TUB like protein 4

Overview
NameTUB like protein 4
Smed IDSMED30028792
Length (bp)3160
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of TUB like protein 4 (SMED30028792) t-SNE clustered cells

Violin plots show distribution of expression levels for TUB like protein 4 (SMED30028792) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of TUB like protein 4 (SMED30028792) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for TUB like protein 4 (SMED30028792) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30028792

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30028792h1SMcG0006319 SmedASXL_013280SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
nervous systemSMED30028792h1SMcG0006319 dd_Smed_v4_18217_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30028792h1SMcG0006319 dd_Smed_v4_18217_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30028792h1SMcG0006319 dd_Smed_v4_18217_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30028792h1SMcG0006319 dd_Smed_v6_18217_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of TUB like protein 4 vs. Ensembl Human
Match: TULP4 (TUB like protein 4 [Source:HGNC Symbol;Acc:HGNC:15530])

HSP 1 Score: 127.102 bits (318), Expect = 2.282e-29
Identity = 120/510 (23.53%), Postives = 216/510 (42.35%), Query Frame = 3
Query: 1011 LTCGTWSPNDATIIVGTSIGSLLSFPYAWEXXXXXXXXXXXXXXXXNDSILQVEWSCPPFRRNENGGESITNQDQKYLINSNMKYPHNSFPDSEIPLNDSAASSDLENQLFDNSENFLLAVAFSSGYIYLLRHLEDVDPIKHQTGLSRLCINWNSTGTILAVGGFVRSANLECQCSVKLLSTSAEIIY-------FVPVPSMNKPIMSVTWGHEDTRLFVSASICVHTFRVSSSFSSLTQRCCHIIRNSLKFRQESVDKLCLPSYLHKKIHNSFRKTIQGSIPLPFRLKEFVLLPKQ--DVLHCIFQKVSTGN--GLTKYILYLEFLGASIPLLTASRSRSVKYEYHIYDPSLNQVVDSDPLFPNEEME--VEHSNCTSQC------VWQRKFKHQNRRKTQNCGRNSISVLRDTDSNFSQFLRQESGKIDNCIDSKRSRLNELMTNDDLP-----ENCLLAKLKSNVTLTKFELTSCQICLPRILSRINFGVNIKLNPSNGVSVHLVEI 2468
            +TCG W+P+D  ++ GT+ G ++                   LL  +D +L + W+ P F         + +  +    + +   P +      IP+ +                  LL V+F+SG I L+ + +D+ P   ++GL  +   W + G +LAV G  R   L    +  LL ++    Y       F     + +PI+S+ WGH D+RL +++   ++  RV    SSL   C   I ++L+   + V KL LP  L   +  +F  TI+  IP P  +++FV  P    + LHC  ++       G   Y LYLE+LG  +P+L   R   ++ E+ I DP  +     D ++ N  +   ++  NC+          W  K   +  R +++     IS+         +   QE  +       K S  +  +T  + P     ++  LA++ SN+  TKF++      LP  L  + +  ++       ++++L E+
Sbjct:  170 ITCGIWTPDDQQVLFGTADGQVIVM-------DCHGRMLAHVLLHESDGVLGMSWNYPIFL--------VEDSSESDTDSDDYAPPQDGPAAYPIPVQNIKP---------------LLTVSFTSGDISLMNNYDDLSPTVIRSGLKEVVAQWCTQGDLLAVAGMERQTQLGELPNGPLLKSAMVKFYNVRGEHIFTLDTLVQRPIISICWGHRDSRLLMASGPALYVVRVEHRVSSLQLLCQQAIASTLR-EDKDVSKLTLPPRLCSYLSTAFIPTIKPPIPDPNNMRDFVSYPSAGNERLHCTMKRTEDDPEVGGPCYTLYLEYLGGLVPILKGRRISKLRPEFVIMDPRTDS--KPDEIYGNSLISTVIDSCNCSDSSDIELSDDWAAKKSPKISRASKSPKLPRISIEARKSPKLPR-AAQELSRSPRLPLRKPSVGSPSLTRREFPFEDITQHNYLAQVTSNIWGTKFKIVGLAAFLPTNLGAVIYKTSLLHLQPRQMTIYLPEV 645          

HSP 2 Score: 104.76 bits (260), Expect = 2.907e-22
Identity = 53/135 (39.26%), Postives = 72/135 (53.33%), Query Frame = 3
Query:  219 GWLACGNKTGSVSITKTM--IESDDSTESIPYELPARTNYNLRNSKVPINIVEWNPHHKKLATCSNTGSILVYHNVNNQWSIDLVNERAHAVEVFKWSRSTNQVMIGYTDGLLIIGSVSGHRLWHRMMDMHSALN 617
            GWLA GN  G V +T T      D ST       P R N+NLR     + +V WN  ++KLATC   G I V+     +WS++LVN+R   V  F WS    Q +I Y DG +++GSVSG R W   +++ S + 
Sbjct:   44 GWLATGNGRGVVGVTFTSSHCRRDRST-------PQRINFNLRGHNSEVVLVRWNEPYQKLATCDADGGIFVWIQYEGRWSVELVNDRGAQVSDFTWSHDGTQALISYRDGFVLVGSVSGQRHWSSEINLESQIT 171          
BLAST of TUB like protein 4 vs. Ensembl Human
Match: TULP4 (TUB like protein 4 [Source:HGNC Symbol;Acc:HGNC:15530])

HSP 1 Score: 127.872 bits (320), Expect = 4.614e-29
Identity = 124/511 (24.27%), Postives = 218/511 (42.66%), Query Frame = 3
Query: 1011 LTCGTWSPNDATIIVGTSIGSLLSFPYAWEXXXXXXXXXXXXXXXXNDSILQVEWSCPPFRRNENGGESITNQDQKYLINSNMKYPHNSFPDSEIPLNDSAASSDLENQLFDNSENFLLAVAFSSGYIYLLRHLEDVDPIKHQTGLSRLCINWNSTGTILAVGGFVRSANLECQCSVKLLSTSAEIIY-------FVPVPSMNKPIMSVTWGHEDTRLFVSASICVHTFRVSSSFSSLTQRCCHIIRNSLKFRQESVDKLCLPSYLHKKIHNSFRKTIQGSIPLPFRLKEFVLLPKQ--DVLHCIFQKVSTGN--GLTKYILYLEFLGASIPLLTASRSRSVKYEYHIYDPSLNQVVDSDPLFPNEEME--VEHSNCTSQC------VWQRKFKHQNRRKTQNCGRNSISVLRDTDSNFSQFLRQESGKIDNCIDSKRSRLNELMTNDDLP-----ENCLLAKLKSNVTLTKFELTSCQICLPRILSRINFGVN-IKLNPSNGVSVHLVEI 2468
            +TCG W+P+D  ++ GT+ G ++                   LL  +D +L + W+ P F   ++      + D     +    YP        IP+ +                  LL V+F+SG I L+ + +D+ P   ++GL  +   W + G +LAV G  R   L    +  LL ++    Y       F     + +PI+S+ WGH D+RL +++   ++  RV    SSL   C   I ++L+   + V KL LP  L   +  +F  TI+  IP P  +++FV  P    + LHC  ++       G   Y LYLE+LG  +P+L   R   ++ E+ I DP  +     D ++ N  +   ++  NC+          W  K   +  R +++     IS+         +   QE  +       K S  +  +T  + P     ++  LA++ SN+  TKF++      LP  L  + +  + + L P   ++++L E+
Sbjct:  170 ITCGIWTPDDQQVLFGTADGQVIVM-------DCHGRMLAHVLLHESDGVLGMSWNYPIFLVEDSSESDTDSDDYAPPQDGPAAYP--------IPVQNIKP---------------LLTVSFTSGDISLMNNYDDLSPTVIRSGLKEVVAQWCTQGDLLAVAGMERQTQLGELPNGPLLKSAMVKFYNVRGEHIFTLDTLVQRPIISICWGHRDSRLLMASGPALYVVRVEHRVSSLQLLCQQAIASTLR-EDKDVSKLTLPPRLCSYLSTAFIPTIKPPIPDPNNMRDFVSYPSAGNERLHCTMKRTEDDPEVGGPCYTLYLEYLGGLVPILKGRRISKLRPEFVIMDPRTDS--KPDEIYGNSLISTVIDSCNCSDSSDIELSDDWAAKKSPKISRASKSPKLPRISIEARKSPKLPR-AAQELSRSPRLPLRKPSVGSPSLTRREFPFEDITQHNYLAQVTSNIWGTKFKIVGLAAFLPTNLGAVIYKTSLLHLQPRQ-MTIYLPEV 645          

HSP 2 Score: 105.916 bits (263), Expect = 2.696e-22
Identity = 53/134 (39.55%), Postives = 72/134 (53.73%), Query Frame = 3
Query:  219 GWLACGNKTGSVSITKTM--IESDDSTESIPYELPARTNYNLRNSKVPINIVEWNPHHKKLATCSNTGSILVYHNVNNQWSIDLVNERAHAVEVFKWSRSTNQVMIGYTDGLLIIGSVSGHRLWHRMMDMHSAL 614
            GWLA GN  G V +T T      D ST       P R N+NLR     + +V WN  ++KLATC   G I V+     +WS++LVN+R   V  F WS    Q +I Y DG +++GSVSG R W   +++ S +
Sbjct:   44 GWLATGNGRGVVGVTFTSSHCRRDRST-------PQRINFNLRGHNSEVVLVRWNEPYQKLATCDADGGIFVWIQYEGRWSVELVNDRGAQVSDFTWSHDGTQALISYRDGFVLVGSVSGQRHWSSEINLESQI 170          

HSP 3 Score: 53.1434 bits (126), Expect = 3.011e-6
Identity = 20/52 (38.46%), Postives = 36/52 (69.23%), Query Frame = 3
Query: 2931 HSSKRIILKYDNDQILDFSRLENDKYSLEFKYPFSPLQAFAVAIANISRLFR 3086
             S+K   ++ +  Q++ F R++   Y L+F+YPFS +QAFAVA+AN+++  +
Sbjct: 1492 ESAKNFQIELEGRQVMQFGRIDGSAYILDFQYPFSAVQAFAVALANVTQRLK 1543          
BLAST of TUB like protein 4 vs. Ensembl Human
Match: TULP4 (TUB like protein 4 [Source:HGNC Symbol;Acc:HGNC:15530])

HSP 1 Score: 87.8113 bits (216), Expect = 9.042e-19
Identity = 50/150 (33.33%), Postives = 79/150 (52.67%), Query Frame = 3
Query: 1593 MNKPIMSVTWGHEDTRLFVSASICVHTFRVSSSFSSLTQRCCHIIRNSLKFRQESVDKLCLPSYLHKKIHNSFRKTIQGSIPLPFRLKEFVLLPKQ--DVLHCIFQKVSTGN--GLTKYILYLEFLGASIPLLTASRSRSVKYEYHIYDP 2030
            + +PI+S+ WGH D+RL +++   ++  RV    SSL   C   I ++L+   + V KL LP  L   +  +F  TI+  IP P  +++FV  P    + LHC  ++       G   Y LYLE+LG  +P+L   R   ++ E+ I DP
Sbjct:   26 VQRPIISICWGHRDSRLLMASGPALYVVRVEHRVSSLQLLCQQAIASTLR-EDKDVSKLTLPPRLCSYLSTAFIPTIKPPIPDPNNMRDFVSYPSAGNERLHCTMKRTEDDPEVGGPCYTLYLEYLGGLVPILKGRRISKLRPEFVIMDP 174          
BLAST of TUB like protein 4 vs. Ensembl Human
Match: WDR35 (WD repeat domain 35 [Source:HGNC Symbol;Acc:HGNC:29250])

HSP 1 Score: 68.9366 bits (167), Expect = 4.769e-11
Identity = 37/128 (28.91%), Postives = 63/128 (49.22%), Query Frame = 3
Query:  219 GWLACGNKTGSVSITKTMIESDDSTE---SIPYELPARTNYNLRNSKVPINIVEWNPHHKKLATCSNTGSILVYHNVNNQWSIDLVNER-AHAVEVFKWSRSTNQVMIGYTDGLLIIGSVSGHRLWHR 590
            G++ACG + G + + K   ++DD+     + P  L    N  L      + +V WN  ++KL T    G I+V+      W  +++N R    V    W+    ++ I Y DG +I+GSV G+R+W +
Sbjct:   27 GFIACGGEDGLLKVLKLETQTDDAKLRGLAAPSNLS--MNQTLEGHSGSVQVVTWNEQYQKLTTSDENGLIIVWMLYKGSWIEEMINNRNKSVVRSMSWNADGQKICIVYEDGAVIVGSVDGNRIWGK 152          
BLAST of TUB like protein 4 vs. Ensembl Human
Match: WDR35 (WD repeat domain 35 [Source:HGNC Symbol;Acc:HGNC:29250])

HSP 1 Score: 68.9366 bits (167), Expect = 5.052e-11
Identity = 37/128 (28.91%), Postives = 63/128 (49.22%), Query Frame = 3
Query:  219 GWLACGNKTGSVSITKTMIESDDSTE---SIPYELPARTNYNLRNSKVPINIVEWNPHHKKLATCSNTGSILVYHNVNNQWSIDLVNER-AHAVEVFKWSRSTNQVMIGYTDGLLIIGSVSGHRLWHR 590
            G++ACG + G + + K   ++DD+     + P  L    N  L      + +V WN  ++KL T    G I+V+      W  +++N R    V    W+    ++ I Y DG +I+GSV G+R+W +
Sbjct:   27 GFIACGGEDGLLKVLKLETQTDDAKLRGLAAPSNLS--MNQTLEGHSGSVQVVTWNEQYQKLTTSDENGLIIVWMLYKGSWIEEMINNRNKSVVRSMSWNADGQKICIVYEDGAVIVGSVDGNRIWGK 152          
BLAST of TUB like protein 4 vs. Ensembl Celegans
Match: tub-2 (TUBby-related [Source:UniProtKB/TrEMBL;Acc:Q9BL53])

HSP 1 Score: 90.5077 bits (223), Expect = 3.801e-18
Identity = 51/149 (34.23%), Postives = 75/149 (50.34%), Query Frame = 3
Query:  174 ILSTSWMQDHTHSNIGWLACGNKTGSVSITKTMIES--DDSTESIPYELPARTNYNLRNSKVPINIVEWNPHHKKLATCSNTGSILVYHNVNNQWSIDLVNERAHAVEVFKWSRSTNQVMIGYTDGLLIIGSVSGHRLWHRMMDMHSAL 614
            I   SW+Q     + G L  G +TGSV +T T  +   DD T         R N+NLR     I +V WN    KLA+C  +G I V+   +++WS++LVN+R   V    WS      +I Y D  ++IGS +G R+W     + +A+
Sbjct:   18 ITHLSWIQQQDKPDHGLLGVGTETGSVGVTLTDFKPTPDDLT---------RYNFNLRGHHNAIRMVTWNKSQSKLASCDASGIIYVWVRNDDRWSVELVNDRGVKVSDLSWSPCGGSALICYDDNFVLIGSANGQRVWSNSFPLPAAV 157          
BLAST of TUB like protein 4 vs. Ensembl Celegans
Match: ifta-1 (IntraFlagellar Transport Associated [Source:UniProtKB/TrEMBL;Acc:Q18859])

HSP 1 Score: 72.7886 bits (177), Expect = 1.210e-12
Identity = 37/134 (27.61%), Postives = 69/134 (51.49%), Query Frame = 3
Query:  213 NIGWLACGNKTGSVSITKTMIESDDSTESIPYELPARTNYNLRNSKVPINIVEWNPHHKKLATCSNTGSILVYHNVNNQWSIDLVNERAHAVEV-FKWSRSTNQVMIGYTDGLLIIGSVSGHRLWHRMMDMHSA 611
            N  ++ACG   G + + K   ++ D  +S P       N  L      +    WN +++KL T   +G I+V+   N QW  +++N R  +V V   W+    ++ I Y DG +I+G++ G+R+W++ +++  A
Sbjct:   45 NSNYIACGGALGKLKVVKIGTDATDLNKS-PNAATLVVNQALEGHNATVMNATWNENNQKLTTSDTSGLIIVWGMFNEQWCEEMINNRNKSVVVSICWNLEGTKIAIAYADGNVIVGTLEGNRIWNKELEIQLA 177          
BLAST of TUB like protein 4 vs. Ensembl Fly
Match: Tusp (gene:FBgn0039530 transcript:FBtr0335246)

HSP 1 Score: 132.109 bits (331), Expect = 1.151e-30
Identity = 136/517 (26.31%), Postives = 215/517 (41.59%), Query Frame = 3
Query: 1008 TLTCGTWSPNDATIIVGTSIGSLLSFPYAWEXXXXXXXXXXXXXXXXND-SILQVEWSCPPFRRNENGGESITNQDQKYLINSNMKYPHNSFPDSEIPLNDSAASSDLENQLFDNSENFLLAVAFSSGYIYLLRHLEDVDPIKHQT---GLSRLCINWNSTGTILAVGGFVRSA----------NLECQCS-VKLLSTSAEIIYFVPVPSMNKPIMSVTWGHEDTRLFVSASICVHTFRVSSSFSSLTQRCCHIIRNSLKFRQESVDKLCLPSYLHKKIHNSFRKTIQGSIPLPFRLKEFVLLPK--QDVLHCIFQKVSTGNGL---TKYILYLEFLGASIPLLTASRSRSVKYEYHIYDPSLNQVVDSDPLFPNEEMEVEHSNCTSQCVWQRKFKHQNRRKTQNCGRNSISVLRDTDSNF---SQF----------------------LRQESGKIDNCIDSKRSRLNELMTNDDLPENCLLAKLKSNVTLTKFELTSCQICLPRILSRINFGVNI 2423
            T+TCG W+P+D  +  GT+ G ++                  ++   ND  I  + WSC  F+  E  GE                       ++E  + ++A  S            F+LAV+F +GYIYLL+  +DV P    T   G   + + W+++  +LAV G +R+              C  + VK  + +   +Y   +P  +  + ++TWGH D RLF++    VH   VS   +SL   C   I+ S+   +  +  L LPS +   I N F +TI+  +P    L++FV  P      LHC   +    + L   T Y LYLE+LG  +PLL   R+  ++ E+ I+DP +N      PL+     E + S+                  T N GR   S     DS+F   S+F                      L    G + N  DS    L+EL   D LPE   L ++ SN+  TKF++      +P  L ++ +  ++
Sbjct:  172 TITCGIWTPDDQQVYFGTTQGQVIVM--------DVHGAMVSQVQLSNDVPITSMAWSCEKFKMEE--GE-----------------------EAEPGVTNAAKRS------------FVLAVSFQNGYIYLLKSFDDVSPAHINTCLNGALGMVMEWSNSRELLAVAGTLRTGVDGTKTEDMGTPSCYTNLVKFYTETGTCLYQAHIPCSSATVSAITWGHNDKRLFIATGTQVHIAWVSRRVASLQLLCRLEIQASVG-SESLLPLLPLPSRIKSLIGNLFAQTIRCCVPDLKSLRDFVSRPPLCSTRLHCTMIRHDDDSNLSSGTCYTLYLEYLGGLVPLLKGKRTSKIRPEFVIFDPQVND----SPLYFQYSAEAKSSS-----------GSSQSTTTGNSGRTDSS-----DSDFEERSRFGSPRTPRKKRVRPKRRHQAGDRLSASGGGVSNDPDS----LDELAYVDTLPEEVKLVEVTSNIWGTKFKIHGLAKTVPANLGQVTYKTSL 618          

HSP 2 Score: 106.301 bits (264), Expect = 8.234e-23
Identity = 50/132 (37.88%), Postives = 76/132 (57.58%), Query Frame = 3
Query:  219 GWLACGNKTGSVSITKTMIESDDSTESIPYELPARTNYNLRNSKVPINIVEWNPHHKKLATCSNTGSILVYHNVNNQWSIDLVNERAHAVEVFKWSRSTNQVMIGYTDGLLIIGSVSGHRLWHRMMDMHSAL 614
            GWLA GN  G V +T T      +      + P RTNYNLR  +  + +V+WN  ++KLA+C ++G I V+     +WSI+L+N+R   V  F WS      +I Y DG +++GSV+G R W  M+++ S +
Sbjct:   47 GWLATGNVRGIVGVTFTTSHCRKN-----MDYPLRTNYNLRGHRSDVILVKWNEPYQKLASCDSSGIIFVWIKYEGRWSIELINDRNTPVTHFSWSHDGRMALICYQDGFVLVGSVAGQRYWSSMLNLESTI 173          

HSP 3 Score: 57.3806 bits (137), Expect = 7.688e-8
Identity = 28/86 (32.56%), Postives = 47/86 (54.65%), Query Frame = 3
Query: 2862 PIPSFRTFFVSNE-----------QKDSKHDRYLHSSKRIILKYDNDQILDFSRLENDKYSLEFKYPFSPLQAFAVAIANISRLFR 3086
            P P  R F + N+           Q D        S+K   +++   Q++ F R++ + Y+L+F+YPFS LQAFAVA+AN+++  +
Sbjct: 1345 PAPVRREFVMHNKAPMWNEMSQVYQLDFGGRVTQESAKNFQIEFRGKQVMQFGRIDGNAYTLDFQYPFSALQAFAVALANVTQRLK 1430          
BLAST of TUB like protein 4 vs. Ensembl Fly
Match: Tusp (gene:FBgn0039530 transcript:FBtr0302589)

HSP 1 Score: 131.724 bits (330), Expect = 1.266e-30
Identity = 136/517 (26.31%), Postives = 215/517 (41.59%), Query Frame = 3
Query: 1008 TLTCGTWSPNDATIIVGTSIGSLLSFPYAWEXXXXXXXXXXXXXXXXND-SILQVEWSCPPFRRNENGGESITNQDQKYLINSNMKYPHNSFPDSEIPLNDSAASSDLENQLFDNSENFLLAVAFSSGYIYLLRHLEDVDPIKHQT---GLSRLCINWNSTGTILAVGGFVRSA----------NLECQCS-VKLLSTSAEIIYFVPVPSMNKPIMSVTWGHEDTRLFVSASICVHTFRVSSSFSSLTQRCCHIIRNSLKFRQESVDKLCLPSYLHKKIHNSFRKTIQGSIPLPFRLKEFVLLPK--QDVLHCIFQKVSTGNGL---TKYILYLEFLGASIPLLTASRSRSVKYEYHIYDPSLNQVVDSDPLFPNEEMEVEHSNCTSQCVWQRKFKHQNRRKTQNCGRNSISVLRDTDSNF---SQF----------------------LRQESGKIDNCIDSKRSRLNELMTNDDLPENCLLAKLKSNVTLTKFELTSCQICLPRILSRINFGVNI 2423
            T+TCG W+P+D  +  GT+ G ++                  ++   ND  I  + WSC  F+  E  GE                       ++E  + ++A  S            F+LAV+F +GYIYLL+  +DV P    T   G   + + W+++  +LAV G +R+              C  + VK  + +   +Y   +P  +  + ++TWGH D RLF++    VH   VS   +SL   C   I+ S+   +  +  L LPS +   I N F +TI+  +P    L++FV  P      LHC   +    + L   T Y LYLE+LG  +PLL   R+  ++ E+ I+DP +N      PL+     E + S+                  T N GR   S     DS+F   S+F                      L    G + N  DS    L+EL   D LPE   L ++ SN+  TKF++      +P  L ++ +  ++
Sbjct:  172 TITCGIWTPDDQQVYFGTTQGQVIVM--------DVHGAMVSQVQLSNDVPITSMAWSCEKFKMEE--GE-----------------------EAEPGVTNAAKRS------------FVLAVSFQNGYIYLLKSFDDVSPAHINTCLNGALGMVMEWSNSRELLAVAGTLRTGVDGTKTEDMGTPSCYTNLVKFYTETGTCLYQAHIPCSSATVSAITWGHNDKRLFIATGTQVHIAWVSRRVASLQLLCRLEIQASVG-SESLLPLLPLPSRIKSLIGNLFAQTIRCCVPDLKSLRDFVSRPPLCSTRLHCTMIRHDDDSNLSSGTCYTLYLEYLGGLVPLLKGKRTSKIRPEFVIFDPQVND----SPLYFQYSAEAKSSS-----------GSSQSTTTGNSGRTDSS-----DSDFEERSRFGSPRTPRKKRVRPKRRHQAGDRLSASGGGVSNDPDS----LDELAYVDTLPEEVKLVEVTSNIWGTKFKIHGLAKTVPANLGQVTYKTSL 618          

HSP 2 Score: 106.301 bits (264), Expect = 8.310e-23
Identity = 50/132 (37.88%), Postives = 76/132 (57.58%), Query Frame = 3
Query:  219 GWLACGNKTGSVSITKTMIESDDSTESIPYELPARTNYNLRNSKVPINIVEWNPHHKKLATCSNTGSILVYHNVNNQWSIDLVNERAHAVEVFKWSRSTNQVMIGYTDGLLIIGSVSGHRLWHRMMDMHSAL 614
            GWLA GN  G V +T T      +      + P RTNYNLR  +  + +V+WN  ++KLA+C ++G I V+     +WSI+L+N+R   V  F WS      +I Y DG +++GSV+G R W  M+++ S +
Sbjct:   47 GWLATGNVRGIVGVTFTTSHCRKN-----MDYPLRTNYNLRGHRSDVILVKWNEPYQKLASCDSSGIIFVWIKYEGRWSIELINDRNTPVTHFSWSHDGRMALICYQDGFVLVGSVAGQRYWSSMLNLESTI 173          

HSP 3 Score: 57.3806 bits (137), Expect = 7.755e-8
Identity = 28/86 (32.56%), Postives = 47/86 (54.65%), Query Frame = 3
Query: 2862 PIPSFRTFFVSNE-----------QKDSKHDRYLHSSKRIILKYDNDQILDFSRLENDKYSLEFKYPFSPLQAFAVAIANISRLFR 3086
            P P  R F + N+           Q D        S+K   +++   Q++ F R++ + Y+L+F+YPFS LQAFAVA+AN+++  +
Sbjct: 1347 PAPVRREFVMHNKAPMWNEMSQVYQLDFGGRVTQESAKNFQIEFRGKQVMQFGRIDGNAYTLDFQYPFSALQAFAVALANVTQRLK 1432          
BLAST of TUB like protein 4 vs. Ensembl Fly
Match: Tusp (gene:FBgn0039530 transcript:FBtr0302590)

HSP 1 Score: 131.724 bits (330), Expect = 1.266e-30
Identity = 136/517 (26.31%), Postives = 215/517 (41.59%), Query Frame = 3
Query: 1008 TLTCGTWSPNDATIIVGTSIGSLLSFPYAWEXXXXXXXXXXXXXXXXND-SILQVEWSCPPFRRNENGGESITNQDQKYLINSNMKYPHNSFPDSEIPLNDSAASSDLENQLFDNSENFLLAVAFSSGYIYLLRHLEDVDPIKHQT---GLSRLCINWNSTGTILAVGGFVRSA----------NLECQCS-VKLLSTSAEIIYFVPVPSMNKPIMSVTWGHEDTRLFVSASICVHTFRVSSSFSSLTQRCCHIIRNSLKFRQESVDKLCLPSYLHKKIHNSFRKTIQGSIPLPFRLKEFVLLPK--QDVLHCIFQKVSTGNGL---TKYILYLEFLGASIPLLTASRSRSVKYEYHIYDPSLNQVVDSDPLFPNEEMEVEHSNCTSQCVWQRKFKHQNRRKTQNCGRNSISVLRDTDSNF---SQF----------------------LRQESGKIDNCIDSKRSRLNELMTNDDLPENCLLAKLKSNVTLTKFELTSCQICLPRILSRINFGVNI 2423
            T+TCG W+P+D  +  GT+ G ++                  ++   ND  I  + WSC  F+  E  GE                       ++E  + ++A  S            F+LAV+F +GYIYLL+  +DV P    T   G   + + W+++  +LAV G +R+              C  + VK  + +   +Y   +P  +  + ++TWGH D RLF++    VH   VS   +SL   C   I+ S+   +  +  L LPS +   I N F +TI+  +P    L++FV  P      LHC   +    + L   T Y LYLE+LG  +PLL   R+  ++ E+ I+DP +N      PL+     E + S+                  T N GR   S     DS+F   S+F                      L    G + N  DS    L+EL   D LPE   L ++ SN+  TKF++      +P  L ++ +  ++
Sbjct:  172 TITCGIWTPDDQQVYFGTTQGQVIVM--------DVHGAMVSQVQLSNDVPITSMAWSCEKFKMEE--GE-----------------------EAEPGVTNAAKRS------------FVLAVSFQNGYIYLLKSFDDVSPAHINTCLNGALGMVMEWSNSRELLAVAGTLRTGVDGTKTEDMGTPSCYTNLVKFYTETGTCLYQAHIPCSSATVSAITWGHNDKRLFIATGTQVHIAWVSRRVASLQLLCRLEIQASVG-SESLLPLLPLPSRIKSLIGNLFAQTIRCCVPDLKSLRDFVSRPPLCSTRLHCTMIRHDDDSNLSSGTCYTLYLEYLGGLVPLLKGKRTSKIRPEFVIFDPQVND----SPLYFQYSAEAKSSS-----------GSSQSTTTGNSGRTDSS-----DSDFEERSRFGSPRTPRKKRVRPKRRHQAGDRLSASGGGVSNDPDS----LDELAYVDTLPEEVKLVEVTSNIWGTKFKIHGLAKTVPANLGQVTYKTSL 618          

HSP 2 Score: 106.301 bits (264), Expect = 8.310e-23
Identity = 50/132 (37.88%), Postives = 76/132 (57.58%), Query Frame = 3
Query:  219 GWLACGNKTGSVSITKTMIESDDSTESIPYELPARTNYNLRNSKVPINIVEWNPHHKKLATCSNTGSILVYHNVNNQWSIDLVNERAHAVEVFKWSRSTNQVMIGYTDGLLIIGSVSGHRLWHRMMDMHSAL 614
            GWLA GN  G V +T T      +      + P RTNYNLR  +  + +V+WN  ++KLA+C ++G I V+     +WSI+L+N+R   V  F WS      +I Y DG +++GSV+G R W  M+++ S +
Sbjct:   47 GWLATGNVRGIVGVTFTTSHCRKN-----MDYPLRTNYNLRGHRSDVILVKWNEPYQKLASCDSSGIIFVWIKYEGRWSIELINDRNTPVTHFSWSHDGRMALICYQDGFVLVGSVAGQRYWSSMLNLESTI 173          

HSP 3 Score: 57.3806 bits (137), Expect = 7.755e-8
Identity = 28/86 (32.56%), Postives = 47/86 (54.65%), Query Frame = 3
Query: 2862 PIPSFRTFFVSNE-----------QKDSKHDRYLHSSKRIILKYDNDQILDFSRLENDKYSLEFKYPFSPLQAFAVAIANISRLFR 3086
            P P  R F + N+           Q D        S+K   +++   Q++ F R++ + Y+L+F+YPFS LQAFAVA+AN+++  +
Sbjct: 1347 PAPVRREFVMHNKAPMWNEMSQVYQLDFGGRVTQESAKNFQIEFRGKQVMQFGRIDGNAYTLDFQYPFSALQAFAVALANVTQRLK 1432          
BLAST of TUB like protein 4 vs. Ensembl Fly
Match: Tusp (gene:FBgn0039530 transcript:FBtr0335245)

HSP 1 Score: 127.487 bits (319), Expect = 2.852e-29
Identity = 139/518 (26.83%), Postives = 218/518 (42.08%), Query Frame = 3
Query: 1008 TLTCGTWSPNDATIIVGTSIGSLLSFPYAWEXXXXXXXXXXXXXXXXND-SILQVEWSCPPFRRNENGGESITNQDQKYLINSNMKYPHNSFPDSEIPLNDSAASSDLENQLFDNSENFLLAVAFSSGYIYLLRHLEDVDPIKHQT---GLSRLCINWNSTGTILAVGGFVRSA----------NLECQCS-VKLLSTSAEIIYFVPVPSMNKPIMSVTWGHEDTRLFVSASICVHTFRVSSSFSSLTQRCCHIIRNSLKFRQESVDKLCLPSYLHKKIHNSFRKTIQGSIPLPFRLKEFVLLPK--QDVLHCIFQKVSTGNGL---TKYILYLEFLGASIPLLTASRSRSVKYEYHIYDPSLNQVVDSDPLFPNEEMEVEHSNCTSQCVWQRKFKHQNRRKTQNCGRNSISVLRDTDSNF---SQF----------------------LRQESGKIDNCIDSKRSRLNELMTNDDLPENCL-LAKLKSNVTLTKFELTSCQICLPRILSRINFGVNI 2423
            T+TCG W+P+D  +  GT+ G ++                  ++   ND  I  + WSC  F+  E  GE                       ++E  + ++A  S            F+LAV+F +GYIYLL+  +DV P    T   G   + + W+++  +LAV G +R+              C  + VK  + +   +Y   +P  +  + ++TWGH D RLF++    VH   VS   +SL   C   I+ S+   +  +  L LPS +   I N F +TI+  +P    L++FV  P      LHC   +    + L   T Y LYLE+LG  +PLL   R+  ++ E+ I+DP   QV DS PL+     E + S+                  T N GR   S     DS+F   S+F                      L    G + N  DS    L+EL   D LPE  + L ++ SN+  TKF++      +P  L ++ +  ++
Sbjct:  172 TITCGIWTPDDQQVYFGTTQGQVIVM--------DVHGAMVSQVQLSNDVPITSMAWSCEKFKMEE--GE-----------------------EAEPGVTNAAKRS------------FVLAVSFQNGYIYLLKSFDDVSPAHINTCLNGALGMVMEWSNSRELLAVAGTLRTGVDGTKTEDMGTPSCYTNLVKFYTETGTCLYQAHIPCSSATVSAITWGHNDKRLFIATGTQVHIAWVSRRVASLQLLCRLEIQASVG-SESLLPLLPLPSRIKSLIGNLFAQTIRCCVPDLKSLRDFVSRPPLCSTRLHCTMIRHDDDSNLSSGTCYTLYLEYLGGLVPLLKGKRTSKIRPEFVIFDP---QVNDS-PLYFQYSAEAKSSS-----------GSSQSTTTGNSGRTDSS-----DSDFEERSRFGSPRTPRKKRVRPKRRHQAGDRLSASGGGVSNDPDS----LDELAYVDTLPEQEVKLVEVTSNIWGTKFKIHGLAKTVPANLGQVTYKTSL 619          

HSP 2 Score: 106.301 bits (264), Expect = 8.384e-23
Identity = 50/132 (37.88%), Postives = 76/132 (57.58%), Query Frame = 3
Query:  219 GWLACGNKTGSVSITKTMIESDDSTESIPYELPARTNYNLRNSKVPINIVEWNPHHKKLATCSNTGSILVYHNVNNQWSIDLVNERAHAVEVFKWSRSTNQVMIGYTDGLLIIGSVSGHRLWHRMMDMHSAL 614
            GWLA GN  G V +T T      +      + P RTNYNLR  +  + +V+WN  ++KLA+C ++G I V+     +WSI+L+N+R   V  F WS      +I Y DG +++GSV+G R W  M+++ S +
Sbjct:   47 GWLATGNVRGIVGVTFTTSHCRKN-----MDYPLRTNYNLRGHRSDVILVKWNEPYQKLASCDSSGIIFVWIKYEGRWSIELINDRNTPVTHFSWSHDGRMALICYQDGFVLVGSVAGQRYWSSMLNLESTI 173          

HSP 3 Score: 57.3806 bits (137), Expect = 7.562e-8
Identity = 28/86 (32.56%), Postives = 47/86 (54.65%), Query Frame = 3
Query: 2862 PIPSFRTFFVSNE-----------QKDSKHDRYLHSSKRIILKYDNDQILDFSRLENDKYSLEFKYPFSPLQAFAVAIANISRLFR 3086
            P P  R F + N+           Q D        S+K   +++   Q++ F R++ + Y+L+F+YPFS LQAFAVA+AN+++  +
Sbjct: 1348 PAPVRREFVMHNKAPMWNEMSQVYQLDFGGRVTQESAKNFQIEFRGKQVMQFGRIDGNAYTLDFQYPFSALQAFAVALANVTQRLK 1433          
BLAST of TUB like protein 4 vs. Ensembl Fly
Match: Oseg4 (gene:FBgn0035264 transcript:FBtr0072797)

HSP 1 Score: 53.1434 bits (126), Expect = 1.527e-6
Identity = 26/91 (28.57%), Postives = 47/91 (51.65%), Query Frame = 3
Query:  327 NYNLRNSKVPINIVEWNPHHKKLATCSNTGSILVYHNVNNQWSIDLVNERAHA-VEVFKWSRSTNQVMIGYTDGLLIIGSVSGHRLWHRMM 596
            N  L   K  + +V WN   +KL +    G I+V+      W  ++ N+R  + V    W+   +++ I Y DG +I+GSV G+R++ + +
Sbjct:   65 NQTLDGHKESVRVVTWNDAQQKLTSSDTDGVIMVWMLYKGSWYEEMTNDRKKSTVASMSWTSDGSRICIVYEDGAIIVGSVDGNRIFGKEL 155          
BLAST of TUB like protein 4 vs. Ensembl Zebrafish
Match: tulp4b (TUB like protein 4b [Source:ZFIN;Acc:ZDB-GENE-130530-708])

HSP 1 Score: 140.198 bits (352), Expect = 4.847e-33
Identity = 128/510 (25.10%), Postives = 219/510 (42.94%), Query Frame = 3
Query: 1011 LTCGTWSPNDATIIVGTSIGSLLSFPYAWEXXXXXXXXXXXXXXXXNDSILQVEWSCPPFRRNENGGESITNQDQKYLINSNMKYPHNSFPDSEIPLNDSAASSDLENQLFDNSENFLLAVAFSSGYIYLLRHLEDVDPIKHQTGLSRLCINWNSTGTILAVGGFVRSA----NLECQCSVKLLSTSAEIIYFVPVPSMNKPIMSVTWGHEDTRLFVSASICVHTFRVSSSFSSLTQRCCHIIRNSLKFRQESVDKLCLPSYLHKKIHNSFRKTIQGSIPLPFRLKEFVLLPKQ--DVLHCIFQKVSTG--NGLTKYILYLEFLGASIPLLTASRSRSVKYEYHIYDPSLNQVVDSDPLFPNEEMEVEHSNC--------TSQCVWQRKFKHQNRRKTQNCGRNSISVLRDTDSNFSQFLRQESGKIDNC--IDSKRSRLNELMTNDDLP-----ENCLLAKLKSNVTLTKFELTSCQICLPRILSRINFGVN-IKLNPSNGVSVHLVEI 2468
            +TCG W+P+D  ++ GT+ G ++                   LL  +D I+ + W+CP F   ++      + DQ      N+K                                 LL V+F SG I L+ + +D+ P   ++GL  + + W S G +LAV G  R      +L     VK  +   E IY +  P+  +PI ++ WGH D+RLF++    ++  RV    +SL   C   I ++LK  +  V KL +PS L   +  +F  TI+  IP P  +++FV  P    + LHC  ++       G   Y LYLE LG  +P+L   R   ++ E+ I DP ++   D   +        +  NC        + + V ++  K     ++    R S  +  + +S  S  L Q S ++     +  K    +  +T  + P     E+  LA++ SN+  TKF++      LP  L  + +  + + L P   ++++L E+
Sbjct:  183 ITCGIWTPDDQQVLFGTADGQVIVM-------DCHGRMLAHVLLHESDGIVSMSWNCPNFLVEDSTESDTDSDDQTPAHVQNLKP--------------------------------LLTVSFISGDISLMNNYDDLSPNIIRSGLKDVEVQWCSQGDLLAVAGMERHGLPTDSLMRNALVKFYNVQGEHIYTLETPA-QRPITTICWGHRDSRLFLACGPALYVVRVEHRVASLQLLCQQGIASALK-EERDVGKLNMPSLLCSYVTTAFIPTIKPPIPDPNNIRDFVSYPTAGNERLHCTMKRSEENPEAGGPCYTLYLEHLGGLVPILKGRRISKLRPEFVIMDPKMDGKADEVCVNGMISYMTDSCNCSDSSDIELSDEWVGRKSPKLSRGNRSPKLPRYSKHIRINMESRKSPKLAQTSQEMSRSPRLPKKPPLRSPSLTRREFPVDGFSEHNYLAQVTSNIWGTKFKIVGLASFLPANLGAVIYKTSLLHLQPRQ-MTIYLPEV 650          

HSP 2 Score: 103.99 bits (258), Expect = 7.095e-22
Identity = 52/134 (38.81%), Postives = 71/134 (52.99%), Query Frame = 3
Query:  219 GWLACGNKTGSVSITKTM--IESDDSTESIPYELPARTNYNLRNSKVPINIVEWNPHHKKLATCSNTGSILVYHNVNNQWSIDLVNERAHAVEVFKWSRSTNQVMIGYTDGLLIIGSVSGHRLWHRMMDMHSAL 614
            GWLA GN  G V +T T      D +T       P R N+NLR     + +V WN   +KLATC   G I V+     +WS++LVN+R   V  F WS    Q +I Y DG +++GSVSG R W   +++ S +
Sbjct:   57 GWLATGNARGVVGVTFTSSHCRRDRNT-------PQRINFNLRGHNSEVVLVRWNEPFQKLATCDMEGGIFVWIQYEGRWSVELVNDRGAQVSDFTWSHDGTQALIAYRDGFVLVGSVSGQRHWSSEINLESQI 183          

HSP 3 Score: 55.8398 bits (133), Expect = 3.647e-7
Identity = 21/52 (40.38%), Postives = 37/52 (71.15%), Query Frame = 3
Query: 2931 HSSKRIILKYDNDQILDFSRLENDKYSLEFKYPFSPLQAFAVAIANISRLFR 3086
             S+K   ++ D  Q++ F R++ + Y L+F+YPFS +QAFAVA+AN+++  +
Sbjct: 1565 ESAKNFQIELDGRQVMQFGRIDGNAYILDFQYPFSAVQAFAVALANVTQRLK 1616          
BLAST of TUB like protein 4 vs. Ensembl Zebrafish
Match: tulp4b (TUB like protein 4b [Source:ZFIN;Acc:ZDB-GENE-130530-708])

HSP 1 Score: 138.272 bits (347), Expect = 2.144e-32
Identity = 128/504 (25.40%), Postives = 219/504 (43.45%), Query Frame = 3
Query: 1011 LTCGTWSPNDATIIVGTSIGSLLSFPYAWEXXXXXXXXXXXXXXXXNDSILQVEWSCPPFRRNENGGESITNQDQKYLINSNMKYPHNSFPDSEIPLNDSAASSDLENQLFDNSENFLLAVAFSSGYIYLLRHLEDVDPIKHQTGLSRLCINWNSTGTILAVGGFVRSA----NLECQCSVKLLSTSAEIIYFVPVPSMNKPIMSVTWGHEDTRLFVSASICVHTFRVSSSFSSLTQRCCHIIRNSLKFRQESVDKLCLPSYLHKKIHNSFRKTIQGSIPLPFRLKEFVLLPKQ--DVLHCIFQKVSTG--NGLTKYILYLEFLGASIPLLTASRSRSVKYEYHIYDPSLNQVVDSDPLFPNEEMEVEHSNCTSQCVWQRKFKHQNRRKTQ-NCGRNSISVLR-DTDSNFSQFLRQESGKIDNC--IDSKRSRLNELMTNDDLP-----ENCLLAKLKSNVTLTKFELTSCQICLPRILSRINFGVN-IKLNPSNGVSVHLVEI 2468
            +TCG W+P+D  ++ GT+ G ++                   LL  +D I+ + W+CP F   ++      + DQ      N+K                                 LL V+F SG I L+ + +D+ P   ++GL  + + W S G +LAV G  R      +L     VK  +   E IY +  P+  +PI ++ WGH D+RLF++    ++  RV    +SL   C   I ++LK  +  V KL +PS L   +  +F  TI+  IP P  +++FV  P    + LHC  ++       G   Y LYLE LG  +P+L   R   ++ E+ I DP ++   D   +        +  NC+     +   +   R+  + + G  S  + R + +S  S  L Q S ++     +  K    +  +T  + P     E+  LA++ SN+  TKF++      LP  L  + +  + + L P   ++++L E+
Sbjct:  183 ITCGIWTPDDQQVLFGTADGQVIVM-------DCHGRMLAHVLLHESDGIVSMSWNCPNFLVEDSTESDTDSDDQTPAHVQNLKP--------------------------------LLTVSFISGDISLMNNYDDLSPNIIRSGLKDVEVQWCSQGDLLAVAGMERHGLPTDSLMRNALVKFYNVQGEHIYTLETPA-QRPITTICWGHRDSRLFLACGPALYVVRVEHRVASLQLLCQQGIASALK-EERDVGKLNMPSLLCSYVTTAFIPTIKPPIPDPNNIRDFVSYPTAGNERLHCTMKRSEENPEAGGPCYTLYLEHLGGLVPILKGRRISKLRPEFVIMDPKMDGKADEVCVNGMISYMTDSCNCSDSSDIELSDEWVGRKSPKLSRGNRSPKLPRINMESRKSPKLAQTSQEMSRSPRLPKKPPLRSPSLTRREFPVDGFSEHNYLAQVTSNIWGTKFKIVGLASFLPANLGAVIYKTSLLHLQPRQ-MTIYLPEV 644          

HSP 2 Score: 103.605 bits (257), Expect = 8.153e-22
Identity = 52/134 (38.81%), Postives = 71/134 (52.99%), Query Frame = 3
Query:  219 GWLACGNKTGSVSITKTM--IESDDSTESIPYELPARTNYNLRNSKVPINIVEWNPHHKKLATCSNTGSILVYHNVNNQWSIDLVNERAHAVEVFKWSRSTNQVMIGYTDGLLIIGSVSGHRLWHRMMDMHSAL 614
            GWLA GN  G V +T T      D +T       P R N+NLR     + +V WN   +KLATC   G I V+     +WS++LVN+R   V  F WS    Q +I Y DG +++GSVSG R W   +++ S +
Sbjct:   57 GWLATGNARGVVGVTFTSSHCRRDRNT-------PQRINFNLRGHNSEVVLVRWNEPFQKLATCDMEGGIFVWIQYEGRWSVELVNDRGAQVSDFTWSHDGTQALIAYRDGFVLVGSVSGQRHWSSEINLESQI 183          

HSP 3 Score: 55.4546 bits (132), Expect = 3.878e-7
Identity = 21/52 (40.38%), Postives = 37/52 (71.15%), Query Frame = 3
Query: 2931 HSSKRIILKYDNDQILDFSRLENDKYSLEFKYPFSPLQAFAVAIANISRLFR 3086
             S+K   ++ D  Q++ F R++ + Y L+F+YPFS +QAFAVA+AN+++  +
Sbjct: 1791 ESAKNFQIELDGRQVMQFGRIDGNAYILDFQYPFSAVQAFAVALANVTQRLK 1842          
BLAST of TUB like protein 4 vs. Ensembl Zebrafish
Match: tulp4a (TUB like protein 4a [Source:ZFIN;Acc:ZDB-GENE-060503-215])

HSP 1 Score: 127.487 bits (319), Expect = 3.474e-29
Identity = 109/440 (24.77%), Postives = 201/440 (45.68%), Query Frame = 3
Query: 1362 LLAVAFSSGYIYLLRHLEDVDPIKHQTGLSRLCINWNSTGTILAVGGFVRSANLECQCS-----VKLLSTSAEIIYFVPVPSMNKPIMSVTWGHEDTRLFVSASICVHTFRVSSSFSSLTQRCCHIIRNSLKFRQESVDKLCLPSYLHKKIHNSFRKTIQGSIPLPFRLKEFVLLPKQ--DVLHCIFQKVSTGN--GLTKYILYLEFLGASIPLLTASRSRSVKYEYHIYDPSLNQVVDSDPLFPNEEME--VEHSNCTSQCVWQRKFKHQNRRKTQ-NCGRNSISVLRDTDSNFSQFLRQESGKIDNCIDSKRSRLNELMTN-----------------------DDLPENCLLAKLKSNVTLTKFELTSCQICLPRILSRINFGVNIKLNPSNGVSVHLVEISNSTGQKMTNYYRDI-CLTVQGLSVSCYAENAFQSD 2573
            LL V F+SG I L+ + +D+ P+  +TGL  + + W+S G +LAV G  R    +   +     VK  +   E IY +  P+  +PI ++ WGH D+R+F++    ++  RV    SSL   C   I +++   ++ + KL +PS L   + ++F  TI+  IP P  +++FV  P    + LHC  ++       G   Y LYLE+LG  +P+L   R   ++ E+ I DP  +    +D ++ N  +   ++  NC+     +       ++  + + G  S  + RD    F+  +      ID     K SR  + ++                        DD+ ++  LA++ SN+  TKF++      LP  L  + +  ++         +HL        ++MT Y  ++  ++V+ +++  ++ N F  D
Sbjct:  248 LLTVCFTSGDISLMSNYDDLSPLLIRTGLKDVVVQWSSQGDLLAVAGMERLVTSDPTQATRNAIVKFYNVRGEHIYSLETPA-QRPISTLCWGHRDSRVFLACGPALYVLRVEHRVSSLQLLCQQAIASAV-HEEKDLAKLSMPSRLSTYVSSAFAPTIKPPIPDPNNMRDFVSYPTSGNERLHCTMKRTEDNPEVGGPCYTLYLEYLGGLVPILKGRRISKLRPEFVIMDPKTDG--KTDEVYGNSFIPSMIDSCNCSDSSDIELNDDWVGKKSPKISRGSKSPKLPRDLVCPFTNRI-----NIDPRKSPKLSRTTQEISRSPRLPIRKPSIGSPSLTRREFPLDDITQHNYLAQVTSNIWGTKFKIVGLAAFLPTNLGAVIYKTSL---------LHLQP------RQMTIYLPEVRKISVEYINLPMFSPNVFSED 663          

HSP 2 Score: 103.219 bits (256), Expect = 9.602e-22
Identity = 52/134 (38.81%), Postives = 71/134 (52.99%), Query Frame = 3
Query:  219 GWLACGNKTGSVSITKTM--IESDDSTESIPYELPARTNYNLRNSKVPINIVEWNPHHKKLATCSNTGSILVYHNVNNQWSIDLVNERAHAVEVFKWSRSTNQVMIGYTDGLLIIGSVSGHRLWHRMMDMHSAL 614
            GWLA GN  G V +T T      D +T       P R N+NLR     + +V WN   +KLATC   G I V+     +WS++LVN+R   V  F WS    Q +I Y DG +++GSVSG R W   +++ S +
Sbjct:   44 GWLATGNGRGVVGVTFTSSHCRRDRNT-------PQRINFNLRGHNSEVVLVRWNEPFQKLATCDTDGGIFVWIQYEGRWSVELVNDRGAQVSDFTWSHDGTQALISYRDGFVLVGSVSGQRHWSSEINLESQI 170          

HSP 3 Score: 53.9138 bits (128), Expect = 1.355e-6
Identity = 20/52 (38.46%), Postives = 37/52 (71.15%), Query Frame = 3
Query: 2931 HSSKRIILKYDNDQILDFSRLENDKYSLEFKYPFSPLQAFAVAIANISRLFR 3086
             S+K   ++ +  Q++ F R++ + Y L+F+YPFS +QAFAVA+AN+++  +
Sbjct: 1588 ESAKNFQIELEGRQVMQFGRIDGNAYILDFQYPFSAVQAFAVALANVTQRLK 1639          
BLAST of TUB like protein 4 vs. Ensembl Zebrafish
Match: tulp4a (TUB like protein 4a [Source:ZFIN;Acc:ZDB-GENE-060503-215])

HSP 1 Score: 127.487 bits (319), Expect = 4.496e-29
Identity = 109/440 (24.77%), Postives = 201/440 (45.68%), Query Frame = 3
Query: 1362 LLAVAFSSGYIYLLRHLEDVDPIKHQTGLSRLCINWNSTGTILAVGGFVRSANLECQCS-----VKLLSTSAEIIYFVPVPSMNKPIMSVTWGHEDTRLFVSASICVHTFRVSSSFSSLTQRCCHIIRNSLKFRQESVDKLCLPSYLHKKIHNSFRKTIQGSIPLPFRLKEFVLLPKQ--DVLHCIFQKVSTGN--GLTKYILYLEFLGASIPLLTASRSRSVKYEYHIYDPSLNQVVDSDPLFPNEEME--VEHSNCTSQCVWQRKFKHQNRRKTQ-NCGRNSISVLRDTDSNFSQFLRQESGKIDNCIDSKRSRLNELMTN-----------------------DDLPENCLLAKLKSNVTLTKFELTSCQICLPRILSRINFGVNIKLNPSNGVSVHLVEISNSTGQKMTNYYRDI-CLTVQGLSVSCYAENAFQSD 2573
            LL V F+SG I L+ + +D+ P+  +TGL  + + W+S G +LAV G  R    +   +     VK  +   E IY +  P+  +PI ++ WGH D+R+F++    ++  RV    SSL   C   I +++   ++ + KL +PS L   + ++F  TI+  IP P  +++FV  P    + LHC  ++       G   Y LYLE+LG  +P+L   R   ++ E+ I DP  +    +D ++ N  +   ++  NC+     +       ++  + + G  S  + RD    F+  +      ID     K SR  + ++                        DD+ ++  LA++ SN+  TKF++      LP  L  + +  ++         +HL        ++MT Y  ++  ++V+ +++  ++ N F  D
Sbjct:  248 LLTVCFTSGDISLMSNYDDLSPLLIRTGLKDVVVQWSSQGDLLAVAGMERLVTSDPTQATRNAIVKFYNVRGEHIYSLETPA-QRPISTLCWGHRDSRVFLACGPALYVLRVEHRVSSLQLLCQQAIASAV-HEEKDLAKLSMPSRLSTYVSSAFAPTIKPPIPDPNNMRDFVSYPTSGNERLHCTMKRTEDNPEVGGPCYTLYLEYLGGLVPILKGRRISKLRPEFVIMDPKTDG--KTDEVYGNSFIPSMIDSCNCSDSSDIELNDDWVGKKSPKISRGSKSPKLPRDLVCPFTNRI-----NIDPRKSPKLSRTTQEISRSPRLPIRKPSIGSPSLTRREFPLDDITQHNYLAQVTSNIWGTKFKIVGLAAFLPTNLGAVIYKTSL---------LHLQP------RQMTIYLPEVRKISVEYINLPMFSPNVFSED 663          

HSP 2 Score: 103.219 bits (256), Expect = 1.058e-21
Identity = 52/134 (38.81%), Postives = 71/134 (52.99%), Query Frame = 3
Query:  219 GWLACGNKTGSVSITKTM--IESDDSTESIPYELPARTNYNLRNSKVPINIVEWNPHHKKLATCSNTGSILVYHNVNNQWSIDLVNERAHAVEVFKWSRSTNQVMIGYTDGLLIIGSVSGHRLWHRMMDMHSAL 614
            GWLA GN  G V +T T      D +T       P R N+NLR     + +V WN   +KLATC   G I V+     +WS++LVN+R   V  F WS    Q +I Y DG +++GSVSG R W   +++ S +
Sbjct:   44 GWLATGNGRGVVGVTFTSSHCRRDRNT-------PQRINFNLRGHNSEVVLVRWNEPFQKLATCDTDGGIFVWIQYEGRWSVELVNDRGAQVSDFTWSHDGTQALISYRDGFVLVGSVSGQRHWSSEINLESQI 170          

HSP 3 Score: 53.9138 bits (128), Expect = 1.402e-6
Identity = 20/52 (38.46%), Postives = 37/52 (71.15%), Query Frame = 3
Query: 2931 HSSKRIILKYDNDQILDFSRLENDKYSLEFKYPFSPLQAFAVAIANISRLFR 3086
             S+K   ++ +  Q++ F R++ + Y L+F+YPFS +QAFAVA+AN+++  +
Sbjct: 1685 ESAKNFQIELEGRQVMQFGRIDGNAYILDFQYPFSAVQAFAVALANVTQRLK 1736          
BLAST of TUB like protein 4 vs. Ensembl Zebrafish
Match: wdr35 (WD repeat domain 35 [Source:ZFIN;Acc:ZDB-GENE-060810-148])

HSP 1 Score: 68.1662 bits (165), Expect = 6.004e-11
Identity = 38/137 (27.74%), Postives = 66/137 (48.18%), Query Frame = 3
Query:  186 SWMQDHTHSNIGWLACGNKTGSVSITKTMIESDDST-ESIPYELPARTNYNLRNSKVPINIVEWNPHHKKLATCSNTGSILVYHNVNNQWSIDLVNERAHAV-EVFKWSRSTNQVMIGYTDGLLIIGSVSGHRLWHR 590
            SW +D      G++ACG + G + + K    +DD+  + +        N  L      + +V WN  ++KL T    G I+V+      W  +++N R  +V     W+    ++ I Y DG +I+GSV G+R+W +
Sbjct:   21 SWNKDQ-----GFIACGGEDGLLKVLKLETYTDDAKLKGLAAPSNLSMNQTLEGHSGAVQVVTWNEQYQKLTTSDQNGLIIVWMLYKGSWYEEMINNRNKSVVRSMSWNADGLKICIVYEDGAVIVGSVDGNRIWGK 152          
BLAST of TUB like protein 4 vs. Ensembl Xenopus
Match: tulp4 (B-cell receptor CD22-like [Source:Xenbase;Acc:XB-GENE-5887253])

HSP 1 Score: 127.487 bits (319), Expect = 5.178e-29
Identity = 123/512 (24.02%), Postives = 220/512 (42.97%), Query Frame = 3
Query: 1011 LTCGTWSPNDATIIVGTSIGSLLSFPYAWEXXXXXXXXXXXXXXXXNDSILQVEWSCPPFRRNENGGESITNQDQKYLINSNMKYPHNSFPDSEIPLNDSAASSDLENQLFDNSENFLLAVAFSSGYIYLLRHLEDVDPIKHQTGLSRLCINWNSTGTILAVGGFVRS--------ANLECQCSVKLLSTSAEIIYFVPVPSMNKPIMSVTWGHEDTRLFVSASICVHTFRVSSSFSSLTQRCCHIIRNSLKFRQESVDKLCLPSYLHKKIHNSFRKTIQGSIPLPFRLKEFVLLPKQ--DVLHCIFQKVSTGN--GLTKYILYLEFLGASIPLLTASRSRSVKYEYHIYDPSLNQVVDSDPLFPNEEME--VEHSNCTSQC------VWQRKFKHQNRRKTQNCGRNSISVLRDTDSNFSQFLRQESGKIDNCIDSKRSRLNELMTN-----DDLPENCLLAKLKSNVTLTKFELTSCQICLPRILSRINFGVN-IKLNPSNGVSVHLVEI 2468
            +TCG W+P+D  ++ GT+ G ++                   LL  +D I+ + W+ P F   ++      + D     +    YP        IP+ +                  LL V F+SG I L+ + +D+ P   ++GL  + + W + G +LAV G  +          N      +K  +   E IY +  P + +PI S+ WGH D+RLF+++   ++  RV    +SL   C   I  SL   ++ + KL LP  L   +  +F  TI+  IP P  +++FV  P    + LHC  ++       G   Y LYLE+LG  +P+L   R   ++ E+ I DP  +    +D ++ N  +   ++  NC+          W  K   +  R +++     I++         +   QE  +       K S  +  +T      +D+ ++  LA++ SN+  TKF++      LP  L  + +  + + L P   ++++L E+
Sbjct:  170 ITCGIWTPDDQQVLFGTADGQVIVM-------DCHGRMLAHVLLHESDGIVNMSWNYPSFLVEDSSESDTDSDDYSPPQDGPAAYP--------IPVENIKP---------------LLTVGFTSGDIRLMNNYDDLSPTIVRSGLKDVVVQWCTQGDLLAVAGMEKQNLIPEHVNGNSLKSALIKFYNVRGEHIYTLETP-VQRPITSICWGHRDSRLFLASGPALYVVRVEHRVASLQLLCQQTIATSLSDDKD-ICKLNLPPRLCSYLTTAFIPTIKPPIPDPNNMRDFVSYPTSGNERLHCTMKRTEDDPEVGGPCYTLYLEYLGGLVPVLKGRRISKLRPEFVIMDPKTD--AKTDEIYGNGLISSVIDSCNCSDSSDIELSDDWAAKKSPKISRASKSPKLPRINIEARKSPKLPR-AAQEITRSPRLPIRKPSIGSPSLTRRECPLEDITQHNYLAQVTSNIWGTKFKIVGLAAFLPANLGAVIYKTSLLHLQPRQ-MTIYLPEV 645          

HSP 2 Score: 106.686 bits (265), Expect = 1.428e-22
Identity = 53/134 (39.55%), Postives = 73/134 (54.48%), Query Frame = 3
Query:  219 GWLACGNKTGSVSITKTM--IESDDSTESIPYELPARTNYNLRNSKVPINIVEWNPHHKKLATCSNTGSILVYHNVNNQWSIDLVNERAHAVEVFKWSRSTNQVMIGYTDGLLIIGSVSGHRLWHRMMDMHSAL 614
            GWLA GN  G V +T T+     D +T       P RTN+NLR     + +V WN   +KLATC   G I V+     +WS++LVN+R   V  F WS    Q +I Y DG +++GSVSG R W   +++ S +
Sbjct:   44 GWLATGNGRGVVGVTFTLSHCRRDRNT-------PQRTNFNLRGHNSEVVLVRWNEPFQKLATCDADGGIFVWIQYEGRWSVELVNDRGAQVSDFTWSHDGTQALISYRDGFVLVGSVSGQRHWSSEINLESQI 170          

HSP 3 Score: 53.9138 bits (128), Expect = 2.018e-6
Identity = 20/52 (38.46%), Postives = 37/52 (71.15%), Query Frame = 3
Query: 2931 HSSKRIILKYDNDQILDFSRLENDKYSLEFKYPFSPLQAFAVAIANISRLFR 3086
             S+K   ++ +  Q++ F R++ + Y L+F+YPFS +QAFAVA+AN+++  +
Sbjct: 1517 ESAKNFQIELEGRQVMQFGRIDGNAYILDFQYPFSAVQAFAVALANVTQRLK 1568          
BLAST of TUB like protein 4 vs. Ensembl Xenopus
Match: tulp4 (B-cell receptor CD22-like [Source:Xenbase;Acc:XB-GENE-5887253])

HSP 1 Score: 126.716 bits (317), Expect = 1.047e-28
Identity = 96/359 (26.74%), Postives = 157/359 (43.73%), Query Frame = 3
Query: 1011 LTCGTWSPNDATIIVGTSIGSLLSFPYAWEXXXXXXXXXXXXXXXXNDSILQVEWSCPPFRRNENGGESITNQDQKYLINSNMKYPHNSFPDSEIPLNDSAASSDLENQLFDNSENFLLAVAFSSGYIYLLRHLEDVDPIKHQTGLSRLCINWNSTGTILAVGGFVRS--------ANLECQCSVKLLSTSAEIIYFVPVPSMNKPIMSVTWGHEDTRLFVSASICVHTFRVSSSFSSLTQRCCHIIRNSLKFRQESVDKLCLPSYLHKKIHNSFRKTIQGSIPLPFRLKEFVLLPKQ--DVLHCIFQKVSTGN--GLTKYILYLEFLGASIPLLTASRSRSVKYEYHIYDPSLNQVVD 2051
            +TCG W+P+D  ++ GT+ G ++                   LL  +D I+ + W+ P F   ++      + D     +    YP        IP+ +                  LL V F+SG I L+ + +D+ P   ++GL  + + W + G +LAV G  +          N      +K  +   E IY +  P + +PI S+ WGH D+RLF+++   ++  RV    +SL   C   I  SL    + + KL LP  L   +  +F  TI+  IP P  +++FV  P    + LHC  ++       G   Y LYLE+LG  +P+L   R   ++ E+ I DP  +   D
Sbjct:  170 ITCGIWTPDDQQVLFGTADGQVIVM-------DCHGRMLAHVLLHESDGIVNMSWNYPSFLVEDSSESDTDSDDYSPPQDGPAAYP--------IPVENIKP---------------LLTVGFTSGDIRLMNNYDDLSPTIVRSGLKDVVVQWCTQGDLLAVAGMEKQNLIPEHVNGNSLKSALIKFYNVRGEHIYTLETP-VQRPITSICWGHRDSRLFLASGPALYVVRVEHRVASLQLLCQQTIATSLS-DDKDICKLNLPPRLCSYLTTAFIPTIKPPIPDPNNMRDFVSYPTSGNERLHCTMKRTEDDPEVGGPCYTLYLEYLGGLVPVLKGRRISKLRPEFVIMDPKTDAKTD 496          

HSP 2 Score: 107.071 bits (266), Expect = 1.135e-22
Identity = 53/134 (39.55%), Postives = 73/134 (54.48%), Query Frame = 3
Query:  219 GWLACGNKTGSVSITKTM--IESDDSTESIPYELPARTNYNLRNSKVPINIVEWNPHHKKLATCSNTGSILVYHNVNNQWSIDLVNERAHAVEVFKWSRSTNQVMIGYTDGLLIIGSVSGHRLWHRMMDMHSAL 614
            GWLA GN  G V +T T+     D +T       P RTN+NLR     + +V WN   +KLATC   G I V+     +WS++LVN+R   V  F WS    Q +I Y DG +++GSVSG R W   +++ S +
Sbjct:   44 GWLATGNGRGVVGVTFTLSHCRRDRNT-------PQRTNFNLRGHNSEVVLVRWNEPFQKLATCDADGGIFVWIQYEGRWSVELVNDRGAQVSDFTWSHDGTQALISYRDGFVLVGSVSGQRHWSSEINLESQI 170          

HSP 3 Score: 53.5286 bits (127), Expect = 2.099e-6
Identity = 20/52 (38.46%), Postives = 37/52 (71.15%), Query Frame = 3
Query: 2931 HSSKRIILKYDNDQILDFSRLENDKYSLEFKYPFSPLQAFAVAIANISRLFR 3086
             S+K   ++ +  Q++ F R++ + Y L+F+YPFS +QAFAVA+AN+++  +
Sbjct: 1485 ESAKNFQIELEGRQVMQFGRIDGNAYILDFQYPFSAVQAFAVALANVTQRLK 1536          
BLAST of TUB like protein 4 vs. Ensembl Xenopus
Match: tulp4 (B-cell receptor CD22-like [Source:Xenbase;Acc:XB-GENE-5887253])

HSP 1 Score: 126.331 bits (316), Expect = 1.225e-28
Identity = 96/359 (26.74%), Postives = 159/359 (44.29%), Query Frame = 3
Query: 1011 LTCGTWSPNDATIIVGTSIGSLLSFPYAWEXXXXXXXXXXXXXXXXNDSILQVEWSCPPFRRNENGGESITNQDQKYLINSNMKYPHNSFPDSEIPLNDSAASSDLENQLFDNSENFLLAVAFSSGYIYLLRHLEDVDPIKHQTGLSRLCINWNSTGTILAVGGFVR--------SANLECQCSVKLLSTSAEIIYFVPVPSMNKPIMSVTWGHEDTRLFVSASICVHTFRVSSSFSSLTQRCCHIIRNSLKFRQESVDKLCLPSYLHKKIHNSFRKTIQGSIPLPFRLKEFVLLPKQ--DVLHCIFQKVSTGN--GLTKYILYLEFLGASIPLLTASRSRSVKYEYHIYDPSLNQVVD 2051
            +TCG W+P+D  ++ GT+ G ++                   LL  +D I+ + W+ P F   ++      + D     +    YP        IP+ +                  LL V F+SG I L+ + +D+ P   ++GL  + + W + G +LAV G  +        + N      +K  +   E IY +  P + +PI S+ WGH D+RLF+++   ++  RV    +SL   C   I  SL   ++ + KL LP  L   +  +F  TI+  IP P  +++FV  P    + LHC  ++       G   Y LYLE+LG  +P+L   R   ++ E+ I DP  +   D
Sbjct:  170 ITCGIWTPDDQQVLFGTADGQVIVM-------DCHGRMLAHVLLHESDGIVNMSWNYPSFLVEDSSESDTDSDDYSPPQDGPAAYP--------IPVENIKP---------------LLTVGFTSGDIRLMNNYDDLSPTIVRSGLKDVVVQWCTQGDLLAVAGMEKQNLIPEHVNGNSLKSALIKFYNVRGEHIYTLETP-VQRPITSICWGHRDSRLFLASGPALYVVRVEHRVASLQLLCQQTIATSLSDDKD-ICKLNLPPRLCSYLTTAFIPTIKPPIPDPNNMRDFVSYPTSGNERLHCTMKRTEDDPEVGGPCYTLYLEYLGGLVPVLKGRRISKLRPEFVIMDPKTDAKTD 496          

HSP 2 Score: 106.301 bits (264), Expect = 1.558e-22
Identity = 53/134 (39.55%), Postives = 73/134 (54.48%), Query Frame = 3
Query:  219 GWLACGNKTGSVSITKTM--IESDDSTESIPYELPARTNYNLRNSKVPINIVEWNPHHKKLATCSNTGSILVYHNVNNQWSIDLVNERAHAVEVFKWSRSTNQVMIGYTDGLLIIGSVSGHRLWHRMMDMHSAL 614
            GWLA GN  G V +T T+     D +T       P RTN+NLR     + +V WN   +KLATC   G I V+     +WS++LVN+R   V  F WS    Q +I Y DG +++GSVSG R W   +++ S +
Sbjct:   44 GWLATGNGRGVVGVTFTLSHCRRDRNT-------PQRTNFNLRGHNSEVVLVRWNEPFQKLATCDADGGIFVWIQYEGRWSVELVNDRGAQVSDFTWSHDGTQALISYRDGFVLVGSVSGQRHWSSEINLESQI 170          

HSP 3 Score: 53.9138 bits (128), Expect = 1.914e-6
Identity = 20/52 (38.46%), Postives = 37/52 (71.15%), Query Frame = 3
Query: 2931 HSSKRIILKYDNDQILDFSRLENDKYSLEFKYPFSPLQAFAVAIANISRLFR 3086
             S+K   ++ +  Q++ F R++ + Y L+F+YPFS +QAFAVA+AN+++  +
Sbjct: 1493 ESAKNFQIELEGRQVMQFGRIDGNAYILDFQYPFSAVQAFAVALANVTQRLK 1544          
BLAST of TUB like protein 4 vs. Ensembl Xenopus
Match: tulp4 (B-cell receptor CD22-like [Source:Xenbase;Acc:XB-GENE-5887253])

HSP 1 Score: 120.553 bits (301), Expect = 6.594e-27
Identity = 102/395 (25.82%), Postives = 181/395 (45.82%), Query Frame = 3
Query: 1362 LLAVAFSSGYIYLLRHLEDVDPIKHQTGLSRLCINWNSTGTILAVGGFVRS--------ANLECQCSVKLLSTSAEIIYFVPVPSMNKPIMSVTWGHEDTRLFVSASICVHTFRVSSSFSSLTQRCCHIIRNSLKFRQESVDKLCLPSYLHKKIHNSFRKTIQGSIPLPFRLKEFVLLPKQ--DVLHCIFQKVSTGN--GLTKYILYLEFLGASIPLLTASRSRSVKYEYHIYDPSLNQVVDSDPLFPNEEME--VEHSNCTSQC------VWQRKFKHQNRRKTQNCGRNSISVLRDTDSNFSQFLRQESGKIDNCIDSKRSRLNELMTN-----DDLPENCLLAKLKSNVTLTKFELTSCQICLPRILSRINFGVN-IKLNPSNGVSVHLVEI 2468
            LL V F+SG I L+ + +D+ P   ++GL  + + W + G +LAV G  +          N      +K  +   E IY +  P + +PI S+ WGH D+RLF+++   ++  RV    +SL   C   I  SL   ++ + KL LP  L   +  +F  TI+  IP P  +++FV  P    + LHC  ++       G   Y LYLE+LG  +P+L   R   ++ E+ I DP  +    +D ++ N  +   ++  NC+          W  K   +  R +++     I++         +   QE  +       K S  +  +T      +D+ ++  LA++ SN+  TKF++      LP  L  + +  + + L P   ++++L E+
Sbjct:  248 LLTVGFTSGDIRLMNNYDDLSPTIVRSGLKDVVVQWCTQGDLLAVAGMEKQNLIPEHVNGNSLKSALIKFYNVRGEHIYTLETP-VQRPITSICWGHRDSRLFLASGPALYVVRVEHRVASLQLLCQQTIATSLSDDKD-ICKLNLPPRLCSYLTTAFIPTIKPPIPDPNNMRDFVSYPTSGNERLHCTMKRTEDDPEVGGPCYTLYLEYLGGLVPVLKGRRISKLRPEFVIMDPKTD--AKTDEIYGNGLISSVIDSCNCSDSSDIELSDDWAAKKSPKISRASKSPKLPRINIEARKSPKLPRAA-QEITRSPRLPIRKPSIGSPSLTRRECPLEDITQHNYLAQVTSNIWGTKFKIVGLAAFLPANLGAVIYKTSLLHLQPRQ-MTIYLPEV 636          

HSP 2 Score: 106.301 bits (264), Expect = 1.585e-22
Identity = 53/134 (39.55%), Postives = 73/134 (54.48%), Query Frame = 3
Query:  219 GWLACGNKTGSVSITKTM--IESDDSTESIPYELPARTNYNLRNSKVPINIVEWNPHHKKLATCSNTGSILVYHNVNNQWSIDLVNERAHAVEVFKWSRSTNQVMIGYTDGLLIIGSVSGHRLWHRMMDMHSAL 614
            GWLA GN  G V +T T+     D +T       P RTN+NLR     + +V WN   +KLATC   G I V+     +WS++LVN+R   V  F WS    Q +I Y DG +++GSVSG R W   +++ S +
Sbjct:   44 GWLATGNGRGVVGVTFTLSHCRRDRNT-------PQRTNFNLRGHNSEVVLVRWNEPFQKLATCDADGGIFVWIQYEGRWSVELVNDRGAQVSDFTWSHDGTQALISYRDGFVLVGSVSGQRHWSSEINLESQI 170          

HSP 3 Score: 53.9138 bits (128), Expect = 1.980e-6
Identity = 20/52 (38.46%), Postives = 37/52 (71.15%), Query Frame = 3
Query: 2931 HSSKRIILKYDNDQILDFSRLENDKYSLEFKYPFSPLQAFAVAIANISRLFR 3086
             S+K   ++ +  Q++ F R++ + Y L+F+YPFS +QAFAVA+AN+++  +
Sbjct: 1493 ESAKNFQIELEGRQVMQFGRIDGNAYILDFQYPFSAVQAFAVALANVTQRLK 1544          
BLAST of TUB like protein 4 vs. Ensembl Xenopus
Match: WDR35 (WD repeat domain 35 [Source:NCBI gene;Acc:100496375])

HSP 1 Score: 74.7146 bits (182), Expect = 7.991e-13
Identity = 38/139 (27.34%), Postives = 69/139 (49.64%), Query Frame = 3
Query:  186 SWMQDHTHSNIGWLACGNKTGSVSITKTMIESDDST-ESIPYELPARTNYNLRNSKVPINIVEWNPHHKKLATCSNTGSILVYHNVNNQWSIDLVNER-AHAVEVFKWSRSTNQVMIGYTDGLLIIGSVSGHRLWHRMM 596
            SW +D      G++ACG ++G + + K  ++++D+  + +        N  L      + +V WN H +KL T    G I+V+      W  +++N R    V    W+    ++ I Y DG +I+GSV G+R+W + +
Sbjct:   21 SWNKDQ-----GYIACGGESGLLKVLKLEMQAEDAKLKGLAAPSNLSMNQTLEGHSGSVQVVTWNEHFQKLTTSDQNGLIIVWMLYKGSWYEEMINNRNKSVVRSMSWNADGQKICIVYEDGAVIVGSVDGNRIWGKEL 154          
BLAST of TUB like protein 4 vs. Ensembl Mouse
Match: Tulp4 (tubby like protein 4 [Source:MGI Symbol;Acc:MGI:1916092])

HSP 1 Score: 134.035 bits (336), Expect = 4.063e-31
Identity = 126/511 (24.66%), Postives = 222/511 (43.44%), Query Frame = 3
Query: 1011 LTCGTWSPNDATIIVGTSIGSLLSFPYAWEXXXXXXXXXXXXXXXXNDSILQVEWSCPPFRRNENGGESITNQDQKYLINSNMKYPHNSFPDSEIPLNDSAASSDLENQLFDNSENFLLAVAFSSGYIYLLRHLEDVDPIKHQTGLSRLCINWNSTGTILAVGGFVRSANLECQCSVKLLSTSAEIIY-------FVPVPSMNKPIMSVTWGHEDTRLFVSASICVHTFRVSSSFSSLTQRCCHIIRNSLKFRQESVDKLCLPSYLHKKIHNSFRKTIQGSIPLPFRLKEFVLLPKQ--DVLHCIFQKVSTGN--GLTKYILYLEFLGASIPLLTASRSRSVKYEYHIYDPSLNQVVDSDPLFPNEEME--VEHSNCTSQC------VWQRKFKHQNRRKTQNCGRNSISVLRDTDSNFSQFLRQESGKIDNCIDSKRSRLNELMTNDDLP-----ENCLLAKLKSNVTLTKFELTSCQICLPRILSRINFGVN-IKLNPSNGVSVHLVEI 2468
            +TCG W+P+D  ++ GT+ G ++                   LL  +D IL + W+ P F   ++      + D     +    YP        IP+ ++                 LL V+F+SG I L+ + +D+ P   ++GL  +   W + G +LAV G  + A L    +  LL ++    Y       F     + +PI+S+ WGH D+RL +++   ++  RV    SSL   C   I ++L+   + V+KL LP  L   +  +F  TI+  IP P  +++FV  P    + LHC  ++       G   Y LYLE+LG  +P+L   R   ++ E+ I DP  +    SD ++ N  +   ++  NC+          W  K   +  R +++     IS+         +   QE  +       K S  +  +T  + P     ++  LA++ SN+  TKF++      LP  L  + +  + + L P   ++++L E+
Sbjct:  170 ITCGIWTPDDQQVLFGTADGQVIVM-------DCHGRMLAHVLLHESDGILSMSWNYPIFLVEDSSESDTDSDDYSPPQDGPAAYP--------IPVQNTKP---------------LLTVSFTSGDISLMNNYDDLSPTVIRSGLKEVVAQWCTQGDLLAVAGMEQQAQLSELPNGPLLKSAMVKFYNVRGEHIFTLDTLVQRPIISICWGHRDSRLLMASGPALYVVRVEHRVSSLQLLCQQAIASTLR-EDKDVNKLTLPPRLCSYLSTAFIPTIKPPIPDPNNMRDFVSYPSAGNERLHCTMKRTEDDPEVGGPCYTLYLEYLGGLVPILKGRRISKLRPEFVIMDPRTDS--KSDEIYGNSLISTVIDSCNCSDSSDIELSDDWAAKKSPKISRSSKSPKLPRISIEARKSPKLPR-AAQEISRSPRLPMRKPSMGSPSLTRREFPFEDITQHNYLAQVTSNIWGTKFKIVGLAAFLPTNLGAVIYKTSLLHLQPRQ-MTIYLPEV 645          

HSP 2 Score: 105.916 bits (263), Expect = 1.563e-22
Identity = 53/134 (39.55%), Postives = 72/134 (53.73%), Query Frame = 3
Query:  219 GWLACGNKTGSVSITKTM--IESDDSTESIPYELPARTNYNLRNSKVPINIVEWNPHHKKLATCSNTGSILVYHNVNNQWSIDLVNERAHAVEVFKWSRSTNQVMIGYTDGLLIIGSVSGHRLWHRMMDMHSAL 614
            GWLA GN  G V +T T      D ST       P R N+NLR     + +V WN  ++KLATC   G I V+     +WS++LVN+R   V  F WS    Q +I Y DG +++GSVSG R W   +++ S +
Sbjct:   44 GWLATGNGRGVVGVTFTSSHCRRDRST-------PQRINFNLRGHNSEVVLVRWNEPYQKLATCDADGGIFVWIQYEGRWSVELVNDRGAQVSDFTWSHDGTQALISYRDGFVLVGSVSGQRHWSSEINLESQI 170          

HSP 3 Score: 53.9138 bits (128), Expect = 1.487e-6
Identity = 20/52 (38.46%), Postives = 37/52 (71.15%), Query Frame = 3
Query: 2931 HSSKRIILKYDNDQILDFSRLENDKYSLEFKYPFSPLQAFAVAIANISRLFR 3086
             S+K   ++ +  Q++ F R++ + Y L+F+YPFS +QAFAVA+AN+++  +
Sbjct: 1496 ESAKNFQIELEGRQVMQFGRIDGNAYILDFQYPFSAVQAFAVALANVTQRLK 1547          
BLAST of TUB like protein 4 vs. Ensembl Mouse
Match: Tulp4 (tubby like protein 4 [Source:MGI Symbol;Acc:MGI:1916092])

HSP 1 Score: 124.79 bits (312), Expect = 2.618e-28
Identity = 104/394 (26.40%), Postives = 182/394 (46.19%), Query Frame = 3
Query: 1362 LLAVAFSSGYIYLLRHLEDVDPIKHQTGLSRLCINWNSTGTILAVGGFVRSANLECQCSVKLLSTSAEIIY-------FVPVPSMNKPIMSVTWGHEDTRLFVSASICVHTFRVSSSFSSLTQRCCHIIRNSLKFRQESVDKLCLPSYLHKKIHNSFRKTIQGSIPLPFRLKEFVLLPKQ--DVLHCIFQKVSTGN--GLTKYILYLEFLGASIPLLTASRSRSVKYEYHIYDPSLNQVVDSDPLFPNEEME--VEHSNCTSQC------VWQRKFKHQNRRKTQNCGRNSISVLRDTDSNFSQFLRQESGKIDNCIDSKRSRLNELMTNDDLP-----ENCLLAKLKSNVTLTKFELTSCQICLPRILSRINFGVN-IKLNPSNGVSVHLVEI 2468
            LL V+F+SG I L+ + +D+ P   ++GL  +   W + G +LAV G  + A L    +  LL ++    Y       F     + +PI+S+ WGH D+RL +++   ++  RV    SSL   C   I ++L+   + V+KL LP  L   +  +F  TI+  IP P  +++FV  P    + LHC  ++       G   Y LYLE+LG  +P+L   R   ++ E+ I DP  +    SD ++ N  +   ++  NC+          W  K   +  R +++     IS+         +   QE  +       K S  +  +T  + P     ++  LA++ SN+  TKF++      LP  L  + +  + + L P   ++++L E+
Sbjct:   64 LLTVSFTSGDISLMNNYDDLSPTVIRSGLKEVVAQWCTQGDLLAVAGMEQQAQLSELPNGPLLKSAMVKFYNVRGEHIFTLDTLVQRPIISICWGHRDSRLLMASGPALYVVRVEHRVSSLQLLCQQAIASTLR-EDKDVNKLTLPPRLCSYLSTAFIPTIKPPIPDPNNMRDFVSYPSAGNERLHCTMKRTEDDPEVGGPCYTLYLEYLGGLVPILKGRRISKLRPEFVIMDPRTDS--KSDEIYGNSLISTVIDSCNCSDSSDIELSDDWAAKKSPKISRSSKSPKLPRISIEARKSPKLPR-AAQEISRSPRLPMRKPSMGSPSLTRREFPFEDITQHNYLAQVTSNIWGTKFKIVGLAAFLPTNLGAVIYKTSLLHLQPRQ-MTIYLPEV 452          

HSP 2 Score: 53.5286 bits (127), Expect = 1.781e-6
Identity = 20/52 (38.46%), Postives = 37/52 (71.15%), Query Frame = 3
Query: 2931 HSSKRIILKYDNDQILDFSRLENDKYSLEFKYPFSPLQAFAVAIANISRLFR 3086
             S+K   ++ +  Q++ F R++ + Y L+F+YPFS +QAFAVA+AN+++  +
Sbjct: 1303 ESAKNFQIELEGRQVMQFGRIDGNAYILDFQYPFSAVQAFAVALANVTQRLK 1354          
BLAST of TUB like protein 4 vs. Ensembl Mouse
Match: Wdr35 (WD repeat domain 35 [Source:MGI Symbol;Acc:MGI:1921932])

HSP 1 Score: 72.4034 bits (176), Expect = 3.001e-12
Identity = 41/139 (29.50%), Postives = 68/139 (48.92%), Query Frame = 3
Query:  186 SWMQDHTHSNIGWLACGNKTGSVSITKTMIESDDSTE---SIPYELPARTNYNLRNSKVPINIVEWNPHHKKLATCSNTGSILVYHNVNNQWSIDLVNER-AHAVEVFKWSRSTNQVMIGYTDGLLIIGSVSGHRLWHR 590
            SW +D      G++ACG + G + + +   ++DDS     + P  L    N NL      + +V WN  ++KL T    G I+V+      W  +++N R    V    W+    ++ I Y DG +I+GSV G+R+W +
Sbjct:   21 SWNKDQ-----GFIACGGEDGLLKVLRLETQTDDSKLRGLAAPSNLS--MNQNLEGHSGAVQVVTWNEQYQKLTTSDQNGLIIVWMLYKGSWYEEMINNRNKSVVRSMSWNADGQKICIVYEDGAVIVGSVDGNRIWGK 152          
BLAST of TUB like protein 4 vs. Ensembl Mouse
Match: Wdr35 (WD repeat domain 35 [Source:MGI Symbol;Acc:MGI:1921932])

HSP 1 Score: 72.4034 bits (176), Expect = 3.088e-12
Identity = 41/139 (29.50%), Postives = 68/139 (48.92%), Query Frame = 3
Query:  186 SWMQDHTHSNIGWLACGNKTGSVSITKTMIESDDSTE---SIPYELPARTNYNLRNSKVPINIVEWNPHHKKLATCSNTGSILVYHNVNNQWSIDLVNER-AHAVEVFKWSRSTNQVMIGYTDGLLIIGSVSGHRLWHR 590
            SW +D      G++ACG + G + + +   ++DDS     + P  L    N NL      + +V WN  ++KL T    G I+V+      W  +++N R    V    W+    ++ I Y DG +I+GSV G+R+W +
Sbjct:   21 SWNKDQ-----GFIACGGEDGLLKVLRLETQTDDSKLRGLAAPSNLS--MNQNLEGHSGAVQVVTWNEQYQKLTTSDQNGLIIVWMLYKGSWYEEMINNRNKSVVRSMSWNADGQKICIVYEDGAVIVGSVDGNRIWGK 152          
BLAST of TUB like protein 4 vs. Ensembl Mouse
Match: Wdr35 (WD repeat domain 35 [Source:MGI Symbol;Acc:MGI:1921932])

HSP 1 Score: 61.6178 bits (148), Expect = 2.944e-10
Identity = 36/129 (27.91%), Postives = 60/129 (46.51%), Query Frame = 3
Query:  186 SWMQDHTHSNIGWLACGNKTGSVSITKTMIESDDST-ESIPYELPARTNYNLRNSKVPINIVEWNPHHKKLATCSNTGSILVYHNVNNQWSIDLVNER-AHAVEVFKWSRSTNQVMIGYTDGLLIIGSV 566
            SW +D      G++ACG + G + + +   ++DDS    +        N NL      + +V WN  ++KL T    G I+V+      W  +++N R    V    W+    ++ I Y DG +I+GSV
Sbjct:   21 SWNKDQ-----GFIACGGEDGLLKVLRLETQTDDSKLRGLAAPSNLSMNQNLEGHSGAVQVVTWNEQYQKLTTSDQNGLIIVWMLYKGSWYEEMINNRNKSVVRSMSWNADGQKICIVYEDGAVIVGSV 144          
BLAST of TUB like protein 4 vs. UniProt/SwissProt
Match: sp|Q9JIL5|TULP4_MOUSE (Tubby-related protein 4 OS=Mus musculus OX=10090 GN=Tulp4 PE=1 SV=1)

HSP 1 Score: 134.035 bits (336), Expect = 2.479e-30
Identity = 126/511 (24.66%), Postives = 222/511 (43.44%), Query Frame = 3
Query: 1011 LTCGTWSPNDATIIVGTSIGSLLSFPYAWEXXXXXXXXXXXXXXXXNDSILQVEWSCPPFRRNENGGESITNQDQKYLINSNMKYPHNSFPDSEIPLNDSAASSDLENQLFDNSENFLLAVAFSSGYIYLLRHLEDVDPIKHQTGLSRLCINWNSTGTILAVGGFVRSANLECQCSVKLLSTSAEIIY-------FVPVPSMNKPIMSVTWGHEDTRLFVSASICVHTFRVSSSFSSLTQRCCHIIRNSLKFRQESVDKLCLPSYLHKKIHNSFRKTIQGSIPLPFRLKEFVLLPKQ--DVLHCIFQKVSTGN--GLTKYILYLEFLGASIPLLTASRSRSVKYEYHIYDPSLNQVVDSDPLFPNEEME--VEHSNCTSQC------VWQRKFKHQNRRKTQNCGRNSISVLRDTDSNFSQFLRQESGKIDNCIDSKRSRLNELMTNDDLP-----ENCLLAKLKSNVTLTKFELTSCQICLPRILSRINFGVN-IKLNPSNGVSVHLVEI 2468
            +TCG W+P+D  ++ GT+ G ++                   LL  +D IL + W+ P F   ++      + D     +    YP        IP+ ++                 LL V+F+SG I L+ + +D+ P   ++GL  +   W + G +LAV G  + A L    +  LL ++    Y       F     + +PI+S+ WGH D+RL +++   ++  RV    SSL   C   I ++L+   + V+KL LP  L   +  +F  TI+  IP P  +++FV  P    + LHC  ++       G   Y LYLE+LG  +P+L   R   ++ E+ I DP  +    SD ++ N  +   ++  NC+          W  K   +  R +++     IS+         +   QE  +       K S  +  +T  + P     ++  LA++ SN+  TKF++      LP  L  + +  + + L P   ++++L E+
Sbjct:  170 ITCGIWTPDDQQVLFGTADGQVIVM-------DCHGRMLAHVLLHESDGILSMSWNYPIFLVEDSSESDTDSDDYSPPQDGPAAYP--------IPVQNTKP---------------LLTVSFTSGDISLMNNYDDLSPTVIRSGLKEVVAQWCTQGDLLAVAGMEQQAQLSELPNGPLLKSAMVKFYNVRGEHIFTLDTLVQRPIISICWGHRDSRLLMASGPALYVVRVEHRVSSLQLLCQQAIASTLR-EDKDVNKLTLPPRLCSYLSTAFIPTIKPPIPDPNNMRDFVSYPSAGNERLHCTMKRTEDDPEVGGPCYTLYLEYLGGLVPILKGRRISKLRPEFVIMDPRTDS--KSDEIYGNSLISTVIDSCNCSDSSDIELSDDWAAKKSPKISRSSKSPKLPRISIEARKSPKLPR-AAQEISRSPRLPMRKPSMGSPSLTRREFPFEDITQHNYLAQVTSNIWGTKFKIVGLAAFLPTNLGAVIYKTSLLHLQPRQ-MTIYLPEV 645          

HSP 2 Score: 106.301 bits (264), Expect = 9.546e-22
Identity = 53/134 (39.55%), Postives = 72/134 (53.73%), Query Frame = 3
Query:  219 GWLACGNKTGSVSITKTM--IESDDSTESIPYELPARTNYNLRNSKVPINIVEWNPHHKKLATCSNTGSILVYHNVNNQWSIDLVNERAHAVEVFKWSRSTNQVMIGYTDGLLIIGSVSGHRLWHRMMDMHSAL 614
            GWLA GN  G V +T T      D ST       P R N+NLR     + +V WN  ++KLATC   G I V+     +WS++LVN+R   V  F WS    Q +I Y DG +++GSVSG R W   +++ S +
Sbjct:   44 GWLATGNGRGVVGVTFTSSHCRRDRST-------PQRINFNLRGHNSEVVLVRWNEPYQKLATCDADGGIFVWIQYEGRWSVELVNDRGAQVSDFTWSHDGTQALISYRDGFVLVGSVSGQRHWSSEINLESQI 170          
BLAST of TUB like protein 4 vs. UniProt/SwissProt
Match: sp|Q9NRJ4|TULP4_HUMAN (Tubby-related protein 4 OS=Homo sapiens OX=9606 GN=TULP4 PE=1 SV=2)

HSP 1 Score: 127.872 bits (320), Expect = 1.950e-28
Identity = 124/511 (24.27%), Postives = 218/511 (42.66%), Query Frame = 3
Query: 1011 LTCGTWSPNDATIIVGTSIGSLLSFPYAWEXXXXXXXXXXXXXXXXNDSILQVEWSCPPFRRNENGGESITNQDQKYLINSNMKYPHNSFPDSEIPLNDSAASSDLENQLFDNSENFLLAVAFSSGYIYLLRHLEDVDPIKHQTGLSRLCINWNSTGTILAVGGFVRSANLECQCSVKLLSTSAEIIY-------FVPVPSMNKPIMSVTWGHEDTRLFVSASICVHTFRVSSSFSSLTQRCCHIIRNSLKFRQESVDKLCLPSYLHKKIHNSFRKTIQGSIPLPFRLKEFVLLPKQ--DVLHCIFQKVSTGN--GLTKYILYLEFLGASIPLLTASRSRSVKYEYHIYDPSLNQVVDSDPLFPNEEME--VEHSNCTSQC------VWQRKFKHQNRRKTQNCGRNSISVLRDTDSNFSQFLRQESGKIDNCIDSKRSRLNELMTNDDLP-----ENCLLAKLKSNVTLTKFELTSCQICLPRILSRINFGVN-IKLNPSNGVSVHLVEI 2468
            +TCG W+P+D  ++ GT+ G ++                   LL  +D +L + W+ P F   ++      + D     +    YP        IP+ +                  LL V+F+SG I L+ + +D+ P   ++GL  +   W + G +LAV G  R   L    +  LL ++    Y       F     + +PI+S+ WGH D+RL +++   ++  RV    SSL   C   I ++L+   + V KL LP  L   +  +F  TI+  IP P  +++FV  P    + LHC  ++       G   Y LYLE+LG  +P+L   R   ++ E+ I DP  +     D ++ N  +   ++  NC+          W  K   +  R +++     IS+         +   QE  +       K S  +  +T  + P     ++  LA++ SN+  TKF++      LP  L  + +  + + L P   ++++L E+
Sbjct:  170 ITCGIWTPDDQQVLFGTADGQVIVM-------DCHGRMLAHVLLHESDGVLGMSWNYPIFLVEDSSESDTDSDDYAPPQDGPAAYP--------IPVQNIKP---------------LLTVSFTSGDISLMNNYDDLSPTVIRSGLKEVVAQWCTQGDLLAVAGMERQTQLGELPNGPLLKSAMVKFYNVRGEHIFTLDTLVQRPIISICWGHRDSRLLMASGPALYVVRVEHRVSSLQLLCQQAIASTLR-EDKDVSKLTLPPRLCSYLSTAFIPTIKPPIPDPNNMRDFVSYPSAGNERLHCTMKRTEDDPEVGGPCYTLYLEYLGGLVPILKGRRISKLRPEFVIMDPRTDS--KPDEIYGNSLISTVIDSCNCSDSSDIELSDDWAAKKSPKISRASKSPKLPRISIEARKSPKLPRAA-QELSRSPRLPLRKPSVGSPSLTRREFPFEDITQHNYLAQVTSNIWGTKFKIVGLAAFLPTNLGAVIYKTSLLHLQPRQ-MTIYLPEV 645          

HSP 2 Score: 106.301 bits (264), Expect = 1.038e-21
Identity = 53/134 (39.55%), Postives = 72/134 (53.73%), Query Frame = 3
Query:  219 GWLACGNKTGSVSITKTM--IESDDSTESIPYELPARTNYNLRNSKVPINIVEWNPHHKKLATCSNTGSILVYHNVNNQWSIDLVNERAHAVEVFKWSRSTNQVMIGYTDGLLIIGSVSGHRLWHRMMDMHSAL 614
            GWLA GN  G V +T T      D ST       P R N+NLR     + +V WN  ++KLATC   G I V+     +WS++LVN+R   V  F WS    Q +I Y DG +++GSVSG R W   +++ S +
Sbjct:   44 GWLATGNGRGVVGVTFTSSHCRRDRST-------PQRINFNLRGHNSEVVLVRWNEPYQKLATCDADGGIFVWIQYEGRWSVELVNDRGAQVSDFTWSHDGTQALISYRDGFVLVGSVSGQRHWSSEINLESQI 170          
BLAST of TUB like protein 4 vs. UniProt/SwissProt
Match: sp|Q8BND3|WDR35_MOUSE (WD repeat-containing protein 35 OS=Mus musculus OX=10090 GN=Wdr35 PE=1 SV=3)

HSP 1 Score: 72.4034 bits (176), Expect = 2.160e-11
Identity = 41/139 (29.50%), Postives = 68/139 (48.92%), Query Frame = 3
Query:  186 SWMQDHTHSNIGWLACGNKTGSVSITKTMIESDDSTE---SIPYELPARTNYNLRNSKVPINIVEWNPHHKKLATCSNTGSILVYHNVNNQWSIDLVNER-AHAVEVFKWSRSTNQVMIGYTDGLLIIGSVSGHRLWHR 590
            SW +D      G++ACG + G + + +   ++DDS     + P  L    N NL      + +V WN  ++KL T    G I+V+      W  +++N R    V    W+    ++ I Y DG +I+GSV G+R+W +
Sbjct:   21 SWNKDQ-----GFIACGGEDGLLKVLRLETQTDDSKLRGLAAPSNLS--MNQNLEGHSGAVQVVTWNEQYQKLTTSDQNGLIIVWMLYKGSWYEEMINNRNKSVVRSMSWNADGQKICIVYEDGAVIVGSVDGNRIWGK 152          
BLAST of TUB like protein 4 vs. UniProt/SwissProt
Match: sp|A6N6J5|WDR35_RAT (WD repeat-containing protein 35 OS=Rattus norvegicus OX=10116 GN=Wdr35 PE=1 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 2.247e-11
Identity = 41/139 (29.50%), Postives = 68/139 (48.92%), Query Frame = 3
Query:  186 SWMQDHTHSNIGWLACGNKTGSVSITKTMIESDDSTE---SIPYELPARTNYNLRNSKVPINIVEWNPHHKKLATCSNTGSILVYHNVNNQWSIDLVNER-AHAVEVFKWSRSTNQVMIGYTDGLLIIGSVSGHRLWHR 590
            SW +D      G++ACG + G + + +   ++DDS     + P  L    N NL      + +V WN  ++KL T    G I+V+      W  +++N R    V    W+    ++ I Y DG +I+GSV G+R+W +
Sbjct:   21 SWNKDQ-----GFIACGGEDGLLKVLRLETQTDDSKLRGLAAPSNLS--MNQNLEGHSGAVQVVTWNEQYQKLTTSDQNGLIIVWMLYKGSWYEEMINNRNKSVVRSMSWNADGQKICIVYEDGAVIVGSVDGNRIWGK 152          
BLAST of TUB like protein 4 vs. UniProt/SwissProt
Match: sp|Q9P2L0|WDR35_HUMAN (WD repeat-containing protein 35 OS=Homo sapiens OX=9606 GN=WDR35 PE=1 SV=3)

HSP 1 Score: 68.9366 bits (167), Expect = 2.426e-10
Identity = 37/128 (28.91%), Postives = 63/128 (49.22%), Query Frame = 3
Query:  219 GWLACGNKTGSVSITKTMIESDDSTE---SIPYELPARTNYNLRNSKVPINIVEWNPHHKKLATCSNTGSILVYHNVNNQWSIDLVNER-AHAVEVFKWSRSTNQVMIGYTDGLLIIGSVSGHRLWHR 590
            G++ACG + G + + K   ++DD+     + P  L    N  L      + +V WN  ++KL T    G I+V+      W  +++N R    V    W+    ++ I Y DG +I+GSV G+R+W +
Sbjct:   27 GFIACGGEDGLLKVLKLETQTDDAKLRGLAAPSNLS--MNQTLEGHSGSVQVVTWNEQYQKLTTSDENGLIIVWMLYKGSWIEEMINNRNKSVVRSMSWNADGQKICIVYEDGAVIVGSVDGNRIWGK 152          
BLAST of TUB like protein 4 vs. TrEMBL
Match: A0A210QDL8 (Tubby-related protein 4 OS=Mizuhopecten yessoensis OX=6573 GN=KP79_PYT07091 PE=3 SV=1)

HSP 1 Score: 204.142 bits (518), Expect = 7.064e-50
Identity = 144/503 (28.63%), Postives = 239/503 (47.51%), Query Frame = 3
Query:  999 DMPTLTCGTWSPNDATIIVGTSIGSLLSFPYAWEXXXXXXXXXXXXXXXXNDS--ILQVEWSCPPFRRNENGGESITNQDQKYLINSNMKYPHNSFPDSEIPLNDSAASSDLENQLFDNSENFLLAVAFSSGYIYLLRHLEDVDPIKHQTGLSRLCINWNSTGTILAVGGFVRSANLECQCSVKLLSTSAEIIYFVPVPSMNKPIMSVTWGHEDTRLFVSASICVHTFRVSSSFSSLTQRCCHIIRNSLKFRQESVDKLCLPSYLHKKIHNSFRKTIQGSIPLPFRLKEFVLL--PKQDVLHCIFQKVSTGNGLTKYILYLEFLGASIPLLTASRSRSVKYEYHIYDPSLNQVVDSDPLFPNEEMEVEHSNCTSQCVWQRKFKHQNRRKTQNCGRNSISVLRDTDSNFSQFLRQESGKIDNCIDSKRSRLNELMTNDDLPENCLLAKLKSNVTLTKFELTSCQICLPRILSRINFGVNIKLNPSNGVSVHLVEISNSTGQKMT 2495
            D  T+TCG WSP+D  +I GT+ G L+                    + +NDS  I  + WSC  F  +E+  +S T          N+ +P             S ASS +++       N +LA+ F  G IYL+ + +D+ P    T L+ L ++W++ G ILAVGGF+R  NL+C+  +   S    +++++ +PS  KP+ ++ WGH D RLF+++   +H   V+   + L   C  II++++  +++ V KL LPS L   I   F  TI+  +P PF+L+EFV    P  + L C   +         Y LYLE+LG  IPLL   R+  ++ ++ I+DP +          P    E  + N   +  ++      +   T  CG   +   +    +F       + K++  I  +   L+EL+ ND LPE+  L ++ SN+  TKF +T     LP  L  I +  ++       +++ + EI  S GQ ++
Sbjct:  170 DNTTITCGVWSPDDQKVIFGTTGGQLIVM---------SSTGAMLTQVIINDSMEISTMAWSCEKFNMDESDAKSSTTD--------NISHP-------------SEASSAVDS-------NHVLAICFKYGAIYLMSNYDDICPRVVHTLLTGLRMDWSNCGEILAVGGFIRLPNLQCRNELHFYSRDGRLLHWISIPSQGKPLTALCWGHNDKRLFLASGFNLHVAWVTKQVAPLQFLCQRIIQDNIT-QEKKVSKLPLPSRLRHNIQALFSPTIKSYVPDPFKLREFVTSTPPGNERLFCTMVRHGEETSGGHYTLYLEYLGGLIPLLKGKRASKLRPDFVIFDPKIK---------PKTNPEATNGNSQYEAEYESDSDSDSDISTDGCGSPRMQ-RKKRPKHF------RAAKVERSITFR--TLDELLYNDSLPESSKLVEVTSNIWGTKFHITGTMPLLPEDLGHILYKTSLLHLQPRQMTITVTEI-RSDGQLLS 615          

HSP 2 Score: 107.842 bits (268), Expect = 7.177e-20
Identity = 50/128 (39.06%), Postives = 74/128 (57.81%), Query Frame = 3
Query:  219 GWLACGNKTGSVSITKTMIESDDSTESIPYELPARTNYNLRNSKVPINIVEWNPHHKKLATCSNTGSILVYHNVNNQWSIDLVNERAHAVEVFKWSRSTNQVMIGYTDGLLIIGSVSGHRLWHRMMDM 602
            GWLA GN  G V IT T      ++    Y+ P R+N+NLR  +  + +V WN  ++KLATC + G I V+     +WSI+L+N+R   V  F WS      +I Y DG +++GSV+G R W  M+++
Sbjct:   48 GWLASGNAKGIVGITFT------TSHCRKYDAPQRSNFNLRGHRSEVILVRWNEPYQKLATCDSQGVIFVWIKHEGRWSIELINDRNSQVTDFAWSHDGRMALICYCDGFVLVGSVAGQRYWSSMLNL 169          
BLAST of TUB like protein 4 vs. TrEMBL
Match: A0A2C9KB24 (SOCS box domain-containing protein OS=Biomphalaria glabrata OX=6526 GN=106060626 PE=3 SV=1)

HSP 1 Score: 185.267 bits (469), Expect = 8.737e-44
Identity = 133/487 (27.31%), Postives = 212/487 (43.53%), Query Frame = 3
Query:  999 DMPTLTCGTWSPNDATIIVGTSIGSLLSFPYAWEXXXXXXXXXXXXXXXXNDSILQVEWSCPPFRRNE-NGGESITNQDQKYLINSNMKYPHNSFPDSEIPLNDSAASSDLENQLFDNSENFLLAVAFSSGYIYLLRHLEDVDPIKHQTGLSRLCINWNSTGTILAVGGFVRSANLECQCSVKLLSTSAEIIYFVPVPSMNKPIMSVTWGHEDTRLFVSASICVHTFRVSSSFSSLTQRCCHIIRNSLKFRQESVDKLCLPSYLHKKIHNSFRKTIQGSIPLPFRLKEFVLLP--KQDVLHCIFQKVSTGNGLTKYILYLEFLGASIPLLTASRSRSVKYEYHIYDPSLNQVVDSDPLFPN---------EEMEVEHSNCTSQCVWQRKFKHQNRRKTQNCGRNSISVLRDTDSNFSQFLRQESGKIDNCIDSKRSRLNELMTNDDLPENCLLAKLKSNVTLTKFELTSCQICLPRILSRINFGVNI 2423
            D  ++TCG WSP+D  ++ GT  G ++                    +     I  + WSC  F   E   G   T++  +                                    +S++ +LAV F  G I+L+   +DV P+   T L+ + I+W++ G  LAVGGFVR  NL+C+  V   S    +I++V +PS  KP+ ++TWGH D RLF++A   ++   VS   + L   C  ++  +++  + +V++L +P  L   +   F  TI+  IP P RL+EFV  P    + L C   +         Y LYLE+LG  +PLL   R+  ++ ++ IYDP +     S+   P           E EV    C S        + Q RR+ +        V R    N S   R                L+ELM +D+LPE   L ++ SN+  TKF++T     LP  L ++ +  ++
Sbjct:   83 DNTSITCGVWSPDDQQVMFGTLDGQIIVM-------SSTGAVVTQVTVHEGFEISSMAWSCERFNMEEAETGRETTDKGSE-----------------------------------SSSKSHVLAVCFKYGDIFLMSGYDDVCPVMIYTTLTGIKIDWSNCGEYLAVGGFVRLPNLQCRNEVHFYSKDGSLIHWVTIPSQGKPLTAMTWGHNDKRLFLAAGCHMYVAWVSKQVAPLQFLCQRVVHQTVR-TERAVERLPIPQRLKCGVKALFSPTIKSYIPDPLRLREFVCSPPPGGERLFCTMIRHGEETSGGHYTLYLEYLGGLVPLLKGKRASKLRPDFIIYDPKIKSSGKSEVAQPGGIESDTSSESEAEVYTDGCGSP-------RMQRRRRNK--------VFRAERVNRSITFRT---------------LDELMYDDNLPETNRLVEVTSNIWGTKFQITGLASSLPACLGQVVYKTSL 496          

HSP 2 Score: 78.5666 bits (192), Expect = 9.017e-11
Identity = 32/82 (39.02%), Postives = 50/82 (60.98%), Query Frame = 3
Query:  357 INIVEWNPHHKKLATCSNTGSILVYHNVNNQWSIDLVNERAHAVEVFKWSRSTNQVMIGYTDGLLIIGSVSGHRLWHRMMDM 602
            + +V WN  ++KLATC + G I V+     +WSI+L+N+R   V  F WS      +I Y DG +++GSV+G R W  M+++
Sbjct:    1 VVLVRWNEPYQKLATCDSRGVIFVWIKHEGRWSIELINDRNSQVTDFAWSHDGRMALICYRDGFILVGSVAGQRYWSSMLNL 82          
BLAST of TUB like protein 4 vs. TrEMBL
Match: A0A2T7P695 (Tub domain-containing protein OS=Pomacea canaliculata OX=400727 GN=C0Q70_11541 PE=3 SV=1)

HSP 1 Score: 181.03 bits (458), Expect = 1.018e-42
Identity = 140/505 (27.72%), Postives = 224/505 (44.36%), Query Frame = 3
Query:  999 DMPTLTCGTWSPNDATIIVGTSIGSLLSFPYAWEXXXXXXXXXXXXXXXXNDSILQVEWSCPPFRRNENGGESITNQDQKYLINSNMKYPHNSFPDSEIPLNDSAASSDLENQLFDNSENFLLAVAFSSGYIYLLRHLEDVDPIKHQTGLSRLCINWNSTGTILAVGGFVRSANLECQCSVKLLSTSAEIIYFVPVPSMNKPIMSVTWGHEDTRLFVSASICVHTFRVSSSFSS---LTQRCCH-IIRNSLKFRQESVDKLCLPSYLHKKIHNSFRKTIQGSIPLPFRLKEFVLL--PKQDVLHCIFQKVSTGNGLTKYILYLEFLGASIPLLTASRSRSVKYEYHIYDPSLNQVV----DSDPLFPNEEMEVEHSNCTSQCVWQRKFKHQNRRKTQNCGRNSISVLRDTDSNFSQFLRQESGKIDNCIDSKRSRLNELMTNDDLPENCLLAKLKSNVTLTKFELTSCQICLPRILSRINFGVN-IKLNPSNGVSVHLVEISNST 2480
            D  ++ CG WSP+D  ++ GTS G L+                    +     I  + WSC  F   E                                 ++   +   +  L    +N  LAV F  G I+L+    D  P    T L+ L I W++ G  LAVGGF+R  NL+C+  +   S   + I++VPVPS  KP+ ++TWGH D RLF++A   ++   VS   +    L+QR  H ++R+     ++SV +L LP  L + + + F  T++  IP PF+L+ F+    P ++ + C   +         Y LYLE+LG  +PLL   R+  ++ ++ I+DP +        DS  +  +   E E    T  C   R    Q RR+                    +  R E  K+D  I  +   L+EL+ ND+LPE   L ++ SN+  TKF++T     LP +L ++ +  + + L P   +++ L EI  ST
Sbjct:   51 DNTSILCGIWSPDDQQVMFGTSDGQLIVM-------SSTGAMVSQVTINEGFEITSMTWSCEKFNMEE--------------------------------ADNRGGAPYGQRSLEGREKNHTLAVCFKYGDIFLMSDYHDPCPRIISTSLTGLKIEWSNCGQYLAVGGFLRLPNLQCRNELHFYSCEGKRIHWVPVPSQGKPLTALTWGHNDKRLFLAAGCQLYVAWVSKQVAPLQFLSQRVIHRLVRH-----EKSVLQLPLPVRLRQGVQSLFSPTVKSYIPDPFKLRAFISTPPPGRERMFCTMIRHGDETSGGHYTLYLEYLGGLVPLLKGKRASKLRPDFVIFDPKIRTSSRSQGDSSAVQTDSSSESEADVVTDGCGSPRM---QRRRR-------------------HKVFRAE--KVDRSITFR--TLDELLYNDNLPETNKLVEVTSNIWGTKFQITGVASYLPSMLGQVVYKTSLLHLQPRQ-MTITLQEIYGST 484          
BLAST of TUB like protein 4 vs. TrEMBL
Match: K1QME8 (Tubby-related protein 4 OS=Crassostrea gigas OX=29159 GN=CGI_10025580 PE=3 SV=1)

HSP 1 Score: 174.866 bits (442), Expect = 6.113e-41
Identity = 123/404 (30.45%), Postives = 199/404 (49.26%), Query Frame = 3
Query: 1317 SSDLENQLFDNSENFLLAVAFSSGYIYLLRHLEDVDPIKHQTGL-SRLCINWNSTGTILAVGGFVRSANLECQCSVKLLSTSAEIIYFVPVPSMNKPIMSVTWGHEDTRLFVSASICVHTFRVSSSFSSLTQRCCHIIRNSLKFRQESVDKLCLPSYLHKKIHNSFRKTIQGSIPLPFRLKEFVLL--PKQDVLHCIFQKVSTGNGLTKYILYLEFLGASIPLLTASRSRSVKYEYHIYDPSLNQVVDSDPLFPNEEMEVEHSNCTSQCVWQRKFKHQNRR-KTQNCGRNSISVLRDTDSNFSQFLRQESGKIDNCIDSKRSRLNELMTNDDLPENCLLAKLKSNVTLTKFELTSCQICLPRILSRINFGVNIK-LNPSNGVSVHLVEISNSTGQKMTNYYRDI 2513
            SS  +N+ F    + +LAV F SG IYL+ + +D+ P    T L   + ++W++ G +LAVGGF R  NL+CQ  V   +   ++++ V +P   KP+ ++ WGH D RLFV+A   +H   V      L   C  II+  +K  ++ + KL LP+ LH  +   F  TI+  IP PF+L+EF+    P  + L C   +         Y LYLE+LG  IPLL   R+  ++ ++ IYDP +     S         E +HS    +CV++      +    +  CG       R       +  R +  K+D  I  +   L+EL+ ND LPE+  L ++ SN+  TKF+LT     LP  L   +  V+++ +   +G+ V      ++  +  ++ + DI
Sbjct:   41 SSTEQNENFTAPTDAVLAVCFKSGAIYLMSNYDDLHPRIIYTHLKGSIKLDWSNCGEVLAVGGFTRLPNLQCQNEVHFYTKEGQLLHRVYLPCQGKPLTALCWGHNDRRLFVAAGFNLHVAWVMKKVPHLQFLCQRIIQICIK-SEKLIRKLPLPARLHHSVQTMFSPTIKSYIPDPFKLREFICSPPPASERLFCTMIRHGEETSGGHYTLYLEYLGGLIPLLKGKRASKLRPDFVIYDPKIGAKACS---------EDQHS--LPECVFEASLTDSDEEIPSDGCGSP-----RMQRKKRPKIFRPK--KVDRSITFR--TLDELLYNDSLPESNKLVEVTSNIWGTKFKLTGLAPFLPEDLDE-DLPVDLEDMGQEDGILVSETYSGDNVNRGTSHVFMDI 422          
BLAST of TUB like protein 4 vs. TrEMBL
Match: R7T9E4 (Tub domain-containing protein OS=Capitella teleta OX=283909 GN=CAPTEDRAFT_225536 PE=3 SV=1)

HSP 1 Score: 166.007 bits (419), Expect = 7.075e-38
Identity = 134/498 (26.91%), Postives = 228/498 (45.78%), Query Frame = 3
Query: 1008 TLTCGTWSPNDATIIVGTSIGSLLSFPYAWEXXXXXXXXXXXXXXXXNDSILQVEWSCPPFRRNENGGESITNQDQKYLINSNMKYPHNSFPDSEIPLNDSAASSDLENQLFDNSENFLLAVAFSSGYIYLLRHLEDVDPIKHQTGLSRLCINWNSTGTILAVGGFVRSANLECQCSVKLLSTSAEIIYFVPVPSMNKPIMSVTWGHEDTRLFVSASICVHTFRVSSSFSSL---TQRCCHIIRNSLKFRQESVDKLCLPSYLHKKIHNSFRKTIQGSIPLPFRLKEFVLL--PKQDVLHC-IFQKVSTGNGLTKYILYLEFLGASIPLLTASRSRSVKYEYHIYDP----SLNQVVDSDPLFPNEEMEVEHSNCTSQCVWQRKFKHQNRRKTQNCGRNSISVLRDTDSNFSQFLRQESGKIDNCIDSKRSRLNELMTNDDLPENCLLAKLKSNVTLTKFELTSCQICLPRILSRINFGVNIKLNPSNGVSVHLVEIS 2471
            ++TC TW+P+D  ++ GT+ G  +    A               L  +  +  +EW+C  F  +++       +D     +S+     ++    + P+  S                 +LAV F  G+I+L+    D  P   +T L  L + W++ G +LAV G     +L CQ  ++L +++  + + +P+    KP++++TWGH D RLF++    V+T RV    SSL   +QRC   I+  LK     + KL LPS + +   + F  TI+G  P P +L+EFV +  P ++ +HC + +   T +    Y LYLE+LG  IPLL   R+  ++ E+ IYDP    ++ QV D   L  NEE     S      V              NC    +   R       Q  R++S       D K   + ++M +D+LPE+  L ++ SN+  TKF +      LP  L  + +  ++       +++ ++E+S
Sbjct:  104 SITCCTWTPDDEKVMFGTTDGRAIVMTSA-------GAMVTPVNLEDDQEVQSMEWNCEKFFLDDSQHSDAAGEDTSSSSDSSAAASSSA----KAPVRKSKRG------------EHILAVCFLDGFIHLMSSYNDAFPKVIETNLVGLRMEWSNEGDVLAVTGHTLLPDLSCQNELRLYTSTGHLRFLMPITGQTKPVLALTWGHLDHRLFLAIDCFVYTARVDKQISSLQYFSQRC---IQAQLKHEAHCL-KLPLPSRIQESFLSLFTSTIRGFYPEPHKLREFVCVPPPGEERVHCTMIRHGETEDTGEHYTLYLEYLGGLIPLLKGKRASKLRPEFVIYDPKIKGAVQQVEDDTLLLSNEEESNRESESDEDFV------------MDNCASPRMQRKRQQRRRLDQVARRDSAP-----DLK--SMEDMMYDDNLPESAKLVEVTSNIWGTKFRIHGLASFLPEDLGEVVYKTSLLHLQPRQMTISIMELS 555          

HSP 2 Score: 78.1814 bits (191), Expect = 1.062e-10
Identity = 32/84 (38.10%), Postives = 50/84 (59.52%), Query Frame = 3
Query:  357 INIVEWNPHHKKLATCSNTGSILVYHNVNNQWSIDLVNERAHAVEVFKWSRSTNQVMIGYTDGLLIIGSVSGHRLWHRMMDMHS 608
            + +V WN  ++KLATC  +G I V+     +WSI+L+N+R   V  F WS   +  +I Y DG ++ GSV+G R W  M+++  
Sbjct:   19 VILVRWNEPYQKLATCDTSGVIFVWIKHEGRWSIELINDRNSQVTDFAWSHEGHMALICYRDGFVLAGSVAGQRYWSTMINLEG 102          
BLAST of TUB like protein 4 vs. Ensembl Cavefish
Match: tulp4b (TUB like protein 4b [Source:ZFIN;Acc:ZDB-GENE-130530-708])

HSP 1 Score: 137.502 bits (345), Expect = 2.702e-32
Identity = 101/359 (28.13%), Postives = 161/359 (44.85%), Query Frame = 3
Query: 1011 LTCGTWSPNDATIIVGTSIGSLLSFPYAWEXXXXXXXXXXXXXXXXNDSILQVEWSCPPFRRNENGGESITNQDQKYLINSNMKYPHNSFPDSEIPLNDSAASSDLENQLFDNSENFLLAVAFSSGYIYLLRHLEDVDPIKHQTGLSRLCINWNSTGTILAVGGFVRSA-NLECQCS-------VKLLSTSAEIIYFVPVPSMNKPIMSVTWGHEDTRLFVSASICVHTFRVSSSFSSLTQRCCHIIRNSLKFRQESVDKLCLPSYLHKKIHNSFRKTIQGSIPLPFRLKEFVLLPKQ--DVLHCIFQKVSTG--NGLTKYILYLEFLGASIPLLTASRSRSVKYEYHIYDPSLNQVVD 2051
            +TCG W+P+D  ++ GT+ G ++                   LL  +D I+ + W+CP F   E+  ES T+ D+          P                               LL V+F SG I L+ + +D+ P   ++GL  + + W S G +LAV G  R     E  C+       VK  +   E IY +  P+  +PI ++ WGH D+RLF++    ++  RV    +SL   C   I ++L+  +  V KL +PS L   + ++F  TI+  IP P  +++FV  P    + LHC  ++       G   Y LYLE LG  +P+L   R   ++ E+ I DP ++   D
Sbjct:  170 ITCGIWTPDDQQVLFGTADGQVIVM-------DCHGRMLAHVLLHESDGIVGMSWNCPDFLV-EDSTESDTDTDENAQSQGRNVKP-------------------------------LLTVSFISGDISLMNNYDDLSPHVIRSGLKDVEVQWCSQGDLLAVAGMERHGLPSESACASIMRNALVKFYNVQGEHIYTLETPA-QRPITTICWGHRDSRLFLACGPALYVVRVEHRVASLQLLCQQGIASALR-EERDVGKLNMPSLLCSYVTSAFIPTIKPPIPDPNNIRDFVSYPTAGNERLHCTMKRAEDNPEAGGPCYTLYLEHLGGLVPILKGRRISKLRPEFVIMDPKMDSKTD 487          

HSP 2 Score: 103.605 bits (257), Expect = 7.343e-22
Identity = 52/134 (38.81%), Postives = 71/134 (52.99%), Query Frame = 3
Query:  219 GWLACGNKTGSVSITKTM--IESDDSTESIPYELPARTNYNLRNSKVPINIVEWNPHHKKLATCSNTGSILVYHNVNNQWSIDLVNERAHAVEVFKWSRSTNQVMIGYTDGLLIIGSVSGHRLWHRMMDMHSAL 614
            GWLA GN  G V +T T      D +T       P R N+NLR     + +V WN   +KLATC   G I V+     +WS++LVN+R   V  F WS    Q +I Y DG +++GSVSG R W   +++ S +
Sbjct:   44 GWLATGNARGVVGVTFTSSHCRRDRNT-------PQRINFNLRGHNSEVVLVRWNEPFQKLATCDMEGGIFVWIQYEGRWSVELVNDRGAQVSDFTWSHDGTQALIAYRDGFVLVGSVSGQRHWSSEINLESQI 170          

HSP 3 Score: 55.4546 bits (132), Expect = 3.408e-7
Identity = 21/52 (40.38%), Postives = 37/52 (71.15%), Query Frame = 3
Query: 2931 HSSKRIILKYDNDQILDFSRLENDKYSLEFKYPFSPLQAFAVAIANISRLFR 3086
             S+K   ++ D  Q++ F R++ + Y L+F+YPFS +QAFAVA+AN+++  +
Sbjct: 1450 ESAKNFQIELDGRQVMQFGRIDGNAYILDFQYPFSAVQAFAVALANVTQRLK 1501          
BLAST of TUB like protein 4 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000006604.1 (pep scaffold:Pmarinus_7.0:GL480314:40:7316:-1 gene:ENSPMAG00000005966.1 transcript:ENSPMAT00000006604.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 96.2857 bits (238), Expect = 5.460e-22
Identity = 62/168 (36.90%), Postives = 80/168 (47.62%), Query Frame = 3
Query:  123 TMILELESHCEHFSGSDILSTSWMQDHTHSNI------------GWLACGNKTGSVSITKTMIESDDSTESIPYELPARTNYNLR--NSKVPINIVEWNPHHKKLATCSNTGSILVYHNVNNQWSIDLVNERAHAVEVFKWSRSTNQVMIGYTDGLLIIGSVSGHRLW 584
             M   LE     +S S++L  SW     HS              GWLA GN  G V +T     S       P   P R N+NLR  NS+    +V WN  ++KLATC   G I V+     +WS++LVN+R   V  F WS    Q +I Y DG +++GSVSG R W
Sbjct:    1 AMFAALEQSPVLYSDSNLLCASWKGRVPHSERERPVCRRRCYQEGWLALGNAHGVVGVTFC---SSHCRRERP--TPQRVNFNLRGHNSEELKILVVWNEPYQKLATCDADGGIFVWILYEGRWSVELVNDRGAQVSDFTWSHDGTQALISYRDGFVLVGSVSGQRHW 163          
BLAST of TUB like protein 4 vs. Ensembl Sea Lamprey
Match: wdr35 (WD repeat domain 35 [Source:ZFIN;Acc:ZDB-GENE-060810-148])

HSP 1 Score: 67.0106 bits (162), Expect = 1.151e-11
Identity = 37/130 (28.46%), Postives = 68/130 (52.31%), Query Frame = 3
Query:  216 IGWLACGNKTGSVSITKTMIESDDSTESIPYELPARTNYNLRNS----KVPINIVEWNPHHKKLATCSNTGSILVYHNVNNQWSIDLVNERAHA-VEVFKWSRSTNQVMIGYTDGLLIIGSVSGHRLWHR 590
            +G++ACG + G + I K  +ES    ++    L A +N +L  +       + ++ WN H++KL +    G I+V+      W  +++N R  + V    W+    ++ I Y DG +I+GSV G+R+W +
Sbjct:   26 LGYIACGGEDGLLKILK--LESQTGEDAKLKGLAAPSNLSLNQTMEGHSGAVQVLAWNEHYQKLTSSDQNGLIIVWMLYKGLWYEEMINNRNKSMVRSMCWNLDGQKICIVYEDGAVIVGSVDGNRIWGK 153          
BLAST of TUB like protein 4 vs. Ensembl Nematostella
Match: EDO46375 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RQI9])

HSP 1 Score: 67.781 bits (164), Expect = 2.041e-11
Identity = 38/131 (29.01%), Postives = 66/131 (50.38%), Query Frame = 3
Query:  219 GWLACGNKTGSVSITKTMIESDDSTESIPYELPARTNYN----LRNSKVPINIVEWNPHHKKLATCSNTGSILVYHNVNNQWSIDLVNERAHAV-EVFKWSRSTNQVMIGYTDGLLIIGSVSGHRLWHRMM 596
            G++ACG + G + + K  +E+    +S    L A +N +    L      + +V WN   +KL T    G I+V+      W  +++N R  +V    KW+    ++ I Y DG +I+GSV G+R+W + +
Sbjct:   27 GYIACGGEEGLLKVLK--LETQQGKDSKLKGLAAPSNLSMNQTLEGHTGAVQVVTWNEQFQKLTTSDQYGLIIVWILYKGSWYEEMINNRNRSVVRDMKWNADGQKICIVYEDGAVIVGSVDGNRIWGKEL 155          
BLAST of TUB like protein 4 vs. Ensembl Medaka
Match: tulp4a (TUB like protein 4 [Source:NCBI gene;Acc:101163129])

HSP 1 Score: 142.124 bits (357), Expect = 9.357e-34
Identity = 104/359 (28.97%), Postives = 169/359 (47.08%), Query Frame = 3
Query: 1011 LTCGTWSPNDATIIVGTSIGSLLSFPYAWEXXXXXXXXXXXXXXXXNDSILQVEWSCPPFRRNENGGESITNQDQKYLINSNMKYPHNSFPDSEIPLNDSAASSDLENQLFDNSENFLLAVAFSSGYIYLLRHLEDVDPIKHQTGLSRLCINWNSTGTILAVGGFVRSA-NLECQCS-------VKLLSTSAEIIYFVPVPSMNKPIMSVTWGHEDTRLFVSASICVHTFRVSSSFSSLTQRCCHIIRNSLKFRQESVDKLCLPSYLHKKIHNSFRKTIQGSIPLPFRLKEFVLLPKQ--DVLHCIFQKVSTGN--GLTKYILYLEFLGASIPLLTASRSRSVKYEYHIYDPSLNQVVD 2051
            +TCG W+P+D  ++ GT+ G ++                   LL  +D I+ + W+ P F   E+  ES T+ +                 D   P +  A  S      F ++   LL V+F+SG + L+ + +D+ P   +TGL  + + W S G +LAV G  R+  + E  CS       VK  +   E IY +  P+  +PI ++ WGH D+RLF+++   ++  RV    +SL   C   I  ++K  ++ V KL +PS L   +  +F  TI+  IP P  +++FV  P    + LHC  ++       G   Y LYLE+LG  +P+L   R   ++ E+ I DP  +   D
Sbjct:  170 ITCGIWTPDDQQVLFGTADGQVIVM-------DCHGRMLAHILLHESDGIVNMSWNYPSFLV-EDSSESDTDTE-----------------DYSPPQDGRAGFS------FIHTHKPLLTVSFTSGDLSLMNNYDDLSPTLIRTGLKDVVVQWCSQGDLLAVAGMERTPLSPEAACSLPVRNATVKFYNVQGEHIYTLDTPA-QRPITTLCWGHRDSRLFLASGPALYVVRVEHRIASLQLLCQQAIALAVK-EEKDVAKLTMPSRLCSYVTAAFVPTIKPPIPDPNNMRDFVSYPTAGNERLHCTMKRTEDNPEVGGPCYTLYLEYLGGLVPILKGRRISKLRPEFVIMDPKTDGKTD 495          

HSP 2 Score: 102.064 bits (253), Expect = 2.215e-21
Identity = 51/132 (38.64%), Postives = 71/132 (53.79%), Query Frame = 3
Query:  219 GWLACGNKTGSVSITKTMIESDDSTESIPYELPARTNYNLRNSKVPINIVEWNPHHKKLATCSNTGSILVYHNVNNQWSIDLVNERAHAVEVFKWSRSTNQVMIGYTDGLLIIGSVSGHRLWHRMMDMHSAL 614
            GWLA GN  G V +T T   S    +  P +   R N+NLR     + +V WN   +KLATC   G I V+     +WS++LVN+R   V  F WS    Q +I Y DG +++GSVSG R W   +++ S +
Sbjct:   44 GWLATGNSRGVVGVTFT--SSHCRRDRPPLQ---RVNFNLRGHNSEVVLVRWNEPFQKLATCDTDGGIFVWIQYEGRWSVELVNDRGAQVSDFTWSHDGTQALISYRDGFVLVGSVSGQRHWSSEINLESQI 170          

HSP 3 Score: 53.9138 bits (128), Expect = 1.187e-6
Identity = 20/52 (38.46%), Postives = 37/52 (71.15%), Query Frame = 3
Query: 2931 HSSKRIILKYDNDQILDFSRLENDKYSLEFKYPFSPLQAFAVAIANISRLFR 3086
             S+K   ++ +  Q++ F R++ + Y L+F+YPFS +QAFAVA+AN+++  +
Sbjct: 1472 ESAKNFQIELEGRQVMQFGRIDGNAYILDFQYPFSAVQAFAVALANVTQRLK 1523          
BLAST of TUB like protein 4 vs. Ensembl Medaka
Match: tulp4a (TUB like protein 4 [Source:NCBI gene;Acc:101163129])

HSP 1 Score: 136.346 bits (342), Expect = 6.477e-32
Identity = 128/512 (25.00%), Postives = 224/512 (43.75%), Query Frame = 3
Query: 1011 LTCGTWSPNDATIIVGTSIGSLLSFPYAWEXXXXXXXXXXXXXXXXNDSILQVEWSCPPFRRNENGGESITNQDQKYLINSNMKYPHNSFPDSEIPLNDSAASSDLENQLFDNSENFLLAVAFSSGYIYLLRHLEDVDPIKHQTGLSRLCINWNSTGTILAVGGFVRSA-NLECQCS-------VKLLSTSAEIIYFVPVPSMNKPIMSVTWGHEDTRLFVSASICVHTFRVSSSFSSLTQRCCHIIRNSLKFRQESVDKLCLPSYLHKKIHNSFRKTIQGSIPLPFRLKEFVLLPKQ--DVLHCIFQKVSTGN--GLTKYILYLEFLGASIPLLTASRSRSVKYEYHIYDPSLNQVVDSDPLFPNEEME--VEHSNCTSQC------VWQRKFKHQNRRKTQNCGRNSISVLRDTDSNFSQFLRQESGKIDNCIDSKRSRLNELMTN-----DDLPENCLLAKLKSNVTLTKFELTSCQICLPRILSRINFGVN-IKLNPSNGVSVHLVEI 2468
            +TCG W+P+D  ++ GT+ G ++                   LL  +D I+ + W+ P F   E+  ES T+ +       +   P                               LL V+F+SG + L+ + +D+ P   +TGL  + + W S G +LAV G  R+  + E  CS       VK  +   E IY +  P+  +PI ++ WGH D+RLF+++   ++  RV    +SL   C   I  ++K  ++ V KL +PS L   +  +F  TI+  IP P  +++FV  P    + LHC  ++       G   Y LYLE+LG  +P+L   R   ++ E+ I DP  +   D   ++ N  +   ++  NC+          W  K   +  R +++     I++        S+   QE  +       K S  +  ++      DD+ +   LA++ SN+  TKF++      LP  L  + +  + + L P   ++++L E+
Sbjct:  170 ITCGIWTPDDQQVLFGTADGQVIVM-------DCHGRMLAHILLHESDGIVNMSWNYPSFLV-EDSSESDTDTEDYSPPQVHTHKP-------------------------------LLTVSFTSGDLSLMNNYDDLSPTLIRTGLKDVVVQWCSQGDLLAVAGMERTPLSPEAACSLPVRNATVKFYNVQGEHIYTLDTPA-QRPITTLCWGHRDSRLFLASGPALYVVRVEHRIASLQLLCQQAIALAVK-EEKDVAKLTMPSRLCSYVTAAFVPTIKPPIPDPNNMRDFVSYPTAGNERLHCTMKRTEDNPEVGGPCYTLYLEYLGGLVPILKGRRISKLRPEFVIMDPKTDGKTDE--IYGNSLISAMIDSCNCSDSSDIELNDDWVGKKSPKISRGSKSPKLPRINIDPRKSPKLSR-ATQEVSRSPRLPIRKPSIGSPSLSRKEFPLDDINQQNYLAQVTSNIWGTKFKIVGLATFLPTNLGAVIYKTSLLHLQPRQ-MTIYLPEV 636          

HSP 2 Score: 101.679 bits (252), Expect = 2.527e-21
Identity = 51/132 (38.64%), Postives = 71/132 (53.79%), Query Frame = 3
Query:  219 GWLACGNKTGSVSITKTMIESDDSTESIPYELPARTNYNLRNSKVPINIVEWNPHHKKLATCSNTGSILVYHNVNNQWSIDLVNERAHAVEVFKWSRSTNQVMIGYTDGLLIIGSVSGHRLWHRMMDMHSAL 614
            GWLA GN  G V +T T   S    +  P +   R N+NLR     + +V WN   +KLATC   G I V+     +WS++LVN+R   V  F WS    Q +I Y DG +++GSVSG R W   +++ S +
Sbjct:   44 GWLATGNSRGVVGVTFT--SSHCRRDRPPLQ---RVNFNLRGHNSEVVLVRWNEPFQKLATCDTDGGIFVWIQYEGRWSVELVNDRGAQVSDFTWSHDGTQALISYRDGFVLVGSVSGQRHWSSEINLESQI 170          

HSP 3 Score: 53.9138 bits (128), Expect = 1.097e-6
Identity = 20/52 (38.46%), Postives = 37/52 (71.15%), Query Frame = 3
Query: 2931 HSSKRIILKYDNDQILDFSRLENDKYSLEFKYPFSPLQAFAVAIANISRLFR 3086
             S+K   ++ +  Q++ F R++ + Y L+F+YPFS +QAFAVA+AN+++  +
Sbjct: 1722 ESAKNFQIELEGRQVMQFGRIDGNAYILDFQYPFSAVQAFAVALANVTQRLK 1773          
BLAST of TUB like protein 4 vs. Ensembl Medaka
Match: tulp4a (TUB like protein 4 [Source:NCBI gene;Acc:101163129])

HSP 1 Score: 136.346 bits (342), Expect = 6.477e-32
Identity = 128/512 (25.00%), Postives = 224/512 (43.75%), Query Frame = 3
Query: 1011 LTCGTWSPNDATIIVGTSIGSLLSFPYAWEXXXXXXXXXXXXXXXXNDSILQVEWSCPPFRRNENGGESITNQDQKYLINSNMKYPHNSFPDSEIPLNDSAASSDLENQLFDNSENFLLAVAFSSGYIYLLRHLEDVDPIKHQTGLSRLCINWNSTGTILAVGGFVRSA-NLECQCS-------VKLLSTSAEIIYFVPVPSMNKPIMSVTWGHEDTRLFVSASICVHTFRVSSSFSSLTQRCCHIIRNSLKFRQESVDKLCLPSYLHKKIHNSFRKTIQGSIPLPFRLKEFVLLPKQ--DVLHCIFQKVSTGN--GLTKYILYLEFLGASIPLLTASRSRSVKYEYHIYDPSLNQVVDSDPLFPNEEME--VEHSNCTSQC------VWQRKFKHQNRRKTQNCGRNSISVLRDTDSNFSQFLRQESGKIDNCIDSKRSRLNELMTN-----DDLPENCLLAKLKSNVTLTKFELTSCQICLPRILSRINFGVN-IKLNPSNGVSVHLVEI 2468
            +TCG W+P+D  ++ GT+ G ++                   LL  +D I+ + W+ P F   E+  ES T+ +       +   P                               LL V+F+SG + L+ + +D+ P   +TGL  + + W S G +LAV G  R+  + E  CS       VK  +   E IY +  P+  +PI ++ WGH D+RLF+++   ++  RV    +SL   C   I  ++K  ++ V KL +PS L   +  +F  TI+  IP P  +++FV  P    + LHC  ++       G   Y LYLE+LG  +P+L   R   ++ E+ I DP  +   D   ++ N  +   ++  NC+          W  K   +  R +++     I++        S+   QE  +       K S  +  ++      DD+ +   LA++ SN+  TKF++      LP  L  + +  + + L P   ++++L E+
Sbjct:  170 ITCGIWTPDDQQVLFGTADGQVIVM-------DCHGRMLAHILLHESDGIVNMSWNYPSFLV-EDSSESDTDTEDYSPPQVHTHKP-------------------------------LLTVSFTSGDLSLMNNYDDLSPTLIRTGLKDVVVQWCSQGDLLAVAGMERTPLSPEAACSLPVRNATVKFYNVQGEHIYTLDTPA-QRPITTLCWGHRDSRLFLASGPALYVVRVEHRIASLQLLCQQAIALAVK-EEKDVAKLTMPSRLCSYVTAAFVPTIKPPIPDPNNMRDFVSYPTAGNERLHCTMKRTEDNPEVGGPCYTLYLEYLGGLVPILKGRRISKLRPEFVIMDPKTDGKTDE--IYGNSLISAMIDSCNCSDSSDIELNDDWVGKKSPKISRGSKSPKLPRINIDPRKSPKLSR-ATQEVSRSPRLPIRKPSIGSPSLSRKEFPLDDINQQNYLAQVTSNIWGTKFKIVGLATFLPTNLGAVIYKTSLLHLQPRQ-MTIYLPEV 636          

HSP 2 Score: 101.679 bits (252), Expect = 2.527e-21
Identity = 51/132 (38.64%), Postives = 71/132 (53.79%), Query Frame = 3
Query:  219 GWLACGNKTGSVSITKTMIESDDSTESIPYELPARTNYNLRNSKVPINIVEWNPHHKKLATCSNTGSILVYHNVNNQWSIDLVNERAHAVEVFKWSRSTNQVMIGYTDGLLIIGSVSGHRLWHRMMDMHSAL 614
            GWLA GN  G V +T T   S    +  P +   R N+NLR     + +V WN   +KLATC   G I V+     +WS++LVN+R   V  F WS    Q +I Y DG +++GSVSG R W   +++ S +
Sbjct:   44 GWLATGNSRGVVGVTFT--SSHCRRDRPPLQ---RVNFNLRGHNSEVVLVRWNEPFQKLATCDTDGGIFVWIQYEGRWSVELVNDRGAQVSDFTWSHDGTQALISYRDGFVLVGSVSGQRHWSSEINLESQI 170          

HSP 3 Score: 53.9138 bits (128), Expect = 1.097e-6
Identity = 20/52 (38.46%), Postives = 37/52 (71.15%), Query Frame = 3
Query: 2931 HSSKRIILKYDNDQILDFSRLENDKYSLEFKYPFSPLQAFAVAIANISRLFR 3086
             S+K   ++ +  Q++ F R++ + Y L+F+YPFS +QAFAVA+AN+++  +
Sbjct: 1722 ESAKNFQIELEGRQVMQFGRIDGNAYILDFQYPFSAVQAFAVALANVTQRLK 1773          
BLAST of TUB like protein 4 vs. Ensembl Medaka
Match: tulp4b (tubby-related protein 4 [Source:NCBI gene;Acc:101159636])

HSP 1 Score: 132.109 bits (331), Expect = 1.394e-30
Identity = 101/352 (28.69%), Postives = 160/352 (45.45%), Query Frame = 3
Query: 1011 LTCGTWSPNDATIIVGTSIGSLLSFPYAWEXXXXXXXXXXXXXXXXNDSILQVEWSCPPFRRNENGGESITNQDQKYLINSNMKYPHNSFPDSEIPLNDSAASSDLENQLFDNSENFLLAVAFSSGYIYLLRHLEDVDPIKHQTGLSRLCINWNSTGTILAVGGFVR--------SANLECQCSVKLLSTSAEIIYFVPVPSMNKPIMSVTWGHEDTRLFVSASICVHTFRVSSSFSSLTQRCCHIIRNSLKFRQESVDKLCLPSYLHKKIHNSFRKTIQGSIPLPFRLKEFVLLPKQ--DVLHCIFQKV--STGNGLTKYILYLEFLGASIPLLTASRSRSVKYEYHIYDP 2030
            +TCG W+P+D  ++ GT+ G ++                   LL  +D I+ + W+CP F       E  T  D                PD+E            ++ L       LL V F SG I L+ + +D+ P   ++GL ++   W S G +LAV G  R         A++     VK  +   E IY +  P+  +PI ++ WGH D+RLF++    ++  RV    +SL   C   I ++L+  ++ V KL +PS L   +  +F  TI+  IP P  +++FV  P    + LHC  ++   S   G   Y LYLE+LG  +P+L   R   ++ E+ I DP
Sbjct:  177 ITCGIWTPDDQQVLFGTADGQVIVM-------DCHGRMLAHVLLHESDGIVSMSWNCPDFLL-----EDSTESD----------------PDTE------------DHVLPQRRVKPLLTVTFLSGDISLMNNYDDLSPTIIRSGL-KVEAQWCSQGDLLAVAGMERHRSPSDSACASITRNALVKFYNVQGEHIYTLETPA-QRPITTICWGHRDSRLFLACGPALYVVRVEHRVASLQLLCQQGIASALR-EEKDVGKLNMPSLLCSYVTTAFIPTIKPPIPDPNNIRDFVSYPTAGNERLHCTMKRAEESPEAGGPCYTLYLEYLGGLVPILKGRRISKLRPEFIIMDP 485          

HSP 2 Score: 100.908 bits (250), Expect = 4.362e-21
Identity = 55/136 (40.44%), Postives = 74/136 (54.41%), Query Frame = 3
Query:  219 GWLACGNKTGSVSITKTM--IESDDSTESIPYELPARTNYNLR--NSKVPINIVEWNPHHKKLATCSNTGSILVYHNVNNQWSIDLVNERAHAVEVFKWSRSTNQVMIGYTDGLLIIGSVSGHRLWHRMMDMHSAL 614
            GWLA GN  G V +T T      D ST       P R N+NLR  NS+  + +V WN   +KLATC   G I V+     +WS++LVN+R   V  F WS    Q +I Y DG +++GSVSG R W   +++ S +
Sbjct:   50 GWLATGNGRGVVGVTFTSSHCRRDRST-------PQRINFNLRGHNSEQ-VVLVRWNEPFQKLATCDMEGGIFVWIQYEGRWSVELVNDRGAQVSDFTWSHDGTQALIAYRDGFVLVGSVSGQRHWSSEINLESQI 177          

HSP 3 Score: 51.6026 bits (122), Expect = 5.895e-6
Identity = 21/53 (39.62%), Postives = 37/53 (69.81%), Query Frame = 3
Query: 2931 HSSKRIILKYDN-DQILDFSRLENDKYSLEFKYPFSPLQAFAVAIANISRLFR 3086
             S+K   ++ D   Q++ F R++ + Y L+F+YPFS +QAFAVA+AN+++  +
Sbjct: 1607 ESAKNFQIELDGRQQVMQFGRIDGNAYILDFQYPFSAVQAFAVALANVTQRLK 1659          
BLAST of TUB like protein 4 vs. Ensembl Medaka
Match: wdr35 (WD repeat domain 35 [Source:NCBI gene;Acc:101161686])

HSP 1 Score: 70.4774 bits (171), Expect = 9.256e-12
Identity = 41/142 (28.87%), Postives = 66/142 (46.48%), Query Frame = 3
Query:  183 TSWMQDHTHSNIGWLACGNKTGSVSITKTMIESDD---STESIPYELPARTNYNLRNSKVPINIVEWNPHHKKLATCSNTGSILVYHNVNNQWSIDLVNER-AHAVEVFKWSRSTNQVMIGYTDGLLIIGSVSGHRLWHRMM 596
             SW +D      G +ACG   G + + K   +SDD      + P  L    N  L   +  + +V WN  ++KL T    G I+V+      W  +++N R    V    W+    ++ I Y DG +I+GSV G+R+W + +
Sbjct:   20 VSWNKDQ-----GCIACGGDDGLLKVLKLETQSDDVRFKGLAAPSNLS--MNQTLEGHRGAVQVVTWNEQYEKLTTSDQNGLIIVWMLYKGAWYEEMINNRNKSVVRSMSWNADGQKICIVYEDGAVIVGSVDGNRIWGKEL 154          
BLAST of TUB like protein 4 vs. Planmine SMEST
Match: SMESG000001313.1 (SMESG000001313.1)

HSP 1 Score: 1935.23 bits (5012), Expect = 0.000e+0
Identity = 983/987 (99.59%), Postives = 985/987 (99.80%), Query Frame = 3
Query:  126 MILELESHCEHFSGSDILSTSWMQDHTHSNIGWLACGNKTGSVSITKTMIESDDSTESIPYELPARTNYNLRNSKVPINIVEWNPHHKKLATCSNTGSILVYHNVNNQWSIDLVNERAHAVEVFKWSRSTNQVMIGYTDGLLIIGSVSGHRLWHRMMDMHSALNQNTSYNVPTRPPNQHPLPRTDNDTXXXXXXXXXXXXXXXXXDTLMVSRNYRRTSDISTFYHADSMNRSRYFNTHQAYRPVIDESYLRMENVSPLNNGSSFVNQSLLNRHNSTQRPTLNDQSTSSHFEDMPTLTCGTWSPNDATIIVGTSIGSLLSFPYAWEXXXXXXXXXXXXXXXXNDSILQVEWSCPPFRRNENGGESITNQDQKYLINSNMKYPHNSFPDSEIPLNDSAASSDLENQLFDNSENFLLAVAFSSGYIYLLRHLEDVDPIKHQTGLSRLCINWNSTGTILAVGGFVRSANLECQCSVKLLSTSAEIIYFVPVPSMNKPIMSVTWGHEDTRLFVSASICVHTFRVSSSFSSLTQRCCHIIRNSLKFRQESVDKLCLPSYLHKKIHNSFRKTIQGSIPLPFRLKEFVLLPKQDVLHCIFQKVSTGNGLTKYILYLEFLGASIPLLTASRSRSVKYEYHIYDPSLNQVVDSDPLFPNEEMEVEHSNCTSQCVWQRKFKHQNRRKTQNCGRNSISVLRDTDSNFSQFLRQESGKIDNCIDSKRSRLNELMTNDDLPENCLLAKLKSNVTLTKFELTSCQICLPRILSRINFGVNIKLNPSNGVSVHLVEISNSTGQKMTNYYRDICLTVQGLSVSCYAENAFQSDIQFYSYSRFSNSKRSIDCSDNTKNGYPQLSDNDPNXXXXXXXXXPTHWCYIKQTINRSPLSRIKSLPSTPKKSVFNVSKGMRLPHFQNNFKNSQTPPIPSFRTFFVSNEQKDSKHDRYLHSSKRIILKYDNDQILDFSRLENDKYSLEFKYPFSPLQAFAVAIANISRLFR 3086
            MILELESHCEHFSGSDILSTSWMQDHTHSNIGWLACGNKTGSVSITKTMIESDDSTESIPYELPARTNYNLRNSKVPINIVEWNPHHKKLATCSNTGSILVYHNVNNQWSIDLVNERAHAVEVFKWSRSTNQVMIGYTDGLLIIGSVSGHRLWHRMMDMHSALNQNTSYNVPTRPPNQHPLPRTDNDTENSNLNLFENFLRNNSEDTLMVSRNYRRTSDISTFYHADSMNRSRYFNTHQAYRPVIDESYLRMENVSPLNNGSSFVNQSLLNRHNSTQRPTLNDQSTSSHFEDMPTLTCGTWSPNDATIIVGTSIGSLLSFPYAWERLPLRLVRQRDRLLRLNDSILQVEWSCPPFRRNENGGESITNQDQKYLINSNMKYPHNSFPDSEIPLNDSA SSDLENQLFDNSENFLLAVAFSSGYIYLLRHLEDVDPIKHQTGLSRLCINWNSTGTILAVGGFVRSANLECQCSVKLLSTSAEIIYFVPVPSMNKPIMSVTWGHEDTRLFVSASICVHTFRVSSSFSSLTQRCCHIIRNSLKFRQESVDKLCLPSYLHKKIHNSFRKTIQGSIPLPFRLKEFVLLPKQDVLHCIFQKVSTGNGLTKYILYLEFLGASIPLLTASRSRSVKYEYHIYDPSLNQVVDSDPLFPNEEMEVEHSNCTSQCVWQRKFKHQNRRKTQNCGRNSISVLRDTDSNFSQFLRQESGKIDNCIDSKRSRLNELMTNDDLPENCLLAKLKSNVTLTKFELTSCQICLPRILSRINFGVNIKLNPSNGVSVHLVEISNSTGQKMTNYYRDICLTVQGLSVSCYAENAFQSDIQFYSYSRFSNSKRSIDCS+NTKNGYPQLSDNDPNSASSSCYSSPTHWCYIKQTINRSPLSRIKSLPSTPKKSVFNVSKGMRLPHFQNNFKNSQTPPIPSFRTFFVSNEQ DSKHDRYLHS+KRIILKYDNDQILDFSRLENDKYSLEFKYPFSPLQAFAVAIANISRLFR
Sbjct:    1 MILELESHCEHFSGSDILSTSWMQDHTHSNIGWLACGNKTGSVSITKTMIESDDSTESIPYELPARTNYNLRNSKVPINIVEWNPHHKKLATCSNTGSILVYHNVNNQWSIDLVNERAHAVEVFKWSRSTNQVMIGYTDGLLIIGSVSGHRLWHRMMDMHSALNQNTSYNVPTRPPNQHPLPRTDNDTENSNLNLFENFLRNNSEDTLMVSRNYRRTSDISTFYHADSMNRSRYFNTHQAYRPVIDESYLRMENVSPLNNGSSFVNQSLLNRHNSTQRPTLNDQSTSSHFEDMPTLTCGTWSPNDATIIVGTSIGSLLSFPYAWERLPLRLVRQRDRLLRLNDSILQVEWSCPPFRRNENGGESITNQDQKYLINSNMKYPHNSFPDSEIPLNDSACSSDLENQLFDNSENFLLAVAFSSGYIYLLRHLEDVDPIKHQTGLSRLCINWNSTGTILAVGGFVRSANLECQCSVKLLSTSAEIIYFVPVPSMNKPIMSVTWGHEDTRLFVSASICVHTFRVSSSFSSLTQRCCHIIRNSLKFRQESVDKLCLPSYLHKKIHNSFRKTIQGSIPLPFRLKEFVLLPKQDVLHCIFQKVSTGNGLTKYILYLEFLGASIPLLTASRSRSVKYEYHIYDPSLNQVVDSDPLFPNEEMEVEHSNCTSQCVWQRKFKHQNRRKTQNCGRNSISVLRDTDSNFSQFLRQESGKIDNCIDSKRSRLNELMTNDDLPENCLLAKLKSNVTLTKFELTSCQICLPRILSRINFGVNIKLNPSNGVSVHLVEISNSTGQKMTNYYRDICLTVQGLSVSCYAENAFQSDIQFYSYSRFSNSKRSIDCSNNTKNGYPQLSDNDPNSASSSCYSSPTHWCYIKQTINRSPLSRIKSLPSTPKKSVFNVSKGMRLPHFQNNFKNSQTPPIPSFRTFFVSNEQNDSKHDRYLHSAKRIILKYDNDQILDFSRLENDKYSLEFKYPFSPLQAFAVAIANISRLFR 987          
BLAST of TUB like protein 4 vs. Planmine SMEST
Match: SMESG000066519.1 (SMESG000066519.1)

HSP 1 Score: 72.0182 bits (175), Expect = 2.311e-12
Identity = 38/130 (29.23%), Postives = 64/130 (49.23%), Query Frame = 3
Query:  210 SNIGWLACGNKTGSVSITKTMIESDDSTESIPYELPARTNYNLRNSKVPINIVEWNPHHKKLATCSNTGSILVYHNVNNQWSIDLV-NERAHAVEVFKWSRSTNQVMIGYTDGLLIIGSVSGHRLWHRMM 596
            +N G++ACG   G + + K   + + +T  I        N  L   K  +  + WN    K+ +   +G I+V+    N W  +++ N  AH V   KW+    ++ I Y DG +IIGSV G+R+W + +
Sbjct:   24 TNKGYIACGGVEGLLKVLKLDPQVNGNTRGIAAPTNLTMNETLEEHKSTLTKITWNEKFDKMTSADESGVIIVWILFKNSWHQEMINNSNAHRVNGIKWNPDGEKICIAYEDGSIIIGSVDGNRIWGKTI 153          
The following BLAST results are available for this feature:
BLAST of TUB like protein 4 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
TULP42.282e-2923.53TUB like protein 4 [Source:HGNC Symbol;Acc:HGNC:15... [more]
TULP44.614e-2924.27TUB like protein 4 [Source:HGNC Symbol;Acc:HGNC:15... [more]
TULP49.042e-1933.33TUB like protein 4 [Source:HGNC Symbol;Acc:HGNC:15... [more]
WDR354.769e-1128.91WD repeat domain 35 [Source:HGNC Symbol;Acc:HGNC:2... [more]
WDR355.052e-1128.91WD repeat domain 35 [Source:HGNC Symbol;Acc:HGNC:2... [more]
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BLAST of TUB like protein 4 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 2
Match NameE-valueIdentityDescription
tub-23.801e-1834.23TUBby-related [Source:UniProtKB/TrEMBL;Acc:Q9BL53... [more]
ifta-11.210e-1227.61IntraFlagellar Transport Associated [Source:UniPr... [more]
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BLAST of TUB like protein 4 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Tusp1.151e-3026.31gene:FBgn0039530 transcript:FBtr0335246[more]
Tusp1.266e-3026.31gene:FBgn0039530 transcript:FBtr0302589[more]
Tusp1.266e-3026.31gene:FBgn0039530 transcript:FBtr0302590[more]
Tusp2.852e-2926.83gene:FBgn0039530 transcript:FBtr0335245[more]
Oseg41.527e-628.57gene:FBgn0035264 transcript:FBtr0072797[more]
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BLAST of TUB like protein 4 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
tulp4b4.847e-3325.10TUB like protein 4b [Source:ZFIN;Acc:ZDB-GENE-1305... [more]
tulp4b2.144e-3225.40TUB like protein 4b [Source:ZFIN;Acc:ZDB-GENE-1305... [more]
tulp4a3.474e-2924.77TUB like protein 4a [Source:ZFIN;Acc:ZDB-GENE-0605... [more]
tulp4a4.496e-2924.77TUB like protein 4a [Source:ZFIN;Acc:ZDB-GENE-0605... [more]
wdr356.004e-1127.74WD repeat domain 35 [Source:ZFIN;Acc:ZDB-GENE-0608... [more]
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BLAST of TUB like protein 4 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
tulp45.178e-2924.02B-cell receptor CD22-like [Source:Xenbase;Acc:XB-G... [more]
tulp41.047e-2826.74B-cell receptor CD22-like [Source:Xenbase;Acc:XB-G... [more]
tulp41.225e-2826.74B-cell receptor CD22-like [Source:Xenbase;Acc:XB-G... [more]
tulp46.594e-2725.82B-cell receptor CD22-like [Source:Xenbase;Acc:XB-G... [more]
WDR357.991e-1327.34WD repeat domain 35 [Source:NCBI gene;Acc:10049637... [more]
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BLAST of TUB like protein 4 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Tulp44.063e-3124.66tubby like protein 4 [Source:MGI Symbol;Acc:MGI:19... [more]
Tulp42.618e-2826.40tubby like protein 4 [Source:MGI Symbol;Acc:MGI:19... [more]
Wdr353.001e-1229.50WD repeat domain 35 [Source:MGI Symbol;Acc:MGI:192... [more]
Wdr353.088e-1229.50WD repeat domain 35 [Source:MGI Symbol;Acc:MGI:192... [more]
Wdr352.944e-1027.91WD repeat domain 35 [Source:MGI Symbol;Acc:MGI:192... [more]
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BLAST of TUB like protein 4 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q9JIL5|TULP4_MOUSE2.479e-3024.66Tubby-related protein 4 OS=Mus musculus OX=10090 G... [more]
sp|Q9NRJ4|TULP4_HUMAN1.950e-2824.27Tubby-related protein 4 OS=Homo sapiens OX=9606 GN... [more]
sp|Q8BND3|WDR35_MOUSE2.160e-1129.50WD repeat-containing protein 35 OS=Mus musculus OX... [more]
sp|A6N6J5|WDR35_RAT2.247e-1129.50WD repeat-containing protein 35 OS=Rattus norvegic... [more]
sp|Q9P2L0|WDR35_HUMAN2.426e-1028.91WD repeat-containing protein 35 OS=Homo sapiens OX... [more]
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BLAST of TUB like protein 4 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A210QDL87.064e-5028.63Tubby-related protein 4 OS=Mizuhopecten yessoensis... [more]
A0A2C9KB248.737e-4427.31SOCS box domain-containing protein OS=Biomphalaria... [more]
A0A2T7P6951.018e-4227.72Tub domain-containing protein OS=Pomacea canalicul... [more]
K1QME86.113e-4130.45Tubby-related protein 4 OS=Crassostrea gigas OX=29... [more]
R7T9E47.075e-3826.91Tub domain-containing protein OS=Capitella teleta ... [more]
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BLAST of TUB like protein 4 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
tulp4b2.702e-3228.13TUB like protein 4b [Source:ZFIN;Acc:ZDB-GENE-1305... [more]
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BLAST of TUB like protein 4 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 2
Match NameE-valueIdentityDescription
ENSPMAT00000006604.15.460e-2236.90pep scaffold:Pmarinus_7.0:GL480314:40:7316:-1 gene... [more]
wdr351.151e-1128.46WD repeat domain 35 [Source:ZFIN;Acc:ZDB-GENE-0608... [more]
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BLAST of TUB like protein 4 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of TUB like protein 4 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO463752.041e-1129.01Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of TUB like protein 4 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
tulp4a9.357e-3428.97TUB like protein 4 [Source:NCBI gene;Acc:101163129... [more]
tulp4a6.477e-3225.00TUB like protein 4 [Source:NCBI gene;Acc:101163129... [more]
tulp4a6.477e-3225.00TUB like protein 4 [Source:NCBI gene;Acc:101163129... [more]
tulp4b1.394e-3028.69tubby-related protein 4 [Source:NCBI gene;Acc:1011... [more]
wdr359.256e-1228.87WD repeat domain 35 [Source:NCBI gene;Acc:10116168... [more]
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BLAST of TUB like protein 4 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000001313.10.000e+099.59SMESG000001313.1[more]
SMESG000066519.12.311e-1229.23SMESG000066519.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30028792 ID=SMED30028792|Name=TUB like protein 4|organism=Schmidtea mediterranea sexual|type=transcript|length=3160bp
GCTTTTAGTAATTTAATAAGAATTAAAAATAATATCTATTTATATAATTT
ATCAACGAAACAACTGTAATTAAACTTCATTTCTTTCTAAATTTTTCATT
GAAATATAATGAAAATCTATACACGATGATTTTGGAATTAGAGAGCCATT
GTGAACATTTCAGTGGGTCAGATATTCTATCCACTTCATGGATGCAAGAC
CATACACATTCCAATATCGGTTGGCTGGCTTGTGGAAATAAGACCGGTTC
TGTAAGTATAACAAAAACAATGATAGAATCTGATGATTCGACCGAATCTA
TTCCATATGAATTACCTGCAAGAACTAACTATAATTTGCGTAACAGTAAA
GTACCAATAAATATCGTCGAATGGAATCCTCACCATAAAAAGTTAGCAAC
ATGTAGCAATACTGGTTCAATATTGGTTTATCACAATGTTAACAATCAAT
GGTCAATTGACTTGGTCAATGAAAGAGCACATGCAGTCGAAGTATTTAAG
TGGTCAAGATCTACTAATCAAGTTATGATTGGTTATACGGACGGCTTGTT
AATTATTGGTTCGGTTAGCGGGCATCGTTTATGGCATCGGATGATGGACA
TGCATAGCGCTCTTAATCAAAATACTAGCTATAACGTACCGACTCGCCCA
CCAAACCAACATCCATTACCAAGAACGGATAACGATACAGAAAACTCTAA
TCTGAATTTATTTGAAAATTTCCTGCGCAATAATTCCGAAGACACATTGA
TGGTGTCAAGAAATTACCGCCGTACTAGTGATATATCAACATTTTATCAC
GCTGATTCAATGAATAGATCTCGATACTTCAATACCCATCAAGCATATCG
CCCAGTGATTGACGAATCATATCTTAGAATGGAAAATGTTTCGCCTTTGA
ATAACGGATCAAGCTTTGTAAATCAATCTTTATTAAACCGACACAATTCC
ACTCAAAGACCAACTCTAAATGATCAATCTACTTCGTCCCATTTTGAAGA
TATGCCAACACTTACTTGTGGTACTTGGAGTCCTAATGATGCCACAATAA
TAGTTGGAACGTCTATAGGATCTCTTCTATCTTTTCCTTACGCATGGGAG
CGGCTTCCTTTAAGATTAGTTCGTCAAAGAGATCGTTTATTACGACTGAA
TGACTCTATTCTTCAAGTAGAATGGTCTTGCCCACCATTTAGACGAAATG
AAAATGGTGGAGAAAGCATTACAAATCAAGACCAGAAGTATTTAATAAAT
AGCAACATGAAATATCCCCATAACTCATTCCCAGATTCCGAGATTCCACT
GAATGATTCTGCGGCTAGTTCTGATTTGGAGAATCAACTATTTGATAATT
CTGAAAACTTTTTGCTAGCAGTTGCCTTTAGCAGTGGATATATTTATTTA
CTACGACATTTGGAAGACGTGGACCCTATCAAACATCAAACTGGTCTTTC
CCGGTTGTGTATTAATTGGAATAGTACCGGAACTATCCTTGCTGTCGGCG
GTTTTGTACGCAGTGCTAATCTAGAATGCCAATGTTCAGTTAAGCTTCTG
TCTACTTCCGCAGAAATAATTTATTTTGTCCCAGTCCCATCCATGAATAA
ACCAATAATGAGTGTTACATGGGGTCATGAAGACACAAGACTGTTCGTAT
CAGCCAGCATTTGTGTACATACTTTTCGTGTCAGTAGCAGCTTTTCTTCA
TTGACTCAAAGATGTTGTCACATTATCCGAAACAGTCTAAAGTTTCGTCA
GGAATCAGTTGATAAGCTCTGTTTGCCTTCTTATTTACACAAGAAAATCC
ACAATTCATTCCGAAAAACAATTCAGGGTTCAATACCGCTGCCTTTTCGA
TTGAAAGAATTCGTCCTATTACCCAAACAAGATGTGTTGCATTGTATTTT
TCAAAAAGTGTCCACTGGAAATGGTTTGACAAAATATATTCTCTATCTGG
AGTTTCTCGGTGCTTCAATACCATTACTGACGGCGTCCCGCAGCAGAAGC
GTCAAATATGAATATCATATTTACGACCCATCCCTCAATCAAGTTGTTGA
TTCTGATCCTTTATTTCCCAATGAAGAAATGGAAGTGGAGCACAGCAATT
GCACTAGTCAATGCGTATGGCAGCGCAAATTCAAGCATCAAAATCGCAGA
AAAACACAAAATTGTGGCAGAAATTCAATTTCTGTGTTGCGAGACACTGA
TTCGAATTTCTCGCAATTCCTCAGACAAGAATCCGGGAAAATTGACAATT
GCATAGATTCTAAACGATCTCGACTCAATGAACTGATGACGAATGACGAT
CTCCCAGAAAATTGTCTGTTAGCAAAACTAAAATCTAACGTAACTTTGAC
AAAATTTGAATTAACAAGTTGTCAAATTTGTTTACCGCGTATTTTGTCGA
GGATAAATTTTGGAGTAAATATCAAACTAAATCCGTCAAATGGGGTATCC
GTTCATTTGGTAGAGATTAGCAATTCGACCGGCCAGAAAATGACCAACTA
TTACAGAGATATTTGCCTCACTGTCCAAGGCTTATCGGTCTCTTGTTATG
CTGAAAATGCATTTCAATCAGACATTCAATTTTATTCTTATTCTCGGTTT
TCAAATTCAAAACGAAGCATTGATTGTAGCGATAATACAAAAAACGGCTA
CCCCCAATTATCCGATAATGATCCCAACTCTGCCTCATCTTCATGTTATT
CCTCACCTACTCATTGGTGCTATATCAAACAAACAATTAATCGTTCTCCA
TTGTCCCGAATCAAAAGTCTCCCTTCGACACCAAAGAAATCTGTTTTCAA
TGTCTCTAAAGGAATGCGATTGCCACACTTTCAGAACAATTTCAAAAATT
CGCAAACTCCTCCAATTCCTTCATTTCGTACATTTTTTGTTTCAAATGAA
CAAAAAGATTCAAAGCATGACCGATATTTGCATTCATCGAAAAGAATTAT
TTTAAAATATGATAATGATCAAATATTAGATTTTTCTCGCCTGGAAAATG
ACAAATATTCATTGGAATTTAAATATCCTTTCTCACCACTTCAGGCCTTT
GCGGTGGCAATAGCCAATATAAGCAGATTATTTAGATGAATGACTTGCTA
AAATGAAGATGAGATTTCTTTGACTTTTATTATTATGATGTTGATAATTA
TTGATTACTG
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protein sequence of SMED30028792-orf-1

>SMED30028792-orf-1 ID=SMED30028792-orf-1|Name=SMED30028792-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=988bp
MILELESHCEHFSGSDILSTSWMQDHTHSNIGWLACGNKTGSVSITKTMI
ESDDSTESIPYELPARTNYNLRNSKVPINIVEWNPHHKKLATCSNTGSIL
VYHNVNNQWSIDLVNERAHAVEVFKWSRSTNQVMIGYTDGLLIIGSVSGH
RLWHRMMDMHSALNQNTSYNVPTRPPNQHPLPRTDNDTENSNLNLFENFL
RNNSEDTLMVSRNYRRTSDISTFYHADSMNRSRYFNTHQAYRPVIDESYL
RMENVSPLNNGSSFVNQSLLNRHNSTQRPTLNDQSTSSHFEDMPTLTCGT
WSPNDATIIVGTSIGSLLSFPYAWERLPLRLVRQRDRLLRLNDSILQVEW
SCPPFRRNENGGESITNQDQKYLINSNMKYPHNSFPDSEIPLNDSAASSD
LENQLFDNSENFLLAVAFSSGYIYLLRHLEDVDPIKHQTGLSRLCINWNS
TGTILAVGGFVRSANLECQCSVKLLSTSAEIIYFVPVPSMNKPIMSVTWG
HEDTRLFVSASICVHTFRVSSSFSSLTQRCCHIIRNSLKFRQESVDKLCL
PSYLHKKIHNSFRKTIQGSIPLPFRLKEFVLLPKQDVLHCIFQKVSTGNG
LTKYILYLEFLGASIPLLTASRSRSVKYEYHIYDPSLNQVVDSDPLFPNE
EMEVEHSNCTSQCVWQRKFKHQNRRKTQNCGRNSISVLRDTDSNFSQFLR
QESGKIDNCIDSKRSRLNELMTNDDLPENCLLAKLKSNVTLTKFELTSCQ
ICLPRILSRINFGVNIKLNPSNGVSVHLVEISNSTGQKMTNYYRDICLTV
QGLSVSCYAENAFQSDIQFYSYSRFSNSKRSIDCSDNTKNGYPQLSDNDP
NSASSSCYSSPTHWCYIKQTINRSPLSRIKSLPSTPKKSVFNVSKGMRLP
HFQNNFKNSQTPPIPSFRTFFVSNEQKDSKHDRYLHSSKRIILKYDNDQI
LDFSRLENDKYSLEFKYPFSPLQAFAVAIANISRLFR*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
PLANA:0000418head
Vocabulary: INTERPRO
TermDefinition
IPR036322WD40_repeat_dom_sf
IPR000007Tubby_C
IPR015943WD40/YVTN_repeat-like_dom_sf
IPR025659Tubby-like_C
IPR017986WD40_repeat_dom
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
Vocabulary: cellular component
TermDefinition
GO:0005622intracellular
Vocabulary: biological process
TermDefinition
GO:0035556intracellular signal transduction
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR025659Tubby-like, C-terminalGENE3DG3DSA:3.20.90.10coord: 902..985
e-value: 2.0E-7
score: 32.5
IPR025659Tubby-like, C-terminalSUPERFAMILYSSF54518Tubby C-terminal domain-likecoord: 730..983
IPR015943WD40/YVTN repeat-like-containing domain superfamilyGENE3DG3DSA:2.130.10.10coord: 4..171
e-value: 1.3E-7
score: 33.0
IPR000007Tubby, C-terminalPFAMPF01167Tubcoord: 732..982
e-value: 1.4E-7
score: 31.5
NoneNo IPR availablePANTHERPTHR16517TUBBY-RELATEDcoord: 287..683
coord: 946..982
coord: 30..162
NoneNo IPR availablePANTHERPTHR16517:SF2TUBBY-RELATED PROTEIN 4coord: 287..683
coord: 946..982
coord: 30..162
IPR036322WD40-repeat-containing domain superfamilySUPERFAMILYSSF50978WD40 repeat-likecoord: 30..512