tRNA(His) guanylyltransferase

Overview
NametRNA(His) guanylyltransferase
Smed IDSMED30028269
Length (bp)1227
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of tRNA(His) guanylyltransferase (SMED30028269) t-SNE clustered cells

Violin plots show distribution of expression levels for tRNA(His) guanylyltransferase (SMED30028269) in cells (dots) of each of the 12 neoblast clusters.

 

back to top

 

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of tRNA(His) guanylyltransferase (SMED30028269) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for tRNA(His) guanylyltransferase (SMED30028269) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

back to top

 

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

back to top
Anatomical Expression

PAGE et. al., 2020



SMED30028269

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 1

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30028269SMESG000041162.1 SmedASXL_015955SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of tRNA(His) guanylyltransferase vs. Ensembl Human
Match: THG1L (tRNA-histidine guanylyltransferase 1 like [Source:HGNC Symbol;Acc:HGNC:26053])

HSP 1 Score: 276.174 bits (705), Expect = 2.952e-90
Identity = 131/267 (49.06%), Postives = 177/267 (66.29%), Query Frame = 1 
Query:   85 MAKSKFEYVKNFEELDNLLFETWIVCRIDGKNFHSFSTKHNFIKPNDINALMLMNEAAVSVISENTDIFMGYGQSDEFSFIFRSSATIFDRRKSKLLSTIVSKFSSSYVFYWKKYFPNQELLFPPSFDGRIVIYPKAGHVKDYLSWRQADCHINNLYNTCYWKLVLNENKTGKDAENELKDTNSATKNELLFTRFSTNYNNEPEIFRKGSILCRK---EYLSREQIL--ETNPTKCSAIRL------FHGDVISDKFWTTNPKLLSD 852
            MAKSKFEYV++FE  D  L   W+V R+DG+NFH F+ KHNF KPND  AL LM + A +V+ E  DI + YGQSDE+SF+F+     F RR SK ++ + S+F+SSYVFYW+ YF +Q LL+PP FDGR+V+YP    +KDYLSWRQADCHINNLYNT +W L+     T   A+  L+ T +A KNE+LF+ F+ NYNNE  ++RKG++L  +   E +++E  L  E    K +  R        H D+I D FW  +P++L +
Sbjct:   30 MAKSKFEYVRDFEADDTCLAHCWVVVRLDGRNFHRFAEKHNFAKPNDSRALQLMTKCAQTVMEELEDIVIAYGQSDEYSFVFKRKTNWFKRRASKFMTHVASQFASSYVFYWRDYFEDQPLLYPPGFDGRVVVYPSNQTLKDYLSWRQADCHINNLYNTVFWALIQQSGLTPVQAQGRLQGTLAADKNEILFSEFNINYNNELPMYRKGTVLIWQKVDEVMTKEIKLPTEMEGKKMAVTRTRTKPVPLHCDIIGDAFWKEHPEILDE 296
[Close]
BLAST of tRNA(His) guanylyltransferase vs. Ensembl Human
Match: THG1L (tRNA-histidine guanylyltransferase 1 like [Source:HGNC Symbol;Acc:HGNC:26053])

HSP 1 Score: 113.62 bits (283), Expect = 6.669e-30
Identity = 51/92 (55.43%), Postives = 64/92 (69.57%), Query Frame = 1 
Query:   85 MAKSKFEYVKNFEELDNLLFETWIVCRIDGKNFHSFSTKHNFIKPNDINALMLMNEAAVSVISENTDIFMGYGQSDEFSFIFRSSATIFDRR 360
            MAKSKFEYV++FE  D  L   W+V R+DG+NFH F+ KHNF KPND  AL LM + A +V+ E  DI + YGQSDE+SF+F+     F RR
Sbjct:   30 MAKSKFEYVRDFEADDTCLAHCWVVVRLDGRNFHRFAEKHNFAKPNDSRALQLMTKCAQTVMEELEDIVIAYGQSDEYSFVFKRKTNWFKRR 121
[Close]
BLAST of tRNA(His) guanylyltransferase vs. Ensembl Fly
Match: THG (gene:FBgn0283659 transcript:FBtr0303561)

HSP 1 Score: 268.855 bits (686), Expect = 6.878e-88
Identity = 133/255 (52.16%), Postives = 172/255 (67.45%), Query Frame = 1 
Query:   85 MAKSKFEYVKNFEELDNLLFETWIVCRIDGKNFHSFSTKHNFIKPNDINALMLMNEAAVSVISENTDIFMGYGQSDEFSFIFRSSATIFDRRKSKLLSTIVSKFSSSYVFYWKKYFPNQELLFPPSFDGRIVIYPKAGHVKDYLSWRQADCHINNLYNTCYWKLVLNENKTGKDAENELKDTNSATKNELLFTRFSTNYNNEPEIFRKGSILCRKEYLSREQILETNPTKCSAIRLFHGDVISDKFWTTNPKLLS 849
            MA S+FEYVK+FE+ D++L   WIV RIDGK FH FS  H+F KPND NAL +MN AA +V+ E  DI + YGQSDE+SF+FR     F RR +KLL+ + S FSSSYV  W K+  N  L + P FDGR+V+YP   ++KDYLSWRQAD H+NNLYNT +WKLVL +  T + AE +L+ T SA KNELLF  F  NYNN P ++RKG+IL RK  +  E+  +       A+   H D+IS +FW  + ++L 
Sbjct:    1 MACSRFEYVKSFEQDDSILPNVWIVIRIDGKKFHKFSKTHDFEKPNDENALNVMNAAATAVMQEFRDIVLAYGQSDEYSFVFRKETAAFKRRSAKLLTYVTSLFSSSYVMQWSKWM-NLPLAYAPCFDGRVVLYPSEQNLKDYLSWRQADVHVNNLYNTAFWKLVLEKGLTNQQAEAKLRGTFSADKNELLFQEFGINYNNLPAMYRKGTILLRKRVILGEKSRQ-------AVVPLHEDLISSQFWKEHTEILG 247
[Close]
BLAST of tRNA(His) guanylyltransferase vs. Ensembl Fly
Match: THG (gene:FBgn0283659 transcript:FBtr0080727)

HSP 1 Score: 268.855 bits (686), Expect = 6.878e-88
Identity = 133/255 (52.16%), Postives = 172/255 (67.45%), Query Frame = 1 
Query:   85 MAKSKFEYVKNFEELDNLLFETWIVCRIDGKNFHSFSTKHNFIKPNDINALMLMNEAAVSVISENTDIFMGYGQSDEFSFIFRSSATIFDRRKSKLLSTIVSKFSSSYVFYWKKYFPNQELLFPPSFDGRIVIYPKAGHVKDYLSWRQADCHINNLYNTCYWKLVLNENKTGKDAENELKDTNSATKNELLFTRFSTNYNNEPEIFRKGSILCRKEYLSREQILETNPTKCSAIRLFHGDVISDKFWTTNPKLLS 849
            MA S+FEYVK+FE+ D++L   WIV RIDGK FH FS  H+F KPND NAL +MN AA +V+ E  DI + YGQSDE+SF+FR     F RR +KLL+ + S FSSSYV  W K+  N  L + P FDGR+V+YP   ++KDYLSWRQAD H+NNLYNT +WKLVL +  T + AE +L+ T SA KNELLF  F  NYNN P ++RKG+IL RK  +  E+  +       A+   H D+IS +FW  + ++L 
Sbjct:    1 MACSRFEYVKSFEQDDSILPNVWIVIRIDGKKFHKFSKTHDFEKPNDENALNVMNAAATAVMQEFRDIVLAYGQSDEYSFVFRKETAAFKRRSAKLLTYVTSLFSSSYVMQWSKWM-NLPLAYAPCFDGRVVLYPSEQNLKDYLSWRQADVHVNNLYNTAFWKLVLEKGLTNQQAEAKLRGTFSADKNELLFQEFGINYNNLPAMYRKGTILLRKRVILGEKSRQ-------AVVPLHEDLISSQFWKEHTEILG 247
[Close]
BLAST of tRNA(His) guanylyltransferase vs. Ensembl Zebrafish
Match: thg1l (tRNA-histidine guanylyltransferase 1-like [Source:ZFIN;Acc:ZDB-GENE-041114-170])

HSP 1 Score: 270.781 bits (691), Expect = 2.381e-88
Identity = 136/264 (51.52%), Postives = 174/264 (65.91%), Query Frame = 1 
Query:   85 MAKSKFEYVKNFEELDNLLFETWIVCRIDGKNFHSFSTKHNFIKPNDINALMLMNEAAVSVISENTDIFMGYGQSDEFSFIFRSSATIFDRRKSKLLSTIVSKFSSSYVFYWKKYFPNQELLFPPSFDGRIVIYPKAGHVKDYLSWRQADCHINNLYNTCYWKLVLNENKTGKDAENELKDTNSATKNELLFTRFSTNYNNEPEIFRKGSIL---------CRKEYLSREQILETNPTKCSAIRLFHG-DVISDKFWTTNPKLL 846
            MAKSKFEYV+NFE  D  L   +IV R+DG+NFH FS +HNF KPND  AL LM+ +A SV+ E  DI + YGQSDEFSF+F+ S   F RR SKL++ + S+FSSS+VFYWK+YF  Q LL+PPSFDGR+V+YP   +V+DYLSWRQADCHINNLYNT +W LV     T   AE  L  T +  KNE+LF+ F+ NYNNE  +++KG+ L          ++     E+  E   T+       H  DVI D+FW  +P +L
Sbjct:   29 MAKSKFEYVRNFELDDTCLRNCYIVVRLDGRNFHKFSDQHNFTKPNDDRALGLMSRSACSVMEELDDITIAYGQSDEFSFVFKRSTNWFKRRASKLMTHVTSQFSSSFVFYWKEYFGEQPLLYPPSFDGRVVLYPSNRNVRDYLSWRQADCHINNLYNTAFWTLVQKGGLTTTQAEERLNGTLATDKNEILFSEFNINYNNESSVYKKGTTLIWEKVNETTTKQIKRPNEEETEVTVTRTRKKVTSHSCDVIGDQFWDEHPDIL 292
[Close]
BLAST of tRNA(His) guanylyltransferase vs. Ensembl Xenopus
Match: ENSXETT00000004129.1 (tRNA-histidine guanylyltransferase 1-like [Source:NCBI gene;Acc:100487878])

HSP 1 Score: 279.641 bits (714), Expect = 7.392e-92
Identity = 133/267 (49.81%), Postives = 185/267 (69.29%), Query Frame = 1 
Query:   85 MAKSKFEYVKNFEELDNLLFETWIVCRIDGKNFHSFSTKHNFIKPNDINALMLMNEAAVSVISENTDIFMGYGQSDEFSFIFRSSATIFDRRKSKLLSTIVSKFSSSYVFYWKKYFPNQELLFPPSFDGRIVIYPKAGHVKDYLSWRQADCHINNLYNTCYWKLVLNENKTGKDAENELKDTNSATKNELLFTRFSTNYNNEPEIFRKGSILCRKEY--LSREQI-----LETNPTKCSAIR----LFHGDVISDKFWTTNPKLLSD 852
            MAKSKFEYV++FE  D  L   W++ R+DG+NFH F+ +H+F KPND+ AL LMN  A +V+ E  DI + YGQSDE+SF+F   +  + RR SK ++ +VS+F+SSYVFYWK+YFP+  +L PP FDGR+V+YP   ++KDYLSWRQADCHINNLYNT +W LV     T   A++ LK T +A KNE+LF+ F+ NYNNEP ++RKGS+L  ++   +S+++I     ++    + S  R    + H DVI D+FW  +  +LSD
Sbjct:   19 MAKSKFEYVRDFEVQDTCLRNCWVLVRVDGRNFHRFAEQHHFTKPNDVRALQLMNRCAQNVMDELDDICLAYGQSDEYSFVFHKKSNWYKRRASKFMTHVVSQFASSYVFYWKEYFPDVPILCPPGFDGRVVLYPSEQNLKDYLSWRQADCHINNLYNTVFWSLVQKGGLTPAQAQDRLKGTLAAEKNEILFSEFNINYNNEPLLYRKGSVLIWQKVNEVSKKRIKLPNEVDEKEVEVSRWRKETVILHCDVIGDQFWEEHSSILSD 285
[Close]
BLAST of tRNA(His) guanylyltransferase vs. Ensembl Xenopus
Match: ENSXETT00000004233.1 (tRNA-histidine guanylyltransferase 1-like [Source:NCBI gene;Acc:100487878])

HSP 1 Score: 279.641 bits (714), Expect = 7.639e-92
Identity = 133/267 (49.81%), Postives = 185/267 (69.29%), Query Frame = 1 
Query:   85 MAKSKFEYVKNFEELDNLLFETWIVCRIDGKNFHSFSTKHNFIKPNDINALMLMNEAAVSVISENTDIFMGYGQSDEFSFIFRSSATIFDRRKSKLLSTIVSKFSSSYVFYWKKYFPNQELLFPPSFDGRIVIYPKAGHVKDYLSWRQADCHINNLYNTCYWKLVLNENKTGKDAENELKDTNSATKNELLFTRFSTNYNNEPEIFRKGSILCRKEY--LSREQI-----LETNPTKCSAIR----LFHGDVISDKFWTTNPKLLSD 852
            MAKSKFEYV++FE  D  L   W++ R+DG+NFH F+ +H+F KPND+ AL LMN  A +V+ E  DI + YGQSDE+SF+F   +  + RR SK ++ +VS+F+SSYVFYWK+YFP+  +L PP FDGR+V+YP   ++KDYLSWRQADCHINNLYNT +W LV     T   A++ LK T +A KNE+LF+ F+ NYNNEP ++RKGS+L  ++   +S+++I     ++    + S  R    + H DVI D+FW  +  +LSD
Sbjct:   20 MAKSKFEYVRDFEVQDTCLRNCWVLVRVDGRNFHRFAEQHHFTKPNDVRALQLMNRCAQNVMDELDDICLAYGQSDEYSFVFHKKSNWYKRRASKFMTHVVSQFASSYVFYWKEYFPDVPILCPPGFDGRVVLYPSEQNLKDYLSWRQADCHINNLYNTVFWSLVQKGGLTPAQAQDRLKGTLAAEKNEILFSEFNINYNNEPLLYRKGSVLIWQKVNEVSKKRIKLPNEVDEKEVEVSRWRKETVILHCDVIGDQFWEEHSSILSD 286
[Close]
BLAST of tRNA(His) guanylyltransferase vs. Ensembl Xenopus
Match: ENSXETT00000004245.1 (tRNA-histidine guanylyltransferase 1-like [Source:NCBI gene;Acc:100487878])

HSP 1 Score: 280.026 bits (715), Expect = 8.807e-92
Identity = 133/267 (49.81%), Postives = 185/267 (69.29%), Query Frame = 1 
Query:   85 MAKSKFEYVKNFEELDNLLFETWIVCRIDGKNFHSFSTKHNFIKPNDINALMLMNEAAVSVISENTDIFMGYGQSDEFSFIFRSSATIFDRRKSKLLSTIVSKFSSSYVFYWKKYFPNQELLFPPSFDGRIVIYPKAGHVKDYLSWRQADCHINNLYNTCYWKLVLNENKTGKDAENELKDTNSATKNELLFTRFSTNYNNEPEIFRKGSILCRKEY--LSREQI-----LETNPTKCSAIR----LFHGDVISDKFWTTNPKLLSD 852
            MAKSKFEYV++FE  D  L   W++ R+DG+NFH F+ +H+F KPND+ AL LMN  A +V+ E  DI + YGQSDE+SF+F   +  + RR SK ++ +VS+F+SSYVFYWK+YFP+  +L PP FDGR+V+YP   ++KDYLSWRQADCHINNLYNT +W LV     T   A++ LK T +A KNE+LF+ F+ NYNNEP ++RKGS+L  ++   +S+++I     ++    + S  R    + H DVI D+FW  +  +LSD
Sbjct:   35 MAKSKFEYVRDFEVQDTCLRNCWVLVRVDGRNFHRFAEQHHFTKPNDVRALQLMNRCAQNVMDELDDICLAYGQSDEYSFVFHKKSNWYKRRASKFMTHVVSQFASSYVFYWKEYFPDVPILCPPGFDGRVVLYPSEQNLKDYLSWRQADCHINNLYNTVFWSLVQKGGLTPAQAQDRLKGTLAAEKNEILFSEFNINYNNEPLLYRKGSVLIWQKVNEVSKKRIKLPNEVDEKEVEVSRWRKETVILHCDVIGDQFWEEHSSILSD 301
[Close]
BLAST of tRNA(His) guanylyltransferase vs. Ensembl Xenopus
Match: ENSXETT00000004158.1 (tRNA-histidine guanylyltransferase 1-like [Source:NCBI gene;Acc:100487878])

HSP 1 Score: 279.256 bits (713), Expect = 9.280e-92
Identity = 133/267 (49.81%), Postives = 185/267 (69.29%), Query Frame = 1 
Query:   85 MAKSKFEYVKNFEELDNLLFETWIVCRIDGKNFHSFSTKHNFIKPNDINALMLMNEAAVSVISENTDIFMGYGQSDEFSFIFRSSATIFDRRKSKLLSTIVSKFSSSYVFYWKKYFPNQELLFPPSFDGRIVIYPKAGHVKDYLSWRQADCHINNLYNTCYWKLVLNENKTGKDAENELKDTNSATKNELLFTRFSTNYNNEPEIFRKGSILCRKEY--LSREQI-----LETNPTKCSAIR----LFHGDVISDKFWTTNPKLLSD 852
            MAKSKFEYV++FE  D  L   W++ R+DG+NFH F+ +H+F KPND+ AL LMN  A +V+ E  DI + YGQSDE+SF+F   +  + RR SK ++ +VS+F+SSYVFYWK+YFP+  +L PP FDGR+V+YP   ++KDYLSWRQADCHINNLYNT +W LV     T   A++ LK T +A KNE+LF+ F+ NYNNEP ++RKGS+L  ++   +S+++I     ++    + S  R    + H DVI D+FW  +  +LSD
Sbjct:   18 MAKSKFEYVRDFEVQDTCLRNCWVLVRVDGRNFHRFAEQHHFTKPNDVRALQLMNRCAQNVMDELDDICLAYGQSDEYSFVFHKKSNWYKRRASKFMTHVVSQFASSYVFYWKEYFPDVPILCPPGFDGRVVLYPSEQNLKDYLSWRQADCHINNLYNTVFWSLVQKGGLTPAQAQDRLKGTLAAEKNEILFSEFNINYNNEPLLYRKGSVLIWQKVNEVSKKRIKLPNEVDEKEVEVSRWRKETVILHCDVIGDQFWEEHSSILSD 284
[Close]
BLAST of tRNA(His) guanylyltransferase vs. Ensembl Xenopus
Match: ENSXETT00000004339.1 (tRNA-histidine guanylyltransferase 1-like [Source:NCBI gene;Acc:100487878])

HSP 1 Score: 279.256 bits (713), Expect = 1.344e-91
Identity = 133/267 (49.81%), Postives = 185/267 (69.29%), Query Frame = 1 
Query:   85 MAKSKFEYVKNFEELDNLLFETWIVCRIDGKNFHSFSTKHNFIKPNDINALMLMNEAAVSVISENTDIFMGYGQSDEFSFIFRSSATIFDRRKSKLLSTIVSKFSSSYVFYWKKYFPNQELLFPPSFDGRIVIYPKAGHVKDYLSWRQADCHINNLYNTCYWKLVLNENKTGKDAENELKDTNSATKNELLFTRFSTNYNNEPEIFRKGSILCRKEY--LSREQI-----LETNPTKCSAIR----LFHGDVISDKFWTTNPKLLSD 852
            MAKSKFEYV++FE  D  L   W++ R+DG+NFH F+ +H+F KPND+ AL LMN  A +V+ E  DI + YGQSDE+SF+F   +  + RR SK ++ +VS+F+SSYVFYWK+YFP+  +L PP FDGR+V+YP   ++KDYLSWRQADCHINNLYNT +W LV     T   A++ LK T +A KNE+LF+ F+ NYNNEP ++RKGS+L  ++   +S+++I     ++    + S  R    + H DVI D+FW  +  +LSD
Sbjct:   26 MAKSKFEYVRDFEVQDTCLRNCWVLVRVDGRNFHRFAEQHHFTKPNDVRALQLMNRCAQNVMDELDDICLAYGQSDEYSFVFHKKSNWYKRRASKFMTHVVSQFASSYVFYWKEYFPDVPILCPPGFDGRVVLYPSEQNLKDYLSWRQADCHINNLYNTVFWSLVQKGGLTPAQAQDRLKGTLAAEKNEILFSEFNINYNNEPLLYRKGSVLIWQKVNEVSKKRIKLPNEVDEKEVEVSRWRKETVILHCDVIGDQFWEEHSSILSD 292
[Close]
BLAST of tRNA(His) guanylyltransferase vs. Ensembl Mouse
Match: Thg1l (tRNA-histidine guanylyltransferase 1-like (S. cerevisiae) [Source:MGI Symbol;Acc:MGI:1913878])

HSP 1 Score: 275.789 bits (704), Expect = 2.775e-90
Identity = 130/269 (48.33%), Postives = 175/269 (65.06%), Query Frame = 1 
Query:   85 MAKSKFEYVKNFEELDNLLFETWIVCRIDGKNFHSFSTKHNFIKPNDINALMLMNEAAVSVISENTDIFMGYGQSDEFSFIFRSSATIFDRRKSKLLSTIVSKFSSSYVFYWKKYFPNQELLFPPSFDGRIVIYPKAGHVKDYLSWRQADCHINNLYNTCYWKLVLNENKTGKDAENELKDTNSATKNELLFTRFSTNYNNEPEIFRKGSIL-------CRKEYLSREQILETNPTKCSAIR----LFHGDVISDKFWTTNPKLLSD*N 858
            MAKSKFEYV+NFE  D  L   W+V R+DG+NFH F+ +HNF KPND  AL LM + A +V+ E  DI + YGQSDE+SF+FR  +  F RR SK ++ + S+F+SSYVFYW+ YF +Q L +PP FDGR+V+YP    +KDYLSWRQADCHINNLYNT +W L+     T   A+  LK T +A KNE+LF+ F  NYNNEP ++RKG++L        R + +     +E      +  R      + D+I D FW  +P++L++ N
Sbjct:   30 MAKSKFEYVRNFEVQDTCLPHCWVVVRLDGRNFHRFAEEHNFAKPNDSRALHLMTKCAQTVMEELEDIVIAYGQSDEYSFVFRKKSNWFKRRASKFMTLVASQFASSYVFYWRDYFEDQPLRYPPGFDGRVVLYPSNQTLKDYLSWRQADCHINNLYNTVFWALIQQSGLTPVQAQQRLKGTLTADKNEILFSEFHINYNNEPHMYRKGTVLVWQKVEEVRTQEVRLPAEMEGEKKAVARTRTRVVALNCDLIGDAFWKEHPEILAEEN 298
[Close]
BLAST of tRNA(His) guanylyltransferase vs. Ensembl Mouse
Match: Thg1l (tRNA-histidine guanylyltransferase 1-like (S. cerevisiae) [Source:MGI Symbol;Acc:MGI:1913878])

HSP 1 Score: 228.409 bits (581), Expect = 1.360e-72
Identity = 108/234 (46.15%), Postives = 149/234 (63.68%), Query Frame = 1 
Query:  190 FSTKHNFIKPNDINALMLMNEAAVSVISENTDIFMGYGQSDEFSFIFRSSATIFDRRKSKLLSTIVSKFSSSYVFYWKKYFPNQELLFPPSFDGRIVIYPKAGHVKDYLSWRQADCHINNLYNTCYWKLVLNENKTGKDAENELKDTNSATKNELLFTRFSTNYNNEPEIFRKGSIL-------CRKEYLSREQILETNPTKCSAIR----LFHGDVISDKFWTTNPKLLSD*N 858
            F+ +HNF KPND  AL LM + A +V+ E  DI + YGQSDE+SF+FR  +  F RR SK ++ + S+F+SSYVFYW+ YF +Q L +PP FDGR+V+YP    +KDYLSWRQADCHINNLYNT +W L+     T   A+  LK T +A KNE+LF+ F  NYNNEP ++RKG++L        R + +     +E      +  R      + D+I D FW  +P++L++ N
Sbjct:    5 FAEEHNFAKPNDSRALHLMTKCAQTVMEELEDIVIAYGQSDEYSFVFRKKSNWFKRRASKFMTLVASQFASSYVFYWRDYFEDQPLRYPPGFDGRVVLYPSNQTLKDYLSWRQADCHINNLYNTVFWALIQQSGLTPVQAQQRLKGTLTADKNEILFSEFHINYNNEPHMYRKGTVLVWQKVEEVRTQEVRLPAEMEGEKKAVARTRTRVVALNCDLIGDAFWKEHPEILAEEN 238
[Close]
BLAST of tRNA(His) guanylyltransferase vs. UniProt/SwissProt
Match: sp|Q05B50|THG1_BOVIN (Probable tRNA(His) guanylyltransferase OS=Bos taurus OX=9913 GN=THG1L PE=2 SV=1)

HSP 1 Score: 276.944 bits (707), Expect = 7.645e-90
Identity = 130/267 (48.69%), Postives = 182/267 (68.16%), Query Frame = 1 
Query:   85 MAKSKFEYVKNFEELDNLLFETWIVCRIDGKNFHSFSTKHNFIKPNDINALMLMNEAAVSVISENTDIFMGYGQSDEFSFIFRSSATIFDRRKSKLLSTIVSKFSSSYVFYWKKYFPNQELLFPPSFDGRIVIYPKAGHVKDYLSWRQADCHINNLYNTCYWKLVLNENKTGKDAENELKDTNSATKNELLFTRFSTNYNNEPEIFRKGSILC--RKEYLSREQI---LETNPTKCSAIRL------FHGDVISDKFWTTNPKLLSD 852
            MAKSKFEYV++FE  D  L   W+V R+DG+NFH F+ KH+FIKPND  AL LM + A +V++E  DI + YGQSDE+SF+F+  +  F RR SK ++ +VS+F+SSYVFYW+ YF +Q LL+PP FDGR+++YP    +KDYLSWRQADCHINNLYNT +W LV     T   A+  L+ T +A KNE+LF+ F+ NYNNEP ++RKG++L   + E ++ +++    E    K +  R        H ++I D FW  +P++L +
Sbjct:   30 MAKSKFEYVRDFEADDTCLPHCWVVVRLDGRNFHRFAEKHSFIKPNDSRALHLMTKCAQTVMNELEDIVIAYGQSDEYSFVFKRKSNWFKRRASKFMTHVVSQFASSYVFYWRDYFEDQPLLYPPGFDGRVIVYPSNQTLKDYLSWRQADCHINNLYNTVFWALVQQSGLTPLQAQERLQGTLAADKNEILFSEFNINYNNEPLMYRKGTVLIWQKVEEITTKEVKLPAEMEGKKMAVTRTRTMVVPLHCNIIGDAFWKEHPEILDE 296
[Close]
BLAST of tRNA(His) guanylyltransferase vs. UniProt/SwissProt
Match: sp|Q9NWX6|THG1_HUMAN (Probable tRNA(His) guanylyltransferase OS=Homo sapiens OX=9606 GN=THG1L PE=1 SV=2)

HSP 1 Score: 276.174 bits (705), Expect = 1.418e-89
Identity = 131/267 (49.06%), Postives = 177/267 (66.29%), Query Frame = 1 
Query:   85 MAKSKFEYVKNFEELDNLLFETWIVCRIDGKNFHSFSTKHNFIKPNDINALMLMNEAAVSVISENTDIFMGYGQSDEFSFIFRSSATIFDRRKSKLLSTIVSKFSSSYVFYWKKYFPNQELLFPPSFDGRIVIYPKAGHVKDYLSWRQADCHINNLYNTCYWKLVLNENKTGKDAENELKDTNSATKNELLFTRFSTNYNNEPEIFRKGSILCRK---EYLSREQIL--ETNPTKCSAIRL------FHGDVISDKFWTTNPKLLSD 852
            MAKSKFEYV++FE  D  L   W+V R+DG+NFH F+ KHNF KPND  AL LM + A +V+ E  DI + YGQSDE+SF+F+     F RR SK ++ + S+F+SSYVFYW+ YF +Q LL+PP FDGR+V+YP    +KDYLSWRQADCHINNLYNT +W L+     T   A+  L+ T +A KNE+LF+ F+ NYNNE  ++RKG++L  +   E +++E  L  E    K +  R        H D+I D FW  +P++L +
Sbjct:   30 MAKSKFEYVRDFEADDTCLAHCWVVVRLDGRNFHRFAEKHNFAKPNDSRALQLMTKCAQTVMEELEDIVIAYGQSDEYSFVFKRKTNWFKRRASKFMTHVASQFASSYVFYWRDYFEDQPLLYPPGFDGRVVVYPSNQTLKDYLSWRQADCHINNLYNTVFWALIQQSGLTPVQAQGRLQGTLAADKNEILFSEFNINYNNELPMYRKGTVLIWQKVDEVMTKEIKLPTEMEGKKMAVTRTRTKPVPLHCDIIGDAFWKEHPEILDE 296
[Close]
BLAST of tRNA(His) guanylyltransferase vs. UniProt/SwissProt
Match: sp|Q9CY52|THG1_MOUSE (Probable tRNA(His) guanylyltransferase OS=Mus musculus OX=10090 GN=Thg1l PE=1 SV=1)

HSP 1 Score: 275.789 bits (704), Expect = 1.941e-89
Identity = 130/269 (48.33%), Postives = 175/269 (65.06%), Query Frame = 1 
Query:   85 MAKSKFEYVKNFEELDNLLFETWIVCRIDGKNFHSFSTKHNFIKPNDINALMLMNEAAVSVISENTDIFMGYGQSDEFSFIFRSSATIFDRRKSKLLSTIVSKFSSSYVFYWKKYFPNQELLFPPSFDGRIVIYPKAGHVKDYLSWRQADCHINNLYNTCYWKLVLNENKTGKDAENELKDTNSATKNELLFTRFSTNYNNEPEIFRKGSIL-------CRKEYLSREQILETNPTKCSAIR----LFHGDVISDKFWTTNPKLLSD*N 858
            MAKSKFEYV+NFE  D  L   W+V R+DG+NFH F+ +HNF KPND  AL LM + A +V+ E  DI + YGQSDE+SF+FR  +  F RR SK ++ + S+F+SSYVFYW+ YF +Q L +PP FDGR+V+YP    +KDYLSWRQADCHINNLYNT +W L+     T   A+  LK T +A KNE+LF+ F  NYNNEP ++RKG++L        R + +     +E      +  R      + D+I D FW  +P++L++ N
Sbjct:   30 MAKSKFEYVRNFEVQDTCLPHCWVVVRLDGRNFHRFAEEHNFAKPNDSRALHLMTKCAQTVMEELEDIVIAYGQSDEYSFVFRKKSNWFKRRASKFMTLVASQFASSYVFYWRDYFEDQPLRYPPGFDGRVVLYPSNQTLKDYLSWRQADCHINNLYNTVFWALIQQSGLTPVQAQQRLKGTLTADKNEILFSEFHINYNNEPHMYRKGTVLVWQKVEEVRTQEVRLPAEMEGEKKAVARTRTRVVALNCDLIGDAFWKEHPEILAEEN 298
[Close]
BLAST of tRNA(His) guanylyltransferase vs. UniProt/SwissProt
Match: sp|Q5M965|THG1_RAT (Probable tRNA(His) guanylyltransferase OS=Rattus norvegicus OX=10116 GN=Thg1l PE=2 SV=1)

HSP 1 Score: 275.404 bits (703), Expect = 2.464e-89
Identity = 130/265 (49.06%), Postives = 173/265 (65.28%), Query Frame = 1 
Query:   85 MAKSKFEYVKNFEELDNLLFETWIVCRIDGKNFHSFSTKHNFIKPNDINALMLMNEAAVSVISENTDIFMGYGQSDEFSFIFRSSATIFDRRKSKLLSTIVSKFSSSYVFYWKKYFPNQELLFPPSFDGRIVIYPKAGHVKDYLSWRQADCHINNLYNTCYWKLVLNENKTGKDAENELKDTNSATKNELLFTRFSTNYNNEPEIFRKGSIL-------CRKEYLSREQILETNPTKCSAIR----LFHGDVISDKFWTTNPKLL 846
            MAKSKFEYV++FE  D  L   W+V R+DG+NFH F+ KHNF KPND  AL LM + A +V+ E  DI + YGQSDE+SF+FR  +  F RR SK ++ + S+F+SSYVFYW+ YF +Q LL+PP FDGR+V+YP    +KDYLSWRQADCHINNLYNT +W L+     T   A+  LK T +A KNE+LF+ F  NYNNEP ++RKG++L        R + +     +E      +  R      + D+I D FW  +P++L
Sbjct:   30 MAKSKFEYVRDFEVDDTCLPHCWVVVRLDGRNFHRFAEKHNFAKPNDSRALHLMTKCAQTVMQELEDIVIAYGQSDEYSFVFRKRSNWFKRRASKFMTLVASQFASSYVFYWRDYFEDQPLLYPPGFDGRVVLYPSNQTLKDYLSWRQADCHINNLYNTVFWALIQQSGLTPVQAQQRLKGTLTADKNEILFSEFHINYNNEPHMYRKGTVLVWQKVNEVRTQEIRLPAEMEGEKMAVTRTRTKLVALNCDLIGDAFWKEHPEIL 294
[Close]
BLAST of tRNA(His) guanylyltransferase vs. UniProt/SwissProt
Match: sp|Q9V3N8|THG1_DROME (Probable tRNA(His) guanylyltransferase OS=Drosophila melanogaster OX=7227 GN=THG PE=1 SV=1)

HSP 1 Score: 268.855 bits (686), Expect = 6.895e-87
Identity = 133/255 (52.16%), Postives = 172/255 (67.45%), Query Frame = 1 
Query:   85 MAKSKFEYVKNFEELDNLLFETWIVCRIDGKNFHSFSTKHNFIKPNDINALMLMNEAAVSVISENTDIFMGYGQSDEFSFIFRSSATIFDRRKSKLLSTIVSKFSSSYVFYWKKYFPNQELLFPPSFDGRIVIYPKAGHVKDYLSWRQADCHINNLYNTCYWKLVLNENKTGKDAENELKDTNSATKNELLFTRFSTNYNNEPEIFRKGSILCRKEYLSREQILETNPTKCSAIRLFHGDVISDKFWTTNPKLLS 849
            MA S+FEYVK+FE+ D++L   WIV RIDGK FH FS  H+F KPND NAL +MN AA +V+ E  DI + YGQSDE+SF+FR     F RR +KLL+ + S FSSSYV  W K+  N  L + P FDGR+V+YP   ++KDYLSWRQAD H+NNLYNT +WKLVL +  T + AE +L+ T SA KNELLF  F  NYNN P ++RKG+IL RK  +  E+  +       A+   H D+IS +FW  + ++L 
Sbjct:    1 MACSRFEYVKSFEQDDSILPNVWIVIRIDGKKFHKFSKTHDFEKPNDENALNVMNAAATAVMQEFRDIVLAYGQSDEYSFVFRKETAAFKRRSAKLLTYVTSLFSSSYVMQWSKWM-NLPLAYAPCFDGRVVLYPSEQNLKDYLSWRQADVHVNNLYNTAFWKLVLEKGLTNQQAEAKLRGTFSADKNELLFQEFGINYNNLPAMYRKGTILLRKRVILGEKSRQ-------AVVPLHEDLISSQFWKEHTEILG 247
[Close]
BLAST of tRNA(His) guanylyltransferase vs. TrEMBL
Match: A0A397G9M9 (tRNA(His) guanylyltransferase OS=Diversispora epigaea OX=1348612 GN=Glove_668g6 PE=3 SV=1)

HSP 1 Score: 302.368 bits (773), Expect = 5.033e-98
Identity = 146/256 (57.03%), Postives = 189/256 (73.83%), Query Frame = 1 
Query:   85 MAKSKFEYVKNFEELDNLLFETWIVCRIDGKNFHSFSTKHNFIKPNDINALMLMNEAAVSVISENTDIFMGYGQSDEFSFIFRSSATIFDRRKSKLLSTIVSKFSSSYVFYWKKYFPNQELLFPPSFDGRIVIYPKAGHVKDYLSWRQADCHINNLYNTCYWKLVLNENKTGKDAENELKDTNSATKNELLFTRFSTNYNNEPEIFRKGSILCRKEYLSR---EQILETNPTKCSAIRLFHGDVISDKFWTTNPKL 843
            MAKSK+EYVK FE+ D LL  TW+V RIDG+ FH FS +H F KPND +AL LMN+ AVSV+++  DI + YGQSDE+SF+ +    ++ RR++K++STIVS F+S+YVFYWK YFP +ELL+PPSFDGR V+YP   + +DYLSWRQ DCHINNLYNTC+W LV++  KT  +AEN L+ T S+ KNELLF++F  NYNN PE+FRKGS++ RKE   +   E  +E    K  A+ + H D+I DKFW  NP L
Sbjct:    1 MAKSKYEYVKQFEKNDVLLPNTWLVVRIDGRGFHKFSDQHKFKKPNDQDALNLMNKCAVSVMNDIHDIVLAYGQSDEYSFVLKKDCALYGRRETKIVSTIVSLFTSNYVFYWKNYFPQKELLYPPSFDGRAVLYPADNNFRDYLSWRQVDCHINNLYNTCFWALVIS-GKTESEAENALRGTISSEKNELLFSQFKINYNNLPEMFRKGSVIIRKEVDVKTTCENGMEVTRKKKVAL-ILHDDIIGDKFWKENPTL 254
[Close]
BLAST of tRNA(His) guanylyltransferase vs. TrEMBL
Match: C3Y492 (tRNA(His) guanylyltransferase OS=Branchiostoma floridae OX=7739 GN=BRAFLDRAFT_60263 PE=3 SV=1)

HSP 1 Score: 293.508 bits (750), Expect = 2.617e-94
Identity = 139/268 (51.87%), Postives = 182/268 (67.91%), Query Frame = 1 
Query:   85 MAKSKFEYVKNFEELDNLLFETWIVCRIDGKNFHSFSTKHNFIKPNDINALMLMNEAAVSVISENTDIFMGYGQSDEFSFIFRSSATIFDRRKSKLLSTIVSKFSSSYVFYWKKYFPNQELLFPPSFDGRIVIYPKAGHVKDYLSWRQADCHINNLYNTCYWKLVLNENKTGKDAENELKDTNSATKNELLFTRFSTNYNNEPEIFRKGSIL------------CRKEYLSREQILETNPTKCSAIRLFHGDVISDKFWTTNPKLLSD 852
            MAKSKFEYV+ FE  D  L  TWIV R+DG+NFH FST H F KPND  AL LMN AA +V+++  DI + YGQSDEFSF+ + S  ++ RR SKL++ IVS+FSSSYVF+W ++FP+Q L +PP FDGR+++YP   +++DYLSWRQADCHINNLYNTC+W LV     T K AE  ++ T SA KNE+LF+ F  NYNNEPE+++KG++L            CR E    E+  E    +   + L H D+I D FW  +P +L +
Sbjct:    1 MAKSKFEYVRQFETQDPCLPNTWIVIRLDGRNFHRFSTDHGFTKPNDERALQLMNRAAETVMNDFRDIVISYGQSDEFSFVLKKSTMLYSRRASKLMTHIVSQFSSSYVFHWAQHFPDQPLQYPPGFDGRVILYPSNKNLRDYLSWRQADCHINNLYNTCFWALVQQGGMTNKQAEERIRHTFSADKNEILFSEFGINYNNEPEMYKKGTVLVWKRVEETVMKACRTEDDPVERPREVTKLRKKVVPL-HTDIIGDVFWEEHPDILEE 267
[Close]
BLAST of tRNA(His) guanylyltransferase vs. TrEMBL
Match: A0A3M6TSQ6 (tRNA(His) guanylyltransferase OS=Pocillopora damicornis OX=46731 GN=pdam_00010053 PE=3 SV=1)

HSP 1 Score: 291.197 bits (744), Expect = 1.218e-93
Identity = 138/254 (54.33%), Postives = 181/254 (71.26%), Query Frame = 1 
Query:   85 MAKSKFEYVKNFEELDNLLFETWIVCRIDGKNFHSFSTKHNFIKPNDINALMLMNEAAVSVISENTDIFMGYGQSDEFSFIFRSSATIFDRRKSKLLSTIVSKFSSSYVFYWKKYFPNQELLFPPSFDGRIVIYPKAGHVKDYLSWRQADCHINNLYNTCYWKLVLNENKTGKDAENELKDTNSATKNELLFTRFSTNYNNEPEIFRKGSILCRKEYLSREQILETNPTKCSAIRLFHGDVISDKFWTTNPKLL 846
            MAKSK+EYVK FE+ D  L   WIV RIDG+NFH FS  H+F KPND  AL LMN+ A +V+ E TDI + YGQSDE+S +F+ S T F RR SKL++ +V+ FSSSYVFYW ++FP QELL+PP FDGR+V+YP   +++DY+SWRQADCHINNLYNTC+W LV     T ++AE  L  T S+ KNELLF+ F+ NYNN P+I+RKGS+L      + ++     P +   I + H D+I D FW+ +P++L
Sbjct:    1 MAKSKYEYVKKFEQNDQCLMNCWIVIRIDGRNFHRFSDGHSFEKPNDRRALQLMNKCAETVMKEFTDIVIAYGQSDEYSLVFKRSTTQFSRRASKLVTNVVTLFSSSYVFYWNQFFPTQELLYPPMFDGRVVLYPSEKNLRDYMSWRQADCHINNLYNTCFWCLVKQSGLTVEEAEERLNGTVSSDKNELLFSVFNMNYNNLPQIYRKGSVLVCDINDTAQEENARKPKR--QIVVHHTDIIGDTFWSEHPEIL 252
[Close]
BLAST of tRNA(His) guanylyltransferase vs. TrEMBL
Match: A0A4X2K1V2 (tRNA(His) guanylyltransferase OS=Vombatus ursinus OX=29139 GN=THG1L PE=3 SV=1)

HSP 1 Score: 290.426 bits (742), Expect = 1.116e-92
Identity = 138/267 (51.69%), Postives = 182/267 (68.16%), Query Frame = 1 
Query:   85 MAKSKFEYVKNFEELDNLLFETWIVCRIDGKNFHSFSTKHNFIKPNDINALMLMNEAAVSVISENTDIFMGYGQSDEFSFIFRSSATIFDRRKSKLLSTIVSKFSSSYVFYWKKYFPNQELLFPPSFDGRIVIYPKAGHVKDYLSWRQADCHINNLYNTCYWKLVLNENKTGKDAENELKDTNSATKNELLFTRFSTNYNNEPEIFRKGSILCR---KEYLSREQIL--ETNPTKCSAIR------LFHGDVISDKFWTTNPKLLSD 852
            MAKSKFEYV++FE  D  L   W+V R+DG+NFH F+ KHNF KPND  AL LM + A +V+++  DI M YGQSDE+SF+F+  +  F RR SK ++ + S+F+SSYVFYWK YF +QELL+PP+FDGR+V+YP    +KDYLSWRQADCHINNLYNT +W L+     T   A+  L+ T +A KNE+LF+ ++ NYNNEP ++RKG+IL     KE +S+E  L  ET   K    R        H D+I D FW  +P++L D
Sbjct:   32 MAKSKFEYVRDFEADDTCLAHCWVVVRLDGRNFHRFAEKHNFAKPNDSRALELMTQCAQTVMTQLEDIVMAYGQSDEYSFVFKRKSNWFRRRASKFMTNVASQFASSYVFYWKDYFKDQELLYPPAFDGRVVVYPSNQTLKDYLSWRQADCHINNLYNTVFWMLIQRSKLTPAQAQERLQGTLAADKNEILFSEYNINYNNEPPMYRKGTILIWKKIKEVISKEIKLPGETEEKKVEVTRTRTKPVALHCDIIGDAFWKEHPEILED 298
[Close]
BLAST of tRNA(His) guanylyltransferase vs. TrEMBL
Match: G3Q3D5 (tRNA(His) guanylyltransferase OS=Gasterosteus aculeatus OX=69293 PE=3 SV=1)

HSP 1 Score: 288.886 bits (738), Expect = 4.013e-92
Identity = 140/266 (52.63%), Postives = 182/266 (68.42%), Query Frame = 1 
Query:   85 MAKSKFEYVKNFEELDNLLFETWIVCRIDGKNFHSFSTKHNFIKPNDINALMLMNEAAVSVISENTDIFMGYGQSDEFSFIFRSSATIFDRRKSKLLSTIVSKFSSSYVFYWKKYFPNQELLFPPSFDGRIVIYPKAGHVKDYLSWRQADCHINNLYNTCYWKLVLNENKTGKDAENELKDTNSATKNELLFTRFSTNYNNEPEIFRKGSILC---RKEYLSREQILETNPTKCSA-------IRLFHGDVISDKFWTTNPKLLSD 852
            MAKSKFEYV+NFE  D  L   +IV R+DG+NFH F+ KH F+KPND  AL LM  +A SV+ E  DI + YGQSDEFSF+F+ S++ F RR SKL++ + S+FSSSYVFYWK++F  Q LL+PP FDGR+V+YP   +++DYLSWRQADCHINNLYNT +W LV     T   AE+ LK T +A KNE+LF+ F  NYN EP + RKG+ L    R E +++ Q L T   K  A       ++  HGD+I ++FW  +P +L D
Sbjct:   30 MAKSKFEYVRNFETDDTCLRNCYIVVRLDGRNFHKFAEKHKFMKPNDNRALDLMTRSARSVMEELEDIVIAYGQSDEFSFVFKRSSSWFKRRSSKLMTHVASQFSSSYVFYWKEFFGEQPLLYPPGFDGRVVLYPSNRNLRDYLSWRQADCHINNLYNTVFWTLVQKGGLTTHQAEDRLKGTLAADKNEILFSEFDINYNTEPALHRKGTTLIWEKRDETVTKRQKLPTGEEKEVAVTRSRRSVQAHHGDIIGEQFWEEHPDVLED 295
[Close]
BLAST of tRNA(His) guanylyltransferase vs. Ensembl Cavefish
Match: thg1l (tRNA-histidine guanylyltransferase 1 like [Source:NCBI gene;Acc:103044514])

HSP 1 Score: 264.233 bits (674), Expect = 6.684e-86
Identity = 131/266 (49.25%), Postives = 175/266 (65.79%), Query Frame = 1 
Query:   85 MAKSKFEYVKNFEELDNLLFETWIVCRIDGKNFHSFSTKHNFIKPNDINALMLMNEAAVSVISENTDIFMGYGQSDEFSFIFRSSATIFDRRKSKLLSTIVSKFSSSYVFYWKKYFPNQELLFPPSFDGRIVIYPKAGHVKDYLSWRQADCHINNLYNTCYWKLVLNENKTGKDAENELKDTNSATKNELLFTRFSTNYNNEPEIFRKGSIL---------CRKEYLSREQILETNPTKC-SAIRLFHGDVISDKFWTTNPKLLSD 852
            MAKSK+EYV+NFE  D  L   +IV R+DG+NFH F+ +HNF KPND  AL LM+ +A SV+ +  DI + YGQSDEFSF+F+ S   F RR SKL++ + S+FSSSYVF+WK+YF  Q LL+PP FDGR+V+YP   +++DYLSWRQADCHINNLYNT +W LV     +   AE  L  T S  KNE+LF+ F+ NYNNE  + RKG+ L          +K  + +E   E   T+    +   + D+I+D FW  +P +L +
Sbjct:   30 MAKSKYEYVRNFETDDTCLRNCYIVVRLDGRNFHKFADQHNFTKPNDDRALGLMSRSARSVMEDLEDIAIAYGQSDEFSFVFKKSTNWFKRRASKLMTHVASQFSSSYVFFWKEYFGEQPLLYPPGFDGRVVLYPSNRNLRDYLSWRQADCHINNLYNTAFWTLVQKGGLSKTQAEERLNGTLSGDKNEILFSEFNLNYNNECALHRKGTTLIWEKVDETSTKKITVPKEGEKEVTFTRTRRKVTAHYCDIIADSFWNEHPDILEE 295
[Close]
BLAST of tRNA(His) guanylyltransferase vs. Ensembl Sea Lamprey
Match: thg1l (tRNA-histidine guanylyltransferase 1-like [Source:ZFIN;Acc:ZDB-GENE-041114-170])

HSP 1 Score: 219.935 bits (559), Expect = 1.747e-70
Identity = 100/202 (49.50%), Postives = 132/202 (65.35%), Query Frame = 1 
Query:  148 TWIVCRIDGKNFHSFSTKHNFIKPNDINALMLMNEAAVSVISENTDIFMGYGQSDEFSFIFRSSATIFDRRKSKLLSTIVSKFSSSYVFYWKKYFPNQELLFPPSFDGRIVIYPKAGHVKDYLSWRQADCHINNLYNTCYWKLVLNENKTGKDAENELKDTNSATKNELLFTRFSTNYNNEPEIFRKGSILCRKEYLSREQI 753
            TW+V  +       F+  H F+KPND   L LM   A  V++E  D+ + YGQSDEFSF+F      F RR SK ++ + S FSS++VFYW K+F  + LL+PP+FDGR+V+YP   H++DYLSWRQADCHINNLYNT +W LV N   T   A+  LK T +A KNE+LF+ F  NYN EP  FRKG+ +  ++ L+R  I
Sbjct:    3 TWLVSAVSAPRCR-FAEDHGFVKPNDARGLRLMAHCAAHVVTELDDLILAYGQSDEFSFVFHRKTNWFKRRASKFMTQVASLFSSAFVFYWDKFFAPERLLYPPAFDGRVVLYPTERHLRDYLSWRQADCHINNLYNTVFWALVQNGGLTTTQAQERLKLTVAADKNEILFSEFGINYNKEPLQFRKGTTIIWEKVLARRPI 203
[Close]
BLAST of tRNA(His) guanylyltransferase vs. Ensembl Sea Lamprey
Match: thg1l (tRNA-histidine guanylyltransferase 1-like [Source:ZFIN;Acc:ZDB-GENE-041114-170])

HSP 1 Score: 214.927 bits (546), Expect = 1.619e-68
Identity = 97/191 (50.79%), Postives = 126/191 (65.97%), Query Frame = 1 
Query:  148 TWIVCRIDGKNFHSFSTKHNFIKPNDINALMLMNEAAVSVISENTDIFMGYGQSDEFSFIFRSSATIFDRRKSKLLSTIVSKFSSSYVFYWKKYFPNQELLFPPSFDGRIVIYPKAGHVKDYLSWRQADCHINNLYNTCYWKLVLNENKTGKDAENELKDTNSATKNELLFTRFSTNYNNEPEIFRKGSIL 720
            TW+V  +       F+  H F+KPND   L LM   A  V++E  D+ + YGQSDEFSF+F      F RR SK ++ + S FSS++VFYW K+F  + LL+PP+FDGR+V+YP   H++DYLSWRQADCHINNLYNT +W LV N   T   A+  LK T +A KNE+LF+ F  NYN EP  FRKG+ +
Sbjct:   21 TWLVSAVSAPRCR-FAEDHGFVKPNDARGLRLMAHCAAHVVTELDDLILAYGQSDEFSFVFHRKTNWFKRRASKFMTQVASLFSSAFVFYWDKFFAPERLLYPPAFDGRVVLYPTERHLRDYLSWRQADCHINNLYNTVFWALVQNGGLTTTQAQERLKLTVAADKNEILFSEFGINYNKEPLQFRKGTTI 210
[Close]
BLAST of tRNA(His) guanylyltransferase vs. Ensembl Yeast
Match: THG1 (tRNAHis guanylyltransferase; adds a guanosine residue to the 5' end of tRNAH is after transcription and RNase P cleavage; can also catalyze reverse (3'-5') polymerization with certain substrates in a template-dependent reaction; couples nuclear division and migration to cell budding and cytokinesis; essential enzyme conserved among eukaryotes [Source:SGD;Acc:S000003256])

HSP 1 Score: 212.231 bits (539), Expect = 2.707e-67
Identity = 110/218 (50.46%), Postives = 144/218 (66.06%), Query Frame = 1 
Query:   85 MAKSKFEYVKNFEELDNLLFETWIVCRIDGKNFHSFSTKHNFIKPNDINALMLMNEAAVS-VISENTDIFMGYGQSDEFSFIFRSSATIFDRRKSKLLSTIVSKFSSSYVFYWKKYFPNQELLFP--PSFDGRIVIYPKAGHVKDYLSWRQADCHINNLYNTCYWKLVLNENKTGKDAENELKDTNSATKNELLFTRFSTNYNNEPEIFRKGSILCRK 729
            MA SKF YV+ FE  D +L + +IV RIDGK FH FS  + F KPND NAL LMN  A + V+    DI + +G+SDE+SFI +SS T+F+RRK KL +   S F+S+YV  W K+FP + L     P FD R V YP    +KDYLSWR  D HINNLYNT +W+L++    T +++E +L  T S  K E+LF+    NYNNEPE+F+KGS++ RK
Sbjct:    1 MANSKFGYVRQFETHDVILPQCYIVVRIDGKKFHEFSKFYEFAKPNDENALKLMNACAKNLVLKYKNDIILAFGESDEYSFILKSSTTLFNRRKDKLATLFGSFFTSNYVALWAKFFPEKPLNIKHLPYFDSRCVAYPNLQTIKDYLSWRYVDTHINNLYNTTFWQLIIKCGLTPQESEKKLCGTFSNEKQEILFSECGINYNNEPEMFKKGSLVTRK 218
[Close]
BLAST of tRNA(His) guanylyltransferase vs. Ensembl Nematostella
Match: EDO47952 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RKR9])

HSP 1 Score: 275.789 bits (704), Expect = 2.439e-91
Identity = 132/258 (51.16%), Postives = 174/258 (67.44%), Query Frame = 1 
Query:   85 MAKSKFEYVKNFEELDNLLFETWIVCRIDGKNFHSFSTKHNFIKPNDINALMLMNEAAVSVISENTDIFMGYGQSDEFSFIFRSSATIFDRRKSKLLSTIVSKFSSSYVFYWKKYFPNQELLFPPSFDGRIVIYPKAGHVKDYLSWRQADCHINNLYNTCYWKLVLNENKTGKDAENELKDTNSATKNELLFTRFSTNYNNEPEIFRKGSILC---RKEYLSREQILETNPTKCSAIRLFHGDVISDKFWTTNPKLLS 849
            MAKSK+EYV+ FE+ D  L   WIV R+DG+NFH FS  H F KPND   L LMN+ A +V++E  DI + YGQSDE+SF+FR + T F RR SKL++ +VS F+++YVF+W  +F +Q LL+PP FDGR+V+YP   +++DYLSWRQADCHINNLYNTC+W LV     T   AE  L  T S+ KNELLF+ F  NYNNEPE++RKGSIL    + E  + E   +        +   H D+I D FW  + ++L+
Sbjct:    1 MAKSKYEYVRKFEQNDACLPNCWIVVRVDGRNFHRFSDSHGFKKPNDPRGLGLMNKCAEAVMTEFGDIVICYGQSDEYSFVFRKNTTQFSRRVSKLITNVVSLFAATYVFHWASFFTDQHLLYPPMFDGRVVLYPSDKNLRDYLSWRQADCHINNLYNTCFWCLVNQGGVTQTKAEERLCGTVSSDKNELLFSEFDVNYNNEPELYRKGSILIWVQKNEASTEENTTDKKKRIRRKVTTLHTDIIGDGFWDEHREILT 258
[Close]
BLAST of tRNA(His) guanylyltransferase vs. Ensembl Nematostella
Match: EDO28396 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T6K8])

HSP 1 Score: 78.5666 bits (192), Expect = 5.236e-17
Identity = 56/194 (28.87%), Postives = 90/194 (46.39%), Query Frame = 1 
Query:  133 NLLFETWIVCRIDGKNFHSFSTKH-NFIKPNDINALMLMNEAAVSVISENTDIFMGYGQSDEFSFIFRSSATIFDRRKSKLLSTIVSKFSSSYVFYWKKYFPNQELLFPPSFDGRIVIYPKAGHVKDYLSWRQADCHINNLYNTCYWKLVLNENKTGKDAENELKDTNSATKNELLFTRFSTNYNNEPEIFRKG 711
            ++L + +IV R+DGK F   + +  +  KP DI     M      +++    +   Y QSDE S +       F+R+  K+ S +  + S+ +  Y+ K             D R +  P    + DY  WRQ D H N+L   CYW L  N N +  +A  + +  + + KNELLF +   NYN+ P   ++G
Sbjct:   11 HVLPDNYIVVRLDGKGFTKLTKESLDLEKPFDIRFHDAMVATTKHLLTVGFKVIYAYTQSDEISLLIDKDDNTFNRKVRKINSVLAGEASAFFSMYFNKL---------SVLDCRTICIPNIEMLLDYFCWRQEDAHRNSLSAYCYWTLRNNGN-SYIEATKKTEKLSVSDKNELLF-QHGINYNSVPSWQKRG 193
[Close]
BLAST of tRNA(His) guanylyltransferase vs. Ensembl Medaka
Match: thg1l (tRNA-histidine guanylyltransferase 1 like [Source:NCBI gene;Acc:101162300])

HSP 1 Score: 271.937 bits (694), Expect = 7.567e-89
Identity = 137/268 (51.12%), Postives = 181/268 (67.54%), Query Frame = 1 
Query:   85 MAKSKFEYVKNFEELDNLLFETWIVCRIDGKNFHSFSTKHNFIKPNDINALMLMNEAAVSVISENTDIFMGYGQSDEFSFIFRSSATIFDRRKSKLLSTIVSKFSSSYVFYWKKYFPNQELLFPPSFDGRIVIYPKAGHVKDYLSWRQADCHINNLYNTCYWKLVLNENKTGKDAENELKDTNSATKNELLFTRFSTNYNNEPEIFRKGSILCRKEYLSR--EQILETN------PTKCSAIRL--FHGDVISDKFWTTNPKLLSD*N 858
            MAKSKFEYV++FE  D  L   +IV R+DG+NFH FS +H F KPND  AL LM  +A SV+ E  DI + YGQSDEFSF+F+ ++  F RR SKL++ + S+FSSSYVFYWK+YF  Q LL+PPSFDGR+V+YP   +++DYLSWRQADCHINNLYNT +W LV     T   AE+ LK T +A KNE+LF+ F  NYNNE  + RKG+IL  K++      ++ + N      P   S  R+   + D+I ++FW  +P +L + N
Sbjct:   29 MAKSKFEYVRSFETDDTCLRNCYIVVRLDGRNFHKFSEQHGFTKPNDNRALGLMTRSAQSVMEELEDIIIAYGQSDEFSFVFKRTSNWFKRRASKLMTHVASQFSSSYVFYWKEYFEEQPLLYPPSFDGRVVLYPTNRNLRDYLSWRQADCHINNLYNTVFWTLVQKGGLTTAQAEDRLKGTLAADKNEILFSEFDINYNNESALHRKGTILLWKKHEETVIRRMKQPNAEEKEVPVTRSKRRVENLYCDIIGEQFWEEHPDILEEDN 296
[Close]
BLAST of tRNA(His) guanylyltransferase vs. Planmine SMEST
Match: SMESG000041162.1 (SMESG000041162.1)

HSP 1 Score: 518.464 bits (1334), Expect = 0.000e+0
Identity = 250/256 (97.66%), Postives = 252/256 (98.44%), Query Frame = 1 
Query:   85 MAKSKFEYVKNFEELDNLLFETWIVCRIDGKNFHSFSTKHNFIKPNDINALMLMNEAAVSVISENTDIFMGYGQSDEFSFIFRSSATIFDRRKSKLLSTIVSKFSSSYVFYWKKYFPNQELLFPPSFDGRIVIYPKAGHVKDYLSWRQADCHINNLYNTCYWKLVLNENKTGKDAENELKDTNSATKNELLFTRFSTNYNNEPEIFRKGSILCRKEYLSREQILETNPTKCSAIRLFHGDVISDKFWTTNPKLLSD 852
            MAKSKFEYVKNFEELDNLL+ETWIVCRIDGKNFHSFSTKHNFIKPNDINALMLMNEAAVSVISENTDIFMGYGQSDEFSFIF SSATIFDRRKSKLLSTIVSKFSSSYVFYWKKYFPNQELLFPPSFDGRIVIYPKAGHVKDYLSWRQADCHINNLYNTCYWK+VLNENKTGKDAENELKDTNSA KNELLFTRFSTNYNNEPEIFRKGSILCRKEYLS EQILET PTKCSAIRLFHGDVISDKFWTTNPKLLSD
Sbjct:    1 MAKSKFEYVKNFEELDNLLYETWIVCRIDGKNFHSFSTKHNFIKPNDINALMLMNEAAVSVISENTDIFMGYGQSDEFSFIFCSSATIFDRRKSKLLSTIVSKFSSSYVFYWKKYFPNQELLFPPSFDGRIVIYPKAGHVKDYLSWRQADCHINNLYNTCYWKMVLNENKTGKDAENELKDTNSAAKNELLFTRFSTNYNNEPEIFRKGSILCRKEYLSHEQILETKPTKCSAIRLFHGDVISDKFWTTNPKLLSD 256
[Close]
BLAST of tRNA(His) guanylyltransferase vs. Planmine SMEST
Match: SMESG000041162.1 (SMESG000041162.1)

HSP 1 Score: 440.654 bits (1132), Expect = 1.531e-156
Identity = 212/216 (98.15%), Postives = 214/216 (99.07%), Query Frame = 1 
Query:   85 MAKSKFEYVKNFEELDNLLFETWIVCRIDGKNFHSFSTKHNFIKPNDINALMLMNEAAVSVISENTDIFMGYGQSDEFSFIFRSSATIFDRRKSKLLSTIVSKFSSSYVFYWKKYFPNQELLFPPSFDGRIVIYPKAGHVKDYLSWRQADCHINNLYNTCYWKLVLNENKTGKDAENELKDTNSATKNELLFTRFSTNYNNEPEIFRKGSILCRKE 732
            MAKSKFEYVKNFEELDNLL+ETWIVCRIDGKNFHSFSTKHNFIKPNDINALMLMNEAAVSVISENTDIFMGYGQSDEFSFIF SSATIFDRRKSKLLSTIVSKFSSSYVFYWKKYFPNQELLFPPSFDGRIVIYPKAGHVKDYLSWRQADCHINNLYNTCYWK+VLNENKTGKDAENELKDTNSA KNELLFTRFSTNYNNEPEIFRKGSILCRKE
Sbjct:    1 MAKSKFEYVKNFEELDNLLYETWIVCRIDGKNFHSFSTKHNFIKPNDINALMLMNEAAVSVISENTDIFMGYGQSDEFSFIFCSSATIFDRRKSKLLSTIVSKFSSSYVFYWKKYFPNQELLFPPSFDGRIVIYPKAGHVKDYLSWRQADCHINNLYNTCYWKMVLNENKTGKDAENELKDTNSAAKNELLFTRFSTNYNNEPEIFRKGSILCRKE 216
The following BLAST results are available for this feature:
BLAST of tRNA(His) guanylyltransferase vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 2
Match NameE-valueIdentityDescription
THG1L2.952e-9049.06tRNA-histidine guanylyltransferase 1 like [Source:... [more]
THG1L6.669e-3055.43tRNA-histidine guanylyltransferase 1 like [Source:... [more]
back to top
BLAST of tRNA(His) guanylyltransferase vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of tRNA(His) guanylyltransferase vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2
Match NameE-valueIdentityDescription
THG6.878e-8852.16gene:FBgn0283659 transcript:FBtr0303561[more]
THG6.878e-8852.16gene:FBgn0283659 transcript:FBtr0080727[more]
back to top
BLAST of tRNA(His) guanylyltransferase vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
thg1l2.381e-8851.52tRNA-histidine guanylyltransferase 1-like [Source:... [more]
back to top
BLAST of tRNA(His) guanylyltransferase vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSXETT00000004129.17.392e-9249.81tRNA-histidine guanylyltransferase 1-like [Source:... [more]
ENSXETT00000004233.17.639e-9249.81tRNA-histidine guanylyltransferase 1-like [Source:... [more]
ENSXETT00000004245.18.807e-9249.81tRNA-histidine guanylyltransferase 1-like [Source:... [more]
ENSXETT00000004158.19.280e-9249.81tRNA-histidine guanylyltransferase 1-like [Source:... [more]
ENSXETT00000004339.11.344e-9149.81tRNA-histidine guanylyltransferase 1-like [Source:... [more]
back to top
BLAST of tRNA(His) guanylyltransferase vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Thg1l2.775e-9048.33tRNA-histidine guanylyltransferase 1-like (S. cere... [more]
Thg1l1.360e-7246.15tRNA-histidine guanylyltransferase 1-like (S. cere... [more]
back to top
BLAST of tRNA(His) guanylyltransferase vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q05B50|THG1_BOVIN7.645e-9048.69Probable tRNA(His) guanylyltransferase OS=Bos taur... [more]
sp|Q9NWX6|THG1_HUMAN1.418e-8949.06Probable tRNA(His) guanylyltransferase OS=Homo sap... [more]
sp|Q9CY52|THG1_MOUSE1.941e-8948.33Probable tRNA(His) guanylyltransferase OS=Mus musc... [more]
sp|Q5M965|THG1_RAT2.464e-8949.06Probable tRNA(His) guanylyltransferase OS=Rattus n... [more]
sp|Q9V3N8|THG1_DROME6.895e-8752.16Probable tRNA(His) guanylyltransferase OS=Drosophi... [more]
back to top
BLAST of tRNA(His) guanylyltransferase vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A397G9M95.033e-9857.03tRNA(His) guanylyltransferase OS=Diversispora epig... [more]
C3Y4922.617e-9451.87tRNA(His) guanylyltransferase OS=Branchiostoma flo... [more]
A0A3M6TSQ61.218e-9354.33tRNA(His) guanylyltransferase OS=Pocillopora damic... [more]
A0A4X2K1V21.116e-9251.69tRNA(His) guanylyltransferase OS=Vombatus ursinus ... [more]
G3Q3D54.013e-9252.63tRNA(His) guanylyltransferase OS=Gasterosteus acul... [more]
back to top
BLAST of tRNA(His) guanylyltransferase vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
thg1l6.684e-8649.25tRNA-histidine guanylyltransferase 1 like [Source:... [more]
back to top
BLAST of tRNA(His) guanylyltransferase vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 2
Match NameE-valueIdentityDescription
thg1l1.747e-7049.50tRNA-histidine guanylyltransferase 1-like [Source:... [more]
thg1l1.619e-6850.79tRNA-histidine guanylyltransferase 1-like [Source:... [more]
back to top
BLAST of tRNA(His) guanylyltransferase vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1
Match NameE-valueIdentityDescription
THG12.707e-6750.46tRNAHis guanylyltransferase; adds a guanosine resi... [more]
back to top
BLAST of tRNA(His) guanylyltransferase vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 2
Match NameE-valueIdentityDescription
EDO479522.439e-9151.16Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO283965.236e-1728.87Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
back to top
BLAST of tRNA(His) guanylyltransferase vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
thg1l7.567e-8951.12tRNA-histidine guanylyltransferase 1 like [Source:... [more]
back to top
BLAST of tRNA(His) guanylyltransferase vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000041162.10.000e+097.66SMESG000041162.1[more]
SMESG000041162.11.531e-15698.15SMESG000041162.1[more]
back to top
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30028269 ID=SMED30028269|Name=tRNA(His) guanylyltransferase|organism=Schmidtea mediterranea sexual|type=transcript|length=1227bp
TAAAATTTTAAATTTCTTTTCCAAATAATCATGAACTGAATAGTTTGTTT
TAATAATAACTATTTACATCACCGCATTTATTAAATGGCAAAAAGCAAAT
TTGAATATGTTAAAAATTTTGAAGAACTTGACAATCTGTTATTTGAGACT
TGGATAGTATGCAGAATAGATGGTAAAAATTTCCATTCATTTTCAACAAA
ACATAATTTTATCAAACCAAACGATATAAATGCTTTGATGTTAATGAATG
AAGCAGCAGTTTCGGTTATTTCTGAAAACACCGATATTTTTATGGGATAT
GGGCAATCTGACGAGTTTAGTTTCATTTTTCGTAGCTCAGCAACTATTTT
TGATAGACGTAAAAGCAAATTACTATCGACAATAGTTTCAAAATTTTCTA
GCAGTTACGTGTTTTACTGGAAAAAATATTTCCCAAATCAAGAATTACTC
TTTCCTCCTTCATTTGATGGGCGTATCGTTATATATCCCAAAGCTGGTCA
TGTTAAAGATTATTTAAGCTGGCGTCAAGCTGATTGTCATATAAATAATT
TGTATAATACATGTTATTGGAAATTGGTCTTAAATGAAAATAAAACTGGA
AAAGATGCAGAAAATGAATTGAAAGACACAAATTCCGCTACAAAGAATGA
GCTTTTATTCACTCGGTTTAGCACAAATTATAACAATGAACCAGAAATAT
TTAGAAAGGGATCTATTTTATGTCGTAAAGAGTATTTAAGTCGTGAACAA
ATACTGGAAACGAATCCTACAAAATGTTCAGCGATACGATTGTTCCATGG
AGATGTAATCAGTGATAAATTCTGGACAACAAACCCGAAGTTGCTATCGG
ATTAAAACTTCATTATTTTATTCCAATGAAGTTTTTATCGTGAAAGGATT
TAATAGTTAATAAATTTTCGTTCGTTCTGATGTATCACAATAATAATTAA
TTTTCAAAAAAATGGAAAAAGGTGAAATTGAAATCTGTTACGAAGTCGCG
ATATTATCTATGTAACCTGAAATGAAATTGGAGGAGAGCATTATTGCTGG
CTCACAGTTTAGACCAAATCCTAACCGAGTTAGACAATAATATATATACA
GTCTACCCTGTCAATAATGGATACGACGGTAACCTTGTTTTGGGTGTCCA
ATACGGAAAAATGGTGGAAATATAATTTTTTATGAACCTTGTGCTAATAA
TTACATAATGATTTTGTTGGCTACAAA
back to top

protein sequence of SMED30028269-orf-1

>SMED30028269-orf-1 ID=SMED30028269-orf-1|Name=SMED30028269-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=257bp
MAKSKFEYVKNFEELDNLLFETWIVCRIDGKNFHSFSTKHNFIKPNDINA
LMLMNEAAVSVISENTDIFMGYGQSDEFSFIFRSSATIFDRRKSKLLSTI
VSKFSSSYVFYWKKYFPNQELLFPPSFDGRIVIYPKAGHVKDYLSWRQAD
CHINNLYNTCYWKLVLNENKTGKDAENELKDTNSATKNELLFTRFSTNYN
NEPEIFRKGSILCRKEYLSREQILETNPTKCSAIRLFHGDVISDKFWTTN
PKLLSD*
back to top
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: biological process
TermDefinition
GO:0099116tRNA 5'-end processing
GO:0006400tRNA modification
GO:0008033tRNA processing
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002109X1 cell
Vocabulary: INTERPRO
TermDefinition
IPR038469tRNAHis_GuaTrfase_Thg1_sf
IPR024956tRNAHis_GuaTrfase_cat
IPR025845Thg1_C_dom
IPR007537tRNAHis_GuaTrfase_Thg1
Vocabulary: molecular function
TermDefinition
GO:0000287magnesium ion binding
GO:0008193tRNA guanylyltransferase activity
GO:0000166nucleotide binding
GO:0005525GTP binding
GO:0016740transferase activity
GO:0016779nucleotidyltransferase activity
GO:0046872metal ion binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007537tRNAHis guanylyltransferase Thg1PIRSFPIRSF028980tRNAHis_guanlltrcoord: 1..256
e-value: 1.1E-107
score: 357.0
IPR007537tRNAHis guanylyltransferase Thg1PANTHERPTHR12729UNCHARACTERIZEDcoord: 1..255
IPR025845Thg1 C-terminal domainPFAMPF14413Thg1Ccoord: 139..222
e-value: 1.2E-39
score: 135.3
IPR038469tRNAHis guanylyltransferase Thg1 superfamilyGENE3DG3DSA:3.30.70.3000coord: 1..256
e-value: 3.4E-107
score: 359.6
IPR024956tRNAHis guanylyltransferase catalytic domainPFAMPF04446Thg1coord: 6..135
e-value: 1.2E-46
score: 157.8
NoneNo IPR availablePANTHERPTHR12729:SF6TRNA(HIS) GUANYLYLTRANSFERASE-RELATEDcoord: 1..255