AlkB homolog 3, alpha-ketoglutaratedependent dioxygenase

Overview
NameAlkB homolog 3, alpha-ketoglutaratedependent dioxygenase
Smed IDSMED30028242
Length (bp)1596
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of AlkB homolog 3, alpha-ketoglutaratedependent dioxygenase (SMED30028242) t-SNE clustered cells

Violin plots show distribution of expression levels for AlkB homolog 3, alpha-ketoglutaratedependent dioxygenase (SMED30028242) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of AlkB homolog 3, alpha-ketoglutaratedependent dioxygenase (SMED30028242) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for AlkB homolog 3, alpha-ketoglutaratedependent dioxygenase (SMED30028242) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30028242

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30028242h1SMcG0002509 Contig2329GPL15192PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
posterior sideSMED30028242h1SMcG0002509 dd_Smed_v4_7441_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
posterior muscle cellSMED30028242h1SMcG0002509 dd_Smed_v4_7441_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of AlkB homolog 3, alpha-ketoglutaratedependent dioxygenase vs. Ensembl Human
Match: ALKBH3 (alkB homolog 3, alpha-ketoglutaratedependent dioxygenase [Source:HGNC Symbol;Acc:HGNC:30141])

HSP 1 Score: 130.954 bits (328), Expect = 8.064e-34
Identity = 87/290 (30.00%), Postives = 138/290 (47.59%), Query Frame = 3 
Query:   75 VKDKRKANRIQGGWNKNSDSKATCK--IVFEPELKSNSIDIIPTFDDRVIGED---KEKFNIIEVSENISIDINKDSLKYFTNNFPLKHYQILVDETIPATIELEKGFLSSDEASNLCQYFLHHLPWEQRENATPNGEKFLEPRKTCWYG-LPYSYSKIKMPPNDKWDPKLRQLLNQVQKYNNGKPFNSVMCNLYFDNKNSIDWHADDEKSLGENPTIASISLGCSRAFEISYRKDLYVDYKNKSKNPYRSRVKDDPPFDGCSILY----RVFLGEGSLLIMSGSLQNHW 914
            +++KR+  R+QG W     S+A  +     +  L           +  +   +   KE   ++  +    + I+++ +           Y+I +  T  + + L  GF+   EA  + +     +PW+QR     +   + +PR T WYG LPY+YS+I M PN  W P LR L N++++ N G  FNS++CNLY + K+S+DWH+DDE SLG  P IAS+S G +R FE+                      K  PP +     Y    ++ L  G+LLIM G+ Q  W
Sbjct:    1 MEEKRRRARVQGAWAAPVKSQAIAQPATTAKSHLHQKPGQTWKNKEHHLSDREFVFKEPQQVVRRAPEPRV-IDREGV-----------YEISLSPTGVSRVCLYPGFVDVKEADWILEQLCQDVPWKQRTGIREDI-TYQQPRLTAWYGELPYTYSRITMEPNPHWHPVLRTLKNRIEE-NTGHTFNSLLCNLYRNEKDSVDWHSDDEPSLGRCPIIASLSFGATRTFEMR---------------------KKPPPEENGDYTYVERVKIPLDHGTLLIMEGATQADW 255
[Close]
BLAST of AlkB homolog 3, alpha-ketoglutaratedependent dioxygenase vs. Ensembl Human
Match: ALKBH3 (alkB homolog 3, alpha-ketoglutaratedependent dioxygenase [Source:HGNC Symbol;Acc:HGNC:30141])

HSP 1 Score: 122.094 bits (305), Expect = 3.003e-31
Identity = 76/229 (33.19%), Postives = 121/229 (52.84%), Query Frame = 3 
Query:   75 VKDKRKANRIQGGWNKNSDSKATCKIVFEPELKSNS-IDIIP--TFDDRVIGEDKEKFNIIEVSENISIDINKDSLKYFTNNFPLKHYQILVDETIPATIELEKGFLSSDEASNLCQYFLHHLPWEQRENATPNGEKFLEPRKTCWYG-LPYSYSKIKMPPNDKWDPKLRQLLNQVQKYNNGKPFNSVMCNLYFDNKNSIDWHADDEKSLGENPTIASISLGCSRAFEI 749
            +++KR+  R+QG W     S+A    + +P   + S +   P  T+ ++       +F   E  + +        ++          Y+I +  T  + + L  GF+   EA  + +     +PW+QR     +   + +PR T WYG LPY+YS+I M PN  W P LR L N++++ N G  FNS++CNLY + K+S+DWH+DDE SLG  P IAS+S G +R FE+
Sbjct:    1 MEEKRRRARVQGAWAAPVKSQA----IAQPATTAKSHLHQKPGQTWKNKEHHLSDREFVFKEPQQVVRRAPEPRVIEEGV-------YEISLSPTGVSRVCLYPGFVDVKEADWILEQLCQDVPWKQRTGIREDI-TYQQPRLTAWYGELPYTYSRITMEPNPHWHPVLRTLKNRIEE-NTGHTFNSLLCNLYRNEKDSVDWHSDDEPSLGRCPIIASLSFGATRTFEM 216
[Close]
BLAST of AlkB homolog 3, alpha-ketoglutaratedependent dioxygenase vs. Ensembl Human
Match: ALKBH3 (alkB homolog 3, alpha-ketoglutaratedependent dioxygenase [Source:HGNC Symbol;Acc:HGNC:30141])

HSP 1 Score: 88.1965 bits (217), Expect = 6.617e-20
Identity = 53/143 (37.06%), Postives = 75/143 (52.45%), Query Frame = 3 
Query:  516 YSYSKIKMPP------NDKWDPKLRQLLNQVQKYNNGKPFNSVMCNLYFDNKNSIDWHADDEKSLGENPTIASISLGCSRAFEISYRKDLYVDYKNKSKNPYRSRVKDDPPFDGCSILY----RVFLGEGSLLIMSGSLQNHW 914
            Y  S +KM P      +++W P LR L N++++ N G  FNS++CNLY + K+S+DWH+DDE SLG  P IAS+S G +R FE+                      K  PP +     Y    ++ L  G+LLIM G+ Q  W
Sbjct:    5 YWNSFVKMFPGNRGLASERWHPVLRTLKNRIEE-NTGHTFNSLLCNLYRNEKDSVDWHSDDEPSLGRCPIIASLSFGATRTFEMR---------------------KKPPPEENGDYTYVERVKIPLDHGTLLIMEGATQADW 125
[Close]
BLAST of AlkB homolog 3, alpha-ketoglutaratedependent dioxygenase vs. Ensembl Human
Match: ALKBH2 (alkB homolog 2, alpha-ketoglutarate dependent dioxygenase [Source:HGNC Symbol;Acc:HGNC:32487])

HSP 1 Score: 65.0846 bits (157), Expect = 3.840e-11
Identity = 50/165 (30.30%), Postives = 73/165 (44.24%), Query Frame = 3 
Query:  486 PRKTCWYG---LPYSYSKIKMPPNDKWDPKLRQLLNQVQKYNNGKPFNSVMCNLYFDNKNSIDWHADDEKSLGENPTIASISLGCSRAFEISYRKDLYVDYKNKSKNPYRSRVKDDPPFDGCSILYRVFLGEGSLLIMSGSLQNHWLVGFISINIYSTFPYKNKT 971
            PRK   YG   L Y++S + + P   W P L ++ + V     G+ FN V+ N Y D  + I  H DDE+ L     IAS+S G  R F   ++        ++ K+P R RV           + R+ L  GSLL+M+     HW         Y + P + K 
Sbjct:  109 PRKQATYGDAGLTYTFSGLTLSPKP-WIPVLERIRDHVSGVT-GQTFNFVLINRYKDGCDHIGEHRDDERELAPGSPIASVSFGACRDFVFRHK-------DSRGKSPSR-RV----------AVVRLPLAHGSLLMMNHPTNTHW---------YHSLPVRKKV 244
[Close]
BLAST of AlkB homolog 3, alpha-ketoglutaratedependent dioxygenase vs. Ensembl Human
Match: ALKBH2 (alkB homolog 2, alpha-ketoglutarate dependent dioxygenase [Source:HGNC Symbol;Acc:HGNC:32487])

HSP 1 Score: 65.0846 bits (157), Expect = 3.840e-11
Identity = 50/165 (30.30%), Postives = 73/165 (44.24%), Query Frame = 3 
Query:  486 PRKTCWYG---LPYSYSKIKMPPNDKWDPKLRQLLNQVQKYNNGKPFNSVMCNLYFDNKNSIDWHADDEKSLGENPTIASISLGCSRAFEISYRKDLYVDYKNKSKNPYRSRVKDDPPFDGCSILYRVFLGEGSLLIMSGSLQNHWLVGFISINIYSTFPYKNKT 971
            PRK   YG   L Y++S + + P   W P L ++ + V     G+ FN V+ N Y D  + I  H DDE+ L     IAS+S G  R F   ++        ++ K+P R RV           + R+ L  GSLL+M+     HW         Y + P + K 
Sbjct:  109 PRKQATYGDAGLTYTFSGLTLSPKP-WIPVLERIRDHVSGVT-GQTFNFVLINRYKDGCDHIGEHRDDERELAPGSPIASVSFGACRDFVFRHK-------DSRGKSPSR-RV----------AVVRLPLAHGSLLMMNHPTNTHW---------YHSLPVRKKV 244
[Close]
BLAST of AlkB homolog 3, alpha-ketoglutaratedependent dioxygenase vs. Ensembl Zebrafish
Match: alkbh3 (alkB homolog 3, alpha-ketoglutarate-dependent dioxygenase [Source:ZFIN;Acc:ZDB-GENE-040801-254])

HSP 1 Score: 127.487 bits (319), Expect = 8.780e-33
Identity = 93/283 (32.86%), Postives = 130/283 (45.94%), Query Frame = 3 
Query:   75 VKDKRKANRIQGGWNK--NSDSKATCKIVFEPELKSNSIDIIPTFDDRVIGEDKEKFNIIEVSENISIDINKDSLKYFTNNFPLKHYQILVDETIPATIELEKGFLSSDEASNLCQYFLHHLPWEQRENATPNGEKFLEPRKTCWYG-LPYSYSKIKMPPNDKWDPKLRQLLNQVQKYNNGKPFNSVMCNLYFDNKNSIDWHADDEKSLGENPTIASISLGCSRAFEISYRKDLYVDYKNKSKNPYRSRVKDDPPFDGCSILYRVFLGEGSLLIMSGSLQNHW 914
            + DKR+  R+QG W K      + + + V       +      +F+     +   K    +V EN                     Y+I    +  + + L  GFL  +EA  +    L  LPW Q+ N    G+ + EPR TCWYG LPY+YS+  M  N +W P L  L   V++ +  K FNS++CNLY D K+SI WH+D E SLG  P IAS+SLG +R F  S RK    +  +K    Y  R+             RV L  G+LL+M G  Q  W
Sbjct:    1 MSDKRQRARVQGSWAKPVQKPQRPSVQDVQSASASGSWKSGPQSFEFHQPADPIRKVPAEKVIENAG------------------DYEISQGPSGVSRLRLIPGFLLQEEADWMFSKLLAELPWSQKTNYRMMGDAYEEPRLTCWYGELPYTYSRSTMEANAQWHPVLATLRLAVEQKSAHK-FNSLLCNLYRDGKDSIGWHSDSEPSLGPQPIIASLSLGDTRVF--SLRKQPLPE--DKGDFTYVERI-------------RVPLAHGTLLLMEGCTQADW 247
[Close]
BLAST of AlkB homolog 3, alpha-ketoglutaratedependent dioxygenase vs. Ensembl Zebrafish
Match: alkbh3 (alkB homolog 3, alpha-ketoglutarate-dependent dioxygenase [Source:ZFIN;Acc:ZDB-GENE-040801-254])

HSP 1 Score: 127.487 bits (319), Expect = 8.780e-33
Identity = 93/283 (32.86%), Postives = 130/283 (45.94%), Query Frame = 3 
Query:   75 VKDKRKANRIQGGWNK--NSDSKATCKIVFEPELKSNSIDIIPTFDDRVIGEDKEKFNIIEVSENISIDINKDSLKYFTNNFPLKHYQILVDETIPATIELEKGFLSSDEASNLCQYFLHHLPWEQRENATPNGEKFLEPRKTCWYG-LPYSYSKIKMPPNDKWDPKLRQLLNQVQKYNNGKPFNSVMCNLYFDNKNSIDWHADDEKSLGENPTIASISLGCSRAFEISYRKDLYVDYKNKSKNPYRSRVKDDPPFDGCSILYRVFLGEGSLLIMSGSLQNHW 914
            + DKR+  R+QG W K      + + + V       +      +F+     +   K    +V EN                     Y+I    +  + + L  GFL  +EA  +    L  LPW Q+ N    G+ + EPR TCWYG LPY+YS+  M  N +W P L  L   V++ +  K FNS++CNLY D K+SI WH+D E SLG  P IAS+SLG +R F  S RK    +  +K    Y  R+             RV L  G+LL+M G  Q  W
Sbjct:    1 MSDKRQRARVQGSWAKPVQKPQRPSVQDVQSASASGSWKSGPQSFEFHQPADPIRKVPAEKVIENAG------------------DYEISQGPSGVSRLRLIPGFLLQEEADWMFSKLLAELPWSQKTNYRMMGDAYEEPRLTCWYGELPYTYSRSTMEANAQWHPVLATLRLAVEQKSAHK-FNSLLCNLYRDGKDSIGWHSDSEPSLGPQPIIASLSLGDTRVF--SLRKQPLPE--DKGDFTYVERI-------------RVPLAHGTLLLMEGCTQADW 247
[Close]
BLAST of AlkB homolog 3, alpha-ketoglutaratedependent dioxygenase vs. Ensembl Zebrafish
Match: alkbh3 (alkB homolog 3, alpha-ketoglutarate-dependent dioxygenase [Source:ZFIN;Acc:ZDB-GENE-040801-254])

HSP 1 Score: 127.487 bits (319), Expect = 8.780e-33
Identity = 93/283 (32.86%), Postives = 130/283 (45.94%), Query Frame = 3 
Query:   75 VKDKRKANRIQGGWNK--NSDSKATCKIVFEPELKSNSIDIIPTFDDRVIGEDKEKFNIIEVSENISIDINKDSLKYFTNNFPLKHYQILVDETIPATIELEKGFLSSDEASNLCQYFLHHLPWEQRENATPNGEKFLEPRKTCWYG-LPYSYSKIKMPPNDKWDPKLRQLLNQVQKYNNGKPFNSVMCNLYFDNKNSIDWHADDEKSLGENPTIASISLGCSRAFEISYRKDLYVDYKNKSKNPYRSRVKDDPPFDGCSILYRVFLGEGSLLIMSGSLQNHW 914
            + DKR+  R+QG W K      + + + V       +      +F+     +   K    +V EN                     Y+I    +  + + L  GFL  +EA  +    L  LPW Q+ N    G+ + EPR TCWYG LPY+YS+  M  N +W P L  L   V++ +  K FNS++CNLY D K+SI WH+D E SLG  P IAS+SLG +R F  S RK    +  +K    Y  R+             RV L  G+LL+M G  Q  W
Sbjct:    1 MSDKRQRARVQGSWAKPVQKPQRPSVQDVQSASASGSWKSGPQSFEFHQPADPIRKVPAEKVIENAG------------------DYEISQGPSGVSRLRLIPGFLLQEEADWMFSKLLAELPWSQKTNYRMMGDAYEEPRLTCWYGELPYTYSRSTMEANAQWHPVLATLRLAVEQKSAHK-FNSLLCNLYRDGKDSIGWHSDSEPSLGPQPIIASLSLGDTRVF--SLRKQPLPE--DKGDFTYVERI-------------RVPLAHGTLLLMEGCTQADW 247
[Close]
BLAST of AlkB homolog 3, alpha-ketoglutaratedependent dioxygenase vs. Ensembl Zebrafish
Match: alkbh3 (alkB homolog 3, alpha-ketoglutarate-dependent dioxygenase [Source:ZFIN;Acc:ZDB-GENE-040801-254])

HSP 1 Score: 85.1149 bits (209), Expect = 9.307e-19
Identity = 62/197 (31.47%), Postives = 93/197 (47.21%), Query Frame = 3 
Query:   75 VKDKRKANRIQGGWNKNSDSKATCKIVFEPELKSNSIDIIPTFDDRVIGEDKEKFNIIEVSENISIDINKDSLKYFTNNFPLKHYQILVDETIPATIELEKGFLSSDEASNLCQYFLHHLPWEQRENATPNGEKFLEPRKTCWYG-LPYSYSKIKMPPNDKWDPKLRQLLNQVQKYNNGKPFNSVMCNLYFDNKNSI 662
            + DKR+  R+QG W          K V +P+ + +  D+             + F   + ++     I K   +    N     Y+I    +  + + L  GFL  +EA  +    L  LPW Q+ N    G+ + EPR TCWYG LPY+YS+  M  N +W P L  L   V++ +  K FNS++CNLY D K+SI
Sbjct:    1 MSDKRQRARVQGSW---------AKPVQKPQ-RPSVQDVQSASASGSWKSGPQSFEFHQPAD----PIRKVPAEKVIEN--AGDYEISQGPSGVSRLRLIPGFLLQEEADWMFSKLLAELPWSQKTNYRMMGDAYEEPRLTCWYGELPYTYSRSTMEANAQWHPVLATLRLAVEQKSAHK-FNSLLCNLYRDGKDSI 180
[Close]
BLAST of AlkB homolog 3, alpha-ketoglutaratedependent dioxygenase vs. Ensembl Zebrafish
Match: alkbh2 (alkB homolog 2, alpha-ketoglutarate-dependent dioxygenase [Source:ZFIN;Acc:ZDB-GENE-060526-327])

HSP 1 Score: 53.5286 bits (127), Expect = 1.201e-7
Identity = 43/152 (28.29%), Postives = 67/152 (44.08%), Query Frame = 3 
Query:  468 GEKFLEPRKTCWYG---LPYSYSKIKMPPNDKWDPKLRQLLNQVQKYNNGKPFNSVMCNLYFDNKNSIDWHADDEKSLGENPTIASISLGCSRAFEISYRKDLYVDYKNKSKNPYRSRVKDDPPFDGCSILYRVFLGEGSLLIMSGSLQNHW 914
            G+ +  PRK   YG   L YS+S + +     W   L  + + V K   G  FN V+ N Y D  + I  H DDE+ L     IAS+SLG +R         L   +++    P + +++            ++ L  GSLL+M+     +W
Sbjct:   61 GKSYNIPRKQATYGDEGLMYSFSGVNLLAK-PWTSTLEHIRDAVTKAT-GYTFNFVLINRYKDGHDHIGEHRDDERELDPACPIASVSLGAAR--------HLIFRHRDARTGPRKRQIEP----------VKLELANGSLLLMNFPTNTYW 192
[Close]
BLAST of AlkB homolog 3, alpha-ketoglutaratedependent dioxygenase vs. Ensembl Xenopus
Match: ALKBH3 (alkB homolog 3, alpha-ketoglutarate-dependent dioxygenase [Source:NCBI gene;Acc:100495476])

HSP 1 Score: 121.709 bits (304), Expect = 1.212e-30
Identity = 82/285 (28.77%), Postives = 130/285 (45.61%), Query Frame = 3 
Query:   75 VKDKRKANRIQGGWNKNSDSKATCKIVFEPELKSNSIDIIPTFDDRVIGEDKEKFNIIEVSENISIDINKDSLKYFTNNFPLKHYQILVDETIPATIELEKGFLSSDEASNLCQYFLHHLPWEQRENATPNGEKFLEPRKTCWYG-LPYSYSKIKMPPNDKWDPKLRQLLNQVQKYNNGKPFNSVMCNLYFDNKNSIDWHADDEKSLGENPTIASISLGCSRAFEISYRKDLYVDYKNKSKNPYRSRVKDDPPFDGCSILY----RVFLGEGSLLIMSGSLQNHW 914
            + +KR+  R+QGGW     ++   +    P  +  +        ++     +    +  V E   ID                 ++I    +  + + L + F+   EA  + +     +PW Q+ N  P+G  + EPR TCWYG LPY+YS+  M  N  W P L  L +++++   G  FNS++CNLY  +K+SIDWH+DDE +LG +P IAS+S G +R F++                      K  PP +     Y     V L  G+LL+M G+ Q  W
Sbjct:    1 MDNKRQRARVQGGWA--GGARPGSRPAVPPAQRPPAWGKEQPTQEKHFVYSEPAEAVRRVPEPQVIDAAGT-------------HEISFSPSGVSRLRLFRSFIDPREADWMFKQLHQEIPWSQKTNVGPDG-SYQEPRLTCWYGELPYTYSRSMMQANPHWHPLLTMLKDRIEEVT-GYGFNSLLCNLYRHDKDSIDWHSDDEPALGTSPIIASLSFGETRNFQMR---------------------KKPPPEERGDYTYVERVHVPLDHGTLLLMEGATQQDW 247
[Close]
BLAST of AlkB homolog 3, alpha-ketoglutaratedependent dioxygenase vs. Ensembl Xenopus
Match: ALKBH3 (alkB homolog 3, alpha-ketoglutarate-dependent dioxygenase [Source:NCBI gene;Acc:100495476])

HSP 1 Score: 119.398 bits (298), Expect = 4.574e-28
Identity = 87/286 (30.42%), Postives = 130/286 (45.45%), Query Frame = 3 
Query:   81 DKRKANRIQGGWNKNSDSKATCKIVFEPELKSNSI--DIIPTFDDR-VIGEDKEKFNIIEVSENISIDINKDSLKYFTNNFPLKHYQILVDETIPATIELEKGFLSSDEASNLCQYFLHHLPWEQRENATPNGEKFLEPRKTCWYG-LPYSYSKIKMPPNDKWDPKLRQLLNQVQKYNNGKPFNSVMCNLYFDNKNSIDWHADDEKSLGENPTIASISLGCSRAFEISYRKDLYVDYKNKSKNPYRSRVKDDPPFDGCSILY----RVFLGEGSLLIMSGSLQNHW 914
            +KR+  R+QGGW   +   +       P  +         PT +   V  E  E   +  V E   ID                 ++I    +  + + L + F+   EA  + +     +PW Q+ N  P+G  + EPR TCWYG LPY+YS+  M  N  W P L  L +++++   G  FNS++CNLY  +K+SIDWH+DDE +LG +P IAS+S G +R F++                      K  PP +     Y     V L  G+LL+M G+ Q  W
Sbjct:    3 NKRQRARVQGGWAGGARPGSRPGFPAVPPAQRPPAWGKEQPTQEKHFVYSEPAEA--VRRVPEPQVIDAAGT-------------HEISFSPSGVSRLRLFRSFIDPREADWMFKQLHQEIPWSQKTNVGPDG-SYQEPRLTCWYGELPYTYSRSMMQANPHWHPLLTMLKDRIEEVT-GYGFNSLLCNLYRHDKDSIDWHSDDEPALGTSPIIASLSFGETRNFQMR---------------------KKPPPEERGDYTYVERVHVPLDHGTLLLMEGATQQDW 250
[Close]
BLAST of AlkB homolog 3, alpha-ketoglutaratedependent dioxygenase vs. Ensembl Xenopus
Match: g6pc.1 (glucose-6-phosphatase, catalytic subunit, gene 1 [Source:Xenbase;Acc:XB-GENE-963989])

HSP 1 Score: 58.9214 bits (141), Expect = 4.791e-9
Identity = 54/166 (32.53%), Postives = 70/166 (42.17%), Query Frame = 3 
Query:  486 PRKTCWYG---LPYSYSKIKMPPNDKWDPKLRQLLNQVQKYNNGKPFNSVMCNLYFDNKNSIDWHADDEKSLGENPTIASISLGCSRAFEISYRKDLYVDYKNKSKNPYRSRVKDDPPFDGCSIL-YRVFLGEGSLLIMSGSLQNHWLVGFISINIYSTFPYKNKT 971
            PRK   YG   L Y++S I + P   W P L  +  ++Q    G  FN V+ N Y D  + I  H DDEK L     IAS+S G  R F I   KD       + KNP             C I   +V L  GSLL+M+     +W         Y + P + K 
Sbjct:  127 PRKQVMYGDEGLHYTFSGITLSPK-PWIPVLVHIKERLQ-LATGHSFNFVLINRYKDGNDHIGEHRDDEKELVPQSPIASVSFGACRDF-IFRHKDA------RCKNPM------------CHIQPVKVELAHGSLLMMNYPTNVYW---------YHSLPVRKKV 262
[Close]
BLAST of AlkB homolog 3, alpha-ketoglutaratedependent dioxygenase vs. Ensembl Xenopus
Match: g6pc.1 (glucose-6-phosphatase, catalytic subunit, gene 1 [Source:Xenbase;Acc:XB-GENE-963989])

HSP 1 Score: 57.7658 bits (138), Expect = 1.698e-8
Identity = 54/166 (32.53%), Postives = 70/166 (42.17%), Query Frame = 3 
Query:  486 PRKTCWYG---LPYSYSKIKMPPNDKWDPKLRQLLNQVQKYNNGKPFNSVMCNLYFDNKNSIDWHADDEKSLGENPTIASISLGCSRAFEISYRKDLYVDYKNKSKNPYRSRVKDDPPFDGCSIL-YRVFLGEGSLLIMSGSLQNHWLVGFISINIYSTFPYKNKT 971
            PRK   YG   L Y++S I + P   W P L  +  ++Q    G  FN V+ N Y D  + I  H DDEK L     IAS+S G  R F I   KD       + KNP             C I   +V L  GSLL+M+     +W         Y + P + K 
Sbjct:  192 PRKQVMYGDEGLHYTFSGITLSPK-PWIPVLVHIKERLQ-LATGHSFNFVLINRYKDGNDHIGEHRDDEKELVPQSPIASVSFGACRDF-IFRHKDA------RCKNPM------------CHIQPVKVELAHGSLLMMNYPTNVYW---------YHSLPVRKKV 327
[Close]
BLAST of AlkB homolog 3, alpha-ketoglutaratedependent dioxygenase vs. Ensembl Mouse
Match: Alkbh3 (alkB homolog 3, alpha-ketoglutarate-dependent dioxygenase [Source:MGI Symbol;Acc:MGI:1916363])

HSP 1 Score: 127.102 bits (318), Expect = 1.548e-32
Identity = 88/295 (29.83%), Postives = 140/295 (47.46%), Query Frame = 3 
Query:   75 VKDKRKANRIQGGWNKNSDSKATCKIVFEPELKSNSIDIIPTFDDRVIGEDKEKFN------IIEVSENISIDINKDSLKYFTNNFPLKHYQILVDETIPATIELEKGFLSSDEASNLCQYFLHHLPWEQR----ENATPNGEKFLEPRKTCWYG-LPYSYSKIKMPPNDKWDPKLRQLLNQVQKYNNGKPFNSVMCNLYFDNKNSIDWHADDEKSLGENPTIASISLGCSRAFEISYRKDLYVDYKNKSKNPYRSRVKDDPPFDGCSILY----RVFLGEGSLLIMSGSLQNHW 914
            ++DKR+  R+QGGW   + S++  +    P     S    P +  +   +   +F       ++  +    + I+++ +           Y+I +  T  + + L  GF+   EA  + +     +PW+QR    E+ T     + +PR T WYG LPY+YS+I M PN  W P L  L +++++ N    FNS++CN Y D K+S+DWH+DDE SLG  P IAS+S G +R FE+                      K  PP +     Y    ++ L  G+LLIM G+ Q  W
Sbjct:    1 MEDKRQRARVQGGWATPTKSQSATQPA-SPARSRLSQTAGPAWRSKEQQQCDRQFVFKEPQLVVRAAPEPRV-IDREGV-----------YEISLSPTGVSRVCLYPGFVDLKEADWILEQLCKDVPWKQRMGIREDVT-----YPQPRLTAWYGELPYTYSRITMEPNPHWLPVLWTLKSRIEE-NTSHTFNSLLCNFYRDEKDSVDWHSDDEPSLGSCPVIASLSFGATRTFEMR---------------------KKPPPEENGDYTYVERVKIPLDHGTLLIMEGATQADW 255
[Close]
BLAST of AlkB homolog 3, alpha-ketoglutaratedependent dioxygenase vs. Ensembl Mouse
Match: Alkbh3 (alkB homolog 3, alpha-ketoglutarate-dependent dioxygenase [Source:MGI Symbol;Acc:MGI:1916363])

HSP 1 Score: 125.176 bits (313), Expect = 7.956e-32
Identity = 86/289 (29.76%), Postives = 139/289 (48.10%), Query Frame = 3 
Query:   75 VKDKRKANRIQGGWNKNSDSKATCKIVFEPELKSNSIDIIPTFDDRVIGEDKEKFNIIEVSENISIDINKDSLKYFTNNFPLKHYQILVDETIPATIELEKGFLSSDEASNLCQYFLHHLPWEQR----ENATPNGEKFLEPRKTCWYG-LPYSYSKIKMPPNDKWDPKLRQLLNQVQKYNNGKPFNSVMCNLYFDNKNSIDWHADDEKSLGENPTIASISLGCSRAFEISYRKDLYVDYKNKSKNPYRSRVKDDPPFDGCSILY----RVFLGEGSLLIMSGSLQNHW 914
            ++DKR+  R+QGGW   + S++      +P      + +  T       +++++ +   V +   + +         +   +  Y+I +  T  + + L  GF+   EA  + +     +PW+QR    E+ T     + +PR T WYG LPY+YS+I M PN  W P L  L +++++ N    FNS++CN Y D K+S+DWH+DDE SLG  P IAS+S G +R FE+                      K  PP +     Y    ++ L  G+LLIM G+ Q  W
Sbjct:    1 MEDKRQRARVQGGWATPTKSQSAT----QPGKNLQRLRLSQTAGPAWRSKEQQQCDRQFVFKEPQLVVRAAPEPRVIDREGV--YEISLSPTGVSRVCLYPGFVDLKEADWILEQLCKDVPWKQRMGIREDVT-----YPQPRLTAWYGELPYTYSRITMEPNPHWLPVLWTLKSRIEE-NTSHTFNSLLCNFYRDEKDSVDWHSDDEPSLGSCPVIASLSFGATRTFEMR---------------------KKPPPEENGDYTYVERVKIPLDHGTLLIMEGATQADW 256
[Close]
BLAST of AlkB homolog 3, alpha-ketoglutaratedependent dioxygenase vs. Ensembl Mouse
Match: Alkbh3 (alkB homolog 3, alpha-ketoglutarate-dependent dioxygenase [Source:MGI Symbol;Acc:MGI:1916363])

HSP 1 Score: 97.4413 bits (241), Expect = 6.738e-23
Identity = 64/212 (30.19%), Postives = 109/212 (51.42%), Query Frame = 3 
Query:   75 VKDKRKANRIQGGWNKNSDSKATCKIVFEPELKSNSIDIIPTFDDRVIGEDKEKFNIIEVSENISIDINKDSLKYFTNNFPLKHYQILVDETIPATIELEKGFLSSDEASNLCQYFLHHLPWEQR----ENATPNGEKFLEPRKTCWYG-LPYSYSKIKMPPNDKWDPKLRQLLNQVQKYNNGKPFNSVMCNLYFDNKNSIDWHADDEKSLG 695
            ++DKR+  R+QGGW   + S++      +P      + +  T       +++++ +   V +   + +         +   +  Y+I +  T  + + L  GF+   EA  + +     +PW+QR    E+ T     + +PR T WYG LPY+YS+I M PN  W P L  L +++++ N    FNS++CN Y D K+S+DWH+DDE SLG
Sbjct:    1 MEDKRQRARVQGGWATPTKSQSAT----QPGKNLQRLRLSQTAGPAWRSKEQQQCDRQFVFKEPQLVVRAAPEPRVIDREGV--YEISLSPTGVSRVCLYPGFVDLKEADWILEQLCKDVPWKQRMGIREDVT-----YPQPRLTAWYGELPYTYSRITMEPNPHWLPVLWTLKSRIEE-NTSHTFNSLLCNFYRDEKDSVDWHSDDEPSLG 200
[Close]
BLAST of AlkB homolog 3, alpha-ketoglutaratedependent dioxygenase vs. Ensembl Mouse
Match: Gm27027 (predicted gene, 27027 [Source:MGI Symbol;Acc:MGI:5504142])

HSP 1 Score: 68.5514 bits (166), Expect = 7.968e-14
Identity = 34/76 (44.74%), Postives = 45/76 (59.21%), Query Frame = 3 
Query:  600 NNGKPFNSVMCNLYFDNKNSIDWHADDEKSLGENPTIASISLGCSRAFEISYRKDLYVDYKNKSKNPYRSRVKDDP 827
            N    FNS++CN Y D K+S+DWH+DDE SLG  P IAS+S G +R FE+  RK         S  P+  R+  +P
Sbjct:    3 NTSHTFNSLLCNFYRDEKDSVDWHSDDEPSLGSCPVIASLSFGATRTFEM--RKKPPPRSSTGSWAPWLWRLAGEP 76
[Close]
BLAST of AlkB homolog 3, alpha-ketoglutaratedependent dioxygenase vs. Ensembl Mouse
Match: Alkbh2 (alkB homolog 2, alpha-ketoglutarate-dependent dioxygenase [Source:MGI Symbol;Acc:MGI:2141032])

HSP 1 Score: 64.3142 bits (155), Expect = 4.383e-11
Identity = 48/146 (32.88%), Postives = 69/146 (47.26%), Query Frame = 3 
Query:  486 PRKTCWYG---LPYSYSKIKMPPNDKWDPKLRQLLNQVQKYNNGKPFNSVMCNLYFDNKNSIDWHADDEKSLGENPTIASISLGCSRAFEISYRKDLYVDYKNKSKNPYRSRVKDDPPFDGCSILYRVFLGEGSLLIMSGSLQNHW 914
            PRK   YG   L Y++S + + P   W P L ++ ++V +   G+ FN V+ N Y D  + I  H DDE+ L     IAS+S G  R F I   KD      ++ K P R+             + R+ L  GSLL+M+     HW
Sbjct:   87 PRKQATYGDAGLTYTFSGLTLTPK-PWVPVLERVRDRVCEVT-GQTFNFVLVNRYKDGCDHIGEHRDDERELAPGSPIASVSFGACRDF-IFRHKD------SRGKRPRRT-----------VEVVRLQLAHGSLLMMNPPTNTHW 212
[Close]
BLAST of AlkB homolog 3, alpha-ketoglutaratedependent dioxygenase vs. UniProt/SwissProt
Match: sp|Q32L00|ALKB3_BOVIN (Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3 OS=Bos taurus OX=9913 GN=ALKBH3 PE=2 SV=1)

HSP 1 Score: 136.346 bits (342), Expect = 4.678e-35
Identity = 94/292 (32.19%), Postives = 141/292 (48.29%), Query Frame = 3 
Query:   75 VKDKRKANRIQGGWNKNSDSKATCKIVFEPELKSNSIDIIP--TFDDRVIGEDKEKFNIIEVSENISID-----INKDSLKYFTNNFPLKHYQILVDETIPATIELEKGFLSSDEASNLCQYFLHHLPWEQRENATPNGEKFLEPRKTCWYG-LPYSYSKIKMPPNDKWDPKLRQLLNQVQKYNNGKPFNSVMCNLYFDNKNSIDWHADDEKSLGENPTIASISLGCSRAFEISYRKDLYVDYKNKSKNPYRSRVKDDPPFDGCSILY----RVFLGEGSLLIMSGSLQNHW 914
            ++DKR+  R+QG W   + S+AT +     E   N++   P   + ++       +F   E  E +        I K+ +           Y+I +  T  + + L  GF+   EA ++ +     +PW+QR     +   + +PR T WYG LPY+YS+I M PN  W P L  L NQ+++ N G  FNS++CNLY + K+S+DWH+DDE SLG  P IAS+S G +R FE+                      K  PP D     Y    ++ L  G+LLIM G+ Q  W
Sbjct:    1 MEDKRRRARVQGAWAGPAKSQATAQPAPTAE---NNLQQRPGKAWMNKEQHLSDRQFVFKEPQEGVRRAPEPRVIEKEGV-----------YEISMSPTGISRVCLCPGFVDLKEADSVFEQLCRDVPWKQR-TGIRDDVTYQQPRLTAWYGELPYTYSRITMEPNPHWHPVLLMLKNQIEE-NTGHSFNSLLCNLYRNEKDSVDWHSDDEPSLGRCPIIASLSFGATRMFEMR---------------------KKPPPEDNGDYTYVERVKIPLDHGTLLIMEGATQADW 255
[Close]
BLAST of AlkB homolog 3, alpha-ketoglutaratedependent dioxygenase vs. UniProt/SwissProt
Match: sp|Q96Q83|ALKB3_HUMAN (Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3 OS=Homo sapiens OX=9606 GN=ALKBH3 PE=1 SV=1)

HSP 1 Score: 130.954 bits (328), Expect = 3.873e-33
Identity = 87/290 (30.00%), Postives = 138/290 (47.59%), Query Frame = 3 
Query:   75 VKDKRKANRIQGGWNKNSDSKATCK--IVFEPELKSNSIDIIPTFDDRVIGED---KEKFNIIEVSENISIDINKDSLKYFTNNFPLKHYQILVDETIPATIELEKGFLSSDEASNLCQYFLHHLPWEQRENATPNGEKFLEPRKTCWYG-LPYSYSKIKMPPNDKWDPKLRQLLNQVQKYNNGKPFNSVMCNLYFDNKNSIDWHADDEKSLGENPTIASISLGCSRAFEISYRKDLYVDYKNKSKNPYRSRVKDDPPFDGCSILY----RVFLGEGSLLIMSGSLQNHW 914
            +++KR+  R+QG W     S+A  +     +  L           +  +   +   KE   ++  +    + I+++ +           Y+I +  T  + + L  GF+   EA  + +     +PW+QR     +   + +PR T WYG LPY+YS+I M PN  W P LR L N++++ N G  FNS++CNLY + K+S+DWH+DDE SLG  P IAS+S G +R FE+                      K  PP +     Y    ++ L  G+LLIM G+ Q  W
Sbjct:    1 MEEKRRRARVQGAWAAPVKSQAIAQPATTAKSHLHQKPGQTWKNKEHHLSDREFVFKEPQQVVRRAPEPRV-IDREGV-----------YEISLSPTGVSRVCLYPGFVDVKEADWILEQLCQDVPWKQRTGIREDI-TYQQPRLTAWYGELPYTYSRITMEPNPHWHPVLRTLKNRIEE-NTGHTFNSLLCNLYRNEKDSVDWHSDDEPSLGRCPIIASLSFGATRTFEMR---------------------KKPPPEENGDYTYVERVKIPLDHGTLLIMEGATQADW 255
[Close]
BLAST of AlkB homolog 3, alpha-ketoglutaratedependent dioxygenase vs. UniProt/SwissProt
Match: sp|Q5XIC8|ALKB3_RAT (Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3 OS=Rattus norvegicus OX=10116 GN=Alkbh3 PE=2 SV=1)

HSP 1 Score: 127.872 bits (320), Expect = 6.094e-32
Identity = 87/293 (29.69%), Postives = 139/293 (47.44%), Query Frame = 3 
Query:   81 DKRKANRIQGGWNKNSDSKATCKIVFEPELKSNSIDIIPTFDDRVIGEDKEKFN------IIEVSENISIDINKDSLKYFTNNFPLKHYQILVDETIPATIELEKGFLSSDEASNLCQYFLHHLPWEQR----ENATPNGEKFLEPRKTCWYG-LPYSYSKIKMPPNDKWDPKLRQLLNQVQKYNNGKPFNSVMCNLYFDNKNSIDWHADDEKSLGENPTIASISLGCSRAFEISYRKDLYVDYKNKSKNPYRSRVKDDPPFDGCSILY----RVFLGEGSLLIMSGSLQNHW 914
            DKR+  R+QG W   + S++  +    P     S    P++  +   +   +F       ++  +    + I+++ +           Y+I +  T  + + L  GF+   EA  + +     +PW+QR    E+ T     + +PR T WYG LPY+YS++ M PN  W P L  L +++++ N G  FNS++CN Y D K+S+DWH+DDE SLG  P IAS+S G +R FE+                      K  PP +     Y    ++ L  G+LLIM G+ Q  W
Sbjct:    3 DKRQRARVQGAWATPTKSQSAARPA-TPARSRPSQTPGPSWRSKEQQQCDRRFVFKEPQLVVRAAPEPRV-IDREGV-----------YEISLSPTGVSRVCLYPGFVDLKEADWILERLCQDVPWKQRMGIREDIT-----YPQPRLTAWYGELPYTYSRVTMEPNPHWLPVLWTLKSRIEE-NTGHTFNSLLCNFYRDEKDSVDWHSDDEPSLGSCPVIASLSFGATRTFEMR---------------------KKPPPEENGDYTYVERVKIPLDHGTLLIMEGATQADW 255
[Close]
BLAST of AlkB homolog 3, alpha-ketoglutaratedependent dioxygenase vs. UniProt/SwissProt
Match: sp|Q8K1E6|ALKB3_MOUSE (Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3 OS=Mus musculus OX=10090 GN=Alkbh3 PE=1 SV=1)

HSP 1 Score: 127.102 bits (318), Expect = 1.083e-31
Identity = 88/295 (29.83%), Postives = 140/295 (47.46%), Query Frame = 3 
Query:   75 VKDKRKANRIQGGWNKNSDSKATCKIVFEPELKSNSIDIIPTFDDRVIGEDKEKFN------IIEVSENISIDINKDSLKYFTNNFPLKHYQILVDETIPATIELEKGFLSSDEASNLCQYFLHHLPWEQR----ENATPNGEKFLEPRKTCWYG-LPYSYSKIKMPPNDKWDPKLRQLLNQVQKYNNGKPFNSVMCNLYFDNKNSIDWHADDEKSLGENPTIASISLGCSRAFEISYRKDLYVDYKNKSKNPYRSRVKDDPPFDGCSILY----RVFLGEGSLLIMSGSLQNHW 914
            ++DKR+  R+QGGW   + S++  +    P     S    P +  +   +   +F       ++  +    + I+++ +           Y+I +  T  + + L  GF+   EA  + +     +PW+QR    E+ T     + +PR T WYG LPY+YS+I M PN  W P L  L +++++ N    FNS++CN Y D K+S+DWH+DDE SLG  P IAS+S G +R FE+                      K  PP +     Y    ++ L  G+LLIM G+ Q  W
Sbjct:    1 MEDKRQRARVQGGWATPTKSQSATQPA-SPARSRLSQTAGPAWRSKEQQQCDRQFVFKEPQLVVRAAPEPRV-IDREGV-----------YEISLSPTGVSRVCLYPGFVDLKEADWILEQLCKDVPWKQRMGIREDVT-----YPQPRLTAWYGELPYTYSRITMEPNPHWLPVLWTLKSRIEE-NTSHTFNSLLCNFYRDEKDSVDWHSDDEPSLGSCPVIASLSFGATRTFEMR---------------------KKPPPEENGDYTYVERVKIPLDHGTLLIMEGATQADW 255
[Close]
BLAST of AlkB homolog 3, alpha-ketoglutaratedependent dioxygenase vs. UniProt/SwissProt
Match: sp|Q9SIE0|ALKB2_ARATH (DNA oxidative demethylase ALKBH2 OS=Arabidopsis thaliana OX=3702 GN=ALKBH2 PE=2 SV=2)

HSP 1 Score: 90.1225 bits (222), Expect = 1.534e-18
Identity = 66/200 (33.00%), Postives = 90/200 (45.00%), Query Frame = 3 
Query:  360 TIELEKG--------FLSSDEASNLCQYFLHHLPWEQRENATPNGEKFLEPRKTCWY---GLPYSYSKIKMPPNDKWD--PKLRQLLNQVQKYNNGKPFNSVMCNLYFDNKNSIDWHADDEKSLGENPTIASISLGCSRAFEISYRKDLYVDY-KNKSKNPYRSRVKDDPPFDGCSILYRVFLGEGSLLIMSGSLQNHWL 917
            TI+L  G        FL   ++     Y   H+PW  R      G   L+PR TC+    GL         P +  WD  P L+++L+ + K   G  FNS++ N Y    + + WHADDEK  G  P IAS+S GC R F +  +KD      K     P + R+K     D  S+     L  GSLL+M G  Q  W+
Sbjct:   98 TIDLGHGSDLIYIQRFLPFQQSWTFFDYLDKHIPW-TRPTIRVFGRSCLQPRDTCYVASSGLTALVYSGYRPTSYSWDDFPPLKEILDAIYKVLPGSRFNSLLLNRYKGASDYVAWHADDEKIYGPTPEIASVSFGCERDFVLKKKKDEESSQGKTGDSGPAKKRLKRSSREDQQSLT----LKHGSLLVMRGYTQRDWI 292
[Close]
BLAST of AlkB homolog 3, alpha-ketoglutaratedependent dioxygenase vs. TrEMBL
Match: A0A3Q3EEA6 (AlkB homolog 3, alpha-ketoglutarate-dependent dioxygenase OS=Labrus bergylta OX=56723 PE=4 SV=1)

HSP 1 Score: 147.132 bits (370), Expect = 1.702e-36
Identity = 97/285 (34.04%), Postives = 142/285 (49.82%), Query Frame = 3 
Query:   75 VKDKRKANRIQGGWNKNSDSKATCKIVFEPELKSNSIDIIPTFDDRVIGEDKEKFNIIEVSENISIDINKDSLKYFTNNFPLKHYQILVDETIPATIELEKGFLSSDEASNLCQYFLHHLPWEQRENATPNGEKFLEPRKTCWYG-LPYSYSKIKMPPNDKWDPKLRQLLNQVQKYNNGKPFNSVMCNLYFDNKNSIDWHADDEKSLGENPTIASISLGCSRAFEISYRKDLYVDYKNKSKNPYRSRVKDDPPFDGCSILY----RVFLGEGSLLIMSGSLQNHW 914
            + DKR+  R+QG W K+S  K + +   +P+    S+   PT       +  + F   + ++ +     +  ++   +      Y+I    +  + + L  GFLS DEA  +    L  LPW Q+ N    GE + EPR TCWYG LPY+Y++  M PN +W P L  L   V++  +G  FNS++CNLY D  +SI WH+DDE SLG+ PTIAS+SLG  R F +                      K  PP +     Y    RV L  G+LL+MSG+ Q+ W
Sbjct:    1 MSDKRQRARVQGSWAKHSGPKGSVQNNQQPK----SVHTAPTSWGCGPQKTPQTFEYHQPAKQLREAPPEKLIENAGD------YEISCGPSGMSRLRLLPGFLSPDEADWMFSKLLAELPWSQKTNFR-QGEAYDEPRMTCWYGELPYTYARSTMAPNSEWHPLLLTLCGVVEQ-ASGCSFNSLLCNLYRDGHDSIGWHSDDEASLGDKPTIASLSLGDIRVFSLR---------------------KQPPPEENGDYTYVERIRVPLSHGTLLLMSGATQDDW 252
[Close]
BLAST of AlkB homolog 3, alpha-ketoglutaratedependent dioxygenase vs. TrEMBL
Match: F6RR85 (AlkB homolog 3, alpha-ketoglutaratedependent dioxygenase OS=Monodelphis domestica OX=13616 GN=ALKBH3 PE=4 SV=3)

HSP 1 Score: 145.591 bits (366), Expect = 7.742e-36
Identity = 93/286 (32.52%), Postives = 146/286 (51.05%), Query Frame = 3 
Query:   75 VKDKRKANRIQGGWNKNSDSKATCKIVFEPELKSNSIDIIPTFDDRVIGEDKEKFNIIEVSENISIDINKDSLKYFTNNFPLKHYQILVDETIP-ATIELEKGFLSSDEASNLCQYFLHHLPWEQRENATPNGEKFLEPRKTCWYG-LPYSYSKIKMPPNDKWDPKLRQLLNQVQKYNNGKPFNSVMCNLYFDNKNSIDWHADDEKSLGENPTIASISLGCSRAFEISYRKDLYVDYKNKSKNPYRSRVKDDPPFDGCSILY----RVFLGEGSLLIMSGSLQNHW 914
            + DKR+  R+QGGW   + S+A  +  F  +   +   +  T+  + +   +++F   E  E +SI     S+K     F L  +  +    +P   I L   F+ S+EA  + +     +PW+QR     +   +L+PR T WYG LPY+YS+I M PN +W P L  L  ++++ N+G  FNS++CNLY + K+S+DWH+DDE +LG  P IAS+SLG +R FE+                      K  PP +     Y    ++ L  G+LL+M G+ Q  W
Sbjct:    1 MDDKRRRVRVQGGWAGPAKSQAPGQPAFRAKSHPHQ-GLGQTWMKKELPLPEKQFVYEEPKEEVSIPSGSLSIK-----FSLPGFSFICYGPLPFIRIRLFPNFIESEEADWIFEQLCQDIPWKQRTGIRED-TSYLQPRLTAWYGELPYTYSRITMEPNPQWHPVLGTLKKRIEE-NSGHTFNSLLCNLYRNEKDSVDWHSDDEPALGRCPVIASLSLGATRTFEMR---------------------KKPPPEENGDYTYMEKVKIPLDHGTLLMMEGATQADW 257
[Close]
BLAST of AlkB homolog 3, alpha-ketoglutaratedependent dioxygenase vs. TrEMBL
Match: A0A5F8GHJ2 (AlkB homolog 3, alpha-ketoglutaratedependent dioxygenase OS=Monodelphis domestica OX=13616 GN=ALKBH3 PE=4 SV=1)

HSP 1 Score: 145.206 bits (365), Expect = 8.720e-36
Identity = 93/286 (32.52%), Postives = 146/286 (51.05%), Query Frame = 3 
Query:   75 VKDKRKANRIQGGWNKNSDSKATCKIVFEPELKSNSIDIIPTFDDRVIGEDKEKFNIIEVSENISIDINKDSLKYFTNNFPLKHYQILVDETIP-ATIELEKGFLSSDEASNLCQYFLHHLPWEQRENATPNGEKFLEPRKTCWYG-LPYSYSKIKMPPNDKWDPKLRQLLNQVQKYNNGKPFNSVMCNLYFDNKNSIDWHADDEKSLGENPTIASISLGCSRAFEISYRKDLYVDYKNKSKNPYRSRVKDDPPFDGCSILY----RVFLGEGSLLIMSGSLQNHW 914
            + DKR+  R+QGGW   + S+A  +  F  +   +   +  T+  + +   +++F   E  E +SI     S+K     F L  +  +    +P   I L   F+ S+EA  + +     +PW+QR     +   +L+PR T WYG LPY+YS+I M PN +W P L  L  ++++ N+G  FNS++CNLY + K+S+DWH+DDE +LG  P IAS+SLG +R FE+                      K  PP +     Y    ++ L  G+LL+M G+ Q  W
Sbjct:    1 MDDKRRRVRVQGGWAGPAKSQAPGQPAFRAKSHPHQ-GLGQTWMKKELPLPEKQFVYEEPKEEVSIPSGSLSIK-----FSLPGFSFICYGPLPFIRIRLFPNFIESEEADWIFEQLCQDIPWKQRTGIRED-TSYLQPRLTAWYGELPYTYSRITMEPNPQWHPVLGTLKKRIEE-NSGHTFNSLLCNLYRNEKDSVDWHSDDEPALGRCPVIASLSLGATRTFEMR---------------------KKPPPEENGDYTYMEKVKIPLDHGTLLMMEGATQADW 257
[Close]
BLAST of AlkB homolog 3, alpha-ketoglutaratedependent dioxygenase vs. TrEMBL
Match: A0A3B3D2H7 (AlkB homolog 3, alpha-ketoglutarate-dependent dioxygenase OS=Oryzias melastigma OX=30732 PE=4 SV=1)

HSP 1 Score: 144.05 bits (362), Expect = 3.377e-35
Identity = 96/288 (33.33%), Postives = 137/288 (47.57%), Query Frame = 3 
Query:   75 VKDKRKANRIQGGWNKNSDSKATCKIVFEPELKSNSIDIIPTFDDRVIGEDKEKFNIIEVSENISIDINKDSLKYFTNNFPLKH---YQILVDETIPATIELEKGFLSSDEASNLCQYFLHHLPWEQRENATPNGEKFLEPRKTCWYG-LPYSYSKIKMPPNDKWDPKLRQLLNQVQKYNNGKPFNSVMCNLYFDNKNSIDWHADDEKSLGENPTIASISLGCSRAFEISYRKDLYVDYKNKSKNPYRSRVKDDPPFDGCSILY----RVFLGEGSLLIMSGSLQNHW 914
            + DKR+  R+QG W K S  K + +    P+  +  I     F     G +K        S+          ++       ++H   Y+I       + + L+ GFLS++EA  +    L  LPW Q+ N    GE + EPR TCW+G LPY+Y++  MP N +W P L  L   V++   G  FNS++CNLY D  +SI WH+DDE SLG  PTIAS+SLG +R F +                      K  PP +     Y    RV L  G+LL+M G+ Q+ W
Sbjct:    1 MSDKRQRARVQGSWAKQS-PKPSGQTGIAPK-NNQPISANAAFGSWRFGNEK-------ASKAFEFHQPTKPVREVPPERVIEHAGEYEISYGPAGVSRLRLQPGFLSTEEADWMFSKLLAELPWSQKTNYR-QGEAYEEPRLTCWFGELPYTYARSTMPSNPQWPPVLETLRAAVEQ-TTGCSFNSLLCNLYRDGHDSIGWHSDDEASLGAKPTIASVSLGDTRVFSLR---------------------KQPPPEEDGDYTYVERIRVPLTHGTLLLMEGATQDDW 256
[Close]
BLAST of AlkB homolog 3, alpha-ketoglutaratedependent dioxygenase vs. TrEMBL
Match: K9IIS8 (Putative alpha-ketoglutarate-dependent dioxygenase alkb log 3 OS=Desmodus rotundus OX=9430 PE=2 SV=1)

HSP 1 Score: 143.665 bits (361), Expect = 3.413e-35
Identity = 95/295 (32.20%), Postives = 144/295 (48.81%), Query Frame = 3 
Query:   75 VKDKRKANRIQGGWNKNSDSKATCKIVFEPELKSNS----------IDIIPTFDDRVIGEDKEKFNIIEVSENISIDINKDSLKYFTNNFPLKHYQILVDETIPATIELEKGFLSSDEASNLCQYFLHHLPWEQRENATPNGEKFLEPRKTCWYG-LPYSYSKIKMPPNDKWDPKLRQLLNQVQKYNNGKPFNSVMCNLYFDNKNSIDWHADDEKSLGENPTIASISLGCSRAFEISYRKDLYVDYKNKSKNPYRSRVKDDPPFDGCSILY----RVFLGEGSLLIMSGSLQNHW 914
            ++DKR+  R+QG W  ++ S+A    V +P   S S          I+      DR     KE   ++  +    + INK+ +           Y+I +  T  + + L  GF+   EA  + +     +PW+QR     +   + +PR T WYG LPY+YS++ M PN  W P LR L +Q+++ N G  FNS++CNLY + K+S+DWH+DDE SLG  P IAS+S G +R FE+                      K  PP +     Y    ++ LG G+LL+M G+ Q  W
Sbjct:    1 MEDKRRRARVQGAWAGSAKSQA----VAQPATSSESHLHQRPGQTWINKEHHLSDRQF-VFKEPQQVVRSAPEPRV-INKEGV-----------YEISLSPTGVSRVRLYPGFVDLKEADWVLEQLCQDVPWKQRTGIRED-VTYQQPRLTAWYGELPYTYSRVTMEPNPHWHPVLRTLKSQIEE-NTGHTFNSLLCNLYRNEKDSVDWHSDDEPSLGRCPVIASLSFGATRTFEMR---------------------KKPPPEENGDYTYVERVKIPLGHGTLLVMEGATQADW 255
[Close]
BLAST of AlkB homolog 3, alpha-ketoglutaratedependent dioxygenase vs. Ensembl Cavefish
Match: alkbh3 (alkB homolog 3, alpha-ketoglutaratedependent dioxygenase [Source:NCBI gene;Acc:103026192])

HSP 1 Score: 134.42 bits (337), Expect = 1.919e-35
Identity = 92/285 (32.28%), Postives = 131/285 (45.96%), Query Frame = 3 
Query:   75 VKDKRKANRIQGGWNKNSDSKATCKIVFEPELKSNSIDIIPTFDDRVIGEDKEKFNIIEVSENISIDINKDSLKYFTNNFPLKHYQILVDETIPATIELEKGFLSSDEASNLCQYFLHHLPWEQRENATPNGEKFLEPRKTCWYG-LPYSYSKIKMPPNDKWDPKLRQLLNQVQKYNNGKPFNSVMCNLYFDNKNSIDWHADDEKSLGENPTIASISLGCSRAFEISYRKDLYVDYKNKSKNPYRSRVKDDPPFDGCSILY----RVFLGEGSLLIMSGSLQNHW 914
            + DKR+  R+QG W K +  K +  +    +    S +          G     +   + ++ + +   +  L+   +      Y+I    T  + + L  GFL  +EA  +    +  LPW Q+ N    GE + EPR TCWYG LPY+YS   M  N +W P L  L   +++      FNS++CNLY D K+SI WH+DDE SLG  PTIAS+SLG +RAF +                      K  PP +     Y    RV L  G+LLIM G  Q  W
Sbjct:    1 MGDKRQRARVQGSWAKPA-GKPSHSVAGRQQPVPGSWNT---------GNVPRTYEFHQPADPVRVIPVEKVLEEAGD------YEISQGPTGVSRLRLLPGFLPVEEADWMFARLMAELPWSQKTNYRMMGEAYEEPRLTCWYGELPYTYSHSTMEANPQWHPVLTTLRQAIER-ETCHTFNSLLCNLYRDGKDSIAWHSDDEPSLGPQPTIASLSLGDTRAFSLR---------------------KKPPPEEKGDYTYTEKLRVPLSHGTLLIMEGCTQEDW 247
[Close]
BLAST of AlkB homolog 3, alpha-ketoglutaratedependent dioxygenase vs. Ensembl Cavefish
Match: alkbh2 (alkB homolog 2, alpha-ketoglutarate dependent dioxygenase [Source:NCBI gene;Acc:103024811])

HSP 1 Score: 65.4698 bits (158), Expect = 1.656e-11
Identity = 58/203 (28.57%), Postives = 89/203 (43.84%), Query Frame = 3 
Query:  363 IELEKGFLSSDEASNLCQYFLHHLPWEQRENATPNGEKFLEPRKTCWYGLPYSYSKIKMPPNDKWDPKLRQLLNQVQKYNNGKPFNSVMCNLYFDNKNSIDWHADDEKSLGENPTIASISLGCSRAFEISYRKDLYVDYKNKSKNPYRSRVKDDPPFDGCSILYRVFLGEGSLLIMSGSLQNHWLVGFISINIYSTFPYKNKT 971
            +E+E  + + DEA       L+++P   R+ AT   E           GL YS+S + +     W P L + +  V     G+ FN V+ N Y D  + I  H DDE+ L     IAS+SLG SR F   ++     D ++ S NP + ++K            ++ L  GSLL+M+     HW         Y + P + K 
Sbjct:   81 LEVEVEYFTGDEAQVQVYGKLYNVP---RQQATYGDE-----------GLMYSFSGVHLLAR-LWTPTL-EYIRDVVTNATGQTFNFVLINRYKDGNDHIGEHRDDERELDPTCPIASVSLGASRDFIFRHK-----DARSGS-NPNKRQIKP----------VKLELAHGSLLLMNAPTNRHW---------YHSLPVRKKV 242
[Close]
BLAST of AlkB homolog 3, alpha-ketoglutaratedependent dioxygenase vs. Ensembl Sea Lamprey
Match: alkbh3 (alkB homolog 3, alpha-ketoglutarate-dependent dioxygenase [Source:ZFIN;Acc:ZDB-GENE-040801-254])

HSP 1 Score: 125.946 bits (315), Expect = 2.980e-33
Identity = 92/288 (31.94%), Postives = 133/288 (46.18%), Query Frame = 3 
Query:   81 DKRKANRIQGGW-----NKNSDSKATCKIVFEPELKSNSIDIIPTFDDRVIGEDKEKFNIIEVSENISIDINKDSLKYFTNNFPLKHYQILVDETIPATIELEKGFLSSDEASNLCQYFLHHLPWEQRENATPNGEKFLEPRKTCWYG-LPYSYSKIKMPPNDKWDPKLRQLLNQVQKYNNGKPFNSVMCNLYFDNKNSIDWHADDEKSLGENPTIASISLGCSRAFEISYRKDLYVDYKNKSKNPYRSRVKDDPPFDGCSILY----RVFLGEGSLLIMSGSLQNHW 914
            DKR   R+QGGW     N  S   A  + V +  + +   +   T D + +  D EK   +  + ++ + IN               Y+I    T  +++ L  GF+   EA  + +     +PW QR   T  G   LEPR TCWYG  PY+YS++ M  N  W P +  L +++ +   G   NSV+ N Y +  +S+DWH DDE  LG  P+IAS+SLG  R+FE+                      K  PP D     Y    RV LG G L++M G+LQ+ W
Sbjct:    2 DKRSRPRVQGGWATPAANNKSRPPAASQQVTKTPISAPKQNNAETQDGKFVYVDPEK--PLRRAPDVKV-INAAG-----------DYEISTGPTGVSSLRLFPGFVERAEADWMFEQLAAEIPWGQR-TYTKAGVTSLEPRLTCWYGDHPYTYSRLTMEANPHWHPLVTMLRDRIAEVT-GVQCNSVLANWYRNEHDSVDWHCDDEPDLGPQPSIASLSLGEMRSFELR---------------------KKPPPEDEGDFTYVERLRVPLGHGDLILMFGALQDDW 252
[Close]
BLAST of AlkB homolog 3, alpha-ketoglutaratedependent dioxygenase vs. Ensembl Nematostella
Match: EDO39194 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SAR6])

HSP 1 Score: 120.939 bits (302), Expect = 5.760e-31
Identity = 92/284 (32.39%), Postives = 131/284 (46.13%), Query Frame = 3 
Query:   81 DKRKANRIQGGWNKNSDSKATCKIVFEPELKSNSIDIIP---TFDDRVIGEDKEKFNIIEVSENISIDINKDSLKYFTNNFPLKHYQILVDETIPATIELEKGFLSSDEASNLCQYFLHHLPWEQRENATPNGEKFLEPRKTCWYG-LPYSYSKIKMPPNDKWDPKLRQLLNQVQKYNNGKPFNSVMCNLYFDNKNSIDWHADDEKSLGENPTIASISLGCSRAFEISYRKDLYVDYKNKSKNPYRSRVKDDPPFDGCSILY--RVFLGEGSLLIMSGSLQNHW 914
            DKR+  RIQG W       +      + E  S S D       F   V+   ++   +  ++E  +                   Y+I    +  A I+L   FL  DE   + +     +PWE+++     GE   +PR T W+G  PY+YS + + P  +W P L  L  ++ K   G+ FNS++ NLY  NK+S+DWHADDE SLG NPTIAS+S G SR FE+              KNP           D  S++   +V L  GSLL+M GS+Q  W
Sbjct:    2 DKRRKKRIQGSWAGKMAKPSNKTATSQEEQASGSNDPSEKKFVFQGNVVNTIRQAPPVKTLTERGT-------------------YEISTGPSGLAIIDLYPSFLDGDETEWMFEQLQAEIPWEEKDIKI-KGEFHKQPRLTAWFGEFPYTYSGLTLRPF-QWSPILNILREKIAKAT-GETFNSMLANLYRHNKDSVDWHADDEPSLGVNPTIASLSFGDSRVFEL-------------RKNPLNE-------GDDYSLMQHIKVPLNCGSLLVMRGSVQEDW 243
[Close]
BLAST of AlkB homolog 3, alpha-ketoglutaratedependent dioxygenase vs. Ensembl Nematostella
Match: EDO42093 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S2D3])

HSP 1 Score: 48.521 bits (114), Expect = 1.673e-6
Identity = 40/146 (27.40%), Postives = 63/146 (43.15%), Query Frame = 3 
Query:  486 PRKTCWYG---LPYSYSKIKMPPNDKWDPKLRQLLNQVQKYNNGKPFNSVMCNLYFDNKNSIDWHADDEKSLGENPTIASISLGCSRAFEISYRKDLYVDYKNKSKNPYRSRVKDDPPFDGCSILYRVFLGEGSLLIMSGSLQNHW 914
            PR+   +G   L Y++S + + P    +    Q L        G+ FN V+ N Y D  + +  H DDEK L  +  IAS+S G +R F           +++K      S    DP         ++ L  GSLL+M+    ++W
Sbjct:   60 PRRQTSFGDEGLKYTFSGVTVTPQHWKEAPFLQDLRDYLVDITGQTFNFVLVNRYKDGNDHMGEHRDDEKELVRSSPIASLSFGQARDFV----------FRHKDCRGRTSTRNIDP--------VKLELQNGSLLLMNHPTNHYW 187
[Close]
BLAST of AlkB homolog 3, alpha-ketoglutaratedependent dioxygenase vs. Ensembl Medaka
Match: alkbh3 (alkB homolog 3, alpha-ketoglutaratedependent dioxygenase [Source:NCBI gene;Acc:101164226])

HSP 1 Score: 140.198 bits (352), Expect = 2.453e-37
Identity = 98/300 (32.67%), Postives = 134/300 (44.67%), Query Frame = 3 
Query:   75 VKDKRKANRIQGGWNKNSDSKATCKIVFEPELKSNSIDIIPTFDDRVIGEDKEKFNIIEVSENISIDINKDSLKYFTNNFPLK---------------HYQILVDETIPATIELEKGFLSSDEASNLCQYFLHHLPWEQRENATPNGEKFLEPRKTCWYG-LPYSYSKIKMPPNDKWDPKLRQLLNQVQKYNNGKPFNSVMCNLYFDNKNSIDWHADDEKSLGENPTIASISLGCSRAFEISYRKDLYVDYKNKSKNPYRSRVKDDPPFDGCSILY----RVFLGEGSLLIMSGSLQNHW 914
            + DKR+  R+QG W K S                      P+    +  ++ +  N      +   D N+ + K F  + P K                Y I    +  + + L  GFLS++EA  +    L  LPW Q+ N    GE + EPR TCWYG LPY+Y +  MP N +W P L  L   V++   G  FNS++CNLY D  +SI WH+DDE SLG  PTIAS+SLG SR F +                      K  PP +     Y    RV L  G+LL+M G+ Q+ W
Sbjct:    1 MSDKRQRARVQGSWAKQSPK--------------------PSGQTGIALKNNQSVNAAATVGSWGFD-NEKTPKAFEFHQPTKPVREVPPERVIEQAGEYDISYGPSGVSRLRLLPGFLSTEEADWMFSKLLAELPWSQKTNYR-EGEAYEEPRLTCWYGELPYTYGRSTMPSNSQWHPVLETLRAAVEQ-ATGCSFNSLLCNLYRDGHDSIGWHSDDEASLGAKPTIASLSLGDSRVFSLR---------------------KQPPPEENGDYTYVERIRVPLTHGALLLMEGATQDDW 256
[Close]
BLAST of AlkB homolog 3, alpha-ketoglutaratedependent dioxygenase vs. Ensembl Medaka
Match: alkbh2 (alkB homolog 2, alpha-ketoglutarate dependent dioxygenase [Source:NCBI gene;Acc:110015618])

HSP 1 Score: 60.077 bits (144), Expect = 9.013e-10
Identity = 58/205 (28.29%), Postives = 88/205 (42.93%), Query Frame = 3 
Query:  381 FLSSDEASNLCQYFLHHLPWEQRENATPN--GEKFLEPRKTCWYG---LPYSYS---KIKMPPNDKWDPKLRQLLNQVQKYNNGKPFNSVMCNLYFDNKNSIDWHADDEKSLGENPTIASISLGCSRAFEISYRKDLYVDYKNKSKNPYRSRVKDDPPFDGCSILYRVFLGEGSLLIMSGSLQNHWLVGFISINIYSTFPYKNKT 971
              S  EA  L ++    + +   E A     G+ +  PRK   YG   L Y+YS   ++  P    W P L  + + V K   G+ FN V+ N Y D  + +  H DDEK L  +  IAS+SLG +R F   +R         + +N  R   + +P         ++ L  GSLL+M+    + W         Y + P + KT
Sbjct:   46 IFSKKEADELFKHLEEEVVYSTGEEAKVQVYGKVYNIPRKQATYGDTGLMYTYSGVTRLACP----WTPTLENIRDAVTK-ATGQTFNFVLINRYKDGLDHMGEHRDDEKELDPSCPIASVSLGAARDFVFRHR-------DTRGRNSSR---QIEP--------VKLELSHGSLLLMNPPTNHFW---------YHSLPVRRKT 218
[Close]
BLAST of AlkB homolog 3, alpha-ketoglutaratedependent dioxygenase vs. Ensembl Medaka
Match: alkbh2 (alkB homolog 2, alpha-ketoglutarate dependent dioxygenase [Source:NCBI gene;Acc:110015618])

HSP 1 Score: 60.077 bits (144), Expect = 1.625e-9
Identity = 58/205 (28.29%), Postives = 88/205 (42.93%), Query Frame = 3 
Query:  381 FLSSDEASNLCQYFLHHLPWEQRENATPN--GEKFLEPRKTCWYG---LPYSYS---KIKMPPNDKWDPKLRQLLNQVQKYNNGKPFNSVMCNLYFDNKNSIDWHADDEKSLGENPTIASISLGCSRAFEISYRKDLYVDYKNKSKNPYRSRVKDDPPFDGCSILYRVFLGEGSLLIMSGSLQNHWLVGFISINIYSTFPYKNKT 971
              S  EA  L ++    + +   E A     G+ +  PRK   YG   L Y+YS   ++  P    W P L  + + V K   G+ FN V+ N Y D  + +  H DDEK L  +  IAS+SLG +R F   +R         + +N  R   + +P         ++ L  GSLL+M+    + W         Y + P + KT
Sbjct:  110 IFSKKEADELFKHLEEEVVYSTGEEAKVQVYGKVYNIPRKQATYGDTGLMYTYSGVTRLACP----WTPTLENIRDAVTK-ATGQTFNFVLINRYKDGLDHMGEHRDDEKELDPSCPIASVSLGAARDFVFRHR-------DTRGRNSSR---QIEP--------VKLELSHGSLLLMNPPTNHFW---------YHSLPVRRKT 282
[Close]
BLAST of AlkB homolog 3, alpha-ketoglutaratedependent dioxygenase vs. Planmine SMEST
Match: SMESG000044993.1 (SMESG000044993.1)

HSP 1 Score: 581.252 bits (1497), Expect = 0.000e+0
Identity = 281/284 (98.94%), Postives = 283/284 (99.65%), Query Frame = 3 
Query:   66 MSAVKDKRKANRIQGGWNKNSDSKATCKIVFEPELKSNSIDIIPTFDDRVIGEDKEKFNIIEVSENISIDINKDSLKYFTNNFPLKHYQILVDETIPATIELEKGFLSSDEASNLCQYFLHHLPWEQRENATPNGEKFLEPRKTCWYGLPYSYSKIKMPPNDKWDPKLRQLLNQVQKYNNGKPFNSVMCNLYFDNKNSIDWHADDEKSLGENPTIASISLGCSRAFEISYRKDLYVDYKNKSKNPYRSRVKDDPPFDGCSILYRVFLGEGSLLIMSGSLQNHWL 917
            MSAVKDKRKANRIQGGWNKNSDSKATCKIVFEPELKSNSIDIIPTFDDRVIGEDKEKFNIIEVSENIS+DINKDSLKYFTNNFPLKHYQILVDETIPATIELEKGFLSSDEASNL QYFLHHLPWEQRENATPNGEKFLEPRKTCWYGLPYSYSKIKMPPNDKWDPKLRQLL+QVQKYNNGKPFNSVMCNLYFDNKNSIDWHADDEKSLGENPTIASISLGCSRAFEISYRKDLYVDYKNKSKNPYRSRVKDDPPFDGCSILYRVFLGEGSLLIMSGSLQNHWL
Sbjct:    1 MSAVKDKRKANRIQGGWNKNSDSKATCKIVFEPELKSNSIDIIPTFDDRVIGEDKEKFNIIEVSENISLDINKDSLKYFTNNFPLKHYQILVDETIPATIELEKGFLSSDEASNLYQYFLHHLPWEQRENATPNGEKFLEPRKTCWYGLPYSYSKIKMPPNDKWDPKLRQLLDQVQKYNNGKPFNSVMCNLYFDNKNSIDWHADDEKSLGENPTIASISLGCSRAFEISYRKDLYVDYKNKSKNPYRSRVKDDPPFDGCSILYRVFLGEGSLLIMSGSLQNHWL 284
[Close]
BLAST of AlkB homolog 3, alpha-ketoglutaratedependent dioxygenase vs. Planmine SMEST
Match: SMESG000025916.1 (SMESG000025916.1)

HSP 1 Score: 167.548 bits (423), Expect = 3.708e-45
Identity = 90/202 (44.55%), Postives = 119/202 (58.91%), Query Frame = 3 
Query:  333 ILVDETIPATIELEKGFLSSDEASNLCQYFLHHLPWEQRENATPNGEKFLEPRKTCWYGLPYSYSKIKMPPNDKWDP---KLRQLLNQVQKYNNGKPFNSVMCNLYFDNKNSIDWHADDEKSLGENPTIASISLGCSRAFEISYRKDLYVDYKNKSKNPYRSRVKDDPP----FDGCSILYRVFLGEGSLLIMSGSLQNHWL 917
            ++  E +P  I+    ++SS+EA +L +Y   H+ W+QRE    NG+ + EPR T W+G PY+YSK+K   N  WD    KLR  L +    ++ K +NSVMCN Y +N +SI WH+DDE SLG  P IASISLGC R FE+  R++ Y            SR   D P    F GC+ILYR  L  GSLLIM GS+Q  +L
Sbjct:   60 LITIEGVPGRIQYFPNYISSEEADSLYKYLTEHINWKQREGTNQNGDTYYEPRLTYWFGKPYTYSKVKWESNQNWDKEIDKLRVRLTKEHPIDDQKEYNSVMCNYYRNNIDSIGWHSDDEMSLGRFPKIASISLGCQRVFEVCRREEYY------------SRGFYDGPRNSVFSGCTILYRFHLKPGSLLIMEGSIQEEFL 249
The following BLAST results are available for this feature:
BLAST of AlkB homolog 3, alpha-ketoglutaratedependent dioxygenase vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ALKBH38.064e-3430.00alkB homolog 3, alpha-ketoglutaratedependent dioxy... [more]
ALKBH33.003e-3133.19alkB homolog 3, alpha-ketoglutaratedependent dioxy... [more]
ALKBH36.617e-2037.06alkB homolog 3, alpha-ketoglutaratedependent dioxy... [more]
ALKBH23.840e-1130.30alkB homolog 2, alpha-ketoglutarate dependent diox... [more]
ALKBH23.840e-1130.30alkB homolog 2, alpha-ketoglutarate dependent diox... [more]
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BLAST of AlkB homolog 3, alpha-ketoglutaratedependent dioxygenase vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of AlkB homolog 3, alpha-ketoglutaratedependent dioxygenase vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of AlkB homolog 3, alpha-ketoglutaratedependent dioxygenase vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
alkbh38.780e-3332.86alkB homolog 3, alpha-ketoglutarate-dependent diox... [more]
alkbh38.780e-3332.86alkB homolog 3, alpha-ketoglutarate-dependent diox... [more]
alkbh38.780e-3332.86alkB homolog 3, alpha-ketoglutarate-dependent diox... [more]
alkbh39.307e-1931.47alkB homolog 3, alpha-ketoglutarate-dependent diox... [more]
alkbh21.201e-728.29alkB homolog 2, alpha-ketoglutarate-dependent diox... [more]
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BLAST of AlkB homolog 3, alpha-ketoglutaratedependent dioxygenase vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 4
Match NameE-valueIdentityDescription
ALKBH31.212e-3028.77alkB homolog 3, alpha-ketoglutarate-dependent diox... [more]
ALKBH34.574e-2830.42alkB homolog 3, alpha-ketoglutarate-dependent diox... [more]
g6pc.14.791e-932.53glucose-6-phosphatase, catalytic subunit, gene 1 [... [more]
g6pc.11.698e-832.53glucose-6-phosphatase, catalytic subunit, gene 1 [... [more]
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BLAST of AlkB homolog 3, alpha-ketoglutaratedependent dioxygenase vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Alkbh31.548e-3229.83alkB homolog 3, alpha-ketoglutarate-dependent diox... [more]
Alkbh37.956e-3229.76alkB homolog 3, alpha-ketoglutarate-dependent diox... [more]
Alkbh36.738e-2330.19alkB homolog 3, alpha-ketoglutarate-dependent diox... [more]
Gm270277.968e-1444.74predicted gene, 27027 [Source:MGI Symbol;Acc:MGI:5... [more]
Alkbh24.383e-1132.88alkB homolog 2, alpha-ketoglutarate-dependent diox... [more]
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BLAST of AlkB homolog 3, alpha-ketoglutaratedependent dioxygenase vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q32L00|ALKB3_BOVIN4.678e-3532.19Alpha-ketoglutarate-dependent dioxygenase alkB hom... [more]
sp|Q96Q83|ALKB3_HUMAN3.873e-3330.00Alpha-ketoglutarate-dependent dioxygenase alkB hom... [more]
sp|Q5XIC8|ALKB3_RAT6.094e-3229.69Alpha-ketoglutarate-dependent dioxygenase alkB hom... [more]
sp|Q8K1E6|ALKB3_MOUSE1.083e-3129.83Alpha-ketoglutarate-dependent dioxygenase alkB hom... [more]
sp|Q9SIE0|ALKB2_ARATH1.534e-1833.00DNA oxidative demethylase ALKBH2 OS=Arabidopsis th... [more]
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BLAST of AlkB homolog 3, alpha-ketoglutaratedependent dioxygenase vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A3Q3EEA61.702e-3634.04AlkB homolog 3, alpha-ketoglutarate-dependent diox... [more]
F6RR857.742e-3632.52AlkB homolog 3, alpha-ketoglutaratedependent dioxy... [more]
A0A5F8GHJ28.720e-3632.52AlkB homolog 3, alpha-ketoglutaratedependent dioxy... [more]
A0A3B3D2H73.377e-3533.33AlkB homolog 3, alpha-ketoglutarate-dependent diox... [more]
K9IIS83.413e-3532.20Putative alpha-ketoglutarate-dependent dioxygenase... [more]
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BLAST of AlkB homolog 3, alpha-ketoglutaratedependent dioxygenase vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
alkbh31.919e-3532.28alkB homolog 3, alpha-ketoglutaratedependent dioxy... [more]
alkbh21.656e-1128.57alkB homolog 2, alpha-ketoglutarate dependent diox... [more]
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BLAST of AlkB homolog 3, alpha-ketoglutaratedependent dioxygenase vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
alkbh32.980e-3331.94alkB homolog 3, alpha-ketoglutarate-dependent diox... [more]
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BLAST of AlkB homolog 3, alpha-ketoglutaratedependent dioxygenase vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of AlkB homolog 3, alpha-ketoglutaratedependent dioxygenase vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 2
Match NameE-valueIdentityDescription
EDO391945.760e-3132.39Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO420931.673e-627.40Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of AlkB homolog 3, alpha-ketoglutaratedependent dioxygenase vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 3
Match NameE-valueIdentityDescription
alkbh32.453e-3732.67alkB homolog 3, alpha-ketoglutaratedependent dioxy... [more]
alkbh29.013e-1028.29alkB homolog 2, alpha-ketoglutarate dependent diox... [more]
alkbh21.625e-928.29alkB homolog 2, alpha-ketoglutarate dependent diox... [more]
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BLAST of AlkB homolog 3, alpha-ketoglutaratedependent dioxygenase vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000044993.10.000e+098.94SMESG000044993.1[more]
SMESG000025916.13.708e-4544.55SMESG000025916.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30028242 ID=SMED30028242|Name=AlkB homolog 3, alpha-ketoglutaratedependent dioxygenase|organism=Schmidtea mediterranea sexual|type=transcript|length=1596bp
TTAAATCTAAAATTTTTTGAATAATATTTAAACTTCTTAATTCTTAGAAT
TATCAAAATTTAAAAATGAGTGCAGTGAAAGACAAACGTAAAGCTAATCG
AATTCAAGGTGGTTGGAATAAAAATTCTGATTCTAAAGCAACATGCAAAA
TAGTATTTGAACCAGAACTTAAATCAAACAGTATAGATATTATTCCTACA
TTTGATGATAGAGTTATCGGAGAAGATAAAGAAAAATTTAATATAATTGA
AGTTTCTGAAAATATATCAATAGACATAAATAAAGATTCTCTAAAGTATT
TCACTAATAATTTTCCCTTGAAGCACTACCAAATACTAGTTGATGAAACA
ATTCCTGCAACGATTGAATTAGAGAAAGGTTTTCTATCTTCTGATGAAGC
TTCAAATTTGTGCCAATATTTTTTACATCATTTGCCATGGGAGCAACGTG
AAAATGCTACACCTAATGGAGAAAAGTTTCTTGAACCACGAAAAACATGC
TGGTATGGATTGCCTTATTCTTATTCAAAAATTAAAATGCCTCCAAATGA
TAAATGGGATCCAAAATTGAGACAATTATTGAACCAAGTTCAAAAATATA
ATAATGGAAAACCGTTCAATAGTGTCATGTGCAATCTATATTTTGACAAT
AAGAATTCCATAGATTGGCATGCAGACGATGAGAAAAGTTTAGGCGAAAA
TCCAACAATCGCAAGTATTTCACTTGGATGCTCTCGTGCTTTCGAAATTA
GTTATCGGAAGGATTTATACGTAGATTATAAAAACAAGTCAAAAAATCCA
TATCGCAGTCGAGTCAAAGACGATCCTCCGTTTGACGGTTGTTCTATTCT
CTATCGAGTTTTTCTGGGAGAAGGCAGTCTTTTGATAATGTCTGGTTCAT
TGCAAAATCATTGGCTGGTTGGTTTTATTTCTATTAATATTTATTCTACC
TTTCCATATAAAAATAAAACAACTGGTGTAACATGTATTTTCTTTCTATT
AATTGAAAGATATTTTATAAATTATAAGTTTTTTGCCTGTTTAACTCATA
GATTGGTGAGTTGTATATTTTCAACGTATATCCTACATTATGTTTCTTAA
TACCCCCAACAGTAATTATTATCGCTATAACTATCCGTATATAGTTCAAT
CCATCATATTAGTTTTTATTCCACCAATTCTCCAGCTCCTTTGCTATTAA
AAAGTTCCACCAATTAAATACCGAAAAATGGTGATTTCTTAATTGTGTTG
CCAAATTTTGCAATTATTTTCACATTTTTTCTTTTTGAAATACACAGACG
CGTATTTAATAGCATTGATTAAGATATTTAATAATAACAATTTAAATCAA
CCAAAGTAAGTTTGCTATTATAATTAACCAATCAAATCCCGATAACCAAT
TAGCAGATTTTCTAAATATGACAATGACTTACTGTTGCTTTATCATCGGT
CGAAACGATTCGTTTAATATTAAGCATATTTTTAGACATGAAAAGTAGAA
TCATACTGCAATGTAAATTAAACAGAAATATATTTCGTTTTAATCCTAAA
ATCAAGATCAGTTTTAATTCGTATACTCCATTACAAGAGTTTCCTG
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protein sequence of SMED30028242-orf-1

>SMED30028242-orf-1 ID=SMED30028242-orf-1|Name=SMED30028242-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=345bp
MSAVKDKRKANRIQGGWNKNSDSKATCKIVFEPELKSNSIDIIPTFDDRV
IGEDKEKFNIIEVSENISIDINKDSLKYFTNNFPLKHYQILVDETIPATI
ELEKGFLSSDEASNLCQYFLHHLPWEQRENATPNGEKFLEPRKTCWYGLP
YSYSKIKMPPNDKWDPKLRQLLNQVQKYNNGKPFNSVMCNLYFDNKNSID
WHADDEKSLGENPTIASISLGCSRAFEISYRKDLYVDYKNKSKNPYRSRV
KDDPPFDGCSILYRVFLGEGSLLIMSGSLQNHWLVGFISINIYSTFPYKN
KTTGVTCIFFLLIERYFINYKFFACLTHRLVSCIFSTYILHYVS*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: cellular component
TermDefinition
GO:0005654nucleoplasm
GO:0005739mitochondrion
GO:0005829cytosol
Vocabulary: molecular function
TermDefinition
GO:0008198ferrous iron binding
GO:0043734DNA-N1-methyladenine dioxygenase activity
GO:1990930RNA N1-methyladenosine dioxygenase activity
GO:0016491oxidoreductase activity
GO:0051213dioxygenase activity
Vocabulary: biological process
TermDefinition
GO:0008283cell proliferation
GO:0035553oxidative single-stranded RNA demethylation
GO:0055114oxidation-reduction process
GO:0006281DNA repair
GO:0006307DNA dealkylation involved in DNA repair
GO:0035552oxidative single-stranded DNA demethylation
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000070intestinal phagocyte
PLANA:0000101muscle cell
PLANA:0000142posterior region of the whole animal
Vocabulary: INTERPRO
TermDefinition
IPR037151AlkB-like_sf
IPR032854ALKBH3
IPR027450AlkB-like
IPR005123Oxoglu/Fe-dep_dioxygenase
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR027450Alpha-ketoglutarate-dependent dioxygenase AlkB-likePFAMPF135322OG-FeII_Oxy_2coord: 104..283
e-value: 2.2E-17
score: 63.9
IPR037151Alpha-ketoglutarate-dependent dioxygenase AlkB-like superfamilyGENE3DG3DSA:2.60.120.590coord: 85..286
e-value: 3.1E-43
score: 150.0
NoneNo IPR availablePANTHERPTHR31212:SF4ALPHA-KETOGLUTARATE-DEPENDENT DIOXYGENASE ALKB HOMOLOG 3coord: 92..283
NoneNo IPR availableSUPERFAMILYSSF51197Clavaminate synthase-likecoord: 86..283
IPR032854Alkylated DNA repair protein alkB homologue 3PANTHERPTHR31212FAMILY NOT NAMEDcoord: 92..283
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 183..344
score: 8.683