Lysosomal alpha-glucosidase
|
Overview
Neoblast Clusters
Zeng et. al., 2018
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny AppEmbryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Expression Details Lysosomal alpha-glucosidase is expressed during Stage 2, Stage 3, Stage 4, Stage 5 and Stage 6 in the embryonic digestive system, embryonic pharynx, embryonic gut cell and primitive gut cell
Click to see image symbols and abbreviations
Mitotic activity is restricted to piwi-1+ blastomeres, which cycle asynchronously. SPIM reconstructed S4 embryo constained with piwi-1 and LYAG-like (both in green) and H3S10p antibodies (red). LYAG-like marks the temporary embryonic pharynx and is not expressed in piwi-1+ blastomeres. Several examples of piwi-1+, H3S10p+ cells are evident. Anatomical Expression
PAGE et. al., 2020SMED30028442 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 12
Homology
BLAST of Lysosomal alpha-glucosidase vs. Ensembl Human
Match: GAA (glucosidase alpha, acid [Source:HGNC Symbol;Acc:HGNC:4065]) HSP 1 Score: 699.508 bits (1804), Expect = 0.000e+0 Identity = 359/872 (41.17%), Postives = 523/872 (59.98%), Query Frame = 3
Query: 321 QCQIANNNERLDCYPESGANQDSCIARGCCWSEPMRLSNKLQGVPLSIPYCYYPTDYSNYMIKSLRKSSRGIKAKLVRESPSYLPYEVAMLDLEVIYETKSRTRVRITDPKQKRYEVPMKLSPPDLKPPTDREYEVIIPSDGSFSIEIKRKSDDRSLF-KSFGPLLYSDQFLQISSIMSTDQIYGIGEQNRPFLHQLSNGWERIGLWARDNPIETFMNLYGAHPFYIGMPDDGKAFGVFHFNSNGQEFALQPAPAVTYRTIGGILDFFIFTGGSPEETVKQYLELVGKPMLPPYWSLGFHLSKWGYKNIEQLSEIFQRNINAGFPIGTQWSDIDYMENYKDFTVDKEKFNGLGRFVREIKQKYNSRFVAIVDPGISCTS-GNEYPPYYEGVFMDIFIKDSQTNQPIVGKVWPGETVYPDFTHPNISMYWYKQIQRFYNDVPIDGLWIDMNEPSNFYDGSKRGCQGNSKYDNPFFVPDIRDQSLISKTICSSALHYNNEKHYNLHNLYGYTESVTTNKALLKTIHGKRPFILSRSTFSGSGKYTFHWTGDNQASWDQLRNSIRQVLNFNMFGIPMTGADICGFNGNSDEELCIRWMQLGAFYPFMRNHNAINQRDQDPASWNTEAQKIMKTAISNRYAMLPLFYTLLVKSHMNGGTVMRPMSFEFFSDKVTHSIELQFMIGSSIMATPVVYPGARSVDAYFPAGVWYDFHS------GKL-----------LHSDAEGAWLTLDAPLDKINIALRGGHIVPLLDSNRNSKSNIPNKMTLYCGMAQDMSASGDLYWDDGESIDSYSRNRYSYVSFNASPEQL---LITPVYFGYDLLPVLNKIVILGFENKSITKFLLNDNDIP---FHYNPET 2861
QC + N+ R DC P+ Q+ C ARGCC+ + LQG + P+C++P Y +Y +++L S G A L R +P++ P ++ L L+V+ ET++R I DP +RYEVP++ + P+ Y V S+ F + ++R+ D R L + PL ++DQFLQ+S+ + + I G+ E P + LS W RI LW RD NLYG+HPFY+ + D G A GVF NSN + LQP+PA+++R+ GGILD +IF G P+ V+QYL++VG P +PPYW LGFHL +WGY + ++ + A FP+ QW+D+DYM++ +DFT +K+ F V+E+ Q R++ IVDP IS + Y PY EG+ +FI + +T QP++GKVWPG T +PDFT+P +W + F++ VP DG+WIDMNEPSNF GS+ GC N++ +NP +VP + +L + TIC+S+ H HYNLHNLYG TE++ +++AL+K G RPF++SRSTF+G G+Y HWTGD +SW+QL +S+ ++L FN+ G+P+ GAD+CGF GN+ EELC+RW QLGAFYPFMRNHN++ Q+P S++ AQ+ M+ A++ RYA+LP YTL ++H+ G TV RP+ EF D T +++ Q + G +++ TPV+ G V YFP G WYD + G L +HS EG W+TL APLD IN+ LR G+I+PL + + M L + + A G+L+WDDGES++ R Y+ V F A + L+ G L L K+ +LG N +P F Y+P+T
Sbjct: 81 QCDVPPNS-RFDCAPDKAITQEQCEARGCCY---IPAKQGLQGAQMGQPWCFFPPSYPSYKLENLSSSEMGYTATLTRTTPTFFPKDILTLRLDVMMETENRLHFTIKDPANRRYEVPLETPHVHSRAPSPL-YSVEF-SEEPFGVIVRRQLDGRVLLNTTVAPLFFADQFLQLSTSLPSQYITGLAEHLSPLM--LSTSWTRITLWNRDLAPTPGANLYGSHPFYLALEDGGSAHGVFLLNSNAMDVVLQPSPALSWRSTGGILDVYIFLGPEPKSVVQQYLDVVGYPFMPPYWGLGFHLCRWGYSSTAITRQVVENMTRAHFPLDVQWNDLDYMDSRRDFTFNKDGFRDFPAMVQELHQG-GRRYMMIVDPAISSSGPAGSYRPYDEGLRRGVFITN-ETGQPLIGKVWPGSTAFPDFTNPTALAWWEDMVAEFHDQVPFDGMWIDMNEPSNFIRGSEDGCP-NNELENPPYVPGVVGGTLQAATICASS-HQFLSTHYNLHNLYGLTEAIASHRALVKA-RGTRPFVISRSTFAGHGRYAGHWTGDVWSSWEQLASSVPEILQFNLLGVPLVGADVCGFLGNTSEELCVRWTQLGAFYPFMRNHNSLLSLPQEPYSFSEPAQQAMRKALTLRYALLPHLYTLFHQAHVAGETVARPLFLEFPKDSSTWTVDHQLLWGEALLITPVLQAGKAEVTGYFPLGTWYDLQTVPVEALGSLPPPPAAPREPAIHS--EGQWVTLPAPLDTINVHLRAGYIIPLQGPGLTTTESRQQPMALAVALTKGGEARGELFWDDGESLEVLERGAYTQVIFLARNNTIVNELVRVTSEGAGL--QLQKVTVLGVATAPQQVL---SNGVPVSNFTYSPDT 932
BLAST of Lysosomal alpha-glucosidase vs. Ensembl Human
Match: GAA (glucosidase alpha, acid [Source:HGNC Symbol;Acc:HGNC:4065]) HSP 1 Score: 699.508 bits (1804), Expect = 0.000e+0 Identity = 359/872 (41.17%), Postives = 523/872 (59.98%), Query Frame = 3
Query: 321 QCQIANNNERLDCYPESGANQDSCIARGCCWSEPMRLSNKLQGVPLSIPYCYYPTDYSNYMIKSLRKSSRGIKAKLVRESPSYLPYEVAMLDLEVIYETKSRTRVRITDPKQKRYEVPMKLSPPDLKPPTDREYEVIIPSDGSFSIEIKRKSDDRSLF-KSFGPLLYSDQFLQISSIMSTDQIYGIGEQNRPFLHQLSNGWERIGLWARDNPIETFMNLYGAHPFYIGMPDDGKAFGVFHFNSNGQEFALQPAPAVTYRTIGGILDFFIFTGGSPEETVKQYLELVGKPMLPPYWSLGFHLSKWGYKNIEQLSEIFQRNINAGFPIGTQWSDIDYMENYKDFTVDKEKFNGLGRFVREIKQKYNSRFVAIVDPGISCTS-GNEYPPYYEGVFMDIFIKDSQTNQPIVGKVWPGETVYPDFTHPNISMYWYKQIQRFYNDVPIDGLWIDMNEPSNFYDGSKRGCQGNSKYDNPFFVPDIRDQSLISKTICSSALHYNNEKHYNLHNLYGYTESVTTNKALLKTIHGKRPFILSRSTFSGSGKYTFHWTGDNQASWDQLRNSIRQVLNFNMFGIPMTGADICGFNGNSDEELCIRWMQLGAFYPFMRNHNAINQRDQDPASWNTEAQKIMKTAISNRYAMLPLFYTLLVKSHMNGGTVMRPMSFEFFSDKVTHSIELQFMIGSSIMATPVVYPGARSVDAYFPAGVWYDFHS------GKL-----------LHSDAEGAWLTLDAPLDKINIALRGGHIVPLLDSNRNSKSNIPNKMTLYCGMAQDMSASGDLYWDDGESIDSYSRNRYSYVSFNASPEQL---LITPVYFGYDLLPVLNKIVILGFENKSITKFLLNDNDIP---FHYNPET 2861
QC + N+ R DC P+ Q+ C ARGCC+ + LQG + P+C++P Y +Y +++L S G A L R +P++ P ++ L L+V+ ET++R I DP +RYEVP++ + P+ Y V S+ F + ++R+ D R L + PL ++DQFLQ+S+ + + I G+ E P + LS W RI LW RD NLYG+HPFY+ + D G A GVF NSN + LQP+PA+++R+ GGILD +IF G P+ V+QYL++VG P +PPYW LGFHL +WGY + ++ + A FP+ QW+D+DYM++ +DFT +K+ F V+E+ Q R++ IVDP IS + Y PY EG+ +FI + +T QP++GKVWPG T +PDFT+P +W + F++ VP DG+WIDMNEPSNF GS+ GC N++ +NP +VP + +L + TIC+S+ H HYNLHNLYG TE++ +++AL+K G RPF++SRSTF+G G+Y HWTGD +SW+QL +S+ ++L FN+ G+P+ GAD+CGF GN+ EELC+RW QLGAFYPFMRNHN++ Q+P S++ AQ+ M+ A++ RYA+LP YTL ++H+ G TV RP+ EF D T +++ Q + G +++ TPV+ G V YFP G WYD + G L +HS EG W+TL APLD IN+ LR G+I+PL + + M L + + A G+L+WDDGES++ R Y+ V F A + L+ G L L K+ +LG N +P F Y+P+T
Sbjct: 81 QCDVPPNS-RFDCAPDKAITQEQCEARGCCY---IPAKQGLQGAQMGQPWCFFPPSYPSYKLENLSSSEMGYTATLTRTTPTFFPKDILTLRLDVMMETENRLHFTIKDPANRRYEVPLETPHVHSRAPSPL-YSVEF-SEEPFGVIVRRQLDGRVLLNTTVAPLFFADQFLQLSTSLPSQYITGLAEHLSPLM--LSTSWTRITLWNRDLAPTPGANLYGSHPFYLALEDGGSAHGVFLLNSNAMDVVLQPSPALSWRSTGGILDVYIFLGPEPKSVVQQYLDVVGYPFMPPYWGLGFHLCRWGYSSTAITRQVVENMTRAHFPLDVQWNDLDYMDSRRDFTFNKDGFRDFPAMVQELHQG-GRRYMMIVDPAISSSGPAGSYRPYDEGLRRGVFITN-ETGQPLIGKVWPGSTAFPDFTNPTALAWWEDMVAEFHDQVPFDGMWIDMNEPSNFIRGSEDGCP-NNELENPPYVPGVVGGTLQAATICASS-HQFLSTHYNLHNLYGLTEAIASHRALVKA-RGTRPFVISRSTFAGHGRYAGHWTGDVWSSWEQLASSVPEILQFNLLGVPLVGADVCGFLGNTSEELCVRWTQLGAFYPFMRNHNSLLSLPQEPYSFSEPAQQAMRKALTLRYALLPHLYTLFHQAHVAGETVARPLFLEFPKDSSTWTVDHQLLWGEALLITPVLQAGKAEVTGYFPLGTWYDLQTVPVEALGSLPPPPAAPREPAIHS--EGQWVTLPAPLDTINVHLRAGYIIPLQGPGLTTTESRQQPMALAVALTKGGEARGELFWDDGESLEVLERGAYTQVIFLARNNTIVNELVRVTSEGAGL--QLQKVTVLGVATAPQQVL---SNGVPVSNFTYSPDT 932
BLAST of Lysosomal alpha-glucosidase vs. Ensembl Human
Match: MGAM (maltase-glucoamylase [Source:HGNC Symbol;Acc:HGNC:7043]) HSP 1 Score: 648.277 bits (1671), Expect = 0.000e+0 Identity = 352/861 (40.88%), Postives = 512/861 (59.47%), Query Frame = 3
Query: 312 ATAQCQIANNNERLDCYPESGANQDSCIARGCCWSEPMRLSNKLQGVPLSIPYCYYPTDYSNYMIKSLRKSSRGIKAKL--VRESPSYLPYEVAMLDLEVIYETKSRTRVRITDPKQKRYEVPMK-LSPPDLKPPTDREYEVIIPSDGSFSIEIKRKSDDRSLF-KSFGPLLYSDQFLQISSIMSTDQIYGIGEQ-NRPFLHQLSNGWERIGLWARDN-PIETFMNLYGAHPFYIGMPD-DGKAFGVFHFNSNGQEFALQPAPAVTYRTIGGILDFFIFTGGSPEETVKQYLELVGKPMLPPYWSLGFHLSKWGYKNIEQLSEIFQRNINAGFPIGTQWSDIDYMENYKDFTVDKEKFNGLGRFVREIKQKYNSRFVAIVDPGIS--CTSGNEYPPYYEGVFMDIFIKDSQTNQPIVGKVWPGETVYPDFTHPNISMYWYKQIQRFYNDVPIDGLWIDMNEPSNFYDGSKRGCQGNSKYDNPFFVPDIRDQSLISKTICSSALHYNNEKHYNLHNLYGYTESVTTNKALLKTIHGKRPFILSRSTFSGSGKYTFHWTGDNQASWDQLRNSIRQVLNFNMFGIPMTGADICGFNGNSDEELCIRWMQLGAFYPFMRNHNAINQRDQDPASWNTEAQKIMKTA--ISNRYAMLPLFYTLLVKSHMNGGTVMRPMSFEFFSDKVTHSIELQFMIGSSIMATPVVYPGARSVDAYFPAGVWYDFHSGKLLHSDAEGAWLTLDAPLDKINIALRGGHIVPLLDSNRNSKSNIPNKMTLYCGMAQDMSASGDLYWDDGESIDSYSRNRYSYVSFNASPEQLLITPVYFGYDLLPVL--NKIVILGFENKSITKFLLNDNDIPFHYNP 2855
+A+C + N ER++C P+ + +C RGCCW+ QG +S+P+CYY ++S ++ +L ++ G A+L + SP + V + L Y+T +R ++TD R+EVP + + Y+V I S FSI++ R+S++R LF S GPLL++DQFLQ+S+ + + +YG+GE ++ + H ++ W+ ++ RD P NLYGA F++ + D G +FGVF NSN E LQPAPA+TYRTIGGILDF++F G +PE+ V++YLEL+G+P LP YW+LGFHLS++ Y ++ + E+ +RN A P Q +DIDYM+ +DFT D F G FV E+ + V IVDP IS +S Y PY G M I++ S P++G+VWPG+TV+PD+T+PN +++W K+ + F+N V DG+WIDMNE SNF DGS GC N+ +NP F P I D L KT+C A+ + K Y++HNLYGY+ +V T +A KR FIL+RSTF+GSGK+ HW GDN A+WD LR SI VL FN+FGIPM G DICGF ++ EELC RWMQLGAFYPF RNHN +DQDPAS+ ++ + + ++ RY +LP YTL ++H G TV RP+ EF+ D T + QF+ G ++ TPV+ GA V AY P VWYD+ +G + + + ++ P DKI + LRGG+I P N + ++ N + L + ++ A G+L+WD+GE+ D+ + Y F+ + +L + Y L N+I ILG E S + N +P +P
Sbjct: 86 VSAECPVVNELERINCIPDQPPTKATCDQRGCCWNP--------QGA-VSVPWCYYSKNHSYHVEGNLVNTNAGFTARLKNLPSSPVF-GSNVDNVLLTAEYQTSNRFHFKLTDQTNNRFEVPHEHVQSFSGNAAASLTYQVEI-SRQPFSIKVTRRSNNRVLFDSSIGPLLFADQFLQLSTRLPSTNVYGLGEHVHQQYRHDMN--WKTWPIFNRDTTPNGNGTNLYGAQTFFLCLEDASGLSFGVFLMNSNAMEVVLQPAPAITYRTIGGILDFYVFLGNTPEQVVQEYLELIGRPALPSYWALGFHLSRYEYGTLDNMREVVERNRAAQLPYDVQHADIDYMDERRDFTYDSVDFKGFPEFVNELHNN-GQKLVIIVDPAISNNSSSSKPYGPYDRGSDMKIWVNSSDGVTPLIGEVWPGQTVFPDYTNPNCAVWWTKEFELFHNQVEFDGIWIDMNEVSNFVDGSVSGCSTNN-LNNPPFTPRILDGYLFCKTLCMDAVQHWG-KQYDIHNLYGYSMAVATAEAAKTVFPNKRSFILTRSTFAGSGKFAAHWLGDNTATWDDLRWSIPGVLEFNLFGIPMVGPDICGFALDTPEELCRRWMQLGAFYPFSRNHNGQGYKDQDPASFGADSLLLNSSRHYLNIRYTLLPYLYTLFFRAHSRGDTVARPLLHEFYEDNSTWDVHQQFLWGPGLLITPVLDEGAEKVMAYVPDAVWYDYETGSQVRWRKQK--VEMELPGDKIGLHLRGGYIFPTQQPNTTTLASRKNPLGLIIALDENKEAKGELFWDNGETKDTVANKVYLLCEFSVTQNRLEVNISQSTYKDPNNLAFNEIKILGTEEPS--NVTVKHNGVPSQTSP 926 HSP 2 Score: 600.897 bits (1548), Expect = 0.000e+0 Identity = 331/885 (37.40%), Postives = 507/885 (57.29%), Query Frame = 3
Query: 339 NNERLDCYP-ESGANQDSCIARGCCWSEPMRLSNKLQGVPLSIPYCYYPTDYSNYMIKSLRKSSRGIKAKLVRESPSYL----PYEVAMLDLEVIYETKSRTRVRITDPKQKRYEVPMKLSPPDL--KPPTDREYEVIIPSDGSFSIEIKRKSDDRSLFKS-FGPLLYSDQFLQISSIMSTDQIYGIGE-QNRPFLHQLSNGWERIGLWARDNPIETFMNLYGAHPFYIGMPDDGKAFGVFHFNSNGQEFALQPAPAVTYRTIGGILDFFIFTGGSPEETVKQYLELVGKPMLPPYWSLGFHLSKWGYKNIEQLSEIFQRNINAGFPIGTQWSDIDYMENYKDFTVDKEKFNGLGRFVREIKQKYNSRFVAIVDPGISCTSGNEYPPYYEGVFMDIFIKDSQTNQPIVGKVWP---------------------GETVYPDFTHPNISMYWYKQIQRFYND-------VPIDGLWIDMNEPSNFYDGSKR-GCQGNSKYDNPFFVPDI--RDQSLISKTICSSALHYNNE----KHYNLHNLYGYTESVTTNKALLKTIHGKRPFILSRSTFSGSGKYTFHWTGDNQASWDQLRNSIRQVLNFNMFGIPMTGADICGFNGNSDEELCIRWMQLGAFYPFMRNHNAINQRDQDPASWNTEAQKIMKTAISNRYAMLPLFYTLLVKSHMNGGTVMRPMSFEFFSDKVTHSIELQFMIGSSIMATPVVYPGARSVDAYFPAGVWYDFHSGKLLHSDAEGAWLTLDAPLDKINIALRGGHIVPLLDSNRNSKSNIPNKMTLYCGMAQDMSASGDLYWDDGESIDSYSRNRYSYVSFNASPEQLLITPVYFGYDLLPVL--NKIVILGFENKSITKFLLNDNDIPFHYNP 2855
+ E++DCYP E+GA+ ++C ARGC W ++ GVP +CY+ D Y + ++ +S G A + +S Y V L L+V Y + +I DP + RYEVP+ L+ P + P + Y+V+I + F IEI+RKS ++ S +SD F++IS+ + + +YG GE ++R + L W G+++RD P N YG HP+Y+G+ +DG A GV NSN + QP PA+TYRT GG+LDF++F G +PE +QY EL+G+P++ PYWSLGF L ++GY+N +++ ++ + A P Q+SDIDYME DFT+ KF G + +K R + I+DP IS YP + GV D+FIK + GKVWP +PDF + + +W ++I+ YN+ + DG+WIDMNEPS+F +G+ GC+ ++ ++P ++P + RD+ L SKT+C + + +HYN+HNLYG++++ T +A+ + + G+R +++RSTF SG++ HW GDN A+WDQL+ SI ++ F++FGI TGADICGF +++ E+C+RWMQLGAFYPF RNHN I R QDP SW+ I + + RY +LP YTL+ K+H G TV+RP+ EF SD+VT I+ QF++G + + +PV+ AR+V AYFP WYD+++G + +A G W TL APLD IN+ +RGG+I+P + N+ + N + L + ++ A G+L+WDDG++ D+ ++ Y F+ + L +T Y L N+I ILG E S + N +P +P
Sbjct: 960 DEEKIDCYPDENGASAENCTARGCIWE-----ASNSSGVP----FCYFVNDL--YSVSDVQYNSHGATADISLKSSVYANAFPSTPVNPLRLDVTYHKNEMLQFKIYDPNKNRYEVPVPLNIPSMPSSTPEGQLYDVLIKKN-PFGIEIRRKSTGTIIWDSQLLGFTFSDMFIRISTRLPSKYLYGFGETEHRSYRRDLE--WHTWGMFSRDQPPGYKKNSYGVHPYYMGLEEDGSAHGVLLLNSNAMDVTFQPLPALTYRTTGGVLDFYVFLGPTPELVTQQYTELIGRPVMVPYWSLGFQLCRYGYQNDSEIASLYDEMVAAQIPYDVQYSDIDYMERQLDFTL-SPKFAGFPALINRMKAD-GMRVILILDPAISGNETQPYPAFTRGVEDDVFIKYPNDGDIVWGKVWPDFPDVVVNGSLDWDSQVELYRAYVAFPDFFRNSTAKWWKREIEELYNNPQNPERSLKFDGMWIDMNEPSSFVNGAVSPGCR-DASLNHPPYMPHLESRDRGLSSKTLCMESQQILPDGSLVQHYNVHNLYGWSQTRPTYEAV-QEVTGQRGVVITRSTFPSSGRWAGHWLGDNTAAWDQLKKSIIGMMEFSLFGISYTGADICGFFQDAEYEMCVRWMQLGAFYPFSRNHNTIGTRRQDPVSWDAAFVNISRNVLQTRYTLLPYLYTLMQKAHTEGVTVVRPLLHEFVSDQVTWDIDSQFLLGPAFLVSPVLERNARNVTAYFPRARWYDYYTG--VDINARGEWKTLPAPLDHINLHVRGGYILPWQEPALNTHLSRKNPLGLIIALDENKEAKGELFWDDGQTKDTVAKKVYLLCEFSVTQNHLEVTISQSTYKDPNNLAFNEIKILGMEEPS--NVTVKHNGVPSQTSP 1822 HSP 3 Score: 591.652 bits (1524), Expect = 0.000e+0 Identity = 320/835 (38.32%), Postives = 487/835 (58.32%), Query Frame = 3
Query: 339 NNERLDCYP-ESGANQDSCIARGCCWSEPMRLSNKLQGVPLSIPYCYYPTDYSNYMIKSLRKSSRGIKAKLVRESP----SYLPYEVAMLDLEVIYETKSRTRVRITDPKQKRYEVPMKLSPPDL--KPPTDREYEVIIPSDGSFSIEIKRKSDDRSLFKS-FGPLLYSDQFLQISSIMSTDQIYGIGE-QNRPFLHQLSNGWERIGLWARDNPIETFMNLYGAHPFYIGMPDDGKAFGVFHFNSNGQEFALQPAPAVTYRTIGGILDFFIFTGGSPEETVKQYLELVGKPMLPPYWSLGFHLSKWGYKNIEQLSEIFQRNINAGFPIGTQWSDIDYMENYKDFTVDKEKFNGLGRFVREIKQKYNSRFVAIVDPGISCTSGNEYPPYYEGVFMDIFIKDSQTNQPIVGKVWP---------------------GETVYPDFTHPNISMYWYKQIQRFYND-------VPIDGLWIDMNEPSNFYDGSKR-GCQGNSKYDNPFFVP--DIRDQSLISKTICSSALHY----NNEKHYNLHNLYGYTESVTTNKALLKTIHGKRPFILSRSTFSGSGKYTFHWTGDNQASWDQLRNSIRQVLNFNMFGIPMTGADICGFNGNSDEELCIRWMQLGAFYPFMRNHNAINQRDQDPASWNTEAQKIMKTAISNRYAMLPLFYTLLVKSHMNGGTVMRPMSFEFFSDKVTHSIELQFMIGSSIMATPVVYPGARSVDAYFPAGVWYDFHSGKLLHSDAEGAWLTLDAPLDKINIALRGGHIVPLLDSNRNSKSNIPNKMTLYCGMAQDMSASGDLYWDDGESIDSYSRNRYSYVSFNAS 2711
+ E++DCYP E+G + ++C ARGC W ++ GVP +CY+ D Y + ++ +S G A + +S ++ V L L+V Y + +I DP RYEVP+ L+ P + P + Y+V+I + F IEI+RKS ++ S ++D F++IS+ + + +YG GE ++ + L W G+++RD P N YG HP+Y+G+ +DG A GV NSN + QP PA+TYRT GG+LDF++F G +PE +QY EL+G+P++ PYWSLGF L ++GY+N ++S ++ + A P Q+SDIDYME DFT+ KF G + +K R + I+DP IS YP + GV D+FIK + GKVWP +PDF + + +W ++I+ YN+ + DG+WIDMNEPS+F +G+ GC+ ++ ++P ++P + RD+ L SKT+C + + +HYN+HNLYG++++ T +A+ + + G+R +++RSTF SG++ HW GDN A+WDQL+ SI ++ F++FGI TGADICGF +++ E+C+RWMQLGAFYPF RNHN I R QDP SW+ I +T + RY +LP YTL+ K+H G TV+RP+ EF SD+VT I+ QF++G + + +PV+ AR+V AYFP WYD+++G + +A G W TL APLD IN+ +RGG+I+P + N+ + M + + +A G L+WDDG+SID+Y + Y SF+AS
Sbjct: 1856 DEEKIDCYPDENGDSAENCTARGCIWE-----ASNSSGVP----FCYFVNDL--YSVSDVQYNSHGATADISLKSSVHANAFPSTPVNPLRLDVTYHKNEMLQFKIYDPNNNRYEVPVPLNIPSVPSSTPEGQLYDVLIKKN-PFGIEIRRKSTGTIIWDSQLLGFTFNDMFIRISTRLPSKYLYGFGETEHTSYRRDLE--WHTWGMFSRDQPPGYKKNSYGVHPYYMGLEEDGSAHGVLLLNSNAMDVTFQPLPALTYRTTGGVLDFYVFLGPTPELVTQQYTELIGRPVMVPYWSLGFQLCRYGYQNDSEISSLYDEMVAAQIPYDVQYSDIDYMERQLDFTL-SPKFAGFPALINRMKAD-GMRVILILDPAISGNETQPYPAFTRGVEDDVFIKYPNDGDIVWGKVWPDFPDVVVNGSLDWDSQVELYRAYVAFPDFFRNSTAKWWKREIEELYNNPQNPERSLKFDGMWIDMNEPSSFVNGAVSPGCR-DASLNHPPYMPYLESRDRGLSSKTLCMESQQILPDGSPVQHYNVHNLYGWSQTRPTYEAV-QEVTGQRGVVITRSTFPSSGRWAGHWLGDNTAAWDQLKKSIIGMMEFSLFGISYTGADICGFFQDAEYEMCVRWMQLGAFYPFSRNHNTIGTRRQDPVSWDVAFVNISRTVLQTRYTLLPYLYTLMHKAHTEGVTVVRPLLHEFVSDQVTWDIDSQFLLGPAFLVSPVLERNARNVTAYFPRARWYDYYTG--VDINARGEWKTLPAPLDHINLHVRGGYILPWQEPALNTHLSRQKFMGFKIALDDEGTAGGWLFWDDGQSIDTYGKGLYYLASFSAS 2670
BLAST of Lysosomal alpha-glucosidase vs. Ensembl Human
Match: MGAM (maltase-glucoamylase [Source:HGNC Symbol;Acc:HGNC:7043]) HSP 1 Score: 647.892 bits (1670), Expect = 0.000e+0 Identity = 352/861 (40.88%), Postives = 512/861 (59.47%), Query Frame = 3
Query: 312 ATAQCQIANNNERLDCYPESGANQDSCIARGCCWSEPMRLSNKLQGVPLSIPYCYYPTDYSNYMIKSLRKSSRGIKAKL--VRESPSYLPYEVAMLDLEVIYETKSRTRVRITDPKQKRYEVPMK-LSPPDLKPPTDREYEVIIPSDGSFSIEIKRKSDDRSLF-KSFGPLLYSDQFLQISSIMSTDQIYGIGEQ-NRPFLHQLSNGWERIGLWARDN-PIETFMNLYGAHPFYIGMPD-DGKAFGVFHFNSNGQEFALQPAPAVTYRTIGGILDFFIFTGGSPEETVKQYLELVGKPMLPPYWSLGFHLSKWGYKNIEQLSEIFQRNINAGFPIGTQWSDIDYMENYKDFTVDKEKFNGLGRFVREIKQKYNSRFVAIVDPGIS--CTSGNEYPPYYEGVFMDIFIKDSQTNQPIVGKVWPGETVYPDFTHPNISMYWYKQIQRFYNDVPIDGLWIDMNEPSNFYDGSKRGCQGNSKYDNPFFVPDIRDQSLISKTICSSALHYNNEKHYNLHNLYGYTESVTTNKALLKTIHGKRPFILSRSTFSGSGKYTFHWTGDNQASWDQLRNSIRQVLNFNMFGIPMTGADICGFNGNSDEELCIRWMQLGAFYPFMRNHNAINQRDQDPASWNTEAQKIMKTA--ISNRYAMLPLFYTLLVKSHMNGGTVMRPMSFEFFSDKVTHSIELQFMIGSSIMATPVVYPGARSVDAYFPAGVWYDFHSGKLLHSDAEGAWLTLDAPLDKINIALRGGHIVPLLDSNRNSKSNIPNKMTLYCGMAQDMSASGDLYWDDGESIDSYSRNRYSYVSFNASPEQLLITPVYFGYDLLPVL--NKIVILGFENKSITKFLLNDNDIPFHYNP 2855
+A+C + N ER++C P+ + +C RGCCW+ QG +S+P+CYY ++S ++ +L ++ G A+L + SP + V + L Y+T +R ++TD R+EVP + + Y+V I S FSI++ R+S++R LF S GPLL++DQFLQ+S+ + + +YG+GE ++ + H ++ W+ ++ RD P NLYGA F++ + D G +FGVF NSN E LQPAPA+TYRTIGGILDF++F G +PE+ V++YLEL+G+P LP YW+LGFHLS++ Y ++ + E+ +RN A P Q +DIDYM+ +DFT D F G FV E+ + V IVDP IS +S Y PY G M I++ S P++G+VWPG+TV+PD+T+PN +++W K+ + F+N V DG+WIDMNE SNF DGS GC N+ +NP F P I D L KT+C A+ + K Y++HNLYGY+ +V T +A KR FIL+RSTF+GSGK+ HW GDN A+WD LR SI VL FN+FGIPM G DICGF ++ EELC RWMQLGAFYPF RNHN +DQDPAS+ ++ + + ++ RY +LP YTL ++H G TV RP+ EF+ D T + QF+ G ++ TPV+ GA V AY P VWYD+ +G + + + ++ P DKI + LRGG+I P N + ++ N + L + ++ A G+L+WD+GE+ D+ + Y F+ + +L + Y L N+I ILG E S + N +P +P
Sbjct: 86 VSAECPVVNELERINCIPDQPPTKATCDQRGCCWNP--------QGA-VSVPWCYYSKNHSYHVEGNLVNTNAGFTARLKNLPSSPVF-GSNVDNVLLTAEYQTSNRFHFKLTDQTNNRFEVPHEHVQSFSGNAAASLTYQVEI-SRQPFSIKVTRRSNNRVLFDSSIGPLLFADQFLQLSTRLPSTNVYGLGEHVHQQYRHDMN--WKTWPIFNRDTTPNGNGTNLYGAQTFFLCLEDASGLSFGVFLMNSNAMEVVLQPAPAITYRTIGGILDFYVFLGNTPEQVVQEYLELIGRPALPSYWALGFHLSRYEYGTLDNMREVVERNRAAQLPYDVQHADIDYMDERRDFTYDSVDFKGFPEFVNELHNN-GQKLVIIVDPAISNNSSSSKPYGPYDRGSDMKIWVNSSDGVTPLIGEVWPGQTVFPDYTNPNCAVWWTKEFELFHNQVEFDGIWIDMNEVSNFVDGSVSGCSTNN-LNNPPFTPRILDGYLFCKTLCMDAVQHWG-KQYDIHNLYGYSMAVATAEAAKTVFPNKRSFILTRSTFAGSGKFAAHWLGDNTATWDDLRWSIPGVLEFNLFGIPMVGPDICGFALDTPEELCRRWMQLGAFYPFSRNHNGQGYKDQDPASFGADSLLLNSSRHYLNIRYTLLPYLYTLFFRAHSRGDTVARPLLHEFYEDNSTWDVHQQFLWGPGLLITPVLDEGAEKVMAYVPDAVWYDYETGSQVRWRKQK--VEMELPGDKIGLHLRGGYIFPTQQPNTTTLASRKNPLGLIIALDENKEAKGELFWDNGETKDTVANKVYLLCEFSVTQNRLEVNISQSTYKDPNNLAFNEIKILGTEEPS--NVTVKHNGVPSQTSP 926 HSP 2 Score: 599.356 bits (1544), Expect = 0.000e+0 Identity = 323/835 (38.68%), Postives = 489/835 (58.56%), Query Frame = 3
Query: 339 NNERLDCYP-ESGANQDSCIARGCCWSEPMRLSNKLQGVPLSIPYCYYPTDYSNYMIKSLRKSSRGIKAKLVRESPSYL----PYEVAMLDLEVIYETKSRTRVRITDPKQKRYEVPMKLSPPDL--KPPTDREYEVIIPSDGSFSIEIKRKSDDRSLFKS-FGPLLYSDQFLQISSIMSTDQIYGIGE-QNRPFLHQLSNGWERIGLWARDNPIETFMNLYGAHPFYIGMPDDGKAFGVFHFNSNGQEFALQPAPAVTYRTIGGILDFFIFTGGSPEETVKQYLELVGKPMLPPYWSLGFHLSKWGYKNIEQLSEIFQRNINAGFPIGTQWSDIDYMENYKDFTVDKEKFNGLGRFVREIKQKYNSRFVAIVDPGISCTSGNEYPPYYEGVFMDIFIKDSQTNQPIVGKVWP---------------------GETVYPDFTHPNISMYWYKQIQRFYND-------VPIDGLWIDMNEPSNFYDGSKR-GCQGNSKYDNPFFVPDI--RDQSLISKTICSSALHYNNE----KHYNLHNLYGYTESVTTNKALLKTIHGKRPFILSRSTFSGSGKYTFHWTGDNQASWDQLRNSIRQVLNFNMFGIPMTGADICGFNGNSDEELCIRWMQLGAFYPFMRNHNAINQRDQDPASWNTEAQKIMKTAISNRYAMLPLFYTLLVKSHMNGGTVMRPMSFEFFSDKVTHSIELQFMIGSSIMATPVVYPGARSVDAYFPAGVWYDFHSGKLLHSDAEGAWLTLDAPLDKINIALRGGHIVPLLDSNRNSKSNIPNKMTLYCGMAQDMSASGDLYWDDGESIDSYSRNRYSYVSFNAS 2711
+ E++DCYP E+GA+ ++C ARGC W ++ GVP +CY+ D Y + ++ +S G A + +S Y V L L+V Y + +I DP + RYEVP+ L+ P + P + Y+V+I + F IEI+RKS ++ S +SD F++IS+ + + +YG GE ++R + L W G+++RD P N YG HP+Y+G+ +DG A GV NSN + QP PA+TYRT GG+LDF++F G +PE +QY EL+G+P++ PYWSLGF L ++GY+N +++ ++ + A P Q+SDIDYME DFT+ KF G + +K R + I+DP IS YP + GV D+FIK + GKVWP +PDF + + +W ++I+ YN+ + DG+WIDMNEPS+F +G+ GC+ ++ ++P ++P + RD+ L SKT+C + + +HYN+HNLYG++++ T +A+ + + G+R +++RSTF SG++ HW GDN A+WDQL+ SI ++ F++FGI TGADICGF +++ E+C+RWMQLGAFYPF RNHN I R QDP SW+ I +T + RY +LP YTL+ K+H G TV+RP+ EF SD+VT I+ QF++G + + +PV+ AR+V AYFP WYD+++G + +A G W TL APLD IN+ +RGG+I+P + N+ + M + + +A G L+WDDG+SID+Y + Y SF+AS
Sbjct: 960 DEEKIDCYPDENGASAENCTARGCIWE-----ASNSSGVP----FCYFVNDL--YSVSDVQYNSHGATADISLKSSVYANAFPSTPVNPLRLDVTYHKNEMLQFKIYDPNKNRYEVPVPLNIPSMPSSTPEGQLYDVLIKKN-PFGIEIRRKSTGTIIWDSQLLGFTFSDMFIRISTRLPSKYLYGFGETEHRSYRRDLE--WHTWGMFSRDQPPGYKKNSYGVHPYYMGLEEDGSAHGVLLLNSNAMDVTFQPLPALTYRTTGGVLDFYVFLGPTPELVTQQYTELIGRPVMVPYWSLGFQLCRYGYQNDSEIASLYDEMVAAQIPYDVQYSDIDYMERQLDFTL-SPKFAGFPALINRMKAD-GMRVILILDPAISGNETQPYPAFTRGVEDDVFIKYPNDGDIVWGKVWPDFPDVVVNGSLDWDSQVELYRAYVAFPDFFRNSTAKWWKREIEELYNNPQNPERSLKFDGMWIDMNEPSSFVNGAVSPGCR-DASLNHPPYMPHLESRDRGLSSKTLCMESQQILPDGSLVQHYNVHNLYGWSQTRPTYEAV-QEVTGQRGVVITRSTFPSSGRWAGHWLGDNTAAWDQLKKSIIGMMEFSLFGISYTGADICGFFQDAEYEMCVRWMQLGAFYPFSRNHNTIGTRRQDPVSWDVAFVNISRTVLQTRYTLLPYLYTLMHKAHTEGVTVVRPLLHEFVSDQVTWDIDSQFLLGPAFLVSPVLERNARNVTAYFPRARWYDYYTG--VDINARGEWKTLPAPLDHINLHVRGGYILPWQEPALNTHLSRQKFMGFKIALDDEGTAGGWLFWDDGQSIDTYGKGLYYLASFSAS 1774
BLAST of Lysosomal alpha-glucosidase vs. Ensembl Human
Match: MGAM (maltase-glucoamylase [Source:HGNC Symbol;Acc:HGNC:7043]) HSP 1 Score: 647.892 bits (1670), Expect = 0.000e+0 Identity = 352/861 (40.88%), Postives = 512/861 (59.47%), Query Frame = 3
Query: 312 ATAQCQIANNNERLDCYPESGANQDSCIARGCCWSEPMRLSNKLQGVPLSIPYCYYPTDYSNYMIKSLRKSSRGIKAKL--VRESPSYLPYEVAMLDLEVIYETKSRTRVRITDPKQKRYEVPMK-LSPPDLKPPTDREYEVIIPSDGSFSIEIKRKSDDRSLF-KSFGPLLYSDQFLQISSIMSTDQIYGIGEQ-NRPFLHQLSNGWERIGLWARDN-PIETFMNLYGAHPFYIGMPD-DGKAFGVFHFNSNGQEFALQPAPAVTYRTIGGILDFFIFTGGSPEETVKQYLELVGKPMLPPYWSLGFHLSKWGYKNIEQLSEIFQRNINAGFPIGTQWSDIDYMENYKDFTVDKEKFNGLGRFVREIKQKYNSRFVAIVDPGIS--CTSGNEYPPYYEGVFMDIFIKDSQTNQPIVGKVWPGETVYPDFTHPNISMYWYKQIQRFYNDVPIDGLWIDMNEPSNFYDGSKRGCQGNSKYDNPFFVPDIRDQSLISKTICSSALHYNNEKHYNLHNLYGYTESVTTNKALLKTIHGKRPFILSRSTFSGSGKYTFHWTGDNQASWDQLRNSIRQVLNFNMFGIPMTGADICGFNGNSDEELCIRWMQLGAFYPFMRNHNAINQRDQDPASWNTEAQKIMKTA--ISNRYAMLPLFYTLLVKSHMNGGTVMRPMSFEFFSDKVTHSIELQFMIGSSIMATPVVYPGARSVDAYFPAGVWYDFHSGKLLHSDAEGAWLTLDAPLDKINIALRGGHIVPLLDSNRNSKSNIPNKMTLYCGMAQDMSASGDLYWDDGESIDSYSRNRYSYVSFNASPEQLLITPVYFGYDLLPVL--NKIVILGFENKSITKFLLNDNDIPFHYNP 2855
+A+C + N ER++C P+ + +C RGCCW+ QG +S+P+CYY ++S ++ +L ++ G A+L + SP + V + L Y+T +R ++TD R+EVP + + Y+V I S FSI++ R+S++R LF S GPLL++DQFLQ+S+ + + +YG+GE ++ + H ++ W+ ++ RD P NLYGA F++ + D G +FGVF NSN E LQPAPA+TYRTIGGILDF++F G +PE+ V++YLEL+G+P LP YW+LGFHLS++ Y ++ + E+ +RN A P Q +DIDYM+ +DFT D F G FV E+ + V IVDP IS +S Y PY G M I++ S P++G+VWPG+TV+PD+T+PN +++W K+ + F+N V DG+WIDMNE SNF DGS GC N+ +NP F P I D L KT+C A+ + K Y++HNLYGY+ +V T +A KR FIL+RSTF+GSGK+ HW GDN A+WD LR SI VL FN+FGIPM G DICGF ++ EELC RWMQLGAFYPF RNHN +DQDPAS+ ++ + + ++ RY +LP YTL ++H G TV RP+ EF+ D T + QF+ G ++ TPV+ GA V AY P VWYD+ +G + + + ++ P DKI + LRGG+I P N + ++ N + L + ++ A G+L+WD+GE+ D+ + Y F+ + +L + Y L N+I ILG E S + N +P +P
Sbjct: 86 VSAECPVVNELERINCIPDQPPTKATCDQRGCCWNP--------QGA-VSVPWCYYSKNHSYHVEGNLVNTNAGFTARLKNLPSSPVF-GSNVDNVLLTAEYQTSNRFHFKLTDQTNNRFEVPHEHVQSFSGNAAASLTYQVEI-SRQPFSIKVTRRSNNRVLFDSSIGPLLFADQFLQLSTRLPSTNVYGLGEHVHQQYRHDMN--WKTWPIFNRDTTPNGNGTNLYGAQTFFLCLEDASGLSFGVFLMNSNAMEVVLQPAPAITYRTIGGILDFYVFLGNTPEQVVQEYLELIGRPALPSYWALGFHLSRYEYGTLDNMREVVERNRAAQLPYDVQHADIDYMDERRDFTYDSVDFKGFPEFVNELHNN-GQKLVIIVDPAISNNSSSSKPYGPYDRGSDMKIWVNSSDGVTPLIGEVWPGQTVFPDYTNPNCAVWWTKEFELFHNQVEFDGIWIDMNEVSNFVDGSVSGCSTNN-LNNPPFTPRILDGYLFCKTLCMDAVQHWG-KQYDIHNLYGYSMAVATAEAAKTVFPNKRSFILTRSTFAGSGKFAAHWLGDNTATWDDLRWSIPGVLEFNLFGIPMVGPDICGFALDTPEELCRRWMQLGAFYPFSRNHNGQGYKDQDPASFGADSLLLNSSRHYLNIRYTLLPYLYTLFFRAHSRGDTVARPLLHEFYEDNSTWDVHQQFLWGPGLLITPVLDEGAEKVMAYVPDAVWYDYETGSQVRWRKQK--VEMELPGDKIGLHLRGGYIFPTQQPNTTTLASRKNPLGLIIALDENKEAKGELFWDNGETKDTVANKVYLLCEFSVTQNRLEVNISQSTYKDPNNLAFNEIKILGTEEPS--NVTVKHNGVPSQTSP 926 HSP 2 Score: 599.356 bits (1544), Expect = 0.000e+0 Identity = 323/835 (38.68%), Postives = 489/835 (58.56%), Query Frame = 3
Query: 339 NNERLDCYP-ESGANQDSCIARGCCWSEPMRLSNKLQGVPLSIPYCYYPTDYSNYMIKSLRKSSRGIKAKLVRESPSYL----PYEVAMLDLEVIYETKSRTRVRITDPKQKRYEVPMKLSPPDL--KPPTDREYEVIIPSDGSFSIEIKRKSDDRSLFKS-FGPLLYSDQFLQISSIMSTDQIYGIGE-QNRPFLHQLSNGWERIGLWARDNPIETFMNLYGAHPFYIGMPDDGKAFGVFHFNSNGQEFALQPAPAVTYRTIGGILDFFIFTGGSPEETVKQYLELVGKPMLPPYWSLGFHLSKWGYKNIEQLSEIFQRNINAGFPIGTQWSDIDYMENYKDFTVDKEKFNGLGRFVREIKQKYNSRFVAIVDPGISCTSGNEYPPYYEGVFMDIFIKDSQTNQPIVGKVWP---------------------GETVYPDFTHPNISMYWYKQIQRFYND-------VPIDGLWIDMNEPSNFYDGSKR-GCQGNSKYDNPFFVPDI--RDQSLISKTICSSALHYNNE----KHYNLHNLYGYTESVTTNKALLKTIHGKRPFILSRSTFSGSGKYTFHWTGDNQASWDQLRNSIRQVLNFNMFGIPMTGADICGFNGNSDEELCIRWMQLGAFYPFMRNHNAINQRDQDPASWNTEAQKIMKTAISNRYAMLPLFYTLLVKSHMNGGTVMRPMSFEFFSDKVTHSIELQFMIGSSIMATPVVYPGARSVDAYFPAGVWYDFHSGKLLHSDAEGAWLTLDAPLDKINIALRGGHIVPLLDSNRNSKSNIPNKMTLYCGMAQDMSASGDLYWDDGESIDSYSRNRYSYVSFNAS 2711
+ E++DCYP E+GA+ ++C ARGC W ++ GVP +CY+ D Y + ++ +S G A + +S Y V L L+V Y + +I DP + RYEVP+ L+ P + P + Y+V+I + F IEI+RKS ++ S +SD F++IS+ + + +YG GE ++R + L W G+++RD P N YG HP+Y+G+ +DG A GV NSN + QP PA+TYRT GG+LDF++F G +PE +QY EL+G+P++ PYWSLGF L ++GY+N +++ ++ + A P Q+SDIDYME DFT+ KF G + +K R + I+DP IS YP + GV D+FIK + GKVWP +PDF + + +W ++I+ YN+ + DG+WIDMNEPS+F +G+ GC+ ++ ++P ++P + RD+ L SKT+C + + +HYN+HNLYG++++ T +A+ + + G+R +++RSTF SG++ HW GDN A+WDQL+ SI ++ F++FGI TGADICGF +++ E+C+RWMQLGAFYPF RNHN I R QDP SW+ I +T + RY +LP YTL+ K+H G TV+RP+ EF SD+VT I+ QF++G + + +PV+ AR+V AYFP WYD+++G + +A G W TL APLD IN+ +RGG+I+P + N+ + M + + +A G L+WDDG+SID+Y + Y SF+AS
Sbjct: 960 DEEKIDCYPDENGASAENCTARGCIWE-----ASNSSGVP----FCYFVNDL--YSVSDVQYNSHGATADISLKSSVYANAFPSTPVNPLRLDVTYHKNEMLQFKIYDPNKNRYEVPVPLNIPSMPSSTPEGQLYDVLIKKN-PFGIEIRRKSTGTIIWDSQLLGFTFSDMFIRISTRLPSKYLYGFGETEHRSYRRDLE--WHTWGMFSRDQPPGYKKNSYGVHPYYMGLEEDGSAHGVLLLNSNAMDVTFQPLPALTYRTTGGVLDFYVFLGPTPELVTQQYTELIGRPVMVPYWSLGFQLCRYGYQNDSEIASLYDEMVAAQIPYDVQYSDIDYMERQLDFTL-SPKFAGFPALINRMKAD-GMRVILILDPAISGNETQPYPAFTRGVEDDVFIKYPNDGDIVWGKVWPDFPDVVVNGSLDWDSQVELYRAYVAFPDFFRNSTAKWWKREIEELYNNPQNPERSLKFDGMWIDMNEPSSFVNGAVSPGCR-DASLNHPPYMPHLESRDRGLSSKTLCMESQQILPDGSLVQHYNVHNLYGWSQTRPTYEAV-QEVTGQRGVVITRSTFPSSGRWAGHWLGDNTAAWDQLKKSIIGMMEFSLFGISYTGADICGFFQDAEYEMCVRWMQLGAFYPFSRNHNTIGTRRQDPVSWDVAFVNISRTVLQTRYTLLPYLYTLMHKAHTEGVTVVRPLLHEFVSDQVTWDIDSQFLLGPAFLVSPVLERNARNVTAYFPRARWYDYYTG--VDINARGEWKTLPAPLDHINLHVRGGYILPWQEPALNTHLSRQKFMGFKIALDDEGTAGGWLFWDDGQSIDTYGKGLYYLASFSAS 1774
BLAST of Lysosomal alpha-glucosidase vs. Ensembl Celegans
Match: aagr-1 (Acid Alpha Glucosidase Relate [Source:UniProtKB/TrEMBL;Acc:Q19004]) HSP 1 Score: 483.411 bits (1243), Expect = 2.216e-155 Identity = 292/830 (35.18%), Postives = 438/830 (52.77%), Query Frame = 3
Query: 327 QIANNNERLDCYPESGANQDSCIARGCCWSEPMRLSNKLQGVPLSIPYCYYPTDYSNYMIKSLRKSSRGIKAKLVRESPSYLPYEVAMLDLEVIYETKSRTRVRITDPKQKRYEVPMKLSPPDLKPPTDRE----YEVIIPSDGSFSIEIKRKSDDRSLF-KSFGPLLYSDQFLQISSIMSTDQIYGIGEQNRPFLHQLSNGWERIGLWARD-----NPIETFMNLYGAHPFYIGMPDDGKAFGVFHFNSNGQEFALQPAPAVTYRTIGGILDFFIFTGGSPEETVKQYLELVGKPMLPPYWSLGFHLSKWGYKNIEQLSEIFQRNINAGFPIGTQWSDIDYMENYKDFTVDKEKFNGLGRFVREIKQKYNSRFVAIVDPGISCTSGNEYPPYYEGVFMDI-FIKDSQTNQP----------------IVGKVWPGE-TVYPDFTHP--NISMYWYKQIQRFYNDVPIDGLWIDMNEPSNFYDGS-----------KRGCQGNSKYDNPFFVPDIRDQS--------LISKTICS-SALHYNNEKHYNLHNLYGYTESVTTNKALLKTIHGKRPFILSRSTFSGSGKYTFHWTGDNQASWDQLRNSIRQVLNFNMFGIPMTGADICGFNGNSDEELCIRWMQLGAFYPFMRNHNAINQRDQDPASWNTEAQKIMKTAISNRYAMLPLFYTLLVKSHMNGGTVMRPMSFEFFSDKVTHSIELQFMIGSSIMATPVVYPGARSVDAYFPAGVWYDFHSGKLLHSDAEGAWLTLDAPLDKIN-IALRGGHIVPLLDSNRNSKSNIPNKMTLYCGMAQDMSASGDLYWDDGESI 2663
Q + ++R+DCYPE GA+QD+C +RGC W+E S P+ P+CYYPT+ S + ++S +S + AK PY + L V Y + T + +T RY P+ KP T E I S FS ++ R S +L+ S G + ++D+F+QI++ + + IYG G+ + + + ++ARD + NLYG HPFY+ + DGKA GVF NSN QE P P + YRTIGG +D F G +PE+ V QYL+ +G P LP YW+LG+ L +WGY N++ + + RN G P+ ++DIDYM +Y+DFT + + ++G + ++I + + I DP + +Y + G+ D FI+ ++ +Q ++G VWP T +PDF P N + +W + +F+ +P DG+WIDMNEPSNF G+ K C D+ VP Q+ L SKT+C + Y+ NLYG++E+ T +A+ + + GKR ++SRSTF SG+Y HW GDN A W L+ S+ V+ FNMFG+P G+DICGFNG S+EELC+RW Q GAF PF R+HN+ DQDPA W + A K A++ RY LP Y+L + G TV+RP+ FEF D+ T +I QF+ GS++M P +Y G SV AYFP+ WY G + ++APL + + +RGG ++P + ++ + L + + ++SGDLY+D G+ +
Sbjct: 23 QSVDVSKRVDCYPEPGASQDACQSRGCIWTEAPSSS------PVGTPWCYYPTE-SGFTVQSTGTNSFVLAAKTKN------PYGNNISPLNVKYSSNGATLL-LTIGNDDRYVPPVNFPK---KPSTSTESLKFTSGTIGSSDVFSFKVTRASTGVALWDTSIGGMQFADKFIQIATYLPSKNIYGFGDHIHKKIRHNLDRYTTWPMFARDIGPDSGSALSTQNLYGVHPFYMCIEADGKAHGVFILNSNAQEVETGPGPHLLYRTIGGRIDMAFFPGPTPEQVVNQYLQHIGFPFLPAYWALGYQLCRWGYGNLDAMKTVISRNQALGIPLDVPYADIDYMNHYEDFT-EGDNWSGFPAYTQQIHAQ-GLHLIVIFDPAVEV----DYASFQRGINADASFIEWARDDQVPHNIQDQYPMAKNTKIMLGNVWPDRNTAFPDFLDPRNNTNAWWAGEFAQFHKTLPFDGMWIDMNEPSNFDTGTYNTVEEQLASAKLSCPITGS-DSSLDVPPYPTQAVYQRNGEYLFSKTLCMLGKTAHRTRDFYDTKNLYGWSEARATYQAIPQ-VTGKRSAVISRSTFPSSGRYGGHWLGDNTARWGDLQTSVIGVMEFNMFGVPYVGSDICGFNGVSNEELCLRWHQFGAFSPFSRDHNSEGMPDQDPAVWPSVANA-AKIALTFRYYFLPFLYSLHYNAARYGHTVIRPLFFEFPKDEETLNISEQFLWGSALMIAPALYQGQTSVHAYFPSDTWYSLQPETYGQKMFSG-FNDVNAPLSSLTPVFVRGGFVLPRQSPGTTTTASRLSPFELLITVKTNAASSGDLYYDGGDDL 825
BLAST of Lysosomal alpha-glucosidase vs. Ensembl Celegans
Match: aagr-1 (Acid Alpha Glucosidase Relate [Source:UniProtKB/TrEMBL;Acc:Q19004]) HSP 1 Score: 483.411 bits (1243), Expect = 2.216e-155 Identity = 292/830 (35.18%), Postives = 438/830 (52.77%), Query Frame = 3
Query: 327 QIANNNERLDCYPESGANQDSCIARGCCWSEPMRLSNKLQGVPLSIPYCYYPTDYSNYMIKSLRKSSRGIKAKLVRESPSYLPYEVAMLDLEVIYETKSRTRVRITDPKQKRYEVPMKLSPPDLKPPTDRE----YEVIIPSDGSFSIEIKRKSDDRSLF-KSFGPLLYSDQFLQISSIMSTDQIYGIGEQNRPFLHQLSNGWERIGLWARD-----NPIETFMNLYGAHPFYIGMPDDGKAFGVFHFNSNGQEFALQPAPAVTYRTIGGILDFFIFTGGSPEETVKQYLELVGKPMLPPYWSLGFHLSKWGYKNIEQLSEIFQRNINAGFPIGTQWSDIDYMENYKDFTVDKEKFNGLGRFVREIKQKYNSRFVAIVDPGISCTSGNEYPPYYEGVFMDI-FIKDSQTNQP----------------IVGKVWPGE-TVYPDFTHP--NISMYWYKQIQRFYNDVPIDGLWIDMNEPSNFYDGS-----------KRGCQGNSKYDNPFFVPDIRDQS--------LISKTICS-SALHYNNEKHYNLHNLYGYTESVTTNKALLKTIHGKRPFILSRSTFSGSGKYTFHWTGDNQASWDQLRNSIRQVLNFNMFGIPMTGADICGFNGNSDEELCIRWMQLGAFYPFMRNHNAINQRDQDPASWNTEAQKIMKTAISNRYAMLPLFYTLLVKSHMNGGTVMRPMSFEFFSDKVTHSIELQFMIGSSIMATPVVYPGARSVDAYFPAGVWYDFHSGKLLHSDAEGAWLTLDAPLDKIN-IALRGGHIVPLLDSNRNSKSNIPNKMTLYCGMAQDMSASGDLYWDDGESI 2663
Q + ++R+DCYPE GA+QD+C +RGC W+E S P+ P+CYYPT+ S + ++S +S + AK PY + L V Y + T + +T RY P+ KP T E I S FS ++ R S +L+ S G + ++D+F+QI++ + + IYG G+ + + + ++ARD + NLYG HPFY+ + DGKA GVF NSN QE P P + YRTIGG +D F G +PE+ V QYL+ +G P LP YW+LG+ L +WGY N++ + + RN G P+ ++DIDYM +Y+DFT + + ++G + ++I + + I DP + +Y + G+ D FI+ ++ +Q ++G VWP T +PDF P N + +W + +F+ +P DG+WIDMNEPSNF G+ K C D+ VP Q+ L SKT+C + Y+ NLYG++E+ T +A+ + + GKR ++SRSTF SG+Y HW GDN A W L+ S+ V+ FNMFG+P G+DICGFNG S+EELC+RW Q GAF PF R+HN+ DQDPA W + A K A++ RY LP Y+L + G TV+RP+ FEF D+ T +I QF+ GS++M P +Y G SV AYFP+ WY G + ++APL + + +RGG ++P + ++ + L + + ++SGDLY+D G+ +
Sbjct: 23 QSVDVSKRVDCYPEPGASQDACQSRGCIWTEAPSSS------PVGTPWCYYPTE-SGFTVQSTGTNSFVLAAKTKN------PYGNNISPLNVKYSSNGATLL-LTIGNDDRYVPPVNFPK---KPSTSTESLKFTSGTIGSSDVFSFKVTRASTGVALWDTSIGGMQFADKFIQIATYLPSKNIYGFGDHIHKKIRHNLDRYTTWPMFARDIGPDSGSALSTQNLYGVHPFYMCIEADGKAHGVFILNSNAQEVETGPGPHLLYRTIGGRIDMAFFPGPTPEQVVNQYLQHIGFPFLPAYWALGYQLCRWGYGNLDAMKTVISRNQALGIPLDVPYADIDYMNHYEDFT-EGDNWSGFPAYTQQIHAQ-GLHLIVIFDPAVEV----DYASFQRGINADASFIEWARDDQVPHNIQDQYPMAKNTKIMLGNVWPDRNTAFPDFLDPRNNTNAWWAGEFAQFHKTLPFDGMWIDMNEPSNFDTGTYNTVEEQLASAKLSCPITGS-DSSLDVPPYPTQAVYQRNGEYLFSKTLCMLGKTAHRTRDFYDTKNLYGWSEARATYQAIPQ-VTGKRSAVISRSTFPSSGRYGGHWLGDNTARWGDLQTSVIGVMEFNMFGVPYVGSDICGFNGVSNEELCLRWHQFGAFSPFSRDHNSEGMPDQDPAVWPSVANA-AKIALTFRYYFLPFLYSLHYNAARYGHTVIRPLFFEFPKDEETLNISEQFLWGSALMIAPALYQGQTSVHAYFPSDTWYSLQPETYGQKMFSG-FNDVNAPLSSLTPVFVRGGFVLPRQSPGTTTTASRLSPFELLITVKTNAASSGDLYYDGGDDL 825
BLAST of Lysosomal alpha-glucosidase vs. Ensembl Celegans
Match: aagr-1 (Acid Alpha Glucosidase Relate [Source:UniProtKB/TrEMBL;Acc:Q19004]) HSP 1 Score: 483.411 bits (1243), Expect = 2.216e-155 Identity = 292/830 (35.18%), Postives = 438/830 (52.77%), Query Frame = 3
Query: 327 QIANNNERLDCYPESGANQDSCIARGCCWSEPMRLSNKLQGVPLSIPYCYYPTDYSNYMIKSLRKSSRGIKAKLVRESPSYLPYEVAMLDLEVIYETKSRTRVRITDPKQKRYEVPMKLSPPDLKPPTDRE----YEVIIPSDGSFSIEIKRKSDDRSLF-KSFGPLLYSDQFLQISSIMSTDQIYGIGEQNRPFLHQLSNGWERIGLWARD-----NPIETFMNLYGAHPFYIGMPDDGKAFGVFHFNSNGQEFALQPAPAVTYRTIGGILDFFIFTGGSPEETVKQYLELVGKPMLPPYWSLGFHLSKWGYKNIEQLSEIFQRNINAGFPIGTQWSDIDYMENYKDFTVDKEKFNGLGRFVREIKQKYNSRFVAIVDPGISCTSGNEYPPYYEGVFMDI-FIKDSQTNQP----------------IVGKVWPGE-TVYPDFTHP--NISMYWYKQIQRFYNDVPIDGLWIDMNEPSNFYDGS-----------KRGCQGNSKYDNPFFVPDIRDQS--------LISKTICS-SALHYNNEKHYNLHNLYGYTESVTTNKALLKTIHGKRPFILSRSTFSGSGKYTFHWTGDNQASWDQLRNSIRQVLNFNMFGIPMTGADICGFNGNSDEELCIRWMQLGAFYPFMRNHNAINQRDQDPASWNTEAQKIMKTAISNRYAMLPLFYTLLVKSHMNGGTVMRPMSFEFFSDKVTHSIELQFMIGSSIMATPVVYPGARSVDAYFPAGVWYDFHSGKLLHSDAEGAWLTLDAPLDKIN-IALRGGHIVPLLDSNRNSKSNIPNKMTLYCGMAQDMSASGDLYWDDGESI 2663
Q + ++R+DCYPE GA+QD+C +RGC W+E S P+ P+CYYPT+ S + ++S +S + AK PY + L V Y + T + +T RY P+ KP T E I S FS ++ R S +L+ S G + ++D+F+QI++ + + IYG G+ + + + ++ARD + NLYG HPFY+ + DGKA GVF NSN QE P P + YRTIGG +D F G +PE+ V QYL+ +G P LP YW+LG+ L +WGY N++ + + RN G P+ ++DIDYM +Y+DFT + + ++G + ++I + + I DP + +Y + G+ D FI+ ++ +Q ++G VWP T +PDF P N + +W + +F+ +P DG+WIDMNEPSNF G+ K C D+ VP Q+ L SKT+C + Y+ NLYG++E+ T +A+ + + GKR ++SRSTF SG+Y HW GDN A W L+ S+ V+ FNMFG+P G+DICGFNG S+EELC+RW Q GAF PF R+HN+ DQDPA W + A K A++ RY LP Y+L + G TV+RP+ FEF D+ T +I QF+ GS++M P +Y G SV AYFP+ WY G + ++APL + + +RGG ++P + ++ + L + + ++SGDLY+D G+ +
Sbjct: 23 QSVDVSKRVDCYPEPGASQDACQSRGCIWTEAPSSS------PVGTPWCYYPTE-SGFTVQSTGTNSFVLAAKTKN------PYGNNISPLNVKYSSNGATLL-LTIGNDDRYVPPVNFPK---KPSTSTESLKFTSGTIGSSDVFSFKVTRASTGVALWDTSIGGMQFADKFIQIATYLPSKNIYGFGDHIHKKIRHNLDRYTTWPMFARDIGPDSGSALSTQNLYGVHPFYMCIEADGKAHGVFILNSNAQEVETGPGPHLLYRTIGGRIDMAFFPGPTPEQVVNQYLQHIGFPFLPAYWALGYQLCRWGYGNLDAMKTVISRNQALGIPLDVPYADIDYMNHYEDFT-EGDNWSGFPAYTQQIHAQ-GLHLIVIFDPAVEV----DYASFQRGINADASFIEWARDDQVPHNIQDQYPMAKNTKIMLGNVWPDRNTAFPDFLDPRNNTNAWWAGEFAQFHKTLPFDGMWIDMNEPSNFDTGTYNTVEEQLASAKLSCPITGS-DSSLDVPPYPTQAVYQRNGEYLFSKTLCMLGKTAHRTRDFYDTKNLYGWSEARATYQAIPQ-VTGKRSAVISRSTFPSSGRYGGHWLGDNTARWGDLQTSVIGVMEFNMFGVPYVGSDICGFNGVSNEELCLRWHQFGAFSPFSRDHNSEGMPDQDPAVWPSVANA-AKIALTFRYYFLPFLYSLHYNAARYGHTVIRPLFFEFPKDEETLNISEQFLWGSALMIAPALYQGQTSVHAYFPSDTWYSLQPETYGQKMFSG-FNDVNAPLSSLTPVFVRGGFVLPRQSPGTTTTASRLSPFELLITVKTNAASSGDLYYDGGDDL 825
BLAST of Lysosomal alpha-glucosidase vs. Ensembl Celegans
Match: aagr-2 (Acid Alpha Glucosidase Relate [Source:UniProtKB/TrEMBL;Acc:Q21750]) HSP 1 Score: 460.299 bits (1183), Expect = 2.372e-146 Identity = 290/846 (34.28%), Postives = 421/846 (49.76%), Query Frame = 3
Query: 336 NNNERLDCYPESGANQDSCIARGCCWSEPMRLSNKLQGVPLSIPYCYYPTDYSNYMIKSLRKSSRGIKAKLVRESPSYLPYEVAMLDLEVIYETKSRTRV-RITDPKQKRYEVPMKLSPPDLKPPTDREYEVIIPSDGSFSIEIKRKSDDRSLF-KSFGPLLYSDQFLQISSIMSTDQIYGIGEQNRPFLHQLSNGWERIGLWARDNPIET----FMNLYGAHPFYIGMPDDGKAFGVFHFNSNGQEFALQPAPAVTYRTIGGILDFFIFTGGSPEETVKQYLELVGKPMLPPYWSLGFHLSKWGYKNIEQLSEIFQRNINAGFPIGTQWSDIDYMENYKDFTVDKEKFNGLGRFVREIKQKYNSRFVAIVDPGISCTSGNEYPPYYEGVFMDIFIKDSQTNQPI-----------------VGKVWPGETV-YPDF--THPNISMYWYKQIQRFYNDVPIDGLWIDMNEPSNFYDGSKRGCQGNSK---YDNPFFVP---------------------DIRDQSLISK-TICSSALH-YNNEKHYNLHNLYGYTESVTTNKALLKTIHGKRPFILSRSTFSGSGKYTFHWTGDNQASWDQLRNSIRQVLNFNMFGIPMTGADICGFNGNSDEELCIRWMQLGAFYPFMRNHNAINQRDQDPASWNTEAQKIMKTAISNRYAMLPLFYTLLVKSHMNGGTVMRPMSFEFFSDKVTHSIELQFMIGSSIMATPVVYPGARSVDAYFPAGVWY---DFHSGKLLHSDAEGAWLTLDAP-LDKINIALRGGHIVPLLDSNRNSKSNIPNKMTLYCGMAQDMSASGDLYWDDGESIDSYSRNRYSYVSFN 2705
N R DC PE NQ +C ARGC W E N P+CY+ D Y + S + S+ ++ +P E+ TKS V + RYE P+ P + + T+ S+G FS + R+S +R LF S G L++SDQF+QI++ + ++ +YG GE L + ++ARD P + +NLYG HP+Y+ + DGKA GV NSN QE P P++ YRTIGG LD + F G +PE +QYL+ +GKP LP YW+LG+ LS++GYK + ++ Q +AG PI +DIDYM+ YKDFT + + G +V+ + + + + I DP I T YP + + + + +T + +G VWP V +PDF + N +W + + + V DG+WIDMNEPSNF +S D P F P D + +S T+C A ++ YN+ NLYG TE++ T KAL K GKR ++SRST+ +G+Y HW GDN A W+ LR S+ FN+FGIP G+D+CGF G + EELC+RW Q+GAF+ F RNHN I QDPA W + A K + RY LP ++L + ++G TV+RP+ FE+ +D T ++ +FM GS I+ PV+Y G SV+AY P WY D+ G ++ + T+ AP +I + +RG ++P + + + N L G LYWDDGE+I + N Y Y F+
Sbjct: 17 NAAPRFDCLPEPNGNQGACEARGCIWKEDDTGKNA--------PWCYF-KDGVGYNLDSQQGSTYNLRKNGGPSNP------WGADSTEIKLTTKSIGSVLNVKIGIDGRYEPPVDF-PRETQSSTESLSLSTSFSNGVFSFSVVRQSTNRKLFDTSIGGLIFSDQFIQIATYLPSENMYGWGENTHQSLRHDFTKYLTWAMFARDQPPNSGSLDTVNLYGVHPYYMILEPDGKAHGVLIINSNAQEVTTAPGPSLIYRTIGGNLDMYFFPGPTPEMVTQQYLKFIGKPFLPAYWALGYQLSRYGYKGLAEMKTRIQAVRDAGIPIDIGVADIDYMQRYKDFTT-GDDWAGFSDYVKTM-HDWGMKLILIFDPAIEAT----YPSFQRAIAANAKFIEWETKAQVQTAIQNLYPMAKDTKIMLGVVWPDNHVAFPDFLDSTNNTQNWWINEFVNYQSQVAFDGIWIDMNEPSNFGTNQDHPWYFDSDDHPNDAPLFCPTNGSSPWEMPPYKTRAVWRFGDANSGAFLSSNTLCMLAQQDGGKQRFYNVKNLYGLTEAINTQKALFKAT-GKRGAVVSRSTYPSAGRYAGHWLGDNTARWEDLRTSVIGAQEFNLFGIPYVGSDVCGFIGTTTEELCLRWQQMGAFHSFFRNHNTIGAPAQDPAVWPSVAAATKKANLF-RYQYLPYLFSLHFTASLSGATVIRPVFFEYPTDAETFNLGYEFMWGSRILVAPVIYQGTTSVNAYLPTDRWYSLFDYRYGSIMSP----GYATVPAPTTSRIPVFVRGYSVIPRQTPSITTTATRSNPFELLIAPCPLGMGEGTLYWDDGETIVN-DFNSYDYHQFD 833
BLAST of Lysosomal alpha-glucosidase vs. Ensembl Celegans
Match: aagr-4 (Acid Alpha Glucosidase Relate [Source:UniProtKB/TrEMBL;Acc:O17352]) HSP 1 Score: 291.197 bits (744), Expect = 4.944e-84 Identity = 189/615 (30.73%), Postives = 301/615 (48.94%), Query Frame = 3
Query: 966 MNLYGAHPFYIGMPDDGKAFGVFHFNSNGQEFALQPAPA-----------------------VTYRTIGGILDFFIFTGGSPEETVKQYLELVGKPMLPPYWSLGFHLSKWGYKNIEQLSEIFQRNINAGFPIGTQWSDIDYMENYKDFTVDKEKFNGLGRFVREIKQKYNSRFVAIVDPGISCTSGNEYPPYYEGVFMDIFIKDSQTNQPIVGKVWPGETVYPDFTHPNISMYWYKQI--QRFYNDVPIDGLWIDMNEPSNFYDGSKRGCQGNSKYDNPFFVPDIRDQSLISKTICSSALHYNNEKHYNLHNLYGYTESVTTNKAL-LKTIHGKRPFILSRSTFSGSGKYTFHWTGDNQASWDQLRNSIRQVLNFNMFGIPMTGADICGFNGNSDEELCIRWMQLGAFYPFMRNHNAINQRDQDPASWNTEAQKIMKTAISNRYAMLPLFYTLLVKSHMNGGTVMRPMSFEFFSDKVTHSIELQFMIGSSIMATPVVYPGARSVDAYFPAG--VWYDFHSGKLLHSDAEGAWLTLDAPLDKINIALRGGHIVPLLDSNR-NSKSNIPNKMTLYCGMAQDMS-ASGDLYWDDGESIDSYSRNRYSYVSFNASPEQ 2720
M LYG+ P+ +G+ ++ G N+ ++P A + + G++D FI G P + +Q L G LPP ++LG+H S+W YK+ + + E+ + P+ W DI++ +N FT DK+ F ++++ K N + V IVDP I S +Y Y E +KD++ + G WPG++ Y DF +P +W +Q ++ +W DMNEPS F +G P+I T+ A H+ +H ++HN+YG+ + +T + L ++ + RPF+LSRS F+GS + WTGDN+A W L+ SI +L+ + G+P GAD+ GF GN DEEL +RW Q GAF PF R H+ + + ++P + + ++ AI RYA LP +YTL + G VMRP EF D+ + + Q+M+G+ ++ PV+ + + Y P VWYD+ + K S GA + + A L+ I + RGG I+P L + +K N + LY + Q A+G +Y DDGES +Y + ++Y F E
Sbjct: 244 MALYGSIPYLVGV-HQKRSVGALWLNAAETWVDIEPTTADKGGLSKEVLDADTKPRQVPQHNARFYSESGLIDVFITLGPQPNDIFRQLAALTGVTPLPPAFALGYHQSRWNYKDQKDVKEVHDGFVKHDIPLDVLWLDIEHTDNKAYFTFDKDAFGKPEDMIKDLADK-NRKLVTIVDPHIKKDS--KYYIYKEAKKNKYLVKDAK-DTIYEGNCWPGDSTYIDFINPKARKWWSEQFAFDKYKGTTKDVHIWNDMNEPSVF-NG-----------------PEI--------TMHKDAKHHGEFEHRDVHNVYGFHQHSSTFEGLKARSNNEVRPFVLSRSFFAGSQRTAAVWTGDNKADWAHLKQSIPMLLSLSTAGLPFVGADVGGFFGNPDEELLVRWYQAGAFQPFFRGHSHQDTKRREPWLFADNTTEAIRNAIKTRYAFLPYWYTLFYEHAKTGKPVMRPFWMEFIEDEPSWDEDRQWMVGNGLLVKPVLEEKVKELSIYLPGKRQVWYDWETHKARPS--PGA-VQIPAELNTIGLYHRGGTIIPKLSEVKLTTKENHEQPIILYIAVNQKGDFANGTIYLDDGESY-AYEKGDFAYWGFTFKREH 823
BLAST of Lysosomal alpha-glucosidase vs. Ensembl Fly
Match: GCS2alpha (gene:FBgn0027588 transcript:FBtr0070058) HSP 1 Score: 272.322 bits (695), Expect = 4.255e-77 Identity = 202/704 (28.69%), Postives = 326/704 (46.31%), Query Frame = 3
Query: 795 KSFGPLLYSDQFLQISSIMSTDQIYGIGEQNRPFLHQLSNGWERIGLWARD---NPIETFMNLYGAHPFYIGMPDDGKAFGVFHFNSNGQEFALQ--------------------PAPAVTYRTIGGILDFFIFTGGSPEETVKQYLELVGKPMLPPYWSLGFHLSKWGYKNIEQLSEIFQRNINAGFPIGTQWSDIDYMENYKDFTVDKEKFNGLGRFVREIKQKYNSRFVAIVDPGISCTSGNEYPPYYEGVFMDIFIKDSQTNQPIVGKVWPGETVYPDFTHPNISMYWYKQ--IQRFYNDVPIDGLWIDMNEPSNFYDGSKRGCQGNSKYDNPFFVPDIRDQSLISKTICSSALHYNNEKHYNLHNLYGYTESVTTNKALLKTIHGKRPFILSRSTFSGSGKYTFHWTGDNQASWDQLRNSIRQVLNFNMFGIPMTGADICGFNGNSDEELCIRWMQLGAFYPFMRNHNAINQRDQDPASWNTEAQKIMKTAISNRYAMLPLFYTLLVKSHMNGGTVMRPMSFEFFSDKVTHSIELQFMIGSSIMATPVVYPGARSVDAYFPA-------GVWYDFHSGKLLHSDAEGAWLTLDAPLDKINIALRGGHIVPLLDSNRNSKS-NIPNKMTLYCGMAQDMSASGDLYWDDGESIDSYSRNRYSYVSFNASPEQLLITPVYFG---YDLLPVLNKIVILGFEN 2798
K +GP + F S + ++GI E F+ + ++G + L+ D +++ M LYG+ P G A GV+ N+ +Q P PA + + GI+D FI G P +T KQY L G LP ++L +H S+W Y + ++ + + P+ T W DI++ + + FT DK KF ++ + + V IVDP I N Y + + ++K + N G WPG YPDF +P + Y+ Q + +F LW DMNEPS F +G P+I T +HY N +H ++HNLYG+ + + L + +RPFIL+R+ F+GS +Y WTGDN A W L++S++ L + G GAD+ F GN D EL RW Q GAF PF R H I+ + ++P + +++++ A+ RY+ LPL+YT + + G V+RP+ ++ DK ++ Q ++ ++ PV+ G VD YFPA WYD + ++++ KI + RGG IVP + R + + + + TL + + ASG LY DD +S +Y + + +V++ + +QL+ + + G Y L + +IVI G E
Sbjct: 217 KPYGPEAVALDF----SFPAAKVLFGIPEHADSFILKSTSGTDPYRLYNLDVFEYVVDSKMALYGSVPVIYGHGAQRTA-GVYWQNAAETWVDIQTSETNVVSSLVNFVSGSQKTPPPAAHFMSESGIVDAFIMLGPKPMDTFKQYAALTGTHELPQLFALAYHQSRWNYNDERDVTSVSAKFDEYNIPMDTMWLDIEHTDGKRYFTWDKFKFPHPLAMIKNLTE-LGRHLVVIVDPHIK--RDNNYFFHRDCTDRGYYVKTREGND-YEGWCWPGAASYPDFFNPVVREYYASQYALDKFQTVTADVMLWNDMNEPSVF-NG-----------------PEI--------TAPKDLIHYGNWEHRDVHNLYGHMHLMGSFAGLQQRDPNQRPFILTRAHFAGSQRYAAIWTGDNFADWSHLQHSVKMCLTEAVAGFSFCGADVGAFFGNPDTELLERWYQTGAFLPFFRAHAHIDTKRREPWLFPERTRQVIQNAVIKRYSYLPLWYTAFYELELTGEPVIRPLLAQYPLDKEAFGVDNQLLVQDRLLVRPVMQQGVSKVDVYFPAIDDKKNGDWWYDVDT---YQRQERSGYVSVPVDDFKIPVWQRGGSIVPKKERQRRASTLMLHDPYTLIICLDRQGKASGSLYLDDEKSY-AYRQGQRIHVNYEFAHDQLVNS--FVGKPKYKTLAWIERIVIAGLEK 879
BLAST of Lysosomal alpha-glucosidase vs. Ensembl Fly
Match: GCS2alpha (gene:FBgn0027588 transcript:FBtr0070062) HSP 1 Score: 272.322 bits (695), Expect = 4.255e-77 Identity = 202/704 (28.69%), Postives = 326/704 (46.31%), Query Frame = 3
Query: 795 KSFGPLLYSDQFLQISSIMSTDQIYGIGEQNRPFLHQLSNGWERIGLWARD---NPIETFMNLYGAHPFYIGMPDDGKAFGVFHFNSNGQEFALQ--------------------PAPAVTYRTIGGILDFFIFTGGSPEETVKQYLELVGKPMLPPYWSLGFHLSKWGYKNIEQLSEIFQRNINAGFPIGTQWSDIDYMENYKDFTVDKEKFNGLGRFVREIKQKYNSRFVAIVDPGISCTSGNEYPPYYEGVFMDIFIKDSQTNQPIVGKVWPGETVYPDFTHPNISMYWYKQ--IQRFYNDVPIDGLWIDMNEPSNFYDGSKRGCQGNSKYDNPFFVPDIRDQSLISKTICSSALHYNNEKHYNLHNLYGYTESVTTNKALLKTIHGKRPFILSRSTFSGSGKYTFHWTGDNQASWDQLRNSIRQVLNFNMFGIPMTGADICGFNGNSDEELCIRWMQLGAFYPFMRNHNAINQRDQDPASWNTEAQKIMKTAISNRYAMLPLFYTLLVKSHMNGGTVMRPMSFEFFSDKVTHSIELQFMIGSSIMATPVVYPGARSVDAYFPA-------GVWYDFHSGKLLHSDAEGAWLTLDAPLDKINIALRGGHIVPLLDSNRNSKS-NIPNKMTLYCGMAQDMSASGDLYWDDGESIDSYSRNRYSYVSFNASPEQLLITPVYFG---YDLLPVLNKIVILGFEN 2798
K +GP + F S + ++GI E F+ + ++G + L+ D +++ M LYG+ P G A GV+ N+ +Q P PA + + GI+D FI G P +T KQY L G LP ++L +H S+W Y + ++ + + P+ T W DI++ + + FT DK KF ++ + + V IVDP I N Y + + ++K + N G WPG YPDF +P + Y+ Q + +F LW DMNEPS F +G P+I T +HY N +H ++HNLYG+ + + L + +RPFIL+R+ F+GS +Y WTGDN A W L++S++ L + G GAD+ F GN D EL RW Q GAF PF R H I+ + ++P + +++++ A+ RY+ LPL+YT + + G V+RP+ ++ DK ++ Q ++ ++ PV+ G VD YFPA WYD + ++++ KI + RGG IVP + R + + + + TL + + ASG LY DD +S +Y + + +V++ + +QL+ + + G Y L + +IVI G E
Sbjct: 217 KPYGPEAVALDF----SFPAAKVLFGIPEHADSFILKSTSGTDPYRLYNLDVFEYVVDSKMALYGSVPVIYGHGAQRTA-GVYWQNAAETWVDIQTSETNVVSSLVNFVSGSQKTPPPAAHFMSESGIVDAFIMLGPKPMDTFKQYAALTGTHELPQLFALAYHQSRWNYNDERDVTSVSAKFDEYNIPMDTMWLDIEHTDGKRYFTWDKFKFPHPLAMIKNLTE-LGRHLVVIVDPHIK--RDNNYFFHRDCTDRGYYVKTREGND-YEGWCWPGAASYPDFFNPVVREYYASQYALDKFQTVTADVMLWNDMNEPSVF-NG-----------------PEI--------TAPKDLIHYGNWEHRDVHNLYGHMHLMGSFAGLQQRDPNQRPFILTRAHFAGSQRYAAIWTGDNFADWSHLQHSVKMCLTEAVAGFSFCGADVGAFFGNPDTELLERWYQTGAFLPFFRAHAHIDTKRREPWLFPERTRQVIQNAVIKRYSYLPLWYTAFYELELTGEPVIRPLLAQYPLDKEAFGVDNQLLVQDRLLVRPVMQQGVSKVDVYFPAIDDKKNGDWWYDVDT---YQRQERSGYVSVPVDDFKIPVWQRGGSIVPKKERQRRASTLMLHDPYTLIICLDRQGKASGSLYLDDEKSY-AYRQGQRIHVNYEFAHDQLVNS--FVGKPKYKTLAWIERIVIAGLEK 879
BLAST of Lysosomal alpha-glucosidase vs. Ensembl Fly
Match: GCS2alpha (gene:FBgn0027588 transcript:FBtr0070061) HSP 1 Score: 272.322 bits (695), Expect = 4.255e-77 Identity = 202/704 (28.69%), Postives = 326/704 (46.31%), Query Frame = 3
Query: 795 KSFGPLLYSDQFLQISSIMSTDQIYGIGEQNRPFLHQLSNGWERIGLWARD---NPIETFMNLYGAHPFYIGMPDDGKAFGVFHFNSNGQEFALQ--------------------PAPAVTYRTIGGILDFFIFTGGSPEETVKQYLELVGKPMLPPYWSLGFHLSKWGYKNIEQLSEIFQRNINAGFPIGTQWSDIDYMENYKDFTVDKEKFNGLGRFVREIKQKYNSRFVAIVDPGISCTSGNEYPPYYEGVFMDIFIKDSQTNQPIVGKVWPGETVYPDFTHPNISMYWYKQ--IQRFYNDVPIDGLWIDMNEPSNFYDGSKRGCQGNSKYDNPFFVPDIRDQSLISKTICSSALHYNNEKHYNLHNLYGYTESVTTNKALLKTIHGKRPFILSRSTFSGSGKYTFHWTGDNQASWDQLRNSIRQVLNFNMFGIPMTGADICGFNGNSDEELCIRWMQLGAFYPFMRNHNAINQRDQDPASWNTEAQKIMKTAISNRYAMLPLFYTLLVKSHMNGGTVMRPMSFEFFSDKVTHSIELQFMIGSSIMATPVVYPGARSVDAYFPA-------GVWYDFHSGKLLHSDAEGAWLTLDAPLDKINIALRGGHIVPLLDSNRNSKS-NIPNKMTLYCGMAQDMSASGDLYWDDGESIDSYSRNRYSYVSFNASPEQLLITPVYFG---YDLLPVLNKIVILGFEN 2798
K +GP + F S + ++GI E F+ + ++G + L+ D +++ M LYG+ P G A GV+ N+ +Q P PA + + GI+D FI G P +T KQY L G LP ++L +H S+W Y + ++ + + P+ T W DI++ + + FT DK KF ++ + + V IVDP I N Y + + ++K + N G WPG YPDF +P + Y+ Q + +F LW DMNEPS F +G P+I T +HY N +H ++HNLYG+ + + L + +RPFIL+R+ F+GS +Y WTGDN A W L++S++ L + G GAD+ F GN D EL RW Q GAF PF R H I+ + ++P + +++++ A+ RY+ LPL+YT + + G V+RP+ ++ DK ++ Q ++ ++ PV+ G VD YFPA WYD + ++++ KI + RGG IVP + R + + + + TL + + ASG LY DD +S +Y + + +V++ + +QL+ + + G Y L + +IVI G E
Sbjct: 217 KPYGPEAVALDF----SFPAAKVLFGIPEHADSFILKSTSGTDPYRLYNLDVFEYVVDSKMALYGSVPVIYGHGAQRTA-GVYWQNAAETWVDIQTSETNVVSSLVNFVSGSQKTPPPAAHFMSESGIVDAFIMLGPKPMDTFKQYAALTGTHELPQLFALAYHQSRWNYNDERDVTSVSAKFDEYNIPMDTMWLDIEHTDGKRYFTWDKFKFPHPLAMIKNLTE-LGRHLVVIVDPHIK--RDNNYFFHRDCTDRGYYVKTREGND-YEGWCWPGAASYPDFFNPVVREYYASQYALDKFQTVTADVMLWNDMNEPSVF-NG-----------------PEI--------TAPKDLIHYGNWEHRDVHNLYGHMHLMGSFAGLQQRDPNQRPFILTRAHFAGSQRYAAIWTGDNFADWSHLQHSVKMCLTEAVAGFSFCGADVGAFFGNPDTELLERWYQTGAFLPFFRAHAHIDTKRREPWLFPERTRQVIQNAVIKRYSYLPLWYTAFYELELTGEPVIRPLLAQYPLDKEAFGVDNQLLVQDRLLVRPVMQQGVSKVDVYFPAIDDKKNGDWWYDVDT---YQRQERSGYVSVPVDDFKIPVWQRGGSIVPKKERQRRASTLMLHDPYTLIICLDRQGKASGSLYLDDEKSY-AYRQGQRIHVNYEFAHDQLVNS--FVGKPKYKTLAWIERIVIAGLEK 879
BLAST of Lysosomal alpha-glucosidase vs. Ensembl Fly
Match: GCS2alpha (gene:FBgn0027588 transcript:FBtr0070060) HSP 1 Score: 272.322 bits (695), Expect = 4.255e-77 Identity = 202/704 (28.69%), Postives = 326/704 (46.31%), Query Frame = 3
Query: 795 KSFGPLLYSDQFLQISSIMSTDQIYGIGEQNRPFLHQLSNGWERIGLWARD---NPIETFMNLYGAHPFYIGMPDDGKAFGVFHFNSNGQEFALQ--------------------PAPAVTYRTIGGILDFFIFTGGSPEETVKQYLELVGKPMLPPYWSLGFHLSKWGYKNIEQLSEIFQRNINAGFPIGTQWSDIDYMENYKDFTVDKEKFNGLGRFVREIKQKYNSRFVAIVDPGISCTSGNEYPPYYEGVFMDIFIKDSQTNQPIVGKVWPGETVYPDFTHPNISMYWYKQ--IQRFYNDVPIDGLWIDMNEPSNFYDGSKRGCQGNSKYDNPFFVPDIRDQSLISKTICSSALHYNNEKHYNLHNLYGYTESVTTNKALLKTIHGKRPFILSRSTFSGSGKYTFHWTGDNQASWDQLRNSIRQVLNFNMFGIPMTGADICGFNGNSDEELCIRWMQLGAFYPFMRNHNAINQRDQDPASWNTEAQKIMKTAISNRYAMLPLFYTLLVKSHMNGGTVMRPMSFEFFSDKVTHSIELQFMIGSSIMATPVVYPGARSVDAYFPA-------GVWYDFHSGKLLHSDAEGAWLTLDAPLDKINIALRGGHIVPLLDSNRNSKS-NIPNKMTLYCGMAQDMSASGDLYWDDGESIDSYSRNRYSYVSFNASPEQLLITPVYFG---YDLLPVLNKIVILGFEN 2798
K +GP + F S + ++GI E F+ + ++G + L+ D +++ M LYG+ P G A GV+ N+ +Q P PA + + GI+D FI G P +T KQY L G LP ++L +H S+W Y + ++ + + P+ T W DI++ + + FT DK KF ++ + + V IVDP I N Y + + ++K + N G WPG YPDF +P + Y+ Q + +F LW DMNEPS F +G P+I T +HY N +H ++HNLYG+ + + L + +RPFIL+R+ F+GS +Y WTGDN A W L++S++ L + G GAD+ F GN D EL RW Q GAF PF R H I+ + ++P + +++++ A+ RY+ LPL+YT + + G V+RP+ ++ DK ++ Q ++ ++ PV+ G VD YFPA WYD + ++++ KI + RGG IVP + R + + + + TL + + ASG LY DD +S +Y + + +V++ + +QL+ + + G Y L + +IVI G E
Sbjct: 217 KPYGPEAVALDF----SFPAAKVLFGIPEHADSFILKSTSGTDPYRLYNLDVFEYVVDSKMALYGSVPVIYGHGAQRTA-GVYWQNAAETWVDIQTSETNVVSSLVNFVSGSQKTPPPAAHFMSESGIVDAFIMLGPKPMDTFKQYAALTGTHELPQLFALAYHQSRWNYNDERDVTSVSAKFDEYNIPMDTMWLDIEHTDGKRYFTWDKFKFPHPLAMIKNLTE-LGRHLVVIVDPHIK--RDNNYFFHRDCTDRGYYVKTREGND-YEGWCWPGAASYPDFFNPVVREYYASQYALDKFQTVTADVMLWNDMNEPSVF-NG-----------------PEI--------TAPKDLIHYGNWEHRDVHNLYGHMHLMGSFAGLQQRDPNQRPFILTRAHFAGSQRYAAIWTGDNFADWSHLQHSVKMCLTEAVAGFSFCGADVGAFFGNPDTELLERWYQTGAFLPFFRAHAHIDTKRREPWLFPERTRQVIQNAVIKRYSYLPLWYTAFYELELTGEPVIRPLLAQYPLDKEAFGVDNQLLVQDRLLVRPVMQQGVSKVDVYFPAIDDKKNGDWWYDVDT---YQRQERSGYVSVPVDDFKIPVWQRGGSIVPKKERQRRASTLMLHDPYTLIICLDRQGKASGSLYLDDEKSY-AYRQGQRIHVNYEFAHDQLVNS--FVGKPKYKTLAWIERIVIAGLEK 879
BLAST of Lysosomal alpha-glucosidase vs. Ensembl Fly
Match: GCS2alpha (gene:FBgn0027588 transcript:FBtr0070059) HSP 1 Score: 272.322 bits (695), Expect = 4.255e-77 Identity = 202/704 (28.69%), Postives = 326/704 (46.31%), Query Frame = 3
Query: 795 KSFGPLLYSDQFLQISSIMSTDQIYGIGEQNRPFLHQLSNGWERIGLWARD---NPIETFMNLYGAHPFYIGMPDDGKAFGVFHFNSNGQEFALQ--------------------PAPAVTYRTIGGILDFFIFTGGSPEETVKQYLELVGKPMLPPYWSLGFHLSKWGYKNIEQLSEIFQRNINAGFPIGTQWSDIDYMENYKDFTVDKEKFNGLGRFVREIKQKYNSRFVAIVDPGISCTSGNEYPPYYEGVFMDIFIKDSQTNQPIVGKVWPGETVYPDFTHPNISMYWYKQ--IQRFYNDVPIDGLWIDMNEPSNFYDGSKRGCQGNSKYDNPFFVPDIRDQSLISKTICSSALHYNNEKHYNLHNLYGYTESVTTNKALLKTIHGKRPFILSRSTFSGSGKYTFHWTGDNQASWDQLRNSIRQVLNFNMFGIPMTGADICGFNGNSDEELCIRWMQLGAFYPFMRNHNAINQRDQDPASWNTEAQKIMKTAISNRYAMLPLFYTLLVKSHMNGGTVMRPMSFEFFSDKVTHSIELQFMIGSSIMATPVVYPGARSVDAYFPA-------GVWYDFHSGKLLHSDAEGAWLTLDAPLDKINIALRGGHIVPLLDSNRNSKS-NIPNKMTLYCGMAQDMSASGDLYWDDGESIDSYSRNRYSYVSFNASPEQLLITPVYFG---YDLLPVLNKIVILGFEN 2798
K +GP + F S + ++GI E F+ + ++G + L+ D +++ M LYG+ P G A GV+ N+ +Q P PA + + GI+D FI G P +T KQY L G LP ++L +H S+W Y + ++ + + P+ T W DI++ + + FT DK KF ++ + + V IVDP I N Y + + ++K + N G WPG YPDF +P + Y+ Q + +F LW DMNEPS F +G P+I T +HY N +H ++HNLYG+ + + L + +RPFIL+R+ F+GS +Y WTGDN A W L++S++ L + G GAD+ F GN D EL RW Q GAF PF R H I+ + ++P + +++++ A+ RY+ LPL+YT + + G V+RP+ ++ DK ++ Q ++ ++ PV+ G VD YFPA WYD + ++++ KI + RGG IVP + R + + + + TL + + ASG LY DD +S +Y + + +V++ + +QL+ + + G Y L + +IVI G E
Sbjct: 217 KPYGPEAVALDF----SFPAAKVLFGIPEHADSFILKSTSGTDPYRLYNLDVFEYVVDSKMALYGSVPVIYGHGAQRTA-GVYWQNAAETWVDIQTSETNVVSSLVNFVSGSQKTPPPAAHFMSESGIVDAFIMLGPKPMDTFKQYAALTGTHELPQLFALAYHQSRWNYNDERDVTSVSAKFDEYNIPMDTMWLDIEHTDGKRYFTWDKFKFPHPLAMIKNLTE-LGRHLVVIVDPHIK--RDNNYFFHRDCTDRGYYVKTREGND-YEGWCWPGAASYPDFFNPVVREYYASQYALDKFQTVTADVMLWNDMNEPSVF-NG-----------------PEI--------TAPKDLIHYGNWEHRDVHNLYGHMHLMGSFAGLQQRDPNQRPFILTRAHFAGSQRYAAIWTGDNFADWSHLQHSVKMCLTEAVAGFSFCGADVGAFFGNPDTELLERWYQTGAFLPFFRAHAHIDTKRREPWLFPERTRQVIQNAVIKRYSYLPLWYTAFYELELTGEPVIRPLLAQYPLDKEAFGVDNQLLVQDRLLVRPVMQQGVSKVDVYFPAIDDKKNGDWWYDVDT---YQRQERSGYVSVPVDDFKIPVWQRGGSIVPKKERQRRASTLMLHDPYTLIICLDRQGKASGSLYLDDEKSY-AYRQGQRIHVNYEFAHDQLVNS--FVGKPKYKTLAWIERIVIAGLEK 879
BLAST of Lysosomal alpha-glucosidase vs. Ensembl Zebrafish
Match: si:ch73-12o23.1 (si:ch73-12o23.1 [Source:ZFIN;Acc:ZDB-GENE-130530-749]) HSP 1 Score: 710.294 bits (1832), Expect = 0.000e+0 Identity = 361/805 (44.84%), Postives = 496/805 (61.61%), Query Frame = 3
Query: 324 CQIANNNERLDCYPESGA--NQDSCIARGCCWSEPMRLSNKLQGVPLSIPYCYYPTDYSNYMIKSLRKSSRGIKAKLVRESPSYLPYEVAMLDLEVIYETKSRTRVRITDPKQKRYEVPMKLSPPDLKPPTDREYEVIIPSDGSFSIEIKRKSDDRSLFK-SFGPLLYSDQFLQISSIMSTDQIYGIGEQNRPFLHQLSNGWERIGLWARDNPIETFMNLYGAHPFYIGMPDDGKAFGVFHFNSNGQEFALQPAPAVTYRTIGGILDFFIFTGGSPEETVKQYLELVGKPMLPPYWSLGFHLSKWGYKNIEQLSEIFQRNINAGFPIGTQWSDIDYMENYKDFTVDKEKFNGLGRFVREIKQKYNSRFVAIVDPGISCTSG-NEYPPYYEGVFMDIFIKDSQTNQPIVGKVWPGETVYPDFTHPNISMYWYKQIQRFYNDVPIDGLWIDMNEPSNFYDGSKRGCQGNSKYDNPFFVPDIRDQSLISKTICSSALHYNNEKHYNLHNLYGYTESVTTNKALLKTIHGKRPFILSRSTFSGSGKYTFHWTGDNQASWDQLRNSIRQVLNFNMFGIPMTGADICGFNGNSDEELCIRWMQLGAFYPFMRNHNAINQRDQDPASWNTEAQKIMKTAISNRYAMLPLFYTLLVKSHMNGGTVMRPMSFEFFSDKVTHSIELQFMIGSSIMATPVVYPGARSVDAYFPAGVWYDFHSGKLLHSDAEGAWLTLDAPLDKINIALRGGHIVPLLDSNRNSKSNIPNKMTLYCGMAQDMSASGDLYWDDGESIDSYSRNRYSYVSFNASPEQLL 2726
C R DCYPE +D C AR CC+ + R + Q +P+C+Y DY +Y + S+ + G KL+R +Y P ++ L LEV++E R RV+ITDP +KRYEVP+ + P K ++ Y V + F + +KR L S PL Y+DQFLQ+SS + T IYG+GE FLH + W + +WARD P NLYG HPFY+ M DG A G F NSN + LQPAPAVT+R IGGILDF+IF G P + QYL++VGKP +P YW+LG+HL +WGYK + + + N G P QW+DIDYM+ DFT D F+ L V+++ Q+++ +V I+DPGIS + Y P+ EG IFI DS + ++GKVWPG T +PDF++P+ +WY+ +QRF+N VP DG+WIDMNEPSNF+DGS GC N + +NP + P I +L KT+C+SA HYN+H+LYG E+ T AL + I KRPFI+SRSTF GKY+ HW GDN++ W L SI +L FN+ GIP+ GADICGF G++ EELC+RW QLGAFYPF RNHN+I+++DQ+P +++ A+ MK AI RY++ P YTL +H++G TV P+ F+F +D+ T+ I+ QF+ G S++ TPV+ G V YFP G+WYDFH+G L S E + L+AP DKIN+ LR G ++P N + + L +++D A GDLYWDDGE+IDSY N+Y+Y+ F ++
Sbjct: 101 CSQVPEGWRFDCYPERNVVVTEDMCHARNCCFIQSSRGNVSAQN---GVPWCFYTPDYPSYELMSIVDTEMGKVGKLLRNKKTYYPKDIDALQLEVLFEEDHRLRVKITDPTEKRYEVPIDV-PVVHKRASNPSYTVDFIKE-PFGLIVKRTQTGAVLLNTSIAPLFYADQFLQMSSSLPTRFIYGLGEHRSNFLHDVQ--WNTLTMWARDVPPMELTNLYGVHPFYLSMESDGNAHGFFMLNSNAMDVVLQPAPAVTWRMIGGILDFYIFLGPDPSSVIGQYLDVVGKPAMPIYWALGYHLCRWGYKTSNKTWSVVKEMRNYGIPQDVQWNDIDYMDRSLDFTYDPSGFSTLPDLVKDL-QRHDQHYVMILDPGISNSQPPGSYWPFDEGKKKGIFINDSNGDI-LIGKVWPGLTAFPDFSNPDTHEWWYQNLQRFHNKVPFDGVWIDMNEPSNFFDGSLNGCPDN-ELENPPYTPGILGGTLKGKTVCASARQ-KISVHYNIHSLYGLMEAQATESAL-RRITKKRPFIISRSTFPSQGKYSGHWLGDNRSQWKDLATSIPGMLTFNILGIPLIGADICGFGGSTTEELCVRWTQLGAFYPFTRNHNSIDEQDQEPTAFSPAARTAMKEAILLRYSLFPHLYTLFHHAHVSGHTVATPLLFQFPTDEKTYGIDKQFLWGKSLLVTPVLDAGRDYVVGYFPKGLWYDFHTGNSLISSGEE--IKLEAPADKINLHLREGSVIPTQRPNTTLWVSSGQPLHLIVSLSEDGRAKGDLYWDDGETIDSYESNQYAYIYFTVEQNTMM 891
BLAST of Lysosomal alpha-glucosidase vs. Ensembl Zebrafish
Match: CABZ01079192.1 (sucrase-isomaltase (alpha-glucosidase) [Source:NCBI gene;Acc:100148922]) HSP 1 Score: 537.724 bits (1384), Expect = 8.698e-172 Identity = 317/888 (35.70%), Postives = 498/888 (56.08%), Query Frame = 3
Query: 345 ERLDCYPESGANQDSCIARGCCWSEPMRLSNKLQGVPLSIPYCYYPTDYSNYMIKSLRKSSRGIKAKLVRESPSYLPYE------VAMLDLEVIYETKSRTRVRITDPKQKRYEVPMKLSPPDLKPPTDREYEVIIPSDGSFSIEIKRKSDDRSLFKSFGP-LLYSDQFLQISSIMSTDQIYGIGEQNRP-FLHQLSNGWERIGLWARDNPIETFMNLYGAHPFYIGMPDDGKAFGVFHFNSNGQE-FALQPAPAVTYRTIGGILDFFIFTGGSPEETVKQYLE-LVGKPMLPPYWSLGFHLSKWGYKNIEQLSEIFQRNINAGFPIGTQWSDIDYMENYKDFTVDKEKFNGLGRFVREIKQKYNSRFVAIVDPGISC--TSGNEYPPYYEGVFMDIFIK--DSQTNQPIVGKVWPGE---------------------TVYPDFTHPNISMYWYKQIQRFYNDV-PIDGLWIDMNEPSNFYDGSKRG-CQGNSKYDNPFFVPDI--RDQSLISKTICSSALHY----NNEKHYNLHNLYGYTESVTTNKALLKTIHGKRPFILSRSTFSGSGKYTFHWTGDNQASWDQLRNSIRQVLNFNMFGIPMTGADICGFNGNSDEELCIRWMQLGAFYPFMRNHNAINQRDQDPASWNTEAQKIMKTAISNRYAMLPLFYTLLVKSHMNGGTVMRPMSFEFFSDKVTHSIELQFMIGSSIMATPVVYPGARSVDAYFPAGVWYDFHSGKLLHSDAEGAWLTLDAPLDKINIALRGGHIVPLLDSNRNSKSNIPNKMTLYCGMAQDMSASGDLYWDDGESIDSYSRNRYSYVSFNASPEQLL-ITPVYFGYDLLPVLNKIVILGFENKSITKFLL-----NDNDIPFHYNPET 2861
ER+DCYPE GA + C RGC W +G+PL++P CYY ++ Y ++ ++ GI A L E + P + + L +E+ + + R +I DP++ RYEVP+ L+ P+ P + + ++ F I + RK +F S P ++SDQF+QIS+ + T+ +YG GE P + H L+ + GL+A+D P +N YG HPFY+GM KA G+ NSN F + P + +R + +L ++F + +K Y + L+G+P+LP YWSLGF L ++GY N +++++++ A P Q++DIDYME DFT+DK F GL V ++ + RF+ I+DP I+ T+G+ Y + G+ D+FIK + +N + GKVWP +PDF + +W QI+ +Y++V DGLWIDMNEP++F G+ G C G+ +NP ++P + +++ L KT+C ++ +HY++HNLYG + + T AL T GKR I++RST+ SG++ HW GDN ++WDQL SI ++ F++FG+ TGADICGF + +E+C+RWMQLGAFYPF RNHN IN QDP +W E + + ++ RY +LP YTL+ ++H G TV+RPM EF +D+ T I+ QF+ GS+++ TP + G V Y P WYD+H+ +++ + +G ++ +D PLDKIN+ +RG +I+P + RN++ + N M L + A+G+L+WDDGE ID+ +Y Y F+AS L + P+L ++ +LG + S+T+ + + + F Y PET
Sbjct: 436 ERIDCYPEEGATKAECEGRGCIWEHS-------KGIPLAVPMCYYSENHG-YKASNVVENMYGITADL--EINTAFPSQRSQSPDINKLRVEITFHDEICLRWKIYDPEKARYEVPVPLNLPNPPPLWGEQTYKVEINEEPFGIRVIRKDTSEVIFDSVLPGFIFSDQFIQISTRLPTEYVYGFGETEHPSYKHDLN--FHTYGLFAKDQPPGYKLNSYGIHPFYMGMEKSKKAHGILLLNSNAMGIFTVLPFTFLFWRNVFLLLLMYVFINNTESLFMKTYNDMLIGRPVLPAYWSLGFQLCRYGYANDSEIADLYRDMRAAEIPYDVQYADIDYMERQMDFTLDKTNFLGLPALVDRMRGE-GMRFIFILDPAIAANETTGS-YAAFDSGIEKDVFIKWPPALSNDIVWGKVWPDYPNITVDNSLDWDTQVELFRSFVAFPDFFKNGTAEWWADQIKDYYDNVMKFDGLWIDMNEPASFVHGTVGGKCLGDPLLENPPYMPPLESKEKGLNHKTLCMNSEQILADGTRVRHYDVHNLYGLSHTKPTYDALHSTT-GKRGVIITRSTYPTSGRWAGHWLGDNYSAWDQLLKSIIGMMEFSIFGVSYTGADICGFFNPAQKEMCLRWMQLGAFYPFSRNHNTINMPRQDPVAWGPEFANMSRDVLNIRYTLLPYLYTLMYEAHTKGTTVVRPMLHEFVNDEKTWDIDRQFLWGSALLITPALEEGVTVVKGYLPKTRWYDYHTNEVV--EGQGQFVDMDTPLDKINLHVRGRYIIPYQNPERNTQLSRKNPMNLLVALDGSGYATGNLFWDDGEGIDTLKNRKYIYKQFSASRGSLSGVRETGLEEADRPLLGEVKVLGV-SSSVTEVTITVTGGSTTPLTFDYYPET 1305 HSP 2 Score: 270.781 bits (691), Expect = 6.571e-75 Identity = 160/390 (41.03%), Postives = 223/390 (57.18%), Query Frame = 3
Query: 1656 DMNEPSNFYDGSKRGCQGNSKYDNPFFVPDIRDQSLISKTICSSALH-YNNEKHYNLHNLYGYTESVTTNKALLKTIHGKRPFILSRSTFSGSGKYTFHWTGDNQASWDQLRNSIRQVLNFNMFGIPMTGADICGFNGNSDEELCIRWMQLGAFYPFMRNHNAINQRDQDPASWNTEAQKI--MKTAISNRYAMLPLFYTLLVKSHMNGGTVMRPMSFEFFSDKVTHSIELQFMIGSSIMATPVVYPGARSVDAYFPAGVWYDFHSGKLLHSDAEGAWLTLDAPLDKINIALRGGHI-VPLLDSNRNSKSNIPNK---MTLYCGMAQDMSASGDLYWDDGESIDSYSRNRYSYVSFNASPEQLLITPVYFGYDLLPVLN--KIVILGFEN 2798
DMNE SNF GS +GC N K + P F P I D + SKT+C A + N HY++H+LYGY+ + T +A + R + +RS+F G GKY+ HW GDN A+W+ ++ +I +L FN+FGIP GADICGF NS EELC RWMQ+GAFYPF RNHNA DQDPAS+ ++ + + ++ RY +LP YTL K+H+NG TV+RP+ EF+SD T I QFM G+ ++ TPV+ PG V+AY P +WYDF + L S + L+ NI + + + S R P++ M L + + SASG+L+WDDG+S D+ S + + F+ + L + GY L I ILGF +
Sbjct: 1 DMNEISNFIKGSNKGCADN-KLNYPPFTPRILDDLMYSKTLCMDAKQKWGN--HYDVHSLYGYSMVLATERASKEVFGTNRSMVFTRSSFPGVGKYSGHWLGDNGANWNDIKWAIPGMLEFNLFGIPYIGADICGFFDNSTEELCRRWMQVGAFYPFSRNHNAQGYEDQDPASYGLDSLLVNTSRHYLNIRYKLLPYLYTLFYKAHVNGETVVRPLMHEFYSDSQTWDIHKQFMWGAHLLITPVLDPGVHYVEAYIPDAIWYDFEN---LKSKVNFKKFKSNVNLNVNNIKMNKKNKHLNYFPSQRMKVVCSPSRRHPMGLIIALDDNKSASGELFWDDGDSRDTVSSGNHIHYQFSVTDGSLTMHVTKNGYSDPNNLKFENITILGFSS 384
BLAST of Lysosomal alpha-glucosidase vs. Ensembl Zebrafish
Match: GAA (glucosidase alpha, acid [Source:HGNC Symbol;Acc:HGNC:4065]) HSP 1 Score: 347.436 bits (890), Expect = 1.221e-107 Identity = 186/456 (40.79%), Postives = 268/456 (58.77%), Query Frame = 3
Query: 321 QCQIANNNERLDCYPESGANQDSCIARGCCWSEPMRLSNKLQGVPLSIPYCYYPTDYSNYMIKSLRKSSRGIKAKLVRESPSYLPYEVAMLDLEVIYETKSRTRVRITDPKQKRYEVP-MKLSPPDLKPPTDREYEVIIPSDGSFSIEIKRKSDDRSLF-KSFGPLLYSDQFLQISSIMSTDQIYGIGEQNRPFLHQLSNGWERIGLWARDNPIETFMNLYGAHPFYIGMPDDGKAFGVFHFNSNGQEFALQPAPAVTYRTIGGILDFFIFTGGSPEETVKQYLELVGKPMLPPYWSLGFHLSKWGYKNIEQLSEIFQRNINAGFPIGTQWSDIDYMENYKDFTVDKEKFNGLGRFVREIKQKYNSRFVAIVDPGISCTS-GNEYPPYYEGVFMDIFIKDSQTNQPIVGKVWPGETVYPDFTHPNISMYWYKQIQRFYNDVPIDGLWIDMNEPSNF 1679
QC IA R+DC + ++ C +RGCC+ P+ S L+G P +C+Y Y Y + + + RG +A L R +PSYLP ++ L L+V+ ET+ R + + DP RYEVP +K K + Y+V + F ++RKS+ R L + GPLL++DQ+LQ+S+ +++ + G+GE P L W + LW RD NLYG+HPF++ DG+A GVF NSN E +QPAPA+T+ TIGGILDFFIF G SP+ ++QY E++G PM+PPYWSLGFHL +WGY + + Q A P+ QW+D+DY + + FT D ++F L + V + Q ++V I+DPGIS S Y P+ +G+ +FI +S T Q ++GKVWPG T +PDFT+P +W I+ FYN VP+DGLWI S+F
Sbjct: 64 QCGIAVE-RRIDCARDRSLSRAECESRGCCY-LPLPQSG-LRGPP----WCFYTVFYPGYKMGPMLPTERGHRAILTRSAPSYLPRDIHTLQLDVMAETQDRLHLTLKDPTSPRYEVPFVKSQSKGHKSMENPLYDVDFQPE-PFGFSVRRKSNGRVLLNTTIGPLLFADQYLQLSTSLASSTVSGLGEHYTPITLDLD--WSSVSLWNRDMAPHRSANLYGSHPFFLVQEGDGQAHGVFLLNSNAMEVFMQPAPALTWVTIGGILDFFIFLGPSPQSVIQQYQEVIGYPMMPPYWSLGFHLCRWGYTSTNITRTVVQLMRQAKIPLDVQWNDLDYADQRRVFTFDPQRFGDLPQMVEDFHQ-LGMKYVLILDPGISSASPPGSYKPFDDGLKKGVFINNS-TGQILIGKVWPGPTAFPDFTNPTTQDWWMDWIKDFYNKVPVDGLWIVSIHSSSF 507
BLAST of Lysosomal alpha-glucosidase vs. Ensembl Zebrafish
Match: gaa (glucosidase, alpha; acid (Pompe disease, glycogen storage disease type II) [Source:ZFIN;Acc:ZDB-GENE-070212-2]) HSP 1 Score: 329.717 bits (844), Expect = 3.786e-103 Identity = 175/423 (41.37%), Postives = 255/423 (60.28%), Query Frame = 3
Query: 1659 MNEPSNFYDGSKRGCQGNSKYDNPFFVPDIRDQSLISKTICSSALHYNNEKHYNLHNLYGYTESVTTNKALLKTIHGKRPFILSRSTFSGSGKYTFHWTGDNQASWDQLRNSIRQVLNFNMFGIPMTGADICGFNGNSDEELCIRWMQLGAFYPFMRNHNAINQRDQDPASWNTEAQKIMKTAISNRYAMLPLFYTLLVKSHMNGGTVMRPMSFEFFSDKVTHSIELQFMIGSSIMATPVVYPGARSVDAYFPAGVWYDFHSGKLLHSDAEGAWLTLDAPLDKINIALRGGHIVPLLDSNRNSKSNIPNKMTLYCGMAQDMSASGDLYWDDGESIDSYSRNRYSYVSFNASPEQLLITPVYFGYDLLP-VLNKIVILGFENKSITKFLLNDNDIP-FHYNPETXXXXXXXXXXXXXXEIKIQW 2921
MNEPSNF GS GC +S+ + P + P + L S T+C SA Y + HYNLHNLYG TE++ T++ALLK + RPF+LSRS+F G G+++ HWTGD ++ W+QLR SI VL F ++GIP+ GAD+CGF G+++EELC+RW QLGAFYPFMRNHN Q+P ++ AQ M+ I+ RY++LP YTL +H + TV RP+ +F +D SI+ QF+ GSS++ +PV+ GA V AY P G WY H+G+ +S +G ++ A LD I + +R I+P + + N TL G++ A G+L+WDDGES+D++ R YSY+ F A ++ P+ L VL ++ + G + T N +P F Y +T + ++ L + ++I IQW
Sbjct: 1 MNEPSNFVQGSVDGCP-DSELEKPPYTPGVIGGQLNSGTLCVSAQQYLS-NHYNLHNLYGLTEAIATHRALLK-VKKTRPFVLSRSSFPGLGRFSAHWTGDVRSDWEQLRFSIPAVLLFGLYGIPLVGADVCGFGGDTNEELCVRWTQLGAFYPFMRNHNDRPNAPQEPYVFSQRAQDAMRMVINLRYSLLPFLYTLFHHAHTSASTVARPLFLQFPTDPDCRSIDRQFLWGSSLLISPVLEQGAVEVMAYLPPGTWYSLHNGQTYYS--KGQYIVFPASLDTIIVHVRERSIIPQQAPALTTAISRKNPFTLTVGLSVGNLAKGELFWDDGESLDTFERGDYSYLLFFAEESYVVSKPIKLNGSLDGLVLGEVRVFGVQTPP-TAVWANGQKVPDFSYRLDTKVLTVAGLELPMTAKITIQW 417
BLAST of Lysosomal alpha-glucosidase vs. Ensembl Zebrafish
Match: CABZ01088484.1 (pep chromosome:GRCz11:18:1768804:1791127:1 gene:ENSDARG00000104569.2 transcript:ENSDART00000167477.2 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:CABZ01088484.1) HSP 1 Score: 273.863 bits (699), Expect = 4.940e-82 Identity = 156/454 (34.36%), Postives = 245/454 (53.96%), Query Frame = 3
Query: 321 QCQIANNNERLDCYPESGANQDSCIARGCCWSEPMRLSNKLQGVPLSIPYCYYPTDYSNYMIKSLRKSSRGIKAKLVR-ESPSYLPYEVAMLDLEVIYETKSRTRVRITDPKQKRYEVPMKLSPPDLKPPTD-REYEVIIPSDGSFSIEIKRKSDDRSLF-KSFGPLLYSDQFLQISSIMSTDQIYGIGEQ-NRPFLHQLSNGWERIGLWARDNPIETFMNLYGAHPFYIGMPDDGKAFGVFHFNSNGQEFALQPAPAVTYRTIGGILDFFIFTGGSPEETVKQYLELVGKPMLPPYWSLGFHLSKWGYKNIEQLSEIFQRNINAGFPIGTQWSDIDYMENYKDFTVDKEKFNGLGRFVREIKQKYNSRFVAIVDPGISCTS--GNEYPPYYEGVFMDIFIKDSQTNQPIVGKVWPGETVYPDFTHPNISMYWYKQIQRFYNDVPIDGLWIDMN 1664
+C +R+DC+P+ GA+Q C+ R CCWS P+ +N +P+C++ ++ + S I+AKL R +PS ++ L +T++R R +ITD KQ R+EVP + PPT +Y+V I F ++I R + ++ LF + GPL+++DQ+LQ+S+ + + IYG+GE ++ F H W ++ RD + P I + +QPAPAVT+RTIGG+LDF++ G +PE V ++ +L+G+P +PPYWSLGF +S+W Y +++ + E+ +RN NA P Q++DIDYME K FT D KF L F + K +++ I+DP +S Y G +++ +S P+VG+VWPGETV+PDFT+ +W +I RF+ +V D LWI N
Sbjct: 37 ECPDIPLTDRVDCFPDGGASQQKCLERACCWS-PLDETN--------VPWCFFSKNHGYTADRVSNPDSTHIEAKLTRMNAPSLFGADIKDLTFHGEMQTENRFRFKITDAKQARFEVPHENVKAPANPPTGPLKYKVEI-VQKPFGVKIWRTTPEKLLFDTTIGPLVFADQYLQLSAKLPSHNIYGLGEHVHQTFRHD--TNWRTWPIFTRD-----------SFPNGISL--------------------IQPAPAVTFRTIGGVLDFYVLVGDTPEAVVDEFTKLIGRPFIPPYWSLGFQISRWDYGSLDAVKEVVERNRNASLPYDVQYTDIDYMEEKKIFTYDLVKFADLPNFADYMHDK-GQKYIVILDPAVSTGRRLNGPYDTVDRGHAAKVWVTESDGETPLVGEVWPGETVFPDFTNEACIKWWVDEISRFHKEVKHDALWIVSN 446
BLAST of Lysosomal alpha-glucosidase vs. Ensembl Xenopus
Match: GAA (glucosidase, alpha; acid [Source:NCBI gene;Acc:100495955]) HSP 1 Score: 706.827 bits (1823), Expect = 0.000e+0 Identity = 355/804 (44.15%), Postives = 510/804 (63.43%), Query Frame = 3
Query: 321 QCQIANNNERLDCYPESGANQDSCIARGCCWSEPMRLSNKLQGVPLSIPYCYYPTDYSNYMIKSLRKSSRGIKAKLVRESPSYLPYEVAMLDLEVIYETKSRTRVRITDPKQKRYEVPMKLSPPDLKPPTDREYEVIIPSDGSFSIEIKRKSDDRSLFKS-FGPLLYSDQFLQISSIMSTDQIYGIGEQNRPFLHQLSNGWERIGLWARDNPIETFMNLYGAHPFYIGMPDDGKAFGVFHFNSNGQEFALQPAPAVTYRTIGGILDFFIFTGGSPEETVKQYLELVGKPMLPPYWSLGFHLSKWGYKNIEQLSEIFQRNINAGFPIGTQWSDIDYMENYKDFTVDKEKFNGLGRFVREIKQKYNSRFVAIVDPGISCT-SGNEYPPYYEGVFMDIFIKDSQTNQPIVGKVWPGETVYPDFTHPNISMYWYKQIQRFYNDVPIDGLWIDMNEPSNFYDGSKRGCQGNSKYDNPFFVPDIRDQSLISKTICSSALHYNNEKHYNLHNLYGYTESVTTNKALLKTIHGKRPFILSRSTFSGSGKYTFHWTGDNQASWDQLRNSIRQVLNFNMFGIPMTGADICGFNGNSDEELCIRWMQLGAFYPFMRNHNAINQRDQDPASWNTEAQKIMKTAISNRYAMLPLFYTLLVKSHMNGGTVMRPMSFEFFSDKVTHSIELQFMIGSSIMATPVVYPGARSVDAYFPAGVWYDFHSGKLLHSDAEGAWLTLDAPLDKINIALRGGHIVPLLDSNRNSKSNIPNKMTLYCGMAQDMSASGDLYWDDGESIDSYSRNRYSYVSFNASPEQLL 2726
QC ++ N R DC PE +++ C ARGCC+S N Q P+C++P Y +Y + +L ++ RG A L R +++P+++ L LEV++ET+SR I DP +KRYEVP+ +P + ++V D F + I+RKS L + PL+++DQFLQIS+ + + +YG+GE LS W R+ W RD NLYG+HPFY+ M + G++ GVF NSN + LQ APA+T+RT GGILDF++F G P+ ++QY +++G P +PPYWSLGFHL +WGY ++ + +A P+ QW+DIDYM+ +DFT D+ +F V+E Q+ +++ I+DP IS + + YPPY +G+ +FI D + +P+VGKVWPG TV+PDFT P +WY ++ F++ VP DG+WIDMNEPSNF GS GC N++ +NP +VP + SL S TIC+S+ + + HYNLHNLYG +E+V T+ AL+K + KRPFI+SRSTF+ G Y HWTGD +SW+Q+ S+ +L FNMFG+PM GADICGF GN+ EELC+RW QLGAFYPFMRNHN + Q+P +++EAQK +K+A+ RY +LP YTL K+H +G TV R + EF +D T +I+ Q++ G +++ TPV+ G +V+AYFP G WY SG + S +G WL L APLDKIN+ +RGG+I+P N++ + NK+ L + D A GDL+WDDG S+ ++ R Y++V F A+ LL
Sbjct: 43 QCSVSPN-RRFDCVPEKCVSKEECEARGCCYSPAGDKGNIGQ------PWCFFPPSYPSYKMFNLTQTDRGYNATLKRSVGTFMPHDIMTLQLEVLFETESRLHFTIKDPAKKRYEVPIP-TPQVSGKASSTLFDVQFSCD-PFGLIIRRKSSGLILLNTTVAPLIFADQFLQISTSLPSGYLYGLGEHLTAL--NLSLQWNRLTFWNRDLRPSKDSNLYGSHPFYLSMEEGGQSHGVFLLNSNAMDVLLQEAPALTWRTTGGILDFYVFLGPEPKSVIRQYQDIIGYPFMPPYWSLGFHLCRWGYSTSNCTRQVVKNMRDAKIPLDVQWNDIDYMDAMRDFTYDQNRFGDFPEMVKEFHQQ-GVKYIMILDPAISSSNTPGSYPPYDDGLKRGVFITDDE-GKPLVGKVWPGLTVFPDFTMPETFSWWYDMVKLFHDQVPFDGIWIDMNEPSNFVQGSVDGCP-NNELENPPYVPGVVGGSLRSTTICASSQQHLSS-HYNLHNLYGLSEAVATHYALVKILK-KRPFIISRSTFASHGHYAGHWTGDVVSSWEQIYYSVPAILLFNMFGVPMVGADICGFGGNTTEELCVRWSQLGAFYPFMRNHNDHDSVSQEPYVFSSEAQKAIKSALLIRYTLLPYLYTLFHKAHSSGETVARALFIEFPTDPNTWTIDRQYLWGEALLITPVLEQGKTTVNAYFPTGTWYAPESGTGIQS--KGQWLMLPAPLDKINVHIRGGYILPAQVPGMNTEESRKNKLLLVVALTPDGVARGDLFWDDGVSLGTFERQDYTHVVFLAANNILL 828
BLAST of Lysosomal alpha-glucosidase vs. Ensembl Xenopus
Match: GAA (glucosidase, alpha; acid [Source:NCBI gene;Acc:100495955]) HSP 1 Score: 701.049 bits (1808), Expect = 0.000e+0 Identity = 354/805 (43.98%), Postives = 510/805 (63.35%), Query Frame = 3
Query: 321 QCQIANNNERLDCYPESGANQDSCIARGCCWSEPMRLSNKLQGVPLSIPYCYYPTDYSNYMIKSLRKSSRGIKAKLVRESPSYLPYEVAMLDLEVIYETKSRTRVRITDPKQKRYEVPMKLSPPDLKPPTDREYEVIIPSDGSFSIEIKRKSDDRSLFKSFGPLLYSDQFLQISSIMSTDQIYGIGEQNRPFLHQLSNGWERIGLWARDNPIETFMNLYGAHPFYIGMPDDGKAFGVFHFNSNGQEFALQPAPAVTYRTIGGILDFFIFTGGSPEETVKQYLELVGKPMLPPYWSLGFHLSKWGYKNIEQLSEIFQRNINAGFPIGTQWSDIDYMENYKDFTVDKEKFNGLGRFVREIKQKYNSRFVAIVDPGISCT-SGNEYPPYYEGVFMDIFIKDSQTNQPIVGKVWPGETVYPDFTHPNISMYWYKQIQRFYNDVPIDGLWIDMNEPSNFYDGSKRGCQGNSKYDNPFFVPDIRDQSLISKTICSSALHYNNEKHYNLHNLYGYTESVTTNKALLKTIHGKRPFILSRSTFSGSGKYTFHWTGDNQASWDQLRNSI--RQVLNFNMFGIPMTGADICGFNGNSDEELCIRWMQLGAFYPFMRNHNAINQRDQDPASWNTEAQKIMKTAISNRYAMLPLFYTLLVKSHMNGGTVMRPMSFEFFSDKVTHSIELQFMIGSSIMATPVVYPGARSVDAYFPAGVWYDFHSGKLLHSDAEGAWLTLDAPLDKINIALRGGHIVPLLDSNRNSKSNIPNKMTLYCGMAQDMSASGDLYWDDGESIDSYSRNRYSYVSFNASPEQLL 2726
QC ++ N R DC PE +++ C ARGCC+S N Q P+C++P Y +Y + +L ++ RG A L R +++P+++ L LEV++ET+SR I DP +KRYEVP+ +P + ++V D F + I+RKS L + PL+++DQFLQIS+ + + +YG+GE LS W R+ W RD NLYG+HPFY+ M + G++ GVF NSN + LQ APA+T+RT GGILDF++F G P+ ++QY +++G P +PPYWSLGFHL +WGY ++ + +A P+ QW+DIDYM+ +DFT D+ +F V+E Q+ +++ I+DP IS + + YPPY +G+ +FI D + +P+VGKVWPG TV+PDFT P +WY ++ F++ VP DG+WIDMNEPSNF GS GC N++ +NP +VP + SL S TIC+S+ + + HYNLHNLYG +E+V T+ AL+K + KRPFI+SRSTF+ G Y HWTGD +SW+Q+ S+ +L FNMFG+PM GADICGF GN+ EELC+RW QLGAFYPFMRNHN + Q+P +++EAQK +K+A+ RY +LP YTL K+H +G TV R + EF +D T +I+ Q++ G +++ TPV+ G +V+AYFP G WY SG + S +G WL L APLDKIN+ +RGG+I+P N++ + NK+ L + D A GDL+WDDG S+ ++ R Y++V F A+ L+
Sbjct: 68 QCSVSPN-RRFDCVPEKCVSKEECEARGCCYSPAGDKGNIGQ------PWCFFPPSYPSYKMFNLTQTDRGYNATLKRSVGTFMPHDIMTLQLEVLFETESRLHFTIKDPAKKRYEVPIP-TPQVSGKASSTLFDVQFSCD-PFGLIIRRKSS--GLNTTVAPLIFADQFLQISTSLPSGYLYGLGEHLTAL--NLSLQWNRLTFWNRDLRPSKDSNLYGSHPFYLSMEEGGQSHGVFLLNSNAMDVLLQEAPALTWRTTGGILDFYVFLGPEPKSVIRQYQDIIGYPFMPPYWSLGFHLCRWGYSTSNCTRQVVKNMRDAKIPLDVQWNDIDYMDAMRDFTYDQNRFGDFPEMVKEFHQQ-GVKYIMILDPAISSSNTPGSYPPYDDGLKRGVFITDDE-GKPLVGKVWPGLTVFPDFTMPETFSWWYDMVKLFHDQVPFDGIWIDMNEPSNFVQGSVDGCP-NNELENPPYVPGVVGGSLRSTTICASSQQHLS-SHYNLHNLYGLSEAVATHYALVKILK-KRPFIISRSTFASHGHYAGHWTGDVVSSWEQIYYSVPGMSILLFNMFGVPMVGADICGFGGNTTEELCVRWSQLGAFYPFMRNHNDHDSVSQEPYVFSSEAQKAIKSALLIRYTLLPYLYTLFHKAHSSGETVARALFIEFPTDPNTWTIDRQYLWGEALLITPVLEQGKTTVNAYFPTGTWYAPESGTGIQS--KGQWLMLPAPLDKINVHIRGGYILPAQVPGMNTEESRKNKLLLVVALTPDGVARGDLFWDDGVSLGTFERQDYTHVVFLAANVNLM 852
BLAST of Lysosomal alpha-glucosidase vs. Ensembl Xenopus
Match: GAA (glucosidase, alpha; acid [Source:NCBI gene;Acc:100495955]) HSP 1 Score: 700.664 bits (1807), Expect = 0.000e+0 Identity = 354/809 (43.76%), Postives = 508/809 (62.79%), Query Frame = 3
Query: 321 QCQIANNNERLDCYPESGANQDSCIARGCCWSEPMRLSNKLQGVPLSIPYCYYPTDYSNYMIKSLRKSSRGIKAKLVRESPSYLPYEVAMLDLEVIYETKSRTRVRITDPKQKRYEVPMKLSPPDLKPPTDREYEVIIPSDGSFSIEIKRKSDDRSLFKSFGPLLYSDQFLQISSIMSTDQIYGIGEQNRPFLHQLSNGWERIGLWARDNPIETFMNLYGAHPFYIGMPDDGKAFGVFHFNSNGQEFALQPAPAVTYRTIGGILDFFIFTGGSPEETVKQYLELVGKPMLPPYWSLGFHLSKWGYKNIEQLSEIFQRNINAGFPIGTQWSDIDYMENYKDFTVDKEKFNGLGRFVREIKQKYNSRFVAIVDPGISCT-SGNEYPPYYEGVFMDIFIKDSQTNQPIVGKVWPGETVYPDFTHPNISMYWYKQIQRFYNDVPIDGLWIDMNEPSNFYDGSKRGCQGNSKYDNPFFVPDIRDQSLISKTICSSALHYNNEKHYNLHNLYGYTESVTTNKALLKTIHGKRPFILSRSTFSGSGKYTFHWTGDNQASWDQLRNSIRQVLNFNMFGIPMTGADICGFNGNSDEELCIRWMQLGAFYPFMRNHNAINQRDQDPASWNTEAQKIMKTAISNRYAMLPLFYTLLVKSHMNGGTVMRPMSFEFFSDKVTHSIELQFMIGSSIMATPVVYPGARSVDAYFPAGVWYDFHS------GKLLHSDAEGAWLTLDAPLDKINIALRGGHIVPLLDSNRNSKSNIPNKMTLYCGMAQDMSASGDLYWDDGESIDSYSRNRYSYVSFNASPEQLL 2726
QC ++ N R DC PE +++ C ARGCC+S N Q P+C++P Y +Y + +L ++ RG A L R +++P+++ L LEV++ET+SR I DP +KRYEVP+ +P + ++V D F + I+RKS L + PL+++DQFLQIS+ + + +YG+GE LS W R+ W RD NLYG+HPFY+ M + G++ GVF NSN + LQ APA+T+RT GGILDF++F G P+ ++QY +++G P +PPYWSLGFHL +WGY ++ + +A P+ QW+DIDYM+ +DFT D+ +F V+E Q+ +++ I+DP IS + + YPPY +G+ +FI D + +P+VGKVWPG TV+PDFT P +WY ++ F++ VP DG+WIDMNEPSNF GS GC N++ +NP +VP + SL S TIC+S+ + + HYNLHNLYG +E+V T+ AL+K + KRPFI+SRSTF+ G Y HWTGD +SW+Q+ S+ +L FNMFG+PM GADICGF GN+ EELC+RW QLGAFYPFMRNHN + Q+P +++EAQK +K+A+ RY +LP YTL K+H +G TV R + EF +D T +I+ Q++ G +++ TPV+ G +V+AYFP G WY S K +G WL L APLDKIN+ +RGG+I+P N++ + NK+ L + D A GDL+WDDG S+ ++ R Y++V F A+ LL
Sbjct: 43 QCSVSPN-RRFDCVPEKCVSKEECEARGCCYSPAGDKGNIGQ------PWCFFPPSYPSYKMFNLTQTDRGYNATLKRSVGTFMPHDIMTLQLEVLFETESRLHFTIKDPAKKRYEVPIP-TPQVSGKASSTLFDVQFSCD-PFGLIIRRKSS--GLNTTVAPLIFADQFLQISTSLPSGYLYGLGEHLTAL--NLSLQWNRLTFWNRDLRPSVNSNLYGSHPFYLSMEEGGQSHGVFLLNSNAMDVLLQEAPALTWRTTGGILDFYVFLGPEPKSVIRQYQDIIGYPFMPPYWSLGFHLCRWGYSTSNCTRQVVKNMRDAKIPLDVQWNDIDYMDAMRDFTYDQNRFGDFPEMVKEFHQQ-GVKYIMILDPAISSSNTPGSYPPYDDGLKRGVFITDDE-GKPLVGKVWPGLTVFPDFTMPETFSWWYDMVKLFHDQVPFDGIWIDMNEPSNFVQGSVDGCP-NNELENPPYVPGVVGGSLRSTTICASSQQHLSS-HYNLHNLYGLSEAVATHYALVKILK-KRPFIISRSTFASHGHYAGHWTGDVVSSWEQIYYSVPAILLFNMFGVPMVGADICGFGGNTTEELCVRWSQLGAFYPFMRNHNDHDSVSQEPYVFSSEAQKAIKSALLIRYTLLPYLYTLFHKAHSSGETVARALFIEFPTDPNTWTIDRQYLWGEALLITPVLEQGKTTVNAYFPTGTWYAPESVSTPNPPKKSEKKHKGQWLMLPAPLDKINVHIRGGYILPAQVPGMNTEESRKNKLLLVVALTPDGVARGDLFWDDGVSLGTFERQDYTHVVFLAANNILL 833
BLAST of Lysosomal alpha-glucosidase vs. Ensembl Xenopus
Match: GAA (glucosidase, alpha; acid [Source:NCBI gene;Acc:100495955]) HSP 1 Score: 699.508 bits (1804), Expect = 0.000e+0 Identity = 352/805 (43.73%), Postives = 510/805 (63.35%), Query Frame = 3
Query: 321 QCQIANNNERLDCYPESGANQDSCIARGCCWSEPMRLSNKLQGVPLSIPYCYYPTDYSNYMIKSLRKSSRGIKAKLVRESPSYLPYEVAMLDLEVIYETKSRTRVRITDPKQKRYEVPMKLSPPDLKPPTDREYEVIIPSDGSFSIEIKRKSDDRSLFKSFGPLLYSDQFLQISSIMSTDQIYGIGEQNRPFLHQLSNGWERIGLWARDNPIETFMNLYGAHPFYIGMPDDGKAFGVFHFNSNGQEFALQPAPAVTYRTIGGILDFFIFTGGSPEETVKQYLELVGKPMLPPYWSLGFHLSKWGYKNIEQLSEIFQRNINAGFPIGTQWSDIDYMENYKDFTVDKEKFNGLGRFVREIKQKYNSRFVAIVDPGISCT-SGNEYPPYYEGVFMDIFIKDSQTNQPIVGKVWPGETVYPDFTHPNISMYWYKQIQRFYNDVPIDGLWIDMNEPSNFYDGSKRGCQGNSKYDNPFFVPDIRDQSLISKTICSSALHYNNEKHYNLHNLYGYTESVTTNKALLKTIHGKRPFILSRSTFSGSGKYTFHWTGDNQASWDQLRNSI--RQVLNFNMFGIPMTGADICGFNGNSDEELCIRWMQLGAFYPFMRNHNAINQRDQDPASWNTEAQKIMKTAISNRYAMLPLFYTLLVKSHMNGGTVMRPMSFEFFSDKVTHSIELQFMIGSSIMATPVVYPGARSVDAYFPAGVWYDFHSGKLLHSDAEGAWLTLDAPLDKINIALRGGHIVPLLDSNRNSKSNIPNKMTLYCGMAQDMSASGDLYWDDGESIDSYSRNRYSYVSFNASPEQLL 2726
QC ++ N R DC PE +++ C ARGCC+S N Q P+C++P Y +Y + +L ++ RG A L R +++P+++ L LEV++ET+SR I DP +KRYEVP+ +P + ++V D F + I+RKS L + PL+++DQFLQIS+ + + +YG+GE LS W R+ W RD NLYG+HPFY+ M + G++ GVF NSN + LQ APA+T+RT GGILDF++F G P+ ++QY +++G P +PPYWSLGFHL +WGY ++ + +A P+ QW+DIDYM+ +DFT D+ +F V+E Q+ +++ I+DP IS + + YPPY +G+ +FI D + +P+VGKVWPG TV+PDFT P +WY ++ F++ VP DG+WIDMNEPSNF GS GC N++ +NP +VP + SL S TIC+S+ + + HYNLHNLYG +E+V T+ AL+K + KRPFI+SRSTF+ G Y HWTGD +SW+Q+ S+ +L FNMFG+PM GADICGF GN+ EELC+RW QLGAFYPFMRNHN + Q+P +++EAQK +K+A+ RY +LP YTL K+H +G TV R + EF +D T +I+ Q++ G +++ TPV+ G +V+AYFP G WY S ++ ++G WL L APLDKIN+ +RGG+I+P N++ + NK+ L + D A GDL+WDDG S+ ++ R Y++V F A+ L+
Sbjct: 46 QCSVSPN-RRFDCVPEKCVSKEECEARGCCYSPAGDKGNIGQ------PWCFFPPSYPSYKMFNLTQTDRGYNATLKRSVGTFMPHDIMTLQLEVLFETESRLHFTIKDPAKKRYEVPIP-TPQVSGKASSTLFDVQFSCD-PFGLIIRRKSS--GLNTTVAPLIFADQFLQISTSLPSGYLYGLGEHLTAL--NLSLQWNRLTFWNRDLRPSKDSNLYGSHPFYLSMEEGGQSHGVFLLNSNAMDVLLQEAPALTWRTTGGILDFYVFLGPEPKSVIRQYQDIIGYPFMPPYWSLGFHLCRWGYSTSNCTRQVVKNMRDAKIPLDVQWNDIDYMDAMRDFTYDQNRFGDFPEMVKEFHQQ-GVKYIMILDPAISSSNTPGSYPPYDDGLKRGVFITDDE-GKPLVGKVWPGLTVFPDFTMPETFSWWYDMVKLFHDQVPFDGIWIDMNEPSNFVQGSVDGCP-NNELENPPYVPGVVGGSLRSTTICASSQQHLS-SHYNLHNLYGLSEAVATHYALVKILK-KRPFIISRSTFASHGHYAGHWTGDVVSSWEQIYYSVPGMSILLFNMFGVPMVGADICGFGGNTTEELCVRWSQLGAFYPFMRNHNDHDSVSQEPYVFSSEAQKAIKSALLIRYTLLPYLYTLFHKAHSSGETVARALFIEFPTDPNTWTIDRQYLWGEALLITPVLEQGKTTVNAYFPTGTWYAPESVRI---QSKGQWLMLPAPLDKINVHIRGGYILPAQVPGMNTEESRKNKLLLVVALTPDGVARGDLFWDDGVSLGTFERQDYTHVVFLAANVNLM 829
BLAST of Lysosomal alpha-glucosidase vs. Ensembl Xenopus
Match: GAA (glucosidase, alpha; acid [Source:NCBI gene;Acc:100495955]) HSP 1 Score: 697.967 bits (1800), Expect = 0.000e+0 Identity = 354/811 (43.65%), Postives = 511/811 (63.01%), Query Frame = 3
Query: 321 QCQIANNNERLDCYPESGANQDSCIARGCCWSEPMRLSNKLQGVPLSIPYCYYPTDYSNYMIKSLRKSSRGIKAKLVRESPSYLPYEVAMLDLEVIYETKSRTRVRITDPKQKRYEVPMKLSPPDLKPPTDREYEVIIPSDGSFSIEIKRKSDDRSLFKSFGPLLYSDQFLQISSIMSTDQIYGIGEQNRPFLHQLSNGWERIGLWARDNPIETFMNLYGAHPFYIGMPDDGKAFGVFHFNSNGQEFALQPAPAVTYRTIGGILDFFIFTGGSPEETVKQYLELVGKPMLPPYWSLGFHLSKWGYKNIEQLSEIFQRNINAGFPIGTQWSDIDYMENYKDFTVDKEKFNGLGRFVREIKQKYNSRFVAIVDPGISCT-SGNEYPPYYEGVFMDIFIKDSQTNQPIVGKVWPGETVYPDFTHPNISMYWYKQIQRFYNDVPIDGLWIDMNEPSNFYDGSKRGCQGNSKYDNPFFVPDIRDQSLISKTICSSALHYNNEKHYNLHNLYGYTESVTTNKALLKTIHGKRPFILSRSTFSGSGKYTFHWTGDNQASWDQLRNSIRQVLNFNMFGIPMTGADICGFNGNSDEELCIRWMQLGAFYPFMRNHNAINQRDQDPASWNTEAQKIMKTAISNRYAMLPLFYTLLVKSHMNGGTVMRPMSFEFFSDKVTHSIELQFMIGSSIMATPVVYPGARSVDAYFPAGVWYDFHS------GKLLHS--DAEGAWLTLDAPLDKINIALRGGHIVPLLDSNRNSKSNIPNKMTLYCGMAQDMSASGDLYWDDGESIDSYSRNRYSYVSFNASPEQLL 2726
QC ++ N R DC PE +++ C ARGCC+S N Q P+C++P Y +Y + +L ++ RG A L R +++P+++ L LEV++ET+SR I DP +KRYEVP+ +P + ++V D F + I+RKS L + PL+++DQFLQIS+ + + +YG+GE LS W R+ W RD NLYG+HPFY+ M + G++ GVF NSN + LQ APA+T+RT GGILDF++F G P+ ++QY +++G P +PPYWSLGFHL +WGY ++ + +A P+ QW+DIDYM+ +DFT D+ +F V+E Q+ +++ I+DP IS + + YPPY +G+ +FI D + +P+VGKVWPG TV+PDFT P +WY ++ F++ VP DG+WIDMNEPSNF GS GC N++ +NP +VP + SL S TIC+S+ + + HYNLHNLYG +E+V T+ AL+K + KRPFI+SRSTF+ G Y HWTGD +SW+Q+ S+ +L FNMFG+PM GADICGF GN+ EELC+RW QLGAFYPFMRNHN + Q+P +++EAQK +K+A+ RY +LP YTL K+H +G TV R + EF +D T +I+ Q++ G +++ TPV+ G +V+AYFP G WY S G + + ++G WL L APLDKIN+ +RGG+I+P N++ + NK+ L + D A GDL+WDDG S+ ++ R Y++V F A+ LL
Sbjct: 33 QCSVSPN-RRFDCVPEKCVSKEECEARGCCYSPAGDKGNIGQ------PWCFFPPSYPSYKMFNLTQTDRGYNATLKRSVGTFMPHDIMTLQLEVLFETESRLHFTIKDPAKKRYEVPIP-TPQVSGKASSTLFDVQFSCD-PFGLIIRRKSS--GLNTTVAPLIFADQFLQISTSLPSGYLYGLGEHLTAL--NLSLQWNRLTFWNRDLRPSVNSNLYGSHPFYLSMEEGGQSHGVFLLNSNAMDVLLQEAPALTWRTTGGILDFYVFLGPEPKSVIRQYQDIIGYPFMPPYWSLGFHLCRWGYSTSNCTRQVVKNMRDAKIPLDVQWNDIDYMDAMRDFTYDQNRFGDFPEMVKEFHQQ-GVKYIMILDPAISSSNTPGSYPPYDDGLKRGVFITDDE-GKPLVGKVWPGLTVFPDFTMPETFSWWYDMVKLFHDQVPFDGIWIDMNEPSNFVQGSVDGCP-NNELENPPYVPGVVGGSLRSTTICASSQQHLSS-HYNLHNLYGLSEAVATHYALVKILK-KRPFIISRSTFASHGHYAGHWTGDVVSSWEQIYYSVPAILLFNMFGVPMVGADICGFGGNTTEELCVRWSQLGAFYPFMRNHNDHDSVSQEPYVFSSEAQKAIKSALLIRYTLLPYLYTLFHKAHSSGETVARALFIEFPTDPNTWTIDRQYLWGEALLITPVLEQGKTTVNAYFPTGTWYAPESVPFWIMGSIPGTGIQSKGQWLMLPAPLDKINVHIRGGYILPAQVPGMNTEESRKNKLLLVVALTPDGVARGDLFWDDGVSLGTFERQDYTHVVFLAANNILL 825
BLAST of Lysosomal alpha-glucosidase vs. Ensembl Mouse
Match: Gaa (glucosidase, alpha, acid [Source:MGI Symbol;Acc:MGI:95609]) HSP 1 Score: 716.457 bits (1848), Expect = 0.000e+0 Identity = 364/873 (41.70%), Postives = 524/873 (60.02%), Query Frame = 3
Query: 312 ATAQCQIANNNERLDCYPESGANQDSCIARGCCWSEPMRLSNKLQGVPLSIPYCYYPTDYSNYMIKSLRKSSRGIKAKLVRESPSYLPYEVAMLDLEVIYETKSRTRVRITDPKQKRYEVPMKLSPPDLKPPTDREYEVIIPSDGSFSIEIKRKSDDRSLF-KSFGPLLYSDQFLQISSIMSTDQIYGIGEQNRPFLHQLSNGWERIGLWARDNPIETFMNLYGAHPFYIGMPDDGKAFGVFHFNSNGQEFALQPAPAVTYRTIGGILDFFIFTGGSPEETVKQYLELVGKPMLPPYWSLGFHLSKWGYKNIEQLSEIFQRNINAGFPIGTQWSDIDYMENYKDFTVDKEKFNGLGRFVREIKQKYNSRFVAIVDPGISCTS-GNEYPPYYEGVFMDIFIKDSQTNQPIVGKVWPGETVYPDFTHPNISMYWYKQIQRFYNDVPIDGLWIDMNEPSNFYDGSKRGCQGNSKYDNPFFVPDIRDQSLISKTICSSALHYNNEKHYNLHNLYGYTESVTTNKALLKTIHGKRPFILSRSTFSGSGKYTFHWTGDNQASWDQLRNSIRQVLNFNMFGIPMTGADICGFNGNSDEELCIRWMQLGAFYPFMRNHNAINQRDQDPASWNTEAQKIMKTAISNRYAMLPLFYTLLVKSHMNGGTVMRPMSFEFFSDKVTHSIELQFMIGSSIMATPVVYPGARSVDAYFPAGVWYDFHS------GKLLHS----------DAEGAWLTLDAPLDKINIALRGGHIVPLLDSNRNSKSNIPNKMTLYCGMAQDMSASGDLYWDDGESIDSYSRNRYSYVSFNASPEQL---LITPVYFGYDLLPVLNKIVILGFENKSITKFLLNDNDIP---FHYNPE 2858
A QC + ++ R DC P+ G +Q+ C ARGCC+ ++ + Q + P+C++P Y +Y +++L + G A L R SP++ P +V L LEV+ ET SR +I DP KRYEVP++ +P L Y V S+ F + ++RK R L + PL ++DQFLQ+S+ + + I G+GE P + LS W RI LW RD P NLYG+HPFY+ + D G A GVF NSN + LQP+PA+T+R+ GGILD ++F G P+ V+QYL++VG P +PPYW LGFHL +WGY + + ++ + FP+ QW+D+DYM+ +DFT +++ F VRE+ Q R++ IVDP IS Y PY EG+ +FI + +T QP++GKVWPG T +PDFT+P +W + F+ VP DG+W+DMNEPSNF GS++GC N++ +NP +VP + L + TIC+S+ H HYNLHNLYG TE++ +++AL+KT G RPF++SRSTFSG G+Y HWTGD ++SW+ L S+ +L FN+ G+P+ GADICGF G++ EELC+RW QLGAFYPFMRNHN +N Q+P ++ AQ+ M+ A + RYA+LP YTL ++H+ G TV RP+ EF D T S++ Q + G +++ TPV+ PG V YFP G WY+ G L ++G WLTL+APLD IN+ LR G+I+PL + + + M L + A G+L+WDDGES+ R Y+ V+F+A + L+ G +L L ++ +LG T+ L N IP F Y+P+
Sbjct: 78 APTQCDVPPSS-RFDCAPDKGISQEQCEARGCCYVPAGQVLKEPQ---IGQPWCFFPPSYPSYRLENLSSTESGYTATLTRTSPTFFPKDVLTLQLEVLMETDSRLHFKIKDPASKRYEVPLE-TPRVLSQAPSPLYSVEF-SEEPFGVIVRRKLGGRVLLNTTVAPLFFADQFLQLSTSLPSQHITGLGEHLSPLM--LSTDWARITLWNRDTPPSQGTNLYGSHPFYLALEDGGLAHGVFLLNSNAMDVILQPSPALTWRSTGGILDVYVFLGPEPKSVVQQYLDVVGYPFMPPYWGLGFHLCRWGYSSTAIVRQVVENMTRTHFPLDVQWNDLDYMDARRDFTFNQDSFADFPDMVRELHQD-GRRYMMIVDPAISSAGPAGSYRPYDEGLRRGVFITN-ETGQPLIGKVWPGTTAFPDFTNPETLDWWQDMVSEFHAQVPFDGMWLDMNEPSNFVRGSQQGCP-NNELENPPYVPGVVGGILQAATICASS-HQFLSTHYNLHNLYGLTEAIASSRALVKT-RGTRPFVISRSTFSGHGRYAGHWTGDVRSSWEHLAYSVPDILQFNLLGVPLVGADICGFIGDTSEELCVRWTQLGAFYPFMRNHNDLNSVPQEPYRFSETAQQAMRKAFALRYALLPYLYTLFHRAHVRGDTVARPLFLEFPEDPSTWSVDRQLLWGPALLITPVLEPGKTEVTGYFPKGTWYNMQMVSVDSLGTLPSPSSASSFRSAVQSKGQWLTLEAPLDTINVHLREGYIIPLQGPSLTTTESRKQPMALAVALTASGEADGELFWDDGESLAVLERGAYTLVTFSAKNNTIVNKLVRVTKEGAEL--QLREVTVLGVATAP-TQVL--SNGIPVSNFTYSPD 932
BLAST of Lysosomal alpha-glucosidase vs. Ensembl Mouse
Match: Gaa (glucosidase, alpha, acid [Source:MGI Symbol;Acc:MGI:95609]) HSP 1 Score: 716.457 bits (1848), Expect = 0.000e+0 Identity = 364/873 (41.70%), Postives = 524/873 (60.02%), Query Frame = 3
Query: 312 ATAQCQIANNNERLDCYPESGANQDSCIARGCCWSEPMRLSNKLQGVPLSIPYCYYPTDYSNYMIKSLRKSSRGIKAKLVRESPSYLPYEVAMLDLEVIYETKSRTRVRITDPKQKRYEVPMKLSPPDLKPPTDREYEVIIPSDGSFSIEIKRKSDDRSLF-KSFGPLLYSDQFLQISSIMSTDQIYGIGEQNRPFLHQLSNGWERIGLWARDNPIETFMNLYGAHPFYIGMPDDGKAFGVFHFNSNGQEFALQPAPAVTYRTIGGILDFFIFTGGSPEETVKQYLELVGKPMLPPYWSLGFHLSKWGYKNIEQLSEIFQRNINAGFPIGTQWSDIDYMENYKDFTVDKEKFNGLGRFVREIKQKYNSRFVAIVDPGISCTS-GNEYPPYYEGVFMDIFIKDSQTNQPIVGKVWPGETVYPDFTHPNISMYWYKQIQRFYNDVPIDGLWIDMNEPSNFYDGSKRGCQGNSKYDNPFFVPDIRDQSLISKTICSSALHYNNEKHYNLHNLYGYTESVTTNKALLKTIHGKRPFILSRSTFSGSGKYTFHWTGDNQASWDQLRNSIRQVLNFNMFGIPMTGADICGFNGNSDEELCIRWMQLGAFYPFMRNHNAINQRDQDPASWNTEAQKIMKTAISNRYAMLPLFYTLLVKSHMNGGTVMRPMSFEFFSDKVTHSIELQFMIGSSIMATPVVYPGARSVDAYFPAGVWYDFHS------GKLLHS----------DAEGAWLTLDAPLDKINIALRGGHIVPLLDSNRNSKSNIPNKMTLYCGMAQDMSASGDLYWDDGESIDSYSRNRYSYVSFNASPEQL---LITPVYFGYDLLPVLNKIVILGFENKSITKFLLNDNDIP---FHYNPE 2858
A QC + ++ R DC P+ G +Q+ C ARGCC+ ++ + Q + P+C++P Y +Y +++L + G A L R SP++ P +V L LEV+ ET SR +I DP KRYEVP++ +P L Y V S+ F + ++RK R L + PL ++DQFLQ+S+ + + I G+GE P + LS W RI LW RD P NLYG+HPFY+ + D G A GVF NSN + LQP+PA+T+R+ GGILD ++F G P+ V+QYL++VG P +PPYW LGFHL +WGY + + ++ + FP+ QW+D+DYM+ +DFT +++ F VRE+ Q R++ IVDP IS Y PY EG+ +FI + +T QP++GKVWPG T +PDFT+P +W + F+ VP DG+W+DMNEPSNF GS++GC N++ +NP +VP + L + TIC+S+ H HYNLHNLYG TE++ +++AL+KT G RPF++SRSTFSG G+Y HWTGD ++SW+ L S+ +L FN+ G+P+ GADICGF G++ EELC+RW QLGAFYPFMRNHN +N Q+P ++ AQ+ M+ A + RYA+LP YTL ++H+ G TV RP+ EF D T S++ Q + G +++ TPV+ PG V YFP G WY+ G L ++G WLTL+APLD IN+ LR G+I+PL + + + M L + A G+L+WDDGES+ R Y+ V+F+A + L+ G +L L ++ +LG T+ L N IP F Y+P+
Sbjct: 78 APTQCDVPPSS-RFDCAPDKGISQEQCEARGCCYVPAGQVLKEPQ---IGQPWCFFPPSYPSYRLENLSSTESGYTATLTRTSPTFFPKDVLTLQLEVLMETDSRLHFKIKDPASKRYEVPLE-TPRVLSQAPSPLYSVEF-SEEPFGVIVRRKLGGRVLLNTTVAPLFFADQFLQLSTSLPSQHITGLGEHLSPLM--LSTDWARITLWNRDTPPSQGTNLYGSHPFYLALEDGGLAHGVFLLNSNAMDVILQPSPALTWRSTGGILDVYVFLGPEPKSVVQQYLDVVGYPFMPPYWGLGFHLCRWGYSSTAIVRQVVENMTRTHFPLDVQWNDLDYMDARRDFTFNQDSFADFPDMVRELHQD-GRRYMMIVDPAISSAGPAGSYRPYDEGLRRGVFITN-ETGQPLIGKVWPGTTAFPDFTNPETLDWWQDMVSEFHAQVPFDGMWLDMNEPSNFVRGSQQGCP-NNELENPPYVPGVVGGILQAATICASS-HQFLSTHYNLHNLYGLTEAIASSRALVKT-RGTRPFVISRSTFSGHGRYAGHWTGDVRSSWEHLAYSVPDILQFNLLGVPLVGADICGFIGDTSEELCVRWTQLGAFYPFMRNHNDLNSVPQEPYRFSETAQQAMRKAFALRYALLPYLYTLFHRAHVRGDTVARPLFLEFPEDPSTWSVDRQLLWGPALLITPVLEPGKTEVTGYFPKGTWYNMQMVSVDSLGTLPSPSSASSFRSAVQSKGQWLTLEAPLDTINVHLREGYIIPLQGPSLTTTESRKQPMALAVALTASGEADGELFWDDGESLAVLERGAYTLVTFSAKNNTIVNKLVRVTKEGAEL--QLREVTVLGVATAP-TQVL--SNGIPVSNFTYSPD 932
BLAST of Lysosomal alpha-glucosidase vs. Ensembl Mouse
Match: Mgam (maltase-glucoamylase [Source:MGI Symbol;Acc:MGI:1203495]) HSP 1 Score: 643.269 bits (1658), Expect = 0.000e+0 Identity = 341/814 (41.89%), Postives = 495/814 (60.81%), Query Frame = 3
Query: 324 CQIANNNERLDCYPESGANQDSCIARGCCWSEPMRLSNKLQGVPLSIPYCYYPTDYSNYMIKSLRKSSRGIKAKLVRESPSYLPY--EVAMLDLEVIYETKSRTRVRITDPKQKRYEVPMK-LSPPDLKPPTDREYEVIIPSDGSFSIEIKRKSDDRSLF-KSFGPLLYSDQFLQISSIMSTDQIYGIGEQ-NRPFLHQLSNGWERIGLWARDN-PIETFMNLYGAHPFYIGMPDD-GKAFGVFHFNSNGQEFALQPAPAVTYRTIGGILDFFIFTGGSPEETVKQYLELVGKPMLPPYWSLGFHLSKWGYKNIEQLSEIFQRNINAGFPIGTQWSDIDYMENYKDFTVDKEKFNGLGRFVREIKQKYNSRFVAIVDPGISCTS--GNEYPPYYEGVFMDIFIKDSQTNQPIVGKVWPGETVYPDFTHPNISMYWYKQIQRFYNDVPIDGLWIDMNEPSNFYDGSKRGCQGNSKYDNPFFVPDIRDQSLISKTICSSALHYNNEKHYNLHNLYGYTESVTTNKALLKTIHGKRPFILSRSTFSGSGKYTFHWTGDNQASWDQLRNSIRQVLNFNMFGIPMTGADICGFNGNSDEELCIRWMQLGAFYPFMRNHNAINQRDQDPASW--NTEAQKIMKTAISNRYAMLPLFYTLLVKSHMNGGTVMRPMSFEFFSDKVTHSIELQFMIGSSIMATPVVYPGARSVDAYFPAGVWYDFHSGKLLHSDAEGAWLTLDAPLDKINIALRGGHIVPLLDSNRNSKSNIPNKMTLYCGMAQDMSASGDLYWDDGESIDSYSRNRYSYVSFNASPEQLLIT 2732
C + + ER++C P+ +N+ +C RGCCW QG +S+P CYY ++ M + ++ G A L + PS + + + L Y+T +R ++TD +KRYEVP + + P P+ Y+V + S FSI++ RKS++R LF S GPLL+SDQFLQ S+ + + +YG+GE ++ + H ++ W+ +++RD P E NLYG F++ + D+ G +FGVF NSN E LQP PA+TYRT GGILDF++F G +PE+ V++YLEL+G+P LP YW+LGF LS++ YK+++ + + +RN A P Q +DIDYM+ KDFT D F G FV+E+ + V I+DP IS S N Y PY G M I++ S P++GKVWPG TV+PD+T PN +++W K+ + F+ +V DG+WIDMNE SNF DGS GC N+ + P F P + D L SKT+C A+ + K Y++HNLYGY+ ++ T KA+ KR FI++RSTF+GSGK+ HW GDN A+W L+ SI +L FN+FGIPM GADICGF ++ EELC RWMQLGAFYPF RNHN +DQDPAS+ N+ + ++ RY +LP YTL ++H G TV RP+ EF+ D T I+ QF+ G ++ TPV+ GA V AY P WYD+ +G+ L + + + P DKI + LRGG+I P ++++ N + L + ++ A G+L+WDDGES D+ ++N Y + F+ + L +T
Sbjct: 65 CPVLSELERINCIPDQSSNKGTCDERGCCWDP--------QG-SISVP-CYYSRNHGYKMESDVVNTNAGFTATL-KNLPSAPVFGNSIENILLTAEYQTSNRFHFKLTDQTKKRYEVPHEHVQPFSGNAPSSLNYKVEV-SKEPFSIKVTRKSNNRVLFDSSIGPLLFSDQFLQFSTHLPSANVYGLGEHVHQQYRHNMN--WKTWPMFSRDTTPNEDGTNLYGVQTFFLCLEDNSGLSFGVFLMNSNAMEVTLQPTPAITYRTTGGILDFYVFLGNTPEQVVQEYLELIGRPALPSYWTLGFQLSRYDYKSLDNMKAVVERNRAAQLPYDVQHADIDYMDQKKDFTYDPVNFKGFPEFVKELHNN-GQKLVIILDPAISNNSFSSNPYGPYDRGSAMKIWVNSSDGISPVIGKVWPGTTVFPDYTSPNCAVWWTKEFELFHKEVEFDGIWIDMNEVSNFIDGSFSGCSQNN-LNYPPFTPKVLDGYLFSKTLCMDAVQHWG-KQYDVHNLYGYSMAIATAKAVKDVFPDKRSFIITRSTFAGSGKFAAHWLGDNTATWKDLQWSIPGMLEFNLFGIPMVGADICGFAQDTYEELCRRWMQLGAFYPFSRNHNGQGYKDQDPASFGNNSLLLNSSRHYLNIRYTLLPYLYTLFYRAHSRGDTVARPLLHEFYDDNNTWGIDRQFLWGPGLLITPVLDQGAEKVKAYVPNATWYDYETGEELGWRKQS--IEMQLPGDKIGLHLRGGYIFPTQQPATTTEASRKNPLGLIVALDENKEARGELFWDDGESKDTVAQNIYLFSEFSVTQNHLDVT 859 HSP 2 Score: 587.8 bits (1514), Expect = 0.000e+0 Identity = 327/896 (36.50%), Postives = 503/896 (56.14%), Query Frame = 3
Query: 345 ERLDCYP-ESGANQDSCIARGCCWSEPMRLSNKLQGVPLSIPYCYYPTDYSNYMIKSLRKSSRGIKAKLVRESPSY---LPYE-VAMLDLEVIYETKSRTRVRITDPKQKRYEVPMKLSPPD--LKPPTDREYEVIIPSDGSFSIEIKRKSDDRSLFKS-FGPLLYSDQFLQISSIMSTDQIYGIGEQNRPFLHQLSNGWERIGLWARDNPIETFMNLYGAHPFYIGMPDDGKAFGVFHFNSNGQEFALQPAPAVTYRTIGGILDFFIFTGGSPEETVKQYLELVGKPMLPPYWSLGFHLSKWGYKNIEQLSEIFQRNINAGFPIGTQWSDIDYMENYKDFTVDKEKFNGLGRFVREIKQKYNSRFVAIVDPGISCTSGNEYPPYYEGVFMDIFIKDSQTNQPIVGKVWP---------------------GETVYPDFTHPNISMYWYKQIQRFYND-------VPIDGLWIDMNEPSNFYDGSKRGCQGNSKYDNPFFVP--DIRDQSLISKTICSSALHY----NNEKHYNLHNLYGYTESVTTNKALLKTIHGKRPFILSRSTFSGSGKYTFHWTGDNQASWDQLRNSIRQVLNFNMFGIPMTGADICGFNGNSDEELCIRWMQLGAFYPFMRNHNAINQRDQDPASWNTEAQKIMKTAISNRYAMLPLFYTLLVKSHMNGGTVMRPMSFEFFSDKVTHSIELQFMIGSSIMATPVVYPGARSVDAYFPAGVWYDFHSGKLLHSDAEGAWLTLDAPLDKINIALRGGHIVPLLDSNRNSKSNIPNKMTLYCGMAQDMSASGDLYWDDGESIDSYSRNRYSYVSFNASPEQLL-----------ITPVYFGYDLLPVLNKIVILGFENKSITKFLL----NDNDIPFHYNPET 2861
ER+DCYP E GA++ +C ARGC W SN +G P CY+ + Y + +++ S G A + ++ +Y P V L L+V Y + +I DP RYEVP+ L+ P L P R Y+V+I + F I+I+RK+ ++ S ++D F++IS+ + + IYG GE ++ W G+++RD P N YG HP+Y+G+ +DG A GV NSN + QP PA+TYRTIGGILDF++F G +PE +QY EL+G+P++ PYWSLGF L ++GY+N +++ ++ + P Q+SDIDYME DF + KF+G + +K R + I+DP IS YP + GV D+FI+ + GKVWP +PDF + + +W K+I+ +++ + DGLWIDMNEPS+F +G+ ++ ++P ++P + RD+ L SKT+C + + +HY++HNLYG++++ T +A+ + + G+R +++RSTF SG++ HW GDN A+WDQL SI +++F++FGI TG+DICGF +++ E+C+RWMQLGAFYPF RNHN I R QDP SWN + I ++ + RY +LP YTL+ K+H G TV+RP+ EF SD+ T +I+ QF++G + + +PV+ P AR V+AYFP WYD++ G + +A G W TL+APL+ IN+ +RGG+I+P + N+ + M L + + A G L+WDDG+ I++ ++Y F+ + LL P+Y GY I I G + SIT + N+ ++ +YN T
Sbjct: 936 ERIDCYPDEHGASEANCSARGCIWEA----SNTTRGPP-----CYFAHEL--YSVSNVQYDSHGATADISLKASTYSNAFPSTPVNKLKLQVTYHKNEMLQFKIYDPNHSRYEVPVPLNIPSAPLSTPEGRLYDVLI-KENPFGIQIRRKTTGTVIWDSQLLGFTFNDMFIRISTRLPSTYIYGFGETEHTTF-KIDMNWHTWGMFSRDEPPGYKKNSYGVHPYYMGLEEDGNAHGVLLMNSNAMDVTFQPMPALTYRTIGGILDFYVFLGPTPEIVTQQYTELIGRPVMVPYWSLGFQLCRYGYENDTEIANLYDEMVAKQIPYDVQYSDIDYMERQLDFKLSP-KFSGFPALINRMKAN-GMRVILILDPAISGNETEPYPAFTRGVENDVFIRYPNNGSIVWGKVWPDYPNITVDPSLGWDHQVEQYRAYVAFPDFFRNSTATWWKKEIKELHSNTQDPAKSLKFDGLWIDMNEPSSFVNGAVPSGCSDATLNHPPYMPYLEARDRGLSSKTLCMESEQILPDGSRVRHYDVHNLYGWSQTRPTYEAV-QEVTGERGIVITRSTFPSSGRWGGHWLGDNTAAWDQLGKSIIGMMDFSLFGISYTGSDICGFFQDAEYEMCVRWMQLGAFYPFSRNHNTIGTRRQDPVSWNKTFEDISRSVLETRYTLLPYLYTLMYKAHTEGSTVVRPLLHEFVSDRETWNIDKQFLLGPAFLVSPVLEPNARKVEAYFPRARWYDYYKG--VDINATGEWKTLEAPLEYINLHIRGGYILPWQEPAMNTHLSRQKFMGLRAALNAEGRAEGWLFWDDGKRINT---DQYYLARFSVNQTTLLTHEKFNNYLTGTAPLYLGY--------IDIWGLSSSSITNVSISWNTNNEEVFANYNSTT 1802
BLAST of Lysosomal alpha-glucosidase vs. Ensembl Mouse
Match: Mgam (maltase-glucoamylase [Source:MGI Symbol;Acc:MGI:1203495]) HSP 1 Score: 643.269 bits (1658), Expect = 0.000e+0 Identity = 341/814 (41.89%), Postives = 495/814 (60.81%), Query Frame = 3
Query: 324 CQIANNNERLDCYPESGANQDSCIARGCCWSEPMRLSNKLQGVPLSIPYCYYPTDYSNYMIKSLRKSSRGIKAKLVRESPSYLPY--EVAMLDLEVIYETKSRTRVRITDPKQKRYEVPMK-LSPPDLKPPTDREYEVIIPSDGSFSIEIKRKSDDRSLF-KSFGPLLYSDQFLQISSIMSTDQIYGIGEQ-NRPFLHQLSNGWERIGLWARDN-PIETFMNLYGAHPFYIGMPDD-GKAFGVFHFNSNGQEFALQPAPAVTYRTIGGILDFFIFTGGSPEETVKQYLELVGKPMLPPYWSLGFHLSKWGYKNIEQLSEIFQRNINAGFPIGTQWSDIDYMENYKDFTVDKEKFNGLGRFVREIKQKYNSRFVAIVDPGISCTS--GNEYPPYYEGVFMDIFIKDSQTNQPIVGKVWPGETVYPDFTHPNISMYWYKQIQRFYNDVPIDGLWIDMNEPSNFYDGSKRGCQGNSKYDNPFFVPDIRDQSLISKTICSSALHYNNEKHYNLHNLYGYTESVTTNKALLKTIHGKRPFILSRSTFSGSGKYTFHWTGDNQASWDQLRNSIRQVLNFNMFGIPMTGADICGFNGNSDEELCIRWMQLGAFYPFMRNHNAINQRDQDPASW--NTEAQKIMKTAISNRYAMLPLFYTLLVKSHMNGGTVMRPMSFEFFSDKVTHSIELQFMIGSSIMATPVVYPGARSVDAYFPAGVWYDFHSGKLLHSDAEGAWLTLDAPLDKINIALRGGHIVPLLDSNRNSKSNIPNKMTLYCGMAQDMSASGDLYWDDGESIDSYSRNRYSYVSFNASPEQLLIT 2732
C + + ER++C P+ +N+ +C RGCCW QG +S+P CYY ++ M + ++ G A L + PS + + + L Y+T +R ++TD +KRYEVP + + P P+ Y+V + S FSI++ RKS++R LF S GPLL+SDQFLQ S+ + + +YG+GE ++ + H ++ W+ +++RD P E NLYG F++ + D+ G +FGVF NSN E LQP PA+TYRT GGILDF++F G +PE+ V++YLEL+G+P LP YW+LGF LS++ YK+++ + + +RN A P Q +DIDYM+ KDFT D F G FV+E+ + V I+DP IS S N Y PY G M I++ S P++GKVWPG TV+PD+T PN +++W K+ + F+ +V DG+WIDMNE SNF DGS GC N+ + P F P + D L SKT+C A+ + K Y++HNLYGY+ ++ T KA+ KR FI++RSTF+GSGK+ HW GDN A+W L+ SI +L FN+FGIPM GADICGF ++ EELC RWMQLGAFYPF RNHN +DQDPAS+ N+ + ++ RY +LP YTL ++H G TV RP+ EF+ D T I+ QF+ G ++ TPV+ GA V AY P WYD+ +G+ L + + + P DKI + LRGG+I P ++++ N + L + ++ A G+L+WDDGES D+ ++N Y + F+ + L +T
Sbjct: 65 CPVLSELERINCIPDQSSNKGTCDERGCCWDP--------QG-SISVP-CYYSRNHGYKMESDVVNTNAGFTATL-KNLPSAPVFGNSIENILLTAEYQTSNRFHFKLTDQTKKRYEVPHEHVQPFSGNAPSSLNYKVEV-SKEPFSIKVTRKSNNRVLFDSSIGPLLFSDQFLQFSTHLPSANVYGLGEHVHQQYRHNMN--WKTWPMFSRDTTPNEDGTNLYGVQTFFLCLEDNSGLSFGVFLMNSNAMEVTLQPTPAITYRTTGGILDFYVFLGNTPEQVVQEYLELIGRPALPSYWTLGFQLSRYDYKSLDNMKAVVERNRAAQLPYDVQHADIDYMDQKKDFTYDPVNFKGFPEFVKELHNN-GQKLVIILDPAISNNSFSSNPYGPYDRGSAMKIWVNSSDGISPVIGKVWPGTTVFPDYTSPNCAVWWTKEFELFHKEVEFDGIWIDMNEVSNFIDGSFSGCSQNN-LNYPPFTPKVLDGYLFSKTLCMDAVQHWG-KQYDVHNLYGYSMAIATAKAVKDVFPDKRSFIITRSTFAGSGKFAAHWLGDNTATWKDLQWSIPGMLEFNLFGIPMVGADICGFAQDTYEELCRRWMQLGAFYPFSRNHNGQGYKDQDPASFGNNSLLLNSSRHYLNIRYTLLPYLYTLFYRAHSRGDTVARPLLHEFYDDNNTWGIDRQFLWGPGLLITPVLDQGAEKVKAYVPNATWYDYETGEELGWRKQS--IEMQLPGDKIGLHLRGGYIFPTQQPATTTEASRKNPLGLIVALDENKEARGELFWDDGESKDTVAQNIYLFSEFSVTQNHLDVT 859 HSP 2 Score: 587.8 bits (1514), Expect = 0.000e+0 Identity = 327/896 (36.50%), Postives = 503/896 (56.14%), Query Frame = 3
Query: 345 ERLDCYP-ESGANQDSCIARGCCWSEPMRLSNKLQGVPLSIPYCYYPTDYSNYMIKSLRKSSRGIKAKLVRESPSY---LPYE-VAMLDLEVIYETKSRTRVRITDPKQKRYEVPMKLSPPD--LKPPTDREYEVIIPSDGSFSIEIKRKSDDRSLFKS-FGPLLYSDQFLQISSIMSTDQIYGIGEQNRPFLHQLSNGWERIGLWARDNPIETFMNLYGAHPFYIGMPDDGKAFGVFHFNSNGQEFALQPAPAVTYRTIGGILDFFIFTGGSPEETVKQYLELVGKPMLPPYWSLGFHLSKWGYKNIEQLSEIFQRNINAGFPIGTQWSDIDYMENYKDFTVDKEKFNGLGRFVREIKQKYNSRFVAIVDPGISCTSGNEYPPYYEGVFMDIFIKDSQTNQPIVGKVWP---------------------GETVYPDFTHPNISMYWYKQIQRFYND-------VPIDGLWIDMNEPSNFYDGSKRGCQGNSKYDNPFFVP--DIRDQSLISKTICSSALHY----NNEKHYNLHNLYGYTESVTTNKALLKTIHGKRPFILSRSTFSGSGKYTFHWTGDNQASWDQLRNSIRQVLNFNMFGIPMTGADICGFNGNSDEELCIRWMQLGAFYPFMRNHNAINQRDQDPASWNTEAQKIMKTAISNRYAMLPLFYTLLVKSHMNGGTVMRPMSFEFFSDKVTHSIELQFMIGSSIMATPVVYPGARSVDAYFPAGVWYDFHSGKLLHSDAEGAWLTLDAPLDKINIALRGGHIVPLLDSNRNSKSNIPNKMTLYCGMAQDMSASGDLYWDDGESIDSYSRNRYSYVSFNASPEQLL-----------ITPVYFGYDLLPVLNKIVILGFENKSITKFLL----NDNDIPFHYNPET 2861
ER+DCYP E GA++ +C ARGC W SN +G P CY+ + Y + +++ S G A + ++ +Y P V L L+V Y + +I DP RYEVP+ L+ P L P R Y+V+I + F I+I+RK+ ++ S ++D F++IS+ + + IYG GE ++ W G+++RD P N YG HP+Y+G+ +DG A GV NSN + QP PA+TYRTIGGILDF++F G +PE +QY EL+G+P++ PYWSLGF L ++GY+N +++ ++ + P Q+SDIDYME DF + KF+G + +K R + I+DP IS YP + GV D+FI+ + GKVWP +PDF + + +W K+I+ +++ + DGLWIDMNEPS+F +G+ ++ ++P ++P + RD+ L SKT+C + + +HY++HNLYG++++ T +A+ + + G+R +++RSTF SG++ HW GDN A+WDQL SI +++F++FGI TG+DICGF +++ E+C+RWMQLGAFYPF RNHN I R QDP SWN + I ++ + RY +LP YTL+ K+H G TV+RP+ EF SD+ T +I+ QF++G + + +PV+ P AR V+AYFP WYD++ G + +A G W TL+APL+ IN+ +RGG+I+P + N+ + M L + + A G L+WDDG+ I++ ++Y F+ + LL P+Y GY I I G + SIT + N+ ++ +YN T
Sbjct: 936 ERIDCYPDEHGASEANCSARGCIWEA----SNTTRGPP-----CYFAHEL--YSVSNVQYDSHGATADISLKASTYSNAFPSTPVNKLKLQVTYHKNEMLQFKIYDPNHSRYEVPVPLNIPSAPLSTPEGRLYDVLI-KENPFGIQIRRKTTGTVIWDSQLLGFTFNDMFIRISTRLPSTYIYGFGETEHTTF-KIDMNWHTWGMFSRDEPPGYKKNSYGVHPYYMGLEEDGNAHGVLLMNSNAMDVTFQPMPALTYRTIGGILDFYVFLGPTPEIVTQQYTELIGRPVMVPYWSLGFQLCRYGYENDTEIANLYDEMVAKQIPYDVQYSDIDYMERQLDFKLSP-KFSGFPALINRMKAN-GMRVILILDPAISGNETEPYPAFTRGVENDVFIRYPNNGSIVWGKVWPDYPNITVDPSLGWDHQVEQYRAYVAFPDFFRNSTATWWKKEIKELHSNTQDPAKSLKFDGLWIDMNEPSSFVNGAVPSGCSDATLNHPPYMPYLEARDRGLSSKTLCMESEQILPDGSRVRHYDVHNLYGWSQTRPTYEAV-QEVTGERGIVITRSTFPSSGRWGGHWLGDNTAAWDQLGKSIIGMMDFSLFGISYTGSDICGFFQDAEYEMCVRWMQLGAFYPFSRNHNTIGTRRQDPVSWNKTFEDISRSVLETRYTLLPYLYTLMYKAHTEGSTVVRPLLHEFVSDRETWNIDKQFLLGPAFLVSPVLEPNARKVEAYFPRARWYDYYKG--VDINATGEWKTLEAPLEYINLHIRGGYILPWQEPAMNTHLSRQKFMGLRAALNAEGRAEGWLFWDDGKRINT---DQYYLARFSVNQTTLLTHEKFNNYLTGTAPLYLGY--------IDIWGLSSSSITNVSISWNTNNEEVFANYNSTT 1802
BLAST of Lysosomal alpha-glucosidase vs. Ensembl Mouse
Match: Mgam (maltase-glucoamylase [Source:MGI Symbol;Acc:MGI:1203495]) HSP 1 Score: 642.499 bits (1656), Expect = 0.000e+0 Identity = 341/814 (41.89%), Postives = 495/814 (60.81%), Query Frame = 3
Query: 324 CQIANNNERLDCYPESGANQDSCIARGCCWSEPMRLSNKLQGVPLSIPYCYYPTDYSNYMIKSLRKSSRGIKAKLVRESPSYLPY--EVAMLDLEVIYETKSRTRVRITDPKQKRYEVPMK-LSPPDLKPPTDREYEVIIPSDGSFSIEIKRKSDDRSLF-KSFGPLLYSDQFLQISSIMSTDQIYGIGEQ-NRPFLHQLSNGWERIGLWARDN-PIETFMNLYGAHPFYIGMPDD-GKAFGVFHFNSNGQEFALQPAPAVTYRTIGGILDFFIFTGGSPEETVKQYLELVGKPMLPPYWSLGFHLSKWGYKNIEQLSEIFQRNINAGFPIGTQWSDIDYMENYKDFTVDKEKFNGLGRFVREIKQKYNSRFVAIVDPGISCTS--GNEYPPYYEGVFMDIFIKDSQTNQPIVGKVWPGETVYPDFTHPNISMYWYKQIQRFYNDVPIDGLWIDMNEPSNFYDGSKRGCQGNSKYDNPFFVPDIRDQSLISKTICSSALHYNNEKHYNLHNLYGYTESVTTNKALLKTIHGKRPFILSRSTFSGSGKYTFHWTGDNQASWDQLRNSIRQVLNFNMFGIPMTGADICGFNGNSDEELCIRWMQLGAFYPFMRNHNAINQRDQDPASW--NTEAQKIMKTAISNRYAMLPLFYTLLVKSHMNGGTVMRPMSFEFFSDKVTHSIELQFMIGSSIMATPVVYPGARSVDAYFPAGVWYDFHSGKLLHSDAEGAWLTLDAPLDKINIALRGGHIVPLLDSNRNSKSNIPNKMTLYCGMAQDMSASGDLYWDDGESIDSYSRNRYSYVSFNASPEQLLIT 2732
C + + ER++C P+ +N+ +C RGCCW QG +S+P CYY ++ M + ++ G A L + PS + + + L Y+T +R ++TD +KRYEVP + + P P+ Y+V + S FSI++ RKS++R LF S GPLL+SDQFLQ S+ + + +YG+GE ++ + H ++ W+ +++RD P E NLYG F++ + D+ G +FGVF NSN E LQP PA+TYRT GGILDF++F G +PE+ V++YLEL+G+P LP YW+LGF LS++ YK+++ + + +RN A P Q +DIDYM+ KDFT D F G FV+E+ + V I+DP IS S N Y PY G M I++ S P++GKVWPG TV+PD+T PN +++W K+ + F+ +V DG+WIDMNE SNF DGS GC N+ + P F P + D L SKT+C A+ + K Y++HNLYGY+ ++ T KA+ KR FI++RSTF+GSGK+ HW GDN A+W L+ SI +L FN+FGIPM GADICGF ++ EELC RWMQLGAFYPF RNHN +DQDPAS+ N+ + ++ RY +LP YTL ++H G TV RP+ EF+ D T I+ QF+ G ++ TPV+ GA V AY P WYD+ +G+ L + + + P DKI + LRGG+I P ++++ N + L + ++ A G+L+WDDGES D+ ++N Y + F+ + L +T
Sbjct: 65 CPVLSELERINCIPDQSSNKGTCDERGCCWDP--------QG-SISVP-CYYSRNHGYKMESDVVNTNAGFTATL-KNLPSAPVFGNSIENILLTAEYQTSNRFHFKLTDQTKKRYEVPHEHVQPFSGNAPSSLNYKVEV-SKEPFSIKVTRKSNNRVLFDSSIGPLLFSDQFLQFSTHLPSANVYGLGEHVHQQYRHNMN--WKTWPMFSRDTTPNEDGTNLYGVQTFFLCLEDNSGLSFGVFLMNSNAMEVTLQPTPAITYRTTGGILDFYVFLGNTPEQVVQEYLELIGRPALPSYWTLGFQLSRYDYKSLDNMKAVVERNRAAQLPYDVQHADIDYMDQKKDFTYDPVNFKGFPEFVKELHNN-GQKLVIILDPAISNNSFSSNPYGPYDRGSAMKIWVNSSDGISPVIGKVWPGTTVFPDYTSPNCAVWWTKEFELFHKEVEFDGIWIDMNEVSNFIDGSFSGCSQNN-LNYPPFTPKVLDGYLFSKTLCMDAVQHWG-KQYDVHNLYGYSMAIATAKAVKDVFPDKRSFIITRSTFAGSGKFAAHWLGDNTATWKDLQWSIPGMLEFNLFGIPMVGADICGFAQDTYEELCRRWMQLGAFYPFSRNHNGQGYKDQDPASFGNNSLLLNSSRHYLNIRYTLLPYLYTLFYRAHSRGDTVARPLLHEFYDDNNTWGIDRQFLWGPGLLITPVLDQGAEKVKAYVPNATWYDYETGEELGWRKQS--IEMQLPGDKIGLHLRGGYIFPTQQPATTTEASRKNPLGLIVALDENKEARGELFWDDGESKDTVAQNIYLFSEFSVTQNHLDVT 859 HSP 2 Score: 590.112 bits (1520), Expect = 0.000e+0 Identity = 310/838 (36.99%), Postives = 487/838 (58.11%), Query Frame = 3
Query: 345 ERLDCYP-ESGANQDSCIARGCCWSEPMRLSNKLQGVPLSIPYCYYPTDYSNYMIKSLRKSSRGIKAKLVRESPSY---LPYE-VAMLDLEVIYETKSRTRVRITDPKQKRYEVPMKLSPPD--LKPPTDREYEVIIPSDGSFSIEIKRKSDDRSLFKS-FGPLLYSDQFLQISSIMSTDQIYGIGEQNRPFLHQLSNGWERIGLWARDNPIETFMNLYGAHPFYIGMPDDGKAFGVFHFNSNGQEFALQPAPAVTYRTIGGILDFFIFTGGSPEETVKQYLELVGKPMLPPYWSLGFHLSKWGYKNIEQLSEIFQRNINAGFPIGTQWSDIDYMENYKDFTVDKEKFNGLGRFVREIKQKYNSRFVAIVDPGISCTSGNEYPPYYEGVFMDIFIKDSQTNQPIVGKVWP---------------------GETVYPDFTHPNISMYWYKQIQRFYND-------VPIDGLWIDMNEPSNFYDGSKRGCQGNSKYDNPFFVPDI--RDQSLISKTICSSALHY----NNEKHYNLHNLYGYTESVTTNKALLKTIHGKRPFILSRSTFSGSGKYTFHWTGDNQASWDQLRNSIRQVLNFNMFGIPMTGADICGFNGNSDEELCIRWMQLGAFYPFMRNHNAINQRDQDPASWNTEAQKIMKTAISNRYAMLPLFYTLLVKSHMNGGTVMRPMSFEFFSDKVTHSIELQFMIGSSIMATPVVYPGARSVDAYFPAGVWYDFHSGKLLHSDAEGAWLTLDAPLDKINIALRGGHIVPLLDSNRNSKSNIPNKMTLYCGMAQDMSASGDLYWDDGESIDSYSRNRYSYVSFNASPEQLLIT 2732
ER+DCYP E GA++ +C ARGC W SN +G P CY+ + Y + +++ S G A + ++ +Y P V L L+V Y + +I DP RYEVP+ L+ P L P R Y+V+I + F I+I+RK+ ++ S ++D F++IS+ + + IYG GE ++ W G+++RD P N YG HP+Y+G+ +DG A GV NSN + QP PA+TYRTIGGILDF++F G +PE +QY EL+G+P++ PYWSLGF L ++GY+N +++ ++ + P Q+SDIDYME DF + KF+G + +K R + I+DP IS YP + +GV D+FI+ + GKVWP +PDF + +++W K+I+ +++ + DGLWIDMNEPS+F +G+ ++ ++P ++P + RD+ L SKT+C + H + +HY++H+LYG++++ T +A+ + + G+R +++RSTF SG++ HW GDN A+WDQL SI +++F++FGI TG+DICGF +++ E+C+RWMQLGAFYPF RNHN I + QDP SW+ + I ++ + RY +LP YTL+ K+H G TV+RP+ EF +D+ T +I+ QF++G + + +PV+ P AR+V AYFP WYD+++G ++S W TL APL+ IN+ +RGG+I+P N+ + + L + + A G+L+WDDG+S D+ ++N Y + F+ + L +T
Sbjct: 936 ERIDCYPDEHGASEANCSARGCIWEA----SNTTRGPP-----CYFAHEL--YSVSNVQYDSHGATADISLKASTYSNAFPSTPVNKLKLQVTYHKNEMLQFKIYDPNHSRYEVPVPLNIPSAPLSTPEGRLYDVLI-KENPFGIQIRRKTTGTVIWDSQLLGFTFNDMFIRISTRLPSTYIYGFGETEHTTF-KIDMNWHTWGMFSRDEPPGYKKNSYGVHPYYMGLEEDGNAHGVLLMNSNAMDVTFQPMPALTYRTIGGILDFYVFLGPTPEIVTQQYTELIGRPVMVPYWSLGFQLCRYGYENDTEIANLYDEMVAKQIPYDVQYSDIDYMERQLDFKLSP-KFSGFPALINRMKAN-GMRVILILDPAISGNETQPYPAFTQGVENDVFIRYPNNGGIVWGKVWPDYPNITVDSSLDWDIQVERYRAYVAFPDFFRDSTALWWKKEIKELHSNSQDQAKSLKFDGLWIDMNEPSSFVNGAVPSGCSDATLNHPPYMPHVEGRDRGLSSKTLCMESEHILPDGSRVRHYDVHSLYGWSQTRPTYEAM-QEVTGERGIVITRSTFPSSGRWGGHWLGDNTAAWDQLGKSIIGMMDFSLFGISYTGSDICGFFQDAEYEMCVRWMQLGAFYPFSRNHNTIGTKRQDPVSWDKTFEDISRSVLETRYTLLPYLYTLMYKAHKEGSTVVRPLLHEFLADRETWNIDKQFLLGPAFLVSPVLEPNARNVTAYFPKAQWYDYYTGADINST---GWTTLPAPLEHINLHVRGGYILPWQQPALNTNLSRKKPLGLLIALDEKKEARGELFWDDGQSKDTVAKNIYLFSEFSVTQNHLDVT 1754 HSP 3 Score: 589.726 bits (1519), Expect = 0.000e+0 Identity = 326/898 (36.30%), Postives = 505/898 (56.24%), Query Frame = 3
Query: 339 NNERLDCYPES-GANQDSCIARGCCWSEPMRLSNKLQGVPLSIPYCYYPTDYSNYMIKSLRKSSRGIKAKL-VRESP---SYLPYEVAMLDLEVIYETKSRTRVRITDPKQKRYEVPMKLSPPDL--KPPTDREYEVIIPSDGSFSIEIKRKSDDRSLFKS-FGPLLYSDQFLQISSIMSTDQIYGIGEQNRPFLHQLSNGWERIGLWARDNPIETFMNLYGAHPFYIGMPDDGKAFGVFHFNSNGQEFALQPAPAVTYRTIGGILDFFIFTGGSPEETVKQYLELVGKPMLPPYWSLGFHLSKWGYKNIEQLSEIFQRNINAGFPIGTQWSDIDYMENYKDFTVDKEKFNGLGRFVREIKQKYNSRFVAIVDPGISCTSGNEYPPYYEGVFMDIFIKDSQTNQPIVGKVWP---------------------GETVYPDFTHPNISMYWYKQIQRFYND-------VPIDGLWIDMNEPSNFYDGSKRGCQGNSKYDNPFFVP--DIRDQSLISKTICSSALHY----NNEKHYNLHNLYGYTESVTTNKALLKTIHGKRPFILSRSTFSGSGKYTFHWTGDNQASWDQLRNSIRQVLNFNMFGIPMTGADICGFNGNSDEELCIRWMQLGAFYPFMRNHNAINQRDQDPASWNTEAQKIMKTAISNRYAMLPLFYTLLVKSHMNGGTVMRPMSFEFFSDKVTHSIELQFMIGSSIMATPVVYPGARSVDAYFPAGVWYDFHSGKLLHSDAEGAWLTLDAPLDKINIALRGGHIVPLLDSNRNSKSNIPNKMTLYCGMAQDMSASGDLYWDDGESIDSYSRNRYSYVSFNASPEQLL-----------ITPVYFGYDLLPVLNKIVILGFENKSITKFLL----NDNDIPFHYNPET 2861
+ E++DCYP+ GA++ SC ARGC W E SN +P+CY+ + Y + +++ S G A + ++ SP ++ V L L+V Y + +I DP RYEVP+ L+ P P R Y+V+I + F IEI+RK ++ S +SD F++IS+ + + IYG GE ++ W + G+++RD P N YG HP+Y+G+ +DG A GV NSN + QP PA+TYRT GGILDF++F G +PE +QY EL+G+P++ PYWSLGF L ++GY+N +++ ++ ++ P Q+SDIDYME DF + KF+G + +K R + I+DP IS YP + GV D+FI+ + GKVWP +PDF + + +W K+I+ +++ + DGLWIDMNEPS+F +G+ ++ ++P ++P + RD+ L SKT+C + + +HY++HNLYG++++ T +A+ + + G+R +++RSTF SG++ HW GDN A+WDQL SI +++F++FGI TG+DICGF +++ E+C+RWMQLGAFYPF RNHN I R QDP SWN + I ++ + RY +LP YTL+ K+H G TV+RP+ EF SD+ T +I+ QF++G + + +PV+ P AR V+AYFP WYD++ G + +A G W TL+APL+ IN+ +RGG+I+P + N+ + M L + + A G L+WDDG+ I++ ++Y F+ + LL P+Y GY I I G + SIT + N+ ++ +YN T
Sbjct: 2723 DEEKIDCYPDQHGASETSCTARGCVWEE----SNSD-----VVPFCYFVNEL--YSVSNVQYGSHGATANISLKASPYSGAFPSTPVKELQLQVTYHKNEMLQFKIYDPNHSRYEVPVPLNIPSAPSSTPESRLYDVLI-KENPFGIEIRRKGTGTVVWDSQLLGFTFSDMFIRISTRLPSTYIYGFGETEHTTF-KIDMNWNKWGMFSRDEPPGYRKNSYGVHPYYMGLEEDGNAHGVLLMNSNAMDVTFQPMPALTYRTTGGILDFYVFLGPTPEFVTQQYTELIGRPVMVPYWSLGFQLCRYGYENDSEIASLYDEMVDKKIPYDVQYSDIDYMERQLDFKLSP-KFSGFPALINRMKAN-GMRVILILDPAISGNETEPYPAFTRGVENDVFIRYPNNGSIVWGKVWPDYPNITVDPSLGWDHQVEQYRAYVAFPDFFRNSTATWWKKEIKELHSNTQDPAKSLKFDGLWIDMNEPSSFVNGAVPSGCSDATLNHPPYMPYLEARDRGLSSKTLCMESEQILPDGSRVRHYDVHNLYGWSQTRPTYEAV-QEVTGERGIVITRSTFPSSGRWGGHWLGDNTAAWDQLGKSIIGMMDFSLFGISYTGSDICGFFQDAEYEMCVRWMQLGAFYPFSRNHNTIGTRRQDPVSWNKTFEDISRSVLETRYTLLPYLYTLMYKAHTEGSTVVRPLLHEFVSDRETWNIDKQFLLGPAFLVSPVLEPNARKVEAYFPRARWYDYYKG--VDINATGEWKTLEAPLEYINLHIRGGYILPWQEPAMNTHLSRQKFMGLRAALNAEGRAEGWLFWDDGKRINT---DQYYLARFSVNQTTLLTHEKFNNYLTGTAPLYLGY--------IDIWGLSSSSITNVSISWNTNNEEVFANYNSTT 3591 HSP 4 Score: 587.415 bits (1513), Expect = 0.000e+0 Identity = 308/821 (37.52%), Postives = 479/821 (58.34%), Query Frame = 3
Query: 345 ERLDCYP-ESGANQDSCIARGCCWSEPMRLSNKLQGVPLSIPYCYYPTDYSNYMIKSLRKSSRGIKAKLVRESPSY---LPYE-VAMLDLEVIYETKSRTRVRITDPKQKRYEVPMKLSPPDLKPPTD--REYEVIIPSDGSFSIEIKRKSDDRSLFKS-FGPLLYSDQFLQISSIMSTDQIYGIGEQNRPFLHQLSNGWERIGLWARDNPIETFMNLYGAHPFYIGMPDDGKAFGVFHFNSNGQEFALQPAPAVTYRTIGGILDFFIFTGGSPEETVKQYLELVGKPMLPPYWSLGFHLSKWGYKNIEQLSEIFQRNINAGFPIGTQWSDIDYMENYKDFTVDKEKFNGLGRFVREIKQKYNSRFVAIVDPGISCTSGNEYPPYYEGVFMDIFIKDSQTNQPIVGKVWP---------------------GETVYPDFTHPNISMYWYKQIQRFYND-------VPIDGLWIDMNEPSNFYDGSKRGCQGNSKYDNPFFVPDI--RDQSLISKTICSSALHY----NNEKHYNLHNLYGYTESVTTNKALLKTIHGKRPFILSRSTFSGSGKYTFHWTGDNQASWDQLRNSIRQVLNFNMFGIPMTGADICGFNGNSDEELCIRWMQLGAFYPFMRNHNAINQRDQDPASWNTEAQKIMKTAISNRYAMLPLFYTLLVKSHMNGGTVMRPMSFEFFSDKVTHSIELQFMIGSSIMATPVVYPGARSVDAYFPAGVWYDFHSGKLLHSDAEGAWLTLDAPLDKINIALRGGHIVPLLDSNRNSKSNIPNKMTLYCGMAQDMSASGDLYWDDGESIDSYSRN 2681
ER+DCYP E GA++ +C ARGC W E SN + +P CY+ + Y + +++ S G A + ++ +Y P V L L+V Y + +I DP RYEVP+ L+ P T+ R Y+V+I + F I+++RKS ++ S ++D F++IS+ + + IYG GE ++ W G+++RD P N YG HP+Y+G+ +DG A GV NSN + QP PA+TYRT GGILDF++ G +PE +QY EL+G+P++ PYWSLGF L ++GY+N +++ ++ ++ P Q+SDIDYME DF + KF+G + +K R + I+DP IS YP + GV D+FI+ + GKVWP +PDF + + +W K+I+ Y + + DGLWIDMNEPS+F +G+ ++ ++P ++P + RD+ L SKT+C + H + +HY++H+LYG++++ T +A+ + + G+R +++RSTF SG++ HW GDN A+WDQL SI +++F++FGI TG+DICGF +++ E+C+RWMQLGAFYPF RNHN I + QDP SWN + I ++ + RY +LP YTL+ K+HM G TV+RP+ EF SD+ T +I+ QF++G + + +PV+ P AR++ AYFP +WYD+++G ++S E W TL APL+ IN+ +RGG+I+P N+ + N + L + ++ A G+L+WDDG+S D + N
Sbjct: 1831 ERIDCYPDEHGASEANCSARGCIWEE----SNTI-----GVPTCYFAHEL--YSVSNVQYDSHGATADISLKASTYSNAFPSTPVNKLKLQVTYHKNEMLQFKIYDPNNSRYEVPVPLNIPSAPSSTNEGRLYDVLI-KENPFGIQVRRKSTGTVIWDSQLLGFTFNDMFIRISTRLPSTYIYGFGETEHTTF-KIDMNWHTWGMFSRDEPPGYKKNSYGVHPYYMGLEEDGNAHGVLLMNSNAMDVTFQPMPALTYRTTGGILDFYVLLGPTPEIVTQQYTELIGRPVMVPYWSLGFQLCRYGYENDSEIASLYDEMVDKQIPYDVQYSDIDYMERQLDFKLSP-KFSGFPALINRMKAN-GMRVILILDPAISGNETQPYPAFTRGVENDVFIRYPNNGSIVWGKVWPDYPNITVDPSLGWDHQVEQYRAYVAFPDFFRNSTATWWKKEIEELYTNTEEPEKSLKFDGLWIDMNEPSSFVNGAVPSGCSDATLNHPPYMPHVEGRDRGLSSKTLCMESEHILPDGSRVQHYDVHSLYGWSQTRPTYEAV-QEVTGERGIVITRSTFPSSGRWGGHWLGDNTAAWDQLGKSIIGMMDFSLFGISYTGSDICGFFQDAEYEMCVRWMQLGAFYPFSRNHNTIGTKRQDPVSWNKTFEDISRSVLETRYTLLPYLYTLMYKAHMEGSTVVRPLLHEFVSDRKTWNIDKQFLLGPAFLVSPVLEPNARNISAYFPTALWYDYYTGANINSTGE--WKTLPAPLEHINLHVRGGYILPWQRPALNTHLSRKNPLGLLIALDENKEARGELFWDDGQSKDLTTNN 2633
BLAST of Lysosomal alpha-glucosidase vs. UniProt/SwissProt
Match: sp|Q6P7A9|LYAG_RAT (Lysosomal alpha-glucosidase OS=Rattus norvegicus OX=10116 GN=Gaa PE=2 SV=1) HSP 1 Score: 721.465 bits (1861), Expect = 0.000e+0 Identity = 365/871 (41.91%), Postives = 524/871 (60.16%), Query Frame = 3
Query: 318 AQCQIANNNERLDCYPESGANQDSCIARGCCWSEPMRLSNKLQGVPLSIPYCYYPTDYSNYMIKSLRKSSRGIKAKLVRESPSYLPYEVAMLDLEVIYETKSRTRVRITDPKQKRYEVPMKLSPPDLKPPTDREYEVIIPSDGSFSIEIKRKSDDRSLF-KSFGPLLYSDQFLQISSIMSTDQIYGIGEQNRPFLHQLSNGWERIGLWARDNPIETFMNLYGAHPFYIGMPDDGKAFGVFHFNSNGQEFALQPAPAVTYRTIGGILDFFIFTGGSPEETVKQYLELVGKPMLPPYWSLGFHLSKWGYKNIEQLSEIFQRNINAGFPIGTQWSDIDYMENYKDFTVDKEKFNGLGRFVREIKQKYNSRFVAIVDPGISCTS-GNEYPPYYEGVFMDIFIKDSQTNQPIVGKVWPGETVYPDFTHPNISMYWYKQIQRFYNDVPIDGLWIDMNEPSNFYDGSKRGCQGNSKYDNPFFVPDIRDQSLISKTICSSALHYNNEKHYNLHNLYGYTESVTTNKALLKTIHGKRPFILSRSTFSGSGKYTFHWTGDNQASWDQLRNSIRQVLNFNMFGIPMTGADICGFNGNSDEELCIRWMQLGAFYPFMRNHNAINQRDQDPASWNTEAQKIMKTAISNRYAMLPLFYTLLVKSHMNGGTVMRPMSFEFFSDKVTHSIELQFMIGSSIMATPVVYPGARSVDAYFPAGVWYDFH------------------SGKLLHSDAEGAWLTLDAPLDKINIALRGGHIVPLLDSNRNSKSNIPNKMTLYCGMAQDMSASGDLYWDDGESIDSYSRNRYSYVSFNASPEQLLITPVYF---GYDLLPVLNKIVILGFENKSITKFLLNDNDIP-FHYNPE 2858
QC + N+ R DC P+ G Q+ C ARGCCW + L G + P+C++P Y +Y +++L + G A L R SP++ P +V L LEV+ ET SR I DP KRYEVP++ +P L Y V S+ F + ++RK R L + PL ++DQFLQ+S+ + + I G+GE P + LS W RI LW RD +NLYG+HPFY+ + D G A GVF NSN + LQP+PA+T+R+ GGILD ++F G P+ V+QYL++VG P +PPYW LGFHL +WGY + + ++ + FP+ QW+D+DYM+ +DFT +++ F V E+ Q R++ IVDP IS + Y PY EG+ +FI + +T QP++GKVWPG T +PDFT+P +W + F+ VP DG+WIDMNEPSNF GS++GC N + +NP +VP + +L + TIC+S+ H HYNLHNLYG TE++ +++AL+KT G RPF++SRSTF+G G+Y HWTGD +SW+ L S+ ++L FN+ G+P+ GADICGF GN+ EELC+RW QLGAFYPFMRNHN +N Q+P ++ AQ+ M+ A + RYA+LP YTL +H+ G TV RP+ EF D T S++ Q + G +++ TPV+ PG V YFP G+WY+ ++HS +G WLTL+APLD IN+ LR G+I+PL + + + M L + + ASG+L+WDDGES+ R Y+ V+F+A ++ V+ G +L L ++ ILG + T+ L N + F Y+P+
Sbjct: 80 TQCDVTPNS-RFDCAPDKGITQEQCEARGCCW---VPAGQVLNGPVMGQPWCFFPPSYPSYRLENLSSTESGYTATLTRTSPTFFPKDVLTLQLEVLMETDSRLHFMIKDPTSKRYEVPLE-TPRVLSQAPSPLYSVEF-SEEPFGVIVRRKLGGRVLLNTTVAPLFFADQFLQLSTSLPSQHIAGLGEHLSPLM--LSTEWTRITLWNRDVAPSQGVNLYGSHPFYLALEDGGLAHGVFLLNSNAMDVVLQPSPALTWRSTGGILDVYVFLGPEPKSVVQQYLDVVGYPFMPPYWGLGFHLCRWGYSSTAIVRQVVENMTRTHFPLDVQWNDLDYMDARRDFTFNQDGFADFPDMVHELHQG-GRRYMMIVDPAISSSGPAGSYRPYDEGLRRGVFITN-ETGQPLIGKVWPGSTAFPDFTNPETLDWWQDMVSEFHAQVPFDGMWIDMNEPSNFIRGSQQGCPDN-ELENPPYVPGVVGGALQAATICASS-HQFLSTHYNLHNLYGLTEAIASSRALVKT-RGTRPFVISRSTFAGHGRYAGHWTGDVWSSWEHLAYSVPEILQFNLLGVPLVGADICGFQGNTTEELCVRWTQLGAFYPFMRNHNDLNSLPQEPYRFSETAQQAMRKAFTLRYALLPYLYTLFHGAHVKGDTVARPLFLEFPEDPSTWSVDRQLLWGPALLITPVLEPGKTDVTGYFPKGMWYNLQMVPVETLGSLPSSSPASSFRSIVHS--KGQWLTLEAPLDTINVHLRAGYIIPLQGPSLTTTESRKQPMALAVALTESGEASGELFWDDGESLGVLERGAYTLVTFSAKNNTIVNKLVHVTKEGGEL--QLREVTILGV-TTAPTQVLSNGISVSNFTYSPD 932
BLAST of Lysosomal alpha-glucosidase vs. UniProt/SwissProt
Match: sp|P70699|LYAG_MOUSE (Lysosomal alpha-glucosidase OS=Mus musculus OX=10090 GN=Gaa PE=1 SV=2) HSP 1 Score: 716.457 bits (1848), Expect = 0.000e+0 Identity = 364/873 (41.70%), Postives = 524/873 (60.02%), Query Frame = 3
Query: 312 ATAQCQIANNNERLDCYPESGANQDSCIARGCCWSEPMRLSNKLQGVPLSIPYCYYPTDYSNYMIKSLRKSSRGIKAKLVRESPSYLPYEVAMLDLEVIYETKSRTRVRITDPKQKRYEVPMKLSPPDLKPPTDREYEVIIPSDGSFSIEIKRKSDDRSLF-KSFGPLLYSDQFLQISSIMSTDQIYGIGEQNRPFLHQLSNGWERIGLWARDNPIETFMNLYGAHPFYIGMPDDGKAFGVFHFNSNGQEFALQPAPAVTYRTIGGILDFFIFTGGSPEETVKQYLELVGKPMLPPYWSLGFHLSKWGYKNIEQLSEIFQRNINAGFPIGTQWSDIDYMENYKDFTVDKEKFNGLGRFVREIKQKYNSRFVAIVDPGISCTS-GNEYPPYYEGVFMDIFIKDSQTNQPIVGKVWPGETVYPDFTHPNISMYWYKQIQRFYNDVPIDGLWIDMNEPSNFYDGSKRGCQGNSKYDNPFFVPDIRDQSLISKTICSSALHYNNEKHYNLHNLYGYTESVTTNKALLKTIHGKRPFILSRSTFSGSGKYTFHWTGDNQASWDQLRNSIRQVLNFNMFGIPMTGADICGFNGNSDEELCIRWMQLGAFYPFMRNHNAINQRDQDPASWNTEAQKIMKTAISNRYAMLPLFYTLLVKSHMNGGTVMRPMSFEFFSDKVTHSIELQFMIGSSIMATPVVYPGARSVDAYFPAGVWYDFHS------GKLLHS----------DAEGAWLTLDAPLDKINIALRGGHIVPLLDSNRNSKSNIPNKMTLYCGMAQDMSASGDLYWDDGESIDSYSRNRYSYVSFNASPEQL---LITPVYFGYDLLPVLNKIVILGFENKSITKFLLNDNDIP---FHYNPE 2858
A QC + ++ R DC P+ G +Q+ C ARGCC+ ++ + Q + P+C++P Y +Y +++L + G A L R SP++ P +V L LEV+ ET SR +I DP KRYEVP++ +P L Y V S+ F + ++RK R L + PL ++DQFLQ+S+ + + I G+GE P + LS W RI LW RD P NLYG+HPFY+ + D G A GVF NSN + LQP+PA+T+R+ GGILD ++F G P+ V+QYL++VG P +PPYW LGFHL +WGY + + ++ + FP+ QW+D+DYM+ +DFT +++ F VRE+ Q R++ IVDP IS Y PY EG+ +FI + +T QP++GKVWPG T +PDFT+P +W + F+ VP DG+W+DMNEPSNF GS++GC N++ +NP +VP + L + TIC+S+ H HYNLHNLYG TE++ +++AL+KT G RPF++SRSTFSG G+Y HWTGD ++SW+ L S+ +L FN+ G+P+ GADICGF G++ EELC+RW QLGAFYPFMRNHN +N Q+P ++ AQ+ M+ A + RYA+LP YTL ++H+ G TV RP+ EF D T S++ Q + G +++ TPV+ PG V YFP G WY+ G L ++G WLTL+APLD IN+ LR G+I+PL + + + M L + A G+L+WDDGES+ R Y+ V+F+A + L+ G +L L ++ +LG T+ L N IP F Y+P+
Sbjct: 78 APTQCDVPPSS-RFDCAPDKGISQEQCEARGCCYVPAGQVLKEPQ---IGQPWCFFPPSYPSYRLENLSSTESGYTATLTRTSPTFFPKDVLTLQLEVLMETDSRLHFKIKDPASKRYEVPLE-TPRVLSQAPSPLYSVEF-SEEPFGVIVRRKLGGRVLLNTTVAPLFFADQFLQLSTSLPSQHITGLGEHLSPLM--LSTDWARITLWNRDTPPSQGTNLYGSHPFYLALEDGGLAHGVFLLNSNAMDVILQPSPALTWRSTGGILDVYVFLGPEPKSVVQQYLDVVGYPFMPPYWGLGFHLCRWGYSSTAIVRQVVENMTRTHFPLDVQWNDLDYMDARRDFTFNQDSFADFPDMVRELHQD-GRRYMMIVDPAISSAGPAGSYRPYDEGLRRGVFITN-ETGQPLIGKVWPGTTAFPDFTNPETLDWWQDMVSEFHAQVPFDGMWLDMNEPSNFVRGSQQGCP-NNELENPPYVPGVVGGILQAATICASS-HQFLSTHYNLHNLYGLTEAIASSRALVKT-RGTRPFVISRSTFSGHGRYAGHWTGDVRSSWEHLAYSVPDILQFNLLGVPLVGADICGFIGDTSEELCVRWTQLGAFYPFMRNHNDLNSVPQEPYRFSETAQQAMRKAFALRYALLPYLYTLFHRAHVRGDTVARPLFLEFPEDPSTWSVDRQLLWGPALLITPVLEPGKTEVTGYFPKGTWYNMQMVSVDSLGTLPSPSSASSFRSAVQSKGQWLTLEAPLDTINVHLREGYIIPLQGPSLTTTESRKQPMALAVALTASGEADGELFWDDGESLAVLERGAYTLVTFSAKNNTIVNKLVRVTKEGAEL--QLREVTVLGVATAP-TQVL--SNGIPVSNFTYSPD 932
BLAST of Lysosomal alpha-glucosidase vs. UniProt/SwissProt
Match: sp|Q5R7A9|LYAG_PONAB (Lysosomal alpha-glucosidase OS=Pongo abelii OX=9601 GN=GAA PE=2 SV=1) HSP 1 Score: 701.049 bits (1808), Expect = 0.000e+0 Identity = 357/870 (41.03%), Postives = 527/870 (60.57%), Query Frame = 3
Query: 321 QCQIANNNERLDCYPESGANQDSCIARGCCWSEPMRLSNKLQGVPLSIPYCYYPTDYSNYMIKSLRKSSRGIKAKLVRESPSYLPYEVAMLDLEVIYETKSRTRVRITDPKQKRYEVPMKLSPPDLKPPTDREYEVIIPSDGSFSIEIKRKSDDRSLF-KSFGPLLYSDQFLQISSIMSTDQIYGIGEQNRPFLHQLSNGWERIGLWARDNPIETFMNLYGAHPFYIGMPDDGKAFGVFHFNSNGQEFALQPAPAVTYRTIGGILDFFIFTGGSPEETVKQYLELVGKPMLPPYWSLGFHLSKWGYKNIEQLSEIFQRNINAGFPIGTQWSDIDYMENYKDFTVDKEKFNGLGRFVREIKQKYNSRFVAIVDPGISCTS-GNEYPPYYEGVFMDIFIKDSQTNQPIVGKVWPGETVYPDFTHPNISMYWYKQIQRFYNDVPIDGLWIDMNEPSNFYDGSKRGCQGNSKYDNPFFVPDIRDQSLISKTICSSALHYNNEKHYNLHNLYGYTESVTTNKALLKTIHGKRPFILSRSTFSGSGKYTFHWTGDNQASWDQLRNSIRQVLNFNMFGIPMTGADICGFNGNSDEELCIRWMQLGAFYPFMRNHNAINQRDQDPASWNTEAQKIMKTAISNRYAMLPLFYTLLVKSHMNGGTVMRPMSFEFFSDKVTHSIELQFMIGSSIMATPVVYPGARSVDAYFPAGVWYDFHS------GKL-----------LHSDAEGAWLTLDAPLDKINIALRGGHIVPLLDSNRNSKSNIPNKMTLYCGMAQDMSASGDLYWDDGESIDSYSRNRYSYVSFNASPEQLLITPVYFGYDLLPV-LNKIVILGFENKSITKFLLNDNDIP---FHYNPET 2861
QC + N+ R DC P+ ++ C ARGCC+ + L+G + P+C++P Y +Y +++L S G A L R +P++ P ++ L L+V+ ET++R I DP +RYEVP++ + P+ Y V S+ F + ++R+ D R L + PL ++DQFLQ+S+ + + I G+ E P + LS W R+ LW RD NLYG+HPFY+ + D G A GVF NSN + LQP+PA+++R+ GGILD +IF G P+ V+QYL++VG P +PPYW LGFHL +WGY + S++ + A FP+ QW+D+DYM+ +DFT +K+ F VRE+ Q R++ IVDP IS + Y PY EG+ +FI + +T+QP++GKVWPG T +PDFT+P +W + F++ VP DG+WIDMNEPSNF GS+ GC N + +NP +VP + +L + TIC+S+ H HYNLHNLYG TE++ +++AL+K G RPF++SRSTF+G G+Y HWTGD +SW+QL +S+ ++L FN+ G+P+ GAD+CGF GN+ EELC+RW QLGAFYPFMRNHN + Q+P S++ AQ+ M+ A++ RYA+LP YTL ++H+ G TV RP+ EF D T +++ Q + G +++ TPV+ G V YFP G+WYD + G L +HS EG W+TL APLD IN+ LR G+I+PL + + M L + + A G+L+WDDGES++ R Y+ V F A ++ V+ + + L K+ +LG + T + N +P F Y+P+T
Sbjct: 81 QCDVPPNS-RFDCAPDKAITREQCDARGCCY---IPAKQGLRGAQMGQPWCFFPPSYPSYKLENLSSSEMGYTATLTRTTPTFFPKDILTLRLDVMMETENRLHFTIKDPANRRYEVPLETPRVHSRAPSPL-YSVEF-SEEPFGVIVRRQLDGRVLLNTTVAPLFFADQFLQLSTSLPSQYITGLAEHLSPLM--LSTSWTRVTLWNRDLAPTPGANLYGSHPFYLALEDGGSAHGVFLLNSNAMDVVLQPSPALSWRSTGGILDVYIFLGPEPKSVVRQYLDVVGYPFMPPYWGLGFHLCRWGYSSTAITSQVVENMTRAHFPLDVQWNDLDYMDARRDFTFNKDGFRDFPAMVRELHQG-GRRYMMIVDPAISSSGPAGSYRPYDEGLRRGVFITN-ETSQPLIGKVWPGSTAFPDFTNPAALAWWEDMVAEFHDQVPFDGMWIDMNEPSNFIRGSEDGCPDN-ELENPPYVPGVVGGTLQAATICASS-HQFLSTHYNLHNLYGLTEAIASHRALVKA-RGTRPFVISRSTFAGHGRYAGHWTGDVWSSWEQLASSVPEILQFNLLGVPLVGADVCGFLGNTSEELCVRWTQLGAFYPFMRNHNGLLNLPQEPYSFSEPAQQAMRKALTLRYALLPHLYTLFHQAHVAGETVARPLFLEFPKDSSTWTVDHQLLWGEALLITPVLQAGKAEVTGYFPLGIWYDLQTVPIEALGSLPPPPAAPREPAIHS--EGQWVTLPAPLDTINVHLRAGYIIPLQGPGLTTTESRQQPMALAVALTKGGEARGELFWDDGESLEVLERGAYTQVLFLARNNTIVNELVHVTSEGAGLQLQKVTVLGV---ATTPQQVLSNGVPVSNFTYSPDT 932
BLAST of Lysosomal alpha-glucosidase vs. UniProt/SwissProt
Match: sp|P10253|LYAG_HUMAN (Lysosomal alpha-glucosidase OS=Homo sapiens OX=9606 GN=GAA PE=1 SV=4) HSP 1 Score: 699.508 bits (1804), Expect = 0.000e+0 Identity = 359/872 (41.17%), Postives = 523/872 (59.98%), Query Frame = 3
Query: 321 QCQIANNNERLDCYPESGANQDSCIARGCCWSEPMRLSNKLQGVPLSIPYCYYPTDYSNYMIKSLRKSSRGIKAKLVRESPSYLPYEVAMLDLEVIYETKSRTRVRITDPKQKRYEVPMKLSPPDLKPPTDREYEVIIPSDGSFSIEIKRKSDDRSLF-KSFGPLLYSDQFLQISSIMSTDQIYGIGEQNRPFLHQLSNGWERIGLWARDNPIETFMNLYGAHPFYIGMPDDGKAFGVFHFNSNGQEFALQPAPAVTYRTIGGILDFFIFTGGSPEETVKQYLELVGKPMLPPYWSLGFHLSKWGYKNIEQLSEIFQRNINAGFPIGTQWSDIDYMENYKDFTVDKEKFNGLGRFVREIKQKYNSRFVAIVDPGISCTS-GNEYPPYYEGVFMDIFIKDSQTNQPIVGKVWPGETVYPDFTHPNISMYWYKQIQRFYNDVPIDGLWIDMNEPSNFYDGSKRGCQGNSKYDNPFFVPDIRDQSLISKTICSSALHYNNEKHYNLHNLYGYTESVTTNKALLKTIHGKRPFILSRSTFSGSGKYTFHWTGDNQASWDQLRNSIRQVLNFNMFGIPMTGADICGFNGNSDEELCIRWMQLGAFYPFMRNHNAINQRDQDPASWNTEAQKIMKTAISNRYAMLPLFYTLLVKSHMNGGTVMRPMSFEFFSDKVTHSIELQFMIGSSIMATPVVYPGARSVDAYFPAGVWYDFHS------GKL-----------LHSDAEGAWLTLDAPLDKINIALRGGHIVPLLDSNRNSKSNIPNKMTLYCGMAQDMSASGDLYWDDGESIDSYSRNRYSYVSFNASPEQL---LITPVYFGYDLLPVLNKIVILGFENKSITKFLLNDNDIP---FHYNPET 2861
QC + N+ R DC P+ Q+ C ARGCC+ + LQG + P+C++P Y +Y +++L S G A L R +P++ P ++ L L+V+ ET++R I DP +RYEVP++ + P+ Y V S+ F + ++R+ D R L + PL ++DQFLQ+S+ + + I G+ E P + LS W RI LW RD NLYG+HPFY+ + D G A GVF NSN + LQP+PA+++R+ GGILD +IF G P+ V+QYL++VG P +PPYW LGFHL +WGY + ++ + A FP+ QW+D+DYM++ +DFT +K+ F V+E+ Q R++ IVDP IS + Y PY EG+ +FI + +T QP++GKVWPG T +PDFT+P +W + F++ VP DG+WIDMNEPSNF GS+ GC N++ +NP +VP + +L + TIC+S+ H HYNLHNLYG TE++ +++AL+K G RPF++SRSTF+G G+Y HWTGD +SW+QL +S+ ++L FN+ G+P+ GAD+CGF GN+ EELC+RW QLGAFYPFMRNHN++ Q+P S++ AQ+ M+ A++ RYA+LP YTL ++H+ G TV RP+ EF D T +++ Q + G +++ TPV+ G V YFP G WYD + G L +HS EG W+TL APLD IN+ LR G+I+PL + + M L + + A G+L+WDDGES++ R Y+ V F A + L+ G L L K+ +LG N +P F Y+P+T
Sbjct: 81 QCDVPPNS-RFDCAPDKAITQEQCEARGCCY---IPAKQGLQGAQMGQPWCFFPPSYPSYKLENLSSSEMGYTATLTRTTPTFFPKDILTLRLDVMMETENRLHFTIKDPANRRYEVPLETPHVHSRAPSPL-YSVEF-SEEPFGVIVRRQLDGRVLLNTTVAPLFFADQFLQLSTSLPSQYITGLAEHLSPLM--LSTSWTRITLWNRDLAPTPGANLYGSHPFYLALEDGGSAHGVFLLNSNAMDVVLQPSPALSWRSTGGILDVYIFLGPEPKSVVQQYLDVVGYPFMPPYWGLGFHLCRWGYSSTAITRQVVENMTRAHFPLDVQWNDLDYMDSRRDFTFNKDGFRDFPAMVQELHQG-GRRYMMIVDPAISSSGPAGSYRPYDEGLRRGVFITN-ETGQPLIGKVWPGSTAFPDFTNPTALAWWEDMVAEFHDQVPFDGMWIDMNEPSNFIRGSEDGCP-NNELENPPYVPGVVGGTLQAATICASS-HQFLSTHYNLHNLYGLTEAIASHRALVKA-RGTRPFVISRSTFAGHGRYAGHWTGDVWSSWEQLASSVPEILQFNLLGVPLVGADVCGFLGNTSEELCVRWTQLGAFYPFMRNHNSLLSLPQEPYSFSEPAQQAMRKALTLRYALLPHLYTLFHQAHVAGETVARPLFLEFPKDSSTWTVDHQLLWGEALLITPVLQAGKAEVTGYFPLGTWYDLQTVPVEALGSLPPPPAAPREPAIHS--EGQWVTLPAPLDTINVHLRAGYIIPLQGPGLTTTESRQQPMALAVALTKGGEARGELFWDDGESLEVLERGAYTQVIFLARNNTIVNELVRVTSEGAGL--QLQKVTVLGVATAPQQVL---SNGVPVSNFTYSPDT 932
BLAST of Lysosomal alpha-glucosidase vs. UniProt/SwissProt
Match: sp|Q9MYM4|LYAG_BOVIN (Lysosomal alpha-glucosidase OS=Bos taurus OX=9913 GN=GAA PE=2 SV=1) HSP 1 Score: 671.003 bits (1730), Expect = 0.000e+0 Identity = 337/816 (41.30%), Postives = 489/816 (59.93%), Query Frame = 3
Query: 312 ATAQCQIANNNERLDCYPESGANQDSCIARGCCWSEPMRLSNKLQGVPLSIPYCYYPTDYSNYMIKSLRKSSRGIKAKLVRESPSYLPYEVAMLDLEVIYETKSRTRVRITDPKQKRYEVPMKLSPPDLKPPTDREYEVIIPSDGSFSIEIKRKSDDRSLF-KSFGPLLYSDQFLQISSIMSTDQIYGIGEQNRPFLHQLSNGWERIGLWARDNPIETFMNLYGAHPFYIGMPDDGKAFGVFHFNSNGQEFALQPAPAVTYRTIGGILDFFIFTGGSPEETVKQYLELVGKPMLPPYWSLGFHLSKWGYKNIEQLSEIFQRNINAGFPIGTQWSDIDYMENYKDFTVDKEKFNGLGRFVREIKQKYNSRFVAIVDPGISCTS-GNEYPPYYEGVFMDIFIKDSQTNQPIVGKVWPGETVYPDFTHPNISMYWYKQIQRFYNDVPIDGLWIDMNEPSNFYDGSKRGCQGNSKYDNPFFVPDIRDQSLISKTICSSALHYNNEKHYNLHNLYGYTESVTTNKALLKTIHGKRPFILSRSTFSGSGKYTFHWTGDNQASWDQLRNSIRQVLNFNMFGIPMTGADICGFNGNSDEELCIRWMQLGAFYPFMRNHNAINQRDQDPASWNTEAQKIMKTAISNRYAMLPLFYTLLVKSHMNGGTVMRPMSFEFFSDKVTHSIELQFMIGSSIMATPVVYPGARSVDAYFPAGVWYDFHS---------------GKLLHSDAEGAWLTLDAPLDKINIALRGGHIVPLLDSNRNSKSNIPNKMTLYCGMAQDMSASGDLYWDDGESIDSYSRNRYSYVSFNA 2708
A QC + N+ R DC P+ G C ARGCC+ P Q + P+C++P Y +Y +++L + G A L R P++ P ++ L L+++ ET+SR I DP +RYEVP++ +P Y V S+ F + ++RK D R L + PL ++DQFLQ+S+ + + I G+ E + LS W +I LW RD E +NLYG+HPFY+ + D G A GVF NSN + LQP+PA+++R+ GGILD +IF G P+ V+QYL++VG P +PPYW LGFHL +WGY ++ + A FP+ QW+D+DYM+ +DFT +K+ F V+E+ Q R++ IVDP IS + Y PY EG+ +FI + +T QP++G+VWPG T +PDFT+P +W + F+ VP DG+WIDMNEPSNF GS GC NS +NP ++P + +L + TIC+S+ H HY+LHNLYG TE++ +++AL+K G RPF++SRSTF+G G+Y+ HWTGD ++W+QL S+ ++L FN+ G+P+ GADICGF GN+ EELC+RW QLGAFYPFMRNHNA+N + Q+P ++ AQ+ M+ A + RY +LP YTL ++H+ G TV RP+ EF D T +++ Q + G +++ TPV+ V YFP G WYD + ++HS +G W+TL APLD IN+ LR GHI+P+ + + M L + A G+L+WDDGES+ Y+ + F A
Sbjct: 66 APTQCDLPPNS-RFDCAPDKGITPQQCEARGCCYM-PAEWPPDAQ---MGQPWCFFPPSYPSYRLENLTTTETGYTATLTRAVPTFFPKDIMTLRLDMLMETESRLHFTIKDPANRRYEVPLE-TPRVYSQAPFTLYSVEF-SEEPFGVVVRRKLDGRVLLNTTVAPLFFADQFLQLSTSLPSQHITGLAEHLGSLM--LSTNWTKITLWNRDIAPEPNVNLYGSHPFYLVLEDGGLAHGVFLLNSNAMDVVLQPSPALSWRSTGGILDVYIFLGPEPKSVVQQYLDVVGYPFMPPYWGLGFHLCRWGYSTSAITRQVVENMTRAYFPLDVQWNDLDYMDARRDFTFNKDHFGDFPAMVQELHQG-GRRYIMIVDPAISSSGPAGTYRPYDEGLRRGVFITN-ETGQPLIGQVWPGLTAFPDFTNPETLDWWQDMVTEFHAQVPFDGMWIDMNEPSNFVRGSVDGCPDNS-LENPPYLPGVVGGTLRAATICASS-HQFLSTHYDLHNLYGLTEALASHRALVKA-RGMRPFVISRSTFAGHGRYSGHWTGDVWSNWEQLSYSVPEILLFNLLGVPLVGADICGFLGNTSEELCVRWTQLGAFYPFMRNHNALNSQPQEPYRFSETAQQAMRKAFTLRYVLLPYLYTLFHRAHVRGETVARPLFLEFPEDPSTWTVDRQLLWGEALLITPVLEAEKVEVTGYFPQGTWYDLQTVPMEAFGSLPPPAPLTSVIHS--KGQWVTLSAPLDTINVHLRAGHIIPMQGPALTTTESRKQHMALAVALTASGEAQGELFWDDGESLGVLDGGDYTQLIFLA 865
BLAST of Lysosomal alpha-glucosidase vs. TrEMBL
Match: A0A1D1VXK3 (P-type domain-containing protein OS=Ramazzottius varieornatus OX=947166 GN=RvY_16194-1 PE=3 SV=1) HSP 1 Score: 768.844 bits (1984), Expect = 0.000e+0 Identity = 379/859 (44.12%), Postives = 544/859 (63.33%), Query Frame = 3
Query: 312 ATAQCQIANNNERLDCYPESGANQDSCIARGCCWSEPMRLSNKLQGVPLSIPYCYYPTDYSNYMIKSLRKSSRGIKAKLVR--ESPSYLPYEVAMLDLEVIYETKSRTRVRITDPKQKRYEVPMKLSP--PDLKPPTDREYEVIIPSDGSFSIEIKRKSDDRSLFKSF-GPLLYSDQFLQISSIMSTDQIYGIGEQ-NRPFLHQLSNGWERIGLWARDNPIETFMNLYGAHPFYIGMPDDGKAFGVFHFNSNGQEFALQPAPAVTYRTIGGILDFFIFTGGSPEETVKQYLELVGKPMLPPYWSLGFHLSKWGYKNIEQLSEIFQRNINAGFPIGTQWSDIDYMENYKDFTVDKEKFNGLGRFVREIKQKYNSRFVAIVDPGISCTSG-NEYPPYYEGVFMDIFIKDSQTNQPIVGKVWPGE-TVYPDFTHPNISMYWYKQIQRFYNDVPIDGLWIDMNEPSNFYDGSKRGCQGNSKYDNPFFVPDIRDQSLISKTICSSALHYNNEKHYNLHNLYGYTESVTTNKALLKTIHGKRPFILSRSTFSGSGKYTFHWTGDNQASWDQLRNSIRQVLNFNMFGIPMTGADICGFNGNSDEELCIRWMQLGAFYPFMRNHNAINQRDQDPASWNTEAQKIMKTAISNRYAMLPLFYTLLVKSHMNGGTVMRPMSFEFFSDKVTHSIELQFMIGSSIMATPVVYPGARSVDAYFPAGVWYDFHSGKLLHSDAEGAWLTLDAPLDKINIALRGGHIVPLLDSNRNSKSNIPNKMTLYCGMAQDMSASGDLYWDDGESIDSYSRNRYSYVSFNASPEQLLITPVYFGYDLLPVLNKIVILGFENKSITKFLLN-DNDIPFHYNPET 2861
+ A C +++R DC P+ ++ SC+ARGCCW++ + V L +P C+YP Y Y +++ ++++ G + L R ++ P + L L+VI++T R V+ITD + RYEVP+ + P P KP T Y+ ++ + FS ++KRKS L + + DQF+QISS++ + IYG+GE + FLH ++ W++ +W D LYG HPFY M +DG A GV FNSN + LQP PA+TYRTIGG+LDF+ F G SP++ QY+EL+GKP +PPYW+LG+HL +WGY ++E ++ +RN+ AG P+ QW+DIDYM+ KDFT+D KFNG+ FV E+ K R+V IVDPGIS Y Y +G+ M++F++++ ++Q I G+VW G YPDFTHPNIS YW KQI F VP+DGLWIDMNEPSNF DGS GC +S +NP ++P+I ++L ++T+C +A H+ HYNLH+ YG ++ T ALL+ KRPF++SRSTF G G+Y+ HWTGD +SW L+ SI +LNFNMFGIP GADICGF N+ EELC+RW QLGAFYPF RNHNA++ RDQDPA+W+ A + ++T + RY++LP + TLL ++ ++G TV+RP+ FEF DK T+SI+ QF+IGSS+M +PV P + AY P G W+DF+S K + SD G W+ LDAPLDK+N+ LR G IVP+ + N + L + + A G++YWDDGE +D + +S V F +L T V Y +P + + ++G E +++ K + N ++ F +N T
Sbjct: 186 SNAVCSTMADDDRFDCLPQGDISEASCMARGCCWNQRSSVQMVNGTVKLGLPACFYPYKYPTYRLENYKETANGFEGTLQRSKDAKDIYPNPINSLRLQVIFDTAQRLHVKITDATKDRYEVPLPVLPGAPFSKPDTTL-YDFVVTKE-PFSFQVKRKSTGGVLVDTATNTFAFYDQFIQISSLLPSKYIYGLGEHTDAHFLHSVN--WKQFTMWTTDIYPNLETPLYGVHPFYFSMEEDGDANGVLLFNSNAMDVILQPTPAITYRTIGGVLDFYFFLGPSPQDVTSQYMELLGKPQMPPYWALGYHLCRWGYGSVENTRKVMERNLAAGIPLDAQWNDIDYMDKNKDFTIDGSKFNGITEFVNEVHAK-GLRYVVIVDPGISSAEAPGSYQAYTDGLAMNVFVQNA-SDQVIEGRVWTGNPQAYPDFTHPNISQYWLKQITDFRKVVPVDGLWIDMNEPSNFVDGSMVGCPKDSSLENPPYLPNIAGKTLNTRTLCMTAKHHAG-LHYNLHSTYGIYQAKATQAALLQVFPNKRPFVVSRSTFVGQGQYSAHWTGDESSSWSDLQTSIGDILNFNMFGIPFVGADICGFGDNATEELCLRWHQLGAFYPFSRNHNAVSNRDQDPAAWSPAATEAIRTVLLTRYSLLPYYSTLLFRASISGHTVVRPLFFEFAHDKRTYSIDAQFLIGSSVMLSPVTTPNTTKILAYLPEGRWFDFYSLKKI-SDKGGEWIELDAPLDKVNVHLREGGIVPMQKPEVTTGKTRQNPIDLVVMLDKTGKACGEVYWDDGEQMDVLQKGDFSLVQFEMKNGKLKSTVVNDKYKDVPAMGSVRVVGLE-RTVKKVVWNGGEEVDFVFNDHT 1035
BLAST of Lysosomal alpha-glucosidase vs. TrEMBL
Match: A0A4Z2D8T0 (Lysosomal alpha-glucosidase OS=Schistosoma japonicum OX=6182 GN=EWB00_003283 PE=3 SV=1) HSP 1 Score: 755.747 bits (1950), Expect = 0.000e+0 Identity = 382/871 (43.86%), Postives = 537/871 (61.65%), Query Frame = 3
Query: 312 ATAQCQIANNNERLDCYPESGANQDSCIARGCCWSEPMRLSNKLQGVPLSIPYCYYPTDYSNYMIKSLRKSSRGIKAKLVRESPSYLPYEVAMLDLEVIYETKSRTRVRITDPKQ-KRYEVPMKLSPPDLKPPTDREYEVIIPSDGSFSIEIKRKSDDRS-LFKSFGPL----LYSDQFLQISSIMSTDQIYGIGEQNRPFLHQLSNGWERIGLWARDNPIETFMNLYGAHPFYIGMPDDGKAFGVFHFNSNGQEFALQPAPAVTYRTIGGILDFFIFTGGSPEETVKQYLELVGKPMLPPYWSLGFHLSKWGYKNIEQLSEIFQRNINAGFPIGTQWSDIDYMENYKDFTVDKEKFNGLGRFVREIKQK-YNSRFVAIVDPGISCTSGNEYPPYYEGVFMDIFIKDSQTNQPIVGKVWPGETVYPDFTHPNISMYWYKQIQRFYNDVPIDGLWIDMNEPSNFYDGSKRGCQGNSKYDNPFFVPDIRDQSLISKTICSSALHYNNEKHYNLHNLYGYTESVTTNKALLKTIHGKRPFILSRSTFSGSGKYTFHWTGDNQASWDQLRNSIRQVLNFNMFGIPMTGADICGFNGNSDEELCIRWMQLGAFYPFMRNHNAINQRDQDPASWNTEAQKIMKTAISNRYAMLPLFYTLLVKSHMNGGTVMRPMSFEFFSDKVTHSIELQFMIGSSIMATPVVYPGARSVDAYFPAGVWYDFHSGKLLHSDAEGAWLTLDAPLDKINIALRGGHIVPLLDSNRNSKSNIPNKMTLYCGM----------AQDMSASGDLYWDDGESIDSYSRNRYSYVSFNASPEQLLITPVYFGYDLLPVLN-------KIVILGFENKSITKFLLNDNDIPFHYN 2852
+ A C N +RLDCYP G+N+ CI RGCCW +N+ G +PYCY+P DYS Y + S +++ G A+L + +P+Y E+ + +E+ ET +R R+R T P Q R+E ++L PD P + +Y+V + F ++I R + ++ L S G L + SDQFLQIS ++ + +G GE + + + W R+GLW D ++ NLYG H F++G+ DG AFGVF NSN QE A+ P PA+TYRTIGGILDFF+FTG P + + QY L+G P LPPYWSLGFHL ++G KN+ ++ + +RN+ AG PI QW DIDYM+ YK ++VDK++F+G+G +V +I K Y+ R V IVDP +S G+ Y PY +G+ + +FI DS+T PI+G VWPGETV+PDF+HP+ +WYK FY V DGLWIDMNEP+NF GS GC +K DNP ++P I SL KTIC SALHYN HYNLHN+YGY E+ T+ L + +RPFIL+RS+++GSG Y HWTGD ++WD L+ S+ Q++NFNMFGIPM GADICGF GN+ +ELC+RW QLGAFYPF RNHN DQDPA W+ + + +K ++ RY +LP YTL ++++NG TV R ++FEF D TH I QFM+GS I+ TPV+ G V+ Y P+G W + +GK S G W+ DAPL+ I I+ R G I+P+ + + L+ + + ++ASG+L+WD+G+ ++ Y Y+ F +L + D ++ ++I+GF K + +FLLND IPF Y+
Sbjct: 21 SNAHCSDITNVQRLDCYPNVGSNESGCIERGCCWMP----NNEQDG----LPYCYFPKDYSAYTVVSTQQTKNGFIAQLSKLNPTYYSDEIKNIAVEMREETSTRLRLRFTAPSQPNRWEPSIQLGNPDNIPIENVQYKVSM-EKSPFGLKIMRNTKEQEVLLDSTGSLSSSFIVSDQFLQISFRVNAETSFGPGEVEQKYPNDFET-WTRLGLWGHDGDPHSYANLYGTHNFFMGLKHDGTAFGVFFLNSNAQELAITPLPAITYRTIGGILDFFVFTGPKPLDVLNQYYTLIGHPTLPPYWSLGFHLCRYGMKNLSEVRSVLKRNVEAGIPIDAQWFDIDYMDAYKIWSVDKKRFDGIGTYVNDILHKQYSMRTVIIVDPAVSTKGGSGYLPYEDGMRLGVFINDSRTGTPIIGTVWPGETVFPDFSHPSTEDWWYKSASDFYEVVNFDGLWIDMNEPANFNGGSLTGCPSWNKLDNPPYIPKIIQNSLYDKTICPSALHYNT-THYNLHNMYGYHEARVTHNVLKRLFPDRRPFILTRSSYAGSGMYAAHWTGDVLSNWDSLKASVAQIINFNMFGIPMVGADICGFVGNTTQELCVRWSQLGAFYPFSRNHNEDEASDQDPAYWSKDTIEAIKESLELRYHLLPYIYTLFYRAYLNGTTVARALAFEFPEDLSTHKINAQFMLGSCILVTPVLDEGRVGVEGYVPSGEWINLSTGKRYFS--RGTWMYFDAPLNIIPISTRCGCIIPIQIAAETTDIARKKGFGLFVILSSSDDGSDASGERITASGELFWDNGDD----AKLNYVYIKFEVRNRKLSVISTPSSVDTFQKIDLNELEVKTVLIVGFLRKPL-EFLLNDKAIPFMYD 873
BLAST of Lysosomal alpha-glucosidase vs. TrEMBL
Match: H2KPA9 (Lysosomal alpha-glucosidase OS=Clonorchis sinensis OX=79923 GN=CLF_101613 PE=3 SV=1) HSP 1 Score: 748.043 bits (1930), Expect = 0.000e+0 Identity = 386/869 (44.42%), Postives = 524/869 (60.30%), Query Frame = 3
Query: 321 QCQIANNNERLDCYPESGANQDSCIARGCCWSEPMRLSNKLQGVPLS---IPYCYYPTDYSNYMIKSLRKSSRGIKAKLVRESPSYLPYEVAMLDLEVIYETKSRTRVRITDPKQ-KRYEVPMKLSPPDLKPPTDREYEVIIPSDGSFSIEIKRKSDDRS-LFKSFGPL----LYSDQFLQISSIMSTDQIYGIGEQNRPFLHQLSNGWERIGLWARDNPIETFMNLYGAHPFYIGMPDDGKAFGVFHFNSNGQEFALQPAPAVTYRTIGGILDFFIFTGGSPEETVKQYLELVGKPMLPPYWSLGFHLSKWGYKNIEQLSEIFQRNINAGFPIGTQWSDIDYMENYKDFTVDKEKFNGLGRFVRE-IKQKYNSRFVAIVDPGISCTSGNEYPPYYEGVFMDIFIKDSQTNQPIVGKVWPGETVYPDFTHPNISMYWYKQIQRFYNDVPIDGLWIDMNEPSNFYDGSKRGCQGNSKYDNPFFVPDIRDQSLISKTICSSALHYNNEKHYNLHNLYGYTESVTTNKALLKTIHGKRPFILSRSTFSGSGKYTFHWTGDNQASWDQLRNSIRQVLNFNMFGIPMTGADICGFNGNSDEELCIRWMQLGAFYPFMRNHNAINQRDQDPASWNTEAQKIMKTAISNRYAMLPLFYTLLVKSHMNGGTVMRPMSFEFFSDKVTHSIELQFMIGSSIMATPVVYPGARSVDAYFPAGVWYDFHSGKLLHSDAEGAWLTLDAPLDKINIALRGGHIVPLLDSNRNSKSNIPNKMTLYCGMA---------QDMSASGDLYWDDGESIDSYSRNRYSYVSFNASPEQLLITPVYFGYDLLPVLN------KIVILGFENKSITKFLLNDNDIPFHYN 2852
QC +ERLDCYPE+GAN+D+CI +GCCWS G+ + IP CY+P DY Y ++++ ++ RG A + + + Y E + +EV++ETK+R RVR T P R+E P+ L PD+ P +Y V + F I + R R L S G + ++SDQFLQ S + + +G GE F H L W R+GLW RDN NLYG H F++G+ DG AFGVF NSN QE L P PA+TYRTIGGILDFFIFTG P + QYL L+G P+LPPYWSLGFHL ++GY++ E+++ +RNI AG P+ QW DIDYM+ Y+ +TVD KF GL +++ + KY R V IVDP +S Y P+ +G IFI DS+T PI G VWPG+TV+PDF+HP + Y F+ VP DGLWIDMNEP++F GSK C + DNP FVP I D+SL +KT+C SALH+++ HYN HNLYGY + T+ L + GKRPF+LSRSTFSGSG YT HWTGDN +SW +R SI Q++NFN+FGIPM GADICGF GN+ EELC+RW QLGAFYPF RNHN + QDPA W+ A + ++ AI RY +LP Y+L ++H+NG TV R +SFEF D TH+++ QFM+GS I+ TPV+ G V Y PAG W + +G+ +S + + APL+ I I +RGG +VP S +K + + L ++ Q +A G+L+WDDGE+ Y YV+F + +L I P D + ++ K +++ ++ + LLN + + ++
Sbjct: 28 QCGPFPISERLDCYPEAGANKDACINKGCCWS----------GITFTEPHIPMCYFPKDYRTYTVRNVTQNPRGFVADMEKITSPYGVNEFQNVRVEVLHETKTRLRVRFTIPSDPNRWEPPIPLGKPDVNAPEPTDYAVEL-EKSPFGIRVVRNDPIRQMLLDSTGDMSVSTIFSDQFLQTSFRLGAEHGFGPGEVRADFPHWLRRTWLRMGLWTRDNAPMKEANLYGVHNFFMGLSPDGSAFGVFLINSNAQEVVLTPLPAITYRTIGGILDFFIFTGPDPMDVTAQYLSLIGHPLLPPYWSLGFHLCRFGYRDSEEVANTVERNIKAGIPVDAQWIDIDYMDAYRVWTVDPTKFGGLPSLIQDTLHSKYGIRNVLIVDPAVSAKDDPTYAPFLDGFKRGIFINDSRTGSPIEGVVWPGDTVFPDFSHPEAEAWLYNLASEFHKSVPFDGLWIDMNEPASFAAGSKTNCDQTNPLDNPPFVPPILDRSLYAKTVCPSALHHDS-THYNRHNLYGYDHARVTHNVLKQLFAGKRPFLLSRSTFSGSGLYTIHWTGDNLSSWPDMRASIAQIINFNLFGIPMVGADICGFRGNTTEELCVRWSQLGAFYPFARNHNDLASIPQDPAVWSKAATEAIRDAIKLRYLLLPYMYSLFYRAHLNGTTVARALSFEFPRDTNTHTVKEQFMLGSCILVTPVLDEGRTDVTGYVPAGYWINLSTGRRDYSTGQVKY--FQAPLNVIPILVRGGCVVPFQTSFEVTKISRKKGIALLVVLSTTDDGTLIPQTGTAKGELFWDDGEA----DPLNYVYVNFTVANRELHIVPKPLNADTIRSIDPQETQIKSILINGLSQEPKQVLLNKAPVAYTFD 878
BLAST of Lysosomal alpha-glucosidase vs. TrEMBL
Match: A0A419PZA5 (Lysosomal alpha-glucosidase OS=Clonorchis sinensis OX=79923 GN=Gaa PE=3 SV=1) HSP 1 Score: 747.273 bits (1928), Expect = 0.000e+0 Identity = 385/869 (44.30%), Postives = 524/869 (60.30%), Query Frame = 3
Query: 321 QCQIANNNERLDCYPESGANQDSCIARGCCWSEPMRLSNKLQGVPLS---IPYCYYPTDYSNYMIKSLRKSSRGIKAKLVRESPSYLPYEVAMLDLEVIYETKSRTRVRITDPKQ-KRYEVPMKLSPPDLKPPTDREYEVIIPSDGSFSIEIKRKSDDRS-LFKSFGPL----LYSDQFLQISSIMSTDQIYGIGEQNRPFLHQLSNGWERIGLWARDNPIETFMNLYGAHPFYIGMPDDGKAFGVFHFNSNGQEFALQPAPAVTYRTIGGILDFFIFTGGSPEETVKQYLELVGKPMLPPYWSLGFHLSKWGYKNIEQLSEIFQRNINAGFPIGTQWSDIDYMENYKDFTVDKEKFNGLGRFVRE-IKQKYNSRFVAIVDPGISCTSGNEYPPYYEGVFMDIFIKDSQTNQPIVGKVWPGETVYPDFTHPNISMYWYKQIQRFYNDVPIDGLWIDMNEPSNFYDGSKRGCQGNSKYDNPFFVPDIRDQSLISKTICSSALHYNNEKHYNLHNLYGYTESVTTNKALLKTIHGKRPFILSRSTFSGSGKYTFHWTGDNQASWDQLRNSIRQVLNFNMFGIPMTGADICGFNGNSDEELCIRWMQLGAFYPFMRNHNAINQRDQDPASWNTEAQKIMKTAISNRYAMLPLFYTLLVKSHMNGGTVMRPMSFEFFSDKVTHSIELQFMIGSSIMATPVVYPGARSVDAYFPAGVWYDFHSGKLLHSDAEGAWLTLDAPLDKINIALRGGHIVPLLDSNRNSKSNIPNKMTLYCGMA---------QDMSASGDLYWDDGESIDSYSRNRYSYVSFNASPEQLLITPVYFGYDLLPVLN------KIVILGFENKSITKFLLNDNDIPFHYN 2852
QC +ERLDCYPE+GAN+D+CI +GCCWS G+ + IP CY+P DY Y ++++ ++ RG A + + + Y E + +EV++ETK+R RVR T P R+E P+ L PD+ P +Y V + F I + R R L S G + ++SDQFLQ S + + +G GE F H L W R+GLW RDN NLYG H F++G+ DG AFGVF NSN QE L P PA+TYRTIGGILDFFIFTG P + QYL L+G P+LPPYWSLGFHL ++GY++ E+++ +RNI AG P+ QW DIDYM+ Y+ +TVD KF GL +++ + KY R V IVDP +S Y P+ +G IFI DS+T PI G VWPG+TV+PDF+HP + Y F+ VP DGLWIDMNEP++F GSK C + DNP FVP I D+SL +KT+C SALH+++ HYN HNLYGY + T+ L + GKRPF+LSRSTFSGSG YT HWTGDN +SW +R SI Q++NFN+FGIPM GADICGF GN+ EELC+RW QLGAFYPF RNHN + QDPA W+ A + ++ AI RY +LP Y+L ++H+NG TV R ++FEF D TH+++ QFM+GS I+ TPV+ G V Y PAG W + +G+ +S + + APL+ I I +RGG +VP S +K + + L ++ Q +A G+L+WDDGE+ Y YV+F + +L I P D + ++ K +++ ++ + LLN + + ++
Sbjct: 28 QCGPFPISERLDCYPEAGANKDACINKGCCWS----------GITFTEPHIPMCYFPKDYRTYTVRNVTQNPRGFVADMEKITSPYGVNEFQNVRVEVLHETKTRLRVRFTIPSDPNRWEPPIPLGKPDVNAPEPTDYAVEL-EKSPFGIRVVRNDPIRQMLLDSTGDMSVSTIFSDQFLQTSFRLGAEHGFGPGEVRADFPHWLRRTWLRMGLWTRDNAPMKEANLYGVHNFFMGLSPDGSAFGVFLINSNAQEVVLTPLPAITYRTIGGILDFFIFTGPDPMDVTAQYLSLIGHPLLPPYWSLGFHLCRFGYRDSEEVANTVERNIKAGIPVDAQWIDIDYMDAYRVWTVDPTKFGGLPSLIQDTLHSKYGIRNVLIVDPAVSAKDDPTYAPFLDGFKRGIFINDSRTGSPIEGVVWPGDTVFPDFSHPEAEAWLYNLASEFHKSVPFDGLWIDMNEPASFAAGSKTNCDQTNPLDNPPFVPPILDRSLYAKTVCPSALHHDS-THYNRHNLYGYDHARVTHNVLKQLFAGKRPFLLSRSTFSGSGLYTIHWTGDNLSSWPDMRASIAQIINFNLFGIPMVGADICGFRGNTTEELCVRWSQLGAFYPFARNHNDLASIPQDPAVWSKAATEAIRDAIKLRYLLLPYMYSLFYRAHLNGTTVARALAFEFPRDTNTHTVKEQFMLGSCILVTPVLDEGRTDVTGYVPAGYWINLSTGRRDYSTGQVKY--FQAPLNVIPILVRGGCVVPFQTSFEVTKVSRKKGIALLVVLSTTDDGTLIPQTGTAKGELFWDDGEA----DPLNYVYVNFTVANRELHIVPKPLNADTIRSIDPQETQIKSILINGLSQEPKQVLLNKAPVAYTFD 878
BLAST of Lysosomal alpha-glucosidase vs. TrEMBL
Match: A0A4S2MAN8 (P-type domain-containing protein OS=Opisthorchis felineus OX=147828 GN=CRM22_002390 PE=3 SV=1) HSP 1 Score: 743.421 bits (1918), Expect = 0.000e+0 Identity = 378/821 (46.04%), Postives = 505/821 (61.51%), Query Frame = 3
Query: 321 QCQIANNNERLDCYPESGANQDSCIARGCCWSEPMRLSNKLQGVPLSIPYCYYPTDYSNYMIKSLRKSSRGIKAKLVRESPSYLPYEVAMLDLEVIYETKSRTRVRITDPKQ-KRYEVPMKLSPPDLKPPTDREYEVIIPSDGSFSIEIKRKSDDRS-LFKSFGPL----LYSDQFLQISSIMSTDQIYGIGEQNRPFLHQLSNGWERIGLWARDNPIETFMNLYGAHPFYIGMPDDGKAFGVFHFNSNGQEFALQPAPAVTYRTIGGILDFFIFTGGSPEETVKQYLELVGKPMLPPYWSLGFHLSKWGYKNIEQLSEIFQRNINAGFPIGTQWSDIDYMENYKDFTVDKEKFNGLGRFVRE-IKQKYNSRFVAIVDPGISCTSGNEYPPYYEGVFMDIFIKDSQTNQPIVGKVWPGETVYPDFTHPNISMYWYKQIQRFYNDVPIDGLWIDMNEPSNFYDGSKRGCQGNSKYDNPFFVPDIRDQSLISKTICSSALHYNNEKHYNLHNLYGYTESVTTNKALLKTIHGKRPFILSRSTFSGSGKYTFHWTGDNQASWDQLRNSIRQVLNFNMFGIPMTGADICGFNGNSDEELCIRWMQLGAFYPFMRNHNAINQRDQDPASWNTEAQKIMKTAISNRYAMLPLFYTLLVKSHMNGGTVMRPMSFEFFSDKVTHSIELQFMIGSSIMATPVVYPGARSVDAYFPAGVWYDFHSGKLLHSDAEGAWLTLDAPLDKINIALRGGHIVPLLDSNRNSKSNIPNKMTLYCGMA---------QDMSASGDLYWDDGESIDSYSRNRYSYVSFNASPEQLLITP 2735
QC +ERLDCYPE GAN+D+CI +GCCWS + IP CY+P DY Y ++++ ++ RG A + + + Y E + +EV++ETK+R RVR P R+E P+ L PD+ P +Y V + F I + R R L S G L ++SDQFLQ S + + +G GE F H L W R+GLWARDN NLYG H F++G+ DG AFGVF NSN QE L P PA+TYR IGGILDFFIFTG P + QYL L+G P+LPPYWSLGFHL ++GY++ E++++ +RN+ AG P+ QW DIDYM+ Y+ +TVD KF GL +++ + KY R V IVDP +S Y P+ +G+ IFI DS+T PI G VWPG+TV+PDF+HP + Y +F+ VP DGLWIDMNEP++F GSK C + +DNP FVP I D+SL +KT+C SALH+++ HYN HNLYGY + T+ L + GKRPF+LSRSTFSGSG YT HWTGDN +SW +R SI Q++NFN+FGIPM GADICGF GN+ EELC+RW QLGAFYPF RNHN + QDPA W+ A + ++ AI RY +LP Y+L ++H+NG TV R ++FEF D TH++ QFM+GS I+ TPV+ G V Y PAG W + +G+ +S + + APL+ I I +RGG IVP S +K + + L ++ Q +A G+L+WDDGE+ Y +V+F + +L I P
Sbjct: 28 QCGPFPISERLDCYPEEGANKDACINKGCCWS-------GIDFTEPHIPMCYFPKDYRTYTVRNVTQNPRGFVADMEKITSLYGVNEFQNVRVEVLHETKTRLRVRFAIPSDPNRWEPPIPLGKPDVDAPEPTDYAVEL-EKSPFGIRVVRNDPTRQMLLDSTGDLSVSTIFSDQFLQTSFRLGAEHGFGPGEVRADFPHWLRRTWIRMGLWARDNAPVKEANLYGVHNFFMGLSPDGSAFGVFLLNSNAQEVVLTPLPAITYRAIGGILDFFIFTGPEPIDVTAQYLSLIGHPLLPPYWSLGFHLCRYGYRDSEEVADTVERNVKAGIPLDAQWIDIDYMDAYRIWTVDSTKFGGLPSLIQDTLHSKYGIRNVLIVDPAVSAKDDPTYAPFQDGLKRGIFINDSRTGSPIEGVVWPGDTVFPDFSHPEAEAWVYNFASQFHKSVPFDGLWIDMNEPASFAAGSKTNCDQTNPFDNPPFVPLILDRSLYAKTVCPSALHHDS-THYNRHNLYGYDHARVTHNVLKQLFAGKRPFLLSRSTFSGSGLYTIHWTGDNLSSWPDMRASIAQIINFNLFGIPMVGADICGFRGNTTEELCVRWSQLGAFYPFSRNHNDLASIPQDPAVWSKTATEAIRDAIRLRYLLLPYMYSLFYRAHLNGTTVARALAFEFPRDTNTHTVGEQFMLGSCILVTPVLDEGRTDVTGYVPAGYWINLSTGRRDYSTGQVKY--FQAPLNVIPILVRGGCIVPFQTSFEVTKVSRKKGIALLVVLSTTDDGTSVPQTGTAKGELFWDDGEA----DPLNYVHVNFTVANRELHIVP 833
BLAST of Lysosomal alpha-glucosidase vs. Ensembl Cavefish
Match: si:ch73-12o23.1 (lysosomal alpha-glucosidase [Source:NCBI gene;Acc:103033787]) HSP 1 Score: 687.952 bits (1774), Expect = 0.000e+0 Identity = 342/797 (42.91%), Postives = 485/797 (60.85%), Query Frame = 3
Query: 324 CQIANNNERLDCYPESGA--NQDSCIARGCCWSEPMRLSNKLQGVPLSIPYCYYPTDYSNYMIKSLRKSSRGIKAKLVRESPSYLPYEVAMLDLEVIYETKSRTRVRITDPKQKRYEVPMKLSPPDLKPPTDREYEVIIPSDGSFSIEIKRKSDDRSLFKS-FGPLLYSDQFLQISSIMSTDQIYGIGEQNRPFLHQLSNGWERIGLWARDNPIETFMNLYGAHPFYIGMPDDGKAFGVFHFNSNGQEFALQPAPAVTYRTIGGILDFFIFTGGSPEETVKQYLELVGKPMLPPYWSLGFHLSKWGYKNIEQLSEIFQRNINAGFPIGTQWSDIDYMENYKDFTVDKEKFNGLGRFVREIKQKYNSRFVAIVDPGISCTS-GNEYPPYYEGVFMDIFIKDSQTNQPIVGKVWPGETVYPDFTHPNISMYWYKQIQRFYNDVPIDGLWIDMNEPSNFYDGSKRGCQGNSKYDNPFFVPDIRDQSLISKTICSSALHYNNEKHYNLHNLYGYTESVTTNKALLKTIHGKRPFILSRSTFSGSGKYTFHWTGDNQASWDQLRNSIRQVLNFNMFGIPMTGADICGFNGNSDEELCIRWMQLGAFYPFMRNHNAINQRDQDPASWNTEAQKIMKTAISNRYAMLPLFYTLLVKSHMNGGTVMRPMSFEFFSDKVTHSIELQFMIGSSIMATPVVYPGARSVDAYFPAGVWYDFHSGKLLHSDAEGAWLTLDAPLDKINIALRGGHIVPLLDSNRNSKSNIPNKMTLYCGMAQDMSASGDLYWDDGESIDSYSRNRYSYVSF 2702
C + R DCYPE G N++ C R CC+ + + Q +P+C+YP D+ +Y + + ++ G + KLVR +Y P ++ L L+V+YE+ SR R+R+TDP + RYEVP+ + P +K + ++ F + +KRK + L + PL Y+DQFLQ+S+ ++++ +YG+GE FLH + W + +W RD NLYG HPFY+ M DG A G F NSN + LQPAPA+T+RTIGGILD ++F G P V +YLE+VGKP +P YW+LG+HL +WGY + ++ ++ N P QW+DIDYM+ DFT D F L V+++ ++ +V I+DPGIS T Y P+ EG+ +FI +S + ++GKVWPG T +PDF++P+ +WY+ ++ F++ VP DGLWIDMNEPSNF DGS GC N +NP + P + SL +KT+C+SA N HYNLHNLYG E+ + AL K + KRPF++SRSTFS G Y+ HW GDN++ W L SI +L FN+ GIP+ GADICGF+ + EELC+RW QLGAFYPF RNHN + + QDP ++ A+K M + RY++ P YTL +H++G TV RP+ FEF D T+ I+ QF+ G S++ TPV+ PG V Y P G+WYDF++G L S E + L APLDKIN+ LR G I P N + + L + +D A GDL+WDDGE+ID+Y ++Y+Y F
Sbjct: 103 CTLIPEGWRFDCYPERGVVVNEEMCHKRNCCFIQVAEEKSPGQN---GVPWCFYPPDFPSYELVTNNETEMGYRGKLVRRQKTYYPNDIETLQLDVLYESDSRLRIRVTDPAKDRYEVPIDV--PVVKKKASNPLYTVEFTEQPFGLIVKRKQNGAVLLNTTVAPLFYADQFLQMSTSLASEFVYGLGEHRSNFLHDVQ--WNTLTMWTRDAAPVELTNLYGVHPFYLLMESDGLAHGSFLLNSNAMDVVLQPAPALTWRTIGGILDLYVFVGPDPSSVVGEYLEVVGKPAMPIYWALGYHLCRWGYDSSNNTWDVVKKMRNNQIPQDVQWNDIDYMDRALDFTHDPVNFATLPDLVKDL-HIHDQHYVMILDPGISSTQPAGSYLPFQEGIKQGVFITNSSGDL-LIGKVWPGLTAFPDFSNPDTHEWWYQNLKTFHDQVPFDGLWIDMNEPSNFLDGSVDGCPSND-LENPPYTPAVLGGSLRAKTVCASARQKLN-SHYNLHNLYGLMEAKASASALKKLLK-KRPFVISRSTFSSHGVYSGHWLGDNRSQWKDLYMSIPGMLTFNILGIPLVGADICGFSATTTEELCVRWTQLGAFYPFTRNHNTKDSQAQDPTVFSPAARKAMTEVLLLRYSLFPFLYTLFHHAHLSGHTVARPLFFEFPRDLKTYKIDKQFLWGKSLLVTPVLAPGVDYVVGYLPQGIWYDFYTGDSLVSKGEE--IKLHAPLDKINLHLREGSITPTQRPNTTLWVSSSQPLHLIACLTEDGLAEGDLFWDDGETIDTYETDQYAYFIF 885
BLAST of Lysosomal alpha-glucosidase vs. Ensembl Cavefish
Match: gaa (glucosidase alpha, acid [Source:NCBI gene;Acc:103044115]) HSP 1 Score: 676.396 bits (1744), Expect = 0.000e+0 Identity = 353/833 (42.38%), Postives = 495/833 (59.42%), Query Frame = 3
Query: 312 ATAQCQIANNNERLDCYPESGANQDSCIARGCCWSEPMRLSNKLQGVPLSIPYCYYPTDYSNYMIKSLRKSSRGIKAKLVRESPSYLPYEVAMLDLEVIYETKSRTRVRITDPKQKRYEVPMKLSPPDLKPPTDRE---YEVIIPSDGSFSIEIKRKSDDRSLF-KSFGPLLYSDQFLQISSIMSTDQIYGIGEQNRPFLHQLSNGWERIGLWARDNPIETFMNLYGAHPFYIGMPDDGKAFGVFHFNSNGQEFALQPAPAVTYRTIGGILDFFIFTGGSPEETVKQYLELVGKPMLPPYWSLGFHLSKWGYKNIEQLSEIFQRNINAGFPIGTQWSDIDYMENYKDFTVDKEKFNGLGRFVREIKQKYNSRFVAIVDPGISCTSG-NEYPPYYEGVFMDIFIKDSQTNQPIVGKVWPGETVYPDFTHPNISMYWYKQIQRFYNDVPIDGLWIDMNEPSNFYDGSKRGCQGNSKYDNPFFVPDIRDQSLISKTICSSALHYNNEKHYNLHNLYGYTESVTTNKALLKTIHGKRPFILSRSTFSGSGKYTFHWTGDNQASWDQLRNSIRQVLNFNMFGIPMTGADICGFNGNSDEELCIRWMQLGAFYPFMRNHNAINQRDQDPASWNTEAQKIMKTAISNRYAMLPLFYTLLVKSHMNGGTVMRPMSFEFFSDKVTHSIELQFMIGSSIMATPVVYPGARSVDAYFPAGVWYDFHSGKLLHSDAEGAWLTLDAPLDKINIALRGGHIVPLLDSNRNSKSNIPNKMTLYCGMAQDMSASGDLYWDDGESIDSYSRNRYSYVSFNASPEQLLITP--VYFGYDLLPVLNKIVILG 2789
A C IA R DC + Q C RGCC+ + L P P+C+YP Y Y + L +SRG +A L R + SYLP +V L LEV+ E R + + DP Q RYEVP PP PTD + Y+V D F ++R S+ R L + PLL++DQ+LQ+S+ +++ + G GE L+ W + LW RD NLYG+HPF + DG+A+G+F NSN E QPAPA+T+ T GGI+DF+IF G P+ +QY +++G PM+PPYWSLGFHL +WGY + + Q A FP+ QW+D+DY + + FT D ++F L V+E + ++V I+DPGIS TS Y P+ EG +FI++ T Q ++GKVWPG T +PDFT+P+ +W + I FY VP+DGLWIDMNEP+NF GS GC ++ +NPF+VPD+ L + T+C S+ + HYNLHNLYG TE++ T+ ALLK G RPF+LSRS+F G G+Y+ HWTGD + W+QLR SI VL F ++G+P+ GADICGF G++ EELC+RW QLGAFYPFMRNHN + Q+P + +AQ M++ + RY++LP YTL ++ + TV RP+ EF D +I+ QF+ GSS++ +PV+ GA + AY P G+WY + G+ +S G +L L APLD IN+ +RGG I+P + ++ N L ++ D A G+L+WDDGES+ ++ R YS++ F A QL+ P V D L VL +I + G
Sbjct: 77 AGKDCGIAVE-RRFDCARDRALTQSQCEQRGCCY---VPLPKSAFSGP---PWCFYPVSYPGYRMGPLVPTSRGQEANLTRSTSSYLPRDVQTLTLEVMAEEAGRLHIALKDPSQDRYEVPFVAVPPTT--PTDTQSFLYDVEFQPD-PFGFVVRRASNGRVLLNTTVAPLLFADQYLQLSTTLASSLVSGFGEHYTGLTLDLN--WTSVTLWNRDMAPHRDTNLYGSHPFCLVQEGDGQAYGIFLLNSNAMEVVSQPAPALTWVTTGGIMDFYIFLGPDPQSVAQQYHQVIGYPMMPPYWSLGFHLCRWGYTSTNATRAVVQHMHQANFPLDVQWNDLDYADKRRVFTFDPQRFGDLPEMVKEFHSE-GMKYVLILDPGISSTSPPGSYKPFDEGERRGVFIRN-LTGQTLIGKVWPGPTAFPDFTNPDAVSWWEECIADFYAKVPVDGLWIDMNEPANFVQGSLLGCP-DTDLENPFYVPDMIGGQLNAGTLCMSS-QQSLSAHYNLHNLYGLTEAMATHSALLKA-RGSRPFVLSRSSFPGLGRYSGHWTGDVHSDWEQLRYSIPAVLLFGLYGVPLVGADICGFEGDTSEELCVRWTQLGAFYPFMRNHNDRSNAPQEPYVFGQQAQAAMRSVMMLRYSLLPFLYTLFHQAQTSASTVARPLFLEFPFDPNCQTIDQQFLWGSSLLISPVLEQGAVELTAYLPPGMWYSLYDGQPYNS--RGQYLLLSAPLDTINVHVRGGRIIPQQMPALTTTASRSNPFLLTVALSSDGWAVGELFWDDGESLGTFERGDYSFIIFTAEQSQLVSEPGKVNGALDGL-VLGEIRVFG 889
BLAST of Lysosomal alpha-glucosidase vs. Ensembl Cavefish
Match: gaa (glucosidase alpha, acid [Source:NCBI gene;Acc:103044115]) HSP 1 Score: 673.315 bits (1736), Expect = 0.000e+0 Identity = 346/810 (42.72%), Postives = 486/810 (60.00%), Query Frame = 3
Query: 312 ATAQCQIANNNERLDCYPESGANQDSCIARGCCWSEPMRLSNKLQGVPLSIPYCYYPTDYSNYMIKSLRKSSRGIKAKLVRESPSYLPYEVAMLDLEVIYETKSRTRVRITDPKQKRYEVPMKLSPPDLKPPTDRE---YEVIIPSDGSFSIEIKRKSDDRSLF-KSFGPLLYSDQFLQISSIMSTDQIYGIGEQNRPFLHQLSNGWERIGLWARDNPIETFMNLYGAHPFYIGMPDDGKAFGVFHFNSNGQEFALQPAPAVTYRTIGGILDFFIFTGGSPEETVKQYLELVGKPMLPPYWSLGFHLSKWGYKNIEQLSEIFQRNINAGFPIGTQWSDIDYMENYKDFTVDKEKFNGLGRFVREIKQKYNSRFVAIVDPGISCTSG-NEYPPYYEGVFMDIFIKDSQTNQPIVGKVWPGETVYPDFTHPNISMYWYKQIQRFYNDVPIDGLWIDMNEPSNFYDGSKRGCQGNSKYDNPFFVPDIRDQSLISKTICSSALHYNNEKHYNLHNLYGYTESVTTNKALLKTIHGKRPFILSRSTFSGSGKYTFHWTGDNQASWDQLRNSIRQVLNFNMFGIPMTGADICGFNGNSDEELCIRWMQLGAFYPFMRNHNAINQRDQDPASWNTEAQKIMKTAISNRYAMLPLFYTLLVKSHMNGGTVMRPMSFEFFSDKVTHSIELQFMIGSSIMATPVVYPGARSVDAYFPAGVWYDFHSGKLLHSDAEGAWLTLDAPLDKINIALRGGHIVPLLDSNRNSKSNIPNKMTLYCGMAQDMSASGDLYWDDGESIDSYSRNRYSYVSFNASPEQLL 2726
A C IA R DC + Q C RGCC+ + L P P+C+YP Y Y + L +SRG +A L R + SYLP +V L LEV+ E R + + DP Q RYEVP PP PTD + Y+V D F ++R S+ R L + PLL++DQ+LQ+S+ +++ + G GE L+ W + LW RD NLYG+HPF + DG+A+G+F NSN E QPAPA+T+ T GGI+DF+IF G P+ +QY +++G PM+PPYWSLGFHL +WGY + + Q A FP+ QW+D+DY + + FT D ++F L V+E + ++V I+DPGIS TS Y P+ EG +FI++ T Q ++GKVWPG T +PDFT+P+ +W + I FY VP+DGLWIDMNEP+NF GS GC ++ +NPF+VPD+ L + T+C S+ + HYNLHNLYG TE++ T+ ALLK G RPF+LSRS+F G G+Y+ HWTGD + W+QLR SI VL F ++G+P+ GADICGF G++ EELC+RW QLGAFYPFMRNHN + Q+P + +AQ M++ + RY++LP YTL ++ + TV RP+ EF D +I+ QF+ GSS++ +PV+ GA + AY P G+WY + G+ +S G +L L APLD IN+ +RGG I+P + ++ N L ++ D A G+L+WDDGES+ ++ R YS++ F A EQ+L
Sbjct: 77 AGKDCGIAVE-RRFDCARDRALTQSQCEQRGCCY---VPLPKSAFSGP---PWCFYPVSYPGYRMGPLVPTSRGQEANLTRSTSSYLPRDVQTLTLEVMAEEAGRLHIALKDPSQDRYEVPFVAVPP--TTPTDTQSFLYDVEFQPD-PFGFVVRRASNGRVLLNTTVAPLLFADQYLQLSTTLASSLVSGFGEHYTGLTLDLN--WTSVTLWNRDMAPHRDTNLYGSHPFCLVQEGDGQAYGIFLLNSNAMEVVSQPAPALTWVTTGGIMDFYIFLGPDPQSVAQQYHQVIGYPMMPPYWSLGFHLCRWGYTSTNATRAVVQHMHQANFPLDVQWNDLDYADKRRVFTFDPQRFGDLPEMVKEFHSE-GMKYVLILDPGISSTSPPGSYKPFDEGERRGVFIRN-LTGQTLIGKVWPGPTAFPDFTNPDAVSWWEECIADFYAKVPVDGLWIDMNEPANFVQGSLLGCP-DTDLENPFYVPDMIGGQLNAGTLCMSS-QQSLSAHYNLHNLYGLTEAMATHSALLKA-RGSRPFVLSRSSFPGLGRYSGHWTGDVHSDWEQLRYSIPAVLLFGLYGVPLVGADICGFEGDTSEELCVRWTQLGAFYPFMRNHNDRSNAPQEPYVFGQQAQAAMRSVMMLRYSLLPFLYTLFHQAQTSASTVARPLFLEFPFDPNCQTIDQQFLWGSSLLISPVLEQGAVELTAYLPPGMWYSLYDGQPYNS--RGQYLLLSAPLDTINVHVRGGRIIPQQMPALTTTASRSNPFLLTVALSSDGWAVGELFWDDGESLGTFERGDYSFIIFTA--EQVL 865
BLAST of Lysosomal alpha-glucosidase vs. Ensembl Cavefish
Match: ganab (glucosidase, alpha; neutral AB [Source:ZFIN;Acc:ZDB-GENE-110930-9]) HSP 1 Score: 268.855 bits (686), Expect = 6.483e-76 Identity = 212/729 (29.08%), Postives = 326/729 (44.72%), Query Frame = 3
Query: 741 DGSFSIEIKRKSDDRSLFKSFGPLLYSDQFLQISSIMSTDQIYGIGEQNRPFLHQLSNGWERIGLWARD-------NPIETFMNLYGAHPFYIGMPDDGKAFGVFHFNSNG----------------------QEFALQPAPAVTYRTIGGILDFFIFTGGSPEETVKQYLELVGKPMLPPYWSLGFHLSKWGYKNIEQLSEIFQRNINAGFPIGTQWSDIDYMENYKDFTVDKEKFNGLGRFVREIKQKYNSRFVAIVDPGISCTSGNEYPPYYEGVFMDIFIKDSQTNQPIVGKVWPGETVYPDFTHPNISMYWYKQIQRFYNDVPIDGL--WIDMNEPSNFYDGSKRGCQGNSKYDNPFFVPDIRDQSLISKTICSSALHYNNEKHYNLHNLYG-YTESVTTNKALLKTIHG-KRPFILSRSTFSGSGKYTFHWTGDNQASWDQLRNSIRQVLNFNMFGIPMTGADICGFNGNSDEELCIRWMQLGAFYPFMRNHNAINQRDQDPASWNTEAQKIMKTAISNRYAMLPLFYTLLVKSHMNGGTVMRPMSFEFFSDKVTHSIELQFMIGSSIMATPVVYPGARSVDAYFP--AGVWYDFHSGKLLHSDAEGAWLTLDAPLDKINIALRGGHIVPLLDSNRNSKSNIPNK-MTLYCGMAQDMSASGDLYWDDGESIDSYSRNRY-----SYV-----SFNASPEQLLITPVYFGYDLLPVLNKIVILG 2789
DG + K +D K GP S F S+ + +YGI E + ++G E L+ D NP M LYGA P + + + G+F N+ Q + P V + + GI+D FI G P + QY L G PP L +H +W Y + + + + + P W DI++ + + FT D KF ++ IK K + VAIVDP I SG Y + E + ++K+ G WPG + YPDFT+P + +W + ++ L W DMNEPS F P++ T+ A+H + E H ++HNLYG Y + T + ++ G +RPF+L+R+ F+GS +Y WTGDN A WD L+ S+ L+ + GI + GAD+ GF + EL +RW Q GA+ PF R H ++ ++P + E +++ AI RYA+LP +Y ++H G VMR + E+ D T +I+ +F+IG ++ V GAR V AY P VWYD H+ + H+ A+ L + + I + RGG I+P R S + N TL+ ++ A G+LY DDG + + ++ + S+V S N PE T + + K++ILG
Sbjct: 186 DGLWEETFKSHTDS----KPNGPTAVSLDF----SLPGVEHVYGIPEHADNLKLKTTDGGEPYRLYNLDVFQYELYNP----MALYGAVPVMLAH-NTQRTMGIFWLNAAETWVDISSNTAGKTVFGKMLDFVQGSSETPQTDVRWISESGIIDVFIMLGPKPSDVFTQYASLTGTQSFPPLTGLAYHQCRWNYNDQDDVKAVDEGFDQHDIPCDFIWLDIEHTDGKRYFTWDPHKFPQPKDMLQGIKDK-RRKMVAIVDPHIKVDSG--YKIHNEIRDKNFYVKNKDGGD-YEGWCWPGNSGYPDFTNPEMRAWWASMFAYDQYEGSMENLYTWNDMNEPSVFNG------------------PEV--------TMHKDAMHGHWE-HRDVHNLYGLYVQHRATAEGQIQRSGGTERPFVLTRAFFAGSQRYGAVWTGDNAAEWDHLKISVPMCLSLGLVGISLCGADVGGFFKSPSAELLVRWYQTGAYQPFFRAHAHLDTPRREPWLFGPENTALIREAIRQRYALLPYWYQQFYQAHKTGQPVMRALWVEYPKDTATFTIDDEFLIGRDLLVHTVTEEGARGVTAYLPGLGEVWYDVHTFQ-KHNGAQN--LYIPVTMSSIPVFQRGGSIIPRKVRVRRSSECMENDPYTLFVALSPQRVAEGELYIDDGHTFNFQTKKEFIHRGLSFVNNVLTSKNLCPESNFSTKSW--------IEKVLILG 859
BLAST of Lysosomal alpha-glucosidase vs. Ensembl Cavefish
Match: ENSAMXT00000016120.2 (neutral alpha-glucosidase AB-like [Source:NCBI gene;Acc:103032339]) HSP 1 Score: 261.536 bits (667), Expect = 2.699e-73 Identity = 187/634 (29.50%), Postives = 291/634 (45.90%), Query Frame = 3
Query: 843 SIMSTDQIYGIGEQNRPFLHQLSNGWERIGLWARDN---PIETFMNLYGAHPFYIGMPDDGKAFGVFHFNS-----------NGQEF---------ALQPAPAVTYRTIG--GILDFFIFTGGSPEETVKQYLELVGKPMLPPYWSLGFHLSKWGYKNIEQLSEIFQRNINAGFPIGTQWSDIDYMENYKDFTVDKEKFNGLGRFVREIKQKYNSRFVAIVDPGISCTSGNEYPPYYEGVFMDIFIKDSQTNQPIVGKVWPGETVYPDFTHPNISMYWYKQIQRFYNDVPIDGL--WIDMNEPSNFYDGSKRGCQGNSKYDNPFFVPDIRDQSLISKTICSSALHYNNEKHYNLHNLYG-YTESVTTNKALLKTIHGKRPFILSRSTFSGSGKYTFHWTGDNQASWDQLRNSIRQVLNFNMFGIPMTGADICGFNGNSDEELCIRWMQLGAFYPFMRNHNAINQRDQDPASWNTEAQKIMKTAISNRYAMLPLFYTLLVKSHMNGGTVMRPMSFEFFSDKVTHSIELQFMIGSSIMATPVVYPGARSVDAYFPAG--VWYDFHSGKLLHSDAEGAWLTLDAPLDKINIALRGGHIVPLLDSNRNSKSNIPNK-MTLYCGMAQDMSASGDLYWDD 2651
S + +YGI E + ++G E L+ D + M LYGA P I + + GVF N+ +G+ AP R I GI+D F +G P + QY L G P +LG+H +W Y + E + + + P W DI++ + + FT D KF+ ++ + K + VAIVDP I SG Y + E ++K+ G WPG + YPDFT+P + +W + ++ L W DMNEPS F P++ T+ A+H N E H ++HN+YG Y + T + ++ +RPF+L+R+ F+GS +Y WTGDN A W L+ SI L+ + GI GAD+ GF + EL +RW Q GA+ PF R H ++ ++P + + +++ AI RY +LP +Y L ++ G VMRP+ E+ D T +IE ++++G ++ PV GA+ V A+ P VWYD H+ + H A+ L + + I + RGG I+ D R S + + N TLY ++ +A G++Y DD
Sbjct: 221 SFPGVEHLYGIPEHADTLKLKNTDGGEPYRLYNLDVFQYELNNLMALYGAVPVIISHNTE-RTMGVFWLNAAESWVDISSSTSGKTVFGKMMDFVQGSNEAPQTDVRWISESGIIDVFFMSGPRPNDVFAQYASLTGTQAFPALATLGYHQCRWNYNDQEDVKAVDEGFDEHDIPYDFIWLDIEHTDGKRYFTWDPNKFSQPKEMLQGLMDK-RRKMVAIVDPHIKVDSG--YSIHNEIRSKGFYVKNKDGGD-YEGWCWPGNSGYPDFTNPEMRAWWASKFAYDQYTGSMENLFTWNDMNEPSVFNG------------------PEV--------TMHKDAMHGNWE-HRDVHNIYGIYVQRATAEGQIQRSGGTERPFVLTRAFFAGSQRYGAVWTGDNAAEWGHLKISIPMCLSLGLVGISFCGADVGGFFKHPSTELLVRWYQAGAYQPFFRAHAHLDTPRREPWLFGPDNTALIREAIRQRYTLLPYWYQLFYNAYRTGQPVMRPLWVEYTEDPDTFAIEDEYLLGKDLLVHPVTEEGAKGVTAFLPGKGEVWYDVHTFQ-KHKGAQN--LYIPVTMSSIPVFQRGGSIISRKDRVRRSSACMENDPYTLYVALSPQGTAEGEIYIDD 819
BLAST of Lysosomal alpha-glucosidase vs. Ensembl Sea Lamprey
Match: ENSPMAT00000005523.1 (pep scaffold:Pmarinus_7.0:GL480123:76219:97698:-1 gene:ENSPMAG00000005011.1 transcript:ENSPMAT00000005523.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 450.669 bits (1158), Expect = 3.422e-146 Identity = 254/709 (35.83%), Postives = 369/709 (52.05%), Query Frame = 3
Query: 741 DGSFSIEIKRKSDDRSLFKSFGP-LLYSDQFLQISSIMSTDQIYGIGEQNRPFLHQLSNGWERIGLWARDNPIETFMNLYGAHPFYIGMPDDGKAFGVFHFNSNGQEFALQPAPAVTYRTIGGILDFFIFTGGSPEETVKQYLELVGKPMLPPYWSLGFHLSKWGYKNIEQLSEIFQRNINAGFPIGTQWSDIDYMENYKDFTVDKEKFNGLGRFVREIKQKYNSRFVAIVDPGISCTSGNEYPPYYEGVFMDIFIKDSQTNQPIVGKVWPGETVYPDFTHPNISMYWYKQIQRFY-------NDVPIDGLWIDM-------------------NEPS------NFYDGSKRGCQGNSKYD------------------------NPFFVPDI-------RDQSLISKTICSSALHY----NNEKHYNLHNLYGYTESVTTNKALLKTIHGKRPFILSRSTFSGSGKYTFHWTGDNQASWDQLRNSIRQVLNFNMFGIPMTGADICGFNGNSDEELCIRWMQLGAFYPFMRNHNAINQRDQDPASWNTEAQKIMKTAISNRYAMLPLFYTLLVKSHMNGGTVMRPMSFEFFSDKVTHSIELQFMIGSSIMATPVVYPGARSVDAYFPAGVWYDFHSGKLLHSDAEGAWLTLDAPLDKINIALRGGHIVPLLDSNRNSKSNIPNKMTLYCGMAQDMSASGDLYWDDGESI 2663
D F I + RKS + ++ S P ++D+F+Q+S ++ ++ IYG GE Q W L + N YG HP+Y+ + D A V FNSN + LQP PAVTYRT GGILDF+ G SPEE +QY L+G+P +PPYWSLGF L ++GY N ++ ++ AG P TQ+ DIDYM DFT++KEKF +R + + R + I+DP I+ YP Y GV D+++K Q + GK + PDF + +W ++I+ FY + + DGLWIDM NEP Y S C + D N ++ I D++ KT+C ALH + +HYN+H+LYG++++ T AL + GKR +++RSTF GSG+++ HW GDN A WD L SI ++ F++FGI TGADICGF G+S+ +LC RWMQLGAFYP+ RNHN QDP +W+T ++ K + RY++LP YTL+ ++H +G TV+RP+ EF SD T +I QF+ G +++ TPV+ +V Y P WYD+ + K + ++AP D IN+ +RGG+I+P +K + M+L +A+G L+WDDG+ I
Sbjct: 4 DSPFGIRVIRKSTGKVIWNSQAPGFTFTDRFIQMSVLLPSENIYGFGETEHDTFKQ---PWYVHILGVCAVYPQYRANAYGVHPYYMSVEDGANAHSVLLFNSNAMDIVLQPNPAVTYRTTGGILDFYFTLGPSPEEVTQQYTALIGRPAMPPYWSLGFQLCRYGYANDTEILNLYNDMKAAGVPYDTQYGDIDYMVRQLDFTLNKEKFGNFPNLIRHMHDE-GMRIIIILDPAIAANETEPYPAYTRGVENDVYMKRPD-GQIMFGKHYRAYAAMPDFFRERTARWWQREIEEFYRNPTTPTDSMEFDGLWIDMNEPASFVHGSTSGCEDKELNEPPYMPRAFELYFSSLANCHTSIPLDSQHRYTTTRSSQQVTTHSHMCGDNNQTYIQKILHRKSVQADETSHHKTMCMEALHELPDGSKVQHYNVHSLYGWSQTKPTLDAL-HNVTGKRGIVVTRSTFPGSGQWSGHWLGDNSARWDNLDKSIIGMMEFSLFGITYTGADICGFFGDSNYDLCARWMQLGAFYPYSRNHNGKGNIRQDPVAWDTAFAEMSKRVLEIRYSLLPYLYTLMHEAHAHGSTVVRPLLHEFLSDNETWAISKQFLWGPALLITPVLEEEKVTVRGYVPDARWYDYQTMKGAQLTERKEYKEMNAPWDTINLHVRGGYILPTQQPANTTKYSRQKPMSLLVAPDSSGTATGSLFWDDGDGI 706
BLAST of Lysosomal alpha-glucosidase vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008911.1 (pep scaffold:Pmarinus_7.0:GL496136:1712:3787:1 gene:ENSPMAG00000008065.1 transcript:ENSPMAT00000008911.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 247.669 bits (631), Expect = 2.465e-73 Identity = 157/465 (33.76%), Postives = 238/465 (51.18%), Query Frame = 3
Query: 813 LYSDQFLQISSIMSTD-QIYGIGEQNRPFLHQLSNGWERIGLWARDNPI--ETFM----NLYGAHPFYIGMPDDGKAFGVFHFNSNGQEFAL---QPAPAVTYRTI--GGILDFFIFTGGSPEETVKQYLELVGKPMLPPYWSLGFHLSKWGYKNIEQLSEIFQRNINAGFPIGTQWSDIDYMENYKDFTVDKEKFNGLGRFVREIKQKYNSRFVAIVDPGISCTSGNEYPPYYEGVFMDIFIKDSQTNQPIVGKVWPGETVYPDFTHPNISMYWYKQIQRFYNDVPIDGLWIDMNEPSNFYDGSKRGCQGNSKYDNPF-----FVPDIRDQSLISKTICSSALHYNNEKHYNLHNLYGYTESVTTNKALLKTIHGK--RPFILSRSTFSGSGKYTFHWTGDNQASWDQLRNSIRQVLNFNMFGIPMTGADICGFNGNSDEELCIRWMQLGAFYPFMRNHNAINQ 2150
L+ D L+IS I S + Y I Q+ L + +G +W+RD P ET N+YG HPF++ G GV + ++ Q++ + Q ++ TI GG+ D ++ SP++ V Y L+G+P++ P W+LG++ +WGY +++L + P+ QWSDID+M Y+ F D+ F L FV ++ K ++V IVD GI+ ++Y + E +F I +VWP + +PD+T+P+ ++ + V DGLW DMNE SNF G+ C N + D P + P RD + K++ LH N + H+ YG E +K H K R FI+ RS+F+G GK+ W GDN + + SI V+ N+FGIP+ GADICGF G+++ ELC RW +GA+YPF RNHN Q
Sbjct: 1 LFLDTGLKISEITSKSFEYYQIIHQST-LLQLVEDG--VYSMWSRDQPTPEETGTLPGSNMYGTHPFFMYKHKVGAWTGVLYKLAHAQDWWVKNNQAKGSIDISTIATGGVADIYVIQAQSPDDIVNNYFRLIGRPVMVPQWALGWNQCRWGYDTLDKLKGYDDNKL----PLDVQWSDIDWMNKYRSFEFDQVNFKDLPSFVDDL-HKSGRKYVPIVDAGIAYRPDSDYKAFQENLFTTI------------NEVWPNDASFPDYTNPDTG-----HLEDVHTQVAFDGLWEDMNEASNFCQGA---CYRNQQVDKPVKQNLPYTPTGRDLEI--KSMPLDTLHSNGVLQLDAHSYYGTQE--------VKATHSKNMRTFIIERSSFAGMGKFASRWLGDNFSEDKYMGYSISGVMMMNIFGIPLAGADICGFIGDTNPELCARWHHVGAYYPFSRNHNNWGQ 427
BLAST of Lysosomal alpha-glucosidase vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008912.1 (pep scaffold:Pmarinus_7.0:GL496136:2540:3769:1 gene:ENSPMAG00000008065.1 transcript:ENSPMAT00000008912.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 237.269 bits (604), Expect = 3.776e-70 Identity = 142/420 (33.81%), Postives = 215/420 (51.19%), Query Frame = 3
Query: 933 LWARDNPI--ETFM----NLYGAHPFYIGMPDDGKAFGVFHFNSNGQEFAL---QPAPAVTYRTI--GGILDFFIFTGGSPEETVKQYLELVGKPMLPPYWSLGFHLSKWGYKNIEQLSEIFQRNINAGFPIGTQWSDIDYMENYKDFTVDKEKFNGLGRFVREIKQKYNSRFVAIVDPGISCTSGNEYPPYYEGVFMDIFIKDSQTNQPIVGKVWPGETVYPDFTHPNISMYWYKQIQRFYNDVPIDGLWIDMNEPSNFYDGSKRGCQGNSKYDNPF-----FVPDIRDQSLISKTICSSALHYNNEKHYNLHNLYGYTESVTTNKALLKTIHGK--RPFILSRSTFSGSGKYTFHWTGDNQASWDQLRNSIRQVLNFNMFGIPMTGADICGFNGNSDEELCIRWMQLGAFYPFMRNHN 2138
+W+RD P ET N+YG HPF++ G GV + ++ Q++ + Q ++ TI GG+ D ++ SP++ V Y L+G+P++ P W+LG++ +WGY +++L + P+ QWSDID+M Y+ F D+ F L FV ++ K ++V IVD GI+ ++Y + E VWP + +PD+T+P+ ++ + V DGLW DMNE SNF G+ C N + D P + P RD + K++ LH N + H+ YG E +K H K R FI+ RS+F+G GK+ W GDN + + SI V+ N+FGIP+ GADICGF G+++ ELC RW +GA+YPF RNHN
Sbjct: 2 MWSRDQPTPEETGTLPGSNMYGTHPFFMYKHKVGAWTGVLYKLAHAQDWWVKNNQAKGSIDISTIATGGVADIYVIQAQSPDDIVNNYFRLIGRPVMVPQWALGWNQCRWGYDTLDKLKGYDDNKL----PLDVQWSDIDWMNKYRSFEFDQVNFKDLPSFVDDL-HKSGRKYVPIVDAGIAYRPDSDYKAFQEA------------------HVWPNDASFPDYTNPDTG-----HLEDVHTQVAFDGLWEDMNEASNFCQGA---CYRNQQVDKPVKQNLPYTPTGRDLEI--KSMPLDTLHSNGVLQLDAHSYYGTQE--------VKATHSKNMRTFIIERSSFAGMGKFASRWLGDNFSEDKYMGYSISGVMMMNIFGIPLAGADICGFIGDTNPELCARWHHVGAYYPFSRNHN 380
BLAST of Lysosomal alpha-glucosidase vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008215.1 (pep scaffold:Pmarinus_7.0:GL476974:10756:37747:1 gene:ENSPMAG00000007408.1 transcript:ENSPMAT00000008215.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 249.98 bits (637), Expect = 5.596e-70 Identity = 174/589 (29.54%), Postives = 284/589 (48.22%), Query Frame = 3
Query: 1080 AVTYRTIGGILDFFIFTGGSPEETVKQYLEL----VGKPMLPPYWSLGFHLSKWGYKNIEQLSEIFQRNINAGF-----PIGTQWSDIDYMENYKDFTVDKEKFNGLGRFVREIKQKYNSRFVAIVDPGISCTSGNEYPPYYEGVFMDIFIKDSQTNQPIVGKVWP-GETVYPDFTHPNISMYWYKQIQ--RFYNDVPIDGLWIDMNEPSNFYDGSKRGCQGNSKYDNPFFVPDIRDQSLISKTICSSALHYNNEKHYNLHNLYGYTESVTTNKALLKTIHG-KRPFILSRSTFSGSGKYTFHWTGDNQASWDQLRNSIRQVLNFNMFGIPMTGADICGF-NGNSDEELCIRWMQLGAFYPFMRNHNAINQRDQDPASWNTEAQKIMKTAISNRYAMLPLFYTLLVKSHMNGGTVMRPMSFEFFSDKVTHSIELQFMIGSSIMATPVVYPGARSVDAYFPA--GVWYDFHSGKLLHSDAEGAWLTLDAPLDKINIALRGGHIVPLLDSNRNSKSNIP-NKMTLYCGMAQDMSASGDLYWDDGESIDSYSRNRYSYVSFNASPEQLLITPV--YFGYDLLPVLNKIVILG 2789
V + + G +D F+ G +P + +QY L G LPP ++LG+H S+W + +E R ++ GF W DI++ + + FT D ++F ++ K + V IVDP I NEY Y EG FI++ + G+ WP G + Y D T+P++ ++ + + N W DMNEP+ F+ I KT+ + LH+ +H + HNLYG+ + + + L++ G +RPF+L+RS F+GS +Y WTGD+ A+WD LR S +L+ ++ GI + GAD+ GF + + +RW Q G+ PF R H+A +R+ P +K+++ A++ RYA+LP +YT ++H G VMRP+ EF SD+ T +E Q+MIG++++A PV +V Y P WYD HS K + + A + I + R G I+P + R+S + + TL + A G+L+ DDG S ++ R+ + F+ S L + + + K+VI+G
Sbjct: 313 GVRWMSESGTIDVFVLPGPTPCDVFRQYAGLTVGSAGTQALPPLFALGYHHSRWA-----EQTEADTREVDGGFRRSRIACDVLWLDIEHTDGKRYFTWDADRFPDPVGLQNDLAAK-GRKVVTIVDPHIKVD--NEYFVYSEGKARGYFIRNPDGSN-YEGRCWPEGLSSYLDLTNPDVRDWYASKFDYCNYKNSTAALFTWNDMNEPAVFHS--------------------------IEKTVPRNCLHWGGWEHRDNHNLYGFYQQMASAAGLVRRSGGLERPFVLTRSFFAGSQRYGAVWTGDSLATWDYLRMSTPMLLSLSVCGISLCGADVGGFFEEPQSQAMVLRWFQAGSLQPFFRVHSAKVRRE--PWLMGASGEKLIRRAVALRYALLPYWYTQFYRAHRTGQPVMRPLWVEFPSDEETFVLEDQYMIGNALLARPVTEDNVNTVQVYLPGRNEAWYDIHSRKRYPAPGKFA---QRVNMSSIPLYQRAGTIIPQKLNLRDSTELMKEDPYTLLVAVDPGGCAEGELFIDDGHSFLYKTQQRFLHRGFSYSDSTLTCKSLDSAGSMECRVQVEKVVIVG 861
BLAST of Lysosomal alpha-glucosidase vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008908.1 (pep scaffold:Pmarinus_7.0:GL496136:110:3787:1 gene:ENSPMAG00000008065.1 transcript:ENSPMAT00000008908.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 190.274 bits (482), Expect = 8.345e-54 Identity = 123/359 (34.26%), Postives = 177/359 (49.30%), Query Frame = 3
Query: 1227 WGYKNIEQLSEIFQRNINAGFPIGTQWSDIDYMENYKDFTVDKEKFNGLGRFVREIKQKYNSRFVAIVDPGISCTSGNEYPPYYEGVFMDIFIKDSQT-----NQPIVGKVWPGETVYPDFTHPNISMYWYKQIQRFYNDVPIDGLWIDMNEPSNFYDGS---------------KRGCQGNSKYDNPFFVPDIRDQSLISKTIC----------------------------SSALHYNNEKHYNLHNLYGYTESVTTNKALLKTIHGK---RPFILSRSTFSGSGKYTFHWTGDNQASWDQLRNSIRQVLNFNMFGIPMTGADICGFNGNSDEELCIRWMQLGAFYPFMRNHNAINQ 2150
W Y N + + E+ AG P+ T + DI YM++YKDF+VD + F GL + + + QK V I+D IS N+ +DIFIK S N I+ KVWP + V+ D+ +P +W Y+ V DG+WIDMNEP+ F G KR + + + ++ + +DQS+ S +S + E YN+H+LYG + T + + K + RP+IL+RSTF+ SG+Y HW GDN W+ +R SI V+ N+FGIP+ GADICGF G+++ ELC RW +GA+YPF RNHN Q
Sbjct: 1 WKYINQDMVEEVIDNYFAAGMPLETMYLDIPYMKSYKDFSVDTKAF-GLAKRLHDANQK----LVVILDAAISADDVNDDVYQKGSTELDIFIKSSMYKSKTYNNNIISKVWPDKAVFIDWFNPKSLDFW------TYDLVDYDGIWIDMNEPTTFGHGEIKPDDAEPVTPTLAKKRLMSDDGETVDWYY--EFKDQSVGSTFTLPFIPGYVQSDDGKPGPYNGNFDYMTLSLNSTIPSLGETSYNVHSLYGLMMAKRTFEHVTKMEAKRPDERPYILTRSTFASSGRYASHWLGDNWRKWEYMRYSIAGVMMMNIFGIPLAGADICGFIGDTNPELCARWHHVGAYYPFSRNHNNWGQ 346
BLAST of Lysosomal alpha-glucosidase vs. Ensembl Yeast
Match: ROT2 (Glucosidase II catalytic subunit; required to trim the final glucose in N-linked glycans; required for normal cell wall synthesis; mutations in rot2 suppress tor2 mutations, and are synthetically lethal with rot1 mutations [Source:SGD;Acc:S000000433]) HSP 1 Score: 232.261 bits (591), Expect = 6.306e-64 Identity = 184/639 (28.79%), Postives = 290/639 (45.38%), Query Frame = 3
Query: 843 SIMSTDQIYGIGEQNRPF-LHQLSNGWERIGLWARDN---PIETFMNLYGAHPFYIGMPDDGKAFGVFHFNSNGQEFALQ-----PAPAVTYRTIGGILDFFIFTGGSPEETVKQYLELVGKPMLPPYWSLGFHLSKWGYKNIEQLSEIFQRNINAGFPIGTQWSDIDYMENYKDFTVDKEKFNGLGRFVREIKQKYNSRFVAIVDPGISCTSGNEYPPYYEGVFMDIFIKDSQTNQPIVGKVWPGETVYPDFTHPNISMYWYKQIQRFYNDVPID----GLWIDMNEPSNFYDGSKRGCQGNSKYDNPFFVPDIRDQSLISKTICSSALHYNNEKHYNLHNLYGYTESVTTNKALLKTIHG---KRPFILSRSTFSGSGKYTFHWTGDNQASWDQLRNSIRQVLNFNMFGIPMTGADICGFNGNSDEELCIRWMQLGAFYPFMRNHNAINQRDQDPASWNTEAQKIMKTAISNRYAMLPLFYTLLVKSHMNGGTVMRPMSFEFFSDKVTHSIELQFMIGSS-IMATPVVYPGARSVDAYFPAGVWYDFHS-GKLLHSDAEGAWLTLDAPLDKINIALRGGHIVPLLDSNRNSKSNIPNK-MTLYCGMAQDMSASGDLYWDDGESIDSYSRNRYSYVSF 2702
S M + +YGI E L S G E L+ D I T +YG+ PF + +F N+ ++ + + G++D + G + ++ +L G+P LPP S+G+H +W Y + + + + P W D++Y + K FT + F + + +K V ++DP + +Y + ++ +KD N VG WPG +++ D W +RF D+P D +W DMNEPS +DG + + +DN Y E+ ++HN+YG + T A+ K+I+ KRPF+L+R+ F+GS + WTGDN A+WD L+ SI VL+ N+ G+P GADI GF + EL RW Q G +YPF R H I+ + ++P +N + I++ I RY +LP YT+ KS + G +M PM E + I+ QF +S ++ PV PG + FP G++Y+F S +++ + + APLDKI + + GGHI+ + D R S + N + + A GDLY DDGE+ Y R Y F
Sbjct: 264 SFMGSTNVYGIPEHATSLRLMDTSGGKEPYRLFNVDVFEYNIGTSQPMYGSIPFMFS----SSSTSIFWVNAADTWVDIKYDTSKNKTMTHWISENGVIDVVMSLGPDIPTIIDKFTDLTGRPFLPPISSIGYHQCRWNYNDEMDVLTVDSQMDAHMIPYDFIWLDLEYTNDKKYFTWKQHSFPN-PKRLLSKLKKLGRNLVVLIDPHLK----KDYEISDRVINENVAVKDHNGND-YVGHCWPGNSIWIDTISKYGQKIWKSFFERFM-DLPADLTNLFIWNDMNEPS-IFDGPETTAPKDLIHDN-----------------------YIEER--SVHNIYGLSVHEATYDAI-KSIYSPSDKRPFLLTRAFFAGSQRTAATWTGDNVANWDYLKISIPMVLSNNIAGMPFIGADIAGFAEDPTPELIARWYQAGLWYPFFRAHAHIDTKRREPYLFNEPLKSIVRDIIQLRYFLLPTLYTMFHKSSVTGFPIMNPMFIEHPEFAELYHIDNQFYWSNSGLLVKPVTEPGQSETEMVFPPGIFYEFASLHSFINNGTDLIEKNISAPLDKIPLFIEGGHIITMKDKYRRSSMLMKNDPYVIVIAPDTEGRAVGDLYVDDGETF-GYQRGEYVETQF 863
BLAST of Lysosomal alpha-glucosidase vs. Ensembl Nematostella
Match: EDO41811 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S392]) HSP 1 Score: 646.736 bits (1667), Expect = 0.000e+0 Identity = 349/800 (43.62%), Postives = 478/800 (59.75%), Query Frame = 3
Query: 348 RLDCYPESGANQDSCIARGCCW----SEPMRL--SNKLQGV--PLSIPYCYYPTDYSNYMIKSLRKSSRGIKAKLVRESPSYLPYEVAMLDLEVIYETKSRTRVRITDPKQKRYEVPMKLSPPDLKPPTDREYEVIIPSDGSFSIEIKRKSDDRSLFKS-FGPLLYSDQFLQISSIMSTDQIYGIGEQNRPFLHQLSNGWERIGLWARD--NPIETFMNLYGAHPFYIGMPDDGKAFGVFHFNSNGQEFALQPAPAVTYRTIGGILDFFIFTGGSPEETVKQYLELVGKPMLPPYWSLGFHLSKWGYKNIEQLSEIFQRNINAGFPIGTQWSDIDYMENYKDFTVDKEKFNGLGRFVREIKQKYNSRFVAIVDPGISCTS-GNEYPPYYEGVFMDIFIKDSQTNQPIVGKVWPGETVYPDFTHPNISMYWYKQIQRFYNDVPIDGLWIDMNEPSNFYDGSKRGCQGNSKYDNPFFVPDIRDQSLISKTICSSALHYNNEKHYNLHNLYGYTESVTTNKALLKTIHGKRPFILSRSTFSGSGKYTFHWTGDNQASWDQLRNSIRQVLNFNMFGIPMTGADICGFNGNSDEELCIRWMQLGAFYPFMRNHNAINQRDQDPASWNTEAQKIMKTAISNRYAMLPLFYTLLVKSHMNGGTVMRPMSFEFFSDKVTHSIELQFMIGSSIMATPVVYPGARSVDAYFPAGVWYDFH-----------SGKLLHSDAEGAWLTLDAP-LDKINIALRGGHIVPLLDSNRNSKSNIPNKMTLYCGMA--QDMSASGDLYWDDGESIDS 2669
R DC+PE+GA ++ C ARGCCW + P R ++K GV PL IPYC+YP +Y Y + S + G K L P+ LP + + ++ + I DP KRYEVP+ +P + ++Y+V S F I + RKS LF S G +++ DQFLQISS++ + IYG+GE F +L+ W R ++ARD P NLYG HPFY+ + +DG A G+F NSN E LQP PA+TYR++GG+LDF++F G +PE +QY+ L+GKP LPPYW LG+HL +WGY N+ + + P QW+DI+YM+++ DFTVD + GLG FV+++ +Y+ ++ IVDPGIS T YPPY +G+ M +F+ S PIVG+VWPG TVYPDF +P+ YW KQI +F++ VP DGLWIDMNEPSNF GS GC N+K+DNP + P I LI KT+C SA HY +HY++H+LYGYTE+V T A L++I GKR ++SRSTF SG++ HW GDNQA+W+ + S+ +LN NMFGIP+ GADICGF GN++ ELC RW QLGAFYPF RNHN QDPAS+ + + + + RY MLP YTL ++ G TV RP+ FEF D T +I+ QFM GSS++ TPV+ GA V YFP WY+ + +L + G + L P L + +RGG I+ + + ++ N L A+G L+ DDGES+ +
Sbjct: 6 RFDCFPEAGATEELCQARGCCWVPAENRPPRPEPTSKPTGVNAPLDIPYCFYPLNYG-YSLTSKVATKTGYKKTLC---PTKLPIKHQTSERKIKMTNLFKLHGDIYDPANKRYEVPIP-TPMITQKSNSQDYDVSFTSF-PFGISVTRKSTGTVLFNSTVGGMIFEDQFLQISSLLPSSNIYGLGEHADAF--KLNVTWRRDTMFARDVATPEGMQYNLYGVHPFYLNVENDGNANGLFLLNSNALEVILQPTPAITYRSLGGVLDFYMFLGPTPEAVAQQYITLIGKPRLPPYWGLGYHLCRWGYGNVSRTITVNDNMRRYKIPQDVQWNDIEYMKDHLDFTVDSTNWGGLGDFVKKLHTQYDQHYIPIVDPGISNTQPSGSYPPYSDGLAMGVFVNASN-GGPIVGQVWPGNTVYPDFFNPSTQSYWTKQISQFHDVVPFDGLWIDMNEPSNFVQGSTSGCP-NTKWDNPPYTPHIIGDKLIDKTLCMSARHY-GYRHYDVHSLYGYTETVATMSA-LESIRGKRSMVISRSTFPNSGQHGGHWLGDNQATWESMYLSVPGILNMNMFGIPLVGADICGFLGNTNYELCARWTQLGAFYPFSRNHNTKGATPQDPASFGDKFASMARGVLLTRYRMLPYLYTLFFDAYNMGSTVARPLFFEFPKDAKTLAIDRQFMWGSSLLVTPVLQQGASDVTGYFPDATWYNVYDVRLRAPCAPPGSELQREGSGGQYHKLGCPVLCDTPLHIRGGSIIATQKPDITTAASRKNPFELIVAKTGKDGEPANGRLFLDDGESLGT 793
BLAST of Lysosomal alpha-glucosidase vs. Ensembl Nematostella
Match: EDO48632 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RJ81]) HSP 1 Score: 249.595 bits (636), Expect = 1.139e-69 Identity = 157/505 (31.09%), Postives = 247/505 (48.91%), Query Frame = 3
Query: 1206 LGFHLSKWGYKNIEQLSEIFQRNINAGFPIGTQWSDIDYMENYKDFTVDKEKFNGLGRFVREIKQKYNSRFVAIVDPGISCTSGNEYPPYYEGVFMDIFIKDSQTNQPIVGKVWPGETVYPDFTHPNISMYWYKQIQ--RFYNDVPIDGLWIDMNEPSNFYDGSKRGCQGNSKYDNPFFVPDIRDQSLISKTICSSALHYNNEKHYNLHNLYGYTESVTTNKALLKTIHGK-RPFILSRSTFSGSGKYTFHWTGDNQASWDQLRNSIRQVLNFNMFGIPMTGADICGFNGNSDEELCIRWMQLGAFYPFMRNHNAINQRDQDPASWNTEAQKIMKTAISNRYAMLPLFYTLLVKSHMNGGTVMRPMSFEFFSDKVTHSIELQFMIGSSIMATPVVYPGARSVDAYFPAG--VWYDFHSGKLLHSDAEGAWLTLDAPLDKINIALRGGHIVPLLDSNRNSKS-NIPNKMTLYCGMAQDMSASGDLYWDDGESIDSYSRNRYSYVSF 2702
+ +H +W Y + E + + P W DI++ + + T DK KF I K + V IVDP + TS +Y + E + +IK+ + W G + +PDFT+P I +W +I ++ +W DMNEPS F+ P+I T+ +HY + +H ++HN+YG TN L++ GK RPF+LSR+ F+G+ +Y WTGDN A W L+ SI +L+ + G+P GAD+ GF N + EL RW Q G F PF+R H ++ + ++P ++ + +++ A+ RYA+LPL+YTL + +G ++RP+ E+ DK T +E +F++G ++ PV PG + D Y P WY K+ G + + +PLDKI + RGG I+P + R S S + + TL + A GDLY DD S D Y ++Y+ F
Sbjct: 335 IAYHQCRWNYNDEEDVKSVDDSFDKHDIPYDVLWLDIEHTDGKRYMTWDKVKFPNPEAMQENIASK-GRKMVTIVDPHMKRTS--DYHVHEEATSLGHYIKNKDGGE-YENWCWSGSSSWPDFTNPKIREWWASKISHDQYKGSTTNLFIWNDMNEPSVFHG------------------PEI--------TMHKDTIHYGDWEHRDVHNIYGMYFHKATNLGLIQRSGGKDRPFVLSRAFFAGTQRYGPIWTGDNMAEWSHLKASIPMILSLGVTGLPFAGADVGGFFKNPEPELLARWYQTGVFTPFLRAHAHLDTKRREPWLFDDVYKNVIRDALRTRYALLPLWYTLFFHASQDGTPIIRPLWVEYPEDKSTFKMEDEFLVGDQLLVKPVTEPGQVTSDVYLPGKEQYWYHLDDHKIYRG---GNNVKVASPLDKIPLFQRGGSIIPRKNRIRRSSSLSHDDPYTLTLALDPKGEAEGDLYIDDFHSFD-YKTGAFAYMKF 805
BLAST of Lysosomal alpha-glucosidase vs. Ensembl Nematostella
Match: EDO45756 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RS68]) HSP 1 Score: 62.003 bits (149), Expect = 7.964e-10 Identity = 58/251 (23.11%), Postives = 96/251 (38.25%), Query Frame = 3
Query: 1815 YNLHNL-YGYTESVTTNKALLKTIHGKRPFILSRSTFSGSGKYTFHWTGDNQASWDQ---LRNSIRQVLNFNMFGIPMTGADICGFNG-----------NSDEELCIRWMQLGAFYPFMRNHNAINQRDQDPASWNTEAQKIMKTAISNRYAMLPLFYTLLVKSHMNGGTVMRPMSFEFFSDKVTHSIELQFMIGSSIMATPVVYPGARSV---DAYFPAGVWY-DFHSGKLLHSDAEGAWLTLDAPLDKI 2510
Y L NL YG + N A + + G R I R F D + WD+ LR+ I L + G P D+ G NG D EL +RWMQ F P M Q P + + I K + R + + + + ++RP+ + +D + +++ +F++G + PV+ P + + Y P G W +F K++ G W+ L+ +
Sbjct: 296 YQLKNLTYGNPSFYSANYAKMASKFGDR--IEVRVGHRTQSIPVFVRFLDRTSKWDEKDGLRSVIPATLTLGILGYPFVLPDMIGGNGAQLNSNSDSFNKPDNELYVRWMQAAVFLPTM-------QFSFPPWLYGSNTINIAKRLLDLRAKISETLILFARQVNQSTAPIIRPLWWVAPNDDIALTLDSEFLVGDRFLVAPVLRPYSENKGKHKVYLPQGKWKEEFGDFKVITVPKPGQWIEYTVTLEDL 537
BLAST of Lysosomal alpha-glucosidase vs. Ensembl Medaka
Match: si:ch73-12o23.1 (lysosomal alpha-glucosidase [Source:NCBI gene;Acc:101169174]) HSP 1 Score: 712.605 bits (1838), Expect = 0.000e+0 Identity = 365/848 (43.04%), Postives = 508/848 (59.91%), Query Frame = 3
Query: 324 CQIANNNERLDCYPESGA--NQDSCIARGCCWSEPMRLSNKLQGVPLSIPYCYYPTDYSNYMIKSLRKSSRGIKAKLVRESPSYLPYEVAMLDLEVIYETKSRTRVRITDPKQKRYEVPMKLSPPDLKPPTDREYEVIIPSDGSFSIEIKRKSDDRSLFKS-FGPLLYSDQFLQISSIMSTDQIYGIGEQNRPFLHQLSNGWERIGLWARDNPIETFMNLYGAHPFYIGMPDDGKAFGVFHFNSNGQEFALQPAPAVTYRTIGGILDFFIFTGGSPEETVKQYLELVGKPMLPPYWSLGFHLSKWGYKNIEQLSEIFQRNINAGFPIGTQWSDIDYMENYKDFTVDKEKFNGLGRFVREIKQKYNSRFVAIVDPGISCTS-GNEYPPYYEGVFMDIFIKDSQTNQPIVGKVWPGETVYPDFTHPNISMYWYKQIQRFYNDVPIDGLWIDMNEPSNFYDGSKRGCQGNSKYDNPFFVPDIRDQSLISKTICSSALHYNNEKHYNLHNLYGYTESVTTNKALLKTIHGKRPFILSRSTFSGSGKYTFHWTGDNQASWDQLRNSIRQVLNFNMFGIPMTGADICGFNGNSDEELCIRWMQLGAFYPFMRNHNAINQRDQDPASWNTEAQKIMKTAISNRYAMLPLFYTLLVKSHMNGGTVMRPMSFEFFSDKVTHSIELQFMIGSSIMATPVVYPGARSVDAYFPAGVWYDFHSGKLLHSDAEGAWLTLDAPLDKINIALRGGHIVPLLDSNRNSKSNIPNKMTLYCGMAQDMSASGDLYWDDGESIDSYSRNRYSYVSFNASPEQLLITPVYFGYDLLP--VLNKIVILGFENKSITKFLLNDNDIPFHY 2849
C + R DCYPE G +D C AR CC+ SN Q +P+C+YP D+ +Y + S + G K LV+E +Y P ++ +++E ET +R VR+TDP R+EVP+ + P K + ++ S F I +KR+S L + PL Y+DQFLQ S+++ T IYG+GE FLH ++ W + +WARD P NLYG HPFY+ M +DG A G F NSN + LQP PA+T+RTIGGILDF++F G P V QYLE++G P +P YW+LG+HL +WGY + E+ + N G P TQW+DIDYM+ DFT D +KF L VR++ +N +V ++DPGIS T Y P+ EG+ +FIKD++ + ++GKVWPG T YPDF+ +W+ +Q+F+N VP DGLWIDMNEPSNF DGS GC NS +NP + P + L +KT+C++A HYNLH+LYG E+ + AL K + KRPF++SRSTF G Y+ HW GDN++ W L SI +L FN+ GIP+ GADICGF+ EELC+RW QLGAFYPF RNHN+I+ + QDP +++ A+ MK A+ RY++ P YTL +H +G +V RP+ FEF D T+ I+ QF+ G S++ TPV+ PG V YFPAG+WYDF++G LHS E + L APLDKIN+ LR G ++P N + + L ++ + SA GDL+WDDGESID++ N+YSY+ F+ + + + + V + + G K +K L+N D PF Y
Sbjct: 113 CSAISEPWRFDCYPERGVVVTKDMCEARNCCFIPASSSSNAGQSRKNGVPWCFYPPDFPSYSLVSTDDTLMGQKGTLVKEVKTYYPGDILTVEVETRQETDTRLHVRMTDPSNPRFEVPISVPNPTKK--AENPAYIVELSKQPFGITVKRRSTGVLLLNTTVAPLFYADQFLQFSTVLPTQFIYGLGEHRSTFLHDMN--WNTLTMWARDVPPMEKTNLYGVHPFYLAMEEDGNAHGFFLLNSNAMDVVLQPTPALTWRTIGGILDFYVFLGPDPGSVVSQYLEVIGNPAMPIYWALGYHLCRWGYNSSNSTWEVVKSLRNYGIPQDTQWNDIDYMDQSMDFTYDSKKFETLPDLVRDL-HAHNQTYVIMLDPGISSTQPEGSYWPFDEGLKRGVFIKDAE-GKTLIGKVWPGLTAYPDFSDEVTHDWWFDNLQKFHNKVPFDGLWIDMNEPSNFLDGSTNGCPSNS-LENPPYTPGVLGGLLRAKTVCATA-QQKLSMHYNLHSLYGLMEAKASASAL-KKLLTKRPFVISRSTFPSQGLYSGHWLGDNRSQWKDLYTSIAGILTFNLLGIPLVGADICGFSEEPQEELCVRWTQLGAFYPFTRNHNSIDMKPQDPTAFSPLARTAMKEALLLRYSLFPFLYTLFHHAHAHGHSVARPVMFEFPKDVKTYGIDKQFLWGRSLLVTPVLDPGVDYVVGYFPAGLWYDFYTGDSLHSKGEE--VRLQAPLDKINLHLREGSVIPTQTPNLTLWISTGQPLHLISALSDEGSAHGDLFWDDGESIDTFETNQYSYIVFSVA-QNTMTSQVLKSHQEASFITVGSASFYGVSAKP-SKVLVNSKDHPFTY 947
BLAST of Lysosomal alpha-glucosidase vs. Ensembl Medaka
Match: si:ch73-12o23.1 (lysosomal alpha-glucosidase [Source:NCBI gene;Acc:101169174]) HSP 1 Score: 712.605 bits (1838), Expect = 0.000e+0 Identity = 365/848 (43.04%), Postives = 508/848 (59.91%), Query Frame = 3
Query: 324 CQIANNNERLDCYPESGA--NQDSCIARGCCWSEPMRLSNKLQGVPLSIPYCYYPTDYSNYMIKSLRKSSRGIKAKLVRESPSYLPYEVAMLDLEVIYETKSRTRVRITDPKQKRYEVPMKLSPPDLKPPTDREYEVIIPSDGSFSIEIKRKSDDRSLFKS-FGPLLYSDQFLQISSIMSTDQIYGIGEQNRPFLHQLSNGWERIGLWARDNPIETFMNLYGAHPFYIGMPDDGKAFGVFHFNSNGQEFALQPAPAVTYRTIGGILDFFIFTGGSPEETVKQYLELVGKPMLPPYWSLGFHLSKWGYKNIEQLSEIFQRNINAGFPIGTQWSDIDYMENYKDFTVDKEKFNGLGRFVREIKQKYNSRFVAIVDPGISCTS-GNEYPPYYEGVFMDIFIKDSQTNQPIVGKVWPGETVYPDFTHPNISMYWYKQIQRFYNDVPIDGLWIDMNEPSNFYDGSKRGCQGNSKYDNPFFVPDIRDQSLISKTICSSALHYNNEKHYNLHNLYGYTESVTTNKALLKTIHGKRPFILSRSTFSGSGKYTFHWTGDNQASWDQLRNSIRQVLNFNMFGIPMTGADICGFNGNSDEELCIRWMQLGAFYPFMRNHNAINQRDQDPASWNTEAQKIMKTAISNRYAMLPLFYTLLVKSHMNGGTVMRPMSFEFFSDKVTHSIELQFMIGSSIMATPVVYPGARSVDAYFPAGVWYDFHSGKLLHSDAEGAWLTLDAPLDKINIALRGGHIVPLLDSNRNSKSNIPNKMTLYCGMAQDMSASGDLYWDDGESIDSYSRNRYSYVSFNASPEQLLITPVYFGYDLLP--VLNKIVILGFENKSITKFLLNDNDIPFHY 2849
C + R DCYPE G +D C AR CC+ SN Q +P+C+YP D+ +Y + S + G K LV+E +Y P ++ +++E ET +R VR+TDP R+EVP+ + P K + ++ S F I +KR+S L + PL Y+DQFLQ S+++ T IYG+GE FLH ++ W + +WARD P NLYG HPFY+ M +DG A G F NSN + LQP PA+T+RTIGGILDF++F G P V QYLE++G P +P YW+LG+HL +WGY + E+ + N G P TQW+DIDYM+ DFT D +KF L VR++ +N +V ++DPGIS T Y P+ EG+ +FIKD++ + ++GKVWPG T YPDF+ +W+ +Q+F+N VP DGLWIDMNEPSNF DGS GC NS +NP + P + L +KT+C++A HYNLH+LYG E+ + AL K + KRPF++SRSTF G Y+ HW GDN++ W L SI +L FN+ GIP+ GADICGF+ EELC+RW QLGAFYPF RNHN+I+ + QDP +++ A+ MK A+ RY++ P YTL +H +G +V RP+ FEF D T+ I+ QF+ G S++ TPV+ PG V YFPAG+WYDF++G LHS E + L APLDKIN+ LR G ++P N + + L ++ + SA GDL+WDDGESID++ N+YSY+ F+ + + + + V + + G K +K L+N D PF Y
Sbjct: 113 CSAISEPWRFDCYPERGVVVTKDMCEARNCCFIPASSSSNAGQSRKNGVPWCFYPPDFPSYSLVSTDDTLMGQKGTLVKEVKTYYPGDILTVEVETRQETDTRLHVRMTDPSNPRFEVPISVPNPTKK--AENPAYIVELSKQPFGITVKRRSTGVLLLNTTVAPLFYADQFLQFSTVLPTQFIYGLGEHRSTFLHDMN--WNTLTMWARDVPPMEKTNLYGVHPFYLAMEEDGNAHGFFLLNSNAMDVVLQPTPALTWRTIGGILDFYVFLGPDPGSVVSQYLEVIGNPAMPIYWALGYHLCRWGYNSSNSTWEVVKSLRNYGIPQDTQWNDIDYMDQSMDFTYDSKKFETLPDLVRDL-HAHNQTYVIMLDPGISSTQPEGSYWPFDEGLKRGVFIKDAE-GKTLIGKVWPGLTAYPDFSDEVTHDWWFDNLQKFHNKVPFDGLWIDMNEPSNFLDGSTNGCPSNS-LENPPYTPGVLGGLLRAKTVCATA-QQKLSMHYNLHSLYGLMEAKASASAL-KKLLTKRPFVISRSTFPSQGLYSGHWLGDNRSQWKDLYTSIAGILTFNLLGIPLVGADICGFSEEPQEELCVRWTQLGAFYPFTRNHNSIDMKPQDPTAFSPLARTAMKEALLLRYSLFPFLYTLFHHAHAHGHSVARPVMFEFPKDVKTYGIDKQFLWGRSLLVTPVLDPGVDYVVGYFPAGLWYDFYTGDSLHSKGEE--VRLQAPLDKINLHLREGSVIPTQTPNLTLWISTGQPLHLISALSDEGSAHGDLFWDDGESIDTFETNQYSYIVFSVA-QNTMTSQVLKSHQEASFITVGSASFYGVSAKP-SKVLVNSKDHPFTY 947
BLAST of Lysosomal alpha-glucosidase vs. Ensembl Medaka
Match: gaa (glucosidase alpha, acid [Source:NCBI gene;Acc:101161640]) HSP 1 Score: 656.751 bits (1693), Expect = 0.000e+0 Identity = 346/851 (40.66%), Postives = 494/851 (58.05%), Query Frame = 3
Query: 321 QCQIANNNERLDCYPESGANQDSCIARGCCWSEPMRLSNKLQGVPLSIPYCYYPTDYSNYMIKSLRKSSRGIKAKLVRESPSYLPYEVAMLDLEVIYETKSRTRVRITDPKQKRYEVPMKLSPPDLKPPTDREYEVIIPSDGSFSIEIKRKSDDRSLFKS-FGPLLYSDQFLQISSIMSTDQIYGIGEQNRPFLHQLSNGWERIGLWARDNPIETFMNLYGAHPFYIGMPDDGKAFGVFHFNSNGQEFALQPAPAVTYRTIGGILDFFIFTGGSPEETVKQYLELVGKPMLPPYWSLGFHLSKWGYKNIEQLSEIFQRNINAGFPIGTQWSDIDYMENYKDFTVDKEKFNGLGRFVREIKQKYNSRFVAIVDPGISCTSG-NEYPPYYEGVFMDIFIKDSQTNQPIVGKVWPGETVYPDFTHPNISMYWYKQIQRFYNDVPIDGLWIDMNEPSNFYDGSKRGCQGNSKYDNPFFVPDIRDQSLISKTICSSALHYNNEKHYNLHNLYGYTESVTTNKALLKTIHGKRPFILSRSTFSGSGKYTFHWTGDNQASWDQLRNSIRQVLNFNMFGIPMTGADICGFNGNSDEELCIRWMQLGAFYPFMRNHNAINQRDQDPASWNTEAQKIMKTAISNRYAMLPLFYTLLVKSHMNGGTVMRPMSFEFFSDKVTHSIELQFMIGSSIMATPVVYPGARSVDAYFPAGVWYDFHSGKLLHSDAEGAWLTLDAPLDKINIALRGGHIVPLLDSNRNSKSNIPNKMTLYCGMAQDMSASGDLYWDDGESIDSYSRNRYSYVSFNASPEQLLITPVYF--GYDLLPVLNKIVILGFENKSITKFLLNDNDIPFHYNPET 2861
+C I + R DC + +Q C RGCC+S P+ P+ P+C+YP Y Y + S+ G A L R PSYL E++ L LEV+ E+ + I DP +RYEVP+ K + F ++R ++ R + + PLL++DQ+LQ+S+ +++ + G+GE + L+ W + LW RD NLYG+HPFY+ + G A GVF NSN E LQP PA+T+ + GGILD ++F G P+ ++QYL+++G PM+PPYWSLGFHL +WGY + + Q +A FP+ QW+D+DY K FT D +F L V E ++ +++ I+DPGIS TS YPP+ +GV D+F+K++ + ++GKVWPG T +PDFT+ +W I+ FY++VP+DGLWIDMNEP++F GS GC N + P + P + L S T+C SA HYNLHNLYG TE+ T+ AL K I KRPF+LSRS+F G G+++ WTGD Q+ W+QL SI VL F++FG+P+ GADICGF GN+ EELC+RWMQLGAFYPFMRNHN Q+P + +AQ M+ A++ RY++LP YTL +H + TV RP+ EF SD SI+ QF+ G S++ +PV+ GA V AY P G WY ++G+ HS +G + L APLD INI +R GHI+P + + ++ L ++ D SA GDL+WDDGES+D++ YSY+ F A Q++ P+ D L VL + + G + I + + F Y +T
Sbjct: 71 ECTI-DPESRFDCGRDRLLSQGECEDRGCCFS-PLP-------SPVGPPWCFYPRWYPGYKMGPFSPSTHGKTASLTRAKPSYLFKEISPLTLEVMEESADCLHLTIKDPFTQRYEVPLPEGVSHTKADAQDVLFTVEFHSEPFGFIVRRATNGRVIMNTTVAPLLFADQYLQMSTTLASSFVSGLGEHYTSLVLDLN--WTSLTLWNRDMAPHADANLYGSHPFYMVQEEGGLAHGVFLLNSNAIEVILQPTPALTWISTGGILDLYVFMGPDPQSVIRQYLQVIGYPMMPPYWSLGFHLCRWGYTSSNATRSVAQHMHSANFPMDVQWNDLDYAHERKVFTFDPMRFGDLPEMVEEFHER-GMKYILILDPGISSTSPPRTYPPFEDGVKRDVFVKNAM-GEILIGKVWPGPTAFPDFTNVETRQWWEDCIRNFYSEVPVDGLWIDMNEPASFVQGSVEGCPDND-LERPPYTPRMVGGQLNSGTLCMSA-QQKLSTHYNLHNLYGLTEAYATHSAL-KKIQRKRPFVLSRSSFPGIGRFSAVWTGDVQSDWEQLGFSIPAVLQFSLFGVPLVGADICGFGGNTTEELCVRWMQLGAFYPFMRNHNDKPNAPQEPFVFGQKAQAAMRRAVNLRYSLLPFLYTLFHHAHTSAATVARPLFMEFPSDPNCKSIDQQFLWGGSLLISPVLKQGAVKVKAYLPLGTWYSLYNGQAFHS--KGQFFLLPAPLDTINIHVRQGHIIPQQEPALTTAASRRKPFFLIAALSGDGSARGDLFWDDGESLDTFEMQNYSYIIFTAVQFQIVSEPLKLNGALDGL-VLAGVQVFGVLSAPIYVLANGEKVLDFAYQADT 902
BLAST of Lysosomal alpha-glucosidase vs. Ensembl Medaka
Match: ENSORLT00000015395.2 (sucrase-isomaltase [Source:NCBI gene;Acc:101164831]) HSP 1 Score: 609.757 bits (1571), Expect = 0.000e+0 Identity = 326/820 (39.76%), Postives = 486/820 (59.27%), Query Frame = 3
Query: 324 CQIANNNERLDCYPESGANQDSCIARGCCWSEPMRLSNKLQGVPLSIPYCYYPTDYSNYMIKSLRKSS-RGIKAKLVR-ESPSYLPYEVAMLDLEVIYETKSRTRVRITDPKQKRYEVPMKLSPPDLKPPTDREYEVIIPSDGSFSIEIKRKSDDRSLF-KSFGPLLYSDQFLQISSIMSTDQIYGIGEQNRPFLHQLSNGWERIGLWARDN-PIETFMNLYGAHPFYIGMPDD-GKAFGVFHFNSNGQEFALQPAPAVTYRTIGGILDFFIFTGGSPEETVKQYLELVGKPMLPPYWSLGFHLSKWGYKNIEQLSEIFQRNINAGFPIGTQWSDIDYMENYKDFTVDKEKFNGLGRFVREIKQKYNSRFVAIVDPGISCTS--GN-EYPPYYEGVFMDIFIKDSQTNQPIVGKVWPGETVYPDFTHPNISMYWYKQIQRFYNDVPIDGLWIDMNEPSNFYDGSKRGCQGNSKYDNPFFVPDIRDQSLISKTICSSALH-YNNEKHYNLHNLYGYTESVTTNKALLKTIHGKRPFILSRSTFSGSGKYTFHWTGDNQASWDQLRNSIRQVLNFNMFGIPMTGADICGFNGNSDEELCIRWMQLGAFYPFMRNHNAINQRDQDPASWNTEAQKIM--KTAISNRYAMLPLFYTLLVKSHMNGGTVMRPMSFEFFSDKVTHSIELQFMIGSSIMATPVVYPGARSVDAYFPAGVWYDFHSGKLLHSDAEGAWLTLDAPLDKINIALRGGHIVPLLDSNRNSKSNIPNKMTLYCGMAQDMSASGDLYWDDGESIDSYSRNRYSYVSFNASPEQLLITPVYFGY 2750
C ER+DC+PE+GA+Q +C RGCCWS P+ N +P+C++ +++ Y S++ + I A+L R SPS ++ L +T +R R +I D +QKR+EVP + P+ + + ++ F + ++RK + + LF +F PL+++DQFLQ+S+ + + IYG+GE +N W +++RD P NLYG +PF++ + D+ G +FGVF NSN E LQPAPAVTYRTIGGILDFFIF G +PE+ V+++ EL+G+P++PPYWSLGF LS+W Y ++ ++ + +RN G P Q++DIDYME KDFT DK F L F + +K +++ I+DP I+ + G+ Y Y G + ++ +S +VG+VWPGETV+PD+T N +W + R Y +V D LWIDMNE +NF GS +GC N K + P + P I D + SKT+C A + N HY++H+LYGY+ + T +AL + G R +L+RS+F G GKY+ HW GDN A+W+ ++ +I +L F +FG+P GADICGF +S EELC RWMQ+GAFYPF RNHNA + QDPAS+ + + K +S RY +LP YTL K+H +G TV+RP+ EF+SD T +++ QF+ G ++ TPV+ PG SV YFP VWYD+ + + L L L P DK+ + +RGG I+P + + + + M L + + A+G+L+WDDG+S + +Y + F+ L + GY
Sbjct: 51 CPNVAQAERVDCFPETGASQLTCEQRGCCWS-PLDTPN--------VPWCFFSSNHG-YTAHSVKHPNPHEITAELSRMPSPSLFGADIQQLSFHAEMQTSNRLRFKIFDAQQKRFEVPHEHINTVTSNPSTPISDALEITNEPFGLIVRRKENKKVLFDTTFAPLVFADQFLQLSAKLPSHNIYGLGEHVHQNYRHDTN-WRTWPIFSRDAFPNGGTHNLYGHYPFFLCLEDNSGNSFGVFLLNSNAMEVTLQPAPAVTYRTIGGILDFFIFFGETPEQVVQEFEELIGRPVIPPYWSLGFQLSRWNYGSLSEVKKTVERNRAVGLPYDIQYTDIDYMEEKKDFTYDKVNFKDLPTFADYLHEK-GQKYILILDPAIATSKLIGDVAYGSYDRGTAKNAWVTESDGKTHLVGEVWPGETVFPDYTSQNCIDWWVDEYDRLYKEVKHDALWIDMNEVANFKQGSSKGCSVN-KLNYPPYTPRILDDLMYSKTLCMDAKQAWGN--HYDVHSLYGYSMVLATEQALQRVFGGNRTLMLTRSSFPGVGKYSGHWLGDNGANWNDIKWAIPGMLEFGLFGVPYIGADICGFFDDSSEELCRRWMQVGAFYPFSRNHNAEGYKPQDPASYGASSLLVATSKHYLSIRYTLLPYLYTLFYKAHTSGDTVVRPLLHEFYSDSDTWAVDRQFLWGKYLLITPVLDPGVESVQGYFPDAVWYDYETRERLGQRKTHVELFL--PADKLGLHIRGGAILPTQEPDVTTTHSRLRPMGLIIALDDNNQATGELFWDDGDSRATVENGKYIHYDFSVVDNVLAMDVTNAGY 853 HSP 2 Score: 592.423 bits (1526), Expect = 0.000e+0 Identity = 330/883 (37.37%), Postives = 502/883 (56.85%), Query Frame = 3
Query: 345 ERLDCYPESGANQDSCIARGCCWSEPMRLSNKLQGVPLSIPYCYYPTDYSNYMIKSLRKSSRGIKAKLVR----------ESPSYLPYEVAMLDLEVIYETKSRTRVRITDPKQKRYEVPMKLSPPDLKPPTD---REYEVIIPSDGSFSIEIKRKSDDRSLFKSFGP-LLYSDQFLQISSIMSTDQIYGIGE-QNRPFLHQLSNGWERIGLWARDNPIETFMNLYGAHPFYIGMPDDGKAFGVFHFNSNGQEFALQPAPAVTYRTIGGILDFFIFTGGSPEETVKQYLELVGKPMLPPYWSLGFHLSKWGYKNIEQLSEIFQRNINAGFPIGTQWSDIDYMENYKDFTVDKEKFNGLGRFVREIKQKYNSRFVAIVDPGISCTSGNEYPPYYEGVFMDIFIK-DSQTNQPIV-GKVWPG------------ET---------VYPDFTHPNISMYWYKQIQRFYNDV-PIDGLWIDMNEPSNFYDGSKRG-CQGNSKYDNPFFVPDI--RDQSLISKTICSSALHYNNE----KHYNLHNLYGYTESVTTNKALLKTIHGKRPFILSRSTFSGSGKYTFHWTGDNQASWDQLRNSIRQVLNFNMFGIPMTGADICGFNGNSDEELCIRWMQLGAFYPFMRNHNAINQRDQDPASWNTEAQKIMKTAISNRYAMLPLFYTLLVKSHMNGGTVMRPMSFEFFSDKVTHSIELQFMIGSSIMATPVVYPGARSVDAYFPAGVWYDFHSGKLLHSDAEGAWLTLDAPLDKINIALRGGHIVPLLDSNRNSKSNIPNKMTLYCGMAQDMSASGDLYWDDGESIDSYSRNRYSYVSFNASPEQLLITPVYFGY---DLLPVLNKIVILGFENKSITKFLLNDNDIPFH 2846
+R+DC+PE +N+ C RGC W ++P+C+YP DY Y + + +++ GI+ + R +SP ++ L +E+ Y T + +I DP +R+EVP+ L+ P P +D R Y V + ++ F I++ RKS +++ S P ++S+ F+QIS+ +S+ +YG GE ++ F H L + G++A+D P MN YG HPFY+G+ + G A GV NSN + QP P++TYRTIGGILDF++ G +PE V++Y LVG+P+LP YWSLGF L ++GY N ++ ++ AG P Q++DIDYME DF +D E F GL V +++ + RF+ I+DP IS N YP + GV D+FIK + IV GKVWP ET +PDF + +W+++IQ FY +V DGLWIDMNEP++F G+ G C G + + P ++P + +++ L KT+C ++ ++ KHY++HNLYG++ + T ALL + GKR +++RST+ SGK+ HW GDN +SWDQL SI ++ F++FGIP TGADICGF +D E+C+RWMQLGAFYP+ RNHN QDP +W+ I K ++ RY +LP YTL+ ++H G TV+RP+ EF +DK T I QF+ G +++ +P + G V+ Y P WYD+HS + + G WLT+ PL++IN+ +RGG+I+P N+K + N + L ++ +A G +WDDGE ID+ + +Y + +F+ + L V+ G D L L + + G + IT+ L D+ H
Sbjct: 931 QRVDCHPEENSNEAECKKRGCLWE---------VSAAENVPWCFYPDDYG-YNVLNYAETTTGIRVDINRNTRYRSSGRPDSP-----DIDSLRVEINYHTGDMLQFKIWDPSTERFEVPVPLTVPS-TPESDEDKRLYRVSV-TESPFGIQVVRKSTGTTVWDSSMPGFIFSNMFIQISTKLSSKFVYGFGETEHTSFKHDLD--YHTWGMFAKDQPPGYKMNCYGVHPFYMGLENTGDAHGVLLLNSNAMDVTFQPTPSLTYRTIGGILDFYMVLGPTPEMVVQEYTSLVGRPVLPAYWSLGFQLCRYGYANDSEIENLYTDMKKAGIPYDVQYADIDYMERQLDFVLDSE-FQGLPALVDQMRAE-GMRFIFILDPAISGNETN-YPAFERGVAQDVFIKWPKELGDGIVWGKVWPDFPNVTVDEDLDWETQVQIYRSYAAFPDFFRSQTAAWWHQEIQDFYTNVMKFDGLWIDMNEPASFVHGTVGGTCLGENLLEFPPYMPPLESKEEGLKHKTLCMNSEQILSDGKRVKHYDVHNLYGWSHTKPTYDALLD-VTGKRGIVVTRSTYPSSGKWAGHWLGDNNSSWDQLYKSIIGMMEFSLFGIPYTGADICGFFNEADYEMCLRWMQLGAFYPYSRNHNGKGNPRQDPVAWDDRFATISKDVLNIRYTLLPYLYTLMFEAHTQGSTVVRPVLHEFTNDKTTWDIHRQFLWGPALLISPALDAGQTVVNGYLPVARWYDYHSAQDV--GVRGKWLTMQTPLERINLHVRGGYILPWQKPESNTKLSRKNPLGLIVALSDSGTAEGSFFWDDGEGIDTVEQKKYLHATFSTASNTLTNKVVHNGLNPEDRL-TLGSVKVWGTGTEKITEVTLTDSTGKIH 1787
BLAST of Lysosomal alpha-glucosidase vs. Ensembl Medaka
Match: ENSORLT00000015393.2 (sucrase-isomaltase [Source:NCBI gene;Acc:101164831]) HSP 1 Score: 609.372 bits (1570), Expect = 0.000e+0 Identity = 326/820 (39.76%), Postives = 486/820 (59.27%), Query Frame = 3
Query: 324 CQIANNNERLDCYPESGANQDSCIARGCCWSEPMRLSNKLQGVPLSIPYCYYPTDYSNYMIKSLRKSS-RGIKAKLVR-ESPSYLPYEVAMLDLEVIYETKSRTRVRITDPKQKRYEVPMKLSPPDLKPPTDREYEVIIPSDGSFSIEIKRKSDDRSLF-KSFGPLLYSDQFLQISSIMSTDQIYGIGEQNRPFLHQLSNGWERIGLWARDN-PIETFMNLYGAHPFYIGMPDD-GKAFGVFHFNSNGQEFALQPAPAVTYRTIGGILDFFIFTGGSPEETVKQYLELVGKPMLPPYWSLGFHLSKWGYKNIEQLSEIFQRNINAGFPIGTQWSDIDYMENYKDFTVDKEKFNGLGRFVREIKQKYNSRFVAIVDPGISCTS--GN-EYPPYYEGVFMDIFIKDSQTNQPIVGKVWPGETVYPDFTHPNISMYWYKQIQRFYNDVPIDGLWIDMNEPSNFYDGSKRGCQGNSKYDNPFFVPDIRDQSLISKTICSSALH-YNNEKHYNLHNLYGYTESVTTNKALLKTIHGKRPFILSRSTFSGSGKYTFHWTGDNQASWDQLRNSIRQVLNFNMFGIPMTGADICGFNGNSDEELCIRWMQLGAFYPFMRNHNAINQRDQDPASWNTEAQKIM--KTAISNRYAMLPLFYTLLVKSHMNGGTVMRPMSFEFFSDKVTHSIELQFMIGSSIMATPVVYPGARSVDAYFPAGVWYDFHSGKLLHSDAEGAWLTLDAPLDKINIALRGGHIVPLLDSNRNSKSNIPNKMTLYCGMAQDMSASGDLYWDDGESIDSYSRNRYSYVSFNASPEQLLITPVYFGY 2750
C ER+DC+PE+GA+Q +C RGCCWS P+ N +P+C++ +++ Y S++ + I A+L R SPS ++ L +T +R R +I D +QKR+EVP + P+ + + ++ F + ++RK + + LF +F PL+++DQFLQ+S+ + + IYG+GE +N W +++RD P NLYG +PF++ + D+ G +FGVF NSN E LQPAPAVTYRTIGGILDFFIF G +PE+ V+++ EL+G+P++PPYWSLGF LS+W Y ++ ++ + +RN G P Q++DIDYME KDFT DK F L F + +K +++ I+DP I+ + G+ Y Y G + ++ +S +VG+VWPGETV+PD+T N +W + R Y +V D LWIDMNE +NF GS +GC N K + P + P I D + SKT+C A + N HY++H+LYGY+ + T +AL + G R +L+RS+F G GKY+ HW GDN A+W+ ++ +I +L F +FG+P GADICGF +S EELC RWMQ+GAFYPF RNHNA + QDPAS+ + + K +S RY +LP YTL K+H +G TV+RP+ EF+SD T +++ QF+ G ++ TPV+ PG SV YFP VWYD+ + + L L L P DK+ + +RGG I+P + + + + M L + + A+G+L+WDDG+S + +Y + F+ L + GY
Sbjct: 51 CPNVAQAERVDCFPETGASQLTCEQRGCCWS-PLDTPN--------VPWCFFSSNHG-YTAHSVKHPNPHEITAELSRMPSPSLFGADIQQLSFHAEMQTSNRLRFKIFDAQQKRFEVPHEHINTVTSNPSTPISDALEITNEPFGLIVRRKENKKVLFDTTFAPLVFADQFLQLSAKLPSHNIYGLGEHVHQNYRHDTN-WRTWPIFSRDAFPNGGTHNLYGHYPFFLCLEDNSGNSFGVFLLNSNAMEVTLQPAPAVTYRTIGGILDFFIFFGETPEQVVQEFEELIGRPVIPPYWSLGFQLSRWNYGSLSEVKKTVERNRAVGLPYDIQYTDIDYMEEKKDFTYDKVNFKDLPTFADYLHEK-GQKYILILDPAIATSKLIGDVAYGSYDRGTAKNAWVTESDGKTHLVGEVWPGETVFPDYTSQNCIDWWVDEYDRLYKEVKHDALWIDMNEVANFKQGSSKGCSVN-KLNYPPYTPRILDDLMYSKTLCMDAKQAWGN--HYDVHSLYGYSMVLATEQALQRVFGGNRTLMLTRSSFPGVGKYSGHWLGDNGANWNDIKWAIPGMLEFGLFGVPYIGADICGFFDDSSEELCRRWMQVGAFYPFSRNHNAEGYKPQDPASYGASSLLVATSKHYLSIRYTLLPYLYTLFYKAHTSGDTVVRPLLHEFYSDSDTWAVDRQFLWGKYLLITPVLDPGVESVQGYFPDAVWYDYETRERLGQRKTHVELFL--PADKLGLHIRGGAILPTQEPDVTTTHSRLRPMGLIIALDDNNQATGELFWDDGDSRATVENGKYIHYDFSVVDNVLAMDVTNAGY 853 HSP 2 Score: 592.423 bits (1526), Expect = 0.000e+0 Identity = 330/883 (37.37%), Postives = 502/883 (56.85%), Query Frame = 3
Query: 345 ERLDCYPESGANQDSCIARGCCWSEPMRLSNKLQGVPLSIPYCYYPTDYSNYMIKSLRKSSRGIKAKLVR----------ESPSYLPYEVAMLDLEVIYETKSRTRVRITDPKQKRYEVPMKLSPPDLKPPTD---REYEVIIPSDGSFSIEIKRKSDDRSLFKSFGP-LLYSDQFLQISSIMSTDQIYGIGE-QNRPFLHQLSNGWERIGLWARDNPIETFMNLYGAHPFYIGMPDDGKAFGVFHFNSNGQEFALQPAPAVTYRTIGGILDFFIFTGGSPEETVKQYLELVGKPMLPPYWSLGFHLSKWGYKNIEQLSEIFQRNINAGFPIGTQWSDIDYMENYKDFTVDKEKFNGLGRFVREIKQKYNSRFVAIVDPGISCTSGNEYPPYYEGVFMDIFIK-DSQTNQPIV-GKVWPG------------ET---------VYPDFTHPNISMYWYKQIQRFYNDV-PIDGLWIDMNEPSNFYDGSKRG-CQGNSKYDNPFFVPDI--RDQSLISKTICSSALHYNNE----KHYNLHNLYGYTESVTTNKALLKTIHGKRPFILSRSTFSGSGKYTFHWTGDNQASWDQLRNSIRQVLNFNMFGIPMTGADICGFNGNSDEELCIRWMQLGAFYPFMRNHNAINQRDQDPASWNTEAQKIMKTAISNRYAMLPLFYTLLVKSHMNGGTVMRPMSFEFFSDKVTHSIELQFMIGSSIMATPVVYPGARSVDAYFPAGVWYDFHSGKLLHSDAEGAWLTLDAPLDKINIALRGGHIVPLLDSNRNSKSNIPNKMTLYCGMAQDMSASGDLYWDDGESIDSYSRNRYSYVSFNASPEQLLITPVYFGY---DLLPVLNKIVILGFENKSITKFLLNDNDIPFH 2846
+R+DC+PE +N+ C RGC W ++P+C+YP DY Y + + +++ GI+ + R +SP ++ L +E+ Y T + +I DP +R+EVP+ L+ P P +D R Y V + ++ F I++ RKS +++ S P ++S+ F+QIS+ +S+ +YG GE ++ F H L + G++A+D P MN YG HPFY+G+ + G A GV NSN + QP P++TYRTIGGILDF++ G +PE V++Y LVG+P+LP YWSLGF L ++GY N ++ ++ AG P Q++DIDYME DF +D E F GL V +++ + RF+ I+DP IS N YP + GV D+FIK + IV GKVWP ET +PDF + +W+++IQ FY +V DGLWIDMNEP++F G+ G C G + + P ++P + +++ L KT+C ++ ++ KHY++HNLYG++ + T ALL + GKR +++RST+ SGK+ HW GDN +SWDQL SI ++ F++FGIP TGADICGF +D E+C+RWMQLGAFYP+ RNHN QDP +W+ I K ++ RY +LP YTL+ ++H G TV+RP+ EF +DK T I QF+ G +++ +P + G V+ Y P WYD+HS + + G WLT+ PL++IN+ +RGG+I+P N+K + N + L ++ +A G +WDDGE ID+ + +Y + +F+ + L V+ G D L L + + G + IT+ L D+ H
Sbjct: 931 QRVDCHPEENSNEAECKKRGCLWE---------VSAAENVPWCFYPDDYG-YNVLNYAETTTGIRVDINRNTRYRSSGRPDSP-----DIDSLRVEINYHTGDMLQFKIWDPSTERFEVPVPLTVPS-TPESDEDKRLYRVSV-TESPFGIQVVRKSTGTTVWDSSMPGFIFSNMFIQISTKLSSKFVYGFGETEHTSFKHDLD--YHTWGMFAKDQPPGYKMNCYGVHPFYMGLENTGDAHGVLLLNSNAMDVTFQPTPSLTYRTIGGILDFYMVLGPTPEMVVQEYTSLVGRPVLPAYWSLGFQLCRYGYANDSEIENLYTDMKKAGIPYDVQYADIDYMERQLDFVLDSE-FQGLPALVDQMRAE-GMRFIFILDPAISGNETN-YPAFERGVAQDVFIKWPKELGDGIVWGKVWPDFPNVTVDEDLDWETQVQIYRSYAAFPDFFRSQTAAWWHQEIQDFYTNVMKFDGLWIDMNEPASFVHGTVGGTCLGENLLEFPPYMPPLESKEEGLKHKTLCMNSEQILSDGKRVKHYDVHNLYGWSHTKPTYDALLD-VTGKRGIVVTRSTYPSSGKWAGHWLGDNNSSWDQLYKSIIGMMEFSLFGIPYTGADICGFFNEADYEMCLRWMQLGAFYPYSRNHNGKGNPRQDPVAWDDRFATISKDVLNIRYTLLPYLYTLMFEAHTQGSTVVRPVLHEFTNDKTTWDIHRQFLWGPALLISPALDAGQTVVNGYLPVARWYDYHSAQDV--GVRGKWLTMQTPLERINLHVRGGYILPWQKPESNTKLSRKNPLGLIVALSDSGTAEGSFFWDDGEGIDTVEQKKYLHATFSTASNTLTNKVVHNGLNPEDRL-TLGSVKVWGTGTEKITEVTLTDSTGKIH 1787
BLAST of Lysosomal alpha-glucosidase vs. Planmine SMEST
Match: SMESG000004892.1 (SMESG000004892.1) HSP 1 Score: 1592.79 bits (4123), Expect = 0.000e+0 Identity = 765/871 (87.83%), Postives = 818/871 (93.92%), Query Frame = 3
Query: 312 ATAQCQIANNNERLDCYPESGANQDSCIARGCCWSEPMRLSNKLQGVPLSIPYCYYPTDYSNYMIKSLRKSSRGIKAKLVRESPSYLPYEVAMLDLEVIYETKSRTRVRITDPKQKRYEVPMKLSPPDLKPPTDREYEVIIPSDGSFSIEIKRKSDDRSLFKSFGPLLYSDQFLQISSIMSTDQIYGIGEQNRPFLHQLSNGWERIGLWARDNPIETFMNLYGAHPFYIGMPDDGKAFGVFHFNSNGQEFALQPAPAVTYRTIGGILDFFIFTGGSPEETVKQYLELVGKPMLPPYWSLGFHLSKWGYKNIEQLSEIFQRNINAGFPIGTQWSDIDYMENYKDFTVDKEKFNGLGRFVREIKQKYNSRFVAIVDPGISCTSGNEYPPYYEGVFMDIFIKDSQTNQPIVGKVWPGETVYPDFTHPNISMYWYKQIQRFYNDVPIDGLWIDMNEPSNFYDGSKRGCQGNSKYDNPFFVPDIRDQSLISKTICSSALHYNNEKHYNLHNLYGYTESVTTNKALLKTIHGKRPFILSRSTFSGSGKYTFHWTGDNQASWDQLRNSIRQVLNFNMFGIPMTGADICGFNGNSDEELCIRWMQLGAFYPFMRNHNAINQRDQDPASWNTEAQKIMKTAISNRYAMLPLFYTLLVKSHMNGGTVMRPMSFEFFSDKVTHSIELQFMIGSSIMATPVVYPGARSVDAYFPAGVWYDFHSGKLLHSDAEGAWLTLDAPLDKINIALRGGHIVPLLDSNRNSKSNIPNKMTLYCGMAQDMSASGDLYWDDGESIDSYSRNRYSYVSFNASPEQLLITPVYFGYDLLPVLNKIVILGFENKSITKFLLNDNDIPFHYNPETXXXXXXXXXXXXXXEIKIQWM 2924
ATAQCQIANNNERLDCYPESGANQDSCI+RGCCWSEPMRLSNKLQGVPLSIPYCYYPTDYSNYMIKSLRKSSRGIKAKLVRESPSYLP EVAMLDLEVIYETKSRTRVRITDPKQKRYEVPMKLS DLKPPTDREYEVIIPSDGSFSIEIKRKSDDRSLFKSFGPLLYSDQFLQISSIMSTDQIYGIGEQNRPFLHQLSNGWERIGLWARD+P+ETF+NLYGAHPFYIGMP+DGKAFGVFHFNSNGQEFALQPAPAVTYRTIGG+LDFFIFTGGSPEETVKQYLELVGKPMLPPYWSLGFHLSK+GYK+I+++ E QRN+N+G + QWSD+DYM+ YKDF+VD +K+ G FV++I+ YN+R+V I+D IS + Y PY +G+ MDIFIK+ TN+PI+G+VWPG+TV+PDFTHP+I+ YW KQI+ F++ VP+DGLWIDMN+PS+F DGS GC N++YD PFFVP+IRDQSLI+KTICSS+LHYN EKHYNLHN+YG+ ESV TN LL+ KRPF+LSRSTFSGSGKY FHWTGDNQASWD LRNSIRQVLNFNMFGIPMTGADICGFNGNSDEELCIRWMQLGAFYPFMRNHNAINQRDQDPASWNTEAQKIMKTAISNRYAMLPLFYTLLVKSHMNGGTVMRPMSFEFFSDKVTHSIELQFMIGSSIMATPVVYPGARSVDAYFPAGVWYDFHSGKLLHSDAEGAWLTLDAPLDKINIALRGGHIVPLLDSNRNSKSNIPNKMTLYCGMAQDMSASGDLYWDDGESIDSYSRNRYSYVSFNASPEQLLITPVY GYDLLPVLNKIVILGF+NKSITKF+LNDNDIPFHYNPETSSVVLSSLNANLNSEIKIQWM
Sbjct: 21 ATAQCQIANNNERLDCYPESGANQDSCISRGCCWSEPMRLSNKLQGVPLSIPYCYYPTDYSNYMIKSLRKSSRGIKAKLVRESPSYLPNEVAMLDLEVIYETKSRTRVRITDPKQKRYEVPMKLSSQDLKPPTDREYEVIIPSDGSFSIEIKRKSDDRSLFKSFGPLLYSDQFLQISSIMSTDQIYGIGEQNRPFLHQLSNGWERIGLWARDHPVETFINLYGAHPFYIGMPEDGKAFGVFHFNSNGQEFALQPAPAVTYRTIGGVLDFFIFTGGSPEETVKQYLELVGKPMLPPYWSLGFHLSKYGYKDIKEIHETLQRNVNSGISVDVQWSDVDYMDGYKDFSVDNQKYQNFGEFVKQIRDTYNTRYVVIIDSAIS--NSENYIPYTDGLEMDIFIKNPDTNEPIIGEVWPGKTVFPDFTHPDITKYWSKQIRAFHDMVPVDGLWIDMNDPSSFVDGSTTGCSNNNRYDRPFFVPEIRDQSLIAKTICSSSLHYNKEKHYNLHNIYGFKESVVTNDVLLQMFPKKRPFLLSRSTFSGSGKYAFHWTGDNQASWDHLRNSIRQVLNFNMFGIPMTGADICGFNGNSDEELCIRWMQLGAFYPFMRNHNAINQRDQDPASWNTEAQKIMKTAISNRYAMLPLFYTLLVKSHMNGGTVMRPMSFEFFSDKVTHSIELQFMIGSSIMATPVVYPGARSVDAYFPAGVWYDFHSGKLLHSDAEGAWLTLDAPLDKINIALRGGHIVPLLDSNRNSKSNIPNKMTLYCGMAQDMSASGDLYWDDGESIDSYSRNRYSYVSFNASPEQLLITPVYLGYDLLPVLNKIVILGFDNKSITKFVLNDNDIPFHYNPETSSVVLSSLNANLNSEIKIQWM 889
BLAST of Lysosomal alpha-glucosidase vs. Planmine SMEST
Match: SMESG000005069.1 (SMESG000005069.1) HSP 1 Score: 1437.94 bits (3721), Expect = 0.000e+0 Identity = 676/869 (77.79%), Postives = 768/869 (88.38%), Query Frame = 3
Query: 315 TAQCQIANNNERLDCYPESGANQDSCIARGCCWSEPMRLSNKLQGVPLSIPYCYYPTDYSNYMIKSLRKSSRGIKAKLVRESPSYLPYEVAMLDLEVIYETKSRTRVRITDPKQKRYEVPMKLSPPDLKPPTDREYEVIIPSDGSFSIEIKRKSDDRSLFKSFGPLLYSDQFLQISSIMSTDQIYGIGEQNRPFLHQLSNGWERIGLWARDNPIETFMNLYGAHPFYIGMPDDGKAFGVFHFNSNGQEFALQPAPAVTYRTIGGILDFFIFTGGSPEETVKQYLELVGKPMLPPYWSLGFHLSKWGYKNIEQLSEIFQRNINAGFPIGTQWSDIDYMENYKDFTVDKEKFNGLGRFVREIKQKYNSRFVAIVDPGISCTSGNEYPPYYEGVFMDIFIKDSQTNQPIVGKVWPGETVYPDFTHPNISMYWYKQIQRFYNDVPIDGLWIDMNEPSNFYDGSKRGCQGNSKYDNPFFVPDIRDQSLISKTICSSALHYNNEKHYNLHNLYGYTESVTTNKALLKTIHGKRPFILSRSTFSGSGKYTFHWTGDNQASWDQLRNSIRQVLNFNMFGIPMTGADICGFNGNSDEELCIRWMQLGAFYPFMRNHNAINQRDQDPASWNTEAQKIMKTAISNRYAMLPLFYTLLVKSHMNGGTVMRPMSFEFFSDKVTHSIELQFMIGSSIMATPVVYPGARSVDAYFPAGVWYDFHSGKLLHSDAEGAWLTLDAPLDKINIALRGGHIVPLLDSNRNSKSNIPNKMTLYCGMAQDMSASGDLYWDDGESIDSYSRNRYSYVSFNASPEQLLITPVYFGYDLLPVLNKIVILGFENKSITKFLLNDNDIPFHYNPETXXXXXXXXXXXXXXEIKIQW 2921
+ QC I NNNERLDC+PE AN+ SC ++GCCWSEPMR SNKLQ +P SIPYCY+PT+Y NY +K + S+RGIKAKL+++ ++LP E+ L+L+VIYETKSR R+RI+D + KR+EVP+KL+P D PPTD +Y+VIIPSDGSFSIEIKRKSD RSLFK+FGPLLYSDQFLQIS++MSTDQ+YGIGEQNRPFLHQLSNGW RIGLWARD IETF NLYG HPFY+G+PDDGKAFGVFHFNSN QEF+LQPAPA+TYR+IGGILD FIFTG +PEETVKQY+ L+GKPMLPPYWSLGFHLSKWGYKNIEQLSEIFQRNINAGFPIGTQWSDIDYMENYKDFTVDKEKFNGLGRFVREIKQKYNSRFVAIVDPGISCTSGNEY PYYEGVFMDIFIKDS TNQPIVGKVWPGETVYPDFTHPNISMYWYKQIQRFYNDVPIDGLWIDMNEPSNFYDGSKRGCQGNSKYDNPFFVPDIRDQSLISKTICSSALHYNNEKHYNLHNLYGYTESVTTNKALLKTIHGKRPFILSRSTFSGSGKYTFHWTGDNQASWDQLRNSIRQVLNFNMFGIPMTGADICGFNGNS+EELCIRWMQLGAFYPFMRNHNAINQRDQDPASW+ EAQKIMK AI+ RYA+ P FYT LVKSH+ GGTVM+PMSFEF+SD+ TH ++LQFMIGSSIM PVV GAR V+AYFPAG+WYDF SG +L+SD++GA +TLDAPLDKIN ALRGGHIV L+ + + NKM LYC +++D ASGD YWDDGE++DSYS +Y+Y+ F A+ +L I+ + GY+L+PVL+K+VILG + + +ND + + ++ E S +++S+++ +++S I + W
Sbjct: 34 SPQCLIQNNNERLDCHPEPNANEQSCRSKGCCWSEPMRSSNKLQALPGSIPYCYFPTNYPNYAVKDIEVSNRGIKAKLIKQKFNHLPEEILSLNLDVIYETKSRVRIRISDTENKRFEVPIKLNPADPTPPTDTDYDVIIPSDGSFSIEIKRKSDGRSLFKTFGPLLYSDQFLQISTLMSTDQVYGIGEQNRPFLHQLSNGWTRIGLWARDYGIETFNNLYGTHPFYMGIPDDGKAFGVFHFNSNAQEFSLQPAPAITYRSIGGILDIFIFTGDTPEETVKQYVTLIGKPMLPPYWSLGFHLSKWGYKNIEQLSEIFQRNINAGFPIGTQWSDIDYMENYKDFTVDKEKFNGLGRFVREIKQKYNSRFVAIVDPGISCTSGNEYSPYYEGVFMDIFIKDSHTNQPIVGKVWPGETVYPDFTHPNISMYWYKQIQRFYNDVPIDGLWIDMNEPSNFYDGSKRGCQGNSKYDNPFFVPDIRDQSLISKTICSSALHYNNEKHYNLHNLYGYTESVTTNKALLKTIHGKRPFILSRSTFSGSGKYTFHWTGDNQASWDQLRNSIRQVLNFNMFGIPMTGADICGFNGNSNEELCIRWMQLGAFYPFMRNHNAINQRDQDPASWSIEAQKIMKIAINIRYALFPHFYTSLVKSHLYGGTVMKPMSFEFYSDRNTHGLDLQFMIGSSIMVVPVVDQGARKVNAYFPAGIWYDFQSGSILYSDSKGAQVTLDAPLDKINFALRGGHIVSLISDSMTNPQMSTNKMILYCALSEDSQASGDAYWDDGENVDSYSGKKYNYLKFIANGNRLEISVEFKGYELVPVLSKVVILGVKQTGVQNVSINDRPVDYQFDAEKSILLISNIDLSIDSHILLSW 902
BLAST of Lysosomal alpha-glucosidase vs. Planmine SMEST
Match: SMESG000005050.1 (SMESG000005050.1) HSP 1 Score: 1134.78 bits (2934), Expect = 0.000e+0 Identity = 530/841 (63.02%), Postives = 648/841 (77.05%), Query Frame = 3
Query: 321 QCQIANNNERLDCYPESGANQDSCIARGCCWSEPMRLSNKLQGVPLSIPYCYYPTDYSNYMIKSLRKSSRGIKAKLVRESPSYLPYEVAMLDLEVIYETKSRTRVRITDPKQKRYEVPMKLSPPDLKPPTDREYEVIIPSDGSFSIEIKRKSDDRSLFKSFGPLLYSDQFLQISSIMSTDQIYGIGEQNRPFLHQLSNGWERIGLWARDNPIETFMNLYGAHPFYIGMPDDGKAFGVFHFNSNGQEFALQPAPAVTYRTIGGILDFFIFTGGSPEETVKQYLELVGKPMLPPYWSLGFHLSKWGYKNIEQLSEIFQRNINAGFPIGTQWSDIDYMENYKDFTVDKEKFNGLGRFVREIKQKYNSRFVAIVDPGISCTSGNEYPPYYEGVFMDIFIKDSQTNQPIVGKVWPGETVYPDFTHPNISMYWYKQIQRFYNDVPIDGLWIDMNEPSNFYDGSKRGCQGNSKYDNPFFVPDIRDQSLISKTICSSALHYNNEKHYNLHNLYGYTESVTTNKALLKTIHGKRPFILSRSTFSGSGKYTFHWTGDNQASWDQLRNSIRQVLNFNMFGIPMTGADICGFNGNSDEELCIRWMQLGAFYPFMRNHNAINQRDQDPASWNTEAQKIMKTAISNRYAMLPLFYTLLVKSHMNGGTVMRPMSFEFFSDKVTHSIELQFMIGSSIMATPVVYPGARSVDAYFPAGVWYDFHSGKLLHSDAEGAWLTLDAPLDKINIALRGGHIVPLLDSNRNSKSNIPNKMTLYCGMAQDMSASGDLYWDDGESIDSYSRNRYSYVSFNASPEQLLITPVYFGYDLLPVLNKIVILGFENKSITKFLLNDNDIPF 2843
QC + NNN RLDC+P S ANQ+ C+ARGCCWS P Q P +IPYC++P +Y NY I S + RG KA+L ++ S+L E+ L+ EVI+ET +R R+RITDP +R+EVP++L PD PP++ +Y+ +P++GSF E+KR SD SLFK+FGPLLYSDQF+QISS+MS +YGIGEQNRPFLH LS WERIGLWARDN I+T+ NLYG HPFY+ +PD G+AFGV++FNSN QEF LQPAPA+TYRTIGGILD FIF+GG+PE TV QY EL+G+PMLPP+WSLGFHLSK+GYK+I +S RNIN PI QWSDIDYM+ YKDFT+D ++ L FV+ + Q R++ I+DPGIS S Y Y EG+ M+IFIKD+ T +P++G+VWPGET YPDFTHPN S YW QIQRF++ VPIDGLWIDMNEPSNFYDGS+ GC N+ YD PFFVP IRD SLI KTICSS+LHYNN HYNLHNLYGYTE+V T+ ALL TI KRPFILSRSTFSGSGKYTFHWTGDN A+WDQL NSIRQVLNFNMFGIPMTGADICGFNG+S+EELCIRWMQLGAFYPFMRNHN+I + QDPASW+ EAQ IMK +I RY LP FYTLLVKS++ GGTVMRP+SF+F+SD TH IE QFMIGSS++ PVV GAR+V AYFPAGVWYD ++ +++HS G W++LDAP+DK+N A+RGG I+ L + S+ LYC ++ + +ASG+LYWDDGES DSYS N+YSY++F A L +TP + G+ +P+L I ++G EN SITK LN ++PF
Sbjct: 20 QCSVPNNNYRLDCFPGSNANQNDCLARGCCWSPP-ETREYSQNNP-TIPYCFFPIEYPNYQINSFTPTDRGFKARLTKQQKSFLRKEILNLNFEVIFETNTRLRIRITDPNVQRFEVPIQLGSPDPTPPSNIQYKFTMPTNGSFGFEVKRNSDGLSLFKTFGPLLYSDQFIQISSLMSIQHVYGIGEQNRPFLHDLSTQWERIGLWARDNGIDTYNNLYGTHPFYMSLPDSGEAFGVYYFNSNAQEFVLQPAPAITYRTIGGILDIFIFSGGTPETTVSQYTELIGRPMLPPFWSLGFHLSKYGYKSIADVSNTLYRNINISIPIEVQWSDIDYMDKYKDFTLDPINYSKLSSFVKYLNQSLGIRYIVIIDPGISIQSPGAYSAYDEGLEMNIFIKDANTKKPLIGQVWPGETCYPDFTHPNASKYWSHQIQRFHSQVPIDGLWIDMNEPSNFYDGSRTGCALNNPYDYPFFVPTIRDGSLIKKTICSSSLHYNNVMHYNLHNLYGYTEAVATHSALLNTIPNKRPFILSRSTFSGSGKYTFHWTGDNSANWDQLANSIRQVLNFNMFGIPMTGADICGFNGDSNEELCIRWMQLGAFYPFMRNHNSIGAKAQDPASWSQEAQIIMKKSIIIRYVWLPNFYTLLVKSYLYGGTVMRPLSFQFYSDAETHEIERQFMIGSSMLVNPVVVQGARNVVAYFPAGVWYDGYTNQVVHSGPIGKWISLDAPIDKLNYAIRGGSILTGLFPAMTTTSSRNGPCFLYCALSLNRTASGELYWDDGESFDSYSGNKYSYITFTADSRGLRVTPQFVGFRSIPLLASISVVGLENLSITKVSLNGKEVPF 858
BLAST of Lysosomal alpha-glucosidase vs. Planmine SMEST
Match: SMESG000005054.1 (SMESG000005054.1) HSP 1 Score: 1117.06 bits (2888), Expect = 0.000e+0 Identity = 522/842 (62.00%), Postives = 635/842 (75.42%), Query Frame = 3
Query: 321 QCQIANNNERLDCYPESGANQDSCIARGCCWSEPMRLSNKLQGVPLSIPYCYYPTDYSNYMIKSLRKSSRGIKAKLVRESPSYLPYEVAMLDLEVIYETKSRTRVRITDPKQKRYEVPMKLSPPDLKPPTDREYEVIIPSDGSFSIEIKRKSDDRSLFKSFGPLLYSDQFLQISSIMSTDQIYGIGEQNRPFLHQLSNGWERIGLWARDNPIETFMNLYGAHPFYIGMPDDGKAFGVFHFNSNGQEFALQPAPAVTYRTIGGILDFFIFTGGSPEETVKQYLELVGKPMLPPYWSLGFHLSKWGYKNIEQLSEIFQRNINAGFPIGTQWSDIDYMENYKDFTVDKEKFNGLGRFVREIKQKYNSRFVAIVDPGISCTSGNEYPPYYEGVFMDIFIKDSQTNQPIVGKVWPGETVYPDFTHPNISMYWYKQIQRFYNDVPIDGLWIDMNEPSNFYDGSKRGCQGNSKYDNPFFVPDIRDQSLISKTICSSALHYNNEKHYNLHNLYGYTESVTTNKALLKTIHGKRPFILSRSTFSGSGKYTFHWTGDNQASWDQLRNSIRQVLNFNMFGIPMTGADICGFNGNSDEELCIRWMQLGAFYPFMRNHNAINQRDQDPASWNTEAQKIMKTAISNRYAMLPLFYTLLVKSHMNGGTVMRPMSFEFFSDKVTHSIELQFMIGSSIMATPVVYPGARSVDAYFPAGVWYDFHSGKLLHSDAEGAWLTLDAPLDKINIALRGGHIVPLLDSNRNSKSNIPNKMTLYCGMAQDMSASGDLYWDDGESIDSYSRNRYSYVSFNASPEQLLITPVYFGYDLLPVLNKIVILGFENKSITKFLLNDNDIPFH 2846
QC + NNN RLDC+P S ANQ+ C+ARGCCWS P Q P +IPYC++P +Y NY I S + RG KA+L ++ S+L E+ L+ EVI+ET +R R+RITDP +R+EVP++L PD PP++ +Y+ +P +GSF E+KR SD SLFK+FGPLLYSDQF+QISS+MS +YGIGEQNRPFLH LS WERIGLWARDNP++T+ NLYG HPFY+ +PD G+AFGV++FNSN QEF LQPAPA+TYRTIGGILD FIF+GG+PE TV QY EL+G+PMLPPYWSLGFHLSK+GYK+I +S RNIN PI QWSDIDYM+ YKDFT D K+ G FV + R++ I+DPGIS S Y PY EG+ M+++IKD+ T +P++ KVWPGET +PDFTHPN S YW QIQRF++ VPIDGLWIDMNEPSNFY GSK GC GNS YD P+F P IRD SLI TIC S+LHYNN HYNLHNLYGYTE+V T+ ALL TI KRPFILSRSTFSGSGKYTFHWTGDN A+WDQL NSIRQVLNFNMFGIPMTGADICGF G+S+EELCIRWMQLGAFYPFMRNHN + RDQDPA+W+ AQ IM+ +I+ RYA+LP YTLLVKSH+ GGTVMRP+SFEF+ D T IE QFMIGS+I+ PVV GAR+V AYFPAG+WYD +++S G W++L+APL++ N+ALRGGH + +L S NS + L C ++ + +A+GDLYWDDGES+DSYS ++SY F A+ +LI P GY +LP LN I+ILG SIT +ND I F+
Sbjct: 19 QCSVPNNNYRLDCFPGSNANQNDCLARGCCWSPP-ETREYSQNNP-TIPYCFFPIEYPNYQINSFTPTVRGFKARLTKQQKSFLRKEILNLNFEVIFETNTRLRIRITDPNVQRFEVPIQLGSPDPTPPSNVQYKFTMPRNGSFGFEVKRNSDGLSLFKTFGPLLYSDQFIQISSLMSIQHVYGIGEQNRPFLHDLSTQWERIGLWARDNPVDTYNNLYGTHPFYMSLPDSGEAFGVYYFNSNAQEFVLQPAPAITYRTIGGILDIFIFSGGTPETTVSQYTELIGRPMLPPYWSLGFHLSKYGYKSIADVSNTLYRNINISIPIEVQWSDIDYMDKYKDFTSDPIKYANFGSFVAYLNITLGIRYIVIIDPGISIQSPGAYSPYDEGLEMNLYIKDANTKKPLIAKVWPGETCFPDFTHPNASKYWSNQIQRFHSQVPIDGLWIDMNEPSNFYYGSKTGCPGNSPYDQPYFTPLIRDNSLIKSTICLSSLHYNNVMHYNLHNLYGYTEAVATHSALLNTIPNKRPFILSRSTFSGSGKYTFHWTGDNSANWDQLANSIRQVLNFNMFGIPMTGADICGFLGDSNEELCIRWMQLGAFYPFMRNHNTLGVRDQDPAAWSKGAQDIMRQSINIRYALLPNLYTLLVKSHLYGGTVMRPLSFEFYGDAETLEIERQFMIGSAILVNPVVVQGARNVVAYFPAGIWYDATDLSVIYSGKTGKWISLNAPLERTNVALRGGHAL-ILSSLVNSTEYSRKVINLVCILSMNQTAAGDLYWDDGESMDSYSGKKFSYFKFTATSSSVLINPEILGYSVLPSLNSIIILGINTSSITTVTINDKPIQFY 857
BLAST of Lysosomal alpha-glucosidase vs. Planmine SMEST
Match: SMESG000005053.1 (SMESG000005053.1) HSP 1 Score: 1091.64 bits (2822), Expect = 0.000e+0 Identity = 509/841 (60.52%), Postives = 633/841 (75.27%), Query Frame = 3
Query: 321 QCQIANNNERLDCYPESGANQDSCIARGCCWSEPMRLSNKLQGVPLSIPYCYYPTDYSNYMIKSLRKSSRGIKAKLVRESPSYLPYEVAMLDLEVIYETKSRTRVRITDPKQKRYEVPMKLSPPDLKPPTDREYEVIIPSDGSFSIEIKRKSDDRSLFKSFGPLLYSDQFLQISSIMSTDQIYGIGEQNRPFLHQLSNGWERIGLWARDNPIETFMNLYGAHPFYIGMPDDGKAFGVFHFNSNGQEFALQPAPAVTYRTIGGILDFFIFTGGSPEETVKQYLELVGKPMLPPYWSLGFHLSKWGYKNIEQLSEIFQRNINAGFPIGTQWSDIDYMENYKDFTVDKEKFNGLGRFVREIKQKYNSRFVAIVDPGISCTSGNEYPPYYEGVFMDIFIKDSQTNQPIVGKVWPGETVYPDFTHPNISMYWYKQIQRFYNDVPIDGLWIDMNEPSNFYDGSKRGCQGNSKYDNPFFVPDIRDQSLISKTICSSALHYNNEKHYNLHNLYGYTESVTTNKALLKTIHGKRPFILSRSTFSGSGKYTFHWTGDNQASWDQLRNSIRQVLNFNMFGIPMTGADICGFNGNSDEELCIRWMQLGAFYPFMRNHNAINQRDQDPASWNTEAQKIMKTAISNRYAMLPLFYTLLVKSHMNGGTVMRPMSFEFFSDKVTHSIELQFMIGSSIMATPVVYPGARSVDAYFPAGVWYDFHSGKLLHSDAEGAWLTLDAPLDKINIALRGGHIVPLLDSNRNSKSNIPNKMTLYCGMAQDMSASGDLYWDDGESIDSYSRNRYSYVSFNASPEQLLITPVYFGYDLLPVLNKIVILGFENKSITKFLLNDNDIPF 2843
QC + NNN RLDC+P + +NQ+ C+ARGCCWS P Q P +IPYC++P +Y NY I S + RG KA+L ++ S+L E+ L+ EVI+ET +R R+RITDP +R+EVP++L PD PP++ +Y+ +P++GSF E+KR SD SLFK+FGPLLYSDQF+QISS+MS +YGIGEQNRPFLH LS WERIGLW+RD I+T+ NLYG HPFY+ +PD G+AFGV++FNSN QEF LQPAPA+TYRTIGGILD FIF+GG+PE TV QY EL+G+PMLPP+WSLGFHLSK+G NI +S RNIN PI QWSDIDYM+ YKDFT D ++ G FV + Q +++ I+DPGIS S Y Y EG+ ++IFIKD+ T + ++ VWPGET +PDFTHPN S YW QIQRF++ VPIDGLWIDMNEP++FY GS GC GN+ YD PFFVP IRD SLI+KTICSS+LHYNN HYNLHNLYGYTE+V T+ ALL TI KRPFILSRSTFSGSGKYTFHWTGDN + WDQL NSIRQ+LNFNMFG+PM G DICGFN N +EELCIRWMQLGAFYPFMRNHN RDQDPASW+ EAQ IM+ +I+ RY LP FYTLLVKS++ GGTVMRP+SF+F+ D T IE QFMIGSS++ PVV GAR+V AYFP+GVWYD ++ +++HS G W++LDAP+DK+N A+RGG I+ L + S+ LYC ++ + +ASG+LYWDDGES DSYS N+YSY++F A L +TP + G+ +P+L I ++G EN SITK LN ++PF
Sbjct: 20 QCSVLNNNYRLDCFPGTNSNQNDCLARGCCWSPP-ETREYSQNNP-TIPYCFFPIEYPNYQINSFTPTDRGFKARLTKQQKSFLRKEILNLNFEVIFETNTRLRIRITDPNVQRFEVPIQLGSPDPTPPSNIQYKFTMPTNGSFGFEVKRNSDGLSLFKTFGPLLYSDQFIQISSLMSIQHVYGIGEQNRPFLHDLSTQWERIGLWSRDYGIDTYNNLYGTHPFYMSLPDSGEAFGVYYFNSNAQEFVLQPAPAITYRTIGGILDIFIFSGGTPETTVSQYTELIGRPMLPPFWSLGFHLSKYGQNNIADVSNTLYRNINISIPIEVQWSDIDYMDKYKDFTSDPIRYPNFGSFVAHLNQSLGIKYIVIIDPGISIQSPGVYSAYDEGLELNIFIKDANTKKDLISNVWPGETCFPDFTHPNASKYWSHQIQRFHSQVPIDGLWIDMNEPASFYYGSHIGCPGNNPYDYPFFVPTIRDSSLITKTICSSSLHYNNVMHYNLHNLYGYTEAVATHSALLNTIPNKRPFILSRSTFSGSGKYTFHWTGDNWSHWDQLANSIRQLLNFNMFGMPMVGEDICGFNDNCNEELCIRWMQLGAFYPFMRNHNTYGVRDQDPASWSKEAQNIMRKSINLRYVWLPNFYTLLVKSYLYGGTVMRPLSFQFYDDVETREIERQFMIGSSMLVNPVVVQGARNVVAYFPSGVWYDGYTNQVVHSGPIGKWISLDAPIDKLNYAIRGGSILTGLFPAMTTTSSRNGPCFLYCALSLNRTASGELYWDDGESFDSYSGNKYSYITFTADSRGLKVTPQFVGFRSIPLLASISVVGLENLSITKVSLNGKEVPF 858 The following BLAST results are available for this feature:
BLAST of Lysosomal alpha-glucosidase vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Lysosomal alpha-glucosidase vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of Lysosomal alpha-glucosidase vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of Lysosomal alpha-glucosidase vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of Lysosomal alpha-glucosidase vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of Lysosomal alpha-glucosidase vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of Lysosomal alpha-glucosidase vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Lysosomal alpha-glucosidase vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Lysosomal alpha-glucosidase vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of Lysosomal alpha-glucosidase vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of Lysosomal alpha-glucosidase vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 1
BLAST of Lysosomal alpha-glucosidase vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 3
BLAST of Lysosomal alpha-glucosidase vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of Lysosomal alpha-glucosidase vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30028442 ID=SMED30028442|Name=Lysosomal alpha-glucosidase|organism=Schmidtea mediterranea sexual|type=transcript|length=2946bpback to top protein sequence of SMED30028442-orf-1 >SMED30028442-orf-1 ID=SMED30028442-orf-1|Name=SMED30028442-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=892bp MKLIILISFINLISLFNFSNATAQCQIANNNERLDCYPESGANQDSCIARback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
|
