMAVTVD-----DSVEFKCEARGDPVPTVRWRKDDGDLPKSR--YEIRDDHTLKIRKVT----------------------AGDMGSYTCVAENMVGKAEASAVLTVQEPPNFVVKPRDQVVALG---RTVTFQCEATGNPQPAIFWQKEGSQNLLFSYQPPQSSSRFSVSQ------------------TGDLTITTVQKSDVGYYICQTLNVAGSITTKAFLEVTDVVADRPPP 409          

HSP 3 Score: 63.929 bits (154), Expect = 1.719e-9
Identity = 96/429 (22.38%), Postives = 152/429 (35.43%), Query Frame = 3
Query:  390 NSGYYVC--RVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISM----YIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFV--PNTVNAMINQTIDLSCEVYANPAATIQWVIMQG------LQIIDSSKF-IAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDP-LIHIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMT---------DSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVT--YKPKEPQILGNKII--SIMKGEKFIIKCSTDRKSPGEPFPTLKW----IDVETKDI---VSDMETFQVINSESRHNGKYKCLAENEIGKS 1568
            + G YVC  R  L     H  SL V  ++       F  N S  +    E   ++C    G P P IS       + D+  +R+ I               I+  R     + G  +C   N  G      + L +  +P    FV  P+ +   ++ +++  CE   +P  T++W    G       +I D     I K+     G   C A+N +G  + +  L V   P  +    +  V      +  C  T NP P  I W K  +            SS   +S    L    + +S  G Y C T N+  S T K          FL VT     + P ++    +  ++      I+ C       G P PT+ W    I + T+D      D    Q+  ++    G+Y C A    G++
Sbjct:   94 DEGVYVCVARNYLGEAVSHNASLEVAILRD-----DFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGTPIDDDKDERITIRGGKL---------MITYTR---KSDAGKYVCVGTNMVGERESEVAELTVLERPS---FVRRPSNMAVTVDDSVEFKCEARGDPVPTVRWRKDDGDLPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASAVLTVQEPPNFVVKPRDQVVALGRTVTFQCEATGNPQPA-IFWQKEGSQNLLFSYQPPQSSSRFSVSQTGDLTITTVQKSDVGYYICQTLNVAGSITTK---------AFLEVTDVVADRPPPVIRQGPVNQTVAVDGTLIMNCIAS----GIPVPTIHWRKDGILIPTQDSRIKQQDSGALQIRYAKLSDTGRYTCTASTTSGEA 488          
BLAST of hemicentin-1 isoform X2 vs. Ensembl Xenopus
Match: lrrc8c (leucine rich repeat containing 8 VRAC subunit C [Source:Xenbase;Acc:XB-GENE-5797483])

HSP 1 Score: 110.923 bits (276), Expect = 9.069e-24
Identity = 154/679 (22.68%), Postives = 271/679 (39.91%), Query Frame = 3
Query:  705 NRGFVMCTAI-----NDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQWVIMQGLQIIDSSK----------------FIA-----KIRPDMDGPLICEAKNYLG---STKETVQLNVLRDPLIHIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNM--TDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVE-----TKDIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARW-RYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNV-----VMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQ-----------------PDPPYDLRVTDFEWDSVTINWKPGFNGGLT-QNYEILVISENFGTQNVPFYSDKNENTLTITGLYPSTPYEIRIYAVNSIGRSLNSE 2561
            +RG    TA+     +D  ++   + L    + P+I    P+ +     +   L+C+    P  TI+W   +G   +++ K                F+      KIRPD +G  +C A+NYLG   S   ++++ +LRD      S+  V       + C+     P   I W K       D    +     KL      +S  G Y CV  N+     G+R+   +E    LTV  +P   +   N  +++    +F  KC    ++ G+P PT++W   +     ++  + D  T ++    +   G Y C+AEN +GK+    +A++TV E P+ + + +++ V L      ++++F CE  G P     W + G++ +      F  +  ++ S   +SQ    LTI          T+++ S  G Y C+  N  G  +   FL +      +     R+    +NQ V     +++ C A  I    I W K  + I++P Q  +    ++                    L I+  +  D   Y+C AS   G  + N+    + Q                 P  P    VTD   ++VT++W+P  N G T  +Y I   S   G+       +   ++  +  L P+  Y   + AVN+ G S  S+
Sbjct:   37 DRGLDSGTALPTSDHDDNSLVYTGSRLRQEDFPPRIVEH-PSDLIVSKGEPATLNCKAEGRPTPTIEW--YKGGDKVETDKDDPRSHRMLLPSGSLFFLRIVHGRKIRPD-EGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGTPIDDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNM----VGERESEVAE----LTVLERPSFVRRPSNMAVTVDDSVEF--KC----EARGDPVPTVRWRKDDGDLPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKA--EASAVLTVQEPPNFVVKPRDQVVALG-----RTVTFQCEATGNPQPAIFWQKEGSQNL-----LFSYQPPQSSSRFSVSQT-GDLTI----------TTVQKSDVGYYICQTLNVAGSITTKAFLEVTDVVADRPPPVIRQ--GPVNQTVAVDGTLIMNCIASGIPVPTIHWRK--DGILIPTQDSRIKQQDS------------------GALQIRYAKLSDTGRYTCTASTTSG--EANWSAYIEVQEFGVPVQPPRATDPNLIPSAPSKPEVTDVSKNTVTLSWQPNLNSGATPTSYLIEAFSHASGSSWQTVAENVKTDSFAVKALKPNAIYLFLVRAVNAYGLSDPSQ 650          

HSP 2 Score: 64.3142 bits (155), Expect = 1.615e-9
Identity = 96/429 (22.38%), Postives = 152/429 (35.43%), Query Frame = 3
Query:  390 NSGYYVC--RVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISM----YIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFV--PNTVNAMINQTIDLSCEVYANPAATIQWVIMQG------LQIIDSSKF-IAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDP-LIHIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMT---------DSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVT--YKPKEPQILGNKII--SIMKGEKFIIKCSTDRKSPGEPFPTLKW----IDVETKDI---VSDMETFQVINSESRHNGKYKCLAENEIGKS 1568
            + G YVC  R  L     H  SL V  ++       F  N S  +    E   ++C    G P P IS       + D+  +R+ I               I+  R     + G  +C   N  G      + L +  +P    FV  P+ +   ++ +++  CE   +P  T++W    G       +I D     I K+     G   C A+N +G  + +  L V   P  +    +  V      +  C  T NP P  I W K  +            SS   +S    L    + +S  G Y C T N+  S T K          FL VT     + P ++    +  ++      I+ C       G P PT+ W    I + T+D      D    Q+  ++    G+Y C A    G++
Sbjct:  143 DEGVYVCVARNYLGEAVSHNASLEVAILRD-----DFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGTPIDDDKDERITIRGGKL---------MITYTR---KSDAGKYVCVGTNMVGERESEVAELTVLERPS---FVRRPSNMAVTVDDSVEFKCEARGDPVPTVRWRKDDGDLPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASAVLTVQEPPNFVVKPRDQVVALGRTVTFQCEATGNPQPA-IFWQKEGSQNLLFSYQPPQSSSRFSVSQTGDLTITTVQKSDVGYYICQTLNVAGSITTK---------AFLEVTDVVADRPPPVIRQGPVNQTVAVDGTLIMNCIAS----GIPVPTIHWRKDGILIPTQDSRIKQQDSGALQIRYAKLSDTGRYTCTASTTSGEA 537          
BLAST of hemicentin-1 isoform X2 vs. Ensembl Xenopus
Match: lrrc8c (leucine rich repeat containing 8 VRAC subunit C [Source:Xenbase;Acc:XB-GENE-5797483])

HSP 1 Score: 110.538 bits (275), Expect = 1.072e-23
Identity = 154/679 (22.68%), Postives = 271/679 (39.91%), Query Frame = 3
Query:  705 NRGFVMCTAI-----NDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQWVIMQGLQIIDSSK----------------FIA-----KIRPDMDGPLICEAKNYLG---STKETVQLNVLRDPLIHIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNM--TDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVE-----TKDIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARW-RYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNV-----VMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQ-----------------PDPPYDLRVTDFEWDSVTINWKPGFNGGLT-QNYEILVISENFGTQNVPFYSDKNENTLTITGLYPSTPYEIRIYAVNSIGRSLNSE 2561
            +RG    TA+     +D  ++   + L    + P+I    P+ +     +   L+C+    P  TI+W   +G   +++ K                F+      KIRPD +G  +C A+NYLG   S   ++++ +LRD      S+  V       + C+     P   I W K       D    +     KL      +S  G Y CV  N+     G+R+   +E    LTV  +P   +   N  +++    +F  KC    ++ G+P PT++W   +     ++  + D  T ++    +   G Y C+AEN +GK+    +A++TV E P+ + + +++ V L      ++++F CE  G P     W + G++ +      F  +  ++ S   +SQ    LTI          T+++ S  G Y C+  N  G  +   FL +      +     R+    +NQ V     +++ C A  I    I W K  + I++P Q  +    ++                    L I+  +  D   Y+C AS   G  + N+    + Q                 P  P    VTD   ++VT++W+P  N G T  +Y I   S   G+       +   ++  +  L P+  Y   + AVN+ G S  S+
Sbjct:   37 DRGLDSGTALPTSDHDDNSLVYTGSRLRQEDFPPRIVEH-PSDLIVSKGEPATLNCKAEGRPTPTIEW--YKGGDKVETDKDDPRSHRMLLPSGSLFFLRIVHGRKIRPD-EGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGTPIDDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNM----VGERESEVAE----LTVLERPSFVRRPSNMAVTVDDSVEF--KC----EARGDPVPTVRWRKDDGDLPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKA--EASAVLTVQEPPNFVVKPRDQVVALG-----RTVTFQCEATGNPQPAIFWQKEGSQNL-----LFSYQPPQSSSRFSVSQT-GDLTI----------TTVQKSDVGYYICQTLNVAGSITTKAFLEVTDVVADRPPPVIRQ--GPVNQTVAVDGTLIMNCIASGIPVPTIHWRK--DGILIPTQDSRIKQQDS------------------GALQIRYAKLSDTGRYTCTASTTSG--EANWSAYIEVQEFGVPVQPPRATDPNLIPSAPSKPEVTDVSKNTVTLSWQPNLNSGATPTSYLIEAFSHASGSSWQTVAENVKTDSFAVKALKPNAIYLFLVRAVNAYGLSDPSQ 650          

HSP 2 Score: 64.3142 bits (155), Expect = 1.587e-9
Identity = 96/429 (22.38%), Postives = 152/429 (35.43%), Query Frame = 3
Query:  390 NSGYYVC--RVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISM----YIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFV--PNTVNAMINQTIDLSCEVYANPAATIQWVIMQG------LQIIDSSKF-IAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDP-LIHIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMT---------DSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVT--YKPKEPQILGNKII--SIMKGEKFIIKCSTDRKSPGEPFPTLKW----IDVETKDI---VSDMETFQVINSESRHNGKYKCLAENEIGKS 1568
            + G YVC  R  L     H  SL V  ++       F  N S  +    E   ++C    G P P IS       + D+  +R+ I               I+  R     + G  +C   N  G      + L +  +P    FV  P+ +   ++ +++  CE   +P  T++W    G       +I D     I K+     G   C A+N +G  + +  L V   P  +    +  V      +  C  T NP P  I W K  +            SS   +S    L    + +S  G Y C T N+  S T K          FL VT     + P ++    +  ++      I+ C       G P PT+ W    I + T+D      D    Q+  ++    G+Y C A    G++
Sbjct:  143 DEGVYVCVARNYLGEAVSHNASLEVAILRD-----DFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGTPIDDDKDERITIRGGKL---------MITYTR---KSDAGKYVCVGTNMVGERESEVAELTVLERPS---FVRRPSNMAVTVDDSVEFKCEARGDPVPTVRWRKDDGDLPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASAVLTVQEPPNFVVKPRDQVVALGRTVTFQCEATGNPQPA-IFWQKEGSQNLLFSYQPPQSSSRFSVSQTGDLTITTVQKSDVGYYICQTLNVAGSITTK---------AFLEVTDVVADRPPPVIRQGPVNQTVAVDGTLIMNCIAS----GIPVPTIHWRKDGILIPTQDSRIKQQDSGALQIRYAKLSDTGRYTCTASTTSGEA 537          
BLAST of hemicentin-1 isoform X2 vs. Ensembl Xenopus
Match: lrrc8c (leucine rich repeat containing 8 VRAC subunit C [Source:Xenbase;Acc:XB-GENE-5797483])

HSP 1 Score: 110.538 bits (275), Expect = 1.312e-23
Identity = 154/679 (22.68%), Postives = 272/679 (40.06%), Query Frame = 3
Query:  705 NRGFVMCTAI-----NDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQWVIMQGLQIIDSSK----------------FIA-----KIRPDMDGPLICEAKNYLG---STKETVQLNVLRDPLIHIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNM--TDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVE-----TKDIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARW-RYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNV-----VMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQ-----------------PDPPYDLRVTDFEWDSVTINWKPGFNGGLT-QNYEILVISENFGTQNVPFYSDKNENTLTITGLYPSTPYEIRIYAVNSIGRSLNSE 2561
            +RG    TA+     +D  ++   + L    + P+I    P+ +     +   L+C+    P  TI+W   +G   +++ K                F+      KIRPD +G  +C A+NYLG   S   ++++ +LRD      S+  V       + C+     P   I W K       D    +     KL      +S  G Y CV  N+     G+R+   +E    LTV  +P   +   N  +++    +F  KC    ++ G+P PT++W   +     ++  + D  T ++    +   G Y C+AEN +GK+ +  +A++TV E P+ + + +++ V L      ++++F CE  G P     W + G++ +      F  +  ++ S   +SQ    LTI          T+++ S  G Y C+  N  G  +   FL +      +     R+    +NQ V     +++ C A  I    I W K  + I++P Q  +    ++                    L I+  +  D   Y+C AS   G  + N+    + Q                 P  P    VTD   ++VT++W+P  N G T  +Y I   S   G+       +   ++  +  L P+  Y   + AVN+ G S  S+
Sbjct:   31 DRGLDSGTALPTSDHDDNSLVYTGSRLRQEDFPPRIVEH-PSDLIVSKGEPATLNCKAEGRPTPTIEW--YKGGDKVETDKDDPRSHRMLLPSGSLFFLRIVHGRKIRPD-EGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGTPIDDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNM----VGERESEVAE----LTVLERPSFVRRPSNMAVTVDDSVEF--KC----EARGDPVPTVRWRKDDGDLPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEA--SAVLTVQEPPNFVVKPRDQVVALG-----RTVTFQCEATGNPQPAIFWQKEGSQNL-----LFSYQPPQSSSRFSVSQT-GDLTI----------TTVQKSDVGYYICQTLNVAGSITTKAFLEVTDVVADRPPPVIRQ--GPVNQTVAVDGTLIMNCIASGIPVPTIHWRK--DGILIPTQDSRIKQQDS------------------GALQIRYAKLSDTGRYTCTASTTSG--EANWSAYIEVQEFGVPVQPPRATDPNLIPSAPSKPEVTDVSKNTVTLSWQPNLNSGATPTSYLIEAFSHASGSSWQTVAENVKTDSFAVKALKPNAIYLFLVRAVNAYGLSDPSQ 644          

HSP 2 Score: 64.3142 bits (155), Expect = 1.755e-9
Identity = 96/429 (22.38%), Postives = 152/429 (35.43%), Query Frame = 3
Query:  390 NSGYYVC--RVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISM----YIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFV--PNTVNAMINQTIDLSCEVYANPAATIQWVIMQG------LQIIDSSKF-IAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDP-LIHIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMT---------DSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVT--YKPKEPQILGNKII--SIMKGEKFIIKCSTDRKSPGEPFPTLKW----IDVETKDI---VSDMETFQVINSESRHNGKYKCLAENEIGKS 1568
            + G YVC  R  L     H  SL V  ++       F  N S  +    E   ++C    G P P IS       + D+  +R+ I               I+  R     + G  +C   N  G      + L +  +P    FV  P+ +   ++ +++  CE   +P  T++W    G       +I D     I K+     G   C A+N +G  + +  L V   P  +    +  V      +  C  T NP P  I W K  +            SS   +S    L    + +S  G Y C T N+  S T K          FL VT     + P ++    +  ++      I+ C       G P PT+ W    I + T+D      D    Q+  ++    G+Y C A    G++
Sbjct:  137 DEGVYVCVARNYLGEAVSHNASLEVAILRD-----DFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGTPIDDDKDERITIRGGKL---------MITYTR---KSDAGKYVCVGTNMVGERESEVAELTVLERPS---FVRRPSNMAVTVDDSVEFKCEARGDPVPTVRWRKDDGDLPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASAVLTVQEPPNFVVKPRDQVVALGRTVTFQCEATGNPQPA-IFWQKEGSQNLLFSYQPPQSSSRFSVSQTGDLTITTVQKSDVGYYICQTLNVAGSITTK---------AFLEVTDVVADRPPPVIRQGPVNQTVAVDGTLIMNCIAS----GIPVPTIHWRKDGILIPTQDSRIKQQDSGALQIRYAKLSDTGRYTCTASTTSGEA 531          
BLAST of hemicentin-1 isoform X2 vs. Ensembl Mouse
Match: Robo1 (roundabout guidance receptor 1 [Source:MGI Symbol;Acc:MGI:1274781])

HSP 1 Score: 107.457 bits (267), Expect = 9.081e-23
Identity = 148/617 (23.99%), Postives = 246/617 (39.87%), Query Frame = 3
Query:  849 LSCEVYANPAATIQWVIMQGLQIIDSSK---------------FIAKI------RPDMDGPLICEAKNYLG---STKETVQLNVLRDPLIHIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYK-LSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWI--DVE---TKDIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRY-GNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNV-----VMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQG-------IKKVNFEVVFKSQ--------PDPPYDLRVTDFEWDSVTINWKPGFNGGLT-QNYEILVISENFGTQNVPFYSDKNENTLTITGLYPSTPYEIRIYAVNSIG 2543
            L+C+    P  TI+W   +G + +++ K               F  +I      RPD +G  IC A+NYLG   S   ++++ +LRD      S+  V       + C+     P   I W K  S + D    +     KL      +S  G Y CV  N+     G+R+   +E    LTV  +P   +   N  +++    +F  KC    ++ G+P PT++W   D E   ++  + D  T ++    +   G Y C+AEN +GK  +  +A +TV E P  + + +++ V L      ++++F CE  G P     WR  G++ +      F  +  ++ S   +SQ    LTI          T+++ S  G Y C+  N  G      +L +      +     R+    +NQ V     +++ C A       I W K  + +++  Q  +    E+ V                  L I+  +  D   Y+C AS   G       I+   F V  +          P  P    VTD   ++VT++W+P  N G T  +Y I   S   G+       +    T  I GL P+  Y   + A N+ G
Sbjct:   48 LNCKAEGRPTPTIEW--YKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPD-EGVYICVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNM----VGERESEVAE----LTVLERPSFVKRPSNLAVTVDDSAEF--KC----EARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGK--AEASATLTVQEPPHFVVKPRDQVVALG-----RTVTFQCEATGNPQPAIFWRREGSQNL-----LFSYQPPQSSSRFSVSQT-GDLTI----------TNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQ--GPVNQTVAVDGTLILSCVATGSPAPTILWRK--DGVLVSTQDSRIKQLESGV------------------LQIRYAKLGDTGRYTCTASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSKNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTAAENVKTETFAIKGLKPNAIYLFLVRAANAYG 602          

HSP 2 Score: 76.6406 bits (187), Expect = 2.022e-13
Identity = 100/450 (22.22%), Postives = 165/450 (36.67%), Query Frame = 3
Query: 1041 LIHIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMTDS------SAVLISDDAKLNFILI-----DRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKW------IDVETKDIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVL-----PFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQG--IKKVNFEV--VFKSQPDP 2312
            ++   S+  V K    ++NC+    P P  I+WYK     ++      S  ++     L F+ I      R   G+Y CV +N             S +   L   ++     ++      +  GE  +++C   R   G P PT+ W      +D + + I        +  +     GKY C+  N +G+  S E A +TV E PS + +  N  V +       S  F CE RG P+   RWR  + ++P S   ++I D   +   +++                      +   G Y C A+N  G A     L ++ PP      R + +A       V  +C+A       I W +     +L     P  S ++S+ +                    +LTI +++  D   Y C   N  G  I K   EV  V   +P P
Sbjct:   31 IVEHPSDLIVSKGEPATLNCKAEGRPTPT-IEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYICVARNYLGEAVSHNA---SLEVAILRDDFRQNPSDVM------VAVGEPAVMECQPPR---GHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERES-EVAELTVLERPSFVKRPSNLAVTVD-----DSAEFKCEARGDPVPTVRWRKDDGELPKSR--YEIRDDHTLKIRKVT----------------------AGDMGSYTCVAENMVGKAEASATLTVQEPPHFVVKPRDQVVALG---RTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQ------------------TGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPP 416          

HSP 3 Score: 58.5362 bits (140), Expect = 6.839e-8
Identity = 97/434 (22.35%), Postives = 155/434 (35.71%), Query Frame = 3
Query:  360 RLTIHNISSDNSGYYVC--RVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVK---DNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFV--PNTVNAMINQTIDLSCEVYANPAATIQWVIMQG------LQIIDSSKF-IAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLIHIDSNYRVPK-NSNYSINCRVTSNPPPEFIKWYKLSNMT---------DSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKW----IDVETKDI-VSDMET--FQVINSESRHNGKYKCLAENEIGKS 1568
            R+     S  + G Y+C  R  L     H  SL V  ++       F  N S  +    E   ++C    G P P IS        D+  +R+ I               I+  R     + G  +C   N  G      + L +  +P    FV  P+ +   ++ + +  CE   +P  T++W    G       +I D     I K+     G   C A+N +G  + +  L V   P   +    +V       +  C  T NP P  I W +  +            SS   +S    L    + RS  G Y C T N+  S   K   A  E T  +     P   Q   N+ +++      I+ C       G P PT+ W    + V T+D  +  +E+   Q+  ++    G+Y C A    G++
Sbjct:   92 RIVHGRKSRPDEGVYICVARNYLGEAVSHNASLEVAILRD-----DFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKL---------MITYTR---KSDAGKYVCVGTNMVGERESEVAELTVLERPS---FVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPA-IFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITK---AYLEVTDVIADRPPPVIRQGPVNQTVAV--DGTLILSCVAT----GSPAPTILWRKDGVLVSTQDSRIKQLESGVLQIRYAKLGDTGRYTCTASTPSGEA 495          
BLAST of hemicentin-1 isoform X2 vs. Ensembl Mouse
Match: Robo1 (roundabout guidance receptor 1 [Source:MGI Symbol;Acc:MGI:1274781])

HSP 1 Score: 106.301 bits (264), Expect = 1.777e-22
Identity = 148/617 (23.99%), Postives = 246/617 (39.87%), Query Frame = 3
Query:  849 LSCEVYANPAATIQWVIMQGLQIIDSSK---------------FIAKI------RPDMDGPLICEAKNYLG---STKETVQLNVLRDPLIHIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYK-LSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWI--DVE---TKDIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRY-GNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNV-----VMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQG-------IKKVNFEVVFKSQ--------PDPPYDLRVTDFEWDSVTINWKPGFNGGLT-QNYEILVISENFGTQNVPFYSDKNENTLTITGLYPSTPYEIRIYAVNSIG 2543
            L+C+    P  TI+W   +G + +++ K               F  +I      RPD +G  IC A+NYLG   S   ++++ +LRD      S+  V       + C+     P   I W K  S + D    +     KL      +S  G Y CV  N+     G+R+   +E    LTV  +P   +   N  +++    +F  KC    ++ G+P PT++W   D E   ++  + D  T ++    +   G Y C+AEN +GK  +  +A +TV E P  + + +++ V L      ++++F CE  G P     WR  G++ +      F  +  ++ S   +SQ    LTI          T+++ S  G Y C+  N  G      +L +      +     R+    +NQ V     +++ C A       I W K  + +++  Q  +    E+ V                  L I+  +  D   Y+C AS   G       I+   F V  +          P  P    VTD   ++VT++W+P  N G T  +Y I   S   G+       +    T  I GL P+  Y   + A N+ G
Sbjct:   58 LNCKAEGRPTPTIEW--YKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPD-EGVYICVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNM----VGERESEVAE----LTVLERPSFVKRPSNLAVTVDDSAEF--KC----EARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGK--AEASATLTVQEPPHFVVKPRDQVVALG-----RTVTFQCEATGNPQPAIFWRREGSQNL-----LFSYQPPQSSSRFSVSQT-GDLTI----------TNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQ--GPVNQTVAVDGTLILSCVATGSPAPTILWRK--DGVLVSTQDSRIKQLESGV------------------LQIRYAKLGDTGRYTCTASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSKNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTAAENVKTETFAIKGLKPNAIYLFLVRAANAYG 612          

HSP 2 Score: 77.7962 bits (190), Expect = 9.466e-14
Identity = 105/468 (22.44%), Postives = 172/468 (36.75%), Query Frame = 3
Query:  987 NYLGSTKETVQLNVLRDPLIHIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMTDS------SAVLISDDAKLNFILI-----DRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKW------IDVETKDIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVL-----PFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQG--IKKVNFEV--VFKSQPDP 2312
            N LG T   ++       ++   S+  V K    ++NC+    P P  I+WYK     ++      S  ++     L F+ I      R   G+Y CV +N             S +   L   ++     ++      +  GE  +++C   R   G P PT+ W      +D + + I        +  +     GKY C+  N +G+  S E A +TV E PS + +  N  V +       S  F CE RG P+   RWR  + ++P S   ++I D   +   +++        D+                G Y C A+N  G A     L ++ PP      R + +A       V  +C+A       I W +     +L     P  S ++S+ +                    +LTI +++  D   Y C   N  G  I K   EV  V   +P P
Sbjct:   23 NSLGYTGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPT-IEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYICVARNYLGEAVSHNA---SLEVAILRDDFRQNPSDVM------VAVGEPAVMECQPPR---GHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERES-EVAELTVLERPSFVKRPSNLAVTVD-----DSAEFKCEARGDPVPTVRWRKDDGELPKSR--YEIRDDHTLKIRKVT------AGDM----------------GSYTCVAENMVGKAEASATLTVQEPPHFVVKPRDQVVALG---RTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQ------------------TGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPP 426          

HSP 3 Score: 58.151 bits (139), Expect = 8.746e-8
Identity = 97/434 (22.35%), Postives = 155/434 (35.71%), Query Frame = 3
Query:  360 RLTIHNISSDNSGYYVC--RVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVK---DNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFV--PNTVNAMINQTIDLSCEVYANPAATIQWVIMQG------LQIIDSSKF-IAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLIHIDSNYRVPK-NSNYSINCRVTSNPPPEFIKWYKLSNMT---------DSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKW----IDVETKDI-VSDMET--FQVINSESRHNGKYKCLAENEIGKS 1568
            R+     S  + G Y+C  R  L     H  SL V  ++       F  N S  +    E   ++C    G P P IS        D+  +R+ I               I+  R     + G  +C   N  G      + L +  +P    FV  P+ +   ++ + +  CE   +P  T++W    G       +I D     I K+     G   C A+N +G  + +  L V   P   +    +V       +  C  T NP P  I W +  +            SS   +S    L    + RS  G Y C T N+  S   K   A  E T  +     P   Q   N+ +++      I+ C       G P PT+ W    + V T+D  +  +E+   Q+  ++    G+Y C A    G++
Sbjct:  102 RIVHGRKSRPDEGVYICVARNYLGEAVSHNASLEVAILRD-----DFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKL---------MITYTR---KSDAGKYVCVGTNMVGERESEVAELTVLERPS---FVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPA-IFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITK---AYLEVTDVIADRPPPVIRQGPVNQTVAVDG--TLILSCVAT----GSPAPTILWRKDGVLVSTQDSRIKQLESGVLQIRYAKLGDTGRYTCTASTPSGEA 505          
BLAST of hemicentin-1 isoform X2 vs. Ensembl Mouse
Match: Hmcn1 (hemicentin 1 [Source:MGI Symbol;Acc:MGI:2685047])

HSP 1 Score: 103.219 bits (256), Expect = 1.861e-21
Identity = 168/689 (24.38%), Postives = 265/689 (38.46%), Query Frame = 3
Query:  363 LTIHNISSDNSGYYVCRVTLLTTSEHIISLAVVTVKTPPKSIS-FHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSA---ISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNT---VNAMINQTIDLSCEVYANPAATIQWVIMQGLQIIDSSKF----------IAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLIHI-DSNYRVPKNSN-YSINCRVTSNPPPEFIKWYK----LSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKI---ISIMKGEKFIIKCSTDRKSPGEPFPTLKWI---------DVETKDIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVN--EAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQPDPPY 2318
            L I N    ++G YVC      T+        + V  PP  I   H + S+ V     FI++ C VS G PPP +S ++  +  IK         PN+ +  V     + + R   + + G   C AIN AG   K  S L +   P IK     +   VN     ++ L CE  A P   I W    G  I DS+            I +      G  +C A N  G   +   LNV   P I   ++   V   SN  ++ C  T  PPP  I W K    + N       ++S  +KL      RS++G Y CV  N++    GK   A     +F+ V      P + G ++   +S++ GE   + C+ D    G P P L+W+         + E   + +D  T  +  + +   GKY C+A N  G+    E  +  +N    P I    +  +  ++  D   SI+  C+  G P  +  W      +P SS        R +S  ++ +I      D+                  Y C A NR G+ S  Y L +  PP +   +   +I      +  M  C  +      + W +  + +            +A +T       + T G+    L +   E +D   Y+CVA+N+ G  +V+   V K   +PP+
Sbjct: 2927 LQILNAQITDTGRYVCVAE--NTAGSAKKYFNLNVHVPPSVIGPNHEHLSVVV---NHFISLNCEVS-GFPPPDLS-WLKNEEPIK---------PNTNVLTVPGGRTLQIIRAK-ISDGGDYTCIAINQAGESKKKVS-LTVHVPPSIKDHGSQSLSIVNVREGTSVSLECESNAVPPPVITWS-KNGRMIPDSTNVEILTGGQTLHIRRAEVSDTGQYVCRAINVAGRDDKNFHLNVYVPPTIEGPETEVIVETISNPVTLTCDATGIPPPT-ITWLKNHKPIENSDPLEVHILSGGSKLQIARPQRSNSGNYTCVASNME----GK---AQKNFILFIQV-----PPSVAGAEVPSEVSVLLGENVELVCNAD----GIPTPHLQWLRDGKPIVNGETERVRVTTDGSTLNIYRALTSDMGKYTCVATNPAGE----EDRIFNLNVYVPPKIRGNKEEAEKLMALVD--TSINIECKATGTPPPQINWLKNGLPLPISSH------IRLLSAGQVVRIVRAQVSDI----------------AVYTCVASNRAGVDSKHYSLQVFVPPNMDNAMGTEEITIVKGSSTSMT-CFTDGTPAPSMSWLRDGQPLAP----------DAHLT-------VSTQGMV---LQLIKAETEDTGKYTCVATNEAG--EVSKHFVLKVL-EPPH 3527          

HSP 2 Score: 103.219 bits (256), Expect = 2.221e-21
Identity = 154/697 (22.09%), Postives = 258/697 (37.02%), Query Frame = 3
Query:  315 VVPDSESQITISHESRLTIHNISSDNSGYYVCRVTLLTTSE----HIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNST--IAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPF-VPNTVNAMINQTIDLSCEVYANPAATIQWVIMQGLQIIDSSKFIAKIRPDMD------------GPLICEAKNYLGSTKETVQLNVLRDPLIHID----SNYRVPKNSNYSINCRVTSNPPPEFIKWYK--LSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETKDIVSDME--------TFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQP 2306
            ++PDS +   ++    L I      ++G YVCR   +   +    H+      T++ P           + V      + + C  +G IPPP I+        +K  K I ++ P     ++  S + + RP    N G   C A N  G   KN  +L I+  P +    VP+ V+ ++ + ++L C     P   +QW +  G  I++      ++  D              G   C A N  G       LNV   P I  +           +++ +I C+ T  PPP+ I W K  L     S   L+S    +  +    S   +Y CV  N         +     K   L V   P     +G + I+I+KG    + C TD    G P P++ W+  + + +  D            Q+I +E+   GKY C+A NE G+  S+   L  V E P I       +V +  ++    +  +C   G P  +  W               ++D R         + T+    L      + +S +    GRY C A +  G    +Y + +  PP I      +      N+ V +  C+++++    I W K  E +                 +  P   + + G     L I + +  D +NY+CVASN  G     F +     P
Sbjct: 3098 MIPDSTNVEILTGGQTLHIRRAEVSDTGQYVCRAINVAGRDDKNFHLNVYVPPTIEGP--------ETEVIVETISNPVTLTCDATG-IPPPTITW-------LKNHKPIENSDPLEVHILSGGSKLQIARPQR-SNSGNYTCVASNMEGKAQKNF-ILFIQVPPSVAGAEVPSEVSVLLGENVELVCNADGIPTPHLQW-LRDGKPIVNGETERVRVTTDGSTLNIYRALTSDMGKYTCVATNPAGEEDRIFNLNVYVPPKIRGNKEEAEKLMALVDTSINIECKATGTPPPQ-INWLKNGLPLPISSHIRLLSAGQVVRIVRAQVSDIAVYTCVASN---------RAGVDSKHYSLQVFVPPNMDNAMGTEEITIVKGSSTSMTCFTD----GTPAPSMSWLR-DGQPLAPDAHLTVSTQGMVLQLIKAETEDTGKYTCVATNEAGE-VSKHFVL-KVLEPPHINGSEGPGEVSVIVNN---PLELSCIASGIPAPKISW---------------MKDGRPF-------LQTEQVQTLEGGAILRVSSAQVEDTGRYTCLASSPAGDDDKEYLVRVHVPPNIAGMDEAQDFTVLRNRQVTL-ECKSDAVPPPVIMWLKNREQL-----------------QATPRVRILSGG---RYLQINNADLGDTANYTCVASNIAGKTTREFNLTVNVPP 3712          

HSP 3 Score: 99.7525 bits (247), Expect = 2.413e-20
Identity = 151/698 (21.63%), Postives = 266/698 (38.11%), Query Frame = 3
Query:  315 VVPDSESQITI-SHESRLTIHNISSDNSGYYVC---RVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKI--KPFVPNTVNAMINQTIDLSCEVYANPAATIQWV-------IMQGLQIIDSSKFI--AKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLIHIDS---NYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNM--TDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETK-------DIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLP----QSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDY---------FLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEV 2288
            V+ DS  ++ I S   RL I      ++G Y C    V  +   E+ + + +     P  + S      I V + +  I+++C V G IP P ++ ++     + + K +        I +   I   +  ++ + G  +C A+N AG+  K   L ++   P I     VP  V+ +   ++ L+CE    P  +I W+       +   ++I+   + +   + RP+  G   C  +N  G  ++   L+VL  P I  ++   + ++ +  + ++ C V  NP P+ I W+K   +   D +  ++S    L       S TG Y C+  NI        K  +    +F++ T    +       +I I+     ++ C      P     T+ W    T         I+    T Q++N++  + G+Y C+A NE G+        V +   P II +       LS  ++      S++  CE    P    RW           Q  K +DH              +TI     T  Q    + S  GRY C A N  G   LD+         F  +     +    R  +    +  N + + C+  +     + WYK       P  S               +R L  PG +V  L I   + +D   Y+CVA N+ G   + ++V
Sbjct: 2236 VLADSAGRVHILSGGRRLQISIAEKADAGLYTCVASNVAGVAKKEYNLQVYI----RPSITNSGGHRPEITVIRGKS-ISLECEVQG-IPQPTVT-WMKDGRPLTKGKGVE-------ILDEGRILQLKNVHVSDTGRYVCVAVNVAGMTDKRYDL-SVHAPPSIIGNHGVPENVSVVEKSSVSLTCEASGIPLPSITWLKDGWPVNLGSSVKILSGGRMLRLMQTRPEDAGQYTCIVRNAAGEDRKMFGLSVLVPPHIVGENTLEDVKIKEKQSVTLTCEVRGNPVPQ-ITWHKDGQLLQEDEAHHMMSGGRFLQITNAQVSHTGRYTCLASNI-----AGDKSKSFRLNVFVSPTIAGVDSDGSPEDVIVILNSPTSLV-CEAYSYPPA----TITWFKDGTPLESNRNIRILPGGRTLQILNAQEDNAGRYSCVATNEAGEKIKHYEVKVYI---PPIIKKGDLLGPGLSPKEVKIRVNSSLTLECEAYAIPSASLRWYK-------DGQPLKSDDH--------------VTIAASGHTL-QIKEAQISDTGRYTCVASNLAGEDELDFDVNIQVPPSFQKLWEIGNMLDTGRSGEAKDVIINNPLSLHCETNAAPPPTLTWYKDGR----PLTSS--------------DRVLILPGGRV--LQIPRAKVEDAGRYTCVAVNEAGEDSLRYDV 2862          

HSP 4 Score: 93.5893 bits (231), Expect = 1.866e-18
Identity = 152/679 (22.39%), Postives = 265/679 (39.03%), Query Frame = 3
Query:  363 LTIHNISSDNSGYYVCRVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRL------KIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQWVIMQGLQIIDSSKFIA-----------KIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLIHI-DSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSN-MTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKI-ISIMKGEKFIIKCSTDRKSPGEPFPTLKW-------IDVETKDIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRET----ALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQP 2306
            L I + S D++  Y C VT  + +        +TV+ PP +I+   ++ ++    ++  A+    + G+P P I       N ++ L      +I+SS           AI +     L + G   C A N AG   ++ +L  ++  P I+P  P+ ++ ++N  I L CE    P   I W   +G+ +I S K +A            +R D  G  +C A+N  G+    V+LNV   P+I      Y V  +   S+ C    +PPP+ I W+K  + +T+S    I +   L          G Y C+  N+           +S  +  LTV   P+   I   ++  ++ +  + ++ C  D    G P P + W        ++  K          + N     +G Y C+A N  G+     T     L T  E P  ++ +K  ++ LS           C+  G PL +  W + N  IP  +    I  H  +  E++S+                        +G Y C A+N  G      F+ +K PP+ K +     I   L  N ++  C+ +      I+W +    I +  + ++                     L    L I    N+D  +Y+CVA+N+ G+ + +  +  +S P
Sbjct: 3860 LVIISPSVDDTASYECTVT--SDAGEDKRAVDLTVQVPP-TIA---DEPMDFLVTRQAPAVMTCSASGVPVPSIHW---TKNGLRLLPRGDGYRILSSG----------AIEIPTTQ-LNHAGRYTCVARNAAGSAHRHVTL-RVQEPPVIQP-QPSELDVILNNPILLPCEATGIPTPFITWQ-KEGINVITSGKSLAILPSGSLQISRAVRGDA-GTYMCVAQNPAGTALGKVKLNVQVPPVISSHQKEYVVTMDKPVSLLCETEGSPPPD-ITWHKDGHALTESIRQRILNSGALQIAFAQPDDAGQYTCMAANMA---------GSSSVSSTLTVHVPPR---IQSTEVHFTVNENSQAVLPCVAD----GIPTPAIHWEKDGVLIANLLGKYTAQPYGELILENVVLEDSGTYTCVANNAAGEDTRIVTLAVHTLPTFTELPGDLSLNKGEQLRLS-----------CKAVGIPLPKLTWTFNNNIIP--AHFDSINGHSELVIEKVSK----------------------EDSGTYVCTAENSVGFVKAIGFVYVKEPPVFKGDYPSNWIEP-LGGNAIL-NCEVKGDPAPTIQWSRKGADIEISHRIRQ---------------------LGNGSLAIYGTVNEDAGDYTCVAANEAGMVERSMSLTLQSSP 4439          

HSP 5 Score: 90.5077 bits (223), Expect = 1.351e-17
Identity = 130/553 (23.51%), Postives = 217/553 (39.24%), Query Frame = 3
Query:  699 LENRGFVMCTAINDAGVLTKNTSL---LNIKYKPKIKPF---VPNTVNAMINQTIDLSCEVYANPAATIQWVIMQGLQII---DSSKFIAKIRPDM--------DGPLICEAKNYLGSTKETVQLNVLRDPLIHIDSNYR---VPKNSNYSINCRVTSNPPPEFIKWYKLSN--MTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKP--KEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWI-------DVETKDIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQG 2264
            +E+ G   C A+N+AG      SL   +++   P IK     +P  V  ++N++  + C    NPA    W   +  QI+   +  KF +  R            G  +C A+N  GS K+   LNV   P + I  N+    V  N   S+NC V+  PPP+ + W K       +++ + +     L  I    S  G Y C+  N         +   S+K + LTV   P  K+       I+++ +G    ++C ++   P    P + W        D    +I++  +T  +  +E    G+Y C A N  G+        V V   P  I   +   +  + S+    ++ TC+  G P     W               +++H+ I N    ++       L   +  Q    + S +G Y C A N  G A  ++ L I+ PP +       +++  L +NV ++ C A+ I T H++W +  + IV                  E ER   T     + L I      D   Y+CVA+N  G
Sbjct: 2839 VEDAGRYTCVAVNEAG----EDSLRYDVHVLLPPVIKGANSDLPEEVTVLVNKSTQMECSSSGNPAPRNYW--QKDGQILLEDEHHKFQSDGRSLQILNAQITDTGRYVCVAENTAGSAKKYFNLNVHVPPSV-IGPNHEHLSVVVNHFISLNCEVSGFPPPD-LSWLKNEEPIKPNTNVLTVPGGRTLQIIRAKISDGGDYTCIAIN---------QAGESKKKVSLTVHVPPSIKDHGSQSLSIVNVREGTSVSLECESNAVPP----PVITWSKNGRMIPDSTNVEILTGGQTLHIRRAEVSDTGQYVCRAINVAGRDDKNFHLNVYV---PPTIEGPETEVIVETISN---PVTLTCDATGIPPPTITW---------------LKNHKPIENSDPLEVHI-----LSGGSKLQIARPQRSNSGNYTCVASNMEGKAQKNFILFIQVPPSVAGAEVPSEVSVLLGENVELV-CNADGIPTPHLQWLRDGKPIV----------------NGETERVRVT--TDGSTLNIYRALTSDMGKYTCVATNPAG 3325          

HSP 6 Score: 90.1225 bits (222), Expect = 1.876e-17
Identity = 162/689 (23.51%), Postives = 254/689 (36.87%), Query Frame = 3
Query:  348 SHESRLTIHNISSDNSGYYVCRVTLLTTSEHIISLAVVTVKTPP--KSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKI--KPFVPNTVNAMINQTIDLSCEVYANPAATIQW-------VIMQGLQIIDSSKFIA--KIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLI---HIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSN---MTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKI---ISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETKDIVSDMETFQVINSESRH--------NGKYKCLAENEIG---KSFSRETALVTVNEAPSI---ITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQP 2306
            S +  L+I      ++G Y C  T +  S+   +   + V+ PP  + +   FN         + I   C   G  P P I         +   + ++   P  +I E  A+ +       N G  +C A+N+AG  T+    L +   P I  K  V N V+ + +Q   L CEV   P+  I W            +QI+++ K +   K+  +  G   C+A N  G++++   +NVL  P I      S   V  N N ++ C  T  P P  I W+K      + D +  L      L+     RS  G Y C   N      GK+      K + LTV Y P  P I G  I   IS +      ++C T     G P P + W   + + + S  +   +   +  H        +  Y C A N  G   KSF      V +   P+I   +T   N++V +      QS+   C+  G P     W    + +P  + D     H     +++ +I + L +D                 G+Y C A +  G   + Y + +  PP ++           L  N++ + CQ        I W K  + I                     ERD F   L   +L I   +  D   Y CVA+N  G  +  FEV     P
Sbjct: 1220 SPDGTLSIEQAVISDAGVYTCAATNIAGSDE--AEVTLHVQEPPSVEDLQPPFNTPFQERLANQRIEFPCPAKG-TPKPTIKW-------LHNGREVTGQEPGVSILEDGALLVIASVTPHNNGEYICVAVNEAGT-TERKYNLKVHVPPVIRDKEHVTN-VSVLTSQLASLYCEVEGTPSPVITWYKDDIQVTESSTVQIVNNGKILKLFKVSAEDAGRYSCKAINIAGTSQKDFSVNVLVPPSILGASSPSEVSVVLNHNVTLQCPGTGVPFPA-IHWFKDGKPLFLGDPNIELSDRGQSLHLRNARRSDKGRYQCTVSN----AAGKQA-----KDIKLTV-YVP--PSIKGGNITTEISALLNSIVKLECETR----GLPVPAITWYK-DGQVVTSSSQALYIDKGQLLHIQRAQVSDSATYTCHAANVAGTAEKSFH-----VDIYVPPTIEGDLTAPSNKQVIIG-----QSLILECKAAGNPPPILTWL--KDGVPVKASD---NIHIEAGGKKL-EILSALEVD----------------RGQYICVATSVAGEREIKYEVDVLVPPAVEGG-EETSYFIVLANNLLELDCQVSGSPPPTIMWLKGGQLI--------------------DERDGFKILLNGRKLVIAQAQVSDTGLYQCVATNIAGDHRKEFEVTVHVPP 1825          

HSP 7 Score: 77.7962 bits (190), Expect = 1.174e-13
Identity = 175/741 (23.62%), Postives = 279/741 (37.65%), Query Frame = 3
Query:  159 PNNATVNSKENLTLEC-VVDQQNIVYSWEYKPFHIKSNIMSKVLGFCYFEKPCVVPDSESQITISHESRLTIHNISSDNSGYYVCRVTLLTTSEHIISLAVV--TVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKI-----SMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQWVIMQGLQIIDSSKFIA--------KIRPDMD---GPLICEAKNYLGSTKETVQLNVLRDPL-IHIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMTDSS---AVLISDDAKLNFILIDR---SSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILG--NKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETKDI------VSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRH----RKIAAS-LNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQG 2264
            PNN TV   E   L C V+   +   +W+     I+                  + DS    T+++ S L + ++   ++G Y C V    +SE   + A V  TV+  PK      N+S   F     I+I CS + G P PKI      M+I+  +   R ++ S      T+   +A+        ++ G   C A N AG   K TS L     PK+       + A+ + T+ + C+    P   ++W   +G   +  S F++        KI+   D   G   C A N  G     + L+V   P+ I   S+  V   SN ++ C V   P P+ IKW +L NM   S   +V      +   + I     S  G Y C  +N      GK +   S+ T+ +T    P    ++G    + S+++G+   + C+      G P P  +W+      +      V    +  +     +  GKY C+A N  G   + +T  V V+  PSI  QH  +++  +   +P  ++  C   G P     W    E I  SS  F      +                LY        S  S  +G Y C A N  G A     L +   P +  + R     + +  S L     ++ C+A+S+    I W K +  ++ PF                  R  F P      + I +    D   Y CVA+N  G
Sbjct:  526 PNNVTVTPGERAVLACLVISAVDYNLTWQRSGRDIR------------------LADSARIRTLANLS-LELRSVKIGDAGEYRCVV----SSEGGSAAASVFLTVQEKPKVTVMPKNQS---FTGGSEISIMCSAT-GYPKPKIVWTMNEMFIMGSH---RYRMTS----EGTLFIKNAVP-------KDAGTYACLASNAAGT-DKQTSTLRYIEAPKLVVEQSELLVALGDTTV-MECKTSGIPPPQVKW--FKGDLELRPSTFLSIDPLVGLLKIQETQDLDAGDYTCVAINEAGRATGRLTLDVGSPPVFIQEPSDVAVEIGSNVTLPCYVQGYPEPK-IKWRRLDNMPVFSRPFSVSFISQLRTGALFISNLWASDKGTYICEAEN----QFGKIQ---SQTTVTVTGLVAP----LIGISPSMASVIEGQPLTLPCTL---LAGNPIPERRWMKNSAMLVQNPYITVRSDGSLHIERVRLQDGGKYTCVASNVAGT--NNKTTSVAVHVLPSI--QH-GQQILSTIEGVP--VTLPCRASGIPKPSITWSKKGELISTSSAKFSAGADGS----------------LYV------VSPGSEESGEYICTATNAAGYAKRKVQLTVYVRPRVFGDQRGLSQDKPVEISVLAGEEAILPCEAKSLPPPIITWAK-DSQLISPFSP----------------RHTFLPS---GSMKITETRVSDSGMYLCVATNIAG 1157          

HSP 8 Score: 77.7962 bits (190), Expect = 1.194e-13
Identity = 145/669 (21.67%), Postives = 246/669 (36.77%), Query Frame = 3
Query:  390 NSGYYVCRVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKR---LKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQWV--------IMQGLQIIDSSKF--IAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLIHIDSNYR---VPKNSNYSINCRVTSNPPPEFIKWYKLSN--MTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNK-IISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETK--------DIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNE---RISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQP 2306
            + G Y+C  T +     I     V V   P ++      S  +      + + C VSG  PPP I M++     I      KI+  N     IA+           + + G   C A N AG   K   +  +   P IK         +  + + L C     P  +I W+            L+I  S +   IAK   +  G   C A N  G   +  QL+V   P +      R   V  N+   + C+ T  P P  I WYK S+     +SA  +     L       S  G+Y CV  N           A + +  +    + P  P I G+  ++ ++      ++C    ++ G P P+L W+   +          I+S      + +++    G+Y C+A N  G+   R+  L  V   P+I+ + +N  V +      Q++   C+              ++ +PP +  + ++D R +       IS+  + L I+            ++   GRY CEA N  G    +Y + +  PP I  +    ++ A +  N++ + C++  I    + W K    +VL   + +  I                       L I   E  D   Y+CVASN  G+ K  + +    +P
Sbjct: 1705 DRGQYICVATSVAGEREIKYEVDVLV---PPAVEGGEETSYFIVLANNLLELDCQVSGS-PPPTI-MWLKGGQLIDERDGFKILL-NGRKLVIAQAQ---------VSDTGLYQCVATNIAGDHRKEFEV-TVHVPPTIKSSDLPEKTVVRYKPVTLQCIANGIPNPSITWLKDDQPVNTAHGNLKIQSSGRVLQIAKALLEDAGRYTCVATNAAGEAHQHTQLHVHEPPSLDDAGKMRNETVVVNNPIQLECKATGKPLP-VITWYKDSHPLSGSASAAFLKRGQVLEIGSAQISDAGIYKCVAIN----------SAGATELFYSLQVHVP--PSISGSSSMVEVVVNNLARLEC----EARGIPAPSLTWLKDGSPVSSFSNGIQILSGGRILALTSAQMSDAGRYTCVAVNAAGEK-QRDIDL-RVYAPPNIMGEEQNVSVLIG-----QAVELFCQ--------------SDAVPPPTLMW-LKDGRPLLKRPGLSISENGSVLKIE----------DAQAGDTGRYTCEATNVAGKTEKNYNVNVWVPPSIYGSDELVQLTA-IEGNLITLLCESSGIPPPDLTWKKKGS-LVLADSAGRVHILSGG-----------------RRLQISIAEKADAGLYTCVASNVAGVAKKEYNLQVYIRP 2289          

HSP 9 Score: 76.6406 bits (187), Expect = 2.442e-13
Identity = 165/743 (22.21%), Postives = 254/743 (34.19%), Query Frame = 3
Query:  147 FLITPNNATVNSKENLTLECVVDQQNIVYSWEYKPFHIKSNIMSKVLGFCYFEKPCVVPDSESQITISHESRLTIHNISSDNSGYYVCRV-TLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQWVIMQGLQIIDSSKFIAK-------IRPDMD--GPLICEAKNYLGSTKETVQLNVLRDPLIHIDS---------NYRVPKNSNYSINCRVTSNPPPEFIKWYK----LSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDV------ETKDIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEV 2288
            F+  P++  V    N+TL C        Y   Y    IK   +  +  F         P S S I+      L I N+ + + G Y+C         +   ++ V  +  P   IS      I    E + + + C++  G P P+          +K   ++  N P  T+    ++ + R   L++ G   C A N AG   K TS+  +   P I+      ++ +    + L C     P  +I W     L    S+KF A        + P  +  G  IC A N  G  K  VQL V   P +  D             V       + C   S PPP  I W K    +S  +     L S   K+    +  S +GMY CV  NI           N  +++ L+V   PK     GN+ I +  G++  I C+      G P P + W           +   S   T  +  +     G Y C A N  G   +  T  + V E PS+                 Q I F C  +G P    +W +   ++        I +   +           L I           S+    NG Y C A N  G     Y L   + P +  +  H    + L   +  + C+ E   +  I WYK  + I +   S    +   ++ K      LF             +  +D   YSC A N  G  + +F V
Sbjct:  795 FIQEPSDVAVEIGSNVTLPC--------YVQGYPEPKIKWRRLDNMPVFSR-------PFSVSFISQLRTGALFISNLWASDKGTYICEAENQFGKIQSQTTVTVTGLVAPLIGISPSMASVI----EGQPLTLPCTLLAGNPIPE-------RRWMKNSAMLVQN-PYITVRSDGSLHIERVR-LQDGGKYTCVASNVAGTNNKTTSVA-VHVLPSIQ-HGQQILSTIEGVPVTLPCRASGIPKPSITWSKKGELISTSSAKFSAGADGSLYVVSPGSEESGEYICTATNAAGYAKRKVQLTVYVRPRVFGDQRGLSQDKPVEISVLAGEEAILPCEAKSLPPP-IITWAKDSQLISPFSPRHTFLPSGSMKITETRV--SDSGMYLCVATNIA---------GNVTQSVKLSVHVPPKIQH--GNRHIKVQVGQRVDILCNAH----GSPPPVITWFKSGRPFLDGAQHPGSPDGTLSIEQAVISDAGVYTCAATNIAGSDEAEVT--LHVQEPPSVEDLQPPFNTPFQERLANQRIEFPCPAKGTPKPTIKWLHNGREVTGQEPGVSILEDGAL-----------LVI----------ASVTPHNNGEYICVAVNEAGTTERKYNL-KVHVPPVIRDKEHVTNVSVLTSQLASLYCEVEGTPSPVITWYK--DDIQVTESSTVQIVNNGKILK------LF------------KVSAEDAGRYSCKAINIAGTSQKDFSV 1445          

HSP 10 Score: 75.485 bits (184), Expect = 5.254e-13
Identity = 184/829 (22.20%), Postives = 302/829 (36.43%), Query Frame = 3
Query:   21 GTLSTDNETYNLLSNR*-----NMEYFKTFILITLFGNLMD---RSYTLNFLITPNNATVNSK---ENLTLECVVDQQNIVYSWEYKPFHIKSNIMSKVLGFCYFEKPCVVPDSESQITISHESR-LTIHNISSDNSGYYVCRVTLLTTSEHIISLAVVTVKTP-------------PKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPF--VPNTVNA---------MINQTIDLSCEVYANPAATIQW-------------VIMQGLQIIDSSKFIAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLIHIDSNYRVPK------NSNYSINCRVTSNPPPEFIKWYKLSN--MTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILG--NKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETKD-------IVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEP-ERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQP 2306
            G L  ++E ++++S        N +   T     L  N+     +S+ LN  ++P  A V+S    E++ +        +  ++ Y P  I            +F+    + +S   I I    R L I N   DN+G Y C V      E I    V     P             PK +    N S+ +  E E  AI          P  S+   KD   + LK    +  + TIA        +   + + G   C A N AG    +  + NI+  P  +    + N ++          +IN  + L CE  A P  T+ W             +I+ G +++     I + + +  G   C A N  G       ++VL  P+I   +N  +P+      N +  + C  + NP P    W K     + D      SD   L  +    + TG Y CV +N           A S K  F    + P  P ++G  ++ +S++      + C       G P P L W+  E           V    T Q+I ++    G Y C+A N+ G+S  ++   +TV+  PSI   H ++ + +       S+S  CE    P     W   N ++ P S + +I       + R +++                     S  G+Y C A N  G    ++ L +  PP I+      ++      N V + C A  I    I W K ++ I                   +P E  + + G K   L I   +  +  NY+CVASN +G  + NF +  +  P
Sbjct: 2516 GQLLQEDEAHHMMSGGRFLQITNAQVSHTGRYTCLASNIAGDKSKSFRLNVFVSPTIAGVDSDGSPEDVIVILNSPTSLVCEAYSYPPATIT-----------WFKDGTPL-ESNRNIRILPGGRTLQILNAQEDNAGRYSC-VATNEAGEKIKHYEVKVYIPPIIKKGDLLGPGLSPKEVKIRVNSSLTL--ECEAYAI----------PSASLRWYKDG--QPLK----SDDHVTIAASGHTLQIKEAQISDTGRYTCVASNLAGEDELDFDV-NIQVPPSFQKLWEIGNMLDTGRSGEAKDVIINNPLSLHCETNAAPPPTLTWYKDGRPLTSSDRVLILPGGRVLQ----IPRAKVEDAGRYTCVAVNEAGEDSLRYDVHVLLPPVIK-GANSDLPEEVTVLVNKSTQMECSSSGNPAPRNY-WQKDGQILLEDEHHKFQSDGRSLQILNAQITDTGRYVCVAENT----------AGSAKKYFNLNVHVP--PSVIGPNHEHLSVVVNHFISLNCEVS----GFPPPDLSWLKNEEPIKPNTNVLTVPGGRTLQIIRAKISDGGDYTCIAINQAGES--KKKVSLTVHVPPSI-KDHGSQSLSIVNVREGTSVSLECESNAVPPPVITWSK-NGRMIPDSTNVEILTGGQTLHIRRAEV---------------------SDTGQYVCRAINVAGRDDKNFHLNVYVPPTIEGP--ETEVIVETISNPVTLTCDATGIPPPTITWLKNHKPI----------------ENSDPLEVHILSGGSK---LQIARPQRSNSGNYTCVASNMEGKAQKNFILFIQVPP 3244          

HSP 11 Score: 71.633 bits (174), Expect = 8.170e-12
Identity = 100/441 (22.68%), Postives = 173/441 (39.23%), Query Frame = 3
Query:  345 ISHESRLTIHNISSDNSGYYVCRVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQW-----VIMQGLQIID--SSKFIAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLIHID--SNYRVPKNSNYSINCRVTSNPPPEFIKW-YKLSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIM-KGEKFIIKCSTDRKSPGEPFPTLKW------IDVETKDIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSI 1616
            I +   L I     D++G Y C    +  +      + +TV  PP+  S   + ++N   E     + C V+ GIP P I  +  KD       ++ +N      A+     +     LE+ G   C A N AG  T+  +L  +   P      P  ++    + + LSC+    P   + W     +I      I+  S   I K+  +  G  +C A+N +G  K    + V   P+   D  SN+  P   N  +NC V  +P P  I+W  K +++  S  +    +  L          G Y CV  N         +    E++M LT+      P I    + +++  G + I+ C    ++ GEP PT+ W      I  + +  +    +  +  +      +Y+C+A N +G    R   +V V+   S+
Sbjct: 4126 ILNSGALQIAFAQPDDAGQYTCMAANM--AGSSSVSSTLTVHVPPRIQSTEVHFTVN---ENSQAVLPC-VADGIPTPAI--HWEKDG------VLIANLLGKYTAQPYGELILENVVLEDSGTYTCVANNAAGEDTRIVTLA-VHTLPTFTEL-PGDLSLNKGEQLRLSCKAVGIPLPKLTWTFNNNIIPAHFDSINGHSELVIEKVSKEDSGTYVCTAENSVGFVKAIGFVYVKEPPVFKGDYPSNWIEPLGGNAILNCEVKGDPAPT-IQWSRKGADIEISHRIRQLGNGSLAIYGTVNEDAGDYTCVAAN---------EAGMVERSMSLTLQ---SSPIITLEPVETVVDAGGRVILDC----QAAGEPQPTITWSRQGQPISWDNRLSMLPNSSLYIAAARKEDTSEYECVARNLMGSVLVRVPVIVQVHGGFSL 4533          

HSP 12 Score: 67.0106 bits (162), Expect = 2.195e-10
Identity = 93/431 (21.58%), Postives = 171/431 (39.68%), Query Frame = 3
Query:  699 LENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQWV-IMQGLQIIDSSKF--IAKIRPDMDGPLICEAKNYL-------GSTKETVQLNVLRDPLIHIDSNYRVPKNSNY------SINCRVTSNPPPEFIKWYKLSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWI--DVETK-----DIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHG 1922
            L + GF  C A++ AG   +  +  ++   P I    PN V     +   L+C V +     + W    + +++ DS++   +A +  ++    I +A  Y        GS   +V L V   P + +     +PKN ++      SI C  T  P P+ +       +  S    ++ +  L          G YAC+  N              ++T  L     PK   ++    + +  G+  +++C    K+ G P P +KW   D+E +      I   +   ++  ++    G Y C+A NE G++  R T  + V   P  I +  +  V     ++  +++  C  +G P  + +WR   + +P  S+ F +     IS  R   +F               ++L +S  G Y CEA+N+ G
Sbjct:  491 LSDEGFYDCIAVSSAGT-GRAQTFFDVSEPPPIIQL-PNNVTVTPGERAVLACLVISAVDYNLTWQRSGRDIRLADSARIRTLANLSLELRSVKIGDAGEYRCVVSSEGGSAAASVFLTVQEKPKVTV-----MPKNQSFTGGSEISIMCSATGYPKPKIVWTMNEMFIMGSHRYRMTSEGTLFIKNAVPKDAGTYACLASNAA---------GTDKQTSTLRYIEAPK--LVVEQSELLVALGDTTVMEC----KTSGIPPPQVKWFKGDLELRPSTFLSIDPLVGLLKIQETQDLDAGDYTCVAINEAGRATGRLT--LDVGSPPVFIQEPSDVAV-----EIGSNVTLPCYVQGYPEPKIKWRR-LDNMPVFSRPFSVS---FISQLRTGALFI--------------SNLWASDKGTYICEAENQFG 874          

HSP 13 Score: 55.8398 bits (133), Expect = 5.491e-7
Identity = 84/365 (23.01%), Postives = 143/365 (39.18%), Query Frame = 3
Query:  702 ENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPF-VPNTVNAMINQTIDLSCEVYANPAATIQW-------VIMQGLQIIDSSKF--IAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDP-LIHID--SNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMTDSS--AVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKW-----IDVETKDIVSDMETFQVINSESRH---NGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWR 1727
            E+ G   C A N+AG ++K+  +L +   P I     P  V+ ++N  ++LSC     PA  I W       +  + +Q ++      ++  + +  G   C A +  G   +   + V   P +  +D   ++ V +N   ++ C+  + PPP  I W K      ++    ++S    L     D   T  Y CV  NI   TT         +   LTV   P      G + +  +  +   ++C    ++ G P P + W     +  E+    S +E    ++ ES H    G+Y C+A N  G    R    + V+  PSI     N  V ++        +  CE  G P     WR
Sbjct: 3498 EDTGKYTCVATNEAGEVSKHF-VLKVLEPPHINGSEGPGEVSVIVNNPLELSCIASGIPAPKISWMKDGRPFLQTEQVQTLEGGAILRVSSAQVEDTGRYTCLASSPAGDDDKEYLVRVHVPPNIAGMDEAQDFTVLRNRQVTLECKSDAVPPP-VIMWLKNREQLQATPRVRILSGGRYLQINNADLGDTANYTCVASNIAGKTT---------REFNLTVNVPPSIGG--GPQSLVTLLNKSIALEC----RAEGVPAPRITWRKDGVVLAESHARYSILEN-GFLHIESAHVTDTGRYLCMATNVAGT--DRRRIDLQVHVPPSIAMGPTNVTVTVN-----VQTTLACEATGIPKPSVTWR 3837          
BLAST of hemicentin-1 isoform X2 vs. Ensembl Mouse
Match: Hmcn1 (hemicentin 1 [Source:MGI Symbol;Acc:MGI:2685047])

HSP 1 Score: 102.834 bits (255), Expect = 2.293e-21
Identity = 168/689 (24.38%), Postives = 265/689 (38.46%), Query Frame = 3
Query:  363 LTIHNISSDNSGYYVCRVTLLTTSEHIISLAVVTVKTPPKSIS-FHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSA---ISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNT---VNAMINQTIDLSCEVYANPAATIQWVIMQGLQIIDSSKF----------IAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLIHI-DSNYRVPKNSN-YSINCRVTSNPPPEFIKWYK----LSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKI---ISIMKGEKFIIKCSTDRKSPGEPFPTLKWI---------DVETKDIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVN--EAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQPDPPY 2318
            L I N    ++G YVC      T+        + V  PP  I   H + S+ V     FI++ C VS G PPP +S ++  +  IK         PN+ +  V     + + R   + + G   C AIN AG   K  S L +   P IK     +   VN     ++ L CE  A P   I W    G  I DS+            I +      G  +C A N  G   +   LNV   P I   ++   V   SN  ++ C  T  PPP  I W K    + N       ++S  +KL      RS++G Y CV  N++    GK   A     +F+ V      P + G ++   +S++ GE   + C+ D    G P P L+W+         + E   + +D  T  +  + +   GKY C+A N  G+    E  +  +N    P I    +  +  ++  D   SI+  C+  G P  +  W      +P SS        R +S  ++ +I      D+                  Y C A NR G+ S  Y L +  PP +   +   +I      +  M  C  +      + W +  + +            +A +T       + T G+    L +   E +D   Y+CVA+N+ G  +V+   V K   +PP+
Sbjct: 2927 LQILNAQITDTGRYVCVAE--NTAGSAKKYFNLNVHVPPSVIGPNHEHLSVVV---NHFISLNCEVS-GFPPPDLS-WLKNEEPIK---------PNTNVLTVPGGRTLQIIRAK-ISDGGDYTCIAINQAGESKKKVS-LTVHVPPSIKDHGSQSLSIVNVREGTSVSLECESNAVPPPVITWS-KNGRMIPDSTNVEILTGGQTLHIRRAEVSDTGQYVCRAINVAGRDDKNFHLNVYVPPTIEGPETEVIVETISNPVTLTCDATGIPPPT-ITWLKNHKPIENSDPLEVHILSGGSKLQIARPQRSNSGNYTCVASNME----GK---AQKNFILFIQV-----PPSVAGAEVPSEVSVLLGENVELVCNAD----GIPTPHLQWLRDGKPIVNGETERVRVTTDGSTLNIYRALTSDMGKYTCVATNPAGE----EDRIFNLNVYVPPKIRGNKEEAEKLMALVD--TSINIECKATGTPPPQINWLKNGLPLPISSH------IRLLSAGQVVRIVRAQVSDI----------------AVYTCVASNRAGVDSKHYSLQVFVPPNMDNAMGTEEITIVKGSSTSMT-CFTDGTPAPSMSWLRDGQPLAP----------DAHLT-------VSTQGMV---LQLIKAETEDTGKYTCVATNEAG--EVSKHFVLKVL-EPPH 3527          

HSP 2 Score: 102.834 bits (255), Expect = 2.669e-21
Identity = 154/697 (22.09%), Postives = 258/697 (37.02%), Query Frame = 3
Query:  315 VVPDSESQITISHESRLTIHNISSDNSGYYVCRVTLLTTSE----HIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNST--IAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPF-VPNTVNAMINQTIDLSCEVYANPAATIQWVIMQGLQIIDSSKFIAKIRPDMD------------GPLICEAKNYLGSTKETVQLNVLRDPLIHID----SNYRVPKNSNYSINCRVTSNPPPEFIKWYK--LSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETKDIVSDME--------TFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQP 2306
            ++PDS +   ++    L I      ++G YVCR   +   +    H+      T++ P           + V      + + C  +G IPPP I+        +K  K I ++ P     ++  S + + RP    N G   C A N  G   KN  +L I+  P +    VP+ V+ ++ + ++L C     P   +QW +  G  I++      ++  D              G   C A N  G       LNV   P I  +           +++ +I C+ T  PPP+ I W K  L     S   L+S    +  +    S   +Y CV  N         +     K   L V   P     +G + I+I+KG    + C TD    G P P++ W+  + + +  D            Q+I +E+   GKY C+A NE G+  S+   L  V E P I       +V +  ++    +  +C   G P  +  W               ++D R         + T+    L      + +S +    GRY C A +  G    +Y + +  PP I      +      N+ V +  C+++++    I W K  E +                 +  P   + + G     L I + +  D +NY+CVASN  G     F +     P
Sbjct: 3098 MIPDSTNVEILTGGQTLHIRRAEVSDTGQYVCRAINVAGRDDKNFHLNVYVPPTIEGP--------ETEVIVETISNPVTLTCDATG-IPPPTITW-------LKNHKPIENSDPLEVHILSGGSKLQIARPQR-SNSGNYTCVASNMEGKAQKNF-ILFIQVPPSVAGAEVPSEVSVLLGENVELVCNADGIPTPHLQW-LRDGKPIVNGETERVRVTTDGSTLNIYRALTSDMGKYTCVATNPAGEEDRIFNLNVYVPPKIRGNKEEAEKLMALVDTSINIECKATGTPPPQ-INWLKNGLPLPISSHIRLLSAGQVVRIVRAQVSDIAVYTCVASN---------RAGVDSKHYSLQVFVPPNMDNAMGTEEITIVKGSSTSMTCFTD----GTPAPSMSWLR-DGQPLAPDAHLTVSTQGMVLQLIKAETEDTGKYTCVATNEAGE-VSKHFVL-KVLEPPHINGSEGPGEVSVIVNN---PLELSCIASGIPAPKISW---------------MKDGRPF-------LQTEQVQTLEGGAILRVSSAQVEDTGRYTCLASSPAGDDDKEYLVRVHVPPNIAGMDEAQDFTVLRNRQVTL-ECKSDAVPPPVIMWLKNREQL-----------------QATPRVRILSGG---RYLQINNADLGDTANYTCVASNIAGKTTREFNLTVNVPP 3712          

HSP 3 Score: 98.9821 bits (245), Expect = 3.460e-20
Identity = 151/698 (21.63%), Postives = 266/698 (38.11%), Query Frame = 3
Query:  315 VVPDSESQITI-SHESRLTIHNISSDNSGYYVC---RVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKI--KPFVPNTVNAMINQTIDLSCEVYANPAATIQWV-------IMQGLQIIDSSKFI--AKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLIHIDS---NYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNM--TDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETK-------DIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLP----QSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDY---------FLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEV 2288
            V+ DS  ++ I S   RL I      ++G Y C    V  +   E+ + + +     P  + S      I V + +  I+++C V G IP P ++ ++     + + K +        I +   I   +  ++ + G  +C A+N AG+  K   L ++   P I     VP  V+ +   ++ L+CE    P  +I W+       +   ++I+   + +   + RP+  G   C  +N  G  ++   L+VL  P I  ++   + ++ +  + ++ C V  NP P+ I W+K   +   D +  ++S    L       S TG Y C+  NI        K  +    +F++ T    +       +I I+     ++ C      P     T+ W    T         I+    T Q++N++  + G+Y C+A NE G+        V +   P II +       LS  ++      S++  CE    P    RW           Q  K +DH              +TI     T  Q    + S  GRY C A N  G   LD+         F  +     +    R  +    +  N + + C+  +     + WYK       P  S               +R L  PG +V  L I   + +D   Y+CVA N+ G   + ++V
Sbjct: 2236 VLADSAGRVHILSGGRRLQISIAEKADAGLYTCVASNVAGVAKKEYNLQVYI----RPSITNSGGHRPEITVIRGKS-ISLECEVQG-IPQPTVT-WMKDGRPLTKGKGVE-------ILDEGRILQLKNVHVSDTGRYVCVAVNVAGMTDKRYDL-SVHAPPSIIGNHGVPENVSVVEKSSVSLTCEASGIPLPSITWLKDGWPVNLGSSVKILSGGRMLRLMQTRPEDAGQYTCIVRNAAGEDRKMFGLSVLVPPHIVGENTLEDVKIKEKQSVTLTCEVRGNPVPQ-ITWHKDGQLLQEDEAHHMMSGGRFLQITNAQVSHTGRYTCLASNI-----AGDKSKSFRLNVFVSPTIAGVDSDGSPEDVIVILNSPTSLV-CEAYSYPPA----TITWFKDGTPLESNRNIRILPGGRTLQILNAQEDNAGRYSCVATNEAGEKIKHYEVKVYI---PPIIKKGDLLGPGLSPKEVKIRVNSSLTLECEAYAIPSASLRWYK-------DGQPLKSDDH--------------VTIAASGHTL-QIKEAQISDTGRYTCVASNLAGEDELDFDVNIQVPPSFQKLWEIGNMLDTGRSGEAKDVIINNPLSLHCETNAAPPPTLTWYKDGR----PLTSS--------------DRVLILPGGRV--LQIPRAKVEDAGRYTCVAVNEAGEDSLRYDV 2862          

HSP 4 Score: 93.2041 bits (230), Expect = 2.186e-18
Identity = 152/679 (22.39%), Postives = 265/679 (39.03%), Query Frame = 3
Query:  363 LTIHNISSDNSGYYVCRVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRL------KIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQWVIMQGLQIIDSSKFIA-----------KIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLIHI-DSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSN-MTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKI-ISIMKGEKFIIKCSTDRKSPGEPFPTLKW-------IDVETKDIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRET----ALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQP 2306
            L I + S D++  Y C VT  + +        +TV+ PP +I+   ++ ++    ++  A+    + G+P P I       N ++ L      +I+SS           AI +     L + G   C A N AG   ++ +L  ++  P I+P  P+ ++ ++N  I L CE    P   I W   +G+ +I S K +A            +R D  G  +C A+N  G+    V+LNV   P+I      Y V  +   S+ C    +PPP+ I W+K  + +T+S    I +   L          G Y C+  N+           +S  +  LTV   P+   I   ++  ++ +  + ++ C  D    G P P + W        ++  K          + N     +G Y C+A N  G+     T     L T  E P  ++ +K  ++ LS           C+  G PL +  W + N  IP  +    I  H  +  E++S+                        +G Y C A+N  G      F+ +K PP+ K +     I   L  N ++  C+ +      I+W +    I +  + ++                     L    L I    N+D  +Y+CVA+N+ G+ + +  +  +S P
Sbjct: 3860 LVIISPSVDDTASYECTVT--SDAGEDKRAVDLTVQVPP-TIA---DEPMDFLVTRQAPAVMTCSASGVPVPSIHW---TKNGLRLLPRGDGYRILSSG----------AIEIPTTQ-LNHAGRYTCVARNAAGSAHRHVTL-RVQEPPVIQP-QPSELDVILNNPILLPCEATGIPTPFITWQ-KEGINVITSGKSLAILPSGSLQISRAVRGDA-GTYMCVAQNPAGTALGKVKLNVQVPPVISSHQKEYVVTMDKPVSLLCETEGSPPPD-ITWHKDGHALTESIRQRILNSGALQIAFAQPDDAGQYTCMAANMA---------GSSSVSSTLTVHVPPR---IQSTEVHFTVNENSQAVLPCVAD----GIPTPAIHWEKDGVLIANLLGKYTAQPYGELILENVVLEDSGTYTCVANNAAGEDTRIVTLAVHTLPTFTELPGDLSLNKGEQLRLS-----------CKAVGIPLPKLTWTFNNNIIP--AHFDSINGHSELVIEKVSK----------------------EDSGTYVCTAENSVGFVKAIGFVYVKEPPVFKGDYPSNWIEP-LGGNAIL-NCEVKGDPAPTIQWSRKGADIEISHRIRQ---------------------LGNGSLAIYGTVNEDAGDYTCVAANEAGMVERSMSLTLQSSP 4439          

HSP 5 Score: 90.5077 bits (223), Expect = 1.665e-17
Identity = 130/553 (23.51%), Postives = 217/553 (39.24%), Query Frame = 3
Query:  699 LENRGFVMCTAINDAGVLTKNTSL---LNIKYKPKIKPF---VPNTVNAMINQTIDLSCEVYANPAATIQWVIMQGLQII---DSSKFIAKIRPDM--------DGPLICEAKNYLGSTKETVQLNVLRDPLIHIDSNYR---VPKNSNYSINCRVTSNPPPEFIKWYKLSN--MTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKP--KEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWI-------DVETKDIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQG 2264
            +E+ G   C A+N+AG      SL   +++   P IK     +P  V  ++N++  + C    NPA    W   +  QI+   +  KF +  R            G  +C A+N  GS K+   LNV   P + I  N+    V  N   S+NC V+  PPP+ + W K       +++ + +     L  I    S  G Y C+  N         +   S+K + LTV   P  K+       I+++ +G    ++C ++   P    P + W        D    +I++  +T  +  +E    G+Y C A N  G+        V V   P  I   +   +  + S+    ++ TC+  G P     W               +++H+ I N    ++       L   +  Q    + S +G Y C A N  G A  ++ L I+ PP +       +++  L +NV ++ C A+ I T H++W +  + IV                  E ER   T     + L I      D   Y+CVA+N  G
Sbjct: 2839 VEDAGRYTCVAVNEAG----EDSLRYDVHVLLPPVIKGANSDLPEEVTVLVNKSTQMECSSSGNPAPRNYW--QKDGQILLEDEHHKFQSDGRSLQILNAQITDTGRYVCVAENTAGSAKKYFNLNVHVPPSV-IGPNHEHLSVVVNHFISLNCEVSGFPPPD-LSWLKNEEPIKPNTNVLTVPGGRTLQIIRAKISDGGDYTCIAIN---------QAGESKKKVSLTVHVPPSIKDHGSQSLSIVNVREGTSVSLECESNAVPP----PVITWSKNGRMIPDSTNVEILTGGQTLHIRRAEVSDTGQYVCRAINVAGRDDKNFHLNVYV---PPTIEGPETEVIVETISN---PVTLTCDATGIPPPTITW---------------LKNHKPIENSDPLEVHI-----LSGGSKLQIARPQRSNSGNYTCVASNMEGKAQKNFILFIQVPPSVAGAEVPSEVSVLLGENVELV-CNADGIPTPHLQWLRDGKPIV----------------NGETERVRVT--TDGSTLNIYRALTSDMGKYTCVATNPAG 3325          

HSP 6 Score: 89.7373 bits (221), Expect = 2.293e-17
Identity = 162/689 (23.51%), Postives = 254/689 (36.87%), Query Frame = 3
Query:  348 SHESRLTIHNISSDNSGYYVCRVTLLTTSEHIISLAVVTVKTPP--KSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKI--KPFVPNTVNAMINQTIDLSCEVYANPAATIQW-------VIMQGLQIIDSSKFIA--KIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLI---HIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSN---MTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKI---ISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETKDIVSDMETFQVINSESRH--------NGKYKCLAENEIG---KSFSRETALVTVNEAPSI---ITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQP 2306
            S +  L+I      ++G Y C  T +  S+   +   + V+ PP  + +   FN         + I   C   G  P P I         +   + ++   P  +I E  A+ +       N G  +C A+N+AG  T+    L +   P I  K  V N V+ + +Q   L CEV   P+  I W            +QI+++ K +   K+  +  G   C+A N  G++++   +NVL  P I      S   V  N N ++ C  T  P P  I W+K      + D +  L      L+     RS  G Y C   N      GK+      K + LTV Y P  P I G  I   IS +      ++C T     G P P + W   + + + S  +   +   +  H        +  Y C A N  G   KSF      V +   P+I   +T   N++V +      QS+   C+  G P     W    + +P  + D     H     +++ +I + L +D                 G+Y C A +  G   + Y + +  PP ++           L  N++ + CQ        I W K  + I                     ERD F   L   +L I   +  D   Y CVA+N  G  +  FEV     P
Sbjct: 1220 SPDGTLSIEQAVISDAGVYTCAATNIAGSDE--AEVTLHVQEPPSVEDLQPPFNTPFQERLANQRIEFPCPAKG-TPKPTIKW-------LHNGREVTGQEPGVSILEDGALLVIASVTPHNNGEYICVAVNEAGT-TERKYNLKVHVPPVIRDKEHVTN-VSVLTSQLASLYCEVEGTPSPVITWYKDDIQVTESSTVQIVNNGKILKLFKVSAEDAGRYSCKAINIAGTSQKDFSVNVLVPPSILGASSPSEVSVVLNHNVTLQCPGTGVPFPA-IHWFKDGKPLFLGDPNIELSDRGQSLHLRNARRSDKGRYQCTVSN----AAGKQA-----KDIKLTV-YVP--PSIKGGNITTEISALLNSIVKLECETR----GLPVPAITWYK-DGQVVTSSSQALYIDKGQLLHIQRAQVSDSATYTCHAANVAGTAEKSFH-----VDIYVPPTIEGDLTAPSNKQVIIG-----QSLILECKAAGNPPPILTWL--KDGVPVKASD---NIHIEAGGKKL-EILSALEVD----------------RGQYICVATSVAGEREIKYEVDVLVPPAVEGG-EETSYFIVLANNLLELDCQVSGSPPPTIMWLKGGQLI--------------------DERDGFKILLNGRKLVIAQAQVSDTGLYQCVATNIAGDHRKEFEVTVHVPP 1825          

HSP 7 Score: 77.411 bits (189), Expect = 1.342e-13
Identity = 171/739 (23.14%), Postives = 277/739 (37.48%), Query Frame = 3
Query:  159 PNNATVNSKENLTLEC-VVDQQNIVYSWEYKPFHIKSNIMSKVLGFCYFEKPCVVPDSESQITISHESRLTIHNISSDNSGYYVCRVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKI-----SMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQWVIMQGLQIIDSSKFIA--------KIRPDMD---GPLICEAKNYLGSTKETVQLNVLRDPL-IHIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMTDSS---AVLISDDAKLNFILIDR---SSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILG--NKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETKDI------VSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRH----RKIAAS-LNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQG 2264
            PNN TV   E   L C V+   +   +W+     I+                  + DS    T+++ S L + ++   ++G Y C V+  +      +   +TV+  PK      N+S   F     I+I CS + G P PKI      M+I+  +   R ++ S      T+   +A+        ++ G   C A N AG   K TS L     PK+       + A+ + T+ + C+    P   ++W   +G   +  S F++        KI+   D   G   C A N  G     + L+V   P+ I   S+  V   SN ++ C V   P P+ IKW +L NM   S   +V      +   + I     S  G Y C  +N      GK +   S+ T+ +T    P    ++G    + S+++G+   + C+      G P P  +W+      +      V    +  +     +  GKY C+A N  G   + +T  V V+  PSI  QH  +++  +   +P  ++  C   G P     W    E I  SS  F      +                LY        S  S  +G Y C A N  G A     L +   P +  + R     + +  S L     ++ C+A+S+    I W K +  ++ PF                  R  F P      + I +    D   Y CVA+N  G
Sbjct:  526 PNNVTVTPGERAVLACLVISAVDYNLTWQRSGRDIR------------------LADSARIRTLANLS-LELRSVKIGDAGEYRCVVS--SEGGSAAASVFLTVQEKPKVTVMPKNQS---FTGGSEISIMCSAT-GYPKPKIVWTMNEMFIMGSH---RYRMTS----EGTLFIKNAVP-------KDAGTYACLASNAAGT-DKQTSTLRYIEAPKLVVEQSELLVALGDTTV-MECKTSGIPPPQVKW--FKGDLELRPSTFLSIDPLVGLLKIQETQDLDAGDYTCVAINEAGRATGRLTLDVGSPPVFIQEPSDVAVEIGSNVTLPCYVQGYPEPK-IKWRRLDNMPVFSRPFSVSFISQLRTGALFISNLWASDKGTYICEAEN----QFGKIQ---SQTTVTVTGLVAP----LIGISPSMASVIEGQPLTLPCTL---LAGNPIPERRWMKNSAMLVQNPYITVRSDGSLHIERVRLQDGGKYTCVASNVAGT--NNKTTSVAVHVLPSI--QH-GQQILSTIEGVP--VTLPCRASGIPKPSITWSKKGELISTSSAKFSAGADGS----------------LYV------VSPGSEESGEYICTATNAAGYAKRKVQLTVYVRPRVFGDQRGLSQDKPVEISVLAGEEAILPCEAKSLPPPIITWAK-DSQLISPFSP----------------RHTFLPS---GSMKITETRVSDSGMYLCVATNIAG 1157          

HSP 8 Score: 77.411 bits (189), Expect = 1.522e-13
Identity = 145/669 (21.67%), Postives = 246/669 (36.77%), Query Frame = 3
Query:  390 NSGYYVCRVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKR---LKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQWV--------IMQGLQIIDSSKF--IAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLIHIDSNYR---VPKNSNYSINCRVTSNPPPEFIKWYKLSN--MTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNK-IISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETK--------DIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNE---RISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQP 2306
            + G Y+C  T +     I     V V   P ++      S  +      + + C VSG  PPP I M++     I      KI+  N     IA+           + + G   C A N AG   K   +  +   P IK         +  + + L C     P  +I W+            L+I  S +   IAK   +  G   C A N  G   +  QL+V   P +      R   V  N+   + C+ T  P P  I WYK S+     +SA  +     L       S  G+Y CV  N           A + +  +    + P  P I G+  ++ ++      ++C    ++ G P P+L W+   +          I+S      + +++    G+Y C+A N  G+   R+  L  V   P+I+ + +N  V +      Q++   C+              ++ +PP +  + ++D R +       IS+  + L I+            ++   GRY CEA N  G    +Y + +  PP I  +    ++ A +  N++ + C++  I    + W K    +VL   + +  I                       L I   E  D   Y+CVASN  G+ K  + +    +P
Sbjct: 1705 DRGQYICVATSVAGEREIKYEVDVLV---PPAVEGGEETSYFIVLANNLLELDCQVSGS-PPPTI-MWLKGGQLIDERDGFKILL-NGRKLVIAQAQ---------VSDTGLYQCVATNIAGDHRKEFEV-TVHVPPTIKSSDLPEKTVVRYKPVTLQCIANGIPNPSITWLKDDQPVNTAHGNLKIQSSGRVLQIAKALLEDAGRYTCVATNAAGEAHQHTQLHVHEPPSLDDAGKMRNETVVVNNPIQLECKATGKPLP-VITWYKDSHPLSGSASAAFLKRGQVLEIGSAQISDAGIYKCVAIN----------SAGATELFYSLQVHVP--PSISGSSSMVEVVVNNLARLEC----EARGIPAPSLTWLKDGSPVSSFSNGIQILSGGRILALTSAQMSDAGRYTCVAVNAAGEK-QRDIDL-RVYAPPNIMGEEQNVSVLIG-----QAVELFCQ--------------SDAVPPPTLMW-LKDGRPLLKRPGLSISENGSVLKIE----------DAQAGDTGRYTCEATNVAGKTEKNYNVNVWVPPSIYGSDELVQLTA-IEGNLITLLCESSGIPPPDLTWKKKGS-LVLADSAGRVHILSGG-----------------RRLQISIAEKADAGLYTCVASNVAGVAKKEYNLQVYIRP 2289          

HSP 9 Score: 76.2554 bits (186), Expect = 2.886e-13
Identity = 165/743 (22.21%), Postives = 254/743 (34.19%), Query Frame = 3
Query:  147 FLITPNNATVNSKENLTLECVVDQQNIVYSWEYKPFHIKSNIMSKVLGFCYFEKPCVVPDSESQITISHESRLTIHNISSDNSGYYVCRV-TLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQWVIMQGLQIIDSSKFIAK-------IRPDMD--GPLICEAKNYLGSTKETVQLNVLRDPLIHIDS---------NYRVPKNSNYSINCRVTSNPPPEFIKWYK----LSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDV------ETKDIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEV 2288
            F+  P++  V    N+TL C        Y   Y    IK   +  +  F         P S S I+      L I N+ + + G Y+C         +   ++ V  +  P   IS      I    E + + + C++  G P P+          +K   ++  N P  T+    ++ + R   L++ G   C A N AG   K TS+  +   P I+      ++ +    + L C     P  +I W     L    S+KF A        + P  +  G  IC A N  G  K  VQL V   P +  D             V       + C   S PPP  I W K    +S  +     L S   K+    +  S +GMY CV  NI           N  +++ L+V   PK     GN+ I +  G++  I C+      G P P + W           +   S   T  +  +     G Y C A N  G   +  T  + V E PS+                 Q I F C  +G P    +W +   ++        I +   +           L I           S+    NG Y C A N  G     Y L   + P +  +  H    + L   +  + C+ E   +  I WYK  + I +   S    +   ++ K      LF             +  +D   YSC A N  G  + +F V
Sbjct:  795 FIQEPSDVAVEIGSNVTLPC--------YVQGYPEPKIKWRRLDNMPVFSR-------PFSVSFISQLRTGALFISNLWASDKGTYICEAENQFGKIQSQTTVTVTGLVAPLIGISPSMASVI----EGQPLTLPCTLLAGNPIPE-------RRWMKNSAMLVQN-PYITVRSDGSLHIERVR-LQDGGKYTCVASNVAGTNNKTTSVA-VHVLPSIQ-HGQQILSTIEGVPVTLPCRASGIPKPSITWSKKGELISTSSAKFSAGADGSLYVVSPGSEESGEYICTATNAAGYAKRKVQLTVYVRPRVFGDQRGLSQDKPVEISVLAGEEAILPCEAKSLPPP-IITWAKDSQLISPFSPRHTFLPSGSMKITETRV--SDSGMYLCVATNIA---------GNVTQSVKLSVHVPPKIQH--GNRHIKVQVGQRVDILCNAH----GSPPPVITWFKSGRPFLDGAQHPGSPDGTLSIEQAVISDAGVYTCAATNIAGSDEAEVT--LHVQEPPSVEDLQPPFNTPFQERLANQRIEFPCPAKGTPKPTIKWLHNGREVTGQEPGVSILEDGAL-----------LVI----------ASVTPHNNGEYICVAVNEAGTTERKYNL-KVHVPPVIRDKEHVTNVSVLTSQLASLYCEVEGTPSPVITWYK--DDIQVTESSTVQIVNNGKILK------LF------------KVSAEDAGRYSCKAINIAGTSQKDFSV 1445          

HSP 10 Score: 75.0998 bits (183), Expect = 6.531e-13
Identity = 120/526 (22.81%), Postives = 198/526 (37.64%), Query Frame = 3
Query:  822 NAMINQTIDLSCEVYANPAATIQW-------------VIMQGLQIIDSSKFIAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLIHIDSNYRVPK------NSNYSINCRVTSNPPPEFIKWYKLSN--MTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILG--NKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETKD-------IVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEP-ERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQP 2306
            + +IN  + L CE  A P  T+ W             +I+ G +++     I + + +  G   C A N  G       ++VL  P+I   +N  +P+      N +  + C  + NP P    W K     + D      SD   L  +    + TG Y CV +N           A S K  F    + P  P ++G  ++ +S++      + C       G P P L W+  E           V    T Q+I ++    G Y C+A N+ G+S  ++   +TV+  PSI   H ++ + +       S+S  CE    P     W   N ++ P S + +I       + R +++                     S  G+Y C A N  G    ++ L +  PP I+      ++      N V + C A  I    I W K ++ I                   +P E  + + G K   L I   +  +  NY+CVASN +G  + NF +  +  P
Sbjct: 2787 DVIINNPLSLHCETNAAPPPTLTWYKDGRPLTSSDRVLILPGGRVLQ----IPRAKVEDAGRYTCVAVNEAGEDSLRYDVHVLLPPVIK-GANSDLPEEVTVLVNKSTQMECSSSGNPAPRNY-WQKDGQILLEDEHHKFQSDGRSLQILNAQITDTGRYVCVAENT----------AGSAKKYFNLNVHVP--PSVIGPNHEHLSVVVNHFISLNCEVS----GFPPPDLSWLKNEEPIKPNTNVLTVPGGRTLQIIRAKISDGGDYTCIAINQAGES--KKKVSLTVHVPPSI-KDHGSQSLSIVNVREGTSVSLECESNAVPPPVITWSK-NGRMIPDSTNVEILTGGQTLHIRRAEV---------------------SDTGQYVCRAINVAGRDDKNFHLNVYVPPTIEG--PETEVIVETISNPVTLTCDATGIPPPTITWLKNHKPI----------------ENSDPLEVHILSGGSK---LQIARPQRSNSGNYTCVASNMEGKAQKNFILFIQVPP 3244          

HSP 11 Score: 71.2478 bits (173), Expect = 9.738e-12
Identity = 100/441 (22.68%), Postives = 173/441 (39.23%), Query Frame = 3
Query:  345 ISHESRLTIHNISSDNSGYYVCRVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQW-----VIMQGLQIID--SSKFIAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLIHID--SNYRVPKNSNYSINCRVTSNPPPEFIKW-YKLSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIM-KGEKFIIKCSTDRKSPGEPFPTLKW------IDVETKDIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSI 1616
            I +   L I     D++G Y C    +  +      + +TV  PP+  S   + ++N   E     + C V+ GIP P I  +  KD       ++ +N      A+     +     LE+ G   C A N AG  T+  +L  +   P      P  ++    + + LSC+    P   + W     +I      I+  S   I K+  +  G  +C A+N +G  K    + V   P+   D  SN+  P   N  +NC V  +P P  I+W  K +++  S  +    +  L          G Y CV  N         +    E++M LT+      P I    + +++  G + I+ C    ++ GEP PT+ W      I  + +  +    +  +  +      +Y+C+A N +G    R   +V V+   S+
Sbjct: 4126 ILNSGALQIAFAQPDDAGQYTCMAANM--AGSSSVSSTLTVHVPPRIQSTEVHFTVN---ENSQAVLPC-VADGIPTPAI--HWEKDG------VLIANLLGKYTAQPYGELILENVVLEDSGTYTCVANNAAGEDTRIVTLA-VHTLPTFTEL-PGDLSLNKGEQLRLSCKAVGIPLPKLTWTFNNNIIPAHFDSINGHSELVIEKVSKEDSGTYVCTAENSVGFVKAIGFVYVKEPPVFKGDYPSNWIEPLGGNAILNCEVKGDPAPT-IQWSRKGADIEISHRIRQLGNGSLAIYGTVNEDAGDYTCVAAN---------EAGMVERSMSLTLQ---SSPIITLEPVETVVDAGGRVILDC----QAAGEPQPTITWSRQGQPISWDNRLSMLPNSSLYIAAARKEDTSEYECVARNLMGSVLVRVPVIVQVHGGFSL 4533          

HSP 12 Score: 66.6254 bits (161), Expect = 2.487e-10
Identity = 93/431 (21.58%), Postives = 171/431 (39.68%), Query Frame = 3
Query:  699 LENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQWV-IMQGLQIIDSSKF--IAKIRPDMDGPLICEAKNYL-------GSTKETVQLNVLRDPLIHIDSNYRVPKNSNY------SINCRVTSNPPPEFIKWYKLSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWI--DVETK-----DIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHG 1922
            L + GF  C A++ AG   +  +  ++   P I    PN V     +   L+C V +     + W    + +++ DS++   +A +  ++    I +A  Y        GS   +V L V   P + +     +PKN ++      SI C  T  P P+ +       +  S    ++ +  L          G YAC+  N              ++T  L     PK   ++    + +  G+  +++C    K+ G P P +KW   D+E +      I   +   ++  ++    G Y C+A NE G++  R T  + V   P  I +  +  V     ++  +++  C  +G P  + +WR   + +P  S+ F +     IS  R   +F               ++L +S  G Y CEA+N+ G
Sbjct:  491 LSDEGFYDCIAVSSAGT-GRAQTFFDVSEPPPIIQL-PNNVTVTPGERAVLACLVISAVDYNLTWQRSGRDIRLADSARIRTLANLSLELRSVKIGDAGEYRCVVSSEGGSAAASVFLTVQEKPKVTV-----MPKNQSFTGGSEISIMCSATGYPKPKIVWTMNEMFIMGSHRYRMTSEGTLFIKNAVPKDAGTYACLASNAA---------GTDKQTSTLRYIEAPK--LVVEQSELLVALGDTTVMEC----KTSGIPPPQVKWFKGDLELRPSTFLSIDPLVGLLKIQETQDLDAGDYTCVAINEAGRATGRLT--LDVGSPPVFIQEPSDVAV-----EIGSNVTLPCYVQGYPEPKIKWRR-LDNMPVFSRPFSVS---FISQLRTGALFI--------------SNLWASDKGTYICEAENQFG 874          

HSP 13 Score: 55.4546 bits (132), Expect = 6.277e-7
Identity = 84/365 (23.01%), Postives = 143/365 (39.18%), Query Frame = 3
Query:  702 ENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPF-VPNTVNAMINQTIDLSCEVYANPAATIQW-------VIMQGLQIIDSSKF--IAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDP-LIHID--SNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMTDSS--AVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKW-----IDVETKDIVSDMETFQVINSESRH---NGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWR 1727
            E+ G   C A N+AG ++K+  +L +   P I     P  V+ ++N  ++LSC     PA  I W       +  + +Q ++      ++  + +  G   C A +  G   +   + V   P +  +D   ++ V +N   ++ C+  + PPP  I W K      ++    ++S    L     D   T  Y CV  NI   TT         +   LTV   P      G + +  +  +   ++C    ++ G P P + W     +  E+    S +E    ++ ES H    G+Y C+A N  G    R    + V+  PSI     N  V ++        +  CE  G P     WR
Sbjct: 3498 EDTGKYTCVATNEAGEVSKHF-VLKVLEPPHINGSEGPGEVSVIVNNPLELSCIASGIPAPKISWMKDGRPFLQTEQVQTLEGGAILRVSSAQVEDTGRYTCLASSPAGDDDKEYLVRVHVPPNIAGMDEAQDFTVLRNRQVTLECKSDAVPPP-VIMWLKNREQLQATPRVRILSGGRYLQINNADLGDTANYTCVASNIAGKTT---------REFNLTVNVPPSIGG--GPQSLVTLLNKSIALEC----RAEGVPAPRITWRKDGVVLAESHARYSILEN-GFLHIESAHVTDTGRYLCMATNVAGT--DRRRIDLQVHVPPSIAMGPTNVTVTVN-----VQTTLACEATGIPKPSVTWR 3837          
BLAST of hemicentin-1 isoform X2 vs. Ensembl Mouse
Match: Robo2 (roundabout guidance receptor 2 [Source:MGI Symbol;Acc:MGI:1890110])

HSP 1 Score: 101.679 bits (252), Expect = 3.972e-21
Identity = 145/630 (23.02%), Postives = 238/630 (37.78%), Query Frame = 3
Query:  810 PNTVNAMINQTIDLSCEVYANPAATIQWVI-------------MQGLQIIDSSKFIAKI------RPDMDGPLICEAKNYLG---STKETVQLNVLRDPLIHIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMTDSSAVLIS-DDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFI-IKCSTDRKSPGEPFPTLKWIDVETKDI------VSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFT-KLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHG--IASLDYFLXXXXXXXXXXNIRHRKIAASLN-QNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFK---------------SQPDPPYDLRVTDFEWDSVTINWKPGFNGGLTQN-YEILVISENFGTQNVPFYSDKNENTLTITGLYPSTPYEIRIYAVNSIGRS 2549
            P+ V     +   L+C+    P  TI+W                  + +   S F  +I      +PD +G  +C A+NYLG   S   ++++ +LRD      ++  V       + C+     P   I W K     D     IS    KL      +S  GMY CV  N+     G+R    +E T+F       + P  L   I  ++  E+ +  +C       G+P PT++W   +  D+      + D  T ++  + S   G Y C+AEN +GK     +A +TV   P  + + +++ V        ++++F CE +G P     W+         SQ+    +     N R S   T  LTI          T+++ S  G Y C+A    G  +A     +           I    I  +L      +++C+A       I W K  E      +  + +I +                     L IK+L   D   Y+CVA++  G  + ++  V                   P PP   +VTD   +SVT++W+PG  G L  + Y I   S++         +       T+ GL P+T Y   + A+N  G S
Sbjct:   37 PSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPD-EGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNM----VGERDSDPAELTVF-------ERPTFLRRPINQVVLEEEAVEFRCQVQ----GDPQPTVRW-KKDDADLPRGRYDIKDDYTLRIKKAMSTDEGTYVCIAENRVGK--VEASATLTVRAPPQFVVRPRDQIVAQG-----RTVTFPCETKGNPQPAVFWQK------EGSQNLLFPNQPQQPNSRCSVSPTGDLTI----------TNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPINQTLAVDGTALLKCKATGEPLPVISWLK--EGFTFLGRDPRATIQDQ------------------GTLQIKNLRISDTGTYTCVATSSSG--ETSWSAVLDVTESGATISKNYDMNDLPGPPSKPQVTDVSKNSVTLSWQPGTPGVLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLS 604          

HSP 2 Score: 63.5438 bits (153), Expect = 2.023e-9
Identity = 99/454 (21.81%), Postives = 167/454 (36.78%), Query Frame = 3
Query:  360 RLTIHNISSDNSGYYVC--RVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVK---DNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMI--NQTIDLSCEVYANPAATIQWV-----IMQGLQII--DSSKFIAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLIHIDSNYR-VPKNSNYSINCRVTSNPPPEFIKWYKLSNMT---------DSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWI-------DVETKDIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQH 1628
            R+     S  + G YVC  R  L        SL V  ++       F  N +  V    E   ++C    G P P I     K   D+  +R+ I       S   +  A            G   C   N  G    + + L +  +P    F+   +N ++   + ++  C+V  +P  T++W      + +G   I  D +  I K     +G  +C A+N +G  + +  L V   P   +    + V +    +  C    NP P    W K  +            +S   +S    L    I RS  G Y C    +  S   K   A  E T  LT    P   Q   N+ +++      ++KC    K+ GEP P + W+         + +  + D  T Q+ N      G Y C+A +  G+  +  +A++ V E+ + I+++
Sbjct:   94 RIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRD-----DFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDA------------GMYTCVGTNMVGERDSDPAELTVFERPT---FLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKAMSTDEGTYVCIAENRVGKVEASATLTVRAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVF-WQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAK---AQLEVTDVLTDRPPPIILQGPINQTLAV--DGTALLKC----KATGEPLPVISWLKEGFTFLGRDPRATIQDQGTLQIKNLRISDTGTYTCVATSSSGE--TSWSAVLDVTESGATISKN 515          
BLAST of hemicentin-1 isoform X2 vs. UniProt/SwissProt
Match: sp|O89026|ROBO1_MOUSE (Roundabout homolog 1 OS=Mus musculus OX=10090 GN=Robo1 PE=1 SV=1)

HSP 1 Score: 107.071 bits (266), Expect = 7.274e-22
Identity = 148/617 (23.99%), Postives = 246/617 (39.87%), Query Frame = 3
Query:  849 LSCEVYANPAATIQWVIMQGLQIIDSSK---------------FIAKI------RPDMDGPLICEAKNYLG---STKETVQLNVLRDPLIHIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYK-LSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWI--DVE---TKDIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRY-GNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNV-----VMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQG-------IKKVNFEVVFKSQ--------PDPPYDLRVTDFEWDSVTINWKPGFNGGLT-QNYEILVISENFGTQNVPFYSDKNENTLTITGLYPSTPYEIRIYAVNSIG 2543
            L+C+    P  TI+W   +G + +++ K               F  +I      RPD +G  IC A+NYLG   S   ++++ +LRD      S+  V       + C+     P   I W K  S + D    +     KL      +S  G Y CV  N+     G+R+   +E    LTV  +P   +   N  +++    +F  KC    ++ G+P PT++W   D E   ++  + D  T ++    +   G Y C+AEN +GK  +  +A +TV E P  + + +++ V L      ++++F CE  G P     WR  G++ +      F  +  ++ S   +SQ    LTI          T+++ S  G Y C+  N  G      +L +      +     R+    +NQ V     +++ C A       I W K  + +++  Q  +    E+ V                  L I+  +  D   Y+C AS   G       I+   F V  +          P  P    VTD   ++VT++W+P  N G T  +Y I   S   G+       +    T  I GL P+  Y   + A N+ G
Sbjct:   48 LNCKAEGRPTPTIEW--YKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPD-EGVYICVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNM----VGERESEVAE----LTVLERPSFVKRPSNLAVTVDDSAEF--KC----EARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGK--AEASATLTVQEPPHFVVKPRDQVVALG-----RTVTFQCEATGNPQPAIFWRREGSQNL-----LFSYQPPQSSSRFSVSQT-GDLTI----------TNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQ--GPVNQTVAVDGTLILSCVATGSPAPTILWRK--DGVLVSTQDSRIKQLESGV------------------LQIRYAKLGDTGRYTCTASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSKNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTAAENVKTETFAIKGLKPNAIYLFLVRAANAYG 602          

HSP 2 Score: 76.6406 bits (187), Expect = 1.579e-12
Identity = 100/450 (22.22%), Postives = 165/450 (36.67%), Query Frame = 3
Query: 1041 LIHIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMTDS------SAVLISDDAKLNFILI-----DRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKW------IDVETKDIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVL-----PFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQG--IKKVNFEV--VFKSQPDP 2312
            ++   S+  V K    ++NC+    P P  I+WYK     ++      S  ++     L F+ I      R   G+Y CV +N             S +   L   ++     ++      +  GE  +++C   R   G P PT+ W      +D + + I        +  +     GKY C+  N +G+  S E A +TV E PS + +  N  V +       S  F CE RG P+   RWR  + ++P S   ++I D   +   +++                      +   G Y C A+N  G A     L ++ PP      R + +A       V  +C+A       I W +     +L     P  S ++S+ +                    +LTI +++  D   Y C   N  G  I K   EV  V   +P P
Sbjct:   31 IVEHPSDLIVSKGEPATLNCKAEGRPTPT-IEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYICVARNYLGEAVSHNA---SLEVAILRDDFRQNPSDVM------VAVGEPAVMECQPPR---GHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERES-EVAELTVLERPSFVKRPSNLAVTVD-----DSAEFKCEARGDPVPTVRWRKDDGELPKSR--YEIRDDHTLKIRKVT----------------------AGDMGSYTCVAENMVGKAEASATLTVQEPPHFVVKPRDQVVALG---RTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQ------------------TGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPP 416          

HSP 3 Score: 58.5362 bits (140), Expect = 5.339e-7
Identity = 97/434 (22.35%), Postives = 155/434 (35.71%), Query Frame = 3
Query:  360 RLTIHNISSDNSGYYVC--RVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVK---DNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFV--PNTVNAMINQTIDLSCEVYANPAATIQWVIMQG------LQIIDSSKF-IAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLIHIDSNYRVPK-NSNYSINCRVTSNPPPEFIKWYKLSNMT---------DSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKW----IDVETKDI-VSDMET--FQVINSESRHNGKYKCLAENEIGKS 1568
            R+     S  + G Y+C  R  L     H  SL V  ++       F  N S  +    E   ++C    G P P IS        D+  +R+ I               I+  R     + G  +C   N  G      + L +  +P    FV  P+ +   ++ + +  CE   +P  T++W    G       +I D     I K+     G   C A+N +G  + +  L V   P   +    +V       +  C  T NP P  I W +  +            SS   +S    L    + RS  G Y C T N+  S   K   A  E T  +     P   Q   N+ +++      I+ C       G P PT+ W    + V T+D  +  +E+   Q+  ++    G+Y C A    G++
Sbjct:   92 RIVHGRKSRPDEGVYICVARNYLGEAVSHNASLEVAILRD-----DFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKL---------MITYTR---KSDAGKYVCVGTNMVGERESEVAELTVLERPS---FVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPA-IFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITK---AYLEVTDVIADRPPPVIRQGPVNQTVAV--DGTLILSCVAT----GSPAPTILWRKDGVLVSTQDSRIKQLESGVLQIRYAKLGDTGRYTCTASTPSGEA 495          
BLAST of hemicentin-1 isoform X2 vs. UniProt/SwissProt
Match: sp|Q9NR99|MXRA5_HUMAN (Matrix-remodeling-associated protein 5 OS=Homo sapiens OX=9606 GN=MXRA5 PE=1 SV=3)

HSP 1 Score: 107.071 bits (266), Expect = 8.470e-22
Identity = 180/793 (22.70%), Postives = 310/793 (39.09%), Query Frame = 3
Query:  363 LTIHNISSDNSGYYVCRVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQWVIMQGLQIIDS-------------SKFIAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLIHIDSNYRV----PKNSN------YSINCRVTSNPPPEFIKWYKLSNMTDSSAVLISDDAKL----NFILIDRSST----GMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETKDIVSDMET---------FQVINSES--------RHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARW-RYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXN-----IRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEV-VFKSQPDP----PYDLRVTDFEWDSVTINWKPGFNGGLTQNYEILVISENF-----GTQNVPFYSDKNENTLTITGLYPSTPYEIRIYAVNSIGRS 2549
            L I  +   + G Y+C  + L   + ++ L  VTV+ P + ++ H+ + + V+   + IA++C ++ G P P+IS +I  D  +   + +S      T+ E   +S+    +  +RG   C A N AG  +    L      P I       ++     +I + C   A P  +++WV+  G QI  S             + +I  + P   G   C A N +GS + TVQLNV R       +N R+    P+ ++        ++C  + +P P  +       M D+   L S D+++    N  L+ +S T    G Y CV +N         K  +    + + V  KP + +        +  G    + C       G P P + W   +   + S M++         + V N+ +        R  G Y C AEN++GK   R    V V  AP+ I       V + Y D+   ++  CE +G+P+ +  W    N+ IP SS+ +              QI+   T+ +       Q + RS  +G Y C   N  G      ++ +   P             R+IAA  ++   +I C+AE I T  + W    E +VLP        +   +T +               L I+ L   D     C+A N+ G  ++  ++ V +    P    P   ++T     ++++N     +   T    ++ +  N      G Q   FY  K +  L I+GL        R  A N+ G +
Sbjct: 1913 LVIRKVQVQDRGQYMCTASNLHGLDRMVVLLSVTVQQP-QILASHY-QDVTVYL-GDTIAMEC-LAKGTPAPQIS-WIFPDRRV--WQTVSPVEGRITLHENRTLSIKEASF-SDRGVYKCVASNAAGADSLAIRLHVAALPPVIHQEKLENISLPPGLSIHIHCTAKAAPLPSVRWVLGDGTQIRPSQFLHGNLFVFPNGTLYIRNLAPKDSGRYECVAANLVGSARRTVQLNVQR-----AAANARITGTSPRRTDVRYGGTLKLDCSASGDPWPRILWRLPSKRMIDA---LFSFDSRIKVFANGTLVVKSVTDKDAGDYLCVARN---------KVGDDYVVLKVDVVMKPAKIEHKEENDHKVFYGGDLKVDCVAT----GLPNPEISWSLPDGSLVNSFMQSDDSGGRTKRYVVFNNGTLYFNEVGMREEGDYTCFAENQVGKDEMR--VRVKVVTAPATIRNKTYLAVQVPYGDV---VTVACEAKGEPMPKVTWLSPTNKVIPTSSEKY--------------QIYQDGTLLI-------QKAQRSD-SGNYTCLVRNSAGEDRKTVWIHVNVQPPKINGNPNPITTVREIAAGGSRK--LIDCKAEGIPTPRVLW-AFPEGVVLP-----APYYGNRITVH-----------GNGSLDIRSLRKSDSVQLVCMARNEGGEARLILQLTVLEPMEKPIFHDPISEKITAMAGHTISLN----CSAAGTPTPSLVWVLPNGTDLQSGQQLQRFY-HKADGMLHISGLSSVDAGAYRCVARNAAGHT 2625          

HSP 2 Score: 69.707 bits (169), Expect = 2.301e-10
Identity = 158/711 (22.22%), Postives = 261/711 (36.71%), Query Frame = 3
Query:  330 ESQITISHESR-LTIHNISSDNSGYYVCRVTLLTTSEHI-ISLAVVTVKTPPKSISFHFNKSINVFQEQEF-IAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSN---FPNSTIAEVSAISLWRPHYLEN-----RGFVMCTAINDAGVLTKNTSLLNIKYKP---KIKPFVPNTVNAMINQTIDLSCEVYANPAATIQWVIMQG------------LQIIDSSKFIAKIRPDMD-GPLICEAKNYLGSTKETVQLNVLRDP--LIHIDSN-YRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMTDSSAV---LISDDAK-------------LNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKI---ISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETKDIVSDMETFQVIN--------SESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKN-----RKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSS-QDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIA---ASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQG 2264
            E +IT+ HE+R L+I   S  + G Y C  +    ++ + I L V  +  PP     H  K  N+       I I C+     P P +   +     I+  + +  N   FPN T+            Y+ N      G   C A N  G   + T  LN++      +I    P   +     T+ L C    +P   I W +               +++  +   + K   D D G  +C A+N +G     ++++V+  P  + H + N ++V    +  ++C  T  P PE I W    ++ D S V   + SDD+              L F  +     G Y C  +N      GK    +  +     VT     P  + NK    + +  G+   + C    ++ GEP P + W+    K I +  E +Q+          ++   +G Y CL  N  G+   R+T  + VN  P  I  + N     R++    S         C+  G P     W +    + P+     +I  H N S            +D+         SLR S + +  C A N  G A L   L +     ++  I H  I+    ++  + + + C A    T  + W       VLP  +   S    ++ ++  + D    G+    L I  L + D   Y CVA N  G
Sbjct: 1999 EGRITL-HENRTLSIKEASFSDRGVYKCVASNAAGADSLAIRLHVAAL--PP---VIHQEKLENISLPPGLSIHIHCTAKAA-PLPSVRWVLGDGTQIRPSQFLHGNLFVFPNGTL------------YIRNLAPKDSGRYECVAANLVGS-ARRTVQLNVQRAAANARITGTSPRRTDVRYGGTLKLDCSASGDPWPRILWRLPSKRMIDALFSFDSRIKVFANGTLVVKSVTDKDAGDYLCVARNKVGDDYVVLKVDVVMKPAKIEHKEENDHKVFYGGDLKVDCVATGLPNPE-ISW----SLPDGSLVNSFMQSDDSGGRTKRYVVFNNGTLYFNEVGMREEGDYTCFAEN----QVGK----DEMRVRVKVVT----APATIRNKTYLAVQVPYGDVVTVAC----EAKGEPMPKVTWLSPTNKVIPTSSEKYQIYQDGTLLIQKAQRSDSGNYTCLVRNSAGE--DRKTVWIHVNVQPPKINGNPNPITTVREIAAGGS----RKLIDCKAEGIPTPRVLWAFPEGVVLPAPYYGNRITVHGNGS------------LDIR--------SLRKSDSVQLVCMARNEGGEARL--ILQLTVLEPMEKPIFHDPISEKITAMAGHTISLNCSAAGTPTPSLVW-------VLPNGTDLQS--GQQLQRFYHKAD----GM----LHISGLSSVDAGAYRCVARNAAG 2623          
BLAST of hemicentin-1 isoform X2 vs. UniProt/SwissProt
Match: sp|Q9Y6N7|ROBO1_HUMAN (Roundabout homolog 1 OS=Homo sapiens OX=9606 GN=ROBO1 PE=1 SV=1)

HSP 1 Score: 105.145 bits (261), Expect = 2.528e-21
Identity = 157/666 (23.57%), Postives = 264/666 (39.64%), Query Frame = 3
Query:  705 NRGFVMCTAINDAGVLTKNTSLLNIK-YKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQWVIMQGLQIIDSSK---------------FIAKI------RPDMDGPLICEAKNYLG---STKETVQLNVLRDPLIHIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYK-LSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWI--DVE---TKDIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRY-GNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVV-----MIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQG-------IKKVNFEVVFKSQ--------PDPPYDLRVTDFEWDSVTINWKPGFNGGLT-QNYEILVISENFGTQNVPFYSDKNENTLTITGLYPSTPYEIRIYAVNSIG 2543
            + G  + T+ ND   L    S L  + + P+I    P+ +     +   L+C+    P  TI+W   +G + +++ K               F  +I      RPD +G  +C A+NYLG   S   ++++ +LRD      S+  V       + C+     P   I W K  S + D    +     KL      +S  G Y CV  N+     G+R+   +E    LTV  +P   +   N  +++    +F  KC    ++ G+P PT++W   D E   ++  + D  T ++    +   G Y C+AEN +GK  +  +A +TV E P  + + +++ V L      ++++F CE  G P     WR  G++ +      F  +  ++ S   +SQ    LTI          T+++ S  G Y C+  N  G      +L +      +     R+    +NQ V      ++ C A       I W K  + +++  Q  +    E  V                  L I+  +  D   Y+C+AS   G       I+   F V  +          P  P    VTD   ++VT++W+P  N G T  +Y I   S   G+       +    T  I GL P+  Y   + A N+ G
Sbjct:   39 DHGTPIPTSDNDDNSLGYTGSRLRQEDFPPRIVEH-PSDLIVSKGEPATLNCKAEGRPTPTIEW--YKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPD-EGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNM----VGERESEVAE----LTVLERPSFVKRPSNLAVTVDDSAEF--KC----EARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGK--AEASATLTVQEPPHFVVKPRDQVVALG-----RTVTFQCEATGNPQPAIFWRREGSQNL-----LFSYQPPQSSSRFSVSQT-GDLTI----------TNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQ--GPVNQTVAVDGTFVLSCVATGSPVPTILWRK--DGVLVSTQDSRIKQLENGV------------------LQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETSAIKGLKPNAIYLFLVRAANAYG 641          
BLAST of hemicentin-1 isoform X2 vs. UniProt/SwissProt
Match: sp|D3YXG0|HMCN1_MOUSE (Hemicentin-1 OS=Mus musculus OX=10090 GN=Hmcn1 PE=1 SV=1)

HSP 1 Score: 103.219 bits (256), Expect = 1.302e-20
Identity = 168/689 (24.38%), Postives = 265/689 (38.46%), Query Frame = 3
Query:  363 LTIHNISSDNSGYYVCRVTLLTTSEHIISLAVVTVKTPPKSIS-FHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSA---ISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNT---VNAMINQTIDLSCEVYANPAATIQWVIMQGLQIIDSSKF----------IAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLIHI-DSNYRVPKNSN-YSINCRVTSNPPPEFIKWYK----LSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKI---ISIMKGEKFIIKCSTDRKSPGEPFPTLKWI---------DVETKDIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVN--EAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQPDPPY 2318
            L I N    ++G YVC      T+        + V  PP  I   H + S+ V     FI++ C VS G PPP +S ++  +  IK         PN+ +  V     + + R   + + G   C AIN AG   K  S L +   P IK     +   VN     ++ L CE  A P   I W    G  I DS+            I +      G  +C A N  G   +   LNV   P I   ++   V   SN  ++ C  T  PPP  I W K    + N       ++S  +KL      RS++G Y CV  N++    GK   A     +F+ V      P + G ++   +S++ GE   + C+ D    G P P L+W+         + E   + +D  T  +  + +   GKY C+A N  G+    E  +  +N    P I    +  +  ++  D   SI+  C+  G P  +  W      +P SS        R +S  ++ +I      D+                  Y C A NR G+ S  Y L +  PP +   +   +I      +  M  C  +      + W +  + +            +A +T       + T G+    L +   E +D   Y+CVA+N+ G  +V+   V K   +PP+
Sbjct: 2927 LQILNAQITDTGRYVCVAE--NTAGSAKKYFNLNVHVPPSVIGPNHEHLSVVV---NHFISLNCEVS-GFPPPDLS-WLKNEEPIK---------PNTNVLTVPGGRTLQIIRAK-ISDGGDYTCIAINQAGESKKKVS-LTVHVPPSIKDHGSQSLSIVNVREGTSVSLECESNAVPPPVITWS-KNGRMIPDSTNVEILTGGQTLHIRRAEVSDTGQYVCRAINVAGRDDKNFHLNVYVPPTIEGPETEVIVETISNPVTLTCDATGIPPPT-ITWLKNHKPIENSDPLEVHILSGGSKLQIARPQRSNSGNYTCVASNME----GK---AQKNFILFIQV-----PPSVAGAEVPSEVSVLLGENVELVCNAD----GIPTPHLQWLRDGKPIVNGETERVRVTTDGSTLNIYRALTSDMGKYTCVATNPAGE----EDRIFNLNVYVPPKIRGNKEEAEKLMALVD--TSINIECKATGTPPPQINWLKNGLPLPISSH------IRLLSAGQVVRIVRAQVSDI----------------AVYTCVASNRAGVDSKHYSLQVFVPPNMDNAMGTEEITIVKGSSTSMT-CFTDGTPAPSMSWLRDGQPLAP----------DAHLT-------VSTQGMV---LQLIKAETEDTGKYTCVATNEAG--EVSKHFVLKVL-EPPH 3527          

HSP 2 Score: 103.219 bits (256), Expect = 1.554e-20
Identity = 154/697 (22.09%), Postives = 258/697 (37.02%), Query Frame = 3
Query:  315 VVPDSESQITISHESRLTIHNISSDNSGYYVCRVTLLTTSE----HIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNST--IAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPF-VPNTVNAMINQTIDLSCEVYANPAATIQWVIMQGLQIIDSSKFIAKIRPDMD------------GPLICEAKNYLGSTKETVQLNVLRDPLIHID----SNYRVPKNSNYSINCRVTSNPPPEFIKWYK--LSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETKDIVSDME--------TFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQP 2306
            ++PDS +   ++    L I      ++G YVCR   +   +    H+      T++ P           + V      + + C  +G IPPP I+        +K  K I ++ P     ++  S + + RP    N G   C A N  G   KN  +L I+  P +    VP+ V+ ++ + ++L C     P   +QW +  G  I++      ++  D              G   C A N  G       LNV   P I  +           +++ +I C+ T  PPP+ I W K  L     S   L+S    +  +    S   +Y CV  N         +     K   L V   P     +G + I+I+KG    + C TD    G P P++ W+  + + +  D            Q+I +E+   GKY C+A NE G+  S+   L  V E P I       +V +  ++    +  +C   G P  +  W               ++D R         + T+    L      + +S +    GRY C A +  G    +Y + +  PP I      +      N+ V +  C+++++    I W K  E +                 +  P   + + G     L I + +  D +NY+CVASN  G     F +     P
Sbjct: 3098 MIPDSTNVEILTGGQTLHIRRAEVSDTGQYVCRAINVAGRDDKNFHLNVYVPPTIEGP--------ETEVIVETISNPVTLTCDATG-IPPPTITW-------LKNHKPIENSDPLEVHILSGGSKLQIARPQR-SNSGNYTCVASNMEGKAQKNF-ILFIQVPPSVAGAEVPSEVSVLLGENVELVCNADGIPTPHLQW-LRDGKPIVNGETERVRVTTDGSTLNIYRALTSDMGKYTCVATNPAGEEDRIFNLNVYVPPKIRGNKEEAEKLMALVDTSINIECKATGTPPPQ-INWLKNGLPLPISSHIRLLSAGQVVRIVRAQVSDIAVYTCVASN---------RAGVDSKHYSLQVFVPPNMDNAMGTEEITIVKGSSTSMTCFTD----GTPAPSMSWLR-DGQPLAPDAHLTVSTQGMVLQLIKAETEDTGKYTCVATNEAGE-VSKHFVL-KVLEPPHINGSEGPGEVSVIVNN---PLELSCIASGIPAPKISW---------------MKDGRPF-------LQTEQVQTLEGGAILRVSSAQVEDTGRYTCLASSPAGDDDKEYLVRVHVPPNIAGMDEAQDFTVLRNRQVTL-ECKSDAVPPPVIMWLKNREQL-----------------QATPRVRILSGG---RYLQINNADLGDTANYTCVASNIAGKTTREFNLTVNVPP 3712          

HSP 3 Score: 99.7525 bits (247), Expect = 1.688e-19
Identity = 151/698 (21.63%), Postives = 266/698 (38.11%), Query Frame = 3
Query:  315 VVPDSESQITI-SHESRLTIHNISSDNSGYYVC---RVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKI--KPFVPNTVNAMINQTIDLSCEVYANPAATIQWV-------IMQGLQIIDSSKFI--AKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLIHIDS---NYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNM--TDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETK-------DIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLP----QSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDY---------FLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEV 2288
            V+ DS  ++ I S   RL I      ++G Y C    V  +   E+ + + +     P  + S      I V + +  I+++C V G IP P ++ ++     + + K +        I +   I   +  ++ + G  +C A+N AG+  K   L ++   P I     VP  V+ +   ++ L+CE    P  +I W+       +   ++I+   + +   + RP+  G   C  +N  G  ++   L+VL  P I  ++   + ++ +  + ++ C V  NP P+ I W+K   +   D +  ++S    L       S TG Y C+  NI        K  +    +F++ T    +       +I I+     ++ C      P     T+ W    T         I+    T Q++N++  + G+Y C+A NE G+        V +   P II +       LS  ++      S++  CE    P    RW           Q  K +DH              +TI     T  Q    + S  GRY C A N  G   LD+         F  +     +    R  +    +  N + + C+  +     + WYK       P  S               +R L  PG +V  L I   + +D   Y+CVA N+ G   + ++V
Sbjct: 2236 VLADSAGRVHILSGGRRLQISIAEKADAGLYTCVASNVAGVAKKEYNLQVYI----RPSITNSGGHRPEITVIRGKS-ISLECEVQG-IPQPTVT-WMKDGRPLTKGKGVE-------ILDEGRILQLKNVHVSDTGRYVCVAVNVAGMTDKRYDL-SVHAPPSIIGNHGVPENVSVVEKSSVSLTCEASGIPLPSITWLKDGWPVNLGSSVKILSGGRMLRLMQTRPEDAGQYTCIVRNAAGEDRKMFGLSVLVPPHIVGENTLEDVKIKEKQSVTLTCEVRGNPVPQ-ITWHKDGQLLQEDEAHHMMSGGRFLQITNAQVSHTGRYTCLASNI-----AGDKSKSFRLNVFVSPTIAGVDSDGSPEDVIVILNSPTSLV-CEAYSYPPA----TITWFKDGTPLESNRNIRILPGGRTLQILNAQEDNAGRYSCVATNEAGEKIKHYEVKVYI---PPIIKKGDLLGPGLSPKEVKIRVNSSLTLECEAYAIPSASLRWYK-------DGQPLKSDDH--------------VTIAASGHTL-QIKEAQISDTGRYTCVASNLAGEDELDFDVNIQVPPSFQKLWEIGNMLDTGRSGEAKDVIINNPLSLHCETNAAPPPTLTWYKDGR----PLTSS--------------DRVLILPGGRV--LQIPRAKVEDAGRYTCVAVNEAGEDSLRYDV 2862          

HSP 4 Score: 93.5893 bits (231), Expect = 1.305e-17
Identity = 152/679 (22.39%), Postives = 265/679 (39.03%), Query Frame = 3
Query:  363 LTIHNISSDNSGYYVCRVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRL------KIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQWVIMQGLQIIDSSKFIA-----------KIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLIHI-DSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSN-MTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKI-ISIMKGEKFIIKCSTDRKSPGEPFPTLKW-------IDVETKDIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRET----ALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQP 2306
            L I + S D++  Y C VT  + +        +TV+ PP +I+   ++ ++    ++  A+    + G+P P I       N ++ L      +I+SS           AI +     L + G   C A N AG   ++ +L  ++  P I+P  P+ ++ ++N  I L CE    P   I W   +G+ +I S K +A            +R D  G  +C A+N  G+    V+LNV   P+I      Y V  +   S+ C    +PPP+ I W+K  + +T+S    I +   L          G Y C+  N+           +S  +  LTV   P+   I   ++  ++ +  + ++ C  D    G P P + W        ++  K          + N     +G Y C+A N  G+     T     L T  E P  ++ +K  ++ LS           C+  G PL +  W + N  IP  +    I  H  +  E++S+                        +G Y C A+N  G      F+ +K PP+ K +     I   L  N ++  C+ +      I+W +    I +  + ++                     L    L I    N+D  +Y+CVA+N+ G+ + +  +  +S P
Sbjct: 3860 LVIISPSVDDTASYECTVT--SDAGEDKRAVDLTVQVPP-TIA---DEPMDFLVTRQAPAVMTCSASGVPVPSIHW---TKNGLRLLPRGDGYRILSSG----------AIEIPTTQ-LNHAGRYTCVARNAAGSAHRHVTL-RVQEPPVIQP-QPSELDVILNNPILLPCEATGIPTPFITWQ-KEGINVITSGKSLAILPSGSLQISRAVRGDA-GTYMCVAQNPAGTALGKVKLNVQVPPVISSHQKEYVVTMDKPVSLLCETEGSPPPD-ITWHKDGHALTESIRQRILNSGALQIAFAQPDDAGQYTCMAANMA---------GSSSVSSTLTVHVPPR---IQSTEVHFTVNENSQAVLPCVAD----GIPTPAIHWEKDGVLIANLLGKYTAQPYGELILENVVLEDSGTYTCVANNAAGEDTRIVTLAVHTLPTFTELPGDLSLNKGEQLRLS-----------CKAVGIPLPKLTWTFNNNIIP--AHFDSINGHSELVIEKVSK----------------------EDSGTYVCTAENSVGFVKAIGFVYVKEPPVFKGDYPSNWIEP-LGGNAIL-NCEVKGDPAPTIQWSRKGADIEISHRIRQ---------------------LGNGSLAIYGTVNEDAGDYTCVAANEAGMVERSMSLTLQSSP 4439          

HSP 5 Score: 90.5077 bits (223), Expect = 9.450e-17
Identity = 130/553 (23.51%), Postives = 217/553 (39.24%), Query Frame = 3
Query:  699 LENRGFVMCTAINDAGVLTKNTSL---LNIKYKPKIKPF---VPNTVNAMINQTIDLSCEVYANPAATIQWVIMQGLQII---DSSKFIAKIRPDM--------DGPLICEAKNYLGSTKETVQLNVLRDPLIHIDSNYR---VPKNSNYSINCRVTSNPPPEFIKWYKLSN--MTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKP--KEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWI-------DVETKDIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQG 2264
            +E+ G   C A+N+AG      SL   +++   P IK     +P  V  ++N++  + C    NPA    W   +  QI+   +  KF +  R            G  +C A+N  GS K+   LNV   P + I  N+    V  N   S+NC V+  PPP+ + W K       +++ + +     L  I    S  G Y C+  N         +   S+K + LTV   P  K+       I+++ +G    ++C ++   P    P + W        D    +I++  +T  +  +E    G+Y C A N  G+        V V   P  I   +   +  + S+    ++ TC+  G P     W               +++H+ I N    ++       L   +  Q    + S +G Y C A N  G A  ++ L I+ PP +       +++  L +NV ++ C A+ I T H++W +  + IV                  E ER   T     + L I      D   Y+CVA+N  G
Sbjct: 2839 VEDAGRYTCVAVNEAG----EDSLRYDVHVLLPPVIKGANSDLPEEVTVLVNKSTQMECSSSGNPAPRNYW--QKDGQILLEDEHHKFQSDGRSLQILNAQITDTGRYVCVAENTAGSAKKYFNLNVHVPPSV-IGPNHEHLSVVVNHFISLNCEVSGFPPPD-LSWLKNEEPIKPNTNVLTVPGGRTLQIIRAKISDGGDYTCIAIN---------QAGESKKKVSLTVHVPPSIKDHGSQSLSIVNVREGTSVSLECESNAVPP----PVITWSKNGRMIPDSTNVEILTGGQTLHIRRAEVSDTGQYVCRAINVAGRDDKNFHLNVYV---PPTIEGPETEVIVETISN---PVTLTCDATGIPPPTITW---------------LKNHKPIENSDPLEVHI-----LSGGSKLQIARPQRSNSGNYTCVASNMEGKAQKNFILFIQVPPSVAGAEVPSEVSVLLGENVELV-CNADGIPTPHLQWLRDGKPIV----------------NGETERVRVT--TDGSTLNIYRALTSDMGKYTCVATNPAG 3325          

HSP 6 Score: 90.1225 bits (222), Expect = 1.313e-16
Identity = 162/689 (23.51%), Postives = 254/689 (36.87%), Query Frame = 3
Query:  348 SHESRLTIHNISSDNSGYYVCRVTLLTTSEHIISLAVVTVKTPP--KSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKI--KPFVPNTVNAMINQTIDLSCEVYANPAATIQW-------VIMQGLQIIDSSKFIA--KIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLI---HIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSN---MTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKI---ISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETKDIVSDMETFQVINSESRH--------NGKYKCLAENEIG---KSFSRETALVTVNEAPSI---ITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQP 2306
            S +  L+I      ++G Y C  T +  S+   +   + V+ PP  + +   FN         + I   C   G  P P I         +   + ++   P  +I E  A+ +       N G  +C A+N+AG  T+    L +   P I  K  V N V+ + +Q   L CEV   P+  I W            +QI+++ K +   K+  +  G   C+A N  G++++   +NVL  P I      S   V  N N ++ C  T  P P  I W+K      + D +  L      L+     RS  G Y C   N      GK+      K + LTV Y P  P I G  I   IS +      ++C T     G P P + W   + + + S  +   +   +  H        +  Y C A N  G   KSF      V +   P+I   +T   N++V +      QS+   C+  G P     W    + +P  + D     H     +++ +I + L +D                 G+Y C A +  G   + Y + +  PP ++           L  N++ + CQ        I W K  + I                     ERD F   L   +L I   +  D   Y CVA+N  G  +  FEV     P
Sbjct: 1220 SPDGTLSIEQAVISDAGVYTCAATNIAGSDE--AEVTLHVQEPPSVEDLQPPFNTPFQERLANQRIEFPCPAKG-TPKPTIKW-------LHNGREVTGQEPGVSILEDGALLVIASVTPHNNGEYICVAVNEAGT-TERKYNLKVHVPPVIRDKEHVTN-VSVLTSQLASLYCEVEGTPSPVITWYKDDIQVTESSTVQIVNNGKILKLFKVSAEDAGRYSCKAINIAGTSQKDFSVNVLVPPSILGASSPSEVSVVLNHNVTLQCPGTGVPFPA-IHWFKDGKPLFLGDPNIELSDRGQSLHLRNARRSDKGRYQCTVSN----AAGKQA-----KDIKLTV-YVP--PSIKGGNITTEISALLNSIVKLECETR----GLPVPAITWYK-DGQVVTSSSQALYIDKGQLLHIQRAQVSDSATYTCHAANVAGTAEKSFH-----VDIYVPPTIEGDLTAPSNKQVIIG-----QSLILECKAAGNPPPILTWL--KDGVPVKASD---NIHIEAGGKKL-EILSALEVD----------------RGQYICVATSVAGEREIKYEVDVLVPPAVEGG-EETSYFIVLANNLLELDCQVSGSPPPTIMWLKGGQLI--------------------DERDGFKILLNGRKLVIAQAQVSDTGLYQCVATNIAGDHRKEFEVTVHVPP 1825          

HSP 7 Score: 77.7962 bits (190), Expect = 8.212e-13
Identity = 175/741 (23.62%), Postives = 279/741 (37.65%), Query Frame = 3
Query:  159 PNNATVNSKENLTLEC-VVDQQNIVYSWEYKPFHIKSNIMSKVLGFCYFEKPCVVPDSESQITISHESRLTIHNISSDNSGYYVCRVTLLTTSEHIISLAVV--TVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKI-----SMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQWVIMQGLQIIDSSKFIA--------KIRPDMD---GPLICEAKNYLGSTKETVQLNVLRDPL-IHIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMTDSS---AVLISDDAKLNFILIDR---SSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILG--NKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETKDI------VSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRH----RKIAAS-LNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQG 2264
            PNN TV   E   L C V+   +   +W+     I+                  + DS    T+++ S L + ++   ++G Y C V    +SE   + A V  TV+  PK      N+S   F     I+I CS + G P PKI      M+I+  +   R ++ S      T+   +A+        ++ G   C A N AG   K TS L     PK+       + A+ + T+ + C+    P   ++W   +G   +  S F++        KI+   D   G   C A N  G     + L+V   P+ I   S+  V   SN ++ C V   P P+ IKW +L NM   S   +V      +   + I     S  G Y C  +N      GK +   S+ T+ +T    P    ++G    + S+++G+   + C+      G P P  +W+      +      V    +  +     +  GKY C+A N  G   + +T  V V+  PSI  QH  +++  +   +P  ++  C   G P     W    E I  SS  F      +                LY        S  S  +G Y C A N  G A     L +   P +  + R     + +  S L     ++ C+A+S+    I W K +  ++ PF                  R  F P      + I +    D   Y CVA+N  G
Sbjct:  526 PNNVTVTPGERAVLACLVISAVDYNLTWQRSGRDIR------------------LADSARIRTLANLS-LELRSVKIGDAGEYRCVV----SSEGGSAAASVFLTVQEKPKVTVMPKNQS---FTGGSEISIMCSAT-GYPKPKIVWTMNEMFIMGSH---RYRMTS----EGTLFIKNAVP-------KDAGTYACLASNAAGT-DKQTSTLRYIEAPKLVVEQSELLVALGDTTV-MECKTSGIPPPQVKW--FKGDLELRPSTFLSIDPLVGLLKIQETQDLDAGDYTCVAINEAGRATGRLTLDVGSPPVFIQEPSDVAVEIGSNVTLPCYVQGYPEPK-IKWRRLDNMPVFSRPFSVSFISQLRTGALFISNLWASDKGTYICEAEN----QFGKIQ---SQTTVTVTGLVAP----LIGISPSMASVIEGQPLTLPCTL---LAGNPIPERRWMKNSAMLVQNPYITVRSDGSLHIERVRLQDGGKYTCVASNVAGT--NNKTTSVAVHVLPSI--QH-GQQILSTIEGVP--VTLPCRASGIPKPSITWSKKGELISTSSAKFSAGADGS----------------LYV------VSPGSEESGEYICTATNAAGYAKRKVQLTVYVRPRVFGDQRGLSQDKPVEISVLAGEEAILPCEAKSLPPPIITWAK-DSQLISPFSP----------------RHTFLPS---GSMKITETRVSDSGMYLCVATNIAG 1157          

HSP 8 Score: 77.7962 bits (190), Expect = 8.351e-13
Identity = 145/669 (21.67%), Postives = 246/669 (36.77%), Query Frame = 3
Query:  390 NSGYYVCRVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKR---LKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQWV--------IMQGLQIIDSSKF--IAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLIHIDSNYR---VPKNSNYSINCRVTSNPPPEFIKWYKLSN--MTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNK-IISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETK--------DIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNE---RISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQP 2306
            + G Y+C  T +     I     V V   P ++      S  +      + + C VSG  PPP I M++     I      KI+  N     IA+           + + G   C A N AG   K   +  +   P IK         +  + + L C     P  +I W+            L+I  S +   IAK   +  G   C A N  G   +  QL+V   P +      R   V  N+   + C+ T  P P  I WYK S+     +SA  +     L       S  G+Y CV  N           A + +  +    + P  P I G+  ++ ++      ++C    ++ G P P+L W+   +          I+S      + +++    G+Y C+A N  G+   R+  L  V   P+I+ + +N  V +      Q++   C+              ++ +PP +  + ++D R +       IS+  + L I+            ++   GRY CEA N  G    +Y + +  PP I  +    ++ A +  N++ + C++  I    + W K    +VL   + +  I                       L I   E  D   Y+CVASN  G+ K  + +    +P
Sbjct: 1705 DRGQYICVATSVAGEREIKYEVDVLV---PPAVEGGEETSYFIVLANNLLELDCQVSGS-PPPTI-MWLKGGQLIDERDGFKILL-NGRKLVIAQAQ---------VSDTGLYQCVATNIAGDHRKEFEV-TVHVPPTIKSSDLPEKTVVRYKPVTLQCIANGIPNPSITWLKDDQPVNTAHGNLKIQSSGRVLQIAKALLEDAGRYTCVATNAAGEAHQHTQLHVHEPPSLDDAGKMRNETVVVNNPIQLECKATGKPLP-VITWYKDSHPLSGSASAAFLKRGQVLEIGSAQISDAGIYKCVAIN----------SAGATELFYSLQVHVP--PSISGSSSMVEVVVNNLARLEC----EARGIPAPSLTWLKDGSPVSSFSNGIQILSGGRILALTSAQMSDAGRYTCVAVNAAGEK-QRDIDL-RVYAPPNIMGEEQNVSVLIG-----QAVELFCQ--------------SDAVPPPTLMW-LKDGRPLLKRPGLSISENGSVLKIE----------DAQAGDTGRYTCEATNVAGKTEKNYNVNVWVPPSIYGSDELVQLTA-IEGNLITLLCESSGIPPPDLTWKKKGS-LVLADSAGRVHILSGG-----------------RRLQISIAEKADAGLYTCVASNVAGVAKKEYNLQVYIRP 2289          

HSP 9 Score: 76.6406 bits (187), Expect = 1.708e-12
Identity = 165/743 (22.21%), Postives = 254/743 (34.19%), Query Frame = 3
Query:  147 FLITPNNATVNSKENLTLECVVDQQNIVYSWEYKPFHIKSNIMSKVLGFCYFEKPCVVPDSESQITISHESRLTIHNISSDNSGYYVCRV-TLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQWVIMQGLQIIDSSKFIAK-------IRPDMD--GPLICEAKNYLGSTKETVQLNVLRDPLIHIDS---------NYRVPKNSNYSINCRVTSNPPPEFIKWYK----LSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDV------ETKDIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEV 2288
            F+  P++  V    N+TL C        Y   Y    IK   +  +  F         P S S I+      L I N+ + + G Y+C         +   ++ V  +  P   IS      I    E + + + C++  G P P+          +K   ++  N P  T+    ++ + R   L++ G   C A N AG   K TS+  +   P I+      ++ +    + L C     P  +I W     L    S+KF A        + P  +  G  IC A N  G  K  VQL V   P +  D             V       + C   S PPP  I W K    +S  +     L S   K+    +  S +GMY CV  NI           N  +++ L+V   PK     GN+ I +  G++  I C+      G P P + W           +   S   T  +  +     G Y C A N  G   +  T  + V E PS+                 Q I F C  +G P    +W +   ++        I +   +           L I           S+    NG Y C A N  G     Y L   + P +  +  H    + L   +  + C+ E   +  I WYK  + I +   S    +   ++ K      LF             +  +D   YSC A N  G  + +F V
Sbjct:  795 FIQEPSDVAVEIGSNVTLPC--------YVQGYPEPKIKWRRLDNMPVFSR-------PFSVSFISQLRTGALFISNLWASDKGTYICEAENQFGKIQSQTTVTVTGLVAPLIGISPSMASVI----EGQPLTLPCTLLAGNPIPE-------RRWMKNSAMLVQN-PYITVRSDGSLHIERVR-LQDGGKYTCVASNVAGTNNKTTSVA-VHVLPSIQ-HGQQILSTIEGVPVTLPCRASGIPKPSITWSKKGELISTSSAKFSAGADGSLYVVSPGSEESGEYICTATNAAGYAKRKVQLTVYVRPRVFGDQRGLSQDKPVEISVLAGEEAILPCEAKSLPPP-IITWAKDSQLISPFSPRHTFLPSGSMKITETRV--SDSGMYLCVATNIA---------GNVTQSVKLSVHVPPKIQH--GNRHIKVQVGQRVDILCNAH----GSPPPVITWFKSGRPFLDGAQHPGSPDGTLSIEQAVISDAGVYTCAATNIAGSDEAEVT--LHVQEPPSVEDLQPPFNTPFQERLANQRIEFPCPAKGTPKPTIKWLHNGREVTGQEPGVSILEDGAL-----------LVI----------ASVTPHNNGEYICVAVNEAGTTERKYNL-KVHVPPVIRDKEHVTNVSVLTSQLASLYCEVEGTPSPVITWYK--DDIQVTESSTVQIVNNGKILK------LF------------KVSAEDAGRYSCKAINIAGTSQKDFSV 1445          

HSP 10 Score: 75.485 bits (184), Expect = 3.675e-12
Identity = 184/829 (22.20%), Postives = 302/829 (36.43%), Query Frame = 3
Query:   21 GTLSTDNETYNLLSNR*-----NMEYFKTFILITLFGNLMD---RSYTLNFLITPNNATVNSK---ENLTLECVVDQQNIVYSWEYKPFHIKSNIMSKVLGFCYFEKPCVVPDSESQITISHESR-LTIHNISSDNSGYYVCRVTLLTTSEHIISLAVVTVKTP-------------PKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPF--VPNTVNA---------MINQTIDLSCEVYANPAATIQW-------------VIMQGLQIIDSSKFIAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLIHIDSNYRVPK------NSNYSINCRVTSNPPPEFIKWYKLSN--MTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILG--NKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETKD-------IVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEP-ERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQP 2306
            G L  ++E ++++S        N +   T     L  N+     +S+ LN  ++P  A V+S    E++ +        +  ++ Y P  I            +F+    + +S   I I    R L I N   DN+G Y C V      E I    V     P             PK +    N S+ +  E E  AI          P  S+   KD   + LK    +  + TIA        +   + + G   C A N AG    +  + NI+  P  +    + N ++          +IN  + L CE  A P  T+ W             +I+ G +++     I + + +  G   C A N  G       ++VL  P+I   +N  +P+      N +  + C  + NP P    W K     + D      SD   L  +    + TG Y CV +N           A S K  F    + P  P ++G  ++ +S++      + C       G P P L W+  E           V    T Q+I ++    G Y C+A N+ G+S  ++   +TV+  PSI   H ++ + +       S+S  CE    P     W   N ++ P S + +I       + R +++                     S  G+Y C A N  G    ++ L +  PP I+      ++      N V + C A  I    I W K ++ I                   +P E  + + G K   L I   +  +  NY+CVASN +G  + NF +  +  P
Sbjct: 2516 GQLLQEDEAHHMMSGGRFLQITNAQVSHTGRYTCLASNIAGDKSKSFRLNVFVSPTIAGVDSDGSPEDVIVILNSPTSLVCEAYSYPPATIT-----------WFKDGTPL-ESNRNIRILPGGRTLQILNAQEDNAGRYSC-VATNEAGEKIKHYEVKVYIPPIIKKGDLLGPGLSPKEVKIRVNSSLTL--ECEAYAI----------PSASLRWYKDG--QPLK----SDDHVTIAASGHTLQIKEAQISDTGRYTCVASNLAGEDELDFDV-NIQVPPSFQKLWEIGNMLDTGRSGEAKDVIINNPLSLHCETNAAPPPTLTWYKDGRPLTSSDRVLILPGGRVLQ----IPRAKVEDAGRYTCVAVNEAGEDSLRYDVHVLLPPVIK-GANSDLPEEVTVLVNKSTQMECSSSGNPAPRNY-WQKDGQILLEDEHHKFQSDGRSLQILNAQITDTGRYVCVAENT----------AGSAKKYFNLNVHVP--PSVIGPNHEHLSVVVNHFISLNCEVS----GFPPPDLSWLKNEEPIKPNTNVLTVPGGRTLQIIRAKISDGGDYTCIAINQAGES--KKKVSLTVHVPPSI-KDHGSQSLSIVNVREGTSVSLECESNAVPPPVITWSK-NGRMIPDSTNVEILTGGQTLHIRRAEV---------------------SDTGQYVCRAINVAGRDDKNFHLNVYVPPTIEGP--ETEVIVETISNPVTLTCDATGIPPPTITWLKNHKPI----------------ENSDPLEVHILSGGSK---LQIARPQRSNSGNYTCVASNMEGKAQKNFILFIQVPP 3244          

HSP 11 Score: 71.633 bits (174), Expect = 5.715e-11
Identity = 100/441 (22.68%), Postives = 173/441 (39.23%), Query Frame = 3
Query:  345 ISHESRLTIHNISSDNSGYYVCRVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQW-----VIMQGLQIID--SSKFIAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLIHID--SNYRVPKNSNYSINCRVTSNPPPEFIKW-YKLSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIM-KGEKFIIKCSTDRKSPGEPFPTLKW------IDVETKDIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSI 1616
            I +   L I     D++G Y C    +  +      + +TV  PP+  S   + ++N   E     + C V+ GIP P I  +  KD       ++ +N      A+     +     LE+ G   C A N AG  T+  +L  +   P      P  ++    + + LSC+    P   + W     +I      I+  S   I K+  +  G  +C A+N +G  K    + V   P+   D  SN+  P   N  +NC V  +P P  I+W  K +++  S  +    +  L          G Y CV  N         +    E++M LT+      P I    + +++  G + I+ C    ++ GEP PT+ W      I  + +  +    +  +  +      +Y+C+A N +G    R   +V V+   S+
Sbjct: 4126 ILNSGALQIAFAQPDDAGQYTCMAANM--AGSSSVSSTLTVHVPPRIQSTEVHFTVN---ENSQAVLPC-VADGIPTPAI--HWEKDG------VLIANLLGKYTAQPYGELILENVVLEDSGTYTCVANNAAGEDTRIVTLA-VHTLPTFTEL-PGDLSLNKGEQLRLSCKAVGIPLPKLTWTFNNNIIPAHFDSINGHSELVIEKVSKEDSGTYVCTAENSVGFVKAIGFVYVKEPPVFKGDYPSNWIEPLGGNAILNCEVKGDPAPT-IQWSRKGADIEISHRIRQLGNGSLAIYGTVNEDAGDYTCVAAN---------EAGMVERSMSLTLQ---SSPIITLEPVETVVDAGGRVILDC----QAAGEPQPTITWSRQGQPISWDNRLSMLPNSSLYIAAARKEDTSEYECVARNLMGSVLVRVPVIVQVHGGFSL 4533          

HSP 12 Score: 67.0106 bits (162), Expect = 1.535e-9
Identity = 93/431 (21.58%), Postives = 171/431 (39.68%), Query Frame = 3
Query:  699 LENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQWV-IMQGLQIIDSSKF--IAKIRPDMDGPLICEAKNYL-------GSTKETVQLNVLRDPLIHIDSNYRVPKNSNY------SINCRVTSNPPPEFIKWYKLSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWI--DVETK-----DIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHG 1922
            L + GF  C A++ AG   +  +  ++   P I    PN V     +   L+C V +     + W    + +++ DS++   +A +  ++    I +A  Y        GS   +V L V   P + +     +PKN ++      SI C  T  P P+ +       +  S    ++ +  L          G YAC+  N              ++T  L     PK   ++    + +  G+  +++C    K+ G P P +KW   D+E +      I   +   ++  ++    G Y C+A NE G++  R T  + V   P  I +  +  V     ++  +++  C  +G P  + +WR   + +P  S+ F +     IS  R   +F               ++L +S  G Y CEA+N+ G
Sbjct:  491 LSDEGFYDCIAVSSAGT-GRAQTFFDVSEPPPIIQL-PNNVTVTPGERAVLACLVISAVDYNLTWQRSGRDIRLADSARIRTLANLSLELRSVKIGDAGEYRCVVSSEGGSAAASVFLTVQEKPKVTV-----MPKNQSFTGGSEISIMCSATGYPKPKIVWTMNEMFIMGSHRYRMTSEGTLFIKNAVPKDAGTYACLASNAA---------GTDKQTSTLRYIEAPK--LVVEQSELLVALGDTTVMEC----KTSGIPPPQVKWFKGDLELRPSTFLSIDPLVGLLKIQETQDLDAGDYTCVAINEAGRATGRLT--LDVGSPPVFIQEPSDVAV-----EIGSNVTLPCYVQGYPEPKIKWRR-LDNMPVFSRPFSVS---FISQLRTGALFI--------------SNLWASDKGTYICEAENQFG 874          

HSP 13 Score: 55.8398 bits (133), Expect = 3.841e-6
Identity = 84/365 (23.01%), Postives = 143/365 (39.18%), Query Frame = 3
Query:  702 ENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPF-VPNTVNAMINQTIDLSCEVYANPAATIQW-------VIMQGLQIIDSSKF--IAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDP-LIHID--SNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMTDSS--AVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKW-----IDVETKDIVSDMETFQVINSESRH---NGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWR 1727
            E+ G   C A N+AG ++K+  +L +   P I     P  V+ ++N  ++LSC     PA  I W       +  + +Q ++      ++  + +  G   C A +  G   +   + V   P +  +D   ++ V +N   ++ C+  + PPP  I W K      ++    ++S    L     D   T  Y CV  NI   TT         +   LTV   P      G + +  +  +   ++C    ++ G P P + W     +  E+    S +E    ++ ES H    G+Y C+A N  G    R    + V+  PSI     N  V ++        +  CE  G P     WR
Sbjct: 3498 EDTGKYTCVATNEAGEVSKHF-VLKVLEPPHINGSEGPGEVSVIVNNPLELSCIASGIPAPKISWMKDGRPFLQTEQVQTLEGGAILRVSSAQVEDTGRYTCLASSPAGDDDKEYLVRVHVPPNIAGMDEAQDFTVLRNRQVTLECKSDAVPPP-VIMWLKNREQLQATPRVRILSGGRYLQINNADLGDTANYTCVASNIAGKTT---------REFNLTVNVPPSIGG--GPQSLVTLLNKSIALEC----RAEGVPAPRITWRKDGVVLAESHARYSILEN-GFLHIESAHVTDTGRYLCMATNVAGT--DRRRIDLQVHVPPSIAMGPTNVTVTVN-----VQTTLACEATGIPKPSVTWR 3837          
BLAST of hemicentin-1 isoform X2 vs. UniProt/SwissProt
Match: sp|O55005|ROBO1_RAT (Roundabout homolog 1 OS=Rattus norvegicus OX=10116 GN=Robo1 PE=1 SV=1)

HSP 1 Score: 102.449 bits (254), Expect = 1.684e-20
Identity = 146/617 (23.66%), Postives = 241/617 (39.06%), Query Frame = 3
Query:  849 LSCEVYANPAATIQWVIMQGLQIIDSSK---------------FIAKI------RPDMDGPLICEAKNYLG---STKETVQLNVLRDPLIHIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYK-LSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWI--DVE---TKDIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRY-GNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVM-----IRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQG-------IKKVNFEVVFKSQ--------PDPPYDLRVTDFEWDSVTINWKPGFNGGLT-QNYEILVISENFGTQNVPFYSDKNENTLTITGLYPSTPYEIRIYAVNSIG 2543
            L+C+    P  TI+W   +G + +++ K               F  +I      RPD +G  IC A+NYLG   S   ++++ +LRD      S+  V       + C+     P   I W K  S + D    +     KL      +S  G Y CV  N+     G+R+     K   +TV  +P   +   N  +++    +F  KC    ++ G+P PT  W   D E   ++  + D  T ++    +   G Y C+AEN +GK  +  +A +TV E P  + + +++ V L      ++++F CE  G P     WR  G++ +      F  +  ++ S   +SQ    LT+          T+++ S  G Y C+  N  G      +L +      +     R+    +NQ V +     + C A       I W K  + +++  Q  +    E+ V                  L I+  +  D   Y+C AS   G       I+   F V  +          P  P    VTD   ++VT+ W+P  N G T  +Y I   S   G+       +    T  I GL P+  Y   + A N+ G
Sbjct:   87 LNCKAEGRPTPTIEW--YKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPD-EGVYICVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNM----VGERE----SKVADVTVLERPSFVKRPSNLAVTVDDSAEF--KC----EARGDPVPTFGWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGK--AEASATLTVQEPPHFVVKPRDQVVALG-----RTVTFQCEATGNPQPAIFWRREGSQNL-----LFSYQPPQSSSRFSVSQT-GDLTV----------TNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQ--GPVNQTVAVDGTLTLSCVATGSPVPTILWRK--DGVLVSTQDSRIKQLESGV------------------LQIRYAKLGDTGRYTCTASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSKNTVTLLWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETFAIKGLKPNAIYLFLVRAANAYG 641          

HSP 2 Score: 73.559 bits (179), Expect = 1.147e-11
Identity = 102/468 (21.79%), Postives = 170/468 (36.32%), Query Frame = 3
Query:  987 NYLGSTKETVQLNVLRDPLIHIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMTDS------SAVLISDDAKLNFILI-----DRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKW------IDVETKDIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVL-----PFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQG--IKKVNFEV--VFKSQPDP 2312
            N LG T   ++       ++   S+  V K    ++NC+    P P  I+WYK     ++      S  ++     L F+ I      R   G+Y CV +N             S +   L   ++     ++      +  GE  +++C   R   G P PT+ W      +D + + I        +  +     GKY C+  N +G+  S+  A VTV E PS + +  N  V +       S  F CE RG P+    WR  + ++P S   ++I D   +   +++                      +   G Y C A+N  G A     L ++ PP      R + +A       V  +C+A       I W +     +L     P  S ++S+ +                    +LT+ +++  D   Y C   N  G  I K   EV  V   +P P
Sbjct:   52 NSLGYTGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPT-IEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYICVARNYLGEAVSHNA---SLEVAILRDDFRQNPSDVM------VAVGEPAVMECQPPR---GHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESK-VADVTVLERPSFVKRPSNLAVTVD-----DSAEFKCEARGDPVPTFGWRKDDGELPKSR--YEIRDDHTLKIRKVT----------------------AGDMGSYTCVAENMVGKAEASATLTVQEPPHFVVKPRDQVVALG---RTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQ------------------TGDLTVTNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPP 455          
BLAST of hemicentin-1 isoform X2 vs. TrEMBL
Match: A0A1S3HD63 (hemicentin-1 isoform X2 OS=Lingula unguis OX=7574 GN=LOC106154221 PE=4 SV=1)

HSP 1 Score: 233.032 bits (593), Expect = 1.089e-58
Identity = 249/970 (25.67%), Postives = 395/970 (40.72%), Query Frame = 3
Query:   81 YFKTFILITLFGNLMDRSYTLNFLITPNNATVNSKENLTLECVVD-----QQNIVYSWEYKPFHIKSNIMSKVLGFCYFEKPCVVPDSESQITISHES--RLTIHNISSDNSGYYVCRVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISL----WRPHYLENRGFVMCTAINDAGVLTKNTS--LLNIKYKPKIKPFVP-NTVNAMINQTIDLSCEVYANPAAT-IQWVIMQGLQIID--SSKFIAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDP---LIHIDSNYRVPKNSNYSIN--CRVTSNPPPEFIKWYKLSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPA--NSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETKDIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVF----LSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNR----HGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQPDPPYDLRVTDFEWDSVTINWKPGFNGGLTQNYEILVISENFGTQ--NVPFYSDKNENTLTITGLYPSTPYEIRIYAVNSIGRSLNSETVKCVTKRIRKFDKIQAKILSDQPKIAVIIQEPDVCANLEYTRDNGH---QWFSILHCIRFNASHINETVLLDIIRVASILDLQSGLIHLRLTFCLISRPSVCSPHRIL 2879
            +    I  TLF  +  +  T ++   P    VN   ++TL C ++     Q N+++S+  K      N  S          P       S +  + E+   L I N+S  ++G Y C+V        + + A +TV   P +     N S    +E + +   CS  GG PPP IS +  +DN   +    SS+   S  AE+   +     WR    +N     C   N A   T  T+    N+++ P +  F P N +      T  ++C V ANP  T ++W+I  G+ I +  S + +  +R    G   C A+N +GS+  T+ L+V   P   +I+I    R+  N +  +N  C V +NPP   I W K   +  +   LI       F  + R+  GMY C  +N    T  KR P    S K   L V Y+P    I  N    ++ G+   +KC+   K  G P   L W  V +    S  E   +  +    NG Y+C  +N++G+    E + V VNE P+ + Q+    +F    +S  D+   I   C  RG+P     W      I  + + F I     I    I      +T  L  R   + T ++      Y C   N      G   L   + +        +    K+A  +NQ   ++ CQ+++   +  +W +  + I       + S  E+              GL+ + LTI  +   D  NYSC+A+N  G   VN ++  + QPD P +LRV +  WDSV + W PGFNGG  Q + ++V + + G +   V F    N     ITGL+P T Y  ++Y  N++     S+ V   T  +       A    +   +    Q    C  +E  + N     +W     C          TV  + I++       S +  LR+  CL SRP VC    ++
Sbjct:   21 WMYAAIFTTLFMCVHAQDTTASWKQMPTAQAVNKGGDVTLSCEINNPGQFQINVIWSFNGKSITFNRNPFS----------PSTSDPRRSIVGANVETDFNLRITNLSLVDNGVYSCQVQ----GTDLTADAKLTVVVVPGTAVITTNNS-GTSKEGDTVFFNCSSFGGNPPPTISWF--RDNEPIQP---SSDIQYSPAAELGGSTSSKLSWRLRRADNEKMFACRVSNQANPSTPRTANTAANVQFAPVVN-FGPYNPLILREGATEQVACTVEANPQVTSVEWLI-DGVSIGNTRSVRLLDPVRLTDRGQHTCIARNIIGSSNATLTLDVQYPPKIEIINIQEGNRLTVNESSRVNLTCSVRANPPATEIVWRKNGTLQVAQPALI-------FQTVTRNRAGMYRCTAKNTL--TLSKRSPEVQESSKPAELVVQYRPGRAVI--NAHPEVVIGDPINLKCTVGDK--GIPEAQLFWERVGSTTQYSPGEDLNIPRAALDDNGSYRCTPKNKLGRGPHGEIS-VGVNEIPTWLQQNS---IFPARSVSVGDV--GIRLACRARGKPAPNIEWLKNGVPIENNGELFTIRTEEKI----IDTYSYNVTGYLEFRGTQRLTGIQVDDTANYTCLGSNHLSESRGGGKLSSSVELLVRYAPMLDRTQDKVAVDMNQTASLV-CQSQAYPAVTFRWIRAGQPITA---DGRRSFSESSGA-----------GLQTSTLTITRVSQGDLGNYSCIATNPVGETTVNIDLTVRRQPDAPTNLRVRENTWDSVLLEWTPGFNGGYDQTF-VIVKTNSSGKEEIQVGFVMQYN-----ITGLFPDTSYTFQVYGKNALKAGDYSQGVPVRTAALYVPPASDATFNGETGTLQFKSQYDQYCIRVEIEKKNAEGVLEWQEATPCAP-------PTVGDNSIKIQ-----DSTVSSLRVALCLTSRPEVCGAFSVV 912          
BLAST of hemicentin-1 isoform X2 vs. TrEMBL
Match: A0A1S3HD72 (hemicentin-1 isoform X1 OS=Lingula unguis OX=7574 GN=LOC106154221 PE=4 SV=1)

HSP 1 Score: 233.032 bits (593), Expect = 1.116e-58
Identity = 249/970 (25.67%), Postives = 395/970 (40.72%), Query Frame = 3
Query:   81 YFKTFILITLFGNLMDRSYTLNFLITPNNATVNSKENLTLECVVD-----QQNIVYSWEYKPFHIKSNIMSKVLGFCYFEKPCVVPDSESQITISHES--RLTIHNISSDNSGYYVCRVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISL----WRPHYLENRGFVMCTAINDAGVLTKNTS--LLNIKYKPKIKPFVP-NTVNAMINQTIDLSCEVYANPAAT-IQWVIMQGLQIID--SSKFIAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDP---LIHIDSNYRVPKNSNYSIN--CRVTSNPPPEFIKWYKLSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPA--NSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETKDIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVF----LSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNR----HGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQPDPPYDLRVTDFEWDSVTINWKPGFNGGLTQNYEILVISENFGTQ--NVPFYSDKNENTLTITGLYPSTPYEIRIYAVNSIGRSLNSETVKCVTKRIRKFDKIQAKILSDQPKIAVIIQEPDVCANLEYTRDNGH---QWFSILHCIRFNASHINETVLLDIIRVASILDLQSGLIHLRLTFCLISRPSVCSPHRIL 2879
            +    I  TLF  +  +  T ++   P    VN   ++TL C ++     Q N+++S+  K      N  S          P       S +  + E+   L I N+S  ++G Y C+V        + + A +TV   P +     N S    +E + +   CS  GG PPP IS +  +DN   +    SS+   S  AE+   +     WR    +N     C   N A   T  T+    N+++ P +  F P N +      T  ++C V ANP  T ++W+I  G+ I +  S + +  +R    G   C A+N +GS+  T+ L+V   P   +I+I    R+  N +  +N  C V +NPP   I W K   +  +   LI       F  + R+  GMY C  +N    T  KR P    S K   L V Y+P    I  N    ++ G+   +KC+   K  G P   L W  V +    S  E   +  +    NG Y+C  +N++G+    E + V VNE P+ + Q+    +F    +S  D+   I   C  RG+P     W      I  + + F I     I    I      +T  L  R   + T ++      Y C   N      G   L   + +        +    K+A  +NQ   ++ CQ+++   +  +W +  + I       + S  E+              GL+ + LTI  +   D  NYSC+A+N  G   VN ++  + QPD P +LRV +  WDSV + W PGFNGG  Q + ++V + + G +   V F    N     ITGL+P T Y  ++Y  N++     S+ V   T  +       A    +   +    Q    C  +E  + N     +W     C          TV  + I++       S +  LR+  CL SRP VC    ++
Sbjct:   21 WMYAAIFTTLFMCVHAQDTTASWKQMPTAQAVNKGGDVTLSCEINNPGQFQINVIWSFNGKSITFNRNPFS----------PSTSDPRRSIVGANVETDFNLRITNLSLVDNGVYSCQVQ----GTDLTADAKLTVVVVPGTAVITTNNS-GTSKEGDTVFFNCSSFGGNPPPTISWF--RDNEPIQP---SSDIQYSPAAELGGSTSSKLSWRLRRADNEKMFACRVSNQANPSTPRTANTAANVQFAPVVN-FGPYNPLILREGATEQVACTVEANPQVTSVEWLI-DGVSIGNTRSVRLLDPVRLTDRGQHTCIARNIIGSSNATLTLDVQYPPKIEIINIQEGNRLTVNESSRVNLTCSVRANPPATEIVWRKNGTLQVAQPALI-------FQTVTRNRAGMYRCTAKNTL--TLSKRSPEVQESSKPAELVVQYRPGRAVI--NAHPEVVIGDPINLKCTVGDK--GIPEAQLFWERVGSTTQYSPGEDLNIPRAALDDNGSYRCTPKNKLGRGPHGEIS-VGVNEIPTWLQQNS---IFPARSVSVGDV--GIRLACRARGKPAPNIEWLKNGVPIENNGELFTIRTEEKI----IDTYSYNVTGYLEFRGTQRLTGIQVDDTANYTCLGSNHLSESRGGGKLSSSVELLVRYAPMLDRTQDKVAVDMNQTASLV-CQSQAYPAVTFRWIRAGQPITA---DGRRSFSESSGA-----------GLQTSTLTITRVSQGDLGNYSCIATNPVGETTVNIDLTVRRQPDAPTNLRVRENTWDSVLLEWTPGFNGGYDQTF-VIVKTNSSGKEEIQVGFVMQYN-----ITGLFPDTSYTFQVYGKNALKAGDYSQGVPVRTAALYVPPASDATFNGETGTLQFKSQYDQYCIRVEIEKKNAEGVLEWQEATPCAP-------PTVGDNSIKIQ-----DSTVSSLRVALCLTSRPEVCGAFSVV 912          
BLAST of hemicentin-1 isoform X2 vs. TrEMBL
Match: A0A087U7N6 (Hemicentin-2 (Fragment) OS=Stegodyphus mimosarum OX=407821 GN=X975_20479 PE=4 SV=1)

HSP 1 Score: 209.534 bits (532), Expect = 2.919e-51
Identity = 212/839 (25.27%), Postives = 354/839 (42.19%), Query Frame = 3
Query:  471 TPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKI---ISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSL-----LNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAA-TIQWVIMQGLQIIDSSKFIAKIRPDMDGPLICEAKNYLG-STKETVQLNVLRDPLIHIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMTDSSAVLISDDAKLNFILIDRSSTGMYAC-VTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWI-DVETKDIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRH--RKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGI-KKVNFEVVFKSQPDPPYDLRVTDFEWDSVTINWKPGFNGGLTQNYEILVISENFGTQNVPFYSDKNENTLTITGLYPSTPYEIRIYAVNSIGRSLNSETVKCVTKRIRKFDKIQAKILSDQPKIAVIIQEPDVCANLEYTRDNGHQWF-----SILHCIRFNASHINETV-----LLDIIRVASILDL---QSGLIHLRLTFCLISRPSVCSPHRILENPKNENL 2903
            TPPK I+          +E+E I++ CS  GG P P I  Y  +D  + + K+    S   P S++ +V+      P   ++     C   N A  L  N  L     L + Y P+I     N +N + N    L C V A+P   +++WV    L     +  I  + PD  G   C A N +G ST+  ++L++L  P + + S   V       + C V+S PP   I+W K     D       D  ++  + +D    G Y C    + + S +  +  A    T+ + + +KP   +IL  + ++I  G  F + C+      G P P  +W  + +    ++    + +I++   H G+Y C   N +G+  S    L TV+E  SI+   K + V     D  ++ S TC  RG+P  + RW    ++I   S  F I     + +   +  FT  +I  ++    ++  L     GRY CE DN  G ++    ++      +   +RH   ++A  + +  V+ +C+ ++      +W+     +       +Y  +   +T      D++        L+I+D++ +D+  Y+C ASN  G  KK    +V K  P+ P  L + +   D VT+ W+ GFNGG + N E LV   N  T      S +++N   ITGL  +T Y  ++ AVN  G S  SE +   TK              D P+           +  +Y +D G+  F     S+    +  A  I   V     ++ +IR    + L     G   +R+  CL S  S C   + L NP  E+L
Sbjct:  114 TPPKPIA----------RERETISLTCSSEGGSPDPTIRWY--RDGVLLQGKVQKGGSRERPTSSVLDVT------PRIEDDDSVYRCVVWNRA--LADNVKLDTSVSLRVHYSPRITVGPSNPLNVLENTDAHLICNVIASPPVRSVRWVKNGNLLSNTFNHTIRSVTPDDSGIYSCIADNGIGDSTQANLELSILYGPHVKVISYQEVAAGDTLVVKCDVSSKPPSHMIQWLKEG---DPYFRQTGDTLQIEKVSVDDG--GRYICEAAASFRPSGSNVQSEATGNGTVTVRIKHKPGLAEILPVQPVAIA-GRPFTLSCTA--HPLGWPLPEYRWWKEGQQSQELTPKSNYTLISAHMSHEGRYFCQPSNALGRGGSASVYL-TVHEPASIVHPLKPQTVM---KDKEKNYSITCRARGKPKPKVRWLQNGQEISEDSGLFHITTTDTVED---NNAFTVQSILAFAGPSRRENGLTPGDRGRYTCEFDNGFGESARSEMMLRIEHSPV---VRHTYNRVAFDVGETAVL-QCKMQAYPNPQFEWFSRGRVL------DEYGNYGTNITDLG--EDIY-----AGVLSIRDMKEEDYGEYTCRASNQVGDDKKTIIRLVRKGPPERPTHLELIEVSSDKVTLRWQEGFNGGFS-NTEFLVTYLNTETGRQFNESCRSQNLCQITGLSANTEYHFKVLAVNPRGYSPYSEEMPVTTK--------------DMPR----------PSEAQYDKDTGYLTFHVELTSLKLLAKIEAKPIGSEVWILQTVIKVIRPIEKVQLSPPDEGFSDVRVKLCLESNDSWCGDAK-LANPLEEDL 874          
BLAST of hemicentin-1 isoform X2 vs. TrEMBL
Match: A0A224Z096 (Hemicentin-2-like OS=Rhipicephalus zambeziensis OX=60191 PE=4 SV=1)

HSP 1 Score: 208.764 bits (530), Expect = 5.433e-51
Identity = 224/955 (23.46%), Postives = 395/955 (41.36%), Query Frame = 3
Query:   84 FKTFILITLFGNLMDRSYTLNFLITPNNATVNSKENLTLEC----VVDQQNIVYSWEYKPFHIKSN--IMSKVLGFCYFEKPCVVPDSESQITISHESRLTIHNISSDNSGYYVCRVTLLTTSEHIISLAV-VTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRL-KIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDA---GVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAA-TIQWVIMQGLQIIDSSKFIAKIRPDMDGPLICEAKN-YLGSTKETVQLNVLRDPLIHIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMTDSSAVLISDDAKLNFILIDRSSTGMYAC-VTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETKDI-VSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQ-DFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLN-QNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQG-IKKVNFEVVFKSQPDPPYDLRVTDFEWDSVTINWKPGFNGGLTQNYEILVISENFGTQNVPFYSDKNENTLTITGLYPSTPYEIRIYAVNSIGRSLNSETVKCVTK-RIRKFDK-IQAKILSDQPKIAVIIQE--PDVCANLEYTRDNGHQWFSILHCIRFNASHINETVLLDIIRVASILDLQSGLIHLRLTFCLISRPSVCSPHRILE 2882
            F  + L T+   L+    + +  + P    V   E+L L C     +  +N++Y W     H K N  I    L   Y          E        + L        ++G+Y C++    +   + + A  VTV   P         S     E E +A+ C+  GG P P +  +   +     L +  + + P + +  ++      P   ++     C   N A   G   +  + + + Y PKI     N +N M+     L+C V ANP   +++W+    L     +  I  + P+  G   C A N  L  ++  + L+VL  P +++       +  N +I C V SNP P  I W K     D    L  D  +L+ +  +    G Y C  T  ++ S T          ++ L V +KP E +I      ++  G  F++ C    + PG P P  +W     + I +        ++  S H G+Y C   N +GK  S  +  + VNEA +II   +  ++     D  QS S TC  RG+P  +  W + ++++  S +  F+I+   NI +  +  + + LT          Q +L     G+Y C  DN  G A+    ++    P     +RH     + +  ++ ++RCQ ++      +W      +      ++Y+ +   VT  +   DL+T       L++ D++  D+ +Y+C A N  G +++   ++V KS PD P  L     + D VT+ W  GFNGG   N E +V   N  +      S ++ N  TITGL P + Y +++ AVN+ G S  SE +   TK  ++   + + A+   +  ++   +++    + A +E       QW  +   +  +   I+    L++   A+++D       +R+  CL S  S C   R+ E
Sbjct:   10 FSRWHLCTVLAVLLAARSSRSTPLEPEGRDVVEGESLRLSCNFNPALASRNLLYFWFRTNRHGKENAAISGSALDPHY--------SVEYNPAEGRYALLISRAQYDKDNGHYECKLKESGSGADVHNAAYDVTVLIAPGEP--RITPSSPTALEGEPLALTCASEGGSPDPDVDWFRGDERLPSALTRGGARDRPTAAVLTLT------PAREDDGAEFRCRITNRAMPQGSAYEARTTIRVHYAPKITVGPYNPLNVMVGTDATLTCSVLANPPVRSVRWMKRGQLLSNTYNHTIPSVTPEDSGNYACVADNGILQPSQVELHLSVLYGPRVNVLPEQEAAQGENVAIKCNVASNPEPHSILWTK---QGDGQFALQGDTLRLDRVTAE--DAGTYICQATVIMRPSATAIHTEVVGNDSVVLHVRHKPGEAEITPQGPTAVA-GRPFVLTCGA--RPPGWPRPEYRWWREGQEHIDLGHRSNHSFLSVHSSHEGRYFCQPHNALGKG-SIASVYLAVNEAATIIVPLRP-QIIRKAGD--QSQSVTCRARGKPKPQVHWTHRDKELQMSPESGFEIKTIENIEDNEVFSVQSTLTF---------QRALTPEDRGKYACLFDNGFGGAAKSEMMLRIEHPP---QVRHTYNRVAFDPGDMAVLRCQMQAYPEPTFEWVYKGRIL------ERYANYATNVT--DDGDDLYT-----GALSVADVKESDYGDYTCRAWNQVGDVQRTILKLVKKSAPDQPGRLVAVSSDSDKVTLQWTEGFNGGFG-NTEFVVTYSNVASGQTKNESCRSHNPCTITGLEPKSEYVMKVVAVNARGFSSYSEEITVQTKVNLKDMPRPLSAQFDQETGRVYFTVEQNTERLLARVEGRARGQEQWVPLAE-VEVDRPQIS----LEVANAANLVD-------IRVLLCLQSNASWCGDERLAE 898          
BLAST of hemicentin-1 isoform X2 vs. TrEMBL
Match: A0A267F8R8 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig027676g1 PE=4 SV=1)

HSP 1 Score: 208.764 bits (530), Expect = 5.641e-51
Identity = 210/903 (23.26%), Postives = 357/903 (39.53%), Query Frame = 3
Query:  189 NLTLECVVDQQNIVYSWEYKPFHIKSNIMSKVLGFCYFEKPCVVPDSE----SQITISHESRLTIHNISSDNSGYYVCRV--TLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQC-SVSGGIPPPKISMY---IVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTA----INDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQWVIMQGLQIIDSSKFIAKIRP---DMDGPLICEAKNYLGSTKETVQLNVLRDPLIHIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMTDSSAVLISDDAKLNFILID-RSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETKDI-VSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLT----------IDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLEND-DFSNYSCVASNDQGIKKVNFEVVFKSQPDPPYDLRVTDFEWDSVTINWKPGFNGGLTQNYEILVISEN--FGTQ---NVPFYSDKNENTLTITGLYPSTPYEIRIYAVNSIGRSL------NSETVKCVTKRIRKFDKIQAKILSDQPKIAVII--QEPDVCANLEYTRDNGHQWFS-----ILHCIRFNASHIN 2753
            NL L C V ++     W Y    +       VL  C+++      +S     + + +     L++  ++    GYY C      L   E I  + VVT   P         +S             C S SG  PPP+++     I K        +   + P +     S++        ++   ++CTA    +++A V      LL +K+KP I       + A+   ++ ++C     PA  +Q +   G  + +S+  +  + P      G L C A N LG+     +L+VL  P +   ++  V    + ++ C V  NPPP  + W    +    +A+  S D  L     D R   G++ C  ++I  +++G      +   + LTV Y+P  PQ        ++ GE   + C   + SPGEP P+L W+ +++  + +    + +        +G Y C A N++G S S   +L+ V++APS+  +        +      +    C  R  P    RW     ++PP                   ++FT+L           + L+ R F Q++ LR +  G Y C ADN  G ++ ++ L +   P I   +     AA +      + CQ  S S   + W +T +   L   S +  + EA             P    +   + D  +     NY+C  +N  G  +   ++V  S PDPP  L      WDSV + WKPGF+GG      + ++  N   G +   + P   D      TI GLYP T Y++   A N +G S       N+  V   T  +R     Q +  S  P    ++   +P  CA +E + D G  W +      + C+ F A  ++
Sbjct:   57 NLRLHCNVSEKPDYIFWYYS--TVPGGTDRAVLARCHWDSATCAVESGFKAWANLVVMGSGTLSLAGVARSQDGYYECEAFRDRLDAKEGIRVVVVVTASEP------SIVQSPTQLTSGNLATFTCNSGSGWNPPPRLTWLRNSIDKPAGDDLTALALYSPPAAKFGPASSVLRLTVGADDHEAVLICTAAQAELSNASVSAAQLKLL-VKHKPSIGSIA--DLTAIEGSSVPVACSAGGYPAPRLQLIGANGSVMQESASGMFTLYPVDRSQAGALACRAVNPLGTATAAFRLSVLYQPTVRTPASAVVDAGGSVTLPCSVDGNPPPAELVWR---HDATGAALPPSSDGALRLDGADPRRHAGVWHCEARSILTASSGPPTEYTASSAVVLTVRYRPGLPQA-SQPRSPLLAGESVNLTCQPSQVSPGEPAPSLAWVQIQSSPVHLPAGPSVRFSPVGLSDSGSYACRAVNDLGTSESAPVSLL-VHQAPSLTVESAPTGANAARVG-STNFRLVCRVRANPAPRLRWLLNGARMPP-------------------ELFTELAGLGDQSFEYQLTLHFRGF-QRSVLRLNDTGVYQCFADNELGNSTAEHRLEVLTQPSI---VNAFDKAAFVPGRRPALACQFISHSGFSVTW-RTPDGRTLTTGSAQ-PVEEAN----------GQPWWMTSPAPLTDGGSQPAIGNYTCQVNNSLGTVQRRLQLVAVSAPDPPERLSAAPLAWDSVRLFWKPGFDGGHPAKLRLRLVGWNASVGARMPTSPPLLVDAEVGEATIGGLYPDTAYDVVCVAENRLGESPAFGAADNNAAVVVTTGHLR---LPQVRDASWDPATGRLVFRGQPGYCARVEASSDAGATWRAPPNADAVGCLAFQADGLS 904          
BLAST of hemicentin-1 isoform X2 vs. Ensembl Cavefish
Match: ENSAMXT00000001796.2 (pep primary_assembly:Astyanax_mexicanus-2.0:20:23710211:23902940:-1 gene:ENSAMXG00000001638.2 transcript:ENSAMXT00000001796.2 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 109.383 bits (272), Expect = 1.591e-23
Identity = 146/634 (23.03%), Postives = 245/634 (38.64%), Query Frame = 3
Query:  849 LSCEVYANPAATIQWVI-------------MQGLQIIDSSKFIAKI------RPDMDGPLICEAKNYLG---STKETVQLNVLRDPLIHIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYK-LSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETK------DIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARW-RYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNV-----VMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQG-------IKKVNFEVVFKSQ--------PDPPYDLRVTDFEWDSVTINWKPGFNGGLTQNYEILVISENFGTQNVPFYSDKNENTLTITGLYPSTPYEIRIYAVNSIGRSLNSETVKCVTKRIRKFD 2600
            L+C+    P+ T++W                  + +   S F  +I      +PD DG  +C A+NYLG   S   ++++ +LRD      ++  V +     + C+     P   I W K  +N+ D    +     KL      +S  G Y CV  N+     G+R+   +E T+    ++  +   ++      ++  E    +C    ++ G+PFPT++W   + +      +I+ D  T  +        G Y CLAEN +GK  +  +A +TV+  P+   + +N+ V +      ++++F CE  G P     W R G++ +      F  +  +  S   +SQ    LTI          T ++ S  G Y+C+A N  G       L +      +     R+     NQ V     V++ C A    T  + W K    +         S  ++ +T+           L    L I+  +  D   Y+CVA++  G       +    F V  +          P  P    VTD    SVT++WK   NG    +Y I   S   G+  V         +  + GL PS  Y   + A N+ G S  S     +T  IR  D
Sbjct:   48 LNCKAEGRPSPTVEWYKDGERVETDRDNPRSHRMLLPSGSLFFLRIVHGRRSKPD-DGSYVCVARNYLGEAVSHNASLEVAILRDDFRQNPADVMVAEGEPAVLECQPPRGHPEPTISWRKDGTNINDRDERITIRSGKLMITNARKSDAGKYVCVGTNM----VGERESEIAELTVLERPSFTRRPSSVV------VLADESVEFRC----EARGDPFPTIRWRKEDGELPKGRYEILED-HTLSLQRVTPADVGSYSCLAENMVGK--AEASATLTVHVPPAFAVRPRNQVVGVG-----RTVTFQCEATGNPQPAIFWQREGSQNL-----LFSYQPPQPSSRFSVSQT-GDLTI----------TDVQRSDGGLYSCQALNIAGSVITKALLEVTDVVSDRPPPVIRQ--GPENQTVAVDGTVLLNCVATGTPTPTLLWRKDGMLV---------STHDSRITQ-----------LDSGALQIRYAKLVDTGVYTCVATSPSGEASWKAYLAVEEFGVAVQPGRPTDPNLIPSAPSKPEVTDVTRTSVTLSWKASPNGATPTSYVIEAFSHASGSSWVTLADHVKTESFILKGLKPSAVYLFLVRAANAYGLSDPSP----ITDAIRTQD 616          

HSP 2 Score: 81.2629 bits (199), Expect = 6.096e-15
Identity = 105/450 (23.33%), Postives = 172/450 (38.22%), Query Frame = 3
Query: 1041 LIHIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMTDS------SAVLISDDAKLNFILI-----DRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKW------IDVETKDIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVL-----PFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQG--IKKVNFEV--VFKSQPDP 2312
            ++   S+  V K    ++NC+    P P  ++WYK     ++      S  ++     L F+ I      +   G Y CV +N             S +   L   ++     ++      + +GE  +++C   R   G P PT+ W      I+   + I        + N+     GKY C+  N +G+  S E A +TV E PS  T+  +  V L+     +S+ F CE RG P    RWR  + ++P    +  +EDH  +S +R++        D+                G Y+C A+N  G A     L +  PP      R++ +        V  +C+A       I W +     +L     P  S ++S+ +                    +LTI D++  D   YSC A N  G  I K   EV  V   +P P
Sbjct:   31 IVEHPSDLIVSKGEPATLNCKAEGRPSPT-VEWYKDGERVETDRDNPRSHRMLLPSGSLFFLRIVHGRRSKPDDGSYVCVARNYLGEAVSHNA---SLEVAILRDDFRQNPADVM------VAEGEPAVLECQPPR---GHPEPTISWRKDGTNINDRDERITIRSGKLMITNARKSDAGKYVCVGTNMVGERES-EIAELTVLERPSF-TRRPSSVVVLA----DESVEFRCEARGDPFPTIRWRKEDGELPKGRYEI-LEDH-TLSLQRVT------PADV----------------GSYSCLAENMVGKAEASATLTVHVPPAFAVRPRNQVVGVG---RTVTFQCEATGNPQPAIFWQREGSQNLLFSYQPPQPSSRFSVSQ------------------TGDLTITDVQRSDGGLYSCQALNIAGSVITKALLEVTDVVSDRPPP 416          

HSP 3 Score: 65.4698 bits (158), Expect = 4.145e-10
Identity = 97/429 (22.61%), Postives = 157/429 (36.60%), Query Frame = 3
Query:  360 RLTIHNISSDNSGYYVC--RVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQWVIMQG------LQII-DSSKFIAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLIHI-DSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMT---------DSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKW----IDVETKD---IVSDMETFQVINSESRHNGKYKCLAENEIGKS 1568
            R+     S  + G YVC  R  L     H  SL V  ++       F  N +  +  E E   ++C    G P P IS      N   R + I+       I               + G  +C   N  G      + L +  +P      P++V  + +++++  CE   +P  TI+W    G       +I+ D +  + ++ P   G   C A+N +G  + +  L V   P   +   N  V      +  C  T NP P  I W +  +            SS   +S    L    + RS  G+Y+C   NI  S   K   A  E T  ++    P   Q   N+ +++      ++ C     + G P PTL W    + V T D      D    Q+  ++    G Y C+A +  G++
Sbjct:   92 RIVHGRRSKPDDGSYVCVARNYLGEAVSHNASLEVAILRD-----DFRQNPADVMVAEGEPAVLECQPPRGHPEPTISWRKDGTNINDRDERITIRSGKLMITNARK---------SDAGKYVCVGTNMVGERESEIAELTVLERPSFT-RRPSSVVVLADESVEFRCEARGDPFPTIRWRKEDGELPKGRYEILEDHTLSLQRVTPADVGSYSCLAENMVGKAEASATLTVHVPPAFAVRPRNQVVGVGRTVTFQCEATGNPQPA-IFWQREGSQNLLFSYQPPQPSSRFSVSQTGDLTITDVQRSDGGLYSCQALNIAGSVITK---ALLEVTDVVSDRPPPVIRQGPENQTVAVDG--TVLLNCV----ATGTPTPTLLWRKDGMLVSTHDSRITQLDSGALQIRYAKLVDTGVYTCVATSPSGEA 495          
BLAST of hemicentin-1 isoform X2 vs. Ensembl Cavefish
Match: robo1 (roundabout, axon guidance receptor, homolog 1 (Drosophila) [Source:ZFIN;Acc:ZDB-GENE-000209-3])

HSP 1 Score: 104.375 bits (259), Expect = 4.987e-22
Identity = 153/688 (22.24%), Postives = 260/688 (37.79%), Query Frame = 3
Query:  663 EVSAISLWRPHYLENRGFVMCTAINDA------GVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQWVI-------------MQGLQIIDSSKFIAKI------RPDMDGPLICEAKNYLG---STKETVQLNVLRDPLIHIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLS-NMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTY--KPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETKDI------VSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARW-RYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNV-----VMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQG-------IKKVNFEVVFKSQ--------PDPPYDLRVTDFEWDSVTINWKPGFNGGLT-QNYEILVISENFGTQNVPFYSDKNENTLTITGLYPSTPYEIRIYAVNSIGRS 2549
            + S++S+ R  + E   F++ T +           L   T L    Y P+I    P+ +     +   L+C+    P  T++W                  + +   S F  +I      +PD +G  +C A+NYLG   S   ++++ +LRD      S+  V       + C+     P   I W K   N+ D    +     KL      +S  G Y CV  N+     G+R+   +E T+    ++  +P    +L ++ +          +C    ++ G+P PT++W   E  ++      + D  T ++    S   G Y CLAEN +GK+ +  TA +TV+  P    + +N+ V +      ++++F CE  G P     W R G++ +  SSQ  +     ++S          LTI          T +     G Y+C+A N  G       L +      +     R+     NQ V     V++ CQA    T  I W K  + +++     +  +                  L    L I+  +  D   Y+C+AS   G       ++   F V  +          P  P    VTD    +V++ WKP  N G T  +Y I   S   G+            +  + GL PS  Y   + + N+ G S
Sbjct:   18 DASSVSIMRIAWSEATAFIIITLLRLGQSEVVESSLVPGTRLRLEDYPPRIVEH-PSDLIVSKGEPATLNCKAEGRPTPTVEWYKDGERVETDRDNSRSHRMLLPSGSLFFLRIVHGRRSKPD-EGSYVCVARNYLGEAVSHNASLEVAILRDEFRQNPSDVMVAAGEPAVMECQPPRGHPEPTISWKKDGVNVDDRDERITIRGGKLMITNTRKSDAGKYVCVGTNMM----GERESEIAELTVLERPSFLRRPSSQSVLVDQSVEF--------RC----EAQGDPVPTVRWRK-EDGELPKGRFEIRDDHTLKLKRLSSADAGSYTCLAENMMGKTEA--TATLTVHVPPVFAVRPRNQVVGVG-----RTVTFQCEATGSPQPAIFWQREGSQNLLFSSQPPQPSSRFSVSQ------TGDLTI----------TGIERGDAGYYSCQALNIAGSVITKALLEVTDIISDRPPPVVRQ--GPTNQTVAVDGTVVLGCQATGTPTPTILWRK--DGVLVSTHDSRLKL------------------LDTGALQIRYAKLGDTGMYTCIASTPSGEASWKAYLEVQEFGVAVQPGRPTDPNLIPSAPSKPEVTDVTRSTVSLTWKPDLNSGATPTSYIIEAFSHASGSSWQTLAEHVRTESYVLKGLKPSAVYLFLVRSANAYGLS 641          

HSP 2 Score: 69.3218 bits (168), Expect = 2.844e-11
Identity = 98/434 (22.58%), Postives = 160/434 (36.87%), Query Frame = 3
Query:  360 RLTIHNISSDNSGYYVC--RVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMY---IVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFV--PNTVNAMINQTIDLSCEVYANPAATIQWVIMQG------LQIIDSSKFIAKIRPDMD-GPLICEAKNYLGSTKETVQLNVLRDPLIHI-DSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMT---------DSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKW----IDVETKDI---VSDMETFQVINSESRHNGKYKCLAENEIGKS 1568
            R+     S  + G YVC  R  L     H  SL V  ++       F  N S  +    E   ++C    G P P IS     +  D+  +R+ I       +   +  A            G  +C   N  G      + L +  +P    F+  P++ + +++Q+++  CE   +P  T++W    G       +I D      K     D G   C A+N +G T+ T  L V   P+  +   N  V      +  C  T +P P  I W +  +            SS   +S    L    I+R   G Y+C   NI  S   K   A  E T  ++    P   Q   N+ +++      ++ C    ++ G P PT+ W    + V T D    + D    Q+  ++    G Y C+A    G++
Sbjct:  129 RIVHGRRSKPDEGSYVCVARNYLGEAVSHNASLEVAILRD-----EFRQNPSDVMVAAGEPAVMECQPPRGHPEPTISWKKDGVNVDDRDERITIRGGKLMITNTRKSDA------------GKYVCVGTNMMGERESEIAELTVLERPS---FLRRPSSQSVLVDQSVEFRCEAQGDPVPTVRWRKEDGELPKGRFEIRDDHTLKLKRLSSADAGSYTCLAENMMGKTEATATLTVHVPPVFAVRPRNQVVGVGRTVTFQCEATGSPQPA-IFWQREGSQNLLFSSQPPQPSSRFSVSQTGDLTITGIERGDAGYYSCQALNIAGSVITK---ALLEVTDIISDRPPPVVRQGPTNQTVAVDG--TVVLGC----QATGTPTPTILWRKDGVLVSTHDSRLKLLDTGALQIRYAKLGDTGMYTCIASTPSGEA 532          
BLAST of hemicentin-1 isoform X2 vs. Ensembl Cavefish
Match: robo2 (roundabout, axon guidance receptor, homolog 2 (Drosophila) [Source:ZFIN;Acc:ZDB-GENE-001019-1])

HSP 1 Score: 103.99 bits (258), Expect = 6.765e-22
Identity = 141/618 (22.82%), Postives = 237/618 (38.35%), Query Frame = 3
Query:  849 LSCEVYANPAATIQWVI-------------MQGLQIIDSSKFIAKI------RPDMDGPLICEAKNYLG---STKETVQLNVLRDPLIHIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMTDSSAVLIS-DDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISI-MKGEKFIIKCSTDRKSPGEPFPTLKW----IDVETK--DIVSDMETF--QVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERIS-QIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHG--IASLDYFLXXXXXXXXXXNIRHRKIAASLN-QNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQG---------IKKVNFEVVFKSQ-----PDPPYDLRVTDFEWDSVTINWKPGFNGG-LTQNYEILVISENFGTQNVPFYSDKNENTLTITGLYPSTPYEIRIYAVNSIGRS 2549
            L+C+    P  T++W                  + +   S F  +I      +PD +G  +C A+NYLG   S   ++++ +LRD      ++  V       + C      P   I W K     D     I     KL      +S  GMY CV  N+     G+R    ++ T+F       + P  L   I  + ++ E    +C       G+P P+++W    +DV     DI  D + +  ++  + +   G + C+AEN +GK     +A +TV   P  + + +++ V        ++ +F CE +G P     W+         SQ+    +     N R S      LTI          T ++ +  G Y C+A    G  +A     +           IR      +L    V +++CQA       I W K  + + L  +  + S+                  L +  L IK++   D   Y+CVA++  G         +K+    V  K++     P PP   +VTD   +SV+++W+PG  G  +T +Y I   S++                 TI  L P+T Y   + AVN+ G S
Sbjct:   50 LNCKAEGRPTPTVEWYKDGERVETDKDDSRSHRMLLPSGSLFFLRIVHGRRSKPD-EGAYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECVPPRGHPEPTIFWKKDKVRIDEKEDRIKIRGGKLMISNTRKSDAGMYVCVGTNM----VGERDSETAQVTVF-------ERPTFLRRPINQVVLEDEAVEFRCQVQ----GDPQPSVRWRKDDVDVARGRYDIRYDKDDYVLRIKKASAGDEGTFTCVAENRVGKL--EASATLTVRAPPQFVIRPRDQIVAQG-----RTATFPCETKGNPQPAVFWQKEG------SQNLLFPNQPQQPNSRFSVSPSGDLTI----------TGVQRADAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIIRQGPANQTLGVDGVALLKCQASGEPVPTISWRK--DGVSLLGKDPRMSL------------------LDLGSLQIKNIRLSDSGIYTCVAASSSGETSWSAFLEVKESAGVVTIKNRDESELPGPPSKPQVTDVTKNSVSLSWQPGLTGASVTTSYVIEAFSQSVSNSWQTVADHVKTTQYTIKSLRPNTIYLFMVRAVNAQGLS 608          

HSP 2 Score: 64.3142 bits (155), Expect = 9.428e-10
Identity = 103/464 (22.20%), Postives = 171/464 (36.85%), Query Frame = 3
Query:  360 RLTIHNISSDNSGYYVC--RVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVK---DNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMI--NQTIDLSCEVYANPAATIQWV-----IMQGLQII--DSSKFIAKIR---PDMDGPLICEAKNYLGSTKETVQLNVLRDPLIHIDSNYR-VPKNSNYSINCRVTSNPPPEFIKWYKLSN---------MTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETKDIVS-----------DMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNR 1637
            R+     S  + G YVC  R  L        SL V  ++       F  N +  V    E   ++C    G P P I     K   D    R+KI       S   +  A            G  +C   N  G     T+ + +  +P    F+   +N ++  ++ ++  C+V  +P  +++W      + +G   I  D   ++ +I+      +G   C A+N +G  + +  L V   P   I    + V +    +  C    NP P    W K  +            +S   +S    L    + R+  G Y C    +  S   K   A  E T  LT    P   Q   N+ + +      ++KC    ++ GEP PT+ W     KD VS           D+ + Q+ N     +G Y C+A +  G+  +  +A + V E+  ++T  KNR
Sbjct:   94 RIVHGRRSKPDEGAYVCVARNYLGEAVSRNASLEVALLRD-----DFRQNPTDVVVAAGEPAILECVPPRGHPEPTIFWKKDKVRIDEKEDRIKIRGGKLMISNTRKSDA------------GMYVCVGTNMVGERDSETAQVTVFERPT---FLRRPINQVVLEDEAVEFRCQVQGDPQPSVRWRKDDVDVARGRYDIRYDKDDYVLRIKKASAGDEGTFTCVAENRVGKLEASATLTVRAPPQFVIRPRDQIVAQGRTATFPCETKGNPQPAVF-WQKEGSQNLLFPNQPQQPNSRFSVSPSGDLTITGVQRADAGYYICQALTVAGSILAK---AQLEVTDVLTDRPPPIIRQGPANQTLGV--DGVALLKC----QASGEPVPTISW----RKDGVSLLGKDPRMSLLDLGSLQIKNIRLSDSGIYTCVAASSSGE--TSWSAFLEVKESAGVVT-IKNR 520          
BLAST of hemicentin-1 isoform X2 vs. Ensembl Cavefish
Match: robo1 (roundabout, axon guidance receptor, homolog 1 (Drosophila) [Source:ZFIN;Acc:ZDB-GENE-000209-3])

HSP 1 Score: 103.605 bits (257), Expect = 9.593e-22
Identity = 149/652 (22.85%), Postives = 247/652 (37.88%), Query Frame = 3
Query:  747 VLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQWVI-------------MQGLQIIDSSKFIAKI------RPDMDGPLICEAKNYLG---STKETVQLNVLRDPLIHIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLS-NMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETKDI------VSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARW-RYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNV-----VMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQG-------IKKVNFEVVFKSQ--------PDPPYDLRVTDFEWDSVTINWKPGFNGGLT-QNYEILVISENFGTQNVPFYSDKNENTLTITGLYPSTPYEIRIYAVNSIGRS 2549
            V    T L    Y P+I    P+ +     +   L+C+    P  T++W                  + +   S F  +I      +PD +G  +C A+NYLG   S   ++++ +LRD      S+  V       + C+     P   I W K   N+ D    +     KL      +S  G Y CV  N+     G+R+   +E    LTV  +P   +   ++ + + +  +F  +C    ++ G+P PT++W   E  ++      + D  T ++    S   G Y CLAEN +GK+    TA +TV+  P    + +N+ V +      ++++F CE  G P     W R G++ +  SSQ  +     ++S          LTI          T +     G Y+C+A N  G       L +      +     R+     NQ V     V++ CQA    T  I W K  + +++     +  +                  L    L I+  +  D   Y+C+AS   G       ++   F V  +          P  P    VTD    +V++ WKP  N G T  +Y I   S   G+            +  + GL PS  Y   + + N+ G S
Sbjct:   17 VCPPGTRLRLEDYPPRIVEH-PSDLIVSKGEPATLNCKAEGRPTPTVEWYKDGERVETDRDNSRSHRMLLPSGSLFFLRIVHGRRSKPD-EGSYVCVARNYLGEAVSHNASLEVAILRDEFRQNPSDVMVAAGEPAVMECQPPRGHPEPTISWKKDGVNVDDRDERITIRGGKLMITNTRKSDAGKYVCVGTNMM----GERESEIAE----LTVLERPSFLRRPSSQSVLVDQSVEF--RC----EAQGDPVPTVRWRK-EDGELPKGRFEIRDDHTLKLKRLSSADAGSYTCLAENMMGKT--EATATLTVHVPPVFAVRPRNQVVGVG-----RTVTFQCEATGSPQPAIFWQREGSQNLLFSSQPPQPSSRFSVSQT------GDLTI----------TGIERGDAGYYSCQALNIAGSVITKALLEVTDIISDRPPPVVRQ--GPTNQTVAVDGTVVLGCQATGTPTPTILWRK--DGVLVSTHDSRLKL------------------LDTGALQIRYAKLGDTGMYTCIASTPSGEASWKAYLEVQEFGVAVQPGRPTDPNLIPSAPSKPEVTDVTRSTVSLTWKPDLNSGATPTSYIIEAFSHASGSSWQTLAEHVRTESYVLKGLKPSAVYLFLVRSANAYGLS 606          

HSP 2 Score: 70.4774 bits (171), Expect = 1.267e-11
Identity = 98/434 (22.58%), Postives = 160/434 (36.87%), Query Frame = 3
Query:  360 RLTIHNISSDNSGYYVC--RVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMY---IVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFV--PNTVNAMINQTIDLSCEVYANPAATIQWVIMQG------LQIIDSSKFIAKIRPDMD-GPLICEAKNYLGSTKETVQLNVLRDPLIHI-DSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMT---------DSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKW----IDVETKDI---VSDMETFQVINSESRHNGKYKCLAENEIGKS 1568
            R+     S  + G YVC  R  L     H  SL V  ++       F  N S  +    E   ++C    G P P IS     +  D+  +R+ I       +   +  A            G  +C   N  G      + L +  +P    F+  P++ + +++Q+++  CE   +P  T++W    G       +I D      K     D G   C A+N +G T+ T  L V   P+  +   N  V      +  C  T +P P  I W +  +            SS   +S    L    I+R   G Y+C   NI  S   K   A  E T  ++    P   Q   N+ +++      ++ C    ++ G P PT+ W    + V T D    + D    Q+  ++    G Y C+A    G++
Sbjct:   94 RIVHGRRSKPDEGSYVCVARNYLGEAVSHNASLEVAILRD-----EFRQNPSDVMVAAGEPAVMECQPPRGHPEPTISWKKDGVNVDDRDERITIRGGKLMITNTRKSDA------------GKYVCVGTNMMGERESEIAELTVLERPS---FLRRPSSQSVLVDQSVEFRCEAQGDPVPTVRWRKEDGELPKGRFEIRDDHTLKLKRLSSADAGSYTCLAENMMGKTEATATLTVHVPPVFAVRPRNQVVGVGRTVTFQCEATGSPQPA-IFWQREGSQNLLFSSQPPQPSSRFSVSQTGDLTITGIERGDAGYYSCQALNIAGSVITK---ALLEVTDIISDRPPPVVRQGPTNQTVAV--DGTVVLGC----QATGTPTPTILWRKDGVLVSTHDSRLKLLDTGALQIRYAKLGDTGMYTCIASTPSGEA 497          
BLAST of hemicentin-1 isoform X2 vs. Ensembl Cavefish
Match: robo1 (roundabout, axon guidance receptor, homolog 1 (Drosophila) [Source:ZFIN;Acc:ZDB-GENE-000209-3])

HSP 1 Score: 101.679 bits (252), Expect = 3.850e-21
Identity = 147/651 (22.58%), Postives = 246/651 (37.79%), Query Frame = 3
Query:  756 KNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQWVI-------------MQGLQIIDSSKFIAKI------RPDMDGPLICEAKNYLG---STKETVQLNVLRDPLIHIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLS-NMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTY--KPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETKDI------VSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARW-RYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNV-----VMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQG-------IKKVNFEVVFKSQ--------PDPPYDLRVTDFEWDSVTINWKPGFNGGLT-QNYEILVISENFGTQNVPFYSDKNENTLTITGLYPSTPYEIRIYAVNSIGRS 2549
            K T L    Y P+I    P+ +     +   L+C+    P  T++W                  + +   S F  +I      +PD +G  +C A+NYLG   S   ++++ +LRD      S+  V       + C+     P   I W K   N+ D    +     KL      +S  G Y CV  N+     G+R+   +E T+    ++  +P    +L ++ +          +C    ++ G+P PT++W   E  ++      + D  T ++    S   G Y CLAEN +GK+ +  TA +TV+  P    + +N+ V +      ++++F CE  G P     W R G++ +  SSQ  +     ++S          LTI          T +     G Y+C+A N  G       L +      +     R+     NQ V     V++ CQA    T  I W K  + +++     +  +                  L    L I+  +  D   Y+C+AS   G       ++   F V  +          P  P    VTD    +V++ WKP  N G T  +Y I   S   G+            +  + GL PS  Y   + + N+ G S
Sbjct:   34 KGTRLRLEDYPPRIVEH-PSDLIVSKGEPATLNCKAEGRPTPTVEWYKDGERVETDRDNSRSHRMLLPSGSLFFLRIVHGRRSKPD-EGSYVCVARNYLGEAVSHNASLEVAILRDEFRQNPSDVMVAAGEPAVMECQPPRGHPEPTISWKKDGVNVDDRDERITIRGGKLMITNTRKSDAGKYVCVGTNMM----GERESEIAELTVLERPSFLRRPSSQSVLVDQSVEF--------RC----EAQGDPVPTVRWRK-EDGELPKGRFEIRDDHTLKLKRLSSADAGSYTCLAENMMGKTEA--TATLTVHVPPVFAVRPRNQVVGVG-----RTVTFQCEATGSPQPAIFWQREGSQNLLFSSQPPQPSSRFSVSQ------TGDLTI----------TGIERGDAGYYSCQALNIAGSVITKALLEVTDIISDRPPPVVRQ--GPTNQTVAVDGTVVLGCQATGTPTPTILWRK--DGVLVSTHDSRLKL------------------LDTGALQIRYAKLGDTGMYTCIASTPSGEASWKAYLEVQEFGVAVQPGRPTDPNLIPSAPSKPEVTDVTRSTVSLTWKPDLNSGATPTSYIIEAFSHASGSSWQTLAEHVRTESYVLKGLKPSAVYLFLVRSANAYGLS 620          

HSP 2 Score: 69.3218 bits (168), Expect = 2.813e-11
Identity = 98/434 (22.58%), Postives = 160/434 (36.87%), Query Frame = 3
Query:  360 RLTIHNISSDNSGYYVC--RVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMY---IVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFV--PNTVNAMINQTIDLSCEVYANPAATIQWVIMQG------LQIIDSSKFIAKIRPDMD-GPLICEAKNYLGSTKETVQLNVLRDPLIHI-DSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMT---------DSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKW----IDVETKDI---VSDMETFQVINSESRHNGKYKCLAENEIGKS 1568
            R+     S  + G YVC  R  L     H  SL V  ++       F  N S  +    E   ++C    G P P IS     +  D+  +R+ I       +   +  A            G  +C   N  G      + L +  +P    F+  P++ + +++Q+++  CE   +P  T++W    G       +I D      K     D G   C A+N +G T+ T  L V   P+  +   N  V      +  C  T +P P  I W +  +            SS   +S    L    I+R   G Y+C   NI  S   K   A  E T  ++    P   Q   N+ +++      ++ C    ++ G P PT+ W    + V T D    + D    Q+  ++    G Y C+A    G++
Sbjct:  108 RIVHGRRSKPDEGSYVCVARNYLGEAVSHNASLEVAILRD-----EFRQNPSDVMVAAGEPAVMECQPPRGHPEPTISWKKDGVNVDDRDERITIRGGKLMITNTRKSDA------------GKYVCVGTNMMGERESEIAELTVLERPS---FLRRPSSQSVLVDQSVEFRCEAQGDPVPTVRWRKEDGELPKGRFEIRDDHTLKLKRLSSADAGSYTCLAENMMGKTEATATLTVHVPPVFAVRPRNQVVGVGRTVTFQCEATGSPQPA-IFWQREGSQNLLFSSQPPQPSSRFSVSQTGDLTITGIERGDAGYYSCQALNIAGSVITK---ALLEVTDIISDRPPPVVRQGPTNQTVAVDG--TVVLGC----QATGTPTPTILWRKDGVLVSTHDSRLKLLDTGALQIRYAKLGDTGMYTCIASTPSGEA 511          
BLAST of hemicentin-1 isoform X2 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000010567.1 (pep scaffold:Pmarinus_7.0:GL479154:561817:685780:1 gene:ENSPMAG00000009565.1 transcript:ENSPMAT00000010567.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 90.8929 bits (224), Expect = 1.396e-18
Identity = 143/623 (22.95%), Postives = 233/623 (37.40%), Query Frame = 3
Query:  849 LSCEVYANPAATIQWVI-------------MQGLQIIDSSKFIAKI----RPDMD-GPLICEAKNYLGSTKETVQLNVLRDPLIHIDSNYR-------VPKNSNYSINCRVTSNPPPEFIKWYKLS-NMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETKDI-------VSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTK-LTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHG--IASLDYFLXXXXXXXXXXNIRHRKIAASLN-QNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKV-NELTIKDLENDDFSNYSCVASN--------------DQGIKKVNFEVVFKSQPDPPYDLRVTDFEWDSVTINWK-PGFNGGLTQNYEILVI---SENFGTQNVPFYSDKNENTLTITGLYPSTPYEIRIYAVNSIGRS 2549
            L+C+    PA T++W                  + +   S F  +I    R   D G  +C A+NYLG   E V  N   + + +I  NYR       V       + CR     P   + W +    +TD    +     KL      ++  GMY CV  N+     G+R    +E  +F   T+  K   ++      ++       +C       G+P PT++W   E  D+       + D  T +V        G Y C+ EN +GK  +  +A + V+  P  + + +++ V        QS +F CE  G P     W+         SQ+    +  +  + R S   T  LTI          T ++ S +G Y C+A +  G  +A     +           IR      +L   +V ++ CQA  + T  + W K            K S+  A +            GL+    L+IKD++  D   Y+CVA +              + G+          S   PP   RVTD   ++VT++W+ P  +G     Y I+     S++  T      +    +  T+ GL P+T Y   + A N+ G S
Sbjct:   49 LNCKAEGRPAPTVEWYKDGERVETDRDDPRSHRMLLPTGSLFFLRIVHGRRTKTDEGSYVCVARNYLG---EAVSRNASLE-VANIRRNYRSSPSAVKVAAREPAVMECRPPRGHPEPTVSWKRNGVALTDKDERVTIRGGKLMISNTQKADAGMYVCVGTNM----VGERDSEPAELAVFERPTFARKPMNLV------MLADSDAEFRCEVH----GDPPPTVRWRK-EDGDLPKGSRFEIQDDYTLRVERVGGTDEGTYTCVGENRVGK--AEASASLVVHAPPQFVVRPRDQIVAQG-----QSATFLCETTGNPPPAVFWQR------EGSQNLLFPNQPSQPSSRFSVSQTGHLTI----------TDVQRSDSGYYTCQALSVAGSILAKALLEVGDALADSPPPIIRQGPANQTLAVGSVALLACQAMGMPTPSVHWLK---------DGSKLSLGSARL------------GLQSGGTLSIKDVQLMDAGLYTCVAVSPSGETTWSAFMDVQENGVPSQPQPPEPSSLAAPPSKPRVTDVTKNTVTLSWQLPEPSGPSPILYYIIEAFRDSQSVSTSWQTVATHVTRDAFTLRGLRPNTIYLFIVRAANAHGLS 608          

HSP 2 Score: 51.9878 bits (123), Expect = 1.104e-6
Identity = 61/314 (19.43%), Postives = 117/314 (37.26%), Query Frame = 3
Query:  705 NRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQWVIMQGLQIIDSSKF---------IAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLIHIDSNYR-VPKNSNYSINCRVTSNPPPEFIKWYKLSNMT---------DSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETKDIVSDME-------TFQVINSESRHNGKYKCLAENEIGKS 1568
            + G  +C   N  G      + L +  +P      P  +  + +   +  CEV+ +P  T++W    G  +   S+F         + ++    +G   C  +N +G  + +  L V   P   +    + V +  + +  C  T NPPP    W +  +            SS   +S    L    + RS +G Y C   ++  S   K   A  E    L  +  P   Q   N+ +++  G   ++ C    ++ G P P++ W+   +K  +           T  + + +    G Y C+A +  G++
Sbjct:  196 DAGMYVCVGTNMVGERDSEPAELAVFERPTFA-RKPMNLVMLADSDAEFRCEVHGDPPPTVRWRKEDG-DLPKGSRFEIQDDYTLRVERVGGTDEGTYTCVGENRVGKAEASASLVVHAPPQFVVRPRDQIVAQGQSATFLCETTGNPPPAVF-WQREGSQNLLFPNQPSQPSSRFSVSQTGHLTITDVQRSDSGYYTCQALSVAGSILAK---ALLEVGDALADSPPPIIRQGPANQTLAV--GSVALLAC----QAMGMPTPSVHWLKDGSKLSLGSARLGLQSGGTLSIKDVQLMDAGLYTCVAVSPSGET 497          
BLAST of hemicentin-1 isoform X2 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008186.1 (pep scaffold:Pmarinus_7.0:GL476501:83598:168579:1 gene:ENSPMAG00000007342.1 transcript:ENSPMAT00000008186.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 88.1965 bits (217), Expect = 1.055e-17
Identity = 181/873 (20.73%), Postives = 316/873 (36.20%), Query Frame = 3
Query:  357 SRLTIHNISSDNSGYYVCRV----TLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWR----PHYLENRGFVMCTAINDAGVLTKNTSLLNIK-YKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQWVI------------MQGLQIIDSSKF-----IAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLIHIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSN-MTDSSAVLISDDAKLNFILIDRS-STGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGN-KIISIMKGEKFIIKCS-TDRKSP--------GEPFPTLKWIDVETKDIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLT-IDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQPDPPYDLRVTDFEWDSVTINWKPGFNGGLTQNYEILVISENFGTQNVPFYSDKNE-------NTLTITGLYPSTPYEIRIYAVNSIGRSLNSETVKCVTKRIRKFDKIQAKILSDQPKIAVIIQEPDVCANLEYTRD--NGHQWFSILHC--IRFNASHINETVL--LDIIRVASILDLQSGLIH 2819
            S L I + + ++SG YVC V     +   S  +   A V V+  P  +    + +       EF    C VSG      ++ +   D             P +  A VS     R         + G   C A  + G   ++ S+L ++   PKI       V     +   L C V  +P  TI W +             +G  I+          +   R    G   C+A N  G  +   ++NV   P I    N          ++CRV   P    I+W +    +  +    +  +  L    + +    G Y C    +Q         A+  +++ +TV    KEP ++   ++ ++ +G++  + C      SP        G+P P      VET + +S   +  + N    HNG Y CLA NE   + S  + L+ V   P  + Q K++          + +   C   G P+ +  W+Y      P  Q       R          F  L    L  R   ++        G Y C+  N  G       L+    P +  +  +  +A     +   ++C A     + I+W K  E ++ P +  +Y ++  +           T    ++ L I      D   ++C A N  G  +   ++  +  PDPP ++ V +    ++T+ W  GF+G    N  I      +  ++  +   +         N  TI  L+P+T Y  R+YA N IG S  S+ +   T+          +   D P + VI+ EP    ++  T    +GH    ++    I +  +      L   +I+R+ S  + +S L+H
Sbjct:  109 SGLVITDTTVEDSGAYVCEVWNSFGVAAVSGRLTIKAPVVVRVDPHRLKTTLSGTA------EF---SCEVSGAAGDGMVTWFHNGD-------------PPTPTASVSGAQGERLSVHAAGRSDGGTYQCVARGEGGDTAQDFSILMLEDGSPKIASSFSERV-VSPGEPFSLQCVVRGSPPPTIAWTLDGEPALSRDAPHRRGNHIVTPGDVTGYLNVTGARVTDGGVYRCQAGNAAGQAEYQARINVRGSPSIRPMRNVTAIAGREAYLHCRVIGYPY-YSIRWLREGTPLPYNHRQTVFANGTLRLADVQKGMDEGAYICSVL-VQPG-------ASPSRSLIVTV----KEPPLIQPFEMPAVTQGQRVYVPCVIVSGDSPIRITWHKDGQPLPPRLGAQVETTEFIS---SLLIPNVTQLHNGNYTCLARNE-AATVSHGSQLI-VRVPPRFVVQPKDQDGIFG-----KPVVLNCSADGYPIPKIIWKYSKGNGNPQFQPVLSGGGR----------FQPLGNGSLLLRQVQEED------GGFYLCQVSNGVGTDISKSMLLNVKIPALVTSQANVTLATRGQHS--NLQCSARGERPIIIRWEK-EETLIDPERMFRYVVYTQD-----------TGDELLSTLQILPTVRGDSGFFTCHAINSYGEDRGLIQLTVQEPPDPP-EVEVREVNARTITLRWTSGFDG----NSPITAFDIEYKNRSDTWEQKQRTKDISSTINQATIIDLHPATHYVFRVYAKNRIGASEPSKDLPITTQ----------EAAPDGPPLEVIL-EPQSSQSIRVTWKVPHGHLRNGVIRGYQIGYREAEPGSGALSQFNIVRLESTGERESCLLH 889          
BLAST of hemicentin-1 isoform X2 vs. Ensembl Sea Lamprey
Match: cntn3b (contactin 3b [Source:ZFIN;Acc:ZDB-GENE-130530-645])

HSP 1 Score: 70.0922 bits (170), Expect = 2.668e-12
Identity = 126/605 (20.83%), Postives = 222/605 (36.69%), Query Frame = 3
Query:  966 PLICEAKNYLGSTKETVQLNVLRDPLIHIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKW----IDVETKDIVSDMETFQVINSESR--HNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIA--SLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIK--WYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQPDPPYDLRVTDFEWDSVTINWKPGFNGG---LTQNYEILVISENFGTQNV---PFYSDKNENTLTITGLYPSTPYEIRIYAVNSIGRSLNSETVKCVT----------KRIRKFDKIQAK-ILSDQPKIAVIIQEPDVCANLEYTRDNG 2699
            PL+ ++   +G  +  +++     P  H    + V       + C    NP P  I W K+     S A L+  +A L          G Y C  +N+    + + K         +TV   P   Q + N    I  G+K    C    K+ G+P PT +W    + + ++   S M   ++I  E +    G Y+CLAEN+ G+ +S     V  + AP   +    R    +     Q++   CE +  P     W  GN  +              + NER+S         L+ R+ + + +      G Y C  +N  G A  +    L       +  +++   +  S     ++  CQA    ++ I   W    + I +   ++ Y + +   ++     DL           IK L+     NY+C A            ++ +  P PP  +   +    +V + WK G N     ++   +      + G  NV   P     +    T+ GL P   YE  + A+N++G S  S   + +           +R+      Q + +++ QP        PDV   L + R+NG
Sbjct:  193 PLLIKSGRSMGEYEPRIEVGF--SPKTHTLLGHTV------RLECFALGNPVP-VISWRKIDGQLPSKAYLMEANAVLEIPNFQHEDGGNYECNAENVAGKNSAQGK---------ITVYAPPHWIQPISNVEQGI--GDKLSWDC----KAGGKPPPTYRWLRNGVPLPSESRFSVMANGELILQELQPWDRGMYQCLAENKHGRIYSHGELRVKAS-APDFRSNPLKRVTPAARGG--QAV-IKCEPKAWPRPSFTWTKGNAMV--------------MDNERMS---ITQNGSLWIRSVTDEDA------GTYTCTVENHLGSANSTGRLILKESTRITVVPSLKMVSVGES-----IVWPCQASHDPSLDIVFVWSLNGQRIDMEKDAEHYQLLQGSKSQ---TSDL---------ALIKRLQLRHRGNYTCTAQTSVDNTSAWAMLMVRGPPGPPGAVVAEEATSTTVRLTWKRGSNNHSPIISYKIQARAPVASTGWLNVHTSPDIISGDSEQATVVGLTPWMDYEFTVLAINALGESEASRPSRMIKTQSAGPELAPERLGGGGGHQHELVITWQPVAPQFQYGPDVGYTLAF-RENG 728          

HSP 2 Score: 51.9878 bits (123), Expect = 9.933e-7
Identity = 62/288 (21.53%), Postives = 107/288 (37.15%), Query Frame = 3
Query:  780 KYKPKIK-PFVPNTVNAMINQTIDLSCEVYANPAATIQWVIMQG-------LQIIDSSKFIAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDP-LIHIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLS-NMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQN--IQISTTGKRKPANSEKTMFLTVTYKPKEPQILGN---KIISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETKDIVSDMETFQVINSES--------RHNGKYKCLAENEIGKSFS 1574
            +Y+P+I+  F P T + ++  T+ L C    NP   I W  + G       L   ++   I   + +  G   C A+N  G      ++ V   P  I   SN         S +C+    PPP + +W +    +   S   +  + +L    +     GMY C+ +N   +I + G+ +              K   P    N   ++    +G + +IKC    +    P P+  W   +   +V D E   +  + S           G Y C  EN +G + S
Sbjct:  204 EYEPRIEVGFSPKT-HTLLGHTVRLECFALGNPVPVISWRKIDGQLPSKAYLMEANAVLEIPNFQHEDGGNYECNAENVAGKNSAQGKITVYAPPHWIQPISNVEQGIGDKLSWDCKAGGKPPPTY-RWLRNGVPLPSESRFSVMANGELILQELQPWDRGMYQCLAENKHGRIYSHGELR-------------VKASAPDFRSNPLKRVTPAARGGQAVIKC----EPKAWPRPSFTW--TKGNAMVMDNERMSITQNGSLWIRSVTDEDAGTYTCTVENHLGSANS 470          
BLAST of hemicentin-1 isoform X2 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000002502.1 (pep scaffold:Pmarinus_7.0:GL476400:249000:366561:-1 gene:ENSPMAG00000002253.1 transcript:ENSPMAT00000002502.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 67.3958 bits (163), Expect = 2.467e-11
Identity = 168/760 (22.11%), Postives = 283/760 (37.24%), Query Frame = 3
Query:  360 RLTIHNISSDNSGYYVCRVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIA--IQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVP--NTVNAMINQTIDLSCEVYANPAATIQWV-----IMQG----LQIID--SSKFIAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLI--HIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMTDSSA----VLISDDAKLNFILIDRSSTGMYACVTQNIQISTT-GKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWID----VETKD----IVSDME-TFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSI-FEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQPDPPYDLRVTDFEWDSVTINWKPGFNGGLTQNYEILVISENFGTQNVPFYSDKNENTLTITGLYPSTPYEIRIYAVNSIG 2543
            RL +        G Y C+ T  T S+H  +   V  +T P      F++ +   +    ++   +C V+G  P     +   KDN   R      N+  + +   + +++ +     + G   C A N A      T+ L++K +P    FV    T +A ++ T  L C++  +   ++ W      I QG    +  ++  ++  I K+         C+A N  GS   T  L V+  P     ++S + VP  ++      V S   P  +KW++  N   S        ++  A L    +D +S G+Y+C   N   S T        +E+  F     + K+ Q        +  GE  +   S      G P   + W      +E  D     +S+ E T +V+N +    G Y C  +NE G+     T L T+ E P+ I   +  ++ +       ++   C+  G P     W  G++K+  S    K+    N++           T++L         SL  +  G Y+C+A+N  G A+    L +       Y  R  K   ++        C+          WYK   H V     + Y I  E  V   +      T      E T +    +D  + SC A  +   KKV     F+ +PDP        FE + +    +P     L  N EI     + G   + FY +    T+   G   S  Y  +  AVN +G
Sbjct: 5082 RLIVMKTDRSEGGVYTCKATNETGSDHCSTQLEVKERTIPPD----FDEKLESLEVTAGLSADFECHVTGSAP---FRVTWTKDNRDIR---SGGNYKFTFLDNTAHLTILKADK-GDIGTYTCKAANAA-GSAACTADLSVKDRPVAPSFVRKLKTTDAALSSTAHLECKLSGSVPISVVWFKGSEEIAQGERYKMTFVENVATLEIQKVVASDAFSYTCKATNVSGSEASTADLKVIEPPSFTKTVESQHVVP-GASVEFKSSV-SGTEPLTVKWFRDDNELMSGRRCKITFLNGVATLKLDDVDAASAGVYSCQVSNAAASETCSANLTVKAERANF---RRRVKDSQ--------VSNGETAVFSTSFS----GTPEIVVFWYKQNNLIEESDKYRVAISEGESTLEVLNVDKSDEGIYTCKIQNEAGEDSCLAT-LATL-EPPNFIKGLEPTEIVVG-----STVHLECQVAGTPQIRVSWYKGSQKL-KSGDKCKMAFKNNLA-----------TLELR--------SLTKADTGDYSCKAENDFGSATSLASLSVSERKNPPYFTRKLKDWQAVEGTAATFDCRVTGTPPFSFIWYK---HGVELSSGEDYVISAEENVATLDIHNVKLT---HAGEYTCRVF--NDVDSASCSAKLEAKDKKV--PPSFEKKPDPVEVTAGESFELECLIAGSRPIRISWLKNNREI----RSVGNYKLFFYDNAARLTVLKAGKEDSGTYVCK--AVNEVG 5769          

HSP 2 Score: 63.5438 bits (153), Expect = 3.491e-10
Identity = 146/692 (21.10%), Postives = 256/692 (36.99%), Query Frame = 3
Query:  363 LTIHNISSDNSGYYVCRVT-LLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPN---TVNAMINQTIDLSCEVYANPAATIQW---------------VIMQGLQIIDSSKFIAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLIHIDSNYRVPK-----NSNYSINCRVTSNPPPEFIKWYK----LSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWI--DVETKDIVSDMETFQ-------VINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDL----PQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQPDPP 2315
            L I N+++++   Y C V+  +       SL V   K PP   SF    +       E   + C V+G  PP KIS   +KDN   R     +N+  S +     +++ +    ++ G  +C A N+AG  T + + + IK + ++ P       ++N    +  +L C V  +    + W               + M+ +  +     I K+     G   C A N +GS   + ++ V +D  +      ++ K     +S   ++CRV    P   + W K    + +      +   + A L  + +D+S  G Y C   N+    +     +  E   FL       EPQ        ++ G +   +C+    S  EP   +KW   + E +  +S   +F+       V   +    G+Y C   N  GK+    +  V   + P+        K F   SDL      S SF C F G P     W   + +I        +  H+     +++ +  + T+D+ +   S         +G Y C+  N  G  +    L +  PP    ++   +     +   V   CQ      + + WYK ++ +       KY             + +F     V  L +K+ E  D   YSC A N  G  K +  +  K +  PP
Sbjct: 6114 LEILNLATNDEEEYSCTVSNDVGKISCAASLEVKERKNPP---SFDQQLAPASVTAGEPADLGCHVTGS-PPFKISW--LKDNKEIR---PGTNYKFSFVDNTPLLTILKTDKRDS-GVYVCRAANEAGYDT-SCAPVTIKER-RVAPTFDQKLESLNVTTGERAELECHVTGSVPIKVTWAKDNREIRAAGNYRMIFMENIARLT----ILKVGKLDSGIYTCAASNDVGSDVTSTEITV-KDQAVPPSFVRKLKKTDAILHSTAQMDCRVAGTVPI-LVAWSKNGVEIVDGDKYRCLFEENTASLEILKVDKSDDGSYTCTATNVAGRDSCSTTLSIIEPPSFLM----KMEPQ-------EVLSGSRATFQCAV---SGTEPL-LVKWYKGNQELRTDISHSISFRDSVALLIVEKVKLAFAGEYSCQVSNSAGKTSCSASLFV---KEPA--------KFFSKPSDLDVKAGDSASFICSFVGTPKIAVNWYKEDNQI--------VAGHKF----QVTTVEGQSTLDILATEISD--------SGNYTCKIQNDSGQDNCTALLKVLDPPYFVKSLEPMEGTFGTS---VYFECQVAGAPEIRVTWYKGSQKL----SGAKY-------------KTMFQHNTAV--LEVKNFEKADVGTYSCEAENSLGSAKTSASLAGKERKHPP 6719          

HSP 3 Score: 60.077 bits (144), Expect = 4.002e-9
Identity = 119/563 (21.14%), Postives = 204/563 (36.23%), Query Frame = 3
Query:  699 LENRGFVMCTAINDAGVLTKNTSLLNIKYKPK-IKPFVPNTVNAMINQTIDLSCEVYANPAATIQW------VIMQG------------LQIIDSSKFIAKIRPDMDGPLICEAKNYLGSTKETVQLNV-----LRDPLIHIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYK----LSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIIS--IMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETKDIVSD----------METFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSI-FEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQG 2264
            L + G  +CTA ++AG  +  +S+  +K  P  ++   P    A     + L C +  +P   + W      V   G            L+I DS         +      CEA+N +G+   T  ++V      + PL     +  V  NS+ ++   +  +PP + +KW K    L  +    A L +  A L       +  G Y+C  +N      GK +              K KEP     K I+  + +G    +  +     P     T+ W   + K I  D               ++ +E  H G+Y C A+NE G      +A+++V E  + + + ++  V +      +   F C   G      RWR  +E +  SS+ FK+    N+++ ++      ++ D +               G Y CE  N  G +  +  L I    +    +R  K    +     ++ C+    S + + W+K  E I     S K+ I FE    K E                I +    D   Y+C A N  G
Sbjct: 4314 LADTGDYVCTASSEAGSESTASSV-EVKADPAGLRALQPEVKEA--KGLVHLDCSLKGSPVIKVTWFKNDNEVKADGRVQLSFVNNVAVLEIRDS-------ETEDSADYTCEAQNEVGTVSCTCAVSVKEIPSFKKPL----ESMEVRGNSDVTLEVELDGSPPFD-VKWVKNARELPKIDKYRAKLENTTATLRIGNFSAADVGQYSCKVKN----NVGKDESH---------ANIKLKEPPKFTKKFINSEVSEGSSATLNATLIGSQP----MTIAWSKDKLK-IAEDENHSISYKDNHAILTIVKAEKSHAGEYTCDAKNEAGAQSC--SAVLSVKEPATFVDKMESVNVTVG-----EVAFFECRVAGSVPITVRWRKDHELL-KSSKKFKMTFTNNVASLKL------MSTDKFD-------------TGEYYCEVQNDGGSSICNATLTILDRIVPPTFVRKLKNGDCVLHAKAVLECKLAGSSPLTVSWFKGVEEIS---DSDKFKISFEDNTAKVE----------------ILNASQVDEGTYTCKADNAAG 4797          

HSP 4 Score: 58.151 bits (139), Expect = 1.524e-8
Identity = 152/692 (21.97%), Postives = 248/692 (35.84%), Query Frame = 3
Query:  363 LTIHNISSDNSGYYVCRVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISM-YIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPK-IKPFVPNTVNAMINQTIDLSCEVYANPAATIQWVIMQGLQIIDSSKFIAKIRPDMD------------GPLICEAKNYLGSTKETVQLNVLRDPLIHIDSNYRVPKNSNYSI------NCRVTSNPPPEFIKWY----KLSNMTDSSAVLISDD-AKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETKDI----------VSDMETFQVINSESRHNGKYKCLAENEIGK--SFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSR-----TFSQQTSLRS------SPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNV----------VMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQG 2264
            L +  +    +G Y C+V+   ++      A + VK P K  S   +  +       FI   CS  G    PKI++ +  +DN I    +    F  +T+   S + +     + + G   C   ND+G     T+LL +   P  +K   P  +      ++   C+V   P   + W   +G Q +  +K+    + +              G   CEA+N LGS K +  L        H  S  R+ KN    I      + RVT  PP   + W+    +LS  +D   +   D+ A L+   +  S  G Y C   N   S +   K   +E+        KP   Q L  + +  + G+   ++C      P + F    W+  + KDI            +     +   +   +G+Y C A N+IG   S + ETA+               RKV   ++  P  I    E +G  L                      D R   ++ I+  +TK   DL++      +F    +L S      S +G Y C+A+N  G A+    L          NI+ RK A + ++ V          +   C+      + I W K  +  V PF     +  E + T                 L I  +   D   Y+C A N+ G
Sbjct: 6495 LIVEKVKLAFAGEYSCQVS--NSAGKTSCSASLFVKEPAKFFSKPSDLDVKAGDSASFI---CSFVG---TPKIAVNWYKEDNQI----VAGHKFQVTTVEGQSTLDILATE-ISDSGNYTCKIQNDSG-QDNCTALLKVLDPPYFVKSLEP--MEGTFGTSVYFECQVAGAPEIRVTW--YKGSQKLSGAKYKTMFQHNTAVLEVKNFEKADVGTYSCEAENSLGSAKTSASLAGKERK--HPPSFSRMLKNMQQVIGDCAKFDARVTGTPPF-HVSWFLGDSELS-YSDKHVISFEDNVASLSIFSLKVSDAGEYTCKVSNEDGSISCSAKLDVTERKT------KPTFDQKL--EPVEAVVGDPAELECHLTGSLPIKIF----WMK-DKKDIRPGPNCKMTFFDNTPHLTIYKLDKSDSGEYTCKAVNDIGSDNSCTCETAIA-------------ERKVAPMFTKKPLEILDEEEGKGVKL----------------------DSRVSGSQPIAITWTKDDKDLHAHPGRELSFKNNIALMSLKSTKVSDSGLYVCKAENEAGTATFKVTL----------NIKERKHAPNFDKKVQSLTVVEGDTLSASCRVSGSEPLQIMWLKDRKE-VQPFGKVSIAYSEGDCT-----------------LEISQVTKADAGEYTCKAVNEAG 7088          

HSP 5 Score: 55.0694 bits (131), Expect = 1.217e-7
Identity = 145/685 (21.17%), Postives = 244/685 (35.62%), Query Frame = 3
Query:  342 TISHESRLTIHNISSDNSGYYVCRVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQE---QEFIAIQCSVSGGIPPPKISM-YIVKDNAIKRLKIISSNF-PNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQW-----VIMQGLQIIDSSKF------IAKIRPDMDGPLICEAKNYLGSTKETVQLNVL--RDPLIHID--SNYRVPKNSNYSINCRVTSNPPPEFIKWYKLS-NMTDSSAVLISDDAKLNFILIDRSSTG---MYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKI--ISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETKDI----------VSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFT--------CEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQG 2264
            T  H   L I  +   + G Y C+V  +   +   ++  VT+  PP      F+K++  +QE    E++  Q  V+G  P   +S+ +      +K     S +F  N  + E+S +         + G   C   N AG+ T +  L        +K   P +V         L CEV   P     W     +I  GL      K       +  ++    G  +C+A+N +GS      L V   ++P +      + +  +      +CR T  PP   + W+K    +T      +S +  + F+ I   +T     Y+C   N      GK   A S     L V  + K P     ++   S+  GE   + C       G P   + W+  + K+I          V +     ++ ++ R +G Y C A NE G  +    A VT+ E          R+V  ++    +S++ T        C   G    +  W   N +I  +       ++R I  E I+    +LTI    +  S          G Y C A N  G       + +K   +    +R  K   ++  +   + C+      + + W K    IV      KY     E T                 L I  ++  D  +Y+C A+N  G
Sbjct: 5836 TSDHTVNLEILELGIADGGNYTCKVNNVAGKDSCSAILRVTIAEPP-----GFSKTLE-YQEVLLGEYVRFQSIVTGTEP---LSVKWFADSKELKPTDNCSISFRGNRALLELSQVD------SASAGEFTCQVANAAGIETCSAKLF-------VKVSEPGSVR--------LECEVAGTPVIKTSWFKDDVLITSGLNRKPVQKLNLAILELRNVQYSESGEYVCKAENDIGSATSVAMLTVTERKNPPVFTRKLKDVQGVEGGAAMFDCRATGTPPFA-VAWFKHGKEITPGEKYRMSFEENVVFLEILNLATNDEEEYSCTVSN----DVGKISCAAS-----LEVKER-KNPPSFDQQLAPASVTAGEPADLGCHV----TGSPPFKISWLK-DNKEIRPGTNYKFSFVDNTPLLTILKTDKRDSGVYVCRAANEAG--YDTSCAPVTIKE----------RRVAPTFDQKLESLNVTTGERAELECHVTGSVPIKVTWAKDNREIRAAG------NYRMIFMENIA----RLTILKVGKLDS----------GIYTCAASNDVGSDVTSTEITVKDQAVPPSFVRKLKKTDAILHSTAQMDCRVAGTVPILVAWSKNGVEIV---DGDKYRCLFEENTA---------------SLEILKVDKSDDGSYTCTATNVAG 6424          

HSP 6 Score: 55.0694 bits (131), Expect = 1.501e-7
Identity = 158/747 (21.15%), Postives = 274/747 (36.68%), Query Frame = 3
Query:  363 LTIHNISSDNSGYYVCRVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQW------------VIMQGLQIIDSSKFIAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRD----PLIHIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYK-LSNMTDSSAVLIS---DDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVET------------KDIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPN--------GRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMI----------RCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQPDPP-YDLRVTDFEWDSVTINWKPGFNGGLTQNYEILVISENFGTQNVPF 2450
            L I N S+ + G Y C+V      +   S A + +K PPK      N  ++   E     +  ++ G  P   +++   KD    +LKI      + +  +  AI         + G   C A N+AG  +  +++L++K +P        +VN  + +     C V  +   T++W              M     + S K ++  + D  G   CE +N  GS+     L +L        +    N     ++   + C++  + P   + W+K +  ++DS    IS   + AK+  +   +   G Y C   N   + +   K    E   F  V  KP+E   L   ++S            T   S  +P  ++KW    T            K++ + +E  +V   +S   G+Y C   N +G      +A + V E     T+ ++  V     +L  + SF C F G P    RW   +E++  S + FKI      S+  +  I T   +  YS   S +    S  N        G Y C A N  G +S +  LI          ++ RK+A + +Q +  +           C       + I W K N+ I        Y I   + T                 L +   +  +   Y+C A+N+ G    + ++  K +  PP +D ++   E              GL+ ++E  V      T + PF
Sbjct: 4493 LRIGNFSAADVGQYSCKVKNNVGKDE--SHANIKLKEPPKFTKKFINSEVS---EGSSATLNATLIGSQP---MTIAWSKD----KLKIAEDENHSISYKDNHAILTIVKAEKSHAGEYTCDAKNEAGAQS-CSAVLSVK-EPATFVDKMESVNVTVGEVAFFECRVAGSVPITVRWRKDHELLKSSKKFKMTFTNNVASLKLMSTDKFDT-GEYYCEVQNDGGSSICNATLTILDRIVPPTFVRKLKNGDCVLHAKAVLECKLAGSSPLT-VSWFKGVEEISDSDKFKISFEDNTAKVEILNASQVDEGTYTCKADNAAGTDSCWAKVKVMEPPSFKEV-IKPQEA--LPGAVVSF-----------TSVVSGTDPL-SVKWFKDGTELLPSRNSRISFKNLSAVIELDKV---DSTAAGEYSCQVSNAVGTETC--SATLFVKEPAKFETKLQSTNV-----NLGDTASFNCTFTGTPEITVRWFRKDEELQDSDK-FKIVTTATSSSLEVLNI-TSDDVKPYSCRISNEAGEDSCINSLSVQADAGEYVCTAANEAGTSSCNANLI----------VKERKVAPTFDQKLQPVEVTAGDPSEFECHVTGSLPIKITWSKNNKEI---RSGGNYRITFLDNTP---------------RLIVMKTDRSEGGVYTCKATNETGSDHCSTQLEVKERTIPPDFDEKLESLE-----------VTAGLSADFECHV------TGSAPF 5151          
BLAST of hemicentin-1 isoform X2 vs. Ensembl Sea Lamprey
Match: pxdn (peroxidasin [Source:ZFIN;Acc:ZDB-GENE-140415-2])

HSP 1 Score: 62.7734 bits (151), Expect = 5.422e-10
Identity = 87/412 (21.12%), Postives = 148/412 (35.92%), Query Frame = 3
Query: 1071 PKNSNYSINCRVTSNPPPEFIKWYKLSN---MTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTY--KPKEPQ-ILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETKDIVSDMETFQVINSESRH--------NGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQG 2264
            P N+ Y + CR   NP P  I W   SN   M +     +  D  L       +  G Y C+ +N+          A   K   + + Y   P  P  ++      ++ GE   ++CS      G P P + W   +++ + +D   F V  S   H         G+Y C A N   +   R +A + +  AP  I   +++ V   ++     +   CE +G+PL    W     ++P              S+ R++ + +  T+ +       Q        G++ C+A N  G+      L +       +++    I     ++ V I C A+      + W K   HI    +S K+ +  AE                   L I+D    D   Y C+A N  G
Sbjct:  196 PGNTVY-LRCRAEGNPKPAII-WLHNSNEIAMAEDERRNLLQDGTLMIRDTRHTDQGTYQCMARNV----------AGEVKAPEVVLRYFDVPAPPTFVIQPHDTEVLVGESVTLECSAG----GHPAPRVSWTRDDSEQLPAD-PRFSVTASGGLHVERAGPGDEGRYTCHASNN--QDSIRASATILLKSAPQFILAPEDQVVTEGHT-----VDLHCEAQGRPLPVIAWTKAGSQLP--------------SDRRLT-VLSSGTLRIARVALHDQ--------GQFECQAVNVVGVRRAATQLTVLPRVTPVFSVTPSDITTDSGRD-VRIACSAQGEPQPSLMWAKDGVHIT---ESGKFHV-SAE-----------------GYLEIRDAGPADQGRYECIARNSIG 538          

HSP 2 Score: 62.7734 bits (151), Expect = 5.514e-10
Identity = 67/307 (21.82%), Postives = 110/307 (35.83%), Query Frame = 3
Query:  705 NRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFV--PNTVNAMINQTIDLSCEVYANPAATIQWVIMQGLQIIDSSKF---------IAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDP-LIHIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMTDSSAVL-ISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKW------IDVETKDIVSDMETFQVINSESRHNGKYKCLAENEIGKS 1568
            ++G   C A N AG +     +L     P    FV  P+    ++ +++ L C    +PA  + W      Q+    +F         + +  P  +G   C A N   S + +  + +   P  I    +  V +     ++C     P P  I W K  +   S   L +     L    +     G + C   N+     G R+ A       LTV  +      +    I+   G    I CS      GEP P+L W      I    K  VS     ++ ++     G+Y+C+A N IG +
Sbjct:  248 DQGTYQCMARNVAGEVKAPEVVLRYFDVPAPPTFVIQPHDTEVLVGESVTLECSAGGHPAPRVSWTRDDSEQLPADPRFSVTASGGLHVERAGPGDEGRYTCHASNNQDSIRASATILLKSAPQFILAPEDQVVTEGHTVDLHCEAQGRPLP-VIAWTKAGSQLPSDRRLTVLSSGTLRIARVALHDQGQFECQAVNV----VGVRRAATQ-----LTVLPRVTPVFSVTPSDITTDSGRDVRIACSAQ----GEPQPSLMWAKDGVHITESGKFHVSAEGYLEIRDAGPADQGRYECIARNSIGHA 540          
BLAST of hemicentin-1 isoform X2 vs. Ensembl Nematostella
Match: EDO31942 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SWF5])

HSP 1 Score: 72.4034 bits (176), Expect = 6.424e-13
Identity = 90/369 (24.39%), Postives = 152/369 (41.19%), Query Frame = 3
Query:  498 FNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDA--GVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQWVIMQGLQIIDSSKF-IAKIRPDMDGPLICEAKNYLGSTKETVQLN----VLRDPLIHIDSNYRVPKNSNYSINCRVTSNP-PPEFIKWYKLSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETKDIVSDME-----TFQVINSESRHN-GKYKCLAENEIG 1562
             ++S +V ++++F +I CS SG  P P ++ ++ K          +S  P +     + +SL +       G   C A ND   G +T++ ++ N++Y P+I P    TV A   +TI L+C V  NP  ++ W      Q    + F  A       G  +C A N +    +T  ++    V   P I  +S Y+    +  S+     S+  P   + W +   +   ++V  SD        + RS  G Y C   N   S T          T+ +TV YKP+      N   S    +   ++C    K+ G P P + W     + I + +        QV  ++   + G+YKC A N +G
Sbjct:    8 LDRSRDVAEDKDF-SITCSASG-RPAPNVT-WVNK----------TSGSPVAHGTGSAILSLLKIQ-RHQAGVYQCQATNDVKRGAITQDFTI-NVQYPPEITPIQNTTVRA--GETITLTCAVAGNPTPSVSWSKAGSGQSTGGNVFNEAGATKAETGQYVCTAVNIVSGNTQTKTVSTYVIVKYKPSIIANSPYQTVNETTGSLKLSCISDGYPAPMVTWSRDGVVVSPTSVYYSDS-------VTRSEAGTYTCTATNEMGSDT---------NTVQVTVNYKPEMASSTTN--TSSWNRKVLSLQC----KARGVPLPQITWYKPGGQPITAGVSPISGGQLQVTTTQDSGDYGQYKCRASNLLG 337          

HSP 2 Score: 64.6994 bits (156), Expect = 1.655e-10
Identity = 104/502 (20.72%), Postives = 184/502 (36.65%), Query Frame = 3
Query: 1050 IDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETKDIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRET--ALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQPDPPYDLRVTDFEWDSVTINWKPGFNGGLTQ--NYEILVISENFGTQNVPFYSDKNENTLTITGLYPSTPYEIRIYAVNSIG 2543
            +D +  V ++ ++SI C  +  P P  + W  ++  + S     +  A L+ + I R   G+Y C     Q +   KR     + T  + V Y    P+I   +  ++  GE   + C+      G P P++ W    +    +    F    +     G+Y C A N +  +   +T    V V   PSII        + + ++   S+  +C   G P     W      + P+S  +                                 S+  S  G Y C A N  G  +    + + Y P +  +  +    +S N+ V+ ++C+A  +    I WYK     +            A V+           G ++   T +D  + D+  Y C ASN  G       V     P PP  + V++ E  S+ +NW P  + G +   +Y++ V          P         + I+GL  +T Y +++ A N +G
Sbjct:    8 LDRSRDVAEDKDFSITCSASGRPAPN-VTW--VNKTSGSPVAHGTGSAILSLLKIQRHQAGVYQC-----QATNDVKRGAITQDFT--INVQYP---PEITPIQNTTVRAGETITLTCAVA----GNPTPSVSWSKAGSGQ-STGGNVFNEAGATKAETGQYVCTAVNIVSGNTQTKTVSTYVIVKYKPSIIANSP----YQTVNETTGSLKLSCISDGYPAPMVTWSRDGVVVSPTSVYYS-------------------------------DSVTRSEAGTYTCTATNEMGSDTNTVQVTVNYKPEMASSTTN---TSSWNRKVLSLQCKARGVPLPQITWYKPGGQPIT-----------AGVSPIS--------GGQLQVTTTQD--SGDYGQYKCRASNLLGSVGRLIYVTQWFSPGPPI-IIVSNIEASSIKVNWAPPSSDGGSPVIDYKVEVDPAK------PPREGVAGGPVVISGLTKNTEYTVKVTARNVVG 425          

HSP 3 Score: 63.1586 bits (152), Expect = 4.879e-10
Identity = 76/330 (23.03%), Postives = 123/330 (37.27%), Query Frame = 3
Query:  789 PKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQWV-IMQGLQIIDSSK----FIAKIRPDMDGPLICEAKNYL--GSTKETVQLNVLRDPLIHIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPK----EPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETKD--IVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSII-----TQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARW 1724
            P   P +  + +   ++   ++C     PA  + WV    G  +   +      + KI+    G   C+A N +  G+  +   +NV   P I    N  V      ++ C V  NP P  + W K  +   +   + ++          ++ TG Y C   NI    T  +       + ++ V YKP      P    N+    +K     + C +D    G P P + W    ++D  +VS    +   +      G Y C A NE+G   +  T  VTVN  P +      T   NRKV          +S  C+ RG PL +  W
Sbjct:    2 PAFNPPLDRSRDVAEDKDFSITCSASGRPAPNVTWVNKTSGSPVAHGTGSAILSLLKIQRHQAGVYQCQATNDVKRGAITQDFTINVQYPPEITPIQNTTVRAGETITLTCAVAGNPTPS-VSWSKAGSGQSTGGNVFNEAGAT------KAETGQYVCTAVNIVSGNTQTKT-----VSTYVIVKYKPSIIANSPYQTVNETTGSLK-----LSCISD----GYPAPMVTW----SRDGVVVSPTSVYYSDSVTRSEAGTYTCTATNEMGSDTN--TVQVTVNYKPEMASSTTNTSSWNRKV----------LSLQCKARGVPLPQITW 294          
BLAST of hemicentin-1 isoform X2 vs. Ensembl Nematostella
Match: EDO32297 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SVH5])

HSP 1 Score: 65.0846 bits (157), Expect = 2.085e-11
Identity = 62/247 (25.10%), Postives = 105/247 (42.51%), Query Frame = 3
Query:  564 GIPPPKISMYIVKD---NAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQWV-----IMQGLQI----IDSSKFIAKIRPDMDGPLICEAKNYLGSTKETVQLNVLR-DPLIH---IDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQN 1256
            G+P P +S     D   + +  +  +S++  N TI +             + G  +CTA N  GV +  ++L    +K    PF    V  +++  ++++C V   P  +I W+     + Q + +    +  + ++  I     G  +C A N LG+   T  LNVL  D L H   I  N     +++ SI+C  T NP P  I W +   + + S  +    + L  +    S TG Y CV  N
Sbjct:    9 GLPKPSVSWTRAGDKLSHWLASMGRVSTSNGNVTITQAEP---------NDAGMYLCTARNLLGVASARSTLY---FKGMFHPF---DVITLLSSNVEVTCPVRGFPLPSITWLKDGQPLKQSMTVKVNNLTGTLYLLSILERRAGLYMCVATNSLGAATATTTLNVLHYDSLSHALTIGDNLNTLISTSVSIHCNATGNPVPS-ITWSRSGALIEPSKRIKIMGSTLVIVGTLWSDTGSYECVASN 239          
BLAST of hemicentin-1 isoform X2 vs. Ensembl Nematostella
Match: EDO32296 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SVH4])

HSP 1 Score: 62.003 bits (149), Expect = 8.361e-10
Identity = 77/326 (23.62%), Postives = 132/326 (40.49%), Query Frame = 3
Query:  711 GFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQWV------IMQGLQIIDSSKFIAK-IRPDMDGPLICEAKNYLG----STKETVQLNVL-RD-PL-------IHIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSN-MTDSSAVLISDDAKLNFILIDRSSTGMYAC----------VTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETKDIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVT 1595
            G   CTA N  G    N++ +       +K  V + +  + +  + + CEV   P  ++ WV      + +G  I D+   + +  RP   G   C A N  G    S++ T++  V  RD PL         I  N  V   S  +I+C  T  P P  I W K    +  + + +   +  L    +  S  G++ C          V+ NI++ TT K + ++           KP + +I G   ++ + G    + CS    + G P P ++W++   K I        + N  S  +G Y C+A N +G++ +     VT
Sbjct:  103 GRYTCTARNAFGKDNANSTTIPPGDGSPVKVSVGDNITVITDTPLLIDCEVSGIPFPSVVWVKDGRVLMGEGYVIFDNGTLVLRRARPADSGRYTCTANNSRGKDHISSQPTIERPVAPRDIPLGDGSPTIARIADNATVLTGSRLTIDCVATGVPKPSII-WRKDGRELIGAGSYMAPGNGSLLIQQVAASHHGVFTCTATNPVGEDSVSSNIRVRTTDKVEKSD----------MKPVDVRIGGQ--VTSLSGVDITLNCS----ARGLPPPDIEWMN-GGKMITVQGPRLVLRNVMSSDSGNYTCIASNVVGRASASTMVNVT 410          

HSP 2 Score: 57.7658 bits (138), Expect = 1.588e-8
Identity = 79/372 (21.24%), Postives = 138/372 (37.10%), Query Frame = 3
Query:  711 GFVMCTAINDAGVLTKNTSLL----NIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQWVI----MQGLQIIDSSKFIAKIRPDMDGPLICEAKNYLG---STKETVQLNVLRDPLIHIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKI---ISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETKDIVSDMETFQVINSE-------SRHNGKYKCLAENEIGK-SFS-----RETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKI 1745
            G  +CTA+N  G++ K+T+ +    N  +K KI     N +  M  + + L C     P  TI W      M+     + S  + K+     G   C A+N  G   +   T+         + +  N  V  ++   I+C V+  P P  + W K   +      +I D+  L       + +G Y C   N +       +P          +      P I   +I    +++ G +  I C     + G P P++ W   + ++++         N         + H+G + C A N +G+ S S     R T  V  ++   +  +   +   LS  D    I+  C  RG P  +  W  G + I
Sbjct:   15 GRYVCTAVNAIGIIHKSTNYIVWYENRPFKTKIG----NNLTTMKGRKLTLICHATGRPRPTITWFRGGEEMRTKVTNNGSLILEKVSSSDIGRYTCTARNAFGKDNANSTTIPPGDGSPVKVSVGDNITVITDTPLLIDCEVSGIPFPSVV-WVKDGRVLMGEGYVIFDNGTLVLRRARPADSGRYTCTANNSRGKDHISSQPTIERPVAPRDIPLGDGSPTIA--RIADNATVLTGSRLTIDCV----ATGVPKPSIIWRK-DGRELIGAGSYMAPGNGSLLIQQVAASHHGVFTCTATNPVGEDSVSSNIRVRTTDKVEKSDMKPVDVRIGGQVTSLSGVD----ITLNCSARGLPPPDIEWMNGGKMI 370          
BLAST of hemicentin-1 isoform X2 vs. Ensembl Nematostella
Match: EDO32299 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SVH7])

HSP 1 Score: 59.3066 bits (142), Expect = 2.162e-9
Identity = 67/262 (25.57%), Postives = 109/262 (41.60%), Query Frame = 3
Query:  840 TIDLSCEVYANPAATIQWVIMQGLQIIDSSKFIAK---------IRPDMDGPLICEAKNYLGSTKETVQLNVL----RDPLIHIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMTDSSAVLISD----DAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKW-IDVE--TKDIVSDMETFQVI--NSESRHNGKYKCLAENEI 1559
            T+ + C     PA  + W  M    +    +F+ +         ++ D  G + C A+N  G    T +LNV+     +  I I  + RV +  N  I+C +   P P  + W + +    S+A L  D    D  +N IL   S TG Y C   N              EK+ F+TVT        +G   I+++ G K +++C     S G P P+  W ++ E   K   +D+   +++        +GKY+C A N I
Sbjct:    6 TLTIICPTRGLPAPDVTWE-MNNTNVRPGGRFVVQEGTLTIRNLVKQDQ-GNIRCVARNAAGQDTATSKLNVIDGDGEEARITIGQDLRVVEKMNVRIHCPIVGFPKP-VVTWTRNNQDVRSNARLFVDPNTNDLLINNILT--SDTGQYICSAVN---------PIGEDEKSSFITVTAT------IGTT-ITLLAGAKLVLQC----PSTGVPLPSTSWSLNGEPLEKGGRADLRGTELVIPAVAGVDSGKYQCTASNVI 242          
BLAST of hemicentin-1 isoform X2 vs. Ensembl Nematostella
Match: EDO29616 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T347])

HSP 1 Score: 60.4622 bits (145), Expect = 3.056e-9
Identity = 70/293 (23.89%), Postives = 110/293 (37.54%), Query Frame = 3
Query:  771 LNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQWV-------IMQGLQIIDSSKFIAKIRPDMDGPLICEAKNYL--GSTKETVQLNVLRDPLIHIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKP----KEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETKDIVSDMETFQVINSESR-HNGKYKCLAENEIGKSFSRETALVTVNEA 1607
            LN+K  P     + ++++   N  +  +C     PA  + WV       +  G     ++  + KI+    G   C+A N +  G+  + V + V   P I    N  V      ++ C V  NP P  + W K  +   +   + +       +      TG Y C   N     T  R       + ++ V YKP      P    N+ I  +K     + C +D    G P PT+ W       +V    +    NS +R   G Y C A NE+G   +  T  VTVN A
Sbjct:  112 LNVKVAPAFTSSLSSSLDVAENDDVTNTCSASGRPAPNVTWVNKTSGSPVAHGTG--SATLSLLKIQRHQAGLYQCQAINDVGRGAITQDVTIIVQYPPEITPIQNTTVRAGETITLTCAVAGNPTPS-VSWSKAGSGHSTGGNVFTKAGATKAV------TGQYVCTAVNTVTGNTQTRT-----ASTYVMVKYKPSIISSYPGQTVNETIDFLK-----LTCISD----GYPAPTVTW---SRDGLVVSRTSVYYSNSVTRSEAGTYTCTATNEMGSDAT--TVQVTVNCA 376          
BLAST of hemicentin-1 isoform X2 vs. Ensembl Medaka
Match: ENSORLT00000012598.2 (roundabout homolog 1 [Source:NCBI gene;Acc:101156600])

HSP 1 Score: 96.2857 bits (238), Expect = 1.653e-19
Identity = 147/660 (22.27%), Postives = 243/660 (36.82%), Query Frame = 3
Query:  738 DAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQWVI-------------MQGLQIIDSSKFIAKI------RPDMDGPLICEAKNYLG---STKETVQLNVLRDPLIHIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYK-LSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETK------DIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARW-RYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGI-----------ASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASN--------------DQGIKKVNFEVVFKSQPDPPYDLRVTDFEWDSVTINWKPGFNG-GLTQNYEILVISENFGTQNVPFYSDKNENTLTITGLYPSTPYEIRIYAVNSIGRS 2549
            D+ +  K + L    + P+I    P+ +     +   L+C+    P  T++W                  + +   S F  +I      +PD DG  +C A+NYLG   S   ++++ +LRD       +  V       + C+     P   I W K  +N+ D    +     KL      ++  G Y CV  N+     G+R+   +E T+    T+  +   ++      ++  E     C       G+P P ++W   ++       +++ D  T  +    S   G Y C+ EN +GKS    +A +TV+  P+  T  +N+ V +      ++++F CE  G P     W R G+E +      F  +  +  S   +SQ+ + LTI          T ++ S  G Y+C+A N  G            A  DY   I         +        L  ++V + CQ        + W K  + +VL     + S+ E                     L I   E  D   Y+C ASN              + G+ K +  V     P  P    VT+    SVT++WK   +   L  +Y I   S   G + V         T  +  L P   Y   I AVN+ G S
Sbjct:   53 DSNLGYKGSRLRQEDFPPRIVEH-PSDLIVSKGEPATLNCKAEGRPPPTVEWYKDGERVETDRDNPRSHRMLLPSGSLFFLRIIHGRRSKPD-DGSYVCVARNYLGQAISHNASLEVAILRDDFRQNPVDVMVAVGEPAVLECQPPRGHPEPTISWRKDGANLDDRDERITIRSGKLMITNTLKTDAGKYICVATNM----LGERESETAELTVLERPTFVKRPSSVV------VLTDESVEFHCVVH----GDPVPIVRWRRDDSDLPKGRFEVLVD-HTLIIRQVTSSDEGSYTCVVENMVGKS--EASASLTVHVPPAFATHPRNQVVSVG-----RTVTFQCEATGNPQPAIFWQREGSESLL-----FSYQPPQPFSRLSVSQMGS-LTI----------TDVQRSDAGFYSCQALNIAGSVITKALLEVSDAGSDYLPPIIKQGPSNQTV--------LVDSIVELGCQTAGTPPPTVHWKK--DGVVLSPADSRTSLTE------------------TGSLKIYHTELGDSGMYTCTASNPGGEASWTAFLQVQEFGVGKSDHPVENNMIPTAPSKPEVTNISQTSVTLSWKSSPDKEKLPTSYLIEAFSYTLGNRWVTLAEHVRAETFVLRNLKPGAVYLFMIRAVNTYGLS 644          

HSP 2 Score: 71.2478 bits (173), Expect = 6.745e-12
Identity = 110/485 (22.68%), Postives = 183/485 (37.73%), Query Frame = 3
Query:  903 QGLQIIDSSKFIAKIRPDMDGPLICEAKNYLGST--KETVQLNVLRDPLIHIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMTDS------SAVLISDDAKLNFILI-----DRSSTGMYACVTQNIQISTTGKRKPANSE-KTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKW------IDVETKDIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGL-KVNELTIKDLENDDFSNYSCVASNDQG--IKKVNFEV 2288
            QG + + +    +K  P+ D  L      Y GS   +E     ++  P     S+  V K    ++NC+    PPP  ++WYK     ++      S  ++     L F+ I      +   G Y CV +N      G+    N+  +   L   ++     ++      +  GE  +++C   R   G P PT+ W      +D   + I        + N+     GKY C+A N +G+  S ETA +TV E P+ + +  +  V        +S+ F C   G P+   RWR  +  +P    +  + DH  I    I Q+                    SS  G Y C  +N  G +     L +  PP    + R++ ++       V  +C+A       I W +                 E+ +  Y+P +      + ++  LTI D++  D   YSC A N  G  I K   EV
Sbjct:   34 QGSREVSTKSPQSKTEPNQDSNL-----GYKGSRLRQEDFPPRIVEHP-----SDLIVSKGEPATLNCKAEGRPPPT-VEWYKDGERVETDRDNPRSHRMLLPSGSLFFLRIIHGRRSKPDDGSYVCVARNY----LGQAISHNASLEVAILRDDFRQNPVDVM------VAVGEPAVLECQPPR---GHPEPTISWRKDGANLDDRDERITIRSGKLMITNTLKTDAGKYICVATNMLGERES-ETAELTVLERPTFVKRPSSVVVLTD-----ESVEFHCVVHGDPVPIVRWRRDDSDLPKGRFEVLV-DHTLI----IRQV-------------------TSSDEGSYTCVVENMVGKSEASASLTVHVPPAFATHPRNQVVSVG---RTVTFQCEATGNPQPAIFWQREGS--------------ESLLFSYQPPQPFSRLSVSQMGSLTITDVQRSDAGFYSCQALNIAGSVITKALLEV 447          
BLAST of hemicentin-1 isoform X2 vs. Ensembl Medaka
Match: robo1 (roundabout guidance receptor 1 [Source:NCBI gene;Acc:101157073])

HSP 1 Score: 92.4337 bits (228), Expect = 2.423e-18
Identity = 112/472 (23.73%), Postives = 178/472 (37.71%), Query Frame = 3
Query:  978 EAKNYLGSTKETVQLNVLRDPLIHIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMTDS------SAVLISDDAKLNFILI-----DRSSTGMYACVTQNIQISTTGKRKPANSE-KTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKW------IDVETKDIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVL-----PFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQG--IKKVNFEV--VFKSQPDP 2312
            E  N LG T   ++       ++   S+  V K    ++NC+    P P  ++WYK     ++      S  ++     L F+ I      +   G Y CV +N      G+    N+  +   L   ++     ++      +  GE  +++C   R   G P PT+ W      ID   + I        + N+     GKY C+  N +G+  S E A +TV E PS + +  ++ V +      QS+ F CE RG P+   RWR  + ++P    + + EDH               T+ +          L S+  G Y C A+N  G A     L +  PP      R++ +        V  +C+A       I W +     +L     P  S ++S+ +                    +LTI D E  D   YSC A N  G  I K   EV  V   +P P
Sbjct:   68 EEDNSLGYTGSRLRQEDTPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPT-VEWYKDGERVETDRDNPRSQRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNY----LGEAVSHNASLEVAILRDDFRQNPADVM------VAAGEPAVLECQPPR---GHPEPTISWKKDGVNIDDRDERITIRGGKLMITNARKSDAGKYVCVGTNMVGERDS-EVAELTVLERPSFVRRPGSQVVLVD-----QSVEFRCEARGDPVPTVRWRKDDGELPKGRYEIR-EDH---------------TLKIR--------RLTSADVGSYTCVAENMMGKAEASATLTVHVPPAFVVRPRNQVVGVG---RTVTFQCEATGNPQPAIFWQREGSQNLLFSYQPPQPSSRFSVSQ------------------TGDLTITDAERSDMGYYSCQALNIAGSVITKALLEVTDVVSDRPPP 474          

HSP 2 Score: 58.5362 bits (140), Expect = 5.212e-8
Identity = 103/455 (22.64%), Postives = 167/455 (36.70%), Query Frame = 3
Query:  360 RLTIHNISSDNSGYYVC--RVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMY---IVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFV--PNTVNAMINQTIDLSCEVYANPAATIQWV-----IMQGLQII--DSSKFIAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLIHI-DSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMT---------DSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKW----IDVETKDI-VSDMET--FQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHK 1631
            R+     S  + G YVC  R  L     H  SL V  ++       F  N +  +    E   ++C    G P P IS     +  D+  +R+ I       +   +  A            G  +C   N  G      + L +  +P    FV  P +   +++Q+++  CE   +P  T++W      + +G   I  D +  I ++     G   C A+N +G  + +  L V   P   +   N  V      +  C  T NP P  I W +  +            SS   +S    L     +RS  G Y+C   NI  S   K   A  E T  ++    P   Q   N+ + +       + C     + G P PT+ W    + V T D  V  ++T   Q+  ++    G Y C+A    G++  +  + V V E  S I  H+
Sbjct:  150 RIVHGRRSKPDEGSYVCVARNYLGEAVSHNASLEVAILRD-----DFRQNPADVMVAAGEPAVLECQPPRGHPEPTISWKKDGVNIDDRDERITIRGGKLMITNARKSDA------------GKYVCVGTNMVGERDSEVAELTVLERPS---FVRRPGSQVVLVDQSVEFRCEARGDPVPTVRWRKDDGELPKGRYEIREDHTLKIRRLTSADVGSYTCVAENMMGKAEASATLTVHVPPAFVVRPRNQVVGVGRTVTFQCEATGNPQPA-IFWQREGSQNLLFSYQPPQPSSRFSVSQTGDLTITDAERSDMGYYSCQALNIAGSVITK---ALLEVTDVVSDRPPPVIRQGPTNQTVPVDG--TVDLNC----MAAGNPTPTILWRKDGVLVSTHDSRVKQLDTGALQIRYAKLGDTGTYTCIASTPSGEASWK--SFVEVQEFGSPIQPHR 572          
BLAST of hemicentin-1 isoform X2 vs. Ensembl Medaka
Match: ENSORLT00000042592.1 (roundabout homolog 2 [Source:NCBI gene;Acc:101156842])

HSP 1 Score: 92.4337 bits (228), Expect = 2.438e-18
Identity = 134/585 (22.91%), Postives = 232/585 (39.66%), Query Frame = 3
Query:  942 KIRPDMDGPLICEAKNYLG---STKETVQLNVLRDPLIHIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMTDSSAVLISDD------AKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWI--DVETKDIVSDMETF--QVINSESRHNGKYKCLAENEIGKSFSRETALVTVN----EAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERIS-QIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHG--IASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMI-RCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQG---------IKKVNFEVVFK---------------SQPDPPYDLRVTDFEWDSVTINWKPGFNGGLTQNYEILVISENFGT------QNVPFYSDKNENTLTITGLYPSTPYEIRIYAVNSIG 2543
            + +PD DG  +C A+NY+G   S   ++++ +LRD       +  V      +  C+     P     W K     D + + +SDD       KL      +S +G++ CV  N+     G+R+   SEK   L+V  KP   Q   N+++ + +  +F  +C       G+P P L+W   DV+      + E F  ++  +     G + C AEN +GK  +  +A +T+     EAP  + + +++ V        ++ +F CE RG+P     W+         S+D    +     + R+S  +  +LTI          +S++ S  G Y C+A    G  +A     +           IR      +     V + RCQA       + W K   +++   +  ++S+ E                     L I++    D   Y+CVA++  G         ++ ++  V F                + P PP    VT+    S++++W+PG   G   +  ++   E FG       Q V  +    E   T+T L P T Y   I AVN+ G
Sbjct:  100 RSKPD-DGNYVCVARNYMGEAVSRNASLEVALLRDDFRQNPQDVVVAVGETATFECQPPRGHPEPVTFWRK-----DKTRLDLSDDRITVRGGKLTISNTKKSDSGIFVCVASNM----VGERE---SEKAQ-LSVFEKPSFVQRPVNQVVLVDESVEF--RCQVH----GDPPPVLRWKKEDVDIPRGRYEKEDFLLRIKKASVSDQGTFTCSAENRVGK--TEASAYLTIRARPVEAPQFVVRPRDQIVAQG-----RTATFPCETRGKPQPTVFWQR------EGSKDLLFPNQSAQGDSRVSVSVNGELTI----------SSVQRSDAGYYICQALTVAGSIMAKAQLEVADALKDRPPPIIRQGPSNQTQALGAVSVFRCQASGDPEPTVTWRKNGANLL--GKDPRFSVLEH------------------GSLQIQNSRLSDAGLYTCVATSSSGETSWSAYLDVRGLHCLVFFAFYNVISIYFVFHNSTTLPGPPSKPEVTNVTKSSISLSWEPGPEAGSPVSSYVI---EAFGQSVSNSWQTVADHVKTTE--FTVTDLRPKTVYLFIIRAVNAQG 616          

HSP 2 Score: 58.151 bits (139), Expect = 6.560e-8
Identity = 101/458 (22.05%), Postives = 175/458 (38.21%), Query Frame = 3
Query: 1041 LIHIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMTDS------SAVLISDDAKLNFILI-----DRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETK-DIVSDMETFQ-----VINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAA------SLNQNVVMIR-----CQAESISTMHIKWYK-TNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVN-ELTIKDLENDDFSNYSCVASNDQG--IKKVNFEV--VFKSQPDP 2312
            ++   S+  V K    ++NC+    P P  ++WYK     ++      S  ++     L F+ I      +   G Y CV +N      G+    N+   + L      + PQ   + ++++  GE    +C   R   G P P   W   +T+ D+  D  T +     + N++   +G + C+A N +G+  S E A ++V E PS + +  N+ V +      +S+ F C+  G P    RW+  +  IP     ++ ED                 + +   + S Q        G + C A+NR G      +L           IR R + A        +Q V   R     C+        + W +  ++ ++ P QS +    ++ V+            + VN ELTI  ++  D   Y C A    G  + K   EV    K +P P
Sbjct:   33 IVEHPSDLIVSKGEPATLNCKAEGRPIPS-VEWYKDGERVETDRDNPRSHRMLLPSGSLFFLRIVHGRRSKPDDGNYVCVARNYM----GEAVSRNASLEVALLRDDFRQNPQ---DVVVAV--GETATFECQPPR---GHPEPVTFWRKDKTRLDLSDDRITVRGGKLTISNTKKSDSGIFVCVASNMVGERES-EKAQLSVFEKPSFVQRPVNQVVLVD-----ESVEFRCQVHGDPPPVLRWKKEDVDIP--RGRYEKED---------------FLLRIKKASVSDQ--------GTFTCSAENRVGKTEASAYL----------TIRARPVEAPQFVVRPRDQIVAQGRTATFPCETRGKPQPTVFWQREGSKDLLFPNQSAQ---GDSRVS------------VSVNGELTISSVQRSDAGYYICQALTVAGSIMAKAQLEVADALKDRPPP 421          

HSP 3 Score: 56.225 bits (134), Expect = 2.294e-7
Identity = 65/321 (20.25%), Postives = 124/321 (38.63%), Query Frame = 3
Query:  705 NRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVN--AMINQTIDLSCEVYANPAATIQWV-----IMQGLQIIDSSKFIAKIRPDM---DGPLICEAKNYLGSTKETVQLNVLRDPLIHIDSNYR-----VPKNSNYSINCRVTSNPPPEFIKWYKLS---------NMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQIL--GNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKW-------IDVETKDIVSDMETFQVINSESRHNGKYKCLAENEIGKS 1568
            + G  +C A N  G      + L++  KP    FV   VN   +++++++  C+V+ +P   ++W      I +G    +   F+ +I+       G   C A+N +G T+ +  L +   P+       R     V +    +  C     P P    W +           +    S V +S + +L    + RS  G Y C    +  S   K +       + +    K + P I+  G    +   G   + +C    ++ G+P PT+ W       +  + +  V +  + Q+ NS     G Y C+A +  G++
Sbjct:  197 DSGIFVCVASNMVGERESEKAQLSVFEKPS---FVQRPVNQVVLVDESVEFRCQVHGDPPPVLRWKKEDVDIPRGR--YEKEDFLLRIKKASVSDQGTFTCSAENRVGKTEASAYLTIRARPVEAPQFVVRPRDQIVAQGRTATFPCETRGKPQPTVF-WQREGSKDLLFPNQSAQGDSRVSVSVNGELTISSVQRSDAGYYICQALTVAGSIMAKAQ-------LEVADALKDRPPPIIRQGPSNQTQALGAVSVFRC----QASGDPEPTVTWRKNGANLLGKDPRFSVLEHGSLQIQNSRLSDAGLYTCVATSSSGET 500          
BLAST of hemicentin-1 isoform X2 vs. Ensembl Medaka
Match: ENSORLT00000012625.2 (roundabout homolog 2 [Source:NCBI gene;Acc:101156842])

HSP 1 Score: 91.6633 bits (226), Expect = 3.571e-18
Identity = 135/581 (23.24%), Postives = 234/581 (40.28%), Query Frame = 3
Query:  942 KIRPDMDGPLICEAKNYLG---STKETVQLNVLRDPLIHIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMTDSSAVLISDD------AKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKW----IDVETK--DIVSDMETF--QVINSESRHNGKYKCLAENEIGKSFSRETALVTVN----EAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERIS-QIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHG--IASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMI-RCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQG-------------IKKVNFEVVFKSQ---PDPPYDLRVTDFEWDSVTINWKPGFNGGLTQNYEILVISENFGT------QNVPFYSDKNENTLTITGLYPSTPYEIRIYAVNSIG 2543
            + +PD DG  +C A+NY+G   S   ++++ +LRD       +  V      +  C+     P     W K     D + + +SDD       KL      +S +G++ CV  N+     G+R+   SEK   L+V  KP   Q   N+++ + +  +F  +C       G+P P L+W    +D+     +I  + E F  ++  +     G + C AEN +GK  +  +A +T+     EAP  + + +++ V        ++ +F CE RG+P     W+         S+D    +     + R+S  +  +LTI          +S++ S  G Y C+A    G  +A     +           IR      +     V + RCQA       + W K   +++   +  ++S+ E                     L I++    D   Y+CVA++  G             +  ++F  VF +    P PP    VT+    S++++W+PG   G   +  ++   E FG       Q V  +    E   T+T L P T Y   I AVN+ G
Sbjct:   98 RSKPD-DGNYVCVARNYMGEAVSRNASLEVALLRDDFRQNPQDVVVAVGETATFECQPPRGHPEPVTFWRK-----DKTRLDLSDDRITVRGGKLTISNTKKSDSGIFVCVASNM----VGERE---SEKAQ-LSVFEKPSFVQRPVNQVVLVDESVEF--RCQVH----GDPPPVLRWKKEDVDIPRGRYEIRYEKEDFLLRIKKASVSDQGTFTCSAENRVGK--TEASAYLTIRARPVEAPQFVVRPRDQIVAQG-----RTATFPCETRGKPQPTVFWQR------EGSKDLLFPNQSAQGDSRVSVSVNGELTI----------SSVQRSDAGYYICQALTVAGSIMAKAQLEVADALKDRPPPIIRQGPSNQTQALGAVSVFRCQASGDPEPTVTWRKNGANLL--GKDPRFSVLEH------------------GSLQIQNSRLSDAGLYTCVATSSSGETSWSAYLDVRDSMDPIDF--VFHNSTTLPGPPSKPEVTNVTKSSISLSWEPGPEAGSPVSSYVI---EAFGQSVSNSWQTVADHVKTTE--FTVTDLRPKTVYLFIIRAVNAQG 608          

HSP 2 Score: 58.5362 bits (140), Expect = 4.543e-8
Identity = 100/458 (21.83%), Postives = 174/458 (37.99%), Query Frame = 3
Query: 1041 LIHIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMTDS------SAVLISDDAKLNFILI-----DRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETK-DIVSDMETFQ-----VINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAA------SLNQNVVMIR-----CQAESISTMHIKWYK-TNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVN-ELTIKDLENDDFSNYSCVASNDQG--IKKVNFEV--VFKSQPDP 2312
            ++   S+  V K    ++NC+    P P  ++WYK     ++      S  ++     L F+ I      +   G Y CV +N      G+    N+   + L      + PQ   + ++++  GE    +C   R   G P P   W   +T+ D+  D  T +     + N++   +G + C+A N +G+  S E A ++V E PS + +  N+ V +      +S+ F C+  G P    RW+  +  IP    + + E                  + +   + S Q        G + C A+NR G      +L           IR R + A        +Q V   R     C+        + W +  ++ ++ P QS +    ++ V+            + VN ELTI  ++  D   Y C A    G  + K   EV    K +P P
Sbjct:   31 IVEHPSDLIVSKGEPATLNCKAEGRPIPS-VEWYKDGERVETDRDNPRSHRMLLPSGSLFFLRIVHGRRSKPDDGNYVCVARNYM----GEAVSRNASLEVALLRDDFRQNPQ---DVVVAV--GETATFECQPPR---GHPEPVTFWRKDKTRLDLSDDRITVRGGKLTISNTKKSDSGIFVCVASNMVGERES-EKAQLSVFEKPSFVQRPVNQVVLVD-----ESVEFRCQVHGDPPPVLRWKKEDVDIPRGRYEIRYEKE-------------DFLLRIKKASVSDQ--------GTFTCSAENRVGKTEASAYL----------TIRARPVEAPQFVVRPRDQIVAQGRTATFPCETRGKPQPTVFWQREGSKDLLFPNQSAQ---GDSRVS------------VSVNGELTISSVQRSDAGYYICQALTVAGSIMAKAQLEVADALKDRPPP 423          

HSP 3 Score: 55.8398 bits (133), Expect = 3.632e-7
Identity = 66/323 (20.43%), Postives = 125/323 (38.70%), Query Frame = 3
Query:  705 NRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVN--AMINQTIDLSCEVYANPAATIQWV-----IMQGLQII--DSSKFIAKIRPDM---DGPLICEAKNYLGSTKETVQLNVLRDPLIHIDSNYR-----VPKNSNYSINCRVTSNPPPEFIKWYKLS---------NMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQIL--GNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKW-------IDVETKDIVSDMETFQVINSESRHNGKYKCLAENEIGKS 1568
            + G  +C A N  G      + L++  KP    FV   VN   +++++++  C+V+ +P   ++W      I +G   I  +   F+ +I+       G   C A+N +G T+ +  L +   P+       R     V +    +  C     P P    W +           +    S V +S + +L    + RS  G Y C    +  S   K +       + +    K + P I+  G    +   G   + +C    ++ G+P PT+ W       +  + +  V +  + Q+ NS     G Y C+A +  G++
Sbjct:  195 DSGIFVCVASNMVGERESEKAQLSVFEKPS---FVQRPVNQVVLVDESVEFRCQVHGDPPPVLRWKKEDVDIPRGRYEIRYEKEDFLLRIKKASVSDQGTFTCSAENRVGKTEASAYLTIRARPVEAPQFVVRPRDQIVAQGRTATFPCETRGKPQPTVF-WQREGSKDLLFPNQSAQGDSRVSVSVNGELTISSVQRSDAGYYICQALTVAGSIMAKAQ-------LEVADALKDRPPPIIRQGPSNQTQALGAVSVFRC----QASGDPEPTVTWRKNGANLLGKDPRFSVLEHGSLQIQNSRLSDAGLYTCVATSSSGET 502          
BLAST of hemicentin-1 isoform X2 vs. Ensembl Medaka
Match: igsf9bb (protein turtle homolog B-like [Source:NCBI gene;Acc:101174449])

HSP 1 Score: 91.2781 bits (225), Expect = 4.550e-18
Identity = 119/522 (22.80%), Postives = 211/522 (40.42%), Query Frame = 3
Query:  159 PNNATVNSKENLTLECVVDQQNIVYSWEYKPFHIKSNIMSKVLGFCY---FEKPCVVPDSESQITISHESRLTIHNISSDNSGYYVCRVTLLTT---SEHIISLAVVTVKTPPKSISFHFNKSINVF---QEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFV---PNTVNAMINQTIDLSCEVYANPA-ATIQWVIMQ-------GLQ-----IIDSSKFIAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDP--LIHIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYK---LSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDV----ETKDIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIIT 1622
            P   T  + E++ L C     N+ +    +P+ ++       + F     F  P V P+   + ++  +S L I N+ SD+ G+Y C+V +L     + H  S   +TV  PP      F  +   +   +E   I + C   G    PK S+  +++ ++    ++SS     +   ++ +S+ R    ++RG  MC A +  G     T LL      +  PF+   P  +   I+Q    +C+  A P   T  W   Q        L+     +ID S  IA+++P+  G   C   N LG          ++ P  ++++ S   V       I C V +NPP   +KW K      +         DD  +    +   S G Y+C+  N  + + G   PA       L +   PK   + G +    + G + +I C  +    G+PFP + W  V     +K  V    + Q+ +     +G+++C+A N +  S +  T +  +  +P   T
Sbjct:   30 PQFVTARAGESVILGC-----NVPHPLNGQPYVVEWFKYGMPIPFFINFRFYPPHVDPEYAGRASLHGKSSLRIDNVRSDDHGWYECKVLMLQQQYDTFHNGSWVHLTVNAPPT-----FTDTPPQYVEAKEGGSITLTCMAFGN---PKPSVSWLREGSL----MVSSAKYKLSDGSLTVLSITR----QDRGAYMCRAFSLQGEAIHTTRLLV-----QGPPFIISPPENITVNISQDAFFTCQAEAYPRNLTYTWFWEQDNVFFKNDLKRRVSILIDGSLIIAQVKPEDAGKYTCVPSNSLGQPPSASAYLTVQYPARVVNMPSVIYVAIGLPGFIRCPVDANPPVTLVKWKKDGLFLRIDKFPGWSQMDDGSIRVAEVTEDSLGTYSCMPYN-ALGSMGWSPPAP------LVLKDPPKFSVVPGGEYRQEV-GRELVIPCEAE----GDPFPNITWRKVGKPSRSKHNVLPRGSLQLKSLTKEDHGEWECVATN-VATSITASTHVQVIGTSPHAPT 512          

HSP 2 Score: 53.9138 bits (128), Expect = 1.236e-6
Identity = 39/140 (27.86%), Postives = 61/140 (43.57%), Query Frame = 3
Query: 2181 LKVNELTIKDLENDDFSNYSCVASN-DQGIKKVNFEVVFKSQPDPPYDLR-VTDFEWDSVTINWKPGFNGGLTQNYEILVISENFGTQN-----VPFYSDKNENTLTITGLYPSTPYEIRIYAVNSIGRSLNSETVKCVT 2579
            L    L +K L  +D   + CVA+N    I       V  + P  P ++  V    W +V+  W+ G++GG  Q + + +  E  G  +     VP      +N   ++GL P T Y+  + A N +G S  SE V   T
Sbjct:  466 LPRGSLQLKSLTKEDHGEWECVATNVATSITASTHVQVIGTSPHAPTNVHAVASSTWANVS--WEAGYSGGFQQTFSVWIKRERLGPHDWLSTAVP----GGQNWKLVSGLEPETTYQFSVLAQNKLGTSPFSEVVTVNT 599          
BLAST of hemicentin-1 isoform X2 vs. Planmine SMEST
Match: SMESG000003113.1 (SMESG000003113.1)

HSP 1 Score: 2290 bits (5933), Expect = 0.000e+0
Identity = 1177/1183 (99.49%), Postives = 1178/1183 (99.58%), Query Frame = 3
Query:   75 MEYFKTFILITLFGNLMDRSYTLNFLITPNNATVNSKENLTLECVVDQQNIVYSWEYKPFHIKSNIMSKVLGFCYFEKPCVVPDSESQITISHESRLTIHNISSDNSGYYVCRVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQWVIMQGLQIIDSSKFIAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLIHIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETKDIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQPDPPYDLRVTDFEWDSVTINWKPGFNGGLTQNYEILVISENFGTQNVPFYSDKNENTLTITGLYPSTPYEIRIYAVNSIGRSLNSETVKCVTKRIRKFDKIQAKILSDQPKIAVIIQEPDVCANLEYTRDNGHQWFSILHCIRFNASHINETVLLDIIRVASILDLQSGLIHLRLTFCLISRPSVCSPHRILENPKNENLATQXXXXXXXXXXXXXXXXXXXXXXXXXXRFRYHCVSCNQYFCHNYCCFPRFYGATNKPIRHYNNNRIPYDGKPLSCSRVSCQDILDENDINTGSTSNYCSSLIGSSTPHIMNKKCPQNSSNDVYSSFICPHHEFRSHIPVLYEGSSFLQPTVLSWSQKIXXXXXXXXXXXXXXXXXXXXXRNLPQNSVHYRKKQDGISNSNVVNHKSYAQTEGIIKLPDVTSEAIHFVKPDYNEKLMEA 3623
            MEYFKTFILITLFGNLMDRSYTLNFLITPNNATVNSKENLTLECVVDQQNIVYSWEYKPFHIKSN MSKVLGFCYFEKPCVVPDSESQITISHESRLTIHNISSDNSGYYVCRVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVY+NPAATIQWVIMQGLQIIDSSKFIAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLIHIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETKDIVSD ETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYS TFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLIIKYPPIIKYNIRHRKIAASLNQNV MIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQPDPPYDLRVTDFEWDSVTINWKPGFNGGLTQNYEILVISENFGTQNVPFYSDKNENTLTITGLYPSTPYEIRIYAVNSIGRSLNSETVKCVTKRIRKFDKIQAKILSDQPKIAVIIQEPDVCANLEYTRDNGHQWFSILHCIRFNASHINETVLLDIIRVASILDLQSGLIHLRLTFCLISRPSVCSPHRILENPKNENLATQTVILVITVICFLLIIILIALFIVCCIRFRYHCVSCNQYFCHNYCCFPRFYGATNKPIRHYNNNRIPYDGKPLSCSRVSCQDILDENDINTGSTSNYCSSLIGSSTPHIMNKKCPQNSSNDVYSSFICPHHEFRSHIPVLYEGSSFLQPTVLSWSQKISPPSCEASSSHSHSSSTGSSSRNLPQN VHYRKKQDGISNSNVVNHKSYAQTEGIIKLPDVTSEAIHFVKPDYNEKLMEA
Sbjct:    1 MEYFKTFILITLFGNLMDRSYTLNFLITPNNATVNSKENLTLECVVDQQNIVYSWEYKPFHIKSNSMSKVLGFCYFEKPCVVPDSESQITISHESRLTIHNISSDNSGYYVCRVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYSNPAATIQWVIMQGLQIIDSSKFIAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLIHIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETKDIVSDTETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSPTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLIIKYPPIIKYNIRHRKIAASLNQNVAMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQPDPPYDLRVTDFEWDSVTINWKPGFNGGLTQNYEILVISENFGTQNVPFYSDKNENTLTITGLYPSTPYEIRIYAVNSIGRSLNSETVKCVTKRIRKFDKIQAKILSDQPKIAVIIQEPDVCANLEYTRDNGHQWFSILHCIRFNASHINETVLLDIIRVASILDLQSGLIHLRLTFCLISRPSVCSPHRILENPKNENLATQTVILVITVICFLLIIILIALFIVCCIRFRYHCVSCNQYFCHNYCCFPRFYGATNKPIRHYNNNRIPYDGKPLSCSRVSCQDILDENDINTGSTSNYCSSLIGSSTPHIMNKKCPQNSSNDVYSSFICPHHEFRSHIPVLYEGSSFLQPTVLSWSQKISPPSCEASSSHSHSSSTGSSSRNLPQNPVHYRKKQDGISNSNVVNHKSYAQTEGIIKLPDVTSEAIHFVKPDYNEKLMEA 1183          
BLAST of hemicentin-1 isoform X2 vs. Planmine SMEST
Match: SMESG000003113.1 (SMESG000003113.1)

HSP 1 Score: 2256.48 bits (5846), Expect = 0.000e+0
Identity = 1161/1167 (99.49%), Postives = 1162/1167 (99.57%), Query Frame = 3
Query:  123 MDRSYTLNFLITPNNATVNSKENLTLECVVDQQNIVYSWEYKPFHIKSNIMSKVLGFCYFEKPCVVPDSESQITISHESRLTIHNISSDNSGYYVCRVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQWVIMQGLQIIDSSKFIAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLIHIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETKDIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXNIRHRKIAASLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQPDPPYDLRVTDFEWDSVTINWKPGFNGGLTQNYEILVISENFGTQNVPFYSDKNENTLTITGLYPSTPYEIRIYAVNSIGRSLNSETVKCVTKRIRKFDKIQAKILSDQPKIAVIIQEPDVCANLEYTRDNGHQWFSILHCIRFNASHINETVLLDIIRVASILDLQSGLIHLRLTFCLISRPSVCSPHRILENPKNENLATQXXXXXXXXXXXXXXXXXXXXXXXXXXRFRYHCVSCNQYFCHNYCCFPRFYGATNKPIRHYNNNRIPYDGKPLSCSRVSCQDILDENDINTGSTSNYCSSLIGSSTPHIMNKKCPQNSSNDVYSSFICPHHEFRSHIPVLYEGSSFLQPTVLSWSQKIXXXXXXXXXXXXXXXXXXXXXRNLPQNSVHYRKKQDGISNSNVVNHKSYAQTEGIIKLPDVTSEAIHFVKPDYNEKLMEA 3623
            MDRSYTLNFLITPNNATVNSKENLTLECVVDQQNIVYSWEYKPFHIKSN MSKVLGFCYFEKPCVVPDSESQITISHESRLTIHNISSDNSGYYVCRVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVY+NPAATIQWVIMQGLQIIDSSKFIAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLIHIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETKDIVSD ETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYS TFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLIIKYPPIIKYNIRHRKIAASLNQNV MIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQPDPPYDLRVTDFEWDSVTINWKPGFNGGLTQNYEILVISENFGTQNVPFYSDKNENTLTITGLYPSTPYEIRIYAVNSIGRSLNSETVKCVTKRIRKFDKIQAKILSDQPKIAVIIQEPDVCANLEYTRDNGHQWFSILHCIRFNASHINETVLLDIIRVASILDLQSGLIHLRLTFCLISRPSVCSPHRILENPKNENLATQTVILVITVICFLLIIILIALFIVCCIRFRYHCVSCNQYFCHNYCCFPRFYGATNKPIRHYNNNRIPYDGKPLSCSRVSCQDILDENDINTGSTSNYCSSLIGSSTPHIMNKKCPQNSSNDVYSSFICPHHEFRSHIPVLYEGSSFLQPTVLSWSQKISPPSCEASSSHSHSSSTGSSSRNLPQN VHYRKKQDGISNSNVVNHKSYAQTEGIIKLPDVTSEAIHFVKPDYNEKLMEA
Sbjct:    1 MDRSYTLNFLITPNNATVNSKENLTLECVVDQQNIVYSWEYKPFHIKSNSMSKVLGFCYFEKPCVVPDSESQITISHESRLTIHNISSDNSGYYVCRVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYSNPAATIQWVIMQGLQIIDSSKFIAKIRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLIHIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETKDIVSDTETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSPTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLIIKYPPIIKYNIRHRKIAASLNQNVAMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQPDPPYDLRVTDFEWDSVTINWKPGFNGGLTQNYEILVISENFGTQNVPFYSDKNENTLTITGLYPSTPYEIRIYAVNSIGRSLNSETVKCVTKRIRKFDKIQAKILSDQPKIAVIIQEPDVCANLEYTRDNGHQWFSILHCIRFNASHINETVLLDIIRVASILDLQSGLIHLRLTFCLISRPSVCSPHRILENPKNENLATQTVILVITVICFLLIIILIALFIVCCIRFRYHCVSCNQYFCHNYCCFPRFYGATNKPIRHYNNNRIPYDGKPLSCSRVSCQDILDENDINTGSTSNYCSSLIGSSTPHIMNKKCPQNSSNDVYSSFICPHHEFRSHIPVLYEGSSFLQPTVLSWSQKISPPSCEASSSHSHSSSTGSSSRNLPQNPVHYRKKQDGISNSNVVNHKSYAQTEGIIKLPDVTSEAIHFVKPDYNEKLMEA 1167          
BLAST of hemicentin-1 isoform X2 vs. Planmine SMEST
Match: SMESG000015102.1 (SMESG000015102.1)

HSP 1 Score: 185.652 bits (470), Expect = 2.431e-47
Identity = 209/861 (24.27%), Postives = 359/861 (41.70%), Query Frame = 3
Query:  363 LTIHNISSDNSGYYVCRVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKD---NAIKRLKIISSNFPNSTI--AEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQWVIMQGLQIIDSSKFIAK-IRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLIHIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQIS--TTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETKDIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSD----LPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIE-DHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXN--IRHRKIAA-SLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYE-PERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQPDPPYDLRVTDFEWDSVTINWKPGFNGGLTQNYEILVISENFGTQNVPFYSDKNENTLTITGLYPSTPYEIRIYAVNSIGRSLNSETVKCVTKRIRKFDKIQAKILSDQPKIAVI-----IQEPDVC---ANLEYTRDNGHQWFSILHCIRFNASHINETVLLDIIRVASILDLQSGLIHLRLTFCLISRPSVCSPH 2870
            +TI N +S NSG Y C    L+  ++      V++ TPP     + N  +    E     ++C+  GG PPP +  ++ KD   N I + + I +   +STI    ++  + W P   E+   + C A N    ++ N   +++ Y P I  F   T   +    I++ C+  +  AAT +W+  +G    D S  I K +R    G   C  KN  G++  ++ + VL +P I I     + +    ++ C V   P    + W     +  +   L+ +    N   I+  +   Y    +++Q S  TT KRK       + + V Y P++P I       +M G    + C  ++K PG P P   W    T    +      +   E  H+G Y C   N +G S     AL  V   P +      +    S+SD    + QS +  C F+ QP     W   +E +  +S   +   ++++ +N  I+ I            +  + ++  + N +Y C A+NR+G    DYF+ + Y P I  N  I+  KI+  S ++  V + C   +   + I W +   +    F  K   I E +  KY   ER+L     K + L IK+ ++DD+  Y C A+N +G    +F +   + PDP ++L +    + S+T+ W  GF+GG  Q      +S     Q  P       + +T+  L P   Y + I + N IG S    +++  T  +   D+ Q   ++   K+        +     C    N++  +D    W  +  CI+ +            IR+ +  D        R+  CLI+ P VCS +
Sbjct:  150 ITIRNANSKNSGLYKC--VHLSYPKYERHHYDVSILTPPGKPQIYTNAGLT---ENVTAHLKCTSEGGYPPPTLK-WVWKDFSNNLISKERRIKTTITSSTIKYGLITLSTTWLPKSNEDGDSLNCVAENSLATVSSNWVYISVNYAPIIYEFPQKTYYGLEGDEINIECKATSKSAATYKWISDKGTVYSDGSYLIIKPLRTSDKGSYSCVVKNSYGTSSASLDIKVLSEPKISIPEAVVINEWETLNLTCDVI--PKTSNVTWLLKDKVLSNLKNLVHNVVGHNSFEIECRA---YVFTKRHLQYSIITTSKRK-------VKVIVHYIPQKPTISAIPAGKLMTGSSVTLTCIPNKKKPGLPNPDYSWKKRHTDWYTNGTNFLSISTLEFEHSGIYCCKLFNSLGSSDESCYAL-NVEGKPVM-----KKSALSSFSDVSARIGQSFNIVCLFQAQPHPIINWYNNDELVENNSNSTRFNMENKSANNSLITSIL-----------YVNEPNV--NINEKYRCTAENRYGTIFKDYFVKVIYSPRILSNVFIKAAKISGFSSDKQSVNLNCTVTASPLIRILWIRKLHN----FNEK---IIENDTDKYLIKERNLLGIIGKESLLFIKNFQSDDYGAYICYANNSEGYIANDFIIRKFAIPDPAHNLVIESRTYCSLTLKWNAGFDGGYQQ---FFTVSYTTTMQKHPLQVRTIHSNITLINLLPEVLYIVNISSSNQIGTSTLVRSIETRTAEL-PIDRDQIFFVNKDEKVIEFSLPLNMSSSIFCLTIENMDTQKDLVGDWALVGKCIKNSIRK---------IRIENPND------RFRVKICLINLPDVCSTY 947          
BLAST of hemicentin-1 isoform X2 vs. Planmine SMEST
Match: SMESG000015102.1 (SMESG000015102.1)

HSP 1 Score: 185.267 bits (469), Expect = 2.702e-47
Identity = 208/861 (24.16%), Postives = 360/861 (41.81%), Query Frame = 3
Query:  363 LTIHNISSDNSGYYVCRVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKD---NAIKRLKIISSNFPNSTI--AEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQWVIMQGLQIIDSSKFIAK-IRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLIHIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQIS--TTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETKDIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSD----LPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIE-DHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXN--IRHRKIAA-SLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYE-PERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQPDPPYDLRVTDFEWDSVTINWKPGFNGGLTQNYEILVISENFGTQNVPFYSDKNENTLTITGLYPSTPYEIRIYAVNSIGRSLNSETVKCVTKRIRKFDKIQAKILSDQPKIAVIIQEPDVCA--------NLEYTRDNGHQWFSILHCIRFNASHINETVLLDIIRVASILDLQSGLIHLRLTFCLISRPSVCSPH 2870
            +TI N +S NSG Y C    L+  ++      V++ TPP     + N  +    E     ++C+  GG PPP +  ++ KD   N I + + I +   +STI    ++  + W P   E+   + C A N    ++ N   +++ Y P I  F   T   +    I++ C+  +  AAT +W+  +G    D S  I K +R    G   C  KN  G++  ++ + VL +P I I     + +    ++ C V   P    + W     +  +   L+ +    N   I+  +   Y    +++Q S  TT KRK       + + V Y P++P I       +M G    + C  ++K PG P P   W    T    +      +   E  H+G Y C   N +G S     AL  V   P +      +    S+SD    + QS +  C F+ QP     W   +E +  +S   +   ++++ +N  I+ I            +  + ++  + N +Y C A+NR+G    DYF+ + Y P I  N  I+  KI+  S ++  V + C   +   + I W +   +    F  K   I E +  KY   ER+L     K + L IK+ ++DD+  Y C A+N +G    +F +   + PDP ++L +    + S+T+ W  GF+GG  Q      +S     Q  P       + +T+  L P   Y + I + N IG S    +++  T  +   D+ Q   ++   K+       ++ +        N++  +D    W  +  CI+ +            IR+ +  D        R+  CLI+ P VCS +
Sbjct:  101 ITIRNANSKNSGLYKC--VHLSYPKYERHHYDVSILTPPGKPQIYTNAGLT---ENVTAHLKCTSEGGYPPPTLK-WVWKDFSNNLISKERRIKTTITSSTIKYGLITLSTTWLPKSNEDGDSLNCVAENSLATVSSNWVYISVNYAPIIYEFPQKTYYGLEGDEINIECKATSKSAATYKWISDKGTVYSDGSYLIIKPLRTSDKGSYSCVVKNSYGTSSASLDIKVLSEPKISIPEAVVINEWETLNLTCDVI--PKTSNVTWLLKDKVLSNLKNLVHNVVGHNSFEIECRA---YVFTKRHLQYSIITTSKRK-------VKVIVHYIPQKPTISAIPAGKLMTGSSVTLTCIPNKKKPGLPNPDYSWKKRHTDWYTNGTNFLSISTLEFEHSGIYCCKLFNSLGSSDESCYAL-NVEGKPVM-----KKSALSSFSDVSARIGQSFNIVCLFQAQPHPIINWYNNDELVENNSNSTRFNMENKSANNSLITSIL-----------YVNEPNV--NINEKYRCTAENRYGTIFKDYFVKVIYSPRILSNVFIKAAKISGFSSDKQSVNLNCTVTASPLIRILWIRKLHN----FNEK---IIENDTDKYLIKERNLLGIIGKESLLFIKNFQSDDYGAYICYANNSEGYIANDFIIRKFAIPDPAHNLVIESRTYCSLTLKWNAGFDGGYQQ---FFTVSYTTTMQKHPLQVRTIHSNITLINLLPEVLYIVNISSSNQIGTSTLVRSIETRTAEL-PIDRDQIFFVNKDEKVIEFSLPLNMSSSIFCLTIENMDTQKDLVGDWALVGKCIKNSIRK---------IRIENPND------RFRVKICLINLPDVCSTY 898          
BLAST of hemicentin-1 isoform X2 vs. Planmine SMEST
Match: SMESG000015102.1 (SMESG000015102.1)

HSP 1 Score: 184.882 bits (468), Expect = 4.064e-47
Identity = 208/861 (24.16%), Postives = 360/861 (41.81%), Query Frame = 3
Query:  363 LTIHNISSDNSGYYVCRVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQEQEFIAIQCSVSGGIPPPKISMYIVKD---NAIKRLKIISSNFPNSTI--AEVSAISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVNAMINQTIDLSCEVYANPAATIQWVIMQGLQIIDSSKFIAK-IRPDMDGPLICEAKNYLGSTKETVQLNVLRDPLIHIDSNYRVPKNSNYSINCRVTSNPPPEFIKWYKLSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQIS--TTGKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSPGEPFPTLKWIDVETKDIVSDMETFQVINSESRHNGKYKCLAENEIGKSFSRETALVTVNEAPSIITQHKNRKVFLSYSD----LPQSISFTCEFRGQPLYEARWRYGNEKIPPSSQDFKIE-DHRNISNERISQIFTKLTIDLYSRTFSQQTSLRSSPNGRYNCEADNRHGIASLDYFLXXXXXXXXXXN--IRHRKIAA-SLNQNVVMIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYE-PERDLFTPGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQPDPPYDLRVTDFEWDSVTINWKPGFNGGLTQNYEILVISENFGTQNVPFYSDKNENTLTITGLYPSTPYEIRIYAVNSIGRSLNSETVKCVTKRIRKFDKIQAKILSDQPKIAVIIQEPDVCA--------NLEYTRDNGHQWFSILHCIRFNASHINETVLLDIIRVASILDLQSGLIHLRLTFCLISRPSVCSPH 2870
            +TI N +S NSG Y C    L+  ++      V++ TPP     + N  +    E     ++C+  GG PPP +  ++ KD   N I + + I +   +STI    ++  + W P   E+   + C A N    ++ N   +++ Y P I  F   T   +    I++ C+  +  AAT +W+  +G    D S  I K +R    G   C  KN  G++  ++ + VL +P I I     + +    ++ C V   P    + W     +  +   L+ +    N   I+  +   Y    +++Q S  TT KRK       + + V Y P++P I       +M G    + C  ++K PG P P   W    T    +      +   E  H+G Y C   N +G S     AL  V   P +      +    S+SD    + QS +  C F+ QP     W   +E +  +S   +   ++++ +N  I+ I            +  + ++  + N +Y C A+NR+G    DYF+ + Y P I  N  I+  KI+  S ++  V + C   +   + I W +   +    F  K   I E +  KY   ER+L     K + L IK+ ++DD+  Y C A+N +G    +F +   + PDP ++L +    + S+T+ W  GF+GG  Q      +S     Q  P       + +T+  L P   Y + I + N IG S    +++  T  +   D+ Q   ++   K+       ++ +        N++  +D    W  +  CI+ +            IR+ +  D        R+  CLI+ P VCS +
Sbjct:  101 ITIRNANSKNSGLYKC--VHLSYPKYERHHYDVSILTPPGKPQIYTNAGLT---ENVTAHLKCTSEGGYPPPTLK-WVWKDFSNNLISKERRIKTTITSSTIKYGLITLSTTWLPKSNEDGDSLNCVAENSLATVSSNWVYISVNYAPIIYEFPQKTYYGLEGDEINIECKATSKSAATYKWISDKGTVYSDGSYLIIKPLRTSDKGSYSCVVKNSYGTSSASLDIKVLSEPKISIPEAVVINEWETLNLTCDVI--PKTSNVTWLLKDKVLSNLKNLVHNVVGHNSFEIECRA---YVFTKRHLQYSIITTSKRK-------VKVIVHYIPQKPTISAIPAGKLMTGSSVTLTCIPNKKKPGLPNPDYSWKKRHTDWYTNGTNFLSISTLEFEHSGIYCCKLFNSLGSSDESCYAL-NVEGKPVM-----KKSALSSFSDVSARIGQSFNIVCLFQAQPHPIINWYNNDELVENNSNSTRFNMENKSANNSLITSIL-----------YVNEPNV--NINEKYRCTAENRYGTIFKDYFVKVIYSPRILSNVFIKAAKISGFSSDKQSVNLNCTVTASPLIRILWIRKLHN----FNEK---IIENDTDKYLIKERNLLGIIGKESLLFIKNFQSDDYGAYICYANNSEGYIANDFIIRKFAIPDPAHNLVIESRTYCSLTLKWNAGFDGGYQQ---FFTVSYTTTMQKHPLQVRTIHSNITLINLLPEVLYIVNISSSNQIGTSTLVRSIETRTAEL-PIDRDQIFFVNKDEKVIEFSLPLNMSSSIFCLTIENMDTQKDLVGDWALVGKCIKNSIRK---------IRIENPND------RFRVKICLINLPDVCSTY 898          
The following BLAST results are available for this feature:
BLAST of hemicentin-1 isoform X2 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
MXRA51.764e-2222.70matrix remodeling associated 5 [Source:HGNC Symbol... [more]
ROBO22.084e-2223.17roundabout guidance receptor 2 [Source:HGNC Symbol... [more]
ROBO23.069e-2223.17roundabout guidance receptor 2 [Source:HGNC Symbol... [more]
ROBO23.170e-2223.17roundabout guidance receptor 2 [Source:HGNC Symbol... [more]
ROBO15.265e-2223.57roundabout guidance receptor 1 [Source:HGNC Symbol... [more]
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BLAST of hemicentin-1 isoform X2 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
igcm-18.007e-3023.30ImmunoGlobulin-like Cell adhesion Molecule family ... [more]
him-44.599e-2222.24High Incidence of Males (Increased X chromosome lo... [more]
him-45.491e-2222.24High Incidence of Males (Increased X chromosome lo... [more]
him-45.534e-2222.24High Incidence of Males (Increased X chromosome lo... [more]
syg-21.676e-1325.23Synaptogenesis protein syg-2 [Source:UniProtKB/Sw... [more]
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BLAST of hemicentin-1 isoform X2 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ed7.804e-3823.60gene:FBgn0000547 transcript:FBtr0334265[more]
ed7.804e-3823.60gene:FBgn0000547 transcript:FBtr0077465[more]
ed8.493e-3823.60gene:FBgn0000547 transcript:FBtr0334266[more]
fred1.026e-3623.14gene:FBgn0051774 transcript:FBtr0308126[more]
fred2.295e-3623.14gene:FBgn0051774 transcript:FBtr0308125[more]
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BLAST of hemicentin-1 isoform X2 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
hmcn11.471e-2322.82hemicentin 1 [Source:ZFIN;Acc:ZDB-GENE-041014-322][more]
hmcn11.561e-2322.82hemicentin 1 [Source:ZFIN;Acc:ZDB-GENE-041014-322][more]
hmcn21.402e-2024.12hemicentin 2 [Source:ZFIN;Acc:ZDB-GENE-030131-9765... [more]
robo24.444e-1824.03roundabout, axon guidance receptor, homolog 2 (Dro... [more]
robo24.444e-1824.03roundabout, axon guidance receptor, homolog 2 (Dro... [more]
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BLAST of hemicentin-1 isoform X2 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
lrrc8c5.254e-2422.80leucine rich repeat containing 8 VRAC subunit C [S... [more]
lrrc8c7.497e-2423.16leucine rich repeat containing 8 VRAC subunit C [S... [more]
lrrc8c9.069e-2422.68leucine rich repeat containing 8 VRAC subunit C [S... [more]
lrrc8c1.072e-2322.68leucine rich repeat containing 8 VRAC subunit C [S... [more]
lrrc8c1.312e-2322.68leucine rich repeat containing 8 VRAC subunit C [S... [more]
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BLAST of hemicentin-1 isoform X2 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Robo19.081e-2323.99roundabout guidance receptor 1 [Source:MGI Symbol;... [more]
Robo11.777e-2223.99roundabout guidance receptor 1 [Source:MGI Symbol;... [more]
Hmcn11.861e-2124.38hemicentin 1 [Source:MGI Symbol;Acc:MGI:2685047][more]
Hmcn12.293e-2124.38hemicentin 1 [Source:MGI Symbol;Acc:MGI:2685047][more]
Robo23.972e-2123.02roundabout guidance receptor 2 [Source:MGI Symbol;... [more]
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BLAST of hemicentin-1 isoform X2 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|O89026|ROBO1_MOUSE7.274e-2223.99Roundabout homolog 1 OS=Mus musculus OX=10090 GN=R... [more]
sp|Q9NR99|MXRA5_HUMAN8.470e-2222.70Matrix-remodeling-associated protein 5 OS=Homo sap... [more]
sp|Q9Y6N7|ROBO1_HUMAN2.528e-2123.57Roundabout homolog 1 OS=Homo sapiens OX=9606 GN=RO... [more]
sp|D3YXG0|HMCN1_MOUSE1.302e-2024.38Hemicentin-1 OS=Mus musculus OX=10090 GN=Hmcn1 PE=... [more]
sp|O55005|ROBO1_RAT1.684e-2023.66Roundabout homolog 1 OS=Rattus norvegicus OX=10116... [more]
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BLAST of hemicentin-1 isoform X2 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1S3HD631.089e-5825.67hemicentin-1 isoform X2 OS=Lingula unguis OX=7574 ... [more]
A0A1S3HD721.116e-5825.67hemicentin-1 isoform X1 OS=Lingula unguis OX=7574 ... [more]
A0A087U7N62.919e-5125.27Hemicentin-2 (Fragment) OS=Stegodyphus mimosarum O... [more]
A0A224Z0965.433e-5123.46Hemicentin-2-like OS=Rhipicephalus zambeziensis OX... [more]
A0A267F8R85.641e-5123.26Uncharacterized protein OS=Macrostomum lignano OX=... [more]
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BLAST of hemicentin-1 isoform X2 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSAMXT00000001796.21.591e-2323.03pep primary_assembly:Astyanax_mexicanus-2.0:20:237... [more]
robo14.987e-2222.24roundabout, axon guidance receptor, homolog 1 (Dro... [more]
robo26.765e-2222.82roundabout, axon guidance receptor, homolog 2 (Dro... [more]
robo19.593e-2222.85roundabout, axon guidance receptor, homolog 1 (Dro... [more]
robo13.850e-2122.58roundabout, axon guidance receptor, homolog 1 (Dro... [more]
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BLAST of hemicentin-1 isoform X2 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSPMAT00000010567.11.396e-1822.95pep scaffold:Pmarinus_7.0:GL479154:561817:685780:1... [more]
ENSPMAT00000008186.11.055e-1720.73pep scaffold:Pmarinus_7.0:GL476501:83598:168579:1 ... [more]
cntn3b2.668e-1220.83contactin 3b [Source:ZFIN;Acc:ZDB-GENE-130530-645][more]
ENSPMAT00000002502.12.467e-1122.11pep scaffold:Pmarinus_7.0:GL476400:249000:366561:-... [more]
pxdn5.422e-1021.12peroxidasin [Source:ZFIN;Acc:ZDB-GENE-140415-2][more]
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BLAST of hemicentin-1 isoform X2 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of hemicentin-1 isoform X2 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO319426.424e-1324.39Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO322972.085e-1125.10Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO322968.361e-1023.62Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO322992.162e-925.57Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO296163.056e-923.89Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of hemicentin-1 isoform X2 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSORLT00000012598.21.653e-1922.27roundabout homolog 1 [Source:NCBI gene;Acc:1011566... [more]
robo12.423e-1823.73roundabout guidance receptor 1 [Source:NCBI gene;A... [more]
ENSORLT00000042592.12.438e-1822.91roundabout homolog 2 [Source:NCBI gene;Acc:1011568... [more]
ENSORLT00000012625.23.571e-1823.24roundabout homolog 2 [Source:NCBI gene;Acc:1011568... [more]
igsf9bb4.550e-1822.80protein turtle homolog B-like [Source:NCBI gene;Ac... [more]
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BLAST of hemicentin-1 isoform X2 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000003113.10.000e+099.49SMESG000003113.1[more]
SMESG000003113.10.000e+099.49SMESG000003113.1[more]
SMESG000015102.12.431e-4724.27SMESG000015102.1[more]
SMESG000015102.12.702e-4724.16SMESG000015102.1[more]
SMESG000015102.14.064e-4724.16SMESG000015102.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30028839 ID=SMED30028839|Name=hemicentin-1 isoform X2|organism=Schmidtea mediterranea sexual|type=transcript|length=3766bp
TTAATTGACAATGATTTCGGGGAACTTTATCAACAGATAATGAAACTTAT
AACTTATTGTCAAATAGATAAAATATGGAATATTTCAAAACCTTTATTTT
AATAACATTATTTGGTAATCTGATGGACAGAAGTTACACTTTGAATTTTC
TCATTACCCCAAACAATGCCACAGTAAATAGTAAAGAAAACTTAACTCTT
GAATGCGTAGTAGATCAACAAAATATTGTTTACTCTTGGGAATATAAGCC
ATTTCATATTAAATCAAATATCATGTCTAAGGTTTTGGGATTTTGCTACT
TTGAAAAACCTTGCGTAGTTCCCGATTCGGAATCTCAAATCACAATCAGT
CACGAGAGCCGGCTAACAATCCACAATATATCCAGTGATAATTCAGGTTA
CTACGTTTGCAGAGTAACCTTACTTACCACGTCAGAGCACATAATATCCT
TAGCAGTTGTTACCGTAAAAACACCTCCAAAGTCAATAAGTTTTCATTTC
AACAAGTCTATCAATGTATTCCAAGAACAAGAATTTATTGCCATTCAATG
TTCTGTTTCAGGAGGTATACCACCTCCAAAGATTTCCATGTATATAGTAA
AAGATAATGCAATTAAACGTTTGAAAATCATTTCAAGCAATTTCCCAAAT
TCAACAATCGCGGAAGTAAGTGCAATATCTCTCTGGAGGCCTCACTATCT
TGAAAATAGAGGATTCGTAATGTGTACCGCAATAAATGATGCCGGAGTTT
TAACGAAAAATACTTCACTACTGAATATAAAATATAAACCAAAAATCAAA
CCATTTGTACCCAATACAGTGAACGCAATGATTAATCAGACAATAGATTT
GAGCTGTGAGGTTTACGCAAATCCAGCAGCAACAATCCAGTGGGTCATAA
TGCAAGGATTACAAATAATTGATTCCTCTAAATTTATAGCAAAAATCAGA
CCGGATATGGACGGACCACTTATTTGTGAAGCAAAAAATTATCTTGGTTC
AACTAAGGAGACAGTACAGTTAAATGTATTGAGGGATCCTCTAATTCATA
TCGATTCAAATTATCGTGTCCCAAAGAATTCAAATTATTCAATTAACTGC
CGCGTTACGTCGAATCCTCCCCCTGAATTTATCAAATGGTACAAACTATC
GAATATGACGGATTCTTCTGCGGTTTTGATTTCCGATGATGCCAAACTGA
ATTTCATTCTGATTGATAGGAGCTCAACTGGAATGTATGCCTGCGTAACT
CAAAATATTCAGATTTCAACGACGGGTAAAAGAAAACCGGCCAATTCAGA
AAAAACAATGTTTTTAACAGTTACTTACAAACCAAAGGAGCCACAGATTT
TGGGTAATAAAATAATTTCAATAATGAAAGGAGAAAAATTCATCATAAAA
TGCTCAACTGATCGAAAAAGTCCTGGTGAACCATTTCCTACACTTAAGTG
GATTGATGTGGAAACGAAAGATATTGTTTCAGACATGGAAACCTTTCAAG
TCATCAATTCAGAATCTCGACACAACGGAAAATACAAATGTTTAGCAGAA
AATGAAATAGGAAAGTCTTTTTCAAGAGAAACAGCTTTAGTCACAGTTAA
CGAAGCCCCATCAATTATTACGCAACACAAAAACAGAAAAGTATTCCTCA
GTTATTCTGACTTGCCACAATCAATTAGTTTCACCTGTGAATTTCGAGGC
CAGCCATTATATGAAGCTAGATGGCGTTATGGGAATGAAAAAATTCCTCC
TTCCTCTCAAGACTTTAAAATTGAAGATCATCGAAACATATCAAATGAAA
GAATATCACAGATTTTTACCAAGCTCACAATTGACCTTTATTCTCGGACA
TTTTCTCAGCAAACGTCTTTGAGATCATCGCCCAATGGTAGATACAATTG
TGAAGCAGATAACCGCCACGGAATAGCTTCTCTTGATTATTTTCTAATTA
TTAAATATCCTCCAATAATTAAATACAACATTCGCCATCGAAAGATTGCA
GCATCTTTAAATCAAAATGTCGTGATGATACGATGCCAGGCAGAATCTAT
ATCAACGATGCACATAAAATGGTATAAAACAAACGAGCATATTGTGTTAC
CTTTTCAAAGCAAGAAATACTCTATTTTTGAAGCGGAAGTTACAAAATAC
GAACCGGAAAGAGATTTATTTACTCCAGGTTTAAAAGTCAATGAACTAAC
TATAAAAGACTTAGAGAACGATGATTTTTCAAATTATTCTTGTGTTGCAT
CCAATGACCAAGGAATCAAAAAGGTCAATTTCGAAGTAGTATTTAAATCT
CAACCTGATCCACCATATGATTTGCGAGTAACCGACTTTGAATGGGATTC
TGTAACCATTAATTGGAAACCGGGTTTCAATGGAGGACTAACGCAAAATT
ATGAAATTTTAGTTATATCAGAGAATTTCGGTACTCAAAATGTACCATTT
TATTCAGATAAAAATGAAAACACTTTAACTATAACTGGACTTTATCCGAG
TACACCTTATGAAATCAGGATTTATGCAGTCAACTCTATCGGTCGAAGTC
TTAATTCAGAAACGGTAAAATGTGTCACGAAAAGAATTCGTAAATTCGAC
AAAATCCAAGCAAAAATTTTATCTGATCAACCAAAGATCGCAGTGATAAT
TCAAGAACCAGATGTATGTGCAAACCTTGAATACACAAGGGACAACGGGC
ACCAATGGTTTAGTATTTTACACTGTATTCGGTTCAATGCGTCTCATATT
AATGAAACGGTTCTCCTGGACATTATCCGGGTAGCGTCAATTCTGGATCT
CCAGTCAGGCTTAATTCATTTACGATTGACATTCTGTTTAATCAGTCGAC
CAAGTGTTTGTTCCCCTCACAGAATTCTTGAAAATCCAAAAAATGAAAAT
TTAGCTACACAAACGGTAATCTTAGTCATAACAGTGATCTGTTTTCTCCT
CATAATAATTTTGATCGCTCTATTTATCGTATGTTGTATCCGGTTCAGAT
ATCATTGTGTATCTTGTAATCAGTATTTTTGTCACAACTACTGTTGTTTT
CCTCGATTCTATGGTGCCACAAATAAACCGATTCGACATTACAACAACAA
TCGGATACCCTATGACGGAAAACCTTTGTCATGTAGCAGAGTCAGTTGTC
AGGATATACTTGATGAAAATGATATTAATACTGGTTCTACAAGTAACTAT
TGCTCAAGTTTGATTGGGTCTTCTACGCCTCATATTATGAACAAAAAATG
TCCTCAGAATTCCTCAAACGATGTTTATTCATCCTTTATTTGTCCTCATC
ATGAATTTCGTTCACATATTCCAGTACTATATGAAGGTAGTTCTTTTCTT
CAACCAACAGTGCTTTCTTGGTCTCAAAAAATAAGCCCTCCTTCTTGTGA
GGCTTCTTCAAGTCATTCTCACTCATCATCTACTGGAAGCAGTAGCAGGA
ATTTACCTCAAAATTCAGTACATTATAGAAAAAAACAAGACGGCATATCA
AATAGTAATGTGGTTAATCATAAATCATATGCTCAAACTGAAGGAATTAT
CAAGTTGCCTGATGTCACTTCAGAAGCCATTCATTTTGTCAAGCCTGATT
ATAATGAAAAATTAATGGAGGCAGTTGTATAGAAATTTGTTGAATGATTT
GTCATTTTTGGTGAAACTGCTATTTTGTGTGATTTTCAAAGGCTTTTACA
GTTGATCATTTGAGAAAGTTTATAGAATACAGGTTAATGCTGCATTTAAT
TTGAGAGATTATTAAT
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protein sequence of SMED30028839-orf-1

>SMED30028839-orf-1 ID=SMED30028839-orf-1|Name=SMED30028839-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=1186bp
MEYFKTFILITLFGNLMDRSYTLNFLITPNNATVNSKENLTLECVVDQQN
IVYSWEYKPFHIKSNIMSKVLGFCYFEKPCVVPDSESQITISHESRLTIH
NISSDNSGYYVCRVTLLTTSEHIISLAVVTVKTPPKSISFHFNKSINVFQ
EQEFIAIQCSVSGGIPPPKISMYIVKDNAIKRLKIISSNFPNSTIAEVSA
ISLWRPHYLENRGFVMCTAINDAGVLTKNTSLLNIKYKPKIKPFVPNTVN
AMINQTIDLSCEVYANPAATIQWVIMQGLQIIDSSKFIAKIRPDMDGPLI
CEAKNYLGSTKETVQLNVLRDPLIHIDSNYRVPKNSNYSINCRVTSNPPP
EFIKWYKLSNMTDSSAVLISDDAKLNFILIDRSSTGMYACVTQNIQISTT
GKRKPANSEKTMFLTVTYKPKEPQILGNKIISIMKGEKFIIKCSTDRKSP
GEPFPTLKWIDVETKDIVSDMETFQVINSESRHNGKYKCLAENEIGKSFS
RETALVTVNEAPSIITQHKNRKVFLSYSDLPQSISFTCEFRGQPLYEARW
RYGNEKIPPSSQDFKIEDHRNISNERISQIFTKLTIDLYSRTFSQQTSLR
SSPNGRYNCEADNRHGIASLDYFLIIKYPPIIKYNIRHRKIAASLNQNVV
MIRCQAESISTMHIKWYKTNEHIVLPFQSKKYSIFEAEVTKYEPERDLFT
PGLKVNELTIKDLENDDFSNYSCVASNDQGIKKVNFEVVFKSQPDPPYDL
RVTDFEWDSVTINWKPGFNGGLTQNYEILVISENFGTQNVPFYSDKNENT
LTITGLYPSTPYEIRIYAVNSIGRSLNSETVKCVTKRIRKFDKIQAKILS
DQPKIAVIIQEPDVCANLEYTRDNGHQWFSILHCIRFNASHINETVLLDI
IRVASILDLQSGLIHLRLTFCLISRPSVCSPHRILENPKNENLATQTVIL
VITVICFLLIIILIALFIVCCIRFRYHCVSCNQYFCHNYCCFPRFYGATN
KPIRHYNNNRIPYDGKPLSCSRVSCQDILDENDINTGSTSNYCSSLIGSS
TPHIMNKKCPQNSSNDVYSSFICPHHEFRSHIPVLYEGSSFLQPTVLSWS
QKISPPSCEASSSHSHSSSTGSSSRNLPQNSVHYRKKQDGISNSNVVNHK
SYAQTEGIIKLPDVTSEAIHFVKPDYNEKLMEAVV*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000020protonephridia
PLANA:0000101muscle cell
PLANA:0000106ciliated epithelial cell
PLANA:0000418head
Vocabulary: INTERPRO
TermDefinition
IPR036179Ig-like_dom_sf
IPR036116FN3_sf
IPR003598Ig_sub2
IPR003961FN3_dom
IPR003599Ig_sub
IPR013783Ig-like_fold
IPR007110Ig-like_dom
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003961Fibronectin type IIISMARTSM00060FN3_2coord: 744..825
e-value: 2.1E-12
score: 57.2
IPR003961Fibronectin type IIIPFAMPF00041fn3coord: 745..825
e-value: 3.7E-12
score: 46.4
IPR003961Fibronectin type IIIPROSITEPS50853FN3coord: 746..838
score: 19.74
IPR003961Fibronectin type IIICDDcd00063FN3coord: 744..835
e-value: 2.03148E-16
score: 73.6847
IPR003598Immunoglobulin subtype 2SMARTSM00408igc2_5coord: 434..496
e-value: 0.59
score: 9.3
coord: 529..616
e-value: 0.38
score: 11.2
coord: 644..730
e-value: 0.078
score: 17.7
coord: 333..397
e-value: 0.8
score: 8.1
coord: 35..119
e-value: 0.15
score: 15.0
coord: 252..308
e-value: 4.1
score: 1.3
IPR003599Immunoglobulin subtypeSMARTSM00409IG_3ccoord: 428..509
e-value: 0.0017
score: 27.6
coord: 638..741
e-value: 1.0
score: 18.4
coord: 327..417
e-value: 4.7
score: 14.3
coord: 246..319
e-value: 0.0058
score: 25.8
coord: 29..132
e-value: 4.8E-7
score: 39.4
IPR013783Immunoglobulin-like foldGENE3DG3DSA:2.60.40.10coord: 742..839
e-value: 2.6E-17
score: 65.0
IPR013783Immunoglobulin-like foldGENE3DG3DSA:2.60.40.10coord: 323..426
e-value: 1.5E-9
score: 39.7
IPR013783Immunoglobulin-like foldGENE3DG3DSA:2.60.40.10coord: 19..141
e-value: 1.3E-10
score: 43.2
IPR013783Immunoglobulin-like foldGENE3DG3DSA:2.60.40.10coord: 142..243
e-value: 9.7E-7
score: 31.0
IPR013783Immunoglobulin-like foldGENE3DG3DSA:2.60.40.10coord: 427..508
e-value: 1.8E-7
score: 32.9
IPR013783Immunoglobulin-like foldGENE3DG3DSA:2.60.40.10coord: 509..633
e-value: 4.0E-10
score: 41.6
coord: 634..741
e-value: 1.1E-10
score: 43.5
IPR013783Immunoglobulin-like foldGENE3DG3DSA:2.60.40.10coord: 244..320
e-value: 4.1E-9
score: 38.4
NoneNo IPR availablePFAMPF13927Ig_3coord: 629..727
e-value: 5.4E-7
score: 30.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1108..1129
NoneNo IPR availablePANTHERPTHR12231CTX-RELATED TYPE I TRANSMEMBRANE PROTEINcoord: 533..844
coord: 213..519
coord: 28..359
NoneNo IPR availableCDDcd00096Igcoord: 534..619
e-value: 2.46104E-4
score: 38.7625
NoneNo IPR availableCDDcd00096Igcoord: 339..400
e-value: 1.58145E-4
score: 39.1477
NoneNo IPR availableTMHMMTMhelixcoord: 947..969
IPR007110Immunoglobulin-like domainPROSITEPS50835IG_LIKEcoord: 23..125
score: 8.974
IPR007110Immunoglobulin-like domainPROSITEPS50835IG_LIKEcoord: 239..317
score: 8.284
IPR007110Immunoglobulin-like domainPROSITEPS50835IG_LIKEcoord: 420..507
score: 9.682
IPR007110Immunoglobulin-like domainPROSITEPS50835IG_LIKEcoord: 322..409
score: 10.208
IPR007110Immunoglobulin-like domainPROSITEPS50835IG_LIKEcoord: 629..739
score: 10.335
IPR007110Immunoglobulin-like domainPROSITEPS50835IG_LIKEcoord: 512..613
score: 7.177
IPR036179Immunoglobulin-like domain superfamilySUPERFAMILYSSF48726Immunoglobulincoord: 429..516
IPR036179Immunoglobulin-like domain superfamilySUPERFAMILYSSF48726Immunoglobulincoord: 24..136
IPR036179Immunoglobulin-like domain superfamilySUPERFAMILYSSF48726Immunoglobulincoord: 134..239
IPR036179Immunoglobulin-like domain superfamilySUPERFAMILYSSF48726Immunoglobulincoord: 327..399
IPR036179Immunoglobulin-like domain superfamilySUPERFAMILYSSF48726Immunoglobulincoord: 627..747
IPR036179Immunoglobulin-like domain superfamilySUPERFAMILYSSF48726Immunoglobulincoord: 532..630
IPR036179Immunoglobulin-like domain superfamilySUPERFAMILYSSF48726Immunoglobulincoord: 236..319
IPR036116Fibronectin type III superfamilySUPERFAMILYSSF49265Fibronectin type IIIcoord: 733..836