Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16

Overview
NamePre-mRNA-splicing factor ATP-dependent RNA helicase PRP16
Smed IDSMED30028341
Length (bp)3124
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 (SMED30028341) t-SNE clustered cells

Violin plots show distribution of expression levels for Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 (SMED30028341) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 (SMED30028341) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 (SMED30028341) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30028341

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30028341h1SMcG0022323 dd_Smed_v4_1945_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30028341h1SMcG0022323 dd_Smed_v4_1945_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30028341h1SMcG0022323 dd_Smed_v4_1945_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30028341h1SMcG0022323 dd_Smed_v4_1945_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. Ensembl Human
Match: DHX38 (DEAH-box helicase 38 [Source:HGNC Symbol;Acc:HGNC:17211])

HSP 1 Score: 500.745 bits (1288), Expect = 0.000e+0
Identity = 296/603 (49.09%), Postives = 404/603 (67.00%), Query Frame = 3
Query:  243 RHKQWEEMTPSTSLRDETGRYSEIRTPMIDRSIK----------TPSV--TSWDEDDVTFKRPDTVTSFRRN----------DKSEIKWSPDKNEEELDREWYLND-GME--YNPFPNFSDEVLEKKKQQLQRQTSIVAKSRKSARAKQFEETNRAWEESKLINSGAVVRKGFNVEIDNEDTGEDRIHLHVHNILPPFLDGRIVFTKQFEPVIPVKDENSDMAKIAKNGSQMVKNYRDIKEKMKLQKRTLDMTGTNFGKLMGLEKKETLDKW----DETNYKDSMKYCDKITDKIALDSE--QKSKIDDQRKRLPIFKVRRDLIRLIRENQVIVIVGETGSGKTTQLTQYLLEENYHHKGIIGCTQPXXXXXXXXXXXXXDEVGTKLGDKVGYSIRFEDCKSENTMIKYMTDGIXXXXXXXXXXXXAYSVIIMDEAHERTLNTDILFGLLREIIQRRSDLHLVVTSATMDAEKFSNFFGKCEIFKIPGRTFPVDVIYEKSSILDPVAAAVNKAVDTHLGADSKDGDILVFMSGQEDVEVTCDKIDETLEKVKDTNPPDLKVEPLYSQLSSERQSEIFKINTSGIRRCIVATNIAETSVTLDGI 1958
            R  QWE  +P+ S RD + R   + T   DRS++          TPS     W +D         ++  R            D  E +   + ++ + DR+WY+ D G +  +NP    S++ + +++Q L +Q     + R SA+ +Q  E N  WE ++++ SG V R    V+ D E+    ++HL VHN++PPFLDGRIVFTKQ EPVIPVKD  SD+A IA+ GSQ V+ +R+ KE+ K Q +  ++ GT  G +MG++K+E  DK      + +Y+   K+ D +  K    SE  +K  I +QR+ LPIF V+++L+ +IR+N ++++VGETGSGKTTQLTQYL E+ Y   G+IGCTQPRRVAA+SVAKRV++E+G  LG++VGY+IRFEDC SENT+IKYMTDGILLRESLR+ DLD YS IIMDEAHER+LNTD+LFGLLRE++ RRSDL L+VTSATMDAEKF+ FFG   IF IPGRTFPVD+++ K+   D V AAV +++  HL      GDIL+FM GQED+EVT D+I E LE+++  N P L V P+YSQL S+ Q++IF+    G+R+CIVATNIAETS+T+DGI
Sbjct:  226 RRSQWESPSPTPSYRD-SERSHRLSTRDRDRSVRGKYSDDTPLPTPSYKYNEWADDRRHLGSTPRLSRGRGRREEGEEGISFDTEEERQQWEDDQRQADRDWYMMDEGYDEFHNPLAYSSEDYVRRREQHLHKQK----QKRISAQRRQINEDNERWETNRMLTSGVVHR--LEVDEDFEEDNAAKVHLMVHNLVPPFLDGRIVFTKQPEPVIPVKDATSDLAIIARKGSQTVRKHREQKERKKAQHKHWELAGTKLGDIMGVKKEEEPDKAVTEDGKVDYRTEQKFADHMKRKSEASSEFAKKKSILEQRQYLPIFAVQQELLTIIRDNSIVIVVGETGSGKTTQLTQYLHEDGYTDYGMIGCTQPRRVAAMSVAKRVSEEMGGNLGEEVGYAIRFEDCTSENTLIKYMTDGILLRESLREADLDHYSAIIMDEAHERSLNTDVLFGLLREVVARRSDLKLIVTSATMDAEKFAAFFGNVPIFHIPGRTFPVDILFSKTPQEDYVEAAVKQSLQVHLSG--APGDILIFMPGQEDIEVTSDQIVEHLEELE--NAPALAVLPIYSQLPSDLQAKIFQKAPDGVRKCIVATNIAETSLTVDGI 817          

HSP 2 Score: 474.167 bits (1219), Expect = 0.000e+0
Identity = 217/322 (67.39%), Postives = 264/322 (81.99%), Query Frame = 1
Query: 1960 GMDSLQIYPISQASANQRAGRAGRTGPGICYRLYTINQYENDMLPMTVPEIQRTNLTNVVLLLKSLNVQDLMKFYFMDSPPQDNILNSMYHLWMLGALDAVGNLTELGRTMSEFPLDPSLSKMLITSVKHGCSEEMLTIVSMLSVPAIFSRPTGHQEESDCAHEKFQVAESDHLTLLNVYRLWRESGYSANFCKKYYIHNKSMKRVREVRQQLKEIMIKLKMSLASSG-DFDMIRYCICSAYFNQAARWKGLGEYVNLRSGVVCHLHPTSSLYNMAFNADYVVYHELLMTSKEFMNYVTFVDARWLAELGHMFYSVKEKGQS 2922
            GMD+LQIYPISQA+ANQR+GRAGRTGPG C+RLYT + Y+N++L  TVPEIQRTNL NVVLLLKSL VQDL++F+FMD PP+DN+LNSMY LW+LGALD  G LT  GR M EFPLDP+LSKMLI S   GCS E+L IVSMLSVPAIF RP G +EESD   EKF V ESDHLT LNVY  W+ + YS  +C  ++IH K+M++VREVR QLK+IM++ +MSLAS G D+D++R CIC+AYF+QAA+ KG+GEYVN+R+G+ CHLHPTSSL+ M +  DY+VYHEL+MT+KE+M  VT VD  WLAELG MFYSVK+ G+S
Sbjct:  836 GMDALQIYPISQANANQRSGRAGRTGPGQCFRLYTQSAYKNELLTTTVPEIQRTNLANVVLLLKSLGVQDLLQFHFMDPPPEDNMLNSMYQLWILGALDNTGGLTSTGRLMVEFPLDPALSKMLIVSCDMGCSSEILLIVSMLSVPAIFYRPKGREEESDQIREKFAVPESDHLTYLNVYLQWKNNNYSTIWCNDHFIHAKAMRKVREVRAQLKDIMVQQRMSLASCGTDWDIVRKCICAAYFHQAAKLKGIGEYVNIRTGMPCHLHPTSSLFGMGYTPDYIVYHELVMTTKEYMQCVTAVDGEWLAELGPMFYSVKQAGKS 1157          
BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. Ensembl Human
Match: DHX16 (DEAH-box helicase 16 [Source:HGNC Symbol;Acc:HGNC:2739])

HSP 1 Score: 290.812 bits (743), Expect = 7.796e-157
Identity = 149/328 (45.43%), Postives = 214/328 (65.24%), Query Frame = 1
Query: 1933 KPLSPSTGYGMDSLQIYPISQASANQRAGRAGRTGPGICYRLYTINQYENDMLPMTVPEIQRTNLTNVVLLLKSLNVQDLMKFYFMDSPPQDNILNSMYHLWMLGALDAVGNLTELGRTMSEFPLDPSLSKMLITSVKHGCSEEMLTIVSMLSV-PAIFSRPTGHQEESDCAHEKFQVAESDHLTLLNVYRLWRESGYSANFCKKYYIHNKSMKRVREVRQQLKEIMIKLKMSLAS-SGDFDMIRYCICSAYFNQAARWKGLGEYVNLRSGVVCHLHPTSSLYNMAFNADYVVYHELLMTSKEFMNYVTFVDARWLAELGHMFYSVKE 2910
            K  +P TG  M+SL + P S+ASANQRAGRAGR   G C+RLYT   Y++++   TVPEIQRT+L NVVLLLKSL + DLM F F+D PP + +L ++  L+ LGAL+ +G LT  GR M+E P+DP LSKM++ S K+ CSEE+LT+ +MLSV  +IF RP      +D A   F +   DHL LLNVY  W ESGYS+ +C + ++  +SM+R R+VR+QL+ ++ ++++ L+S  GD+  +R  I + YF   AR    G Y  ++      +HP SSL+       +++YHEL++T+KEFM  V  +++ WL E+   +Y  KE
Sbjct:  698 KSYNPRTG--MESLTVTPCSKASANQRAGRAGRVAAGKCFRLYTAWAYQHELEETTVPEIQRTSLGNVVLLLKSLGIHDLMHFDFLDPPPYETLLLALEQLYALGALNHLGELTTSGRKMAELPVDPMLSKMILASEKYSCSEEILTVAAMLSVNNSIFYRPKDKVVHADNARVNFFLPGGDHLVLLNVYTQWAESGYSSQWCYENFVQFRSMRRARDVREQLEGLLERVEVGLSSCQGDYIRVRKAITAGYFYHTARLTRSG-YRTVKQQQTVFIHPNSSLFEQ--QPRWLLYHELVLTTKEFMRQVLEIESSWLLEVAPHYYKAKE 1020          

HSP 2 Score: 285.804 bits (730), Expect = 7.796e-157
Identity = 156/304 (51.32%), Postives = 219/304 (72.04%), Query Frame = 3
Query: 1050 SEQKSKIDDQRKRLPIFKVRRDLIRLIRENQVIVIVGETGSGKTTQLTQYLLEENYHHKGI-IGCTQPXXXXXXXXXXXXXDEVGTKLGDKVGYSIRFEDCKSENTMIKYMTDGIXXXXXXXXXXXXAYSVIIMDEAHERTLNTDILFGLLREIIQRRSDLHLVVTSATMDAEKFSNFFGKCEIFKIPGRTFPVDVIYEKSSILDPVAAAVNKAVDTHLGADSKDGDILVFMSGQEDVEVTCDKIDETLEKVKDTNPPDLKVEPLYSQLSSERQSEIFKINTSGIRRCIVATNIAETSVTLDGI 1958
            ++QK  I   R+ LP+F  R +L+  I  +QV++I GETGSGKTTQ+ QYL EE Y +KG+ I CTQPRRVAA+SVA RVA E+G KLG++VGYSIRFEDC SE T+++YMTDG+LLRE L + DL +YSV+++DEAHERTL+TDILFGL++++ + R +L ++V SATMD  +FS FF    +F+IPGR FPVD+ Y K+   D + A V   +  H+      GDILVF++GQE++E  C+ + +   ++  +   +L V P+Y+ L S+ Q+ IF+    G R+ +VATNIAETS+T++GI
Sbjct:  386 AQQKESIQAVRRSLPVFPFREELLAAIANHQVLIIEGETGSGKTTQIPQYLFEEGYTNKGMKIACTQPRRVAAMSVAARVAREMGVKLGNEVGYSIRFEDCTSERTVLRYMTDGMLLREFLSEPDLASYSVVMVDEAHERTLHTDILFGLIKDVARFRPELKVLVASATMDTARFSTFFDGAPVFRIPGRRFPVDIFYTKAPEADYLEACVVSVLQIHV--TQPPGDILVFLTGQEEIEAACEMLQDRCRRL-GSKIRELLVLPIYANLPSDMQARIFQPTPPGARKVVVATNIAETSLTIEGI 686          
BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. Ensembl Human
Match: DHX16 (DEAH-box helicase 16 [Source:HGNC Symbol;Acc:HGNC:2739])

HSP 1 Score: 290.426 bits (742), Expect = 9.362e-157
Identity = 149/328 (45.43%), Postives = 214/328 (65.24%), Query Frame = 1
Query: 1933 KPLSPSTGYGMDSLQIYPISQASANQRAGRAGRTGPGICYRLYTINQYENDMLPMTVPEIQRTNLTNVVLLLKSLNVQDLMKFYFMDSPPQDNILNSMYHLWMLGALDAVGNLTELGRTMSEFPLDPSLSKMLITSVKHGCSEEMLTIVSMLSV-PAIFSRPTGHQEESDCAHEKFQVAESDHLTLLNVYRLWRESGYSANFCKKYYIHNKSMKRVREVRQQLKEIMIKLKMSLAS-SGDFDMIRYCICSAYFNQAARWKGLGEYVNLRSGVVCHLHPTSSLYNMAFNADYVVYHELLMTSKEFMNYVTFVDARWLAELGHMFYSVKE 2910
            K  +P TG  M+SL + P S+ASANQRAGRAGR   G C+RLYT   Y++++   TVPEIQRT+L NVVLLLKSL + DLM F F+D PP + +L ++  L+ LGAL+ +G LT  GR M+E P+DP LSKM++ S K+ CSEE+LT+ +MLSV  +IF RP      +D A   F +   DHL LLNVY  W ESGYS+ +C + ++  +SM+R R+VR+QL+ ++ ++++ L+S  GD+  +R  I + YF   AR    G Y  ++      +HP SSL+       +++YHEL++T+KEFM  V  +++ WL E+   +Y  KE
Sbjct:  698 KSYNPRTG--MESLTVTPCSKASANQRAGRAGRVAAGKCFRLYTAWAYQHELEETTVPEIQRTSLGNVVLLLKSLGIHDLMHFDFLDPPPYETLLLALEQLYALGALNHLGELTTSGRKMAELPVDPMLSKMILASEKYSCSEEILTVAAMLSVNNSIFYRPKDKVVHADNARVNFFLPGGDHLVLLNVYTQWAESGYSSQWCYENFVQFRSMRRARDVREQLEGLLERVEVGLSSCQGDYIRVRKAITAGYFYHTARLTRSG-YRTVKQQQTVFIHPNSSLFEQ--QPRWLLYHELVLTTKEFMRQVLEIESSWLLEVAPHYYKAKE 1020          

HSP 2 Score: 285.804 bits (730), Expect = 9.362e-157
Identity = 156/304 (51.32%), Postives = 219/304 (72.04%), Query Frame = 3
Query: 1050 SEQKSKIDDQRKRLPIFKVRRDLIRLIRENQVIVIVGETGSGKTTQLTQYLLEENYHHKGI-IGCTQPXXXXXXXXXXXXXDEVGTKLGDKVGYSIRFEDCKSENTMIKYMTDGIXXXXXXXXXXXXAYSVIIMDEAHERTLNTDILFGLLREIIQRRSDLHLVVTSATMDAEKFSNFFGKCEIFKIPGRTFPVDVIYEKSSILDPVAAAVNKAVDTHLGADSKDGDILVFMSGQEDVEVTCDKIDETLEKVKDTNPPDLKVEPLYSQLSSERQSEIFKINTSGIRRCIVATNIAETSVTLDGI 1958
            ++QK  I   R+ LP+F  R +L+  I  +QV++I GETGSGKTTQ+ QYL EE Y +KG+ I CTQPRRVAA+SVA RVA E+G KLG++VGYSIRFEDC SE T+++YMTDG+LLRE L + DL +YSV+++DEAHERTL+TDILFGL++++ + R +L ++V SATMD  +FS FF    +F+IPGR FPVD+ Y K+   D + A V   +  H+      GDILVF++GQE++E  C+ + +   ++  +   +L V P+Y+ L S+ Q+ IF+    G R+ +VATNIAETS+T++GI
Sbjct:  386 AQQKESIQAVRRSLPVFPFREELLAAIANHQVLIIEGETGSGKTTQIPQYLFEEGYTNKGMKIACTQPRRVAAMSVAARVAREMGVKLGNEVGYSIRFEDCTSERTVLRYMTDGMLLREFLSEPDLASYSVVMVDEAHERTLHTDILFGLIKDVARFRPELKVLVASATMDTARFSTFFDDAPVFRIPGRRFPVDIFYTKAPEADYLEACVVSVLQIHV--TQPPGDILVFLTGQEEIEAACEMLQDRCRRL-GSKIRELLVLPIYANLPSDMQARIFQPTPPGARKVVVATNIAETSLTIEGI 686          
BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. Ensembl Human
Match: DHX16 (DEAH-box helicase 16 [Source:HGNC Symbol;Acc:HGNC:2739])

HSP 1 Score: 290.426 bits (742), Expect = 9.362e-157
Identity = 149/328 (45.43%), Postives = 214/328 (65.24%), Query Frame = 1
Query: 1933 KPLSPSTGYGMDSLQIYPISQASANQRAGRAGRTGPGICYRLYTINQYENDMLPMTVPEIQRTNLTNVVLLLKSLNVQDLMKFYFMDSPPQDNILNSMYHLWMLGALDAVGNLTELGRTMSEFPLDPSLSKMLITSVKHGCSEEMLTIVSMLSV-PAIFSRPTGHQEESDCAHEKFQVAESDHLTLLNVYRLWRESGYSANFCKKYYIHNKSMKRVREVRQQLKEIMIKLKMSLAS-SGDFDMIRYCICSAYFNQAARWKGLGEYVNLRSGVVCHLHPTSSLYNMAFNADYVVYHELLMTSKEFMNYVTFVDARWLAELGHMFYSVKE 2910
            K  +P TG  M+SL + P S+ASANQRAGRAGR   G C+RLYT   Y++++   TVPEIQRT+L NVVLLLKSL + DLM F F+D PP + +L ++  L+ LGAL+ +G LT  GR M+E P+DP LSKM++ S K+ CSEE+LT+ +MLSV  +IF RP      +D A   F +   DHL LLNVY  W ESGYS+ +C + ++  +SM+R R+VR+QL+ ++ ++++ L+S  GD+  +R  I + YF   AR    G Y  ++      +HP SSL+       +++YHEL++T+KEFM  V  +++ WL E+   +Y  KE
Sbjct:  698 KSYNPRTG--MESLTVTPCSKASANQRAGRAGRVAAGKCFRLYTAWAYQHELEETTVPEIQRTSLGNVVLLLKSLGIHDLMHFDFLDPPPYETLLLALEQLYALGALNHLGELTTSGRKMAELPVDPMLSKMILASEKYSCSEEILTVAAMLSVNNSIFYRPKDKVVHADNARVNFFLPGGDHLVLLNVYTQWAESGYSSQWCYENFVQFRSMRRARDVREQLEGLLERVEVGLSSCQGDYIRVRKAITAGYFYHTARLTRSG-YRTVKQQQTVFIHPNSSLFEQ--QPRWLLYHELVLTTKEFMRQVLEIESSWLLEVAPHYYKAKE 1020          

HSP 2 Score: 285.804 bits (730), Expect = 9.362e-157
Identity = 156/304 (51.32%), Postives = 219/304 (72.04%), Query Frame = 3
Query: 1050 SEQKSKIDDQRKRLPIFKVRRDLIRLIRENQVIVIVGETGSGKTTQLTQYLLEENYHHKGI-IGCTQPXXXXXXXXXXXXXDEVGTKLGDKVGYSIRFEDCKSENTMIKYMTDGIXXXXXXXXXXXXAYSVIIMDEAHERTLNTDILFGLLREIIQRRSDLHLVVTSATMDAEKFSNFFGKCEIFKIPGRTFPVDVIYEKSSILDPVAAAVNKAVDTHLGADSKDGDILVFMSGQEDVEVTCDKIDETLEKVKDTNPPDLKVEPLYSQLSSERQSEIFKINTSGIRRCIVATNIAETSVTLDGI 1958
            ++QK  I   R+ LP+F  R +L+  I  +QV++I GETGSGKTTQ+ QYL EE Y +KG+ I CTQPRRVAA+SVA RVA E+G KLG++VGYSIRFEDC SE T+++YMTDG+LLRE L + DL +YSV+++DEAHERTL+TDILFGL++++ + R +L ++V SATMD  +FS FF    +F+IPGR FPVD+ Y K+   D + A V   +  H+      GDILVF++GQE++E  C+ + +   ++  +   +L V P+Y+ L S+ Q+ IF+    G R+ +VATNIAETS+T++GI
Sbjct:  386 AQQKESIQAVRRSLPVFPFREELLAAIANHQVLIIEGETGSGKTTQIPQYLFEEGYTNKGMKIACTQPRRVAAMSVAARVAREMGVKLGNEVGYSIRFEDCTSERTVLRYMTDGMLLREFLSEPDLASYSVVMVDEAHERTLHTDILFGLIKDVARFRPELKVLVASATMDTARFSTFFDDAPVFRIPGRRFPVDIFYTKAPEADYLEACVVSVLQIHV--TQPPGDILVFLTGQEEIEAACEMLQDRCRRL-GSKIRELLVLPIYANLPSDMQARIFQPTPPGARKVVVATNIAETSLTIEGI 686          
BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. Ensembl Human
Match: DHX16 (DEAH-box helicase 16 [Source:HGNC Symbol;Acc:HGNC:2739])

HSP 1 Score: 290.426 bits (742), Expect = 9.362e-157
Identity = 149/328 (45.43%), Postives = 214/328 (65.24%), Query Frame = 1
Query: 1933 KPLSPSTGYGMDSLQIYPISQASANQRAGRAGRTGPGICYRLYTINQYENDMLPMTVPEIQRTNLTNVVLLLKSLNVQDLMKFYFMDSPPQDNILNSMYHLWMLGALDAVGNLTELGRTMSEFPLDPSLSKMLITSVKHGCSEEMLTIVSMLSV-PAIFSRPTGHQEESDCAHEKFQVAESDHLTLLNVYRLWRESGYSANFCKKYYIHNKSMKRVREVRQQLKEIMIKLKMSLAS-SGDFDMIRYCICSAYFNQAARWKGLGEYVNLRSGVVCHLHPTSSLYNMAFNADYVVYHELLMTSKEFMNYVTFVDARWLAELGHMFYSVKE 2910
            K  +P TG  M+SL + P S+ASANQRAGRAGR   G C+RLYT   Y++++   TVPEIQRT+L NVVLLLKSL + DLM F F+D PP + +L ++  L+ LGAL+ +G LT  GR M+E P+DP LSKM++ S K+ CSEE+LT+ +MLSV  +IF RP      +D A   F +   DHL LLNVY  W ESGYS+ +C + ++  +SM+R R+VR+QL+ ++ ++++ L+S  GD+  +R  I + YF   AR    G Y  ++      +HP SSL+       +++YHEL++T+KEFM  V  +++ WL E+   +Y  KE
Sbjct:  698 KSYNPRTG--MESLTVTPCSKASANQRAGRAGRVAAGKCFRLYTAWAYQHELEETTVPEIQRTSLGNVVLLLKSLGIHDLMHFDFLDPPPYETLLLALEQLYALGALNHLGELTTSGRKMAELPVDPMLSKMILASEKYSCSEEILTVAAMLSVNNSIFYRPKDKVVHADNARVNFFLPGGDHLVLLNVYTQWAESGYSSQWCYENFVQFRSMRRARDVREQLEGLLERVEVGLSSCQGDYIRVRKAITAGYFYHTARLTRSG-YRTVKQQQTVFIHPNSSLFEQ--QPRWLLYHELVLTTKEFMRQVLEIESSWLLEVAPHYYKAKE 1020          

HSP 2 Score: 285.804 bits (730), Expect = 9.362e-157
Identity = 156/304 (51.32%), Postives = 219/304 (72.04%), Query Frame = 3
Query: 1050 SEQKSKIDDQRKRLPIFKVRRDLIRLIRENQVIVIVGETGSGKTTQLTQYLLEENYHHKGI-IGCTQPXXXXXXXXXXXXXDEVGTKLGDKVGYSIRFEDCKSENTMIKYMTDGIXXXXXXXXXXXXAYSVIIMDEAHERTLNTDILFGLLREIIQRRSDLHLVVTSATMDAEKFSNFFGKCEIFKIPGRTFPVDVIYEKSSILDPVAAAVNKAVDTHLGADSKDGDILVFMSGQEDVEVTCDKIDETLEKVKDTNPPDLKVEPLYSQLSSERQSEIFKINTSGIRRCIVATNIAETSVTLDGI 1958
            ++QK  I   R+ LP+F  R +L+  I  +QV++I GETGSGKTTQ+ QYL EE Y +KG+ I CTQPRRVAA+SVA RVA E+G KLG++VGYSIRFEDC SE T+++YMTDG+LLRE L + DL +YSV+++DEAHERTL+TDILFGL++++ + R +L ++V SATMD  +FS FF    +F+IPGR FPVD+ Y K+   D + A V   +  H+      GDILVF++GQE++E  C+ + +   ++  +   +L V P+Y+ L S+ Q+ IF+    G R+ +VATNIAETS+T++GI
Sbjct:  386 AQQKESIQAVRRSLPVFPFREELLAAIANHQVLIIEGETGSGKTTQIPQYLFEEGYTNKGMKIACTQPRRVAAMSVAARVAREMGVKLGNEVGYSIRFEDCTSERTVLRYMTDGMLLREFLSEPDLASYSVVMVDEAHERTLHTDILFGLIKDVARFRPELKVLVASATMDTARFSTFFDDAPVFRIPGRRFPVDIFYTKAPEADYLEACVVSVLQIHV--TQPPGDILVFLTGQEEIEAACEMLQDRCRRL-GSKIRELLVLPIYANLPSDMQARIFQPTPPGARKVVVATNIAETSLTIEGI 686          
BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. Ensembl Celegans
Match: mog-1 (Probable pre-mRNA-splicing factor ATP-dependent RNA helicase mog-1 [Source:UniProtKB/Swiss-Prot;Acc:P34498])

HSP 1 Score: 491.5 bits (1264), Expect = 0.000e+0
Identity = 238/379 (62.80%), Postives = 287/379 (75.73%), Query Frame = 1
Query: 1960 GMDSLQIYPISQASANQRAGRAGRTGPGICYRLYTINQYENDMLPMTVPEIQRTNLTNVVLLLKSLNVQDLMKFYFMDSPPQDNILNSMYHLWMLGALDAVGNLTELGRTMSEFPLDPSLSKMLITSVKHGCSEEMLTIVSMLSVPAIFSRPTGHQEESDCAHEKFQVAESDHLTLLNVYRLWRESGYSANFCKKYYIHNKSMKRVREVRQQLKEIMIKLKMSLASSG-DFDMIRYCICSAYFNQAARWKGLGEYVNLRSGVVCHLHPTSSLYNMAFNADYVVYHELLMTSKEFMNYVTFVDARWLAELGHMFYSVKEKGQSVTDRMLKEQQTVERMETEMRINEKANLKKLLDNRVIESERRAQSVKETTYSRPRSSR 3093
            GMD+L I+P+SQASANQR GRAGRTGPG CYRLYT  Q+++++L  TVPEIQRTNL NVVLLLKSL V DL+KF+FMD+PPQDN+LNSMY LW LGALD  G LT +GR M EFPLDP+LSKMLI S + GCS+E+LTIVSMLSVPAIF RP G +EE+D   EKFQV ESDHLT LNVY  WR   YSA +C   Y+H K++K+VREVR QLKEIM  LK+ L S+G ++D++R CICSAYF+ AAR KG+GEYVN+R+G+ C LHPTS+L+ M F  DYVVYHEL+MT+KE+M  VT VDA WLAELG MFYS+KE  QS  +  ++  +TVE ME EMR  +K            E ERR +   +  + RP SSR
Sbjct:  746 GMDALSIFPVSQASANQRTGRAGRTGPGQCYRLYTERQFKDELLKSTVPEIQRTNLANVVLLLKSLGVDDLLKFHFMDAPPQDNMLNSMYQLWTLGALDNTGQLTPMGRKMVEFPLDPTLSKMLIMSAEMGCSDEVLTIVSMLSVPAIFFRPKGREEEADAKKEKFQVPESDHLTFLNVYIQWRTHKYSAKWCADNYLHVKALKKVREVRAQLKEIMQDLKLPLISNGSEWDIVRKCICSAYFHNAARLKGIGEYVNVRTGIPCFLHPTSALFGMGFMPDYVVYHELIMTAKEYMQCVTAVDAIWLAELGPMFYSIKESKQSRKELKMESVRTVETMEAEMREAQK------------EMERRKEE-SDKAFKRPESSR 1111          

HSP 2 Score: 480.715 bits (1236), Expect = 0.000e+0
Identity = 272/522 (52.11%), Postives = 365/522 (69.92%), Query Frame = 3
Query:  420 NDKSEIKWSPDKNEEELDREWYLNDGM---EYNPFPNFSDEVLEKKKQQLQRQTSIVAKSRKSARAKQFEETNRAWEESKLINSGAVVRKGFNVEIDNEDTGEDRIHLHVHNILPPFLDGRIVFTKQFEPVIPVKDENSDMAKIAKNGSQMVKNYRDIKEKMKLQKRTLDMTGTNFGKLMGLEKK--ETLDKWDE--TNYKDSMKYCDKITDKIAL-DSEQKSKIDDQRKRLPIFKVRRDLIRLIRENQVIVIVGETGSGKTTQLTQYLLEENYHHKGIIGCTQPXXXXXXXXXXXXXDEVGTKLGDKVGYSIRFEDCKSENTMIKYMTDGIXXXXXXXXXXXXAYSVIIMDEAHERTLNTDILFGLLREIIQRRSDLHLVVTSATMDAEKFSNFF-GKCEIFKIPGRTFPVDVIYEKSSILDPVAAAVNKAVDTHLGADSKDGDILVFMSGQEDVEVTCDKIDETLEKVKDTNPPDLKVEPLYSQLSSERQSEIFKINTSGIRRCIVATNIAETSVTLDGI 1958
            +D+   +W  ++ ++ LDREWY N+G    EYNPF   SDE +EK+++Q Q +T    K R + + +  +  N  WE ++L  SG V        I  ++T E+R+ + V NI+PPFLDGRIVFTKQ +P+IPV D   DMA  A  GS  V+  R+++++ K Q +  ++ G+  G LMG+++K  ET D  D+   NYK+S ++   + D  A+ D   +  I  QR+ LP+F  R+ ++ +IREN V++IVGETGSGKTTQL QYLLE+ +   G+IGCTQPRRVAA+SVA+RVADE+G  LG  VGY+IRFEDC SE T+IKYMTDGILLRE L D  LD YS IIMDEAHER+LNTD+LFGLLRE+I +R+DL L+VTSATMDA+KF++FF G C  F IPGRTFPV++ + ++ + D V AAV +AV  HLG    DGDIL+FM GQED+E TC+ I E L ++ D  PP L V P+YSQL S+ Q++IF+    G+R+ IVATNIAETS+T+DGI
Sbjct:  215 DDEERAQW--EEEQKNLDREWYDNEGAFDDEYNPFNKVSDEFVEKREKQWQEKTQ---KPRLTVKQQAIKRENELWENNRLHRSGVVAMADELSSIFEDETDENRVTILVQNIVPPFLDGRIVFTKQAQPIIPVVDTTCDMAVSAARGSVAVRKRREVEDRKKAQDKHWELAGSKLGNLMGVKEKKDETADPEDDDSGNYKESHQFASHMKDNEAVSDFAMEKSIKQQREYLPVFACRQKMMNVIRENNVVIIVGETGSGKTTQLAQYLLEDGFGDSGLIGCTQPRRVAAMSVARRVADEMGVDLGQDVGYAIRFEDCTSEKTIIKYMTDGILLRECLGDGSLDQYSAIIMDEAHERSLNTDVLFGLLREVIAKRADLKLIVTSATMDADKFADFFGGNCPTFTIPGRTFPVELFHARTPVEDYVDAAVKQAVTIHLGG--MDGDILIFMPGQEDIECTCEMIKEKLGEL-DEAPP-LAVLPIYSQLPSDLQAKIFQRAPGGMRKAIVATNIAETSLTVDGI 727          
BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. Ensembl Celegans
Match: mog-1 (Probable pre-mRNA-splicing factor ATP-dependent RNA helicase mog-1 [Source:UniProtKB/Swiss-Prot;Acc:P34498])

HSP 1 Score: 491.5 bits (1264), Expect = 0.000e+0
Identity = 238/379 (62.80%), Postives = 287/379 (75.73%), Query Frame = 1
Query: 1960 GMDSLQIYPISQASANQRAGRAGRTGPGICYRLYTINQYENDMLPMTVPEIQRTNLTNVVLLLKSLNVQDLMKFYFMDSPPQDNILNSMYHLWMLGALDAVGNLTELGRTMSEFPLDPSLSKMLITSVKHGCSEEMLTIVSMLSVPAIFSRPTGHQEESDCAHEKFQVAESDHLTLLNVYRLWRESGYSANFCKKYYIHNKSMKRVREVRQQLKEIMIKLKMSLASSG-DFDMIRYCICSAYFNQAARWKGLGEYVNLRSGVVCHLHPTSSLYNMAFNADYVVYHELLMTSKEFMNYVTFVDARWLAELGHMFYSVKEKGQSVTDRMLKEQQTVERMETEMRINEKANLKKLLDNRVIESERRAQSVKETTYSRPRSSR 3093
            GMD+L I+P+SQASANQR GRAGRTGPG CYRLYT  Q+++++L  TVPEIQRTNL NVVLLLKSL V DL+KF+FMD+PPQDN+LNSMY LW LGALD  G LT +GR M EFPLDP+LSKMLI S + GCS+E+LTIVSMLSVPAIF RP G +EE+D   EKFQV ESDHLT LNVY  WR   YSA +C   Y+H K++K+VREVR QLKEIM  LK+ L S+G ++D++R CICSAYF+ AAR KG+GEYVN+R+G+ C LHPTS+L+ M F  DYVVYHEL+MT+KE+M  VT VDA WLAELG MFYS+KE  QS  +  ++  +TVE ME EMR  +K            E ERR +   +  + RP SSR
Sbjct:  746 GMDALSIFPVSQASANQRTGRAGRTGPGQCYRLYTERQFKDELLKSTVPEIQRTNLANVVLLLKSLGVDDLLKFHFMDAPPQDNMLNSMYQLWTLGALDNTGQLTPMGRKMVEFPLDPTLSKMLIMSAEMGCSDEVLTIVSMLSVPAIFFRPKGREEEADAKKEKFQVPESDHLTFLNVYIQWRTHKYSAKWCADNYLHVKALKKVREVRAQLKEIMQDLKLPLISNGSEWDIVRKCICSAYFHNAARLKGIGEYVNVRTGIPCFLHPTSALFGMGFMPDYVVYHELIMTAKEYMQCVTAVDAIWLAELGPMFYSIKESKQSRKELKMESVRTVETMEAEMREAQK------------EMERRKEE-SDKAFKRPESSR 1111          

HSP 2 Score: 480.715 bits (1236), Expect = 0.000e+0
Identity = 272/522 (52.11%), Postives = 365/522 (69.92%), Query Frame = 3
Query:  420 NDKSEIKWSPDKNEEELDREWYLNDGM---EYNPFPNFSDEVLEKKKQQLQRQTSIVAKSRKSARAKQFEETNRAWEESKLINSGAVVRKGFNVEIDNEDTGEDRIHLHVHNILPPFLDGRIVFTKQFEPVIPVKDENSDMAKIAKNGSQMVKNYRDIKEKMKLQKRTLDMTGTNFGKLMGLEKK--ETLDKWDE--TNYKDSMKYCDKITDKIAL-DSEQKSKIDDQRKRLPIFKVRRDLIRLIRENQVIVIVGETGSGKTTQLTQYLLEENYHHKGIIGCTQPXXXXXXXXXXXXXDEVGTKLGDKVGYSIRFEDCKSENTMIKYMTDGIXXXXXXXXXXXXAYSVIIMDEAHERTLNTDILFGLLREIIQRRSDLHLVVTSATMDAEKFSNFF-GKCEIFKIPGRTFPVDVIYEKSSILDPVAAAVNKAVDTHLGADSKDGDILVFMSGQEDVEVTCDKIDETLEKVKDTNPPDLKVEPLYSQLSSERQSEIFKINTSGIRRCIVATNIAETSVTLDGI 1958
            +D+   +W  ++ ++ LDREWY N+G    EYNPF   SDE +EK+++Q Q +T    K R + + +  +  N  WE ++L  SG V        I  ++T E+R+ + V NI+PPFLDGRIVFTKQ +P+IPV D   DMA  A  GS  V+  R+++++ K Q +  ++ G+  G LMG+++K  ET D  D+   NYK+S ++   + D  A+ D   +  I  QR+ LP+F  R+ ++ +IREN V++IVGETGSGKTTQL QYLLE+ +   G+IGCTQPRRVAA+SVA+RVADE+G  LG  VGY+IRFEDC SE T+IKYMTDGILLRE L D  LD YS IIMDEAHER+LNTD+LFGLLRE+I +R+DL L+VTSATMDA+KF++FF G C  F IPGRTFPV++ + ++ + D V AAV +AV  HLG    DGDIL+FM GQED+E TC+ I E L ++ D  PP L V P+YSQL S+ Q++IF+    G+R+ IVATNIAETS+T+DGI
Sbjct:  215 DDEERAQW--EEEQKNLDREWYDNEGAFDDEYNPFNKVSDEFVEKREKQWQEKTQ---KPRLTVKQQAIKRENELWENNRLHRSGVVAMADELSSIFEDETDENRVTILVQNIVPPFLDGRIVFTKQAQPIIPVVDTTCDMAVSAARGSVAVRKRREVEDRKKAQDKHWELAGSKLGNLMGVKEKKDETADPEDDDSGNYKESHQFASHMKDNEAVSDFAMEKSIKQQREYLPVFACRQKMMNVIRENNVVIIVGETGSGKTTQLAQYLLEDGFGDSGLIGCTQPRRVAAMSVARRVADEMGVDLGQDVGYAIRFEDCTSEKTIIKYMTDGILLRECLGDGSLDQYSAIIMDEAHERSLNTDVLFGLLREVIAKRADLKLIVTSATMDADKFADFFGGNCPTFTIPGRTFPVELFHARTPVEDYVDAAVKQAVTIHLGG--MDGDILIFMPGQEDIECTCEMIKEKLGEL-DEAPP-LAVLPIYSQLPSDLQAKIFQRAPGGMRKAIVATNIAETSLTVDGI 727          
BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. Ensembl Celegans
Match: ddx-15 (Pre-mRNA-splicing factor ATP-dependent RNA helicase ddx-15 [Source:UniProtKB/Swiss-Prot;Acc:Q20875])

HSP 1 Score: 247.669 bits (631), Expect = 6.519e-132
Identity = 141/317 (44.48%), Postives = 211/317 (66.56%), Query Frame = 3
Query: 1050 SEQKSKIDDQRKRLPIFKVRRDLIRLIRENQVIVIVGETGSGKTTQLTQYLLE--------ENYHHKGIIGCTQPXXXXXXXXXXXXXDEVGTKLGDKVGYSIRFEDCKSENTMIKYMTDGIXXXXXXXXXXXXAYSVIIMDEAHERTLNTDILFGLLREIIQRRSDLHLVVTSATMDAEKFSNFFGKCEIFKIPGRTFPVDVIYEKSSILDPVAAAVNKAVDTHLGADSKDGDILVFMSGQEDVEVTCDKIDETLEKVKDTNPPDLKVEPLYSQLSSERQSEIF------KINTSGIRRCIVATNIAETSVTLDGI 1958
            S +   I ++R +LP+++ +   + L+R NQ I +VGETGSGKTTQ+ Q+ +E        +      ++ CTQPRRVAA+SVA RVA+E+   LG +VGYSIRFEDC SE T++KY TDG+LLRE++    LD Y V+I+DEAHERTL TDIL GL++EI++ R+D+ +V+ SAT+DA KF  +F  C +  +PGRTFPV++ +  ++  D + AA+   +  H+  +  +GDIL+F++GQE++E  C +ID  ++ +   +   L   PLYS L    Q  IF      + N +  R+C+++TNIAETS+T+DG+
Sbjct:   63 SNRYWAIWEKRSQLPVWEYKEKFMELLRNNQCITLVGETGSGKTTQIPQWAVEFMKQQQQGQPPGQARLVACTQPRRVAAMSVATRVAEEMDVVLGQEVGYSIRFEDCISERTVLKYCTDGMLLREAMNSPLLDKYKVLILDEAHERTLATDILMGLIKEIVRNRADIKVVIMSATLDAGKFQRYFEDCPLLSVPGRTFPVEIFFTPNAEKDYLEAAIRTVIQIHM-VEEVEGDILLFLTGQEEIEEACKRIDREIQAL-GADAGALSCIPLYSTLPPAAQQRIFEPAPPNRPNGAISRKCVISTNIAETSLTIDGV 377          

HSP 2 Score: 244.588 bits (623), Expect = 6.519e-132
Identity = 136/341 (39.88%), Postives = 202/341 (59.24%), Query Frame = 1
Query: 1963 MDSLQIYPISQASANQRAGRAGRTGPGICYRLYTINQYENDMLPMTVPEIQRTNLTNVVLLLKSLNVQDLMKFYFMDSPPQDNILNSMYHLWMLGALDAVGNLTELGRTMSEFPLDPSLSKMLITSVKHGCSEEMLTIVSMLSVPAIFSRPTGHQEESDCAHEKFQVAESDHLTLLNVYRLWRESGYSANFCKKYYIHNKSMKRVREVRQQLKEIMIKLKMSLASSGDFDM------IRYCICSAYFNQAARWKGLGEYVNLRSGVVCHLHPTSSLYNMAFNADYVVYHELLMTSKEFMNYVTFVDARWLAELGHMFYSVKEKGQSVTDRMLKE-QQTVER 2964
            ++SL + PIS+ASA QRAGRAGRT PG C+RLYT   Y ++M   T PEI R+NL +VVL LK L  +DL+ F FMD P  + ++ ++  L  L A++  G LTELG  M+EFPLDP L+KMLITS +  CS E+L+I +MLSVP  + RP   + E+D A  +F   + DHLTLLNVY  ++++     +C   +I+ ++MK    VR QL  +M K  +   S+ DF        IR  + + +F Q A  +  G YV ++   + +LHP++ L +     ++ +Y+E ++T+K F+  VT V   WL ++   +Y +       T R L    QT++R
Sbjct:  397 VESLLVCPISKASAMQRAGRAGRTKPGKCFRLYTETAYGSEMQDQTYPEILRSNLGSVVLQLKKLGTEDLVHFDFMDPPAPETLMRALELLNYLQAINDDGELTELGSLMAEFPLDPQLAKMLITSTELNCSNEILSITAMLSVPQCWVRPNEMRTEADEAKARFAHIDGDHLTLLNVYHSFKQNQEDPQWCYDNFINYRTMKTADTVRTQLSRVMDKYNLRRVST-DFKSRDYYLNIRKALVAGFFMQVAHLERSGHYVTVKDNQLVNLHPSTVLDH---KPEWALYNEFVLTTKNFIRTVTDVRPEWLLQIAPQYYDLDNFPDGDTKRKLTTVMQTLQR 733          
BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. Ensembl Celegans
Match: ddx-15 (Pre-mRNA-splicing factor ATP-dependent RNA helicase ddx-15 [Source:UniProtKB/Swiss-Prot;Acc:Q20875])

HSP 1 Score: 247.669 bits (631), Expect = 1.607e-128
Identity = 141/317 (44.48%), Postives = 211/317 (66.56%), Query Frame = 3
Query: 1050 SEQKSKIDDQRKRLPIFKVRRDLIRLIRENQVIVIVGETGSGKTTQLTQYLLE--------ENYHHKGIIGCTQPXXXXXXXXXXXXXDEVGTKLGDKVGYSIRFEDCKSENTMIKYMTDGIXXXXXXXXXXXXAYSVIIMDEAHERTLNTDILFGLLREIIQRRSDLHLVVTSATMDAEKFSNFFGKCEIFKIPGRTFPVDVIYEKSSILDPVAAAVNKAVDTHLGADSKDGDILVFMSGQEDVEVTCDKIDETLEKVKDTNPPDLKVEPLYSQLSSERQSEIF------KINTSGIRRCIVATNIAETSVTLDGI 1958
            S +   I ++R +LP+++ +   + L+R NQ I +VGETGSGKTTQ+ Q+ +E        +      ++ CTQPRRVAA+SVA RVA+E+   LG +VGYSIRFEDC SE T++KY TDG+LLRE++    LD Y V+I+DEAHERTL TDIL GL++EI++ R+D+ +V+ SAT+DA KF  +F  C +  +PGRTFPV++ +  ++  D + AA+   +  H+  +  +GDIL+F++GQE++E  C +ID  ++ +   +   L   PLYS L    Q  IF      + N +  R+C+++TNIAETS+T+DG+
Sbjct:   63 SNRYWAIWEKRSQLPVWEYKEKFMELLRNNQCITLVGETGSGKTTQIPQWAVEFMKQQQQGQPPGQARLVACTQPRRVAAMSVATRVAEEMDVVLGQEVGYSIRFEDCISERTVLKYCTDGMLLREAMNSPLLDKYKVLILDEAHERTLATDILMGLIKEIVRNRADIKVVIMSATLDAGKFQRYFEDCPLLSVPGRTFPVEIFFTPNAEKDYLEAAIRTVIQIHM-VEEVEGDILLFLTGQEEIEEACKRIDREIQAL-GADAGALSCIPLYSTLPPAAQQRIFEPAPPNRPNGAISRKCVISTNIAETSLTIDGV 377          

HSP 2 Score: 233.417 bits (594), Expect = 1.607e-128
Identity = 127/305 (41.64%), Postives = 187/305 (61.31%), Query Frame = 1
Query: 1963 MDSLQIYPISQASANQRAGRAGRTGPGICYRLYTINQYENDMLPMTVPEIQRTNLTNVVLLLKSLNVQDLMKFYFMDSPPQDNILNSMYHLWMLGALDAVGNLTELGRTMSEFPLDPSLSKMLITSVKHGCSEEMLTIVSMLSVPAIFSRPTGHQEESDCAHEKFQVAESDHLTLLNVYRLWRESGYSANFCKKYYIHNKSMKRVREVRQQLKEIMIKLKMSLASSGDFDM------IRYCICSAYFNQAARWKGLGEYVNLRSGVVCHLHPTSSLYNMAFNADYVVYHELLMTSKEFMNYVTFV 2859
            ++SL + PIS+ASA QRAGRAGRT PG C+RLYT   Y ++M   T PEI R+NL +VVL LK L  +DL+ F FMD P  + ++ ++  L  L A++  G LTELG  M+EFPLDP L+KMLITS +  CS E+L+I +MLSVP  + RP   + E+D A  +F   + DHLTLLNVY  ++++     +C   +I+ ++MK    VR QL  +M K  +   S+ DF        IR  + + +F Q A  +  G YV ++   + +LHP++ L +     ++ +Y+E ++T+K F+  VT V
Sbjct:  397 VESLLVCPISKASAMQRAGRAGRTKPGKCFRLYTETAYGSEMQDQTYPEILRSNLGSVVLQLKKLGTEDLVHFDFMDPPAPETLMRALELLNYLQAINDDGELTELGSLMAEFPLDPQLAKMLITSTELNCSNEILSITAMLSVPQCWVRPNEMRTEADEAKARFAHIDGDHLTLLNVYHSFKQNQEDPQWCYDNFINYRTMKTADTVRTQLSRVMDKYNLRRVST-DFKSRDYYLNIRKALVAGFFMQVAHLERSGHYVTVKDNQLVNLHPSTVLDH---KPEWALYNEFVLTTKNFIRTVTDV 697          
BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. Ensembl Celegans
Match: ddx-35 (Probable ATP-dependent RNA helicase DHX35 homolog [Source:UniProtKB/Swiss-Prot;Acc:Q9BKQ8])

HSP 1 Score: 224.557 bits (571), Expect = 2.717e-117
Identity = 143/308 (46.43%), Postives = 197/308 (63.96%), Query Frame = 3
Query: 1068 IDDQRKRLPIFKVRRDLIRLIRENQVIVIVGETGSGKTTQLTQYLLEENYHHKG-IIGCTQPXXXXXXXXXXXXXDEVGTKLGDKVGYSIRFEDCKSENTMIKYMTDGIXXXXXXXXXXXXAYSVIIMDEAHERTLNTDILFGLLREIIQRRSDLHLVVTSATMDAEKFSNFFGKCE----------IFKIPGRTFPVDVIYEKSSILDPVAAAVNKAVDTHLGADSKDGDILVFMSGQEDVEVTCDKIDETLEKVKDTNPPDLKVEPLYSQLSSERQSEIFKINTSGIRRCIVATNIAETSVTLDGI 1958
            I  QR RLPIFK R  ++ +    + I+IVGETG GK+TQ+ Q+LLE  +   G  I  TQPRRVA V++A RVA+E    LG  VGY++RF+D   ++T +K+MTDG+LLRE L D  L  YS+I++DEAHER+ NTDIL GLLR+IIQ R+DL ++V+SAT+DAE F +FF   E          I  + GRT PV V + K+S+ D   +AV+  ++ H       GDILVF++GQ++VE  C+K+ E    +K+ +   L V P Y  L +  Q + F     G R+ +VATNIAE S+T+ GI
Sbjct:   70 IQQQRIRLPIFKNRGHILYMCERYRTIIIVGETGCGKSTQVPQFLLEAGWAADGRQIVITQPRRVAVVTLATRVAEEKDCILGHDVGYTVRFDDVSDKDTKVKFMTDGLLLREILADPLLSKYSIIMIDEAHERSCNTDILLGLLRKIIQIRNDLRIIVSSATLDAELFKDFFEMNETGNSDKDTAGIISVEGRTHPVAVHHTKTSVPDYCQSAVDTVINIH--KHENPGDILVFLTGQDEVEDVCEKLRELAGNLKNCD--RLWVVPCYGALPAREQMKAFDSTPHGTRKVVVATNIAEASITIPGI 373          

HSP 2 Score: 218.779 bits (556), Expect = 2.717e-117
Identity = 118/334 (35.33%), Postives = 195/334 (58.38%), Query Frame = 1
Query: 1960 GMDSLQIYPISQASANQRAGRAGRTGPGICYRLYTINQYENDMLPMTVPEIQRTNLTNVVLLLKSLNVQDLMKFYFMDSPPQDNILNSMYHLWMLGALDAVGNLTE-LGRTMSEFPLDPSLSKMLITSVKHGCSEEMLTIVSMLSVPAIFSRPTGHQEESDCAHEKFQVAESDHLTLLNVYRLWRESGYSANFCKKYYIHNKSMKRVREVRQQLKEIMIKLKMSLASSGDF----DMIRYCICSAYFNQAARWKGLGEYVNLRSGVVCHLHPTSSLYNMAFNADY---VVYHELLMTSKEFMNYVTFVDARWLAELGHMFYSVKEKGQSVTDRM 2937
            G+++L    +S+ASA QRAGRAGR  PG CYRLY  +++E      TVPEIQR  + + +L LK+L VQ++ +F+++  PP   ++N +  L+ LGA+D    LT  LG  M+EFPL P  SK L+ S + GCS EM+TIV+M+ +  +F  P   + ++D   +KF V E DH+T+LNV+  + E+G S  +C  ++++ + + R   VR QL  ++ + ++   SS       + IR C+ + +F+QAA++   G+Y+ ++     +++  SS+    F  DY   V++ E++  S   +  VT ++  WL EL   +Y    +G+    RM
Sbjct:  392 GVETLMRVTVSKASAEQRAGRAGRIRPGKCYRLYPESEFER-FAEGTVPEIQRCQMASTILQLKALGVQNVHRFHYLSPPPSWAMINGLELLYALGAIDETSQLTSPLGLQMAEFPLPPMHSKCLLKSAEFGCSTEMVTIVAMMQIQDVFITPYRQRHQADVIRKKFAVEEGDHMTMLNVFTKFVENGRSKKWCSDHFVNYRGLMRADNVRSQLVRLLKRFEIEKVSSRGLINCSENIRQCLVTGFFSQAAQYHYTGKYMTVKESFPFNMYKGSSIM---FKKDYPKWVIFTEVMQDS---IRDVTVIEPEWLYELAPHYYEFGTEGELAEKRM 718          
BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. Ensembl Fly
Match: l(1)G0007 (gene:FBgn0026713 transcript:FBtr0073900)

HSP 1 Score: 491.5 bits (1264), Expect = 0.000e+0
Identity = 270/515 (52.43%), Postives = 375/515 (72.82%), Query Frame = 3
Query:  450 DKNEEELDREWY-LNDGM--EYNPFPNFSDEVLEKKKQQLQRQTSIVAKSRKSARAKQFEETNRAWEESKLINSGAVVRKGFNVEIDNEDTGEDRIHLHVHNILPPFLDGRIVFTKQFEPVIPVKDENSDMAKIAKNGSQMVKNYRDIKEKMKLQKRTLDMTGTNFGKLMGLEKKETLDKW------DETNYKDSMKYCDKITDKIA---LDSEQKSKIDDQRKRLPIFKVRRDLIRLIRENQVIVIVGETGSGKTTQLTQYLLEENYHHKGIIGCTQPXXXXXXXXXXXXXDEVGTKLGDKVGYSIRFEDCKSENTMIKYMTDGIXXXXXXXXXXXXAYSVIIMDEAHERTLNTDILFGLLREIIQRRSDLHLVVTSATMDAEKFSNFFGKCEIFKIPGRTFPVDVIYEKSSILDPVAAAVNKAVDTHLGADSKDGDILVFMSGQEDVEVTCDKIDETLEKVKDTNPPDLKVEPLYSQLSSERQSEIFKINTSGIRRCIVATNIAETSVTLDGI 1958
            ++ +  LDREWY +++G   E NPF   + E   K+++QL+++ +     R SA+ +Q    N  WE ++++ SG V     N + D E    +R+HL VH+I+PPFLDGRIVFTKQ EPV+PVKD  SDMA +A+ GS +V+NYR+ KE+ K QK+  +++GT  G +MG+++ +  D        D+ +Y+   K+ D + D+      D  +K  I +QR+ LP+F  R++L+ +IREN VI+IVGETGSGKTTQLTQYL E+ Y  +G+IGCTQPRRVAA+SVAKRV+DE+ T+LG+ VGY+IRFEDC SE T+IKYMTDGILLRESLRD +LD+YS IIMDEAHER+L+TD+LFGLLREI+ RR DL L+VTSATMD+ KF+ FFG    F IPGRTFPVDV++ K++  D V +AV +A+  HL  +  +GD+L+FM GQED+EVTC+ ++E L ++   N P L + P+YSQL S+ Q++IF+ ++ G+R+C+VATNIAETS+T+DGI
Sbjct:  315 EEEQRRLDREWYNIDEGYDDENNPFGGPNSEYFRKREEQLEQKRT----KRISAQQRQNNRDNELWERNRMLTSGVVTLISVNDDFDEEAL--ERVHLLVHHIIPPFLDGRIVFTKQPEPVVPVKDPTSDMALLARKGSALVRNYREQKERRKAQKKHWELSGTKLGNIMGVQRPQDEDDMRFDKEKDKADYRKDQKFADHMRDQDTGGKSDFSRKKTISEQRRFLPVFASRQELLNVIRENSVIIIVGETGSGKTTQLTQYLHEDGYSKRGMIGCTQPRRVAAMSVAKRVSDEMDTQLGEDVGYAIRFEDCTSERTVIKYMTDGILLRESLRDPELDSYSAIIMDEAHERSLSTDVLFGLLREIVARRHDLKLIVTSATMDSSKFATFFGNVPTFTIPGRTFPVDVMFSKNTCEDYVESAVKQALQVHLTPN--EGDMLIFMPGQEDIEVTCEVLEERLAEID--NAPALSILPIYSQLPSDLQAKIFQKSSDGLRKCVVATNIAETSLTVDGI 819          

HSP 2 Score: 464.922 bits (1195), Expect = 0.000e+0
Identity = 214/341 (62.76%), Postives = 271/341 (79.47%), Query Frame = 1
Query: 1960 GMDSLQIYPISQASANQRAGRAGRTGPGICYRLYTINQYENDMLPMTVPEIQRTNLTNVVLLLKSLNVQDLMKFYFMDSPPQDNILNSMYHLWMLGALDAVGNLTELGRTMSEFPLDPSLSKMLITSVKHGCSEEMLTIVSMLSVPAIFSRPTGHQEESDCAHEKFQVAESDHLTLLNVYRLWRESGYSANFCKKYYIHNKSMKRVREVRQQLKEIMIKLKMSLASSG-DFDMIRYCICSAYFNQAARWKGLGEYVNLRSGVVCHLHPTSSLYNMAFNADYVVYHELLMTSKEFMNYVTFVDARWLAELGHMFYSVKEKGQSVTDRMLKEQQTVERMETEM 2979
            GMD+LQIYPISQA+ANQR+GRAGRTGPG  YRLYT  QY++++L +TVPEIQRTNL N VLLLKSL V DL++F+FMD PPQDNILNS+Y LW+LGALD  G LT LGR M+EFPLDP   +MLI + + GCS E+L IVSMLSVP+IF RP G ++E+D   EKFQ  ESDHLT LNVY+ WR++ YS+ +C +++IH K+M++VREVRQQLK+IM +  +S+ S G D+D++R CICSAYF QAAR KG+GEYVNLR+G+ CHLHPTS+LY +    DYVVYHEL+MT+KE+M   T VD  WLAELG MF+SVKE G+S  ++  +  + ++ ME +M
Sbjct:  838 GMDALQIYPISQANANQRSGRAGRTGPGQAYRLYTQRQYKDELLALTVPEIQRTNLANTVLLLKSLGVVDLLQFHFMDPPPQDNILNSLYQLWILGALDHTGALTTLGRQMAEFPLDPPQCQMLIVACRMGCSAEVLIIVSMLSVPSIFYRPKGREDEADGVREKFQRPESDHLTYLNVYQQWRQNNYSSTWCNEHFIHIKAMRKVREVRQQLKDIMTQQNLSVISCGIDWDIVRKCICSAYFYQAARLKGIGEYVNLRTGMPCHLHPTSALYGLGTTPDYVVYHELIMTAKEYMQCATAVDGYWLAELGPMFFSVKESGRSGREKKKQAAEHLKEMEEQM 1178          
BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. Ensembl Fly
Match: l(1)G0007 (gene:FBgn0026713 transcript:FBtr0073901)

HSP 1 Score: 463.766 bits (1192), Expect = 4.566e-168
Identity = 214/341 (62.76%), Postives = 271/341 (79.47%), Query Frame = 1
Query: 1960 GMDSLQIYPISQASANQRAGRAGRTGPGICYRLYTINQYENDMLPMTVPEIQRTNLTNVVLLLKSLNVQDLMKFYFMDSPPQDNILNSMYHLWMLGALDAVGNLTELGRTMSEFPLDPSLSKMLITSVKHGCSEEMLTIVSMLSVPAIFSRPTGHQEESDCAHEKFQVAESDHLTLLNVYRLWRESGYSANFCKKYYIHNKSMKRVREVRQQLKEIMIKLKMSLASSG-DFDMIRYCICSAYFNQAARWKGLGEYVNLRSGVVCHLHPTSSLYNMAFNADYVVYHELLMTSKEFMNYVTFVDARWLAELGHMFYSVKEKGQSVTDRMLKEQQTVERMETEM 2979
            GMD+LQIYPISQA+ANQR+GRAGRTGPG  YRLYT  QY++++L +TVPEIQRTNL N VLLLKSL V DL++F+FMD PPQDNILNS+Y LW+LGALD  G LT LGR M+EFPLDP   +MLI + + GCS E+L IVSMLSVP+IF RP G ++E+D   EKFQ  ESDHLT LNVY+ WR++ YS+ +C +++IH K+M++VREVRQQLK+IM +  +S+ S G D+D++R CICSAYF QAAR KG+GEYVNLR+G+ CHLHPTS+LY +    DYVVYHEL+MT+KE+M   T VD  WLAELG MF+SVKE G+S  ++  +  + ++ ME +M
Sbjct:  150 GMDALQIYPISQANANQRSGRAGRTGPGQAYRLYTQRQYKDELLALTVPEIQRTNLANTVLLLKSLGVVDLLQFHFMDPPPQDNILNSLYQLWILGALDHTGALTTLGRQMAEFPLDPPQCQMLIVACRMGCSAEVLIIVSMLSVPSIFYRPKGREDEADGVREKFQRPESDHLTYLNVYQQWRQNNYSSTWCNEHFIHIKAMRKVREVRQQLKDIMTQQNLSVISCGIDWDIVRKCICSAYFYQAARLKGIGEYVNLRTGMPCHLHPTSALYGLGTTPDYVVYHELIMTAKEYMQCATAVDGYWLAELGPMFFSVKESGRSGREKKKQAAEHLKEMEEQM 490          

HSP 2 Score: 149.443 bits (376), Expect = 4.566e-168
Identity = 69/135 (51.11%), Postives = 101/135 (74.81%), Query Frame = 3
Query: 1554 MDAEKFSNFFGKCEIFKIPGRTFPVDVIYEKSSILDPVAAAVNKAVDTHLGADSKDGDILVFMSGQEDVEVTCDKIDETLEKVKDTNPPDLKVEPLYSQLSSERQSEIFKINTSGIRRCIVATNIAETSVTLDGI 1958
            MD+ KF+ FFG    F IPGRTFPVDV++ K++  D V +AV +A+  HL  +  +GD+L+FM GQED+EVTC+ ++E L ++   N P L + P+YSQL S+ Q++IF+ ++ G+R+C+VATNIAETS+T+DGI
Sbjct:    1 MDSSKFATFFGNVPTFTIPGRTFPVDVMFSKNTCEDYVESAVKQALQVHLTPN--EGDMLIFMPGQEDIEVTCEVLEERLAEID--NAPALSILPIYSQLPSDLQAKIFQKSSDGLRKCVVATNIAETSLTVDGI 131          
BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. Ensembl Fly
Match: l(2)37Cb (gene:FBgn0086444 transcript:FBtr0081163)

HSP 1 Score: 290.041 bits (741), Expect = 4.986e-153
Identity = 147/330 (44.55%), Postives = 209/330 (63.33%), Query Frame = 1
Query: 1960 GMDSLQIYPISQASANQRAGRAGRTGPGICYRLYTINQYENDMLPMTVPEIQRTNLTNVVLLLKSLNVQDLMKFYFMDSPPQDNILNSMYHLWMLGALDAVGNLTELGRTMSEFPLDPSLSKMLITSVKHGCSEEMLTIVSMLSV-PAIFSRPTGHQEESDCAHEKFQVAESDHLTLLNVYRLWRESGYSANFCKKYYIHNKSMKRVREVRQQLKEIMIKLKMSLASS-GDFDMIRYCICSAYFNQAARWKGLGEYVNLRSGVVCHLHPTSSLYNMAFNADYVVYHELLMTSKEFMNYVTFVDARWLAELGHMFYSVKEKGQSVTDRMLK 2943
            GM+SL + PIS+ASANQRAGRAGRT PG C+RLYT   Y++++   TVPEIQR NL N VL+LK+L + DL+ F F+D PP + ++ ++  L+ LGAL+  G LT+LGR M+EFP+DP + KML+ S K+ CSEEM+TI +MLSV  AIF RP      +D A + F     DHL+LL VY  W E+ YS  +C + +I  +SMKR R+VR+QL  +M ++++ + S   +   +R    + YF   AR    G Y  ++      +HP SSL+       +V+YHEL+ TSKE+M  V  ++++WL E+   +Y  KE   S   +M K
Sbjct:  558 GMESLMVVPISKASANQRAGRAGRTAPGKCFRLYTAWAYKHELEDNTVPEIQRINLGNAVLMLKALGINDLIHFDFLDPPPHETLVLALEQLYALGALNHHGELTKLGRRMAEFPVDPMMGKMLLASEKYKCSEEMVTIAAMLSVNSAIFYRPKDKIIHADTARKNFNHMHGDHLSLLQVYNQWAETDYSTQWCYENFIQYRSMKRARDVREQLVGLMQRVEIDMVSCLPETVNVRKAATAGYFYHVARLSKGGHYKTIKHNQTVMIHPNSSLFEEL--PRWVLYHELVFTSKEYMRQVIEIESKWLLEVAPHYYKAKELEDSTNKKMPK 885          

HSP 2 Score: 273.092 bits (697), Expect = 4.986e-153
Identity = 155/298 (52.01%), Postives = 217/298 (72.82%), Query Frame = 3
Query: 1068 IDDQRKRLPIFKVRRDLIRLIRENQVIVIVGETGSGKTTQLTQYLLEENY-HHKGIIGCTQPXXXXXXXXXXXXXDEVGTKLGDKVGYSIRFEDCKSENTMIKYMTDGIXXXXXXXXXXXXAYSVIIMDEAHERTLNTDILFGLLREIIQRRSDLHLVVTSATMDAEKFSNFFGKCEIFKIPGRTFPVDVIYEKSSILDPVAAAVNKAVDTHLGADSKDGDILVFMSGQEDVEVTCDKIDETLEKVKDTNPPDLKVEPLYSQLSSERQSEIFKINTSGIRRCIVATNIAETSVTLDGI 1958
            +D+ R+ LP++  + DLI  ++E+QV++I GETGSGKTTQ+ QYL+E  +   K +IGCTQPRRVAA+SVA RVA+E+G KLG++VGYSIRFEDC S+ T++KYMTDG L RE L + DL +YSV+I+DEAHERTL+TDILFGL+++I + R +L L+++SAT+DAEKFS FF    IF+IPGR +PVD+ Y K+   D + A     +  H  A    GDILVF++GQ+++E TC ++     K   +   +L V P+Y+ L S+ Q++IF+      R+ I+ATNIAETS+T+D I
Sbjct:  245 LDETRRSLPVYPFKEDLIAAVKEHQVLIIEGETGSGKTTQVPQYLVEAGFTKDKKMIGCTQPRRVAAMSVAARVAEEMGVKLGNEVGYSIRFEDCTSDRTILKYMTDGTLHREFLSEPDLASYSVMIIDEAHERTLHTDILFGLVKDIARFRPELKLLISSATLDAEKFSAFFDDAPIFRIPGRRYPVDIFYTKAPEADYIDACCVSVLQIH--ATQPLGDILVFLTGQDEIE-TCQEVLHDRVKRLGSKIRELIVIPVYANLPSDMQAKIFEPTPPNARKVILATNIAETSLTIDNI 539          
BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. Ensembl Fly
Match: Dhx15 (gene:FBgn0033160 transcript:FBtr0336909)

HSP 1 Score: 263.077 bits (671), Expect = 2.262e-137
Identity = 143/343 (41.69%), Postives = 207/343 (60.35%), Query Frame = 1
Query: 1963 MDSLQIYPISQASANQRAGRAGRTGPGICYRLYTINQYENDMLPMTVPEIQRTNLTNVVLLLKSLNVQDLMKFYFMDSPPQDNILNSMYHLWMLGALDAVGNLTELGRTMSEFPLDPSLSKMLITSVKHGCSEEMLTIVSMLSVPAIFSRPTGHQEESDCAHEKFQVAESDHLTLLNVYRLWRESGYSANFCKKYYIHNKSMKRVREVRQQLKEIMIKLKMSLAS----SGDFDM-IRYCICSAYFNQAARWKGLGEYVNLRSGVVCHLHPTSSLYNMAFNADYVVYHELLMTSKEFMNYVTFVDARWLAELGHMFYSVKEKGQSVTDRMLKEQQTVERMETE 2976
            ++SL + PIS+ASA QR+GRAGRT PG C+RLYT   ++N+M   T PEI R+NL  VVL LK L + DL+ F FMD P  + ++ ++  L  L ALD  GNLT+LG  MSEFPLDP L+KMLI S +H CS E+L+I +MLSVP  F RP   ++ +D A  +F   + DHLTLLNVY  +++S    N+C + +I+ +S+K    VRQQL  IM +  +   S    S D+ + IR  +   +F Q A  +  G Y+ ++      LHP++ L +     D+V+Y+E ++T+K ++  VT V   WL  L   +Y +    Q    R L+  Q  +R+ET+
Sbjct:  386 VESLLVSPISKASAQQRSGRAGRTRPGKCFRLYTEKAFKNEMQDNTYPEILRSNLGTVVLQLKKLGIDDLVHFDFMDPPAPETLMRALELLNYLAALDDDGNLTDLGAVMSEFPLDPQLAKMLIASCQHNCSNEILSITAMLSVPQCFVRPNEAKKAADEAKMRFAHIDGDHLTLLNVYHAFKQSSEDPNWCYENFINFRSLKSADNVRQQLARIMDRFNLRRTSTEFTSKDYYVNIRKALVQGFFMQVAHLERTGYYLTIKDNQNVQLHPSTCLDH---KPDWVIYNEFVLTTKNYIRTVTDVKPEWLCCLAPQYYDLNNFPQCEAKRQLELLQ--QRLETK 723          

HSP 2 Score: 248.054 bits (632), Expect = 2.262e-137
Identity = 148/311 (47.59%), Postives = 216/311 (69.45%), Query Frame = 3
Query: 1050 SEQKSKIDDQRKRLPIFKVRRDLIRLIRENQVIVIVGETGSGKTTQLTQYLLE--ENYHHKGIIGCTQPXXXXXXXXXXXXXDEVGTKLGDKVGYSIRFEDCKSENTMIKYMTDGIXXXXXXXXXXXXAYSVIIMDEAHERTLNTDILFGLLREIIQRRSDLHLVVTSATMDAEKFSNFFGKCEIFKIPGRTFPVDVIYEKSSILDPVAAAVNKAVDTHLGADSKDGDILVFMSGQEDVEVTCDKIDETLEKVKDTNPPDLKVEPLYSQLSSERQSEIFK----INTSGI--RRCIVATNIAETSVTLDGI 1958
            S++   +  +R  LP+F+ + D +RL+  +Q IV+VGETGSGKTTQ+ Q+ ++   +   KG+  CTQPRRVAA+SVA+RV++E+  KLG++VGYSIRFEDC +  T++KYMTDG+LLRE++ D  LD Y VI++DEAHERTL TDIL G+L+E+I++RSDL LVV SAT+DA KF  +F    + K+PGRT PV++ Y      D + AA+   +  H+  +  +GDIL+F++GQE++E  C +I   ++ +  +   +LK  PLYS L    Q  IF+     N +G   R+ +V+TNIAETS+T+DG+
Sbjct:   59 SQRYQNLYKKRIALPVFEYQADFMRLLSLHQCIVLVGETGSGKTTQIPQWCVDFAVSKGRKGV-SCTQPRRVAAMSVAQRVSEEMDVKLGEEVGYSIRFEDCSTAKTLLKYMTDGMLLREAMSDPMLDQYQVILLDEAHERTLATDILMGVLKEVIRQRSDLKLVVMSATLDAGKFQQYFDNAPLMKVPGRTHPVEIFYTPEPERDYLEAAIRTVIQIHM-CEEIEGDILMFLTGQEEIEEACKRIKREIDNL-GSEIGELKCIPLYSTLPPNLQQRIFEPAPPPNANGAIGRKVVVSTNIAETSLTIDGV 366          
BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. Ensembl Fly
Match: Dhx15 (gene:FBgn0033160 transcript:FBtr0088966)

HSP 1 Score: 263.077 bits (671), Expect = 2.262e-137
Identity = 143/343 (41.69%), Postives = 207/343 (60.35%), Query Frame = 1
Query: 1963 MDSLQIYPISQASANQRAGRAGRTGPGICYRLYTINQYENDMLPMTVPEIQRTNLTNVVLLLKSLNVQDLMKFYFMDSPPQDNILNSMYHLWMLGALDAVGNLTELGRTMSEFPLDPSLSKMLITSVKHGCSEEMLTIVSMLSVPAIFSRPTGHQEESDCAHEKFQVAESDHLTLLNVYRLWRESGYSANFCKKYYIHNKSMKRVREVRQQLKEIMIKLKMSLAS----SGDFDM-IRYCICSAYFNQAARWKGLGEYVNLRSGVVCHLHPTSSLYNMAFNADYVVYHELLMTSKEFMNYVTFVDARWLAELGHMFYSVKEKGQSVTDRMLKEQQTVERMETE 2976
            ++SL + PIS+ASA QR+GRAGRT PG C+RLYT   ++N+M   T PEI R+NL  VVL LK L + DL+ F FMD P  + ++ ++  L  L ALD  GNLT+LG  MSEFPLDP L+KMLI S +H CS E+L+I +MLSVP  F RP   ++ +D A  +F   + DHLTLLNVY  +++S    N+C + +I+ +S+K    VRQQL  IM +  +   S    S D+ + IR  +   +F Q A  +  G Y+ ++      LHP++ L +     D+V+Y+E ++T+K ++  VT V   WL  L   +Y +    Q    R L+  Q  +R+ET+
Sbjct:  386 VESLLVSPISKASAQQRSGRAGRTRPGKCFRLYTEKAFKNEMQDNTYPEILRSNLGTVVLQLKKLGIDDLVHFDFMDPPAPETLMRALELLNYLAALDDDGNLTDLGAVMSEFPLDPQLAKMLIASCQHNCSNEILSITAMLSVPQCFVRPNEAKKAADEAKMRFAHIDGDHLTLLNVYHAFKQSSEDPNWCYENFINFRSLKSADNVRQQLARIMDRFNLRRTSTEFTSKDYYVNIRKALVQGFFMQVAHLERTGYYLTIKDNQNVQLHPSTCLDH---KPDWVIYNEFVLTTKNYIRTVTDVKPEWLCCLAPQYYDLNNFPQCEAKRQLELLQ--QRLETK 723          

HSP 2 Score: 248.054 bits (632), Expect = 2.262e-137
Identity = 148/311 (47.59%), Postives = 216/311 (69.45%), Query Frame = 3
Query: 1050 SEQKSKIDDQRKRLPIFKVRRDLIRLIRENQVIVIVGETGSGKTTQLTQYLLE--ENYHHKGIIGCTQPXXXXXXXXXXXXXDEVGTKLGDKVGYSIRFEDCKSENTMIKYMTDGIXXXXXXXXXXXXAYSVIIMDEAHERTLNTDILFGLLREIIQRRSDLHLVVTSATMDAEKFSNFFGKCEIFKIPGRTFPVDVIYEKSSILDPVAAAVNKAVDTHLGADSKDGDILVFMSGQEDVEVTCDKIDETLEKVKDTNPPDLKVEPLYSQLSSERQSEIFK----INTSGI--RRCIVATNIAETSVTLDGI 1958
            S++   +  +R  LP+F+ + D +RL+  +Q IV+VGETGSGKTTQ+ Q+ ++   +   KG+  CTQPRRVAA+SVA+RV++E+  KLG++VGYSIRFEDC +  T++KYMTDG+LLRE++ D  LD Y VI++DEAHERTL TDIL G+L+E+I++RSDL LVV SAT+DA KF  +F    + K+PGRT PV++ Y      D + AA+   +  H+  +  +GDIL+F++GQE++E  C +I   ++ +  +   +LK  PLYS L    Q  IF+     N +G   R+ +V+TNIAETS+T+DG+
Sbjct:   59 SQRYQNLYKKRIALPVFEYQADFMRLLSLHQCIVLVGETGSGKTTQIPQWCVDFAVSKGRKGV-SCTQPRRVAAMSVAQRVSEEMDVKLGEEVGYSIRFEDCSTAKTLLKYMTDGMLLREAMSDPMLDQYQVILLDEAHERTLATDILMGVLKEVIRQRSDLKLVVMSATLDAGKFQQYFDNAPLMKVPGRTHPVEIFYTPEPERDYLEAAIRTVIQIHM-CEEIEGDILMFLTGQEEIEEACKRIKREIDNL-GSEIGELKCIPLYSTLPPNLQQRIFEPAPPPNANGAIGRKVVVSTNIAETSLTIDGV 366          
BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. Ensembl Zebrafish
Match: dhx38 (DEAH (Asp-Glu-Ala-His) box polypeptide 38 [Source:ZFIN;Acc:ZDB-GENE-040426-1144])

HSP 1 Score: 500.36 bits (1287), Expect = 1.303e-157
Identity = 305/653 (46.71%), Postives = 418/653 (64.01%), Query Frame = 3
Query:  243 RHKQWEEMTPSTSLRD---------------ETGRYSEIRTPMIDRSIKTPSV--TSWDEDDVTFKRPDTVT--------------SFRRNDKSEIKWSPDKNEEELDREWYLND-GME--YNPFPNFSDEVLEKKKQQLQRQTSIVAKSRKSARAKQFEETNRAWEESKLINSGAVVRKGFNVEIDNEDTGEDRIHLHVHNILPPFLDGRIVFTKQFEPVIPVKDENSDMAKIAKNGSQMVKNYRDIKEKMKLQKRTLDMTGTNFGKLMGLEKKE------TLDKWDETNYKDSMKYCDKITDK--IALDSEQKSKIDDQRKRLPIFKVRRDLIRLIRENQVIVIVGETGSGKTTQLTQYLLEENYHHKGIIGCTQPXXXXXXXXXXXXXDEVGTKLGDKVGYSIRFEDCKSENTMIKYMTDGIXXXXXXXXXXXXAYSVIIMDEAHERTLNTDILFGLLREIIQRRSDLHLVVTSATMDAEKFSNFFGKCEIFKIPGRTFPVDVIYEKSSILDPVAAAVNKAVDTHLGADSKDGDILVFMSGQEDVEVTCDKIDETLEKVKDTNPPDLKVEPLYSQLSSERQSEIFKINTSGIRRCIVATNIAETSVTLDGI----------------RNGLVADISHQSSQC*SKSRAGR 2027
            RH  WE  +P+ S R+                  R S+ + P  D  + TPS     W  D   F     ++               F   D+ E +W  D  + + DR+WY+ D G +  +NP  + SDE ++K++Q LQ+QT    + R SA+ +Q  E N  WE ++++ SG V R    V+ D E+    R+HL VHN++PPFLDGRIVFTKQ EPVIPVKD  SDMA I++ GSQ+V+ +R+ KE+ K Q +  ++ GT  G +MG++KKE       + +  + +Y+   K+ D + +K   + D  +K  + +QR+ LPIF VR+ L+ +IR+N ++++VGETGSGKTTQLTQYL E+ Y   G++GCTQPRRVAA+SVAKRV++E+ + LG++VGY+IRFEDC SE TMIKYMTDGILLRESLR+ DLD YS +IMDEAHER+LNTD+LFGLLRE++ RRSDL L+VTSATMD++KF++FFG   IF IPGRTFPVD+++ K+   D V AAV +A+  HL      GDIL+FM GQED+EVT D+I E L  ++  N P L V P+YSQL S+ Q++IF+    G+R+CIVATNIAETS+T+DGI                R G+ A   +  SQ  +  RAGR
Sbjct:  249 RHSHWESPSPAPSHRENDRSERSQRSVRDSERRDRSSKPQYP-TDTPLPTPSYKYNEWANDRKHFGSTPRLSRGKGEKREEGEGGIQFDDEDEKE-QWEED--QRQADRDWYMMDEGYDEFHNPLTSSSDEYVKKREQILQKQT----QKRISAQKRQINEDNERWETNRMLTSGVVQR--LEVDEDFEEDNAARVHLLVHNLVPPFLDGRIVFTKQPEPVIPVKDATSDMAIISRKGSQLVRRHREQKERKKAQHKHWELAGTKLGDIMGIQKKEDGGDSKAVGEDGKVDYRAEQKFADHMKEKSEASSDFAKKKTLLEQRQYLPIFAVRQQLLNIIRDNNIVIVVGETGSGKTTQLTQYLHEDGYTSYGMVGCTQPRRVAAMSVAKRVSEEMNSNLGEEVGYAIRFEDCTSEKTMIKYMTDGILLRESLRESDLDHYSAVIMDEAHERSLNTDVLFGLLREVVSRRSDLKLIVTSATMDSDKFASFFGNVPIFHIPGRTFPVDILFSKTPQEDYVEAAVKQALQIHLSG--MVGDILIFMPGQEDIEVTSDQIVERLADLE--NAPALAVLPIYSQLPSDLQAKIFQKAPDGVRKCIVATNIAETSLTVDGIMFVVDSGYCKLKVFNPRIGMDALQVYPISQANANQRAGR 887          

HSP 2 Score: 471.085 bits (1211), Expect = 1.277e-146
Identity = 215/346 (62.14%), Postives = 273/346 (78.90%), Query Frame = 1
Query: 1960 GMDSLQIYPISQASANQRAGRAGRTGPGICYRLYTINQYENDMLPMTVPEIQRTNLTNVVLLLKSLNVQDLMKFYFMDSPPQDNILNSMYHLWMLGALDAVGNLTELGRTMSEFPLDPSLSKMLITSVKHGCSEEMLTIVSMLSVPAIFSRPTGHQEESDCAHEKFQVAESDHLTLLNVYRLWRESGYSANFCKKYYIHNKSMKRVREVRQQLKEIMIKLKMSLASSG-DFDMIRYCICSAYFNQAARWKGLGEYVNLRSGVVCHLHPTSSLYNMAFNADYVVYHELLMTSKEFMNYVTFVDARWLAELGHMFYSVKEKGQSVTDRMLKEQQTVERMETEMRINEK 2994
            GMD+LQ+YPISQA+ANQRAGRAGRTGPG CYRLYT + ++N+ML  T+PEIQRTNL NVVLLLKSL VQDL+ F+FMD PP+DN+LNSMY LW+LGALD  G LT  GR M EFPLDP+LSKMLI S    CS ++L IVSMLSVP+IF RP G +EESD   EKF V ESDHLT LNVY  W+ + YS+ +C  ++IH K+M++VREVR QLK+IM++ KM+L S G D+D+IR CIC+AYF+QAA+ KG+GEYVN+R+G+ CHLHPTS+L+ M +  DY++YHEL+MT+KE+M  VT VD  WLAELG MFYS+K  G+S  +   + ++ +  ME EM + ++
Sbjct:  867 GMDALQVYPISQANANQRAGRAGRTGPGQCYRLYTQSAFKNEMLTTTIPEIQRTNLANVVLLLKSLGVQDLLLFHFMDPPPEDNMLNSMYQLWILGALDNTGALTPTGRLMVEFPLDPALSKMLIVSCDMSCSADILIIVSMLSVPSIFYRPKGREEESDQVREKFSVPESDHLTYLNVYLQWKNNNYSSIWCNDHFIHTKAMRKVREVRAQLKDIMVQQKMNLISCGSDWDVIRKCICAAYFHQAAKLKGIGEYVNVRTGMPCHLHPTSALFGMGYTPDYIIYHELVMTTKEYMQCVTAVDGEWLAELGPMFYSIKHAGKSRQENRRRAKEEITNMEEEMSLAQE 1212          
BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. Ensembl Zebrafish
Match: dhx38 (DEAH (Asp-Glu-Ala-His) box polypeptide 38 [Source:ZFIN;Acc:ZDB-GENE-040426-1144])

HSP 1 Score: 500.36 bits (1287), Expect = 1.303e-157
Identity = 305/653 (46.71%), Postives = 418/653 (64.01%), Query Frame = 3
Query:  243 RHKQWEEMTPSTSLRD---------------ETGRYSEIRTPMIDRSIKTPSV--TSWDEDDVTFKRPDTVT--------------SFRRNDKSEIKWSPDKNEEELDREWYLND-GME--YNPFPNFSDEVLEKKKQQLQRQTSIVAKSRKSARAKQFEETNRAWEESKLINSGAVVRKGFNVEIDNEDTGEDRIHLHVHNILPPFLDGRIVFTKQFEPVIPVKDENSDMAKIAKNGSQMVKNYRDIKEKMKLQKRTLDMTGTNFGKLMGLEKKE------TLDKWDETNYKDSMKYCDKITDK--IALDSEQKSKIDDQRKRLPIFKVRRDLIRLIRENQVIVIVGETGSGKTTQLTQYLLEENYHHKGIIGCTQPXXXXXXXXXXXXXDEVGTKLGDKVGYSIRFEDCKSENTMIKYMTDGIXXXXXXXXXXXXAYSVIIMDEAHERTLNTDILFGLLREIIQRRSDLHLVVTSATMDAEKFSNFFGKCEIFKIPGRTFPVDVIYEKSSILDPVAAAVNKAVDTHLGADSKDGDILVFMSGQEDVEVTCDKIDETLEKVKDTNPPDLKVEPLYSQLSSERQSEIFKINTSGIRRCIVATNIAETSVTLDGI----------------RNGLVADISHQSSQC*SKSRAGR 2027
            RH  WE  +P+ S R+                  R S+ + P  D  + TPS     W  D   F     ++               F   D+ E +W  D  + + DR+WY+ D G +  +NP  + SDE ++K++Q LQ+QT    + R SA+ +Q  E N  WE ++++ SG V R    V+ D E+    R+HL VHN++PPFLDGRIVFTKQ EPVIPVKD  SDMA I++ GSQ+V+ +R+ KE+ K Q +  ++ GT  G +MG++KKE       + +  + +Y+   K+ D + +K   + D  +K  + +QR+ LPIF VR+ L+ +IR+N ++++VGETGSGKTTQLTQYL E+ Y   G++GCTQPRRVAA+SVAKRV++E+ + LG++VGY+IRFEDC SE TMIKYMTDGILLRESLR+ DLD YS +IMDEAHER+LNTD+LFGLLRE++ RRSDL L+VTSATMD++KF++FFG   IF IPGRTFPVD+++ K+   D V AAV +A+  HL      GDIL+FM GQED+EVT D+I E L  ++  N P L V P+YSQL S+ Q++IF+    G+R+CIVATNIAETS+T+DGI                R G+ A   +  SQ  +  RAGR
Sbjct:  249 RHSHWESPSPAPSHRENDRSERSQRSVRDSERRDRSSKPQYP-TDTPLPTPSYKYNEWANDRKHFGSTPRLSRGKGEKREEGEGGIQFDDEDEKE-QWEED--QRQADRDWYMMDEGYDEFHNPLTSSSDEYVKKREQILQKQT----QKRISAQKRQINEDNERWETNRMLTSGVVQR--LEVDEDFEEDNAARVHLLVHNLVPPFLDGRIVFTKQPEPVIPVKDATSDMAIISRKGSQLVRRHREQKERKKAQHKHWELAGTKLGDIMGIQKKEDGGDSKAVGEDGKVDYRAEQKFADHMKEKSEASSDFAKKKTLLEQRQYLPIFAVRQQLLNIIRDNNIVIVVGETGSGKTTQLTQYLHEDGYTSYGMVGCTQPRRVAAMSVAKRVSEEMNSNLGEEVGYAIRFEDCTSEKTMIKYMTDGILLRESLRESDLDHYSAVIMDEAHERSLNTDVLFGLLREVVSRRSDLKLIVTSATMDSDKFASFFGNVPIFHIPGRTFPVDILFSKTPQEDYVEAAVKQALQIHLSG--MVGDILIFMPGQEDIEVTSDQIVERLADLE--NAPALAVLPIYSQLPSDLQAKIFQKAPDGVRKCIVATNIAETSLTVDGIMFVVDSGYCKLKVFNPRIGMDALQVYPISQANANQRAGR 887          

HSP 2 Score: 471.085 bits (1211), Expect = 1.277e-146
Identity = 215/346 (62.14%), Postives = 273/346 (78.90%), Query Frame = 1
Query: 1960 GMDSLQIYPISQASANQRAGRAGRTGPGICYRLYTINQYENDMLPMTVPEIQRTNLTNVVLLLKSLNVQDLMKFYFMDSPPQDNILNSMYHLWMLGALDAVGNLTELGRTMSEFPLDPSLSKMLITSVKHGCSEEMLTIVSMLSVPAIFSRPTGHQEESDCAHEKFQVAESDHLTLLNVYRLWRESGYSANFCKKYYIHNKSMKRVREVRQQLKEIMIKLKMSLASSG-DFDMIRYCICSAYFNQAARWKGLGEYVNLRSGVVCHLHPTSSLYNMAFNADYVVYHELLMTSKEFMNYVTFVDARWLAELGHMFYSVKEKGQSVTDRMLKEQQTVERMETEMRINEK 2994
            GMD+LQ+YPISQA+ANQRAGRAGRTGPG CYRLYT + ++N+ML  T+PEIQRTNL NVVLLLKSL VQDL+ F+FMD PP+DN+LNSMY LW+LGALD  G LT  GR M EFPLDP+LSKMLI S    CS ++L IVSMLSVP+IF RP G +EESD   EKF V ESDHLT LNVY  W+ + YS+ +C  ++IH K+M++VREVR QLK+IM++ KM+L S G D+D+IR CIC+AYF+QAA+ KG+GEYVN+R+G+ CHLHPTS+L+ M +  DY++YHEL+MT+KE+M  VT VD  WLAELG MFYS+K  G+S  +   + ++ +  ME EM + ++
Sbjct:  867 GMDALQVYPISQANANQRAGRAGRTGPGQCYRLYTQSAFKNEMLTTTIPEIQRTNLANVVLLLKSLGVQDLLLFHFMDPPPEDNMLNSMYQLWILGALDNTGALTPTGRLMVEFPLDPALSKMLIVSCDMSCSADILIIVSMLSVPSIFYRPKGREEESDQVREKFSVPESDHLTYLNVYLQWKNNNYSSIWCNDHFIHTKAMRKVREVRAQLKDIMVQQKMNLISCGSDWDVIRKCICAAYFHQAAKLKGIGEYVNVRTGMPCHLHPTSALFGMGYTPDYIIYHELVMTTKEYMQCVTAVDGEWLAELGPMFYSIKHAGKSRQENRRRAKEEITNMEEEMSLAQE 1212          
BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. Ensembl Zebrafish
Match: dhx15 (DEAH (Asp-Glu-Ala-His) box helicase 15 [Source:ZFIN;Acc:ZDB-GENE-030131-650])

HSP 1 Score: 242.662 bits (618), Expect = 1.661e-128
Identity = 132/343 (38.48%), Postives = 205/343 (59.77%), Query Frame = 1
Query: 1963 MDSLQIYPISQASANQRAGRAGRTGPGICYRLYTINQYENDMLPMTVPEIQRTNLTNVVLLLKSLNVQDLMKFYFMDSPPQDNILNSMYHLWMLGALDAVGNLTELGRTMSEFPLDPSLSKMLITSVKHGCSEEMLTIVSMLSVPAIFSRPTGHQEESDCAHEKFQVAESDHLTLLNVYRLWRESGYSANFCKKYYIHNKSMKRVREVRQQLKEIMIKLKMSLAS----SGDFDM-IRYCICSAYFNQAARWKGLGEYVNLRSGVVCHLHPTSSLYNMAFNADYVVYHELLMTSKEFMNYVTFVDARWLAELGHMFYSVKEKGQSVTDRMLKEQQTVERMETE 2976
            ++SL +  IS+ASA QRAGRAGRT PG C+RLYT   Y+ +M   T PEI R+NL +VVL LK L + DL+ F FMD P  + ++ ++  L  L AL+  G+LTELG  M+EFPLDP L+KM+I S    CS E+L+I +MLSVP  F RPT  ++ +D +  +F   + DHLTLLNVY  ++++  S  +C   +I+ +S+     VRQQL  IM +  +   S    S D+ + IR  + + +F Q A  +  G Y+ ++   V  LHP++ L +     ++V+Y+E ++T+K ++   T +   WL ++   +Y +    Q    R L  ++ V +++T+
Sbjct:  427 VESLLVTAISKASAQQRAGRAGRTRPGKCFRLYTEKAYKTEMQDNTYPEILRSNLGSVVLQLKKLGIDDLVHFDFMDPPAPETLMRALELLNYLAALNDDGDLTELGSMMAEFPLDPQLAKMVIASCDFNCSNEVLSITAMLSVPQCFVRPTEAKKAADESKMRFAHIDGDHLTLLNVYHAFKQNHESVQWCYDNFINYRSLMSADNVRQQLSRIMDRFNLPRRSTEFTSRDYYINIRRALVTGFFMQVAHLERTGHYLTVKDNQVVQLHPSTVLDH---KPEWVLYNEFVLTTKNYIRTCTDIKPEWLVKIAPQYYEMSNFPQCEAKRQL--ERIVAKLQTK 764          

HSP 2 Score: 238.81 bits (608), Expect = 1.661e-128
Identity = 142/309 (45.95%), Postives = 209/309 (67.64%), Query Frame = 3
Query: 1065 KIDDQRKRLPIFKVRRDLIRLIRENQVIVIVGETGSGKTTQLTQYLLEENYHHKGI---IGCTQPXXXXXXXXXXXXXDEVGTKLGDKVGYSIRFEDCKSENTMIKYMTDGIXXXXXXXXXXXXAYSVIIMDEAHERTLNTDILFGLLREIIQRRSDLHLVVTSATMDAEKFSNFFGKCEIFKIPGRTFPVDVIYEKSSILDPVAAAVNKAVDTHLGADSKDGDILVFMSGQEDVEVTCDKIDETLEKVKDTNP--PDLKVEPLYSQLSSERQSEIF------KINTSGIRRCIVATNIAETSVTLDGI 1958
            +I  +R +LP+++ +     ++  +Q  V+VGETGSGKTTQ+ Q+ ++      G    + CTQPRRVAA+SVA+RVADE+   LG +VGYSIRFEDC S  T++KYMTDG+LLRE++ D  L+ Y VII+DEAHERTL TDIL G+L+E++++RSDL ++V SAT+DA KF  +F  C +  IPGRT PV++ Y      D + AA+   +  H+  +  +GD+L+F++GQE+++  C +I     ++ D  P   D+K+ PLYS L  ++Q  IF      K N +  R+ +V+TNIAETS+T+DG+
Sbjct:  103 EILKKRLQLPVWEYKERFNDILNRHQSFVLVGETGSGKTTQIPQWCVDMVRSLPGPKRGVACTQPRRVAAMSVAQRVADEMDVMLGQEVGYSIRFEDCSSAKTILKYMTDGMLLREAMNDPLLERYGVIILDEAHERTLATDILMGVLKEVVRQRSDLKVIVMSATLDAGKFQVYFDNCPLLTIPGRTHPVEIFYTPEPERDYLEAAIRTVIQIHM-CEEDEGDVLLFLTGQEEIDEACKRIK---REIDDLGPDVGDIKIIPLYSTLPPQQQQRIFEPPPPRKPNGAIGRKVVVSTNIAETSLTIDGV 407          
BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. Ensembl Zebrafish
Match: dhx33 (DEAH (Asp-Glu-Ala-His) box polypeptide 33 [Source:NCBI gene;Acc:796505])

HSP 1 Score: 225.328 bits (573), Expect = 1.559e-116
Identity = 122/320 (38.12%), Postives = 194/320 (60.62%), Query Frame = 1
Query: 1960 GMDSLQIYPISQASANQRAGRAGRTGPGICYRLYTINQYENDMLPMTVPEIQRTNLTNVVLLLKSLNVQDLMKFYFMDSPPQDNILNSMYHLWMLGALDAVGN---LTELGRTMSEFPLDPSLSKMLITSVKHGCSEEMLTIVSMLSVPAIFSRPTGHQEESDCAHEKFQVAESDHLTLLNVYRLWRESGYSANFCKKYYIHNKSMKRVREVRQQLKEIMIKLKMSLASS-GDFDMIRYCICSAYFNQAARWKGLGEYVNLRSGVVCHLHPTSSLYNMAFNADYVVYHELLMTSKEFMNYVTFVDARWLAELGHMFYSVK 2907
            G++ L +  +S+A A QRAGRAGR   G CYRLYT +++EN +  MTVPEIQR NL +VVL L +L V D++ F F+  P  +++  ++  L +LGA+D   +   LT LG+ M+ FPL+P  SK L+ S    C+EE+LTI+S+LSV ++   P   ++E     +KF  +E DH+TLLN+YR +++   + ++C++ ++++++M  V EVR QL++I IK+ + L SS  D   +R C+    F  AA  +  G YV L +     +HP+S L+        V+++ELL TS+ +M  +  +   WL E    ++  K
Sbjct:  358 GLEVLAVQRVSKAQAWQRAGRAGREDAGSCYRLYTEDEFEN-LANMTVPEIQRCNLASVVLQLLALGVPDVLNFDFVSKPSPESMRTAVEQLCILGAVDRKDDHVSLTPLGKKMACFPLEPRFSKTLLISPDFSCTEEILTIISLLSVDSVLYNPPARRDEVISVRKKFISSEGDHITLLNIYRAFKKVSGNKDWCRENFVNSRNMGLVGEVRAQLRDICIKMNLKLESSVCDLANVRRCLAHGLFMSAAELQPDGSYVALDTHQPVSIHPSSVLFQA--RPVCVLFNELLHTSRCYMRDLCLIQTDWLHEAAPEYFRRK 674          

HSP 2 Score: 216.468 bits (550), Expect = 1.559e-116
Identity = 133/305 (43.61%), Postives = 194/305 (63.61%), Query Frame = 3
Query: 1062 SKIDDQRKRLPIFKVRRDLIRLIRENQVIVIVGETGSGKTTQLTQYLLEENYHHKGIIGCTQPXXXXXXXXXXXXXDEVGTKLGDKVGYSIRFEDCKSENTMIKYMTDGIXXXXXXXXXXXXAYSVIIMDEAHERTLNTDILFGLLREIIQRRSD-----LHLVVTSATMDAEKFSNFFGKCEIFKIPGRTFPVDVIYEKSSILDPVAAAVNKAVDTHLGADSKDGDILVFMSGQEDVEVTCDKIDETLEKVKDTNPPDLKVEPLYSQLSSERQSEIFKINTSGIRRCIVATNIAETSVTLDGIR 1961
            + ++ QR+ LPI++ R  +I  +R+    V +GETGSGKTTQ+ QYL E     +GII  TQPRRVAA+S+A RVA+E   +LG  VGY++RFED  S  T +K+MTDG+LLRE++ D  L  Y+V+I+DEAHERT++TD+LFG+++   +RR +     L +VV SATMD + FS +F K  +  + GR  P+ + Y K    D + AA+      H  A S   DILVF++GQE++E       +  + + DT+   + V PLY+ L   +Q  +F     G R+ I++TNIAETS+T+ GI+
Sbjct:   38 AGVEAQRRHLPIYQSRTQVISQLRQLHSAVFIGETGSGKTTQIPQYLYEAGIGRQGIIAITQPRRVAAISLAGRVAEEKKVQLGKLVGYTVRFEDVTSPETKLKFMTDGMLLREAIGDPLLLRYTVVILDEAHERTVHTDVLFGVVKAAQRRRLEQNKIPLKVVVMSATMDVDLFSQYFNKSPVLYLEGRQHPIQIYYTKQPQSDYLQAALVSIFQIHQEAPSSH-DILVFLTGQEEIEALARTCRDISKHLPDTS-GSMTVVPLYASLPPAQQMRVFLPAPKGSRKVILSTNIAETSITISGIK 340          
BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. Ensembl Zebrafish
Match: AL833789.3 (DEAH (Asp-Glu-Ala-His) box polypeptide 8 [Source:NCBI gene;Acc:606595])

HSP 1 Score: 315.079 bits (806), Expect = 2.568e-90
Identity = 158/338 (46.75%), Postives = 218/338 (64.50%), Query Frame = 1
Query: 1960 GMDSLQIYPISQASANQRAGRAGRTGPGICYRLYTINQYENDMLPMTVPEIQRTNLTNVVLLLKSLNVQDLMKFYFMDSPPQDNILNSMYHLWMLGALDAVGNLTELGRTMSEFPLDPSLSKMLITSVKHGCSEEMLTIVSMLSVPAIFSRPTGHQEESDCAHEKFQVAESDHLTLLNVYRLWRESGYSANFCKKYYIHNKSMKRVREVRQQLKEIMIKLKMSLASSGDFDM-IRYCICSAYFNQAARWKGLGEYVNLRSGVVCHLHPTSSLYNMAFNADYVVYHELLMTSKEFMNYVTFVDARWLAELGHMFYSVKEKGQSVTDRMLKEQQTVERME 2970
            G+D L + PISQA A QRAGRAGRTGPG CYRLYT   Y ++ML   VPEIQRTNL + VL LK++ + DL+ F FMD+PP + ++ +M  L+ LGALD  G LT LGR M+EFPL+P L KMLI SV  GCSEEMLTIVSMLSV  +F RP   Q  +D    KF   E DHLTLL VY  W+ + +S  +C + +I  +S++R +++R+Q+  IM + K+ + S G   + ++  ICS +F  AA+      Y  L    V ++HP+S+L+N     ++VVYHEL++T+KE+M  VT +D RWL E    F+ V +  +      L +Q+  +R+E
Sbjct:  807 GIDQLVVTPISQAQAKQRAGRAGRTGPGKCYRLYTERAYRDEMLTTNVPEIQRTNLASTVLSLKAMGINDLLSFDFMDAPPMETLITAMEQLYTLGALDDEGLLTRLGRRMAEFPLEPMLCKMLIMSVHLGCSEEMLTIVSMLSVQNVFYRPKDKQALADQKKAKFHQPEGDHLTLLAVYNSWKNNKFSNPWCYENFIQARSLRRAQDIRKQMLGIMDRHKLDVVSCGKATVRVQKAICSGFFRNAAKKDPQEGYRTLIDQQVVYIHPSSALFNR--QPEWVVYHELVLTTKEYMREVTTIDPRWLVEFAPAFFKVSDPTR------LSKQKKQQRLE 1136          

HSP 2 Score: 304.679 bits (779), Expect = 9.821e-87
Identity = 194/458 (42.36%), Postives = 280/458 (61.14%), Query Frame = 3
Query:  753 PPFLDGRIVFTKQFEPVIPVKDENSDMAKIAKNGSQMVKNYRDIKEKMKLQKRTLDMTGTNFGKLMGLEKK--ETLDKWDETNYKDSMKYCDKITDKI------ALDSEQKS-------KIDDQRKRLPIFKVRRDLIRLIRENQVIVIVGETGSGKTTQLTQYLLEENYHHKGIIGCTQPXXXXXXXXXXXXXDEVGTKLGDKVGYSIRFEDCKSENTMIKYMTDGIXXXXXXXXXXXXAYSVIIMDEAHERTLNTDILFGLLREIIQRRSDLHLVVTSATMDAEKFSNFFGKCEIFKIPGRTFPVDVIYEKSSILDPVAAAVNKAVDTHLGADSKDGDILVFMSGQEDVEVTCDKIDETLEKVKDTNP--PDLKVEPLYSQLSSERQSEIFKINTSGIRRCIVATNIAETSVTLDGI----------------RNGLVADISHQSSQC*SKSRAGR 2027
            PPFL G    +    PV  VK+ +  +++ A   S + K  R++K+     +R  +M        MGL K   + L   D      +M+    + + I      A    + S        I +QR+ LPI+K++  LI+ + +NQ+++++GETGSGKTTQ+TQYL E  Y  +G IGCTQPRRVAA+SVAKRV++E G  LG +VGY+IRFEDC S  T+IKYMTDG+LLRE L D DL  Y++I++DEAHERT++TD+LFGLL++ +Q+R+D+ L+VTSAT+DA KFS +F +  IF IPGRT+PV+V+Y K    D + A++   +  HL      GDILVF++GQE+++  C+ +    E++K   P  P+L + P+YS L SE Q+ IF     G R+ ++ATNIAETS+T+DGI                + G+   +    SQ  +K RAGR
Sbjct:  383 PPFLRGHTKQSMDMSPVKIVKNPDGSLSQAAMMQSALAKERREVKQA----QREAEMDSIP----MGLNKHWVDPLPDVDGRQIAANMRGIGMMPNDIPEWKKHAFGGNKASYGKKTQLSILEQRESLPIYKLKEQLIQAVHDNQILIVIGETGSGKTTQITQYLAEAGYTTRGKIGCTQPRRVAAMSVAKRVSEEYGCCLGQEVGYTIRFEDCTSPETVIKYMTDGMLLRECLIDPDLGQYAIIMLDEAHERTIHTDVLFGLLKKTVQKRTDMKLIVTSATLDAVKFSQYFYEAPIFTIPGRTYPVEVLYTKEPETDYLDASLITVMQIHL--TEPPGDILVFLTGQEEIDTACEIL---YERMKSLGPDVPELIILPVYSALPSEMQTRIFDPAPPGSRKVVIATNIAETSLTIDGIYYVVDPGFVKQKVYNSKTGIDQLVVTPISQAQAKQRAGR 827          
BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. Ensembl Xenopus
Match: DHX38 (DEAH-box helicase 38 [Source:NCBI gene;Acc:734019])

HSP 1 Score: 493.041 bits (1268), Expect = 0.000e+0
Identity = 291/607 (47.94%), Postives = 408/607 (67.22%), Query Frame = 3
Query:  252 QWEEMTPSTSLRD------------ETGRYSEIRTPMIDRSIKTPSV--TSWDEDDVTFKRPDTVTSFR------------RNDKSEIKWSPDKNEEELDREWYLND-GME--YNPFPNFSDEVLEKKKQQLQRQTSIVAKSRKSARAKQFEETNRAWEESKLINSGAVVRKGFNVEIDNEDTGEDRIHLHVHNILPPFLDGRIVFTKQFEPVIPVKDENSDMAKIAKNGSQMVKNYRDIKEKMKLQKRTLDMTGTNFGKLMGLEKKETLDKWDE-------TNYKDSMKYCDKITDK--IALDSEQKSKIDDQRKRLPIFKVRRDLIRLIRENQVIVIVGETGSGKTTQLTQYLLEENYHHKGIIGCTQPXXXXXXXXXXXXXDEVGTKLGDKVGYSIRFEDCKSENTMIKYMTDGIXXXXXXXXXXXXAYSVIIMDEAHERTLNTDILFGLLREIIQRRSDLHLVVTSATMDAEKFSNFFGKCEIFKIPGRTFPVDVIYEKSSILDPVAAAVNKAVDTHLGADSKDGDILVFMSGQEDVEVTCDKIDETLEKVKDTNPPDLKVEPLYSQLSSERQSEIFKINTSGIRRCIVATNIAETSVTLDGI 1958
            QWE  +P+ S RD            + GRYSE      +  + TPS     W  D         ++  R             +++ + +W  D  + + DR+WY+ D G +  +N   + S++ ++K++QQLQ+QTS     R SA+ +Q  E N  WE ++++ SG V R    V+ D E+    ++HL VHN++PPFLDGRIVFTKQ EPVIPV+D  SD+A IA+ GSQ+V+ +R+ KE+ K Q +  ++ GT  G +MG++K+  +D+ D+        +YK   K+ D + ++   + D  +K  + +QR+ LPIF V+++L+ +IR+N ++++VGETGSGKTTQLTQYL E+ Y   G+I CTQPRRVAA+SVAKRV++E+   LG++VGY+IRFEDC SE T+IKYMTDGILLRE LR+ DLD YS +IMDEAHER+LNTD+LFGLLRE++ RRSDL L+VTSATMDA+KF+ FFG   IF IPGRTFPVD+++ K+   D V AAV +A+  HL   +  GDIL+FM GQED+EVT D+I E LE++ D+ PP L V P+YSQL S+ Q++IF+    G+R+CIVATNIAETS+T+DGI
Sbjct:  179 QWESPSPAPSNRDSEKSHRSERETLKQGRYSE------ESPLPTPSYKYNEWASDRRHLGSTPRLSQGRGKQDSDDDKINFMSEEEQRQWEDD--QRQADRDWYMMDEGFDESHNALTSTSEDYVKKREQQLQKQTS----KRVSAQRRQINEDNERWETNRMLTSGVVQR--LEVDEDFEEDNAAKVHLLVHNLVPPFLDGRIVFTKQPEPVIPVRDATSDLAIIARKGSQLVRKHREQKERKKAQHKHWELAGTKLGDIMGVKKQ--VDEPDKPIAEDGAVDYKAEQKFADHMRERSEASSDFSRKKSLMEQRQYLPIFAVQQELLTIIRDNSIVIVVGETGSGKTTQLTQYLHEDGYTDYGMIACTQPRRVAAMSVAKRVSEEMQVSLGEEVGYAIRFEDCTSEKTLIKYMTDGILLRECLREADLDHYSAVIMDEAHERSLNTDVLFGLLREVVTRRSDLKLIVTSATMDADKFATFFGNVPIFYIPGRTFPVDILFSKTPQEDYVEAAVKQALQIHLSGAA--GDILIFMPGQEDIEVTSDQIVERLEEL-DSAPP-LAVLPIYSQLPSDLQAKIFQKAPDGVRKCIVATNIAETSLTVDGI 765          

HSP 2 Score: 471.085 bits (1211), Expect = 0.000e+0
Identity = 215/334 (64.37%), Postives = 269/334 (80.54%), Query Frame = 1
Query: 1960 GMDSLQIYPISQASANQRAGRAGRTGPGICYRLYTINQYENDMLPMTVPEIQRTNLTNVVLLLKSLNVQDLMKFYFMDSPPQDNILNSMYHLWMLGALDAVGNLTELGRTMSEFPLDPSLSKMLITSVKHGCSEEMLTIVSMLSVPAIFSRPTGHQEESDCAHEKFQVAESDHLTLLNVYRLWRESGYSANFCKKYYIHNKSMKRVREVRQQLKEIMIKLKMSLASSG-DFDMIRYCICSAYFNQAARWKGLGEYVNLRSGVVCHLHPTSSLYNMAFNADYVVYHELLMTSKEFMNYVTFVDARWLAELGHMFYSVKEKGQSVTDRMLKEQQTV 2958
            GMD+LQIYPISQA+ANQR+GRAGRTGPG CYRLYT + Y+N++L  TVPEIQRTNL+NVVLLLKSL VQDL+ F+FMD PP+DN+LNSMY LW+LGALD  G LT  GR M EFPLDP+LSKMLI S   GCS E+L +VSMLSVPAIF RP G +EESD   EKF V ESDHLT LNV+  W+ + YS+ +C +++IH K+M++VREVR QLK+IM+  +MSL+S G D+D++R CIC+AYF+QAAR KG+GEYVN+R+G+ CHLHPTSSL+ M +  DY+VYHEL+MT+KE+M  VT VD  WLAELG MFYS+K  G++  +   + ++ V
Sbjct:  784 GMDALQIYPISQANANQRSGRAGRTGPGQCYRLYTQSAYKNELLHTTVPEIQRTNLSNVVLLLKSLGVQDLLLFHFMDPPPEDNMLNSMYQLWILGALDNTGALTPTGRLMVEFPLDPALSKMLIVSCDMGCSSEILIVVSMLSVPAIFYRPKGREEESDQVREKFAVPESDHLTYLNVFLQWKNNNYSSGWCNQHFIHAKAMRKVREVRAQLKDIMVSQRMSLSSCGSDWDIVRKCICAAYFHQAARLKGIGEYVNVRTGMPCHLHPTSSLFGMGYTPDYIVYHELVMTTKEYMQCVTAVDGEWLAELGPMFYSIKHAGKTRQENRRRAKEEV 1117          
BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. Ensembl Xenopus
Match: DHX38 (DEAH-box helicase 38 [Source:NCBI gene;Acc:734019])

HSP 1 Score: 491.5 bits (1264), Expect = 0.000e+0
Identity = 291/611 (47.63%), Postives = 407/611 (66.61%), Query Frame = 3
Query:  252 QWEEMTPSTSLRDE-----------------TGRYSEIRTPMIDRSIKTPSV--TSWDEDDVTFKRPDTVTSFR-----------RNDKSEIKWSPDKNEEELDREWYLND-GME--YNPFPNFSDEVLEKKKQQLQRQTSIVAKSRKSARAKQFEETNRAWEESKLINSGAVVRKGFNVEIDNEDTGEDRIHLHVHNILPPFLDGRIVFTKQFEPVIPVKDENSDMAKIAKNGSQMVKNYRDIKEKMKLQKRTLDMTGTNFGKLMGLEKKETLDKWDE-------TNYKDSMKYCDKITDK--IALDSEQKSKIDDQRKRLPIFKVRRDLIRLIRENQVIVIVGETGSGKTTQLTQYLLEENYHHKGIIGCTQPXXXXXXXXXXXXXDEVGTKLGDKVGYSIRFEDCKSENTMIKYMTDGIXXXXXXXXXXXXAYSVIIMDEAHERTLNTDILFGLLREIIQRRSDLHLVVTSATMDAEKFSNFFGKCEIFKIPGRTFPVDVIYEKSSILDPVAAAVNKAVDTHLGADSKDGDILVFMSGQEDVEVTCDKIDETLEKVKDTNPPDLKVEPLYSQLSSERQSEIFKINTSGIRRCIVATNIAETSVTLDGI 1958
            QWE  +P+ S RD                   GRYSE      +  + TPS     W  D         ++  R            +++ + +W  D  + + DR+WY+ D G +  +N   + S++ ++K++QQLQ+QTS     R SA+ +Q  E N  WE ++++ SG V R    V+ D E+    ++HL VHN++PPFLDGRIVFTKQ EPVIPV+D  SD+A IA+ GSQ+V+ +R+ KE+ K Q +  ++ GT  G +MG++K+  +D+ D+        +YK   K+ D + ++   + D  +K  + +QR+ LPIF V+++L+ +IR+N ++++VGETGSGKTTQLTQYL E+ Y   G+I CTQPRRVAA+SVAKRV++E+   LG++VGY+IRFEDC SE T+IKYMTDGILLRE LR+ DLD YS +IMDEAHER+LNTD+LFGLLRE++ RRSDL L+VTSATMDA+KF+ FFG   IF IPGRTFPVD+++ K+   D V AAV +A+  HL   +  GDIL+FM GQED+EVT D+I E LE++ D+ PP L V P+YSQL S+ Q++IF+    G+R+CIVATNIAETS+T+DGI
Sbjct:  219 QWESPSPAPSNRDSEKSHRSERESSRRERSERGRYSE------ESPLPTPSYKYNEWASDRRHLGSTPRLSQGRGKNSDDDKINFMSEEEQRQWEDD--QRQADRDWYMMDEGFDESHNALTSTSEDYVKKREQQLQKQTS----KRVSAQRRQINEDNERWETNRMLTSGVVQR--LEVDEDFEEDNAAKVHLLVHNLVPPFLDGRIVFTKQPEPVIPVRDATSDLAIIARKGSQLVRKHREQKERKKAQHKHWELAGTKLGDIMGVKKQ--VDEPDKPIAEDGAVDYKAEQKFADHMRERSEASSDFSRKKSLMEQRQYLPIFAVQQELLTIIRDNSIVIVVGETGSGKTTQLTQYLHEDGYTDYGMIACTQPRRVAAMSVAKRVSEEMQVSLGEEVGYAIRFEDCTSEKTLIKYMTDGILLRECLREADLDHYSAVIMDEAHERSLNTDVLFGLLREVVTRRSDLKLIVTSATMDADKFATFFGNVPIFYIPGRTFPVDILFSKTPQEDYVEAAVKQALQIHLSGAA--GDILIFMPGQEDIEVTSDQIVERLEEL-DSAPP-LAVLPIYSQLPSDLQAKIFQKAPDGVRKCIVATNIAETSLTVDGI 809          

HSP 2 Score: 471.47 bits (1212), Expect = 0.000e+0
Identity = 215/334 (64.37%), Postives = 269/334 (80.54%), Query Frame = 1
Query: 1960 GMDSLQIYPISQASANQRAGRAGRTGPGICYRLYTINQYENDMLPMTVPEIQRTNLTNVVLLLKSLNVQDLMKFYFMDSPPQDNILNSMYHLWMLGALDAVGNLTELGRTMSEFPLDPSLSKMLITSVKHGCSEEMLTIVSMLSVPAIFSRPTGHQEESDCAHEKFQVAESDHLTLLNVYRLWRESGYSANFCKKYYIHNKSMKRVREVRQQLKEIMIKLKMSLASSG-DFDMIRYCICSAYFNQAARWKGLGEYVNLRSGVVCHLHPTSSLYNMAFNADYVVYHELLMTSKEFMNYVTFVDARWLAELGHMFYSVKEKGQSVTDRMLKEQQTV 2958
            GMD+LQIYPISQA+ANQR+GRAGRTGPG CYRLYT + Y+N++L  TVPEIQRTNL+NVVLLLKSL VQDL+ F+FMD PP+DN+LNSMY LW+LGALD  G LT  GR M EFPLDP+LSKMLI S   GCS E+L +VSMLSVPAIF RP G +EESD   EKF V ESDHLT LNV+  W+ + YS+ +C +++IH K+M++VREVR QLK+IM+  +MSL+S G D+D++R CIC+AYF+QAAR KG+GEYVN+R+G+ CHLHPTSSL+ M +  DY+VYHEL+MT+KE+M  VT VD  WLAELG MFYS+K  G++  +   + ++ V
Sbjct:  828 GMDALQIYPISQANANQRSGRAGRTGPGQCYRLYTQSAYKNELLHTTVPEIQRTNLSNVVLLLKSLGVQDLLLFHFMDPPPEDNMLNSMYQLWILGALDNTGALTPTGRLMVEFPLDPALSKMLIVSCDMGCSSEILIVVSMLSVPAIFYRPKGREEESDQVREKFAVPESDHLTYLNVFLQWKNNNYSSGWCNQHFIHAKAMRKVREVRAQLKDIMVSQRMSLSSCGSDWDIVRKCICAAYFHQAARLKGIGEYVNVRTGMPCHLHPTSSLFGMGYTPDYIVYHELVMTTKEYMQCVTAVDGEWLAELGPMFYSIKHAGKTRQENRRRAKEEV 1161          
BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. Ensembl Xenopus
Match: DHX38 (DEAH-box helicase 38 [Source:NCBI gene;Acc:734019])

HSP 1 Score: 491.5 bits (1264), Expect = 0.000e+0
Identity = 291/612 (47.55%), Postives = 407/612 (66.50%), Query Frame = 3
Query:  252 QWEEMTPSTSLRDE-----------------TGRYSEIRTPMIDRSIKTPSV--TSWDEDDVTFKRPDTVTSFR------------RNDKSEIKWSPDKNEEELDREWYLND-GME--YNPFPNFSDEVLEKKKQQLQRQTSIVAKSRKSARAKQFEETNRAWEESKLINSGAVVRKGFNVEIDNEDTGEDRIHLHVHNILPPFLDGRIVFTKQFEPVIPVKDENSDMAKIAKNGSQMVKNYRDIKEKMKLQKRTLDMTGTNFGKLMGLEKKETLDKWDE-------TNYKDSMKYCDKITDK--IALDSEQKSKIDDQRKRLPIFKVRRDLIRLIRENQVIVIVGETGSGKTTQLTQYLLEENYHHKGIIGCTQPXXXXXXXXXXXXXDEVGTKLGDKVGYSIRFEDCKSENTMIKYMTDGIXXXXXXXXXXXXAYSVIIMDEAHERTLNTDILFGLLREIIQRRSDLHLVVTSATMDAEKFSNFFGKCEIFKIPGRTFPVDVIYEKSSILDPVAAAVNKAVDTHLGADSKDGDILVFMSGQEDVEVTCDKIDETLEKVKDTNPPDLKVEPLYSQLSSERQSEIFKINTSGIRRCIVATNIAETSVTLDGI 1958
            QWE  +P+ S RD                   GRYSE      +  + TPS     W  D         ++  R             +++ + +W  D  + + DR+WY+ D G +  +N   + S++ ++K++QQLQ+QTS     R SA+ +Q  E N  WE ++++ SG V R    V+ D E+    ++HL VHN++PPFLDGRIVFTKQ EPVIPV+D  SD+A IA+ GSQ+V+ +R+ KE+ K Q +  ++ GT  G +MG++K+  +D+ D+        +YK   K+ D + ++   + D  +K  + +QR+ LPIF V+++L+ +IR+N ++++VGETGSGKTTQLTQYL E+ Y   G+I CTQPRRVAA+SVAKRV++E+   LG++VGY+IRFEDC SE T+IKYMTDGILLRE LR+ DLD YS +IMDEAHER+LNTD+LFGLLRE++ RRSDL L+VTSATMDA+KF+ FFG   IF IPGRTFPVD+++ K+   D V AAV +A+  HL   +  GDIL+FM GQED+EVT D+I E LE++ D+ PP L V P+YSQL S+ Q++IF+    G+R+CIVATNIAETS+T+DGI
Sbjct:  211 QWESPSPAPSNRDSEKSHRSERESSRRERSERGRYSE------ESPLPTPSYKYNEWASDRRHLGSTPRLSQGRGKQDSDDDKINFMSEEEQRQWEDD--QRQADRDWYMMDEGFDESHNALTSTSEDYVKKREQQLQKQTS----KRVSAQRRQINEDNERWETNRMLTSGVVQR--LEVDEDFEEDNAAKVHLLVHNLVPPFLDGRIVFTKQPEPVIPVRDATSDLAIIARKGSQLVRKHREQKERKKAQHKHWELAGTKLGDIMGVKKQ--VDEPDKPIAEDGAVDYKAEQKFADHMRERSEASSDFSRKKSLMEQRQYLPIFAVQQELLTIIRDNSIVIVVGETGSGKTTQLTQYLHEDGYTDYGMIACTQPRRVAAMSVAKRVSEEMQVSLGEEVGYAIRFEDCTSEKTLIKYMTDGILLRECLREADLDHYSAVIMDEAHERSLNTDVLFGLLREVVTRRSDLKLIVTSATMDADKFATFFGNVPIFYIPGRTFPVDILFSKTPQEDYVEAAVKQALQIHLSGAA--GDILIFMPGQEDIEVTSDQIVERLEEL-DSAPP-LAVLPIYSQLPSDLQAKIFQKAPDGVRKCIVATNIAETSLTVDGI 802          

HSP 2 Score: 471.085 bits (1211), Expect = 0.000e+0
Identity = 215/334 (64.37%), Postives = 269/334 (80.54%), Query Frame = 1
Query: 1960 GMDSLQIYPISQASANQRAGRAGRTGPGICYRLYTINQYENDMLPMTVPEIQRTNLTNVVLLLKSLNVQDLMKFYFMDSPPQDNILNSMYHLWMLGALDAVGNLTELGRTMSEFPLDPSLSKMLITSVKHGCSEEMLTIVSMLSVPAIFSRPTGHQEESDCAHEKFQVAESDHLTLLNVYRLWRESGYSANFCKKYYIHNKSMKRVREVRQQLKEIMIKLKMSLASSG-DFDMIRYCICSAYFNQAARWKGLGEYVNLRSGVVCHLHPTSSLYNMAFNADYVVYHELLMTSKEFMNYVTFVDARWLAELGHMFYSVKEKGQSVTDRMLKEQQTV 2958
            GMD+LQIYPISQA+ANQR+GRAGRTGPG CYRLYT + Y+N++L  TVPEIQRTNL+NVVLLLKSL VQDL+ F+FMD PP+DN+LNSMY LW+LGALD  G LT  GR M EFPLDP+LSKMLI S   GCS E+L +VSMLSVPAIF RP G +EESD   EKF V ESDHLT LNV+  W+ + YS+ +C +++IH K+M++VREVR QLK+IM+  +MSL+S G D+D++R CIC+AYF+QAAR KG+GEYVN+R+G+ CHLHPTSSL+ M +  DY+VYHEL+MT+KE+M  VT VD  WLAELG MFYS+K  G++  +   + ++ V
Sbjct:  821 GMDALQIYPISQANANQRSGRAGRTGPGQCYRLYTQSAYKNELLHTTVPEIQRTNLSNVVLLLKSLGVQDLLLFHFMDPPPEDNMLNSMYQLWILGALDNTGALTPTGRLMVEFPLDPALSKMLIVSCDMGCSSEILIVVSMLSVPAIFYRPKGREEESDQVREKFAVPESDHLTYLNVFLQWKNNNYSSGWCNQHFIHAKAMRKVREVRAQLKDIMVSQRMSLSSCGSDWDIVRKCICAAYFHQAARLKGIGEYVNVRTGMPCHLHPTSSLFGMGYTPDYIVYHELVMTTKEYMQCVTAVDGEWLAELGPMFYSIKHAGKTRQENRRRAKEEV 1154          
BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. Ensembl Xenopus
Match: DHX38 (DEAH-box helicase 38 [Source:NCBI gene;Acc:734019])

HSP 1 Score: 490.73 bits (1262), Expect = 0.000e+0
Identity = 291/612 (47.55%), Postives = 407/612 (66.50%), Query Frame = 3
Query:  252 QWEEMTPSTSLRDE-----------------TGRYSEIRTPMIDRSIKTPSV--TSWDEDDVTFKRPDTVTSFR------------RNDKSEIKWSPDKNEEELDREWYLND-GME--YNPFPNFSDEVLEKKKQQLQRQTSIVAKSRKSARAKQFEETNRAWEESKLINSGAVVRKGFNVEIDNEDTGEDRIHLHVHNILPPFLDGRIVFTKQFEPVIPVKDENSDMAKIAKNGSQMVKNYRDIKEKMKLQKRTLDMTGTNFGKLMGLEKKETLDKWDE-------TNYKDSMKYCDKITDK--IALDSEQKSKIDDQRKRLPIFKVRRDLIRLIRENQVIVIVGETGSGKTTQLTQYLLEENYHHKGIIGCTQPXXXXXXXXXXXXXDEVGTKLGDKVGYSIRFEDCKSENTMIKYMTDGIXXXXXXXXXXXXAYSVIIMDEAHERTLNTDILFGLLREIIQRRSDLHLVVTSATMDAEKFSNFFGKCEIFKIPGRTFPVDVIYEKSSILDPVAAAVNKAVDTHLGADSKDGDILVFMSGQEDVEVTCDKIDETLEKVKDTNPPDLKVEPLYSQLSSERQSEIFKINTSGIRRCIVATNIAETSVTLDGI 1958
            QWE  +P+ S RD                   GRYSE      +  + TPS     W  D         ++  R             +++ + +W  D  + + DR+WY+ D G +  +N   + S++ ++K++QQLQ+QTS     R SA+ +Q  E N  WE ++++ SG V R    V+ D E+    ++HL VHN++PPFLDGRIVFTKQ EPVIPV+D  SD+A IA+ GSQ+V+ +R+ KE+ K Q +  ++ GT  G +MG++K+  +D+ D+        +YK   K+ D + ++   + D  +K  + +QR+ LPIF V+++L+ +IR+N ++++VGETGSGKTTQLTQYL E+ Y   G+I CTQPRRVAA+SVAKRV++E+   LG++VGY+IRFEDC SE T+IKYMTDGILLRE LR+ DLD YS +IMDEAHER+LNTD+LFGLLRE++ RRSDL L+VTSATMDA+KF+ FFG   IF IPGRTFPVD+++ K+   D V AAV +A+  HL   +  GDIL+FM GQED+EVT D+I E LE++ D+ PP L V P+YSQL S+ Q++IF+    G+R+CIVATNIAETS+T+DGI
Sbjct:  211 QWESPSPAPSNRDSEKSHRSERESSRRERSERGRYSE------ESPLPTPSYKYNEWASDRRHLGSTPRLSQGRGKQDSDDDKINFMSEEEQRQWEDD--QRQADRDWYMMDEGFDESHNALTSTSEDYVKKREQQLQKQTS----KRVSAQRRQINEDNERWETNRMLTSGVVQR--LEVDEDFEEDNAAKVHLLVHNLVPPFLDGRIVFTKQPEPVIPVRDATSDLAIIARKGSQLVRKHREQKERKKAQHKHWELAGTKLGDIMGVKKQ--VDEPDKPIAEDGAVDYKAEQKFADHMRERSEASSDFSRKKSLMEQRQYLPIFAVQQELLTIIRDNSIVIVVGETGSGKTTQLTQYLHEDGYTDYGMIACTQPRRVAAMSVAKRVSEEMQVSLGEEVGYAIRFEDCTSEKTLIKYMTDGILLRECLREADLDHYSAVIMDEAHERSLNTDVLFGLLREVVTRRSDLKLIVTSATMDADKFATFFGNVPIFYIPGRTFPVDILFSKTPQEDYVEAAVKQALQIHLSGAA--GDILIFMPGQEDIEVTSDQIVERLEEL-DSAPP-LAVLPIYSQLPSDLQAKIFQKAPDGVRKCIVATNIAETSLTVDGI 802          

HSP 2 Score: 471.085 bits (1211), Expect = 0.000e+0
Identity = 215/334 (64.37%), Postives = 269/334 (80.54%), Query Frame = 1
Query: 1960 GMDSLQIYPISQASANQRAGRAGRTGPGICYRLYTINQYENDMLPMTVPEIQRTNLTNVVLLLKSLNVQDLMKFYFMDSPPQDNILNSMYHLWMLGALDAVGNLTELGRTMSEFPLDPSLSKMLITSVKHGCSEEMLTIVSMLSVPAIFSRPTGHQEESDCAHEKFQVAESDHLTLLNVYRLWRESGYSANFCKKYYIHNKSMKRVREVRQQLKEIMIKLKMSLASSG-DFDMIRYCICSAYFNQAARWKGLGEYVNLRSGVVCHLHPTSSLYNMAFNADYVVYHELLMTSKEFMNYVTFVDARWLAELGHMFYSVKEKGQSVTDRMLKEQQTV 2958
            GMD+LQIYPISQA+ANQR+GRAGRTGPG CYRLYT + Y+N++L  TVPEIQRTNL+NVVLLLKSL VQDL+ F+FMD PP+DN+LNSMY LW+LGALD  G LT  GR M EFPLDP+LSKMLI S   GCS E+L +VSMLSVPAIF RP G +EESD   EKF V ESDHLT LNV+  W+ + YS+ +C +++IH K+M++VREVR QLK+IM+  +MSL+S G D+D++R CIC+AYF+QAAR KG+GEYVN+R+G+ CHLHPTSSL+ M +  DY+VYHEL+MT+KE+M  VT VD  WLAELG MFYS+K  G++  +   + ++ V
Sbjct:  821 GMDALQIYPISQANANQRSGRAGRTGPGQCYRLYTQSAYKNELLHTTVPEIQRTNLSNVVLLLKSLGVQDLLLFHFMDPPPEDNMLNSMYQLWILGALDNTGALTPTGRLMVEFPLDPALSKMLIVSCDMGCSSEILIVVSMLSVPAIFYRPKGREEESDQVREKFAVPESDHLTYLNVFLQWKNNNYSSGWCNQHFIHAKAMRKVREVRAQLKDIMVSQRMSLSSCGSDWDIVRKCICAAYFHQAARLKGIGEYVNVRTGMPCHLHPTSSLFGMGYTPDYIVYHELVMTTKEYMQCVTAVDGEWLAELGPMFYSIKHAGKTRQENRRRAKEEV 1154          
BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. Ensembl Xenopus
Match: DHX38 (DEAH-box helicase 38 [Source:NCBI gene;Acc:734019])

HSP 1 Score: 490.73 bits (1262), Expect = 0.000e+0
Identity = 291/611 (47.63%), Postives = 407/611 (66.61%), Query Frame = 3
Query:  252 QWEEMTPSTSLRDE-----------------TGRYSEIRTPMIDRSIKTPSV--TSWDEDDVTFKRPDTVTSFR-----------RNDKSEIKWSPDKNEEELDREWYLND-GME--YNPFPNFSDEVLEKKKQQLQRQTSIVAKSRKSARAKQFEETNRAWEESKLINSGAVVRKGFNVEIDNEDTGEDRIHLHVHNILPPFLDGRIVFTKQFEPVIPVKDENSDMAKIAKNGSQMVKNYRDIKEKMKLQKRTLDMTGTNFGKLMGLEKKETLDKWDE-------TNYKDSMKYCDKITDK--IALDSEQKSKIDDQRKRLPIFKVRRDLIRLIRENQVIVIVGETGSGKTTQLTQYLLEENYHHKGIIGCTQPXXXXXXXXXXXXXDEVGTKLGDKVGYSIRFEDCKSENTMIKYMTDGIXXXXXXXXXXXXAYSVIIMDEAHERTLNTDILFGLLREIIQRRSDLHLVVTSATMDAEKFSNFFGKCEIFKIPGRTFPVDVIYEKSSILDPVAAAVNKAVDTHLGADSKDGDILVFMSGQEDVEVTCDKIDETLEKVKDTNPPDLKVEPLYSQLSSERQSEIFKINTSGIRRCIVATNIAETSVTLDGI 1958
            QWE  +P+ S RD                   GRYSE      +  + TPS     W  D         ++  R            +++ + +W  D  + + DR+WY+ D G +  +N   + S++ ++K++QQLQ+QTS     R SA+ +Q  E N  WE ++++ SG V R    V+ D E+    ++HL VHN++PPFLDGRIVFTKQ EPVIPV+D  SD+A IA+ GSQ+V+ +R+ KE+ K Q +  ++ GT  G +MG++K+  +D+ D+        +YK   K+ D + ++   + D  +K  + +QR+ LPIF V+++L+ +IR+N ++++VGETGSGKTTQLTQYL E+ Y   G+I CTQPRRVAA+SVAKRV++E+   LG++VGY+IRFEDC SE T+IKYMTDGILLRE LR+ DLD YS +IMDEAHER+LNTD+LFGLLRE++ RRSDL L+VTSATMDA+KF+ FFG   IF IPGRTFPVD+++ K+   D V AAV +A+  HL   +  GDIL+FM GQED+EVT D+I E LE++ D+ PP L V P+YSQL S+ Q++IF+    G+R+CIVATNIAETS+T+DGI
Sbjct:  219 QWESPSPAPSNRDSEKSHRSERESSRRERSERGRYSE------ESPLPTPSYKYNEWASDRRHLGSTPRLSQGRGKNSDDDKINFMSEEEQRQWEDD--QRQADRDWYMMDEGFDESHNALTSTSEDYVKKREQQLQKQTS----KRVSAQRRQINEDNERWETNRMLTSGVVQR--LEVDEDFEEDNAAKVHLLVHNLVPPFLDGRIVFTKQPEPVIPVRDATSDLAIIARKGSQLVRKHREQKERKKAQHKHWELAGTKLGDIMGVKKQ--VDEPDKPIAEDGAVDYKAEQKFADHMRERSEASSDFSRKKSLMEQRQYLPIFAVQQELLTIIRDNSIVIVVGETGSGKTTQLTQYLHEDGYTDYGMIACTQPRRVAAMSVAKRVSEEMQVSLGEEVGYAIRFEDCTSEKTLIKYMTDGILLRECLREADLDHYSAVIMDEAHERSLNTDVLFGLLREVVTRRSDLKLIVTSATMDADKFATFFGNVPIFYIPGRTFPVDILFSKTPQEDYVEAAVKQALQIHLSGAA--GDILIFMPGQEDIEVTSDQIVERLEEL-DSAPP-LAVLPIYSQLPSDLQAKIFQKAPDGVRKCIVATNIAETSLTVDGI 809          

HSP 2 Score: 471.085 bits (1211), Expect = 0.000e+0
Identity = 215/334 (64.37%), Postives = 269/334 (80.54%), Query Frame = 1
Query: 1960 GMDSLQIYPISQASANQRAGRAGRTGPGICYRLYTINQYENDMLPMTVPEIQRTNLTNVVLLLKSLNVQDLMKFYFMDSPPQDNILNSMYHLWMLGALDAVGNLTELGRTMSEFPLDPSLSKMLITSVKHGCSEEMLTIVSMLSVPAIFSRPTGHQEESDCAHEKFQVAESDHLTLLNVYRLWRESGYSANFCKKYYIHNKSMKRVREVRQQLKEIMIKLKMSLASSG-DFDMIRYCICSAYFNQAARWKGLGEYVNLRSGVVCHLHPTSSLYNMAFNADYVVYHELLMTSKEFMNYVTFVDARWLAELGHMFYSVKEKGQSVTDRMLKEQQTV 2958
            GMD+LQIYPISQA+ANQR+GRAGRTGPG CYRLYT + Y+N++L  TVPEIQRTNL+NVVLLLKSL VQDL+ F+FMD PP+DN+LNSMY LW+LGALD  G LT  GR M EFPLDP+LSKMLI S   GCS E+L +VSMLSVPAIF RP G +EESD   EKF V ESDHLT LNV+  W+ + YS+ +C +++IH K+M++VREVR QLK+IM+  +MSL+S G D+D++R CIC+AYF+QAAR KG+GEYVN+R+G+ CHLHPTSSL+ M +  DY+VYHEL+MT+KE+M  VT VD  WLAELG MFYS+K  G++  +   + ++ V
Sbjct:  828 GMDALQIYPISQANANQRSGRAGRTGPGQCYRLYTQSAYKNELLHTTVPEIQRTNLSNVVLLLKSLGVQDLLLFHFMDPPPEDNMLNSMYQLWILGALDNTGALTPTGRLMVEFPLDPALSKMLIVSCDMGCSSEILIVVSMLSVPAIFYRPKGREEESDQVREKFAVPESDHLTYLNVFLQWKNNNYSSGWCNQHFIHAKAMRKVREVRAQLKDIMVSQRMSLSSCGSDWDIVRKCICAAYFHQAARLKGIGEYVNVRTGMPCHLHPTSSLFGMGYTPDYIVYHELVMTTKEYMQCVTAVDGEWLAELGPMFYSIKHAGKTRQENRRRAKEEV 1161          
BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. Ensembl Mouse
Match: Dhx38 (DEAH (Asp-Glu-Ala-His) box polypeptide 38 [Source:MGI Symbol;Acc:MGI:1927617])

HSP 1 Score: 503.056 bits (1294), Expect = 0.000e+0
Identity = 300/604 (49.67%), Postives = 405/604 (67.05%), Query Frame = 3
Query:  243 RHKQWEEMTPSTSLRD-ETGRYSEIRTPMIDRSIK----------TPSV--TSWDEDDVTFKRPDTVTSFR-RNDKSEIKWSPDKNEE---------ELDREWYLND-GME--YNPFPNFSDEVLEKKKQQLQRQTSIVAKSRKSARAKQFEETNRAWEESKLINSGAVVRKGFNVEIDNEDTGEDRIHLHVHNILPPFLDGRIVFTKQFEPVIPVKDENSDMAKIAKNGSQMVKNYRDIKEKMKLQKRTLDMTGTNFGKLMGLEKKETLDKW----DETNYKDSMKYCDKITDKIALDSE--QKSKIDDQRKRLPIFKVRRDLIRLIRENQVIVIVGETGSGKTTQLTQYLLEENYHHKGIIGCTQPXXXXXXXXXXXXXDEVGTKLGDKVGYSIRFEDCKSENTMIKYMTDGIXXXXXXXXXXXXAYSVIIMDEAHERTLNTDILFGLLREIIQRRSDLHLVVTSATMDAEKFSNFFGKCEIFKIPGRTFPVDVIYEKSSILDPVAAAVNKAVDTHLGADSKDGDILVFMSGQEDVEVTCDKIDETLEKVKDTNPPDLKVEPLYSQLSSERQSEIFKINTSGIRRCIVATNIAETSVTLDGI 1958
            R  QWE  +P+ S RD E G     R    DRSI+          TPS     W +D         ++  R R +  E   + D  EE         + DR+WY+ D G +  +NP    S++ + +++Q L +Q     + R SA+ +Q  E N  WE ++++ SG V R    V+ D E+    ++HL VHN++PPFLDGRIVFTKQ EPVIPVKD  SD+A IA+ GSQ V+ +R+ KE+ K Q +  ++ GT  G +MG++K+E  DK      + +Y+   K+ D + +K    SE  +K  I +QR+ LPIF V+++L+ +IR+N ++++VGETGSGKTTQLTQYL E+ Y   G+IGCTQPRRVAA+SVAKRV++E+G  LG++VGY+IRFEDC SENT+IKYMTDGILLRESLR+ DLD YS +IMDEAHER+LNTD+LFGLLRE++ RRSDL L+VTSATMDAEKF+ FFG   IF IPGRTFPVD+++ K+   D V AAV +++  HL      GDIL+FM GQED+EVT D+I E LE+++  N P L V P+YSQL S+ Q++IF+    G+R+CIVATNIAETS+T+DGI
Sbjct:  227 RRSQWETPSPTPSYRDSERGHRPSTRD--RDRSIRSKSSDDTPLPTPSYKYNEWADDRRHLGSTPRLSRGRGRREDGEEGIAFDTEEERQQWEDDQRQADRDWYMMDEGYDEFHNPLAYSSEDYVRRREQHLHKQK----QKRISAQRRQINEDNERWETNRMLTSGVVHR--LEVDEDFEEDNAAKVHLMVHNLVPPFLDGRIVFTKQPEPVIPVKDATSDLAIIARKGSQTVRKHREQKERRKAQHKHWELAGTKLGDIMGVKKEEEPDKAMTEDGKVDYRTEQKFADHMKEKSEASSEFAKKKSILEQRQYLPIFAVQQELLTIIRDNSIVIVVGETGSGKTTQLTQYLHEDGYTDYGMIGCTQPRRVAAMSVAKRVSEEMGGNLGEEVGYAIRFEDCTSENTLIKYMTDGILLRESLREADLDHYSAVIMDEAHERSLNTDVLFGLLREVVARRSDLKLIVTSATMDAEKFAAFFGNVPIFHIPGRTFPVDILFSKTPQEDYVEAAVKQSLQVHLSG--APGDILIFMPGQEDIEVTSDQIVEHLEELE--NAPALAVLPIYSQLPSDLQAKIFQKAPDGVRKCIVATNIAETSLTVDGI 818          

HSP 2 Score: 474.937 bits (1221), Expect = 0.000e+0
Identity = 217/322 (67.39%), Postives = 264/322 (81.99%), Query Frame = 1
Query: 1960 GMDSLQIYPISQASANQRAGRAGRTGPGICYRLYTINQYENDMLPMTVPEIQRTNLTNVVLLLKSLNVQDLMKFYFMDSPPQDNILNSMYHLWMLGALDAVGNLTELGRTMSEFPLDPSLSKMLITSVKHGCSEEMLTIVSMLSVPAIFSRPTGHQEESDCAHEKFQVAESDHLTLLNVYRLWRESGYSANFCKKYYIHNKSMKRVREVRQQLKEIMIKLKMSLASSG-DFDMIRYCICSAYFNQAARWKGLGEYVNLRSGVVCHLHPTSSLYNMAFNADYVVYHELLMTSKEFMNYVTFVDARWLAELGHMFYSVKEKGQS 2922
            GMD+LQIYPISQA+ANQR+GRAGRTGPG C+RLYT + Y+N++L  TVPEIQRTNL NVVLLLKSL VQDL++F+FMD PP+DN+LNSMY LW+LGALD  G LT  GR M EFPLDP+LSKMLI S   GCS E+L IVSMLSVPAIF RP G +EESD   EKF V ESDHLT LNVY  W+ + YS  +C  ++IH K+M++VREVR QLK+IM++ +MSLAS G D+D++R CIC+AYF+QAA+ KG+GEYVN+R+G+ CHLHPTSSL+ M +  DY+VYHEL+MT+KE+M  VT VD  WLAELG MFYSVK+ G+S
Sbjct:  837 GMDALQIYPISQANANQRSGRAGRTGPGQCFRLYTQSAYKNELLTTTVPEIQRTNLANVVLLLKSLGVQDLLQFHFMDPPPEDNMLNSMYQLWILGALDNTGGLTSTGRLMVEFPLDPALSKMLIVSCDMGCSSEILLIVSMLSVPAIFYRPKGREEESDQIREKFAVPESDHLTYLNVYLQWKNNNYSTIWCNDHFIHAKAMRKVREVRAQLKDIMVQQRMSLASCGTDWDIVRKCICAAYFHQAAKLKGIGEYVNIRTGMPCHLHPTSSLFGMGYTPDYIVYHELVMTTKEYMQCVTAVDGEWLAELGPMFYSVKQAGKS 1158          
BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. Ensembl Mouse
Match: Dhx16 (DEAH (Asp-Glu-Ala-His) box polypeptide 16 [Source:MGI Symbol;Acc:MGI:1916442])

HSP 1 Score: 291.582 bits (745), Expect = 1.326e-156
Identity = 150/328 (45.73%), Postives = 213/328 (64.94%), Query Frame = 1
Query: 1933 KPLSPSTGYGMDSLQIYPISQASANQRAGRAGRTGPGICYRLYTINQYENDMLPMTVPEIQRTNLTNVVLLLKSLNVQDLMKFYFMDSPPQDNILNSMYHLWMLGALDAVGNLTELGRTMSEFPLDPSLSKMLITSVKHGCSEEMLTIVSMLSV-PAIFSRPTGHQEESDCAHEKFQVAESDHLTLLNVYRLWRESGYSANFCKKYYIHNKSMKRVREVRQQLKEIMIKLKMSLAS-SGDFDMIRYCICSAYFNQAARWKGLGEYVNLRSGVVCHLHPTSSLYNMAFNADYVVYHELLMTSKEFMNYVTFVDARWLAELGHMFYSVKE 2910
            K  +P TG  M+SL + P S+ASANQRAGRAGR   G C+RLYT   Y++++   TVPEIQRT+L NVVLLLKSL + DLM F F+D PP + +L ++  L+ LGAL+ +G LT  GR M+E P+DP LSKM++ S K+ CSEE+LT+ +MLSV  +IF RP      +D A   F +   DHL LLNVY  W ESGYS+ +C + ++  +SM+R R+VR+QL+ ++ ++++ L S  GD+  +R  I S YF   AR    G Y  ++      +HP SSL+       +++YHEL++T+KEFM  V  +++ WL E+   +Y  KE
Sbjct:  701 KSYNPRTG--MESLTVTPCSKASANQRAGRAGRVAAGKCFRLYTAWAYQHELEETTVPEIQRTSLGNVVLLLKSLGIHDLMHFDFLDPPPYETLLLALEQLYALGALNHLGELTTSGRKMAELPVDPMLSKMILASEKYSCSEEILTVAAMLSVNNSIFYRPKDKVVHADNARVNFFLPGGDHLVLLNVYTQWAESGYSSQWCYENFVQFRSMRRARDVREQLEGLLERVEVGLTSCQGDYVRVRKAITSGYFYHTARLTRSG-YRTVKQQQTVFIHPNSSLFEQ--QPRWLLYHELVLTTKEFMRQVLEIESSWLLEVAPHYYKAKE 1023          

HSP 2 Score: 283.878 bits (725), Expect = 1.326e-156
Identity = 155/308 (50.32%), Postives = 220/308 (71.43%), Query Frame = 3
Query: 1038 IALDSEQKSKIDDQRKRLPIFKVRRDLIRLIRENQVIVIVGETGSGKTTQLTQYLLEENYHHKGI-IGCTQPXXXXXXXXXXXXXDEVGTKLGDKVGYSIRFEDCKSENTMIKYMTDGIXXXXXXXXXXXXAYSVIIMDEAHERTLNTDILFGLLREIIQRRSDLHLVVTSATMDAEKFSNFFGKCEIFKIPGRTFPVDVIYEKSSILDPVAAAVNKAVDTHLGADSKDGDILVFMSGQEDVEVTCDKIDETLEKVKDTNPPDLKVEPLYSQLSSERQSEIFKINTSGIRRCIVATNIAETSVTLDGI 1958
            ++  ++QK  I   R+ LP+F  R +L+  I  +QV++I GETGSGKTTQ+ QYL EE Y  KG+ I CTQPRRVAA+SVA RVA E+G KLG++VGYSIRFEDC SE T+++YMTDG+LLRE L + DL +YSV+++DEAHERTL+TDILFGL++++ + R +L ++V SAT+D  +FS FF    +F+IPGR FPVD+ Y K+   D + A V   +  H+      GDILVF++GQE++E  C+ + +   ++  +   +L V P+Y+ L S+ Q+ IF+    G R+ +VATNIAETS+T++GI
Sbjct:  385 LSAQAQQKESIQAVRRSLPVFPFREELLAAIANHQVLIIEGETGSGKTTQIPQYLFEEGYTKKGMKIACTQPRRVAAMSVAARVAREMGVKLGNEVGYSIRFEDCTSERTVLRYMTDGMLLREFLSEPDLASYSVVMVDEAHERTLHTDILFGLIKDVARFRPELKVLVASATLDTARFSAFFDDAPVFRIPGRRFPVDIFYTKAPEADYLEACVVSVLQIHV--TQPPGDILVFLTGQEEIEAACEMLQDRCRRL-GSKIRELLVLPIYANLPSDMQARIFQPTPPGARKVVVATNIAETSLTIEGI 689          
BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. Ensembl Mouse
Match: Dhx15 (DEAH (Asp-Glu-Ala-His) box polypeptide 15 [Source:MGI Symbol;Acc:MGI:1099786])

HSP 1 Score: 245.743 bits (626), Expect = 6.506e-130
Identity = 132/332 (39.76%), Postives = 197/332 (59.34%), Query Frame = 1
Query: 1963 MDSLQIYPISQASANQRAGRAGRTGPGICYRLYTINQYENDMLPMTVPEIQRTNLTNVVLLLKSLNVQDLMKFYFMDSPPQDNILNSMYHLWMLGALDAVGNLTELGRTMSEFPLDPSLSKMLITSVKHGCSEEMLTIVSMLSVPAIFSRPTGHQEESDCAHEKFQVAESDHLTLLNVYRLWRESGYSANFCKKYYIHNKSMKRVREVRQQLKEIMIKLKMSLASSGDFDM------IRYCICSAYFNQAARWKGLGEYVNLRSGVVCHLHPTSSLYNMAFNADYVVYHELLMTSKEFMNYVTFVDARWLAELGHMFYSVKEKGQSVTDRML 2940
            ++SL +  IS+ASA QRAGRAGRT PG C+RLYT   Y+ +M   T PEI R+NL +VVL LK L + DL+ F FMD P  + ++ ++  L  L AL+  G+LTELG  M+EFPLDP L+KM+I S  + CS E+L+I +MLSVP  F RPT  ++ +D A  +F   + DHLTLLNVY  ++++  S  +C   +I+ +S+     VRQQL  IM +  +   S+ DF        IR  + + YF Q A  +  G Y+ ++   V  LHP++ L +     ++V+Y+E ++T+K ++   T +   WL ++   +Y +    Q    R L
Sbjct:  453 VESLLVTAISKASAQQRAGRAGRTRPGKCFRLYTEKAYKTEMQDNTYPEILRSNLGSVVLQLKKLGIDDLVHFDFMDPPAPETLMRALELLNYLAALNDDGDLTELGSMMAEFPLDPQLAKMVIASCDYNCSNEVLSITAMLSVPQCFVRPTEAKKAADEAKMRFAHIDGDHLTLLNVYHAFKQNHESVQWCYDNFINYRSLMSADNVRQQLSRIMDRFNLPRRST-DFTSRDYYINIRKALVTGYFMQVAHLERTGHYLTVKDNQVVQLHPSTVLDH---KPEWVLYNEFVLTTKNYIRTCTDIKPEWLVKIAPQYYDMSNFPQCEAKRQL 780          

HSP 2 Score: 240.736 bits (613), Expect = 6.506e-130
Identity = 143/305 (46.89%), Postives = 208/305 (68.20%), Query Frame = 3
Query: 1077 QRKRLPIFKVRRDLIRLIRENQVIVIVGETGSGKTTQLTQYLLEENYHHKGI---IGCTQPXXXXXXXXXXXXXDEVGTKLGDKVGYSIRFEDCKSENTMIKYMTDGIXXXXXXXXXXXXAYSVIIMDEAHERTLNTDILFGLLREIIQRRSDLHLVVTSATMDAEKFSNFFGKCEIFKIPGRTFPVDVIYEKSSILDPVAAAVNKAVDTHLGADSKDGDILVFMSGQEDVEVTCDKIDETLEKVKDTNPP--DLKVEPLYSQLSSERQSEIF------KINTSGIRRCIVATNIAETSVTLDGI 1958
            +R +LP+++ +     ++  +Q  V+VGETGSGKTTQ+ Q+ +E      G    + CTQPRRVAA+SVA+RVADE+   LG +VGYSIRFEDC S  T++KYMTDG+LLRE++ D  L+ Y VII+DEAHERTL TDIL G+L+E++++RSDL ++V SAT+DA KF  +F  C +  IPGRT PV++ Y      D + AA+   +  H+  + ++GD+L+F++GQE+++  C +I     +V D  P   D+K+ PLYS L  ++Q  IF      K N +  R+ +V+TNIAETS+T+DG+
Sbjct:  133 KRLQLPVWEYKDRFTDILVRHQSFVLVGETGSGKTTQIPQWCVEYMRSLPGPKRGVACTQPRRVAAMSVAQRVADEMDVMLGQEVGYSIRFEDCSSAKTILKYMTDGMLLREAMNDPLLERYGVIILDEAHERTLATDILMGVLKEVVRQRSDLKVIVMSATLDAGKFQIYFDNCPLLTIPGRTHPVEIFYTPEPERDYLEAAIRTVIQIHM-CEEEEGDLLLFLTGQEEIDEACKRIK---REVDDLGPEVGDIKIIPLYSTLPPQQQQRIFEPPPPKKQNGAIGRKVVVSTNIAETSLTIDGV 433          
BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. Ensembl Mouse
Match: Dhx33 (DEAH (Asp-Glu-Ala-His) box polypeptide 33 [Source:MGI Symbol;Acc:MGI:2445102])

HSP 1 Score: 232.261 bits (591), Expect = 7.141e-121
Identity = 126/320 (39.38%), Postives = 192/320 (60.00%), Query Frame = 1
Query: 1960 GMDSLQIYPISQASANQRAGRAGRTGPGICYRLYTINQYENDMLPMTVPEIQRTNLTNVVLLLKSLNVQDLMKFYFMDSPPQDNILNSMYHLWMLGAL---DAVGNLTELGRTMSEFPLDPSLSKMLITSVKHGCSEEMLTIVSMLSVPAIFSRPTGHQEESDCAHEKFQVAESDHLTLLNVYRLWRESGYSANFCKKYYIHNKSMKRVREVRQQLKEIMIKLKMSLASS-GDFDMIRYCICSAYFNQAARWKGLGEYVNLRSGVVCHLHPTSSLYNMAFNADYVVYHELLMTSKEFMNYVTFVDARWLAELGHMFYSVK 2907
            G++ L +  +S+  A QR GRAGR   GICYRLYT +++E     MTVPEIQR NL +V+L L ++ V +++ F FM  P  D+I  ++  L +LGAL   D    LT +GR M+ FPL+P  +K ++ S K  C+EE+LTIVS+LSV ++   P   ++E     +KF  +E DH+TLLN+YR ++  G + ++CK+ ++++K+M  V EVR QL+EI +K+ M + SS GD + +R C+  + F   A  +  G Y    +     +HP+S L++       VVY  LL T+K +M  +  VDA WL E    ++  K
Sbjct:  376 GLEVLAVQRVSKTQAWQRTGRAGREDSGICYRLYTEDEFEK-FEKMTVPEIQRCNLASVILQLLAMKVPNVLTFDFMSKPSPDHIEAAIAQLDLLGALEHKDDQLTLTPIGRKMAAFPLEPRFAKTILLSSKFHCTEEILTIVSLLSVDSVLYNPPARRDEVQSVRKKFISSEGDHITLLNIYRTFKNIGGNKDWCKENFVNSKNMLLVAEVRAQLREICLKMSMPIMSSRGDMESVRRCMAHSLFMNTAELQTDGTYATTDTHQPVAIHPSSVLFHC--KPACVVYTSLLYTNKCYMRDLCVVDAEWLYEAAPDYFRRK 692          

HSP 2 Score: 224.172 bits (570), Expect = 7.141e-121
Identity = 134/309 (43.37%), Postives = 196/309 (63.43%), Query Frame = 3
Query: 1050 SEQKSKIDDQRKRLPIFKVRRDLIRLIRENQVIVIVGETGSGKTTQLTQYLLEENYHHKGIIGCTQPXXXXXXXXXXXXXDEVGTKLGDKVGYSIRFEDCKSENTMIKYMTDGIXXXXXXXXXXXXAYSVIIMDEAHERTLNTDILFGLLREIIQRRSD-----LHLVVTSATMDAEKFSNFFGKCEIFKIPGRTFPVDVIYEKSSILDPVAAAVNKAVDTHLGADSKDGDILVFMSGQEDVEVTCDKIDETLEKVKDTNPPDLKVEPLYSQLSSERQSEIFKINTSGIRRCIVATNIAETSVTLDGIR 1961
            S  +  ++ QR+ LPIF+ R  L+  +R     V++GETGSGKTTQ+ QYL E     +GII  TQPRRVAA+S+A RV+DE  T+LG  VGY++RFED  SE+T IK++TDG+LLRE++ D  L  YS +I+DEAHERT++TD+LFG+++   +RR +     L ++V SATMD + FS +F +  +  + GR  P+ + Y K    D + AA+      H  A +   DILVF++GQE++E       +    + D   P + V PLY+ L   +Q  +F+    G R+ I++TNIAETS+T+ GI+
Sbjct:   52 SPYREALELQRRSLPIFRARGQLLAQLRNLDNAVLIGETGSGKTTQIPQYLYEGGISRQGIIAVTQPRRVAAISLATRVSDEKRTELGKLVGYTVRFEDVTSEDTRIKFLTDGMLLREAISDSLLRKYSCVILDEAHERTIHTDVLFGVVKTAQKRRKELGKLPLKVIVMSATMDVDLFSQYFNRAPVLYLEGRQHPIQIFYTKQPQQDYLHAALVSVFQIHQEAPASQ-DILVFLTGQEEIEAMSKTCRDIARHLPD-GCPSMLVLPLYASLPYSQQLRVFQGAPKGYRKVIISTNIAETSITITGIK 358          
BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. Ensembl Mouse
Match: Dhx15 (DEAH (Asp-Glu-Ala-His) box polypeptide 15 [Source:MGI Symbol;Acc:MGI:1099786])

HSP 1 Score: 240.35 bits (612), Expect = 1.129e-118
Identity = 143/305 (46.89%), Postives = 208/305 (68.20%), Query Frame = 3
Query: 1077 QRKRLPIFKVRRDLIRLIRENQVIVIVGETGSGKTTQLTQYLLEENYHHKGI---IGCTQPXXXXXXXXXXXXXDEVGTKLGDKVGYSIRFEDCKSENTMIKYMTDGIXXXXXXXXXXXXAYSVIIMDEAHERTLNTDILFGLLREIIQRRSDLHLVVTSATMDAEKFSNFFGKCEIFKIPGRTFPVDVIYEKSSILDPVAAAVNKAVDTHLGADSKDGDILVFMSGQEDVEVTCDKIDETLEKVKDTNPP--DLKVEPLYSQLSSERQSEIF------KINTSGIRRCIVATNIAETSVTLDGI 1958
            +R +LP+++ +     ++  +Q  V+VGETGSGKTTQ+ Q+ +E      G    + CTQPRRVAA+SVA+RVADE+   LG +VGYSIRFEDC S  T++KYMTDG+LLRE++ D  L+ Y VII+DEAHERTL TDIL G+L+E++++RSDL ++V SAT+DA KF  +F  C +  IPGRT PV++ Y      D + AA+   +  H+  + ++GD+L+F++GQE+++  C +I     +V D  P   D+K+ PLYS L  ++Q  IF      K N +  R+ +V+TNIAETS+T+DG+
Sbjct:  133 KRLQLPVWEYKDRFTDILVRHQSFVLVGETGSGKTTQIPQWCVEYMRSLPGPKRGVACTQPRRVAAMSVAQRVADEMDVMLGQEVGYSIRFEDCSSAKTILKYMTDGMLLREAMNDPLLERYGVIILDEAHERTLATDILMGVLKEVVRQRSDLKVIVMSATLDAGKFQIYFDNCPLLTIPGRTHPVEIFYTPEPERDYLEAAIRTVIQIHM-CEEEEGDLLLFLTGQEEIDEACKRIK---REVDDLGPEVGDIKIIPLYSTLPPQQQQRIFEPPPPKKQNGAIGRKVVVSTNIAETSLTIDGV 433          

HSP 2 Score: 208.764 bits (530), Expect = 1.129e-118
Identity = 112/251 (44.62%), Postives = 157/251 (62.55%), Query Frame = 1
Query: 1963 MDSLQIYPISQASANQRAGRAGRTGPGICYRLYTINQYENDMLPMTVPEIQRTNLTNVVLLLKSLNVQDLMKFYFMDSPPQDNILNSMYHLWMLGALDAVGNLTELGRTMSEFPLDPSLSKMLITSVKHGCSEEMLTIVSMLSVPAIFSRPTGHQEESDCAHEKFQVAESDHLTLLNVYRLWRESGYSANFCKKYYIHNKSMKRVREVRQQLKEIMIKLKMSLASSGDFDM------IRYCICSAYFNQAA 2697
            ++SL +  IS+ASA QRAGRAGRT PG C+RLYT   Y+ +M   T PEI R+NL +VVL LK L + DL+ F FMD P  + ++ ++  L  L AL+  G+LTELG  M+EFPLDP L+KM+I S  + CS E+L+I +MLSVP  F RPT  ++ +D A  +F   + DHLTLLNVY  ++++  S  +C   +I+ +S+     VRQQL  IM +  +   S+ DF        IR  + + YF Q +
Sbjct:  453 VESLLVTAISKASAQQRAGRAGRTRPGKCFRLYTEKAYKTEMQDNTYPEILRSNLGSVVLQLKKLGIDDLVHFDFMDPPAPETLMRALELLNYLAALNDDGDLTELGSMMAEFPLDPQLAKMVIASCDYNCSNEVLSITAMLSVPQCFVRPTEAKKAADEAKMRFAHIDGDHLTLLNVYHAFKQNHESVQWCYDNFINYRSLMSADNVRQQLSRIMDRFNLPRRST-DFTSRDYYINIRKALVTGYFMQVS 702          
BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. UniProt/SwissProt
Match: sp|Q92620|PRP16_HUMAN (Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 OS=Homo sapiens OX=9606 GN=DHX38 PE=1 SV=2)

HSP 1 Score: 500.745 bits (1288), Expect = 0.000e+0
Identity = 296/603 (49.09%), Postives = 404/603 (67.00%), Query Frame = 3
Query:  243 RHKQWEEMTPSTSLRDETGRYSEIRTPMIDRSIK----------TPSV--TSWDEDDVTFKRPDTVTSFRRN----------DKSEIKWSPDKNEEELDREWYLND-GME--YNPFPNFSDEVLEKKKQQLQRQTSIVAKSRKSARAKQFEETNRAWEESKLINSGAVVRKGFNVEIDNEDTGEDRIHLHVHNILPPFLDGRIVFTKQFEPVIPVKDENSDMAKIAKNGSQMVKNYRDIKEKMKLQKRTLDMTGTNFGKLMGLEKKETLDKW----DETNYKDSMKYCDKITDKIALDSE--QKSKIDDQRKRLPIFKVRRDLIRLIRENQVIVIVGETGSGKTTQLTQYLLEENYHHKGIIGCTQPXXXXXXXXXXXXXDEVGTKLGDKVGYSIRFEDCKSENTMIKYMTDGIXXXXXXXXXXXXAYSVIIMDEAHERTLNTDILFGLLREIIQRRSDLHLVVTSATMDAEKFSNFFGKCEIFKIPGRTFPVDVIYEKSSILDPVAAAVNKAVDTHLGADSKDGDILVFMSGQEDVEVTCDKIDETLEKVKDTNPPDLKVEPLYSQLSSERQSEIFKINTSGIRRCIVATNIAETSVTLDGI 1958
            R  QWE  +P+ S RD + R   + T   DRS++          TPS     W +D         ++  R            D  E +   + ++ + DR+WY+ D G +  +NP    S++ + +++Q L +Q     + R SA+ +Q  E N  WE ++++ SG V R    V+ D E+    ++HL VHN++PPFLDGRIVFTKQ EPVIPVKD  SD+A IA+ GSQ V+ +R+ KE+ K Q +  ++ GT  G +MG++K+E  DK      + +Y+   K+ D +  K    SE  +K  I +QR+ LPIF V+++L+ +IR+N ++++VGETGSGKTTQLTQYL E+ Y   G+IGCTQPRRVAA+SVAKRV++E+G  LG++VGY+IRFEDC SENT+IKYMTDGILLRESLR+ DLD YS IIMDEAHER+LNTD+LFGLLRE++ RRSDL L+VTSATMDAEKF+ FFG   IF IPGRTFPVD+++ K+   D V AAV +++  HL      GDIL+FM GQED+EVT D+I E LE+++  N P L V P+YSQL S+ Q++IF+    G+R+CIVATNIAETS+T+DGI
Sbjct:  226 RRSQWESPSPTPSYRD-SERSHRLSTRDRDRSVRGKYSDDTPLPTPSYKYNEWADDRRHLGSTPRLSRGRGRREEGEEGISFDTEEERQQWEDDQRQADRDWYMMDEGYDEFHNPLAYSSEDYVRRREQHLHKQK----QKRISAQRRQINEDNERWETNRMLTSGVVHR--LEVDEDFEEDNAAKVHLMVHNLVPPFLDGRIVFTKQPEPVIPVKDATSDLAIIARKGSQTVRKHREQKERKKAQHKHWELAGTKLGDIMGVKKEEEPDKAVTEDGKVDYRTEQKFADHMKRKSEASSEFAKKKSILEQRQYLPIFAVQQELLTIIRDNSIVIVVGETGSGKTTQLTQYLHEDGYTDYGMIGCTQPRRVAAMSVAKRVSEEMGGNLGEEVGYAIRFEDCTSENTLIKYMTDGILLRESLREADLDHYSAIIMDEAHERSLNTDVLFGLLREVVARRSDLKLIVTSATMDAEKFAAFFGNVPIFHIPGRTFPVDILFSKTPQEDYVEAAVKQSLQVHLSG--APGDILIFMPGQEDIEVTSDQIVEHLEELE--NAPALAVLPIYSQLPSDLQAKIFQKAPDGVRKCIVATNIAETSLTVDGI 817          

HSP 2 Score: 474.167 bits (1219), Expect = 0.000e+0
Identity = 217/322 (67.39%), Postives = 264/322 (81.99%), Query Frame = 1
Query: 1960 GMDSLQIYPISQASANQRAGRAGRTGPGICYRLYTINQYENDMLPMTVPEIQRTNLTNVVLLLKSLNVQDLMKFYFMDSPPQDNILNSMYHLWMLGALDAVGNLTELGRTMSEFPLDPSLSKMLITSVKHGCSEEMLTIVSMLSVPAIFSRPTGHQEESDCAHEKFQVAESDHLTLLNVYRLWRESGYSANFCKKYYIHNKSMKRVREVRQQLKEIMIKLKMSLASSG-DFDMIRYCICSAYFNQAARWKGLGEYVNLRSGVVCHLHPTSSLYNMAFNADYVVYHELLMTSKEFMNYVTFVDARWLAELGHMFYSVKEKGQS 2922
            GMD+LQIYPISQA+ANQR+GRAGRTGPG C+RLYT + Y+N++L  TVPEIQRTNL NVVLLLKSL VQDL++F+FMD PP+DN+LNSMY LW+LGALD  G LT  GR M EFPLDP+LSKMLI S   GCS E+L IVSMLSVPAIF RP G +EESD   EKF V ESDHLT LNVY  W+ + YS  +C  ++IH K+M++VREVR QLK+IM++ +MSLAS G D+D++R CIC+AYF+QAA+ KG+GEYVN+R+G+ CHLHPTSSL+ M +  DY+VYHEL+MT+KE+M  VT VD  WLAELG MFYSVK+ G+S
Sbjct:  836 GMDALQIYPISQANANQRSGRAGRTGPGQCFRLYTQSAYKNELLTTTVPEIQRTNLANVVLLLKSLGVQDLLQFHFMDPPPEDNMLNSMYQLWILGALDNTGGLTSTGRLMVEFPLDPALSKMLIVSCDMGCSSEILLIVSMLSVPAIFYRPKGREEESDQIREKFAVPESDHLTYLNVYLQWKNNNYSTIWCNDHFIHAKAMRKVREVRAQLKDIMVQQRMSLASCGTDWDIVRKCICAAYFHQAAKLKGIGEYVNIRTGMPCHLHPTSSLFGMGYTPDYIVYHELVMTTKEYMQCVTAVDGEWLAELGPMFYSVKQAGKS 1157          
BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. UniProt/SwissProt
Match: sp|Q17R09|PRP16_BOVIN (Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 OS=Bos taurus OX=9913 GN=DHX38 PE=2 SV=1)

HSP 1 Score: 499.975 bits (1286), Expect = 0.000e+0
Identity = 296/602 (49.17%), Postives = 400/602 (66.45%), Query Frame = 3
Query:  243 RHKQWEEMTPSTSLRDETGRYSEIRTPMI---------DRSIKTPSV--TSWDEDDVTFKRPDTVTSFR-RNDKSEIKWSPDKNEE---------ELDREWYLND-GME--YNPFPNFSDEVLEKKKQQLQRQTSIVAKSRKSARAKQFEETNRAWEESKLINSGAVVRKGFNVEIDNEDTGEDRIHLHVHNILPPFLDGRIVFTKQFEPVIPVKDENSDMAKIAKNGSQMVKNYRDIKEKMKLQKRTLDMTGTNFGKLMGLEKKETLDKW----DETNYKDSMKYCDKITDKIALDSE--QKSKIDDQRKRLPIFKVRRDLIRLIRENQVIVIVGETGSGKTTQLTQYLLEENYHHKGIIGCTQPXXXXXXXXXXXXXDEVGTKLGDKVGYSIRFEDCKSENTMIKYMTDGIXXXXXXXXXXXXAYSVIIMDEAHERTLNTDILFGLLREIIQRRSDLHLVVTSATMDAEKFSNFFGKCEIFKIPGRTFPVDVIYEKSSILDPVAAAVNKAVDTHLGADSKDGDILVFMSGQEDVEVTCDKIDETLEKVKDTNPPDLKVEPLYSQLSSERQSEIFKINTSGIRRCIVATNIAETSVTLDGI 1958
            R  QWE  +P+ S RD    +                 D  + TPS     W +D         ++  R R +  E   S D  EE         + DR+WY+ D G +  +NP    SD+ + +++Q L +Q     + R SA+ +Q  E N  WE ++++ SG V R    V+ D E+    ++HL VHN++PPFLDGRIVFTKQ EPVIPVKD  SD+A IA+ GSQ V+ +R+ KE+ K Q +  ++ GT  G +MG++K+E  DK      + +Y+   K+ D +  K    SE  +K  I +QR+ LPIF V+++L+ +IR+N ++++VGETGSGKTTQLTQYL E+ Y   G+IGCTQPRRVAA+SVAKRV++E+G  LG++VGY+IRFEDC SE+T+IKYMTDGILLRESLR+ DLD YS IIMDEAHER+LNTD+LFGLLRE++ RRSDL L+VTSATMDAEKF++FFG   IF IPGRTFPVD+++ K+   D V AAV +++  HL      GDIL+FM GQED+EVT D+I E LE+++  N P L V P+YSQL S+ Q++IF+    G+R+CIVATNIAETS+T+DGI
Sbjct:  226 RRSQWESPSPTPSYRDSERSHRPSSRDRDRSVRSRYSDDTPLPTPSYKYNEWADDRRHLGSTPRLSRGRGRREDGEEGISFDTEEERQQWEDDQRQADRDWYMMDEGYDEFHNPLAYSSDDYVRRREQHLHKQK----QKRISAQRRQINEDNERWETNRMLTSGVVHR--LEVDEDFEEDSAAKVHLMVHNLVPPFLDGRIVFTKQPEPVIPVKDATSDLAIIARKGSQTVRKHREQKERKKAQHKHWELAGTKLGDIMGVKKEEEPDKSLTEDGKVDYRTEQKFADHMKKKSEASSEFAKKKSILEQRQYLPIFAVQQELLTIIRDNSIVIVVGETGSGKTTQLTQYLHEDGYTDYGMIGCTQPRRVAAMSVAKRVSEEMGGNLGEEVGYAIRFEDCTSESTLIKYMTDGILLRESLREADLDHYSAIIMDEAHERSLNTDVLFGLLREVVARRSDLKLIVTSATMDAEKFASFFGNVPIFHIPGRTFPVDILFSKTPQEDYVEAAVKQSLQVHLSG--APGDILIFMPGQEDIEVTSDQIVEHLEELE--NAPALAVLPIYSQLPSDLQAKIFQKAPDGVRKCIVATNIAETSLTVDGI 817          

HSP 2 Score: 473.396 bits (1217), Expect = 0.000e+0
Identity = 216/322 (67.08%), Postives = 264/322 (81.99%), Query Frame = 1
Query: 1960 GMDSLQIYPISQASANQRAGRAGRTGPGICYRLYTINQYENDMLPMTVPEIQRTNLTNVVLLLKSLNVQDLMKFYFMDSPPQDNILNSMYHLWMLGALDAVGNLTELGRTMSEFPLDPSLSKMLITSVKHGCSEEMLTIVSMLSVPAIFSRPTGHQEESDCAHEKFQVAESDHLTLLNVYRLWRESGYSANFCKKYYIHNKSMKRVREVRQQLKEIMIKLKMSLASSG-DFDMIRYCICSAYFNQAARWKGLGEYVNLRSGVVCHLHPTSSLYNMAFNADYVVYHELLMTSKEFMNYVTFVDARWLAELGHMFYSVKEKGQS 2922
            GMD+LQIYPISQA+ANQR+GRAGRTGPG C+RLYT + Y+N++L  TVPEIQRTNL NVVLLLKSL VQDL++F+FMD PP+DN+LNSMY LW+LGALD  G LT  GR M EFPLDP+LSKMLI S   GCS E+L IVSMLSVPAIF RP G +EESD   EKF V ESDHL+ LNVY  W+ + YS  +C  ++IH K+M++VREVR QLK+IM++ +MSLAS G D+D++R CIC+AYF+QAA+ KG+GEYVN+R+G+ CHLHPTSSL+ M +  DY+VYHEL+MT+KE+M  VT VD  WLAELG MFYSVK+ G+S
Sbjct:  836 GMDALQIYPISQANANQRSGRAGRTGPGQCFRLYTQSAYKNELLTTTVPEIQRTNLANVVLLLKSLGVQDLLQFHFMDPPPEDNMLNSMYQLWILGALDNTGGLTSTGRLMVEFPLDPALSKMLIVSCDMGCSSEILLIVSMLSVPAIFYRPKGREEESDQIREKFAVPESDHLSYLNVYLQWKNNNYSTIWCNDHFIHAKAMRKVREVRAQLKDIMVQQRMSLASCGTDWDIVRKCICAAYFHQAAKLKGIGEYVNIRTGMPCHLHPTSSLFGMGYTPDYIVYHELVMTTKEYMQCVTAVDGEWLAELGPMFYSVKQAGKS 1157          
BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. UniProt/SwissProt
Match: sp|P34498|MOG1_CAEEL (Probable pre-mRNA-splicing factor ATP-dependent RNA helicase mog-1 OS=Caenorhabditis elegans OX=6239 GN=mog-1 PE=1 SV=2)

HSP 1 Score: 491.5 bits (1264), Expect = 0.000e+0
Identity = 238/379 (62.80%), Postives = 287/379 (75.73%), Query Frame = 1
Query: 1960 GMDSLQIYPISQASANQRAGRAGRTGPGICYRLYTINQYENDMLPMTVPEIQRTNLTNVVLLLKSLNVQDLMKFYFMDSPPQDNILNSMYHLWMLGALDAVGNLTELGRTMSEFPLDPSLSKMLITSVKHGCSEEMLTIVSMLSVPAIFSRPTGHQEESDCAHEKFQVAESDHLTLLNVYRLWRESGYSANFCKKYYIHNKSMKRVREVRQQLKEIMIKLKMSLASSG-DFDMIRYCICSAYFNQAARWKGLGEYVNLRSGVVCHLHPTSSLYNMAFNADYVVYHELLMTSKEFMNYVTFVDARWLAELGHMFYSVKEKGQSVTDRMLKEQQTVERMETEMRINEKANLKKLLDNRVIESERRAQSVKETTYSRPRSSR 3093
            GMD+L I+P+SQASANQR GRAGRTGPG CYRLYT  Q+++++L  TVPEIQRTNL NVVLLLKSL V DL+KF+FMD+PPQDN+LNSMY LW LGALD  G LT +GR M EFPLDP+LSKMLI S + GCS+E+LTIVSMLSVPAIF RP G +EE+D   EKFQV ESDHLT LNVY  WR   YSA +C   Y+H K++K+VREVR QLKEIM  LK+ L S+G ++D++R CICSAYF+ AAR KG+GEYVN+R+G+ C LHPTS+L+ M F  DYVVYHEL+MT+KE+M  VT VDA WLAELG MFYS+KE  QS  +  ++  +TVE ME EMR  +K            E ERR +   +  + RP SSR
Sbjct:  746 GMDALSIFPVSQASANQRTGRAGRTGPGQCYRLYTERQFKDELLKSTVPEIQRTNLANVVLLLKSLGVDDLLKFHFMDAPPQDNMLNSMYQLWTLGALDNTGQLTPMGRKMVEFPLDPTLSKMLIMSAEMGCSDEVLTIVSMLSVPAIFFRPKGREEEADAKKEKFQVPESDHLTFLNVYIQWRTHKYSAKWCADNYLHVKALKKVREVRAQLKEIMQDLKLPLISNGSEWDIVRKCICSAYFHNAARLKGIGEYVNVRTGIPCFLHPTSALFGMGFMPDYVVYHELIMTAKEYMQCVTAVDAIWLAELGPMFYSIKESKQSRKELKMESVRTVETMEAEMREAQK------------EMERRKEE-SDKAFKRPESSR 1111          

HSP 2 Score: 480.715 bits (1236), Expect = 0.000e+0
Identity = 272/522 (52.11%), Postives = 365/522 (69.92%), Query Frame = 3
Query:  420 NDKSEIKWSPDKNEEELDREWYLNDGM---EYNPFPNFSDEVLEKKKQQLQRQTSIVAKSRKSARAKQFEETNRAWEESKLINSGAVVRKGFNVEIDNEDTGEDRIHLHVHNILPPFLDGRIVFTKQFEPVIPVKDENSDMAKIAKNGSQMVKNYRDIKEKMKLQKRTLDMTGTNFGKLMGLEKK--ETLDKWDE--TNYKDSMKYCDKITDKIAL-DSEQKSKIDDQRKRLPIFKVRRDLIRLIRENQVIVIVGETGSGKTTQLTQYLLEENYHHKGIIGCTQPXXXXXXXXXXXXXDEVGTKLGDKVGYSIRFEDCKSENTMIKYMTDGIXXXXXXXXXXXXAYSVIIMDEAHERTLNTDILFGLLREIIQRRSDLHLVVTSATMDAEKFSNFF-GKCEIFKIPGRTFPVDVIYEKSSILDPVAAAVNKAVDTHLGADSKDGDILVFMSGQEDVEVTCDKIDETLEKVKDTNPPDLKVEPLYSQLSSERQSEIFKINTSGIRRCIVATNIAETSVTLDGI 1958
            +D+   +W  ++ ++ LDREWY N+G    EYNPF   SDE +EK+++Q Q +T    K R + + +  +  N  WE ++L  SG V        I  ++T E+R+ + V NI+PPFLDGRIVFTKQ +P+IPV D   DMA  A  GS  V+  R+++++ K Q +  ++ G+  G LMG+++K  ET D  D+   NYK+S ++   + D  A+ D   +  I  QR+ LP+F  R+ ++ +IREN V++IVGETGSGKTTQL QYLLE+ +   G+IGCTQPRRVAA+SVA+RVADE+G  LG  VGY+IRFEDC SE T+IKYMTDGILLRE L D  LD YS IIMDEAHER+LNTD+LFGLLRE+I +R+DL L+VTSATMDA+KF++FF G C  F IPGRTFPV++ + ++ + D V AAV +AV  HLG    DGDIL+FM GQED+E TC+ I E L ++ D  PP L V P+YSQL S+ Q++IF+    G+R+ IVATNIAETS+T+DGI
Sbjct:  215 DDEERAQW--EEEQKNLDREWYDNEGAFDDEYNPFNKVSDEFVEKREKQWQEKTQ---KPRLTVKQQAIKRENELWENNRLHRSGVVAMADELSSIFEDETDENRVTILVQNIVPPFLDGRIVFTKQAQPIIPVVDTTCDMAVSAARGSVAVRKRREVEDRKKAQDKHWELAGSKLGNLMGVKEKKDETADPEDDDSGNYKESHQFASHMKDNEAVSDFAMEKSIKQQREYLPVFACRQKMMNVIRENNVVIIVGETGSGKTTQLAQYLLEDGFGDSGLIGCTQPRRVAAMSVARRVADEMGVDLGQDVGYAIRFEDCTSEKTIIKYMTDGILLRECLGDGSLDQYSAIIMDEAHERSLNTDVLFGLLREVIAKRADLKLIVTSATMDADKFADFFGGNCPTFTIPGRTFPVELFHARTPVEDYVDAAVKQAVTIHLGG--MDGDILIFMPGQEDIECTCEMIKEKLGEL-DEAPP-LAVLPIYSQLPSDLQAKIFQRAPGGMRKAIVATNIAETSLTVDGI 727          
BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. UniProt/SwissProt
Match: sp|F4K2E9|PRP16_ARATH (Pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH7 OS=Arabidopsis thaliana OX=3702 GN=CUV PE=1 SV=1)

HSP 1 Score: 433.335 bits (1113), Expect = 0.000e+0
Identity = 208/385 (54.03%), Postives = 280/385 (72.73%), Query Frame = 1
Query: 1960 GMDSLQIYPISQASANQRAGRAGRTGPGICYRLYTINQYENDMLPMTVPEIQRTNLTNVVLLLKSLNVQDLMKFYFMDSPPQDNILNSMYHLWMLGALDAVGNLTELGRTMSEFPLDPSLSKMLITSVKHGCSEEMLTIVSMLSVPAIFSRPTGHQEESDCAHEKFQVAESDHLTLLNVYRLWRESGYSANFCKKYYIHNKSMKRVREVRQQLKEIMIKLKMSLASSG-DFDMIRYCICSAYFNQAARWKGLGEYVNLRSGVVCHLHPTSSLYNMAFNADYVVYHELLMTSKEFMNYVTFVDARWLAELGHMFYSVKEKGQSVTDRMLKEQQTVERMETEMRINEKANLKKLLDNRVIESERRAQSVKE---------TTYSRPR 3084
            GMD+LQ++PIS+A+++QRAGRAGRTGPG CYRLYT + Y N+MLP  VPEIQRTNL NVVLLLKSL + +L+ F FMD PPQ+NILNSMY LW+LGAL+ VG LT+LG  M EFPLDP L+KML+   +  C +E+LTIVSMLSVP++F RP    EESD A EKF V ESDHLTLLNVY+ W+E  Y  ++C  +Y+  K +++ REVR QL +I+ +LK+ L S G D+D++R  ICSAYF+ +AR KG+GEYVN R+G+ CHLHP+S+LY + +  DYVVYHEL++T+KE+M   T V+  WLAELG MF+SVK+   S+ +   K+++    ME EM    +  ++  L ++  E ++RA+  ++         TT+ RP+
Sbjct:  867 GMDALQVFPISRAASDQRAGRAGRTGPGTCYRLYTESAYLNEMLPSPVPEIQRTNLGNVVLLLKSLKIDNLLDFDFMDPPPQENILNSMYQLWVLGALNNVGGLTDLGWKMVEFPLDPPLAKMLLMGERLDCIDEVLTIVSMLSVPSVFFRPKERAEESDAAREKFFVPESDHLTLLNVYQQWKEHDYRGDWCNDHYLQVKGLRKAREVRSQLLDILKQLKIELRSCGPDWDIVRKAICSAYFHNSARLKGVGEYVNCRTGMPCHLHPSSALYGLGYTPDYVVYHELILTTKEYMQCATSVEPHWLAELGPMFFSVKDSDTSMLEHKKKQKEEKSGMEEEMEKLRRDQVESELRSKERERKKRAKQQQQISGPGLKKGTTFLRPK 1251          

HSP 2 Score: 425.631 bits (1093), Expect = 0.000e+0
Identity = 250/522 (47.89%), Postives = 354/522 (67.82%), Query Frame = 3
Query:  459 EEELDREWYLND------GMEYNPFPNFSDEVLEKKKQQLQRQTSIVAKSRKS-ARAKQFEETNR---AWEESKLINSGAVVRKGFNVEIDNEDTGEDRIHLHVHNILPPFLDGRIVFTKQFEPVIPVKDENSDMAKIAKNGSQMVKNYRDIKEKMKLQKRTLDMTGTNFGKLMGLEKKETLDKWD--------ETNYKDSMKYCDKITDKIALDSEQKSK-IDDQRKRLPIFKVRRDLIRLIRENQVIVIVGETGSGKTTQLTQYLLEENYHHKGIIGCTQPXXXXXXXXXXXXXDEVGTKLGDKVGYSIRFEDCKSENTMIKYMTDGIXXXXXXXXXXXXAYSVIIMDEAHERTLNTDILFGLLREIIQRRSDLHLVVTSATMDAEKFSNFFGKCEIFKIPGRTFPVDVIYEKSSILDPVAAAVNKAVDTHLGADSKDGDILVFMSGQEDVEVTCDKIDETLEKVKDTNP---PDLKVEPLYSQLSSERQSEIFKINTSGIRRCIVATNIAETSVTLDGI 1958
            E + DR WY  D        +   F    D  L+KK+ +L ++      S+ S A++K++ + N     WE+ +L+ SGAV       E D+E+  E +  L VH+  PPFLDGR+V+TKQ EPV+PVKD  SDMA I++ GS +VK  R+ +   K ++R  ++ G+N G ++G+EK       D        E ++K   K+   +    A+     SK + +QR+ LPIF VR +L+++IRENQVIV+VGETGSGKTTQLTQYL E+ Y   GI+GCTQPRRVAA+SVAKRV++E+ T+LGDK+GY+IRFED    NT+IKYMTDG+LLRE+L+D DLD Y V++MDEAHER+LNTD+LFG+L++++ RR D  L+VTSAT++A+KFSNFFG   IF IPGRTFPV+++Y K+   D V AAV +A+  H+   S  GDIL+FM+GQ+++E  C  + E +E++  ++     +L + P+YSQL ++ Q++IF+    G R+CIVATNIAETS+T+DGI
Sbjct:  331 EYQSDRAWYDTDEGNSLFDADSASFFLGDDASLQKKETELAKRLVRRDGSKMSLAQSKKYSQLNADNAQWEDRQLLRSGAVRGTEVQTEFDSEE--ERKAILLVHDTKPPFLDGRVVYTKQAEPVMPVKDPTSDMAIISRKGSGLVKEIREKQSANKSRQRFWELAGSNLGNILGIEKSAEQIDADTAVVGDDGEVDFKGEAKFAQHMKKGEAVSEFAMSKTMAEQRQYLPIFSVRDELLQVIRENQVIVVVGETGSGKTTQLTQYLHEDGYTINGIVGCTQPRRVAAMSVAKRVSEEMETELGDKIGYAIRFEDVTGPNTVIKYMTDGVLLRETLKDSDLDKYRVVVMDEAHERSLNTDVLFGILKKVVARRRDFKLIVTSATLNAQKFSNFFGSVPIFNIPGRTFPVNILYSKTPCEDYVEAAVKQAMTIHI--TSPPGDILIFMTGQDEIEAACFSLKERMEQLVSSSSREITNLLILPIYSQLPADLQAKIFQKPEDGARKCIVATNIAETSLTVDGI 848          
BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. UniProt/SwissProt
Match: sp|Q9P774|PRP16_SCHPO (Pre-mRNA-splicing factor ATP-dependent RNA helicase prp16 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=prp16 PE=3 SV=2)

HSP 1 Score: 424.861 bits (1091), Expect = 0.000e+0
Identity = 201/367 (54.77%), Postives = 275/367 (74.93%), Query Frame = 1
Query: 1960 GMDSLQIYPISQASANQRAGRAGRTGPGICYRLYTINQYENDMLPMTVPEIQRTNLTNVVLLLKSLNVQDLMKFYFMDSPPQDNILNSMYHLWMLGALDAVGNLTELGRTMSEFPLDPSLSKMLITSVKHGCSEEMLTIVSMLSVPAIFSRPTGHQEESDCAHEKFQVAESDHLTLLNVYRLWRESGYSANFCKKYYIHNKSMKRVREVRQQLKEIMIKLKMSLASSGDFDMIRYCICSAYFNQAARWKGLGEYVNLRSGVVCHLHPTSSLYNMAFNADYVVYHELLMTSKEFMNYVTFVDARWLAELGHMFYSVKEKGQSVT---DRMLKEQQTVE-RMETEMRINEKANLKKLLDNRVIESERRA 3048
            G+D+LQ+ PISQA+ANQRAGRAGRTGPGI YRLYT   Y  +M   T+PEIQRTNL+N VL+LKSL V+++  F FMD PP D ++ S+Y LW LGALD  G LT LG+ MS FP+DPSLSK++I +  + C+EE++TIVSMLSVP++F RP    EESD A EKF V ESDHL LLN+Y+ W+ +GYS ++C K+++H+K++KR R++RQQL EIM K K+SL S  D+D++R  +CSAYF+QAA  KG+GEYV+LRSG+ CHLH TSSLY + +  DYV+YHEL++TSKE+MN VT VD  WLAE G ++YSVKE+ ++ T   DR+   +  ++ ++  +  ++ K  L K  +   ++S+R++
Sbjct:  796 GIDTLQVTPISQANANQRAGRAGRTGPGIAYRLYTEMAYIREMFETTLPEIQRTNLSNTVLILKSLGVEEISDFDFMDRPPNDTLMASLYELWTLGALDNFGKLTTLGKKMSLFPMDPSLSKLIIIAEDYKCTEEIITIVSMLSVPSVFYRPKERAEESDAAREKFNVPESDHLMLLNIYQHWQRNGYSNSWCSKHFLHSKTLKRARDIRQQLVEIMSKQKISLESVSDWDIVRRVLCSAYFHQAACAKGIGEYVHLRSGMPCHLHVTSSLYGLGYLPDYVIYHELVLTSKEYMNIVTSVDPYWLAEFGGVYYSVKERFRNETESYDRVFSSKPQLDAQIAADRELDAKQKLAK--NQEPVKSKRKS 1160          

HSP 2 Score: 394.43 bits (1012), Expect = 0.000e+0
Identity = 247/559 (44.19%), Postives = 351/559 (62.79%), Query Frame = 3
Query:  357 TSWDEDDVTFKRPDTVTSFRRNDKSEIKWSPDKNEEELDREWYLNDGME-------YNPFPNFSD--------EVLEKKKQQLQRQTSIVAKSRKSARAKQFEETNRAWEESKLINSGAVVRKGFNVEIDNEDTGEDRIHLHVHNILPPFLDGRIVFTKQFEPVIPVKDENSDMAKIAKNGSQMVKNYRDIKEKMKLQKRTLDMTGTNFGKLMGLEKKETLDKWDETNYKDSMKYCDKITDKIALDSEQKSKID----------DQRKRLPIFKVRRDLIRLIRENQVIVIVGETGSGKTTQLTQYLLEENYHHKGIIGCTQPXXXXXXXXXXXXXDEVGTKLGDKVGYSIRFEDCKSENTMIKYMTDGIXXXXXXXXXXXXAYSVIIMDEAHERTLNTDILFGLLREIIQRRSDLHLVVTSATMDAEKFSNFFGKCEIFKIPGRTFPVDVIYEKSSILDPVAAAVNKAVDTHLGADSKDGDILVFMSGQEDVEVTCDKIDETLEKVKDTNPPDLKVEPLYSQLSSERQSEIFKINTSGIRRCIVATNIAETSVTLDGI 1958
            +SWD  DV +   D  +   R      +W  ++ +  LDR+WY+N   +       +NPF +F          E +EK+K+ L    SI A  R       F+E N  WE++++I SG  V K   +E D     E R+HL V  + P FLDG    +K+   +  V+D  SD+A  A+ GS++V+  R+ +E+ K       + GT+ G +MGL+         ++N +D+      +  K+A  SEQ +K D          +QR+ LP F VR  L+ +IR+NQV+++VGETGSGKTTQL Q+L E+ YH  G+IGCTQPRRVAA+SVAKRV++E+G +LG  VGYSIRFED    +T+IKYMTDG+LLRESL   +L+ YSVIIMDEAHER+LNTDIL GLL++++ RR D+ L+VTSATM+++KFS+FFG    F IPGRT+PVD+++ K+   D V AAV + +  HL   +  GDILVFM+GQED+E TC+ I + L ++ D   P L + P+YSQ+ ++ Q++IF     G+R+ +VATNIAETS+T+ GI
Sbjct:  255 SSWDARDVEYPEEDPESKADRQ-----RW--EEEQAHLDRDWYMNSESQNLLGDEVHNPFSDFETVEDRAHEAEFIEKQKKHL----SIEASDR-------FKE-NSMWEKNRMITSG--VSKAPGLESDYSLMEERRVHLLVDELRPHFLDGAEFSSKKVGDITSVRDPQSDLAINARLGSRLVRERREFRERQKAASAATSLAGTSLGNVMGLK---------DSNDEDAKAGTTPV--KVAGRSEQSNKKDTEFARTKSYREQREFLPAFAVREQLLSVIRDNQVLIVVGETGSGKTTQLAQFLYEDGYHRNGMIGCTQPRRVAAMSVAKRVSEEMGVRLGSTVGYSIRFEDVTGPDTVIKYMTDGVLLRESLMQNNLEKYSVIIMDEAHERSLNTDILMGLLKKVLSRRRDIKLLVTSATMNSQKFSDFFGGAPQFTIPGRTYPVDIMFAKAPCSDYVEAAVRQVLQIHLSQPA--GDILVFMTGQEDIEATCEIIADRLNQLHDA--PRLSILPIYSQMPADLQAKIFDSAEPGVRKVVVATNIAETSLTVHGI 777          
BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. TrEMBL
Match: A0A0R3UJK8 (Uncharacterized protein OS=Mesocestoides corti OX=53468 GN=MCOS_LOCUS7702 PE=4 SV=1)

HSP 1 Score: 518.079 bits (1333), Expect = 0.000e+0
Identity = 321/663 (48.42%), Postives = 435/663 (65.61%), Query Frame = 3
Query:  129 DNEEYIKRQNERKQRDEKRKIGESFGSKSKIKKIENIDRHKQWEEMTP-----STSLRDETGRYSE-IRTPMIDRSIKTPSVT---SWDEDDVTFKR-------PDTVTSFRRND-----------------KSEIKWSP------DKNEEELDREWYL-NDGME--YNPFPNFSDEVLEKKKQQLQRQTSIVAKSRKSARAKQFEETNRAWEESKLINSGAVVRKG----FNVEIDNEDTGEDRIHLHVHNILPPFLDGRIVFTKQFEPVIPVKDENSDMAKIAKNGSQMVKNYRDIKEKMKLQKRTLDMTGTNFGKLMGLEKK--ETLDKWDETNYKDSMKYCDKITDK----IALDSEQKSKIDDQRKRLPIFKVRRDLIRLIRENQVIVIVGETGSGKTTQLTQYLLEENYHHKGIIGCTQPXXXXXXXXXXXXXDEVGTKLGDKVGYSIRFEDCKSENTMIKYMTDGIXXXXXXXXXXXXAYSVIIMDEAHERTLNTDILFGLLREIIQRRSDLHLVVTSATMDAEKFSNFFGKCEIFKIPGRTFPVDVIYEKSSILDPVAAAVNKAVDTHLGADSKDGDILVFMSGQEDVEVTCDKIDETLEKVKDTNPPDLKVEPLYSQLSSERQSEIFKINTSGIRRCIVATNIAETSVTLDGIR 1961
            D+ ++ + Q+ R +R   R      GS+   ++I    R + WE  TP     S      +  YS  I TP   R   TP+ +   +W+ DD++ K+       P    SF  N                   ++  ++P      +   E LDR WYL +DG +   NPF +  DE + K+++QL        K R SARA Q ++ N+AWE ++++ SG V R         E D ++ GE RIHL V NI+PPFLDGRI+FTK  EPV+PVKD +  M K+A  GS MV+ +R+ KE+ + QK+  ++ GT  G++MG+++   E  DKW E +Y++S  + DK+ D+    ++  ++QK+ I +QR+ LPIF VR  L+RLI+E+Q++VIVGETGSGKTTQLTQYL EE + + G++GCTQPRRVAA+SVAKRVADE+G  LGD+VGY IRFEDC +E T+IKYMTDGILLRESLR+ DLD YS IIMDEAHER+LNTD+LFGLLREI+ RR+DL L++TSATMD+EKF+ FFG C IF IPGRTFPVD+ + K+ ++D V AAV +A+  HLGA   +GDILVFM+GQED+EVTC+ I E L  + D  PP L V P+YSQL S+ Q++IF+     IR+C+VATNIAETS+T+DGIR
Sbjct:  132 DDRDHHRHQSGRNERASSR-----IGSEESARRI----RERDWEAETPRSGDSSHRSHGTSSSYSRYIETPRHLRPSDTPTPSRSAAWENDDLSEKKRTRYDYTPMQTPSFMHNSWMKGGGRGKQVAPTPATGNQTPFTPVDPQEMEAEAERLDRNWYLMDDGYDEGNNPFIDVPDEYVAKRERQLAEARK--RKIRMSARAVQIQKENQAWEINRMLRSGVVERIAGAEGGLDEEDLDEEGEARIHLLVKNIMPPFLDGRIIFTKHPEPVLPVKDPDGVMTKVAAKGSAMVRYFREQKERRRAQKKEWELAGTRIGEIMGIKQADNEEGDKWHEGSYRESQTFADKMVDQKDQAVSAFAKQKT-IKEQRQYLPIFGVRSALLRLIKEHQIVVIVGETGSGKTTQLTQYLHEEGFTNYGMVGCTQPRRVAAMSVAKRVADEMGVCLGDEVGYVIRFEDCTNEKTVIKYMTDGILLRESLRESDLDPYSAIIMDEAHERSLNTDVLFGLLREIVSRRNDLKLIITSATMDSEKFAQFFGDCPIFHIPGRTFPVDIQFSKTPVMDYVDAAVKQAIQVHLGA--PEGDILVFMTGQEDIEVTCELIAERLGSL-DEAPP-LSVLPIYSQLPSDLQAKIFQKTEDNIRKCVVATNIAETSLTVDGIR 778          

HSP 2 Score: 426.402 bits (1095), Expect = 0.000e+0
Identity = 202/322 (62.73%), Postives = 248/322 (77.02%), Query Frame = 1
Query: 1960 GMDSLQIYPISQASANQRAGRAGRTGPGICYRLYTINQYENDMLPMTVPEIQRTNLTNVVLLLKSLNVQDLMKFYFMDSPPQDNILNSMYHLWMLGALDAVGNLTELGRTMSEFPLDPSLSKMLITSVKHGCSEEMLTIVSMLSVPAIFSRPTGHQEESDCAHEKFQVAESDHLTLLNVYRLWRESGYSANFCKKYYIHNKSMKRVREVRQQLKEIMIKLKMSLASSG-DFDMIRYCICSAYFNQAARWKGLGEYVNLRSGVVCHLHPTSSLYNMAFNADYVVYHELLMTSKEFMNYVTFVDARWLAELGHMFYSVKEKGQS 2922
            GMD+LQ++PISQA+ANQRAGRAGRTGPG+ YRLYTI Q+E +ML  +VPEIQRTNL NVVLLLKSL V+DLM+F+FMD PPQDNILNSM  LW+ GALD  G LT+LGR M EFPLDP+LSK+L+ +   GCSEE LTIVSMLSVP+IF RP G +E++D A E                       YS +FC + +IH K+M++VREVRQQLKEIM + K++L SSG D+D++R C+CSA+F+QAAR KGL EYVNLR+G+ C LHPTSSLY MA+  DY++YHEL+MTSKE+M  VT VD  WLAE G +FYSVK++  S
Sbjct:  796 GMDALQLFPISQANANQRAGRAGRTGPGVAYRLYTIGQFEEEMLLSSVPEIQRTNLANVVLLLKSLGVRDLMRFHFMDPPPQDNILNSMCQLWVFGALDNTGGLTQLGRQMVEFPLDPALSKLLLVACDMGCSEEALTIVSMLSVPSIFFRPKGREEDADLAREN----------------------YSPSFCTRNFIHVKAMRKVREVRQQLKEIMEQHKVALCSSGTDWDVVRRCLCSAFFHQAARIKGLAEYVNLRTGMPCRLHPTSSLYGMAYTPDYIIYHELVMTSKEYMQCVTAVDGNWLAEYGSIFYSVKDQSLS 1095          
BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. TrEMBL
Match: A0A5K3F262 (Uncharacterized protein OS=Mesocestoides corti OX=53468 PE=4 SV=1)

HSP 1 Score: 517.694 bits (1332), Expect = 0.000e+0
Identity = 321/663 (48.42%), Postives = 435/663 (65.61%), Query Frame = 3
Query:  129 DNEEYIKRQNERKQRDEKRKIGESFGSKSKIKKIENIDRHKQWEEMTP-----STSLRDETGRYSE-IRTPMIDRSIKTPSVT---SWDEDDVTFKR-------PDTVTSFRRND-----------------KSEIKWSP------DKNEEELDREWYL-NDGME--YNPFPNFSDEVLEKKKQQLQRQTSIVAKSRKSARAKQFEETNRAWEESKLINSGAVVRKG----FNVEIDNEDTGEDRIHLHVHNILPPFLDGRIVFTKQFEPVIPVKDENSDMAKIAKNGSQMVKNYRDIKEKMKLQKRTLDMTGTNFGKLMGLEKK--ETLDKWDETNYKDSMKYCDKITDK----IALDSEQKSKIDDQRKRLPIFKVRRDLIRLIRENQVIVIVGETGSGKTTQLTQYLLEENYHHKGIIGCTQPXXXXXXXXXXXXXDEVGTKLGDKVGYSIRFEDCKSENTMIKYMTDGIXXXXXXXXXXXXAYSVIIMDEAHERTLNTDILFGLLREIIQRRSDLHLVVTSATMDAEKFSNFFGKCEIFKIPGRTFPVDVIYEKSSILDPVAAAVNKAVDTHLGADSKDGDILVFMSGQEDVEVTCDKIDETLEKVKDTNPPDLKVEPLYSQLSSERQSEIFKINTSGIRRCIVATNIAETSVTLDGIR 1961
            D+ ++ + Q+ R +R   R      GS+   ++I    R + WE  TP     S      +  YS  I TP   R   TP+ +   +W+ DD++ K+       P    SF  N                   ++  ++P      +   E LDR WYL +DG +   NPF +  DE + K+++QL        K R SARA Q ++ N+AWE ++++ SG V R         E D ++ GE RIHL V NI+PPFLDGRI+FTK  EPV+PVKD +  M K+A  GS MV+ +R+ KE+ + QK+  ++ GT  G++MG+++   E  DKW E +Y++S  + DK+ D+    ++  ++QK+ I +QR+ LPIF VR  L+RLI+E+Q++VIVGETGSGKTTQLTQYL EE + + G++GCTQPRRVAA+SVAKRVADE+G  LGD+VGY IRFEDC +E T+IKYMTDGILLRESLR+ DLD YS IIMDEAHER+LNTD+LFGLLREI+ RR+DL L++TSATMD+EKF+ FFG C IF IPGRTFPVD+ + K+ ++D V AAV +A+  HLGA   +GDILVFM+GQED+EVTC+ I E L  + D  PP L V P+YSQL S+ Q++IF+     IR+C+VATNIAETS+T+DGIR
Sbjct:  132 DDRDHHRHQSGRNERASSR-----IGSEESARRI----RERDWEAETPRSGDSSHRSHGTSSSYSRYIETPRHLRPSDTPTPSRSAAWENDDLSEKKRTRYDYTPMQTPSFMHNSWMKGGGRGKQVAPTPATGNQTPFTPVDPQEMEAEAERLDRNWYLMDDGYDEGNNPFIDVPDEYVAKRERQLAEARK--RKIRMSARAVQIQKENQAWEINRMLRSGVVERIAGAEGGLDEEDLDEEGEARIHLLVKNIMPPFLDGRIIFTKHPEPVLPVKDPDGVMTKVAAKGSAMVRYFREQKERRRAQKKEWELAGTRIGEIMGIKQADNEEGDKWHEGSYRESQTFADKMVDQKDQAVSAFAKQKT-IKEQRQYLPIFGVRSALLRLIKEHQIVVIVGETGSGKTTQLTQYLHEEGFTNYGMVGCTQPRRVAAMSVAKRVADEMGVCLGDEVGYVIRFEDCTNEKTVIKYMTDGILLRESLRESDLDPYSAIIMDEAHERSLNTDVLFGLLREIVSRRNDLKLIITSATMDSEKFAQFFGDCPIFHIPGRTFPVDIQFSKTPVMDYVDAAVKQAIQVHLGA--PEGDILVFMTGQEDIEVTCELIAERLGSL-DEAPP-LSVLPIYSQLPSDLQAKIFQKTEDNIRKCVVATNIAETSLTVDGIR 778          

HSP 2 Score: 474.552 bits (1220), Expect = 0.000e+0
Identity = 219/322 (68.01%), Postives = 267/322 (82.92%), Query Frame = 1
Query: 1960 GMDSLQIYPISQASANQRAGRAGRTGPGICYRLYTINQYENDMLPMTVPEIQRTNLTNVVLLLKSLNVQDLMKFYFMDSPPQDNILNSMYHLWMLGALDAVGNLTELGRTMSEFPLDPSLSKMLITSVKHGCSEEMLTIVSMLSVPAIFSRPTGHQEESDCAHEKFQVAESDHLTLLNVYRLWRESGYSANFCKKYYIHNKSMKRVREVRQQLKEIMIKLKMSLASSG-DFDMIRYCICSAYFNQAARWKGLGEYVNLRSGVVCHLHPTSSLYNMAFNADYVVYHELLMTSKEFMNYVTFVDARWLAELGHMFYSVKEKGQS 2922
            GMD+LQ++PISQA+ANQRAGRAGRTGPG+ YRLYTI Q+E +ML  +VPEIQRTNL NVVLLLKSL V+DLM+F+FMD PPQDNILNSM  LW+ GALD  G LT+LGR M EFPLDP+LSK+L+ +   GCSEE LTIVSMLSVP+IF RP G +E++D A EKFQV ESDHLT LNV+  WR+SGYS +FC + +IH K+M++VREVRQQLKEIM + K++L SSG D+D++R C+CSA+F+QAAR KGL EYVNLR+G+ C LHPTSSLY MA+  DY++YHEL+MTSKE+M  VT VD  WLAE G +FYSVK++  S
Sbjct:  796 GMDALQLFPISQANANQRAGRAGRTGPGVAYRLYTIGQFEEEMLLSSVPEIQRTNLANVVLLLKSLGVRDLMRFHFMDPPPQDNILNSMCQLWVFGALDNTGGLTQLGRQMVEFPLDPALSKLLLVACDMGCSEEALTIVSMLSVPSIFFRPKGREEDADLAREKFQVPESDHLTYLNVFIRWRKSGYSPSFCTRNFIHVKAMRKVREVRQQLKEIMEQHKVALCSSGTDWDVVRRCLCSAFFHQAARIKGLAEYVNLRTGMPCRLHPTSSLYGMAYTPDYIIYHELVMTSKEYMQCVTAVDGNWLAEYGSIFYSVKDQSLS 1117          
BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. TrEMBL
Match: A0A1W4X3L2 (pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 isoform X1 OS=Agrilus planipennis OX=224129 GN=LOC108740730 PE=4 SV=1)

HSP 1 Score: 515.768 bits (1327), Expect = 0.000e+0
Identity = 296/559 (52.95%), Postives = 397/559 (71.02%), Query Frame = 3
Query:  336 SIKTPSVTSWDEDDVTFKRPDTVTSFRRNDKSEIKWS-------PDKNEEELDREWY-LNDGM--EYNPFPNFSDEVLEKKKQQLQRQTSIVAKSRKSARAKQFEETNRAWEESKLINSGAVVRKGFNVEIDNEDTGEDRIHLHVHNILPPFLDGRIVFTKQFEPVIPVKDENSDMAKIAKNGSQMVKNYRDIKEKMKLQKRTLDMTGTNFGKLMGLEKKETLDKW------DETNYKDSMKYCDKI--TDKIALDSEQKSKIDDQRKRLPIFKVRRDLIRLIRENQVIVIVGETGSGKTTQLTQYLLEENYHHKGIIGCTQPXXXXXXXXXXXXXDEVGTKLGDKVGYSIRFEDCKSENTMIKYMTDGIXXXXXXXXXXXXAYSVIIMDEAHERTLNTDILFGLLREIIQRRSDLHLVVTSATMDAEKFSNFFGKCEIFKIPGRTFPVDVIYEKSSILDPVAAAVNKAVDTHLGADSKDGDILVFMSGQEDVEVTCDKIDETLEKVKDTNPPDLKVEPLYSQLSSERQSEIFKINTSGIRRCIVATNIAETSVTLDGI 1958
            S +TP VT   + +V  K      +       E+KW         ++ ++ +DREWY L++G   E NPF N SD+  +KK++QL+++     K R SA+ +Q  + N  WE ++++ SG V    FN + D E    DR+HL VHNI+PPFLDGRIVFTKQ EPVIPV+D  SDMA  A+ GS++VK YR+ KE+ K QK+  ++ GT  G +MG++KKE  +        D+T+Y+   K+ D +  T+  + D  +K  I +QR+ LP+F VR++L+ +IREN V++IVGETGSGKTTQLTQYL E+ Y   G+IGCTQPRRVAA+SVAKRV+DE+GT+LGD+VGY+IRFEDC SENT+IKYMTDGILLRESLR+ +LD YS IIMDEAHER+L+TD+LFGLLREI+ +R DL L+VTSATMD+ KFS FFG    F IPGRTFPV+V++ K+ + D V AAV +A+  HL   S  GDIL+FM GQED+EVTC+ I E L ++   N P+L + P+YSQL S+ Q++IF+ +  GIR+C+VATNIAETS+T+DGI
Sbjct:  253 SWETPRVTPAYKFNVWVKDRKKTGATPGVSSEELKWQDEEDKELWEEEQKRIDREWYNLDEGYDDENNPFANVSDDYTKKKEEQLEQR----KKKRMSAQQRQINKDNELWERNRMLTSGVVHSVDFNEDFDEESL--DRVHLLVHNIVPPFLDGRIVFTKQPEPVIPVRDPTSDMALTARKGSRLVKIYREQKERKKAQKKHWELGGTKIGNIMGIKKKEDEEDARYNKDEDKTDYRTDQKFADHMNKTEGGSSDFAKKKTILEQRRYLPVFAVRQELLNVIRENSVVIIVGETGSGKTTQLTQYLHEDGYSKYGMIGCTQPRRVAAMSVAKRVSDEMGTQLGDEVGYAIRFEDCTSENTVIKYMTDGILLRESLREAELDHYSAIIMDEAHERSLSTDVLFGLLREIVAKRHDLKLIVTSATMDSSKFSLFFGNVPTFTIPGRTFPVEVLFSKNPVEDYVDAAVKQALQIHLQPPS--GDILIFMPGQEDIEVTCEVIAERLAEID--NSPELSILPIYSQLPSDLQAKIFQRSPEGIRKCVVATNIAETSLTVDGI 801          

HSP 2 Score: 469.929 bits (1208), Expect = 0.000e+0
Identity = 215/351 (61.25%), Postives = 279/351 (79.49%), Query Frame = 1
Query: 1960 GMDSLQIYPISQASANQRAGRAGRTGPGICYRLYTINQYENDMLPMTVPEIQRTNLTNVVLLLKSLNVQDLMKFYFMDSPPQDNILNSMYHLWMLGALDAVGNLTELGRTMSEFPLDPSLSKMLITSVKHGCSEEMLTIVSMLSVPAIFSRPTGHQEESDCAHEKFQVAESDHLTLLNVYRLWRESGYSANFCKKYYIHNKSMKRVREVRQQLKEIMIKLKMSLASSG-DFDMIRYCICSAYFNQAARWKGLGEYVNLRSGVVCHLHPTSSLYNMAFNADYVVYHELLMTSKEFMNYVTFVDARWLAELGHMFYSVKEKGQSVTDRMLKEQQTVERMETEMRINEKANLKK 3009
            GMD+LQIYPISQA+ANQR+GRAGRTGPG  +RLYT  QY+ ++L   VPEIQRTNL N VLLLKSL V+DL++F+FMD PPQDNILNS+Y LW+LGALD  G LT+LGR M+EFPLDP   +MLI S + GC+EE+LTIVSMLSVP+IF RP G +EE+D   EKFQV ESDHLT LNVY  W+++ YS+ +C +++IH K+M++VREVRQQLK+I+++ K+ + S G D+D++R CICSAYF+QAAR KG+GEYVNLR+G+ CHLHPTS+L+ +    DYVVYHEL+MT++E+M  VT VD  WLAELG MF+SVKE G+S   +  +  + +  ME +M++ +K  L +
Sbjct:  820 GMDALQIYPISQANANQRSGRAGRTGPGQAFRLYTERQYKEELLVTNVPEIQRTNLANTVLLLKSLGVEDLLQFHFMDPPPQDNILNSLYQLWILGALDNTGILTKLGRQMAEFPLDPPQCQMLIVSSQMGCTEEILTIVSMLSVPSIFYRPKGREEEADSVREKFQVPESDHLTYLNVYNQWKQNNYSSTWCNEHFIHIKAMRKVREVRQQLKDIVVQQKLEIISCGTDWDIVRKCICSAYFHQAARLKGIGEYVNLRTGMPCHLHPTSALFGLGNTPDYVVYHELVMTAREYMQCVTSVDGHWLAELGPMFFSVKETGKSGRAKKKQAAEHLIEMENQMQLAQKEMLAR 1170          
BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. TrEMBL
Match: A0A3Q3ACP3 (DEAH (Asp-Glu-Ala-His) box polypeptide 38 OS=Kryptolebias marmoratus OX=37003 PE=4 SV=1)

HSP 1 Score: 512.301 bits (1318), Expect = 0.000e+0
Identity = 302/612 (49.35%), Postives = 413/612 (67.48%), Query Frame = 3
Query:  243 RHKQWEEMTPSTSLRDE--------TGRYSEIRTPMI------DRSIKTPSV--TSWDEDDVTF-----------KRPDTVTSFRRNDKSEI-KWSPDKNEEELDREWYLND-GME--YNPFPNFSDEVLEKKKQQLQRQTSIVAKSRKSARAKQFEETNRAWEESKLINSGAVVRKGFNVEIDNEDTGEDRIHLHVHNILPPFLDGRIVFTKQFEPVIPVKDENSDMAKIAKNGSQMVKNYRDIKEKMKLQKRTLDMTGTNFGKLMGLEKKE-------TLDKWDETNYKDSMKYCDKITDKIALDSE--QKSKIDDQRKRLPIFKVRRDLIRLIRENQVIVIVGETGSGKTTQLTQYLLEENYHHKGIIGCTQPXXXXXXXXXXXXXDEVGTKLGDKVGYSIRFEDCKSENTMIKYMTDGIXXXXXXXXXXXXAYSVIIMDEAHERTLNTDILFGLLREIIQRRSDLHLVVTSATMDAEKFSNFFGKCEIFKIPGRTFPVDVIYEKSSILDPVAAAVNKAVDTHLGADSKDGDILVFMSGQEDVEVTCDKIDETLEKVKDTNPPDLKVEPLYSQLSSERQSEIFKINTSGIRRCIVATNIAETSVTLDGI 1958
            RH QWE  +P+ S R+         +GR SE R   +      D  + TPS     W  D               KR D    F  +D+ E  +W  D  +++ DR+WY+ D G +  +NPF + SD+ ++K++Q LQ+QT    + R SA+ +Q  E N  WE ++++ SG V R    V+ D E+    ++HL VHN++PPFLDGRIVFTKQ EPVIPVKD  SDMA I++ GSQ+V+ +R+ KE+ K Q +  ++ GT  G +MG++K E        + +  + +Y+   K+ + + +K    SE  +K  I +QR+ LPIF VR+ L+ +IR+N ++++VGETGSGKTTQLTQYL E+ Y   G++GCTQPRRVAA+SVAKRV++E+GT LG++VGY+IRFEDC SE T+IKYMTDGILLRESLR+ DLD YS +IMDEAHER+LNTD+LFGLLRE++ RR+DL L+VTSATMD++KF+ FFG   IF IPGRTFPVDV++ K+   D V AAV +A+  HL   +  GDIL+FM GQED+EVT D+I E LE ++  N P L V P+YSQL S+ Q++IF+    G+R+CIVATNIAETS+T+DGI
Sbjct:  245 RHSQWESPSPAPSHRESDRSERSHRSGRESERRDRSVRGRYSDDTPLPTPSYKYNEWANDRKHLGSTPRLSQGKAKREDGEGGFMFDDEDEKEQWEED--QKQADRDWYMMDEGYDEFHNPFTSTSDDYVKKREQILQKQT----QKRISAQKRQINEDNERWETNRMLTSGVVQR--LEVDEDFEEDNAAKVHLLVHNLVPPFLDGRIVFTKQPEPVIPVKDATSDMAIISRKGSQLVRKHREQKERKKAQHKHWELAGTKLGDIMGIKKTEDEEPSGKVVGEDGKVDYRADQKFAEHMKEKSEASSEFAKKKSILEQRQYLPIFAVRQQLLNIIRDNSIVIVVGETGSGKTTQLTQYLHEDGYTRYGMVGCTQPRRVAAMSVAKRVSEEIGTNLGEEVGYAIRFEDCTSEKTLIKYMTDGILLRESLRESDLDHYSAVIMDEAHERSLNTDVLFGLLREVVSRRTDLKLIVTSATMDSDKFAGFFGNVPIFHIPGRTFPVDVLFSKTPQEDYVEAAVKQALQIHLSGMA--GDILIFMPGQEDIEVTSDQIVERLEDLE--NAPALAVLPIYSQLPSDLQAKIFQKAPDGVRKCIVATNIAETSLTVDGI 844          

HSP 2 Score: 478.789 bits (1231), Expect = 0.000e+0
Identity = 219/346 (63.29%), Postives = 274/346 (79.19%), Query Frame = 1
Query: 1960 GMDSLQIYPISQASANQRAGRAGRTGPGICYRLYTINQYENDMLPMTVPEIQRTNLTNVVLLLKSLNVQDLMKFYFMDSPPQDNILNSMYHLWMLGALDAVGNLTELGRTMSEFPLDPSLSKMLITSVKHGCSEEMLTIVSMLSVPAIFSRPTGHQEESDCAHEKFQVAESDHLTLLNVYRLWRESGYSANFCKKYYIHNKSMKRVREVRQQLKEIMIKLKMSLASSG-DFDMIRYCICSAYFNQAARWKGLGEYVNLRSGVVCHLHPTSSLYNMAFNADYVVYHELLMTSKEFMNYVTFVDARWLAELGHMFYSVKEKGQSVTDRMLKEQQTVERMETEMRINEK 2994
            GMD+LQ+YPISQA+ANQR+GRAGRTGPG CYRLYT + Y+N+ML  T+PEIQRTNL NVVLLLKSL VQDL+ F+FMD PP+DN+LNSMY LW+LGALD  G LT  GR M EFPLDP+LSKMLI S   GCS ++L IVSMLSVPAIF RP G +EESD   EKF V ESDHLT LNVY  W+ + YS+ +C  ++IH K+M++VREVR QLK+IM++ KM+L S G D+D+IR CIC+AYF+QAA+ KG+GEYVN+R+G+ CHLHPTSSL+ M +  DY++YHEL+MT+KE+M  VT VD  WLAELG MFYS+K  G+S  +   + ++ +  ME EM + E+
Sbjct:  863 GMDALQVYPISQANANQRSGRAGRTGPGQCYRLYTQSAYKNEMLTTTIPEIQRTNLANVVLLLKSLGVQDLLLFHFMDPPPEDNMLNSMYQLWILGALDNTGALTPTGRLMVEFPLDPALSKMLIVSCDMGCSADILIIVSMLSVPAIFYRPKGREEESDQVREKFSVPESDHLTYLNVYMQWKNNNYSSIWCNDHFIHTKAMRKVREVRSQLKDIMVQQKMNLVSCGSDWDIIRKCICAAYFHQAAKLKGIGEYVNVRTGMPCHLHPTSSLFGMGYTPDYIIYHELVMTTKEYMQCVTAVDGEWLAELGPMFYSIKHAGKSRQENRRRAKEEINNMEQEMVLAEE 1208          
BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. TrEMBL
Match: A0A3Q2DLV6 (DEAH (Asp-Glu-Ala-His) box polypeptide 38 OS=Cyprinodon variegatus OX=28743 PE=4 SV=1)

HSP 1 Score: 511.916 bits (1317), Expect = 0.000e+0
Identity = 300/612 (49.02%), Postives = 413/612 (67.48%), Query Frame = 3
Query:  243 RHKQWEEMTPSTSLRDE--------TGRYSEIRTPMIDRSIK----------TPSV--TSWDEDDVTFKRPDTVTSFRRN--------DKSEIKWSPDKNEEELDREWYLND-GME--YNPFPNFSDEVLEKKKQQLQRQTSIVAKSRKSARAKQFEETNRAWEESKLINSGAVVRKGFNVEIDNEDTGEDRIHLHVHNILPPFLDGRIVFTKQFEPVIPVKDENSDMAKIAKNGSQMVKNYRDIKEKMKLQKRTLDMTGTNFGKLMGLEKKE-------TLDKWDETNYKDSMKYCDKITDK--IALDSEQKSKIDDQRKRLPIFKVRRDLIRLIRENQVIVIVGETGSGKTTQLTQYLLEENYHHKGIIGCTQPXXXXXXXXXXXXXDEVGTKLGDKVGYSIRFEDCKSENTMIKYMTDGIXXXXXXXXXXXXAYSVIIMDEAHERTLNTDILFGLLREIIQRRSDLHLVVTSATMDAEKFSNFFGKCEIFKIPGRTFPVDVIYEKSSILDPVAAAVNKAVDTHLGADSKDGDILVFMSGQEDVEVTCDKIDETLEKVKDTNPPDLKVEPLYSQLSSERQSEIFKINTSGIRRCIVATNIAETSVTLDGI 1958
            RH QWE  +P+ S RD         TGR SE R    DRS++          TPS     W  D         ++  +          D  E K   ++++++ DR+WY+ D G +  +NPF + SDE ++K++Q LQ+QT    + R SA+ +Q  E N  WE ++++ SG V R    V+ D E+    ++HL VHN++PPFLDGRIVFTKQ EP++PVKD  SDMA I++ GSQ+V+ +R+ KE+ K Q +  ++ GT  G +MG++KKE        + +  + +Y+   K+ + + +K   + D  +K  I +QR+ LPIF VR+ L+ +IR+N ++++VGETGSGKTTQLTQYL E+ Y   G++GCTQPRRVAA+SVAKRV++E+GT LG++VGY+IRFEDC SE TMIKYMTDGILLRESLR+ DLD YS +IMDEAHER+LNTD+LFGLLRE++ RR+DL L+VTSATMD++KF+ FFG   IF IPGRTFPVD+++ K+   D V AAV +A+  HL      GDIL+FM GQED+EVT D+I E LE ++  N P L V P+YSQL S+ Q++IF+    G+R+CIVATNIAETS+T+DGI
Sbjct:  248 RHSQWESPSPAPSQRDSDRSERSYRTGRESERR----DRSVRGRYTDNTPLPTPSYKYNEWANDRKHLGSTPRLSQGKGKKEEGGMMFDDQEEKEQWEEDQKQADRDWYMMDEGYDEFHNPFTSTSDEYVKKREQILQKQT----QKRISAQKRQINEDNERWETNRMLTSGVVQR--LEVDEDFEEDNAAKVHLLVHNLVPPFLDGRIVFTKQPEPIVPVKDPTSDMAIISRKGSQLVRKHREQKERKKAQHKHWELAGTKLGDIMGIKKKEEEEPGGKVVGEDGKVDYRADQKFAEHMKEKSEASSDFAKKKSILEQRQYLPIFAVRQQLLNIIRDNSIVIVVGETGSGKTTQLTQYLHEDGYTRYGMVGCTQPRRVAAMSVAKRVSEEIGTNLGEEVGYAIRFEDCTSEKTMIKYMTDGILLRESLRESDLDHYSAVIMDEAHERSLNTDVLFGLLREVVARRTDLKLIVTSATMDSDKFAAFFGNVPIFHIPGRTFPVDILFSKTPQEDYVEAAVKQALQIHLSG--MVGDILIFMPGQEDIEVTSDQIVERLEDLE--NAPPLAVLPIYSQLPSDLQAKIFQKAPDGVRKCIVATNIAETSLTVDGI 845          

HSP 2 Score: 476.093 bits (1224), Expect = 0.000e+0
Identity = 218/341 (63.93%), Postives = 271/341 (79.47%), Query Frame = 1
Query: 1960 GMDSLQIYPISQASANQRAGRAGRTGPGICYRLYTINQYENDMLPMTVPEIQRTNLTNVVLLLKSLNVQDLMKFYFMDSPPQDNILNSMYHLWMLGALDAVGNLTELGRTMSEFPLDPSLSKMLITSVKHGCSEEMLTIVSMLSVPAIFSRPTGHQEESDCAHEKFQVAESDHLTLLNVYRLWRESGYSANFCKKYYIHNKSMKRVREVRQQLKEIMIKLKMSLASSG-DFDMIRYCICSAYFNQAARWKGLGEYVNLRSGVVCHLHPTSSLYNMAFNADYVVYHELLMTSKEFMNYVTFVDARWLAELGHMFYSVKEKGQSVTDRMLKEQQTVERMETEM 2979
            GMD+LQ+YPISQA+ANQR+GRAGRTGPG CYRLYT + Y+N+ML  T+PEIQRTNL NVVLLLKSL VQDL+ F+FMD PP+DN+LNSMY LW+LGALD  G LT  GR M EFPLDP+LSKMLI S   GCS ++L IVSMLSVPAIF RP G +EESD   EKF V ESDHLT LNVY  W+ + YS+ +C  ++IH K+M++VREVR QLK+IM++ KM+L S G D+D+IR CIC+AYF+QAA+ KG+GEYVN+R+G+ CHLHPTSSL+ M +  DY++YHEL+MT+KE+M  VT VD  WLAELG MFYS+K  G+S  +   + ++ +  ME EM
Sbjct:  864 GMDALQVYPISQANANQRSGRAGRTGPGQCYRLYTQSAYKNEMLTTTIPEIQRTNLANVVLLLKSLGVQDLLLFHFMDPPPEDNMLNSMYQLWILGALDNTGALTPTGRLMVEFPLDPALSKMLIVSCDMGCSADILIIVSMLSVPAIFYRPKGREEESDQVREKFSVPESDHLTYLNVYMQWKNNNYSSIWCNDHFIHTKAMRKVREVRSQLKDIMVQQKMNLISCGSDWDIIRKCICAAYFHQAAKLKGIGEYVNVRTGMPCHLHPTSSLFGMGYTPDYIIYHELVMTTKEYMQCVTAVDGEWLAELGPMFYSIKHAGKSRQENRRRAKEELTNMEQEM 1204          
BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. Ensembl Cavefish
Match: dhx38 (DEAH (Asp-Glu-Ala-His) box polypeptide 38 [Source:ZFIN;Acc:ZDB-GENE-040426-1144])

HSP 1 Score: 477.248 bits (1227), Expect = 2.885e-149
Identity = 226/372 (60.75%), Postives = 284/372 (76.34%), Query Frame = 1
Query: 1960 GMDSLQIYPISQASANQRAGRAGRTGPGICYRLYTINQYENDMLPMTVPEIQRTNLTNVVLLLKSLNVQDLMKFYFMDSPPQDNILNSMYHLWMLGALDAVGNLTELGRTMSEFPLDPSLSKMLITSVKHGCSEEMLTIVSMLSVPAIFSRPTGHQEESDCAHEKFQVAESDHLTLLNVYRLWRESGYSANFCKKYYIHNKSMKRVREVRQQLKEIMIKLKMSLASSG-DFDMIRYCICSAYFNQAARWKGLGEYVNLRSGVVCHLHPTSSLYNMAFNADYVVYHELLMTSKEFMNYVTFVDARWLAELGHMFYSVKEKGQSVTDRMLKEQQTVERMETEMRINEKANLKKLLDNRVIESERRAQSVKETTY 3072
            GMD+LQ+YPISQA+ANQRAGRAGRTGPG CYRLYT + ++N+ML  T+PEIQRTNL NVVLLLKSL VQDL+ F+FMD PP+DN+LNSMY LW+LGALD  G LT  GR M EFPLDP+LSKMLI S   GCS ++L IVSMLSVP IF RP G +EESD   EKF V ESDHLT LNVY  W+ + YS+ +C +++IH K+M++VREVR QLK+IM++ KM+L S G D+D+IR CIC+AYF+QAA+ KG+GEYVN+R+G+ CHLHPTSSL+ M +  DY+ YHEL+MT+KE+M  VT VD  WLAELG MFYS+K  G+S  +   + ++ +  ME EM + E+      L  R  E ER++ S+  T Y
Sbjct:  825 GMDALQVYPISQANANQRAGRAGRTGPGQCYRLYTQSAFKNEMLTTTIPEIQRTNLANVVLLLKSLGVQDLLLFHFMDPPPEDNMLNSMYQLWILGALDNTGALTPTGRLMVEFPLDPALSKMLIVSCDMGCSADILIIVSMLSVPTIFYRPKGREEESDQVREKFAVPESDHLTYLNVYLQWKNNNYSSIWCNEHFIHTKAMRKVREVRAQLKDIMVQQKMNLTSCGSDWDVIRKCICAAYFHQAAKLKGIGEYVNVRTGMPCHLHPTSSLFGMGYTPDYITYHELVMTTKEYMQCVTAVDGEWLAELGPMFYSIKHAGKSRQENRRRAKEEITNMEEEMSLAEQQ-----LRARREEQERKS-SLGSTRY 1190          

HSP 2 Score: 473.396 bits (1217), Expect = 5.803e-148
Identity = 281/567 (49.56%), Postives = 386/567 (68.08%), Query Frame = 3
Query:  408 SFRRNDKSEIKWSPDKNEEELDREWYLND-GME--YNPFPNFSDEVLEKKKQQLQRQTSIVAKSRKSARAKQFEETNRAWEESKLINSGAVVRKGFNVEIDNEDTGEDRIHLHVHNILPPFLDGRIVFTKQFEPVIPVKDENSDMAKIAKNGSQMVKNYRDIKEKMKLQKRTLDMTGTNFGKLMGLEKKE------TLDKWDETNYKDSMKYCDKITDKIALDSE--QKSKIDDQRKRLPIFKVRRDLIRLIRENQVIVIVGETGSGKTTQLTQYLLEENYHHKGIIGCTQPXXXXXXXXXXXXXDEVGTKLGDKVGYSIRFEDCKSENTMIKYMTDGIXXXXXXXXXXXXAYSVIIMDEAHERTLNTDILFGLLREIIQRRSDLHLVVTSATMDAEKFSNFFGKCEIFKIPGRTFPVDVIYEKSSILDPVAAAVNKAVDTHLGADSKDGDILVFMSGQEDVEVTCDKIDETLEKVKDTNPPDLKVEPLYSQLSSERQSEIFKINTSGIRRCIVATNIAETSVTLDGI----------------RNGLVADISHQSSQC*SKSRAGR 2027
            SF   D+ E +W  D  + + DR+WY+ D G +  +NP  + SDE ++K++Q LQ+QT    + R SA+ +Q  E N  WE ++++ SG V R    V+ D E+    ++HL VHN++PPFLDGRIVFTKQ EPVIPVKD  SDMA I++ GSQ+V+ +R+ KE+ K Q +  ++ GT  G +MG++KKE       + +    +Y+   K+ D + +K    SE  +K  + +QR+ LPIF VR++L+ +IR+N ++++VGETGSGKTTQLTQYL E+ Y   G++GCTQPRRVAA+SVAKRV++E+G+ LG++VGY+IRFEDC SE T+IKYMTDGILLRESLR+ DLD YS +IMDEAHER+LNTD+LFGLLRE++ RRSDL L+VTSATMD++KF+              TFPVD+++ K+   D V AAV +A+  HL      GDIL+FM GQED+EVT D+I E LE ++  N P L V P+YSQL S+ Q++IF+    G+R+CIVATNIAETS+T+DGI                R G+ A   +  SQ  +  RAGR
Sbjct:  305 SFDNEDEKE-QWEED--QRQADRDWYMMDEGYDEFHNPLTSSSDEYVQKREQILQKQT----QKRISAQKRQINEDNERWETNRMLTSGVVQR--LEVDEDFEEDNATKVHLLVHNLVPPFLDGRIVFTKQPEPVIPVKDATSDMAIISRKGSQLVRKHREQKERKKAQHKHWELAGTKLGDIMGIKKKEDGSEEKPVGEDGAVDYRAEQKFADHMKEKTEASSEFAKKKTLLEQRQYLPIFAVRQELLNIIRDNSIVIVVGETGSGKTTQLTQYLHEDGYTSYGMVGCTQPRRVAAMSVAKRVSEEIGSNLGEEVGYAIRFEDCTSEKTVIKYMTDGILLRESLRESDLDHYSAVIMDEAHERSLNTDVLFGLLREVVSRRSDLKLIVTSATMDSDKFAA-------------TFPVDILFSKTPQEDYVEAAVKQALQIHLSG--MVGDILIFMPGQEDIEVTSDQIVERLEDLE--NAPPLAVLPIYSQLPSDLQAKIFQKAPDGVRKCIVATNIAETSLTVDGIMFVVDSGYCKLKVFNPRIGMDALQVYPISQANANQRAGR 845          
BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. Ensembl Cavefish
Match: dhx15 (DEAH-box helicase 15 [Source:NCBI gene;Acc:103038591])

HSP 1 Score: 242.662 bits (618), Expect = 6.728e-128
Identity = 130/343 (37.90%), Postives = 206/343 (60.06%), Query Frame = 1
Query: 1963 MDSLQIYPISQASANQRAGRAGRTGPGICYRLYTINQYENDMLPMTVPEIQRTNLTNVVLLLKSLNVQDLMKFYFMDSPPQDNILNSMYHLWMLGALDAVGNLTELGRTMSEFPLDPSLSKMLITSVKHGCSEEMLTIVSMLSVPAIFSRPTGHQEESDCAHEKFQVAESDHLTLLNVYRLWRESGYSANFCKKYYIHNKSMKRVREVRQQLKEIMIKLKMSLAS----SGDFDM-IRYCICSAYFNQAARWKGLGEYVNLRSGVVCHLHPTSSLYNMAFNADYVVYHELLMTSKEFMNYVTFVDARWLAELGHMFYSVKEKGQSVTDRMLKEQQTVERMETE 2976
            ++SL +  IS+ASA QRAGRAGRT PG C+RLYT   Y+ +M   T PEI R+NL +VVL LK L + DL+ F FMD P  + ++ ++  L  L AL+  G+LTELG  M+EFPLDP L+KM+I S +  CS E+L+I +MLSVP  F RPT  ++ +D +  +F   + DHLTLLNVY  ++++  S  +C   +++ +S+     VRQQL  IM +  +   S    S D+ + IR  + + +F Q A  +  G Y+ ++   V  LHP++ L +     ++V+Y+E ++T+K ++   T +   WL ++   +Y +    Q    R L  ++ + +++T+
Sbjct:  421 VESLLVTAISKASAQQRAGRAGRTRPGKCFRLYTEKAYKTEMQDNTYPEILRSNLGSVVLQLKKLGIDDLVHFDFMDPPAPETLMRALELLNYLAALNDDGDLTELGSMMAEFPLDPQLAKMVIASCEFNCSNEILSITAMLSVPQCFVRPTEAKKAADESKMRFAHIDGDHLTLLNVYHAFKQNHESVQWCYDNFVNYRSLMSADNVRQQLSRIMDRFNLPRRSTEFTSRDYYINIRRALVTGFFMQVAHLERTGHYLTVKDNQVVQLHPSTVLDH---KPEWVLYNEFVLTTKNYIRTCTDIKPEWLVKIAPQYYEMSNFPQCEAKRQL--ERIIAKLQTK 758          

HSP 2 Score: 236.884 bits (603), Expect = 6.728e-128
Identity = 142/309 (45.95%), Postives = 209/309 (67.64%), Query Frame = 3
Query: 1065 KIDDQRKRLPIFKVRRDLIRLIRENQVIVIVGETGSGKTTQLTQYLLEENYHHKGI---IGCTQPXXXXXXXXXXXXXDEVGTKLGDKVGYSIRFEDCKSENTMIKYMTDGIXXXXXXXXXXXXAYSVIIMDEAHERTLNTDILFGLLREIIQRRSDLHLVVTSATMDAEKFSNFFGKCEIFKIPGRTFPVDVIYEKSSILDPVAAAVNKAVDTHLGADSKDGDILVFMSGQEDVEVTCDKIDETLEKVKDTNP--PDLKVEPLYSQLSSERQSEIFKI----NTSGI--RRCIVATNIAETSVTLDGI 1958
            +I  +R +LP+++ +     ++  +Q  V+VGETGSGKTTQ+ Q+ ++      G    + CTQPRRVAA+SVA+RVADE+   LG +VGYSIRFEDC S  T++KYMTDG+LLRE++ D  L+ Y VII+DEAHERTL TDIL G+L+E++++RSDL ++V SAT+DA KF  +F  C +  IPGRT PV++ Y      D + AA+   +  H+  +  +GD+L+F++GQE+++  C +I     ++ D  P   D+K+ PLYS L  ++Q  IF+       SG   R+ +V+TNIAETS+T+DG+
Sbjct:   97 EILKKRLQLPVWEYKERFNDILTRHQSFVLVGETGSGKTTQIPQWCVDMVRSLPGPKRGVACTQPRRVAAMSVAQRVADEMDVMLGQEVGYSIRFEDCSSAKTILKYMTDGMLLREAMNDPLLERYGVIILDEAHERTLATDILMGVLKEVVRQRSDLKVIVMSATLDAGKFQVYFDSCPLLTIPGRTHPVEIFYTPEPERDYLEAAIRTVIQIHM-CEEDEGDVLLFLTGQEEIDEACKRIK---REIDDLGPDVGDIKIIPLYSTLPPQQQQRIFEPPPPRKPSGAIGRKVVVSTNIAETSLTIDGV 401          
BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. Ensembl Cavefish
Match: dhx15 (DEAH-box helicase 15 [Source:NCBI gene;Acc:103038591])

HSP 1 Score: 236.884 bits (603), Expect = 1.921e-125
Identity = 142/309 (45.95%), Postives = 209/309 (67.64%), Query Frame = 3
Query: 1065 KIDDQRKRLPIFKVRRDLIRLIRENQVIVIVGETGSGKTTQLTQYLLEENYHHKGI---IGCTQPXXXXXXXXXXXXXDEVGTKLGDKVGYSIRFEDCKSENTMIKYMTDGIXXXXXXXXXXXXAYSVIIMDEAHERTLNTDILFGLLREIIQRRSDLHLVVTSATMDAEKFSNFFGKCEIFKIPGRTFPVDVIYEKSSILDPVAAAVNKAVDTHLGADSKDGDILVFMSGQEDVEVTCDKIDETLEKVKDTNP--PDLKVEPLYSQLSSERQSEIFKI----NTSGI--RRCIVATNIAETSVTLDGI 1958
            +I  +R +LP+++ +     ++  +Q  V+VGETGSGKTTQ+ Q+ ++      G    + CTQPRRVAA+SVA+RVADE+   LG +VGYSIRFEDC S  T++KYMTDG+LLRE++ D  L+ Y VII+DEAHERTL TDIL G+L+E++++RSDL ++V SAT+DA KF  +F  C +  IPGRT PV++ Y      D + AA+   +  H+  +  +GD+L+F++GQE+++  C +I     ++ D  P   D+K+ PLYS L  ++Q  IF+       SG   R+ +V+TNIAETS+T+DG+
Sbjct:   97 EILKKRLQLPVWEYKERFNDILTRHQSFVLVGETGSGKTTQIPQWCVDMVRSLPGPKRGVACTQPRRVAAMSVAQRVADEMDVMLGQEVGYSIRFEDCSSAKTILKYMTDGMLLREAMNDPLLERYGVIILDEAHERTLATDILMGVLKEVVRQRSDLKVIVMSATLDAGKFQVYFDSCPLLTIPGRTHPVEIFYTPEPERDYLEAAIRTVIQIHM-CEEDEGDVLLFLTGQEEIDEACKRIK---REIDDLGPDVGDIKIIPLYSTLPPQQQQRIFEPPPPRKPSGAIGRKVVVSTNIAETSLTIDGV 401          

HSP 2 Score: 234.572 bits (597), Expect = 1.921e-125
Identity = 124/308 (40.26%), Postives = 189/308 (61.36%), Query Frame = 1
Query: 1963 MDSLQIYPISQASANQRAGRAGRTGPGICYRLYTINQYENDMLPMTVPEIQRTNLTNVVLLLKSLNVQDLMKFYFMDSPPQDNILNSMYHLWMLGALDAVGNLTELGRTMSEFPLDPSLSKMLITSVKHGCSEEMLTIVSMLSVPAIFSRPTGHQEESDCAHEKFQVAESDHLTLLNVYRLWRESGYSANFCKKYYIHNKSMKRVREVRQQLKEIMIKLKMSLAS----SGDFDM-IRYCICSAYFNQAARWKGLGEYVNLRSGVVCHLHPTSSLYNMAFNADYVVYHELLMTSKEFMNYVTFVDARW 2871
            ++SL +  IS+ASA QRAGRAGRT PG C+RLYT   Y+ +M   T PEI R+NL +VVL LK L + DL+ F FMD P  + ++ ++  L  L AL+  G+LTELG  M+EFPLDP L+KM+I S +  CS E+L+I +MLSVP  F RPT  ++ +D +  +F   + DHLTLLNVY  ++++  S  +C   +++ +S+     VRQQL  IM +  +   S    S D+ + IR  + + +F Q A  +  G Y+ ++   V  LHP++ L +     ++V+Y+E ++T+K ++   T +   W
Sbjct:  421 VESLLVTAISKASAQQRAGRAGRTRPGKCFRLYTEKAYKTEMQDNTYPEILRSNLGSVVLQLKKLGIDDLVHFDFMDPPAPETLMRALELLNYLAALNDDGDLTELGSMMAEFPLDPQLAKMVIASCEFNCSNEILSITAMLSVPQCFVRPTEAKKAADESKMRFAHIDGDHLTLLNVYHAFKQNHESVQWCYDNFVNYRSLMSADNVRQQLSRIMDRFNLPRRSTEFTSRDYYINIRRALVTGFFMQVAHLERTGHYLTVKDNQVVQLHPSTVLDH---KPEWVLYNEFVLTTKNYIRTCTDIKPEW 725          
BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. Ensembl Cavefish
Match: dhx15 (DEAH-box helicase 15 [Source:NCBI gene;Acc:103038591])

HSP 1 Score: 236.884 bits (603), Expect = 2.020e-125
Identity = 142/309 (45.95%), Postives = 209/309 (67.64%), Query Frame = 3
Query: 1065 KIDDQRKRLPIFKVRRDLIRLIRENQVIVIVGETGSGKTTQLTQYLLEENYHHKGI---IGCTQPXXXXXXXXXXXXXDEVGTKLGDKVGYSIRFEDCKSENTMIKYMTDGIXXXXXXXXXXXXAYSVIIMDEAHERTLNTDILFGLLREIIQRRSDLHLVVTSATMDAEKFSNFFGKCEIFKIPGRTFPVDVIYEKSSILDPVAAAVNKAVDTHLGADSKDGDILVFMSGQEDVEVTCDKIDETLEKVKDTNP--PDLKVEPLYSQLSSERQSEIFKI----NTSGI--RRCIVATNIAETSVTLDGI 1958
            +I  +R +LP+++ +     ++  +Q  V+VGETGSGKTTQ+ Q+ ++      G    + CTQPRRVAA+SVA+RVADE+   LG +VGYSIRFEDC S  T++KYMTDG+LLRE++ D  L+ Y VII+DEAHERTL TDIL G+L+E++++RSDL ++V SAT+DA KF  +F  C +  IPGRT PV++ Y      D + AA+   +  H+  +  +GD+L+F++GQE+++  C +I     ++ D  P   D+K+ PLYS L  ++Q  IF+       SG   R+ +V+TNIAETS+T+DG+
Sbjct:   77 EILKKRLQLPVWEYKERFNDILTRHQSFVLVGETGSGKTTQIPQWCVDMVRSLPGPKRGVACTQPRRVAAMSVAQRVADEMDVMLGQEVGYSIRFEDCSSAKTILKYMTDGMLLREAMNDPLLERYGVIILDEAHERTLATDILMGVLKEVVRQRSDLKVIVMSATLDAGKFQVYFDSCPLLTIPGRTHPVEIFYTPEPERDYLEAAIRTVIQIHM-CEEDEGDVLLFLTGQEEIDEACKRIK---REIDDLGPDVGDIKIIPLYSTLPPQQQQRIFEPPPPRKPSGAIGRKVVVSTNIAETSLTIDGV 381          

HSP 2 Score: 234.572 bits (597), Expect = 2.020e-125
Identity = 124/308 (40.26%), Postives = 189/308 (61.36%), Query Frame = 1
Query: 1963 MDSLQIYPISQASANQRAGRAGRTGPGICYRLYTINQYENDMLPMTVPEIQRTNLTNVVLLLKSLNVQDLMKFYFMDSPPQDNILNSMYHLWMLGALDAVGNLTELGRTMSEFPLDPSLSKMLITSVKHGCSEEMLTIVSMLSVPAIFSRPTGHQEESDCAHEKFQVAESDHLTLLNVYRLWRESGYSANFCKKYYIHNKSMKRVREVRQQLKEIMIKLKMSLAS----SGDFDM-IRYCICSAYFNQAARWKGLGEYVNLRSGVVCHLHPTSSLYNMAFNADYVVYHELLMTSKEFMNYVTFVDARW 2871
            ++SL +  IS+ASA QRAGRAGRT PG C+RLYT   Y+ +M   T PEI R+NL +VVL LK L + DL+ F FMD P  + ++ ++  L  L AL+  G+LTELG  M+EFPLDP L+KM+I S +  CS E+L+I +MLSVP  F RPT  ++ +D +  +F   + DHLTLLNVY  ++++  S  +C   +++ +S+     VRQQL  IM +  +   S    S D+ + IR  + + +F Q A  +  G Y+ ++   V  LHP++ L +     ++V+Y+E ++T+K ++   T +   W
Sbjct:  401 VESLLVTAISKASAQQRAGRAGRTRPGKCFRLYTEKAYKTEMQDNTYPEILRSNLGSVVLQLKKLGIDDLVHFDFMDPPAPETLMRALELLNYLAALNDDGDLTELGSMMAEFPLDPQLAKMVIASCEFNCSNEILSITAMLSVPQCFVRPTEAKKAADESKMRFAHIDGDHLTLLNVYHAFKQNHESVQWCYDNFVNYRSLMSADNVRQQLSRIMDRFNLPRRSTEFTSRDYYINIRRALVTGFFMQVAHLERTGHYLTVKDNQVVQLHPSTVLDH---KPEWVLYNEFVLTTKNYIRTCTDIKPEW 705          
BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. Ensembl Cavefish
Match: dhx33 (DEAH (Asp-Glu-Ala-His) box polypeptide 33 [Source:NCBI gene;Acc:796505])

HSP 1 Score: 228.794 bits (582), Expect = 1.329e-120
Identity = 123/320 (38.44%), Postives = 196/320 (61.25%), Query Frame = 1
Query: 1960 GMDSLQIYPISQASANQRAGRAGRTGPGICYRLYTINQYENDMLPMTVPEIQRTNLTNVVLLLKSLNVQDLMKFYFMDSPPQDNILNSMYHLWMLGALDAVGN---LTELGRTMSEFPLDPSLSKMLITSVKHGCSEEMLTIVSMLSVPAIFSRPTGHQEESDCAHEKFQVAESDHLTLLNVYRLWRESGYSANFCKKYYIHNKSMKRVREVRQQLKEIMIKLKMSLASS-GDFDMIRYCICSAYFNQAARWKGLGEYVNLRSGVVCHLHPTSSLYNMAFNADYVVYHELLMTSKEFMNYVTFVDARWLAELGHMFYSVK 2907
            G++ L +  +S+A A QRAGRAGR   G CYRLYT  +++N ++ MTVPEIQR NL  VVL L +L V D++ F FM  P  +++  ++  L +LGA++   +   LT LG+ M+ FPL+P  +K ++ S +  C+EE+LTI+S+LSV ++   P   ++E     +KF  +E DH+TLLN+YR +++   +  +C++ ++++++M  V EVR QL++I IKL + L SS  +   +R C+    F  AA  +  G YV L +     +HP+S L+       YVV++ELL TS+ +M  +  VD+ WL E    ++  K
Sbjct:  455 GLEVLAVQRVSKAQAWQRAGRAGREDSGSCYRLYTEEEFDN-LISMTVPEIQRCNLAGVVLQLLALGVPDVLNFDFMSKPSPESMRMAVEQLDLLGAVERKDDQVILTPLGKKMAYFPLEPRYAKTILISPEFSCTEEVLTILSLLSVDSVLYNPPARRDEVLAVRKKFISSEGDHMTLLNIYRAFKKVSGNKEWCRENFVNSRNMGLVAEVRSQLRDICIKLGLKLESSQSELVNVRRCLARGMFTNAAELQPDGSYVALDTHQPVSIHPSSVLFQA--KPAYVVFNELLHTSRCYMRDLCLVDSDWLQEAAPEYFRRK 771          

HSP 2 Score: 226.483 bits (576), Expect = 1.329e-120
Identity = 136/303 (44.88%), Postives = 195/303 (64.36%), Query Frame = 3
Query: 1068 IDDQRKRLPIFKVRRDLIRLIRENQVIVIVGETGSGKTTQLTQYLLEENYHHKGIIGCTQPXXXXXXXXXXXXXDEVGTKLGDKVGYSIRFEDCKSENTMIKYMTDGIXXXXXXXXXXXXAYSVIIMDEAHERTLNTDILFGLLREIIQRRSD-----LHLVVTSATMDAEKFSNFFGKCEIFKIPGRTFPVDVIYEKSSILDPVAAAVNKAVDTHLGADSKDGDILVFMSGQEDVEVTCDKIDETLEKVKDTNPPDLKVEPLYSQLSSERQSEIFKINTSGIRRCIVATNIAETSVTLDGIR 1961
            +D QRK+LPI++ R  L+  +R+    V++GETGSGKTTQ+ QYL E     +G+IG TQPRRVAA+S+A RVA E    LG  VGY++RFED  S  T IK+MTDG+LLRE++ D  L  Y+V+I+DEAHERT++TD+LFG+++   ++R +     L ++V SATMD + FS +F K  +  + GR  P+ + Y K    D + AA+      H  A S   DILVFM+GQE++E       +  + + DT  P + V PLY+ L   +Q  +F+    G R+ I++TNIAETS+T+ GI+
Sbjct:  137 LDSQRKQLPIYQARSQLLSQLRQLHNAVLIGETGSGKTTQIPQYLYEAGIGRQGLIGVTQPRRVAAISLAGRVAQEKNCSLGKLVGYTVRFEDVTSSETKIKFMTDGMLLREAIGDPLLLRYTVVILDEAHERTIHTDVLFGVVKSAQRKRKEHNKIPLKVLVMSATMDVDLFSQYFNKSPVLYLEGRQHPIQIYYTKQPQSDYLQAALVSIFQIHQEAPSSH-DILVFMTGQEEIEALARTCRDIAKHLPDTCGP-MTVIPLYASLPPTQQLRVFQPAPKGCRKVILSTNIAETSITISGIK 437          
BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. Ensembl Sea Lamprey
Match: dhx8 (DEAH (Asp-Glu-Ala-His) box polypeptide 8 [Source:ZFIN;Acc:ZDB-GENE-050809-39])

HSP 1 Score: 303.138 bits (775), Expect = 8.297e-90
Identity = 153/338 (45.27%), Postives = 216/338 (63.91%), Query Frame = 1
Query: 1960 GMDSLQIYPISQASANQRAGRAGRTGPGICYRLYTINQYENDMLPMTVPEIQRTNLTNVVLLLKSLNVQDLMKFYFMDSPPQDNILNSMYHLWMLGALDAVGNLTELGRTMSEFPLDPSLSKMLITSVKHGCSEEMLTIVSMLSVPAIFSRPTGHQEESDCAHEKFQVAESDHLTLLNVYRLWRESGYSANFCKKYYIHNKSMKRVREVRQQLKEIMIKLKMSLASSGDFDM-IRYCICSAYFNQAARWKGLGEYVNLRSGVVCHLHPTSSLYNMAFNADYVVYHELLMTSKEFMNYVTFVDARWLAELGHMFYSVKEKGQSVTDRMLKEQQTVERME 2970
            G+D L + PISQA A QRAGRAGRTGPG  YRLYT   Y ++ML   VPEIQRTNL + VL LK++ + DL+ F FMD+PP + ++ +M  L+ LGALD  G LT LGR M+EFPL+P L KMLI SV  GCSEEMLTIVSMLSV  +F RP   Q  +D    KF  AE DHLTLL VY  W+ + +S  +C + ++  ++++R ++VR+Q+  IM + K+ + S     + ++  + S +F  AA+      Y  L    V ++HP+S+L+N     ++VVYHEL++T+KE+M  VT +D +WL E    F+ V +  +      L +Q+  +R+E
Sbjct:  407 GIDQLVVTPISQAQAKQRAGRAGRTGPGKTYRLYTERAYRDEMLTTNVPEIQRTNLASTVLSLKAMGINDLLSFDFMDAPPMETLITAMEQLYQLGALDDEGLLTRLGRRMAEFPLEPMLCKMLIMSVHLGCSEEMLTIVSMLSVQNVFYRPKDKQALADQKKAKFNQAEGDHLTLLAVYNSWKNNKFSNAWCYENFVQARTLRRAQDVRKQMLGIMDRHKLDVVSCAKATVRVQKAVLSGFFRNAAKKDPQEGYRTLVDQQVVYIHPSSALFNR--QPEWVVYHELVLTTKEYMREVTTIDPKWLVEFAPAFFKVSDPTR------LSKQKKQQRLE 736          

HSP 2 Score: 293.123 bits (749), Expect = 3.649e-86
Identity = 167/350 (47.71%), Postives = 238/350 (68.00%), Query Frame = 3
Query: 1032 DKIALDSEQKSKIDDQRKRLPIFKVRRDLIRLIRENQVIVIVGETGSGKTTQLTQYLLEENYHHKGIIGCTQPXXXXXXXXXXXXXDEVGTKLGDKVGYSIRFEDCKSENTMIKYMTDGIXXXXXXXXXXXXAYSVIIMDEAHERTLNTDILFGLLREIIQRRSDLHLVVTSATMDAEKFSNFFGKCEIFKIPGRTFPVDVIYEKSSILDPVAAAVNKAVDTHLGADSKDGDILVFMSGQEDVEVTCDKIDETLEKVKDTNP--PDLKVEPLYSQLSSERQSEIFKINTSGIRRCIVATNIAETSVTLDGI----------------RNGLVADISHQSSQC*SKSRAGR 2027
            +K +   +    I +QR+ LPI+K++  L++ + +NQ+++++GETGSGKTTQ+TQYL E  Y  +G IGCTQPRRVAA+SVAKRV++E G  LG +VGY+IRFEDC S  T IKYMTDG+LLRE L D +L  YSV+++DEAHERT++TD+LFGLL++ I+RR D+ L+VTSAT+DA KFS +F +  IF IPGRT+PV+++Y K    D + A++   +  HL      GDIL+F++GQE+++  C+ +    E++K   P  P+L + P+YS L SE Q+ IF     G R+ ++ATNIAETS+T+DGI                + G+   +    SQ  +K RAGR
Sbjct:   83 NKASYGKKTAMSIIEQRESLPIYKLKEQLVQAVNDNQILIVIGETGSGKTTQITQYLAEAGYTTRGKIGCTQPRRVAAMSVAKRVSEEFGCCLGQEVGYTIRFEDCTSPETKIKYMTDGMLLRECLIDSELMQYSVVMLDEAHERTIHTDVLFGLLKKTIKRRLDMKLIVTSATLDAVKFSQYFYEAPIFTIPGRTYPVEILYTKEPETDYLDASLITVMQIHL--TEPPGDILLFLTGQEEIDTACEIL---YERMKSLGPEVPELIILPVYSALPSEMQTRIFDPAPPGSRKVVIATNIAETSLTIDGIYYVVDPGFVKQKVYNSKTGIDQLVVTPISQAQAKQRAGR 427          
BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. Ensembl Sea Lamprey
Match: dhx34 (DEAH (Asp-Glu-Ala-His) box polypeptide 34 [Source:ZFIN;Acc:ZDB-GENE-061207-30])

HSP 1 Score: 181.8 bits (460), Expect = 8.932e-85
Identity = 126/323 (39.01%), Postives = 199/323 (61.61%), Query Frame = 3
Query: 1062 SKIDDQRKRLPIFKVRRDLIRLIRENQVIVIVGETGSGKTTQLTQYLLEENYHHKGIIGCTQPXXXXXXXXXXXXXDEVGTKLGDKVGYSIRFEDCKSENTMIKYMTDGIXXXXXXXXXXXXAYSVIIMDEAHERTLNTDILFGLLREIIQRRSDLHLVVTSATMDAEKFSNFFGKCEIFKIPGRTFPVDVIYE---------KSSILDPVA-AAVNKAVDTHLGADSKDGDILVFMSGQEDVEVTCDKIDETLEKVKDTNPPDLKVEPLYSQLSSERQSEIFKINTSGIRRCIVATNIAETSVTLDGIR----NGLVADISH 1988
            +K+   ++ LPI + R  L+ L+R +QV+V+ G+TG GK+TQ+ QYLL         + CTQPRR+A +S+AKRV+ E   + G ++GY IRFE  K+  T + ++T+G+LLR+   +  +  Y V+I+DE HER L+ D+L G+LRE++  R DL LV+ SAT++ + FS + G   + ++PGR +P+ VIY          KS  +DP     + +++D    AD + GD+LVF+SG  D+    +       + K        V  L+S LS+E+Q ++F +   G+R+CIV+TNIAETSVT+DG+R    +G V ++S+
Sbjct:   96 AKLRRDQRNLPIHEYRDQLVELVRAHQVVVVAGDTGCGKSTQVPQYLLSAGMSR---VACTQPRRIACMSLAKRVSYESLGEHGSQIGYQIRFETSKTAATKVLFLTEGLLLRQMQTEGFMSRYDVLILDEVHERHLHCDLLLGVLRELLPLRDDLRLVLMSATINIQLFSRYLGDAPVLQVPGRLYPIQVIYRPVPEEEKTGKSERIDPRPYLRIMQSIDHKYPADER-GDLLVFLSGMADIARVAEAAQSYAVETK-----RWIVLQLHSTLSAEQQDKVFDLAPEGVRKCIVSTNIAETSVTIDGVRFIVDSGKVKEMSY 409          

HSP 2 Score: 151.369 bits (381), Expect = 8.932e-85
Identity = 88/229 (38.43%), Postives = 133/229 (58.08%), Query Frame = 1
Query: 1963 MDSLQIYPISQASANQRAGRAGRTGPGICYRLYTINQYENDMLPMTVPEIQRTNLTNVVLLLKSLNVQDLMKFYFMDSPPQDNILNSMYHLWMLGALDAVGNLTELGRTMSEFPLDPSLSKMLITSVKHGCSEEMLTIVSMLSVPAIFSRPTGHQEESDCAHEKFQVAESDH---LTLLNVYRLWRE--SG---YSANFCKKYYIHNKSMKRVREVRQQLKEIMIKLKM 2625
            M  LQ + IS+ASA QR GRAGRTGPG+C+RLY  ++Y+  + P  VPE+QR  L +VVL +KS+ + D   F F++ PP  ++  SM  L   GALD    LT +GR ++  PLD    KML+     G  E +LTI + +SV + F R        DC+  +  + ESDH   LTLLNV+  W +  SG    S  +CK++ +  + +  +  +++Q KE++++  +
Sbjct:  415 MQRLQEFWISRASAEQRKGRAGRTGPGVCFRLYAESEYDA-LEPYAVPEVQRVPLDSVVLQMKSMGLGDPRLFGFLEPPPASSLETSMQFLQEQGALDEQEELTPIGRVLATLPLDVLTGKMLLVGCVLGAVEPVLTIAAAMSVQSPFHR--NAHTNPDCSRARRSL-ESDHGDPLTLLNVFNEWVQVKSGGQVPSRKWCKRHGLEEQRLYEIANLQRQFKELLVQFGL 639          
BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. Ensembl Sea Lamprey
Match: zgc:158828 (DEAH-box helicase 35 [Source:HGNC Symbol;Acc:HGNC:15861])

HSP 1 Score: 167.548 bits (423), Expect = 3.502e-76
Identity = 97/328 (29.57%), Postives = 180/328 (54.88%), Query Frame = 1
Query: 1960 GMDSLQIYPISQASANQRAGRAGRTGPGICYRLYTINQYENDMLP-MTVPEIQRTNLTNVVLLLKSLNVQDLMKFYFMDSPPQDNILNSMYHLWMLG-ALDAVGNLTELGRTMSEFPLDPSLSKMLITSVKHGCSEEMLTIVSMLSVPAIFSRPTGHQEESDCAHEKFQVAESDHLTLLNVYRLWRESGYSANFCKKYYIHNKSMKRVREVRQQLKEIMIKLKMSLASSGDFD----MIRYCICSAYFNQAARWKGLGEYVNLRSGVVCHLHPTSSL------YNMAFNAD---YVVYHELLMTSKEFMNYVTFVDARWLAELGHMFY 2898
             +D+L + P S+ASA QRAGRAGR+ PG CYRLY  +++    LP  T PE++R+++  V+L LK+L + ++++F F+  PP ++++ ++  L+ LG A     + + LG  M+EFP+ P  +KML+ S   GCS+E++TI +M+ +  +F  P   + ++     KF V E DH+T+LNV+  + +   S+ +C++++++ K + R   ++ QL++++ +  +   SS   D    ++ +CI +     +A+  G G     +S      HP  +L      Y    N      +++  +  T++   +   +    WL EL   F+
Sbjct:  360 AVDALLVTPASRASAQQRAGRAGRSRPGKCYRLYPESEFAK--LPEATEPEMRRSDMAPVILQLKALGIDNVLRFSFLSPPPAESMVQAVELLYALGGAWRDASSSSPLGVRMAEFPMGPMYAKMLLESGTFGCSKEIITIAAMMQIQNVFLSPPNQKIKAARQQRKFAVEEGDHMTMLNVFEAFVKHNKSSAWCQQHFLNYKGLVRAGALQDQLRKLLRRFHIPSNSSNGTDPKLPLLTWCILT-----SAKATGFG--TATKSNTANPPHPLCTLPALIGTYQQHLNLQKIFSIIHQRICETTR---SATIYSGPAWLTELAPHFF 675          

HSP 2 Score: 137.117 bits (344), Expect = 3.502e-76
Identity = 111/307 (36.16%), Postives = 170/307 (55.37%), Query Frame = 3
Query: 1068 IDDQRKRLPIFKVRRDLIRLIRENQVIVIVGETGSGKTTQLTQYLLEENYHHKG-IIGCTQPXXXXXXXXXXXXXDEVGTKLGDKVGYSIRFEDCK-SENTMIKYMTDGIXXXXXXXXXXXXAYSVIIMDEAHERTLNTDILFGLLREIIQRRSDLHLVVTSATMDAEKFSNFFGKCEIFKIPGRTFPVDVIYEKSS--ILDPVAAAVNKAVDT--HLGADSKDGDILVFMSGQEDVE----VTCDKIDETLEKVKDTNPPDLKVEPLYSQLSSERQSEIFKINTSGIRRCIVATNIAETSVTLDGI 1958
            I  QR+RLPIF  R  ++ L+ + + +VIVGETG GK+TQ+ QYL E  +  +G ++  TQPRRVAA +VA RVA+E G  LG +VGY IRF+DC   + T IK++TDG+L+RE + D  L  YS +++                 R +++        VTS+T+ +  FS  F +C+        + V       S  ++ PV   V   V+T   +  D+ DGDIL F++GQE+VE       +++     + K +    ++  P+Y+ L    Q ++F+      R+ +VATN+AETSVT+ GI
Sbjct:   45 IQQQRQRLPIFMHRNHVLYLVEKYRTVVIVGETGCGKSTQIPQYLAEAGWTAEGHVVAITQPRRVAATTVAARVAEERGALLGHEVGYCIRFDDCSDPQATRIKFLTDGMLVREMMADPLLKKYSPLLLRFGDTTAPYPTACSSFNRSLVE--------VTSSTIPSS-FSRAF-ECQCCLADYILWRVGQCTNARSYILMYPVPDYVKTTVETIMKIHNDAADGDILAFLTGQEEVENVVSAVVEEVRAAAARSKGSLHRHVRALPMYAGLPYVDQMKVFERVGRNCRKVVVATNVAETSVTVPGI 341          
BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. Ensembl Sea Lamprey
Match: dqx1 (DEAQ box RNA-dependent ATPase 1 [Source:ZFIN;Acc:ZDB-GENE-150224-1])

HSP 1 Score: 125.176 bits (313), Expect = 2.118e-55
Identity = 94/305 (30.82%), Postives = 159/305 (52.13%), Query Frame = 3
Query: 1080 RKRLPIFKVRRDLIRLIRENQVIVIVGETGSGKTTQLTQYLLEENYHHK---GIIGCTQPXXXXXXXXXXXXXDEVGTKLGDKVGYSIRFEDCKSENTMIKYMTDGIXXXXXXXXXXXXAYSVIIMDEAHERTLNTDILFGLLREIIQRRSDLHLVVTSATMDAEKFSNFFGKCEIFKIPGRTFPVDVIYEKSSILDPVAAAVNKAVDTHLGADSKDGDILVFMSGQEDVEVTCDKIDETLEKVKDTNP--------PDLKVEPLYSQLSSERQSEIFKINTSGIRRCIVATNIAETSVTLDGIR 1961
            R+ LP++  R      I    VI++ GE G GK+TQ+ Q+  E     +   G + C QP  +AA S+A RVADE+   +G +VGYS+ FEDC + +T++++ TD +LLRE   D  LD Y  I++DE  ER+L+TD+L GLLR++ ++R  L +V+ +    AE    ++G     ++ G+   +     ++   D V AAV   ++ H  A  + GD+LVF+   +D        ++   + +  +P        P L   P  +    +           G RR ++++ IAE++ + +G+R
Sbjct:   58 RRALPVWGAREMFRERIASYPVILVSGEPGVGKSTQIPQWCAEFALSRECEHGTVVCAQPHAMAATSLAVRVADEMDLNVGHEVGYSVPFEDCCTTDTILRFTTDEMLLRELTSDPQLDRYGAIVLDEVQERSLSTDVLMGLLRDVGRQRPGLRVVLVATPCAAEALHRYYGDVPWREVVGQQGQLVYTGVETEQRDVVRAAVKLVLEIHRAA--QPGDVLVFLPSDKDASTA---YEQARREARQLSPALGELRLVPLLPGHPGGAGALYDAGGAAATAKGRGGRRVVLSSGIAESAFSFEGVR 357          

HSP 2 Score: 109.768 bits (273), Expect = 2.118e-55
Identity = 61/144 (42.36%), Postives = 89/144 (61.81%), Query Frame = 1
Query: 1966 DSLQIYPISQASANQRAGRAGRTGP-GICYRLYTINQYENDMLPMTVPEIQRTNLTNVVLLLKSLNVQDLMKFYFMDSPPQDNILNSMYHLWMLGALDAVGNLTELGRTMSEFPLDPSLSKMLITSVKHGCSEEMLTIVSMLSV 2394
            DS  + PIS++ A Q +  A R GP G C+RLY  + ++ +M  +  P +  +NLT +VLLLK L++ D+ +  F+D P   N   ++  L  L ALD  GNL+E+G  MSE PLDP L+K L+ S +  C  EMLT+ +ML+V
Sbjct:  377 DSQVVRPISRSQAEQTSCCAVRAGPAGKCFRLYPESVFK-EMPELPPPRVLESNLTRLVLLLKRLDIADMGQCDFVDRPAPANRTKALEDLDYLAALDNDGNLSEVGIIMSEIPLDPQLAKALLASCEFDCVTEMLTLAAMLTV 519          
BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. Ensembl Sea Lamprey
Match: dhx33 (DEAH (Asp-Glu-Ala-His) box polypeptide 33 [Source:NCBI gene;Acc:796505])

HSP 1 Score: 169.474 bits (428), Expect = 1.139e-45
Identity = 123/373 (32.98%), Postives = 197/373 (52.82%), Query Frame = 3
Query: 1062 SKIDDQRKRLPIFKVRRDLIRLIRE-NQVIVIVGETGSGKTTQLTQYLLEENYHHKGIIGCTQPXXXXXXXXXXXXXDEVGTKLGDKVGYSIRFEDCKSENTMIKYMTDGIXXXXXXXXXXXXAYSVIIMDEAHERTLNTDILFGLLREIIQRRSD-----LHLVVTSATMDAEKFSNFFGKCEIFKIPGRTFPVDVIYEKSSILDPVAAAVNKAVDTHLGADSKDGDILVFMSGQEDVEVTCDKIDETLEKVKDTNPPDLKVEPLYSQLSSERQSEIF-KINTSGIRRCIVATNIAETSVTLDGIR----------------NGLVADISHQSSQC*SKSRAGRE-----NWAWHLLPAIHDQSIRERHAPD 2096
            S ++ QR+ LPI+ VR +L R ++E +  IVI G T  G TTQ+  +  E++    G +   QP    AV V  R       +  D VGY++RFED +S +T ++++TDG+LLRE+L D  L A S +++DEAHER+L+TD LFG+++   ++R +     L ++V SATMD ++F ++F    +  + GR  P+ + Y +    D + A +     TH   +++   IL F++GQE++E    +    L +    + P + V PLY+ L   +Q  +F +    G R+ I+ATNIAETS+T+ G+R                 G+      + SQ  +  RAGR           L   +H QS+ E   P+
Sbjct:   32 SGLEVQRRGLPIYAVRAELTRRLQELHTAIVIFGITAQGSTTQVKSWTGEQDCPPGGEVAYMQPSHTTAVCVPSRRDFYRQNRGRDVVGYTVRFEDVRSASTRVRFVTDGMLLREALGDPLLLATSAVVLDEAHERSLHTDTLFGVVKGAQRQRWEQKLRPLKVIVMSATMDVDRFVSYFNGAPVLYVEGRQHPIQIFYTREPHSDFLHATLITIFQTHQEQEAEH-HILAFLTGQEEIEAM-GRACRDLSRQLPPSVPRMCVVPLYAALPHAQQQRVFARAPPKGTRKVILATNIAETSITIPGVRTVIDCGLVKAKMFSPGTGMEVLALQKVSQAQAWQRAGRSGREGPGCCLRLYTEMHFQSLAENTTPE 402          

HSP 2 Score: 80.1073 bits (196), Expect = 7.228e-16
Identity = 42/85 (49.41%), Postives = 56/85 (65.88%), Query Frame = 1
Query: 1948 STGYGMDSLQIYPISQASANQRAGRAGRTGPGICYRLYTINQYENDMLPMTVPEIQ-RTNLTNVVLLLKSLNVQDLMKFYFMDSP 2199
            S G GM+ L +  +SQA A QRAGR+GR GPG C RLYT   +++ +   T PE+Q R NLT+V+L L +L + D+  F FMD P
Sbjct:  350 SPGTGMEVLALQKVSQAQAWQRAGRSGREGPGCCLRLYTEMHFQS-LAENTTPELQSRCNLTSVLLHLLALGISDVASFDFMDKP 433          
BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. Ensembl Yeast
Match: PRP16 (DEAH-box RNA helicase involved in second catalytic step of splicing and in exon ligation; exhibits ATP-dependent RNA unwinding activity; mediates the release of Yju2p and Cwc25p in the second step; in the absence of ATP, stabilizes the binding of Cwc25p to the spliceosome in the first catalytic step; missense mutation in human ortholog DHX38 associated with early-onset retinitis pigmentosa [Source:SGD;Acc:S000001794])

HSP 1 Score: 345.895 bits (886), Expect = 7.773e-179
Identity = 179/350 (51.14%), Postives = 250/350 (71.43%), Query Frame = 1
Query: 1960 GMDSLQIYPISQASANQRAGRAGRTGPGICYRLYTINQYENDMLPMTVPEIQRTNLTNVVLLLKSLNVQD-LMKFYFMDSPPQDNILNSMYHLWMLGALDAVGNLTELGRTMSEFPLDPSLSKMLITSVKHGCSEEMLTIVSMLSVPAIFSRPTGHQEESDCAHEKFQVAESDHLTLLNVYRLWRESGYSANFCKKYYIHNKSMKRVREVRQQLKEIMIKLKMSLASSG-DFDMIRYCICSAYFNQAARWKGLGEYVNLRSGVVCHLHPTSSLYNMAFNADYVVYHELLMTSKEFMNYVTFVDARWLAELGHMFYSVKE---KGQSVTDRMLKE--QQTVERMETEMRIN 2988
            G+DSL I PIS+A+A+QR+GRAGRT PG  YRLYT + ++ DM   T+PEIQRTNL+N +LLLKSL+V D L KF F+D PP    L+S+Y LW +GA+D  G LT LG  M++FPL PSLSK+L+ +V++GCS+EMLTIVSMLSVP +F RP   Q+E+D A  KF +A+SDHLTLLNV+  WR + +S+++C K+++  KS+ R R++R QL  I+   K+ + SSG D+D+I+ CICS + +QAA+  GL  YV+L++GV   LHPTS+L+ +     YVVYHELLMTSKE++  VT VD  WL E G + Y +K      ++ T  +  E  + T++++E ++ IN
Sbjct:  669 GLDSLVITPISKANADQRSGRAGRTAPGTAYRLYTEDTFKEDMYLQTIPEIQRTNLSNTLLLLKSLDVTDELSKFPFIDKPPLQTFLSSLYELWFIGAIDTSGQLTPLGLQMAKFPLQPSLSKILLIAVRNGCSDEMLTIVSMLSVPQVFYRPKERQKEADIARNKFFIAKSDHLTLLNVFEQWRANNFSSHWCNKHFVQYKSLVRARDIRDQLLTILKSQKIPVISSGKDWDIIKKCICSGFAHQAAKITGLRNYVHLKTGVSVQLHPTSALHGLGDLPPYVVYHELLMTSKEYICCVTSVDPFWLMEYGGLLYDIKRIKNDQEATTTGLFGEHYEHTLDKVEDDIDIN 1018          

HSP 2 Score: 300.056 bits (767), Expect = 7.773e-179
Identity = 227/555 (40.90%), Postives = 308/555 (55.50%), Query Frame = 3
Query:  417 RNDKSEIK-WSPDKNEE-ELDREWYLNDGMEYNPFPNFSDEVLEKKKQQLQRQTSIVAKSRKSARAKQFEETNRAWEESKLINSGAVVRKGFNVEIDNEDTGEDRIHLHVHNI------LPPFLD--------------GRIVFTKQ----FEPVIPVKDENSDMAKIAKNGSQMVKNYRDIKEKMKLQKRTLDMTGTNFGKLMGLEKKETLDKWDETNYKDSMKYCDKITDKIALDSEQKSKIDDQRKRLPIFKVRRDLIRLIRENQVIVIVGETGSGKTTQLTQYLLEENYHH---KGIIGCTQPXXXXXXXXXXXXXDEVGTKLGDKVGYSIRFEDCK-SENTMIKYMTDGIXXXXXXXXXXXXAYSVIIMDEAHERTLNTDILFGLLREIIQRRSDLHLVVTSATMDAEKFSNFFGKCEIFKIPGRTFPVDVIYEKSSILDPVAAAVNKAVDTHLGADSKDGDILVFMSGQEDVEVTCDKIDETLEKV----------KDTNPPDLKVEPLYSQLSSERQSEIFKINTSGIRRCIVATNIAETSVTLDGIR 1961
            +N  +E K + P ++E  E DREWY ND    N  P                                 E  +   EE+KL+    V+R      IDN+D   + + L+   +      +PPFL               G I  T         V P ++ +S+ +  AK GS++V   R   E ++  +    +  T  G+++GLE           N K   K   KI D  AL +  K  I   +++LP+F+ R  L+ LIRENQV+VI+GETGSGKTTQL QYL EE Y +   K I+  TQPRRVAA+SVAKRVA E+   LG +VGYSIRFED   SE T +K++TDGILLRE+L D+ LD YS +I+DEAHER+LNTDIL G  + ++ RR DL L++TSATM+A+KFS FFG    F IPGRTFPV  IY  + + D V AAV++AV  HL  D   GDIL+FM+GQED+E T D + E   +V          ++ N  D+++ P+YS L ++ Q +IF+      R+ I+ATNIAETS+T+ GIR
Sbjct:  151 QNGHAETKDYEPTRSEVVEEDREWYDNDDDYGNLVP---------------------------------EPLSELPEEAKLL---PVIR-----NIDNDDALRNTVQLYPIPLKQRMEWIPPFLSKFALENKVPTSIIIGSISETSSQVSALSMVNPFRNPDSEFSANAKRGSKLVALRRINMEHIQQSRDNTTVLNTAMGEVLGLE----------NNNKAKDKSNQKICDDTALFTPSKDDIKHTKEQLPVFRCRSQLLSLIRENQVVVIIGETGSGKTTQLAQYLYEEGYANDRGKSIV-VTQPRRVAAISVAKRVAMEMQVPLGKEVGYSIRFEDVTDSECTKLKFVTDGILLRETLLDDTLDKYSCVIIDEAHERSLNTDILLGFFKILLARRRDLKLIITSATMNAKKFSAFFGNAPQFTIPGRTFPVQTIYTSNPVQDYVEAAVSQAVKIHLANDCSSGDILIFMTGQEDIETTFDTLQEKFLQVYSKKFGTANFEEIN--DIEILPIYSALPADLQFKIFQDLHGTKRKIIIATNIAETSLTIKGIR 651          
BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. Ensembl Yeast
Match: PRP22 (DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase; associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes; required for proofreading the exon ligation reaction [Source:SGD;Acc:S000000815])

HSP 1 Score: 305.834 bits (782), Expect = 3.391e-164
Identity = 145/314 (46.18%), Postives = 211/314 (67.20%), Query Frame = 1
Query: 1960 GMDSLQIYPISQASANQRAGRAGRTGPGICYRLYTINQYENDMLPMTVPEIQRTNLTNVVLLLKSLNVQDLMKFYFMDSPPQDNILNSMYHLWMLGALDAVGNLTELGRTMSEFPLDPSLSKMLITSVKHGCSEEMLTIVSMLSVPAIFSRPTGHQEESDCAHEKFQVAESDHLTLLNVYRLWRESGYSANFCKKYYIHNKSMKRVREVRQQLKEIMIKLKMSLAS-SGDFDMIRYCICSAYFNQAARWKGLGEYVNLRSGVVCHLHPTSSLYNMAFNADYVVYHELLMTSKEFMNYVTFVDARWLAELGHMFY 2898
            G++ L + PISQA ANQR GRAGRTGPG CYRLYT + + N+ML  TVPEIQR NL++ +L+LK++ + DL+KF FMD PP++ +LN++  L+ L +LD  G LT LG+ MS FP+DP+LS+ L++SV + CS+E++TI+SMLSV  +F RP   Q E+D    KF     DHLTLLNVY  W+++ YS  +CK  ++H + +KR R+V+ Q+  I  K+ + L S   D D+IR    S +F  AA+      Y  +  G    +HP+SSLY   +  +YV+YH +++TS+E+M+ VT ++ +WL E+   FY
Sbjct:  788 GIEQLIVSPISQAQANQRKGRAGRTGPGKCYRLYTESAFYNEMLENTVPEIQRQNLSHTILMLKAMGINDLLKFDFMDPPPKNLMLNALTELYHLQSLDDEGKLTNLGKEMSLFPMDPTLSRSLLSSVDNQCSDEIVTIISMLSVQNVFYRPKDRQLEADSKKAKFHHPYGDHLTLLNVYTRWQQANYSEQYCKTNFLHFRHLKRARDVKSQISMIFKKIGLKLISCHSDPDLIRKTFVSGFFMNAAKRDSQVGYKTINGGTEVGIHPSSSLYGKEY--EYVMYHSIVLTSREYMSQVTSIEPQWLLEVAPHFY 1099          

HSP 2 Score: 291.582 bits (745), Expect = 3.391e-164
Identity = 177/414 (42.75%), Postives = 275/414 (66.43%), Query Frame = 3
Query:  753 PPFL-DGRIVFTKQFEPVIPVKDENSDMAKIAKNGSQMVKNYRD--------IKEKMKLQKRTLDMTGT---NFGKLMGLEKKETLDKWDETNYKDSMKYCDKITDKIALDSEQKSKIDDQRKRLPIFKVRRDLIRLIRENQVIVIVGETGSGKTTQLTQYLLEENYHHKGIIGCTQPXXXXXXXXXXXXXDEVGTKLGDKVGYSIRFEDCKSENTMIKYMTDGIXXXXXXXXXXXXAYSVIIMDEAHERTLNTDILFGLLREIIQRRSDLHLVVTSATMDAEKFSNFFGKCEIFKIPGRTFPVDVIYEKSSILDPVAAAVNKAVDTHLGADSKDGDILVFMSGQEDVEVTCDKIDETLEKVKDTNPPDLKVEPLYSQLSSERQSEIFKINTSGIRRCIVATNIAETSVTLDGI 1958
            P FL D ++   K++E     K     M + A NGS  ++++R+        I+++++ Q+   D T     +  ++  L+ +  + +W++    +S+ Y  + +  I+           QR+ LP++ +R +LI+ +R+NQ +VIVGETGSGKTTQ+TQYL EE + + G+IGCTQPRRVAAVSVAKRVA+EVG K+G  VGY+IRFED    +T IKYMTDG+L RE+L D ++  YSVI++DEAHERT+ TD+LF LL++   +R +L ++VTSAT+++ KFS +F  C I  IPG+TFPV+V+Y ++  +D + AA++  +D H+  +   GDILVF++GQE+++  C+ + + ++ + D+   +L + P+YS L SE QS+IF+    G R+ + ATNIAETS+T+DGI
Sbjct:  369 PKFLKDQQVKGAKKYEMPKITKVPRGFMNRSAINGSNAIRDHREEKLRKKREIEQQIRKQQSFDDPTKNKKDSRNEIQMLKNQLIVTEWEKNRMNESISYGKRTSLPISA----------QRQTLPVYAMRSELIQAVRDNQFLVIVGETGSGKTTQITQYLDEEGFSNYGMIGCTQPRRVAAVSVAKRVAEEVGCKVGHDVGYTIRFEDVTGPDTRIKYMTDGMLQREALLDPEMSKYSVIMLDEAHERTVATDVLFALLKKAAIKRPELKVIVTSATLNSAKFSEYFLNCPIINIPGKTFPVEVLYSQTPQMDYIEAALDCVIDIHI--NEGPGDILVFLTGQEEIDSCCEILYDRVKTLGDS-IGELLILPVYSALPSEIQSKIFEPTPKGSRKVVFATNIAETSITIDGI 769          
BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. Ensembl Yeast
Match: PRP43 (RNA helicase in the DEAH-box family; functions in both RNA polymerase I and polymerase II transcript metabolism; catalyzes removal of U2, U5, and U6 snRNPs from the postsplicing lariat-intron ribonucleoprotein complex; required for efficient biogenesis of both small- and large-subunit rRNAs; acts with Sqs1p to promote 20S to 18S rRNA processing catalyzed by endonuclease Nob1p [Source:SGD;Acc:S000003088])

HSP 1 Score: 244.973 bits (624), Expect = 1.511e-128
Identity = 128/324 (39.51%), Postives = 198/324 (61.11%), Query Frame = 1
Query: 1963 MDSLQIYPISQASANQRAGRAGRTGPGICYRLYTINQYENDMLPMTVPEIQRTNLTNVVLLLKSLNVQDLMKFYFMDSPPQDNILNSMYHLWMLGALDAVGNLTELGRTMSEFPLDPSLSKMLITSVKHGCSEEMLTIVSMLSVPAIFSRPTGHQEESDCAHEKFQVAESDHLTLLNVYRLWR-ESGYS---ANFCKKYYIHNKSMKRVREVRQQLKEIMIKLKMSL-----ASSGDFDMIRYCICSAYFNQAARWK-GLGEYVNLRSGVVCHLHPTSSLYNMAFNADYVVYHELLMTSKEFMNYVTFVDARWLAELGHMFYSV 2904
            ++SL + PIS+ASA QRAGRAGRT PG C+RLYT   ++ +++  + PEI R+NL++ VL LK L + DL+ F FMD P  + ++ ++  L  L  LD  GNLT LGR  S+FPLDP L+ MLI S +  CS+E+LTIV+MLSVP +F RPT  ++ +D A   F   + DH+TLLNVY  ++ +  Y      +C+ +Y++ +S+     +R QL+ +M +  + L      S   FD IR  + S +F Q A+ + G   Y+ ++      +HP++ L     +A++V+Y+E ++TSK ++  VT V   WL E+   +Y +
Sbjct:  408 VESLLVSPISKASAQQRAGRAGRTRPGKCFRLYTEEAFQKELIEQSYPEILRSNLSSTVLELKKLGIDDLVHFDFMDPPAPETMMRALEELNYLACLDDEGNLTPLGRLASQFPLDPMLAVMLIGSFEFQCSQEILTIVAMLSVPNVFIRPTKDKKRADDAKNIFAHPDGDHITLLNVYHAFKSDEAYEYGIHKWCRDHYLNYRSLSAADNIRSQLERLMNRYNLELNTTDYESPKYFDNIRKALASGFFMQVAKKRSGAKGYITVKDNQDVLIHPSTVL---GHDAEWVIYNEFVLTSKNYIRTVTSVRPEWLIEIAPAYYDL 728          

HSP 2 Score: 233.417 bits (594), Expect = 1.511e-128
Identity = 140/301 (46.51%), Postives = 204/301 (67.77%), Query Frame = 3
Query: 1080 RKRLPIFKVRRDLIRLIRENQVIVIVGETGSGKTTQLTQYLLEENYHH--KGIIGCTQPXXXXXXXXXXXXXDEVGTKLGDKVGYSIRFEDCKSENTMIKYMTDGIXXXXXXXXXXXXAYSVIIMDEAHERTLNTDILFGLLREIIQRRSDLHLVVTSATMDAEKFSNFFGKCEIFKIPGRTFPVDVIYEKSSILDPVAAAVNKAVDTHLGADSKDGDILVFMSGQEDVEVTCDKID-ETLEKVKDTNPPDLKVEPLYSQLSSERQSEIFKI-----NTSGIRRCIVATNIAETSVTLDGI 1958
            R+ LP+   R + ++L + NQ++V VGETGSGKTTQ+ Q++L +   H     + CTQPRRVAA+SVA+RVA+E+  KLG++VGYSIRFE+  S  T++KYMTDG+LLRE++ D DL  YS II+DEAHERTL TDIL GLL+++++RR DL +++ SAT+DAEKF  +F    +  +PGRT+PV++ Y      D + +A+   +  H  A  + GDIL+F++G++++E    KI  E  + V++     L V PLY  L   +Q  IF+      N    R+ +++TNIAETS+T+DGI
Sbjct:   90 RRELPVHAQRDEFLKLYQNNQIMVFVGETGSGKTTQIPQFVLFDEMPHLENTQVACTQPRRVAAMSVAQRVAEEMDVKLGEEVGYSIRFENKTSNKTILKYMTDGMLLREAMEDHDLSRYSCIILDEAHERTLATDILMGLLKQVVKRRPDLKIIIMSATLDAEKFQRYFNDAPLLAVPGRTYPVELYYTPEFQRDYLDSAIRTVLQIH--ATEEAGDILLFLTGEDEIEDAVRKISLEGDQLVREEGCGPLSVYPLYGSLPPHQQQRIFEPAPESHNGRPGRKVVISTNIAETSLTIDGI 388          
BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. Ensembl Yeast
Match: PRP2 (RNA-dependent DExD/H-box ATPase; required for activation of spliceosome before first transesterification step in RNA splicing; implicated in rearranging and proofreading snRNA structure in catalytic activation of spliceosome; ortholog of human protein DHX16 [Source:SGD;Acc:S000005294])

HSP 1 Score: 256.529 bits (654), Expect = 1.558e-121
Identity = 154/312 (49.36%), Postives = 217/312 (69.55%), Query Frame = 3
Query: 1041 ALDSEQKS--KIDDQRKRLPIFKVRRDLIRLIRENQVIVIVGETGSGKTTQLTQYLLEENYHHKG--IIGCTQPXXXXXXXXXXXXXDEVGTKLGDKVGYSIRFEDCKSEN-TMIKYMTDGIXXXXXXXXXXXXAYSVIIMDEAHERTLNTDILFGLLREIIQRRSDLHLVVTSATMDAEKFSNFFGKCEIFKIPGRTFPVDVIYEKSSILDPVAAAVNKAVDTHLGADSKDGDILVFMSGQEDVEVTCDKIDETLEKVKDTNPPDLKVEPLYSQLSSERQSEIFKINTSGIRRCIVATNIAETSVTLDGIR 1961
            AL++E+K    I + RK LP+ + + +L++ I++NQV++I+GETGSGKTTQL QYL+E+ +  +G   I  TQPRRVAA SVA RVADE+   LG +VGY IRFED  + N T++KYMTDG+LLRE L D  L  YS I++DEAHERTL TDIL GLL++I+ +R  L L+++SATM+A+KFS FF  C IF +PGR +PVD+ Y      + + AA+      H    S  GDILVF++GQE++E T  K++E + K+  +    + + P+Y+ L  E+Q +IF+      R+ ++ATNIAETS+T+DGIR
Sbjct:  205 ALETEEKRILTIQEARKLLPVHQYKDELLQEIKKNQVLIIMGETGSGKTTQLPQYLVEDGFTDQGKLQIAITQPRRVAATSVAARVADEMNVVLGKEVGYQIRFEDKTTPNKTVLKYMTDGMLLREFLTDSKLSKYSCIMIDEAHERTLATDILIGLLKDILPQRPTLKLLISSATMNAKKFSEFFDNCPIFNVPGRRYPVDIHYTLQPEANYIHAAITTIFQIH-TTQSLPGDILVFLTGQEEIERTKTKLEEIMSKL-GSRTKQMIITPIYANLPQEQQLKIFQPTPENCRKVVLATNIAETSLTIDGIR 514          

HSP 2 Score: 198.749 bits (504), Expect = 1.558e-121
Identity = 125/337 (37.09%), Postives = 181/337 (53.71%), Query Frame = 1
Query: 1945 PSTGYGMDSLQIYPISQASANQRAGRAGRTGPGICYRLYTINQYENDMLPMTVPEIQRTNLTNVVLLLKSLNVQDLMKFYFMDSPPQDNILNSMYHLWMLGALDAVGNLTELGRTMSEFPLDPSLSKMLITSVKH----GCSEEMLTIVSML-SVPAIFSRPTGHQEESDCAHEKFQVAESDHLTLLNVYRLWRESGYSANFCKKYYIHNKSMKRVREVRQQL-----------KEIMIKLKMSLASSGDFDMIRYCICSAYFNQAARWKGLGEYVNLRS--GVVCHLHPTSSLY-----NMAFNADYVVYHELLMTSKEFM-NYVTFVDARWLAEL 2883
            PSTG  M  L   P S+AS +QRAGRAGR GPG C+R++T   Y +++  M  PEI RTNL+N VLLL SL V DL+KF  MD P    +  S+ +L++LGAL++ G +T LG+ M EFP +P  +K+L T+  H    G  EE LTIVSML   P++F       ++ D A       ESDH+  L ++  WR S +S ++C+ + I  K+M RVR +R QL           K    ++K+   +      I  C  S +     +    G     RS  G+   +HPTS L+          + YV+Y +L++TSKEF+ + +      WL ++
Sbjct:  529 PSTG--MTQLLTVPCSRASVDQRAGRAGRVGPGKCFRIFTKWSYLHELELMPKPEITRTNLSNTVLLLLSLGVTDLIKFPLMDKPSIPTLRKSLENLYILGALNSKGTITRLGKMMCEFPCEPEFAKVLYTAATHEQCQGVLEECLTIVSMLHETPSLFI-----GQKRDAAASVLSEVESDHILYLEIFNQWRNSKFSRSWCQDHKIQFKTMLRVRNIRNQLFRCSEKVGLVEKNDQARMKIGNIAGYINARITRCFISGFPMNIVQLGPTGYQTMGRSSGGLNVSVHPTSILFVNHKEKAQRPSKYVLYQQLMLTSKEFIRDCLVIPKEEWLIDM 858          
BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. Ensembl Yeast
Match: DHR2 (Predominantly nucleolar DEAH-box ATP-dependent RNA helicase; required for 18S rRNA synthesis [Source:SGD;Acc:S000001561])

HSP 1 Score: 210.69 bits (535), Expect = 2.018e-88
Identity = 135/308 (43.83%), Postives = 199/308 (64.61%), Query Frame = 3
Query: 1059 KSKIDD---QRKRLPIFKVRRDLIRLIRENQVIVIVGETGSGKTTQLTQYLLEENYHHK--GIIGCTQPXXXXXXXXXXXXXDEVGTKLGDKVGYSIRFEDCKSENTMIKYMTDGIXXXXXXXXXXXXAYSVIIMDEAHERTLNTDILFGLLREIIQR-RSDLHLVVTSATMDAEKFSNFFGKCEIFKIPGRTFPVDVIYEKSSILDPVAAAVNKAVDTHLGADSKDGDILVFMSGQEDVEVTCDKIDETLEKVKDTNPPDLKVE-PLYSQLSSERQSEIFKINTSGIRRCIVATNIAETSVTLDGIR 1961
            KS+  D    R+ LP+++ +R+++  I  N V V++GETGSGK+TQ+ Q++LE+ Y  K  G I  TQPRRVAA+++A RVA E G KLG++VGYS+RF++  +  T +KY+TDG+LLRE + + DL  YSVI++DEAHERT+ TD++ G L+ +IQ  R DL ++V SAT+ AEKFS FF    I  + GR F V   Y K+   D V A +   +  + G +   GDIL F+ GQE+++     +++  + V D  P  L V  PLY+ L + +QS +F       R+ + +TNIAETSVT+ G++
Sbjct:   68 KSRASDLLKMRETLPVYQHKREIMSYIESNPVTVLIGETGSGKSTQIPQFVLEKLYDTKKHGSIAVTQPRRVAAINLATRVAQEHGCKLGEQVGYSVRFDNTTTTRTRLKYLTDGMLLRELMMNSDLREYSVIVIDEAHERTVLTDLILGFLKSLIQGPRPDLRIIVMSATLQAEKFSEFFNNAPILFVEGRKFDVKQYYLKAPTDDIVDAVIRCCIQINQGEEL--GDILCFLPGQEEIDKAVTIMEKIAKYVSDEAPVPLIVPYPLYAALPAVQQSLVFAPIKGFKRKVVFSTNIAETSVTISGVK 373          

HSP 2 Score: 134.42 bits (337), Expect = 2.018e-88
Identity = 102/341 (29.91%), Postives = 164/341 (48.09%), Query Frame = 1
Query: 1960 GMDSLQIYPISQASANQRAGRAGRTGPGICYRLYTINQYENDMLP-MTVPEIQRTNLTNVVLLLKSLNVQDLMKFYFMDSPPQDNILNSMYHLWMLGALDAVGNLTELGRTMSEFPLDPSLSKMLITSVKHGCSEEMLTIVSMLSVPAIFSRPTGHQEESDCAHEKFQVAESDHLTLLNV------------------YRLWRESGYSA---NFCKKYYIHNKSMKRVREVRQQLKEIMIKL----------KMSLASSGDF-DMIRYCICSAYFNQAARWKGLGEYVNLRSGVVCHLHPTSSLYNMAFNADYVVYHELLMTSKEFMNYVTFVDARWLAEL 2883
            G+ +L   PISQASA QR+GRAGR   G  +RLY  + Y    LP  + PEI R+++T+ VL+LK   V DL+ + + ++P ++ I+  +  L+ LGALD  G +T+ G+ M+  PL P LS +LI + + GC  +++ IVS LSV  +   P+   EE D  +E+        L+L N                   Y L +    S+   ++CK   I  +  K V  VR QL+    +L             +   G+    I  C  + +    A       Y  + +G    +HP+S L+ M  +   ++Y E + T+K +   V+ ++  WL E+
Sbjct:  391 GLATLLTVPISQASAMQRSGRAGRESEGKSFRLYCESDYVK--LPKQSEPEIARSDVTSPVLMLKRYGVDDLLNWTWFENPGKEAIVMGLQELYELGALDTRGKITKRGQQMALLPLQPHLSSVLIKASEVGCLSQVIDIVSCLSVENLLLNPS--PEERDEVNER-------RLSLCNAGKRYGDLIMLKELFDIYFYELGKSQDASSERNDWCKGLCISIRGFKNVIRVRDQLRVYCKRLFSSISEEDEESKKIGEDGELISKILKCFLTGFIKNTAIGMPDRSYRTVSTGEPISIHPSSMLF-MNKSCPGIMYTEYVFTTKGYARNVSRIELSWLQEV 719          
BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. Ensembl Nematostella
Match: EDO35622 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SKZ8])

HSP 1 Score: 451.44 bits (1160), Expect = 7.536e-144
Identity = 257/509 (50.49%), Postives = 369/509 (72.50%), Query Frame = 3
Query:  450 DKNEEELDREWY-LNDGME--YNPFPNFSDEVLEKKKQQLQRQTSIVAKSRKSARAKQFEETNRAWEESKLINSGAVVRKGFNVEIDNEDTGEDRIHLHVHNILPPFLDGRIVFTKQFEPVIPVKDENSDMAKIAKNGSQMVKNYRDIKEKMKLQKRTLDMTGTNFGKLMGLEKKETLDKWDETN-YKDSMKYCDKITDKIALDSEQKSK--IDDQRKRLPIFKVRRDLIRLIRENQVIVIVGETGSGKTTQLTQYLLEENYHHKGIIGCTQPXXXXXXXXXXXXXDEVGTKLGDKVGYSIRFEDCKSENTMIKYMTDGIXXXXXXXXXXXXAYSVIIMDEAHERTLNTDILFGLLREIIQRRSDLHLVVTSATMDAEKFSNFFGKCEIFKIPGRTFPVDVIYEKSSILDPVAAAVNKAVDTHLGADSKDGDILVFMSGQEDVEVTCDKIDETLEKVKDTNPPDLKVEPLYSQLSSERQSEIFKINTSGIRRCIVATNIAETSVTLDGI 1958
            ++ ++ LDR WY ++ G +   NPF + S    E+  ++ + +    A  + SA+ +Q  + N  WE ++++ SG V +   +V+ D E+  E ++HL VHNI+PPFLDGRIVFTKQ EPVIP+KD  SDMA I++ G  +V+ +R+ KE+ K Q++  ++ GT  G ++G+++++  +       ++++ K+   + DK    S+  SK  +  QR+ LPIF VR++L+ ++R+NQV++IVGETGSGKTTQLTQY+ E+ Y + G+IGCTQPRRVAA+SVAKRV++E+   LG++VGY+IRFED  S+ TMIKYMTDGILLRESLR+ DLD YSVIIMDEAHER+LNTD+LFGLLR+++ RR DL L+VTSATMDA+KF++FFG    +KIPGRTFPV++ + ++ + D V +AV +A+  HL      GDILVFM GQED+EVTC+ I E L +V++  P  L V P+YSQL S+ Q++IF+    G+R+C+VATNIAETS+T+DGI
Sbjct:  314 EEEQKRLDRAWYDMDTGYDETQNPFADVS----EEYTKKKEEKLIKKAVKKMSAQQRQINKDNDMWETNRMLTSGVVQK--LDVDEDFEEENEAKVHLLVHNIVPPFLDGRIVFTKQPEPVIPLKDPTSDMAIISRKGCAVVRRFREEKERKKAQQKHWELAGTKLGNILGVKEEKGEEGEAVEGEHRNAQKFAKHMKDKSQASSDFASKKSLVQQRQYLPIFAVRQELLSIVRDNQVVIIVGETGSGKTTQLTQYMHEDGYTNYGMIGCTQPRRVAAMSVAKRVSEEMDCSLGEEVGYAIRFEDVTSDKTMIKYMTDGILLRESLRESDLDHYSVIIMDEAHERSLNTDVLFGLLRDVVSRRRDLKLIVTSATMDAQKFADFFGNVPTYKIPGRTFPVEIFFTRTVMEDYVDSAVKQALQIHLTPSK--GDILVFMPGQEDIEVTCELISERLAEVEEAPP--LAVLPIYSQLPSDLQAKIFQKAPDGVRKCVVATNIAETSLTVDGI 812          

HSP 2 Score: 59.3066 bits (142), Expect = 8.414e-9
Identity = 24/28 (85.71%), Postives = 28/28 (100.00%), Query Frame = 1
Query: 1960 GMDSLQIYPISQASANQRAGRAGRTGPG 2043
            GMD+LQ+YPISQA+ANQR+GRAGRTGPG
Sbjct:  831 GMDALQVYPISQANANQRSGRAGRTGPG 858          
BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. Ensembl Nematostella
Match: EDO35621 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SKZ7])

HSP 1 Score: 354.755 bits (909), Expect = 3.487e-114
Identity = 165/265 (62.26%), Postives = 207/265 (78.11%), Query Frame = 1
Query: 2188 MDSPPQDNILNSMYHLWMLGALDAVGNLTELGRTMSEFPLDPSLSKMLITSVKHGCSEEMLTIVSMLSVPAIFSRPTGHQEESDCAHEKFQVAESDHLTLLNVYRLWRESGYSANFCKKYYIHNKSMKRVREVRQQLKEIMIKLKMSLASSG-DFDMIRYCICSAYFNQAARWKGLGEYVNLRSGVVCHLHPTSSLYNMAFNADYVVYHELLMTSKEFMNYVTFVDARWLAELGHMFYSVKEKGQSVTDRMLKEQQTVERMETEM 2979
            MD PPQ+NILNSMY LW+LGALD  G+LT LGR M EFPLDP++SKMLI SV  GCSEE+LTIVSMLSVPAIF RP G +E+SD A EKF V ESDHLT LNVY  W+ + Y A +  +++IH K+M++VREVR QLK+IM++ K+ L S G D+D+IR CICS+YF+QAAR KG+GEYVN+R+G+ CHLHPTS+L+ M +  DY+VYHEL+MTSKE+M  VT VD  WLAELG MFYSVKE  +S  +   + ++ +  ME E+
Sbjct:    1 MDPPPQENILNSMYQLWILGALDNTGSLTPLGRQMVEFPLDPAMSKMLIVSVDMGCSEEVLTIVSMLSVPAIFFRPKGREEDSDAAREKFAVPESDHLTYLNVYLQWKTNNYGAQWSSEHFIHVKAMRKVREVRGQLKDIMVQQKIPLTSCGSDWDVIRKCICSSYFHQAARLKGIGEYVNMRTGMPCHLHPTSALFGMGYTPDYIVYHELVMTSKEYMQCVTAVDGNWLAELGPMFYSVKESTRSRAENRRRAKEEMSAMEREL 265          
BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. Ensembl Nematostella
Match: EDO49560 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RGM4])

HSP 1 Score: 300.442 bits (768), Expect = 4.299e-91
Identity = 151/338 (44.67%), Postives = 216/338 (63.91%), Query Frame = 1
Query: 1960 GMDSLQIYPISQASANQRAGRAGRTGPGICYRLYTINQYENDMLPMTVPEIQRTNLTNVVLLLKSLNVQDLMKFYFMDSPPQDNILNSMYHLWMLGALDAVGNLTELGRTMSEFPLDPSLSKMLITSVKHGCSEEMLTIVSMLSVPAIFSRPTGHQEESDCAHEKFQVAESDHLTLLNVYRLWRESGYSANFCKKYYIHNKSMKRVREVRQQLKEIMIKLKMSLASSGDFDM-IRYCICSAYFNQAARWKGLGEYVNLRSGVVCHLHPTSSLYNMAFNADYVVYHELLMTSKEFMNYVTFVDARWLAELGHMFYSVKEKGQSVTDRMLKEQQTVERME 2970
            GMD+L + PISQA A QR+GRAGRTGPG CYRLYT   Y ++ML   VPEIQRTNL + +L LK++ + DL+ F FMD PP + ++++M  L  L ALD  G LT LGR M+EFPL+P LSKMLI SV  GCS+E+LTIVSMLSV  +F RP   Q  +D    KF   E DHLTLL VY  W+ + +S  +C + ++  +S++R +++R+Q+  +M + K+ + S G     ++  I S +F  AAR      Y  +    V ++HP+S+L+N     ++VVYHEL++T+KE+M  VT +D +WL E    F+ V +  +      L +++  ER+E
Sbjct:  170 GMDALVVTPISQAQAKQRSGRAGRTGPGKCYRLYTERAYRDEMLATAVPEIQRTNLASTILSLKAMGINDLLSFDFMDPPPMETMISAMEALHSLSALDNEGLLTRLGRRMAEFPLEPQLSKMLIQSVHLGCSDEILTIVSMLSVQNVFYRPKDKQALADQKKAKFHQPEGDHLTLLAVYNSWKNNKFSNAWCFENFVQARSLRRAQDIRKQMLGLMDRHKLDVVSCGKHTARVQKAITSGFFRNAARKDPQEGYRTVVDNQVVYIHPSSALFNR--QPEWVVYHELVLTTKEYMREVTTIDPKWLVEFAPSFFRVADPTK------LSKRKRHERLE 499          

HSP 2 Score: 150.214 bits (378), Expect = 2.758e-38
Identity = 82/195 (42.05%), Postives = 119/195 (61.03%), Query Frame = 3
Query: 1497 LREIIQRRSDLHLVVTSATMDAEKFSNFFGKCEIFKIPGRTFPVDVIYEKSSILDPVAAAVNKAVDTHLGADSKDGDILVFMSGQEDVEVTCDKIDETLEKVKDTNP--PDLKVEPLYSQLSSERQSEIFKINTSGIRRCIVATNIAETSVTLDGI----------------RNGLVADISHQSSQC*SKSRAGR 2027
            +++ +QRR DL L+VTSAT+DA KFS FF +  IF IPGRTFPV+++Y K +  D + AA+   +  HL      GDILVF++GQE+++  C+ +    E+ K   P  P+L + P+YS L SE Q+ IF     G R+C++ATNIAETS+T+DGI                ++G+ A +    SQ  +K R+GR
Sbjct:    1 MKKAVQRRPDLKLIVTSATLDAVKFSTFFFEAPIFTIPGRTFPVEILYTKEAESDYLDAALITVMQIHL--TEPPGDILVFLTGQEEIDTACEIL---YERTKSLGPDVPELIILPVYSALPSEMQTRIFDPAPPGSRKCVIATNIAETSLTIDGIYYVVDPGFVKQNVYNSKSGMDALVVTPISQAQAKQRSGR 190          
BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. Ensembl Nematostella
Match: EDO32531 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SUS3])

HSP 1 Score: 249.98 bits (637), Expect = 5.543e-71
Identity = 134/329 (40.73%), Postives = 204/329 (62.01%), Query Frame = 1
Query: 1924 ISLKPLSPSTGYGMDSLQIYPISQASANQRAGRAGRTGPGICYRLYTINQYENDMLPMTVPEIQRTNLTNVVLLLKSLNVQDLMKFYFMDSPPQDNILNSMYHLWMLGALDAVGN---LTELGRTMSEFPLDPSLSKMLITSVKHGCSEEMLTIVSMLSVPAIFSRPTGHQEESDCAHEKFQVAESDHLTLLNVYRLWRESGYSANFCKKYYIHNKSMKRVREVRQQLKEIMIKLKMSLASSG-DFDMIRYCICSAYFNQAARWKGLGEYVNLRSGVVCHLHPTSSLYNMAFNADYVVYHELLMTSKEFMNYVTFVDARWLAELGHMFY 2898
            +  K   P TG  +D L++ P+S+A A QR GRAGR   G+CYRLYT  Q+E  +   TVPEIQR NL++V+L L +L + D+  F FMD P  D I  ++  L +L AL  +     LT LG+ M++FPL+P L+K+++ S   GCSEE+LT+V++LSV +I   P   ++ +    +KF  +E D +TLLN+YR +++ G + ++C +++I+ + MK V ++R+QL+EI I+L++ L S G D   +R C+C   F  +A  +  G Y  L       +HP+SSL+ M+  A YVVY+EL+ TSK +M  V+ V   WL E    F+
Sbjct:  359 VKAKGFHPKTG--LDMLRVQPVSKAQARQRLGRAGRECSGVCYRLYTEEQFEQ-LAEATVPEIQRCNLSSVILQLMALGIADIASFDFMDKPSADAIDGALKQLVILEALQEINGDYKLTSLGQKMAQFPLEPRLAKVILASETLGCSEEILTVVALLSVDSITYTPQSKRDHALKVRKKFLSSEGDQITLLNIYRAYKQCGMNKSWCAEHFINTRVMKLVMDIRKQLREICIRLEIKLQSCGKDSATLRQCLCRGLFMNSAELQLDGTYQTLNHRETVAIHPSSSLF-MSKPA-YVVYNELVHTSKCYMRDVSVVSCDWLLEAAPGFF 682          

HSP 2 Score: 236.498 bits (602), Expect = 3.024e-66
Identity = 146/314 (46.50%), Postives = 205/314 (65.29%), Query Frame = 3
Query: 1050 SEQKSKIDDQRKRLPIFKVRRDLIRLIRENQVIVIVGETGSGKTTQLTQYLLEENYHHKGIIGCTQPXXXXXXXXXXXXXDEVGTKLGDKVGYSIRFEDCKSENTMIKYMTDGIXXXXXXXXXXXXAYSVIIMDEAHERTLNTDILFGLLREIIQRRSD-----LHLVVTSATMDAEKFSNFFGKCEIFKIPGRTFPVDVIYEKSSILDPVAAAVNKAVDTH----LGADSKDGDILVFMSGQEDVEVTCDKI-DETLEKVKDTNPPDLKVEPLYSQLSSERQSEIFKINTSGIRRCIVATNIAETSVTLDGIR 1961
            +   S I  Q+  LPIF  R+ LI  IR  Q ++IVGETGSGKTTQ+ QYL E       +I CTQPRRVAA+S+A+RV+ E+G +LG++VGY++RFED  S  T IKYMTDG+LLRES+ D  L  YSVII+DEAHERT++TD+LFG+++     R D     L +VV SAT++A +FS +FG  E+  I GR  PV+++Y     +D + AA+   +  H    LG     GDILVF++GQ+++E     + D +L    D   P L V P+++ L S +Q ++F+    G R+ I++TNIAETSVT+ G++
Sbjct:   45 THASSPIQRQKLSLPIFSARKSLITEIRNRQNVIIVGETGSGKTTQIPQYLYEAKVARNSVIACTQPRRVAAISIAQRVSREMGVQLGEEVGYTVRFEDVTSTKTRIKYMTDGMLLRESIGDSLLKRYSVIILDEAHERTIHTDVLFGIVKGAQISRKDKGMLPLKIVVMSATLEAHQFSEYFGSAEVLYIEGRQHPVELMYAVEPQVDYMHAALITIMQLHQEKPLG-----GDILVFLTGQDEIESLSKLVSDCSLHCPPDC--PQLLVCPMFAALPSSQQMQVFRPAIPGARKVILSTNIAETSVTIPGVK 351          
BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. Ensembl Nematostella
Match: EDO32629 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SUG0])

HSP 1 Score: 216.083 bits (549), Expect = 3.300e-61
Identity = 114/249 (45.78%), Postives = 157/249 (63.05%), Query Frame = 1
Query: 1963 MDSLQIYPISQASANQRAGRAGRTGPGICYRLYTINQYENDMLPMTVPEIQRTNLTNVVLLLKSLNVQDLMKFYFMDSPPQDNILNSMYHLWMLGALDAVGNLTELGRTMSEFPLDPSLSKMLITSVKHGCSEEMLTIVSMLSVPAIFSRPTGHQEESDCAHEKFQVAESDHLTLLNVYRLWRESGYSANFCKKYYIHNKSMKRVREVRQQLKEIMIKLKMSLASSGDFDM------IRYCICSAYFNQ 2691
            ++SL +  IS+ASA QRAGRAGRT PG C+RLYT   ++ +M P T PEI R+NL  VVL LK L + DL+ F FMD P  + ++ ++  L  LGALD  G+LTELG  M+EFPLDP L+KM+I S +  CS E+L+I SMLSVP +F RP   ++ +D +  KF   + DHLTLLNVY  ++++     +C   +I ++SMK    VR QL  IM +  +   S+ DF+       IR  + S +F Q
Sbjct:  220 VESLLVSAISRASAQQRAGRAGRTRPGKCFRLYTEKAFQEEMPPNTYPEILRSNLGTVVLQLKKLGIDDLVHFDFMDPPAPETLMRALELLNYLGALDDNGDLTELGSMMAEFPLDPQLAKMVIASCEFNCSNEILSITSMLSVPQVFLRPNEAKKAADESKMKFAHIDGDHLTLLNVYHAYKQNHEDTQWCYDNFIQHRSMKSADNVRGQLARIMDRFNLQRRST-DFNSRDYYLNIRKALVSGFFMQ 467          

HSP 2 Score: 166.777 bits (421), Expect = 3.302e-44
Identity = 102/245 (41.63%), Postives = 150/245 (61.22%), Query Frame = 3
Query: 1371 KYMTDGIXXXXXXXXXXXXAYSVIIMDEAHERTLNTDILFGLLREIIQRRSDLHLVVTSATMDAEKFSNFFGKCEIFKIPGRTFPVDVIYEKSSILDPVAAAVNKAVDTHLGADSKDGDILVFMSGQEDVEVTCDKIDETLEKVKDTNPPDLKVEPLYSQLSSERQSEIF------KINTSGIRRCIVATNIAETSVTLDG--------------------IRNGLVADISHQSSQC*SKSRAGR 2027
            +YMTDG+LLRE++ D  LD Y+VI++DEAHERTL TDIL GLL+E+ ++R DL +++ SAT+DA KF ++F  C +  IPGRT PV++ Y      D + AA+   +  H+  + K GDIL+F++GQE++E  C +I + ++ +  +   +LK  PLYS L   +Q  IF      + N +  R+C+VATNIAETS+T+DG                    + + LV+ IS  S+Q     RAGR
Sbjct:    1 RYMTDGMLLREAMTDPLLDRYAVILLDEAHERTLATDILMGLLKEVAKQRDDLKIIIMSATLDAGKFQDYFDHCPLMTIPGRTHPVEIFYTPEPERDYLEAAIRTVIQIHMVEEVK-GDILLFLTGQEEIEEACKRIKKEVDNL-GSEIGELKCIPLYSTLPPTQQQRIFEAAPPDRPNGAIGRKCVVATNIAETSLTIDGVVFVIDPGFSKQKVYNPRIRVESLLVSAISRASAQ----QRAGR 239          
BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. Ensembl Medaka
Match: dhx38 (DEAH-box helicase 38 [Source:NCBI gene;Acc:101155586])

HSP 1 Score: 500.745 bits (1288), Expect = 0.000e+0
Identity = 296/622 (47.59%), Postives = 411/622 (66.08%), Query Frame = 3
Query:  234 NIDRHKQWEEMTPSTSLRDE--------TGRYSEIRTPMIDRSIKTPSVTSWDEDDVTFKRPDTVTSFRRNDKSEIKWSP---------------------------DKNEEELDREWYLND-GME--YNPFPNFSDEVLEKKKQQLQRQTSIVAKSRKSARAKQFEETNRAWEESKLINSGAVVRKGFNVEIDNEDTGEDRIHLHVHNILPPFLDGRIVFTKQFEPVIPVKDENSDMAKIAKNGSQMVKNYRDIKEKMKLQKRTLDMTGTNFGKLMGLEKKE-------TLDKWDETNYKDSMKYCDKITDK--IALDSEQKSKIDDQRKRLPIFKVRRDLIRLIRENQVIVIVGETGSGKTTQLTQYLLEENYHHKGIIGCTQPXXXXXXXXXXXXXDEVGTKLGDKVGYSIRFEDCKSENTMIKYMTDGIXXXXXXXXXXXXAYSVIIMDEAHERTLNTDILFGLLREIIQRRSDLHLVVTSATMDAEKFSNFFGKCEIFKIPGRTFPVDVIYEKSSILDPVAAAVNKAVDTHLGADSKDGDILVFMSGQEDVEVTCDKIDETLEKVKDTNPPDLKVEPLYSQLSSERQSEIFKINTSGIRRCIVATNIAETSVTLDGI 1958
            N  RH QWE  +P  S RD         +GR SE +    DRS++         DD     P    +   ND+  +  +P                           ++++++ DR+WY+ D G +  +NPF + S+E ++K++Q LQ+QT    + R SA+ +Q  E N  WE ++++ SG V R    V+ D E+    ++HL VHN++PPFLDGRIVFTKQ EPVIPVKD  SDMA I++ GSQ+V+ +R+ KE+ K Q +  ++ GT  G +MG++K E        + +  + +YK   K+ D + +K   + D  +K  + +QR+ LPIF VR+ L+ +IR+N ++++VGETGSGKTTQLTQYL E+ Y   G++GCTQPRRVAA+SVAKRV++E+G+ LG++VGY+IRFEDC SE+TMIKYMTDGILLRESLR+ DLD YS +IMDEAHER+LNTD+LFGLLRE++ RR+DL L+VTSATMD++KF+ FFG   IF IPGRTFPVD+++ K+   D V AAV +A+  HL      GDIL+FM GQED+EVT D+I E L ++   N P L V P+YSQL S+ Q++IF+    G+R+CIVATNIAETS+T+DGI
Sbjct:  244 NSVRHSQWESPSPFPSPRDSDRSERSHRSGRESERK----DRSVRGRYT-----DDTPLPTPSYKYNEWANDRKHLGSTPRLSQGKGRKEGAEGGIMFDKESEKEEWEEDQKQADRDWYMMDEGYDEFHNPFTSTSEEYVKKREQILQKQT----QKRISAQKRQINEDNERWETNRMLTSGVVQR--LEVDEDFEEDNATKVHLLVHNLVPPFLDGRIVFTKQPEPVIPVKDATSDMAIISRKGSQLVRKHREQKERKKAQHKHWELAGTKLGDIMGIKKTEEEEPGGKPVGEDGKVDYKADQKFADHMKEKSEASSDFAKKKSLLEQRQYLPIFAVRQQLLNIIRDNSIVIVVGETGSGKTTQLTQYLHEDGYTRYGMVGCTQPRRVAAMSVAKRVSEEIGSNLGEEVGYAIRFEDCTSESTMIKYMTDGILLRESLRESDLDHYSAVIMDEAHERSLNTDVLFGLLREVVSRRNDLKLIVTSATMDSDKFAAFFGNVPIFHIPGRTFPVDILFSKTPQEDYVEAAVKQALQIHLSG--MVGDILIFMPGQEDIEVTSDQIVERLGELD--NAPPLAVLPIYSQLPSDLQAKIFQKAPDGVRKCIVATNIAETSLTVDGI 846          

HSP 2 Score: 477.633 bits (1228), Expect = 0.000e+0
Identity = 224/364 (61.54%), Postives = 281/364 (77.20%), Query Frame = 1
Query: 1960 GMDSLQIYPISQASANQRAGRAGRTGPGICYRLYTINQYENDMLPMTVPEIQRTNLTNVVLLLKSLNVQDLMKFYFMDSPPQDNILNSMYHLWMLGALDAVGNLTELGRTMSEFPLDPSLSKMLITSVKHGCSEEMLTIVSMLSVPAIFSRPTGHQEESDCAHEKFQVAESDHLTLLNVYRLWRESGYSANFCKKYYIHNKSMKRVREVRQQLKEIMIKLKMSLASSG-DFDMIRYCICSAYFNQAARWKGLGEYVNLRSGVVCHLHPTSSLYNMAFNADYVVYHELLMTSKEFMNYVTFVDARWLAELGHMFYSVKEKGQSVTDRMLKEQQTVERMETEMRINEKANLKKLLDNRVIESERRA 3048
            GMD+LQ+YPISQA+ANQR+GRAGRTGPG CYRLYT + Y+N+ML  T+PEIQRTNL NVVLLLKSL VQDL+ F+FMD PP+DN+LNSMY LW+LGALD  G LT  GR M EFPLDP+LSKMLI S   GCS ++L IVSMLSVPAIF RP G +EESD   EKF V ESDHLT LNVY  W+ + YS+ +C  ++IH K+M++VREVR QLK+IM++ KM+L S G D+D+IR CIC+AYF+QAA+ KG+GEYVN+R+G+ CHLHPTSSL+ M +  DY++YHEL+MT+KE+M  VT VD  WLAELG MFYSVK+ G+S  +   + +  +  ME EM     A  ++ L  R  E E+++
Sbjct:  865 GMDALQVYPISQANANQRSGRAGRTGPGQCYRLYTQSAYKNEMLTTTIPEIQRTNLANVVLLLKSLGVQDLLLFHFMDPPPEDNMLNSMYQLWILGALDNTGALTPTGRLMVEFPLDPALSKMLIVSCDMGCSADILIIVSMLSVPAIFYRPKGREEESDQVREKFSVPESDHLTYLNVYNQWKNNNYSSIWCNDHFIHTKAMRKVREVRSQLKDIMVQQKMNLVSCGSDWDIIRKCICAAYFHQAAKLKGIGEYVNVRTGMPCHLHPTSSLFGMGYTPDYIIYHELVMTTKEYMQCVTAVDGEWLAELGPMFYSVKQAGRSRQENRRRAKDEITNMEEEM-----AMAQEQLRTRREEQEKKS 1223          
BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. Ensembl Medaka
Match: dhx15 (pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15 [Source:NCBI gene;Acc:101166992])

HSP 1 Score: 247.284 bits (630), Expect = 4.702e-131
Identity = 145/309 (46.93%), Postives = 212/309 (68.61%), Query Frame = 3
Query: 1065 KIDDQRKRLPIFKVRRDLIRLIRENQVIVIVGETGSGKTTQLTQYLLEENYHHKG---IIGCTQPXXXXXXXXXXXXXDEVGTKLGDKVGYSIRFEDCKSENTMIKYMTDGIXXXXXXXXXXXXAYSVIIMDEAHERTLNTDILFGLLREIIQRRSDLHLVVTSATMDAEKFSNFFGKCEIFKIPGRTFPVDVIYEKSSILDPVAAAVNKAVDTHLGADSKDGDILVFMSGQEDVEVTCDKIDETLEKVKDTNPP--DLKVEPLYSQLSSERQSEIF------KINTSGIRRCIVATNIAETSVTLDGI 1958
            +I  +R +LP+++ R     ++  NQ  V+VGETGSGKTTQ+ Q+ ++     +G    + CTQPRRVAA+SVA+RVADE+   LG +VGYSIRFEDC S  T++KYMTDG+LLRE++ D  L+ Y VII+DEAHERTL TDIL G+L+EI+++R+DL ++V SAT+DA KF  +F  C +  IPGRT PV++ Y      D + AA+   +  H+  + ++GD+L+F++GQE+++  C +I     +V+D  P   D+K+ PLYS L  ++Q  IF      K N +  R+ +V+TNIAETS+T+DG+
Sbjct:   68 EILKKRLQLPVWEYRERFTEILMRNQSFVLVGETGSGKTTQIPQWCVDMVRSLQGPKRAVACTQPRRVAAMSVAQRVADEMDVMLGQEVGYSIRFEDCSSAKTVLKYMTDGMLLREAMNDPLLERYGVIILDEAHERTLATDILMGVLKEIVRQRTDLKVIVMSATLDAGKFQVYFDNCPLLTIPGRTHPVEIFYTPEPERDYLEAAIRTVIQLHM-CEEEEGDVLLFLTGQEEIDEACKRIK---REVEDLGPEVGDMKIIPLYSTLPPQQQQRIFEPSPPNKPNGAIGRKVVVSTNIAETSLTIDGV 372          

HSP 2 Score: 242.662 bits (618), Expect = 4.702e-131
Identity = 130/332 (39.16%), Postives = 199/332 (59.94%), Query Frame = 1
Query: 1963 MDSLQIYPISQASANQRAGRAGRTGPGICYRLYTINQYENDMLPMTVPEIQRTNLTNVVLLLKSLNVQDLMKFYFMDSPPQDNILNSMYHLWMLGALDAVGNLTELGRTMSEFPLDPSLSKMLITSVKHGCSEEMLTIVSMLSVPAIFSRPTGHQEESDCAHEKFQVAESDHLTLLNVYRLWRESGYSANFCKKYYIHNKSMKRVREVRQQLKEIMIKLKM----SLASSGDFDM-IRYCICSAYFNQAARWKGLGEYVNLRSGVVCHLHPTSSLYNMAFNADYVVYHELLMTSKEFMNYVTFVDARWLAELGHMFYSVKEKGQSVTDRMLK 2943
            ++SL +  +S+ASA QRAGRAGRT PG C+RLYT   Y+ +M   T PEI R+NL  VVL LK L + DL+ F FMD P  + ++ ++  L  L AL+  G+LTELG  M+EFPLDP L+KM+I S ++ CS E+LTI +MLSVP  F RPT  ++ +D +  +F   + DHLTLLNVY  ++++  S  +C   +++ +S+     VRQQL  IM +  +    +  SS D+ + IR  + + +F Q A  +  G Y+ ++   V  LHP++ L +     ++V+Y+E ++T+K ++   T V   WL  +   +Y +    Q    R L+
Sbjct:  392 VESLLVTAVSKASAQQRAGRAGRTRPGKCFRLYTEKAYKTEMQDNTYPEILRSNLGTVVLQLKKLGIDDLVHFDFMDPPAPETLMRALELLNFLAALNDDGDLTELGAMMAEFPLDPQLAKMVIASCEYNCSNEILTITAMLSVPQCFVRPTEAKKVADESKLRFAHIDGDHLTLLNVYHAFKQNHESTQWCYDNFVNYRSLMSADNVRQQLSRIMERFNLPRRSTEFSSRDYYINIRRALVTGFFMQVAHLERTGHYLTVKDNQVVQLHPSTVLDH---KPEWVLYNEFVLTTKNYIRTCTDVKPEWLMRIAPQYYDLGNFPQCEARRQLE 720          
BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. Ensembl Medaka
Match: dhx15 (pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15 [Source:NCBI gene;Acc:101166992])

HSP 1 Score: 247.284 bits (630), Expect = 5.153e-131
Identity = 145/309 (46.93%), Postives = 212/309 (68.61%), Query Frame = 3
Query: 1065 KIDDQRKRLPIFKVRRDLIRLIRENQVIVIVGETGSGKTTQLTQYLLEENYHHKG---IIGCTQPXXXXXXXXXXXXXDEVGTKLGDKVGYSIRFEDCKSENTMIKYMTDGIXXXXXXXXXXXXAYSVIIMDEAHERTLNTDILFGLLREIIQRRSDLHLVVTSATMDAEKFSNFFGKCEIFKIPGRTFPVDVIYEKSSILDPVAAAVNKAVDTHLGADSKDGDILVFMSGQEDVEVTCDKIDETLEKVKDTNPP--DLKVEPLYSQLSSERQSEIF------KINTSGIRRCIVATNIAETSVTLDGI 1958
            +I  +R +LP+++ R     ++  NQ  V+VGETGSGKTTQ+ Q+ ++     +G    + CTQPRRVAA+SVA+RVADE+   LG +VGYSIRFEDC S  T++KYMTDG+LLRE++ D  L+ Y VII+DEAHERTL TDIL G+L+EI+++R+DL ++V SAT+DA KF  +F  C +  IPGRT PV++ Y      D + AA+   +  H+  + ++GD+L+F++GQE+++  C +I     +V+D  P   D+K+ PLYS L  ++Q  IF      K N +  R+ +V+TNIAETS+T+DG+
Sbjct:   41 EILKKRLQLPVWEYRERFTEILMRNQSFVLVGETGSGKTTQIPQWCVDMVRSLQGPKRAVACTQPRRVAAMSVAQRVADEMDVMLGQEVGYSIRFEDCSSAKTVLKYMTDGMLLREAMNDPLLERYGVIILDEAHERTLATDILMGVLKEIVRQRTDLKVIVMSATLDAGKFQVYFDNCPLLTIPGRTHPVEIFYTPEPERDYLEAAIRTVIQLHM-CEEEEGDVLLFLTGQEEIDEACKRIK---REVEDLGPEVGDMKIIPLYSTLPPQQQQRIFEPSPPNKPNGAIGRKVVVSTNIAETSLTIDGV 345          

HSP 2 Score: 242.662 bits (618), Expect = 5.153e-131
Identity = 130/332 (39.16%), Postives = 199/332 (59.94%), Query Frame = 1
Query: 1963 MDSLQIYPISQASANQRAGRAGRTGPGICYRLYTINQYENDMLPMTVPEIQRTNLTNVVLLLKSLNVQDLMKFYFMDSPPQDNILNSMYHLWMLGALDAVGNLTELGRTMSEFPLDPSLSKMLITSVKHGCSEEMLTIVSMLSVPAIFSRPTGHQEESDCAHEKFQVAESDHLTLLNVYRLWRESGYSANFCKKYYIHNKSMKRVREVRQQLKEIMIKLKM----SLASSGDFDM-IRYCICSAYFNQAARWKGLGEYVNLRSGVVCHLHPTSSLYNMAFNADYVVYHELLMTSKEFMNYVTFVDARWLAELGHMFYSVKEKGQSVTDRMLK 2943
            ++SL +  +S+ASA QRAGRAGRT PG C+RLYT   Y+ +M   T PEI R+NL  VVL LK L + DL+ F FMD P  + ++ ++  L  L AL+  G+LTELG  M+EFPLDP L+KM+I S ++ CS E+LTI +MLSVP  F RPT  ++ +D +  +F   + DHLTLLNVY  ++++  S  +C   +++ +S+     VRQQL  IM +  +    +  SS D+ + IR  + + +F Q A  +  G Y+ ++   V  LHP++ L +     ++V+Y+E ++T+K ++   T V   WL  +   +Y +    Q    R L+
Sbjct:  365 VESLLVTAVSKASAQQRAGRAGRTRPGKCFRLYTEKAYKTEMQDNTYPEILRSNLGTVVLQLKKLGIDDLVHFDFMDPPAPETLMRALELLNFLAALNDDGDLTELGAMMAEFPLDPQLAKMVIASCEYNCSNEILTITAMLSVPQCFVRPTEAKKVADESKLRFAHIDGDHLTLLNVYHAFKQNHESTQWCYDNFVNYRSLMSADNVRQQLSRIMERFNLPRRSTEFSSRDYYINIRRALVTGFFMQVAHLERTGHYLTVKDNQVVQLHPSTVLDH---KPEWVLYNEFVLTTKNYIRTCTDVKPEWLMRIAPQYYDLGNFPQCEARRQLE 693          
BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. Ensembl Medaka
Match: DHX15 (DEAH-box helicase 15 [Source:NCBI gene;Acc:105357480])

HSP 1 Score: 244.973 bits (624), Expect = 3.296e-130
Identity = 130/343 (37.90%), Postives = 207/343 (60.35%), Query Frame = 1
Query: 1963 MDSLQIYPISQASANQRAGRAGRTGPGICYRLYTINQYENDMLPMTVPEIQRTNLTNVVLLLKSLNVQDLMKFYFMDSPPQDNILNSMYHLWMLGALDAVGNLTELGRTMSEFPLDPSLSKMLITSVKHGCSEEMLTIVSMLSVPAIFSRPTGHQEESDCAHEKFQVAESDHLTLLNVYRLWRESGYSANFCKKYYIHNKSMKRVREVRQQLKEIMIKLKMSLAS----SGDFDM-IRYCICSAYFNQAARWKGLGEYVNLRSGVVCHLHPTSSLYNMAFNADYVVYHELLMTSKEFMNYVTFVDARWLAELGHMFYSVKEKGQSVTDRMLKEQQTVERMETE 2976
            ++SL +  IS+ASA QRAGRAGRT PG C+RLYT   Y+ +M   T PEI R+NL +VVL LK L + DL+ F FMD P  + ++ ++  L  L AL+  G+LTELG  M+EFPLDP L+KM+I S +  CS E+L+I +MLSVP  F RPT  ++ +D +  +F   + DHLT+LNVY  ++++  S  +C   +++ +S+     VRQQL  IM +  +   S    S D+ + IR  +C+ +F Q A  +  G Y+ ++   V  LHP++ L +     ++V+Y+E ++T+K ++   T +   WL ++   +Y +    Q    R L  ++ + ++E++
Sbjct:  414 VESLLVTAISKASAQQRAGRAGRTRPGKCFRLYTEKAYKTEMQDNTYPEILRSNLGSVVLQLKKLGIDDLVHFDFMDPPAPETLMRALELLNYLAALNDDGDLTELGSMMAEFPLDPQLAKMVIASCEFNCSNEILSITAMLSVPQCFVRPTEAKKAADESKMRFAHIDGDHLTMLNVYHAFKQNHESNQWCYDNFVNYRSLMSADNVRQQLSRIMDRFNLPRRSTEFTSRDYYINIRRALCTGFFMQVAHLERTGHYLTVKDNQVVQLHPSTVLDH---KPEWVLYNEFVLTTKNYIRTCTDIKPEWLVKIAPQYYDMSNFPQCEAKRQL--ERIIAKLESK 751          

HSP 2 Score: 242.276 bits (617), Expect = 3.296e-130
Identity = 144/309 (46.60%), Postives = 208/309 (67.31%), Query Frame = 3
Query: 1065 KIDDQRKRLPIFKVRRDLIRLIRENQVIVIVGETGSGKTTQLTQYLLEENYHHKG---IIGCTQPXXXXXXXXXXXXXDEVGTKLGDKVGYSIRFEDCKSENTMIKYMTDGIXXXXXXXXXXXXAYSVIIMDEAHERTLNTDILFGLLREIIQRRSDLHLVVTSATMDAEKFSNFFGKCEIFKIPGRTFPVDVIYEKSSILDPVAAAVNKAVDTHLGADSKDGDILVFMSGQEDVEVTCDKIDETLEKVKDTNPP--DLKVEPLYSQLSSERQSEIF------KINTSGIRRCIVATNIAETSVTLDGI 1958
            +I  +R +LP+++ +     +I   Q  V+VGETGSGKTTQ+ Q+ ++      G    + CTQPRRVAA+SVA+RVADE+   LG +VGYSIRFEDC S  T++KYMTDG+LLRE++ D  L+ Y VII+DEAHERTL TDIL G+L+E++++RSDL ++V SAT+DA KF  +F  C +  IPGRT PV++ Y      D + AA+   +  H+  + ++GD L+F++GQE+++  C +I     +V D  P   D+K+ PLYS L  ++Q  IF      K N +  R+ +V+TNIAETS+T+DG+
Sbjct:   90 EILKKRLQLPVWEYKESFTNIITRQQSFVLVGETGSGKTTQIPQWCVDMVRGLPGPKRAVACTQPRRVAAMSVAQRVADEMDVMLGQEVGYSIRFEDCSSAKTILKYMTDGMLLREAMNDPLLERYGVIILDEAHERTLATDILMGVLKEVVRQRSDLKVIVMSATLDAGKFQVYFDNCPLLTIPGRTHPVEIFYTPEPERDYLEAAIRTVIQIHM-CEEEEGDCLLFLTGQEEIDEACKRIK---REVDDLGPEVGDIKIIPLYSTLPPQQQQRIFEPPPPRKPNGAIGRKVVVSTNIAETSLTIDGV 394          
BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. Ensembl Medaka
Match: dhx33 (DEAH-box helicase 33 [Source:NCBI gene;Acc:101170865])

HSP 1 Score: 233.802 bits (595), Expect = 3.096e-118
Identity = 121/320 (37.81%), Postives = 196/320 (61.25%), Query Frame = 1
Query: 1960 GMDSLQIYPISQASANQRAGRAGRTGPGICYRLYTINQYENDMLPMTVPEIQRTNLTNVVLLLKSLNVQDLMKFYFMDSPPQDNILNSMYHLWMLGALDAVGN---LTELGRTMSEFPLDPSLSKMLITSVKHGCSEEMLTIVSMLSVPAIFSRPTGHQEESDCAHEKFQVAESDHLTLLNVYRLWRESGYSANFCKKYYIHNKSMKRVREVRQQLKEIMIKLKMSLASSG-DFDMIRYCICSAYFNQAARWKGLGEYVNLRSGVVCHLHPTSSLYNMAFNADYVVYHELLMTSKEFMNYVTFVDARWLAELGHMFYSVK 2907
            G++ L +  +S+A A QRAGRAGR   G CYRLYT  +++N  LPMTVPEIQR NL  V+L L +L + D+M F FM  P  + + +++ HL +LGA++       LT LG+ M+ FPL+P  +K ++ S ++ CSEE+L+IV +LSV  +   P   +++   A +KF  +E DH+TLLN+YR +++   +  +C++ ++++++M  V++V+ QL++I +KL + L S G D   +R C+    F  AA  +  G Y+ L +     +HP+S L+       YV+++ELL TS+ +M  +  VD  WL E    ++  K
Sbjct:  359 GLEVLAVQRVSKAQAWQRAGRAGREESGFCYRLYTEQEFDN-FLPMTVPEIQRCNLAGVMLQLMALGIPDVMNFDFMSKPSPEAVRSAVDHLELLGAVEKREGQVLLTPLGKKMASFPLEPRYAKTILLSPEYSCSEEILSIVCLLSVDTVLYNPPARRDDVLAARKKFMTSEGDHMTLLNIYRAFKKVSGNKEWCRENFVNSRNMGLVKDVQAQLRDICLKLNLKLESCGSDTASVRRCLAHGMFLNAAELQLDGSYLALDTHQPVTIHPSSVLFQA--KPAYVIFNELLHTSRCYMRDLCLVDPDWLLEAAPEYFGRK 675          

HSP 2 Score: 213.772 bits (543), Expect = 3.096e-118
Identity = 131/305 (42.95%), Postives = 195/305 (63.93%), Query Frame = 3
Query: 1062 SKIDDQRKRLPIFKVRRDLIRLIRENQVIVIVGETGSGKTTQLTQYLLEENYHHKGIIGCTQPXXXXXXXXXXXXXDEVGTKLGDKVGYSIRFEDCKSENTMIKYMTDGIXXXXXXXXXXXXAYSVIIMDEAHERTLNTDILFGLLREIIQRRSDLH-----LVVTSATMDAEKFSNFFGKCEIFKIPGRTFPVDVIYEKSSILDPVAAAVNKAVDTHLGADSKDGDILVFMSGQEDVEVTCDKIDETLEKVKDTNPPDLKVEPLYSQLSSERQSEIFKINTSGIRRCIVATNIAETSVTLDGIR 1961
            + I+ QRK+LPI++ +  L+  +R+ Q  +I+GETGSGKTTQ+ QYL E      G++  TQPRRVAA+S+A RVA+E  T++G  VGY++RFED  S  T +K+MTDG+LLRE++ D  L  Y+V+++DEAHERT++TD+LFG+++   +RR +L+     ++V SATMD + FS +F K  +  + GR  P+ + Y K    D + AA+      H  A     DILVFM+GQE++E       +  + V D     + V PLY+ L   +Q  +F+      R+ I++TNIAETSVT+ GI+
Sbjct:   39 TPIEVQRKQLPIYQAKPHLLNQLRQLQNAIIIGETGSGKTTQIPQYLYEAGIGRLGMVAITQPRRVAAISLAGRVAEEKRTQIGKLVGYTVRFEDVTSSETKLKFMTDGMLLREAIGDPLLLKYTVVVLDEAHERTVHTDVLFGVVKTAQRRRRELNKIPLKVIVMSATMDVDLFSEYFNKSPVLYLEGRQHPIQIFYTKHQQSDYLQAALVSIFQIHQEAPPAH-DILVFMTGQEEIEALARTCRDISKHVPDGC-GSMVVLPLYASLPPVQQLRVFQPAPKSCRKVILSTNIAETSVTISGIK 341          
BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. Planmine SMEST
Match: SMESG000023160.1 (SMESG000023160.1)

HSP 1 Score: 1253.04 bits (3241), Expect = 0.000e+0
Identity = 637/637 (100.00%), Postives = 637/637 (100.00%), Query Frame = 3
Query:   51 MNSECRKLSKRNYRSRDDPTPINVKLDNEEYIKRQNERKQRDEKRKIGESFGSKSKIKKIENIDRHKQWEEMTPSTSLRDETGRYSEIRTPMIDRSIKTPSVTSWDEDDVTFKRPDTVTSFRRNDKSEIKWSPDKNEEELDREWYLNDGMEYNPFPNFSDEVLEKKKQQLQRQTSIVAKSRKSARAKQFEETNRAWEESKLINSGAVVRKGFNVEIDNEDTGEDRIHLHVHNILPPFLDGRIVFTKQFEPVIPVKDENSDMAKIAKNGSQMVKNYRDIKEKMKLQKRTLDMTGTNFGKLMGLEKKETLDKWDETNYKDSMKYCDKITDKIALDSEQKSKIDDQRKRLPIFKVRRDLIRLIRENQVIVIVGETGSGKTTQLTQYLLEENYHHKGIIGCTQPXXXXXXXXXXXXXDEVGTKLGDKVGYSIRFEDCKSENTMIKYMTDGIXXXXXXXXXXXXAYSVIIMDEAHERTLNTDILFGLLREIIQRRSDLHLVVTSATMDAEKFSNFFGKCEIFKIPGRTFPVDVIYEKSSILDPVAAAVNKAVDTHLGADSKDGDILVFMSGQEDVEVTCDKIDETLEKVKDTNPPDLKVEPLYSQLSSERQSEIFKINTSGIRRCIVATNIAETSVTLDGIR 1961
            MNSECRKLSKRNYRSRDDPTPINVKLDNEEYIKRQNERKQRDEKRKIGESFGSKSKIKKIENIDRHKQWEEMTPSTSLRDETGRYSEIRTPMIDRSIKTPSVTSWDEDDVTFKRPDTVTSFRRNDKSEIKWSPDKNEEELDREWYLNDGMEYNPFPNFSDEVLEKKKQQLQRQTSIVAKSRKSARAKQFEETNRAWEESKLINSGAVVRKGFNVEIDNEDTGEDRIHLHVHNILPPFLDGRIVFTKQFEPVIPVKDENSDMAKIAKNGSQMVKNYRDIKEKMKLQKRTLDMTGTNFGKLMGLEKKETLDKWDETNYKDSMKYCDKITDKIALDSEQKSKIDDQRKRLPIFKVRRDLIRLIRENQVIVIVGETGSGKTTQLTQYLLEENYHHKGIIGCTQPRRVAAVSVAKRVADEVGTKLGDKVGYSIRFEDCKSENTMIKYMTDGILLRESLRDEDLDAYSVIIMDEAHERTLNTDILFGLLREIIQRRSDLHLVVTSATMDAEKFSNFFGKCEIFKIPGRTFPVDVIYEKSSILDPVAAAVNKAVDTHLGADSKDGDILVFMSGQEDVEVTCDKIDETLEKVKDTNPPDLKVEPLYSQLSSERQSEIFKINTSGIRRCIVATNIAETSVTLDGIR
Sbjct:    1 MNSECRKLSKRNYRSRDDPTPINVKLDNEEYIKRQNERKQRDEKRKIGESFGSKSKIKKIENIDRHKQWEEMTPSTSLRDETGRYSEIRTPMIDRSIKTPSVTSWDEDDVTFKRPDTVTSFRRNDKSEIKWSPDKNEEELDREWYLNDGMEYNPFPNFSDEVLEKKKQQLQRQTSIVAKSRKSARAKQFEETNRAWEESKLINSGAVVRKGFNVEIDNEDTGEDRIHLHVHNILPPFLDGRIVFTKQFEPVIPVKDENSDMAKIAKNGSQMVKNYRDIKEKMKLQKRTLDMTGTNFGKLMGLEKKETLDKWDETNYKDSMKYCDKITDKIALDSEQKSKIDDQRKRLPIFKVRRDLIRLIRENQVIVIVGETGSGKTTQLTQYLLEENYHHKGIIGCTQPRRVAAVSVAKRVADEVGTKLGDKVGYSIRFEDCKSENTMIKYMTDGILLRESLRDEDLDAYSVIIMDEAHERTLNTDILFGLLREIIQRRSDLHLVVTSATMDAEKFSNFFGKCEIFKIPGRTFPVDVIYEKSSILDPVAAAVNKAVDTHLGADSKDGDILVFMSGQEDVEVTCDKIDETLEKVKDTNPPDLKVEPLYSQLSSERQSEIFKINTSGIRRCIVATNIAETSVTLDGIR 637          

HSP 2 Score: 806.594 bits (2082), Expect = 0.000e+0
Identity = 381/381 (100.00%), Postives = 381/381 (100.00%), Query Frame = 1
Query: 1960 GMDSLQIYPISQASANQRAGRAGRTGPGICYRLYTINQYENDMLPMTVPEIQRTNLTNVVLLLKSLNVQDLMKFYFMDSPPQDNILNSMYHLWMLGALDAVGNLTELGRTMSEFPLDPSLSKMLITSVKHGCSEEMLTIVSMLSVPAIFSRPTGHQEESDCAHEKFQVAESDHLTLLNVYRLWRESGYSANFCKKYYIHNKSMKRVREVRQQLKEIMIKLKMSLASSGDFDMIRYCICSAYFNQAARWKGLGEYVNLRSGVVCHLHPTSSLYNMAFNADYVVYHELLMTSKEFMNYVTFVDARWLAELGHMFYSVKEKGQSVTDRMLKEQQTVERMETEMRINEKANLKKLLDNRVIESERRAQSVKETTYSRPRSSRYGI 3102
            GMDSLQIYPISQASANQRAGRAGRTGPGICYRLYTINQYENDMLPMTVPEIQRTNLTNVVLLLKSLNVQDLMKFYFMDSPPQDNILNSMYHLWMLGALDAVGNLTELGRTMSEFPLDPSLSKMLITSVKHGCSEEMLTIVSMLSVPAIFSRPTGHQEESDCAHEKFQVAESDHLTLLNVYRLWRESGYSANFCKKYYIHNKSMKRVREVRQQLKEIMIKLKMSLASSGDFDMIRYCICSAYFNQAARWKGLGEYVNLRSGVVCHLHPTSSLYNMAFNADYVVYHELLMTSKEFMNYVTFVDARWLAELGHMFYSVKEKGQSVTDRMLKEQQTVERMETEMRINEKANLKKLLDNRVIESERRAQSVKETTYSRPRSSRYGI
Sbjct:  655 GMDSLQIYPISQASANQRAGRAGRTGPGICYRLYTINQYENDMLPMTVPEIQRTNLTNVVLLLKSLNVQDLMKFYFMDSPPQDNILNSMYHLWMLGALDAVGNLTELGRTMSEFPLDPSLSKMLITSVKHGCSEEMLTIVSMLSVPAIFSRPTGHQEESDCAHEKFQVAESDHLTLLNVYRLWRESGYSANFCKKYYIHNKSMKRVREVRQQLKEIMIKLKMSLASSGDFDMIRYCICSAYFNQAARWKGLGEYVNLRSGVVCHLHPTSSLYNMAFNADYVVYHELLMTSKEFMNYVTFVDARWLAELGHMFYSVKEKGQSVTDRMLKEQQTVERMETEMRINEKANLKKLLDNRVIESERRAQSVKETTYSRPRSSRYGI 1035          
BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. Planmine SMEST
Match: SMESG000010022.1 (SMESG000010022.1)

HSP 1 Score: 839.336 bits (2167), Expect = 0.000e+0
Identity = 444/584 (76.03%), Postives = 507/584 (86.82%), Query Frame = 3
Query:   78 KRNYRSRDDPTPINVKLDNEEYIKRQNERKQRDEKRKIGESFGSKSKIKKIENIDRHKQWEEMTPSTSLRDETGRYSEIRTPMIDRSIKTPSVTSWDEDDVTFKRPDTVTSFRRNDKSEIKWSPDKNEEELDREWYLNDGMEYNPFPNFSDEVLEKKKQQLQRQTSIVAKSRKSARAKQFEETNRAWEESKLINSGAVVRKGFNVEIDNEDTGEDRIHLHVHNILPPFLDGRIVFTKQFEPVIPVKDENSDMAKIAKNGSQMVKNYRDIKEKMKLQKRTLDMTGTNFGKLMGLEKKETLDKWDETNYKDSMKYCDKITDKIA-LDSEQKSKIDDQRKRLPIFKVRRDLIRLIRENQVIVIVGETGSGKTTQLTQYLLEENYHHKGIIGCTQPXXXXXXXXXXXXXDEVGTKLGDKVGYSIRFEDCKSENTMIKYMTDGIXXXXXXXXXXXXAYSVIIMDEAHERTLNTDILFGLLREIIQRRSDLHLVVTSATMDAEKFSNFFGKCEIFKIPGRTFPVDVIYEKSSILDPVAAAVNKAVDTHLGADSKDGDILVFMSGQEDVEVTCDKIDETLEKVKDTNPPDL 1826
            KRNYRS D PTPIN KL+  EY KR++ERK R+ KRK G+S  S S + K+  +DRHKQWEE TPS S R+     SEIRTP I+ SIKTPS  SWD +D  F R  +V S  ++ +SE KWSP   EEELDREWYL+DGM+YNPF +F+D+ LEKKK+Q  ++    AKSRKSARA+Q+EE+N+AWEESK+I+SGA VRK FN++IDNEDTGEDRIHLHVHNILPPFLDGRI+FTKQFEPVIPVKDENSDMAKIAKNGSQMV  YRDIKEK+KLQKR LDMTGTN+GK+MGLE K+  D W E N+KDSMK+CDKIT+    +D E+K  ID+QRKRLPIFKVRRDL+R+IRENQVI+IVGETGSGKTTQLTQYLLEENYH KG+IGCTQPRRVAAVSVAKRVADEVGTKLG++VGYSIRFEDCKS+NT+IKYMTDGILLRESLRD+DLDAYSVIIMDEAHERTLNTDILFGLLREIIQRR DL L+VTSATMDA+KFS FFGKCEIFKIPGRTFPV+V+YEKSS+LDPVA AVNKAVDTHLG+D  +GDILVFMSGQEDVEVTC+KI+ETLE++KD NP +L
Sbjct:   10 KRNYRSHDYPTPINDKLEENEYKKREDERKNRELKRKYGKSEDSISNVSKLNVLDRHKQWEEPTPS-SCRNSRRESSEIRTPRINSSIKTPSACSWDVEDDVFTRSGSVVS--QSVRSEAKWSP--KEEELDREWYLDDGMDYNPFTDFTDDALEKKKKQQLQKQFNTAKSRKSARAQQYEESNKAWEESKIISSGAAVRKKFNIQIDNEDTGEDRIHLHVHNILPPFLDGRIIFTKQFEPVIPVKDENSDMAKIAKNGSQMVSAYRDIKEKLKLQKRGLDMTGTNYGKIMGLETKDKEDNWTEANFKDSMKFCDKITNNDEEMDCEEKLTIDEQRKRLPIFKVRRDLMRMIRENQVIIIVGETGSGKTTQLTQYLLEENYHRKGVIGCTQPRRVAAVSVAKRVADEVGTKLGERVGYSIRFEDCKSDNTVIKYMTDGILLRESLRDKDLDAYSVIIMDEAHERTLNTDILFGLLREIIQRRFDLRLIVTSATMDADKFSRFFGKCEIFKIPGRTFPVNVMYEKSSVLDPVAKAVNKAVDTHLGSDVNNGDILVFMSGQEDVEVTCEKINETLEELKDKNPQNL 588          
BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. Planmine SMEST
Match: SMESG000010011.1 (SMESG000010011.1)

HSP 1 Score: 673.7 bits (1737), Expect = 0.000e+0
Identity = 317/381 (83.20%), Postives = 350/381 (91.86%), Query Frame = 1
Query: 1960 GMDSLQIYPISQASANQRAGRAGRTGPGICYRLYTINQYENDMLPMTVPEIQRTNLTNVVLLLKSLNVQDLMKFYFMDSPPQDNILNSMYHLWMLGALDAVGNLTELGRTMSEFPLDPSLSKMLITSVKHGCSEEMLTIVSMLSVPAIFSRPTGHQEESDCAHEKFQVAESDHLTLLNVYRLWRESGYSANFCKKYYIHNKSMKRVREVRQQLKEIMIKLKMSLASSGDFDMIRYCICSAYFNQAARWKGLGEYVNLRSGVVCHLHPTSSLYNMAFNADYVVYHELLMTSKEFMNYVTFVDARWLAELGHMFYSVKEKGQSVTDRMLKEQQTVERMETEMRINEKANLKKLLDNRVIESERRAQSVKETTYSRPRSSRYGI 3102
            GMDSLQ+YPISQASANQRAGRAGRT PGICYRLYTI QYE+DMLPMTVPEIQRTNLTNVVLLLKSLN+ DLMKF FMDSPPQDNILNSMYHLWMLGALD VGNLT+ GR MSE+PLDPSLSKMLI S++ GCSEEMLTIVSMLSVP++FSRP G++E+SD AHEKFQVAESDHLTLLNVYRLWRESGYS+NFCKKYYIH KSMKRVREVRQQLKEIMIKLKM L S+ +FD IR CICSAYFNQAARWKGLGEYVNLRSGVVCHLHPTSSLYNMAFNADYVVYHELLMT+KE+MN+VTFVDARWLA+ GHMFYSVKE+G+S + RMLKE +TVERME +M+  E +NL+K  DNR+I+SERR  + ++ TY+RPRSSRYGI
Sbjct:    9 GMDSLQVYPISQASANQRAGRAGRTAPGICYRLYTIKQYEDDMLPMTVPEIQRTNLTNVVLLLKSLNILDLMKFPFMDSPPQDNILNSMYHLWMLGALDTVGNLTKFGRNMSEYPLDPSLSKMLIASIEIGCSEEMLTIVSMLSVPSVFSRPMGYREQSDAAHEKFQVAESDHLTLLNVYRLWRESGYSSNFCKKYYIHYKSMKRVREVRQQLKEIMIKLKMPLLSTRNFDEIRRCICSAYFNQAARWKGLGEYVNLRSGVVCHLHPTSSLYNMAFNADYVVYHELLMTTKEYMNFVTFVDARWLADSGHMFYSVKEQGESASVRMLKELKTVERMENDMKEVENSNLEK--DNRIIQSERRPHNSQDFTYTRPRSSRYGI 387          
BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. Planmine SMEST
Match: SMESG000006773.1 (SMESG000006773.1)

HSP 1 Score: 303.908 bits (777), Expect = 9.783e-156
Identity = 148/314 (47.13%), Postives = 204/314 (64.97%), Query Frame = 1
Query: 1960 GMDSLQIYPISQASANQRAGRAGRTGPGICYRLYTINQYENDMLPMTVPEIQRTNLTNVVLLLKSLNVQDLMKFYFMDSPPQDNILNSMYHLWMLGALDAVGNLTELGRTMSEFPLDPSLSKMLITSVKHGCSEEMLTIVSMLSVPAIFSRPTGHQEESDCAHEKFQVAESDHLTLLNVYRLWRESGYSANFCKKYYIHNKSMKRVREVRQQLKEIMIKLKMSLASSG-DFDMIRYCICSAYFNQAARWKGLGEYVNLRSGVVCHLHPTSSLYNMAFNADYVVYHELLMTSKEFMNYVTFVDARWLAELGHMFY 2898
            GMD L + PISQA A+QR GRAGRTGPG CYRLYT   ++ +MLP  VPEIQRTN+ + VL LK++ + DL+ F FMD+PP   ++ +M  L  L ALD  G LT+LGR M+EFPL+P L+KMLI SV  GCSEE+LTIVSMLSV   F RP    + +D    KF   E DH+TLL V+  W+ S +S  +C   +I  +S+K  ++VR+QL  IM +  + + S G +  +I+  I S +F  AAR      Y  L    V H+HP+S+L++     D+V++HEL++T+KE+M  VT +D++WL E    F+
Sbjct:  772 GMDQLIVTPISQAQADQRKGRAGRTGPGKCYRLYTERAFKEEMLPNNVPEIQRTNMASTVLALKAMGINDLLTFDFMDAPPSQTLVKAMSELHALSALDDEGLLTKLGRKMAEFPLEPMLAKMLIMSVHLGCSEEILTIVSMLSVEPAFYRPKDKADAADQKKAKFNQPEGDHITLLAVFNAWKNSKFSTPWCYDNFIQARSLKHAQDVRKQLLGIMDRYSLKIRSCGKNTGLIQKAIVSGFFRNAARKDPQEGYRTLVDQQVVHIHPSSALFHR--QPDWVIFHELVLTTKEYMREVTTIDSKWLVEFAPKFF 1083          

HSP 2 Score: 267.7 bits (683), Expect = 9.783e-156
Identity = 173/416 (41.59%), Postives = 254/416 (61.06%), Query Frame = 3
Query:  753 PPFLDGRIVFTKQFEPVIPVKDENSDMAKIAKNGSQMVKNYRDIKEKMKLQKRTLDMTGTNFGKLMGLEKKETLDKWDETNYKDSMKYCDKITD--------------KIALDSEQKSKIDDQRKRLPIFKVRRDLIRLIRENQVIVIVGETGSGKTTQLTQYLLEENYHHKGIIGCTQPXXXXXXXXXXXXXDEVGTKLGDKVGYSIRFEDCKSENTMIKYMTDGIXXXXXXXXXXXXAYSVIIMDEAHERTLNTDILFGLLREIIQRRSDLHLVVTSATMDAEKFSNFFGKCEIFKIPGRTFPVDVIYEKSSILDPVAAAVNKAVDTHLGADSKDGDILVFMSGQEDVEVTCDKIDETLEKVKDTNPPDLKVEPLYSQLSSERQSEIFKINTSGIRRCIVATNIAETSVTLDGI 1958
            P FL G+        PV  VK+ +  +   A   S + K  R+ K++ K+ ++   M+  +  K+ G +  + + K    N    +   +K +D              + A        I +QR+ LPIFK++ +L++ I +NQV+V++GETGSGKTTQ+TQY+ E        I CTQPRRVAA SVAKRVA E G  LG +VGY+IRFED  +  T IKYMTDG+LLRE L D +L+ YSVI++DEAHERT++TD+LFGLL++   +R DL L+VTSAT+++ KFS +F    IF+IPGRTF V+V+Y      D + A++N  +  HL      GD+L+F++GQE+++  C+ I   + K    + P+L + P+YS + S+ QS IF       R+ ++ATNIAETS+T++GI
Sbjct:  344 PAFLKGQFNEELTLSPVRIVKNPDGSLQNAAMMQSALQKQRRETKQQEKMNEQ---MSKVSTQKVTGKDWHDPVMKEFNNNTGSRINRNEKFSDVPEWKKYVTGGTGGRGASTQMLNKSILEQRQSLPIFKLKNELLQAINDNQVLVVIGETGSGKTTQMTQYIAEAGLIKNKKIACTQPRRVAAKSVAKRVAQEFGCLLGQEVGYTIRFEDMTTSETKIKYMTDGMLLRECLVDPELNQYSVIMLDEAHERTIHTDVLFGLLKKAATQRDDLKLIVTSATLNSVKFSEYFFGSPIFRIPGRTFEVEVLYSVEPQQDYLEASLNTVMHIHL--TEPPGDMLLFLTGQEEIDTCCEIIYNRI-KALGQDCPELLILPVYSAMPSDHQSRIFDPAPPNARKLVIATNIAETSLTINGI 753          
BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. Planmine SMEST
Match: SMESG000009126.1 (SMESG000009126.1)

HSP 1 Score: 268.855 bits (686), Expect = 4.826e-141
Identity = 136/331 (41.09%), Postives = 203/331 (61.33%), Query Frame = 1
Query: 1963 MDSLQIYPISQASANQRAGRAGRTGPGICYRLYTINQYENDMLPMTVPEIQRTNLTNVVLLLKSLNVQDLMKFYFMDSPPQDNILNSMYHLWMLGALDAVGNLTELGRTMSEFPLDPSLSKMLITSVKHGCSEEMLTIVSMLSVPAIFSRPTGHQEESDCAHEKFQVAESDHLTLLNVYRLWRESGYSANFCKKYYIHNKSMKRVREVRQQLKEIMIKLKMSL----ASSGDFDMIRYCICSAYFNQAARWKGLGEYVNLRSGVVCHLHPTSSLYNMAFNADYVVYHELLMTSKEFMNYVTFVDARWLAELGHMFYSVKEKGQSVTDRMLK 2943
            ++SL +  +S+ASA QRAGRAGRTGPG C+RLY+   Y N+++  T PEI R+NL+NV+L LK L V D++ F F+D P  DN++ +   L+ LGA+D  G LT+LGR M+EFPLDP LSK LI S    CS+++L IVSMLSVP  F RP   Q  +D A  +    E DHLTLLNVY  +  +  SA +C   +I+ +S+K   EVR QL  I+ K K+      A+S +++ I+  + + +F Q+A +   G Y+ ++   +  LHP+++L       D+V+Y+E +MT+  F+  VT ++  WL      +Y+  +       R+L+
Sbjct:  362 VESLTVTKVSRASAKQRAGRAGRTGPGKCFRLYSEASYHNELIENTYPEILRSNLSNVILSLKKLGVGDIIGFDFIDPPHPDNMMRAFDLLYYLGAIDCDGVLTDLGRMMAEFPLDPQLSKTLIASCDFNCSDDVLNIVSMLSVPPCFVRPIESQGAADAAKMRLSKTEGDHLTLLNVYNEFINNKKSAKWCSNNFINYRSLKTANEVRLQLSRILDKFKLKQISNDATSSNYENIKKSLVAGFFMQSAHFDKKGSYLTVKDTQIARLHPSTALLR---KPDWVIYNEFVMTTNNFLRIVTEINPSWLVNAAPEYYNPADFPDGRAKRILE 689          

HSP 2 Score: 253.832 bits (647), Expect = 4.826e-141
Identity = 154/312 (49.36%), Postives = 215/312 (68.91%), Query Frame = 3
Query: 1050 SEQKSKIDDQRKRLPIFKVRRDLIRLIRENQVIVIVGETGSGKTTQLTQYLLE---ENYHHKGIIGCTQPXXXXXXXXXXXXXDEVGTKLGDKVGYSIRFEDCKSENTMIKYMTDGIXXXXXXXXXXXXAYSVIIMDEAHERTLNTDILFGLLREIIQRRSDLHLVVTSATMDAEKFSNFFGKCEIFKIPGRTFPVDVIYEKSSILDPVAAAVNKAVDTHLGADSKDGDILVFMSGQEDVEVTCDKIDETLEKVKDTNPPDLKVEPLYSQLSSERQSEIFK---INTSG---IRRCIVATNIAETSVTLDGI 1958
            S++   I  +R  LP++  + + ++++ +NQ+IV+VGETGSGKTTQ+ Q+ +E   +N      +GCTQPRRVAA+SVAKRVADE+  +LG++VGY IRFED  S NT++KY+TDG+LLRE++ D  L  Y VI++DEAHERTL TDIL GLL+EI + RSDL L+V SAT+DAEKF N+FG   + KIPGRTFPV++ +    + D V AAV      H   D  +GDIL+F++GQE+++  C +I+  +  + D    D+   PLYS L    Q  IF+    NT+     R+ +VATNIAETS+T+DG+
Sbjct:   34 SQKYYDILKKRVELPVWDYKEEFMQILSDNQIIVLVGETGSGKTTQIPQWCVEWVSDNSSEFKCVGCTQPRRVAAMSVAKRVADELDVELGEEVGYCIRFEDYTSPNTILKYLTDGMLLREAMSDPALKNYQVILLDEAHERTLGTDILMGLLKEISENRSDLKLIVMSATLDAEKFQNYFGNAPLMKIPGRTFPVEIFFTSEPVEDYVDAAVTVITLIHTLED--EGDILLFLTGQEEIDEACRRIEIEISNLGD-QVGDVSCIPLYSSLPPNVQQRIFEEAPPNTADGKISRKIVVATNIAETSITIDGV 342          
The following BLAST results are available for this feature:
BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
DHX380.000e+049.09DEAH-box helicase 38 [Source:HGNC Symbol;Acc:HGNC:... [more]
DHX167.796e-15745.43DEAH-box helicase 16 [Source:HGNC Symbol;Acc:HGNC:... [more]
DHX169.362e-15745.43DEAH-box helicase 16 [Source:HGNC Symbol;Acc:HGNC:... [more]
DHX169.362e-15745.43DEAH-box helicase 16 [Source:HGNC Symbol;Acc:HGNC:... [more]
DHX169.362e-15745.43DEAH-box helicase 16 [Source:HGNC Symbol;Acc:HGNC:... [more]
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BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
mog-10.000e+062.80Probable pre-mRNA-splicing factor ATP-dependent RN... [more]
mog-10.000e+062.80Probable pre-mRNA-splicing factor ATP-dependent RN... [more]
ddx-156.519e-13244.48Pre-mRNA-splicing factor ATP-dependent RNA helicas... [more]
ddx-151.607e-12844.48Pre-mRNA-splicing factor ATP-dependent RNA helicas... [more]
ddx-352.717e-11746.43Probable ATP-dependent RNA helicase DHX35 homolog ... [more]
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BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
l(1)G00070.000e+052.43gene:FBgn0026713 transcript:FBtr0073900[more]
l(1)G00074.566e-16862.76gene:FBgn0026713 transcript:FBtr0073901[more]
l(2)37Cb4.986e-15344.55gene:FBgn0086444 transcript:FBtr0081163[more]
Dhx152.262e-13741.69gene:FBgn0033160 transcript:FBtr0336909[more]
Dhx152.262e-13741.69gene:FBgn0033160 transcript:FBtr0088966[more]
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BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
dhx381.303e-15746.71DEAH (Asp-Glu-Ala-His) box polypeptide 38 [Source:... [more]
dhx381.303e-15746.71DEAH (Asp-Glu-Ala-His) box polypeptide 38 [Source:... [more]
dhx151.661e-12838.48DEAH (Asp-Glu-Ala-His) box helicase 15 [Source:ZFI... [more]
dhx331.559e-11638.13DEAH (Asp-Glu-Ala-His) box polypeptide 33 [Source:... [more]
AL833789.32.568e-9046.75DEAH (Asp-Glu-Ala-His) box polypeptide 8 [Source:N... [more]
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BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
DHX380.000e+047.94DEAH-box helicase 38 [Source:NCBI gene;Acc:734019][more]
DHX380.000e+047.63DEAH-box helicase 38 [Source:NCBI gene;Acc:734019][more]
DHX380.000e+047.55DEAH-box helicase 38 [Source:NCBI gene;Acc:734019][more]
DHX380.000e+047.55DEAH-box helicase 38 [Source:NCBI gene;Acc:734019][more]
DHX380.000e+047.63DEAH-box helicase 38 [Source:NCBI gene;Acc:734019][more]
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BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Dhx380.000e+049.67DEAH (Asp-Glu-Ala-His) box polypeptide 38 [Source:... [more]
Dhx161.326e-15645.73DEAH (Asp-Glu-Ala-His) box polypeptide 16 [Source:... [more]
Dhx156.506e-13039.76DEAH (Asp-Glu-Ala-His) box polypeptide 15 [Source:... [more]
Dhx337.141e-12139.38DEAH (Asp-Glu-Ala-His) box polypeptide 33 [Source:... [more]
Dhx151.129e-11846.89DEAH (Asp-Glu-Ala-His) box polypeptide 15 [Source:... [more]
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BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q92620|PRP16_HUMAN0.000e+049.09Pre-mRNA-splicing factor ATP-dependent RNA helicas... [more]
sp|Q17R09|PRP16_BOVIN0.000e+049.17Pre-mRNA-splicing factor ATP-dependent RNA helicas... [more]
sp|P34498|MOG1_CAEEL0.000e+062.80Probable pre-mRNA-splicing factor ATP-dependent RN... [more]
sp|F4K2E9|PRP16_ARATH0.000e+054.03Pre-mRNA-splicing factor ATP-dependent RNA helicas... [more]
sp|Q9P774|PRP16_SCHPO0.000e+054.77Pre-mRNA-splicing factor ATP-dependent RNA helicas... [more]
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BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0R3UJK80.000e+048.42Uncharacterized protein OS=Mesocestoides corti OX=... [more]
A0A5K3F2620.000e+048.42Uncharacterized protein OS=Mesocestoides corti OX=... [more]
A0A1W4X3L20.000e+052.95pre-mRNA-splicing factor ATP-dependent RNA helicas... [more]
A0A3Q3ACP30.000e+049.35DEAH (Asp-Glu-Ala-His) box polypeptide 38 OS=Krypt... [more]
A0A3Q2DLV60.000e+049.02DEAH (Asp-Glu-Ala-His) box polypeptide 38 OS=Cypri... [more]
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BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
dhx382.885e-14960.75DEAH (Asp-Glu-Ala-His) box polypeptide 38 [Source:... [more]
dhx156.728e-12837.90DEAH-box helicase 15 [Source:NCBI gene;Acc:1030385... [more]
dhx151.921e-12545.95DEAH-box helicase 15 [Source:NCBI gene;Acc:1030385... [more]
dhx152.020e-12545.95DEAH-box helicase 15 [Source:NCBI gene;Acc:1030385... [more]
dhx331.329e-12038.44DEAH (Asp-Glu-Ala-His) box polypeptide 33 [Source:... [more]
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BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
dhx88.297e-9045.27DEAH (Asp-Glu-Ala-His) box polypeptide 8 [Source:Z... [more]
dhx348.932e-8539.01DEAH (Asp-Glu-Ala-His) box polypeptide 34 [Source:... [more]
zgc:1588283.502e-7629.57DEAH-box helicase 35 [Source:HGNC Symbol;Acc:HGNC:... [more]
dqx12.118e-5530.82DEAQ box RNA-dependent ATPase 1 [Source:ZFIN;Acc:Z... [more]
dhx331.139e-4532.98DEAH (Asp-Glu-Ala-His) box polypeptide 33 [Source:... [more]
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BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 5
Match NameE-valueIdentityDescription
PRP167.773e-17951.14DEAH-box RNA helicase involved in second catalytic... [more]
PRP223.391e-16446.18DEAH-box RNA-dependent ATPase/ATP-dependent RNA he... [more]
PRP431.511e-12839.51RNA helicase in the DEAH-box family; functions in ... [more]
PRP21.558e-12149.36RNA-dependent DExD/H-box ATPase; required for acti... [more]
DHR22.018e-8843.83Predominantly nucleolar DEAH-box ATP-dependent RNA... [more]
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BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO356227.536e-14450.49Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO356213.487e-11462.26Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO495604.299e-9144.67Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO325315.543e-7140.73Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO326293.300e-6145.78Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
dhx380.000e+047.59DEAH-box helicase 38 [Source:NCBI gene;Acc:1011555... [more]
dhx154.702e-13146.93pre-mRNA-splicing factor ATP-dependent RNA helicas... [more]
dhx155.153e-13146.93pre-mRNA-splicing factor ATP-dependent RNA helicas... [more]
DHX153.296e-13037.90DEAH-box helicase 15 [Source:NCBI gene;Acc:1053574... [more]
dhx333.096e-11837.81DEAH-box helicase 33 [Source:NCBI gene;Acc:1011708... [more]
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BLAST of Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000023160.10.000e+0100.00SMESG000023160.1[more]
SMESG000010022.10.000e+076.03SMESG000010022.1[more]
SMESG000010011.10.000e+083.20SMESG000010011.1[more]
SMESG000006773.19.783e-15647.13SMESG000006773.1[more]
SMESG000009126.14.826e-14141.09SMESG000009126.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30028341 ID=SMED30028341|Name=Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16|organism=Schmidtea mediterranea sexual|type=transcript|length=3124bp
GTTTGTTTAAAAACACTTGCACTTTAAGCTATTTTTCAAATTTTAATTTA
ATGAATTCTGAATGTCGTAAATTATCAAAGAGAAATTATAGATCACGAGA
TGATCCAACTCCAATAAATGTAAAACTCGATAATGAGGAATATATTAAGC
GTCAAAACGAGAGAAAACAGCGTGATGAAAAACGAAAAATTGGTGAGTCT
TTCGGTTCTAAATCCAAAATAAAAAAAATTGAAAACATCGATCGTCATAA
GCAATGGGAAGAAATGACTCCCTCCACTTCTTTGCGTGATGAAACAGGTC
GTTATTCTGAAATTAGAACTCCAATGATTGATAGATCAATAAAAACGCCT
TCTGTAACAAGTTGGGATGAAGATGACGTAACTTTTAAAAGGCCTGATAC
AGTTACGAGTTTTCGTAGAAATGATAAATCAGAAATTAAATGGTCACCTG
ATAAAAATGAAGAAGAGTTAGACCGGGAATGGTATTTGAATGATGGAATG
GAGTATAACCCTTTTCCAAATTTTTCCGATGAAGTTTTAGAGAAGAAAAA
ACAACAGCTACAGAGACAAACGAGTATTGTTGCAAAATCTCGAAAATCTG
CTAGAGCAAAGCAATTTGAAGAAACTAATCGAGCTTGGGAAGAGAGTAAG
TTGATAAATTCAGGAGCTGTTGTAAGAAAAGGTTTTAACGTTGAAATTGA
TAATGAAGACACAGGCGAAGATAGAATTCATCTACATGTGCACAACATAT
TACCACCATTTCTAGATGGAAGAATAGTATTCACAAAGCAATTTGAACCT
GTAATACCGGTCAAAGATGAAAACTCAGATATGGCCAAAATAGCAAAAAA
TGGCAGTCAAATGGTTAAAAATTATAGAGATATTAAAGAAAAAATGAAAT
TACAGAAAAGAACTTTAGATATGACCGGTACAAATTTTGGCAAATTGATG
GGCTTAGAAAAAAAAGAAACTCTTGATAAATGGGATGAAACGAATTATAA
AGATTCAATGAAGTATTGTGATAAAATCACTGATAAAATTGCGCTTGATA
GTGAGCAGAAATCAAAAATAGATGATCAACGCAAACGATTACCGATATTT
AAGGTTCGCAGAGACTTGATTCGTTTGATTAGAGAAAATCAGGTTATCGT
GATTGTAGGAGAAACTGGCAGTGGGAAAACTACTCAATTAACTCAATATC
TGCTTGAAGAAAACTATCATCACAAAGGAATAATAGGCTGTACGCAACCG
AGGCGAGTTGCTGCAGTTTCAGTTGCCAAAAGAGTTGCCGATGAAGTCGG
CACAAAATTGGGCGATAAAGTCGGTTACTCAATTCGATTTGAAGATTGCA
AAAGTGAAAACACAATGATAAAATACATGACCGATGGTATTCTGTTGAGA
GAAAGTCTCCGAGATGAAGATCTCGATGCATATTCTGTGATAATAATGGA
CGAGGCTCACGAACGAACTCTCAACACCGATATATTGTTCGGGTTGCTTC
GAGAAATCATCCAACGAAGATCCGATCTCCATCTCGTAGTCACATCGGCA
ACAATGGACGCTGAGAAATTCTCGAACTTTTTCGGCAAATGTGAAATCTT
CAAAATCCCTGGCCGAACGTTCCCGGTCGATGTGATCTATGAGAAATCGA
GCATCCTCGATCCGGTAGCGGCTGCAGTGAACAAGGCGGTCGACACGCAT
TTGGGAGCTGACTCAAAGGACGGCGACATTCTCGTGTTTATGAGTGGCCA
GGAGGATGTCGAGGTGACTTGTGATAAAATCGATGAAACTCTAGAAAAAG
TCAAAGATACGAATCCGCCGGATTTGAAAGTCGAGCCGCTGTATTCGCAA
TTGTCGAGTGAAAGGCAATCGGAAATATTCAAAATCAATACTAGCGGGAT
TCGTCGGTGTATTGTTGCTACGAATATCGCTGAAACCTCTGTCACCCTCG
ACGGGATACGGAATGGACTCGTTGCAGATATATCCCATCAGTCAAGCCAG
TGCTAATCAAAGAGCCGGGCGGGCCGGGAGAACTGGGCCTGGCATCTGTT
ACCGGCTATACACGATCAATCAATACGAGAACGACATGCTCCCGATGACG
GTGCCGGAAATCCAGCGGACAAATCTCACAAATGTCGTGTTGTTATTGAA
ATCTCTCAACGTGCAAGATCTGATGAAATTCTATTTCATGGATTCTCCTC
CTCAGGATAACATTCTCAACTCCATGTACCATTTGTGGATGTTGGGGGCA
CTCGACGCCGTTGGCAATTTGACGGAACTCGGTCGGACTATGTCGGAATT
CCCTCTTGATCCCTCGCTATCGAAAATGTTGATAACATCTGTGAAACACG
GCTGTTCCGAGGAGATGTTGACGATCGTGTCGATGTTGAGCGTTCCGGCG
ATATTTTCCCGGCCTACTGGGCATCAAGAGGAATCTGATTGCGCACACGA
AAAGTTCCAAGTCGCTGAAAGTGATCATTTGACTCTGTTGAATGTCTATC
GGTTGTGGAGAGAGTCCGGATACTCGGCGAATTTCTGCAAAAAATACTAT
ATTCACAACAAATCGATGAAACGTGTCCGAGAAGTCCGGCAGCAATTGAA
GGAAATTATGATCAAATTGAAAATGAGTCTCGCATCGTCCGGGGATTTCG
ATATGATTCGGTATTGCATTTGTTCGGCGTATTTTAATCAAGCTGCACGG
TGGAAAGGTCTCGGAGAATACGTTAATCTACGCAGTGGAGTCGTTTGCCA
TCTCCATCCAACCAGTTCTCTTTATAATATGGCTTTCAATGCCGACTATG
TTGTCTATCATGAACTGTTGATGACGAGCAAGGAGTTCATGAACTATGTG
ACATTTGTCGATGCTCGGTGGCTGGCGGAATTGGGGCATATGTTTTATAG
TGTTAAGGAGAAAGGCCAATCGGTGACGGATAGAATGTTGAAGGAACAGC
AAACTGTTGAGAGAATGGAAACGGAAATGAGAATTAATGAAAAGGCTAAT
TTAAAGAAATTGTTGGATAACCGAGTTATCGAATCAGAAAGAAGGGCTCA
GAGTGTGAAGGAAACTACTTATTCACGGCCGAGATCGAGTAGATATGGAA
TATGATTTTGTACATTTAATAAAT
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protein sequence of SMED30028341-orf-1

>SMED30028341-orf-1 ID=SMED30028341-orf-1|Name=SMED30028341-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=381bp
MDSLQIYPISQASANQRAGRAGRTGPGICYRLYTINQYENDMLPMTVPEI
QRTNLTNVVLLLKSLNVQDLMKFYFMDSPPQDNILNSMYHLWMLGALDAV
GNLTELGRTMSEFPLDPSLSKMLITSVKHGCSEEMLTIVSMLSVPAIFSR
PTGHQEESDCAHEKFQVAESDHLTLLNVYRLWRESGYSANFCKKYYIHNK
SMKRVREVRQQLKEIMIKLKMSLASSGDFDMIRYCICSAYFNQAARWKGL
GEYVNLRSGVVCHLHPTSSLYNMAFNADYVVYHELLMTSKEFMNYVTFVD
ARWLAELGHMFYSVKEKGQSVTDRMLKEQQTVERMETEMRINEKANLKKL
LDNRVIESERRAQSVKETTYSRPRSSRYGI*
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protein sequence of SMED30028341-orf-2

>SMED30028341-orf-2 ID=SMED30028341-orf-2|Name=SMED30028341-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=668bp
LFKNTCTLSYFSNFNLMNSECRKLSKRNYRSRDDPTPINVKLDNEEYIKR
QNERKQRDEKRKIGESFGSKSKIKKIENIDRHKQWEEMTPSTSLRDETGR
YSEIRTPMIDRSIKTPSVTSWDEDDVTFKRPDTVTSFRRNDKSEIKWSPD
KNEEELDREWYLNDGMEYNPFPNFSDEVLEKKKQQLQRQTSIVAKSRKSA
RAKQFEETNRAWEESKLINSGAVVRKGFNVEIDNEDTGEDRIHLHVHNIL
PPFLDGRIVFTKQFEPVIPVKDENSDMAKIAKNGSQMVKNYRDIKEKMKL
QKRTLDMTGTNFGKLMGLEKKETLDKWDETNYKDSMKYCDKITDKIALDS
EQKSKIDDQRKRLPIFKVRRDLIRLIRENQVIVIVGETGSGKTTQLTQYL
LEENYHHKGIIGCTQPRRVAAVSVAKRVADEVGTKLGDKVGYSIRFEDCK
SENTMIKYMTDGILLRESLRDEDLDAYSVIIMDEAHERTLNTDILFGLLR
EIIQRRSDLHLVVTSATMDAEKFSNFFGKCEIFKIPGRTFPVDVIYEKSS
ILDPVAAAVNKAVDTHLGADSKDGDILVFMSGQEDVEVTCDKIDETLEKV
KDTNPPDLKVEPLYSQLSSERQSEIFKINTSGIRRCIVATNIAETSVTLD
GIRNGLVADISHQSSQC*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000429neoblast
Vocabulary: INTERPRO
TermDefinition
IPR007502Helicase-assoc_dom
IPR011709DUF1605
IPR027417P-loop_NTPase
IPR014001Helicase_ATP-bd
IPR011545DEAD/DEAH_box_helicase_dom
IPR001650Helicase_C
IPR002464DNA/RNA_helicase_DEAH_CS
IPR011528NERD
Vocabulary: molecular function
TermDefinition
GO:0004386helicase activity
GO:0003676nucleic acid binding
GO:0005524ATP binding
GO:0008026ATP-dependent helicase activity
GO:0000166nucleotide binding
GO:0016787hydrolase activity
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR014001Helicase superfamily 1/2, ATP-binding domainSMARTSM00487ultradead3coord: 361..545
e-value: 2.4E-32
score: 123.4
IPR014001Helicase superfamily 1/2, ATP-binding domainPROSITEPS51192HELICASE_ATP_BIND_1coord: 373..536
score: 20.399
NoneNo IPR availableGENE3DG3DSA:3.40.50.300coord: 314..538
e-value: 1.1E-89
score: 301.5
NoneNo IPR availableGENE3DG3DSA:3.40.50.300coord: 539..665
e-value: 7.6E-30
score: 105.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 46..67
NoneNo IPR availablePANTHERPTHR18934ATP-DEPENDENT RNA HELICASEcoord: 261..653
NoneNo IPR availablePANTHERPTHR18934:SF85ATP-DEPENDENT RNA HELICASE DHX8coord: 261..653
NoneNo IPR availableCDDcd17983DEXHc_DHX38coord: 363..535
e-value: 4.1783E-107
score: 319.793
NoneNo IPR availableCDDcd18791SF2_C_RHAcoord: 540..653
e-value: 3.49457E-29
score: 111.856
IPR011545DEAD/DEAH box helicase domainPFAMPF00270DEADcoord: 368..521
e-value: 7.5E-8
score: 32.3
IPR001650Helicase, C-terminalPFAMPF00271Helicase_Ccoord: 570..659
e-value: 2.2E-6
score: 28.0
IPR001650Helicase, C-terminalPROSITEPS51194HELICASE_CTERcoord: 562..667
score: 8.871
IPR002464DNA/RNA helicase, ATP-dependent, DEAH-box type, conserved sitePROSITEPS00690DEAH_ATP_HELICASEcoord: 478..487
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILYSSF52540P-loop containing nucleoside triphosphate hydrolasescoord: 357..654