SMED30028468

Overview
NameSMED30028468
Smed IDSMED30028468
Length (bp)1106
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30028468 (SMED30028468) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30028468 (SMED30028468) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30028468 (SMED30028468) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30028468 (SMED30028468) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30028468

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
oocyteSMED30028468 Contig19737uc_Smed_v2PMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite RNA-sequencing evidence
oocyteSMED30028468 Contig19737newmark_estsPMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite RNA-sequencing evidence
vitelline glandSMED30028468 Contig19737uc_Smed_v2PMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite RNA-sequencing evidence
vitelline glandSMED30028468 Contig19737newmark_estsPMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of SMED30028468 vs. Planmine SMEST
Match: SMESG000051017.1 (SMESG000051017.1)

HSP 1 Score: 214.542 bits (545), Expect = 6.435e-86
Identity = 118/190 (62.11%), Postives = 145/190 (76.32%), Query Frame = 2
Query:    8 MILPSNS-SLIYPDNNPSTFVVEFENPVYLDGKWEVALMDFTFVYNHFPYCSNSCIQYKFKKSHKETKYLKXXXXXXXXXXXXSKYTPDVDVFMENYENLLIVCPHVPSKISFRDYEHASKFGLQNISITSESDHCIIKLPKFMENNAVEQIEIIINYDIKTKFTHYFEDDLFLTRLSELHGYLMTINAS 574
            MILPSNS  LIYP+N  S F V+  N VYLDG+WEVAL+DFTFVYN FPY SNSCI+Y+  KS  E KYLKINN+N   EI+N KYTPDVD+F+E YENL+IVC HVP K++F D +HA  FGL++ SI  E DHC IKLPKF ++  +EQ  III+Y+ K+KF  YFED+LF+  LSEL G+L++ N S
Sbjct:  244 MILPSNSCPLIYPNNEASNFNVQV-NSVYLDGEWEVALIDFTFVYNPFPYYSNSCIRYESVKSGSEIKYLKINNQNHTYEIINPKYTPDVDMFIEKYENLVIVCSHVPFKVTFPDIQHAFSFGLKSTSIICEEDHCRIKLPKFTDDGVMEQFGIIISYEEKSKFEKYFEDNLFINTLSELQGHLLSFNPS 432          

HSP 2 Score: 122.479 bits (306), Expect = 6.435e-86
Identity = 77/116 (66.38%), Postives = 98/116 (84.48%), Query Frame = 1
Query:  577 LKKSILEMKNQTFTYKDLVGVKIPKIIQIHQ-IFNYSNIIKPINVGNVTVPLLKSVWIKKHEQGEVVQLEMKXXXXXXXXXXXXXXXXXXVPDDSGKLIKFSKDIKTHLTLHFRKI 921
            L KS L +K   F+ K+++G KIPK++  HQ +F YSNII+PINVG+V VPLLKS+WIKK++QGE+VQLEMKNP+Y+ +S S+IN IE+N+ DDSGKLIKFSKD KTHL+LHFRKI
Sbjct:  462 LAKS-LGLKLSIFSEKNILGTKIPKLLHHHQQMFIYSNIIEPINVGDVRVPLLKSIWIKKYDQGEIVQLEMKNPMYLPVSYSTINIIEINIRDDSGKLIKFSKDTKTHLSLHFRKI 576          

HSP 3 Score: 107.457 bits (267), Expect = 4.087e-51
Identity = 47/57 (82.46%), Postives = 51/57 (89.47%), Query Frame = 2
Query:  935 DIVNFYRISQSGGDLPYFVSKQYGTGWLKTIGQFALPILKRLRNFGMKTTNDVINNN 1105
            DIVNFY   Q+GGDLPYFV KQYG+GWLKTIG+FALPILKR+ NFGMKT NDVINNN
Sbjct:  860 DIVNFYSTHQNGGDLPYFVGKQYGSGWLKTIGRFALPILKRIGNFGMKTANDVINNN 916          

HSP 4 Score: 75.485 bits (184), Expect = 4.087e-51
Identity = 49/159 (30.82%), Postives = 83/159 (52.20%), Query Frame = 2
Query:   86 VYLDGKWEVALMDFTFVYNHFPYCSNSCIQYKFKKSHKETKYLKXXXXXXXXXXXXSKYTPDVDVFMENYENLLIVCPHVPSKISFRDYEHASKFGLQNISITSESDHCIIKLPKFMENNAVEQIEIIINYDIKTKFTHYFEDDLFLTRLSELHGYLMT 562
            +YLDG+WEVAL +FTFVYN+FP+ +NSCI+Y     H   +Y      N  +   ++ Y     ++++N + L I C + P K++F   E A+  G     +        I++P   +    + I + I Y    ++T  F+++LF+T  S++   LM 
Sbjct:  576 IYLDGEWEVALTEFTFVYNNFPFYNNSCIKYTKSTLHNSYEYFYYIEGNYTISHNSTFYL----IYIDN-KILNINCKYFPFKVTFDSIEEANCLGFNKKEVDFNDKLNQIRIPN--KKTKFDTIRLTITYYYDVEYTETFKENLFITEFSQVTDQLMA 727          

HSP 5 Score: 58.9214 bits (141), Expect = 4.087e-51
Identity = 46/94 (48.94%), Postives = 70/94 (74.47%), Query Frame = 1
Query:  592 LEMKNQTFTYKDLVGVKIPKIIQIH-QIFNYSNIIKPINVGNVTVPLLKSVWIKKHEQGEVVQLEMKXXXXXXXXXXXXXXXXXXVPDDSGKLI 870
            L +  ++++ K +VG  +P I+Q + Q+  Y+NII+PI V  ++VPLLK VWI+KH++ ++VQ   KNP+Y+ +SSS INNIE+N+ DDSGK I
Sbjct:  765 LGLIEESYSEKIIVGSSLPTIMQRNIQMMIYTNIIEPILVSELSVPLLKCVWIEKHKKDDLVQFNEKNPMYLPVSSSYINNIEINIRDDSGKYI 858          
BLAST of SMED30028468 vs. Planmine SMEST
Match: SMESG000018599.1 (SMESG000018599.1)

HSP 1 Score: 209.534 bits (532), Expect = 2.089e-85
Identity = 116/190 (61.05%), Postives = 142/190 (74.74%), Query Frame = 2
Query:    8 MILPSNSS-LIYPDNNPSTFVVEFENPVYLDGKWEVALMDFTFVYNHFPYCSNSCIQYKFKKSHKETKYLKXXXXXXXXXXXXSKYTPDVDVFMENYENLLIVCPHVPSKISFRDYEHASKFGLQNISITSESDHCIIKLPKFMENNAVEQIEIIINYDIKTKFTHYFEDDLFLTRLSELHGYLMTINAS 574
            MILPSNS  LIYP+N  S F V+  N VYLDG+WEVAL+DFTFVYN FPY SNSCI+Y+  KS  E KYLKINN+N   EI+N KYTPDVD+F+E YENL+IVCPHVP K++F D +HA  FGL++ SI  E D C IKLPKF ++  +EQ  I INY+ K+ F  Y ED+LF+  LSEL G+L++ N S
Sbjct:    8 MILPSNSCPLIYPNNEASNFNVQV-NSVYLDGEWEVALIDFTFVYNPFPYYSNSCIRYESVKSGSEIKYLKINNQNHTYEIINPKYTPDVDMFIEKYENLVIVCPHVPFKVTFPDIQHAFSFGLKSTSIICEEDQCRIKLPKFTDDGVMEQFGININYEEKSIFEKYLEDNLFINTLSELQGHLLSFNPS 196          

HSP 2 Score: 125.946 bits (315), Expect = 2.089e-85
Identity = 78/116 (67.24%), Postives = 99/116 (85.34%), Query Frame = 1
Query:  577 LKKSILEMKNQTFTYKDLVGVKIPKIIQIHQ-IFNYSNIIKPINVGNVTVPLLKSVWIKKHEQGEVVQLEMKXXXXXXXXXXXXXXXXXXVPDDSGKLIKFSKDIKTHLTLHFRKI 921
            L KS LE+K   F+ K+++G KIPK++  HQ +F YSNII+PINVG+V VPLLKS+WIKK++QGE+VQLEMKNP+Y+ +S S+IN IE+N+ DDSGKLIKFSKD KTHL+LHFRKI
Sbjct:  226 LAKS-LELKLSNFSEKNILGTKIPKLLHHHQQMFIYSNIIEPINVGDVRVPLLKSIWIKKYDQGEIVQLEMKNPMYLPVSYSTINIIEINIRDDSGKLIKFSKDTKTHLSLHFRKI 340          

HSP 3 Score: 79.337 bits (194), Expect = 1.139e-15
Identity = 51/159 (32.08%), Postives = 88/159 (55.35%), Query Frame = 2
Query:   86 VYLDGKWEVALMDFTFVYNHFPYCSNSCIQYKFKKSHKETKYLKXXXXXXXXXXXXSKYTPDVDVFMENYENLLIVCPHVPSKISFRDYEHASKFGLQNISITSESDHCIIKLPKFMENNAVEQIEIIINYDIKTKFTHYFEDDLFLTRLSELHGYLMT 562
            +YLDG+WEVAL +FTFVYN+FP+ +NSCI+Y     H   +Y      N  +   ++ Y     ++++N + L I C + P K++F   E A+  G     +    +   I++P   + +  + I + I+Y    ++T  F+++LF+T LS++   LMT
Sbjct:  340 IYLDGEWEVALTEFTFVYNNFPFYNNSCIKYTKSTLHNSYEYFYYIEGNYTISHNSTFYL----IYIDN-KILNINCKYFPFKVTFDSIEEANCLGFNKKEVDFNYELNQIRIPN--KKSKFDTIRLTISYYYDVEYTETFKENLFITELSQVTDQLMT 491          
BLAST of SMED30028468 vs. Planmine SMEST
Match: SMESG000069427.1 (SMESG000069427.1)

HSP 1 Score: 211.846 bits (538), Expect = 2.926e-84
Identity = 117/190 (61.58%), Postives = 144/190 (75.79%), Query Frame = 2
Query:    8 MILPSNSS-LIYPDNNPSTFVVEFENPVYLDGKWEVALMDFTFVYNHFPYCSNSCIQYKFKKSHKETKYLKXXXXXXXXXXXXSKYTPDVDVFMENYENLLIVCPHVPSKISFRDYEHASKFGLQNISITSESDHCIIKLPKFMENNAVEQIEIIINYDIKTKFTHYFEDDLFLTRLSELHGYLMTINAS 574
            MILPSNS  LIYP+N  S F V+  N VYLDG+WEVAL+DFTFVYN FPY SNSCI+Y+  KS  E KYLKINN+N   EI+N KYTPDVD+F+E YENL+IVCPHVP K++F D +HA  FGL++ SI  E DHC IKLPKF ++  +EQ  I I+Y+ K+ F  YFED+LF+  LSEL G+L++ N S
Sbjct:  207 MILPSNSCPLIYPNNEASNFNVQV-NSVYLDGEWEVALIDFTFVYNPFPYYSNSCIRYESVKSGSEIKYLKINNQNHTYEIINPKYTPDVDMFIEKYENLVIVCPHVPFKVTFPDIQHAFSFGLKSTSIICEEDHCRIKLPKFTDDGVMEQFGINISYEEKSIFEKYFEDNLFINTLSELQGHLLSFNPS 395          

HSP 2 Score: 119.783 bits (299), Expect = 2.926e-84
Identity = 71/106 (66.98%), Postives = 91/106 (85.85%), Query Frame = 1
Query:  607 QTFTYKDLVGVKIPKIIQIHQ-IFNYSNIIKPINVGNVTVPLLKSVWIKKHEQGEVVQLEMKXXXXXXXXXXXXXXXXXXVPDDSGKLIKFSKDIKTHLTLHFRKI 921
              ++ K++ G KIPK++  HQ +F YSNII+PINVG+V VPLLKS+WIKK++QGE+VQLEMKNP+Y+ +S S+IN IE+N+ DDSGKLIKFSKD KTHL+LHFRKI
Sbjct:  434 SNYSEKNIFGTKIPKLLHHHQQMFIYSNIIEPINVGDVRVPLLKSIWIKKYDQGEIVQLEMKNPMYLPVSYSTINIIEINIRDDSGKLIKFSKDTKTHLSLHFRKI 539          

HSP 3 Score: 102.834 bits (255), Expect = 7.747e-34
Identity = 47/57 (82.46%), Postives = 51/57 (89.47%), Query Frame = 2
Query:  935 DIVNFYRISQSGGDLPYFVSKQYGTGWLKTIGQFALPILKRLRNFGMKTTNDVINNN 1105
            DIVNFY   Q+GGDLPYFV KQYG+GWLKTIG+FALPILKR+ NFGMKT NDVINNN
Sbjct:  823 DIVNFYSTHQNGGDLPYFVGKQYGSGWLKTIGRFALPILKRIGNFGMKTANDVINNN 879          

HSP 4 Score: 60.077 bits (144), Expect = 7.747e-34
Identity = 46/89 (51.69%), Postives = 69/89 (77.53%), Query Frame = 1
Query:  607 QTFTYKDLVGVKIPKIIQIH-QIFNYSNIIKPINVGNVTVPLLKSVWIKKHEQGEVVQLEMKXXXXXXXXXXXXXXXXXXVPDDSGKLI 870
            ++++ K +VG  +P I+Q + Q+  Y+NII+PI VG ++VPLLK VWI+KH++ ++VQ   KNP+Y+ +SSS INNIE+N+ DDSGK I
Sbjct:  733 ESYSEKIIVGSSLPTIMQRNIQMMIYTNIIEPILVGELSVPLLKCVWIEKHKKDDLVQFIEKNPMYLPVSSSYINNIEINIRDDSGKYI 821          

HSP 5 Score: 74.3294 bits (181), Expect = 4.071e-14
Identity = 50/159 (31.45%), Postives = 87/159 (54.72%), Query Frame = 2
Query:   86 VYLDGKWEVALMDFTFVYNHFPYCSNSCIQYKFKKSHKETKYLKXXXXXXXXXXXXSKYTPDVDVFMENYENLLIVCPHVPSKISFRDYEHASKFGLQNISITSESDHCIIKLPKFMENNAVEQIEIIINYDIKTKFTHYFEDDLFLTRLSELHGYLMT 562
            +YLDG+WEVAL +FTFVYN+FP+ +NS I+Y     H   +Y      N  +   ++ Y     ++++N + L I C + P K++F   E A+  G     +    +   I++P   + + ++ I + I Y    ++T  F+++LF+T LS++   LMT
Sbjct:  539 IYLDGEWEVALTEFTFVYNNFPFYNNSHIKYIKSTLHNSYEYFYYIEGNYTISHNSTFYL----IYIDN-KILNINCKYFPFKVTFDSIEEANCLGFNKKEVDFNDELNQIRIPN--KKSKLDTIRLTITYYYDVEYTETFKENLFITELSQVTDQLMT 690          
BLAST of SMED30028468 vs. Planmine SMEST
Match: SMESG000072420.1 (SMESG000072420.1)

HSP 1 Score: 211.846 bits (538), Expect = 3.285e-84
Identity = 117/190 (61.58%), Postives = 144/190 (75.79%), Query Frame = 2
Query:    8 MILPSNSS-LIYPDNNPSTFVVEFENPVYLDGKWEVALMDFTFVYNHFPYCSNSCIQYKFKKSHKETKYLKXXXXXXXXXXXXSKYTPDVDVFMENYENLLIVCPHVPSKISFRDYEHASKFGLQNISITSESDHCIIKLPKFMENNAVEQIEIIINYDIKTKFTHYFEDDLFLTRLSELHGYLMTINAS 574
            MILPSNS  LIYP+N  S F V+  N VYLDG+WEVAL+DFTFVYN FPY SNSCI+Y+  KS  E KYLKINN+N   EI+N KYTPDVD+F+E YENL+IVCPHVP K++F D +HA  FGL++ SI  E DHC IKLPKF ++  +EQ  I I+Y+ K+ F  YFED+LF+  LSEL G+L++ N S
Sbjct:  324 MILPSNSCPLIYPNNEASNFNVQV-NSVYLDGEWEVALIDFTFVYNPFPYYSNSCIRYESVKSGSEIKYLKINNQNHTYEIINPKYTPDVDMFIEKYENLVIVCPHVPFKVTFPDIQHAFSFGLKSTSIICEEDHCRIKLPKFTDDGVMEQFGINISYEEKSIFEKYFEDNLFINTLSELQGHLLSFNPS 512          

HSP 2 Score: 119.783 bits (299), Expect = 3.285e-84
Identity = 75/115 (65.22%), Postives = 96/115 (83.48%), Query Frame = 1
Query:  577 LKKSILEMKNQTFTYKDLVGVKIPKIIQIHQ-IFNYSNIIKPINVGNVTVPLLKSVWIKKHEQGEVVQLEMKXXXXXXXXXXXXXXXXXXVPDDSGKLIKFSKDIKTHLTLHFRK 918
            L KS L +K   ++ K++ G KIPK++  HQ +F YSNII+PINVG+V VPLLKS+WIKK++QGE+VQLEMKNP+Y+ +S S+IN IE+N+ DDSGKLIKFSKD KTHL+LHFRK
Sbjct:  542 LAKS-LGLKLSNYSEKNIFGTKIPKLLHHHQQMFIYSNIIEPINVGDVRVPLLKSIWIKKYDQGEIVQLEMKNPMYLPVSYSTINIIEINIRDDSGKLIKFSKDTKTHLSLHFRK 655          

HSP 3 Score: 77.0258 bits (188), Expect = 5.470e-32
Identity = 33/41 (80.49%), Postives = 36/41 (87.80%), Query Frame = 2
Query:  983 YFVSKQYGTGWLKTIGQFALPILKRLRNFGMKTTNDVINNN 1105
            Y   KQYG+GWLKTIG+FALPILKR+ NFGMKT NDVINNN
Sbjct:  904 YIFGKQYGSGWLKTIGRFALPILKRIGNFGMKTANDVINNN 944          

HSP 4 Score: 60.077 bits (144), Expect = 5.470e-32
Identity = 47/94 (50.00%), Postives = 71/94 (75.53%), Query Frame = 1
Query:  592 LEMKNQTFTYKDLVGVKIPKIIQIH-QIFNYSNIIKPINVGNVTVPLLKSVWIKKHEQGEVVQLEMKXXXXXXXXXXXXXXXXXXVPDDSGKLI 870
            L +  ++++ K +VG  +P I+Q + Q+  Y+NII+PI VG ++VPLLK VWI+KH++ ++VQ   KNP+Y+ +SSS INNIE+N+ DDSGK I
Sbjct:  812 LGLIEESYSEKIIVGSSLPTIMQRNIQMMIYTNIIEPILVGELSVPLLKCVWIEKHKKDDLVQFIEKNPMYLPVSSSYINNIEINIRDDSGKYI 905          

HSP 5 Score: 39.6614 bits (91), Expect = 5.470e-32
Identity = 24/87 (27.59%), Postives = 45/87 (51.72%), Query Frame = 2
Query:  302 LLIVCPHVPSKISFRDYEHASKFGLQNISITSESDHCIIKLPKFMENNAVEQIEIIINYDIKTKFTHYFEDDLFLTRLSELHGYLMT 562
            L I C + P K++F   E A+  G     +    +   I++P   + + ++ I + I Y    ++T  F+++LF+T LS++   LMT
Sbjct:  690 LNINCKYFPFKVTFDSIEEANCLGFNKKEVDFNDELNQIRIPN--KKSKLDTIRLTITYYYDVEYTETFKENLFITELSQVTDQLMT 774          
BLAST of SMED30028468 vs. Planmine SMEST
Match: SMESG000052400.1 (SMESG000052400.1)

HSP 1 Score: 211.46 bits (537), Expect = 1.180e-83
Identity = 117/190 (61.58%), Postives = 144/190 (75.79%), Query Frame = 2
Query:    8 MILPSNSS-LIYPDNNPSTFVVEFENPVYLDGKWEVALMDFTFVYNHFPYCSNSCIQYKFKKSHKETKYLKXXXXXXXXXXXXSKYTPDVDVFMENYENLLIVCPHVPSKISFRDYEHASKFGLQNISITSESDHCIIKLPKFMENNAVEQIEIIINYDIKTKFTHYFEDDLFLTRLSELHGYLMTINAS 574
            MILPSNS  LIYP+N  S F V+  N VYLDG+WEVAL+DFTFVYN FPY SNSCI+Y+  KS  E KYLKINN+N   EI+N KYTPDVD+F+E YENL+IVCPHVP K++F D +HA  FGL++ SI  E DHC IKLPKF ++  +EQ  I I+Y+ K+ F  YFED+LF+  LSEL G+L++ N S
Sbjct:  324 MILPSNSCPLIYPNNEASNFNVQV-NSVYLDGEWEVALIDFTFVYNPFPYYSNSCIRYESVKSGSEIKYLKINNQNHTYEIINPKYTPDVDMFIEKYENLVIVCPHVPFKVTFPDIQHAFSFGLKSTSIICEEDHCRIKLPKFTDDGVMEQFGINISYEEKSIFEKYFEDNLFINTLSELQGHLLSFNPS 512          

HSP 2 Score: 118.242 bits (295), Expect = 1.180e-83
Identity = 70/104 (67.31%), Postives = 90/104 (86.54%), Query Frame = 1
Query:  610 TFTYKDLVGVKIPKIIQIHQ-IFNYSNIIKPINVGNVTVPLLKSVWIKKHEQGEVVQLEMKXXXXXXXXXXXXXXXXXXVPDDSGKLIKFSKDIKTHLTLHFRK 918
             ++ K++ G KIPK++  HQ +F YSNII+PINVG+V VPLLKS+WIKK++QGE+VQLEMKNP+Y+ +S S+IN IE+N+ DDSGKLIKFSKD KTHL+LHFRK
Sbjct:  552 NYSEKNIFGTKIPKLLHHHQQMFIYSNIIEPINVGDVRVPLLKSIWIKKYDQGEIVQLEMKNPMYLPVSYSTINIIEINIRDDSGKLIKFSKDTKTHLSLHFRK 655          

HSP 3 Score: 102.449 bits (254), Expect = 2.382e-36
Identity = 47/57 (82.46%), Postives = 51/57 (89.47%), Query Frame = 2
Query:  935 DIVNFYRISQSGGDLPYFVSKQYGTGWLKTIGQFALPILKRLRNFGMKTTNDVINNN 1105
            DIVNFY   Q+GGDLPYFV KQYG+GWLKTIG+FALPILKR+ NFGMKT NDVINNN
Sbjct:  855 DIVNFYSTHQNGGDLPYFVGKQYGSGWLKTIGRFALPILKRIGNFGMKTANDVINNN 911          

HSP 4 Score: 60.077 bits (144), Expect = 2.382e-36
Identity = 47/94 (50.00%), Postives = 71/94 (75.53%), Query Frame = 1
Query:  592 LEMKNQTFTYKDLVGVKIPKIIQIH-QIFNYSNIIKPINVGNVTVPLLKSVWIKKHEQGEVVQLEMKXXXXXXXXXXXXXXXXXXVPDDSGKLI 870
            L +  ++++ K +VG  +P I+Q + Q+  Y+NII+PI VG ++VPLLK VWI+KH++ ++VQ   KNP+Y+ +SSS INNIE+N+ DDSGK I
Sbjct:  760 LGLIEESYSEKIIVGSSLPTIMQRNIQMMIYTNIIEPILVGELSVPLLKCVWIEKHKKDDLVQFIEKNPMYLPVSSSYINNIEINIRDDSGKYI 853          

HSP 5 Score: 29.261 bits (64), Expect = 2.382e-36
Identity = 15/49 (30.61%), Postives = 30/49 (61.22%), Query Frame = 2
Query:  416 IKLPKFMENNAVEQIEIIINYDIKTKFTHYFEDDLFLTRLSELHGYLMT 562
            I++P   + + ++ I + I Y    ++T  F+++LF+T LS++   LMT
Sbjct:  676 IRIPN--KKSKLDTIRLTITYYYDVEYTETFKENLFITELSQVTDQLMT 722          
The following BLAST results are available for this feature:
BLAST of SMED30028468 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028468 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028468 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028468 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028468 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028468 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028468 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028468 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028468 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028468 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028468 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028468 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028468 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028468 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000051017.16.435e-8662.11SMESG000051017.1[more]
SMESG000018599.12.089e-8561.05SMESG000018599.1[more]
SMESG000069427.12.926e-8461.58SMESG000069427.1[more]
SMESG000072420.13.285e-8461.58SMESG000072420.1[more]
SMESG000052400.11.180e-8361.58SMESG000052400.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30028468 ID=SMED30028468|Name=SMED30028468|organism=Schmidtea mediterranea sexual|type=transcript|length=1106bp
CCGATCTATGATTTTACCTAGTAATAGTTCTCTTATTTATCCTGATAATA
ATCCTAGTACTTTTGTTGTCGAATTTGAAAATCCAGTTTATTTGGATGGT
AAGTGGGAAGTGGCTTTGATGGACTTTACATTTGTGTATAATCATTTCCC
GTACTGTAGCAATTCATGTATACAATATAAATTCAAAAAATCGCACAAAG
AAACTAAATACTTGAAAATAAATAATGAAAATAGAATCTTAGAAATTTTA
AATTCTAAATATACTCCAGATGTTGATGTATTCATGGAGAATTATGAAAA
TTTATTGATTGTTTGTCCTCATGTTCCTTCTAAAATATCATTTCGCGATT
ACGAACATGCGTCTAAATTTGGATTACAAAACATATCCATCACTAGTGAA
TCTGATCATTGTATAATTAAATTACCTAAATTTATGGAAAATAATGCTGT
TGAACAAATTGAAATAATTATAAATTATGACATCAAGACTAAATTTACAC
ATTATTTTGAAGATGATTTATTCTTAACAAGATTATCAGAACTTCATGGA
TACTTAATGACAATCAATGCTTCCGTTTAAAGAAATCAATCTTAGAAATG
AAGAATCAGACATTTACTTATAAGGATCTTGTTGGTGTGAAAATTCCAAA
AATAATTCAAATTCATCAAATATTTAATTATTCAAATATTATTAAACCAA
TAAATGTTGGTAACGTAACAGTTCCTCTATTAAAATCTGTATGGATTAAA
AAACATGAACAGGGAGAGGTAGTACAGCTAGAAATGAAGAATCCAATCTA
CATCCGGATTTCATCTTCTTCTATAAACAACATTGAAGTTAATGTTCCTG
ACGATAGTGGAAAATTAATTAAATTTTCAAAAGATATCAAAACACATTTA
ACATTACATTTTCGAAAAATAAATGAATAATCCGGATATTGTAAACTTTT
ATAGAATTAGTCAATCTGGTGGAGATTTACCGTATTTTGTTAGTAAACAA
TATGGAACAGGATGGTTGAAAACAATAGGTCAATTTGCTTTACCAATTCT
TAAACGACTAAGGAATTTCGGTATGAAAACTACTAATGATGTAATAAATA
ACAATT
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protein sequence of SMED30028468-orf-1

>SMED30028468-orf-1 ID=SMED30028468-orf-1|Name=SMED30028468-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=111bp
MKNQTFTYKDLVGVKIPKIIQIHQIFNYSNIIKPINVGNVTVPLLKSVWI
KKHEQGEVVQLEMKNPIYIRISSSSINNIEVNVPDDSGKLIKFSKDIKTH
LTLHFRKINE*
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protein sequence of SMED30028468-orf-2

>SMED30028468-orf-2 ID=SMED30028468-orf-2|Name=SMED30028468-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=193bp
RSMILPSNSSLIYPDNNPSTFVVEFENPVYLDGKWEVALMDFTFVYNHFP
YCSNSCIQYKFKKSHKETKYLKINNENRILEILNSKYTPDVDVFMENYEN
LLIVCPHVPSKISFRDYEHASKFGLQNISITSESDHCIIKLPKFMENNAV
EQIEIIINYDIKTKFTHYFEDDLFLTRLSELHGYLMTINASV*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000074oocyte
PLANA:0000231vitelline gland