two pore calcium channel protein 1

Overview
Nametwo pore calcium channel protein 1
Smed IDSMED30028664
Length (bp)2350
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of two pore calcium channel protein 1 (SMED30028664) t-SNE clustered cells

Violin plots show distribution of expression levels for two pore calcium channel protein 1 (SMED30028664) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of two pore calcium channel protein 1 (SMED30028664) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for two pore calcium channel protein 1 (SMED30028664) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30028664

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 6

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
protonephridiaSMED30028664h1SMcG0002160 dd_Smed_v4_8333_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30028664h1SMcG0002160 dd_Smed_v4_8333_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30028664h1SMcG0002160 dd_Smed_v4_8333_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30028664h1SMcG0002160 dd_Smed_v4_8333_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30028664h1SMcG0002160 dd_Smed_v4_8333_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
glial cellSMED30028664h1SMcG0002160 dd_Smed_v6_8333_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of two pore calcium channel protein 1 vs. Ensembl Human
Match: TPCN1 (two pore segment channel 1 [Source:HGNC Symbol;Acc:HGNC:18182])

HSP 1 Score: 90.5077 bits (223), Expect = 5.838e-18
Identity = 125/558 (22.40%), Postives = 258/558 (46.24%), Query Frame = 1 
Query:  706 PYMQNFFDIVWETLIFITSSNSPDVILPGYSKSSYIGILFLIFYAM-FYFLLIFVYGLIMGNFRYHTKEEMTKLLVGKISLMYDAFVLLAEKIVINEKKNCNELFEKNIIKKETWKNLIARFKKYQNCEEAEEIMYSLLSQTNKTYLMKNEFLQMADLMSIPLTIVTNKFHFFE---KNFWFVFSNKLADFVESKYFNLIVGIVIVINSCCI---TFTSE---------PYXXXXXXXXXXXXXXXRIYVKGFIRYFHSLWNSFDFVVIVLAFIFWFVENMKLNVQTITFTXXXXXXXXXXXXXXGIAAYHQLLHILGMLIPTISSL-ITIV-------------FCFIVFYVCIGIWSYQDMFLMNRSELDYMFNSSSCNSTKLEGSDFGRQHYCILSFNSFISGFLTLCSVSIGNNWHTITEGYELESESMILSRFYFSVFKGVLTYLLLLIVAFVLDVFLLAYN---QKSSLKIEELIKNKLNSLKINFISYGEI-RKSRLTKTDSTEGSQSISLTRYFKRVKFEETSRIWFRLKAKGARSVEYVSQAMFQEEIE 2277
            PY     + +    + +T++N PDV++P YS++ +  + F+++ ++  YF++  +  ++   F    K +   LL+ K + +  A+ LL     I++++          I    ++ L+ RF K +       + +  L+Q N   L   +F  + ++ ++      N+ H+F+   +    +F   +   V+SK F   + +V+ +N   I   TF  +         P++ L F  ++ +E+ L++   G + Y  S WN FDF V V AF+   +  + LN++   F +++     L         Y  +L  +  L+P ++SL +T++             FC IVF  C    +  D +           N +  N T +E       +Y + +F++ ++ F+TL  +++ NNW+ I EG  + S++   SR YF  F  V   ++ +IVAF+L+ F+   N   +    +++  I  +    K   ++  E+ R++R   +D T   +++S     +  ++++ S ++     + +R+   +S  M+QEEI+
Sbjct:  193 PYFSTLENSIVSLFVLLTTANFPDVMMPSYSRNPWSCVFFIVYLSIELYFIMNLLLAVVFDTFNDIEKRKFKSLLLHKRTAIQHAYRLL-----ISQRRPAG-------ISYRQFEGLM-RFYKPRMSARERYLTFKALNQNNTPLLSLKDFYDIYEVAALKWKAKKNREHWFDELPRTALLIFKG-INILVKSKAFQYFMYLVVAVNGVWILVETFMLKGGNFFSKHVPWSYLVFLTIYGVELFLKVAGLGPVEYLSSGWNLFDFSVTVFAFL--GLLALALNMEPFYFIVVLRPLQLLRLFKLK-ERYRNVLDTMFELLPRMASLGLTLLIFYYSFAIVGMEFFCGIVFPNCCNTSTVADAYRWR--------NHTVGNRTVVEEG-----YYYLNNFDNILNSFVTLFELTVVNNWYIIMEG--VTSQTSHWSRLYFMTFYIVTMVVMTIIVAFILEAFVFRMNYSRKNQDSEVDGGITLEKEISKEELVAVLELYREARGASSDVTRLLETLS-----QMERYQQHSMVFL---GRRSRTKSDLSLKMYQEEIQ 710
[Close]
BLAST of two pore calcium channel protein 1 vs. Ensembl Human
Match: TPCN1 (two pore segment channel 1 [Source:HGNC Symbol;Acc:HGNC:18182])

HSP 1 Score: 88.9669 bits (219), Expect = 1.509e-17
Identity = 125/558 (22.40%), Postives = 258/558 (46.24%), Query Frame = 1 
Query:  706 PYMQNFFDIVWETLIFITSSNSPDVILPGYSKSSYIGILFLIFYAM-FYFLLIFVYGLIMGNFRYHTKEEMTKLLVGKISLMYDAFVLLAEKIVINEKKNCNELFEKNIIKKETWKNLIARFKKYQNCEEAEEIMYSLLSQTNKTYLMKNEFLQMADLMSIPLTIVTNKFHFFE---KNFWFVFSNKLADFVESKYFNLIVGIVIVINSCCI---TFTSE---------PYXXXXXXXXXXXXXXXRIYVKGFIRYFHSLWNSFDFVVIVLAFIFWFVENMKLNVQTITFTXXXXXXXXXXXXXXGIAAYHQLLHILGMLIPTISSL-ITIV-------------FCFIVFYVCIGIWSYQDMFLMNRSELDYMFNSSSCNSTKLEGSDFGRQHYCILSFNSFISGFLTLCSVSIGNNWHTITEGYELESESMILSRFYFSVFKGVLTYLLLLIVAFVLDVFLLAYN---QKSSLKIEELIKNKLNSLKINFISYGEI-RKSRLTKTDSTEGSQSISLTRYFKRVKFEETSRIWFRLKAKGARSVEYVSQAMFQEEIE 2277
            PY     + +    + +T++N PDV++P YS++ +  + F+++ ++  YF++  +  ++   F    K +   LL+ K + +  A+ LL     I++++          I    ++ L+ RF K +       + +  L+Q N   L   +F  + ++ ++      N+ H+F+   +    +F   +   V+SK F   + +V+ +N   I   TF  +         P++ L F  ++ +E+ L++   G + Y  S WN FDF V V AF+   +  + LN++   F +++     L         Y  +L  +  L+P ++SL +T++             FC IVF  C    +  D +           N +  N T +E       +Y + +F++ ++ F+TL  +++ NNW+ I EG  + S++   SR YF  F  V   ++ +IVAF+L+ F+   N   +    +++  I  +    K   ++  E+ R++R   +D T   +++S     +  ++++ S ++     + +R+   +S  M+QEEI+
Sbjct:  261 PYFSTLENSIVSLFVLLTTANFPDVMMPSYSRNPWSCVFFIVYLSIELYFIMNLLLAVVFDTFNDIEKRKFKSLLLHKRTAIQHAYRLL-----ISQRRPAG-------ISYRQFEGLM-RFYKPRMSARERYLTFKALNQNNTPLLSLKDFYDIYEVAALKWKAKKNREHWFDELPRTALLIFKG-INILVKSKAFQYFMYLVVAVNGVWILVETFMLKGGNFFSKHVPWSYLVFLTIYGVELFLKVAGLGPVEYLSSGWNLFDFSVTVFAFL--GLLALALNMEPFYFIVVLRPLQLLRLFKLK-ERYRNVLDTMFELLPRMASLGLTLLIFYYSFAIVGMEFFCGIVFPNCCNTSTVADAYRWR--------NHTVGNRTVVEEG-----YYYLNNFDNILNSFVTLFELTVVNNWYIIMEG--VTSQTSHWSRLYFMTFYIVTMVVMTIIVAFILEAFVFRMNYSRKNQDSEVDGGITLEKEISKEELVAVLELYREARGASSDVTRLLETLS-----QMERYQQHSMVFL---GRRSRTKSDLSLKMYQEEIQ 778
[Close]
BLAST of two pore calcium channel protein 1 vs. Ensembl Human
Match: TPCN1 (two pore segment channel 1 [Source:HGNC Symbol;Acc:HGNC:18182])

HSP 1 Score: 87.0409 bits (214), Expect = 7.059e-17
Identity = 125/558 (22.40%), Postives = 258/558 (46.24%), Query Frame = 1 
Query:  706 PYMQNFFDIVWETLIFITSSNSPDVILPGYSKSSYIGILFLIFYAM-FYFLLIFVYGLIMGNFRYHTKEEMTKLLVGKISLMYDAFVLLAEKIVINEKKNCNELFEKNIIKKETWKNLIARFKKYQNCEEAEEIMYSLLSQTNKTYLMKNEFLQMADLMSIPLTIVTNKFHFFE---KNFWFVFSNKLADFVESKYFNLIVGIVIVINSCCI---TFTSE---------PYXXXXXXXXXXXXXXXRIYVKGFIRYFHSLWNSFDFVVIVLAFIFWFVENMKLNVQTITFTXXXXXXXXXXXXXXGIAAYHQLLHILGMLIPTISSL-ITIV-------------FCFIVFYVCIGIWSYQDMFLMNRSELDYMFNSSSCNSTKLEGSDFGRQHYCILSFNSFISGFLTLCSVSIGNNWHTITEGYELESESMILSRFYFSVFKGVLTYLLLLIVAFVLDVFLLAYN---QKSSLKIEELIKNKLNSLKINFISYGEI-RKSRLTKTDSTEGSQSISLTRYFKRVKFEETSRIWFRLKAKGARSVEYVSQAMFQEEIE 2277
            PY     + +    + +T++N PDV++P YS++ +  + F+++ ++  YF++  +  ++   F    K +   LL+ K + +  A+ LL     I++++          I    ++ L+ RF K +       + +  L+Q N   L   +F  + ++ ++      N+ H+F+   +    +F   +   V+SK F   + +V+ +N   I   TF  +         P++ L F  ++ +E+ L++   G + Y  S WN FDF V V AF+   +  + LN++   F +++     L         Y  +L  +  L+P ++SL +T++             FC IVF  C    +  D +           N +  N T +E       +Y + +F++ ++ F+TL  +++ NNW+ I EG  + S++   SR YF  F  V   ++ +IVAF+L+ F+   N   +    +++  I  +    K   ++  E+ R++R   +D T   +++S     +  ++++ S ++     + +R+   +S  M+QEEI+
Sbjct:  333 PYFSTLENSIVSLFVLLTTANFPDVMMPSYSRNPWSCVFFIVYLSIELYFIMNLLLAVVFDTFNDIEKRKFKSLLLHKRTAIQHAYRLL-----ISQRRPAG-------ISYRQFEGLM-RFYKPRMSARERYLTFKALNQNNTPLLSLKDFYDIYEVAALKWKAKKNREHWFDELPRTALLIFKG-INILVKSKAFQYFMYLVVAVNGVWILVETFMLKGGNFFSKHVPWSYLVFLTIYGVELFLKVAGLGPVEYLSSGWNLFDFSVTVFAFL--GLLALALNMEPFYFIVVLRPLQLLRLFKLK-ERYRNVLDTMFELLPRMASLGLTLLIFYYSFAIVGMEFFCGIVFPNCCNTSTVADAYRWR--------NHTVGNRTVVE-----EGYYYLNNFDNILNSFVTLFELTVVNNWYIIMEG--VTSQTSHWSRLYFMTFYIVTMVVMTIIVAFILEAFVFRMNYSRKNQDSEVDGGITLEKEISKEELVAVLELYREARGASSDVTRLLETLS-----QMERYQQHSMVFL---GRRSRTKSDLSLKMYQEEIQ 850
[Close]
BLAST of two pore calcium channel protein 1 vs. Ensembl Human
Match: TPCN1 (two pore segment channel 1 [Source:HGNC Symbol;Acc:HGNC:18182])

HSP 1 Score: 87.0409 bits (214), Expect = 7.059e-17
Identity = 125/558 (22.40%), Postives = 258/558 (46.24%), Query Frame = 1 
Query:  706 PYMQNFFDIVWETLIFITSSNSPDVILPGYSKSSYIGILFLIFYAM-FYFLLIFVYGLIMGNFRYHTKEEMTKLLVGKISLMYDAFVLLAEKIVINEKKNCNELFEKNIIKKETWKNLIARFKKYQNCEEAEEIMYSLLSQTNKTYLMKNEFLQMADLMSIPLTIVTNKFHFFE---KNFWFVFSNKLADFVESKYFNLIVGIVIVINSCCI---TFTSE---------PYXXXXXXXXXXXXXXXRIYVKGFIRYFHSLWNSFDFVVIVLAFIFWFVENMKLNVQTITFTXXXXXXXXXXXXXXGIAAYHQLLHILGMLIPTISSL-ITIV-------------FCFIVFYVCIGIWSYQDMFLMNRSELDYMFNSSSCNSTKLEGSDFGRQHYCILSFNSFISGFLTLCSVSIGNNWHTITEGYELESESMILSRFYFSVFKGVLTYLLLLIVAFVLDVFLLAYN---QKSSLKIEELIKNKLNSLKINFISYGEI-RKSRLTKTDSTEGSQSISLTRYFKRVKFEETSRIWFRLKAKGARSVEYVSQAMFQEEIE 2277
            PY     + +    + +T++N PDV++P YS++ +  + F+++ ++  YF++  +  ++   F    K +   LL+ K + +  A+ LL     I++++          I    ++ L+ RF K +       + +  L+Q N   L   +F  + ++ ++      N+ H+F+   +    +F   +   V+SK F   + +V+ +N   I   TF  +         P++ L F  ++ +E+ L++   G + Y  S WN FDF V V AF+   +  + LN++   F +++     L         Y  +L  +  L+P ++SL +T++             FC IVF  C    +  D +           N +  N T +E       +Y + +F++ ++ F+TL  +++ NNW+ I EG  + S++   SR YF  F  V   ++ +IVAF+L+ F+   N   +    +++  I  +    K   ++  E+ R++R   +D T   +++S     +  ++++ S ++     + +R+   +S  M+QEEI+
Sbjct:  333 PYFSTLENSIVSLFVLLTTANFPDVMMPSYSRNPWSCVFFIVYLSIELYFIMNLLLAVVFDTFNDIEKRKFKSLLLHKRTAIQHAYRLL-----ISQRRPAG-------ISYRQFEGLM-RFYKPRMSARERYLTFKALNQNNTPLLSLKDFYDIYEVAALKWKAKKNREHWFDELPRTALLIFKG-INILVKSKAFQYFMYLVVAVNGVWILVETFMLKGGNFFSKHVPWSYLVFLTIYGVELFLKVAGLGPVEYLSSGWNLFDFSVTVFAFL--GLLALALNMEPFYFIVVLRPLQLLRLFKLK-ERYRNVLDTMFELLPRMASLGLTLLIFYYSFAIVGMEFFCGIVFPNCCNTSTVADAYRWR--------NHTVGNRTVVE-----EGYYYLNNFDNILNSFVTLFELTVVNNWYIIMEG--VTSQTSHWSRLYFMTFYIVTMVVMTIIVAFILEAFVFRMNYSRKNQDSEVDGGITLEKEISKEELVAVLELYREARGASSDVTRLLETLS-----QMERYQQHSMVFL---GRRSRTKSDLSLKMYQEEIQ 850
[Close]
BLAST of two pore calcium channel protein 1 vs. Ensembl Zebrafish
Match: tpcn3 (two pore segment channel 3 [Source:ZFIN;Acc:ZDB-GENE-141215-16])

HSP 1 Score: 150.984 bits (380), Expect = 2.912e-37
Identity = 203/779 (26.06%), Postives = 364/779 (46.73%), Query Frame = 1 
Query:  121 DLAFQLISDASVGRLTNFERTVTCLKYYRIY---LSRTFRFIRLIIHLTLVILLMYDASNKDSLSLIAKITIPIECACLSFYAIQLFIVYKFSIKSQFIKDKKHYCXXXXXXXXXXXXXXF----KSRVYQFRISPILRVLLFINSAEQSQXXXXXXXXXXXXXNVLVILPFLITFYFMMVAFIYRYFKINKSEPVFYYNKRPYMQNFFDIVWETLIFITSSNSPDVILPGYSKSSYIGILFLIFYAM-FYFLLIFVYGLIMGNFRYHTKEEMTKLLVGKISLMYDAFVLLAEKIVINEKKNCNELFEKNIIKKETWKNLIARFKKYQNCEEAEEIMYSLLSQTNKTYLMKNEFLQMADLMSIPLTIVTNKFHFFEKNFWFVFSNKLADF----VESKYFNLIVGIVIVINSCCITFTSEPYXXXXXXXXXXXXXXXRIYVKGFI---RYF---HSLWNSFDFVVIVLAFIFWFVENMKL---------NVQTITFTXXXXXXXXXXXXXXGIAAYHQLLHILGMLIPTISSLITIVFCFIVFYVCIGIWSYQ---DMFLMNRSELDYMFNSSSCNSTKLEGSDFGRQHYCILSFNSFISGFLTLCSVSIGNNWHTITEGYELESESMILSRF-YFSVFKGVLTYLLLLIVAFVLDVFLLAYN-QKSSL------KIEELIKN-KLNSLKINFISYGEIRKSRLTKTDSTEGSQSISLTRYFKRVKFEETSRIWFRLKAKGARSVEYVSQAMFQEEIEADLKKMKIYEHYFDS**NPNF 2340
            DLA   +SDA   R   F+ +   +K Y +Y     +T  ++ +II+L L +       +   + L    T  IE  CLS + +++    K   K +F KD K+ C  + + +S ID++++     +  Y  R S +LR LL +N  E  Q+RR  R++R  +  +  +  FL  F F ++ F     K+     +   N  PY  ++ DIV++  + +T++NSPDV++P Y+ S Y  I F+++  +  Y  + F   ++  N++ + KEE+ +L+  K   M  AF LL E        N  E  E  ++ +  W +L+ +  K +      E+++S+L   NK ++ K  F+Q+ADL+SI +  V ++ H  +  F  ++++  + F    V  + F     ++I++N+  I    E          FL   +L I +K +    R F   H  WN FD +++V A +F  + N  L          V  I F   +LR LRL++++  I  +  +++ L  + PTI +   ++      +  +G+  ++     F  N +  D  +    C +  L+ + F + +YC  +FN  IS F+ L  +++ N WH +T G+   + + + +R  +      V+  ++ + VAF+L+ FL+ Y   KS L      KIEEL  N + + +    +   E   S L    S +G +  SL                F++ ++ +R+V+ + Q MF    E DL+     E   D   N NF
Sbjct:   39 DLATVYVSDAKYNRNIFFDTSPQAVKLYLLYNHWFMQTLVYVFIIINLALALF-----EDPAVVPLPIWATSTIETICLSAFTVRIIHYAKVIPKDKFWKDPKNICIIIIVTLSFIDMVIYGALKATGHYGIRWSRVLRPLLLVNVTEGRQLRRAFRSIRNALPQISYVF-FL--FMFSVLVFSLMALKLFGKRGLLTINGSPYFTDYMDIVFDLYVLVTTANSPDVMMPAYNSSVYFTIFFILYIVINTYTFMSFFLAVVYNNYKKYLKEEVRQLVKAKRIKMCRAFSLLQE--------NRGEGGEP-VVTQANWNHLV-KLVKPKISTAHRELLWSVLDDQNKGHIGKFAFVQLADLLSIQVITVKSQAHPIQICFPSLYNSLPSRFIRQMVHHRVFVYAYDLIILVNAVFIGLDEENPVVSNAEWGFLALYMLEILLKLYATEPRAFFARHQFWNWFDTIIVVSA-LFGTIINSALKHSGGYTSRQVLDIVF---ILRVLRLIRVVDSIKRFRAIINTLIKIGPTILTFGQLILVVYYIFAMVGMELFKGKIQFFEPNSTSPDREY----CGNPLLKSTSFAKLNYCKNNFNDVISSFILLLELTVVNQWHVLTSGF--TAVTHVSARLFFVIFHIVVVIIIINIFVAFILEAFLVEYTVDKSELQTSLEKKIEELELNVQQDGVDTGLVDAMETNDSDLGS--SEDGKRKPSLM---------------FKIASRRSRTVDGLLQRMF----ETDLRPEDFNEEELD---NTNF 765
[Close]
BLAST of two pore calcium channel protein 1 vs. Ensembl Zebrafish
Match: tpcn1 (two pore segment channel 1 [Source:ZFIN;Acc:ZDB-GENE-060526-199])

HSP 1 Score: 85.1149 bits (209), Expect = 1.670e-16
Identity = 102/451 (22.62%), Postives = 207/451 (45.90%), Query Frame = 1 
Query:  748 IFITSSNSPDVILPGYSKSSYIGILFLIFYAM-FYFLLIFVYGLIMGNFRYHTKEEMTKLLVGKISLMYDAFVLLAEKIVINEKKNCNELFEKNIIKKETWKNLIARFKKYQNCEEAEEIMYSLLSQTNKTYLMKNEFLQMADLMSIPLTIVTNKFHFFE---KNFWFVFSNKLADFVESKYFNLIVGIVIVINSCCITFTSE------------PYXXXXXXXXXXXXXXXRIYVKGFIRYFHSLWNSFDFVVIVLAFIFWFVENMKLNVQTITFTXXXXXXXXXXXXXXGIAAYHQLLHILGMLIPTISSLITIVFCFIVFYVCIGIWSYQDMFLMNRSELDYMFNS-SSCNSTKLEGSDFGRQHYCILSFNSFISGFLTLCSVSIGNNWHTITEGYELESESMILSRFYFSVFKGVLTYLLLLIVAFVLD--VFLLAYNQKSSLKIEE 2043
            + +T++N PDV++P YSK+ +  I F+++ ++  YF++  +  ++   F    K +   LL+ K S +  AF LL  +               + +  + +  L+ RF   +       + +  L+ +    L   +F +  +++ +      +  H+F+   +    +F   +   V+SK F   + +V+ +N   I   +             P++ + F  ++ +E++L+I   G I YF S WN FDF V V AF+      +  +++   F +++     L         Y  +L  +  L P ++SL   +  F   +  +G+  + D+   N  +   +  S    N T    ++    +Y + +FN+ +S F+TL  +++ NNW+   EG  + S++   SR YF  F  V   ++ +IVAF+LD  VF + Y++K+   +++
Sbjct:  266 VLLTTANFPDVMMPAYSKNRWSCIFFIVYLSIELYFIMNLLLAVVFDTFNGVEKMKFKSLLLHKRSAIEHAFQLLVSRQ------------RPDGVSLKQFDGLM-RFYHPRMTPRDRFLTFKALNHSGAPMLSLQDFYKFYEVIGLKWKARRSGEHWFDDLPRTALLIFKG-INLLVKSKAFQYAMYLVVAVNGIWILVETNMLNDGISWTRVVPWSYIIFLTIYGIEVLLKITGLGPITYFSSGWNLFDFSVTVFAFLGLIA--LAFDMEPFYFIVVLRPLQLLRLFKIK-QRYRNVLDTMFELFPRMASLGLTLIIFYYSFAIVGMEFFADVIYPNCCDNSTVAASYRQINVTTGNRTELQEGYYYLNNFNNILSSFVTLFELTVVNNWYITMEG--VTSQTTHWSRLYFMTFYIVTMVVMTIIVAFILDAFVFRMNYSRKNREPLDD 697
[Close]
BLAST of two pore calcium channel protein 1 vs. Ensembl Zebrafish
Match: tpcn1 (two pore segment channel 1 [Source:ZFIN;Acc:ZDB-GENE-060526-199])

HSP 1 Score: 77.411 bits (189), Expect = 4.478e-14
Identity = 100/442 (22.62%), Postives = 195/442 (44.12%), Query Frame = 1 
Query:  748 IFITSSNSPDVILPGYSKSSYIGILFLIFYAM-FYFLLIFVYGLIMGNFRYHTKEEMTKLLVGKISLMYDAFVLLAEKIVINEKKNCNELFEKNIIKKETWKNLIARFKKYQNCEEAEEIMYSLLSQTNKTYLMKNEFLQMADLMSIPLTIVTNKFHFFEKNFWFVFSNKLADFVESKYFNLIVGIVIVINS------C--CITFTS---------EPYXXXXXXXXXXXXXXXRIYVKGFIRYFHSLWNSFDFVVIVLAFIFWFVENMKLNVQTITFTXXXXXXXXXXXXXXGIAAYHQLLHILGMLIPTISSLITIVFCFIVFYVCIGIWSYQDMFLMNRSELDYMFNS-SSCNSTKLEGSDFGRQHYCILSFNSFISGFLTLCSVSIGNNWHTITEGYELESESMILSRFYFSVFKGVLTYLLLLIVAFVLDVFLLAYN 2016
            + +T++N PDV++P YSK+ +  I F+++ ++  YF++  +  ++   F    K +   LL+ K S +  AF LL  +               + +  + +  L+ RF   +       + +  L+ +    L   +F +  +++ +      +  H      WF    + A  +       + GI I++ +      C  C+  T           P++ + F  ++ +E++L+I   G I YF S WN FDF V V AF+      +  +++   F +++     L         Y  +L  +  L P ++SL   +  F   +  +G+  + D+   N  +   +  S    N T    ++    +Y + +FN+ +S F+TL  +++ NNW+   EG  + S++   SR YF  F  V   ++ +IVAF+LD F+   N
Sbjct:  256 VLLTTANFPDVMMPAYSKNRWSCIFFIVYLSIELYFIMNLLLAVVFDTFNGVEKMKFKSLLLHKRSAIEHAFQLLVSRQ------------RPDGVSLKQFDGLM-RFYHPRMTPRDRFLTFKALNHSGAPMLSLQDFYKFYEVIGLKWKARRSGEH------WFDDLPRTALLIFKDLVVAVNGIWILVETNMLNGNCFLCVKPTELDGISWTRVVPWSYIIFLTIYGIEVLLKITGLGPITYFSSGWNLFDFSVTVFAFLGLIA--LAFDMEPFYFIVVLRPLQLLRLFKIK-QRYRNVLDTMFELFPRMASLGLTLIIFYYSFAIVGMEFFADVIYPNCCDNSTVAASYRQINVTTGNRTELQEGYYYLNNFNNILSSFVTLFELTVVNNWYITMEG--VTSQTTHWSRLYFMTFYIVTMVVMTIIVAFILDAFVFRMN 673
[Close]
BLAST of two pore calcium channel protein 1 vs. Ensembl Zebrafish
Match: tpcn1 (two pore segment channel 1 [Source:ZFIN;Acc:ZDB-GENE-060526-199])

HSP 1 Score: 62.7734 bits (151), Expect = 1.536e-9
Identity = 56/203 (27.59%), Postives = 96/203 (47.29%), Query Frame = 1 
Query: 1411 GFIRYFHSLWNSFDFVVIVLAFIFWFVENMKLNVQTITFTXXXXXXXXXXXXXXGIAAYHQLLHILGMLIPTISSLITIVFCFIVFYVCIGIWSYQDMFLMNRSELDYMFNS-SSCNSTKLEGSDFGRQHYCILSFNSFISGFLTLCSVSIGNNWHTITEGYELESESMILSRFYFSVFKGVLTYLLLLIVAFVLDVFLLAYN 2016
            G I YF S WN FDF V V AF+      +  +++   F +++     L         Y  +L  +  L P ++SL   +  F   +  +G+  + D+   N  +   +  S    N T    ++    +Y + +FN+ +S F+TL  +++ NNW+   EG  + S++   SR YF  F  V   ++ +IVAF+LD F+   N
Sbjct:  466 GPITYFSSGWNLFDFSVTVFAFLGLIA--LAFDMEPFYFIVVLRPLQLLRLFKIK-QRYRNVLDTMFELFPRMASLGLTLIIFYYSFAIVGMEFFADVIYPNCCDNSTVAASYRQINVTTGNRTELQEGYYYLNNFNNILSSFVTLFELTVVNNWYITMEG--VTSQTTHWSRLYFMTFYIVTMVVMTIIVAFILDAFVFRMN 663
[Close]
BLAST of two pore calcium channel protein 1 vs. Ensembl Xenopus
Match: ENSXETT00000032923.1 (two pore calcium channel protein 1-like [Source:NCBI gene;Acc:100495031])

HSP 1 Score: 139.043 bits (349), Expect = 2.607e-33
Identity = 184/764 (24.08%), Postives = 359/764 (46.99%), Query Frame = 1 
Query:  106 SQDTLDLAFQLISDASVGRLTNFERTVTCLKYYRIYLSRTFRFIRLIIHLTLVILLMYDASNKDSLSLIAKITIPIECACLSFYAIQLFIVYKFSIKSQFIKDKKHYCXXXXXXXXXXXXXXFKS----RVYQFRISPILRVLLFINSAEQSQXXXXXXXXXXXXXNVL---VILPFLITFYFMMVAFIYRYFKINKSEPVFYYNKRPYMQNFFDIVWETLIFITSSNSPDVILPGYSKSSYIGILFLIFYAMFYFLLIFVY-GLIMGNFRYHTKEEMTKLLVGKISLMYDAFVLLAEKIVINEKKNCNELFEKNIIKKETWKNLIARFKKYQNCEEAEEIMYSLLSQTNKTYLMKNEFLQMADLMSIPLTIVTNKFHFFEKNFWFVFSNKLADF----VESKYFNLIVGIVIVINSCCITFTSEPYXXXXXXXXXXXXXXXRIYVKGFI---RYF---HSLWNSFDFVVIVLAFIFWFVENMKLNV-----QTITFTXXXXXXXXXXXXXXGIAAYHQLLHILGMLIPTISS----LITIVFCFIVFYVCIGIWSYQDMFLMNRSELDYMFNSSSCNSTKLEGSDFGRQHYCILSFNSFISGFLTLCSVSIGNNWHTITEGYELESESMILSRFYFSVFK-GVLTYLLLLIVAFVLDVFLLAYNQKSSLKIEELIKNKLNSLKINFISYGEIRKSRLTKTDSTEGSQSISLTRYFKRVKFEETSR--IWFRLKAKGARSVEYVSQAMFQEEIEADLKKMKIYE 2307
             +  L LA   + DA   R  +FE +   ++ Y  Y     +          ++L +++      L  +A  T  +E  CL  +  +L    K + +S F KD K+ C  ++I+++LID+I++ +     +   R S +LR +  IN AE  QIRR  R++R T+  ++   ++  F +  + +M   ++   K+  +E        PY  N+ D+V+   + +T++NSPDV++P Y  + +  I F+ +  +  ++ + V+  ++  N+R H K E+ KL   K   M DAF  L       + K  +E     +IK++ WK L+ +           E++  +       Y+ K+ F+++ADL++I +  +  + H  E     V+ +  + F    V+ K F      +I++N+  I              +FL   ++ I +K +    R F   +  WN FD ++IV A I   +     +V     Q +   + +LR LRL++++  I  +  +++ L  ++PT+ +    LI I + F +  + + I++ +  F    S   Y   +  C ++ L+ S F +  YC  +FN   S F+ L  +++ N WH +  G+ L + +   +R YF  F   ++  ++ + VAF+L+ F + Y+ + S ++E  I+ K+  L +      +   S     ++ E  ++ + T       F ET++  + FR+ +K  R+V+ + Q MF+ EI  + +   I +
Sbjct:   35 DEQNLLLAAAFVYDAQYNRNIHFETSPEAVRLYYFYNHWIMQVATYFFITVDLLLAVFEEPAVYPLPFLA--TSIVEVLCLLAFFGRLVHFSKVTPRSIFWKDTKNICVMVTIVLNLIDLIIYGALIIAEIQSVRWSRVLRPIYLINFAESRQIRRAFRSIRNTLPEIMYVFLLFMFSVLLFSLMALQLFGNRKLKTAE------GEPYFANYLDVVFNLYVLVTTANSPDVMMPAYDHNPWYSIFFIAYIILNTYIFMSVFLAVVYNNYRKHLKNEIRKLAYMKRRKMVDAFNAL-------KVKEGSEF----VIKEDKWKQLV-KLVAPDISNSHRELLLRVSDDEQNGYVGKDAFIRLADLLNIKVITMKTRMHPLESWMPHVYKSAWSRFIRRVVQHKGFVYAYDGIILVNAIFIALDENNPLISNAEWVFLAFYLVEILLKLYTYDPRSFFAKNQFWNWFDTLIIVAALIATIINTSLKSVGQYSSQQVLDIVFILRVLRLIRIIDSIQRFRVIMNTLINILPTMLTFSGLLIAIYYVFAI--LGMEIFNGKIRFFPENSTDPY---AQECGTSALKDSAFAKSKYCKNNFNDLGSAFIVLVELTVVNQWHVLANGFSLVTHAA--ARLYFIAFHVVIVILIINIFVAFILEAFFVEYSLEKS-EVETAIEKKIQELGMAV----QEDASSGNLVENMESCETDAGT-------FGETNQKGLMFRIASKRYRTVDALLQRMFESEIPPEDEGPPIED 759
[Close]
BLAST of two pore calcium channel protein 1 vs. Ensembl Xenopus
Match: ENSXETT00000018477.1 (two pore calcium channel protein 1-like [Source:NCBI gene;Acc:100495031])

HSP 1 Score: 136.346 bits (342), Expect = 1.721e-32
Identity = 183/755 (24.24%), Postives = 352/755 (46.62%), Query Frame = 1 
Query:  109 QDTLDLAFQLISDASVGRLTNFERTVTCLKYYRIYLSRTFRFIRLIIHLTLVILLMYDASNKDSLSLIAKITIPIECACLSFYAIQLFIVYKFSIKSQFIKDKKHYCXXXXXXXXXXXXXXFKS----RVYQFRISPILRVLLFINSAEQSQXXXXXXXXXXXXXNVL---VILPFLITFYFMMVAFIYRYFKINKSEPVFYYNKRPYMQNFFDIVWETLIFITSSNSPDVILPGYSKSSYIGILFLIFYAMFYFLLIFVY-GLIMGNFRYHTKEEMTKLLVGKISLMYDAFVLLAEKIVINEKKNCNELFEKNIIKKETWKNLIARFKKYQNCEEAEEIMYSLLSQTNKTYLMKNEFLQMADLMSIPLTIVTNKFHFFEKNFWFVFSNKLADF----VESKYFNLIVGIVIVINSCCITFTSEPYXXXXXXXXXXXXXXXRIYVKGFI---RYF---HSLWNSFDFVVIVLAFIFWFVENMKLNV-----QTITFTXXXXXXXXXXXXXXGIAAYHQLLHILGMLIPTISS----LITIVFCFIVFYVCIGIWSYQDMFLMNRSELDYMFNSSSCNSTKLEGSDFGRQHYCILSFNSFISGFLTLCSVSIGNNWHTITEGYELESESMILSRFYFSVFK-GVLTYLLLLIVAFVLDVFLLAYNQKSSLKIEELIKNKLNSLKINFISYGEIRKSRLTKTDSTEGSQSISLTRYFKRVKFEETSR--IWFRLKAKGARSVEYVSQAMFQEEIEAD 2283
            Q  L LA   + DA   R  +FE +   ++ Y  Y     +          ++L +++      L  +A  T  +E  CL  +  +L    K + +S F KD K+ C  ++I+++LID+I++ +     +   R S +LR +  IN AE  QIRR  R++R T+  ++   ++  F +  + +M   ++   K+  +E        PY  N+ D+V+   + +T++NSPDV++P Y  + +  I F+ +  +  ++ + V+  ++  N+R H K E+ KL   K   M DAF  L       + K  +E     +IK++ WK L+ +           E++  +       Y+ K+ F+++ADL++I +  +  + H  E     V+ +  + F    V+ K F      +I++N+  I              +FL   ++ I +K +    R F   +  WN FD ++IV A I   +     +V     Q +   + +LR LRL++++  I  +  +++ L  ++PT+ +    LI I + F +  + + I++ +  F    S   Y   +  C ++ L+ S F +  YC  +FN   S F+ L  +++ N WH I          +  +R YF  F   ++  ++ + VAF+L+ F + Y+ + S ++E  I+ K+  L    ++  EI+   +   ++  G+             F ET++  + FR+ +K  R+V+ + Q MF+ EI  +
Sbjct:    1 QQNLLLAAAFVYDAQYNRNIHFETSPEAVRLYYFYNHWIMQVATYFFITVDLLLAVFEEPAVYPLPFLA--TSIVEVLCLLAFFGRLVHFSKVTPRSIFWKDTKNICVMVTIVLNLIDLIIYGALIIAEIQSVRWSRVLRPIYLINFAESRQIRRAFRSIRNTLPEIMYVFLLFMFSVLLFSLMALQLFGNRKLKTAE------GEPYFANYLDVVFNLYVLVTTANSPDVMMPAYDHNPWYSIFFIAYIILNTYIFMSVFLAVVYNNYRKHLKNEIRKLAYMKRRKMVDAFNAL-------KVKEGSEF----VIKEDKWKQLV-KLVAPDISNSHRELLLRVSDDEQNGYVGKDAFIRLADLLNIKVITMKTRMHPLESWMPHVYKSAWSRFIRRVVQHKGFVYAYDGIILVNAIFIALDENNPLISNAEWVFLAFYLVEILLKLYTYDPRSFFAKNQFWNWFDTLIIVAALIATIINTSLKSVGQYSSQQVLDIVFILRVLRLIRIIDSIQRFRVIMNTLINILPTMLTFSGLLIAIYYVFAI--LGMEIFNGKIRFFPENSTDPY---AQECGTSALKDSAFAKSKYCKNNFNDLGSAFIVLVELTVVNQWHDIL------YLKICAARLYFIAFHVVIVILIINIFVAFILEAFFVEYSLEKS-EVETAIEKKIQELG---MAVQEIQNHNMESCETDAGT-------------FGETNQKGLMFRIASKRYRTVDALLQRMFESEIPPE 707
[Close]
BLAST of two pore calcium channel protein 1 vs. Ensembl Xenopus
Match: ENSXETT00000032877.1 (two pore calcium channel protein 1-like [Source:NCBI gene;Acc:100495031])

HSP 1 Score: 132.88 bits (333), Expect = 2.129e-31
Identity = 182/747 (24.36%), Postives = 355/747 (47.52%), Query Frame = 1 
Query:  124 LAFQLISDASVGRLTNFERTVTCLKYYRIYLSRTFRFIRLIIHLTLVILLMYDASNKDSLSLIAKITIPIECACLSFYAIQLFIVYKFSIKSQFIKDKKHYCXXXXXXXXXXXXXXFKS----RVYQFRISPILRVLLFINSAEQSQXXXXXXXXXXXXXNVL---VILPFLITFYFMMVAFIYRYFKINKSEPVFYYNKRPYMQNFFDIVWETLIFITSSNSPDVILPGYSKSSYIGILFLIFYAMFYFLLIFVY-GLIMGNFRYHTKEEMTKLLVGKISLMYDAFVLLAEKIVINEKKNCNELFEKNIIKKETWKNLIARFKKYQNCEEAEEIMYSLLSQTNKTYLMKNEFLQMADLMSIPLTIVTNKFHFFEKNFWFVFSNKLADF----VESKYFNLIVGIVIVINSCCITFTSEPYXXXXXXXXXXXXXXXRIYVKGFI---RYF---HSLWNSFDFVVIVLAFIFWFVE-NMKLNV-QTITFTXXXXXXXXXXXXXXGIAAYHQLLHILGMLIPTISS----LITIVFCFIVFYVCIGIWSYQDMFLMNRSELDYMFNSSSCNSTKLEGSDFGRQHYCILSFNSFISGFLTLCSVSIGNNWHTITEGYELESESMILSRFYFSVFK-GVLTYLLLLIVAFVLDVFLLAYNQKSSLKIEELIKNKLNSLKINFISYGEIRKSRLTKTDSTEGSQSISLTRYFKRVKFEETSR--IWFRLKAKGARSVEYVSQAMFQEEIEAD 2283
            LA   + DA   R  +FE +   ++ Y  Y     +          ++L +++      L  +A  T  +E  CL  +  +L    K + +S F KD K+ C  ++I+++LID+I++ +     +   R S +LR +  IN AE  QIRR  R++R T+  ++   ++  F +  + +M   ++   K+  +E        PY  N+ D+V+   + +T++NSPDV++P Y  + +  I F+ +  +  ++ + V+  ++  N+R H K E+ KL   K   M DAF  L       + K  +E     +IK++ WK L+ +           E++  +       Y+ K+ F+++ADL++I +  +  + H  E     V+ +  + F    V+ K F      +I++N+  I              +FL   ++ I +K +    R F   +  WN FD ++IV A I   +  ++K  + Q +   + +LR LRL++++  I  +  +++ L  ++PT+ +    LI I + F +  + + I++ +  F    S   Y   +  C ++ L+ S F +  YC  +FN   S F+ L  +++ N WH I          +  +R YF  F   ++  ++ + VAF+L+ F + Y+ + S ++E  I+ K+  L    ++  EI+   +   ++ E  ++ + T       F ET++  + FR+ +K  R+V+ + Q MF+ EI  +
Sbjct:   29 LAAAFVYDAQYNRNIHFETSPEAVRLYYFYNHWIMQVATYFFITVDLLLAVFEEPAVYPLPFLA--TSIVEVLCLLAFFGRLVHFSKVTPRSIFWKDTKNICVMVTIVLNLIDLIIYGALIIAEIQSVRWSRVLRPIYLINFAESRQIRRAFRSIRNTLPEIMYVFLLFMFSVLLFSLMALQLFGNRKLKTAE------GEPYFANYLDVVFNLYVLVTTANSPDVMMPAYDHNPWYSIFFIAYIILNTYIFMSVFLAVVYNNYRKHLKNEIRKLAYMKRRKMVDAFNAL-------KVKEGSEF----VIKEDKWKQLV-KLVAPDISNSHRELLLRVSDDEQNGYVGKDAFIRLADLLNIKVITMKTRMHPLESWMPHVYKSAWSRFIRRVVQHKGFVYAYDGIILVNAIFIALDENNPLISNAEWVFLAFYLVEILLKLYTYDPRSFFAKNQFWNWFDTLIIVAALIATIINTSLKSGISQQVLDIVFILRVLRLIRIIDSIQRFRVIMNTLINILPTMLTFSGLLIAIYYVFAI--LGMEIFNGKIRFFPENSTDPY---AQECGTSALKDSAFAKSKYCKNNFNDLGSAFIVLVELTVVNQWHDIL------YLKICAARLYFIAFHVVIVILIINIFVAFILEAFFVEYSLEKS-EVETAIEKKIQELG---MAVQEIQNHAI--VENMESCETDAGT-------FGETNQKGLMFRIASKRYRTVDALLQRMFESEIPPE 731
[Close]
BLAST of two pore calcium channel protein 1 vs. Ensembl Xenopus
Match: ENSXETT00000032915.1 (two pore calcium channel protein 1-like [Source:NCBI gene;Acc:100495031])

HSP 1 Score: 106.301 bits (264), Expect = 4.372e-23
Identity = 148/614 (24.10%), Postives = 285/614 (46.42%), Query Frame = 1 
Query:  106 SQDTLDLAFQLISDASVGRLTNFERTVTCLKYYRIYLSRTFRFIRLIIHLTLVILLMYDASNKDSLSLIAKITIPIECACLSFYAIQLFIVYKFSIKSQFIKDKKHYCXXXXXXXXXXXXXXFKS----RVYQFRISPILRVLLFINSAEQSQXXXXXXXXXXXXXNVL---VILPFLITFYFMMVAFIYRYFKINKSEPVFYYNKRPYMQNFFDIVWETLIFITSSNSPDVILPGYSKSSYIGILFLIFYAMFYFLLIFVY-GLIMGNFRYHTKEEMTKLLVGKISLMYDAFVLLAEKIVINEKKNCNELFEKNIIKKETWKNLIARFKKYQNCEEAEEIMYSLLSQTNKTYLMKNEFLQMADLMSIPLTIVTNKFHFFEKNFWFVFSNKLADF----VESKYFNLIVGIVIVINSCCITFTSEPYXXXXXXXXXXXXXXXRIYVKGFI---RYF---HSLWNSFDFVVIVLAFIFWFVENMKLNV-----QTITFTXXXXXXXXXXXXXXGIAAYHQLLHILGMLIPTISS----LITIVFCFIVFYVCIGIWSYQDMFLMNRSELDYMFNSSSCNSTKLEGSDFGRQHYCILSFNSFISGFLTLCSVSIGN 1866
             +  L LA   + DA   R  +FE +   ++ Y  Y     +          ++L +++      L  +A  T  +E  CL  +  +L    K + +S F KD K+ C  ++I+++LID+I++ +     +   R S +LR +  IN AE  QIRR  R++R T+  ++   ++  F +  + +M   ++   K+  +E        PY  N+ D+V+   + +T++NSPDV++P Y  + +  I F+ +  +  ++ + V+  ++  N+R H K E+ KL   K   M DAF  L       + K  +E     +IK++ WK L+ +           E++  +       Y+ K+ F+++ADL++I +  +  + H  E     V+ +  + F    V+ K F      +I++N+  I              +FL   ++ I +K +    R F   +  WN FD ++IV A I   +     +V     Q +   + +LR LRL++++  I  +  +++ L  ++PT+ +    LI I + F +  + + I++ +  F    S   Y   +  C ++ L+ S F +  YC  +FN   S F+ L  +++ N
Sbjct:   35 DEQNLLLAAAFVYDAQYNRNIHFETSPEAVRLYYFYNHWIMQVATYFFITVDLLLAVFEEPAVYPLPFLA--TSIVEVLCLLAFFGRLVHFSKVTPRSIFWKDTKNICVMVTIVLNLIDLIIYGALIIAEIQSVRWSRVLRPIYLINFAESRQIRRAFRSIRNTLPEIMYVFLLFMFSVLLFSLMALQLFGNRKLKTAE------GEPYFANYLDVVFNLYVLVTTANSPDVMMPAYDHNPWYSIFFIAYIILNTYIFMSVFLAVVYNNYRKHLKNEIRKLAYMKRRKMVDAFNAL-------KVKEGSEF----VIKEDKWKQLV-KLVAPDISNSHRELLLRVSDDEQNGYVGKDAFIRLADLLNIKVITMKTRMHPLESWMPHVYKSAWSRFIRRVVQHKGFVYAYDGIILVNAIFIALDENNPLISNAEWVFLAFYLVEILLKLYTYDPRSFFAKNQFWNWFDTLIIVAALIATIINTSLKSVGQYSSQQVLDIVFILRVLRLIRIIDSIQRFRVIMNTLINILPTMLTFSGLLIAIYYVFAI--LGMEIFNGKIRFFPENSTDPY---AQECGTSALKDSAFAKSKYCKNNFNDLGSAFIVLVELTVVN 623
[Close]
BLAST of two pore calcium channel protein 1 vs. Ensembl Xenopus
Match: ENSXETT00000032851.1 (two pore calcium channel protein 1-like [Source:NCBI gene;Acc:100495031])

HSP 1 Score: 105.916 bits (263), Expect = 4.492e-23
Identity = 134/544 (24.63%), Postives = 255/544 (46.88%), Query Frame = 1 
Query:  331 IECACLSFYAIQLFIVYKFSIKSQFIKDKKH----YCXXXXXXXXXXXXXXFKSRVYQFRISPILRVLLFINSAEQSQXXXXXXXXXXXXXNVL---VILPFLITFYFMMVAFIYRYFKINKSEPVFYYNKRPYMQNFFDIVWETLIFITSSNSPDVILPGYSKSSYIGILFLIFYAMFYFLLIFVY-GLIMGNFRYHTKEEMTKLLVGKISLMYDAFVLLAEKIVINEKKNCNELFEKNIIKKETWKNLIARFKKYQNCEEAEEIMYSLLSQTNKTYLMKNEFLQMADLMSIPLTIVTNKFHFFEKNFWFVFSNKLADF----VESKYFNLIVGIVIVINSCCITFTSEPYXXXXXXXXXXXXXXXRIYVKGFI---RYF---HSLWNSFDFVVIVLAFIFWFVENMKLNV-----QTITFTXXXXXXXXXXXXXXGIAAYHQLLHILGMLIPTISS----LITIVFCFIVFYVCIGIWSYQDMFLMNRSELDYMFNSSSCNSTKLEGSDFGRQHYCILSFNSFISGFLTLCSVSIGNNWHTI 1881
            +E  CL  +  +L    K + +S F KD K+    +   L+++  +I   L  + +   R S +LR +  IN AE  QIRR  R++R T+  ++   ++  F +  + +M   ++   K+  +E        PY  N+ D+V+   + +T++NSPDV++P Y  + +  I F+ +  +  ++ + V+  ++  N+R H K E+ KL   K   M DAF  L       + K  +E     +IK++ WK L+ +           E++  +       Y+ K+ F+++ADL++I +  +  + H  E     V+ +  + F    V+ K F      +I++N+  I              +FL   ++ I +K +    R F   +  WN FD ++IV A I   +     +V     Q +   + +LR LRL++++  I  +  +++ L  ++PT+ +    LI I + F +  + + I++ +  F    S   Y   +  C ++ L+ S F +  YC  +FN   S F+ L  +++ N WH I
Sbjct:   68 VEVLCLLAFFGRLVHFSKVTPRSIFWKDTKNIFHIFILQLNLIDLIIYGALIIAEIQSVRWSRVLRPIYLINFAESRQIRRAFRSIRNTLPEIMYVFLLFMFSVLLFSLMALQLFGNRKLKTAE------GEPYFANYLDVVFNLYVLVTTANSPDVMMPAYDHNPWYSIFFIAYIILNTYIFMSVFLAVVYNNYRKHLKNEIRKLAYMKRRKMVDAFNAL-------KVKEGSEF----VIKEDKWKQLV-KLVAPDISNSHRELLLRVSDDEQNGYVGKDAFIRLADLLNIKVITMKTRMHPLESWMPHVYKSAWSRFIRRVVQHKGFVYAYDGIILVNAIFIALDENNPLISNAEWVFLAFYLVEILLKLYTYDPRSFFAKNQFWNWFDTLIIVAALIATIINTSLKSVGQYSSQQVLDIVFILRVLRLIRIIDSIQRFRVIMNTLINILPTMLTFSGLLIAIYYVFAI--LGMEIFNGKIRFFPENSTDPY---AQECGTSALKDSAFAKSKYCKNNFNDLGSAFIVLVELTVVNQWHDI 588
[Close]
BLAST of two pore calcium channel protein 1 vs. Ensembl Mouse
Match: Tpcn1 (two pore channel 1 [Source:MGI Symbol;Acc:MGI:2182472])

HSP 1 Score: 103.219 bits (256), Expect = 3.678e-22
Identity = 126/551 (22.87%), Postives = 257/551 (46.64%), Query Frame = 1 
Query:  706 PYMQNFFDIVWETLIFITSSNSPDVILPGYSKSSYIGILFLIFYAM-FYFLLIFVYGLIMGNFRYHTKEEMTKLLVGKISLMYDAFVLLAEKIVINEKKNCNELFEKNIIKKETWKNLIARFKKYQNCEEAEEIMYSLLSQTNKTYLMKNEFLQMADLMSIPLTIVTNKFHFFEK--NFWFVFSNKLADFVESKYFNLIVGIVIVINSCCI----------TFTSE--PYXXXXXXXXXXXXXXXRIYVKGFIRYFHSLWNSFDFVVIVLAFIFWFVENMKLNVQTITFTXXXXXXXXXXXXXXGIAAYHQLLHILGMLIPTISSLITIVFCFIVFYVCIGIWSYQDMFLMNRSELD-----YMF-NSSSCNSTKLEGSDFGRQHYCILSFNSFISGFLTLCSVSIGNNWHTITEGYELESESMILSRFYFSVFKGVLTYLLLLIVAFVLDVFLLAYN-----QKSSLKIEELIKNKLNSLKINFISYGEI-RKSRLTKTDSTEGSQSISLTRYFKRVKFEETSRIWFRLKAKGARSVEYVSQAMFQEEIE 2277
            PY     + +    + +T++N PDV++P YS++ +  + F+++ ++  YF++  +  ++   F    K +   LL+ K + +  A+ LLA     ++++          I    ++ L+ RF K +       + +  L+Q+N   L   +F  + ++ ++      N+ H+F++     F+    +   V SK F   + +V+ +N   I           FTS+  P++ L F  ++ +E+ +++   G + Y  S WN FDF V   AF+      + LN++   F +++     L         Y  +L  +  L+P ++SL   +  F   +  +G+  +      N          Y F N +  N TK+E       +Y + +F++ ++ F+TL  +++ NNW+ I EG  + S++   SR YF  F  V   ++ +IVAF+L+ F+   N     Q S +    +I+ +++  K   ++  E+ R+ R T +D T    ++S     +  K+++ S ++     + +R+   +S  M+QEEI+
Sbjct:  262 PYFSTLENSIVNLFVLLTTANFPDVMMPSYSRNPWSCVFFIVYLSIELYFIMNLLLAVVFDTFNDIEKHKFKSLLLHKRTAIQHAYGLLA-----SQRRPAG-------ISYRQFEGLM-RFYKPRMSARERFLTFKALNQSNTPLLSLKDFYDIYEVAALQWKAKRNRQHWFDELPRTAFLIFKGINILVNSKAFQYFMYLVVAVNGVWILVETFMLKGGNFTSKHVPWSYLVFLTIYGVELFMKVAGLGPVEYLSSGWNLFDFSVTAFAFLGLLA--LTLNMEPFYFIVVLRPLQLLRLFKLK-KRYRNVLDTMFELLPRMASLGLTLLTFYYSFAIVGMEFFNGRLTPNCCNTSTVADAYRFINHTVGNKTKVE-----EGYYYLNNFDNILNSFVTLFELTVVNNWYIIMEG--VTSQTSHWSRLYFMTFYIVTMVVMTIIVAFILEAFVFRMNYSRKSQDSEVDSGIVIEKEMS--KEELMAVLELYREERGTSSDVTRLLDTLS-----QMEKYQQNSMVFL---GRRSRTKSDLSLKMYQEEIQ 779
[Close]
BLAST of two pore calcium channel protein 1 vs. UniProt/SwissProt
Match: sp|Q9EQJ0|TPC1_MOUSE (Two pore calcium channel protein 1 OS=Mus musculus OX=10090 GN=Tpcn1 PE=1 SV=1)

HSP 1 Score: 103.219 bits (256), Expect = 2.573e-21
Identity = 126/551 (22.87%), Postives = 257/551 (46.64%), Query Frame = 1 
Query:  706 PYMQNFFDIVWETLIFITSSNSPDVILPGYSKSSYIGILFLIFYAM-FYFLLIFVYGLIMGNFRYHTKEEMTKLLVGKISLMYDAFVLLAEKIVINEKKNCNELFEKNIIKKETWKNLIARFKKYQNCEEAEEIMYSLLSQTNKTYLMKNEFLQMADLMSIPLTIVTNKFHFFEK--NFWFVFSNKLADFVESKYFNLIVGIVIVINSCCI----------TFTSE--PYXXXXXXXXXXXXXXXRIYVKGFIRYFHSLWNSFDFVVIVLAFIFWFVENMKLNVQTITFTXXXXXXXXXXXXXXGIAAYHQLLHILGMLIPTISSLITIVFCFIVFYVCIGIWSYQDMFLMNRSELD-----YMF-NSSSCNSTKLEGSDFGRQHYCILSFNSFISGFLTLCSVSIGNNWHTITEGYELESESMILSRFYFSVFKGVLTYLLLLIVAFVLDVFLLAYN-----QKSSLKIEELIKNKLNSLKINFISYGEI-RKSRLTKTDSTEGSQSISLTRYFKRVKFEETSRIWFRLKAKGARSVEYVSQAMFQEEIE 2277
            PY     + +    + +T++N PDV++P YS++ +  + F+++ ++  YF++  +  ++   F    K +   LL+ K + +  A+ LLA     ++++          I    ++ L+ RF K +       + +  L+Q+N   L   +F  + ++ ++      N+ H+F++     F+    +   V SK F   + +V+ +N   I           FTS+  P++ L F  ++ +E+ +++   G + Y  S WN FDF V   AF+      + LN++   F +++     L         Y  +L  +  L+P ++SL   +  F   +  +G+  +      N          Y F N +  N TK+E       +Y + +F++ ++ F+TL  +++ NNW+ I EG  + S++   SR YF  F  V   ++ +IVAF+L+ F+   N     Q S +    +I+ +++  K   ++  E+ R+ R T +D T    ++S     +  K+++ S ++     + +R+   +S  M+QEEI+
Sbjct:  262 PYFSTLENSIVNLFVLLTTANFPDVMMPSYSRNPWSCVFFIVYLSIELYFIMNLLLAVVFDTFNDIEKHKFKSLLLHKRTAIQHAYGLLA-----SQRRPAG-------ISYRQFEGLM-RFYKPRMSARERFLTFKALNQSNTPLLSLKDFYDIYEVAALQWKAKRNRQHWFDELPRTAFLIFKGINILVNSKAFQYFMYLVVAVNGVWILVETFMLKGGNFTSKHVPWSYLVFLTIYGVELFMKVAGLGPVEYLSSGWNLFDFSVTAFAFLGLLA--LTLNMEPFYFIVVLRPLQLLRLFKLK-KRYRNVLDTMFELLPRMASLGLTLLTFYYSFAIVGMEFFNGRLTPNCCNTSTVADAYRFINHTVGNKTKVE-----EGYYYLNNFDNILNSFVTLFELTVVNNWYIIMEG--VTSQTSHWSRLYFMTFYIVTMVVMTIIVAFILEAFVFRMNYSRKSQDSEVDSGIVIEKEMS--KEELMAVLELYREERGTSSDVTRLLDTLS-----QMEKYQQNSMVFL---GRRSRTKSDLSLKMYQEEIQ 779
[Close]
BLAST of two pore calcium channel protein 1 vs. UniProt/SwissProt
Match: sp|Q9WTN5|TPC1_RAT (Two pore calcium channel protein 1 OS=Rattus norvegicus OX=10116 GN=Tpcn1 PE=1 SV=2)

HSP 1 Score: 100.523 bits (249), Expect = 2.256e-20
Identity = 125/551 (22.69%), Postives = 260/551 (47.19%), Query Frame = 1 
Query:  706 PYMQNFFDIVWETLIFITSSNSPDVILPGYSKSSYIGILFLIFYAM-FYFLLIFVYGLIMGNFRYHTKEEMTKLLVGKISLMYDAFVLLAEKIVINEKKNCNELFEKNIIKKETWKNLIARFKKYQNCEEAEEIMYSLLSQTNKTYLMKNEFLQMADLMSIPLTIVTNKFHFFEK--NFWFVFSNKLADFVESKYFNLIVGIVIVINSCCI----------TFTSE--PYXXXXXXXXXXXXXXXRIYVKGFIRYFHSLWNSFDFVVIVLAFIFWFVENMKLNVQTITFTXXXXXXXXXXXXXXGIAAYHQLLHILGMLIPTISSLITIVFCFIVFYVCIGIWSYQDMFLMN----RSELD-YMF-NSSSCNSTKLEGSDFGRQHYCILSFNSFISGFLTLCSVSIGNNWHTITEGYELESESMILSRFYFSVFKGVLTYLLLLIVAFVLDVFLLAYN-----QKSSLKIEELIKNKLNSLKINFISYGEI-RKSRLTKTDSTEGSQSISLTRYFKRVKFEETSRIWFRLKAKGARSVEYVSQAMFQEEIE 2277
            PY     + +    + +T++N PDV++P YS++ +  + F+++ ++  YF++  +  ++   F    K +   LL+ K + +  A+ LL     +++++          I    ++ L+ RF K +       + +  L+Q+N   L   +F  + ++ ++      N+ H+F++     F+    +   V SK F   + +V+ +N   I           F S+  P++ L F  ++ +E+ +++   G + Y  S WN FDF V   AF+      + LN++   F +++     L         Y  +L  +  L+P ++SL   +  F   +  +G+  +      N     +  D Y F N +  N TK+E       +Y + +F++ ++ F+TL  +++ NNW+ I EG  + S++   SR YF  F  V   ++ +IVAF+L+ F+   N     Q+S +    +I+ +++  K   ++  E+ R++R T +D T    ++S     +  K+++ S ++     + +R+   +S  M+QEEI+
Sbjct:  262 PYFNTLENSIVNLFVLLTTANFPDVMMPSYSRNPWSCVFFIVYLSIELYFIMNLLLAVVFDTFNDIEKHKFKSLLLHKRTAIQHAYHLL-----VSQRRPAG-------ISYRQFEGLM-RFYKPRMSARERFLTFKALNQSNTPLLSLKDFYDIYEVAALQWKAKKNRQHWFDELPRTAFLIFKGINILVNSKAFQYFMYLVVAVNGVWILVETFMLKGGNFISKHVPWSYLVFLTIYGVELFMKVAGLGPVEYLSSGWNLFDFSVTAFAFLGLLA--LTLNMEPFYFIVVLRPLQLLRLFKLK-KRYRNVLDTMFELLPRMASLGLTLLTFYYSFAIVGMEFFSGRLSPNCCNSSTVADAYRFINHTVGNKTKVE-----EGYYYLNNFDNILNSFVTLFELTVVNNWYIIMEG--VTSQTSHWSRLYFMTFYIVTMVVMTIIVAFILEAFVFRMNYSRKSQESEVDSGIVIEKEMS--KEELLAILELHREARGTSSDVTRLLDTLS-----QMEKYQQNSMVFL---GRRSRTKSDLSLKMYQEEIQ 779
[Close]
BLAST of two pore calcium channel protein 1 vs. UniProt/SwissProt
Match: sp|Q9ULQ1|TPC1_HUMAN (Two pore calcium channel protein 1 OS=Homo sapiens OX=9606 GN=TPCN1 PE=1 SV=3)

HSP 1 Score: 88.9669 bits (219), Expect = 7.248e-17
Identity = 125/558 (22.40%), Postives = 258/558 (46.24%), Query Frame = 1 
Query:  706 PYMQNFFDIVWETLIFITSSNSPDVILPGYSKSSYIGILFLIFYAM-FYFLLIFVYGLIMGNFRYHTKEEMTKLLVGKISLMYDAFVLLAEKIVINEKKNCNELFEKNIIKKETWKNLIARFKKYQNCEEAEEIMYSLLSQTNKTYLMKNEFLQMADLMSIPLTIVTNKFHFFE---KNFWFVFSNKLADFVESKYFNLIVGIVIVINSCCI---TFTSE---------PYXXXXXXXXXXXXXXXRIYVKGFIRYFHSLWNSFDFVVIVLAFIFWFVENMKLNVQTITFTXXXXXXXXXXXXXXGIAAYHQLLHILGMLIPTISSL-ITIV-------------FCFIVFYVCIGIWSYQDMFLMNRSELDYMFNSSSCNSTKLEGSDFGRQHYCILSFNSFISGFLTLCSVSIGNNWHTITEGYELESESMILSRFYFSVFKGVLTYLLLLIVAFVLDVFLLAYN---QKSSLKIEELIKNKLNSLKINFISYGEI-RKSRLTKTDSTEGSQSISLTRYFKRVKFEETSRIWFRLKAKGARSVEYVSQAMFQEEIE 2277
            PY     + +    + +T++N PDV++P YS++ +  + F+++ ++  YF++  +  ++   F    K +   LL+ K + +  A+ LL     I++++          I    ++ L+ RF K +       + +  L+Q N   L   +F  + ++ ++      N+ H+F+   +    +F   +   V+SK F   + +V+ +N   I   TF  +         P++ L F  ++ +E+ L++   G + Y  S WN FDF V V AF+   +  + LN++   F +++     L         Y  +L  +  L+P ++SL +T++             FC IVF  C    +  D +           N +  N T +E       +Y + +F++ ++ F+TL  +++ NNW+ I EG  + S++   SR YF  F  V   ++ +IVAF+L+ F+   N   +    +++  I  +    K   ++  E+ R++R   +D T   +++S     +  ++++ S ++     + +R+   +S  M+QEEI+
Sbjct:  261 PYFSTLENSIVSLFVLLTTANFPDVMMPSYSRNPWSCVFFIVYLSIELYFIMNLLLAVVFDTFNDIEKRKFKSLLLHKRTAIQHAYRLL-----ISQRRPAG-------ISYRQFEGLM-RFYKPRMSARERYLTFKALNQNNTPLLSLKDFYDIYEVAALKWKAKKNREHWFDELPRTALLIFKG-INILVKSKAFQYFMYLVVAVNGVWILVETFMLKGGNFFSKHVPWSYLVFLTIYGVELFLKVAGLGPVEYLSSGWNLFDFSVTVFAFL--GLLALALNMEPFYFIVVLRPLQLLRLFKLK-ERYRNVLDTMFELLPRMASLGLTLLIFYYSFAIVGMEFFCGIVFPNCCNTSTVADAYRWR--------NHTVGNRTVVEEG-----YYYLNNFDNILNSFVTLFELTVVNNWYIIMEG--VTSQTSHWSRLYFMTFYIVTMVVMTIIVAFILEAFVFRMNYSRKNQDSEVDGGITLEKEISKEELVAVLELYREARGASSDVTRLLETLS-----QMERYQQHSMVFL---GRRSRTKSDLSLKMYQEEIQ 778
[Close]
BLAST of two pore calcium channel protein 1 vs. UniProt/SwissProt
Match: sp|Q94KI8|TPC1_ARATH (Two pore calcium channel protein 1 OS=Arabidopsis thaliana OX=3702 GN=TPC1 PE=1 SV=1)

HSP 1 Score: 79.337 bits (194), Expect = 7.880e-14
Identity = 116/526 (22.05%), Postives = 233/526 (44.30%), Query Frame = 1 
Query:  493 FRISPILRVLLFINSAEQSQXXXXXXXXXXXXXNVLVILPFLITFYFMMVAFIYRYFKINKSEPVFYYNKRPYMQNFFDIVWETLIFITSSNSPDVILPGYSKSSYIGILFLIFYAM-FYFLLIFVYGLIMGNFRYHTKEEMTKLLVGKISLMYDAFVLL-AEKIVINEKKNCNELFEKNIIKKETWKNLIARFKKYQNCEEAE-EIMYSLLSQTNKTYLMKNEFLQMADLMSIPLTI--VTNKFHFFEKNFWFVFSNKLADFVESKYFNLIVGIVIVINSCCITFTS----------EPYXXXXXX--XXXXXXXXXRIYVKGFIRYFHSLWNSFDFVV---IVLAFIFWFV---ENMKLNVQTITFTXXXXXXXXXXXXXXGIAAYHQLLHILGMLIPTISSLITIVFCFIVFYVCIGIWSYQDMFLMNRSELDYMFNSSSCNSTKLEGSDFGRQHYCILSFNSFISGFLTLCSVSIGNNWHTITEGYELESESMILSRFYFSVFKGVLTYLLLLIVAFVLDVF 2001
            FRI+P +RV++FI S  + +    L +  +     ++ L  L   +   +AF+  +    +   VF         ++   +++  I  T+SN+PDV +P Y  S +  + F+++  +  YF+   +  ++  +F+    ++++ +   K  ++  AF L+ ++K    +K  C +LFE+           +  ++      + E  +++  L  T    + K+EF  +   +++      V + F  F + +    S +L  FV S  F   +  +++IN   +   +          +P+    F    +++LE+ L+IY  GF  Y+    N FDF+V   IV+     F+   EN   +       LL+ R LRL++LL  +  Y   +     LIP++   +  +FC +  Y  IG+  +  +               +  + KL  ++     Y + +FN + +G +TL ++ +  NW    E Y+  + +     ++ S +   +  LL L+VAFVL+ F
Sbjct:  184 FRIAPYVRVIIFILSIRELRDTLVLLSGMLGTYLNILALWMLFLLFASWIAFVM-FEDTQQGLTVF--------TSYGATLYQMFILFTTSNNPDVWIPAYKSSRWSSVFFVLYVLIGVYFVTNLILAVVYDSFKEQLAKQVSGMDQMKRRMLEKAFGLIDSDKNGEIDKNQCIKLFEQ-----------LTNYRTLPKISKEEFGLIFDELDDTRDFKINKDEFADLCQAIALRFQKEEVPSLFEHFPQIYHSALSQQLRAFVRSPNFGYAISFILIINFIAVVVETTLDIEESSAQKPWQVAEFVFGWIYVLEMALKIYTYGFENYWREGANRFDFLVTWVIVIGETATFITPDENTFFSNGEWIRYLLLARMLRLIRLLMNVQRYRAFIATFITLIPSLMPYLGTIFCVLCIYCSIGVQVFGGLV--------------NAGNKKLFETELAEDDYLLFNFNDYPNGMVTLFNLLVMGNWQVWMESYKDLTGTWWSITYFVSFYVITILLLLNLVVAFVLEAF 675
[Close]
BLAST of two pore calcium channel protein 1 vs. UniProt/SwissProt
Match: sp|Q75VR1|TPC1A_TOBAC (Two pore calcium channel protein 1A OS=Nicotiana tabacum OX=4097 GN=TPC1A PE=2 SV=1)

HSP 1 Score: 73.559 bits (179), Expect = 3.992e-12
Identity = 138/579 (23.83%), Postives = 244/579 (42.14%), Query Frame = 1 
Query:  493 FRISPILRVLLFI-NSAEQSQXXXXXXXXXXXXXNVLVILPFLITFYFMMVAFIYRYFKINKSEPVFYYNKRPYMQNFFDIVWETLIFITSSNSPDVILPGYSKSSYIGILFLIFYAMFYFLLI-FVYGLIMGNFRYHTKEEMTKLLVGKISL----MYDAFVLL--AEKIVINEKKNCNELFEKNIIKKETWKNLIARFKKYQNCEE-AEEIMYSLLSQTNKTYLMKNEFLQMADL-MSIPLTIV-TNKFHFFE--KNFWF-VFSNKLADFVESKYFNLIVGIVIVINSCCITFTSE------------PYXXXXXXXXXXXXXXXRIYVKGFIRYFHSLWNSFDFVV---IVLAFIFWFVENMKL----NVQTITFTXXXXXXXXXXXXXXGIAAYHQLLHILGMLIPTISSLITIVFCFIVFYVCIGIWSYQDMFLMNRSELDYMFNSSSCNSTKLEGSDFGRQHYCILSFNSFISGFLTLCSVSIGNNWHTITEGYELESESMILSRFYFSVFKGVLTYLLLLIVAFVLDVFLLAYNQKSSLKIEELIKNKLNSLKINFISYGEIRKSRLTKTDS 2130
            FRI+P LRV+ FI N  E       L  +  T +NV+ +    + F   +    Y +F+  +         +    ++   +++  +  T+SN+PDV +P Y  S +    + +F+ ++  L + FV  LI+       K E+ K +  K  L    +  AF L+  A    +NEK+ C  LFE+                KY+   + + +   S+ S+ + T   K    + ADL  +I L     +    FE   NF+    S KL  FV    F  I+  V+++N   +   +                E  F  L+++E+ L++Y  GF  Y+    N FDFVV   IV+     FV    L    N + I + L+      +  L+  +  Y   +     LIP++   +  +FC + FY  +G+  +  +            N+ + N   L  +D     Y + +FN + +G +TL ++ +  NW    + Y+  + +     ++ S +   + +LL LIVAFVL+ F    + ++S +  +    +  S         E R++  TKT S
Sbjct:  187 FRIAPYLRVVFFILNIRELRDSFFILAGMLGTYLNVVALSALFLLFSSWLA---YVFFEDTRQ-------GKTTFTSYGTTLYQMFVLFTTSNNPDVWIPAYKDSRW----YCLFFVLYVLLGVYFVTNLILAVVYDSFKSELVKQVADKDRLRLRTLKKAFSLIDEANNGHLNEKQ-CTLLFEE--------------LNKYRTLPKISGDDFKSIFSELDDTGDFKINLDEFADLCTAIGLRFQKEDSLPIFEACPNFYHSPASEKLRGFVRGATFEYIIVFVLLVNLVAVIIETTLDIQNNSGQTFWQKVEFTFGWLYVIEMALKVYTYGFENYWRDGQNRFDFVVTWVIVIGETATFVAPDGLTFLSNGEWIRYLLIARMLRLIRLLMH-VERYRAFVATFFTLIPSLVPYLGTIFCILCFYCSLGLQIFGGIV-----------NTGNPN---LAQTDLAGNDYLLFNFNDYPNGMVTLFNILVMGNWQVWMQSYKELTGTAWTYAYFVSFYLISVLWLLNLIVAFVLEAFQAEVDLEASARCVDGDDKEAKS---------ERRRNVGTKTRS 712
[Close]
BLAST of two pore calcium channel protein 1 vs. TrEMBL
Match: A0A2C9KPG9 (Uncharacterized protein OS=Biomphalaria glabrata OX=6526 GN=106057223 PE=4 SV=1)

HSP 1 Score: 174.866 bits (442), Expect = 1.030e-41
Identity = 194/766 (25.33%), Postives = 353/766 (46.08%), Query Frame = 1 
Query:  100 SFSQDTLDLAFQLISDASVGRLTNFERTVTCLKYYRIYLSRTFRFIRLIIHLTLVI---LLMYDASNKDSLSLIAKITIPIECACLSFYAIQLFIVYKFSIKSQFIKDKKHYCXXXXXXXXXXXXXXF------KSRVYQFRISPILRVLLFINSAEQSQXXXXXXXXXXXXXNVLVILPFLITFYFMMVAFIYRYFKINKSEPVFYYNKRPYMQNFFDIVWETLIFITSSNSPDVILPGYSKSSYIGILFLIFYAM-FYFLLIFVYGLIMGNFRYHTKEEMTKLLVGKISLMYDAFVLLAEKIVINEKKNCNELFEKNIIKKETWKNLIARFKKYQNCEEAEEIMYSLLSQTNKTYLMKNEFLQMADLMSIPLTIVTNKFHFFEKNFWFVFSNKLAD----FVESKYFNLIVGIVIVINSCCITFTSEPYXXXXXXXXXXXXXXXRIYVKGFIRYFHSLWNSFDFVVIVLAFIFWFVE-----NMKLNVQTITFTXXXXXXXXXXXXXXGIAAYHQLLHILGMLIPTISSLITIVFCFIVFYVCIGIWSYQDMFLMNRSELDYMFNSSS-----CNSTKLEGSDFGRQHYCILSFNSFISGFLTLCSVSIGNNWHTITEGYELESESMILSRFYFSVFK-GVLTYLLLLIVAFVLDVFLLAYNQKSSLKIEELIKNKLNSL-----------KINFISYGEIRKSRLTKTDSTE--GSQSISLTRYFKRVKFEETSRIWFRLKAKGARSVEYVSQAMFQEEIEAD 2283
               ++TL LA  LI DA  GR  N E  +   KY R Y+     ++R  ++  L     L +++       S+    T+ +E  CL ++  +L   + F     F KD K+ C  + IL++++D+I +       + V   R S  LR L  IN  +  QIRR  R +R TI +++ +L   ++   +   F  + F         Y N  PY  N+FD +W+  + +T++N+PDV++P Y +S++  + F+I+  +  Y  +  V   I  N++ + K E+ + +  K   +  AF LL        K N  E      +    W  L+      ++ E   +++  +L   N   L K  FL +ADL+ + LT V ++    EK+F  ++++++++    FV  + F     ++I++N+  I    +   + FF  +F +EI+ ++YV G   +    WN FD V+I  AF+   +E     N + N++ I   L V+R ++L      I  +  +L  L  + P+  +   ++F F  F+  +G      M + N     Y ++S +     C + KL  S F R+HYC  +FN  +   + +  +++ N WH I  G+   +  +  +R YF VF    +  +L +  AF+L+ F+L Y  ++  ++E ++++K+  L            I   S  EI      +T+ T+   +Q +        +   +   + F LK    +  E + Q MFQ E+  D
Sbjct:   38 PLDKETLLLAGTLILDAKGGR--NLEYILRE-KYVRSYIWYNSFYLRWALYFCLAFNLSLALFEHPAVPGASIPYWGTMIMELVCLCYFTFRLSHAFYFQYTKVFFKDAKNICVLVMILLTILDMISYIIWSNVDTDVPPVRWSRPLRPLFIINFPDGKQIRRAFRNIRRTIPDIVNVLILFLSSVLLFGLFALKLFN---KRGFKYPNGEPYFSNYFDSIWDLYVLVTTANNPDVMMPAYDESNWFALFFVIYLILCLYIFMSIVLATIYNNYKKNLKNEVKEAVFQKRKKLAKAFDLL------KVKHNHQEF-----VSYSRWTQLVTIILPGRS-ETHTDLLMKILDHDNTNVLSKKNFLNLADLLQVQLTEVKDRLTLLEKHFPSIYNHRISEYFKFFVRHRVFRYWFDLLIIVNAVFIAIDLDA-ADWFFLAMFTVEILSKLYVLGGKEFMSRFWNIFDVVIIGGAFVATIIEYLDFDNKESNIKDILLVLRVVRLIKLFGT---IKRFKVILQTLINIGPSFFTYGGVIFVFYYFFAIVG------MEIFNGYITYYGYDSETQDTAFCGNVKLNNSIFYREHYCNNNFNDILKSMVVMFELTVVNQWHVIASGFVAVTSKV--ARLYFFVFHLSCVVIILNIFTAFILEAFILEYTLQTVPRLESVVESKIKELGLGIGMKTRKHAIQGPSGDEIILVEDEQTEDTDEVDNQQVETEDVDNEIDLSKEKGLKFHLKKHSRKKTEVLLQQMFQGELGDD 773
[Close]
BLAST of two pore calcium channel protein 1 vs. TrEMBL
Match: A0A267F8Q1 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig007485g3 PE=4 SV=1)

HSP 1 Score: 169.859 bits (429), Expect = 4.535e-40
Identity = 201/745 (26.98%), Postives = 358/745 (48.05%), Query Frame = 1 
Query:  124 LAFQLISDASVGRLTNFERTVTCLKYYRIYL--SRTFRFIRLIIHLTLVILLMYDASNKDSLSLIAKIT--IP--IECACLSFYAIQLFIVYKFSIKSQFIKDKKHYCXXXXXXXXXXXXXXF----------KSRVYQFRISPILRVLLFINSAEQSQXXXX---XXXXXXXXXNVLVILPFLITFYFMMVAFIYRYFKINKSEPVFYYNKRPYMQNFFDIVWETLIFITSSNSPDVILPGYSKSSY--IGILFLIFYAMFYFLLIFVYGLIMGNFRYHTKEEMTKLLVGKISLMYDAFVLLAEKIVINEKKNCNELFEKNIIKKETWKNLIARFKKYQNCEEAEEIMYSLLSQTNKTYLMKNEFLQMADLMSIPLTIVTNKFHFFEKNFWFVFSNKLADF----VESKYFNLIVGIVIVINSCCITFTSEPYXXXXXXXXXXXXXXXRIYVKGFIRYFHSLWNSFDFVVIVLAFIFWFVENM--KLNVQTITFTXXXXXXXXXXXXXXG-IAAYHQLLHILGMLIPTISSLITIVFCFIVFYVCIGIWSYQDMFLMNRSELDYMFNSSSCNSTKLEGSDFGRQHYCILSFNSFISGFLTLCSVSIGNNWHTITEGYELESESMILSRFYFSVFKGVLTYLLLLI-VAFVLDVFLLAYNQKSSLKIEELIKNKLNSLKINFISYGEIRKSRLTKTDSTE----GSQSISLTRYFKRVKFEETSRIWFRLKAKGARSVEYVSQAM 2259
            LA  L++D+ VGR   F+     L+ Y++Y   + T  F   I+   LVI++      +  +  +A++   IP  IEC+C+ F+A +++  +  +   QF +D K+    + I +++ID+  F           + V  FRI   LR L  IN  E  QIRR     R   + I  V +++ FL+  + ++     + F    S  + Y N  PY +++ + +W+  I IT++NSPDV++P Y  +++  I     +    + F+ IF+  +I  N++ + K E+   +  K  L+  AF  L     IN   +  +     ++ K+ W  L       +N E A+  +  L   TN  YL K +F ++ DL+++ +T + ++    E     V+++  +      V  ++F     ++IV+N+  I F+   +TE +F   F+LEI+L++YV G + +F   WN FDFVVI  A I  F E +  +  ++  T  ++++  +  L  + G I  +  +L  +  +   +S    IV+     Y  +G+  +       R+E+    +   C +  L  S+F R  YC  +FN  +  ++TL  ++I N WH ITEG+ L +     SR YF  F  +   L++ I +AF+L+ FLL YN  +S K E  I+ K+  +      +  + ++ L + D+ E    GSQ+               S+I F +K +  +SVE + Q M
Sbjct:   65 LAAILLTDSVVGRSMPFKADPKSLRSYQLYCHWAMTIIFYVFIVADLLVIVM-----ERPQIPAMAEVPVWIPLLIECSCIGFFAFRIWWYFSITETVQFFRDIKNIVVVVCIALNVIDVFCFLIWFGVSGSADAPVRWFRI---LRPLYMINFPEGKQIRRAFRNTRKTLVKIATVFILVGFLMAVFALLA---MKLFSARASRTLRYPNGAPYFESYLESIWDLYILITTANSPDVMMPSYDANNFYIIFFFVFVVVCNYIFMSIFL-AVIYNNYKNYLKSEVRTSVHIKRRLIGRAFDCLK----INRVPDLGD-----VVTKDAWFRLFKTVYPNKNSELAQLFLNVLNGGTND-YLTKAQFTRLPDLLNVGVTELKDRTLLLESCLPTVYNSAPSQLIKFIVRHRFFKYTFDLLIVVNAFLIAFSVN-FTEWYFLGAFVLEILLKLYVMGPVAFFRRAWNIFDFVVIFSALIITFFEEVSNERRLEEGTLDIILVLRVVRLLRIFGRIQRFRVILTTIANIRSPLSVYAAIVWVLYYMYGVLGMELFSGRI---RAEVYTAQSPKFCYNNALNNSEFYRMRYCSNNFNDPLKAWVTLFELTIVNQWHVITEGFVLVTNK--FSRLYFMSFHLICVILVINIFIAFILEAFLLEYNLSAS-KFESDIEQKIKEM-----GFSAVSEAILDEEDAEEQFELGSQA--------------ASQIRFHIKKRARKSVETLLQQM 761
[Close]
BLAST of two pore calcium channel protein 1 vs. TrEMBL
Match: A0A1I8J6U5 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 169.859 bits (429), Expect = 5.402e-40
Identity = 201/745 (26.98%), Postives = 358/745 (48.05%), Query Frame = 1 
Query:  124 LAFQLISDASVGRLTNFERTVTCLKYYRIYL--SRTFRFIRLIIHLTLVILLMYDASNKDSLSLIAKIT--IP--IECACLSFYAIQLFIVYKFSIKSQFIKDKKHYCXXXXXXXXXXXXXXF----------KSRVYQFRISPILRVLLFINSAEQSQXXXX---XXXXXXXXXNVLVILPFLITFYFMMVAFIYRYFKINKSEPVFYYNKRPYMQNFFDIVWETLIFITSSNSPDVILPGYSKSSY--IGILFLIFYAMFYFLLIFVYGLIMGNFRYHTKEEMTKLLVGKISLMYDAFVLLAEKIVINEKKNCNELFEKNIIKKETWKNLIARFKKYQNCEEAEEIMYSLLSQTNKTYLMKNEFLQMADLMSIPLTIVTNKFHFFEKNFWFVFSNKLADF----VESKYFNLIVGIVIVINSCCITFTSEPYXXXXXXXXXXXXXXXRIYVKGFIRYFHSLWNSFDFVVIVLAFIFWFVENM--KLNVQTITFTXXXXXXXXXXXXXXG-IAAYHQLLHILGMLIPTISSLITIVFCFIVFYVCIGIWSYQDMFLMNRSELDYMFNSSSCNSTKLEGSDFGRQHYCILSFNSFISGFLTLCSVSIGNNWHTITEGYELESESMILSRFYFSVFKGVLTYLLLLI-VAFVLDVFLLAYNQKSSLKIEELIKNKLNSLKINFISYGEIRKSRLTKTDSTE----GSQSISLTRYFKRVKFEETSRIWFRLKAKGARSVEYVSQAM 2259
            LA  L++D+ VGR   F+     L+ Y++Y   + T  F   I+   LVI++      +  +  +A++   IP  IEC+C+ F+A +++  +  +   QF +D K+    + I +++ID+  F           + V  FRI   LR L  IN  E  QIRR     R   + I  V +++ FL+  + ++     + F    S  + Y N  PY +++ + +W+  I IT++NSPDV++P Y  +++  I     +    + F+ IF+  +I  N++ + K E+   +  K  L+  AF  L     IN   +  +     ++ K+ W  L       +N E A+  +  L   TN  YL K +F ++ DL+++ +T + ++    E     V+++  +      V  ++F     ++IV+N+  I F+   +TE +F   F+LEI+L++YV G + +F   WN FDFVVI  A I  F E +  +  ++  T  ++++  +  L  + G I  +  +L  +  +   +S    IV+     Y  +G+  +       R+E+    +   C +  L  S+F R  YC  +FN  +  ++TL  ++I N WH ITEG+ L +     SR YF  F  +   L++ I +AF+L+ FLL YN  +S K E  I+ K+  +      +  + ++ L + D+ E    GSQ+               S+I F +K +  +SVE + Q M
Sbjct:   70 LAAILLTDSVVGRSMPFKADPKSLRSYQLYCHWAMTIIFYVFIVADLLVIVM-----ERPQIPAMAEVPVWIPLLIECSCIGFFAFRIWWYFSITETVQFFRDIKNIVVVVCIALNVIDVFCFLIWFGVSGSADAPVRWFRI---LRPLYMINFPEGKQIRRAFRNTRKTLVKIATVFILVGFLMAVFALLA---MKLFSARASRTLRYPNGAPYFESYLESIWDLYILITTANSPDVMMPSYDANNFYIIFFFVFVVVCNYIFMSIFL-AVIYNNYKNYLKSEVRTSVHIKRRLIGRAFDCLK----INRVPDLGD-----VVTKDAWFRLFKTVYPNKNSELAQLFLNVLNGGTND-YLTKAQFTRLPDLLNVGVTELKDRTLLLESCLPTVYNSAPSQLIKFIVRHRFFKYTFDLLIVVNAFLIAFSVN-FTEWYFLGAFVLEILLKLYVMGPVAFFRRAWNIFDFVVIFSALIITFFEEVSNERRLEEGTLDIILVLRVVRLLRIFGRIQRFRVILTTIANIRSPLSVYAAIVWVLYYMYGVLGMELFSGRI---RAEVYTAQSPKFCYNNALNNSEFYRMRYCSNNFNDPLKAWVTLFELTIVNQWHVITEGFVLVTNK--FSRLYFMSFHLICVILVINIFIAFILEAFLLEYNLSAS-KFESDIEQKIKEM-----GFSAVSEAILDEEDAEEQFELGSQA--------------ASQIRFHIKKRARKSVETLLQQM 766
[Close]
BLAST of two pore calcium channel protein 1 vs. TrEMBL
Match: A0A2B4SLZ3 (Two pore calcium channel protein 1 OS=Stylophora pistillata OX=50429 GN=Tpcn1 PE=4 SV=1)

HSP 1 Score: 166.392 bits (420), Expect = 6.133e-39
Identity = 187/759 (24.64%), Postives = 370/759 (48.75%), Query Frame = 1 
Query:  100 SFSQDTLDLAFQLISDASVGRLTNFERTVTCLKYYRIYLSRTFRFIRLIIHLTLVILLMYDASNKDSLSLIAKITIPIECACLSFYAIQLFIVYKFSIKSQFIKDKKHYCXXXXXXXXXXXXXXF------KSRVYQFRISPILRVLLFINSAEQS---QXXXXXXXXXXXXXNVLVILPFLITFYFMMVAFIYRYFKINKSEPVFYYNKRPYMQNFFDIVWETLIFITSSNSPDVILPGYSKSSYIGILFLIF--YAMFYFLLIFVYGLIMGNFRYHTKEEMTKLLVGKISLMYDAFVLLAEKIVINEKKNCNELFEKNIIKKETWKNLIARFKKYQNCEEAEEIMYSLLSQTNKTYLMKNEFLQMADLMSIPLTIVTNKFHFFEKNFWFVFSNKLADFVE----SKYFNLIVGIVIVINSCCITFTSEPYXXXXXXXXXXXXXXXRIYVKGFIRYFHSLWNSFDFVVIVLAFIFWFVENMKLNVQTITFTXXXXXXXXXXXXXXGIAAYHQLLHILGMLI---PTISSLITIVFCFIVFYVCIGIWSYQDMFLMNRSELDYMFNSSS------------CNSTKLEGSDFGRQHYCILSFNSFISGFLTLCSVSIGNNWHTITEGYELESESMILSRFYFSVFKGVLTYLLLLI-VAFVLDVFLLAYNQKSSLKIEELIKNKLNSLKINFISYGEIRKSRLTKTDSTEGSQSISLTRYFKRVKFEETSRIWFRLKAKGARSVEYVSQAMFQEEIEAD 2283
              ++  L LA  L+++A +GR  +F+ +   L+ Y+IY     + +        + L  ++      ++L    T+ +E  CL  + ++LF V+ F+   +F +DKK+      I+++ +D+I++      K  V+  R + +LR L+ IN +E     +  R++R     I NVLV+L  +I  + ++ + ++   K N  +     + +PY  N+FD+ ++  +  T++N+PD+++P Y  +++  + F+IF    M+ F+ IF+  ++  N+R H K E+   +  K   + +AF +L        K   NE++   ++ ++ W  L+       +      +++ +L + N+  + + +FL +ADL+++ +  V ++ + FE+    V++++++  +      KYF     +VI  N+  I        E+ F  LF +EI L++Y  GF  +F   WN FDF+VI  A +   +E    ++Q+    L  L  LR+L+L++ +    +   I+G ++   P +S+   I+F     Y  +G+  + ++    +SE  Y                  C + KL+GSDF    YC  +FN+ +  F  L  + + N WH IT+GY L +     +R YF  F  +   ++L I VAF+L+ F+L Y+     KIE +I  K+    +  +    +R+   +     E ++S S   +         + + FR+  +  ++ + + Q MF++E+  D
Sbjct:   45 ELNETELLLAGILVNEARLGRNGHFKTSPKELRLYKIYNKPVLQILVYFCVWLNLCLAFFEEPAVQGMALPYWATMLLEYICLFVFILRLFHVWCFAAGVKFWRDKKNVIMLSIIVLTFLDMIMYVIFKEAKIPVHTTRWTRVLRPLILINISEGRHIRRAVRNIRKTLPEIANVLVLLSLMIALFTLLGSKLFG--KRNLKD----LHGKPYFMNYFDVYFKLYVLTTTANNPDIMMPAYDNNAWSSLFFVIFIVVCMYIFVSIFL-AVVYKNYRMHMKNEIRASVYRKRRQLKNAFDVL--------KVWSNEMW---VVTQKRWLTLMKEVCPQWSLARVA-LLWQVLDENNEGKIGRKDFLMLADLLNVKVIEVKDQVNLFERFIPRVYNSRISAKIRLGVCHKYFVYFFDLVIFANAIFIALERND-LEVLFLALFNVEIFLKLYTYGFKEFFARYWNIFDFLVIGAATLALIIETSYESLQSSQGVLDFLMVLRVLRLVKIMGQIKRFQVIIGTVMQVGPAMSTYGAIMFVLYYIYAIMGMELFGNLI---KSESSYPTPGGGSSDGENLTSTMFCGNIKLKGSDFYADRYCNNNFNNILRSFKVLFDLMVVNQWHIITQGYVLVTNK--FARLYFLSFHLLCVIVVLNIFVAFILEAFMLEYSFTHG-KIETVINKKIEEKGVG-VGMCPVRERAASLLSIDEVTESYSNGGFSSLEMLAMDTGLRFRMPKQQRKNADVLLQEMFEDELGED 776
[Close]
BLAST of two pore calcium channel protein 1 vs. TrEMBL
Match: A0A1I8G189 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 166.007 bits (419), Expect = 8.216e-39
Identity = 198/745 (26.58%), Postives = 358/745 (48.05%), Query Frame = 1 
Query:  124 LAFQLISDASVGRLTNFERTVTCLKYYRIYL--SRTFRFIRLIIHLTLVILLMYDASNKDSLSLIAKIT--IPI--ECACLSFYAIQLFIVYKFSIKSQFIKDKKHYCXXXXXXXXXXXXXXF----------KSRVYQFRISPILRVLLFINSAEQSQXXXX---XXXXXXXXXNVLVILPFLITFYFMMVAFIYRYFKINKSEPVFYYNKRPYMQNFFDIVWETLIFITSSNSPDVILPGYSKSSY--IGILFLIFYAMFYFLLIFVYGLIMGNFRYHTKEEMTKLLVGKISLMYDAFVLLAEKIVINEKKNCNELFEKNIIKKETWKNLIARFKKYQNCEEAEEIMYSLLSQTNKTYLMKNEFLQMADLMSIPLTIVTNKFHFFEKNFWFVFSNKLADF----VESKYFNLIVGIVIVINSCCITFTSEPYXXXXXXXXXXXXXXXRIYVKGFIRYFHSLWNSFDFVVIVLAFIFWFVENM--KLNVQTITFTXXXXXXXXXXXXXXG-IAAYHQLLHILGMLIPTISSLITIVFCFIVFYVCIGIWSYQDMFLMNRSELDYMFNSSSCNSTKLEGSDFGRQHYCILSFNSFISGFLTLCSVSIGNNWHTITEGYELESESMILSRFYFSVFKGVLTYLLLLI-VAFVLDVFLLAYNQKSSLKIEELIKNKLNSLKINFISYGEIRKSRLTKTDSTE----GSQSISLTRYFKRVKFEETSRIWFRLKAKGARSVEYVSQAM 2259
            LA  L++D+ VGR   F+     L+ Y++Y   + T  F   I+   LVI++      +  +  +A++   IP+  EC+C+ F+A +++  +  +   QF +D K+    + I +++ID+  F           + V  FRI   LR L  IN  E  QIRR     R   + I  V +++ FL+  + ++     + F    S  + Y N  PY +++ + +W+  I IT++NSPDV++P Y  +++  I     +    + F+ IF+  +I  N++ + K E+   +  K  L+  AF  L     IN   +  +     ++ K+ W  L       ++ E A+  +  L   TN  YL K +F ++ DL+++ +T + ++    E     V+++  +      V  ++F     ++IV+N+  I F+   +TE +F   F+LEI+L++YV G + +F   WN FDFVVI  A +  F E +  +  ++  T  ++++  +  L  + G I  +  +L  +  +   +S    IV+     Y  +G+  +       R+E+    +   C +  L  S+F R  YC  +FN  +  ++TL  ++I N WH ITEG+ L +     SR YF  F  +   L++ I +AF+L+ FLL YN  +S K E  I+ K+  +      +  + ++ L + D+ E    GSQ+               S+I F +K +  +SVE + Q M
Sbjct:   70 LAAILLTDSVVGRSMPFKSDPKSLRSYQLYCHWAMTIIFYVFIVADLLVIVM-----ERPQIPAMAEVPVWIPLLLECSCIGFFAFRIWWYFSITESVQFFRDIKNIVVVVCIALNVIDVFCFLIWFGVSGSADAPVRWFRI---LRPLYMINFPEGKQIRRAFRNTRKTLVKIATVFILVGFLMAVFALLA---MKLFSARASRTLRYPNGAPYFESYLESIWDLYILITTANSPDVMMPSYDANNFYIIFFFVFVVVCNYIFMSIFL-AVIYNNYKNYLKSEVRTSVHIKRRLIGRAFDCLK----INSLPDLGD-----VVTKDAWFRLFKTIYPNKSSELAQLFLNVLNGGTND-YLTKAQFTRLPDLLNVGVTELKDRTLLLESCLPTVYNSAPSQLIKFIVRHRFFKYTFDLLIVVNAFLIAFSVN-FTEWYFLGAFVLEILLKLYVMGPVAFFRRAWNIFDFVVIFSALMITFFEEVSNERRLEEGTLDIILVLRVVRLLRIFGRIQRFRVILTTIANIRSPLSVYAAIVWVLYYMYGVLGMELFSGRI---RAEVYTAQSPKFCYNNALNNSEFYRMRYCSNNFNDPLKAWVTLFELTIVNQWHVITEGFVLVTNK--FSRLYFMSFHLICVILVINIFIAFILEAFLLEYNLSAS-KFESDIEQKIKEM-----GFSAVSEAILDEEDAEEQFELGSQA--------------ASQIRFHIKKRARKSVETLLQQM 766
[Close]
BLAST of two pore calcium channel protein 1 vs. Ensembl Cavefish
Match: tpcn3 (two pore segment channel 3 [Source:ZFIN;Acc:ZDB-GENE-141215-16])

HSP 1 Score: 112.464 bits (280), Expect = 4.569e-25
Identity = 158/620 (25.48%), Postives = 283/620 (45.65%), Query Frame = 1 
Query:  100 SFSQDTLDLAFQLISDASVGRLTNFERTVTCLKYYRIYLSRTFRFIRLIIHLTLVILLMYDASNKDSLSLIAKITIPIECACLSFYAIQLFIVY--KFSIKSQFIKDKKHYCXXXXXXX----XXXXXXXFKSRVYQFRISPILRVLLFINSAEQSQXXXXXXXXXXXXXNVLVILPFLITFYFMMVAFIYRYFKINKSEPVFYYNKRPYMQNFFDIVWETLIFITSSNSPDVILPGYSKSSYIGILFLIFYAM--FYFLLIFVYGLIMGNFRYHTKEEMTKLLVGKISLMYDAFVLLAEKIVINEKKNCNELFEKNIIKKETWKNLIARFKKYQNCEEAE-EIMYSLLSQTNKTYLMKNEFLQMADLMSIPLTIVTNKFHFFEKNFWF------VFSNKLADFVESKYFNLIVGIVIVINSCCITFTSEPYXXXXXXXXXXXXXXXRIYVKGFI---RYF---HSLWNSFDFVVIVLAFIFWFVENMKL---------NVQTITFTXXXXXXXXXXXXXXGIAAYHQLLHILGMLIPTISSLITIVFCFIVFYVCIGIWSYQ-DMFLMNRSELDYMFNSSSCNSTKLEGSDFGRQHYCILSFNSFISGFLTLCSVSIGN 1866
            S SQ   D   Q+    S  R   F+ +   ++ Y +Y       +  +  +  + L +++     SL L A  T  +E  CL   AI L +++  K   +  F +D K+ C  + I +     +I   L+ S     R S +LR LL +N  E  Q+RR  R++R  +  +LV+      F F ++ F     K+     +   +   Y  N+ + V+E  + +T++NSPDV++P Y+ SSY  I F+++  +  + F+ +F+  ++  N++ H KEE+ +L+  K   +  AF LL E I  N            ++ +  W  L+ + +   N   A  E+++S+L   NK ++ K  FLQ+ADL++I +  + ++ H F+   W         S  +   V+ + F  +   VI++N+  I    E          FL   +L I +K +    R F   H  WN FD ++I+ A +   + N  L          +  I F L VLR +R++  +    A    L  +G  + T   LI +V+ +I   V + ++  +  +F  N ++    +    C +  L+ S F R +YC  +FN  +S F+ L  +++ N
Sbjct:   32 SQSQQGRDQTGQV--SHSYNRNIPFDPSPQAVRLYLLYNHGLLHVLVYVFIMVDLALALFEEPAVVSLPLWA--TSAMELVCL--LAITLRVIHYSKVIPRRIFFRDPKNLCIIVIITLFLIDMIIYGALYASGHSSVRWSRVLRPLLLVNVTEGQQLRRAFRSIRNALPQILVVF---CLFIFSLLIFSLMALKLLGKRNLKTIDGTSYFTNYLESVFELYVLLTTANSPDVMMPAYNYSSYFAIFFILYIVINTYTFMSVFL-AVVYNNYKKHLKEEVRQLVKTKRQKLRRAFCLLKENIGTNP-----------VVSQSRWTELVKQVQP--NISTAHRELLWSILDHNNKGHIGKQAFLQIADLLNIQVITLKSRPHPFQ--LWMPALYNSTVSRIIHRIVKHRVFVFVYDAVILVNAVFIGLDEEDPLVSNAEWAFLTLYLLEILLKLYTFEPRAFFARHQFWNWFDTIIILSALLATII-NAALKSSGGYTSRQILDIVFILRVLRLIRVVDTIPSFRAIIDTLTRIGPPMLTFGQLILVVY-YIFAMVGMELFKGKIQVFAANSTDPAREY----CGNPLLKDSAFARNNYCKNNFNDVVSSFILLLELTVVN 620
[Close]
BLAST of two pore calcium channel protein 1 vs. Ensembl Cavefish
Match: tpcn1 (two pore segment channel 1 [Source:NCBI gene;Acc:103033570])

HSP 1 Score: 93.9745 bits (232), Expect = 2.366e-19
Identity = 118/538 (21.93%), Postives = 240/538 (44.61%), Query Frame = 1 
Query:  748 IFITSSNSPDVILPGYSKSSYIGILFLIFYAM-FYFLLIFVYGLIMGNFRYHTKEEMTKLLVGKISLMYDAFVLLAEKIVINEKKNCNELFEKNIIKKETWKNLIARFKKYQNCEEAEEIMYSLLSQTNKTYLMKNEFLQMADLMSIPLTIVTNKFHFFE--KNFWFVFSNKLADFVESKYFNLIVGIVIVINSCCITFTSE------------PYXXXXXXXXXXXXXXXRIYVKGFIRYFHSLWNSFDFVVIVLAFIFWFVENMKLNVQTITFTXXXXXXXXXXXXXXGIAAYHQLLHILGMLIPTISSLITIVFCFIVFYVCIGIWSYQDMFLMN------RSELDYMFNSSSCNSTKLEGSDFGRQHYCILSFNSFISGFLTLCSVSIGNNWHTITEGYELESESMILSRFYFSVFKGVLTYLLLLIVAFVLD--VFLLAYNQKSSLKIEELIKNK-----LNSLKINFISYGEIRKSRLTKTDSTEGSQSISLTRYFKRVKFEETSRIWFRLKAKGARSVEYVSQAMFQEEIE 2277
            + +T++N PDV++P YS++ +  + F+++ ++  YF++  +  ++   F    + +   LL+ K S +  AF LL  +               + +  + +  L+ RF + +       + +  L+Q+  T L   +F ++ ++M +      +  H+F+   +  F+    +   V+SK F   + IV+ +N   I   +             P+  + F  ++ +E++L+I   G + YF S WN FDF V V AF+      +  +++   F +++     L         Y  +L  +  L P ++SL   +  F   +  +G+  + D+   N       ++     N +  N T LE       +Y + +FN+ +S F+TL  +++ NNW+   EG  + S++   SR YF  F  V   ++ +IVAF+LD  VF + Y++K+   +++    K         ++  +   E+ K  L  T+ +   +          V  E++         + +R+   +S  M+QEEI+
Sbjct:  267 VLLTTANFPDVMMPAYSRNRWSCVFFIVYLSIELYFIMNLLLAVVFDTFNGVERVKFKSLLLHKRSAIEHAFQLLVSRQ------------RPDGVSLKQFDGLL-RFYRPRMSARDRFLTFKALNQSGTTMLSLQDFYKIYEVMGLKWKARRSGEHWFDDLPHTAFLIFKGINLVVKSKAFQYAIYIVVAVNGVWILVETNMLNSDFSWSQQVPWNYMVFLTIYGVEVLLKITGLGPVAYFSSGWNLFDFSVTVFAFLGLIA--LAFHMEPFYFIVVLRPLQLLRLFKIK-QRYRNVLDTMFELFPRMASLGLTLIIFYYSFAIVGMEFFADVIYPNCCNTSTVADAYRQINVTIGNRTVLE-----EGYYYLNNFNNILSSFVTLFELTVVNNWYITMEG--VTSQTTHWSRLYFMTFYIVTMVVMTIIVAFILDAFVFRMNYSRKNREPLDDPEDEKGIVFEAEVTRVEALQALELYKHTLVGTNISSLQEMC--------VAIEKSGHPSLVFLGRRSRTKSDLSMKMYQEEIQ 773
[Close]
BLAST of two pore calcium channel protein 1 vs. Ensembl Cavefish
Match: tpcn1 (two pore segment channel 1 [Source:NCBI gene;Acc:103033570])

HSP 1 Score: 82.0333 bits (201), Expect = 1.085e-15
Identity = 113/524 (21.56%), Postives = 225/524 (42.94%), Query Frame = 1 
Query:  748 IFITSSNSPDVILPGYSKSSYIGILFLIFYAM-FYFLLIFVYGLIMGNFRYHTKEEMTKLLVGKISLMYDAFVLLAEKIVINEKKNCNELFEKNIIKKETWKNLIARFKKYQNCEEAEEIMYSLLSQTNKTYLMKNEFLQMADLMSIPLTIVTNKFHFFEKNFWFVFSNKLADFVESKYFNLIVGIVIVINSCCITFTSEPYXXXXXXXXXXXXXXXRIYVKGFIRYFHSLWNSFDFVVIVLAFIFWFVENMKLNVQTITFTXXXXXXXXXXXXXXGIAAYHQLLHILGMLIPTISSLITIVFCFIVFYVCIGIWSYQDMFLMN------RSELDYMFNSSSCNSTKLEGSDFGRQHYCILSFNSFISGFLTLCSVSIGNNWHTITEGYELESESMILSRFYFSVFKGVLTYLLLLIVAFVLD--VFLLAYNQKSSLKIEELIKNK-----LNSLKINFISYGEIRKSRLTKTDSTEGSQSISLTRYFKRVKFEETSRIWFRLKAKGARSVEYVSQAMFQEEIE 2277
            + +T++N PDV++P YS++ +  + F+++ ++  YF++  +  ++   F    + +   LL+ K S +  AF LL  +               + +  + +  L+ RF + +       + +  L+Q+  T L    +    DL      I     H       FV S  ++  +  + F   + + +++ SC + +  E              ++L+I   G + YF S WN FDF V V AF+      +  +++   F +++     L         Y  +L  +  L P ++SL   +  F   +  +G+  + D+   N       ++     N +  N T LE       +Y + +FN+ +S F+TL  +++ NNW+   EG  + S++   SR YF  F  V   ++ +IVAF+LD  VF + Y++K+   +++    K         ++  +   E+ K  L  T+ +   +          V  E++         + +R+   +S  M+QEEI+
Sbjct:  102 VLLTTANFPDVMMPAYSRNRWSCVFFIVYLSIELYFIMNLLLAVVFDTFNGVERVKFKSLLLHKRSAIEHAFQLLVSRQ------------RPDGVSLKQFDGLL-RFYRPRMSARDRFLTFKALNQSGTTMLSGEHWFD--DLPHTAFLIFKGFIHLLS----FV-STLVSALINKRCF---LNMQLLVLSCFVVYGVE--------------VLLKITGLGPVAYFSSGWNLFDFSVTVFAFLGLIA--LAFHMEPFYFIVVLRPLQLLRLFKIK-QRYRNVLDTMFELFPRMASLGLTLIIFYYSFAIVGMEFFADVIYPNCCNTSTVADAYRQINVTIGNRTVLEEG-----YYYLNNFNNILSSFVTLFELTVVNNWYITMEG--VTSQTTHWSRLYFMTFYIVTMVVMTIIVAFILDAFVFRMNYSRKNREPLDDPEDEKGIVFEAEVTRVEALQALELYKHTLVGTNISSLQEMC--------VAIEKSGHPSLVFLGRRSRTKSDLSMKMYQEEIQ 570
[Close]
BLAST of two pore calcium channel protein 1 vs. Ensembl Cavefish
Match: tpcn1 (two pore segment channel 1 [Source:NCBI gene;Acc:103033570])

HSP 1 Score: 65.0846 bits (157), Expect = 2.323e-10
Identity = 86/362 (23.76%), Postives = 160/362 (44.20%), Query Frame = 1 
Query: 1267 VESKYFNLIVGIVIVINSCCITFTSE------------PYXXXXXXXXXXXXXXXRIYVKGFIRYFHSLWNSFDFVVIVLAFIFWFVENMKLNVQTITFTXXXXXXXXXXXXXXGIAAYHQLLHILGMLIPTISSLITIVFCFIVFYVCIGIWSYQDMFLMN------RSELDYMFNSSSCNSTKLEGSDFGRQHYCILSFNSFISGFLTLCSVSIGNNWHTITEGYELESESMILSRFYFSVFKGVLTYLLLLIVAFVLD--VFLLAYNQKSSLKIEELIKNK-----LNSLKINFISYGEIRKSRLTKTDSTEGSQSISLTRYFKRVKFEETSRIWFRLKAKGARSVEYVSQAMFQEEIE 2277
            V+SK F   + IV+ +N   I   +             P+  + F  ++ +E++L+I   G + YF S WN FDF V V AF+      +  +++   F +++     L         Y  +L  +  L P ++SL   +  F   +  +G+  + D+   N       ++     N +  N T LE       +Y + +FN+ +S F+TL  +++ NNW+   EG  + S++   SR YF  F  V   ++ +IVAF+LD  VF + Y++K+   +++    K         ++  +   E+ K  L  T+ +   +          V  E++         + +R+   +S  M+QEEI+
Sbjct:  372 VKSKAFQYAIYIVVAVNGVWILVETNMLNSDFSWSQQVPWNYMVFLTIYGVEVLLKITGLGPVAYFSSGWNLFDFSVTVFAFLGLIA--LAFHMEPFYFIVVLRPLQLLRLFKIK-QRYRNVLDTMFELFPRMASLGLTLIIFYYSFAIVGMEFFADVIYPNCCNTSTVADAYRQINVTIGNRTVLE-----EGYYYLNNFNNILSSFVTLFELTVVNNWYITMEG--VTSQTTHWSRLYFMTFYIVTMVVMTIIVAFILDAFVFRMNYSRKNREPLDDPEDEKGIVFEAEVTRVEALQALELYKHTLVGTNISSLQEMC--------VAIEKSGHPSLVFLGRRSRTKSDLSMKMYQEEIQ 715
[Close]
BLAST of two pore calcium channel protein 1 vs. Ensembl Sea Lamprey
Match: tpcn3 (two pore segment channel 3 [Source:ZFIN;Acc:ZDB-GENE-141215-16])

HSP 1 Score: 161.384 bits (407), Expect = 1.760e-41
Identity = 192/760 (25.26%), Postives = 354/760 (46.58%), Query Frame = 1 
Query:  127 AFQL----ISDASVGRLTNFERTVTCLKYYRIYLSRTFRFIRLIIHLTLVILLMYDASNKDSLSLIAKITIPIECACLSFYAIQLFIVYKFSIKSQFIKDKKHYCXXXXXXXXXXXXXXF----KSRVYQFRISPILRVLLFINSAEQSQXXXXXXXXXXXXXNVLVILPFLITFYFMMVAFIYRYFKINKSEPVFYYNKRPYMQNFFDIVWETLIFITSSNSPDVILPGYSKSSYIGILFLIFYAMFYFLLIFVY-GLIMGNFRYHTKEEMTKLLVGKISLMYDAFVLLAEKIVINEKKNCNELFEKNIIKKETWKNLIARFKKYQNCEEAEEIMYSLLSQTNKT-YLMKNEFLQMADLMSIPLTIVTNKFHFFEKNFWFVFSN----KLADFVESKYFNLIVGIVIVINSCCITFTSEPYXXXXXXXXXXXXXXXRIYVKGFI---RYFHS---LWNSFDFVVIVLAFIFWFVENMKLNVQTITFTX-----XXXXXXXXXXXXXGIAAYHQLLHILGMLIPTISSLITIVFCFIVFYVCIGIWSYQD--MFLMNRSELDYMFNSSSCNSTKLEGSDFGRQHYCILSFNSFISGFLTLCSVSIGNNWHTITEGYELESESMILSRFYFSVFKGVLTYLLLLI-VAFVLDVFLLAYNQKSSLKIEELIKNKLNSLKINFISYGEIRKSRLTKTDSTEGSQSISLTRYFKRVKFEETSRIWFRLKAKGARSVEYVSQAMFQEEIEADLKKMKIYEHYFDS 2322
            AFQL    +SDA   R   F+    C+ YYR+Y          +     + L   +   K  L     +T+ +E  CL  ++ +L  + KF+ +S F KD K+ C  ++I++++ID++++     S  Y  R S  LR L  IN +E  Q+RR  R +R T+  +L +L   + F F +  F    FK+     +     +PY QN+F+I +E  + +T+SNSPDV++P Y+ SS+  I F+I+  +  +L + ++  ++  N+R H K E+   +  K   M  AF +L E+           L E   + +++W  L+       +  +A+ ++   +S    T ++ K  F+++ DL++I +     + H  +     V+S+     L   V  ++F      VI++N+  I              LFL   +L I +K +    + F S    WN FD ++++ A +   + +   + +  T T      L+LR LRLL+++     +  ++H +  + PT+ +   ++  F   +  +G+  +Q    F    S     F    C +  L GS F  + YC  +FN   S F+ L  +++ N WH I  G+   + + + +R YF +F  ++  L++ I +AF+L+ F+L Y      + E  I+ K+  L +  I+  ++  + +   D+ E            +      SRI F++  +  R+V+ + Q MF+EE+  +      YE  +D 
Sbjct:    2 AFQLAAAYVSDAQFARNIPFQTARRCIWYYRLYTRWVTWGPMYVFLALNLALAFLETPAKPQLESPLWVTMLLESLCLVAFSSRLVHLAKFTERSVFWKDPKNICVLVTIILTVIDMVIYVALTHSGYYIVRWSRALRPLFVINFSESRQLRRSFRNIRRTLPEILGVL---VLFLFSIAIFSLMAFKLFGKRHLITGEGKPYFQNYFNIFFELYVLVTTSNSPDVMMPAYNSSSWYAIFFIIYIVVNTYLFMSIFLAVVYNNYRKHLKNEVRNSVFSKRRTMAKAFGILRER-----------LGEGWAVSQQSWMQLVG--MAVPSMSQAQRLLLWQVSDEEHTGHIGKQAFVRLTDLLNIEVIETRGRQHPLQSCAPRVYSSAASQALRHVVHHRFFRYFFDGVILLNAGFIAGDEASLAIRQAEWLFLGLYVLEILLKLYTYEPQTFFSRSNFWNWFDVMIVISALLATIIVHSIQDARPTTSTQILDFLLILRVLRLLRIVESFERFRVVIHTMINIGPTMVTYAGLIMVFYYTFAILGMELFQGKIRFFPAGSADAQPF----CGNPLLNGSKFYNEKYCKNNFNDLSSSFVLLVELTVVNQWHDILPGF--VAVTNVGARVYFILFHMIVVILVINIFIAFILEAFILEYTLDKG-ETESAIEKKIQELGLG-IAEEDVAGNGMVLVDNMETQDQEPRDAEASK---PADSRIMFKISDRRYRTVDTLLQRMFEEELAPE------YEGPYDD 728
[Close]
BLAST of two pore calcium channel protein 1 vs. Ensembl Nematostella
Match: EDO33014 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7STF7])

HSP 1 Score: 82.4185 bits (202), Expect = 2.471e-16
Identity = 71/233 (30.47%), Postives = 117/233 (50.21%), Query Frame = 1 
Query: 1396 RIYVKGFIRYFHSLWNSFDFVVIVLAFIFWFVENMKLNVQTITFTXXXXXXXXXXXXXXGIAAYHQLLHILGMLI---PTISSLITIVFCFIVFYVCIGIWSYQDMFLMNRSELD-YMFNSSSCNSTKLEGSDFGRQHYCILSFNSFISGFLTLCSVSIGNNWHTITEGYELESESMILSRFYFSVFKGVLTYLLL-LIVAFVLDVFLLAYNQKSSLKIEELIKNKLNSLKIN 2079
            + Y  GF  +F   WN FDF+VI  A +   +E   +++Q+    L  L  LR+L+L + I    +   I+G L+   P I++   I+F     Y  +GI  +  + L      D    NS+ C + KL+GSDF +  YC  +F+  +     L  +++ N WH IT+G+ L +     +R YF  F  +   ++L + VAF+L+ F+L Y+  S  K E +I+ K+    I 
Sbjct:  302 KFYTIGFKDFFRGFWNWFDFLVIGAATLALVIEASFISLQSSQSVLDFLMVLRVLRLAKIIGQVRRFQVIIGTLMQIGPAITTYGAIMFILYYIYAIVGIELFGGLILKEAPTNDNSTVNSTFCGNIKLKGSDFYQDRYCSNNFDDILKAMKLLFDLTVVNQWHVITQGFVLVTNKW--ARLYFLSFHMICVIVVLNIFVAFILEAFMLEYS-ISHGKFETVIEKKIEEKGIG 531
[Close]
BLAST of two pore calcium channel protein 1 vs. Ensembl Nematostella
Match: EDO47517 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RM05])

HSP 1 Score: 66.2402 bits (160), Expect = 3.529e-11
Identity = 65/252 (25.79%), Postives = 120/252 (47.62%), Query Frame = 1 
Query: 1411 GFIRYFHSLWNSFDFVVIVLAFIFWFVENMKLNVQTITFTXXXXXXXXXXXXXXGIAAYHQLLHILGMLIPTISSLITIVFCFIVFYVCIGIWSYQDMFLMNRSELDYMFNSSSCNSTKLEGSDFGRQHYCILSFNSFISGFLTLCSVSIGNNWHTITEGYELESESMILSRFYFSVFKGVLTYLLLLIVAFVLDVFL--LAYNQKSS-LKIEELIKNKLNSLKINFISYGEIR--KSRLTKTDSTEGSQSI 2151
            G  +YF S WN FDF+V VL  I     + K    +I F + +     LL        Y  +L  + +L+P +  +  ++ C    Y  IG+  +  + L N            CN T ++ +     +Y + +F+  +  F+TL  +++ NNW  I EG  + + +   SR YF  F  +   ++ ++VAFVL+ FL  + Y ++++  ++E  IK ++N      +SY EIR    +L + +  +  +++
Sbjct:  442 GAKQYFRSGWNCFDFIVTVLGIIGAIGASAK--SFSIAFIVCLRPLRLLLLFKL-KQRYRDVLDTIWVLMPRMFRVALVILCLYYSYGIIGLECFSGLKLKN-----------CCNGTSVDVNYRESGYYYLNNFDDLLHAFVTLFELTVVNNWFIIMEG--VVNVTSDWSRIYFMSFFIITMVVMTIVVAFVLEAFLFRIQYRKRTTNEEVEMAIKTEIN------VSYEEIRALDPQLVEQEGVQEGETV 671
[Close]
BLAST of two pore calcium channel protein 1 vs. Ensembl Medaka
Match: ENSORLT00000016743.2 (pep primary_assembly:ASM223467v1:15:29420882:29424861:-1 gene:ENSORLG00000013354.2 transcript:ENSORLT00000016743.2 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 140.969 bits (354), Expect = 3.792e-34
Identity = 181/767 (23.60%), Postives = 368/767 (47.98%), Query Frame = 1 
Query:   88 GDRISFSQDTLDLAFQLISDASVGRLTNFERTVTCLKYYRIY---LSRTFRFIRLIIHLTLVILLMYDASNKDSLSLIAKITIPIECACLSFYAIQLFIVYKFSIKSQFIKDKKHYCXXXXXXXXXXXXXXF----KSRVYQFRISPILRVLLFINSAEQSQXXXXXXXXXXXXXNVLVILPFLITFYFMMVAFIYRYFKINKSEPVFYYNKRPYMQNFFDIVWETLIFITSSNSPDVILPGYSKSSYIGILFLIFYAMFYFLLIFVY-GLIMGNFRYHTKEEMTKLLVGKISLMYDAFVLLAEKIVINEKKNCNELFEKNIIKKETWKNLIARFKKYQNCEEAEEIMYSLLSQTNKTYLMKNEFLQMADLMSIPLTIVTNKFHFFEKNFWFVFSNKLADF----VESKYFNLIVGIVIVINSCCITFTSEPYXXXXXXXXXXXXXXXRIYVKGFI---RYF---HSLWNSFDFVVIVLAFIFWFVENMKLNVQTITFTXXXXXXXXXXXXXXGIAAYHQLLHIL---GMLIPTISSLITIVFCFIVFYVCIGIWSYQDMFLMNRSELDYMFNSSSCNSTKLEGSDFGRQHYCILSFNSFISGFLTLCSVSIGNNWHTITEGYELESESMILSRFYF-SVFKGVLTYLLLLIVAFVLDVFLLAYNQ-KSSL--KIEEL-IKNKLNSLKINFISYGEIRKSRLTKTDSTEGSQSISLTRYFKRVKFEETSRIWFRLKAKGARSVEYVSQAMFQEEIEADLKKMKIYEH 2310
            G   + +++  +LA   +SDA   R   F+ +   ++ Y +Y   + +   +  ++++L+L I           L L    T+ +E  CL  + +++    K   + +F KD K+ C   +++++L D++++     +  Y  R S +LR LL IN  E  Q+RR  R++R  +  +  +  FL+ F F ++ F     K+     +   + +PY  N+ DIV++  + +T++NSPDV++P Y+ +    I F+++  +  ++ + V+  ++  N++ + KEE+ +L+  K   M  AF +L E+          E   + ++ +  W  LI   +    C    ++++S+    N+ +L K  F+Q+ADL++I +  +  + H F   F   + +  + F    V+ + F +   +++++N+  I    +         +FL   +L I +K ++   R F   H+ WN FD +++V A I   V +       +  +      LRL++++  I  +  +++ L   G  I T   LI +V+ +I   + + I+  +  F  + S+ D  +    C +  L+G+ F   +YC  +FN+ +S F+ L  +++ N WH ++ G+   + + + +R +F      V+  ++ + VAFVL+ F L Y+  ++SL  KIEEL +  +   L  + ++  E   + L   +S   S+                S + F++ +K  R+V+    A+ Q   EADL    + E+
Sbjct:   25 GGNAAKTREDFELAAIYVSDAQYNRNIYFDTSPQAVRLYLLYHHWILKMLLYFFIVLNLSLAIF-----EEPAVLPLPTWATMLVELFCLLAFTLRMGHYAKVIPRDKFWKDPKNLCVIATLVLTLTDMVIYGALKAADCYGVRWSRVLRPLLLINVTEGRQLRRAFRSIRNALPQIFYV--FLL-FMFSILLFSLMALKLLGKRDLKTIDGKPYFTNYMDIVFDLYVLVTTANSPDVMMPAYNANVGFAIFFILYILINTYIFMSVFLAVVYNNYKKYLKEEVRQLVRAKRHKMVRAFAVLQEQ---------REEGGQPVVTRNNWNQLIPLVQP-NLCSARRQLIWSVCDDKNQGFLDKVAFIQLADLLNIEVIALKPRSHPFLNLFPNFYQSAPSRFVCKVVQHRGFVIAFDLIVLVNTVFIGLDEKNPLISNSEWVFLTLYLLEILLKLYVFEPRAFFSRHNFWNWFDTIIVVSALIATSVNS------ALGSSGEYTSLLRLVRVVDNIQRFRTIINTLIRIGPAIFTFGQLIVVVY-YIFAMLGMEIFKNKVKFYDDSSDPDKQY----CGNPLLKGTAFANLNYCKNNFNNVVSSFVLLVELTVVNQWHVLSSGF--VTVTNVSARIFFVIFHITVVIIIINIFVAFVLEAFFLEYSDLQTSLEKKIEELNLAMEQERLDNSLVNAMETMDNNLGTEESPTPSR----------------SSMVFKISSKRYRTVD----ALLQRIFEADLAPEDLPEN 740
[Close]
BLAST of two pore calcium channel protein 1 vs. Ensembl Medaka
Match: tpcn3 (two pore calcium channel protein 1 [Source:NCBI gene;Acc:101174889])

HSP 1 Score: 112.464 bits (280), Expect = 3.390e-25
Identity = 174/756 (23.02%), Postives = 342/756 (45.24%), Query Frame = 1 
Query:  109 QDTLDLAFQLISDASVGRLTNFERTVTCLKYYRIY---LSRTFRFIRLIIHLTLVILLMYDASNKDSLSLIAKITIPIECACLSFYAIQLFIVYKFSIKSQFIKDKKHYCXXXXXXXXXXXXXXF----KSRVYQFRISPILRVLLFINSAEQSQXXXXXXXXXXXXXNVLVILPFLITFYFMMVAFIYRYFKINKSEPVFYYNKRPYMQNFFDIVWETLIFITSSNSPDVILPGYSKSSYIGILFLIFYAMFYFLLIFVY-GLIMGNFRYHTKEEMTKLLVGKISLMYDAFVLLAEKIVINEKKNCNELFEKNIIKKETWKNLIARFKKYQNCEEAEEIMYSLLSQTNKTYLMKNEFLQMADLMSIPLTIVTNKFHFFEKNFWFVFSNKLADF----VESKYFNLIVGIVIVINSCCITFTSEPYXXXXXXXXXXXXXXXRIYVKGFI---RYF---HSLWNSFDFVVIVLAFIFWFVENMKLNVQTITFTXXXXXXXXXXXXXXGIAAYHQLLHILGMLIPTISSLITIVFCFIVFYVCIGIWSYQDMFLMNRSELDYMFNSSSCNSTKLEGSDFGRQHYCILSFNSFISGFLTLCSVSIGNNWHTITEGYELESESMILSRFYFSVFKGVLTYLLLLIVAFVLDVFLLAYNQ-KSSL--KIEEL-IKNKLNSLKINFISYGEIRKSRLTKTDSTEGSQSISLTRYFKRVKFEETSRIWFRLKAKGARSVEYVSQAMFQEEIEADLKKMKIYEH 2310
            QD  +LA   +SDA   R   F+ +   ++ Y +Y   + +   +  ++++L+L I           L L    T+ +E  CL  + +++    K   + +F KD K+ C   +++++L D++++     +  Y  R S +LR LL IN  E  Q+RR  R++R  +  +  +  FL+ F F ++ F     K+     +     +PY  N+ DIV++  + +T++NSPDV++P Y+ +    I F+++  +  ++ + V+  ++  N++ + KEE+ +L+  K   M  AF +L E+          E   + ++ +  W  LI   +    C    ++++S+    N+ +L K  F+Q+ADL++I +  +  + H F   F   + +  + F    V+ + F +   ++I++N+  I    +         +FL   +L I +K ++   R F   H+ WN    + IV       +  +  N+Q                        + L+ I G  I T   LI +V+ +I   + + I+  +  F  + S+ D  +    C +  L+G+ F   +YC  +FN+ +S F+ L  +++ N          + +       F+      V+  ++ + VAFVL+ F L Y+  ++SL  KIEEL +  +   L  + ++  E   + L   +S   S+                S + F++ +K  R+V+    A+ Q   EADL    + E+
Sbjct:   16 QD-FELAAIYVSDAQYNRNIYFDTSPQAVRLYLLYHHWILKVLLYFFIVLNLSLAIF-----EEPAVLPLPTWATMLVELFCLLAFTLRMGHYAKVIPRDKFWKDPKNLCIIATLVLTLTDMVIYGALKAADCYGVRWSRVLRPLLLINVTEGRQLRRAFRSIRNALPQIFYV--FLL-FMFSILLFSLMALKLLGKRDLKTIYGKPYFTNYMDIVFDLYVLVTTANSPDVMMPAYNANVGFAIFFILYILINTYIFMSVFLAVVYNNYKKYLKEEVRQLVRAKRHKMVRAFAVLQEQ---------REEGGQPVVTRNNWNQLIPLVQP-NLCSARRQLIWSVCDDKNQGFLDKVAFIQLADLLNIEVIALKPRSHPFLNLFPNFYQSAPSRFVCKVVQHRGFVIAFDLIILVNTVFIGLDEKNPLISNSEWVFLTLYLLEILLKLYVFEPRAFFSRHNFWNCRQILDIVFILRVLRLVRVVDNIQRFR------------------TIINTLIRI-GPAIFTFGQLIVVVY-YIFAMLGMEIFKNKVKFYDDSSDPDKQY----CGNPLLKGTAFANLNYCKNNFNNVVSSFVLLVELTVVNQCSGFVTVTNVSARI-----FFVIFHITVVIIIINIFVAFVLEAFFLEYSDLQTSLEKKIEELNLAMEQERLDNSLVNAMETMDNNLGTEESPTPSR----------------SSMVFKISSKRYRTVD----ALLQRIFEADLAPEDLPEN 703
[Close]
BLAST of two pore calcium channel protein 1 vs. Ensembl Medaka
Match: tpcn1 (two pore segment channel 1 [Source:NCBI gene;Acc:101159752])

HSP 1 Score: 86.6557 bits (213), Expect = 4.598e-17
Identity = 122/544 (22.43%), Postives = 239/544 (43.93%), Query Frame = 1 
Query:  706 PYMQNFFDIVWETLIFITSSNSPDVILPGYSKSSYIGILFLIFYAM-FYFLLIFVYGLIMGNFRYHTKEEMTKLLVGKISLMYDAFVLLAEKIVINEKKNCNELFEKNIIKKETWKNLIARFKKYQNCEEAEEIMYSLLSQTNKTYLMKNEFLQMADLMSIPLTIVTNKFHFFE--KNFWFVFSNKLADFVESKYFNLIVGIVIVINSCCI---TFTSE---------PYXXXXXXXXXXXXXXXRIYVKGFIRYFHSLWNSFDFVVIVLAFIFWFVENMKLNVQTITFTXXXXXXXXXXXXXXGIAAYHQLLHILGMLIPTISSLITIVFCFIVFYVCIGIWSYQDMFLMNRSELDYMFNS-SSCNSTKLEGSDFGRQHYCILSFNSFISGFLTLCSVSIGNNWHTITEGYELESESMILSRFYFSVFKGVLTYLLLLIVAFVLDVFLLAYNQKSSLKIEELIKNKLNSLKINF---ISYGEIRKS-RLTKTDSTEGSQSISLTRYFKRVKFEETSRIWFRLKAKGARSVEYVSQAMFQEEIE 2277
            PY     + +    + +T++N PDV++P YSK+S+  + F+++ ++  YF++  +  ++   F    K +   LL+ K S +  AF LL  +             ++ +       + + R+ + +       + Y  L+ +    L   +F +  +++ +      +  H+F+   +  F+    +   V+SK F   + +V+ IN   I   T+T           P++ + F  ++ +E++L+I   G I YF S WN FDF V + AF+      +  N +   F +++     L         Y  +L  +  L P ++SL   +  F   +  +G+  +  +   N      + +S    N T    +     +Y + +FN+ +  F+TL  +++ NNW+   EG  + S +   SR YF  F  V   ++ +IVAF+LD F+   N   S K  E ++N  +   I F   +S  E   +  L +      S   SLT   + +     S + +  + + +R+   +S  M++EEI+
Sbjct:  256 PYFNTLENSLVSLFVLLTTANFPDVMMPAYSKNSWSCVFFIVYLSIELYFIMNLLLAVVFDTFNDVEKMKFKSLLLHKRSAIDHAFQLLVSR-------------QRPMGVSLKQFDGLMRYYRPRMSARDRFLTYKALNTSGSRMLSLQDFYKFYEVIGLKWKARRSGEHWFDDLPHTAFLIFKGINLLVKSKAFQYAMYVVVAINGVWILVETYTLNSGYSWSKFVPWSYIVFLTIYGVEVLLKIAGLGPINYFSSGWNLFDFSVTLFAFLGLIA--LAFNREPFYFIVVLRPLQLLRLFKIK-QRYRNVLDTMFELFPRMASLGLTLIIFYYSFAIVGMEFFAGVVYPNCCNTSTVADSYRQINVTHGNTTVLEEGYYYLNNFNNILRSFVTLFELTVVNNWYITMEG--VTSMTSHWSRLYFMTFYIVTMVVMTIIVAFILDAFVFRMNY--SRKNREPVENPEDENGIVFEVEVSRDEAMTTLELYRQTCLTPSSLSSLTGVLQAMDRSGHSSLVY--QGRRSRTKSDLSMKMYEEEIQ 777
[Close]
BLAST of two pore calcium channel protein 1 vs. Ensembl Medaka
Match: tpcn1 (two pore segment channel 1 [Source:NCBI gene;Acc:101159752])

HSP 1 Score: 77.7962 bits (190), Expect = 1.788e-14
Identity = 115/521 (22.07%), Postives = 222/521 (42.61%), Query Frame = 1 
Query:  772 PDVILPGYSKSSYIGILFLIFYAM-FYFLLIFVYGLIMGNFRYHTKEEMTKLLVGKISLMYDAFVLLAEKIVINEKKNCNELFEKNIIKKETWKNLIARFKKYQNCEEAEEIMYSLLSQTNKTYLMKNEFLQMADLMSIPLTIVTNKFHFFE--KNFWFVFSNKLADFVESKYFNLIVGIVIVINSCCI---TFTSE---------PYXXXXXXXXXXXXXXXRIYVKGFIRYFHSLWNSFDFVVIVLAFIFWFVENMKLNVQTITFTXXXXXXXXXXXXXXGIAAYHQLLHILGMLIPTISSLITIVFCFIVFYVCIGIWSYQDMFLMN----RSELDYMFNSSSCNSTKLEGSDFGRQHYCILSFNSFISGFLTLCSVSIGNNWHTITEGYELESESMILSRFYFSVFKGVLTYLLLLIVAFVLDVFLLAYNQKSSLKIEELIKNKLNSLKINFISYGEIRKSRLTKTDSTEGSQSISLTRYFKRVKFEETSRIWFRLKAKGARSVEYVSQAMFQEEIE 2277
            PDV++P YSK+S+  + F+++ ++  YF++  +  ++   F    K +   LL+ K S +  AF LL  +             ++ +       + + R+ + +       + Y  L+ +    L   +F +  +++ +      +  H+F+   +  F+    +   V+SK F   + +V+ IN   I   T+T           P++ + F  ++ +E++L+I   G I YF S WN FDF V + AF+      +  N +   F +++     L         Y  +L  +  L P ++SL   +  F   +  +G+  +  +   N     ++     N +  N+T LE       +Y + +FN+ +  F+TL  +++ NNW+   EG  + S +   SR YF  F  V   ++ +IVAF+LD F+   N           KN  N +        E+  SR     + E  +   LT +                + + +R+   +S  M++EEI+
Sbjct:   49 PDVMMPAYSKNSWSCVFFIVYLSIELYFIMNLLLAVVFDTFNDVEKMKFKSLLLHKRSAIDHAFQLLVSR-------------QRPMGVSLKQFDGLMRYYRPRMSARDRFLTYKALNTSGSRMLSLQDFYKFYEVIGLKWKARRSGEHWFDDLPHTAFLIFKGINLLVKSKAFQYAMYVVVAINGVWILVETYTLNSGYSWSKFVPWSYIVFLTIYGVEVLLKIAGLGPINYFSSGWNLFDFSVTLFAFLGLIA--LAFNREPFYFIVVLRPLQLLRLFKIK-QRYRNVLDTMFELFPRMASLGLTLIIFYYSFAIVGMEFFAGVVYPNCCNTVADSYRQINVTHGNTTVLE-----EGYYYLNNFNNILRSFVTLFELTVVNNWYITMEG--VTSMTSHWSRLYFMTFYIVTMVVMTIIVAFILDAFVFRMNYSR--------KNHENGIVF------EVEVSRDEAMTTLELYRQTCLTSWLTSCACFCPQHSSLVYQGRRSRTKSDLSMKMYEEEIQ 532
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BLAST of two pore calcium channel protein 1 vs. Planmine SMEST
Match: SMESG000045181.1 (SMESG000045181.1)

HSP 1 Score: 513.842 bits (1322), Expect = 1.153e-178
Identity = 287/291 (98.63%), Postives = 288/291 (98.97%), Query Frame = 1 
Query:  712 MQNFFDIVWETLIFITSSNSPDVILPGYSKSSYIGILFLIFYAMFYFLLIFVYGLIMGNFRYHTKEEMTKLLVGKISLMYDAFVLLAEKIVINEKKNCNELFEKNIIKKETWKNLIARFKKYQNCEEAEEIMYSLLSQTNKTYLMKNEFLQMADLMSIPLTIVTNKFHFFEKNFWFVFSNKLADFVESKYFNLIVGIVIVINSCCITFTSEPYXXXXXXXXXXXXXXXRIYVKGFIRYFHSLWNSFDFVVIVLAFIFWFVENMKLNVQTITFTXXXXXXXXXXXXXXGIAA 1584
            MQNFFDIVWETLIFITSSNSPDVILPGYSKSSYIGILFLIFYAMFYFLLIFVYGLIMGNFRYHTKEEMTKLLVGK SLMYDAFVLLAEKIVINEKKNCNE+ EKNIIKKETWKNLIARFKKYQNCEEAEEIMYSLLSQTNKTYLMKNEFLQMADLMSIPLTIVTNKFHFFEKNFWFVFSNKLADFVESKYFNLIVGIVIVINSCCITFTSEPYTELFFTILFLLEIILRIYVKGFIRYFHSLWN FDFVVIVLAFIFWFVENMKLNVQTITFTLLVLRNLRLLKLLRGIAA
Sbjct:    1 MQNFFDIVWETLIFITSSNSPDVILPGYSKSSYIGILFLIFYAMFYFLLIFVYGLIMGNFRYHTKEEMTKLLVGKTSLMYDAFVLLAEKIVINEKKNCNEVIEKNIIKKETWKNLIARFKKYQNCEEAEEIMYSLLSQTNKTYLMKNEFLQMADLMSIPLTIVTNKFHFFEKNFWFVFSNKLADFVESKYFNLIVGIVIVINSCCITFTSEPYTELFFTILFLLEIILRIYVKGFIRYFHSLWNIFDFVVIVLAFIFWFVENMKLNVQTITFTLLVLRNLRLLKLLRGIAA 291
[Close]
BLAST of two pore calcium channel protein 1 vs. Planmine SMEST
Match: SMESG000045168.1 (SMESG000045168.1)

HSP 1 Score: 501.516 bits (1290), Expect = 4.317e-174
Identity = 264/266 (99.25%), Postives = 266/266 (100.00%), Query Frame = 1 
Query:  712 MQNFFDIVWETLIFITSSNSPDVILPGYSKSSYIGILFLIFYAMFYFLLIFVYGLIMGNFRYHTKEEMTKLLVGKISLMYDAFVLLAEKIVINEKKNCNELFEKNIIKKETWKNLIARFKKYQNCEEAEEIMYSLLSQTNKTYLMKNEFLQMADLMSIPLTIVTNKFHFFEKNFWFVFSNKLADFVESKYFNLIVGIVIVINSCCITFTSEPYXXXXXXXXXXXXXXXRIYVKGFIRYFHSLWNSFDFVVIVLAFIFWFVENMKLN 1509
            MQNFFDIVWETLIFITSSNSPDVILPGYSKSSYIGILFLIFYAMFYFLLIFVYGLIMGNFRYHTKEEMTKLLVGKISLMYDAFVLLAEKIVINEKKNCNE+FEKNIIKKETWKNLIARFKKYQNCEEAEEIMYSLLSQTNKTYLMKNEFLQMADLMSIPLTIVTNKFHFFEKNFWFVFSNKLADFVESKYFNLIVGIVIVINSCCITFTSEPYTELFFTILFLLEIILRIYVKGFIRYFHSLWNSFDFVVIVLAFIFWFVENMKL+
Sbjct:    1 MQNFFDIVWETLIFITSSNSPDVILPGYSKSSYIGILFLIFYAMFYFLLIFVYGLIMGNFRYHTKEEMTKLLVGKISLMYDAFVLLAEKIVINEKKNCNEVFEKNIIKKETWKNLIARFKKYQNCEEAEEIMYSLLSQTNKTYLMKNEFLQMADLMSIPLTIVTNKFHFFEKNFWFVFSNKLADFVESKYFNLIVGIVIVINSCCITFTSEPYTELFFTILFLLEIILRIYVKGFIRYFHSLWNSFDFVVIVLAFIFWFVENMKLS 266
[Close]
BLAST of two pore calcium channel protein 1 vs. Planmine SMEST
Match: SMESG000045178.1 (SMESG000045178.1)

HSP 1 Score: 157.918 bits (398), Expect = 1.795e-45
Identity = 77/81 (95.06%), Postives = 80/81 (98.77%), Query Frame = 1 
Query:   88 GDRISFSQDTLDLAFQLISDASVGRLTNFERTVTCLKYYRIYLSRTFRFIRLIIHLTLVILLMYDASNKDSLSLIAKITIP 330
            GDRISFSQDTLDLAFQLISDASVGRLTNFERTVTCLKYYRIYLSRTFRFIRLIIHLTLVILLMYDASNKDSLSLIAK+++ 
Sbjct:   11 GDRISFSQDTLDLAFQLISDASVGRLTNFERTVTCLKYYRIYLSRTFRFIRLIIHLTLVILLMYDASNKDSLSLIAKVSLK 91
[Close]
BLAST of two pore calcium channel protein 1 vs. Planmine SMEST
Match: SMESG000024145.1 (SMESG000024145.1)

HSP 1 Score: 148.288 bits (373), Expect = 1.198e-36
Identity = 160/628 (25.48%), Postives = 291/628 (46.34%), Query Frame = 1 
Query:  490 QFRISPILRVLLFINSAEQSQXXXXXXXXXXXXXNVLVILPFLITFYFMMVAFIYRYFKINKSEPVFYYNKRPYMQNFFDIVWETLIFITSSNSPDVILPGYSKSSYIGILFLIFYAMFYFLLIFVYGLIMGNFRYHTKEEMTKLLVGKISLMYDAF-VLLAEKIVINEKKNCNELFEKNIIKKET------WKNLIARFKKYQN--CEEAEEIMYSLLSQTNKTYLMKNEFLQMADLMSIPLTIVTNKFHFFEKN----FWF---VFSNKLADFVESKYFNLIVGIVIVINSCC-ITFTSEPYXXX---XXXXXXXXXXXXRIYVKGFIRYFHSLWNSFDFVVIVLAFIFWFVE------NMKLNVQTITFTXXXXXXXXXXXXXXGIAAYHQLLHILGMLIPTISSLITIVFCFIVFYVCIGIWSYQDMFLMNRSELDYMFNSS---SCNSTKLEGSDFGRQHYCILSFNSFISGFLTLCSVSIGNNWHTITEGYELESESMILSRFYFSVFKGVLTYLLLLIVAFVLDVFLLAYNQKSSLKIEELIKNKLNSLKINFISYGEIRKSRLTKTDSTEGSQSISLTRYFKRVKFE---ETSRIWFRLKAKGARSVEYVSQAMFQEEIE 2277
            +F+ S  LR +L     E  +IR  L  L+ T+ ++L+I      F FM+  F     +   S  ++Y N  PY +N+ D++++  + +T+SNSPDVI+P Y KS+   IL+L+       + I    + MG                 I+ +Y+A+ V L   ++ N K+  + L E   + K +        +  A  KK      E    +++ LL       L   +F ++ + M +    +   + FF K     +W     F+ K+   + SKY  L    V+++N+   I F    +       FT++F  E+++R++  G   YF  +WN FDFVVI+LA I  F++      NM ++   + F L +     L  L   I ++  ++  + +L+P++ +   +  C    YVC+G+  + + +      +  M NS+    C   +L+ SDF + HYCIL FNS   GF  +  +++ NNWH +  G  L +       F+ S+    +  ++ +++AF++D +L+          E +I+ +L  ++I   +  E+RK  L       G+ +  L++      F    E+  I FRL     + +E + Q MF+++I+
Sbjct:  156 RFQWSRFLRPVLCATFPENHKIRSALNNLKGTLHDILIIFGL---FSFMLGFFSLVALQFLASADLYYPNGIPYFRNYLDVIFDLYVLVTTSNSPDVIIPAYEKSTSYSILYLL-------VTIVCNWIFMG---------------ITIASVYNAYKVHLGRGVINNVKQKKHRLKEAFYLLKSSDTGEVEMSSFCAVMKKVDPSYSESKNILLFKLLDINKSNSLTVGQFTKLTEYMHLNFEEIEMNYGFFNKKIPKLYWCYMSSFNQKIKPIIRSKYTRLFFEGVVLLNAILMIIFNDTEWELTFEWVFTLIFSGELLIRMFASGVNLYFDEMWNVFDFVVILLALISLFIQQIISYFNMHISNHYLRFLLALRVLRLLKILTV-IRSFRIIIQSIFILLPSLLAYTAVQLCIYYIYVCVGMEVFGNRYFPPYGNIYEMKNSTDTVDCGDPRLKNSDFTKSHYCILGFNSASDGFFLMTILTVSNNWHILLLGPVLTTHKAA-KLFFVSIHLICVILVMNIVLAFIIDAYLMEVESNKG-HFENVIEERL--VEIGLSANVELRKKNLQGY----GTINFDLSKEMLHNAFPNSNESGSICFRLNESTPKDMETLLQRMFEDDIQ 749
The following BLAST results are available for this feature:
BLAST of two pore calcium channel protein 1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 4
Match NameE-valueIdentityDescription
TPCN15.838e-1822.40two pore segment channel 1 [Source:HGNC Symbol;Acc... [more]
TPCN11.509e-1722.40two pore segment channel 1 [Source:HGNC Symbol;Acc... [more]
TPCN17.059e-1722.40two pore segment channel 1 [Source:HGNC Symbol;Acc... [more]
TPCN17.059e-1722.40two pore segment channel 1 [Source:HGNC Symbol;Acc... [more]
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BLAST of two pore calcium channel protein 1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of two pore calcium channel protein 1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of two pore calcium channel protein 1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 4
Match NameE-valueIdentityDescription
tpcn32.912e-3726.06two pore segment channel 3 [Source:ZFIN;Acc:ZDB-GE... [more]
tpcn11.670e-1622.62two pore segment channel 1 [Source:ZFIN;Acc:ZDB-GE... [more]
tpcn14.478e-1422.62two pore segment channel 1 [Source:ZFIN;Acc:ZDB-GE... [more]
tpcn11.536e-927.59two pore segment channel 1 [Source:ZFIN;Acc:ZDB-GE... [more]
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BLAST of two pore calcium channel protein 1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSXETT00000032923.12.607e-3324.08two pore calcium channel protein 1-like [Source:NC... [more]
ENSXETT00000018477.11.721e-3224.24two pore calcium channel protein 1-like [Source:NC... [more]
ENSXETT00000032877.12.129e-3124.36two pore calcium channel protein 1-like [Source:NC... [more]
ENSXETT00000032915.14.372e-2324.10two pore calcium channel protein 1-like [Source:NC... [more]
ENSXETT00000032851.14.492e-2324.63two pore calcium channel protein 1-like [Source:NC... [more]
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BLAST of two pore calcium channel protein 1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Tpcn13.678e-2222.87two pore channel 1 [Source:MGI Symbol;Acc:MGI:2182... [more]
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BLAST of two pore calcium channel protein 1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q9EQJ0|TPC1_MOUSE2.573e-2122.87Two pore calcium channel protein 1 OS=Mus musculus... [more]
sp|Q9WTN5|TPC1_RAT2.256e-2022.69Two pore calcium channel protein 1 OS=Rattus norve... [more]
sp|Q9ULQ1|TPC1_HUMAN7.248e-1722.40Two pore calcium channel protein 1 OS=Homo sapiens... [more]
sp|Q94KI8|TPC1_ARATH7.880e-1422.05Two pore calcium channel protein 1 OS=Arabidopsis ... [more]
sp|Q75VR1|TPC1A_TOBAC3.992e-1223.83Two pore calcium channel protein 1A OS=Nicotiana t... [more]
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BLAST of two pore calcium channel protein 1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A2C9KPG91.030e-4125.33Uncharacterized protein OS=Biomphalaria glabrata O... [more]
A0A267F8Q14.535e-4026.98Uncharacterized protein (Fragment) OS=Macrostomum ... [more]
A0A1I8J6U55.402e-4026.98Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A2B4SLZ36.133e-3924.64Two pore calcium channel protein 1 OS=Stylophora p... [more]
A0A1I8G1898.216e-3926.58Uncharacterized protein OS=Macrostomum lignano OX=... [more]
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BLAST of two pore calcium channel protein 1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 4
Match NameE-valueIdentityDescription
tpcn34.569e-2525.48two pore segment channel 3 [Source:ZFIN;Acc:ZDB-GE... [more]
tpcn12.366e-1921.93two pore segment channel 1 [Source:NCBI gene;Acc:1... [more]
tpcn11.085e-1521.56two pore segment channel 1 [Source:NCBI gene;Acc:1... [more]
tpcn12.323e-1023.76two pore segment channel 1 [Source:NCBI gene;Acc:1... [more]
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BLAST of two pore calcium channel protein 1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
tpcn31.760e-4125.26two pore segment channel 3 [Source:ZFIN;Acc:ZDB-GE... [more]
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BLAST of two pore calcium channel protein 1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of two pore calcium channel protein 1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 2
Match NameE-valueIdentityDescription
EDO330142.471e-1630.47Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO475173.529e-1125.79Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of two pore calcium channel protein 1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 4
Match NameE-valueIdentityDescription
ENSORLT00000016743.23.792e-3423.60pep primary_assembly:ASM223467v1:15:29420882:29424... [more]
tpcn33.390e-2523.02two pore calcium channel protein 1 [Source:NCBI ge... [more]
tpcn14.598e-1722.43two pore segment channel 1 [Source:NCBI gene;Acc:1... [more]
tpcn11.788e-1422.07two pore segment channel 1 [Source:NCBI gene;Acc:1... [more]
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BLAST of two pore calcium channel protein 1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 4
Match NameE-valueIdentityDescription
SMESG000045181.11.153e-17898.63SMESG000045181.1[more]
SMESG000045168.14.317e-17499.25SMESG000045168.1[more]
SMESG000045178.11.795e-4595.06SMESG000045178.1[more]
SMESG000024145.11.198e-3625.48SMESG000024145.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30028664 ID=SMED30028664|Name=two pore calcium channel protein 1|organism=Schmidtea mediterranea sexual|type=transcript|length=2350bp
CTTATGAATATAAATTTAATTTGCATATAATTATATGAATTTCTAGATTT
CAATGAAATGAACAATTTAGAATTAAATAATCTTGATGGAGATAGAATCA
GCTTTTCTCAGGATACATTAGATTTAGCATTCCAGTTGATATCTGATGCA
TCAGTTGGACGCCTCACTAATTTTGAAAGAACTGTAACTTGTCTAAAATA
TTACCGTATATATCTTAGTAGAACTTTTCGATTTATAAGACTTATTATTC
ACTTAACTTTGGTAATACTTTTGATGTATGATGCATCAAACAAAGATTCT
CTATCATTAATAGCTAAGATAACTATACCTATCGAATGCGCATGTTTGAG
TTTTTATGCAATACAATTATTTATTGTATACAAATTTTCAATAAAATCTC
AATTTATCAAGGACAAAAAGCACTACTGTTCGGGACTGTCAATATTAGTC
AGCCTCATTGACATCATTTTATTCAAGAGTCGGGTTTATCAATTCCGCAT
CAGTCCAATTCTTAGGGTGCTATTATTTATTAATTCGGCCGAGCAATCAC
AGATAAGGAGACATTTACGAACTTTGAGAATAACAATAATAAATGTATTG
GTAATATTGCCATTTTTAATAACCTTTTATTTTATGATGGTGGCCTTCAT
TTATCGCTACTTCAAAATCAACAAATCTGAACCTGTGTTTTATTACAATA
AAAGGCCGTACATGCAGAATTTTTTTGATATTGTTTGGGAAACATTAATA
TTTATTACATCTTCCAACAGTCCCGATGTCATATTACCGGGATATTCAAA
ATCTTCCTATATTGGAATCCTATTTCTCATTTTCTATGCAATGTTTTATT
TTCTTTTGATTTTTGTGTACGGTTTAATAATGGGAAATTTTCGGTATCAT
ACCAAAGAAGAAATGACAAAACTACTAGTGGGGAAAATTTCTCTAATGTA
CGATGCGTTCGTACTACTTGCTGAAAAGATCGTGATAAACGAGAAAAAAA
ACTGCAATGAATTGTTTGAGAAGAATATAATAAAAAAAGAGACTTGGAAG
AACCTAATTGCAAGATTTAAAAAATATCAAAACTGCGAGGAAGCCGAAGA
GATTATGTATTCGTTGTTGTCTCAAACAAATAAAACATATTTGATGAAAA
ATGAATTTTTACAAATGGCTGATCTCATGTCTATTCCTCTGACAATAGTT
ACGAACAAATTTCATTTTTTCGAAAAGAATTTTTGGTTTGTTTTCAGTAA
TAAATTGGCAGATTTTGTTGAAAGCAAGTATTTCAACTTAATTGTTGGCA
TAGTAATTGTAATAAATTCCTGTTGCATAACATTTACCAGTGAGCCGTAC
ACAGAATTATTCTTCACTATATTATTTCTCCTGGAAATCATTTTACGTAT
TTATGTGAAAGGGTTTATCAGATATTTCCACAGTTTGTGGAACAGCTTTG
ATTTCGTGGTCATTGTGCTGGCATTCATATTTTGGTTTGTGGAAAATATG
AAATTAAATGTTCAAACCATTACTTTTACTCTCCTGGTGCTGAGAAATTT
GCGATTGCTAAAATTATTAAGAGGGATAGCTGCCTACCATCAACTGTTGC
ACATATTGGGAATGCTTATTCCAACGATATCCAGTCTGATAACAATTGTT
TTTTGTTTCATTGTATTTTATGTTTGTATTGGGATTTGGTCATATCAGGA
TATGTTTCTCATGAACCGTTCTGAACTGGATTATATGTTCAATTCCTCCA
GCTGTAACAGCACAAAATTGGAAGGCAGTGATTTCGGCCGACAACATTAT
TGTATATTGTCTTTCAACTCTTTCATTTCAGGATTTCTCACCCTGTGCTC
GGTATCAATTGGAAATAATTGGCACACAATTACCGAGGGCTACGAATTGG
AATCGGAGAGCATGATCCTCAGTCGGTTTTATTTCTCCGTATTCAAAGGC
GTCCTGACGTACCTGTTGCTACTCATAGTTGCTTTTGTATTGGACGTATT
TTTACTTGCCTACAATCAGAAAAGCTCTTTGAAAATTGAAGAGTTAATTA
AAAATAAATTAAATTCCCTAAAAATCAATTTCATTTCTTATGGTGAAATT
AGAAAATCCAGGCTAACCAAAACTGACTCGACCGAAGGTTCACAGTCAAT
ATCGCTTACCCGTTATTTTAAACGGGTGAAATTCGAAGAAACAAGTCGAA
TTTGGTTTCGTTTGAAGGCAAAAGGAGCGAGGTCAGTTGAATATGTCAGC
CAAGCCATGTTCCAAGAAGAAATCGAGGCCGATTTGAAGAAAATGAAGAT
TTATGAACATTATTTTGACTCATAATAAAATCCTAATTTTGAAAAAAAAA
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protein sequence of SMED30028664-orf-1

>SMED30028664-orf-1 ID=SMED30028664-orf-1|Name=SMED30028664-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=756bp
MNNLELNNLDGDRISFSQDTLDLAFQLISDASVGRLTNFERTVTCLKYYR
IYLSRTFRFIRLIIHLTLVILLMYDASNKDSLSLIAKITIPIECACLSFY
AIQLFIVYKFSIKSQFIKDKKHYCSGLSILVSLIDIILFKSRVYQFRISP
ILRVLLFINSAEQSQIRRHLRTLRITIINVLVILPFLITFYFMMVAFIYR
YFKINKSEPVFYYNKRPYMQNFFDIVWETLIFITSSNSPDVILPGYSKSS
YIGILFLIFYAMFYFLLIFVYGLIMGNFRYHTKEEMTKLLVGKISLMYDA
FVLLAEKIVINEKKNCNELFEKNIIKKETWKNLIARFKKYQNCEEAEEIM
YSLLSQTNKTYLMKNEFLQMADLMSIPLTIVTNKFHFFEKNFWFVFSNKL
ADFVESKYFNLIVGIVIVINSCCITFTSEPYTELFFTILFLLEIILRIYV
KGFIRYFHSLWNSFDFVVIVLAFIFWFVENMKLNVQTITFTLLVLRNLRL
LKLLRGIAAYHQLLHILGMLIPTISSLITIVFCFIVFYVCIGIWSYQDMF
LMNRSELDYMFNSSSCNSTKLEGSDFGRQHYCILSFNSFISGFLTLCSVS
IGNNWHTITEGYELESESMILSRFYFSVFKGVLTYLLLLIVAFVLDVFLL
AYNQKSSLKIEELIKNKLNSLKINFISYGEIRKSRLTKTDSTEGSQSISL
TRYFKRVKFEETSRIWFRLKAKGARSVEYVSQAMFQEEIEADLKKMKIYE
HYFDS*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:0005509calcium ion binding
GO:0005216ion channel activity
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000020protonephridia
PLANA:0000026gut
PLANA:0003116parenchymal cell
PLANA:0007528glial cell
Vocabulary: INTERPRO
TermDefinition
IPR027359Volt_channel_dom_sf
IPR005821Ion_trans_dom
Vocabulary: biological process
TermDefinition
GO:0055085transmembrane transport
GO:0006811ion transport
GO:0034220ion transmembrane transport
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005821Ion transport domainPFAMPF00520Ion_transcoord: 335..576
e-value: 3.3E-21
score: 75.6
IPR027359Voltage-dependent channel domain superfamilyGENE3DG3DSA:1.20.120.350coord: 325..438
e-value: 1.1E-15
score: 59.7
NoneNo IPR availablePANTHERPTHR46726:SF1TWO-PORE CALCIUM CHANNEL 3coord: 14..610
NoneNo IPR availablePANTHERPTHR46726FAMILY NOT NAMEDcoord: 14..610
NoneNo IPR availableSUPERFAMILYSSF81324Voltage-gated potassium channelscoord: 334..523
NoneNo IPR availableTMHMMTMhelixcoord: 13..35
NoneNo IPR availableTMHMMTMhelixcoord: 508..530
NoneNo IPR availableTMHMMTMhelixcoord: 385..407
NoneNo IPR availableTMHMMTMhelixcoord: 336..355
NoneNo IPR availableTMHMMTMhelixcoord: 180..202
NoneNo IPR availableTMHMMTMhelixcoord: 50..67
NoneNo IPR availableTMHMMTMhelixcoord: 452..474
NoneNo IPR availableTMHMMTMhelixcoord: 559..581
NoneNo IPR availableTMHMMTMhelixcoord: 359..378
NoneNo IPR availableTMHMMTMhelixcoord: 105..127