SMED30028618

Overview
NameSMED30028618
Smed IDSMED30028618
Length (bp)1770
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30028618 (SMED30028618) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30028618 (SMED30028618) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30028618 (SMED30028618) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30028618 (SMED30028618) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Homology
BLAST of SMED30028618 vs. Planmine SMEST
Match: SMESG000071379.1 (SMESG000071379.1)

HSP 1 Score: 605.905 bits (1561), Expect = 0.000e+0
Identity = 331/331 (100.00%), Postives = 331/331 (100.00%), Query Frame = 3
Query:    6 MLPNQIPSALHLFNIETIEKKNNCREILGLKKVNNSFGGLYGIDFFLEDAPTHKNNYVPCLENGCSFRGPSSMMEFHVTTVHEAGLNIQFDTDTKVWINDRKKNFPNSTVCKSQPEKSIXXXXXXXXXXXXXXXXXPVKQESREPVDKDPMLAILEKKSISEGNDLLGLGAMYGSSSDEEEIVQPNQLSNEFXXXXXXXXXXXXXXXKRRNKFKFPILPQMELQSKRNALMNPQRRYHETFNRKAKRPRLADLLLAEDIISERKMVLQQLRYIVNKRFFDSSLLSIPITDLKPESGHDANDDVIEEEFFTSISDGETENIDSIEWSDDDCM 998
            MLPNQIPSALHLFNIETIEKKNNCREILGLKKVNNSFGGLYGIDFFLEDAPTHKNNYVPCLENGCSFRGPSSMMEFHVTTVHEAGLNIQFDTDTKVWINDRKKNFPNSTVCKSQPEKSIKQFNTKNKTKNQTKFQKPVKQESREPVDKDPMLAILEKKSISEGNDLLGLGAMYGSSSDEEEIVQPNQLSNEFLGLPLPSELPPHLLLKRRNKFKFPILPQMELQSKRNALMNPQRRYHETFNRKAKRPRLADLLLAEDIISERKMVLQQLRYIVNKRFFDSSLLSIPITDLKPESGHDANDDVIEEEFFTSISDGETENIDSIEWSDDDCM
Sbjct:    1 MLPNQIPSALHLFNIETIEKKNNCREILGLKKVNNSFGGLYGIDFFLEDAPTHKNNYVPCLENGCSFRGPSSMMEFHVTTVHEAGLNIQFDTDTKVWINDRKKNFPNSTVCKSQPEKSIKQFNTKNKTKNQTKFQKPVKQESREPVDKDPMLAILEKKSISEGNDLLGLGAMYGSSSDEEEIVQPNQLSNEFLGLPLPSELPPHLLLKRRNKFKFPILPQMELQSKRNALMNPQRRYHETFNRKAKRPRLADLLLAEDIISERKMVLQQLRYIVNKRFFDSSLLSIPITDLKPESGHDANDDVIEEEFFTSISDGETENIDSIEWSDDDCM 331          
BLAST of SMED30028618 vs. Planmine SMEST
Match: SMESG000071379.1 (SMESG000071379.1)

HSP 1 Score: 255.758 bits (652), Expect = 7.950e-83
Identity = 119/119 (100.00%), Postives = 119/119 (100.00%), Query Frame = 3
Query:    6 MLPNQIPSALHLFNIETIEKKNNCREILGLKKVNNSFGGLYGIDFFLEDAPTHKNNYVPCLENGCSFRGPSSMMEFHVTTVHEAGLNIQFDTDTKVWINDRKKNFPNSTVCKSQPEKSI 362
            MLPNQIPSALHLFNIETIEKKNNCREILGLKKVNNSFGGLYGIDFFLEDAPTHKNNYVPCLENGCSFRGPSSMMEFHVTTVHEAGLNIQFDTDTKVWINDRKKNFPNSTVCKSQPEKSI
Sbjct:    1 MLPNQIPSALHLFNIETIEKKNNCREILGLKKVNNSFGGLYGIDFFLEDAPTHKNNYVPCLENGCSFRGPSSMMEFHVTTVHEAGLNIQFDTDTKVWINDRKKNFPNSTVCKSQPEKSI 119          
The following BLAST results are available for this feature:
BLAST of SMED30028618 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028618 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028618 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028618 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028618 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028618 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028618 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028618 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028618 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028618 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028618 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028618 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028618 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028618 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000071379.10.000e+0100.00SMESG000071379.1[more]
SMESG000071379.17.950e-83100.00SMESG000071379.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30028618 ID=SMED30028618|Name=SMED30028618|organism=Schmidtea mediterranea sexual|type=transcript|length=1770bp
TTTAAATGCTACCGAATCAAATTCCTTCAGCTTTACACCTCTTCAATATT
GAAACGATTGAAAAGAAAAATAACTGTAGAGAGATTCTTGGACTAAAGAA
AGTTAATAATTCGTTTGGTGGTCTTTATGGAATAGATTTTTTTCTGGAAG
ATGCTCCGACTCACAAAAATAACTACGTTCCTTGCTTAGAGAACGGCTGT
TCATTTCGTGGACCTTCTTCTATGATGGAATTTCACGTCACAACTGTTCA
TGAGGCTGGGTTGAATATTCAATTTGATACCGATACTAAAGTTTGGATAA
ACGATCGCAAGAAAAATTTCCCAAATTCAACTGTTTGCAAGTCTCAACCG
GAAAAGTCTATTAAGCAATTTAATACAAAAAATAAGACAAAAAACCAAAC
AAAATTTCAAAAGCCAGTTAAACAAGAATCCAGGGAACCAGTAGATAAGG
ATCCAATGTTAGCTATATTGGAAAAGAAATCTATTTCCGAAGGAAATGAT
TTATTGGGCTTGGGGGCTATGTATGGATCTAGTAGTGATGAAGAAGAAAT
CGTTCAACCAAATCAGTTGTCAAATGAATTTTTAGGATTACCATTGCCCT
CAGAATTGCCTCCGCATTTATTATTGAAACGACGTAATAAATTTAAATTT
CCCATCCTTCCTCAAATGGAGTTACAATCGAAACGCAACGCATTAATGAA
TCCCCAAAGAAGATATCATGAAACGTTCAACAGGAAGGCAAAACGTCCCA
GATTAGCTGATTTGTTACTAGCTGAAGATATTATATCAGAAAGAAAAATG
GTGCTGCAGCAACTCAGATATATTGTCAATAAACGGTTCTTTGATTCTTC
TCTTCTAAGTATTCCAATAACCGACTTGAAACCTGAATCTGGGCACGATG
CAAATGATGACGTAATAGAGGAAGAGTTTTTCACAAGCATCAGCGACGGA
GAAACTGAAAACATTGACTCTATTGAATGGAGTGATGATGATTGCATGTA
TCCTCAACATATCTCCTCTCTTTAAAAGAAATTCGTCCTCGAATTAAATT
TGACACTCAATTCCATTTTGATCATGCTCTACAACTCTTTATAACTTTAC
ATTAATTCAATTATCAATAAAAATAACAACTGCTTTTAAAACTCTTCATA
CAACTTATTGTTTAAAAATTTAACATGCAAATGTGTAATGTAATTTTTTA
TGTTATTTATAAATTTCATGAAATTTGCGCCTATGCAAATGAAATAAATT
AAACGATCTCCACTGATTTACAAAAATATTTACTATTCACTTTCAAAATT
CTTAATTTCTTCACAATTTTCTGAAATCTAGAGTAGTCGAAGGTTTTGAC
CCATTGTAACTTTTTATCAAAATTTCACACGATTATAGTTCTATAGTAAT
TTTTGATCGTCTTATTATTCTTCACAACATATCAAAAATAAGAATTTTTG
GCCAAATTTGTAACCCTTTATAACTTTTTATTTAAAGCTTTGATGAATAA
AGTTATATTGGCAATTTTTCTTGTTTTGTCGATCTCTACAACATATCAAA
AATTAGAAATTTTGTCCAAGGTTTTGACCCTTTATAACTTTTGACCCAGA
AGCCATTCTTCTATAGTAAATATTGCTCAGCTTTTCAAGCTCTACCACAT
ATTAAAAATTAGAATTTTTGAAGGTTTGACCCTTTATAACGTCTTATCCA
AAGTTTGAAAATCATAATTTTATCGTCACTTTTGTCATATTATCGTTCTC
TACAACATATCAAAAATAAA
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protein sequence of SMED30028618-orf-1

>SMED30028618-orf-1 ID=SMED30028618-orf-1|Name=SMED30028618-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=340bp
MLPNQIPSALHLFNIETIEKKNNCREILGLKKVNNSFGGLYGIDFFLEDA
PTHKNNYVPCLENGCSFRGPSSMMEFHVTTVHEAGLNIQFDTDTKVWIND
RKKNFPNSTVCKSQPEKSIKQFNTKNKTKNQTKFQKPVKQESREPVDKDP
MLAILEKKSISEGNDLLGLGAMYGSSSDEEEIVQPNQLSNEFLGLPLPSE
LPPHLLLKRRNKFKFPILPQMELQSKRNALMNPQRRYHETFNRKAKRPRL
ADLLLAEDIISERKMVLQQLRYIVNKRFFDSSLLSIPITDLKPESGHDAN
DDVIEEEFFTSISDGETENIDSIEWSDDDCMYPQHISSL*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002109X1 cell
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 108..133
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 108..145
NoneNo IPR availablePANTHERPTHR13309NUCLEAR FRAGILE X MENTAL RETARDATION PROTEIN INTERACTING PROTEIN 1coord: 50..293
NoneNo IPR availablePANTHERPTHR13309:SF0NUCLEAR FRAGILE X MENTAL RETARDATION-INTERACTING PROTEIN 1coord: 50..293