DNA excision repair protein ERCC-1

Overview
NameDNA excision repair protein ERCC-1
Smed IDSMED30028315
Length (bp)982
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of DNA excision repair protein ERCC-1 (SMED30028315) t-SNE clustered cells

Violin plots show distribution of expression levels for DNA excision repair protein ERCC-1 (SMED30028315) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of DNA excision repair protein ERCC-1 (SMED30028315) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for DNA excision repair protein ERCC-1 (SMED30028315) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30028315

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 6

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
pigment cellSMED30028315h1SMcG0016384 SmedASXL_014426SmedAsxl_ww_GCZZ01PMID:29158443
He et al., 2017		whole organism asexual adult RNA-sequencing evidence
X1 cellSMED30028315h1SMcG0016384 SmedASXL_014426SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30028315h1SMcG0016384 SmedASXL_014426SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
Smed sexual biotypeSMED30028315h1SMcG0016384 Contig10876newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30028315h1SMcG0016384 Contig10876uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
zeta neoblastSMED30028315h1SMcG0016384 dd_Smed_v4_9619_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of DNA excision repair protein ERCC-1 vs. Ensembl Human
Match: ERCC1 (ERCC excision repair 1, endonuclease non-catalytic subunit [Source:HGNC Symbol;Acc:HGNC:3433])

HSP 1 Score: 158.688 bits (400), Expect = 1.106e-45
Identity = 99/257 (38.52%), Postives = 151/257 (58.75%), Query Frame = 1 
Query:  127 NIPSSQEQRPITYKTVSISPPNEFP--------ENAASTSLNTA--PIISSN-LIVNHRQKGNGVIDCLRSVRWAFGEIEPDFIMSRTSCALFLSLSYHKVKPSYIFTRIKGIGSLYELRXXXXXXXXXXSTQEIRELSQLCISNNLTLLLAWSNDEAARYLESFKMFENKPPDILTGEKIKSEKLSAAEEEINKYVSFLTSIASINKTDARKLMETFGVLLIPAEFIYCLSQTIEAIMLSDFEQLRSCPGLGALKV 864
             + +S +  P TY   +IS P E          E  A  + N A  P   SN +IV+ RQ+GN V+  +R+V W FG++ PD+++ +++CALFLSL YH + P YI  R++ +G  + LRVLLV VDV D  Q ++EL+++CI  + TL+LAWS +EA RYLE++K +E KP D+L       EKL   ++ +++    LT++ S+NKTD++ L+ TFG              ++E ++ +  E L  CPGLG  K 
Sbjct:   48 TVDTSAQAAPQTYAEYAISQPLEGAGATCPTGSEPLAGETPNQALKPGAKSNSIIVSPRQRGNPVLKFVRNVPWEFGDVIPDYVLGQSTCALFLSLRYHNLHPDYIHGRLQSLGKNFALRVLLVQVDVKDPQQALKELAKMCILADCTLILAWSPEEAGRYLETYKAYEQKPADLLM------EKLE--QDFVSRVTECLTTVKSVNKTDSQTLLTTFG--------------SLEQLIAASREDLALCPGLGPQKA 282
[Close]
BLAST of DNA excision repair protein ERCC-1 vs. Ensembl Human
Match: ERCC1 (ERCC excision repair 1, endonuclease non-catalytic subunit [Source:HGNC Symbol;Acc:HGNC:3433])

HSP 1 Score: 158.688 bits (400), Expect = 1.106e-45
Identity = 99/257 (38.52%), Postives = 151/257 (58.75%), Query Frame = 1 
Query:  127 NIPSSQEQRPITYKTVSISPPNEFP--------ENAASTSLNTA--PIISSN-LIVNHRQKGNGVIDCLRSVRWAFGEIEPDFIMSRTSCALFLSLSYHKVKPSYIFTRIKGIGSLYELRXXXXXXXXXXSTQEIRELSQLCISNNLTLLLAWSNDEAARYLESFKMFENKPPDILTGEKIKSEKLSAAEEEINKYVSFLTSIASINKTDARKLMETFGVLLIPAEFIYCLSQTIEAIMLSDFEQLRSCPGLGALKV 864
             + +S +  P TY   +IS P E          E  A  + N A  P   SN +IV+ RQ+GN V+  +R+V W FG++ PD+++ +++CALFLSL YH + P YI  R++ +G  + LRVLLV VDV D  Q ++EL+++CI  + TL+LAWS +EA RYLE++K +E KP D+L       EKL   ++ +++    LT++ S+NKTD++ L+ TFG              ++E ++ +  E L  CPGLG  K 
Sbjct:   48 TVDTSAQAAPQTYAEYAISQPLEGAGATCPTGSEPLAGETPNQALKPGAKSNSIIVSPRQRGNPVLKFVRNVPWEFGDVIPDYVLGQSTCALFLSLRYHNLHPDYIHGRLQSLGKNFALRVLLVQVDVKDPQQALKELAKMCILADCTLILAWSPEEAGRYLETYKAYEQKPADLLM------EKLE--QDFVSRVTECLTTVKSVNKTDSQTLLTTFG--------------SLEQLIAASREDLALCPGLGPQKA 282
[Close]
BLAST of DNA excision repair protein ERCC-1 vs. Ensembl Human
Match: ERCC1 (ERCC excision repair 1, endonuclease non-catalytic subunit [Source:HGNC Symbol;Acc:HGNC:3433])

HSP 1 Score: 159.073 bits (401), Expect = 1.239e-45
Identity = 100/256 (39.06%), Postives = 152/256 (59.38%), Query Frame = 1 
Query:  130 IPSSQEQRPITYKTVSISPPNEFP--------ENAASTSLNTA--PIISSN-LIVNHRQKGNGVIDCLRSVRWAFGEIEPDFIMSRTSCALFLSLSYHKVKPSYIFTRIKGIGSLYELRXXXXXXXXXXSTQEIRELSQLCISNNLTLLLAWSNDEAARYLESFKMFENKPPDILTGEKIKSEKLSAAEEEINKYVSFLTSIASINKTDARKLMETFGVLLIPAEFIYCLSQTIEAIMLSDFEQLRSCPGLGALKV 864
            + +S +  P TY   +IS P E          E  A  + N A  P   SN +IV+ RQ+GN V+  +R+V W FG++ PD+++ +++CALFLSL YH + P YI  R++ +G  + LRVLLV VDV D  Q ++EL+++CI  + TL+LAWS +EA RYLE++K +E KP D+L       EKL   ++ +++    LT++ S+NKTD++ L+ TFG              ++E ++ +  E L  CPGLG  KV
Sbjct:   49 VDTSAQAAPQTYAEYAISQPLEGAGATCPTGSEPLAGETPNQALKPGAKSNSIIVSPRQRGNPVLKFVRNVPWEFGDVIPDYVLGQSTCALFLSLRYHNLHPDYIHGRLQSLGKNFALRVLLVQVDVKDPQQALKELAKMCILADCTLILAWSPEEAGRYLETYKAYEQKPADLLM------EKLE--QDFVSRVTECLTTVKSVNKTDSQTLLTTFG--------------SLEQLIAASREDLALCPGLGPQKV 282
[Close]
BLAST of DNA excision repair protein ERCC-1 vs. Ensembl Human
Match: ERCC1 (ERCC excision repair 1, endonuclease non-catalytic subunit [Source:HGNC Symbol;Acc:HGNC:3433])

HSP 1 Score: 149.828 bits (377), Expect = 7.988e-43
Identity = 90/219 (41.10%), Postives = 137/219 (62.56%), Query Frame = 1 
Query:  127 NIPSSQEQRPITYKTVSISPPNEFP--------ENAASTSLNTA--PIISSN-LIVNHRQKGNGVIDCLRSVRWAFGEIEPDFIMSRTSCALFLSLSYHKVKPSYIFTRIKGIGSLYELRXXXXXXXXXXSTQEIRELSQLCISNNLTLLLAWSNDEAARYLESFKMFENKPPDILTGEKIKSEKLSAAEEEINKYVSFLTSIASINKTDARKLMETFG 750
             + +S +  P TY   +IS P E          E  A  + N A  P   SN +IV+ RQ+GN V+  +R+V W FG++ PD+++ +++CALFLSL YH + P YI  R++ +G  + LRVLLV VDV D  Q ++EL+++CI  + TL+LAWS +EA RYLE++K +E KP D+L       EKL   ++ +++    LT++ S+NKTD++ L+ TFG
Sbjct:   48 TVDTSAQAAPQTYAEYAISQPLEGAGATCPTGSEPLAGETPNQALKPGAKSNSIIVSPRQRGNPVLKFVRNVPWEFGDVIPDYVLGQSTCALFLSLRYHNLHPDYIHGRLQSLGKNFALRVLLVQVDVKDPQQALKELAKMCILADCTLILAWSPEEAGRYLETYKAYEQKPADLLM------EKLE--QDFVSRVTECLTTVKSVNKTDSQTLLTTFG 258
[Close]
BLAST of DNA excision repair protein ERCC-1 vs. Ensembl Human
Match: ERCC1 (ERCC excision repair 1, endonuclease non-catalytic subunit [Source:HGNC Symbol;Acc:HGNC:3433])

HSP 1 Score: 146.362 bits (368), Expect = 4.471e-42
Identity = 80/196 (40.82%), Postives = 125/196 (63.78%), Query Frame = 1 
Query:  274 KGNGVIDCLRSVRWAFGEIEPDFIMSRTSCALFLSLSYHKVKPSYIFTRIKGIGSLYELRXXXXXXXXXXSTQEIRELSQLCISNNLTLLLAWSNDEAARYLESFKMFENKPPDILTGEKIKSEKLSAAEEEINKYVSFLTSIASINKTDARKLMETFGVLLIPAEFIYCLSQTIEAIMLSDFEQLRSCPGLGALK 861
            +GN V+  +R+V W FG++ PD+++ +++CALFLSL YH + P YI  R++ +G  + LRVLLV VDV D  Q ++EL+++CI  + TL+LAWS +EA RYLE++K +E KP D+L       EKL   ++ +++    LT++ S+NKTD++ L+ TFG              ++E ++ +  E L  CPGLG  K
Sbjct:   36 RGNPVLKFVRNVPWEFGDVIPDYVLGQSTCALFLSLRYHNLHPDYIHGRLQSLGKNFALRVLLVQVDVKDPQQALKELAKMCILADCTLILAWSPEEAGRYLETYKAYEQKPADLLM------EKLE--QDFVSRVTECLTTVKSVNKTDSQTLLTTFG--------------SLEQLIAASREDLALCPGLGPQK 209
[Close]
BLAST of DNA excision repair protein ERCC-1 vs. Ensembl Celegans
Match: ercc-1 (ERCC (DNA excision repair protein) homolog [Source:UniProtKB/TrEMBL;Acc:Q93456])

HSP 1 Score: 99.3673 bits (246), Expect = 4.603e-24
Identity = 70/213 (32.86%), Postives = 112/213 (52.58%), Query Frame = 1 
Query:  244 SSNLIVNHR-QKGNGVIDCLRSVRWAFGEIEPDFIMSRTSCALFLSLSYHKVKPSYIFTRIKGIG-SLYELRXXXXXXXXXXSTQEIRELSQLCISNNLTLLLAWSNDEAARYLESFKMFENKPPDILTGEKIKSEKLSAAEEEINK----YVSFLTSIASINKTDARKLMETFGVLLIPAEFIYCLSQTIEAIMLSDFEQLRSCPGLGALKV 864
            S  L+VN R Q+GN V+  +R+VR+ +G+I PDF    T   ++LS  YHK  P Y++TRI G   + Y  +VLL   ++ +    +REL+ +C     T ++ ++ +EAA Y+E FK  + K   I   + I     S+  +E  +     + FLT+  SI KTDA +L+  FG              T++AI  +    + +CPG+G +K 
Sbjct:   48 SGALVVNRRRQEGNPVLKYVRNVRYEWGDIGPDFECGPTFGVVYLSFKYHKQHPEYVYTRINGNAENRYRNKVLLGYCNMEEPRHVLRELNMICFREAWTFVVVYTVEEAAEYIELFKTTQKKEITI-KKKAIDDGGDSSMSDERRRNREAAIGFLTAARSITKTDADRLLFHFG--------------TLQAISTASETSISACPGVGPIKA 245
[Close]
BLAST of DNA excision repair protein ERCC-1 vs. Ensembl Celegans
Match: ercc-1 (ERCC (DNA excision repair protein) homolog [Source:UniProtKB/TrEMBL;Acc:Q93456])

HSP 1 Score: 99.3673 bits (246), Expect = 4.603e-24
Identity = 70/213 (32.86%), Postives = 112/213 (52.58%), Query Frame = 1 
Query:  244 SSNLIVNHR-QKGNGVIDCLRSVRWAFGEIEPDFIMSRTSCALFLSLSYHKVKPSYIFTRIKGIG-SLYELRXXXXXXXXXXSTQEIRELSQLCISNNLTLLLAWSNDEAARYLESFKMFENKPPDILTGEKIKSEKLSAAEEEINK----YVSFLTSIASINKTDARKLMETFGVLLIPAEFIYCLSQTIEAIMLSDFEQLRSCPGLGALKV 864
            S  L+VN R Q+GN V+  +R+VR+ +G+I PDF    T   ++LS  YHK  P Y++TRI G   + Y  +VLL   ++ +    +REL+ +C     T ++ ++ +EAA Y+E FK  + K   I   + I     S+  +E  +     + FLT+  SI KTDA +L+  FG              T++AI  +    + +CPG+G +K 
Sbjct:   48 SGALVVNRRRQEGNPVLKYVRNVRYEWGDIGPDFECGPTFGVVYLSFKYHKQHPEYVYTRINGNAENRYRNKVLLGYCNMEEPRHVLRELNMICFREAWTFVVVYTVEEAAEYIELFKTTQKKEITI-KKKAIDDGGDSSMSDERRRNREAAIGFLTAARSITKTDADRLLFHFG--------------TLQAISTASETSISACPGVGPIKA 245
[Close]
BLAST of DNA excision repair protein ERCC-1 vs. Ensembl Fly
Match: Ercc1 (gene:FBgn0028434 transcript:FBtr0087448)

HSP 1 Score: 126.331 bits (316), Expect = 3.698e-34
Identity = 66/169 (39.05%), Postives = 111/169 (65.68%), Query Frame = 1 
Query:  253 LIVNHRQKGNGVIDCLRSVRWAF-GEIEPDFIMSRTSCALFLSLSYHKVKPSYIFTRIKGIGSLYELRXXXXXXXXXXSTQEIRELSQLCISNNLTLLLAWSNDEAARYLESFKMFENKPPDILTGEKIKSEKLSAAEEEINKYVSFLTSIASINKTDARKLMETFGVL 756
            ++V+ +Q+GN ++  + +V   F  +I PD+++ RTSC L+LSL YH + P YI  R+K +G +YELRVLLV VD  +    ++ L+++ +  +LT++LAW+ +EA + +E++K FE +PPD++  E+++S           K ++ LT+I  +NKTDA  L+ TFG L
Sbjct:   63 VLVHSKQRGNPILKSILNVPLEFRDDIVPDYVVGRTSCVLYLSLKYHNLNPDYICQRLKALGKMYELRVLLVQVDTPEPNNALKSLTRISLLADLTMMLAWNAEEAGKIIETYKQFEKRPPDLIM-ERVESNPHQ-------KLLAALTNIKPVNKTDAAALLHTFGNL 223
[Close]
BLAST of DNA excision repair protein ERCC-1 vs. Ensembl Zebrafish
Match: ercc1 (excision repair cross-complementation group 1 [Source:ZFIN;Acc:ZDB-GENE-040426-2606])

HSP 1 Score: 153.68 bits (387), Expect = 1.480e-43
Identity = 84/206 (40.78%), Postives = 131/206 (63.59%), Query Frame = 1 
Query:  247 SNLIVNHRQKGNGVIDCLRSVRWAFGEIEPDFIMSRTSCALFLSLSYHKVKPSYIFTRIKGIGSLYELRXXXXXXXXXXSTQEIRELSQLCISNNLTLLLAWSNDEAARYLESFKMFENKPPDILTGEKIKSEKLSAAEEEINKYVSFLTSIASINKTDARKLMETFGVLLIPAEFIYCLSQTIEAIMLSDFEQLRSCPGLGALKV 864
            +++IV+ RQ+GN ++  +R+V W FGE+ PD+++ RT+CALFLS+ YH + P+Y+  R+K +G  + LR+LLV VDV D    ++EL+++CI  + TL+LAWS +EA RYLE++K +E KP D+L  E+++   LS       +    LT++ S+NKTDA  L+ TF               ++E I+ +  E+L  CPGLG  K 
Sbjct:  140 NSIIVSPRQRGNPILKFVRNVPWEFGEVVPDYVLGRTTCALFLSVRYHNLNPNYVHERLKQLGQSFSLRILLVQVDVKDPHHALKELARICIMADCTLILAWSPEEAGRYLETYKSYEKKPADLLK-EQVEKNYLS-------QVTDCLTTVKSVNKTDAMTLLSTFS--------------SLEGIIKASKEELVLCPGLGPQKA 323
[Close]
BLAST of DNA excision repair protein ERCC-1 vs. Ensembl Xenopus
Match: ercc1 (excision repair cross-complementation group 1 [Source:NCBI gene;Acc:100135224])

HSP 1 Score: 147.517 bits (371), Expect = 9.522e-42
Identity = 90/220 (40.91%), Postives = 133/220 (60.45%), Query Frame = 1 
Query:  202 ENAASTSLNTAPIISSNLIVNHRQKGNGVIDCLRSVRWAFGEIEPDFIMSRTSCALFLSLSYHKVKPSYIFTRIKGIGSLYELRXXXXXXXXXXSTQEIRELSQLCISNNLTLLLAWSNDEAARYLESFKMFENKPPDILTGEKIKSEKLSAAEEEINKYVSFLTSIASINKTDARKLMETFGVLLIPAEFIYCLSQTIEAIMLSDFEQLRSCPGLGALK 861
            E AA+ +  +  + SS +IV+ RQ+GN ++  LR+V W FG I PD+++  T CALFLSL YH + P YI  R++ +G  + LRVLLV VDV D    ++EL+++CI ++ TL+L+WS +EAARYLE++K +E KP D+L   K ++EK     + +++    LT++  +NKTD+  L  TFG L   A               +  E L  CPGLG  K
Sbjct:   64 EQAANQTTKSVGVRSS-IIVSTRQRGNSLLKYLRNVPWEFGNIIPDYVLGETCCALFLSLRYHNLNPEYIHLRLRSLGQSFALRVLLVQVDVKDPHFSLKELAKICILSDCTLILSWSPEEAARYLETYKCYEQKPADVL---KERTEK-----DLMSRMAECLTTVKYVNKTDSCTLFTTFGTLFDLAN--------------ASREDLSLCPGLGPQK 260
[Close]
BLAST of DNA excision repair protein ERCC-1 vs. Ensembl Xenopus
Match: ercc1 (excision repair cross-complementation group 1 [Source:NCBI gene;Acc:100135224])

HSP 1 Score: 146.747 bits (369), Expect = 1.987e-41
Identity = 90/221 (40.72%), Postives = 133/221 (60.18%), Query Frame = 1 
Query:  202 ENAASTSLNTAPIISSNLIVNHRQKGNGVIDCLRSVRWAFGEIEPDFIMSRTSCALFLSLSYHKVKPSYIFTRIKGIGSLYELRXXXXXXXXXXSTQEIRELSQLCISNNLTLLLAWSNDEAARYLESFKMFENKPPDILTGEKIKSEKLSAAEEEINKYVSFLTSIASINKTDARKLMETFGVLLIPAEFIYCLSQTIEAIMLSDFEQLRSCPGLGALKV 864
            E AA+ +  +  + SS +IV+ RQ+GN ++  LR+V W FG I PD+++  T CALFLSL YH + P YI  R++ +G  + LRVLLV VDV D    ++EL+++CI ++ TL+L+WS +EAARYLE++K +E KP D+L   K ++EK     + +++    LT++  +NKTD+  L  TFG L   A               +  E L  CPGLG  K 
Sbjct:   65 EQAANQTTKSVGVRSS-IIVSTRQRGNSLLKYLRNVPWEFGNIIPDYVLGETCCALFLSLRYHNLNPEYIHLRLRSLGQSFALRVLLVQVDVKDPHFSLKELAKICILSDCTLILSWSPEEAARYLETYKCYEQKPADVL---KERTEK-----DLMSRMAECLTTVKYVNKTDSCTLFTTFGTLFDLAN--------------ASREDLSLCPGLGPQKA 262
[Close]
BLAST of DNA excision repair protein ERCC-1 vs. Ensembl Xenopus
Match: ercc1 (excision repair cross-complementation group 1 [Source:NCBI gene;Acc:100135224])

HSP 1 Score: 147.132 bits (370), Expect = 2.998e-41
Identity = 90/221 (40.72%), Postives = 133/221 (60.18%), Query Frame = 1 
Query:  202 ENAASTSLNTAPIISSNLIVNHRQKGNGVIDCLRSVRWAFGEIEPDFIMSRTSCALFLSLSYHKVKPSYIFTRIKGIGSLYELRXXXXXXXXXXSTQEIRELSQLCISNNLTLLLAWSNDEAARYLESFKMFENKPPDILTGEKIKSEKLSAAEEEINKYVSFLTSIASINKTDARKLMETFGVLLIPAEFIYCLSQTIEAIMLSDFEQLRSCPGLGALKV 864
            E AA+ +  +  + SS +IV+ RQ+GN ++  LR+V W FG I PD+++  T CALFLSL YH + P YI  R++ +G  + LRVLLV VDV D    ++EL+++CI ++ TL+L+WS +EAARYLE++K +E KP D+L   K ++EK     + +++    LT++  +NKTD+  L  TFG L   A               +  E L  CPGLG  K 
Sbjct:   85 EQAANQTTKSVGVRSS-IIVSTRQRGNSLLKYLRNVPWEFGNIIPDYVLGETCCALFLSLRYHNLNPEYIHLRLRSLGQSFALRVLLVQVDVKDPHFSLKELAKICILSDCTLILSWSPEEAARYLETYKCYEQKPADVL---KERTEK-----DLMSRMAECLTTVKYVNKTDSCTLFTTFGTLFDLAN--------------ASREDLSLCPGLGPQKA 282
[Close]
BLAST of DNA excision repair protein ERCC-1 vs. Ensembl Mouse
Match: Ercc1 (excision repair cross-complementing rodent repair deficiency, complementation group 1 [Source:MGI Symbol;Acc:MGI:95412])

HSP 1 Score: 159.073 bits (401), Expect = 5.725e-46
Identity = 89/207 (43.00%), Postives = 133/207 (64.25%), Query Frame = 1 
Query:  244 SSNLIVNHRQKGNGVIDCLRSVRWAFGEIEPDFIMSRTSCALFLSLSYHKVKPSYIFTRIKGIGSLYELRXXXXXXXXXXSTQEIRELSQLCISNNLTLLLAWSNDEAARYLESFKMFENKPPDILTGEKIKSEKLSAAEEEINKYVSFLTSIASINKTDARKLMETFGVLLIPAEFIYCLSQTIEAIMLSDFEQLRSCPGLGALKV 864
            S+++IV+ RQ+GN V+  +R+V W FGE+ PD+++ +++CALFLSL YH + P YI  R++ +G  + LRVLLV VDV D  Q ++EL+++CI  + TL+LAWS +EA RYLE++K +E KP D+L  EK++   LS A E        LT++ S+NKTD++ L+ TFG              ++E +  +  E L  CPGLG  K 
Sbjct:   98 SNSIIVSPRQRGNPVLKFVRNVPWEFGEVIPDYVLGQSTCALFLSLRYHNLHPDYIHERLQSLGKNFALRVLLVQVDVKDPQQALKELAKMCILADCTLVLAWSAEEAGRYLETYKAYEQKPADLLM-EKLEQNFLSRATE-------CLTTVKSVNKTDSQTLLATFG--------------SLEQLFTASREDLALCPGLGPQKA 282
[Close]
BLAST of DNA excision repair protein ERCC-1 vs. Ensembl Mouse
Match: Ercc1 (excision repair cross-complementing rodent repair deficiency, complementation group 1 [Source:MGI Symbol;Acc:MGI:95412])

HSP 1 Score: 130.954 bits (328), Expect = 8.046e-36
Identity = 67/137 (48.91%), Postives = 101/137 (73.72%), Query Frame = 1 
Query:  244 SSNLIVNHRQKGNGVIDCLRSVRWAFGEIEPDFIMSRTSCALFLSLSYHKVKPSYIFTRIKGIGSLYELRXXXXXXXXXXSTQEIRELSQLCISNNLTLLLAWSNDEAARYLESFKMFENKPPDILTGEKIKSEKLS 654
            S+++IV+ RQ+GN V+  +R+V W FGE+ PD+++ +++CALFLSL YH + P YI  R++ +G  + LRVLLV VDV D  Q ++EL+++CI  + TL+LAWS +EA RYLE++K +E KP D+L  EK++   LS
Sbjct:   98 SNSIIVSPRQRGNPVLKFVRNVPWEFGEVIPDYVLGQSTCALFLSLRYHNLHPDYIHERLQSLGKNFALRVLLVQVDVKDPQQALKELAKMCILADCTLVLAWSAEEAGRYLETYKAYEQKPADLLM-EKLEQNFLS 233
[Close]
BLAST of DNA excision repair protein ERCC-1 vs. Ensembl Mouse
Match: Ercc1 (excision repair cross-complementing rodent repair deficiency, complementation group 1 [Source:MGI Symbol;Acc:MGI:95412])

HSP 1 Score: 79.337 bits (194), Expect = 6.398e-18
Identity = 32/70 (45.71%), Postives = 52/70 (74.29%), Query Frame = 1 
Query:  244 SSNLIVNHRQKGNGVIDCLRSVRWAFGEIEPDFIMSRTSCALFLSLSYHKVKPSYIFTRIKGIGSLYELR 453
            S+++IV+ RQ+GN V+  +R+V W FGE+ PD+++ +++CALFLSL YH + P YI  R++ +G  + LR
Sbjct:   22 SNSIIVSPRQRGNPVLKFVRNVPWEFGEVIPDYVLGQSTCALFLSLRYHNLHPDYIHERLQSLGKNFALR 91
[Close]
BLAST of DNA excision repair protein ERCC-1 vs. UniProt/SwissProt
Match: sp|Q1LZ75|ERCC1_BOVIN (DNA excision repair protein ERCC-1 OS=Bos taurus OX=9913 GN=ERCC1 PE=2 SV=1)

HSP 1 Score: 164.466 bits (415), Expect = 2.243e-47
Identity = 100/275 (36.36%), Postives = 160/275 (58.18%), Query Frame = 1 
Query:   73 IIEKGGLFQMYKAGKIDCNIPSSQEQRPITYKTVSIS-PPNEF-------PENAASTSLNTAP---IISSNLIVNHRQKGNGVIDCLRSVRWAFGEIEPDFIMSRTSCALFLSLSYHKVKPSYIFTRIKGIGSLYELRXXXXXXXXXXSTQEIRELSQLCISNNLTLLLAWSNDEAARYLESFKMFENKPPDILTGEKIKSEKLSAAEEEINKYVSFLTSIASINKTDARKLMETFGVLLIPAEFIYCLSQTIEAIMLSDFEQLRSCPGLGALKV 864
            ++   G   ++++ +    + +S    P TY   ++S PP          PE  A  + N AP     S+++IV+ RQ+GN V+  +R+V W FG++ PD+++ +++CALFLSL YH + P YI  R++ +G  Y LRVLLV VDV D  Q ++EL+++CI  + TL+LAWS +EA RYLE++K +E KP D+L       EKL   ++ +++    LT++ S+NKTD++ L+ TFG              ++E ++ +  E L  CPGLG  K 
Sbjct:   26 VVPPPGAKPLFRSTRSLPTVETSPPPGPQTYAEYALSGPPGGAEATRPVGPEPLAEETPNQAPKPGAKSNSIIVSPRQRGNPVLRFVRNVPWEFGDVLPDYVLGQSTCALFLSLRYHNLHPDYIHQRLQSLGKSYALRVLLVQVDVKDPQQALKELAKMCILADCTLILAWSPEEAGRYLETYKAYEQKPADLLM------EKLE--QDFVSRVTECLTTVKSVNKTDSQTLLTTFG--------------SLEQLIAASREDLSLCPGLGPQKA 278
[Close]
BLAST of DNA excision repair protein ERCC-1 vs. UniProt/SwissProt
Match: sp|P07903|ERCC1_MOUSE (DNA excision repair protein ERCC-1 OS=Mus musculus OX=10090 GN=Ercc1 PE=2 SV=2)

HSP 1 Score: 159.073 bits (401), Expect = 4.005e-45
Identity = 89/207 (43.00%), Postives = 133/207 (64.25%), Query Frame = 1 
Query:  244 SSNLIVNHRQKGNGVIDCLRSVRWAFGEIEPDFIMSRTSCALFLSLSYHKVKPSYIFTRIKGIGSLYELRXXXXXXXXXXSTQEIRELSQLCISNNLTLLLAWSNDEAARYLESFKMFENKPPDILTGEKIKSEKLSAAEEEINKYVSFLTSIASINKTDARKLMETFGVLLIPAEFIYCLSQTIEAIMLSDFEQLRSCPGLGALKV 864
            S+++IV+ RQ+GN V+  +R+V W FGE+ PD+++ +++CALFLSL YH + P YI  R++ +G  + LRVLLV VDV D  Q ++EL+++CI  + TL+LAWS +EA RYLE++K +E KP D+L  EK++   LS A E        LT++ S+NKTD++ L+ TFG              ++E +  +  E L  CPGLG  K 
Sbjct:   98 SNSIIVSPRQRGNPVLKFVRNVPWEFGEVIPDYVLGQSTCALFLSLRYHNLHPDYIHERLQSLGKNFALRVLLVQVDVKDPQQALKELAKMCILADCTLVLAWSAEEAGRYLETYKAYEQKPADLLM-EKLEQNFLSRATE-------CLTTVKSVNKTDSQTLLATFG--------------SLEQLFTASREDLALCPGLGPQKA 282
[Close]
BLAST of DNA excision repair protein ERCC-1 vs. UniProt/SwissProt
Match: sp|P07992|ERCC1_HUMAN (DNA excision repair protein ERCC-1 OS=Homo sapiens OX=9606 GN=ERCC1 PE=1 SV=1)

HSP 1 Score: 158.688 bits (400), Expect = 5.313e-45
Identity = 99/257 (38.52%), Postives = 151/257 (58.75%), Query Frame = 1 
Query:  127 NIPSSQEQRPITYKTVSISPPNEFP--------ENAASTSLNTA--PIISSN-LIVNHRQKGNGVIDCLRSVRWAFGEIEPDFIMSRTSCALFLSLSYHKVKPSYIFTRIKGIGSLYELRXXXXXXXXXXSTQEIRELSQLCISNNLTLLLAWSNDEAARYLESFKMFENKPPDILTGEKIKSEKLSAAEEEINKYVSFLTSIASINKTDARKLMETFGVLLIPAEFIYCLSQTIEAIMLSDFEQLRSCPGLGALKV 864
             + +S +  P TY   +IS P E          E  A  + N A  P   SN +IV+ RQ+GN V+  +R+V W FG++ PD+++ +++CALFLSL YH + P YI  R++ +G  + LRVLLV VDV D  Q ++EL+++CI  + TL+LAWS +EA RYLE++K +E KP D+L       EKL   ++ +++    LT++ S+NKTD++ L+ TFG              ++E ++ +  E L  CPGLG  K 
Sbjct:   48 TVDTSAQAAPQTYAEYAISQPLEGAGATCPTGSEPLAGETPNQALKPGAKSNSIIVSPRQRGNPVLKFVRNVPWEFGDVIPDYVLGQSTCALFLSLRYHNLHPDYIHGRLQSLGKNFALRVLLVQVDVKDPQQALKELAKMCILADCTLILAWSPEEAGRYLETYKAYEQKPADLLM------EKLE--QDFVSRVTECLTTVKSVNKTDSQTLLTTFG--------------SLEQLIAASREDLALCPGLGPQKA 282
[Close]
BLAST of DNA excision repair protein ERCC-1 vs. UniProt/SwissProt
Match: sp|Q06182|SWI10_SCHPO (Mating-type switching protein swi10 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=swi10 PE=1 SV=1)

HSP 1 Score: 141.354 bits (355), Expect = 7.240e-39
Identity = 83/209 (39.71%), Postives = 126/209 (60.29%), Query Frame = 1 
Query:  238 IISSNLIVNHRQKGNGVIDCLRSVRWAFGEIEPDFIMSRTSCALFLSLSYHKVKPSYIFTRIKGIGSLYELRXXXXXXXXXXSTQEIRELSQLCISNNLTLLLAWSNDEAARYLESFKMFENKPPDILTGEKIKSEKLSAAEEEINKYVSFLTSIASINKTDARKLMETFGVLLIPAEFIYCLSQTIEAIMLSDFEQLRSCPGLGALKV 864
            + + +++VN RQKGN ++  +R+V W + +I PDF+M    C+LFLSL YH + P YI++RI  +G  Y LR+LL+LVDV +    I+EL +  I N  TL+LAWS++EAARYLE++K +EN  P ++  EK  ++ LS  +       SFLTSI  INK+D+  L+  FG              ++E  +++  ++L    G G  KV
Sbjct:   38 VTAHSILVNPRQKGNPLLPHVRNVPWEYTDIVPDFVMGTGICSLFLSLKYHHLHPEYIYSRISKLGKSYNLRILLILVDVENHQASIQELVKTSIVNQYTLILAWSSEEAARYLETYKAYENMSPALIM-EKPSTDYLSQVQ-------SFLTSIRGINKSDSLSLLSKFG--------------SLERALVASRDELEQLEGWGPTKV 224
[Close]
BLAST of DNA excision repair protein ERCC-1 vs. UniProt/SwissProt
Match: sp|Q9MA98|ERCC1_ARATH (DNA excision repair protein ERCC-1 OS=Arabidopsis thaliana OX=3702 GN=ERCC1 PE=2 SV=1)

HSP 1 Score: 137.502 bits (345), Expect = 6.303e-36
Identity = 83/217 (38.25%), Postives = 131/217 (60.37%), Query Frame = 1 
Query:  253 LIVNHRQKGNGVIDCLRSVRWAFGEIEPDFIMSRTSCALFLSLSYHKVKPSYIFTRIKGIGSLYELRXXXXXXXXXXSTQEIRELSQLCISNNLTLLLAWSNDEAARYLESFKMFENKPPDILTGEKIKSEKLSAAEEEINKYVSFLTSIASINKTDARKLMETFGVLLIPAEFIYCLSQTIEAIMLSDFEQLRSCPGLGALKVLLGLFFTFIIEFR 903
            ++V+HRQKGN ++  +R+V+W F +I PD+++ + SCAL+LSL YH + P Y++ RI+ +   ++L V+L  VDV D+ + + E+++  + ++ TLL AWS  E ARYLE+ K++ENKP D++ G+ + ++ LS       +    LTSI  +NK+D   L  TFG           L+  I+A M    E L  CPG+G  KV   L+ TF   F+
Sbjct:  126 ILVSHRQKGNPLLKHIRNVKWVFSDIIPDYVLGQNSCALYLSLRYHLLHPDYLYFRIRELQKNFKLSVVLCHVDVEDTVKPLLEVTKTALLHDCTLLCAWSMTECARYLETIKVYENKPADLIQGQ-MDTDYLS-------RLNHSLTSIRHVNKSDVVTLGSTFG----------SLAHIIDASM----EDLARCPGIGERKV-KRLYDTFHEPFK 319
[Close]
BLAST of DNA excision repair protein ERCC-1 vs. TrEMBL
Match: A0A397H2E6 (UPF0029 domain-containing protein OS=Diversispora epigaea OX=1348612 GN=Glove_393g47 PE=4 SV=1)

HSP 1 Score: 174.481 bits (441), Expect = 1.054e-47
Identity = 97/209 (46.41%), Postives = 136/209 (65.07%), Query Frame = 1 
Query:  241 ISSN-LIVNHRQKGNGVIDCLRSVRWAFGEIEPDFIMSRTSCALFLSLSYHKVKPSYIFTRIKGIGSLYELRXXXXXXXXXXSTQEIRELSQLCISNNLTLLLAWSNDEAARYLESFKMFENKPPDILTGEKIKSEKLSAAEEEINKYVSFLTSIASINKTDARKLMETFGVLLIPAEFIYCLSQTIEAIMLSDFEQLRSCPGLGALKV 864
            ISSN +IVN  QKGN ++ C+R+V W +GEI PD+++ +T+CALFLSL YH++ P YIF RI+ +   Y LR++L+LVDV    + IREL++LC+ NN T++LAWS +EA RYLE+ K FE+K PD++  EK++ + L+       K    +T I SINKTD   L  +FG              +++ IM + FEQL  CPGLG  KV
Sbjct:  180 ISSNAIIVNSCQKGNPILQCIRNVPWEYGEIVPDYLLGQTTCALFLSLKYHRLHPEYIFNRIEKLAHHYLLRIMLILVDVEIHQEPIRELTKLCVLNNFTIILAWSQEEAGRYLETCKAFEHKSPDMIM-EKVEDDYLA-------KLTHCMTQIKSINKTDVITLASSFG--------------SLKKIMSTSFEQLTLCPGLGHQKV 366
[Close]
BLAST of DNA excision repair protein ERCC-1 vs. TrEMBL
Match: A0A3Q7V4N8 (DNA excision repair protein ERCC-1 isoform X2 OS=Ursus arctos horribilis OX=116960 GN=ERCC1 PE=4 SV=1)

HSP 1 Score: 170.244 bits (430), Expect = 4.741e-47
Identity = 103/271 (38.01%), Postives = 161/271 (59.41%), Query Frame = 1 
Query:   85 GGLFQMYKAGKIDCNIPSSQEQRPITYKTVSIS-PPN-------EFPENAASTSLNTAPII---SSNLIVNHRQKGNGVIDCLRSVRWAFGEIEPDFIMSRTSCALFLSLSYHKVKPSYIFTRIKGIGSLYELRXXXXXXXXXXSTQEIRELSQLCISNNLTLLLAWSNDEAARYLESFKMFENKPPDILTGEKIKSEKLSAAEEEINKYVSFLTSIASINKTDARKLMETFGVLLIPAEFIYCLSQTIEAIMLSDFEQLRSCPGLGALKV 864
             G+  ++K+ +    + +S +  P TY   +IS PP          PE  A  + N AP +   SS++IV+ RQ+GN V+  +R+V W FG++ PD+++ +++CALFLSL YH + P YI  R++ +G  + LRVLLV VDV D  Q ++EL+++CI  + TL+LAWS +EA RYLE++K +E KP D+L       EKL   ++ +++    LT++ S+NKTD++ L+ TFG              ++E +M +  E L  CPGLG  K 
Sbjct:   30 AGVKPLFKSTRSLPTVETSPQAAPQTYAEYAISGPPGGAGAPHPTGPEPLAGETPNQAPKLGAKSSSIIVSPRQRGNPVLKFVRNVPWEFGDVLPDYVLGQSTCALFLSLRYHNLHPDYIHERLQSLGKSFALRVLLVQVDVKDPQQALKELAKMCILADCTLVLAWSPEEAGRYLETYKAYEQKPADLLM------EKLE--QDFVSRVTECLTTVKSVNKTDSQTLLTTFG--------------SLEQLMAASREDLALCPGLGPQKA 278
[Close]
BLAST of DNA excision repair protein ERCC-1 vs. TrEMBL
Match: A0A3Q7UNZ0 (DNA excision repair protein ERCC-1 isoform X1 OS=Ursus arctos horribilis OX=116960 GN=ERCC1 PE=4 SV=1)

HSP 1 Score: 169.859 bits (429), Expect = 7.751e-47
Identity = 103/271 (38.01%), Postives = 161/271 (59.41%), Query Frame = 1 
Query:   85 GGLFQMYKAGKIDCNIPSSQEQRPITYKTVSIS-PPN-------EFPENAASTSLNTAPII---SSNLIVNHRQKGNGVIDCLRSVRWAFGEIEPDFIMSRTSCALFLSLSYHKVKPSYIFTRIKGIGSLYELRXXXXXXXXXXSTQEIRELSQLCISNNLTLLLAWSNDEAARYLESFKMFENKPPDILTGEKIKSEKLSAAEEEINKYVSFLTSIASINKTDARKLMETFGVLLIPAEFIYCLSQTIEAIMLSDFEQLRSCPGLGALKV 864
             G+  ++K+ +    + +S +  P TY   +IS PP          PE  A  + N AP +   SS++IV+ RQ+GN V+  +R+V W FG++ PD+++ +++CALFLSL YH + P YI  R++ +G  + LRVLLV VDV D  Q ++EL+++CI  + TL+LAWS +EA RYLE++K +E KP D+L       EKL   ++ +++    LT++ S+NKTD++ L+ TFG              ++E +M +  E L  CPGLG  K 
Sbjct:   42 AGVKPLFKSTRSLPTVETSPQAAPQTYAEYAISGPPGGAGAPHPTGPEPLAGETPNQAPKLGAKSSSIIVSPRQRGNPVLKFVRNVPWEFGDVLPDYVLGQSTCALFLSLRYHNLHPDYIHERLQSLGKSFALRVLLVQVDVKDPQQALKELAKMCILADCTLVLAWSPEEAGRYLETYKAYEQKPADLLM------EKLE--QDFVSRVTECLTTVKSVNKTDSQTLLTTFG--------------SLEQLMAASREDLALCPGLGPQKA 290
[Close]
BLAST of DNA excision repair protein ERCC-1 vs. TrEMBL
Match: A0A452S1U3 (ERCC excision repair 1, endonuclease non-catalytic subunit OS=Ursus americanus OX=9643 GN=ERCC1 PE=4 SV=1)

HSP 1 Score: 169.088 bits (427), Expect = 1.201e-46
Identity = 103/271 (38.01%), Postives = 160/271 (59.04%), Query Frame = 1 
Query:   85 GGLFQMYKAGKIDCNIPSSQEQRPITYKTVSIS-PPN-------EFPENAASTSLNTAP---IISSNLIVNHRQKGNGVIDCLRSVRWAFGEIEPDFIMSRTSCALFLSLSYHKVKPSYIFTRIKGIGSLYELRXXXXXXXXXXSTQEIRELSQLCISNNLTLLLAWSNDEAARYLESFKMFENKPPDILTGEKIKSEKLSAAEEEINKYVSFLTSIASINKTDARKLMETFGVLLIPAEFIYCLSQTIEAIMLSDFEQLRSCPGLGALKV 864
             G+  ++K+ +    + +S +  P TY   +IS PP          PE  A  + N AP     SS++IV+ RQ+GN V+  +R+V W FG++ PD+++ +++CALFLSL YH + P YI  R++ +G  + LRVLLV VDV D  Q ++EL+++CI  + TL+LAWS +EA RYLE++K +E KP D+L       EKL   ++ +++    LT++ S+NKTD++ L+ TFG              ++E +M +  E L  CPGLG  K 
Sbjct:   30 AGVKPLFKSTRSLPTVETSPQAAPQTYAEYAISGPPGGAGAPHPTGPEPLAGETPNQAPKPGAKSSSIIVSPRQRGNPVLKFVRNVPWEFGDVLPDYVLGQSTCALFLSLRYHNLHPDYIHERLQSLGKSFALRVLLVQVDVKDPQQALKELAKMCILADCTLVLAWSPEEAGRYLETYKAYEQKPADLLM------EKLE--QDFVSRVTECLTTVKSVNKTDSQTLLTTFG--------------SLEQLMAASREDLALCPGLGPQKA 278
[Close]
BLAST of DNA excision repair protein ERCC-1 vs. TrEMBL
Match: A0A384BK83 (DNA excision repair protein ERCC-1 OS=Ursus maritimus OX=29073 GN=ERCC1 PE=4 SV=1)

HSP 1 Score: 169.088 bits (427), Expect = 1.201e-46
Identity = 103/271 (38.01%), Postives = 160/271 (59.04%), Query Frame = 1 
Query:   85 GGLFQMYKAGKIDCNIPSSQEQRPITYKTVSIS-PPN-------EFPENAASTSLNTAP---IISSNLIVNHRQKGNGVIDCLRSVRWAFGEIEPDFIMSRTSCALFLSLSYHKVKPSYIFTRIKGIGSLYELRXXXXXXXXXXSTQEIRELSQLCISNNLTLLLAWSNDEAARYLESFKMFENKPPDILTGEKIKSEKLSAAEEEINKYVSFLTSIASINKTDARKLMETFGVLLIPAEFIYCLSQTIEAIMLSDFEQLRSCPGLGALKV 864
             G+  ++K+ +    + +S +  P TY   +IS PP          PE  A  + N AP     SS++IV+ RQ+GN V+  +R+V W FG++ PD+++ +++CALFLSL YH + P YI  R++ +G  + LRVLLV VDV D  Q ++EL+++CI  + TL+LAWS +EA RYLE++K +E KP D+L       EKL   ++ +++    LT++ S+NKTD++ L+ TFG              ++E +M +  E L  CPGLG  K 
Sbjct:   30 AGVKPLFKSTRSLPTVETSPQAAPQTYAEYAISGPPGGAGAPHPTGPEPLAGETPNQAPKPGAKSSSIIVSPRQRGNPVLKFVRNVPWEFGDVLPDYVLGQSTCALFLSLRYHNLHPDYIHERLQSLGKSFALRVLLVQVDVKDPQQALKELAKMCILADCTLVLAWSPEEAGRYLETYKAYEQKPADLLM------EKLE--QDFVSRVTECLTTVKSVNKTDSQTLLTTFG--------------SLEQLMAASREDLALCPGLGPQKA 278
[Close]
BLAST of DNA excision repair protein ERCC-1 vs. Ensembl Cavefish
Match: ercc1 (ERCC excision repair 1, endonuclease non-catalytic subunit [Source:NCBI gene;Acc:103040846])

HSP 1 Score: 154.451 bits (389), Expect = 9.517e-44
Identity = 87/206 (42.23%), Postives = 130/206 (63.11%), Query Frame = 1 
Query:  247 SNLIVNHRQKGNGVIDCLRSVRWAFGEIEPDFIMSRTSCALFLSLSYHKVKPSYIFTRIKGIGSLYELRXXXXXXXXXXSTQEIRELSQLCISNNLTLLLAWSNDEAARYLESFKMFENKPPDILTGEKIKSEKLSAAEEEINKYVSFLTSIASINKTDARKLMETFGVLLIPAEFIYCLSQTIEAIMLSDFEQLRSCPGLGALKV 864
            +++IV+ RQ+GN ++  +RSV W F E+ PD+++ RT+CALFLSL YH + P+YI  R+K +G  + LRVLLV VDV D    ++EL+++CI  + TL+LAWS +EA RYLE++K +E KP D+L  E+++ + LS       K    LT++ S+NKTDA  L+  F               ++E I+ +  E+L  CPGLG  K 
Sbjct:  159 NSIIVSPRQRGNPILKFVRSVPWEFAEVVPDYVLGRTTCALFLSLRYHNLNPNYIHERLKQLGQTFTLRVLLVQVDVKDPHHALKELARICIMADCTLILAWSPEEAGRYLETYKSYEKKPADLLK-EQVEKDYLS-------KVTDCLTTVKSVNKTDAITLLSAFS--------------SLEGIINASKEELVLCPGLGPQKA 342
[Close]
BLAST of DNA excision repair protein ERCC-1 vs. Ensembl Sea Lamprey
Match: ercc1 (excision repair cross-complementation group 1 [Source:ZFIN;Acc:ZDB-GENE-040426-2606])

HSP 1 Score: 152.525 bits (384), Expect = 7.627e-45
Identity = 89/211 (42.18%), Postives = 130/211 (61.61%), Query Frame = 1 
Query:  244 SSNLIVNHRQKGNGVIDCLRSVRWAFGEIEPDFIMSRTSCALFLSLSYHKVKPSYIFTRIKGIGSLYELRXXXXXXXXXXSTQEIRELSQLCISNNLTLLLAW----SNDEAARYLESFKMFENKPPDILTGEKIKSEKLSAAEEEINKYVSFLTSIASINKTDARKLMETFGVLLIPAEFIYCLSQTIEAIMLSDFEQLRSCPGLGALKV 864
            S++L+VN RQ+GN V+  +R+V W   ++ PD++M +TSCALFLSL YH + P+Y+  R+K +G  ++LRVLLV VDV D    ++EL+++CI    TL+LAW    S +E ARYLE++K +ENKP D+L  E+++ + LS       +    LT+I S+NKTD   L+ TFG              ++  IM +  E L  CPGLG  K 
Sbjct:   58 SNSLLVNPRQRGNPVLKFVRNVPWEIADVVPDYVMGQTSCALFLSLRYHNLNPNYVHERLKQLGQAFDLRVLLVQVDVKDPHHALKELAKMCILAECTLILAWRHADSPEEVARYLETYKAYENKPADLLK-ERVEQDYLS-------RMTDCLTTIKSVNKTDVITLLNTFG--------------SLANIMEASKEDLSLCPGLGPRKA 246
[Close]
BLAST of DNA excision repair protein ERCC-1 vs. Ensembl Yeast
Match: RAD10 (Single-stranded DNA endonuclease (with Rad1p); cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human ERCC1 protein [Source:SGD;Acc:S000004560])

HSP 1 Score: 57.7658 bits (138), Expect = 1.837e-10
Identity = 49/171 (28.65%), Postives = 92/171 (53.80%), Query Frame = 1 
Query:  142 QEQRPITYKTVSIS---------PPNEFPENAASTSLNTA-PIISS----NLIVNHRQKGNGVIDCLRSVRWAF------GEIEPDFIMSRTSCALFLSLSYHKVKPSYIFTRIKGIGSLYELRXXXXXXXXXXSTQEIRELSQLCISNNLTLLLAWSNDEAARYLESFKM 594
            Q+Q P T + ++ +          P E+ ++ A+   N   P  S+     ++VN  QK N +++ L+S  W +        I  D+++ R    LFL+L+YHK+   YI  R++ + S  E  +L+ +VD  +S   + ++++LC+ N  TLLLA++ ++AA+Y+E   +
Sbjct:   42 QQQEPQTSRRINSNQVINAFNQQKPEEWTDSKATDDYNRKRPFRSTRPGKTVLVNTTQKENPLLNHLKSTNWRYVSSTGINMIYYDYLV-RGRSVLFLTLTYHKLYVDYISRRMQPL-SRNENNILIFIVDDNNSEDTLNDITKLCMFNGFTLLLAFNFEQAAKYIEYLNL 210
[Close]
BLAST of DNA excision repair protein ERCC-1 vs. Ensembl Medaka
Match: ercc1 (ERCC excision repair 1, endonuclease non-catalytic subunit [Source:NCBI gene;Acc:101168825])

HSP 1 Score: 153.295 bits (386), Expect = 1.762e-43
Identity = 87/206 (42.23%), Postives = 132/206 (64.08%), Query Frame = 1 
Query:  247 SNLIVNHRQKGNGVIDCLRSVRWAFGEIEPDFIMSRTSCALFLSLSYHKVKPSYIFTRIKGIGSLYELRXXXXXXXXXXSTQEIRELSQLCISNNLTLLLAWSNDEAARYLESFKMFENKPPDILTGEKIKSEKLSAAEEEINKYVSFLTSIASINKTDARKLMETFGVLLIPAEFIYCLSQTIEAIMLSDFEQLRSCPGLGALKV 864
            S++IV+ RQ+GN ++  +RSV W FG++ PD+++ +T+CALFLSL YH + P+YI  R+K +G+ + LRVLLV VDV D    ++EL+++ +  + TL+LAWS +EA RYLE++K +E KP D+L  E+++ + LS       K    LT++ SINKTDA  L+ TF               ++E I+ +  E+L  CPGLG  K 
Sbjct:  139 SSIIVSPRQRGNPILKFVRSVPWEFGDVVPDYVLGQTTCALFLSLRYHNLNPNYIHERLKQLGNSFTLRVLLVQVDVKDPHHALKELARIGVMADCTLILAWSPEEAGRYLETYKSYEKKPADLLK-EQVEKDYLS-------KVTDCLTTVKSINKTDAITLLSTFS--------------SVEGIISASKEELVLCPGLGPQKA 322
[Close]
BLAST of DNA excision repair protein ERCC-1 vs. Planmine SMEST
Match: SMESG000048855.1 (SMESG000048855.1)

HSP 1 Score: 515.383 bits (1326), Expect = 0.000e+0
Identity = 266/281 (94.66%), Postives = 266/281 (94.66%), Query Frame = 1 
Query:   22 MSTEDHFDHIASHIESPIIEKGGLFQMYKAGKIDCNIPSSQEQRPITYKTVSISPPNEFPENAASTSLNTAPIISSNLIVNHRQKGNGVIDCLRSVRWAFGEIEPDFIMSRTSCALFLSLSYHKVKPSYIFTRIKGIGSLYELRXXXXXXXXXXSTQEIRELSQLCISNNLTLLLAWSNDEAARYLESFKMFENKPPDILTGEKIKSEKLSAAEEEINKYVSFLTSIASINKTDARKLMETFGVLLIPAEFIYCLSQTIEAIMLSDFEQLRSCPGLGALKV 864
            MSTEDHFDHIASHIESPIIEKGGLFQMYKAGKIDCNIPSSQEQRPITYKTVSISPPNEFPENAASTSLNTAPIISSNLIVNHRQKGNGVIDCLRSVRWAFGEIEPDFIMSRTSCALFLSLSYHKVKPSYIFTRIKGIGSLYELRVLLVLVDVVDSTQEIRELSQLCISNNLTLLLAWSNDEAARYLESFKMFENKPPDILTGEKIKSEKLSAAEEEINKYVSFLTSIASINKTDARKLMETFG              TIEAIMLSDFEQLRSCPGLGALK 
Sbjct:    1 MSTEDHFDHIASHIESPIIEKGGLFQMYKAGKIDCNIPSSQEQRPITYKTVSISPPNEFPENAASTSLNTAPIISSNLIVNHRQKGNGVIDCLRSVRWAFGEIEPDFIMSRTSCALFLSLSYHKVKPSYIFTRIKGIGSLYELRVLLVLVDVVDSTQEIRELSQLCISNNLTLLLAWSNDEAARYLESFKMFENKPPDILTGEKIKSEKLSAAEEEINKYVSFLTSIASINKTDARKLMETFG--------------TIEAIMLSDFEQLRSCPGLGALKA 267
The following BLAST results are available for this feature:
BLAST of DNA excision repair protein ERCC-1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ERCC11.106e-4538.52ERCC excision repair 1, endonuclease non-catalytic... [more]
ERCC11.106e-4538.52ERCC excision repair 1, endonuclease non-catalytic... [more]
ERCC11.239e-4539.06ERCC excision repair 1, endonuclease non-catalytic... [more]
ERCC17.988e-4341.10ERCC excision repair 1, endonuclease non-catalytic... [more]
ERCC14.471e-4240.82ERCC excision repair 1, endonuclease non-catalytic... [more]
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BLAST of DNA excision repair protein ERCC-1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 2
Match NameE-valueIdentityDescription
ercc-14.603e-2432.86ERCC (DNA excision repair protein) homolog [Sourc... [more]
ercc-14.603e-2432.86ERCC (DNA excision repair protein) homolog [Sourc... [more]
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BLAST of DNA excision repair protein ERCC-1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Ercc13.698e-3439.05gene:FBgn0028434 transcript:FBtr0087448[more]
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BLAST of DNA excision repair protein ERCC-1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ercc11.480e-4340.78excision repair cross-complementation group 1 [Sou... [more]
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BLAST of DNA excision repair protein ERCC-1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 3
Match NameE-valueIdentityDescription
ercc19.522e-4240.91excision repair cross-complementation group 1 [Sou... [more]
ercc11.987e-4140.72excision repair cross-complementation group 1 [Sou... [more]
ercc12.998e-4140.72excision repair cross-complementation group 1 [Sou... [more]
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BLAST of DNA excision repair protein ERCC-1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 3
Match NameE-valueIdentityDescription
Ercc15.725e-4643.00excision repair cross-complementing rodent repair ... [more]
Ercc18.046e-3648.91excision repair cross-complementing rodent repair ... [more]
Ercc16.398e-1845.71excision repair cross-complementing rodent repair ... [more]
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BLAST of DNA excision repair protein ERCC-1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q1LZ75|ERCC1_BOVIN2.243e-4736.36DNA excision repair protein ERCC-1 OS=Bos taurus O... [more]
sp|P07903|ERCC1_MOUSE4.005e-4543.00DNA excision repair protein ERCC-1 OS=Mus musculus... [more]
sp|P07992|ERCC1_HUMAN5.313e-4538.52DNA excision repair protein ERCC-1 OS=Homo sapiens... [more]
sp|Q06182|SWI10_SCHPO7.240e-3939.71Mating-type switching protein swi10 OS=Schizosacch... [more]
sp|Q9MA98|ERCC1_ARATH6.303e-3638.25DNA excision repair protein ERCC-1 OS=Arabidopsis ... [more]
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BLAST of DNA excision repair protein ERCC-1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A397H2E61.054e-4746.41UPF0029 domain-containing protein OS=Diversispora ... [more]
A0A3Q7V4N84.741e-4738.01DNA excision repair protein ERCC-1 isoform X2 OS=U... [more]
A0A3Q7UNZ07.751e-4738.01DNA excision repair protein ERCC-1 isoform X1 OS=U... [more]
A0A452S1U31.201e-4638.01ERCC excision repair 1, endonuclease non-catalytic... [more]
A0A384BK831.201e-4638.01DNA excision repair protein ERCC-1 OS=Ursus mariti... [more]
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BLAST of DNA excision repair protein ERCC-1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ercc19.517e-4442.23ERCC excision repair 1, endonuclease non-catalytic... [more]
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BLAST of DNA excision repair protein ERCC-1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ercc17.627e-4542.18excision repair cross-complementation group 1 [Sou... [more]
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BLAST of DNA excision repair protein ERCC-1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1
Match NameE-valueIdentityDescription
RAD101.837e-1028.65Single-stranded DNA endonuclease (with Rad1p); cle... [more]
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BLAST of DNA excision repair protein ERCC-1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DNA excision repair protein ERCC-1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ercc11.762e-4342.23ERCC excision repair 1, endonuclease non-catalytic... [more]
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BLAST of DNA excision repair protein ERCC-1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000048855.10.000e+094.66SMESG000048855.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30028315 ID=SMED30028315|Name=DNA excision repair protein ERCC-1|organism=Schmidtea mediterranea sexual|type=transcript|length=982bp
TTATAATTATCGAATTTCAAAATGAGCACAGAGGACCATTTTGATCATAT
AGCATCTCACATTGAATCGCCAATTATAGAAAAGGGTGGATTATTTCAAA
TGTACAAGGCGGGTAAAATCGACTGCAATATACCATCTTCTCAAGAACAA
AGACCAATAACATATAAAACTGTTAGCATTTCTCCCCCAAATGAATTTCC
GGAAAATGCTGCTTCAACATCCTTAAACACAGCCCCAATTATTTCATCAA
ACTTGATTGTGAATCACCGACAGAAAGGTAATGGTGTAATTGATTGTCTG
AGGAGTGTCAGATGGGCATTTGGTGAAATCGAACCGGATTTCATTATGAG
TAGAACATCATGCGCTTTATTTTTAAGTTTAAGTTATCACAAAGTGAAGC
CGAGCTATATCTTTACTCGAATCAAAGGAATTGGATCACTTTATGAATTG
CGGGTTTTACTAGTTCTCGTTGACGTAGTAGATTCAACTCAGGAAATACG
TGAGCTCTCTCAACTATGTATTTCCAATAATCTTACATTGCTGTTAGCTT
GGAGCAATGATGAAGCGGCCCGTTACTTAGAATCATTCAAAATGTTTGAA
AATAAACCTCCGGATATTTTAACTGGTGAAAAAATAAAATCGGAAAAGTT
ATCGGCAGCCGAGGAGGAAATAAACAAATATGTTTCTTTTCTAACTTCAA
TTGCATCAATTAATAAAACTGACGCTAGAAAATTAATGGAAACTTTTGGA
GTACTTTTAATTCCTGCCGAATTTATTTACTGTCTTTCACAGACAATTGA
AGCCATTATGCTATCAGACTTTGAGCAGCTTAGGTCGTGTCCAGGTTTGG
GAGCTCTTAAGGTTTTGTTGGGATTATTTTTCACATTCATTATTGAATTT
AGGCCAGAAATTTGTATGATTTATTTAGGAAACCTATTAGGAAACACGAA
AGGCAATAGTTATTGATAAAATTGTTATTGGT
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protein sequence of SMED30028315-orf-1

>SMED30028315-orf-1 ID=SMED30028315-orf-1|Name=SMED30028315-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=315bp
MSTEDHFDHIASHIESPIIEKGGLFQMYKAGKIDCNIPSSQEQRPITYKT
VSISPPNEFPENAASTSLNTAPIISSNLIVNHRQKGNGVIDCLRSVRWAF
GEIEPDFIMSRTSCALFLSLSYHKVKPSYIFTRIKGIGSLYELRVLLVLV
DVVDSTQEIRELSQLCISNNLTLLLAWSNDEAARYLESFKMFENKPPDIL
TGEKIKSEKLSAAEEEINKYVSFLTSIASINKTDARKLMETFGVLLIPAE
FIYCLSQTIEAIMLSDFEQLRSCPGLGALKVLLGLFFTFIIEFRPEICMI
YLGNLLGNTKGNSY*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: biological process
TermDefinition
GO:0060261positive regulation of transcription initiation from RNA polymerase II promoter
GO:0006281DNA repair
GO:0000710meiotic mismatch repair
GO:0000720pyrimidine dimer repair by nucleotide-excision repair
GO:0001302replicative cell aging
GO:0032205negative regulation of telomere maintenance
GO:0035166post-embryonic hemopoiesis
GO:0035264multicellular organism growth
GO:0036297interstrand cross-link repair
GO:0045190isotype switching
GO:0048468cell development
GO:0048477oogenesis
GO:0051276chromosome organization
GO:0061819telomeric DNA-containing double minutes formation
GO:0070914UV-damage excision repair
GO:0090656t-circle formation
GO:1904431positive regulation of t-circle formation
GO:1905765negative regulation of protection from non-homologous end joining at telomere
GO:0006289nucleotide-excision repair
GO:0006295nucleotide-excision repair, DNA incision, 3'-to lesion
GO:0006296nucleotide-excision repair, DNA incision, 5'-to lesion
GO:0006302double-strand break repair
GO:0006303double-strand break repair via nonhomologous end joining
GO:0006310DNA recombination
GO:0006312mitotic recombination
GO:0006949syncytium formation
GO:0006974cellular response to DNA damage stimulus
GO:0006979response to oxidative stress
GO:0007281germ cell development
GO:0007283spermatogenesis
GO:0008283cell proliferation
GO:0008584male gonad development
GO:0009650UV protection
GO:0010165response to X-ray
GO:0010259multicellular organism aging
Vocabulary: molecular function
TermDefinition
GO:1990841promoter-specific chromatin binding
GO:0003684damaged DNA binding
GO:0004519endonuclease activity
GO:0000014single-stranded DNA endodeoxyribonuclease activity
GO:0001094TFIID-class transcription factor binding
GO:19905993' overhang single-stranded DNA endodeoxyribonuclease activity
GO:0003677DNA binding
GO:0003697single-stranded DNA binding
GO:0004518nuclease activity
GO:0008022protein C-terminus binding
GO:0016787hydrolase activity
GO:0019904protein domain specific binding
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000014zeta neoblast
PLANA:0002075pigment cell
PLANA:0002109X1 cell
PLANA:0002111X2 cell
Vocabulary: INTERPRO
TermDefinition
IPR004579ERCC1/RAD10/SWI10
IPR011335Restrct_endonuc-II-like
Vocabulary: cellular component
TermDefinition
GO:0005634nucleus
GO:0000109nucleotide-excision repair complex
GO:0000110nucleotide-excision repair factor 1 complex
GO:0000784nuclear chromosome, telomeric region
GO:0070522ERCC4-ERCC1 complex
GO:0005654nucleoplasm
GO:0005669transcription factor TFIID complex
GO:0005829cytosol
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:1.10.150.20coord: 192..246
e-value: 1.4E-6
score: 29.7
NoneNo IPR availableGENE3DG3DSA:3.40.50.10130coord: 76..191
e-value: 9.1E-43
score: 146.6
NoneNo IPR availablePRODOMPD013585coord: 78..200
e-value: 2.0E-26
score: 297.0
IPR004579ERCC1/RAD10/SWI10 familyPFAMPF03834Rad10coord: 78..190
e-value: 3.2E-42
score: 143.2
IPR004579ERCC1/RAD10/SWI10 familyTIGRFAMTIGR00597TIGR00597coord: 78..187
e-value: 1.8E-38
score: 128.8
IPR004579ERCC1/RAD10/SWI10 familyPANTHERPTHR12749EXCISION REPAIR CROSS-COMPLEMENTING 1 ERCC1coord: 44..281
IPR011335Restriction endonuclease type II-likeSUPERFAMILYSSF52980Restriction endonuclease-likecoord: 77..202