SMED30028565

Overview
NameSMED30028565
Smed IDSMED30028565
Length (bp)324
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30028565 (SMED30028565) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30028565 (SMED30028565) in cells (dots) of each of the 12 neoblast clusters.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30028565

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 11

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
prepharyngeal regionSMED30028565 SmedASXL_015592SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30028565 SmedASXL_015592SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
prepharyngeal regionSMED30028565 SmedASXL_075607SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30028565 SmedASXL_075607SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30028565 dd_2dd_Smed_v4PMID:29674431
Fincher et al., 2018		whole organism asexual adult fluorescence in situ hybridization evidence
parenchymaSMED30028565 dd_2dd_Smed_v4PMID:29674431
Fincher et al., 2018		whole organism asexual adult fluorescence in situ hybridization evidence
parenchymaSMED30028565 dd_Smed_v4_2_1_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parapharyngeal regionSMED30028565 dd_Smed_v6_2_1_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
head regionSMED30028565 OX_Smed_1.0.18828ox_Smed_v2PMID:24238224
Kao et al., 2013		whole organism asexual adult RNA-sequencing evidence
head regionSMED30028565 OX_Smed_1.0.17157ox_Smed_v2PMID:24238224
Kao et al., 2013		whole organism asexual adult RNA-sequencing evidence
head regionSMED30028565 OX_Smed_1.0.20111ox_Smed_v2PMID:24238224
Kao et al., 2013		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
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BLAST of SMED30028565 vs. Planmine SMEST
Match: SMESG000067659.1 (SMESG000067659.1)

HSP 1 Score: 166.392 bits (420), Expect = 4.274e-55
Identity = 84/84 (100.00%), Postives = 84/84 (100.00%), Query Frame = -1 
Query:   73 MKLFIFFLGLIVVAMCEDCLDSGDDCASKTGEDCTNEDVYTKCPKSCHFCGDCADSRPDCAFLKERGDCDKMDDRVCRKTCGGC 324
            MKLFIFFLGLIVVAMCEDCLDSGDDCASKTGEDCTNEDVYTKCPKSCHFCGDCADSRPDCAFLKERGDCDKMDDRVCRKTCGGC
Sbjct:    1 MKLFIFFLGLIVVAMCEDCLDSGDDCASKTGEDCTNEDVYTKCPKSCHFCGDCADSRPDCAFLKERGDCDKMDDRVCRKTCGGC 84
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BLAST of SMED30028565 vs. Planmine SMEST
Match: SMESG000067661.1 (SMESG000067661.1)

HSP 1 Score: 161.77 bits (408), Expect = 2.555e-53
Identity = 81/84 (96.43%), Postives = 82/84 (97.62%), Query Frame = -1 
Query:   73 MKLFIFFLGLIVVAMCEDCLDSGDDCASKTGEDCTNEDVYTKCPKSCHFCGDCADSRPDCAFLKERGDCDKMDDRVCRKTCGGC 324
            MKLFIFFLGL+VVAMCEDCLDSGDDCASKTGEDCTNEDV TKCPKSCH CGDCADSRPDCAFLKERGDCDKMDDRVCRKTCGGC
Sbjct:    1 MKLFIFFLGLVVVAMCEDCLDSGDDCASKTGEDCTNEDVLTKCPKSCHLCGDCADSRPDCAFLKERGDCDKMDDRVCRKTCGGC 84
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BLAST of SMED30028565 vs. Planmine SMEST
Match: SMESG000067668.1 (SMESG000067668.1)

HSP 1 Score: 160.999 bits (406), Expect = 5.287e-53
Identity = 81/84 (96.43%), Postives = 82/84 (97.62%), Query Frame = -1 
Query:   73 MKLFIFFLGLIVVAMCEDCLDSGDDCASKTGEDCTNEDVYTKCPKSCHFCGDCADSRPDCAFLKERGDCDKMDDRVCRKTCGGC 324
            MKLFIFFLGLIVVAMCEDCLDSGDDCASKTGEDCTNE+V TKCPKSCH CGDCADSRPDCAFLKERGDCDKMDDRVCRKTCGGC
Sbjct:    1 MKLFIFFLGLIVVAMCEDCLDSGDDCASKTGEDCTNEEVLTKCPKSCHLCGDCADSRPDCAFLKERGDCDKMDDRVCRKTCGGC 84
[Close]
BLAST of SMED30028565 vs. Planmine SMEST
Match: SMESG000079865.1 (SMESG000079865.1)

HSP 1 Score: 148.673 bits (374), Expect = 3.476e-48
Identity = 75/84 (89.29%), Postives = 78/84 (92.86%), Query Frame = -1 
Query:   73 MKLFIFFLGLIVVAMCEDCLDSGDDCASKTGEDCTNEDVYTKCPKSCHFCGDCADSRPDCAFLKERGDCDKMDDRVCRKTCGGC 324
            MKLFI  LGLIVVA CEDCLDSGDDCASKTGEDCT+E+VYTKCPKSCHFCGDCADSRPDC FLKERGDC+ MDDRVCRKTCG C
Sbjct:    1 MKLFIVLLGLIVVAKCEDCLDSGDDCASKTGEDCTSEEVYTKCPKSCHFCGDCADSRPDCDFLKERGDCELMDDRVCRKTCGKC 84
[Close]
BLAST of SMED30028565 vs. Planmine SMEST
Match: SMESG000067683.1 (SMESG000067683.1)

HSP 1 Score: 137.887 bits (346), Expect = 5.314e-44
Identity = 70/70 (100.00%), Postives = 70/70 (100.00%), Query Frame = -1 
Query:   73 MCEDCLDSGDDCASKTGEDCTNEDVYTKCPKSCHFCGDCADSRPDCAFLKERGDCDKMDDRVCRKTCGGC 282
            MCEDCLDSGDDCASKTGEDCTNEDVYTKCPKSCHFCGDCADSRPDCAFLKERGDCDKMDDRVCRKTCGGC
Sbjct:    1 MCEDCLDSGDDCASKTGEDCTNEDVYTKCPKSCHFCGDCADSRPDCAFLKERGDCDKMDDRVCRKTCGGC 70
The following BLAST results are available for this feature:
BLAST of SMED30028565 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028565 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028565 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028565 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028565 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028565 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028565 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028565 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028565 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028565 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028565 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028565 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028565 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028565 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000067659.14.274e-55100.00SMESG000067659.1[more]
SMESG000067661.12.555e-5396.43SMESG000067661.1[more]
SMESG000067668.15.287e-5396.43SMESG000067668.1[more]
SMESG000079865.13.476e-4889.29SMESG000079865.1[more]
SMESG000067683.15.314e-44100.00SMESG000067683.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30028565 ID=SMED30028565|Name=SMED30028565|organism=Schmidtea mediterranea sexual|type=transcript|length=324bp
AGACATAAATACAAATGTAATTTTTCAACTCGTCGTTTTCAATATACATT
GAAAGCAATCATTTTTGATTTAACAACCTCCACAAGTTTTTCTGCAAACT
CTGTCGTCCATTTTGTCACAATCTCCTCGTTCTTTCAAAAATGCGCAGTC
AGGTCGGCTGTCAGCGCAATCTCCACAGAAGTGACAGGATTTTGGACATT
TGGTATAAACGTCTTCATTGGTACAATCTTCACCAGTTTTCGATGCGCAA
TCATCTCCACTGTCGAGGCAGTCTTCACACATGGCAACGACGATCAAACC
CAAGAAGAAAATGAACAATTTCAT
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protein sequence of SMED30028565-orf-1

>SMED30028565-orf-1 ID=SMED30028565-orf-1|Name=SMED30028565-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=108bp
RHKYKCNFSTRRFQYTLKAIIFDLTTSTSFSANSVVHFVTISSFFQKCAV
RSAVSAISTEVTGFWTFGINVFIGTIFTSFRCAIISTVEAVFTHGNDDQT
QEENEQFH
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000418head
PLANA:0000420parapharyngeal region
PLANA:0002142parenchyma
Vocabulary: INTERPRO
TermDefinition
IPR003582ShKT_dom
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003582ShKT domainSMARTSM00254ShkT_1coord: 52..84
e-value: 14.0
score: 8.3
coord: 18..51
e-value: 0.08
score: 22.1
IPR003582ShKT domainPFAMPF01549ShKcoord: 53..84
e-value: 6.9E-4
score: 20.1
coord: 19..50
e-value: 0.011
score: 16.3
IPR003582ShKT domainPROSITEPS51670SHKTcoord: 53..84
score: 6.787
IPR003582ShKT domainPROSITEPS51670SHKTcoord: 19..50
score: 7.658
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..16
score: 0.861