SMED30028360

Overview
NameSMED30028360
Smed IDSMED30028360
Length (bp)389
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30028360 (SMED30028360) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30028360 (SMED30028360) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30028360 (SMED30028360) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30028360 (SMED30028360) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30028360

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 33

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
Smed sexual biotypeSMED30028360h1SMcG0000165 Contig3847GPL15192PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30028360h1SMcG0018755 Contig3847GPL15192PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30028360h1SMcG0019025 Contig3847GPL15192PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30028360h1SMcG0018950 Contig3847GPL15192PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30028360h1SMcG0019222 Contig3847GPL15192PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30028360h1SMcG0019026 Contig3847GPL15192PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30028360h1SMcG0018731 Contig3847GPL15192PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30028360h1SMcG0018842 Contig3847GPL15192PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30028360h1SMcG0018747 Contig3847GPL15192PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30028360h1SMcG0018736 Contig3847GPL15192PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30028360h1SMcG0018744 Contig3847GPL15192PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30028360h1SMcG0018791 Contig3847GPL15192PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30028360h1SMcG0018862 Contig3847GPL15192PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30028360h1SMcG0018903 Contig3847GPL15192PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30028360h1SMcG0018729 Contig3847GPL15192PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
protonephridiaSMED30028360h1SMcG0018851 dd_Smed_v4_1139_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30028360h1SMcG0018851 dd_Smed_v4_1139_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30028360h1SMcG0018851 dd_Smed_v4_1139_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30028360h1SMcG0018851 dd_Smed_v4_1139_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30028360h1SMcG0018851 dd_Smed_v4_1139_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30028360h1SMcG0018851 dd_Smed_v4_1139_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30028360h1SMcG0018851 dd_Smed_v4_1139_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30028360h1SMcG0018851 dd_Smed_v4_1139_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
protonephridiaSMED30028360h1SMcG0018744 dd_Smed_v4_1139_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30028360h1SMcG0018744 dd_Smed_v4_1139_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30028360h1SMcG0018744 dd_Smed_v4_1139_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30028360h1SMcG0018744 dd_Smed_v4_1139_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30028360h1SMcG0018744 dd_Smed_v4_1139_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30028360h1SMcG0018744 dd_Smed_v4_1139_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30028360h1SMcG0018744 dd_Smed_v4_1139_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30028360h1SMcG0018744 dd_Smed_v4_1139_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30028360h1SMcG0018851 dd_Smed_v4_1139_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
epidermisSMED30028360h1SMcG0018744 dd_Smed_v4_1139_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
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BLAST of SMED30028360 vs. TrEMBL
Match: A0A0F7GWW3 (TNF receptor associated factor 4 (Fragment) OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 82.4185 bits (202), Expect = 4.060e-17
Identity = 36/46 (78.26%), Postives = 43/46 (93.48%), Query Frame = 1 
Query:  250 INQ*ISRLKVKCSEKSCEFVDELRKMEEHSRTCKYRSVACPYSNIG 387
            +N  I++L+VKC EKSC++VDELRKME+HSRTCKYRSVACPYSNIG
Sbjct:   22 MNMKIAKLQVKCKEKSCDYVDELRKMEDHSRTCKYRSVACPYSNIG 67
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BLAST of SMED30028360 vs. TrEMBL
Match: I1ZID8 (TNF receptor associated factor-2 (Fragment) OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 52.373 bits (124), Expect = 9.567e-11
Identity = 38/87 (43.68%), Postives = 52/87 (59.77%), Query Frame = 3 
Query:   12 ENVGQIMRDYNYESVTEIQHDMXXXXXXXXXXXARCVPCGCRFCFSCISGFLDGGNKICPGFTEECKLEILNLDKNIYVDQPMNIKI 272
            E + +++  YN+E    +  ++ C LCKNLL+NA    CGCRFC  CI  ++  GN  CPG T ECK E L +  NI+ D  +N KI
Sbjct:    4 EKINRLLITYNFEVEGGVPDNLKCDLCKNLLVNACQSRCGCRFCSKCIMEYVSRGNLYCPGGTSECKEENLTICSNIHTDYRVNRKI 90

HSP 2 Score: 41.9726 bits (97), Expect = 9.567e-11
Identity = 18/46 (39.13%), Postives = 31/46 (67.39%), Query Frame = 1 
Query:  250 INQ*ISRLKVKCSEKSCEFVDELRKMEEHSRTCKYRSVACPYSNIG 387
            +N+ I++++VKC E++C+   EL  +EEH R C  +S  CP+  +G
Sbjct:   86 VNRKIAKMRVKCPEETCKVYCELVDIEEHLRLCTMKSQNCPFFVLG 131
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BLAST of SMED30028360 vs. TrEMBL
Match: T1E101 (TRAF-6 OS=Dendrocoelum lacteum OX=27895 PE=2 SV=1)

HSP 1 Score: 52.373 bits (124), Expect = 2.716e-9
Identity = 33/91 (36.26%), Postives = 58/91 (63.74%), Query Frame = 3 
Query:    3 SKLENVGQIMRDYNYESVTE-IQHDMXXXXXXXXXXXARCVPCGCRFCFSCISGFLDGGNKICPGFTEECKLEILNLDKNIYVDQPMNIKI 272
            SKL+NV +++  YN + V E + +D+LC+LC  L+ +A    CGC +C++CI  +L   +  CPG   ECK + LNL+ +++ +  ++ +I
Sbjct:    4 SKLDNVQELLSKYNVKRVDESVNNDLLCILCNKLMRDACESACGCHYCYTCIRIYLSQNHNYCPGDKTECKEDYLNLEGDVFRNIRLSTRI 94

HSP 2 Score: 36.5798 bits (83), Expect = 2.716e-9
Identity = 18/42 (42.86%), Postives = 23/42 (54.76%), Query Frame = 1 
Query:  262 ISRLKVKCSEKSCEFVDELRKMEEHSRTCKYRSVACPYSNIG 387
            ISR+ VKC    C +  EL +++EH   C   SV CP   IG
Sbjct:   94 ISRIVVKCPNIRCNYTSELLRLKEHLDKCTPESVECPCVTIG 135
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BLAST of SMED30028360 vs. Planmine SMEST
Match: SMESG000025558.1 (SMESG000025558.1)

HSP 1 Score: 150.214 bits (378), Expect = 1.255e-55
Identity = 85/90 (94.44%), Postives = 86/90 (95.56%), Query Frame = 3 
Query:    3 SKLENVGQIMRDYNYESVTEIQHDMXXXXXXXXXXXARCVPCGCRFCFSCISGFLDGGNKICPGFTEECKLEILNLDKNIYVDQPMNIKI 272
            SK EN+ QIMR  NYESVTEIQHDMLCLLCKNLLLNARCVPCGCRFCFSCISGFLDGGNKICPGFTEECKLEILNLDKNIYVDQPMNIKI
Sbjct:    4 SKFENLWQIMR--NYESVTEIQHDMLCLLCKNLLLNARCVPCGCRFCFSCISGFLDGGNKICPGFTEECKLEILNLDKNIYVDQPMNIKI 91

HSP 2 Score: 83.1889 bits (204), Expect = 1.255e-55
Identity = 38/42 (90.48%), Postives = 39/42 (92.86%), Query Frame = 1 
Query:  262 ISRLKVKCSEKSCEFVDELRKMEEHSRTCKYRSVACPYSNIG 387
            ISRLKVKC EKSCEFVDELRKME+HSR CKYRS ACPYSNIG
Sbjct:   91 ISRLKVKCPEKSCEFVDELRKMEDHSRMCKYRSAACPYSNIG 132
[Close]
BLAST of SMED30028360 vs. Planmine SMEST
Match: SMESG000025583.1 (SMESG000025583.1)

HSP 1 Score: 138.658 bits (348), Expect = 3.764e-52
Identity = 77/90 (85.56%), Postives = 80/90 (88.89%), Query Frame = 3 
Query:    3 SKLENVGQIMRDYNYESVTEIQHDMXXXXXXXXXXXARCVPCGCRFCFSCISGFLDGGNKICPGFTEECKLEILNLDKNIYVDQPMNIKI 272
            SK EN+ QIMR  NYESVTEIQHDMLCLLCK LL+NA C  CGCRFCFSCISGFLDG NKICPGFTEECKLEI NLD+NIYVDQPMNIKI
Sbjct:    4 SKFENLWQIMR--NYESVTEIQHDMLCLLCKKLLVNAHCAQCGCRFCFSCISGFLDGENKICPGFTEECKLEIFNLDENIYVDQPMNIKI 91

HSP 2 Score: 82.8037 bits (203), Expect = 3.764e-52
Identity = 38/42 (90.48%), Postives = 39/42 (92.86%), Query Frame = 1 
Query:  262 ISRLKVKCSEKSCEFVDELRKMEEHSRTCKYRSVACPYSNIG 387
            ISRLKVKC EKSCEFVDELRKME+HSR CKYRS ACPYSNIG
Sbjct:   91 ISRLKVKCHEKSCEFVDELRKMEDHSRMCKYRSAACPYSNIG 132
[Close]
BLAST of SMED30028360 vs. Planmine SMEST
Match: SMESG000025583.1 (SMESG000025583.1)

HSP 1 Score: 127.872 bits (320), Expect = 6.127e-49
Identity = 69/77 (89.61%), Postives = 71/77 (92.21%), Query Frame = 3 
Query:   42 NYESVTEIQHDMXXXXXXXXXXXARCVPCGCRFCFSCISGFLDGGNKICPGFTEECKLEILNLDKNIYVDQPMNIKI 272
            NYESVTEIQHDMLCLLCK LL+NA C  CGCRFCFSCISGFLDG NKICPGFTEECKLEI NLD+NIYVDQPMNIKI
Sbjct:    3 NYESVTEIQHDMLCLLCKKLLVNAHCAQCGCRFCFSCISGFLDGENKICPGFTEECKLEIFNLDENIYVDQPMNIKI 79

HSP 2 Score: 82.8037 bits (203), Expect = 6.127e-49
Identity = 38/42 (90.48%), Postives = 39/42 (92.86%), Query Frame = 1 
Query:  262 ISRLKVKCSEKSCEFVDELRKMEEHSRTCKYRSVACPYSNIG 387
            ISRLKVKC EKSCEFVDELRKME+HSR CKYRS ACPYSNIG
Sbjct:   79 ISRLKVKCHEKSCEFVDELRKMEDHSRMCKYRSAACPYSNIG 120
[Close]
BLAST of SMED30028360 vs. Planmine SMEST
Match: SMESG000025998.1 (SMESG000025998.1)

HSP 1 Score: 124.79 bits (312), Expect = 2.572e-47
Identity = 70/90 (77.78%), Postives = 78/90 (86.67%), Query Frame = 3 
Query:    3 SKLENVGQIMRDYNYESVTEIQHDMXXXXXXXXXXXARCVPCGCRFCFSCISGFLDGGNKICPGFTEECKLEILNLDKNIYVDQPMNIKI 272
            SKLENVGQIMRDYNYESVTEIQ+DMLCLLCK LL+NARC PC CRFCF+CIS FLD   K CPG TEECK E+L+L++NI VD PMN+KI
Sbjct:    4 SKLENVGQIMRDYNYESVTEIQNDMLCLLCKKLLVNARCAPCSCRFCFTCISDFLDHKKKNCPGNTEECKQEMLHLNENICVDYPMNMKI 93

HSP 2 Score: 80.8777 bits (198), Expect = 2.572e-47
Identity = 35/46 (76.09%), Postives = 43/46 (93.48%), Query Frame = 1 
Query:  250 INQ*ISRLKVKCSEKSCEFVDELRKMEEHSRTCKYRSVACPYSNIG 387
            +N  I++L+VKC EKSC++VDELRKME+HS+TCKYRSVACPYSNIG
Sbjct:   89 MNMKIAKLQVKCKEKSCDYVDELRKMEDHSQTCKYRSVACPYSNIG 134
[Close]
BLAST of SMED30028360 vs. Planmine SMEST
Match: SMESG000025980.1 (SMESG000025980.1)

HSP 1 Score: 124.79 bits (312), Expect = 4.366e-47
Identity = 70/90 (77.78%), Postives = 78/90 (86.67%), Query Frame = 3 
Query:    3 SKLENVGQIMRDYNYESVTEIQHDMXXXXXXXXXXXARCVPCGCRFCFSCISGFLDGGNKICPGFTEECKLEILNLDKNIYVDQPMNIKI 272
            SKLENVGQIMRDYNYESVTEIQ+DMLCLLCK LL+NARC PC CRFCF+CIS FLD   K CPG TEECK E+L+L++NI VD PMN+KI
Sbjct:    4 SKLENVGQIMRDYNYESVTEIQNDMLCLLCKKLLVNARCAPCSCRFCFTCISDFLDHKKKNCPGNTEECKQEMLHLNENICVDYPMNMKI 93

HSP 2 Score: 80.1073 bits (196), Expect = 4.366e-47
Identity = 35/46 (76.09%), Postives = 42/46 (91.30%), Query Frame = 1 
Query:  250 INQ*ISRLKVKCSEKSCEFVDELRKMEEHSRTCKYRSVACPYSNIG 387
            +N  I++L+VKC E SC++VDELRKME+HSRTCKYRSVACPYSNIG
Sbjct:   89 MNMKIAKLQVKCKEMSCDYVDELRKMEDHSRTCKYRSVACPYSNIG 134
The following BLAST results are available for this feature:
BLAST of SMED30028360 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028360 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028360 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028360 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028360 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028360 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028360 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028360 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 3
Match NameE-valueIdentityDescription
A0A0F7GWW34.060e-1778.26TNF receptor associated factor 4 (Fragment) OS=Sch... [more]
I1ZID89.567e-1143.68TNF receptor associated factor-2 (Fragment) OS=Sch... [more]
T1E1012.716e-936.26TRAF-6 OS=Dendrocoelum lacteum OX=27895 PE=2 SV=1[more]
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BLAST of SMED30028360 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028360 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028360 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028360 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028360 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028360 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000025558.11.255e-5594.44SMESG000025558.1[more]
SMESG000025583.13.764e-5285.56SMESG000025583.1[more]
SMESG000025583.16.127e-4989.61SMESG000025583.1[more]
SMESG000025998.12.572e-4777.78SMESG000025998.1[more]
SMESG000025980.14.366e-4777.78SMESG000025980.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30028360 ID=SMED30028360|Name=SMED30028360|organism=Schmidtea mediterranea sexual|type=transcript|length=389bp
GTTCAAAATTAGAAAATGTTGGGCAGATTATGAGAGATTATAATTATGAA
AGTGTAACAGAAATCCAGCATGATATGCTATGCTTATTGTGTAAAAATTT
ATTATTAAATGCTCGCTGTGTCCCATGCGGCTGCAGGTTTTGTTTCTCTT
GTATATCAGGCTTTCTAGATGGTGGGAATAAAATATGTCCTGGTTTCACT
GAGGAATGTAAATTGGAAATTCTCAATTTGGATAAAAATATTTATGTGGA
TCAACCAATGAATATCAAGATTAAAAGTGAAATGTTCGGAAAAATCTTGC
GAATTTGTTGATGAATTAAGAAAAATGGAAGAACATTCACGGACGTGCAA
ATATCGGTCAGTAGCATGTCCATATTCCAATATCGGATG
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protein sequence of SMED30028360-orf-1

>SMED30028360-orf-1 ID=SMED30028360-orf-1|Name=SMED30028360-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=103bp
SKLENVGQIMRDYNYESVTEIQHDMLCLLCKNLLLNARCVPCGCRFCFSC
ISGFLDGGNKICPGFTEECKLEILNLDKNIYVDQPMNIKIKSEMFGKILR
IC*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: INTERPRO
TermDefinition
IPR013323SIAH-type
IPR008974TRAF-like
IPR013083Znf_RING/FYVE/PHD
IPR001841Znf_RING
Vocabulary: molecular function
TermDefinition
GO:0008270zinc ion binding
GO:0005515protein binding
GO:0004842ubiquitin-protein transferase activity
GO:0046872metal ion binding
Vocabulary: biological process
TermDefinition
GO:0016567protein ubiquitination