Elongator complex protein 2 (inferred by orthology to a human protein)
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30028703 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 1
Homology
BLAST of Elongator complex protein 2 (inferred by orthology to a human protein) vs. Ensembl Fly
Match: Elp2 (gene:FBgn0033540 transcript:FBtr0088212) HSP 1 Score: 50.8322 bits (120), Expect = 9.753e-6 Identity = 50/157 (31.85%), Postives = 71/157 (45.22%), Query Frame = 3 Query: 1437 QD*SFGYSV--RLHRNNPVFNVYIADIDDFH--RCFVTVSRDKRMIIWDSD--------NCARVGEPLTLK-ESINAIATMPILL---ENLYVFAIGLENGEIKIVQFDLSTNSWTTLLSF---VAHFDWITRLRFNPVPNENKFLLASCSKDRFVK 1850 QD S Y + ++N V I D H + FVT SRD ++++W + N + L LK ESI A+A L ++ Y+ A+G E G IKI QF +W L AH + RL+F P + LASC +D V+ Sbjct: 636 QDSSVSYQLVASTDKSNGVHTRIIWSCDWSHDGQFFVTSSRDGKVVVWKKEEDCKESSLNGWQANGVLELKNESITAVAFSNSYLSGTDDTYILALGTETGLIKIYQF--VRGAWKLLSDLNKSQAHHLTVRRLQFRP---GKQLQLASCGEDHLVR 787
BLAST of Elongator complex protein 2 (inferred by orthology to a human protein) vs. TrEMBL
Match: R7TWN9 (Uncharacterized protein OS=Capitella teleta OX=283909 GN=CAPTEDRAFT_179061 PE=4 SV=1) HSP 1 Score: 71.633 bits (174), Expect = 1.138e-8 Identity = 40/115 (34.78%), Postives = 60/115 (52.17%), Query Frame = 3 Query: 1530 FVTVSRDKRMIIWDSDNC-----ARVGEPLTLKESINAIATMPILLENLYVFAIGLENGEIKIVQFDLSTNSWT---TLLSFVAHFDWITRLRFNPVPNENKFLLASCSKDRFVK 1850 FVTVSRDK+ ++W G PL L +S+ A+ P + + Y+ A GL+NG I I ++ + +W+ L AH + +++F P N+ LLASCS D VK Sbjct: 673 FVTVSRDKKAVVWQQQPLNDELFVSAGSPLKLPDSVTAVDFAPDMHNDFYILAFGLDNGHIMIYKW--ADQAWSLHFHLKQSEAHHLTVKQVKFQPTAAGNELLLASCSDDHSVK 785
BLAST of Elongator complex protein 2 (inferred by orthology to a human protein) vs. TrEMBL
Match: A0A3P6NT87 (WD_REPEATS_REGION domain-containing protein OS=Anisakis simplex OX=6269 GN=ASIM_LOCUS1744 PE=4 SV=1) HSP 1 Score: 65.4698 bits (158), Expect = 3.147e-8 Identity = 51/136 (37.50%), Postives = 69/136 (50.74%), Query Frame = 3 Query: 1524 RCFVTVSRDKRMIIW------DSDNCARVGEPLTLK--ESINAIATMPILLENLYVFAIGLENGEIKIVQFDLSTNSWT--TLLSFV----AHFDWITRLRFNPVP-------------NENKFLLASCSKDRFVK 1850 R F T +RDK++ +W + + V L K ES+ AIA P LL YV AIGLENG+I++V ++ S + T +LL+ + AH I RLRF P P NEN + LAS + D VK Sbjct: 25 RYFATGARDKKVALWSVAANDNEEKSTHVQIALQSKRSESVTAIAFCPCLLNQSYVLAIGLENGQIELVAWNPSIGTATDLSLLASLDGCRAHSQTIARLRFRPKPGKWMSVCESEKSTNENIYELASVANDNAVK 160
BLAST of Elongator complex protein 2 (inferred by orthology to a human protein) vs. TrEMBL
Match: A0A0M3J2W2 (Elongator complex protein 2 (inferred by orthology to a human protein) OS=Anisakis simplex OX=6269 PE=4 SV=1) HSP 1 Score: 65.0846 bits (157), Expect = 3.239e-8 Identity = 51/136 (37.50%), Postives = 69/136 (50.74%), Query Frame = 3 Query: 1524 RCFVTVSRDKRMIIW------DSDNCARVGEPLTLK--ESINAIATMPILLENLYVFAIGLENGEIKIVQFDLSTNSWT--TLLSFV----AHFDWITRLRFNPVP-------------NENKFLLASCSKDRFVK 1850 R F T +RDK++ +W + + V L K ES+ AIA P LL YV AIGLENG+I++V ++ S + T +LL+ + AH I RLRF P P NEN + LAS + D VK Sbjct: 25 RYFATGARDKKVALWSVAANDNEEKSTHVQIALQSKRSESVTAIAFCPCLLNQSYVLAIGLENGQIELVAWNPSIGTATDLSLLASLDGCRAHSQTIARLRFRPKPGKWMSVCESEKSTNENIYELASVANDNAVK 160
BLAST of Elongator complex protein 2 (inferred by orthology to a human protein) vs. TrEMBL
Match: H2Y854 (WD_REPEATS_REGION domain-containing protein OS=Ciona savignyi OX=51511 PE=4 SV=1) HSP 1 Score: 65.4698 bits (158), Expect = 8.172e-7 Identity = 41/116 (35.34%), Postives = 67/116 (57.76%), Query Frame = 3 Query: 1530 FVTVSRDKRMIIWDSDN------CARVGEPLTLKESINAIATMPILLENLYVFAIGLENGEIKIVQFDLSTNSWTTLLSF---VAHFDWITRLRFNPVPNENKFLLASCSKDRFVK 1850 F+T SRDK+++IW ++ CA + KE++ A+A P +L N Y+ A GLE+G IK+ +F S + + + F ++H + RL++NP+ +K LASC+ D VK Sbjct: 639 FITASRDKKIMIWKNETNSVDKVCAEI-----CKEAVTAVAFSPQILSNKYIIAYGLESGIIKLAEF--SNSEFQNIFQFNINMSHCLAVKRLKWNPL--NSKLTLASCALDNQVK 745
BLAST of Elongator complex protein 2 (inferred by orthology to a human protein) vs. TrEMBL
Match: A0A4Y2H3G9 (Elongator complex protein 2 OS=Araneus ventricosus OX=182803 GN=elp2_0 PE=4 SV=1) HSP 1 Score: 64.3142 bits (155), Expect = 2.128e-6 Identity = 45/129 (34.88%), Postives = 66/129 (51.16%), Query Frame = 3 Query: 1512 DDFHRCFVTVSRDKRMIIW-----DSDNCARVGEPLTLKESINAIATMPILLENLYVFAIGLENGEIKIVQFDLSTNSWTTLLSF---VAHFDWITRLRFNPV--------PNENKFLLASCSKDRFVK 1850 D H F T SRDK++ +W +S + +PL +++S A++ P L++N Y+ AIGLENG I + F T W L +AH + RL+F+PV N + LASCS D +K Sbjct: 662 DSLH--FATSSRDKKVAMWGKNKDESPEFSLCSKPLDVEDSATAVSIAPCLVKNKYLTAIGLENGSIILSIFSNDTG-WEILKKLDQNIAHHLNVNRLKFSPVLGEAGCREKNTDVLQLASCSNDHHMK 787
BLAST of Elongator complex protein 2 (inferred by orthology to a human protein) vs. Planmine SMEST
Match: SMESG000022611.1 (SMESG000022611.1) HSP 1 Score: 226.868 bits (577), Expect = 5.255e-62 Identity = 109/109 (100.00%), Postives = 109/109 (100.00%), Query Frame = 3 Query: 1524 RCFVTVSRDKRMIIWDSDNCARVGEPLTLKESINAIATMPILLENLYVFAIGLENGEIKIVQFDLSTNSWTTLLSFVAHFDWITRLRFNPVPNENKFLLASCSKDRFVK 1850 RCFVTVSRDKRMIIWDSDNCARVGEPLTLKESINAIATMPILLENLYVFAIGLENGEIKIVQFDLSTNSWTTLLSFVAHFDWITRLRFNPVPNENKFLLASCSKDRFVK Sbjct: 601 RCFVTVSRDKRMIIWDSDNCARVGEPLTLKESINAIATMPILLENLYVFAIGLENGEIKIVQFDLSTNSWTTLLSFVAHFDWITRLRFNPVPNENKFLLASCSKDRFVK 709 The following BLAST results are available for this feature:
BLAST of Elongator complex protein 2 (inferred by orthology to a human protein) vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of Elongator complex protein 2 (inferred by orthology to a human protein) vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of Elongator complex protein 2 (inferred by orthology to a human protein) vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 1
BLAST of Elongator complex protein 2 (inferred by orthology to a human protein) vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of Elongator complex protein 2 (inferred by orthology to a human protein) vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of Elongator complex protein 2 (inferred by orthology to a human protein) vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of Elongator complex protein 2 (inferred by orthology to a human protein) vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of Elongator complex protein 2 (inferred by orthology to a human protein) vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Elongator complex protein 2 (inferred by orthology to a human protein) vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of Elongator complex protein 2 (inferred by orthology to a human protein) vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of Elongator complex protein 2 (inferred by orthology to a human protein) vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of Elongator complex protein 2 (inferred by orthology to a human protein) vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of Elongator complex protein 2 (inferred by orthology to a human protein) vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of Elongator complex protein 2 (inferred by orthology to a human protein) vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 1
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30028703 ID=SMED30028703|Name=Elongator complex protein 2 (inferred by orthology to a human protein)|organism=Schmidtea mediterranea sexual|type=transcript|length=3972bpback to top protein sequence of SMED30028703-orf-1 >SMED30028703-orf-1 ID=SMED30028703-orf-1|Name=SMED30028703-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=115bp MHAIININFKGCITGTRKSIITIQLHNSVYFEMTVSFSFSKESPSVTNSYback to top protein sequence of SMED30028703-orf-2 >SMED30028703-orf-2 ID=SMED30028703-orf-2|Name=SMED30028703-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=107bp MIIWDSDNCARVGEPLTLKESINAIATMPILLENLYVFAIGLENGEIKIVback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
|