SMED30028734
Overview
Neoblast Clusters
Zeng et. al., 2018
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny AppEmbryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30028734 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 7Note: Hover over icons to view figure legend
Alignments
SMED30028734 aligns in the following genomic locations:
Homology
BLAST of SMED30028734 vs. Planmine SMEST
Match: SMESG000036067.1 (SMESG000036067.1) HSP 1 Score: 242.276 bits (617), Expect = 3.321e-76 Identity = 129/142 (90.85%), Postives = 136/142 (95.77%), Query Frame = 1 Query: 1 LLSEKDNLKFLIKEMDDKNKILQNNYNKSEEEN--LKFQRDKYLAEINKLESLLKESYATNRRLHKDCDDCKAELQRSEERNSQLENEIEVYNRKNKEIAKYFQMQIKDSNCSNERLKLELKQSENKFKSLQNENNNIKSGF 420 LLSE+DNLKFL+KE+DDKNKILQNNYNKSEEEN LKFQRDKYLAEINKLES+LKESY TNRRLHKDCDDCKAELQRSEERNSQLENEIEV NRKNKEIAKYFQMQIKDSN SNERLKLELKQSENKF+SLQN+NNNI+S F Sbjct: 428 LLSERDNLKFLVKEIDDKNKILQNNYNKSEEENENLKFQRDKYLAEINKLESVLKESYTTNRRLHKDCDDCKAELQRSEERNSQLENEIEVNNRKNKEIAKYFQMQIKDSNYSNERLKLELKQSENKFQSLQNDNNNIESRF 569 HSP 2 Score: 95.1301 bits (235), Expect = 1.607e-23 Identity = 63/133 (47.37%), Postives = 89/133 (66.92%), Query Frame = 1 Query: 22 LKFLIKEMDDKNKILQNNYNKSEEENLKFQRDKYLAEINKLESLLKESYATNRRLHKDCDDCKAELQRSEERNSQLENEIEVYNRKNKEIAKYFQMQIKDSNCSNERLKLELKQSENKFKSLQNENNNIKSGF 420 LK +K+ ++K + LQN+ N E ++ +I +L+S LKES L +D +DCK +L+ S+ERN QLENE+ V R +EI + FQ++++DSN SNE++ LEL +SE KFKSLQ ENNNIKSGF Sbjct: 544 LKLELKQSENKFQSLQNDNNNIE--------SRFAEQIEQLQSALKESDRKMGSLQRDFEDCKEKLKHSQERNLQLENELVVNKRTIEEITEDFQLKMEDSNSSNEKMLLELNRSEYKFKSLQIENNNIKSGF 668 HSP 3 Score: 56.6102 bits (135), Expect = 4.943e-10 Identity = 51/149 (34.23%), Postives = 80/149 (53.69%), Query Frame = 1 Query: 31 LIKEMDDKNKILQNNYNKSEEENLKFQRDKYLAEINKLESLLKESYATNRRLHKDCDDCKAELQRSEERNSQLE---------------------NEIEVYNRKNKEIAKYFQMQIKDSNCSNERLKLELKQSENKFKSLQNENNNIKS 414 LIK++ +N + Y K + EN ++YLA+I +L+S +ES TN+RL + D K +L+RS+ERN +LE N+++ K IAK + Q+ + N S + LK +L + NK K LQ+EN++IKS Sbjct: 188 LIKQLQKENSQFEGKYKKLQNEN-----NQYLAQIERLKSGFRESERTNKRLQLNIDQYKTKLERSQERNLKLESANRLKSQMERNRCVNCAELINQLQNEKGKTNRIAKDCEFQLDNLNRSVKNLKSQLNKMGNKCKDLQDENDHIKS 331
BLAST of SMED30028734 vs. Planmine SMEST
Match: SMESG000036067.1 (SMESG000036067.1) HSP 1 Score: 241.891 bits (616), Expect = 4.077e-76 Identity = 129/142 (90.85%), Postives = 136/142 (95.77%), Query Frame = 1 Query: 1 LLSEKDNLKFLIKEMDDKNKILQNNYNKSEEEN--LKFQRDKYLAEINKLESLLKESYATNRRLHKDCDDCKAELQRSEERNSQLENEIEVYNRKNKEIAKYFQMQIKDSNCSNERLKLELKQSENKFKSLQNENNNIKSGF 420 LLSE+DNLKFL+KE+DDKNKILQNNYNKSEEEN LKFQRDKYLAEINKLES+LKESY TNRRLHKDCDDCKAELQRSEERNSQLENEIEV NRKNKEIAKYFQMQIKDSN SNERLKLELKQSENKF+SLQN+NNNI+S F Sbjct: 423 LLSERDNLKFLVKEIDDKNKILQNNYNKSEEENENLKFQRDKYLAEINKLESVLKESYTTNRRLHKDCDDCKAELQRSEERNSQLENEIEVNNRKNKEIAKYFQMQIKDSNYSNERLKLELKQSENKFQSLQNDNNNIESRF 564 HSP 2 Score: 95.1301 bits (235), Expect = 1.876e-23 Identity = 63/133 (47.37%), Postives = 89/133 (66.92%), Query Frame = 1 Query: 22 LKFLIKEMDDKNKILQNNYNKSEEENLKFQRDKYLAEINKLESLLKESYATNRRLHKDCDDCKAELQRSEERNSQLENEIEVYNRKNKEIAKYFQMQIKDSNCSNERLKLELKQSENKFKSLQNENNNIKSGF 420 LK +K+ ++K + LQN+ N E ++ +I +L+S LKES L +D +DCK +L+ S+ERN QLENE+ V R +EI + FQ++++DSN SNE++ LEL +SE KFKSLQ ENNNIKSGF Sbjct: 539 LKLELKQSENKFQSLQNDNNNIE--------SRFAEQIEQLQSALKESDRKMGSLQRDFEDCKEKLKHSQERNLQLENELVVNKRTIEEITEDFQLKMEDSNSSNEKMLLELNRSEYKFKSLQIENNNIKSGF 663 HSP 3 Score: 56.225 bits (134), Expect = 5.820e-10 Identity = 51/149 (34.23%), Postives = 80/149 (53.69%), Query Frame = 1 Query: 31 LIKEMDDKNKILQNNYNKSEEENLKFQRDKYLAEINKLESLLKESYATNRRLHKDCDDCKAELQRSEERNSQLE---------------------NEIEVYNRKNKEIAKYFQMQIKDSNCSNERLKLELKQSENKFKSLQNENNNIKS 414 LIK++ +N + Y K + EN ++YLA+I +L+S +ES TN+RL + D K +L+RS+ERN +LE N+++ K IAK + Q+ + N S + LK +L + NK K LQ+EN++IKS Sbjct: 183 LIKQLQKENSQFEGKYKKLQNEN-----NQYLAQIERLKSGFRESERTNKRLQLNIDQYKTKLERSQERNLKLESANRLKSQMERNRCVNCAELINQLQNEKGKTNRIAKDCEFQLDNLNRSVKNLKSQLNKMGNKCKDLQDENDHIKS 326
BLAST of SMED30028734 vs. Planmine SMEST
Match: SMESG000036067.1 (SMESG000036067.1) HSP 1 Score: 241.506 bits (615), Expect = 1.223e-75 Identity = 129/142 (90.85%), Postives = 136/142 (95.77%), Query Frame = 1 Query: 1 LLSEKDNLKFLIKEMDDKNKILQNNYNKSEEEN--LKFQRDKYLAEINKLESLLKESYATNRRLHKDCDDCKAELQRSEERNSQLENEIEVYNRKNKEIAKYFQMQIKDSNCSNERLKLELKQSENKFKSLQNENNNIKSGF 420 LLSE+DNLKFL+KE+DDKNKILQNNYNKSEEEN LKFQRDKYLAEINKLES+LKESY TNRRLHKDCDDCKAELQRSEERNSQLENEIEV NRKNKEIAKYFQMQIKDSN SNERLKLELKQSENKF+SLQN+NNNI+S F Sbjct: 423 LLSERDNLKFLVKEIDDKNKILQNNYNKSEEENENLKFQRDKYLAEINKLESVLKESYTTNRRLHKDCDDCKAELQRSEERNSQLENEIEVNNRKNKEIAKYFQMQIKDSNYSNERLKLELKQSENKFQSLQNDNNNIESRF 564 HSP 2 Score: 94.7449 bits (234), Expect = 2.161e-23 Identity = 63/133 (47.37%), Postives = 89/133 (66.92%), Query Frame = 1 Query: 22 LKFLIKEMDDKNKILQNNYNKSEEENLKFQRDKYLAEINKLESLLKESYATNRRLHKDCDDCKAELQRSEERNSQLENEIEVYNRKNKEIAKYFQMQIKDSNCSNERLKLELKQSENKFKSLQNENNNIKSGF 420 LK +K+ ++K + LQN+ N E ++ +I +L+S LKES L +D +DCK +L+ S+ERN QLENE+ V R +EI + FQ++++DSN SNE++ LEL +SE KFKSLQ ENNNIKSGF Sbjct: 539 LKLELKQSENKFQSLQNDNNNIE--------SRFAEQIEQLQSALKESDRKMGSLQRDFEDCKEKLKHSQERNLQLENELVVNKRTIEEITEDFQLKMEDSNSSNEKMLLELNRSEYKFKSLQIENNNIKSGF 663 HSP 3 Score: 56.6102 bits (135), Expect = 4.874e-10 Identity = 51/149 (34.23%), Postives = 80/149 (53.69%), Query Frame = 1 Query: 31 LIKEMDDKNKILQNNYNKSEEENLKFQRDKYLAEINKLESLLKESYATNRRLHKDCDDCKAELQRSEERNSQLE---------------------NEIEVYNRKNKEIAKYFQMQIKDSNCSNERLKLELKQSENKFKSLQNENNNIKS 414 LIK++ +N + Y K + EN ++YLA+I +L+S +ES TN+RL + D K +L+RS+ERN +LE N+++ K IAK + Q+ + N S + LK +L + NK K LQ+EN++IKS Sbjct: 183 LIKQLQKENSQFEGKYKKLQNEN-----NQYLAQIERLKSGFRESERTNKRLQLNIDQYKTKLERSQERNLKLESANRLKSQMERNRCVNCAELINQLQNEKGKTNRIAKDCEFQLDNLNRSVKNLKSQLNKMGNKCKDLQDENDHIKS 326
BLAST of SMED30028734 vs. Planmine SMEST
Match: SMESG000036067.1 (SMESG000036067.1) HSP 1 Score: 241.506 bits (615), Expect = 1.389e-75 Identity = 129/142 (90.85%), Postives = 136/142 (95.77%), Query Frame = 1 Query: 1 LLSEKDNLKFLIKEMDDKNKILQNNYNKSEEEN--LKFQRDKYLAEINKLESLLKESYATNRRLHKDCDDCKAELQRSEERNSQLENEIEVYNRKNKEIAKYFQMQIKDSNCSNERLKLELKQSENKFKSLQNENNNIKSGF 420 LLSE+DNLKFL+KE+DDKNKILQNNYNKSEEEN LKFQRDKYLAEINKLES+LKESY TNRRLHKDCDDCKAELQRSEERNSQLENEIEV NRKNKEIAKYFQMQIKDSN SNERLKLELKQSENKF+SLQN+NNNI+S F Sbjct: 428 LLSERDNLKFLVKEIDDKNKILQNNYNKSEEENENLKFQRDKYLAEINKLESVLKESYTTNRRLHKDCDDCKAELQRSEERNSQLENEIEVNNRKNKEIAKYFQMQIKDSNYSNERLKLELKQSENKFQSLQNDNNNIESRF 569 HSP 2 Score: 94.7449 bits (234), Expect = 2.336e-23 Identity = 63/133 (47.37%), Postives = 89/133 (66.92%), Query Frame = 1 Query: 22 LKFLIKEMDDKNKILQNNYNKSEEENLKFQRDKYLAEINKLESLLKESYATNRRLHKDCDDCKAELQRSEERNSQLENEIEVYNRKNKEIAKYFQMQIKDSNCSNERLKLELKQSENKFKSLQNENNNIKSGF 420 LK +K+ ++K + LQN+ N E ++ +I +L+S LKES L +D +DCK +L+ S+ERN QLENE+ V R +EI + FQ++++DSN SNE++ LEL +SE KFKSLQ ENNNIKSGF Sbjct: 544 LKLELKQSENKFQSLQNDNNNIE--------SRFAEQIEQLQSALKESDRKMGSLQRDFEDCKEKLKHSQERNLQLENELVVNKRTIEEITEDFQLKMEDSNSSNEKMLLELNRSEYKFKSLQIENNNIKSGF 668 HSP 3 Score: 56.6102 bits (135), Expect = 5.167e-10 Identity = 51/149 (34.23%), Postives = 80/149 (53.69%), Query Frame = 1 Query: 31 LIKEMDDKNKILQNNYNKSEEENLKFQRDKYLAEINKLESLLKESYATNRRLHKDCDDCKAELQRSEERNSQLE---------------------NEIEVYNRKNKEIAKYFQMQIKDSNCSNERLKLELKQSENKFKSLQNENNNIKS 414 LIK++ +N + Y K + EN ++YLA+I +L+S +ES TN+RL + D K +L+RS+ERN +LE N+++ K IAK + Q+ + N S + LK +L + NK K LQ+EN++IKS Sbjct: 188 LIKQLQKENSQFEGKYKKLQNEN-----NQYLAQIERLKSGFRESERTNKRLQLNIDQYKTKLERSQERNLKLESANRLKSQMERNRCVNCAELINQLQNEKGKTNRIAKDCEFQLDNLNRSVKNLKSQLNKMGNKCKDLQDENDHIKS 331
BLAST of SMED30028734 vs. Planmine SMEST
Match: SMESG000047799.1 (SMESG000047799.1) HSP 1 Score: 176.407 bits (446), Expect = 1.184e-52 Identity = 94/121 (77.69%), Postives = 108/121 (89.26%), Query Frame = 1 Query: 1 LLSEKDNLKFLIKEMDDKNKILQNNYNKSEEEN--LKFQRDKYLAEINKLESLLKESYATNRRLHKDCDDCKAELQRSEERNSQLENEIEVYNRKNKEIAKYFQMQIKDSNCSNERLKLEL 357 LLSE+DNLKFL+KE+DDKNKILQNNYNKS EEN LK QRDKYLAEINKLES+LKESY TNRRLHKDCDDCKAELQRSEERNSQ +NEI+V RK +EI K +Q+++++SN S E+LKLEL Sbjct: 381 LLSERDNLKFLVKEIDDKNKILQNNYNKSGEENENLKIQRDKYLAEINKLESVLKESYTTNRRLHKDCDDCKAELQRSEERNSQFKNEIDVNKRKIEEITKDYQLKMENSNNSIEKLKLEL 501 HSP 2 Score: 83.5741 bits (205), Expect = 2.100e-19 Identity = 58/132 (43.94%), Postives = 81/132 (61.36%), Query Frame = 1 Query: 82 KSEEENLKFQRDKYLAEINKLESLLKESYATNRRLHKDCDDCKAELQRSEERNSQLENEI-------------------EVYNRKNKEIAKYFQMQIKDSNCSNERLKLELKQSENKFKSLQNENNNIKSGF 420 K++ E K + + + +IN+LES LKE N RL +D DDCK +L +S+ RNSQLE+EI + RK +EI+K FQ+++ +S SNE+LKLEL Q +NK KSLQN+N+N KS F Sbjct: 201 KTQLEKKKTRVKRQIEKINQLESELKELEGINLRLQQDFDDCKVKLNQSQRRNSQLESEINKKQCENCAELIKQLQKQKDTSKRKTEEISKDFQLKMDNSKTSNEKLKLELNQLKNKCKSLQNQNDNDKSQF 332 HSP 3 Score: 67.0106 bits (162), Expect = 1.333e-13 Identity = 37/59 (62.71%), Postives = 45/59 (76.27%), Query Frame = 1 Query: 139 KLESLLKESYATNRRLHKDCDDCKAELQRSEERNSQLENEIEVYNRKNKEIAKYFQMQI 315 +LES LKES +LHKD DDCKAELQRS ERN QLENE+E+ RK +EI K FQ+++ Sbjct: 593 QLESELKESDKIKNKLHKDYDDCKAELQRSAERNLQLENEMEINKRKIEEIKKDFQLKL 651 The following BLAST results are available for this feature:
BLAST of SMED30028734 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30028734 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30028734 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30028734 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30028734 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30028734 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30028734 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30028734 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30028734 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30028734 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30028734 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30028734 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30028734 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30028734 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30028734 ID=SMED30028734|Name=SMED30028734|organism=Schmidtea mediterranea sexual|type=transcript|length=420bpback to top protein sequence of SMED30028734-orf-1 >SMED30028734-orf-1 ID=SMED30028734-orf-1|Name=SMED30028734-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=140bp LLSEKDNLKFLIKEMDDKNKILQNNYNKSEEENLKFQRDKYLAEINKLESback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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