SMED30028732

Overview
NameSMED30028732
Smed IDSMED30028732
Length (bp)1265
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30028732 (SMED30028732) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30028732 (SMED30028732) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30028732 (SMED30028732) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30028732 (SMED30028732) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30028732

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30028732SMESG000022822.1 dd_Smed_v4_3932_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30028732SMESG000022822.1 dd_Smed_v4_3932_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30028732SMESG000022822.1 dd_Smed_v4_3932_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
epidermisSMED30028732SMESG000022822.1 SMESG000022815.1 dd_Smed_v4_3700_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30028732 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.002110:64848..66111 +6221
v31.001816:13112..15129 +5195
v31.002110:64471..65549 +1267
Homology
[Close]
BLAST of SMED30028732 vs. Planmine SMEST
Match: SMESG000022822.1 (SMESG000022822.1)

HSP 1 Score: 544.273 bits (1401), Expect = 0.000e+0
Identity = 297/301 (98.67%), Postives = 299/301 (99.34%), Query Frame = 2 
Query:    2 DPITKLVKSYAGGEMKEIWICSKCGNENRKENDKCDRKNCYQSKLNARSKEITNHKEIITNQKEELQQNGNEIILNLNQWRCEKCTLINKNNIFQCSACNWERKLDCWQCWNGDCNAVNKGVNMFCQKCKLDRNPPDPKTKLVKSYAGGEEKEIWIXXXXXXXXXXXXXXXAGNNCHTFKFYDGTNSTNKDNDRVTVTLHNPNYGKGQKAXXXXXXXXXXXXSWKCWFGNCKQLNVAGEEFCKNCRLHRYGTPDPGALVKVRNKNGDVDDMWICHKCFKYNELTDAQCRGKSCKLGRFDYV 904
            DPITKLVKSYAG E+KEIWIC KCGNENRKENDKCD+KNCYQSKLNARSKEITNHKEIITNQKEELQQNGNEIILNLNQWRCEKCTLINKNNIFQCSACNWERKLDCWQCWNGDCNAVNKGVNMFCQKCKLDRNPPDPKTKLVKSYAGGEEKEIWICSKCKKKNKKEDAKCAGNNCHTFKFYDGTNSTNKDNDRVTVTLHNPNYGKGQKANNINNENLSNGNSWKCWFGNCKQLNVAGEEFCKNCRLHRYGTPDPGALVKVRNKNGDVDDMWICHKCFKYNELTDAQCRGKSCKLGRFDYV
Sbjct:  470 DPITKLVKSYAGKEIKEIWICEKCGNENRKENDKCDKKNCYQSKLNARSKEITNHKEIITNQKEELQQNGNEIILNLNQWRCEKCTLINKNNIFQCSACNWERKLDCWQCWNGDCNAVNKGVNMFCQKCKLDRNPPDPKTKLVKSYAGGEEKEIWICSKCKKKNKKEDAKCAGNNCHTFKFYDGTNSTNKDNDRVTVTLHNPNYGKGQKANNINNENLSNGNSWKCWFGNCKQLNVAGEEFCKNCRLHRYGTPDPGALVKVRNKNGDVDDMWICHKCFKYNELTDAQCRGKSCKLGRFDYV 770

HSP 2 Score: 150.599 bits (379), Expect = 2.137e-39
Identity = 117/315 (37.14%), Postives = 165/315 (52.38%), Query Frame = 2 
Query:   17 LVKSYAGGEMKEIWICSKCGNENRKENDKCDRKNCYQSKLNARSKEITNHKEIITNQKEELQQNGNEIILNLNQWRCEKCTLINKNNIFQCSACNW--ERKLDCWQCWNGDCNAVNKGVNMFCQKCKLDRNPPDPKTKLVKSYAGGEEKEIWIXXXXXXXXXXXXXXXAGNNCHTFKFYDGTNSTNKDNDRVT-----------VTLHNPNYGKGQKAXXXXXXXXXXXXS---------WKCWFGNCKQLNVAGEEFCKNCRLHRYGTPDPGALVKVRNKNGDVDDMWICHKCFKYNELTDAQCRGKSCKLGRF 895
            + +    G + + WIC KC   N +   KC+ KNC   +     K    + +I T Q E  Q + N+I LN NQW+CEKCTL+N+N  ++C  CN+  +     WQCW  +C   NKG ++FC +CKLDRNPPDP TKLVKSYAG E KEIWIC KC  +N+KE+ KC   NC+  K    +       + +T             + N N  + +K   IN  N+   ++         W+CW G+C  +N     FC+ C+L R   PDP   +      G+  ++WIC KC K N+  DA+C G +C   +F
Sbjct:  338 IERQMGNGRILKFWICEKCWKYNNENFRKCEGKNCGHLRYENAVKWNATNSQINTGQIES-QHSDNKITLNPNQWQCEKCTLVNENRDYKCVVCNFPSQSHRSPWQCWKVNCGKKNKGEDVFCLQCKLDRNPPDPITKLVKSYAGKEIKEIWICEKCGNENRKENDKCDKKNCYQSKLNARSKEITNHKEIITNQKEELQQNGNEIILNLNQWRCEKCTLINKNNIFQCSACNWERKLDCWQCWNGDCNAVNKGVNMFCQKCKLDR-NPPDPKTKLVKSYAGGEEKEIWICSKCKKKNKKEDAKCAGNNCHTFKF 650

HSP 3 Score: 73.9442 bits (180), Expect = 8.178e-14
Identity = 77/262 (29.39%), Postives = 119/262 (45.42%), Query Frame = 2 
Query:  239 WRCEKCTLINKNNIFQCSAC-----NWERKLDCWQCWNGDCNAVNKGVNMFCQKCKLDRNPPDPKTKLVKSYAG-GEEKEIWIXXXXXXXXXXXXXXXAGNNCHTFKF-----YDGTNS--------TNKDNDRVTV----------TLHNPNYGKGQKAXXXXXXXXXXXXSWKCWFGNCKQLNVAGEEFCKNCRLHRYGTPDPGALVKVRNKNGDVDDMWICHKCFKYNELTDAQCRGKSCKLGRFDYV*LLIRSHLQIL 937
            W C  C LIN  N   CSAC     + ER L  W+CW   C  +N     FC+KC L R+ P   + +++   G G   + WIC KC K N +   KC G NC   ++     ++ TNS        +   ++++T+          TL N N  +  K    N  + S+ + W+CW  NC + N   + FC  C+L R   PDP   +       ++ ++WIC KC   N   + +C  K+C   + +     I +H +I+
Sbjct:  271 WMCSWCDLINHINTSICSACEKRDKSVERFL-MWKCWKPHCEHLNNPDCKFCKKCFLQRDAPKSLSDIIERQMGNGRILKFWICEKCWKYNNENFRKCEGKNCGHLRYENAVKWNATNSQINTGQIESQHSDNKITLNPNQWQCEKCTLVNEN--RDYKCVVCNFPSQSHRSPWQCWKVNCGKKNKGEDVFCLQCKLDR-NPPDPITKLVKSYAGKEIKEIWICEKCGNENRKENDKCDKKNCYQSKLNARSKEITNHKEII 528
[Close]
BLAST of SMED30028732 vs. Planmine SMEST
Match: SMESG000022815.1 (SMESG000022815.1)

HSP 1 Score: 491.115 bits (1263), Expect = 1.059e-168
Identity = 271/301 (90.03%), Postives = 281/301 (93.36%), Query Frame = 2 
Query:    2 DPITKLVKSYAGGEMKEIWICSKCGNENRKENDKCDRKNCYQSKLNARSKEITNHKEIITNQKEELQQNGNEIILNLNQWRCEKCTLINKNNIFQCSACNWERKLDCWQCWNGDCNAVNKGVNMFCQKCKLDRNPPDPKTKLVKSYAGGEEKEIWIXXXXXXXXXXXXXXXAGNNCHTFKFYDGTNSTNKDNDRVTVTLHNPNYGKGQKAXXXXXXXXXXXXSWKCWFGNCKQLNVAGEEFCKNCRLHRYGTPDPGALVKVRNKNGDVDDMWICHKCFKYNELTDAQCRGKSCKLGRFDYV 904
            DPITKLVKSYA  E+KEIWICSKCGNENRKENDKCDRKNCYQSKLNARSKEI N +EIITNQKEELQQNGNEIILN NQWRCEKCT+INKNN+FQCSACNWERKLDCWQCWNGDC AVNKGVNMFCQKCKLDRNPPDPKTKLVKSY G E KEIWICSKCKKKNKKEDAKCAGN C+ FKF+DGTNSTNKDND+V VT HNPNY KGQKANNINNEN SNGNSWKCWF NCKQLNVAGEEFCKNCRLHRYGT DPGALVKVR+KNGDVDDMWICHKCF YNEL + QCRGKSC+LGRFDYV
Sbjct:  430 DPITKLVKSYAEKEIKEIWICSKCGNENRKENDKCDRKNCYQSKLNARSKEIINDEEIITNQKEELQQNGNEIILNSNQWRCEKCTVINKNNVFQCSACNWERKLDCWQCWNGDCKAVNKGVNMFCQKCKLDRNPPDPKTKLVKSYTGSEMKEIWICSKCKKKNKKEDAKCAGNKCYVFKFFDGTNSTNKDNDKVAVTQHNPNYCKGQKANNINNENPSNGNSWKCWFANCKQLNVAGEEFCKNCRLHRYGTWDPGALVKVRSKNGDVDDMWICHKCFMYNELMEGQCRGKSCRLGRFDYV 730

HSP 2 Score: 269.626 bits (688), Expect = 5.773e-83
Identity = 174/318 (54.72%), Postives = 210/318 (66.04%), Query Frame = 2 
Query:    2 DPITKLVKSYAGGEMKEIWICSKCGNENRKENDKCDRKNCYQSKLNARSKEITNHKEIITNQKEELQQNGNEIILNLNQWRCEKCTLINKNNIFQCSACNWERKLDCWQCWNGDCNAVNKGVNMFCQKCKLDRNPPDPKTKLVKSYAGGEEKEIWIXXXXXXXXXXXXXXXAGNNCHTFKFYDGTNSTNKDNDRVT-----------VTLHNPNYGKGQKAXXXXXXXXXXXXS---------WKCWFGNCKQLNVAGEEFCKNCRLHRYGTPDPGALVKVRNKNGDVDDMWICHKCFKYNELTDAQCRGKSCKLGRF 895
            DPITKLVKSYAGGEMKEIWICSKCGNENRKENDKCDRKNCYQSKLNARSKEITNHKEIITNQKEELQQNGNEIILN N+WRC KC LIN    + C+AC++  +   WQCWN DC  +NKGVN FCQ+C+LDRNPPDP TKLVKSYA  E KEIWICSKC  +N+KE+ KC   NC+  K    +     D + +T             + N N  + +K   IN  N+   ++         W+CW G+CK +N     FC+ C+L R   PDP   +       ++ ++WIC KC K N+  DA+C G  C + +F
Sbjct:  298 DPITKLVKSYAGGEMKEIWICSKCGNENRKENDKCDRKNCYQSKLNARSKEITNHKEIITNQKEELQQNGNEIILNSNKWRCGKCNLIND---YNCAACDFPSQSS-WQCWNIDCEKMNKGVNKFCQQCRLDRNPPDPITKLVKSYAEKEIKEIWICSKCGNENRKENDKCDRKNCYQSKLNARSKEIINDEEIITNQKEELQQNGNEIILNSNQWRCEKCTVINKNNVFQCSACNWERKLDCWQCWNGDCKAVNKGVNMFCQKCKLDR-NPPDPKTKLVKSYTGSEMKEIWICSKCKKKNKKEDAKCAGNKCYVFKF 610

HSP 3 Score: 154.451 bits (389), Expect = 9.976e-41
Identity = 112/312 (35.90%), Postives = 166/312 (53.21%), Query Frame = 2 
Query:   17 LVKSYAGGEMKEIWICSKCGNENRKENDKCDRKNCYQSKLNARSKEITNHKEIITNQKEELQQNGNEIILNLNQWRCEKCTLINKNNIFQCSACNW--ERKLDCWQCWNGDCNAVNKGVNMFCQKCKLDRNPPDPKTKLVKSYAGGEEKEIWIXXXXXXXXXXXXXXXAGNNCHTFKFYDGTNSTNKDNDRVT------------VTLHNPNYGKGQ----KAXXXXXXXXXXXXSWKCWFGNCKQLNVAGEEFCKNCRLHRYGTPDPGALVKVRNKNGDVDDMWICHKCFKYNELTDAQCRGKSCKLGRFD 898
            + +    G + + WIC KC   N +   KC+ KNC   +     K    + +I T Q E  Q + N+I LN NQW+CEKCTL+N+N  ++C  CN+  +     WQCW  +C   NKG ++FC +CKLDRNPPDP TKLVKSYAGGE KEIWICSKC  +N+KE+ KC   NC+  K    +       + +T            + L++  +  G+       N    +  + +SW+CW  +C+++N    +FC+ CRL R   PDP   +       ++ ++WIC KC   N   + +C  K+C   + +
Sbjct:  166 IERQMGNGSILKFWICEKCWKYNNENFRKCEGKNCGHPRYGNAVKWNATNSQINTGQIES-QHSDNKITLNPNQWQCEKCTLVNENRDYKCVLCNFPSQSHQSPWQCWKVNCGKKNKGEDVFCLQCKLDRNPPDPITKLVKSYAGGEMKEIWICSKCGNENRKENDKCDRKNCYQSKLNARSKEITNHKEIITNQKEELQQNGNEIILNSNKWRCGKCNLINDYNCAACDFPSQSSWQCWNIDCEKMNKGVNKFCQQCRLDR-NPPDPITKLVKSYAEKEIKEIWICSKCGNENRKENDKCDRKNCYQSKLN 475

HSP 4 Score: 75.8702 bits (185), Expect = 1.708e-14
Identity = 79/262 (30.15%), Postives = 120/262 (45.80%), Query Frame = 2 
Query:  239 WRCEKCTLINKNNIFQCSAC-----NWERKLDCWQCWNGDCNAVNKGVNMFCQKCKLDRNPPDPKTKLVKSYAG-GEEKEIWIXXXXXXXXXXXXXXXAGNNCHTFKF-----YDGTNS--------TNKDNDRVTV----------TLHNPNYGKGQKAXXXXXXXXXXXXSWKCWFGNCKQLNVAGEEFCKNCRLHRYGTPDPGALVKVRNKNGDVDDMWICHKCFKYNELTDAQCRGKSCKLGRFDYV*LLIRSHLQIL 937
            W C  C LIN  N   CSAC     N ER L  W+CW   C  +N     FC+KC L R+ P   + +++   G G   + WIC KC K N +   KC G NC   ++     ++ TNS        +   ++++T+          TL N N  +  K    N  + S+ + W+CW  NC + N   + FC  C+L R   PDP   +      G++ ++WIC KC   N   + +C  K+C   + +     I +H +I+
Sbjct:   99 WMCSWCDLINHINTSICSACEKSDKNVERFL-MWKCWKPHCEQLNNPDCKFCKKCFLQRDAPKSLSDIIERQMGNGSILKFWICEKCWKYNNENFRKCEGKNCGHPRYGNAVKWNATNSQINTGQIESQHSDNKITLNPNQWQCEKCTLVNEN--RDYKCVLCNFPSQSHQSPWQCWKVNCGKKNKGEDVFCLQCKLDR-NPPDPITKLVKSYAGGEMKEIWICSKCGNENRKENDKCDRKNCYQSKLNARSKEITNHKEII 356
[Close]
BLAST of SMED30028732 vs. Planmine SMEST
Match: SMESG000022780.1 (SMESG000022780.1)

HSP 1 Score: 62.3882 bits (150), Expect = 3.497e-10
Identity = 69/310 (22.26%), Postives = 125/310 (40.32%), Query Frame = 2 
Query:   29 YAGGEMKEIWICSKCGNENRKENDKCDR-KNCYQSKLNARSKEITNHKEIITNQKEELQQNG----NEIILNLNQWRCEKCTLINKNNIFQCSAC-NWERKL-------------DCWQCWNGDCNAVNKGVNMFCQKCKLDRNPPDPKTKLVKSYAGGEEKEIWIXXXXXXXXXXXXXXXAGNNCHTFKFYDGTNSTNK-DNDRVTVTLHNPNYGKGQKAXXXXXXXXXXXXSWKCWFGNCKQLNVAGEEFCKNCRLHRYGTPDPGALVKVRNKNGDVDDMWICHKCFKYNELTDAQCRGKSCKLGRFD 898
            +AG      + C KC  EN+ +  +C        S L A ++ +   KE I   +   Q N     N+   N +QW+CE C   N+    +C+ C N  R+              + W CW   C   N G + +C  C +D+     + +++     G ++++WI                   C    F++G   ++K +    +      N  +  +    N        +W CW  NC++ N    ++C +C + +    + G ++ V  K+G  + MWIC+ C  +N   +A+   KSC   R++
Sbjct:  121 FAGKLGITSFKCEKCATENQIDELECSMCGESVMSYLKANNRALGELKEKINKNENNEQMNNISMKNKNDENSSQWKCEYCGNANEFLSNRCAGCPNLRRESNDRSDNPAGGEIDENWDCWWRGCMKRNYGNHQYCSHCGVDKAANQNRGEIINVLKDGRKEKMWI-------------------CTKCGFHNGDLDSDKCEICECSRKEEKENKSEQNRGRLGNPAGGEIDENWDCWSRNCRERNYGNHQYCSHCGVDKAANQNRGEIINVF-KDGRKEKMWICNGCNHFNRDINAKI-CKSCNESRYE 409

HSP 2 Score: 55.4546 bits (132), Expect = 5.361e-8
Identity = 65/297 (21.89%), Postives = 117/297 (39.39%), Query Frame = 2 
Query:   14 KLVKSYAGGEMKEIWICSKCGNENRK-ENDKCDRKNCYQSKLNARSKEITNHKEIITNQKEELQQNGNEIILNLNQWRCEKCTLINKNNIFQCSACNWERKLDCWQCWNGDCNAVNKGVNMFCQKCKLDRNPPDPKTKLVKSYAGGEEKEIWIXXXXXXXXXXXXXXXAGNNCHTFKFYDGTNSTNKD-NDRVTVTLHNPNYGKGQKAXXXXXXXXXXXXSWKCWFGNCKQLNVAGEEFCKNCRLHRYGTPDPGALVKVRNKNGDVDDMWICHKCFKYNELTDAQCRGKSCKLGRFD 898
            +++     G  +++WIC+KCG  N   ++DKC+   C      +R +E  N  E   N+       G EI  N                               W CW+ +C   N G + +C  C +D+     + +++  +  G ++++WIC                         +G N  N+D N ++  + +   Y        I +       +WKC    C + NV  +++C  C + R    + G ++ V   NG  + MWIC++C  +N   +A+   KSC   R++
Sbjct:  271 EIINVLKDGRKEKMWICTKCGFHNGDLDSDKCEICEC------SRKEEKENKSE--QNRGRLGNPAGGEIDEN-------------------------------WDCWSRNCRERNYGNHQYCSHCGVDKAANQNRGEIINVFKDGRKEKMWIC-------------------------NGCNHFNRDINAKICKSCNESRY-------EIESAINKANENWKCQQSICGKANVGSDKYCSRCGVDRAANQNRGEVIGVL-LNGRKEKMWICNECNHFNRDINAKI-CKSCNESRYE 494
[Close]
BLAST of SMED30028732 vs. Planmine SMEST
Match: SMESG000022814.1 (SMESG000022814.1)

HSP 1 Score: 53.5286 bits (127), Expect = 2.333e-7
Identity = 41/197 (20.81%), Postives = 84/197 (42.64%), Query Frame = 2 
Query:  323 WQCWNGDCNAVNKGVNMFCQKCKLDRNPPDPKTKLVKSYAGGEEKEIWIXXXXXXXXXXXXXXXAGNNCHTFKFYDGTNSTNKDNDRVTV-----TLHNPNYGKGQKAXXXXXXXXXXXXSWKCWFGNCKQLNVAGEEFCKNCRLHRYGTPDPGALVKVRNKNGDVDDMWICHKCFKYNELTDAQCRGKSCKLGRFD 898
            W CW   C   N G + +C  C +D+     + +++     G ++++WI                   C    F++G    + D+D+  +          N  +  +    N+       +W CW  +C++ N    ++C +C + +    + G ++ V  K+G  ++MWIC++C  +N   +A+   KSC   R++
Sbjct:  218 WDCWRRGCMKKNYGNHQYCSHCGVDKAANQNRGEIINVLKDGRKEKMWI-------------------CTKCGFHNG----DLDSDKCGICECSRKEERENKSEQNRGRLGNSAGGEIDENWDCWSRSCRKRNYGNHQYCSHCGVDKAANQNRGEIINVL-KDGRKEEMWICNECNHFNWDINAKI-CKSCNESRYE 389

HSP 2 Score: 53.1434 bits (126), Expect = 2.753e-7
Identity = 58/235 (24.68%), Postives = 102/235 (43.40%), Query Frame = 2 
Query:  203 QNGNEIILNLNQWRCEKCTLINKNNIFQ-------CSACNWER-KLDC--------WQCWNGDCNAVNKGVNMFCQKCKLDRNPPDPKTKLVKSYAGGEEKEIWIXXXXXXXXXXXXXXXAGNNCHTFKFYDGTNSTNKDNDRVTVTLHNPNYGKGQKAXXXXXXXXXXXXSWKCWFGNCKQLNVAGEEFCKNCRLHRYGTPDPGALVKVRNKNGDVDDMWICHKCFKYNELTDA 859
            QN  EII  L   R E+  + N+ N F        C +CN  R +++C        W+C    C   N G + +C +C +DR     + +++     G ++++WIC+KC   N   D+      C   +  +  N   ++  R    L NP  G+  +             +W CW  +C++ N    ++C +C + R    + G ++    K+G  + +WIC KC  +N   D+
Sbjct:  345 QNRGEIINVLKDGRKEEMWICNECNHFNWDINAKICKSCNESRYEIECAINKANENWKCQQSICGKANVGSDKYCSRCGVDRAANQNRGEIINVLKDGRKEKMWICTKCGLFNGDLDSDKC-EICECSRKEEKENKYEQNRGR----LGNPAGGEIDE-------------NWDCWSRSCRKRNYGNHQYCSHCGVDRAANQNRGEIINAF-KDGKEEKIWICTKCGLFNGDLDS 560

HSP 3 Score: 52.7582 bits (125), Expect = 3.867e-7
Identity = 62/286 (21.68%), Postives = 105/286 (36.71%), Query Frame = 2 
Query:   14 KLVKSYAGGEMKEIWICSKCGNENRK-ENDKCDRKNCYQSKLNARSKEITNHKEIITNQKEELQQNGNEIILNLNQWRCEKCTLINKNNIFQCSACNWERKLDCWQCWNGDCNAVNKGVNMFCQKCKLDRNPPDPKTKLVKSYAGGEEKEIWIXXXXXXXXXXXXXXXAGNNCHTFKFYDGTN---STNKDNDRVTVTLHNPNYGKGQKAXXXXXXXXXXXXSWKCWFGNCKQLNVAGEEFCKNCRLHRYGTPDPGALVKVRNKNGDVDDMWICHKCFKYNELTDA 859
            +++     G  +++WIC+KCG  N   ++DKC    C      +R +E  N  E   N+       G EI  N                               W CW+  C   N G + +C  C +D+     + +++     G ++E+WIC                N C+ F +        S N+    +   ++  N                   +WKC    C + NV  +++C  C + R    + G ++ V  K+G  + MWIC KC  +N   D+
Sbjct:  251 EIINVLKDGRKEKMWICTKCGFHNGDLDSDKCGICEC------SRKEERENKSE--QNRGRLGNSAGGEIDEN-------------------------------WDCWSRSCRKRNYGNHQYCSHCGVDKAANQNRGEIINVLKDGRKEEMWIC----------------NECNHFNWDINAKICKSCNESRYEIECAINKAN------------------ENWKCQQSICGKANVGSDKYCSRCGVDRAANQNRGEIINVL-KDGRKEKMWICTKCGLFNGDLDS 462
The following BLAST results are available for this feature:
BLAST of SMED30028732 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028732 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028732 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028732 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028732 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028732 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028732 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028732 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028732 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028732 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028732 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028732 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028732 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028732 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 4
Match NameE-valueIdentityDescription
SMESG000022822.12.137e-3998.67SMESG000022822.1[more]
SMESG000022815.11.059e-16890.03SMESG000022815.1[more]
SMESG000022780.13.497e-1022.26SMESG000022780.1[more]
SMESG000022814.12.333e-720.81SMESG000022814.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30028732 ID=SMED30028732|Name=SMED30028732|organism=Schmidtea mediterranea sexual|type=transcript|length=1265bp
AGATCCTATTACAAAACTTGTTAAAAGCTACGCAGGAGGTGAAATGAAAG
AAATTTGGATTTGTAGTAAATGTGGAAATGAGAATCGAAAAGAGAATGAT
AAATGTGATAGAAAGAATTGTTACCAATCCAAGTTGAATGCGAGGTCGAA
AGAGATTACAAATCATAAAGAAATAATTACCAATCAGAAGGAAGAATTAC
AACAGAATGGCAATGAAATAATATTAAATTTAAATCAATGGCGATGTGAA
AAATGCACTTTAATCAATAAGAACAATATTTTTCAATGCTCAGCATGTAA
TTGGGAACGGAAACTCGATTGTTGGCAGTGTTGGAATGGGGATTGCAATG
CAGTGAATAAAGGCGTGAATATGTTTTGTCAGAAATGCAAATTAGACCGA
AATCCGCCTGATCCTAAAACGAAACTTGTTAAAAGTTACGCAGGAGGTGA
AGAGAAAGAGATCTGGATTTGCAGTAAATGTAAAAAGAAGAATAAAAAGG
AGGATGCTAAATGTGCTGGAAATAATTGTCACACATTTAAATTCTATGAC
GGAACAAATTCCACCAATAAAGATAATGATAGGGTGACAGTTACTCTACA
TAATCCCAATTACGGTAAAGGTCAAAAGGCAAATAATATTAATAATGAGA
ATCTATCAAATGGAAATTCATGGAAATGTTGGTTTGGGAACTGTAAACAA
TTAAACGTTGCTGGAGAGGAATTCTGTAAGAATTGCAGATTACATAGATA
CGGAACACCGGATCCGGGCGCCCTGGTGAAAGTGAGAAATAAGAACGGTG
ATGTTGATGATATGTGGATATGCCACAAGTGCTTTAAGTATAATGAACTG
ACGGATGCGCAATGCCGCGGTAAATCCTGCAAATTAGGGCGTTTTGACTA
TGTTTAATTATTAATCCGATCACATTTACAAATATTATTCTGGTAATTTT
ATGAAAATGATTTCATTTCTTTATAAATTTAATTGCTATTGAAATACATT
TTGTGAGTCTCCAGGCCCTGAAAATCCTGGATCATTGATCTGGCAGCTGA
ATCACTGAGTTTATCAGACTTTATTCTTGCCGTGCATTGTTATGCGTTTT
ATTTGATGAAGAAACTTTATATTGATCTGATTCGTTATTTCGTGATATAG
GCGGTCTATATGTTACCCGTTCTGGATGCTTTAAATATTGCTTGCATTTC
CTGGAAATCGCTAGTTAACCGCAAAATTGAACTGCTCTTGGCAAATTATA
ATTTATCTTCAAAAT
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protein sequence of SMED30028732-orf-1

>SMED30028732-orf-1 ID=SMED30028732-orf-1|Name=SMED30028732-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=302bp
DPITKLVKSYAGGEMKEIWICSKCGNENRKENDKCDRKNCYQSKLNARSK
EITNHKEIITNQKEELQQNGNEIILNLNQWRCEKCTLINKNNIFQCSACN
WERKLDCWQCWNGDCNAVNKGVNMFCQKCKLDRNPPDPKTKLVKSYAGGE
EKEIWICSKCKKKNKKEDAKCAGNNCHTFKFYDGTNSTNKDNDRVTVTLH
NPNYGKGQKANNINNENLSNGNSWKCWFGNCKQLNVAGEEFCKNCRLHRY
GTPDPGALVKVRNKNGDVDDMWICHKCFKYNELTDAQCRGKSCKLGRFDY
V*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000034epidermis
PLANA:0000099neuron
Vocabulary: INTERPRO
TermDefinition
IPR001876Znf_RanBP2
Vocabulary: molecular function
TermDefinition
GO:0008270zinc ion binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 49..69
IPR001876Zinc finger, RanBP2-typeSMARTSM00547zf_4coord: 17..43
e-value: 0.73
score: 13.0
coord: 270..296
e-value: 8.5
score: 4.5
coord: 78..102
e-value: 0.37
score: 15.3
coord: 153..179
e-value: 1.7
score: 10.1
IPR001876Zinc finger, RanBP2-typePROSITEPS01358ZF_RANBP2_1coord: 224..245
IPR001876Zinc finger, RanBP2-typePROSITEPS01358ZF_RANBP2_1coord: 80..99
IPR001876Zinc finger, RanBP2-typePROSITEPS01358ZF_RANBP2_1coord: 108..129
IPR001876Zinc finger, RanBP2-typePROSITEPS50199ZF_RANBP2_2coord: 76..105
score: 8.377