SMED30028348

Overview
NameSMED30028348
Smed IDSMED30028348
Length (bp)3535
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30028348 (SMED30028348) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30028348 (SMED30028348) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30028348 (SMED30028348) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30028348 (SMED30028348) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30028348

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30028348 SmedASXL_017146SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30028348 SmedASXL_017146SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
Smed sexual biotypeSMED30028348SMESG000043908.1 SMESG000028508.1 SMESG000024555.1 SMESG000012010.1 SMESG000081278.1 SMESG000081276.1 SMESG000080770.1 SMESG000065333.1 SMESG000059844.1 SMESG000058646.1 SMESG000055266.1 SMESG000052813.1 SMESG000043147.1 SMESG000037745.1 SMESG000037601.1 SMESG000036070.1 SMESG000034368.1 SMESG000031415.1 SMESG000019663.1 SMESG000010897.1 SMESG000009005.1 SMESG000005029.1 SMESG000077800.1 SMESG000054046.1 SMESG000052058.1 Contig18859newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30028348SMESG000043908.1 SMESG000028508.1 SMESG000024555.1 SMESG000012010.1 SMESG000081278.1 SMESG000081276.1 SMESG000080770.1 SMESG000065333.1 SMESG000059844.1 SMESG000058646.1 SMESG000055266.1 SMESG000052813.1 SMESG000043147.1 SMESG000037745.1 SMESG000037601.1 SMESG000036070.1 SMESG000034368.1 SMESG000031415.1 SMESG000019663.1 SMESG000010897.1 SMESG000009005.1 SMESG000005029.1 SMESG000077800.1 SMESG000054046.1 SMESG000052058.1 Contig18859uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
head regionSMED30028348 dd_Smed_v6_26745_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30028348 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.000467:53208..56746 +17616
Homology
[Close]
BLAST of SMED30028348 vs. UniProt/SwissProt
Match: sp|P34257|TC3A_CAEEL (Transposable element Tc3 transposase OS=Caenorhabditis elegans OX=6239 GN=tc3a PE=1 SV=1)

HSP 1 Score: 83.1889 bits (204), Expect = 5.345e-19
Identity = 49/119 (41.18%), Postives = 72/119 (60.50%), Query Frame = -2 
Query: 1144 YWRDLRKELLIFSKRNFGG-SIMVWQGCSFYDVLPLEFPSS*MNS*EYQQVLQKHLIPFHRNSRHILYFFHQDNASFHFGNITMEWFRDKYPVNVVT--CCSLNCNPI-NTFGVFYATV 1488
            YWRDLRKE ++FS+RNFGG ++MVW   +    L ++F SS MNS +YQ VL+  L  + R+     + F QDNA+ H  N T ++F+ K  +N++     S + NPI N +G+    V
Sbjct:  159 YWRDLRKEPMVFSRRNFGGGTVMVWGAFTEKKKLEIQFVSSKMNSTDYQNVLELELSKYLRHYSRKDFRFQQDNATIHVSNSTRDYFKLK-KINLLDWPARSPDLNPIENLWGILVRIV 276

HSP 2 Score: 35.4242 bits (80), Expect = 5.345e-19
Identity = 13/20 (65.00%), Postives = 17/20 (85.00%), Query Frame = -3 
Query: 1488 QVIYSD*KTFNLDGPDGCNH 1547
            +V++SD K FNLDGPDGC +
Sbjct:  139 KVVFSDEKKFNLDGPDGCRY 158
[Close]
BLAST of SMED30028348 vs. TrEMBL
Match: A0A5J4N2W6 (Uncharacterized protein OS=Paragonimus westermani OX=34504 GN=DEA37_0013878 PE=4 SV=1)

HSP 1 Score: 93.5893 bits (231), Expect = 4.313e-24
Identity = 54/119 (45.38%), Postives = 75/119 (63.03%), Query Frame = -2 
Query: 1144 YWRDLRKELLIFSKRNFGG-SIMVWQGCSFYDVLPLEFPSS*MNS*EYQQVLQKHLIPFHRNSRHILYFFHQDNASFHFGNITMEWFRDKYPVNVVT--CCSLNCNPI-NTFGVFYATV 1488
            YWRDLRKE + FSKRNFGG ++MVW   S   VL L FP++ MNS EY ++L+ +L+PF  ++    + + QDNAS H    T EW R  + +NV+    CS + NPI N +G+   +V
Sbjct:  168 YWRDLRKEPIFFSKRNFGGGTLMVWGAFSSNGVLQLAFPTTRMNSAEYIRILENNLLPFFDHNNPTDWTYQQDNASVHSSRQTREWLR-FHEINVLQWPACSPDQNPIENIWGIIVRSV 285

HSP 2 Score: 37.3502 bits (85), Expect = 4.313e-24
Identity = 26/62 (41.94%), Postives = 34/62 (54.84%), Query Frame = -3 
Query:  993 LLGYSMRRFKANNR*FDSVPQQKPVILTAWNEINQNT---LANSMSERIFVIKEVPLTIKNK 1169
            + G  +R   A NR FDS+ + K  I+ AWNEI  +T   L +SM  RIF +      IKNK
Sbjct:  277 IWGIIVRSVYAQNRHFDSIQELKTEIIKAWNEITPSTRKKLIDSMPNRIFEL------IKNK 332

HSP 3 Score: 32.7278 bits (73), Expect = 4.313e-24
Identity = 13/16 (81.25%), Postives = 14/16 (87.50%), Query Frame = -3 
Query: 1497 VIYSD*KTFNLDGPDG 1544
            VI+SD K FNLDGPDG
Sbjct:  149 VIFSDEKKFNLDGPDG 164
[Close]
BLAST of SMED30028348 vs. TrEMBL
Match: A0A0C2FAW3 (DDE_3 domain-containing protein OS=Ancylostoma duodenale OX=51022 GN=ANCDUO_26058 PE=4 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 5.705e-23
Identity = 50/118 (42.37%), Postives = 69/118 (58.47%), Query Frame = -2 
Query: 1144 YWRDLRKELLIFSKRNFGG-SIMVWQGCSFYDVLPLEFPSS*MNS*EYQQVLQKHLIPFHRNSRHILYFFHQDNASFHFGNITMEWFRD-KYPVNVVTCCSLNCNPI-NTFGVFYATV 1488
            YWRDLRKE ++FS+RNFGG S+M W        L L F S+ M+S EYQ+VL+  L+PF R +R     F QDNA+ H  + T  W ++ +        CS +CNP+ N +G+    V
Sbjct:   27 YWRDLRKEPIMFSRRNFGGGSLMTWAAFGSSGKLELAFVSNRMDSSEYQEVLRTRLLPFLRGARRRSLVFQQDNAAVHVSHSTRSWLQEHRIQAMDWPACSPDCNPVENMWGIIVRQV 144

HSP 2 Score: 33.4982 bits (75), Expect = 5.705e-23
Identity = 14/20 (70.00%), Postives = 16/20 (80.00%), Query Frame = -3 
Query: 1488 QVIYSD*KTFNLDGPDGCNH 1547
            +VI+SD K FNLDGPDG  H
Sbjct:    7 KVIFSDEKKFNLDGPDGYRH 26

HSP 3 Score: 33.113 bits (74), Expect = 5.705e-23
Identity = 19/52 (36.54%), Postives = 30/52 (57.69%), Query Frame = -3 
Query: 1023 LLGYSMRRFKANNR*FDSVPQQKPVILTAWNEINQNTLAN---SMSERIFVI 1169
            + G  +R+   NN+ +++V   K  IL AW++I+  T+A    SM  RIF I
Sbjct:  136 MWGIIVRQVYRNNKQYNTVDSLKTAILEAWDQIDDATVAKLVGSMPNRIFEI 187
[Close]
BLAST of SMED30028348 vs. TrEMBL
Match: A0A368FQJ8 (DDE_3 domain-containing protein OS=Ancylostoma caninum OX=29170 GN=ANCCAN_19813 PE=4 SV=1)

HSP 1 Score: 93.5893 bits (231), Expect = 2.184e-21
Identity = 50/118 (42.37%), Postives = 69/118 (58.47%), Query Frame = -2 
Query: 1144 YWRDLRKELLIFSKRNFGG-SIMVWQGCSFYDVLPLEFPSS*MNS*EYQQVLQKHLIPFHRNSRHILYFFHQDNASFHFGNITMEWFRD-KYPVNVVTCCSLNCNPI-NTFGVFYATV 1488
            YWRDLRKE ++FS+RNFGG S+M W        L L F S+ M+S EYQ+VL+  L+PF R +R     F QDNA+ H  + T  W ++ +        CS +CNP+ N +G+    V
Sbjct:   29 YWRDLRKEPIMFSRRNFGGGSLMTWAAFGSSGKLELAFVSNRMDSSEYQEVLRTRLLPFLRGARRRSLVFQQDNAAVHVSHSTRSWLQEHRIQAMDWPACSPDCNPVENMWGIIVRQV 146

HSP 2 Score: 33.113 bits (74), Expect = 2.184e-21
Identity = 19/52 (36.54%), Postives = 30/52 (57.69%), Query Frame = -3 
Query: 1023 LLGYSMRRFKANNR*FDSVPQQKPVILTAWNEINQNTLA---NSMSERIFVI 1169
            + G  +R+   NN+ +++V   K  IL AW++I+  T+A    SM  RIF I
Sbjct:  138 MWGIIVRQVYRNNKQYNTVESLKTAILEAWDQIDDATVAKLVGSMPNRIFEI 189

HSP 3 Score: 28.1054 bits (61), Expect = 2.184e-21
Identity = 10/13 (76.92%), Postives = 10/13 (76.92%), Query Frame = -3 
Query: 1488 KTFNLDGPDGCNH 1526
            K FNLDGPDG  H
Sbjct:   16 KKFNLDGPDGYRH 28
[Close]
BLAST of SMED30028348 vs. TrEMBL
Match: A0A368H4C5 (Transposase OS=Ancylostoma caninum OX=29170 GN=ANCCAN_02412 PE=4 SV=1)

HSP 1 Score: 97.4413 bits (241), Expect = 3.877e-21
Identity = 72/233 (30.90%), Postives = 110/233 (47.21%), Query Frame = -2 
Query: 1144 AKIVKKLFSDPTVEMKKHRSQENIFSTDEQYIRGAASNSFKCSDIQFEKKLLIIRAHHKDGRFLFARDKMKCD*TKLLNKLHNA*VYLALNTFISGYLF*LKNI*S*RS*WL*PYWRDLRKELLIFSKRNFGG-SIMVWQGCSFYDVLPLEFPSS*MNS*EYQQVLQKHLIPFHRNSRHILYFFHQDNASFHFGNITMEWFRDKYPVNVVT--CCSLNCNPI-NTFGVFYATV 1830
            ++ + +L S+ T+ + + R+Q  +  +     R    N+    +I   +K   +   HK  R LFAR  M     K++              F     F L      R      YWRDLRKE ++FS+RNFGG S+M W        L L F S+ M+S EYQ+VL+ HL+PF R +R     F QDNA+ H  + T  W ++ + +  +    CS +CN + N +G+    V
Sbjct:   67 SRRIGRLASNSTLSIAQMRAQLGLNVSKMTIWRSVRRNTNITREIM--RKAPRLTPQHKKARLLFARSNMTTQWDKVI--------------FSDEKKFNLDGPDGYRH-----YWRDLRKEPIMFSRRNFGGGSLMTWAAFGSSGKLELAFVSNRMDSSEYQEVLRTHLLPFLRGARRRSLVFQQDNAAVHVSHSTRSWLQE-HRIQAMDWPACSPDCNVVENMWGIIVRQV 277

HSP 2 Score: 36.1946 bits (82), Expect = 3.877e-21
Identity = 23/63 (36.51%), Postives = 34/63 (53.97%), Query Frame = -3 
Query:  990 LLGYSMRRFKANNR*FDSVPQQKPVILTAWNEINQNTLA---NSMSERIFVIKEVPLTIKNKC 1169
            + G  +R+   NN+ +++V   K  IL AW++I+  TLA    SM  RIF I      I+N C
Sbjct:  269 MWGIIVRQVYRNNKQYNTVESLKTAILEAWDQIDDATLAKLVGSMPNRIFEI------IRNNC 325
[Close]
BLAST of SMED30028348 vs. TrEMBL
Match: A0A0B1SSQ3 (DDE_3 domain-containing protein OS=Oesophagostomum dentatum OX=61180 GN=OESDEN_12227 PE=4 SV=1)

HSP 1 Score: 82.8037 bits (203), Expect = 2.050e-20
Identity = 48/108 (44.44%), Postives = 62/108 (57.41%), Query Frame = -2 
Query: 1174 YWRDLRKELLIFSKRNFGG-SIMVWQGCSFYDVLPLEFPSS*MNS*EYQQVLQKHLIPFHRNSRHILYFFHQDNASFHFGNITMEWFRDKYPVNVVT--CCSLNCNPI 1488
            YWRDL K  + FS+RNFGG S+MVW   S    L L F S+ MNS EYQ VL+ HL+PF    R   + F ++NA+ H  + T  W   +Y  N +    CS + NPI
Sbjct:   49 YWRDLCKSPVQFSRRNFGGGSVMVWGAFSSECTLSLAFVSNRMNSEEYQNVLENHLLPFLEERRTEAFVFQENNATIHVSSATKSWLW-RYNTNTLDWPACSPDLNPI 155

HSP 2 Score: 35.8094 bits (81), Expect = 2.050e-20
Identity = 22/52 (42.31%), Postives = 29/52 (55.77%), Query Frame = -3 
Query: 1023 LLGYSMRRFKANNR*FDSVPQQKPVILTAWNEINQNTLAN---SMSERIFVI 1169
            L G  +RR  ANNR F++V + K  I  AW EI+   L N   SM  R+F +
Sbjct:  158 LCGIIVRRIYANNRQFNTVNELKDAISRAWMEIHVTVLQNLIGSMRRRLFEV 209

HSP 3 Score: 32.7278 bits (73), Expect = 2.050e-20
Identity = 12/17 (70.59%), Postives = 15/17 (88.24%), Query Frame = -3 
Query: 1497 QVIYSD*KTFNLDGPDG 1547
            +V++SD K FNLDGPDG
Sbjct:   29 KVVFSDEKKFNLDGPDG 45
[Close]
BLAST of SMED30028348 vs. Planmine SMEST
Match: SMESG000028712.1 (SMESG000028712.1)

HSP 1 Score: 77.0258 bits (188), Expect = 2.265e-15
Identity = 41/71 (57.75%), Postives = 48/71 (67.61%), Query Frame = 2 
Query: 2168 ERSVE*LAKWIESEEEKH*RAVSVLFVERPTEGRNPKFTTSFGDSNRLTKFADRMPKTEVRSARQRELNCI 2380
            ER V+ L KWIES+EEKH +A SVLF ERP +  N K TTSFG SNRLT FA R PKTE  +     + C+
Sbjct:   73 ERYVKGLPKWIESQEEKHRKAFSVLFGERPEKDSNLKVTTSFGYSNRLTNFACRRPKTEDNNKILEAIKCL 143
[Close]
BLAST of SMED30028348 vs. Planmine SMEST
Match: SMESG000059177.1 (SMESG000059177.1)

HSP 1 Score: 78.9518 bits (193), Expect = 4.171e-15
Identity = 42/61 (68.85%), Postives = 44/61 (72.13%), Query Frame = 3 
Query: 2163 VGKGLSNDWLNGLKVKKKNIEEQLVCYSLKDRQKAEIQSLLQASEIQIGSQNLRIECRKQR 2345
            V  GLS DWLNG K KK NIEEQLVCYS KDRQK  IQSL Q S+IQI S   R E +KQR
Sbjct:  166 VETGLSKDWLNGSKAKKNNIEEQLVCYSEKDRQKVGIQSLQQTSKIQIDSPTSRTEGQKQR 226
[Close]
BLAST of SMED30028348 vs. Planmine SMEST
Match: SMESG000007018.1 (SMESG000007018.1)

HSP 1 Score: 79.7221 bits (195), Expect = 4.413e-15
Identity = 65/111 (58.56%), Postives = 73/111 (65.77%), Query Frame = 2 
Query: 3233 KLQEVIYSNS*QLELKKLIAKGFTNLCKEFS-----DYRVTRNEDISDEIVEEASEEP*VGLENVHKLSCVALNKDELKNSKDKELGESLEII-----KSKPVEKESQFVE 3535
            KLQEVI   S +LELKK  A+  + LCKEFS     D  V RN DI DEIV+E S+E  VGLEN  KL C AL K EL+NSK KEL ESLEI+     KSK  EKE +FVE
Sbjct:   33 KLQEVIDCKSKELELKKEEAERLSKLCKEFSESILPDSTVIRNVDIVDEIVDEVSKERKVGLENGTKLICDALEKGELENSKGKELVESLEIMKSLIPKSKAGEKERRFVE 143

HSP 2 Score: 77.0258 bits (188), Expect = 2.997e-14
Identity = 53/92 (57.61%), Postives = 60/92 (65.22%), Query Frame = 2 
Query: 3290 AKGFTNLCKEFS-----DYRVTRNEDISDEIVEEASEEP*VGLENVHKLSCVALNKDELKNSKDKELGESLEII-----KSKPVEKESQFVE 3535
            A+  + LCKEFS     D  V RN DI DEIV+E S+E  VGLEN  KL C AL K EL+NSK KEL ESLEI+     KSK  EKE +FVE
Sbjct:  260 AERLSKLCKEFSESILPDSTVIRNVDIVDEIVDEVSKERKVGLENGTKLICDALEKGELENSKGKELVESLEIMKSLIPKSKAGEKERRFVE 351
[Close]
BLAST of SMED30028348 vs. Planmine SMEST
Match: SMESG000007018.1 (SMESG000007018.1)

HSP 1 Score: 79.7221 bits (195), Expect = 8.362e-15
Identity = 65/111 (58.56%), Postives = 73/111 (65.77%), Query Frame = 2 
Query: 3233 KLQEVIYSNS*QLELKKLIAKGFTNLCKEFS-----DYRVTRNEDISDEIVEEASEEP*VGLENVHKLSCVALNKDELKNSKDKELGESLEII-----KSKPVEKESQFVE 3535
            KLQEVI   S +LELKK  A+  + LCKEFS     D  V RN DI DEIV+E S+E  VGLEN  KL C AL K EL+NSK KEL ESLEI+     KSK  EKE +FVE
Sbjct:   33 KLQEVIDCKSKELELKKEEAERLSKLCKEFSESILPDSTVIRNVDIVDEIVDEVSKERKVGLENGTKLICDALEKGELENSKGKELVESLEIMKSLIPKSKAGEKERRFVE 143

HSP 2 Score: 77.0258 bits (188), Expect = 5.387e-14
Identity = 53/92 (57.61%), Postives = 60/92 (65.22%), Query Frame = 2 
Query: 3290 AKGFTNLCKEFS-----DYRVTRNEDISDEIVEEASEEP*VGLENVHKLSCVALNKDELKNSKDKELGESLEII-----KSKPVEKESQFVE 3535
            A+  + LCKEFS     D  V RN DI DEIV+E S+E  VGLEN  KL C AL K EL+NSK KEL ESLEI+     KSK  EKE +FVE
Sbjct:  260 AERLSKLCKEFSESILPDSTVIRNVDIVDEIVDEVSKERKVGLENGTKLICDALEKGELENSKGKELVESLEIMKSLIPKSKAGEKERRFVE 351
[Close]
BLAST of SMED30028348 vs. Planmine SMEST
Match: SMESG000007017.1 (SMESG000007017.1)

HSP 1 Score: 78.9518 bits (193), Expect = 1.982e-14
Identity = 65/111 (58.56%), Postives = 73/111 (65.77%), Query Frame = 2 
Query: 3233 KLQEVIYSNS*QLELKKLIAKGFTNLCKEFS-----DYRVTRNEDISDEIVEEASEEP*VGLENVHKLSCVALNKDELKNSKDKELGESLEII-----KSKPVEKESQFVE 3535
            KLQEVI   S +LELKK  A+  + LCKEFS     D  V RN DI DEIV+E S+E  VGLEN  KL C AL K EL+NSK KEL ESLEI+     KSK  EKE +FVE
Sbjct:  262 KLQEVIDCKSKELELKKEEAERLSKLCKEFSESILPDSTVIRNVDIVDEIVDEVSKERKVGLENGTKLICDALEKGELENSKGKELVESLEIMKSLIPKSKAGEKERRFVE 372

HSP 2 Score: 78.9518 bits (193), Expect = 1.982e-14
Identity = 65/111 (58.56%), Postives = 73/111 (65.77%), Query Frame = 2 
Query: 3233 KLQEVIYSNS*QLELKKLIAKGFTNLCKEFS-----DYRVTRNEDISDEIVEEASEEP*VGLENVHKLSCVALNKDELKNSKDKELGESLEII-----KSKPVEKESQFVE 3535
            KLQEVI   S +LELKK  A+  + LCKEFS     D  V RN DI DEIV+E S+E  VGLEN  KL C AL K EL+NSK KEL ESLEI+     KSK  EKE +FVE
Sbjct:  592 KLQEVIDCKSKELELKKEEAERLSKLCKEFSESILPDSTVIRNVDIVDEIVDEVSKERKVGLENGTKLICDALEKGELENSKGKELVESLEIMKSLIPKSKAGEKERRFVE 702

HSP 3 Score: 78.9518 bits (193), Expect = 1.982e-14
Identity = 65/111 (58.56%), Postives = 73/111 (65.77%), Query Frame = 2 
Query: 3233 KLQEVIYSNS*QLELKKLIAKGFTNLCKEFS-----DYRVTRNEDISDEIVEEASEEP*VGLENVHKLSCVALNKDELKNSKDKELGESLEII-----KSKPVEKESQFVE 3535
            KLQEVI   S +LELKK  A+  + LCKEFS     D  V RN DI DEIV+E S+E  VGLEN  KL C AL K EL+NSK KEL ESLEI+     KSK  EKE +FVE
Sbjct:  922 KLQEVIDCKSKELELKKEEAERLSKLCKEFSESILPDSTVIRNVDIVDEIVDEVSKERKVGLENGTKLICDALEKGELENSKGKELVESLEIMKSLIPKSKAGEKERRFVE 1032

HSP 4 Score: 78.9518 bits (193), Expect = 1.982e-14
Identity = 65/111 (58.56%), Postives = 73/111 (65.77%), Query Frame = 2 
Query: 3233 KLQEVIYSNS*QLELKKLIAKGFTNLCKEFS-----DYRVTRNEDISDEIVEEASEEP*VGLENVHKLSCVALNKDELKNSKDKELGESLEII-----KSKPVEKESQFVE 3535
            KLQEVI   S +LELKK  A+  + LCKEFS     D  V RN DI DEIV+E S+E  VGLEN  KL C AL K EL+NSK KEL ESLEI+     KSK  EKE +FVE
Sbjct: 1252 KLQEVIDCKSKELELKKEEAERLSKLCKEFSESILPDSTVIRNVDIVDEIVDEVSKERKVGLENGTKLICDALEKGELENSKGKELVESLEIMKSLIPKSKAGEKERRFVE 1362
The following BLAST results are available for this feature:
BLAST of SMED30028348 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028348 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028348 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028348 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028348 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028348 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028348 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 1
Match NameE-valueIdentityDescription
sp|P34257|TC3A_CAEEL5.345e-1941.18Transposable element Tc3 transposase OS=Caenorhabd... [more]
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BLAST of SMED30028348 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A5J4N2W64.313e-2445.38Uncharacterized protein OS=Paragonimus westermani ... [more]
A0A0C2FAW35.705e-2342.37DDE_3 domain-containing protein OS=Ancylostoma duo... [more]
A0A368FQJ82.184e-2142.37DDE_3 domain-containing protein OS=Ancylostoma can... [more]
A0A368H4C53.877e-2130.90Transposase OS=Ancylostoma caninum OX=29170 GN=ANC... [more]
A0A0B1SSQ32.050e-2044.44DDE_3 domain-containing protein OS=Oesophagostomum... [more]
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BLAST of SMED30028348 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028348 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028348 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028348 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028348 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028348 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000028712.12.265e-1557.75SMESG000028712.1[more]
SMESG000059177.14.171e-1568.85SMESG000059177.1[more]
SMESG000007018.14.413e-1558.56SMESG000007018.1[more]
SMESG000007018.18.362e-1558.56SMESG000007018.1[more]
SMESG000007017.11.982e-1458.56SMESG000007017.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30028348 ID=SMED30028348|Name=SMED30028348|organism=Schmidtea mediterranea sexual|type=transcript|length=3535bp
ATTTTACAGGTAAATAATTATTTTAAAACCATTTGATTAAAAATATTTAA
ATTAGTTTATCGAAATTTTAATTTTTAACCAGTTGGATAATTTTTTTGTC
ATTTAAAACAATTACTTAATTAATTTTTTTAAAATGATTTTTGTGAAAGG
CAAATAAAATTACAAGTTTTTGATGTGCATCTGATTTTATTTTTCAAATG
TATCTTTTCTGTGGTTTCGTCACAAATTCACGGGATAAGATTCCGGGCAA
CCTCGAGTTGGTATGGATTAAAAGTTAAATAGGCTCAGAACTTGGCTACC
ATTAAAAATCGCAAAATGTTACATTAAATTTTTAATCTGAATAACTAGAT
TTCTAATTGTTTCGTAGCTTTAAACAATTTTTATTTAATTTTGACTAAAT
CCGAATTTTGAATAAATCAAAACTCCTAGATCACTAATTTAATCCGATTT
AAACCTTTGCACGATTCAAAATGAAATCTAAAATTATGAAGCACGGAACT
TAAGTTTCATTGTAAAAGTCGTGACAAAATCACATTGTTACAACATTAGT
GAGTTCAGGATGTGACGACCAAAAAGAAACTTGGATTATTTTTTATACTT
AAGGCCTTATTATTGGTATGAAAGTTTTATAGTTTTAAAATCTTGTGGTA
GAATTAAAGACTAAACCATAAATTATTTTGAACAAAAAATTTTAACTTAA
CAATATTTTCTGGACCGACTTGATAAAAATATATGGGGCACCAAATAGTT
TTATTTTTCGTCAATTAAATTAAATTTAAGAATTTTCCATTTACAGTGCA
TATCATAATTATAGCCCCCTTTCATTTTTTCTGATTCTACTCCTTAACGA
AATGGGGGTTTTCCACTCATACGAGGATTTTATTGAGCTATTATTGGAAG
AAAATTACTGAAAAAATGATTTTTCCGTCGTTTCATAATTATTGCCCTTC
TTTAAATAATGTGATTGACAAATGAAACTAAGACAAAATACATTTATTTT
TAATAGTCAGTGGAACCTCTTTTATTACGAATATTCTTTCAGACATGCTA
TTGGCGAGTGTATTTTGATTAATTTCATTCCAGGCTGTCAAAATTACTGG
TTTCTGTTGGGGAACACTGTCAAACTAACGGTTGTTCGCTTTAAACCGTC
GCATAGAATACCCCAAAAGTGTTGATTGGGTTACAATTAAGAGAGCAGCA
GGTCACTACATTAACGGGATATTTGTCTCTAAACCACTCCATGGTTATGT
TGCCAAAATGAAAGCTAGCATTATCTTGATGAAAAAAGTAAAGAATATGG
CGTGAATTCCGATGGAAGGGAATAAGATGTTTCTGGAGAACCTGTTGATA
CTCTTAACTGTTCATTCAGCTGGATGGAAACTCCAGAGGAAGTACATCAT
AGAAAGAGCAACCTTGCCAAACCATGATACTTCCTCCAAAGTTTCGCTTG
GAAAATATTAATAGTTCTTTACGAAGATCTCTCCAGTATGGTTACAACCA
TCAGGACCGTCAAGATTAAATGTTTTTTAGTCAGAATAAATAACCTGAAA
TGAAAGTATTTAATGCTAAATACACTTAAGCATTGTGTAGCTTATTAAGT
AGCTTAGTCTAATCACACTTCATTTTATCGCGTGCGAATAAAAATCTACC
ATCTTTATGATGAGCTCTTATTATTAGCAATTTTTTTTCGAACTGAATGT
CTGAACATTTAAAGGAATTAGAAGCAGCCCCACGAATGTATTGTTCGTCA
GTGGAGAAAATATTTTCTTGCGATCGGTGTTTTTTCATTTCGACAGTCGG
GTCTGAAAAAAGTTTTTTTACTATCTTGGCTGATCTACTTGAGCTCGACC
AATAGCTTTGTTGCTTTTTCGCTCATCGGCTAAAGTCACAAATTAGGCCT
TTTCAAACTTAGACAACATTGTATCACTTTGGATATTTACTGAAAAATGA
AAGAAAACTATATGTAAATATATAATAAATCTTTAATAAATGTTGAAACT
ATAATAAATTTCAACAAAGGAGAATATAATTATGAAACGTCTGAAAAATC
TTTTTTTCAATAATTTAAACACAAAAATAAATTATGGTTTTGTTTTGGGT
GTTGTGGCTCAATAAAATCCTCAGATAAGTGGTAGGCCCCCTTTTGTTAA
GAAGTAGAATTGGTCGGGAAAGGTCTGTCGAATGATTGGCTAAATGGATT
GAAAGTGAAGAAGAAAAACATTGAAGAGCAGTTAGTGTGTTATTCGTTGA
AAGACCGACAGAAGGCAGAAATCCAAAGTTTACTACAAGCTTCGGAGATT
CAAATAGGCTCACAAAATTTGCGGATAGAATGCCGAAAACAGAGGTTAGA
AGCGCGCGACAAAGGGAGCTGAATTGTATCAAGCTGAATGTCAAAGAACG
ACATGAATTTGTGACGATTAAATGACTGTTACAATTGTTTTTCGACAGCT
CATATGATTAGGCAATGTGAAAAACCGAGGTTTTTAAGGACAGGAATTCC
ACAATAAAACATAATTTGCTTCATGACTCAGGTAGCAGAATTGATGGGAA
ACAAAAGCCGAGCAAAGAAAATACCTCAATGATTACTTTTCAAGAGACAC
TAAGTCAGAAAATGTATGTAACGTTGCGGGGATGATCACAATAAAATTAA
TCAACGAGATTCGAGTTAATGCATTGCTGGACGACGCAAGCAATGTCAGT
TGCATTAGTGATTAGGCAGCGCAAGAAGTGGGTGTCGGAGGACAATGGTA
AAGCACTCAAGTTGGCGTAATTGGTGGAAAATCGGAAATGCTGGAAACGA
GCCCAGTAATAGTTTCTGTTGACAATTACTAATAATAAAGTAAAAGTAAA
CTTCGAATGTCATAGGAAACCAAGAGATGTGCAATTGGGGAAAATTGTCG
TGGAACTGGAAGCATTTGCGGAATATAGACTTTCCAGTTTTAATTGGTAT
GTTACCCGCACCTACATCGATCTACCCGAGAAATAAAAGGAGAGAACCAG
TGCCGAAGTTGACACTCCTCGGATGGACGTGTCTGGGTAATATTAATAGT
GATAAAGCTCCGAGATGCTCACATTTGGCACAGAGCTATTTGTCAAAAAT
GGAAAAGGTAAATGAAGAGGTACTGCAATGTTTGGAAATTGAGGAGACAC
AAGTTCGCAGAAATGAATCAAATCAAGATAAAAGAAGATTTATGCAAAAA
TCAAATAATACTTTACTGGAATTGATTGTTATAAACTACAAGAGGTGATC
TATAGTAATTCTTAACAATTGGAATTGAAGAAATTAATTGCTAAAGGGTT
TACCAATTTGTGCAAAGAATTCTCAGATTACAGAGTAACTAGGAATGAAG
ATATTTCTGACGAAATCGTTGAAGAAGCAAGTGAAGAACCTTAAGTTGGA
CTTGAAAATGTTCATAAATTATCGTGTGTTGCCTTGAATAAAGATGAATT
GAAGAACTCAAAAGATAAAGAACTTGGTGAAAGTTTAGAAATTATTAAAT
CAAAGCCTGTAGAGAAAGAAAGCCAATTCGTTGAA
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protein sequence of SMED30028348-orf-1

>SMED30028348-orf-1 ID=SMED30028348-orf-1|Name=SMED30028348-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=124bp
MCNWGKLSWNWKHLRNIDFPVLIGMLPAPTSIYPRNKRREPVPKLTLLGW
TCLGNINSDKAPRCSHLAQSYLSKMEKVNEEVLQCLEIEETQVRRNESNQ
DKRRFMQKSNNTLLELIVINYKR*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000418head
PLANA:0002109X1 cell
PLANA:0002111X2 cell
Vocabulary: INTERPRO
TermDefinition
IPR001878Znf_CCHC
Vocabulary: molecular function
TermDefinition
GO:0008270zinc ion binding
GO:0003676nucleic acid binding