DDE_3 domain-containing protein

Overview
NameDDE_3 domain-containing protein
Smed IDSMED30028299
Length (bp)1274
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of DDE_3 domain-containing protein (SMED30028299) t-SNE clustered cells

Violin plots show distribution of expression levels for DDE_3 domain-containing protein (SMED30028299) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of DDE_3 domain-containing protein (SMED30028299) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for DDE_3 domain-containing protein (SMED30028299) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30028299

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 11

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30028299 SmedASXL_015830SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
protonephridiaSMED30028299 dd_Smed_v4_6265_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30028299 dd_Smed_v4_6265_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30028299 Contig38011newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30028299 Contig38011uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
neoblastSMED30028299 dd_Smed_v4_6389_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30028299 dd_Smed_v4_6389_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
ovarySMED30028299 Contig2115GPL15192PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite colorimetric in situ hybridization evidence
ovarySMED30028299 PL05008A1C08ncbi_smed_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite colorimetric in situ hybridization evidence
reproductive organSMED30028299 Contig27445newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30028299 Contig27445uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of DDE_3 domain-containing protein vs. TrEMBL
Match: W4XP44 (DDE_3 domain-containing protein OS=Strongylocentrotus purpuratus OX=7668 PE=4 SV=1)

HSP 1 Score: 123.25 bits (308), Expect = 8.816e-28
Identity = 93/359 (25.91%), Postives = 168/359 (46.80%), Query Frame = 1 
Query:  271 ISQTDKMKLVEAME-KGEDVFKLARQMGIGRNTAKSIIYVFKKTGKIFQGKR--LKNPNISEELTQKLLNFVDKNYWLTLEEIRKHL---VDQNYEISVYCVSRCLRRNIVAFNKFRSHIAERNSEKVLELRKSYVRRLLEEEWNDNKMIYIDECYFNTWGSKTLSRTKMGVLPAALV--------PNICFVIAIGVKGIIHHKLLIGSLDKESYHVFINELTVKLN-----GTGYILIHDNVKSY-----RENFSTDNQTVNLPPYSSFLSPLDEVFTKIKGNVRQSLSHIKEFTSMNIDNRFVFLNDAINNELKKEDY--------------SNLKIYFKRIRTFFPKAILKEEISG 1233
            IS  D+ +LVEA E  G+D  +LA  +GI R+TA+SI+  +  +G+I +  R   +N  I EE+   L + +D N  LTL +++  L   + Q  E+S   ++R L   ++           RN+ +VL+ R+ Y    LE     N  ++IDEC +N W  +T  R   GV PA  V         N+ F ++  V G++HH++   ++ + S+  F+     + N         +LI+DN + +      E+ ++  +T  LPPYS FL+  +   +  K  V+++L+         + +R       +N +  + D               +    ++   +T+ P+ + ++EI G
Sbjct:   10 ISNVDRRRLVEAFEGNGQDYLELADNLGIKRSTARSIVATYLDSGRIEKLPRGGARNSKIDEEMKNYLQDIIDTNPLLTLRQMKDKLREDLPQKPEVSTTTIARALDAMLMTLKLAEDVPDARNAPRVLQQREEYAEWFLEHGVLAN-CVFIDECGYNIWTRRTQGRAPRGV-PARRVVHGQRGRNCNVSFAVSAAV-GLVHHEMAFETVTRASFERFLAATVAECNEMFPDDAPVVLIYDNARPHVRAELPEDVNSLIETKRLPPYSPFLNMTEMAHSSFKSAVKRTLA--LPAWQRRVGDRVAAGRAGVNLQQWRCDALREVAVQCIDTVTPAKCTQWYNHSQTYMPRCLARQEIDG 363
[Close]
BLAST of DDE_3 domain-containing protein vs. TrEMBL
Match: W4Z6F7 (DDE_3 domain-containing protein OS=Strongylocentrotus purpuratus OX=7668 PE=4 SV=1)

HSP 1 Score: 121.324 bits (303), Expect = 5.150e-27
Identity = 93/359 (25.91%), Postives = 168/359 (46.80%), Query Frame = 1 
Query:  271 ISQTDKMKLVEAME-KGEDVFKLARQMGIGRNTAKSIIYVFKKTGKIFQGKR--LKNPNISEELTQKLLNFVDKNYWLTLEEIRKHL---VDQNYEISVYCVSRCLRRNIVAFNKFRSHIAERNSEKVLELRKSYVRRLLEEEWNDNKMIYIDECYFNTWGSKTLSRTKMGVLPAALV--------PNICFVIAIGVKGIIHHKLLIGSLDKESYHVFINELTVKLN-----GTGYILIHDNVKSY-----RENFSTDNQTVNLPPYSSFLSPLDEVFTKIKGNVRQSLSHIKEFTSMNIDNRFVFLNDAINNELKKEDY--------------SNLKIYFKRIRTFFPKAILKEEISG 1233
            IS  D+ +LVEA E  G+D  +LA  +GI R+TA+SI+  +  +G+I +  R   +N  I EE+   L + +D N  LTL +++  L   + Q  E+S   ++R L   ++           RN+ +VL+ R  Y    LE     N  ++IDEC +N W  +T  R   GV PA  V         N+ F ++  V G++HH++   ++ + S+  F+     + N         +LI+DN + +      E+ ++  +T  LPPYS FL+  +   +  K  V+++L+         + +R     + +N +  + D               +    ++   +T+ P+ + ++EI G
Sbjct:   10 ISNVDRRRLVEAFEGNGQDYLELADNLGIKRSTARSIVATYLDSGRIEKLPRGGARNSKIDEEMKNYLQDIIDTNPLLTLRQMKDKLREDLPQKPEVSTTTIARALDVMLMTLKLAEDVPDARNAPRVLQQRGEYAEWFLEHGVLAN-CVFIDECGYNIWTRRTQGRAPRGV-PARRVVHGQRGRNCNVSFAVSAAV-GLVHHEMAFETVTRASFERFLAAKVAECNEMFPDDAPVVLIYDNARPHVRAELPEDVNSLIETKRLPPYSPFLNMTEMAHSSFKSAVKRTLA--LPAWQRRVGDRVAAGREGVNLQQWRCDTLREVAVQCIDTVTPAKCTQWYNHSQTYMPRCLERQEIDG 363
[Close]
BLAST of DDE_3 domain-containing protein vs. TrEMBL
Match: A0A267DQJ8 (DDE_3 domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig002000g2 PE=4 SV=1)

HSP 1 Score: 119.783 bits (299), Expect = 1.651e-26
Identity = 76/293 (25.94%), Postives = 153/293 (52.22%), Query Frame = 1 
Query:  250 ERPAKK---SISQTDKMKLVEAMEKGEDVFKLARQMGIGRNTAKSIIYVFKKTGKIFQGKRLKNP--NISEELTQKLLNFVDKNYWLTLEEIRKHLVDQ--NYE-ISVYCVSRCLRRNIVAFNKFRSHIAERNSEKVLELRKSYVRRLLEEEWNDNKMIYIDECYFNTWGSKTLSRTKMGVLPAALVPN-------ICFVIAIGVKGIIHHKLLIGSLDKESYHVFINELTVKLNGTGYILIHDNVKSYRE--NFSTDNQTVNLPPYSSFLSPLDEVFTKIKGNVRQSLSHIK 1077
            E PA +   ++SQ D+ +LV A    ED   LA Q+ I R +A+++I  F++TG++   KR  N    +S+E+ + L+ FV      TL+++R+HL+    +++  ++  +SR L   ++     R+     N++++   R+ + + L+ E      +++ DE   N W ++T  R   GV    +V N       +C  ++  ++G+++ K++ G + +E +  F  EL   L    + L+HDN +++ +      + +   LPPYS FL+  +     +K ++++SLS ++
Sbjct:    2 EAPAAQRRVAVSQQDRRRLVNAFVLNEDWLNLAEQLNIPRQSARNVIVKFERTGRVEAEKRGGNKPKALSDEMIEALVGFVADTPAATLQDMRRHLMQAFPDWQACTIQTISRALDGQLITVKLLRTIPNAWNADRIKHERREFAKWLMTEGMG-KLLVFCDEMGCNVWTARTQGRAAEGVRAVRIVANQRGRNVTMCLAVS-PMRGLVYWKIVRGGMTRELFAEFFAELAA-LVDEPFCLLHDNARAHWDPPAMGEEQEYRPLPPYSPFLNMTERAIAAVKASLKRSLSRVE 291
[Close]
BLAST of DDE_3 domain-containing protein vs. TrEMBL
Match: W4Z474 (DDE_3 domain-containing protein OS=Strongylocentrotus purpuratus OX=7668 PE=4 SV=1)

HSP 1 Score: 112.464 bits (280), Expect = 1.136e-23
Identity = 89/360 (24.72%), Postives = 159/360 (44.17%), Query Frame = 1 
Query:  271 ISQTDKMKLVEAMEKGEDVFKLARQMGIGRNTAKSIIYVFKKTGKIFQGKR--LKNPNISEELTQKLLNFVDKNYWLTLEEIRK---HLVDQNYEISVYCVSRCLRRNIVAFNKFRSHIAERNSEKVLELRKSYVRRLLEEEWNDNKMIYIDECYFNTWGSKTLSRTKMGVLPAALV--------PNICFVIAIGVKGIIHHKL----LIGSLDKESYHVFINELTVKLN-----GTGYILIHDNVKSYRENFSTDN-----QTVNLPPYSSFLSPLDEVFTKIKGNVRQSLS-----------HIKEFTSMNIDN-RFVFLNDAINNELKKEDYSNLKIYFKRIRTFFPKAILKEEISG 1233
            IS TD+ +L EA +  +D  +LA  +GI R+TA+SI+  +  TG++ +  R   +N  +  ++   L N +D N  LTL ++++   H +     +S   V+R L   ++           RN+ +V+E R  Y    LE     N  ++IDE  +N W  +T  R   GV PA  V         N+ F +++ + G++HH++    +   + +E +  F+ E   + N          LI+DN + +      D      QT  LPPYS FL+  +   +  K  V+++L+              +   +N+   R   L DA    +          ++   +T+ P+ +  + I G
Sbjct:   40 ISNTDRARLAEAFDNQQDYLELADTLGINRSTARSIVATYVDTGRVQKLPRGGARNVKMDVDMRNHLENIIDANPLLTLRQMKESLAHNLPDKPAVSTSSVARALDGMMLTLKMAEDVPDARNAPRVVEQRLEYAEWFLEHGVVGN-CVFIDETGYNVWTRRTRGRAPRGV-PARRVVHGQRGRNCNVTFAVSVAI-GLVHHRIAFETVTRDVTRERFEDFLAETVAECNQMFPADEPIYLIYDNARPHVRAQLPDGANPIIQTKRLPPYSPFLNMTEMAHSAFKAAVKRTLAQPAWQLRVGDRQAAQDAGINLQQWRCDILRDAALQNIDAITPGKCTQWYNHSQTYLPRCLASQHIDG 396
[Close]
BLAST of DDE_3 domain-containing protein vs. TrEMBL
Match: A0A0C2JI12 (DDE_3 domain-containing protein OS=Thelohanellus kitauei OX=669202 GN=RF11_01522 PE=4 SV=1)

HSP 1 Score: 108.612 bits (270), Expect = 1.222e-22
Identity = 83/300 (27.67%), Postives = 154/300 (51.33%), Query Frame = 1 
Query:  259 AKKSISQTDKMKLVEAMEKGEDVFKLARQMGIGRNTAKSIIYVFKKTGKIFQGKR--LKNPNISEELTQKLLNFVDKNYWLTLEEIRKHLVDQNYEISV--YCVSRCLRRNIVAFNKFRSHIAERNSEKVLELRKSYVRRLLE--EEWNDNKMIYIDECYFNTWGSKTLSRTKMGVLPAALVP-----NICFVIAIGVKGIIHHKLLIGSLDKESYHVFINELTVK---LNGTGYILIHDNVKSYR-----ENFSTDNQT-VNLPPYSSFLSPLDEVFTKIKGNVRQSLSHIKEFTSMNI 1098
            A   +S  D+ ++V+A +KG+   +++  +G+ R+T  S+I +F ++G+I   KR  ++   ++E+  +++ ++VD N  + L  I    V +  +ISV    + R L+R   ++ +       RN+E  L +R+ Y  R LE  + + +++ I+IDEC  N     +  R+K+G     +VP     NI    A+ V G++ HK    + +++ +  +I+EL  K   LN     ++ DNV  +      +  S    T + LPPYS F++P++ +F+K K  +R   S   E    NI
Sbjct:    2 ANNKLSDLDRKRIVDAYQKGQKASEISLVLGVARSTINSVIKIFHQSGRIDSNKRGYIQPEKLNEDQKERIKSWVDDNAGIPLRTIVTK-VQEEMDISVGKSTIDRILQRFHYSWKRVSIIPERRNNESTLNIRERYATRYLELLQSFREHQFIFIDECGVNISMRTSHGRSKIGSPAIHVVPNLRSRNISICAAMNVNGMLLHKWHDRAFNRDLFGNYIDELLEKIRALNIEHATIVMDNVSFHHCEEISQQISEAGHTLLFLPPYSPFMNPIENMFSKWKREIRYRRSENSEELYQNI 300
[Close]
BLAST of DDE_3 domain-containing protein vs. Planmine SMEST
Match: SMESG000041908.1 (SMESG000041908.1)

HSP 1 Score: 305.834 bits (782), Expect = 7.090e-93
Identity = 155/322 (48.14%), Postives = 218/322 (67.70%), Query Frame = 1 
Query:  262 KKSISQTDKMKLVEAMEKGEDVFKLARQMGIGRNTAKSIIYVFKKTGKIFQGKRLKN-PNISEELTQKLLNFVDKNYWLTLEEIRKHLVDQNYEISVYCVSRCLRRNIVAFNKFRSHIAERNSEKVLELRKSYVRRLLEEEWNDNKMIYIDECYFNTWGSKTLSRTKMGVLPAALVP-----NICFVIAIGVKGIIHHKLLIGSLDKESYHVFINELTVKLNGTGYILIHDNVKSYRENFSTDNQTVNLPPYSSFLSPLDEVFTKIKGNVRQSLSHIKEFTSMNIDNRFVFLNDAINNELKKEDYSNLKIYFKRIRTFFPKA 1209
            +K IS+ DK++++ AME GED F   + +GI RN+ K+IIY ++K GK+ QGK   +   ISEE   KLL+FV+KN + +LEE++  L+ +N  +S   +SR L  +++   + R  +AERNS++VL+LR  Y  R LEE W D+++IYIDEC FNTW S+ L R K G    A +P     N+   +AIGV G +HHKL++GS  K++Y +FINE+TVKLNGTG+ LIHDN   +    S +N   +LPPYS FL+P++ VF+KIK N+R+S+S     +SMN  +RF FL DA++NEL KEDY +L  YF  IR FFPK+
Sbjct:  505 RKKISEIDKIRIINAMENGEDYFHFGKLLGIARNSIKTIIYKYRKFGKLSQGKIGGSISKISEENGNKLLDFVEKNVYASLEEMKNFLISENLSVSTNTISRFLENHLITTKQIRPSVAERNSDRVLDLRMEYTTRYLEEAWMDSQLIYIDECGFNTWTSQKLGRNKKGERLYATLPTNRGRNMSLALAIGVNGPVHHKLVVGSYKKDTYQIFINEITVKLNGTGFRLIHDNASIHGGAISLNNVIEHLPPYSPFLNPIESVFSKIKFNIRKSISRAGGLSSMNFQSRFDFLKDALDNELVKEDYRDLTNYFWHIRRFFPKS 826
[Close]
BLAST of DDE_3 domain-containing protein vs. Planmine SMEST
Match: SMESG000005064.1 (SMESG000005064.1)

HSP 1 Score: 298.516 bits (763), Expect = 1.116e-90
Identity = 162/360 (45.00%), Postives = 227/360 (63.06%), Query Frame = 1 
Query:  202 SDEISDAS--SDKTVPFRE----------RPAKKS---------ISQTDKMKLVEAMEKGEDVFKLARQMGIGRNTAKSIIYVFKKTGKIFQGKRLKNPN-ISEELTQKLLNFVDKNYWLTLEEIRKHLVDQNYEISVYCVSRCLRRNIVAFNKFRSHIAERNSEKVLELRKSYVRRLLEEEWNDNKMIYIDECYFNTWGSKTLSRTKMGVLPAALVP-----NICFVIAIGVKGIIHHKLLIGSLDKESYHVFINELTVKLNGTGYILIHDNVKSYRENFSTDNQTVNLPPYSSFLSPLDEVFTKIKGNVRQSLSHIKEFTSMNIDNRFVFLNDAINNELKKEDYSNLKIYFKRIRTFF 1200
            +DEIS  S  S + +PFR+          RP+            IS+ DK++++ AME GED F   + +GI RN+ K+IIY ++K  K+ QGK   N + ISEE   KLL+FV+KN + +L E++  L+ +N  +S   +SR L  +++   + R  +AERNS++VL+ R  Y  R LEE W D+++IYIDEC FNTW S+ L R K G    A +P     N+   +A GV G +HHKL++GS  K++Y +FINE+TVKLNGTGY LIHDN   +    S +N   +LPPYS FL+ ++ VF+KIK N+R+S+S    F+SMN  +RF FL DA++NEL KEDYS+L  YF  IR FF
Sbjct:   86 NDEISSLSDISAEIIPFRDKEITLLSKVARPSTTPETLTKKHTLISEIDKIRIINAMENGEDYFYFGKLLGISRNSIKTIIYKYRKFEKLSQGKIGGNKSKISEEHGNKLLDFVEKNVYASLVEMKNFLISENLSVSTNTISRFLGNHLITTKQIRPSVAERNSDRVLDFRMKYTTRYLEEAWMDSQLIYIDECGFNTWTSQKLGRNKKGERLYATLPTNRGRNMSLALAFGVNGPVHHKLVVGSYKKDTYQIFINEITVKLNGTGYRLIHDNASIHGGAISLNNAIEHLPPYSPFLNLIESVFSKIKFNIRKSISRAGGFSSMNFQSRFDFLKDALDNELVKEDYSDLTNYFGHIRRFF 445
[Close]
BLAST of DDE_3 domain-containing protein vs. Planmine SMEST
Match: SMESG000005064.1 (SMESG000005064.1)

HSP 1 Score: 276.944 bits (707), Expect = 5.091e-83
Identity = 150/341 (43.99%), Postives = 214/341 (62.76%), Query Frame = 1 
Query:  202 SDEISDAS--SDKTVPFRE----------RPAKKS---------ISQTDKMKLVEAMEKGEDVFKLARQMGIGRNTAKSIIYVFKKTGKIFQGKRLKNPN-ISEELTQKLLNFVDKNYWLTLEEIRKHLVDQNYEISVYCVSRCLRRNIVAFNKFRSHIAERNSEKVLELRKSYVRRLLEEEWNDNKMIYIDECYFNTWGSKTLSRTKMGVLPAALVP-----NICFVIAIGVKGIIHHKLLIGSLDKESYHVFINELTVKLNGTGYILIHDNVKSYRENFSTDNQTVNLPPYSSFLSPLDEVFTKIKGNVRQSLSHIKEFTSMNIDNRFVFLNDAINNEL 1143
            +DEIS  S  S + +PFR+          RP+            IS+ DK++++ AME GED F   + +GI RN+ K+IIY ++K  K+ QGK   N + ISEE   KLL+FV+KN + +L E++  L+ +N  +S   +SR L  +++   + R  +AERNS++VL+ R  Y  R LEE W D+++IYIDEC FNTW S+ L R K G    A +P     N+   +A GV G +HHKL++GS  K++Y +FINE+TVKLNGTGY LIHDN   +    S +N   +LPPYS FL+ ++ VF+KIK N+R+S+S    F+SMN  +RF FL DA++NEL
Sbjct:   69 NDEISSLSDISAEIIPFRDKEITLLSKVARPSTTPETLTKKHTLISEIDKIRIINAMENGEDYFYFGKLLGISRNSIKTIIYKYRKFEKLSQGKIGGNKSKISEEHGNKLLDFVEKNVYASLVEMKNFLISENLSVSTNTISRFLGNHLITTKQIRPSVAERNSDRVLDFRMKYTTRYLEEAWMDSQLIYIDECGFNTWTSQKLGRNKKGERLYATLPTNRGRNMSLALAFGVNGPVHHKLVVGSYKKDTYQIFINEITVKLNGTGYRLIHDNASIHGGAISLNNAIEHLPPYSPFLNLIESVFSKIKFNIRKSISRAGGFSSMNFQSRFDFLKDALDNEL 409
[Close]
BLAST of DDE_3 domain-containing protein vs. Planmine SMEST
Match: SMESG000069530.1 (SMESG000069530.1)

HSP 1 Score: 240.35 bits (612), Expect = 4.521e-77
Identity = 121/249 (48.59%), Postives = 163/249 (65.46%), Query Frame = 1 
Query:  508 IRKHLVDQNYEISVYCVSRCLRRNIVAFNKFRSHIAERNSEKVLELRKSYVRRLLEEEWNDNKMIYIDECYFNTWGSKTLSRTKMG-----VLPAALVPNICFVIAIGVKGIIHHKLLIGSLDKESYHVFINELTVKLNGTGYILIHDNVKSYRENFSTDNQTVNLPPYSSFLSPLDEVFTKIKGNVRQSLSHIKEFTSMNIDNRFVFLNDAINNELKKEDYSNLKIYFKRIRTFFPKAILKEEISGFY 1239
            ++  L+ +N  +S   +SR L  +++   + R  +AERNS++VL+LR  Y  R LEE W D+++IYIDEC FNTW S+ L R K G      LP     N+   +AIGV G +HHKL++GS  K++Y VFINE+TVKLNGTG+ LIHDN   +    S +N   +LPPYS FL+P++ VF+KIK N+R+S+S     +SMN  +RF FL DA++NEL KEDY +L  YF  IR FFPK I K  I G Y
Sbjct:    1 MKNFLISENLSVSTNTISRFLGNHLITTKQIRPSVAERNSDRVLDLRMEYTTRYLEEAWMDSQLIYIDECGFNTWTSQKLGRNKKGERLYATLPTNRGRNMSLALAIGVNGPVHHKLVVGSYKKDTYQVFINEITVKLNGTGFRLIHDNASIHGGAISLNNVIEHLPPYSPFLNPIESVFSKIKFNIRKSISRAGGLSSMNFQSRFDFLKDALDNELVKEDYRDLTNYFWHIRRFFPKCIKKIHIFGDY 249
[Close]
BLAST of DDE_3 domain-containing protein vs. Planmine SMEST
Match: SMESG000050992.1 (SMESG000050992.1)

HSP 1 Score: 209.149 bits (531), Expect = 3.119e-59
Identity = 103/225 (45.78%), Postives = 149/225 (66.22%), Query Frame = 1 
Query:  265 KSISQTDKMKLVEAMEKGEDVFKLARQMGIGRNTAKSIIYVFKKTGKIFQGK-RLKNPNISEELTQKLLNFVDKNYWLTLEEIRKHLVDQNYEISVYCVSRCLRRNIVAFNKFRSHIAERNSEKVLELRKSYVRRLLEEEWNDNKMIYIDECYFNTWGSKTLSRTKMG-----VLPAALVPNICFVIAIGVKGIIHHKLLIGSLDKESYHVFINELTVKLNGTGY 921
            + IS+ DK++++ AME GED F   + +GI RN+ K++IY ++K GK+ QGK       ISE+   KLL+FV+KN + +LEE++  L+ +N  +S   +SR L  +++   + R  +AERNS++VL+LR  Y  R LEE W D+++IYIDEC FN W S+ L R K G      LP     N+   +AIGV G +HHKL++GS  K++Y +FINE+TVKLNGTGY
Sbjct:  963 RRISEIDKIRIINAMENGEDYFHFGKLLGIFRNSIKTLIYKYRKFGKLSQGKIGGSKSKISEDYGNKLLDFVEKNVYASLEEMKNFLIFENLSVSTNTISRFLGNHLITTKQIRPSVAERNSDRVLDLRMKYTTRYLEEAWMDSQLIYIDECGFNIWTSQKLGRNKKGERLYSTLPTNRGRNMSLALAIGVNGSVHHKLIVGSYKKDTYQIFINEITVKLNGTGY 1187
The following BLAST results are available for this feature:
BLAST of DDE_3 domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DDE_3 domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DDE_3 domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DDE_3 domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DDE_3 domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DDE_3 domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DDE_3 domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DDE_3 domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
W4XP448.816e-2825.91DDE_3 domain-containing protein OS=Strongylocentro... [more]
W4Z6F75.150e-2725.91DDE_3 domain-containing protein OS=Strongylocentro... [more]
A0A267DQJ81.651e-2625.94DDE_3 domain-containing protein OS=Macrostomum lig... [more]
W4Z4741.136e-2324.72DDE_3 domain-containing protein OS=Strongylocentro... [more]
A0A0C2JI121.222e-2227.67DDE_3 domain-containing protein OS=Thelohanellus k... [more]
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BLAST of DDE_3 domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DDE_3 domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DDE_3 domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DDE_3 domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DDE_3 domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DDE_3 domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000041908.17.090e-9348.14SMESG000041908.1[more]
SMESG000005064.11.116e-9045.00SMESG000005064.1[more]
SMESG000005064.15.091e-8343.99SMESG000005064.1[more]
SMESG000069530.14.521e-7748.59SMESG000069530.1[more]
SMESG000050992.13.119e-5945.78SMESG000050992.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30028299 ID=SMED30028299|Name=DDE_3 domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1274bp
ATCATTCTTTAGGTGCTGTTTAAGTCCATTGGGGCAATTAAATGTCCTTG
ACGTCGACAAAGAAACACACAAACACAACTAGCATACATGCACAATGACG
TGTACACACATTACAATCAAAGGCAATCAATTGGATGCATATGGATGTAT
GATTGTTTTAGTTTTGATTAATTTTAACTTGTAAAAATAAAAATGGATAC
TAGTGATGAAATATCTGATGCTTCGAGTGATAAAACGGTGCCTTTCCGAG
AAAGACCAGCTAAAAAATCAATATCACAAACGGATAAAATGAAACTTGTG
GAAGCCATGGAAAAGGGCGAAGACGTTTTTAAATTAGCAAGACAAATGGG
AATCGGGCGTAATACAGCAAAAAGTATAATTTATGTTTTTAAAAAAACTG
GAAAGATATTCCAGGGAAAACGACTCAAAAATCCAAATATTTCAGAAGAA
CTTACTCAAAAGTTGCTGAATTTTGTCGATAAAAATTATTGGCTAACTTT
AGAAGAAATTCGCAAGCATTTAGTTGATCAAAATTACGAAATTTCCGTCT
ATTGTGTTTCGAGATGTCTGAGAAGGAATATCGTAGCTTTCAATAAATTT
CGCTCACATATCGCAGAAAGAAATTCAGAAAAAGTTTTAGAACTTCGAAA
ATCCTATGTTCGACGCCTGTTGGAAGAAGAATGGAATGACAATAAAATGA
TTTACATTGATGAATGTTACTTCAACACTTGGGGCAGTAAAACTTTGAGT
AGAACCAAAATGGGAGTCCTTCCAGCAGCCCTCGTTCCAAATATATGTTT
CGTCATCGCCATTGGAGTCAAAGGAATAATTCATCACAAATTGTTAATTG
GGTCTTTGGATAAGGAAAGTTATCATGTTTTTATAAATGAGTTAACTGTC
AAATTGAATGGAACGGGCTATATTCTTATCCATGATAATGTTAAAAGTTA
TCGAGAAAACTTTTCTACTGATAATCAAACGGTAAATTTGCCTCCTTATA
GTTCATTTCTTAGTCCACTTGATGAAGTCTTCACTAAAATCAAGGGAAAC
GTCAGACAATCTTTGTCCCACATAAAGGAATTCACTTCAATGAATATAGA
CAATCGATTTGTATTCCTGAATGATGCTATCAACAATGAGCTAAAGAAAG
AAGATTATTCCAACTTAAAAATCTATTTCAAACGGATTAGAACATTTTTC
CCAAAAGCCATTTTAAAAGAAGAAATTTCTGGATTTTATTGATTTTATAA
ATAAATGTATTTTATCGAAAAAAA
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protein sequence of SMED30028299-orf-1

>SMED30028299-orf-1 ID=SMED30028299-orf-1|Name=SMED30028299-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=350bp
MDTSDEISDASSDKTVPFRERPAKKSISQTDKMKLVEAMEKGEDVFKLAR
QMGIGRNTAKSIIYVFKKTGKIFQGKRLKNPNISEELTQKLLNFVDKNYW
LTLEEIRKHLVDQNYEISVYCVSRCLRRNIVAFNKFRSHIAERNSEKVLE
LRKSYVRRLLEEEWNDNKMIYIDECYFNTWGSKTLSRTKMGVLPAALVPN
ICFVIAIGVKGIIHHKLLIGSLDKESYHVFINELTVKLNGTGYILIHDNV
KSYRENFSTDNQTVNLPPYSSFLSPLDEVFTKIKGNVRQSLSHIKEFTSM
NIDNRFVFLNDAINNELKKEDYSNLKIYFKRIRTFFPKAILKEEISGFY*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000020protonephridia
PLANA:0000204ovary
PLANA:0000429neoblast
PLANA:0002089reproductive organ
PLANA:0002109X1 cell
Vocabulary: INTERPRO
TermDefinition
IPR009057Homeobox-like_sf
Vocabulary: molecular function
TermDefinition
GO:0003677DNA binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 7..27
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..27
NoneNo IPR availablePANTHERPTHR23022:SF111coord: 28..296
NoneNo IPR availablePANTHERPTHR23022TRANSPOSABLE ELEMENT-RELATEDcoord: 28..296
IPR009057Homeobox-like domain superfamilySUPERFAMILYSSF46689Homeodomain-likecoord: 23..129