SMED30028234

Overview


Name	SMED30028234
Smed ID	SMED30028234
Length (bp)	1963

Neoblast Clusters

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of SMED30028234 (SMED30028234) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30028234 (SMED30028234) in cells (dots) of each of the 12 neoblast clusters.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30028234

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 4

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
reproductive organ	SMED30028234	h1SMcG0000645	Contig11314	newmark_ests	PMID:28434803 Rouhana et al., 2017
reproductive organ	SMED30028234	h1SMcG0000645	Contig11314	uc_Smed_v2	PMID:28434803 Rouhana et al., 2017
reproductive organ	SMED30028234	h1SMcG0000645	Contig30650	newmark_ests	PMID:28434803 Rouhana et al., 2017
reproductive organ	SMED30028234	h1SMcG0000645	Contig30650	uc_Smed_v2	PMID:28434803 Rouhana et al., 2017

Note: Hover over icons to view figure legend

Homology

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BLAST of SMED30028234 vs. Ensembl Human
Match: LCA5L (lebercilin LCA5 like [Source:HGNC Symbol;Acc:HGNC:1255])

HSP 1 Score: 77.411 bits (189), Expect = 3.813e-14
Identity = 91/313 (29.07%), Postives = 171/313 (54.63%), Query Frame = 3

Query:  357 TQKLLEASQLEINELISENADLRRQLLESSNELKTVQNQ--NKIQERYLNKVNSRDS---DVPTIISRYNSELHSLQEQLRRKKEKLDTVERQKTDLDFXXXXXXXXXXXXXXXXXDRQLQEREDLSKKIANLNESLEAEIKKNSDLERSMELLTKKYSYQMALETNKFKELKQSYTETL-MELKNIRSSLKEKNKLLEMQNIYSKRVVRAPH------RLSGKKGKNVTLEDSNFPVSKI-------STVPPLKSSLRKEERHNSEHEEASQIK------INLKKKYQVSVKKIENLS----HTAIQHELEN 1208
              ++L A   +I  L +E AD+  +L     E    +NQ   ++Q R+L  +   ++   ++P I++++ +E+ +L++ LR+ +EK  T+ R+  + D +L+K K  ++ L+ L  D+ L ERE+L+ K++ +   ++A  KK   LE+ + L  + +S Q+A+ET K     Q+ T+TL +E+K+++  LKEK++ LE++NIYS R+++  H      ++S    K+V  +    P + +       S VPPL +  +K   +    E++++I       +N   K + S +K E+LS    H  +Q  LEN
Sbjct:  140 AHRILSARLHKIKGLKNELADMHHKL-----EAILTENQFLKQLQLRHLKAIGKYENSQNNLPQIMAKHQNEVKNLRQLLRKSQEKERTLSRKLRETDSQLLKTKDILQALQKLSEDKNLAEREELTHKLSIITTKMDANDKKIQSLEKQLRLNCRAFSRQLAIETRK-TLAAQTATKTLQVEVKHLQQKLKEKDRELEIKNIYSHRILKNLHDTEDYPKVSST--KSVQADRKILPFTSMRHQGTQKSDVPPLTTKGKKATGNIDHKEKSTEINHEIPHCVNKLPKQEDSKRKYEDLSGEEKHLEVQILLEN 444

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Query:  357 TQKLLEASQLEINELISENADLRRQLLESSNELKTVQNQ--NKIQERYLNKVNSRDS---DVPTIISRYNSELHSLQEQLRRKKEKLDTVERQKTDLDFXXXXXXXXXXXXXXXXXDRQLQEREDLSKKIANLNESLEAEIKKNSDLERSMELLTKKYSYQMALETNKFKELKQSYTETL-MELKNIRSSLKEKNKLLEMQNIYSKRVVRAPH------RLSGKKGKNVTLEDSNFPVSKI-------STVPPLKSSLRKEERHNSEHEEASQIK------INLKKKYQVSVKKIENLS----HTAIQHELEN 1208
              ++L A   +I  L +E AD+  +L     E    +NQ   ++Q R+L  +   ++   ++P I++++ +E+ +L++ LR+ +EK  T+ R+  + D +L+K K  ++ L+ L  D+ L ERE+L+ K++ +   ++A  KK   LE+ + L  + +S Q+A+ET K     Q+ T+TL +E+K+++  LKEK++ LE++NIYS R+++  H      ++S    K+V  +    P + +       S VPPL +  +K   +    E++++I       +N   K + S +K E+LS    H  +Q  LEN
Sbjct:  140 AHRILSARLHKIKGLKNELADMHHKL-----EAILTENQFLKQLQLRHLKAIGKYENSQNNLPQIMAKHQNEVKNLRQLLRKSQEKERTLSRKLRETDSQLLKTKDILQALQKLSEDKNLAEREELTHKLSIITTKMDANDKKIQSLEKQLRLNCRAFSRQLAIETRK-TLAAQTATKTLQVEVKHLQQKLKEKDRELEIKNIYSHRILKNLHDTEDYPKVSST--KSVQADRKILPFTSMRHQGTQKSDVPPLTTKGKKATGNIDHKEKSTEINHEIPHCVNKLPKQEDSKRKYEDLSGEEKHLEVQILLEN 444

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Query:  357 TQKLLEASQLEINELISENADLRRQLLESSNELKTVQNQ--NKIQERYLNKVNSRDS---DVPTIISRYNSELHSLQEQLRRKKEKLDTVERQKTDLDFXXXXXXXXXXXXXXXXXDRQLQEREDLSKKIANLNESLEAEIKKNSDLERSMELLTKKYSYQMALETNKFKELKQSYTETL-MELKNIRSSLKEKNKLLEMQNIYSKRVVRAPH------RLSGKKGKNVTLEDSNFPVSKI-------STVPPLKSSLRKEERHNSEHEEASQIK------INLKKKYQVSVKKIENLS----HTAIQHELEN 1208
              ++L A   +I  L +E AD+  +L     E    +NQ   ++Q R+L  +   ++   ++P I++++ +E+ +L++ LR+ +EK  T+ R+  + D +L+K K  ++ L+ L  D+ L ERE+L+ K++ +   ++A  KK   LE+ + L  + +S Q+A+ET K     Q+ T+TL +E+K+++  LKEK++ LE++NIYS R+++  H      ++S    K+V  +    P + +       S VPPL +  +K   +    E++++I       +N   K + S +K E+LS    H  +Q  LEN
Sbjct:  140 AHRILSARLHKIKGLKNELADMHHKL-----EAILTENQFLKQLQLRHLKAIGKYENSQNNLPQIMAKHQNEVKNLRQLLRKSQEKERTLSRKLRETDSQLLKTKDILQALQKLSEDKNLAEREELTHKLSIITTKMDANDKKIQSLEKQLRLNCRAFSRQLAIETRK-TLAAQTATKTLQVEVKHLQQKLKEKDRELEIKNIYSHRILKNLHDTEDYPKVSST--KSVQADRKILPFTSMRHQGTQKSDVPPLTTKGKKATGNIDHKEKSTEINHEIPHCVNKLPKQEDSKRKYEDLSGEEKHLEVQILLEN 444

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BLAST of SMED30028234 vs. Ensembl Human
Match: LCA5 (lebercilin LCA5 [Source:HGNC Symbol;Acc:HGNC:31923])

HSP 1 Score: 70.8626 bits (172), Expect = 2.816e-12
Identity = 64/205 (31.22%), Postives = 121/205 (59.02%), Query Frame = 3

Query:  357 TQKLLEASQLEINELISENADLRRQLLESSNELKTVQNQNKIQERYLNKVNSRDSDVPTIISRYNSELHSLQEQLRRKKEKLDTVERQKTDLDFXXXXXXXXXXXXXXXXXDRQLQEREDLSKKIANLNESLEAEIKKNSDLERSMELLTKKYSYQMALETNKFKELKQSYTETLMELKNIRSSLKEKNKLLEMQNIYSKRVVRA 971
            T+++L A  L+INEL +E ++L+ +L E   E K+++     QE+ LNK    ++++  +I R+N+E+ +L+E+LR+ +EK    E++  D + EL + K  ++KLK +   R L ER+DL+KK+ +    L+   ++  +L +++EL T  +  Q+  E  +  E          E++ +   LKEK + L+++NIYS R+ ++
Sbjct:  101 TKRILSARLLKINELQNEVSELQVKLAELLKENKSLKRLQYRQEKALNKFEDAENEISQLIFRHNNEITALKERLRKSQEKERATEKRVKDTESELFRTKFSLQKLKEISEARHLPERDDLAKKLVSAELKLDDTERRIKELSKNLELSTNSFQRQLLAERKRAYEAHDENKVLQKEVQRLYHKLKEKERELDIKNIYSNRLPKS 305

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BLAST of SMED30028234 vs. Ensembl Human
Match: LCA5 (lebercilin LCA5 [Source:HGNC Symbol;Acc:HGNC:31923])

HSP 1 Score: 71.2478 bits (173), Expect = 3.632e-12
Identity = 64/205 (31.22%), Postives = 121/205 (59.02%), Query Frame = 3

Query:  357 TQKLLEASQLEINELISENADLRRQLLESSNELKTVQNQNKIQERYLNKVNSRDSDVPTIISRYNSELHSLQEQLRRKKEKLDTVERQKTDLDFXXXXXXXXXXXXXXXXXDRQLQEREDLSKKIANLNESLEAEIKKNSDLERSMELLTKKYSYQMALETNKFKELKQSYTETLMELKNIRSSLKEKNKLLEMQNIYSKRVVRA 971
            T+++L A  L+INEL +E ++L+ +L E   E K+++     QE+ LNK    ++++  +I R+N+E+ +L+E+LR+ +EK    E++  D + EL + K  ++KLK +   R L ER+DL+KK+ +    L+   ++  +L +++EL T  +  Q+  E  +  E          E++ +   LKEK + L+++NIYS R+ ++
Sbjct:  101 TKRILSARLLKINELQNEVSELQVKLAELLKENKSLKRLQYRQEKALNKFEDAENEISQLIFRHNNEITALKERLRKSQEKERATEKRVKDTESELFRTKFSLQKLKEISEARHLPERDDLAKKLVSAELKLDDTERRIKELSKNLELSTNSFQRQLLAERKRAYEAHDENKVLQKEVQRLYHKLKEKERELDIKNIYSNRLPKS 305

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BLAST of SMED30028234 vs. Ensembl Zebrafish
Match: lca5 (lebercilin LCA5 [Source:ZFIN;Acc:ZDB-GENE-090313-268])

HSP 1 Score: 85.5001 bits (210), Expect = 7.836e-17
Identity = 72/223 (32.29%), Postives = 129/223 (57.85%), Query Frame = 3

Query:  357 TQKLLEASQLEINELISENADLRRQLLESSNELKTVQNQNKIQERYLNKVNSRDSDVPTIISRYNSELHSLQEQLRRKKEKLDTVERQKTDLDFXXXXXXXXXXXXXXXXXDRQLQEREDLSKKIANLNESLEAEIKKNSDLERSMELLTKKYSYQMALETNKFKELKQSYTETLMELKNIRSSLKEKNKLLEMQNIYSKRVVRAPHR-----LSGKKGKNVT 1010
            T+++L A  L+INEL +  ++LR    E   E + ++     QE+ L++ N  DS++  +++R+N+E H L+E+LRR +E   T ER   + D +L + + +++KL+ L  D+ L ERE+LS+K+      L+    +  +LE++MEL +  +  Q+A E  K  + +Q       E++ + + LKEK + L+ +NIY+ R+ +A  R      S +KG N+T
Sbjct:  155 TKRMLSARLLKINELKNALSELRLHADELQRENRLLRQLQLRQEKALHRYNDTDSEISQLLARHNNETHVLRERLRRSQENQRTAERSLRERDAQLQRCRSQMQKLQQLADDQNLGEREELSRKLIGTQSRLQDSEHRVKELEKNMELSSGSFQRQLANERRKTHDAQQEVKALQEEVERLCTKLKEKERELDARNIYANRMHKASSRKEAENGSKRKGFNIT 377

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BLAST of SMED30028234 vs. Ensembl Zebrafish
Match: lca5 (lebercilin LCA5 [Source:ZFIN;Acc:ZDB-GENE-090313-268])

HSP 1 Score: 85.5001 bits (210), Expect = 8.400e-17
Identity = 72/223 (32.29%), Postives = 129/223 (57.85%), Query Frame = 3

Query:  357 TQKLLEASQLEINELISENADLRRQLLESSNELKTVQNQNKIQERYLNKVNSRDSDVPTIISRYNSELHSLQEQLRRKKEKLDTVERQKTDLDFXXXXXXXXXXXXXXXXXDRQLQEREDLSKKIANLNESLEAEIKKNSDLERSMELLTKKYSYQMALETNKFKELKQSYTETLMELKNIRSSLKEKNKLLEMQNIYSKRVVRAPHR-----LSGKKGKNVT 1010
            T+++L A  L+INEL +  ++LR    E   E + ++     QE+ L++ N  DS++  +++R+N+E H L+E+LRR +E   T ER   + D +L + + +++KL+ L  D+ L ERE+LS+K+      L+    +  +LE++MEL +  +  Q+A E  K  + +Q       E++ + + LKEK + L+ +NIY+ R+ +A  R      S +KG N+T
Sbjct:  155 TKRMLSARLLKINELKNALSELRLHADELQRENRLLRQLQLRQEKALHRYNDTDSEISQLLARHNNETHVLRERLRRSQENQRTAERSLRERDAQLQRCRSQMQKLQQLADDQNLGEREELSRKLIGTQSRLQDSEHRVKELEKNMELSSGSFQRQLANERRKTHDAQQEVKALQEEVERLCTKLKEKERELDARNIYANRMHKASSRKEAENGSKRKGFNIT 377

[Close]

BLAST of SMED30028234 vs. Ensembl Zebrafish
Match: lca5 (lebercilin LCA5 [Source:ZFIN;Acc:ZDB-GENE-090313-268])

HSP 1 Score: 85.5001 bits (210), Expect = 8.471e-17
Identity = 72/223 (32.29%), Postives = 129/223 (57.85%), Query Frame = 3

Query:  357 TQKLLEASQLEINELISENADLRRQLLESSNELKTVQNQNKIQERYLNKVNSRDSDVPTIISRYNSELHSLQEQLRRKKEKLDTVERQKTDLDFXXXXXXXXXXXXXXXXXDRQLQEREDLSKKIANLNESLEAEIKKNSDLERSMELLTKKYSYQMALETNKFKELKQSYTETLMELKNIRSSLKEKNKLLEMQNIYSKRVVRAPHR-----LSGKKGKNVT 1010
            T+++L A  L+INEL +  ++LR    E   E + ++     QE+ L++ N  DS++  +++R+N+E H L+E+LRR +E   T ER   + D +L + + +++KL+ L  D+ L ERE+LS+K+      L+    +  +LE++MEL +  +  Q+A E  K  + +Q       E++ + + LKEK + L+ +NIY+ R+ +A  R      S +KG N+T
Sbjct:  155 TKRMLSARLLKINELKNALSELRLHADELQRENRLLRQLQLRQEKALHRYNDTDSEISQLLARHNNETHVLRERLRRSQENQRTAERSLRERDAQLQRCRSQMQKLQQLADDQNLGEREELSRKLIGTQSRLQDSEHRVKELEKNMELSSGSFQRQLANERRKTHDAQQEVKALQEEVERLCTKLKEKERELDARNIYANRMHKASSRKEAENGSKRKGFNIT 377

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BLAST of SMED30028234 vs. Ensembl Xenopus
Match: atg4d (autophagy related 4D, cysteine peptidase [Source:Xenbase;Acc:XB-GENE-1016717])

HSP 1 Score: 75.8702 bits (185), Expect = 7.762e-14
Identity = 72/233 (30.90%), Postives = 130/233 (55.79%), Query Frame = 3

Query:  309 KKAPFGISNFSKTAES--------TQKLLEASQLEINELISENADLRRQLLESSNELKTVQNQNKI-------QERYLNKVNSRDSDVPTIISRYNSELHSLQEQLRRKKEKLDTVERQKTDLDFXXXXXXXXXXXXXXXXXDRQLQEREDLSKKIANLNESLEAEIKKNSDLERSMELLTKKYSYQMALETNKFKELKQSYTETLMELKNIRSSLKEKNKLLEMQNIYSKRV 962
            K+  +GI + S   ES        T+++L A  L+INEL       R +L +   +LK +QN+NKI       QE+ L K    ++++  +ISR+N+E+ +L+++LR+ +E+   +E++  D + EL +    ++KLK L  +R L ERE+L+KK+  +   L+ + ++  DLE+++EL    +  Q+  E  K  + ++       EL+ +   LKEK + L  +NIY+ R+
Sbjct:   13 KEGKWGIRSQSLNKESPSKDIDLVTKRVLSARLLKINEL-------RNELTDHQTKLKELQNENKILKRLQFRQEKALTKFEDTENEISQLISRHNNEIRTLRDRLRKSQERERNMEKRLKDAEEELYRTNNTLKKLKQLSENRNLAEREELTKKLDIMESKLDEKERRVKDLEKNLELTQSSFQRQLLSEKKKAHDAQEENKTFQEELQRLTQKLKEKERELHAKNIYAYRL 238

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BLAST of SMED30028234 vs. Ensembl Xenopus
Match: atg4d (autophagy related 4D, cysteine peptidase [Source:Xenbase;Acc:XB-GENE-1016717])

HSP 1 Score: 75.8702 bits (185), Expect = 8.223e-14
Identity = 72/233 (30.90%), Postives = 130/233 (55.79%), Query Frame = 3

Query:  309 KKAPFGISNFSKTAES--------TQKLLEASQLEINELISENADLRRQLLESSNELKTVQNQNKI-------QERYLNKVNSRDSDVPTIISRYNSELHSLQEQLRRKKEKLDTVERQKTDLDFXXXXXXXXXXXXXXXXXDRQLQEREDLSKKIANLNESLEAEIKKNSDLERSMELLTKKYSYQMALETNKFKELKQSYTETLMELKNIRSSLKEKNKLLEMQNIYSKRV 962
            K+  +GI + S   ES        T+++L A  L+INEL       R +L +   +LK +QN+NKI       QE+ L K    ++++  +ISR+N+E+ +L+++LR+ +E+   +E++  D + EL +    ++KLK L  +R L ERE+L+KK+  +   L+ + ++  DLE+++EL    +  Q+  E  K  + ++       EL+ +   LKEK + L  +NIY+ R+
Sbjct:   58 KEGKWGIRSQSLNKESPSKDIDLVTKRVLSARLLKINEL-------RNELTDHQTKLKELQNENKILKRLQFRQEKALTKFEDTENEISQLISRHNNEIRTLRDRLRKSQERERNMEKRLKDAEEELYRTNNTLKKLKQLSENRNLAEREELTKKLDIMESKLDEKERRVKDLEKNLELTQSSFQRQLLSEKKKAHDAQEENKTFQEELQRLTQKLKEKERELHAKNIYAYRL 283

[Close]

BLAST of SMED30028234 vs. Ensembl Xenopus
Match: atg4d (autophagy related 4D, cysteine peptidase [Source:Xenbase;Acc:XB-GENE-1016717])

HSP 1 Score: 75.485 bits (184), Expect = 1.064e-13
Identity = 72/233 (30.90%), Postives = 130/233 (55.79%), Query Frame = 3

Query:  309 KKAPFGISNFSKTAES--------TQKLLEASQLEINELISENADLRRQLLESSNELKTVQNQNKI-------QERYLNKVNSRDSDVPTIISRYNSELHSLQEQLRRKKEKLDTVERQKTDLDFXXXXXXXXXXXXXXXXXDRQLQEREDLSKKIANLNESLEAEIKKNSDLERSMELLTKKYSYQMALETNKFKELKQSYTETLMELKNIRSSLKEKNKLLEMQNIYSKRV 962
            K+  +GI + S   ES        T+++L A  L+INEL       R +L +   +LK +QN+NKI       QE+ L K    ++++  +ISR+N+E+ +L+++LR+ +E+   +E++  D + EL +    ++KLK L  +R L ERE+L+KK+  +   L+ + ++  DLE+++EL    +  Q+  E  K  + ++       EL+ +   LKEK + L  +NIY+ R+
Sbjct:   48 KEGKWGIRSQSLNKESPSKDIDLVTKRVLSARLLKINEL-------RNELTDHQTKLKELQNENKILKRLQFRQEKALTKFEDTENEISQLISRHNNEIRTLRDRLRKSQERERNMEKRLKDAEEELYRTNNTLKKLKQLSENRNLAEREELTKKLDIMESKLDEKERRVKDLEKNLELTQSSFQRQLLSEKKKAHDAQEENKTFQEELQRLTQKLKEKERELHAKNIYAYRL 273

[Close]

BLAST of SMED30028234 vs. Ensembl Xenopus
Match: atg4d (autophagy related 4D, cysteine peptidase [Source:Xenbase;Acc:XB-GENE-1016717])

HSP 1 Score: 75.485 bits (184), Expect = 1.225e-13
Identity = 72/233 (30.90%), Postives = 130/233 (55.79%), Query Frame = 3

Query:  309 KKAPFGISNFSKTAES--------TQKLLEASQLEINELISENADLRRQLLESSNELKTVQNQNKI-------QERYLNKVNSRDSDVPTIISRYNSELHSLQEQLRRKKEKLDTVERQKTDLDFXXXXXXXXXXXXXXXXXDRQLQEREDLSKKIANLNESLEAEIKKNSDLERSMELLTKKYSYQMALETNKFKELKQSYTETLMELKNIRSSLKEKNKLLEMQNIYSKRV 962
            K+  +GI + S   ES        T+++L A  L+INEL       R +L +   +LK +QN+NKI       QE+ L K    ++++  +ISR+N+E+ +L+++LR+ +E+   +E++  D + EL +    ++KLK L  +R L ERE+L+KK+  +   L+ + ++  DLE+++EL    +  Q+  E  K  + ++       EL+ +   LKEK + L  +NIY+ R+
Sbjct:   61 KEGKWGIRSQSLNKESPSKDIDLVTKRVLSARLLKINEL-------RNELTDHQTKLKELQNENKILKRLQFRQEKALTKFEDTENEISQLISRHNNEIRTLRDRLRKSQERERNMEKRLKDAEEELYRTNNTLKKLKQLSENRNLAEREELTKKLDIMESKLDEKERRVKDLEKNLELTQSSFQRQLLSEKKKAHDAQEENKTFQEELQRLTQKLKEKERELHAKNIYAYRL 286

[Close]

BLAST of SMED30028234 vs. Ensembl Xenopus
Match: ENSXETT00000020390.1 (lebercilin-like [Source:NCBI gene;Acc:100497171])

HSP 1 Score: 73.1738 bits (178), Expect = 7.061e-13
Identity = 73/242 (30.17%), Postives = 133/242 (54.96%), Query Frame = 3

Query:  282 IASTQNKKIKKAP-------FGISNFSKTAES--------TQKLLEASQLEINELISENADLRRQLLESSNELKTVQNQNKIQERYLNKVNSRDSDVPTIISRYNSELHSLQEQLRRKKEKLDTVERQKTDLDFXXXXXXXXXXXXXXXXXDRQLQEREDLSKKIANLNESLEAEIKKNSDLERSMELLTKKYSYQMALETNKFKELKQSYTETLMELKNIRSSLKEKNKLLEMQNIYSKRV 962
            + ST NK   K P       +G  + S   ES        T+++L A  L+INEL +E  +L+ +L +   E K ++     QE+ LN+    +S++  +ISR+N+E  +L+E LR+ +E+    E++  + + EL +A   ++KLK L  ++ L ERE+L+KK+      L+ + ++  DLE+ +E+    +  Q+ +E  K +  ++     L EL+ +   LKEK + L+++NIY+ R+
Sbjct:   14 LVSTSNKVGSKFPSRKGGAKWGFRSQSLNKESPPKDIALVTKRVLSARLLKINELRNEIRELQIKLEQHQKENKILKKLQFRQEKALNRFEDTESEISQLISRHNNETRALKEHLRKSQERERNTEKRLKEAEDELYRANSTVKKLKQLSENQHLGEREELAKKLDITETKLDEQERRVQDLEKKLEITQNSFQRQLMVEKKKIQNAQEENKMLLEELQKLTYKLKEKERELDVKNIYAYRL 255

[Close]

BLAST of SMED30028234 vs. Ensembl Mouse
Match: Lca5l (Leber congenital amaurosis 5-like [Source:MGI Symbol;Acc:MGI:3041157])

HSP 1 Score: 80.8777 bits (198), Expect = 2.635e-15
Identity = 71/211 (33.65%), Postives = 130/211 (61.61%), Query Frame = 3

Query:  357 TQKLLEASQLEINELISENADLRRQLLESSNELKTVQNQ--NKIQERYLNK----VNSRDSDVPTIISRYNSELHSLQEQLRRKKEKLDTVERQKTDLDFXXXXXXXXXXXXXXXXXDRQLQEREDLSKKIANLNESLEAEIKKNSDLERSMELLTKKYSYQMALETNKFKELKQSYTETL-MELKNIRSSLKEKNKLLEMQNIYSKRVVR 968
            T ++L A   +I EL +E AD+ R+L     E   ++NQ   ++Q R+L      VNS++ ++P I +++ +E+ +L++ LR+ +EK   V R+  + D EL++ K  ++ L+ L  D+ L ERE+L+ ++ +L   +EA  KK  +LE+ + L  + YS Q+A E N+     Q+ T+TL  E++ ++  LKEK++ LE++NIY+ R+++
Sbjct:  140 THRILSARLHKIKELKNELADVHRKL-----EASAIENQFLKQLQLRHLKAIGKYVNSQN-NLPQITAKHQNEVKNLRQLLRKSQEKERAVSRKLRETDGELLRTKDVLQALQRLSEDKNLAEREELTDRLTDLTAKMEANDKKIQNLEKQLRLNNRSYSRQLAKE-NRKTLAAQTATKTLQAEVRQLQQKLKEKDRELEIKNIYTNRILK 343

[Close]

BLAST of SMED30028234 vs. Ensembl Mouse
Match: Lca5l (Leber congenital amaurosis 5-like [Source:MGI Symbol;Acc:MGI:3041157])

HSP 1 Score: 80.8777 bits (198), Expect = 2.708e-15
Identity = 71/211 (33.65%), Postives = 130/211 (61.61%), Query Frame = 3

Query:  357 TQKLLEASQLEINELISENADLRRQLLESSNELKTVQNQ--NKIQERYLNK----VNSRDSDVPTIISRYNSELHSLQEQLRRKKEKLDTVERQKTDLDFXXXXXXXXXXXXXXXXXDRQLQEREDLSKKIANLNESLEAEIKKNSDLERSMELLTKKYSYQMALETNKFKELKQSYTETL-MELKNIRSSLKEKNKLLEMQNIYSKRVVR 968
            T ++L A   +I EL +E AD+ R+L     E   ++NQ   ++Q R+L      VNS++ ++P I +++ +E+ +L++ LR+ +EK   V R+  + D EL++ K  ++ L+ L  D+ L ERE+L+ ++ +L   +EA  KK  +LE+ + L  + YS Q+A E N+     Q+ T+TL  E++ ++  LKEK++ LE++NIY+ R+++
Sbjct:  140 THRILSARLHKIKELKNELADVHRKL-----EASAIENQFLKQLQLRHLKAIGKYVNSQN-NLPQITAKHQNEVKNLRQLLRKSQEKERAVSRKLRETDGELLRTKDVLQALQRLSEDKNLAEREELTDRLTDLTAKMEANDKKIQNLEKQLRLNNRSYSRQLAKE-NRKTLAAQTATKTLQAEVRQLQQKLKEKDRELEIKNIYTNRILK 343

[Close]

BLAST of SMED30028234 vs. Ensembl Mouse
Match: Lca5l (Leber congenital amaurosis 5-like [Source:MGI Symbol;Acc:MGI:3041157])

HSP 1 Score: 80.4925 bits (197), Expect = 2.969e-15
Identity = 71/211 (33.65%), Postives = 130/211 (61.61%), Query Frame = 3

Query:  357 TQKLLEASQLEINELISENADLRRQLLESSNELKTVQNQ--NKIQERYLNK----VNSRDSDVPTIISRYNSELHSLQEQLRRKKEKLDTVERQKTDLDFXXXXXXXXXXXXXXXXXDRQLQEREDLSKKIANLNESLEAEIKKNSDLERSMELLTKKYSYQMALETNKFKELKQSYTETL-MELKNIRSSLKEKNKLLEMQNIYSKRVVR 968
            T ++L A   +I EL +E AD+ R+L     E   ++NQ   ++Q R+L      VNS++ ++P I +++ +E+ +L++ LR+ +EK   V R+  + D EL++ K  ++ L+ L  D+ L ERE+L+ ++ +L   +EA  KK  +LE+ + L  + YS Q+A E N+     Q+ T+TL  E++ ++  LKEK++ LE++NIY+ R+++
Sbjct:  145 THRILSARLHKIKELKNELADVHRKL-----EASAIENQFLKQLQLRHLKAIGKYVNSQN-NLPQITAKHQNEVKNLRQLLRKSQEKERAVSRKLRETDGELLRTKDVLQALQRLSEDKNLAEREELTDRLTDLTAKMEANDKKIQNLEKQLRLNNRSYSRQLAKE-NRKTLAAQTATKTLQAEVRQLQQKLKEKDRELEIKNIYTNRILK 348

[Close]

BLAST of SMED30028234 vs. Ensembl Mouse
Match: Lca5 (Leber congenital amaurosis 5 (human) [Source:MGI Symbol;Acc:MGI:1923032])

HSP 1 Score: 72.7886 bits (177), Expect = 5.627e-13
Identity = 69/211 (32.70%), Postives = 128/211 (60.66%), Query Frame = 3

Query:  357 TQKLLEASQLEINELISENADLR---RQLLESSNELKTVQNQNKIQERYLNKVNSRDSDVPTIISRYNSELHSLQEQLRRKKEKLDTVERQKTDLDFXXXXXXXXXXXXXXXXXDRQLQEREDLSKKIANLNESLEAEIKKNSDLERSMELLTKKYSYQMALETNKFKELKQSYTETLM---ELKNIRSSLKEKNKLLEMQNIYSKRVVRA 971
            T+++L A  L+INEL +E ++L+    QLL+ +  LK++Q +   QE+ LNK    ++++  +I R+N+E+ +L+E+LR+ +EK    E++  + + EL + K  ++KLK +   R L ER+DL+KK+ +    L+   +K  +L +++EL T  +  Q+  E  +  E   +Y E  +   EL+ +   LKEK K L+++NIY+ R+ ++
Sbjct:  104 TKRVLSARLLKINELQNEVSELQVKLAQLLKENKALKSLQYR---QEKALNKFEDAENEISQLIHRHNNEITALKERLRKSQEKERATEKRVKETEGELFRTKFSLQKLKKISEARHLPERDDLAKKLVSAELKLDDTERKIKELSKNLELSTNSFQRQLLAERKRAFE---AYDENKVLQKELQRLHHKLKEKEKELDIKNIYANRLPKS 308

[Close]

BLAST of SMED30028234 vs. Ensembl Mouse
Match: Lca5 (Leber congenital amaurosis 5 (human) [Source:MGI Symbol;Acc:MGI:1923032])

HSP 1 Score: 72.4034 bits (176), Expect = 5.863e-13
Identity = 69/211 (32.70%), Postives = 128/211 (60.66%), Query Frame = 3

Query:  357 TQKLLEASQLEINELISENADLR---RQLLESSNELKTVQNQNKIQERYLNKVNSRDSDVPTIISRYNSELHSLQEQLRRKKEKLDTVERQKTDLDFXXXXXXXXXXXXXXXXXDRQLQEREDLSKKIANLNESLEAEIKKNSDLERSMELLTKKYSYQMALETNKFKELKQSYTETLM---ELKNIRSSLKEKNKLLEMQNIYSKRVVRA 971
            T+++L A  L+INEL +E ++L+    QLL+ +  LK++Q +   QE+ LNK    ++++  +I R+N+E+ +L+E+LR+ +EK    E++  + + EL + K  ++KLK +   R L ER+DL+KK+ +    L+   +K  +L +++EL T  +  Q+  E  +  E   +Y E  +   EL+ +   LKEK K L+++NIY+ R+ ++
Sbjct:  104 TKRVLSARLLKINELQNEVSELQVKLAQLLKENKALKSLQYR---QEKALNKFEDAENEISQLIHRHNNEITALKERLRKSQEKERATEKRVKETEGELFRTKFSLQKLKKISEARHLPERDDLAKKLVSAELKLDDTERKIKELSKNLELSTNSFQRQLLAERKRAFE---AYDENKVLQKELQRLHHKLKEKEKELDIKNIYANRLPKS 308

[Close]

BLAST of SMED30028234 vs. UniProt/SwissProt
Match: sp|Q4R3Q7|LCA5L_MACFA (Lebercilin-like protein OS=Macaca fascicularis OX=9541 GN=LCA5L PE=2 SV=2)

HSP 1 Score: 81.2629 bits (199), Expect = 1.229e-14
Identity = 93/313 (29.71%), Postives = 173/313 (55.27%), Query Frame = 3

Query:  357 TQKLLEASQLEINELISENADLRRQLLESSNELKTVQNQ--NKIQERYLNKVNSRDS---DVPTIISRYNSELHSLQEQLRRKKEKLDTVERQKTDLDFXXXXXXXXXXXXXXXXXDRQLQEREDLSKKIANLNESLEAEIKKNSDLERSMELLTKKYSYQMALETNKFKELKQSYTETL-MELKNIRSSLKEKNKLLEMQNIYSKRVVRAPH------RLSGKKGKNVTLEDSNFPVSKI-------STVPPLKSSLRKEERHNSEHEEASQIK------INLKKKYQVSVKKIENLS----HTAIQHELEN 1208
              ++L A   +I  L +E AD+  +L     E    +NQ   ++Q R+L  +   ++   ++P I++++ +E+ +L++ LR+ +EK  TV R+  + D +L+K K  ++ L+ L  D+ L ERE+L+ K++ +   +EA  KK   LE+ + L ++ +S Q+A+ET K     Q+ T+TL +E+K+++  LKEK++ LE++NIYS R+++  H      ++S    K+V  +  + P + +       S VPPL +  +K   + +  E++++I       +N   K + S  K E+LS    H  +Q  LEN
Sbjct:  140 AHRILSARLHKIKGLKNELADMHHKL-----EAILTENQFLKQLQLRHLKAIGKYENSQNNLPQIMAKHQNEVKNLRQLLRKSQEKERTVSRKLRETDSQLLKTKDTLQALQKLSEDKNLAEREELTHKLSIITTKMEANDKKIQSLEKQLRLNSRVFSRQLAIETRK-TLAAQTATKTLQVEVKHLQQKLKEKDRELEIKNIYSHRILKNLHDTEDYPKVSST--KSVQADRKSLPFTSMRHQGTQKSDVPPLTTKGKKATGNMNHKEKSTEINREIPHCVNKLPKQEDSKTKYEDLSREEKHLEVQVLLEN 444

[Close]

BLAST of SMED30028234 vs. UniProt/SwissProt
Match: sp|Q8C0X0|LCA5L_MOUSE (Lebercilin-like protein OS=Mus musculus OX=10090 GN=Lca5l PE=2 SV=2)

HSP 1 Score: 80.8777 bits (198), Expect = 1.767e-14
Identity = 71/211 (33.65%), Postives = 130/211 (61.61%), Query Frame = 3

Query:  357 TQKLLEASQLEINELISENADLRRQLLESSNELKTVQNQ--NKIQERYLNK----VNSRDSDVPTIISRYNSELHSLQEQLRRKKEKLDTVERQKTDLDFXXXXXXXXXXXXXXXXXDRQLQEREDLSKKIANLNESLEAEIKKNSDLERSMELLTKKYSYQMALETNKFKELKQSYTETL-MELKNIRSSLKEKNKLLEMQNIYSKRVVR 968
            T ++L A   +I EL +E AD+ R+L     E   ++NQ   ++Q R+L      VNS++ ++P I +++ +E+ +L++ LR+ +EK   V R+  + D EL++ K  ++ L+ L  D+ L ERE+L+ ++ +L   +EA  KK  +LE+ + L  + YS Q+A E N+     Q+ T+TL  E++ ++  LKEK++ LE++NIY+ R+++
Sbjct:  140 THRILSARLHKIKELKNELADVHRKL-----EASAIENQFLKQLQLRHLKAIGKYVNSQN-NLPQITAKHQNEVKNLRQLLRKSQEKERAVSRKLRETDGELLRTKDVLQALQRLSEDKNLAEREELTDRLTDLTAKMEANDKKIQNLEKQLRLNNRSYSRQLAKE-NRKTLAAQTATKTLQAEVRQLQQKLKEKDRELEIKNIYTNRILK 343

[Close]

BLAST of SMED30028234 vs. UniProt/SwissProt
Match: sp|B0CM36|LCA5L_PAPAN (Lebercilin-like protein OS=Papio anubis OX=9555 GN=LCA5L PE=3 SV=1)

HSP 1 Score: 78.9518 bits (193), Expect = 5.580e-14
Identity = 69/213 (32.39%), Postives = 130/213 (61.03%), Query Frame = 3

Query:  357 TQKLLEASQLEINELISENADLRRQLLESSNELKTVQNQ--NKIQERYLNKVNSRDS---DVPTIISRYNSELHSLQEQLRRKKEKLDTVERQKTDLDFXXXXXXXXXXXXXXXXXDRQLQEREDLSKKIANLNESLEAEIKKNSDLERSMELLTKKYSYQMALETNKFKELKQSYTETL-MELKNIRSSLKEKNKLLEMQNIYSKRVVRAPH 977
              ++L A   +I  L +E AD+  +L     E    +NQ   ++Q R+L  +   ++   ++P I++++ +E+ +L++ LR+ +EK  TV R+  + D +L+K K  ++ L+ L  D+ L ERE+L+ K++ +   +EA  KK   LE+ + L ++ +S Q+A+ET K     Q+ T+TL +E+K+++  LKEK++ LE++NIYS R+++  H
Sbjct:  140 AHRILSARLHKIKGLKNELADMHHKL-----EAILTENQFLKQLQLRHLKAIGKYENSQNNLPQIMAKHQNEVKNLRQLLRKSQEKERTVSRKLRETDSQLLKTKDTLQALQKLSEDKNLAEREELTHKLSIITTKMEANDKKIQSLEKQLRLNSRAFSRQLAIETRK-TLAAQTATKTLQVEVKHLQQKLKEKDRELEIKNIYSHRILKNLH 346

[Close]

BLAST of SMED30028234 vs. UniProt/SwissProt
Match: sp|O95447|LCA5L_HUMAN (Lebercilin-like protein OS=Homo sapiens OX=9606 GN=LCA5L PE=1 SV=1)

HSP 1 Score: 77.411 bits (189), Expect = 1.831e-13
Identity = 91/313 (29.07%), Postives = 171/313 (54.63%), Query Frame = 3

Query:  357 TQKLLEASQLEINELISENADLRRQLLESSNELKTVQNQ--NKIQERYLNKVNSRDS---DVPTIISRYNSELHSLQEQLRRKKEKLDTVERQKTDLDFXXXXXXXXXXXXXXXXXDRQLQEREDLSKKIANLNESLEAEIKKNSDLERSMELLTKKYSYQMALETNKFKELKQSYTETL-MELKNIRSSLKEKNKLLEMQNIYSKRVVRAPH------RLSGKKGKNVTLEDSNFPVSKI-------STVPPLKSSLRKEERHNSEHEEASQIK------INLKKKYQVSVKKIENLS----HTAIQHELEN 1208
              ++L A   +I  L +E AD+  +L     E    +NQ   ++Q R+L  +   ++   ++P I++++ +E+ +L++ LR+ +EK  T+ R+  + D +L+K K  ++ L+ L  D+ L ERE+L+ K++ +   ++A  KK   LE+ + L  + +S Q+A+ET K     Q+ T+TL +E+K+++  LKEK++ LE++NIYS R+++  H      ++S    K+V  +    P + +       S VPPL +  +K   +    E++++I       +N   K + S +K E+LS    H  +Q  LEN
Sbjct:  140 AHRILSARLHKIKGLKNELADMHHKL-----EAILTENQFLKQLQLRHLKAIGKYENSQNNLPQIMAKHQNEVKNLRQLLRKSQEKERTLSRKLRETDSQLLKTKDILQALQKLSEDKNLAEREELTHKLSIITTKMDANDKKIQSLEKQLRLNCRAFSRQLAIETRK-TLAAQTATKTLQVEVKHLQQKLKEKDRELEIKNIYSHRILKNLHDTEDYPKVSST--KSVQADRKILPFTSMRHQGTQKSDVPPLTTKGKKATGNIDHKEKSTEINHEIPHCVNKLPKQEDSKRKYEDLSGEEKHLEVQILLEN 444

[Close]

BLAST of SMED30028234 vs. UniProt/SwissProt
Match: sp|A7E3D8|LCA5L_BOVIN (Lebercilin-like protein OS=Bos taurus OX=9913 GN=LCA5L PE=2 SV=2)

HSP 1 Score: 76.2554 bits (186), Expect = 4.919e-13
Identity = 69/226 (30.53%), Postives = 131/226 (57.96%), Query Frame = 3

Query:  318 PFGISNFSKTAES----TQKLLEASQLEINELISENADLRRQLLESSNELKTVQNQ--NKIQERYLNKVNSRDS---DVPTIISRYNSELHSLQEQLRRKKEKLDTVERQKTDLDFXXXXXXXXXXXXXXXXXDRQLQEREDLSKKIANLNESLEAEIKKNSDLERSMELLTKKYSYQMALETNKFKELKQSYTETL-MELKNIRSSLKEKNKLLEMQNIYSKRVV 965
            PF  S  S  A+       ++L A   +I EL +E ADL  +      E   ++NQ   ++Q ++L  +   ++   ++P I++++ +E+ +L++ LR+ + K     R+  ++D EL++ K  ++ L+ +  +  L ERE+L+++++ L   +EA  KK  DLE+ + L  K ++ Q+  E N+     Q+ T+TL ME+K+++  LKEK++ LE++NIYS R+V
Sbjct:  122 PFLNSQISVAAQRRDAMIHRILSARLHKIKELKNELADLHHKA-----EATVIENQFLKQVQLKHLKAIGKYENSQNNLPQIMAKHQNEVKNLRQLLRKSQGKERATSRKLREMDGELLRTKDALQALQKISEENNLPEREELTQRLSILTAKMEANDKKIQDLEKQLRLNNKAFTRQLTFE-NRKTLAAQAATKTLQMEIKHLQQKLKEKDRELEIRNIYSNRIV 341

[Close]

BLAST of SMED30028234 vs. TrEMBL
Match: A0A1S3IZ48 (lebercilin isoform X5 OS=Lingula unguis OX=7574 GN=LOC106168658 PE=4 SV=1)

HSP 1 Score: 112.079 bits (279), Expect = 8.309e-22
Identity = 79/240 (32.92%), Postives = 147/240 (61.25%), Query Frame = 3

Query:  267 KPAFHIASTQNKKIKKAPFGISNFSKTAES-TQKLLEASQLEINELISENADLRRQLLESSNELKTVQNQNKIQERYLNKVNSRDSDVPTIISRYNSELHSLQEQLRRKKEKLDTVERQKTDLDFXXXXXXXXXXXXXXXXXDRQLQEREDLSKKIANLNESLEAEIKKNSDLERSMELLTKKYSYQMALETNKFKELKQSYTETLMELKNIRSSLKEKNKLLEMQNIYSKRVVRAPHRL 983
            KP  H+A    K   +A  G       +++ T+++L A +L+I+E+ +E  D+ +Q+ +   E KT++     Q+R L +   ++SD+P +I  +  E+ +L+EQ+++ KEK    E++  + + ++ K KK+++KL+ L  D++L ER+DL++K+A     LE + K+  DLE+ +E+L K   +++A+E NK KE ++   E     + ++ S +EK K LE++NIYS R+V+ PH+L
Sbjct:   12 KPGAHLAQLAQKSYSRATRGGRTSKAVSDNVTRRVLSARKLKISEMRNEIEDMHQQIADLKLENKTLKRLQAKQDRELQRFEDKESDLPQLIKGHADEVRTLREQIKKLKEKSSRTEKKLREAEDDVTKTKKQLKKLEDLSHDKKLGERDDLNRKLAKAENELEEKTKRCQDLEKHVEILKKAQRHEVAVEINKHKETQKQLLEMRDMYERLKESYREKEKELEIKNIYSNRIVKPPHKL 251

[Close]

BLAST of SMED30028234 vs. TrEMBL
Match: A0A267F5R7 (Lebercilin domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig015080g2 PE=4 SV=1)

HSP 1 Score: 108.612 bits (270), Expect = 1.125e-20
Identity = 77/213 (36.15%), Postives = 126/213 (59.15%), Query Frame = 3

Query:  369 LEASQLEINELISENADLRRQLLESSNELKTVQNQNKIQERYLNKVNSRDSDVPTIISRYNSELHSLQEQLRRKKEKLDTVERQKTDLDFXXXXXXXXXXXXXXXXXDRQLQEREDLSKKIANL-NESLEAEIKKNSDLERSMELLTKKYSYQMALETNKFKELKQSYTETLMELKNIRSSLKEKNKLLEMQNIYSKRVVRAPHRLSGKKGKN 1004
            LE +  E+NEL +EN              + +Q QN+I ER LNK ++ DS +   I+R+  E+ +L+EQLRR K++      +  DL+ E  + KK  ++L+   +   L +RE L+K+++ L N++ E E KK  DLER ME L K + +++A+E ++ KE ++   E   +L+   ++LKEK K+L++QNIY++R VR PH L+   G  
Sbjct:  175 LEFAMKELNELRTEN--------------RLLQKQNRINERALNKYDNEDSQLSQTIARHQEEVRTLKEQLRRYKDRCSEAGARNKDLEAENARLKKFNDRLREQASQEGLPDREALAKRVSELENDNAEKE-KKIQDLERHMEHLQKNHRHEVAIEVHRHKETQRKVRELEEQLQEATTALKEKEKILDIQNIYAQRAVRPPHHLNSSAGGG 372

[Close]

BLAST of SMED30028234 vs. TrEMBL
Match: A0A1I8GEC1 (Lebercilin domain-containing protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 108.227 bits (269), Expect = 1.380e-20
Identity = 77/213 (36.15%), Postives = 126/213 (59.15%), Query Frame = 3

Query:  369 LEASQLEINELISENADLRRQLLESSNELKTVQNQNKIQERYLNKVNSRDSDVPTIISRYNSELHSLQEQLRRKKEKLDTVERQKTDLDFXXXXXXXXXXXXXXXXXDRQLQEREDLSKKIANL-NESLEAEIKKNSDLERSMELLTKKYSYQMALETNKFKELKQSYTETLMELKNIRSSLKEKNKLLEMQNIYSKRVVRAPHRLSGKKGKN 1004
            LE +  E+NEL +EN              + +Q QN+I ER LNK ++ DS +   I+R+  E+ +L+EQLRR K++      +  DL+ E  + KK  ++L+   +   L +RE L+K+++ L N++ E E KK  DLER ME L K + +++A+E ++ KE ++   E   +L+   ++LKEK K+L++QNIY++R VR PH L+   G  
Sbjct:  176 LEFAMKELNELRTEN--------------RLLQKQNRINERALNKYDNEDSQLSQTIARHQEEVRTLKEQLRRYKDRCSEAGARNKDLEAENARLKKFNDRLREQASQEGLPDREALAKRVSELENDNAEKE-KKIQDLERHMEHLQKNHRHEVAIEVHRHKETQRKVRELEEQLQEATTALKEKEKILDIQNIYAQRAVRPPHHLNSSAGGG 373

[Close]

BLAST of SMED30028234 vs. TrEMBL
Match: A0A1I8G3D1 (Lebercilin domain-containing protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 106.686 bits (265), Expect = 4.050e-20
Identity = 76/213 (35.68%), Postives = 126/213 (59.15%), Query Frame = 3

Query:  369 LEASQLEINELISENADLRRQLLESSNELKTVQNQNKIQERYLNKVNSRDSDVPTIISRYNSELHSLQEQLRRKKEKLDTVERQKTDLDFXXXXXXXXXXXXXXXXXDRQLQEREDLSKKIANL-NESLEAEIKKNSDLERSMELLTKKYSYQMALETNKFKELKQSYTETLMELKNIRSSLKEKNKLLEMQNIYSKRVVRAPHRLSGKKGKN 1004
            LE +  E+NEL +EN              + +Q QN+I ER LNK ++ DS +   I+R+  E+ +L+EQLRR K++      +  DL+ E  + KK  ++L+   +   L +RE L+K+++ L N++ E E KK  DLER +E L K + +++A+E ++ KE ++   E   +L+   ++LKEK K+L++QNIY++R VR PH L+   G  
Sbjct:  175 LEFAMKELNELRTEN--------------RLLQKQNRINERALNKYDNEDSQLSQTIARHQEEVRTLKEQLRRYKDRCSEAGARNKDLEAENARLKKFNDRLREQASQEGLPDREALAKRVSELENDNAEKE-KKIQDLERHLEHLQKNHRHEVAIEVHRHKETQRKVRELEEQLQEATTALKEKEKILDIQNIYAQRAVRPPHHLNSSAGGG 372

[Close]

BLAST of SMED30028234 vs. TrEMBL
Match: A0A267F3R2 (Lebercilin domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig015184g1 PE=4 SV=1)

HSP 1 Score: 106.686 bits (265), Expect = 4.421e-20
Identity = 76/213 (35.68%), Postives = 126/213 (59.15%), Query Frame = 3

Query:  369 LEASQLEINELISENADLRRQLLESSNELKTVQNQNKIQERYLNKVNSRDSDVPTIISRYNSELHSLQEQLRRKKEKLDTVERQKTDLDFXXXXXXXXXXXXXXXXXDRQLQEREDLSKKIANL-NESLEAEIKKNSDLERSMELLTKKYSYQMALETNKFKELKQSYTETLMELKNIRSSLKEKNKLLEMQNIYSKRVVRAPHRLSGKKGKN 1004
            LE +  E+NEL +EN              + +Q QN+I ER LNK ++ DS +   I+R+  E+ +L+EQLRR K++      +  DL+ E  + KK  ++L+   +   L +RE L+K+++ L N++ E E KK  DLER +E L K + +++A+E ++ KE ++   E   +L+   ++LKEK K+L++QNIY++R VR PH L+   G  
Sbjct:  175 LEFAMKELNELRTEN--------------RLLQKQNRINERALNKYDNEDSQLSQTIARHQEEVRTLKEQLRRYKDRCSEAGARNKDLEAENARLKKFNDRLREQASQEGLPDREALAKRVSELENDNAEKE-KKIQDLERHLEHLQKNHRHEVAIEVHRHKETQRKVRELEEQLQEATTALKEKEKILDIQNIYTQRAVRPPHHLNSSAGGG 372

[Close]

BLAST of SMED30028234 vs. Ensembl Cavefish
Match: lca5 (lebercilin LCA5 [Source:NCBI gene;Acc:103033771])

HSP 1 Score: 78.1814 bits (191), Expect = 1.465e-14
Identity = 63/205 (30.73%), Postives = 118/205 (57.56%), Query Frame = 3

Query:  357 TQKLLEASQLEINELISENADLRRQLLESSNELKTVQNQNKIQERYLNKVNSRDSDVPTIISRYNSELHSLQEQLRRKKEKLDTVERQKTDLDFXXXXXXXXXXXXXXXXXDRQLQEREDLSKKIANLNESLEAEIKKNSDLERSMELLTKKYSYQMALETNKFKELKQSYTETLMELKNIRSSLKEKNKLLEMQNIYSKRVVRA 971
            T++LL A  L+I+EL +  ++LR    E   E + ++      E+ L+K +  +S++  +ISR+N+E H L+E+LRR +E+  T ER   + D +L + + +++KL+ L  D+ L ER++LS+K+ +     +   ++  +LE+++EL    +  Q+A E  K  +  Q       E + + + LK K + L+ +NIY+ R+ RA
Sbjct:  166 TKRLLSARLLKISELKNALSELRTHADELQRENRLLRQLQLRHEKALHKYSDTESEISQLISRHNNETHVLRERLRRSQERERTAERSYREADAQLQRCRSQLQKLQQLADDQNLGERDELSRKLTHAQARAQESDRRVKELEKNLELSKGSFQRQLAGERRKTHDAMQEIQALREETEKLSAILKGKERELDARNIYANRITRA 370

[Close]

Query:  357 TQKLLEASQLEINELISENADLRRQLLESSNELKTVQNQNKIQERYLNKVNSRDSDVPTIISRYNSELHSLQEQLRRKKEKLDTVERQKTDLDFXXXXXXXXXXXXXXXXXDRQLQEREDLSKKIANLNESLEAEIKKNSDLERSMELLTKKYSYQMALETNKFKELKQSYTETLMELKNIRSSLKEKNKLLEMQNIYSKRVVRA 971
            T++LL A  L+I+EL +  ++LR    E   E + ++      E+ L+K +  +S++  +ISR+N+E H L+E+LRR +E+  T ER   + D +L + + +++KL+ L  D+ L ER++LS+K+ +     +   ++  +LE+++EL    +  Q+A E  K  +  Q       E + + + LK K + L+ +NIY+ R+ RA
Sbjct:  166 TKRLLSARLLKISELKNALSELRTHADELQRENRLLRQLQLRHEKALHKYSDTESEISQLISRHNNETHVLRERLRRSQERERTAERSYREADAQLQRCRSQLQKLQQLADDQNLGERDELSRKLTHAQARAQESDRRVKELEKNLELSKGSFQRQLAGERRKTHDAMQEIQALREETEKLSAILKGKERELDARNIYANRITRA 370

[Close]

BLAST of SMED30028234 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000010615.1 (pep scaffold:Pmarinus_7.0:GL477304:118413:123523:-1 gene:ENSPMAG00000009611.1 transcript:ENSPMAT00000010615.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 69.3218 bits (168), Expect = 1.315e-13
Identity = 56/194 (28.87%), Postives = 111/194 (57.22%), Query Frame = 3

Query:  399 LISENADLRRQLLESSNELKTVQNQNKIQERYLNKVNSRDSDVPTIISRYNSELHSLQEQLRRKKEKLDTVERQKTDLDFXXXXXXXXXXXXXXXXXDRQLQEREDLSKKIANLNESLEAEIKKNSDLERSMELLTKKYSYQMALETNKFKELKQSYTETLMELKNIRSSLKEKNKLLEMQNIYSKRVVRAPHR 980
            L +E ++L  +L E++ E + ++     QE+ L +  S++ D+P +++R+ +E  +L+E LRR +E      R+      EL   ++ +++L+ L   R L ERE+L++ +A     L+   ++  DLE++++L T  Y  Q+A E  K +E +   T    E++ +   LK+K + L+++NIY+ R+++A  R
Sbjct:   19 LRNEVSELEARLDEATRENRALKQLQHRQEKALARFESQEGDLPQLLARHAAETRALREGLRRAREAERDAARRLRAAQDELATTREGLQRLQRLSEQRGLGEREELARSLARAETRLDESERRVKDLEKNLDLTTNSYQRQLAAERRKTQEAQAQVTALQEEIERLTQKLKDKERELDVKNIYASRLLKASPR 212

[Close]

BLAST of SMED30028234 vs. Ensembl Medaka
Match: lca5 (lebercilin LCA5 [Source:NCBI gene;Acc:101164200])

HSP 1 Score: 64.3142 bits (155), Expect = 2.840e-10
Identity = 63/206 (30.58%), Postives = 121/206 (58.74%), Query Frame = 3

Query:  357 TQKLLEASQLEINELISENADLRRQLLESSNELKTVQNQNKIQERYLNKVNSRDSDVPTIISRYNSELHSLQEQLRRKKEKLDTVERQKTDLDFXXXXXXXXXXXXXXXXXDRQLQEREDLSKKIANLNESLEAEIKKNSDLERSMELLTKKYSYQMALETNKFKELKQSYTETLM-ELKNIRSSLKEKNKLLEMQNIYSKRVVRA 971
            T+++L A  L+INEL +   +L++++ +   E + ++     QE+ L   +  +S++  ++SR+++E+H L+E+LRR +E+  + E++  D + +L K++  I +LK LV  R+L  R++LS+++       +   +K  DLER++EL    +  Q+  E  K     Q    TL  EL+ + + LKEK + L+ +NIY+ R+ +A
Sbjct:  173 TKRMLSARLLKINELRNALTELQQRMEDLKKENRVLKQLQVRQEKALRHYDDTESEISLLLSRHSNEIHVLRERLRRTQERERSSEQRLKDAEEQLQKSRATIARLKKLVDQRELGTRDELSRRLDEEKIRTQEAQQKIKDLERNLELTNSSFQRQIVSEKKKTV-CAQEEVRTLQEELQRLTNKLKEKERDLDAKNIYANRLAKA 377

[Close]

Query:  357 TQKLLEASQLEINELISENADLRRQLLESSNELKTVQNQNKIQERYLNKVNSRDSDVPTIISRYNSELHSLQEQLRRKKEKLDTVERQKTDLDFXXXXXXXXXXXXXXXXXDRQLQEREDLSKKIANLNESLEAEIKKNSDLERSMELLTKKYSYQMALETNKFKELKQSYTETLM-ELKNIRSSLKEKNKLLEMQNIYSKRVVRA 971
            T+++L A  L+INEL +   +L++++ +   E + ++     QE+ L   +  +S++  ++SR+++E+H L+E+LRR +E+  + E++  D + +L K++  I +LK LV  R+L  R++LS+++       +   +K  DLER++EL    +  Q+  E  K     Q    TL  EL+ + + LKEK + L+ +NIY+ R+ +A
Sbjct:  173 TKRMLSARLLKINELRNALTELQQRMEDLKKENRVLKQLQVRQEKALRHYDDTESEISLLLSRHSNEIHVLRERLRRTQERERSSEQRLKDAEEQLQKSRATIARLKKLVDQRELGTRDELSRRLDEEKIRTQEAQQKIKDLERNLELTNSSFQRQIVSEKKKTV-CAQEEVRTLQEELQRLTNKLKEKERDLDAKNIYANRLAKA 377

[Close]

BLAST of SMED30028234 vs. Planmine SMEST
Match: SMESG000004781.1 (SMESG000004781.1)

HSP 1 Score: 928.317 bits (2398), Expect = 0.000e+0
Identity = 558/567 (98.41%), Postives = 559/567 (98.59%), Query Frame = 3

Query:   42 MNIKKENDFVIKVKTPTTLISKHISLSEKTNRRSVXXXXXXXXXXXXXXXXXXXXXXXXXXPVKSSAVHRVKRISKPAFHIASTQNKKIKKAPFGISNFSKTAESTQKLLEASQLEINELISENADLRRQLLESSNELKTVQNQNKIQERYLNKVNSRDSDVPTIISRYNSELHSLQEQLRRKKEKLDTVERQKTDLDFXXXXXXXXXXXXXXXXXDRQLQEREDLSKKIANLNESLEAEIKKNSDLERSMELLTKKYSYQMALETNKFKELKQSYTETLMELKNIRSSLKEKNKLLEMQNIYSKRVVRAPHRLSGKKGKNVTLEDSNFPVSKISTVPPLKSSLRKEERHNSEHEEASQIKINLKKKYQVSVKKIENLSHTAIQHELENEKKSEDNKKPTRVLFYGDTPSEISEIEEKSDTKFSDIFETVKRNMQTEIKVDTNNNSNKEDAQKSELLAKLKQIDDNKKKENLKSEMSNLDLVDVELKTIMKNEKESDSDDLDMFXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPCLLVPRRNAKLTTSKMPQNKNSKRETTQFEF 1742
            MNIKKENDFVIKVKTPTTLISKHISLSEKTNRRSVSQASKSSKNNRSSQSLSYSDSFDSDSPVKSSAVHRVKRISKPAFHIASTQNKKIKKAPFGISNFSKTAESTQKLLEASQLEINELISENADLRRQLLESSNELKTVQNQNKIQERYLNKVNSRDSDVPTIISRYNSELHSLQEQLRRKKEKLDTVERQKTDLDFELVKAKKKIEKLKALVADRQLQEREDLSKKIANLNESLEAEIKKNSDLERSMELLTKKYSYQMALETNKFKELKQSYTETLMELKNIRSSLKEKNKLLEMQNIYSKRVVRAPHRLSGKKGKNVTLEDSNFPVSKISTVPPLKSSLRKEERHNSEHEEASQIKINLKKKYQVSVKKIENLSHTAIQHELENEKKSEDNKKPTRVLFYGDTPSEISEIEEKSDTKFSDIFETVKRNMQTEIKVDTNNNSNKEDAQKSELLAKLKQIDDNKKKENLKSEMSNLDLVDVELKTIMKNEKESDSDDLDMFSTSGSSVTGDSDEDDDDEEEESSDKKSDSSSPCLLVPRRNAKLTTSKMPQNK  K +     F
Sbjct:    1 MNIKKENDFVIKVKTPTTLISKHISLSEKTNRRSVSQASKSSKNNRSSQSLSYSDSFDSDSPVKSSAVHRVKRISKPAFHIASTQNKKIKKAPFGISNFSKTAESTQKLLEASQLEINELISENADLRRQLLESSNELKTVQNQNKIQERYLNKVNSRDSDVPTIISRYNSELHSLQEQLRRKKEKLDTVERQKTDLDFELVKAKKKIEKLKALVADRQLQEREDLSKKIANLNESLEAEIKKNSDLERSMELLTKKYSYQMALETNKFKELKQSYTETLMELKNIRSSLKEKNKLLEMQNIYSKRVVRAPHRLSGKKGKNVTLEDSNFPVSKISTVPPLKSSLRKEERHNSEHEEASQIKINLKKKYQVSVKKIENLSHTAIQHELENEKKSEDNKKPTRVLFYGDTPSEISEIEEKSDTKFSDIFETVKRNMQTEIKVDTNNNSNKEDAQKSELLAKLKQIDDNKKKENLKSEMSNLDLVDVELKTIMKNEKESDSDDLDMFSTSGSSVTGDSDEDDDDEEEESSDKKSDSSSPCLLVPRRNAKLTTSKMPQNKTVKEKQRNLNF 567

[Close]

BLAST of SMED30028234 vs. Planmine SMEST
Match: SMESG000022949.1 (SMESG000022949.1)

HSP 1 Score: 81.6481 bits (200), Expect = 7.627e-16
Identity = 83/260 (31.92%), Postives = 147/260 (56.54%), Query Frame = 3

Query:  225 PVKSSAVHR-VKRISKPAFHIASTQNKKI-KKAPFGISNFSKTAESTQKLLEASQLEI---NELISENADLRRQLLESSNELKTVQNQNKIQERYLNKVNSRDSDVPTIISRYNSELHSLQEQLRRKKEKLDTVERQKTDLDFXXXXXXXXXXXXXXXXXDRQLQEREDLSKKIANLNESLEAEIKKNSDLERSMELLTKKYSYQMALETNKFKELKQSYTETLMELKNIRSSLKEKNKLLEMQNIYSKRVVRAPHRLSG 989
            P K+  ++R  K++S  A +     N+K+  +  F  S    TA   Q++L A + +I   N LISE   L++Q+ +   E KT+++Q   Q++ L+K++SR SD+P I+ ++ +E+ +L+EQL+R K+ L+   +Q+ + D E +K +  + K   L+ ++ L ER  L  ++    E LE+  K      + +E L K +  ++     + K+ +    E+  EL  ++ +LKEK KLLE+QNIYS R ++ PHR+ G
Sbjct:   38 PRKTEHIYRNFKQVSGTAKNRQRNNNRKMMPQKRFTESQLEVTA---QRILSARKHKITDQNNLISE---LQKQIDDLLVENKTLKSQTYRQDKALSKIDSRQSDIPKILEQHQAEVTNLKEQLKRTKDLLNNEMKQRKESDSEALKLRNSLTKANQLIFEKNLGERNILQLQLVETQEKLESSEKSLEVTSKHLENLEKSHKREIYNLRKQLKQYEVQCRESEKELDLVKINLKEKEKLLEIQNIYSHR-IKMPHRIPG 290

[Close]

BLAST of SMED30028234 vs. Planmine SMEST
Match: SMESG000022949.1 (SMESG000022949.1)

HSP 1 Score: 80.1073 bits (196), Expect = 2.317e-15
Identity = 71/215 (33.02%), Postives = 127/215 (59.07%), Query Frame = 3

Query:  354 STQKLLEASQLEI---NELISENADLRRQLLESSNELKTVQNQNKIQERYLNKVNSRDSDVPTIISRYNSELHSLQEQLRRKKEKLDTVERQKTDLDFXXXXXXXXXXXXXXXXXDRQLQEREDLSKKIANLNESLEAEIKKNSDLERSMELLTKKYSYQMALETNKFKELKQSYTETLMELKNIRSSLKEKNKLLEMQNIYSKRVVRAPHRLSG 989
            + Q++L A + +I   N LISE   L++Q+ +   E KT+++Q   Q++ L+K++SR SD+P I+ ++ +E+ +L+EQL+R K+ L+   +Q+ + D E +K +  + K   L+ ++ L ER  L  ++    E LE+  K      + +E L K +  ++     + K+ +    E+  EL  ++ +LKEK KLLE+QNIYS R ++ PHR+ G
Sbjct:  116 TAQRILSARKHKITDQNNLISE---LQKQIDDLLVENKTLKSQTYRQDKALSKIDSRQSDIPKILEQHQAEVTNLKEQLKRTKDLLNNEMKQRKESDSEALKLRNSLTKANQLIFEKNLGERNILQLQLVETQEKLESSEKSLEVTSKHLENLEKSHKREIYNLRKQLKQYEVQCRESEKELDLVKINLKEKEKLLEIQNIYSHR-IKMPHRIPG 326

The following BLAST results are available for this feature:

BLAST of SMED30028234 vs. Ensembl Human
BLAST of SMED30028234 vs. Ensembl Celegans
BLAST of SMED30028234 vs. Ensembl Fly
BLAST of SMED30028234 vs. Ensembl Zebrafish
BLAST of SMED30028234 vs. Ensembl Xenopus
BLAST of SMED30028234 vs. Ensembl Mouse
BLAST of SMED30028234 vs. UniProt/SwissProt
BLAST of SMED30028234 vs. TrEMBL
BLAST of SMED30028234 vs. Ensembl Cavefish
BLAST of SMED30028234 vs. Ensembl Sea Lamprey
BLAST of SMED30028234 vs. Ensembl Yeast
BLAST of SMED30028234 vs. Ensembl Nematostella
BLAST of SMED30028234 vs. Ensembl Medaka
BLAST of SMED30028234 vs. Planmine SMEST

BLAST of SMED30028234 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
LCA5L	3.813e-14	29.07	lebercilin LCA5 like [Source:HGNC Symbol;Acc:HGNC:...	[more]
LCA5L	3.813e-14	29.07	lebercilin LCA5 like [Source:HGNC Symbol;Acc:HGNC:...	[more]
LCA5L	3.813e-14	29.07	lebercilin LCA5 like [Source:HGNC Symbol;Acc:HGNC:...	[more]
LCA5	2.816e-12	31.22	lebercilin LCA5 [Source:HGNC Symbol;Acc:HGNC:31923...	[more]
LCA5	3.632e-12	31.22	lebercilin LCA5 [Source:HGNC Symbol;Acc:HGNC:31923...	[more]

BLAST of SMED30028234 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30028234 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30028234 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 3


Match Name	E-value	Identity	Description
lca5	7.836e-17	32.29	lebercilin LCA5 [Source:ZFIN;Acc:ZDB-GENE-090313-2...	[more]
lca5	8.400e-17	32.29	lebercilin LCA5 [Source:ZFIN;Acc:ZDB-GENE-090313-2...	[more]
lca5	8.471e-17	32.29	lebercilin LCA5 [Source:ZFIN;Acc:ZDB-GENE-090313-2...	[more]

BLAST of SMED30028234 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5


Match Name	E-value	Identity	Description
atg4d	7.762e-14	30.90	autophagy related 4D, cysteine peptidase [Source:X...	[more]
atg4d	8.223e-14	30.90	autophagy related 4D, cysteine peptidase [Source:X...	[more]
atg4d	1.064e-13	30.90	autophagy related 4D, cysteine peptidase [Source:X...	[more]
atg4d	1.225e-13	30.90	autophagy related 4D, cysteine peptidase [Source:X...	[more]
ENSXETT00000020390.1	7.061e-13	30.17	lebercilin-like [Source:NCBI gene;Acc:100497171]	[more]

BLAST of SMED30028234 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Lca5l	2.635e-15	33.65	Leber congenital amaurosis 5-like [Source:MGI Symb...	[more]
Lca5l	2.708e-15	33.65	Leber congenital amaurosis 5-like [Source:MGI Symb...	[more]
Lca5l	2.969e-15	33.65	Leber congenital amaurosis 5-like [Source:MGI Symb...	[more]
Lca5	5.627e-13	32.70	Leber congenital amaurosis 5 (human) [Source:MGI S...	[more]
Lca5	5.863e-13	32.70	Leber congenital amaurosis 5 (human) [Source:MGI S...	[more]

BLAST of SMED30028234 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|Q4R3Q7\|LCA5L_MACFA	1.229e-14	29.71	Lebercilin-like protein OS=Macaca fascicularis OX=...	[more]
sp\|Q8C0X0\|LCA5L_MOUSE	1.767e-14	33.65	Lebercilin-like protein OS=Mus musculus OX=10090 G...	[more]
sp\|B0CM36\|LCA5L_PAPAN	5.580e-14	32.39	Lebercilin-like protein OS=Papio anubis OX=9555 GN...	[more]
sp\|O95447\|LCA5L_HUMAN	1.831e-13	29.07	Lebercilin-like protein OS=Homo sapiens OX=9606 GN...	[more]
sp\|A7E3D8\|LCA5L_BOVIN	4.919e-13	30.53	Lebercilin-like protein OS=Bos taurus OX=9913 GN=L...	[more]

BLAST of SMED30028234 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A1S3IZ48	8.309e-22	32.92	lebercilin isoform X5 OS=Lingula unguis OX=7574 GN...	[more]
A0A267F5R7	1.125e-20	36.15	Lebercilin domain-containing protein OS=Macrostomu...	[more]
A0A1I8GEC1	1.380e-20	36.15	Lebercilin domain-containing protein OS=Macrostomu...	[more]
A0A1I8G3D1	4.050e-20	35.68	Lebercilin domain-containing protein OS=Macrostomu...	[more]
A0A267F3R2	4.421e-20	35.68	Lebercilin domain-containing protein OS=Macrostomu...	[more]

BLAST of SMED30028234 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2


Match Name	E-value	Identity	Description
lca5	1.465e-14	30.73	lebercilin LCA5 [Source:NCBI gene;Acc:103033771]	[more]
lca5	1.465e-14	30.73	lebercilin LCA5 [Source:NCBI gene;Acc:103033771]	[more]

BLAST of SMED30028234 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1


Match Name	E-value	Identity	Description
ENSPMAT00000010615.1	1.315e-13	28.87	pep scaffold:Pmarinus_7.0:GL477304:118413:123523:-...	[more]

BLAST of SMED30028234 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30028234 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30028234 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2


Match Name	E-value	Identity	Description
lca5	2.840e-10	30.58	lebercilin LCA5 [Source:NCBI gene;Acc:101164200]	[more]
lca5	2.840e-10	30.58	lebercilin LCA5 [Source:NCBI gene;Acc:101164200]	[more]

BLAST of SMED30028234 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3


Match Name	E-value	Identity	Description
SMESG000004781.1	0.000e+0	98.41	SMESG000004781.1	[more]
SMESG000022949.1	7.627e-16	31.92	SMESG000022949.1	[more]
SMESG000022949.1	2.317e-15	33.02	SMESG000022949.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30028234 ID=SMED30028234|Name=SMED30028234|organism=Schmidtea mediterranea sexual|type=transcript|length=1963bp
TCTAATTCAAAAAATATAAAATTTTTCTTTTCGAATAGGAAATGAATATA
AAAAAAGAAAATGATTTTGTTATTAAAGTCAAAACTCCAACTACTTTAAT
ATCAAAACATATTTCTTTAAGTGAGAAAACAAATCGCAGATCTGTATCAC
AGGCATCGAAAAGTTCCAAAAATAATAGATCCTCTCAATCACTTTCATAT
AGTGATTCATTTGACAGTGATTCTCCGGTTAAAAGTTCCGCCGTTCATAG
AGTCAAGCGAATTTCAAAGCCGGCTTTTCATATCGCATCAACTCAAAACA
AAAAAATTAAAAAAGCACCTTTTGGAATTTCTAATTTCTCGAAAACTGCC
GAGAGTACCCAGAAATTATTGGAGGCCAGTCAATTGGAAATTAATGAGTT
AATTAGTGAAAATGCAGATTTGCGTCGACAATTACTCGAAAGTTCAAATG
AACTGAAAACAGTTCAGAATCAAAATAAAATCCAAGAAAGATATTTAAAT
AAAGTAAACAGTCGTGACTCAGATGTCCCAACCATAATTTCAAGGTACAA
TTCAGAATTGCATTCATTGCAAGAGCAACTGAGACGTAAAAAGGAAAAAT
TAGATACGGTGGAAAGACAAAAAACAGATTTAGATTTTGAATTAGTTAAA
GCAAAGAAAAAAATTGAAAAACTTAAAGCTTTAGTAGCTGATAGACAATT
ACAAGAAAGGGAAGATTTATCAAAAAAAATTGCAAATCTGAATGAGTCTT
TGGAAGCTGAAATAAAAAAGAATTCAGATTTAGAAAGAAGTATGGAGCTC
CTAACAAAAAAATACAGTTATCAAATGGCTCTTGAAACAAATAAGTTTAA
GGAATTAAAGCAGTCTTATACTGAGACACTAATGGAATTGAAAAATATCC
GAAGTTCCTTAAAAGAAAAAAACAAACTGTTAGAAATGCAGAATATTTAT
AGCAAACGAGTTGTTAGAGCACCTCATAGGTTATCTGGCAAAAAGGGAAA
AAATGTCACATTAGAAGATTCTAATTTTCCAGTGTCAAAAATATCAACAG
TGCCTCCTTTGAAATCTTCTCTAAGAAAAGAGGAAAGGCATAATAGTGAA
CATGAAGAAGCATCTCAAATAAAAATTAACTTAAAAAAAAAATATCAAGT
TTCAGTGAAGAAAATTGAAAATCTCTCACATACTGCCATACAACATGAAT
TAGAAAATGAGAAAAAATCCGAAGACAATAAAAAACCCACAAGAGTTTTG
TTTTATGGAGACACACCTTCAGAAATATCAGAAATAGAAGAAAAATCTGA
TACGAAATTCTCAGATATTTTTGAAACTGTAAAAAGAAACATGCAAACAG
AAATAAAAGTAGATACCAACAATAATTCAAACAAAGAGGATGCTCAAAAG
TCTGAGCTACTTGCTAAGTTAAAGCAAATAGATGATAATAAAAAAAAAGA
AAATTTGAAAAGTGAGATGAGCAATCTGGATCTGGTGGATGTGGAGTTGA
AAACTATAATGAAAAATGAGAAAGAATCTGATTCAGACGATCTTGATATG
TTTAGTACTAGTGGCAGTAGTGTGACTGGTGATAGTGATGAGGACGATGA
TGATGAAGAGGAGGAATCATCAGATAAAAAAAGCGATTCTTCAAGTCCTT
GTTTGTTAGTTCCTCGTAGAAATGCTAAATTAACGACATCTAAAATGCCT
CAAAATAAAAACAGTAAAAGAGAAACAACGCAATTTGAATTTCTAGGGTT
CTCAGTGAATACCAACAATAGCAACAGTGAGGGGCAGAAAGTCAATTGTA
AGATGGGAGATCAAGAAAGGAACAATTTATCATCCGATTGCGAGGAAATT
AAAATTGATTGATATTATACTGATTATTGTTTCGTTAATAAATGGGTAAT
GTATGTATTATAACTACTAACGTATGATATTTTATTTCATTTGTCAATTA
CAGTAATTCTAGC

protein sequence of SMED30028234-orf-1

>SMED30028234-orf-1 ID=SMED30028234-orf-1|Name=SMED30028234-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=607bp
MNIKKENDFVIKVKTPTTLISKHISLSEKTNRRSVSQASKSSKNNRSSQS
LSYSDSFDSDSPVKSSAVHRVKRISKPAFHIASTQNKKIKKAPFGISNFS
KTAESTQKLLEASQLEINELISENADLRRQLLESSNELKTVQNQNKIQER
YLNKVNSRDSDVPTIISRYNSELHSLQEQLRRKKEKLDTVERQKTDLDFE
LVKAKKKIEKLKALVADRQLQEREDLSKKIANLNESLEAEIKKNSDLERS
MELLTKKYSYQMALETNKFKELKQSYTETLMELKNIRSSLKEKNKLLEMQ
NIYSKRVVRAPHRLSGKKGKNVTLEDSNFPVSKISTVPPLKSSLRKEERH
NSEHEEASQIKINLKKKYQVSVKKIENLSHTAIQHELENEKKSEDNKKPT
RVLFYGDTPSEISEIEEKSDTKFSDIFETVKRNMQTEIKVDTNNNSNKED
AQKSELLAKLKQIDDNKKKENLKSEMSNLDLVDVELKTIMKNEKESDSDD
LDMFSTSGSSVTGDSDEDDDDEEEESSDKKSDSSSPCLLVPRRNAKLTTS
KMPQNKNSKRETTQFEFLGFSVNTNNSNSEGQKVNCKMGDQERNNLSSDC
EEIKID*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0002089	reproductive organ

Vocabulary: **INTERPRO**
Term	Definition
IPR028933	Lebercilin_dom

Vocabulary: **molecular function**
Term	Definition
GO:0005515	protein binding

Main menu

SMED30028234

Zeng et. al., 2018

▻ Overview

▻ Neoblast Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 4

Owlery

blazegraph-runner